LOCUS       DAWFIT010000001        95699 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFIT010000001 DAWFIT010000000
VERSION     DAWFIT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 95699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.001"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            52..1005
                     /locus_tag="WGQ85_000001"
     CDS             52..1005
                     /locus_tag="WGQ85_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0749524.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WGQ85_000002"
     CDS             complement(1116..1439)
                     /locus_tag="WGQ85_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749525.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WGQ85_000003"
     CDS             complement(1612..2973)
                     /locus_tag="WGQ85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0749526.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGQ85_000004"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WGQ85_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0749527.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5752)
                     /locus_tag="WGQ85_000005"
     CDS             complement(5039..5752)
                     /locus_tag="WGQ85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749528.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTFMLWGMNDPFSQTTDRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5799..6311)
                     /locus_tag="WGQ85_000006"
     CDS             complement(5799..6311)
                     /locus_tag="WGQ85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0749529.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6458..7789
                     /gene="pmbA"
                     /locus_tag="WGQ85_000007"
     CDS             6458..7789
                     /gene="pmbA"
                     /locus_tag="WGQ85_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0749530.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8086..8289)
                     /locus_tag="WGQ85_000008"
     CDS             complement(8086..8289)
                     /locus_tag="WGQ85_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0749531.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8532..8834
                     /gene="clpS"
                     /locus_tag="WGQ85_000009"
     CDS             8532..8834
                     /gene="clpS"
                     /locus_tag="WGQ85_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0749532.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8836..11115
                     /gene="clpA"
                     /locus_tag="WGQ85_000010"
     CDS             8836..11115
                     /gene="clpA"
                     /locus_tag="WGQ85_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0749533.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11162..13033)
                     /locus_tag="WGQ85_000011"
     CDS             complement(11162..13033)
                     /locus_tag="WGQ85_000011"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0749534.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(12994..13365)
                     /locus_tag="WGQ85_000012"
                     /pseudo
     CDS             complement(12994..13365)
                     /locus_tag="WGQ85_000012"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13384..14259)
                     /locus_tag="WGQ85_000013"
     CDS             complement(13384..14259)
                     /locus_tag="WGQ85_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0749535.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14338..14919)
                     /locus_tag="WGQ85_000014"
     CDS             complement(14338..14919)
                     /locus_tag="WGQ85_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0749536.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNS
                     LKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALD
                     INFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTL
                     THSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(14964..15560)
                     /locus_tag="WGQ85_000015"
     CDS             complement(14964..15560)
                     /locus_tag="WGQ85_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0749537.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGG"
     gene            complement(15726..16736)
                     /locus_tag="WGQ85_000016"
     CDS             complement(15726..16736)
                     /locus_tag="WGQ85_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0749538.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16733..18277)
                     /locus_tag="WGQ85_000017"
     CDS             complement(16733..18277)
                     /locus_tag="WGQ85_000017"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0749539.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18350..19354)
                     /gene="rfaD"
                     /locus_tag="WGQ85_000018"
     CDS             complement(18350..19354)
                     /gene="rfaD"
                     /locus_tag="WGQ85_000018"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0749540.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19395..20513)
                     /locus_tag="WGQ85_000019"
     CDS             complement(19395..20513)
                     /locus_tag="WGQ85_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0749541.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20515..21750)
                     /gene="dcm"
                     /locus_tag="WGQ85_000020"
     CDS             complement(20515..21750)
                     /gene="dcm"
                     /locus_tag="WGQ85_000020"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0749542.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21787..22758)
                     /gene="hldA"
                     /locus_tag="WGQ85_000021"
     CDS             complement(21787..22758)
                     /gene="hldA"
                     /locus_tag="WGQ85_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0749543.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22813..23553)
                     /gene="pyrF"
                     /locus_tag="WGQ85_000022"
     CDS             complement(22813..23553)
                     /gene="pyrF"
                     /locus_tag="WGQ85_000022"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0749544.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24079..24726)
                     /gene="adk"
                     /locus_tag="WGQ85_000023"
     CDS             complement(24079..24726)
                     /gene="adk"
                     /locus_tag="WGQ85_000023"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0749545.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24941..25780
                     /gene="htpX"
                     /locus_tag="WGQ85_000024"
     CDS             24941..25780
                     /gene="htpX"
                     /locus_tag="WGQ85_000024"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0749546.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSQGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26081..26641)
                     /locus_tag="WGQ85_000025"
     CDS             complement(26081..26641)
                     /locus_tag="WGQ85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749547.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(26755..27147)
                     /locus_tag="WGQ85_000026"
     CDS             complement(26755..27147)
                     /locus_tag="WGQ85_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0749548.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(27355..27747)
                     /locus_tag="WGQ85_000027"
     CDS             complement(27355..27747)
                     /locus_tag="WGQ85_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0749549.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(27864..28274)
                     /locus_tag="WGQ85_000028"
     CDS             complement(27864..28274)
                     /locus_tag="WGQ85_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0749550.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(28288..28725)
                     /locus_tag="WGQ85_000029"
     CDS             complement(28288..28725)
                     /locus_tag="WGQ85_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0749551.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSIGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(29136..30434)
                     /locus_tag="WGQ85_000030"
     CDS             complement(29136..30434)
                     /locus_tag="WGQ85_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0749552.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPEGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30537..31688)
                     /locus_tag="WGQ85_000031"
     CDS             complement(30537..31688)
                     /locus_tag="WGQ85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0749553.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            32023..33402
                     /gene="norM"
                     /locus_tag="WGQ85_000032"
     CDS             32023..33402
                     /gene="norM"
                     /locus_tag="WGQ85_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0749554.1"
                     /translation="MLLDLNRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            33446..34486
                     /gene="murB"
                     /locus_tag="WGQ85_000033"
     CDS             33446..34486
                     /gene="murB"
                     /locus_tag="WGQ85_000033"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0749555.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            34498..35148
                     /locus_tag="WGQ85_000034"
     CDS             34498..35148
                     /locus_tag="WGQ85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0749556.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            35203..35979
                     /locus_tag="WGQ85_000035"
     CDS             35203..35979
                     /locus_tag="WGQ85_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0749557.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHSSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSSDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECDETALPDPQTESVRQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNILGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            36030..36596
                     /locus_tag="WGQ85_000036"
     CDS             36030..36596
                     /locus_tag="WGQ85_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0749558.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            36616..37506
                     /locus_tag="WGQ85_000037"
     CDS             36616..37506
                     /locus_tag="WGQ85_000037"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0749559.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            37531..38289
                     /locus_tag="WGQ85_000038"
     CDS             37531..38289
                     /locus_tag="WGQ85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0749560.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(38344..39009)
                     /locus_tag="WGQ85_000039"
     CDS             complement(38344..39009)
                     /locus_tag="WGQ85_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0749561.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(39123..40019)
                     /locus_tag="WGQ85_000040"
     CDS             complement(39123..40019)
                     /locus_tag="WGQ85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0749562.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQYSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVCLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            40088..41245
                     /locus_tag="WGQ85_000041"
     CDS             40088..41245
                     /locus_tag="WGQ85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0749563.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(41308..42309)
                     /gene="hemB"
                     /locus_tag="WGQ85_000042"
     CDS             complement(41308..42309)
                     /gene="hemB"
                     /locus_tag="WGQ85_000042"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0749564.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(42492..42776)
                     /gene="yhbY"
                     /locus_tag="WGQ85_000043"
     CDS             complement(42492..42776)
                     /gene="yhbY"
                     /locus_tag="WGQ85_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0749565.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            42886..43506
                     /locus_tag="WGQ85_000044"
     CDS             42886..43506
                     /locus_tag="WGQ85_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0749566.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            43570..45537
                     /gene="ftsH"
                     /locus_tag="WGQ85_000045"
     CDS             43570..45537
                     /gene="ftsH"
                     /locus_tag="WGQ85_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0749567.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            45759..46190
                     /locus_tag="WGQ85_000046"
     CDS             45759..46190
                     /locus_tag="WGQ85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0749568.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            46183..46461
                     /locus_tag="WGQ85_000047"
     CDS             46183..46461
                     /locus_tag="WGQ85_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0749569.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            46514..47092
                     /gene="pth"
                     /locus_tag="WGQ85_000048"
     CDS             46514..47092
                     /gene="pth"
                     /locus_tag="WGQ85_000048"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0749570.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            47153..47359
                     /locus_tag="WGQ85_000049"
     CDS             47153..47359
                     /locus_tag="WGQ85_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0749571.1"
                     /translation="MTELKQLIQTESILVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            47356..47718
                     /locus_tag="WGQ85_000050"
     CDS             47356..47718
                     /locus_tag="WGQ85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0749572.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            47949..49364
                     /locus_tag="WGQ85_000051"
     CDS             47949..49364
                     /locus_tag="WGQ85_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0749573.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            49483..49992
                     /locus_tag="WGQ85_000052"
     CDS             49483..49992
                     /locus_tag="WGQ85_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0749574.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNHRSKEMYGRTVVQDWGYAVFGKVVGGFDVVDAIEGVSTKRHGYHDDVPTEP
                     VIITKAEAV"
     gene            50155..51537
                     /locus_tag="WGQ85_000053"
     CDS             50155..51537
                     /locus_tag="WGQ85_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0749575.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSTMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGRRVEFPDGFGLMRTSNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(51557..52312)
                     /locus_tag="WGQ85_000054"
     CDS             complement(51557..52312)
                     /locus_tag="WGQ85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0749576.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(52322..53038)
                     /locus_tag="WGQ85_000055"
     CDS             complement(52322..53038)
                     /locus_tag="WGQ85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0749577.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(53028..53855)
                     /locus_tag="WGQ85_000056"
     CDS             complement(53028..53855)
                     /locus_tag="WGQ85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0749578.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            54140..55048
                     /locus_tag="WGQ85_000057"
     CDS             54140..55048
                     /locus_tag="WGQ85_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0749579.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            55281..58895
                     /gene="recB"
                     /locus_tag="WGQ85_000058"
     CDS             55281..58895
                     /gene="recB"
                     /locus_tag="WGQ85_000058"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0749580.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            58934..59293
                     /locus_tag="WGQ85_000059"
     CDS             58934..59293
                     /locus_tag="WGQ85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0749581.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            59535..60014
                     /locus_tag="WGQ85_000060"
     CDS             59535..60014
                     /locus_tag="WGQ85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0749582.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(60290..61669)
                     /gene="radA"
                     /locus_tag="WGQ85_000061"
     CDS             complement(60290..61669)
                     /gene="radA"
                     /locus_tag="WGQ85_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0749583.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(61858..62922)
                     /locus_tag="WGQ85_000062"
     CDS             complement(61858..62922)
                     /locus_tag="WGQ85_000062"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0749584.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(62997..64214)
                     /locus_tag="WGQ85_000063"
     CDS             complement(62997..64214)
                     /locus_tag="WGQ85_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0749585.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(64211..65545)
                     /locus_tag="WGQ85_000064"
     CDS             complement(64211..65545)
                     /locus_tag="WGQ85_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0749586.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(65560..66321)
                     /locus_tag="WGQ85_000065"
     CDS             complement(65560..66321)
                     /locus_tag="WGQ85_000065"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0749587.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(66339..66761)
                     /locus_tag="WGQ85_000066"
     CDS             complement(66339..66761)
                     /locus_tag="WGQ85_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749588.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(67012..67332)
                     /locus_tag="WGQ85_000067"
     CDS             complement(67012..67332)
                     /locus_tag="WGQ85_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0749589.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(67355..67981)
                     /gene="upp"
                     /locus_tag="WGQ85_000068"
     CDS             complement(67355..67981)
                     /gene="upp"
                     /locus_tag="WGQ85_000068"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0749590.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(68087..68398)
                     /gene="grxD"
                     /locus_tag="WGQ85_000069"
     CDS             complement(68087..68398)
                     /gene="grxD"
                     /locus_tag="WGQ85_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0749591.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(68499..68714)
                     /locus_tag="WGQ85_000070"
     CDS             complement(68499..68714)
                     /locus_tag="WGQ85_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0749592.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(68740..69519)
                     /locus_tag="WGQ85_000071"
     CDS             complement(68740..69519)
                     /locus_tag="WGQ85_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0749593.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(69524..69874)
                     /locus_tag="WGQ85_000072"
     CDS             complement(69524..69874)
                     /locus_tag="WGQ85_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0749594.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(69878..70855)
                     /locus_tag="WGQ85_000073"
     CDS             complement(69878..70855)
                     /locus_tag="WGQ85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0749595.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(70889..72001)
                     /locus_tag="WGQ85_000074"
     CDS             complement(70889..72001)
                     /locus_tag="WGQ85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0749596.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            72187..72888
                     /locus_tag="WGQ85_000075"
     CDS             72187..72888
                     /locus_tag="WGQ85_000075"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0749597.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            73032..74825
                     /gene="lepA"
                     /locus_tag="WGQ85_000076"
     CDS             73032..74825
                     /gene="lepA"
                     /locus_tag="WGQ85_000076"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0749598.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            74968..75987
                     /gene="lepB"
                     /locus_tag="WGQ85_000077"
     CDS             74968..75987
                     /gene="lepB"
                     /locus_tag="WGQ85_000077"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0749599.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            76085..76191
                     /locus_tag="WGQ85_000078"
                     /pseudo
     CDS             76085..76191
                     /locus_tag="WGQ85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(76252..77151)
                     /locus_tag="WGQ85_000079"
     CDS             complement(76252..77151)
                     /locus_tag="WGQ85_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0749600.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
     gene            complement(77717..78649)
                     /gene="cysK"
                     /locus_tag="WGQ85_000080"
     CDS             complement(77717..78649)
                     /gene="cysK"
                     /locus_tag="WGQ85_000080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0749601.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(78843..79448)
                     /locus_tag="WGQ85_000081"
     CDS             complement(78843..79448)
                     /locus_tag="WGQ85_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749602.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(79445..80296)
                     /gene="dapF"
                     /locus_tag="WGQ85_000082"
     CDS             complement(79445..80296)
                     /gene="dapF"
                     /locus_tag="WGQ85_000082"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0749603.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIISRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            80433..80729
                     /locus_tag="WGQ85_000083"
     CDS             80433..80729
                     /locus_tag="WGQ85_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749604.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            80743..81411
                     /locus_tag="WGQ85_000084"
     CDS             80743..81411
                     /locus_tag="WGQ85_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0749605.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(81484..83604)
                     /gene="pnp"
                     /locus_tag="WGQ85_000085"
     CDS             complement(81484..83604)
                     /gene="pnp"
                     /locus_tag="WGQ85_000085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0749606.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(83841..84704)
                     /locus_tag="WGQ85_000086"
     CDS             complement(83841..84704)
                     /locus_tag="WGQ85_000086"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0749607.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPTDQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            84903..85766
                     /gene="rfbD"
                     /locus_tag="WGQ85_000087"
     CDS             84903..85766
                     /gene="rfbD"
                     /locus_tag="WGQ85_000087"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0749608.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFIR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(86241..86474)
                     /locus_tag="WGQ85_000088"
     CDS             complement(86241..86474)
                     /locus_tag="WGQ85_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0749609.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(86640..86840)
                     /locus_tag="WGQ85_000089"
     CDS             complement(86640..86840)
                     /locus_tag="WGQ85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0749610.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(87012..87887)
                     /gene="xerD"
                     /locus_tag="WGQ85_000090"
     CDS             complement(87012..87887)
                     /gene="xerD"
                     /locus_tag="WGQ85_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0749611.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(87939..88379)
                     /locus_tag="WGQ85_000091"
     CDS             complement(87939..88379)
                     /locus_tag="WGQ85_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0749612.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            88700..89638
                     /locus_tag="WGQ85_000092"
     CDS             88700..89638
                     /locus_tag="WGQ85_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0749613.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            89767..90060
                     /gene="hfq"
                     /locus_tag="WGQ85_000093"
     CDS             89767..90060
                     /gene="hfq"
                     /locus_tag="WGQ85_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0749614.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            90145..90711
                     /locus_tag="WGQ85_000094"
     CDS             90145..90711
                     /locus_tag="WGQ85_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0749615.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCHLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            90765..91139
                     /locus_tag="WGQ85_000095"
     CDS             90765..91139
                     /locus_tag="WGQ85_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0749616.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPKKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            91149..91643
                     /gene="folK"
                     /locus_tag="WGQ85_000096"
     CDS             91149..91643
                     /gene="folK"
                     /locus_tag="WGQ85_000096"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0749617.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(91771..92250)
                     /locus_tag="WGQ85_000097"
     CDS             complement(91771..92250)
                     /locus_tag="WGQ85_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0749618.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(92470..93207)
                     /gene="ubiE"
                     /locus_tag="WGQ85_000098"
     CDS             complement(92470..93207)
                     /gene="ubiE"
                     /locus_tag="WGQ85_000098"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0749619.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(93250..93618)
                     /locus_tag="WGQ85_000099"
     CDS             complement(93250..93618)
                     /locus_tag="WGQ85_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0749620.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            93738..95357
                     /gene="mnmC"
                     /locus_tag="WGQ85_000100"
     CDS             93738..95357
                     /gene="mnmC"
                     /locus_tag="WGQ85_000100"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0749621.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPEINLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(95354..95503)
                     /locus_tag="WGQ85_000101"
     CDS             complement(95354..95503)
                     /locus_tag="WGQ85_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749622.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT        22932 a        26825 c        23299 g        22643 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcttt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgccacat cgaaatcctt
     2761 gggttcgatg ccgagcagca gatccctgat cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc agacggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcgggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggccgtctg aaacgcggga
     3661 atgttgtgtt cgagtatgct caagcccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaagtgaag attgggaatg
     3781 gaacgccccg aaccgcgcac ttcaccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcaacg atgccgacca cgccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt caggaaaagc attcagcgcg
     3961 tcttgcagca tagactgctc gatttcgcga cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaaggtcgcc cagattcggc
     4321 tctttgatgc cgtctgaaaa ataatcgcgg cggcgcaatt cggcacgcaa cgccgtcaac
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccaca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtatcggcag gcaaatggtg gtcggttaca
     4501 atgacatcca aacccaattc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tcaaccgtaa tcagcaaatc cacgccttgc gcggcggcga tttcggccag ctcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acgaggaaat ccactttcgc gcccatcgcc
     4681 gccaaaccgt ccaaaccgac ggcacacgcc gtcgcgccgt cggcatcata gtcggcgaca
     4741 atcaggattt tttcctgacg ctcaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcagcg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaggcaca aagccgggcg attaaaggat cggcgccggc ggtgagcaaa
     4921 tgattgaaaa cgtcggtatt gacggatcgg gtttggattt tgacggacat agcgtggaac
     4981 tttacagtgc ggatgtgttt gaaatgccgt ctgaaggctt ttcagacggc attgccgttc
     5041 atttgtctga ggaattcgtg gctgcaatca acgccgcgac aggcaaggcc agcaccgcgc
     5101 ccaccgcatc cagtatcaac gtggtgggcg tatatgcaat attttcaaac aacttggatt
     5161 tgtcggtatg ttttttcgta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 gataatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gagacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagttcga ccgcttcaaa ctcaagctgt ttcagcttgg
     5341 cgatgtcggc aggtctgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctggcggg cgattcgagt ttgaccggca gggcttggta ggcaaagcgc gggttgggct
     5461 caaccatttg aaactgcttg tccaacccgg ccttcaaaat gcccgtcagc ttcgccgaat
     5521 cttcaggatt gacgacgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
     5581 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtccttg tcaacgtgtt
     5641 tgcggtcggt cgtttggctg aacgggtcgt tcatacccca caacataaac gtacagccat
     5701 tcagcatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatcct
     5761 ccaatctaaa taacggcgga tgtcgtctga aaatatgcct aaatttccgc gtccccgttt
     5821 tccataacgg ttttcaattc ttgaaacagg gcgcggaaat ttttcggcgg tttgttttgc
     5881 tcctgctctt ttttggtgtt gcggatcaag gtacgcaatt taccggcgtc cgcatgcgga
     5941 aaatccgaca taaactgcgt caacgcgccg tcgtctgcca acagccgtac gcgcgcctgt
     6001 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaagaaac
     6061 gcctcgatgg gcgcgggatc cgtgtcgcgc atcagcctgc cgataaattg cgcctgccgc
     6121 ttgagcgcgc cgttggatgt gattttttta taggcggtta ccgcctcgta caaatccgca
     6181 tccaaaccga tttttttcag cgtatcgttt gagagcttgg tcaacgccat acccaaatcc
     6241 tgcaaatcgt tcatctgctt tttcatttgg gttttgctga tccattcgtc ttcttgttca
     6301 aacatcttga ttctcgaatt tttccaatag cgcattttaa cagaatgccg ccgcgttcag
     6361 acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt tgcccctgcc gcccgaaacg
     6421 gttaaaatgc cgcaaagcat catcaagaaa gatttctatg ctgttcaacc acaccgcttc
     6481 cgaactcctc gacctctgcc gccgcacgct cgacttggca aaagcagcgg gcgcgaccgc
     6541 cgccgaagcc gatttcagcg aatccatcgg acaaagcgtc agcgtgcggc tgggcgaaat
     6601 cgaacaaatc gagttccagc aggacaagtc gctggacatt accgtttacg tcggcaaacg
     6661 caaaggccgc gctggcaccg ccgatttctc cgaacaagcc ctgcgcgaca ccgtccgcgc
     6721 cgccgccgac atcgcccgcc acaccgccga agacggttgc gccggacttg ccgaccccga
     6781 gttgatggcg caacacatcg gcgaccccga cctttaccac gaatgggatt tggatacgga
     6841 agccgccgtc ggcttggcaa aacaatgcga acaagccgcc ctgaacgagg atgagcgcat
     6901 cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac caatacgttt acggcaacac
     6961 ccacggtttt gccgcacacc ggcaaggcac gcaccacagc atttcctgca gcgtcgttgc
     7021 cgccgacgaa aacgggatgc agcgcgacta ctggtacgat tccgcctgcc gccatccgga
     7081 tatggacagc cccgaaacca tcggtcaaac cgccgcccgc cgcactttgc gccgactcgg
     7141 cagccgcagc atcccgacag gcagctaccc cgtcctcttc gataccacca tttcgggcgg
     7201 tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg ctctaccgcc aaagcagttt
     7261 cctgatcgac agcatcggca aaaaagtcct gcccgatttc ctcaacctgc gcgaagaacc
     7321 gcacatcccc cgctctttcc gcagcagcta ttttgatgcg gaaggcgttg ccaccgcacc
     7381 gcgcttcgtg attcaaaacg gcattgtcga aggctatttc ctcagcagtt acagcgcgcg
     7441 caaactcggt atgcagacca cgggcaacgc cggcggcgcg cacaacctgt atttgaacca
     7501 tacgcacgaa acgcaatccg acctgctgaa agaaatgggg acgggattat tggttaccga
     7561 actgatggga cagggcgcga acaccattac cggtgactac tcgcgcggcg cggcgggttt
     7621 ttgggtggaa aacggcgtga ttgcctaccc cgtccacgag attaccgtag ccggacgctt
     7681 gcaggatatg taccgcgaca ttgtcggcgt ggcggatgac gctttgcggc gttcgtccaa
     7741 caaaatcggc tcgattctga ttgcggggat gacggttgcc ggaagctgaa cgccctgccg
     7801 atgccgtctg aagacgaagg cagcacgttt tgccccgtgc ctacccgttt gccgttttat
     7861 gccgcaccgc cttttgccga caacattgca cattttttca tcagcactga ttttccctta
     7921 tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca ggataaggcg
     7981 tgcggggatt ggggtttttc cgccgtcggc aaaacggcag acaaaaacgg cgggacattg
     8041 cctgtcccgc cgttttcaaa tatacatttg accgtacatc aggaattaag cagcctgaat
     8101 gttggcggcc tgtttgcctt tagggccggt ggttacgtcg aaagagacgc gttggccttc
     8161 tttcagggtt ttgaaacctt ccatattgat tgctgagaag tgagcgaaca aatcttcgcc
     8221 gccttcatca ggagtgatga aaccaaaacc tttagcgtcg ttaaaccatt ttacgatacc
     8281 ggttgccatt agaaacttcc tatactcaaa aattaacaaa atcagcaaaa caaggcatac
     8341 ggcaaaccga gccgtctggc gttccttcca gcttgatata ctcggcggat gcccaatcct
     8401 gcttaaccgt ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg
     8461 ggaaaatagg caaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa
     8521 tgggacacac tatgaccgcg caacaccaat ccgacacgct cttgcaccgt ctgaacaccc
     8581 tgccgccgaa acgttacggc gttttcctat tgaacgacga ttacaccacg atggaatttg
     8641 tcgtcgaaat cctgaccgaa atctttatgc tcggacaaga acaggcggta gcggtgatgc
     8701 tcttggttca tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat attgcccaaa
     8761 ccaaacaaca acaagtcatg cagcgggcaa aagccgaagg gcatccgctg caatgtattg
     8821 tcgaggagat ttaatatgct tgcacccgaa ttggaacaga ttttgcagca gctttaccgc
     8881 gaggcgcgta aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc
     8941 gaagaagatg cctccgtgcc gaacgtctta aaactatgcg gcgcggattt gaaagtggtg
     9001 tccgaacagc tcgccgccag cgttgccgaa aacacccccc tgattcccga acacctttta
     9061 gacacggtcg aaacccggcc cacgctcggc ttccaacgcg tgatgcaacg ggcgatggtg
     9121 catacccagt ctgccggaaa agccgcagtc gaaccgttgg acgttttggt cgcgctgatg
     9181 agcgaaaccg acagccacac cgtctatttc ctcaagctgc aatcggttac gcgttttgaa
     9241 gttttgcgct gtattgccca tggctcgccc gatgacgagg atggcaatga ttcagacggc
     9301 ctgggacgcg agggcgaaga agcggaacaa aaaaccggct ccctttccga ctacaccgtc
     9361 aacctcaaca ccgaagtcaa agccggccgt atcgaccctt tgattggtcg caaacacgaa
     9421 atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct tttggtcggc
     9481 gaagcaggcg tgggcaaaac cgcgctggcg gaaggtttgg cacatcaaat cgtcaacggc
     9541 ggcattccag acgcgcttaa agatgccgaa gtgtacgcgc tggatatggg ctcgctgttg
     9601 gcgggcacga aataccgtgg cgactttgaa gcgcgggtca aatccgtctt gaaacagctc
     9661 gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat cggcgcaggc
     9721 agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct ggcaaaaggt
     9781 tcgctgcgct gcatcggcgc aaccacctac gacgaatacc gcaccatttt cgacaaagac
     9841 tatgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt ttccgaaacc
     9901 gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt ccgctatacg
     9961 caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga gcgtttcctg
    10021 cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat tctgcccaaa
    10081 tccaaacaga aaaaagtcat cggcaaagcg caaatcgaaa ccgtcatcgc caaagtcgcg
    10141 cggattccag aaaaaaccgt gtcgcacgac gacaaacagg tgctgcaatt cctcggccgc
    10201 gatttgaaaa acatggttta cggtcaagaa aacgccatcg acgcgctggt tgccgccgtc
    10261 aaaatgtcgc gttcgggctt gggactgccc gacaaaccga taggcagctt cctcttctcc
    10321 ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc ttgcctactc gatgggcgta
    10381 ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc gcgcctcatc
    10441 ggcgcgcctc cgggctacgt cggcttcgaa caaggcggcc ttttgaccga agccgtcaac
    10501 aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc cgacattttc
    10561 aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg caagagtgcc
    10621 gatttccgca acgtcatcct gattatgacc accaacgcgg gcgcggaaag cctcagccgc
    10681 cccggcctcg gctttaccgc caagcgcgaa cgcggcgacg aaatgcaggc catcaacaaa
    10741 ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccgtttgc acccctgtcc
    10801 gaacccatta tcgccaaagt cgtggacaaa ttcctgctcc ggctcgaaca ccggctcctc
    10861 gacaagaaag tcgaagccga attcacaccg gcattgcgca aatatctggc gagaaaaggt
    10921 ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga ttcaggaaaa aatccgcaaa
    10981 ccgctcgccg acgaactcct gttcggcaaa ctatccaacg gcggcttcgt gcggatagac
    11041 tgggatgcgg caaaagaaga agccgtgttg aaatttaaga aaagcaaggt taaacctgaa
    11101 acagaaactg tttaaccgat tgaaaaataa aatgccgtct gaaatttcag acggcatttt
    11161 tttaaatatc tgtccctatt ccggcaaact tttccacaaa cgccgcaatc ttttgaaaaa
    11221 cactttgttt cttcgtcaga tattgcgggt tgagcgggct cattttcggc agggtttcgg
    11281 tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc aaataacgtt
    11341 tggcggcggt ttcgttcagg tcttcggcgg caatcagctc tgctgcttcg cgcctcatca
    11401 cctcttgagc gtaggtgtaa aaggtttcca gtatggcagg aacgtcgggg actttgtcca
    11461 aatccgtatc gttgatgaaa tccacaatca ggctctcttt ggcgcggtgg ccgatgctgg
    11521 cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt tttttgtggt
    11581 gctcgaaaac cagttgcaag atgtaatcca gattgatttc ctgcgatttg agcaaatcca
    11641 cctcaaaaac cacatcgtcc caatcgattt ttgattgttc tttctgttcg cctgcttttt
    11701 ggcggcgcag ccagtcgcgg atgtcgttat aggcagaacg gtagtcctgc actgcccttt
    11761 cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcagg tagtattttt
    11821 cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac tcgcgcagcg
    11881 cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg ccgaagagtt
    11941 tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg aaacgctcgc
    12001 gcaattcttt tgccacatcc aaataaccgc gccgcacttc gcccgtccga tcatcgatat
    12061 agccgttcat gtattcttcg tagctttttt ccaacaccac gtttttggtg tttttatcgc
    12121 cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag acaatattgc
    12181 cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc atcaggccgt
    12241 gatagcgcag gtttttatcg acaaacagcg tgttcagcgt cggcgcgtca aaacccgtca
    12301 aaaacatacc gaccacaatc agcaaatcca cttcctggtt tttcacccgt tttgccaaat
    12361 cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg aaacaggcgt
    12421 tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc gcttccggtt
    12481 caaaggtctc atcaacaatt tcaccgacgg cgttctgctc ttcgttggcc gcaaaggaaa
    12541 aaatggtggc cactttcagc gggtgcaggc tgcctgcctg ttgtgttttg aacgcttcgt
    12601 aatagcactt cgccgcatcc acgctgctga ccgcaaacat ggcgttgaag cctttaccgc
    12661 ccgcattcag gcggtgcgtt ttctgcctga actggttcag gatatattgc gtgatttcgc
    12721 ggatgcgttc ggggtgcagc agggcttggc ggttttcggc agcactcagt ttcttctcat
    12781 cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc actttgaatt
    12841 tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcaac tcacgcccga
    12901 acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc gtaccggtaa
    12961 aaccgaactg gcagaatttc ttaaatttct ttttcaggtt ttttgcgctt cgccgaattg
    13021 cgagcggtgg cattcgtcga aaataaatac aacctgctga tggtaaaccg gcagattatc
    13081 ttcactcttc atcaggttgt tcagcttttg gatggtggta acgatgattt tgttgtcgtc
    13141 tttttccaaa ttgcgtttca agcccgccgt gctttccgaa ccattcacgc tgtcgggcga
    13201 aaaacgttgg tattccttca tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa
    13261 aaccttgtcg ataaacgccg attccgtcgc cagacgcgcc gccttaaagc tggtcagcgt
    13321 tttgccgctg cccgtggtat gccaaacata gccgccgctt tccggtttgc tccaattctt
    13381 tacctacgcc gagctgttga ttttccacaa aatgcgttcg gcggcggcaa tctgatacgg
    13441 ccgcataatc agcagcgtat cattcgcatc gaacacgctg taatgcagca atacgcccag
    13501 caatacgctt ttctgcagga acgtggcggt aaagtctttc aaatccttaa tcggatgatt
    13561 gtccgaccgc gcccaattca tcgtgaaatc aaagctgttt ttgtcgcgct tggtggtgtt
    13621 ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag atttgcagga atttgaacag
    13681 cgaattttcg ctgttgaagc tctctttgct gtaacggtgc acctgattga atgcttcgcg
    13741 caccgccacg ccgcgctttt tcaattcaat ctgcaccagc ggcaggccgt ttaccagcac
    13801 ggtaacgtca tagcggtttg catacgtgcc cgtctgctca aactgattga taatctgcac
    13861 atggttgcgg gcaaggtttt tcttgtccag cagataaatg tttttcagac ggccgtcatc
    13921 aaaagcgaaa tcataaatat ggtcgtcatg gattttgcgg gttttatcgg taatgttttc
    13981 agacggcctg tccagatatt ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac
    14041 atcgttcagc cgctgcaact gcgcgcgcag gttttccagc agcctgcttt ggctgttcaa
    14101 atccttgcgg tattcgtaac cctgattctg caaatcggcg attaactccg cttccaactg
    14161 gttttccgat tggtagctgc ccgactgttc gattttttca tattggtcga gcacgatgaa
    14221 attcggcgtt tcggcgatgg gtttggtttc gaggttcatg ggtcgtcctt tgcagtatcg
    14281 ggttttaaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc tgctttttca
    14341 ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag
    14401 ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc agggtatcga atttgtctag
    14461 gatggcggca attttttcct gttcggggag tggtgggatg gggattttta atttctttaa
    14521 ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc tctgctccgc
    14581 aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa attcatcttt
    14641 aattgtaaaa ttggtcagtt gttggttact aatataatta acttttatta acgcatgctc
    14701 tcctatggtt gccgttgtcg acatcattat tgatttagca ggaaacagct tgccgccctt
    14761 aactgcgctt tttgatatat gcttcaatga attatctaaa atacggctat tttccctaat
    14821 atcttccatt ctaaaccaag ggattgagcc attttcccaa tattctttat ttgatttgga
    14881 aggggtatag ccattcttca agtcaaacac ctcccccaac gtcttccaaa ccacatcttt
    14941 cagacggcct ttatagccat cagctatccc ccccccccga tttgattgtc aaaatctaaa
    15001 agaaagtcgc ggtaataccg gtattggcgt ttgcgcaggg ctaattcagc ttccagctcg
    15061 gtgaatttgt caagtatttt tacaattttt tgttgggttt ccaggggtgg gatagggatt
    15121 aataattttt tcaagttact ggcatttaga gcaggaatac ctgtaaatgt acataaggca
    15181 tgaatctctt gttcaaattc aagtaatata tgatagagaa aacgaacatc caattctgtt
    15241 ctattcttaa tagtaactgc ataatttaag ttgccacgaa aatatcttcc ttcttgccac
    15301 gttattgagc cgactcccgc tcctcttgtt gtgatgccga tagggtcatt ttcagtgttc
    15361 cattcatcaa tatagccaag cggctctttg ccactgttaa taactggata actaccaata
    15421 ttatttgaga ttttctgctt acttacagct tgtcctgttt ttatgatagc tatcccattt
    15481 tctccaccca acggcttcca ctccaccggt gccgtctgaa tcatctcaat caattttttc
    15541 gctttgcttt gcatatccat ggttgttttc cgtttctttc ccgctgtggc gggatgtggg
    15601 gcggttgtgg ggtgcttcgg ctcactctgt ccgtttgccg ttatttctgc gggctgaagt
    15661 ccgtcctgct ttcaggctgc ctgcaatggt gtgcaggctg ctttttcagg tagcctgaat
    15721 ggtagttatt ttgtgcggtc ttttttctgt ttgatctgtt gcacggctgt tttttccaac
    15781 gctttaacgc tttccagata ggcttgctcc accggcgaca ggatgcgcgc ttcataggcg
    15841 cggtattcgc gttcggcttt ctgctctgcc tgtttgcggc tgatgctgcc tgcaccctgt
    15901 aatgtgcctt gtccgtacag cccggaaaat ttgtccaatt cggctatcca gtcgcgcata
    15961 tacatggggc tttgctcctg cgctttgatt tccgccaggt cgaagaaggc ggaaaccaga
    16021 cggttcaggc ggaacagttc gtcttcggtc agatagtttt tggcgatttt ggcttcattc
    16081 agcgtgggga ttgcgccttg aaaggtggtc agccccataa agtctttgct gctgtcggca
    16141 cggctgtata tcagctcagc tgcggtttgc cggctggcgg catagtgcag tttgttttga
    16201 acggcggcaa aaaaggtttg gctttcgctg cttttggggt tgtagtcttg gctggtggca
    16261 tataaatcaa gcacttgccg gtatagggct ttttcgctgc tgcggatgtc gcgaatgcgg
    16321 ttgagcagtt ctttccaata gtcgccgccg cctgtgcctt tcaggcgttc gtcgtctatg
    16381 gcaaagcctt tggtcagata ttcgtccagc cgttcggttg cccattggcg gaattggatg
    16441 ccccgcgcgg aacggacgcg gtagccgacg gcaataatca tgggcaggga ataatgggcg
    16501 attttgcggt ttaccttgcg cccgttttca ttttgaactg tcaactggaa gttgacagtt
    16561 gccttctctt ccaattcttg ctctgcaaga atggttttaa tgtgtttgct gatattttgt
    16621 ttggtggttt ggtacagttc tgccatgtcc gcctgcgtca gccaaagctg tccgccaaat
    16681 tcctgcaagg caaattgggc agtgccgtcg ttggcggtgt ataggatgat gctcatgctt
    16741 caatctctgc aatcacttcg tcaatttcac gccgcagccg ttcgattttg gcaacggttt
    16801 cgctgatttc ggcgttaagc tgtttgatgt cgataatttc gcgggtgtct tcgggttcaa
    16861 catagctgct gacggcgagg ttgtagccgt tgtctttgac ggtttgctgg gcggcgtttt
    16921 gggcgatatg cggcacatcg gctttatcgg cgaagagttt gacgatttcg gcaatgtgtt
    16981 cttcggttaa gacgttgttg ttggtttctt ttttaaagaa gccgcctgcg tcgatgaatt
    17041 ggatgtcggt attgtctttg tgtttggaca aaaccagtat attgacggcg atgccggtgc
    17101 cgtaaaagag attgggcgca agggcgatga cggtttccac gtagttgccc tccaccagat
    17161 attggcggat tttctgctct gcgccgccgc gatagaaaat gccggggaat gagacgatgg
    17221 cggcgcggcc tctgccggaa aggtagttca gtgcgtgcag aatgaaggca aaatcggctt
    17281 tggatttcgg ggcgagtacg cctgcagggg caaagcggtc gtcgttgatt aaggtggggt
    17341 cgccgctgcc tatccagttg atggaatagg gcggattgga aacgacggca tcaaagggtt
    17401 tgctgtcttt gagtttggga ttggtcagtg tgtcgcccaa ttcgatgtgg aatttgttgt
    17461 aattgacgtt gtgcagaaac atattcatgc gggcgaggtt gtaggtggtg tggttgattt
    17521 cctgcccgaa gaagccttct tcgatgatgt gctcgtcaaa ctgttttttc gcctgcaaga
    17581 gcaggctgcc cgagccgcaa gctgggtcgt agattttgtt tactttctcc tgcccgtgca
    17641 ccgccagccg cgcaatcagc ttggatacgc tttgcggggt gaaaaattcg ccgccggatt
    17701 tgcctgcgtt ggcagcgtag ttggaaatca ggtattcgta ggcatcgccg aaaaggtcga
    17761 tgtggtagtc ttcaaaactg ccgaaatcga gttccgccac gccttttagg acggcggcaa
    17821 ggcgtttgtt cttgtcggca acggtgctgc cgagccggct gctggtggtg tcgaagtcgt
    17881 caaacaggcc tttaatgtct tgttcggacg gatagccgga ggcggagctt tcaatcgcgg
    17941 taaaaatttc tttcagcttg gtgttgagct cttcgttttg atgggcttcg gcggcaatat
    18001 tgcaaaaaag ctggccgggg tagatgaaat agcctttgac tttgacggca tcgtctttga
    18061 tttcgggcgt gatgatgctg tccggcatag cggcgtaatc aatactgctg tcgcctgcct
    18121 gcatatagtc ggtaaagttt tcgctgataa agcggtagaa aagtgtgccg agaacgtatt
    18181 gtttaaagtc ccagccgtcc accgcgccgc gtacttcgtc ggcaattttc caaatttggc
    18241 ggtgcagttg ggcgcgttgt tgcatttcgg tcatcatcga aatccatata aaagttaaac
    18301 aaatcgaaat cgcctgatat tttcagacga ttttttacgg gcattcaaat taagccaaat
    18361 tttccagcat ccatttgacg tagcgctcga cgcctgcttt gacatcgaaa aattcttccg
    18421 tataacctgc ctcgcgcaat ttggtgatgt cggcttgggt gaagctttgg tatttgcctt
    18481 tgagcgcgtc gggaaatggg atgtagcgga taaattcttc ttctaccaac tcttttaaac
    18541 tcatttcagg tttgccttcg gcggcgcggc aggcgttgac ggtggcagcg gcgagttcgt
    18601 taaactgttg gctgcgacct gttcctaggt tgtagatgcc ggaaagttcg ggatggtcga
    18661 agaagtagag gttgactttg gcgacgtctt cgacgctgac gaagtcgcgg gtttgttcgc
    18721 cgttgccgta gccatcgttg ctgccgaaca ggttgacgta accgtgttcg cggtattggt
    18781 ggaaatggtg gaaggcgacg gatgccatgc gacctttgtg ttgttcgtgt tgtccgtaaa
    18841 cattgaagta gcggaagccg acgacttggg cggtcagcct ttctttcatg cggcggcgca
    18901 atacttggtc aaacaggaat ttggaatagc cgtacacatt aagcggtttt tccagttcgc
    18961 gctcttcgcg gaagatttcg cctttgccgt aaaccgccgc actggaggca taaaggaagg
    19021 gaatgcgttc gtcctgacac cagtccaaca aatccagcgt gtactggtag ttgttttcca
    19081 tcatatacaa accgtcgtga ttcatggtgt cggaacacgc gccttgatgg aaaacggctt
    19141 cgatgttttg ataaggtaaa atgtgttccc tcacttggcg gatgaattca tgtttgtcga
    19201 gataatgggc gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg ctcaaattat
    19261 cgacggcaac aatgtcagta ataccgcgtt gattaagtgc tttgacgatg ttgctgccga
    19321 taaagccggc cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt tttgagtaaa
    19381 attaataaac ttagttacaa gaaatcatcg atattgtcct gaaacacaaa tttgcgatga
    19441 tattccaaat atttttctct tccttcggga tatattctca tcttattttt tgcatttgga
    19501 ttgatattaa gtttttccca tgtgtaacgg cttagtcgag taccgcaaat caaacgacca
    19561 tcatccaaga aagtaatata accttgatcg aacaaccaat cataagtcgg cgttaaagct
    19621 aacccattta aataatctaa tgcctctttc tcatttttct cagtaataca aaccatataa
    19681 ggtttaatat gactagccct taacaaaatc tcatctgtaa ttaatgtaaa tggacattgc
    19741 ggcatatgat ttataacatc cttgcgatat ttctgctgcc cttttctact tttctgttta
    19801 atgatttctt gctctacgaa ttgattaaat tctgaagccg gtatttctaa tttttctctt
    19861 gataagagtt gcgggtgcac gatagagcga aattgataat ccaaaaatat tcggaaataa
    19921 aataatggtt ctgaatcttc tatatctttg acaggcaata gtttaagaat tgataaataa
    19981 ctaattttgg gcaatacaat cttacgccaa attgaccata tatcatcctc cgaacggata
    20041 taacccctac ggtaaccttt agaattttcc aaatggtctg atacatcatg gattgaaaag
    20101 aaaatatttt ctgtaggcag attattaata cttaaaacgt aatcatcata aaaccttgaa
    20161 atatcatgat gatatttctg ttcttgtttg tgatattcaa cttttgcatc aaccaaataa
    20221 cgctgtaaat ttttcttatt aaaaaaacaa tttttctgaa tgagaggata ttctaccctc
    20281 cttccatttt ttgtctcagt aatcccccaa ttttcatact caaaaaatgt acttagtttt
    20341 cgaccactat catcaatata actacctaca tatttgcgtg cttccccatt accaccaaaa
    20401 acagataact tgttattcct gtgaataaaa ctatcttccg cacgtaaatt ttgaatacta
    20461 tcgacaataa aatactctat gttttcaata gtaattcttt gaaaatccgg cattttactc
    20521 tccataaagc gtaatatcca tttgttttaa tatagcgatt aatacatcaa caacaatact
    20581 gtttcccgct tggcgataca tttgagtatc actaactaaa attttaaaat catctctaaa
    20641 acccattagc cgcaaacatt ccctaggtgt taatttacga atacgtccac gattatgagt
    20701 aacataatta tcaaccccgg cacgatgcat tttatgcata gtttgcaata atgggcgagc
    20761 tacaggcaaa tcagtttccg tactggtttt aaaatttttt gttcctcctg ccaaaacata
    20821 atttttgatt ttttcagaaa gataatattt ttcctcaacg ttatttacat caaaaataaa
    20881 gtcatcaaat tcagattcgc gggctgcctg aaaaataaaa tcaccatgcc aattaaattg
    20941 ttgatttgct ttttggcata aggcaatttc tccattaatc tgtgtataac gtttttgtct
    21001 atttttagaa ctggtaacaa atttcacacc cttttcacgt aaaaaatatt tgctatcagt
    21061 atagtcctcc aaaaaatctt gcatagtatg ttctaattca atcttttcag gaaactgaaa
    21121 accatttata ggaggggtat gaaagccaac aacaaaaata cgctcacgat gttgaggaat
    21181 cccataatcc ttactattca ttatttggaa atataagtca taaccaagtg aataaaaaac
    21241 actttttaca actttccaag tttttccatt atcatgattg agcaagccct ttacattttc
    21301 ataaataaat atctttggtt ggacttcatc cacaacgcga gcaaattcat agaataatgt
    21361 tcctcgtgta tcttctaatc ctgcacgttt gccaaccatg gaaaatgctt ggcaaggact
    21421 gcctccaact aaaatatcaa cttgatttct aaactttctc gcatcaaatt gagtaatgtc
    21481 gttatgccaa aaatcttcat ttaatttata gtttccaaga taactttttt taacgtatgg
    21541 atcaatatct cctgcaaaaa caatggtatg gtttaaattt aatcggtgga atgcctgttc
    21601 aaccgcacca atcccgctga atacggttgc taatctaata tgtgaatcag gtttaagaaa
    21661 agttttagat ttccaacctt gttgactggg aaagagcaaa gttttttgta atcgagtatc
    21721 gtgtgtctgt gccattgtcg aaatagtcat acttatatcg ttctgtttat cttatcaata
    21781 tgaaaactac attgttgatt gccctgacaa tgccttgatc aattcggcaa acgagcaaac
    21841 cgccgtaccg agtttcgcca cgacaacccc ggccgcagta ttggcaaggt acatggcttc
    21901 gggcatggtg cagcctgccg ccaaacccaa gcccattccg gcaatgacgg tgtcgcccgc
    21961 accggatacg tcgtaaactt cttgggcgcg ggtgggctgg taaatcggtt cgccttcgct
    22021 gaacaaggtc atgccttctt cgcttcgggt cagtaaaacg gcggtcaggt cgaggtggcg
    22081 gcgcaggttt tgcgcttttt cggtcagctc gctttcgttt ttccaactgc cgaccacttc
    22141 tttcaattcg gcgcggttag gcgtaatcag agttgcaccg acatattttt cgtaatcgtc
    22201 gcctttgggg tcgattaaga cggttttgcc ggcgtgtttc gcccaatcga tcatatcgga
    22261 gatatgcgac aggccgcctt tgccgtagtc tgaaaaaatg attgcgtcgt attcgggcaa
    22321 gatttcgcgg tatttctgct tgatttgttc caacacttcg cagttgggat gttcttcaaa
    22381 atcaagacgg ataagctgct ggttgcgggc gacgacgcgc agtttgacgg tggtggcgat
    22441 ttgtttgtcg cgcatcagat aggaggcgac gccgtcctgc accatcagcg catcgagcgc
    22501 gtcggcggct tcgtcgttgc cggttacgga caacagccct gccctgccgc ccaacgaagc
    22561 gatgttgcgc gcgacatttg ccgctccgcc cgcgcgttgg tcgattcgtc cgattttcgc
    22621 caccggcacg ggggcttcgg gcgaaatacg ggacacatcg ccgaaccaat agcggtcgag
    22681 catcacgtcg ccgacaacca ggactttggc ttgcgcgaaa cgggatttga gggtttcttg
    22741 ttggaacttg gcggacatat tgcctccgtt gtattttttc agacggcctc agcctgtaag
    22801 gccgtctgaa aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc
    22861 taccggatcg gcagcttggg ttacaggacg acccattacc aaataagtcg aagcggcagc
    22921 caaggcttcg gccggtgtca tgatgcggcg ctggtcatca ttattgccgg caacgtccaa
    22981 gcggatgccg ggcgtgacca agacaaaatc ttgtcccaac tcgcggcgca gcggtgcggc
    23041 ttcttgggcg gaacaaacca cgccgtccaa gcccgaactt tgcgccagtt ttgccaagcg
    23101 gatgacttgt tcttcagggg cggtgttcaa accgatttcc gccaaatcgc tttgttccat
    23161 gctggtcaac acggttacgc cgattaagag cggctttgta ccgtatccgg caacggcttc
    23221 tgctgcggct tccatcatac ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc
    23281 catatcggca gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa
    23341 atcgaggaaa agtttgaaac cttgattgat taagctttct gccaaattgc gtcccgtcgc
    23401 ggtaaacagc tctttgccga ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa
    23461 tccgagcgtg tctttttcgt tggaaaaatc aagggcgacg atgacggggg tgcgttgttg
    23521 cggagtttgg aagtcggaga ttaaaggatt catggtgttt tcgtttcggg ttttcagacg
    23581 acctttatcg tctttttcgc agtatagtgg attaacttta aaccggtacg gcgttgtctc
    23641 gccttagttc aaagagaacg cttctctaag gtgctgaagc acaaagtgaa tcggttccgt
    23701 actatctgta ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac
    23761 aacgtaaatt caaaccgtag gtcgggcatt gatgtccgac ctacgcggac tcaagcctcc
    23821 gacaaactgt ttcggataaa tttgaaccgg tacatcagcc gtcattcccg cgcaggcggg
    23881 aatccattga taaaactcag ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg
    23941 gattcccgcc tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa cacgctgttt
    24001 atcaaaaaca ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt
    24061 gggtacgacc ttttcgattt atttgcccaa tgcgcccaat acttcggctt tcacggcttc
    24121 taccggctga gtgccgtcaa ctttgatgta tttaggcgcg tgttcgcctt ccagtttgct
    24181 gtaaaaatcg accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt ttttcacggt
    24241 ttcttctttg tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcgt ctttgccttc
    24301 aactttgggc gggttgtagg taacgtggta agtacggccg gaagccaaat gcacgcggcg
    24361 gccgctcata cggtcgacaa tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa
    24421 atccacgcct gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga aaccgtcgaa
    24481 caggaaaccg tttttgcagt cgtcttgcgc gatgcgttct ttgaccatgc cgataatgat
    24541 gtcgtcgcgc accaagccgc cttcgtcaat gatttttttc gcttccaaac ccaagggcgt
    24601 gccagcctta atcgcggcgc ggagcatgtc gccggtagag atttgcggaa tgccgaacgc
    24661 ggcggtgatg aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc
    24721 tttcatgaag tgatcctttg aaatgtgttt attggaatca aacagatggt ttacgattct
    24781 acacggcttg gcacaatagt tcaaaaacca actgcacaaa ctgccatttg caagcaaatg
    24841 ttttgcatac ctttgccgag tctgatattc acaagccggc aactttttgg gacaatcccc
    24901 aaaatttcaa aacatcacac cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt
    24961 tttggctacc aatatcgctg ttttggtcgt aatcaacatt gttttggcgg ttttgggcat
    25021 caacagccag ggcggcacgg gcagcctgtt ggcgtattcc gccgtcgtcg gcttcactgg
    25081 ttcgattatt tcgctgctga tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt
    25141 tatcgacacg ccgcgcaccg aagaagaagc ctggcttttg aacactgtcg aagcccaagc
    25201 gcggcaatgg aacctgaaaa cgcccgaagt cgccatctac cactcccccg aacccaatgc
    25261 ctttgccacg ggcgcatcga gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga
    25321 ccatatgacg cgcgatgagg tggaagcggt gttggcgcac gaaatggcac acgtcggcaa
    25381 cggcgatatg gttacgctga cgctgattca aggcgtggtc aatacctttg tcgtattcct
    25441 gtcgcgcatt attgccaacc tgattgcccg aaacaacgac ggcagccagt cgcagggaac
    25501 ttatttccta gtcagcatgg tattccaaat cctgttcggc ttccttgcca gcttaattgt
    25561 catgtggttc agccgacaac gcgaataccg cgccgacgcg ggcgcggcaa aactggtcgg
    25621 cgcgccgaaa atgatttccg ccctgcaaag gcttaaaggc aacccggtcg atttgcccga
    25681 agaaatgaac gcaatgggca tcgccggaga tacgcgcgac tccctgctca gcacccaccc
    25741 ttcgctggac aaccgaatcg cccgcctcaa atcgctttaa accgatttga aacggcaaaa
    25801 accgtacgcg caagcagtac ggttttttgt atatgctgtc tgaaaacatc aggtttccat
    25861 accgacacca accgtatcca tttccgcacc ggatacgccg cattgatata ccctgataaa
    25921 ccgtatccgg acagaaaata tagtggatta acaaaaacca gtacggcgtt gcctcgcctt
    25981 agctcaaaga aaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat
    26041 ttatactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatcccct atatcagata
    26101 aaacagcggt aaaattgccg tctgaaacca actctgcgcc atacgggaca cgctctgcac
    26161 gtctatttgc ctccgatata tcctccctac tgtaggaacg tctgccgatt tgcacgaaat
    26221 cggcacctct agcacccttc aggatggcgg acgttatccg ttcttgggat tctgcaagac
    26281 ggtcgacggc attatacaga cgctcggtct gatacccttc cgaagcaacc tgcttcaggt
    26341 attccaaata accgtaaccc aacgcccctc cgacaagcag ggtcgtcaac gctaccagcg
    26401 ttgccgaacg gattttaggg ggcatatctt gaagcatatc tctaccgaac cgaaccaaag
    26461 tctctttaaa gtcggactct acacgcgtag aaccctcgga aatcttgaag ttcaactcat
    26521 actgcttccg tttgtccgct gtcaaccgct tgaggttgtc atccccgtat tggacggttg
    26581 acacaaccct gtacagactg tcctgtattt cgaccagcgt ctgtgcaaag ttggcggtca
    26641 taactggatt cccaaccctt gccgccgatt ctgatactca aaacggtatc caggcgttcc
    26701 agcagggctt cgggagacag cagtgcgtat tcttcaatat tttccatagg ttgattatag
    26761 cagaacctta tacctgttac agacaaggtt gtcgggattt gaacgtccgt cgagcttggt
    26821 tcgtatagag ccgtaggggg tgtttagggc tttggcggca gcttgtacgc tggggaacgt
    26881 gagtgtttcg cctgtggcgg tgtcgaggat ggagacaggt ttttggtttt ggggaggcgt
    26941 attttccttc attttaagtt tcccgcactc cggacaccca tactttgact ttatagctgt
    27001 gtcgtaatac aaaagttgga caaccccgtg cgtaggacat ctaacactgg aaggtttata
    27061 aatgccactg aactccaaca attctaaatg tggaaatttc tcttgaaagc gtgatttggc
    27121 ggtatcgaaa gtcattcgct tacccataat ttgtctccat aggttatgtg caaatggatt
    27181 acacaaccaa aatatagaaa ttttcaaatt ttttgagacc tacctgaaat cctgcgcaag
    27241 cacaatgtaa agaaaaatgg tatacgtctt tactaaatct ttacataaat taactctaac
    27301 cgggatttga acgtccgtcg agcttggttc gtaggattta gggtgttgtg aaccttacct
    27361 atcgtaccca agcactttgt acctgtttga aataaggttg tcgggcgacg ttcgaccctt
    27421 gagcctgtga ttgatttgtt ggaaatgtac acctaaagca gcacctgcgg cagtaacgct
    27481 tgggaacgtg agtgtttcgt tggtcgttgt gtcgaggatg aggaggggct tccctacatt
    27541 tggaggtgtt tgggtttttg acatcttagc acactcagga cacccgtact ttgactttat
    27601 aagggcatta aatgtcgaat aacgaacgtt cccgtgcata ggacaattaa cggttactgg
    27661 gtaattcaca ccgtcaaagc cggttagaga gatattaggg aatatatcag taagttcaga
    27721 agctgcttgg ttgaaagatt tggccatttg agactccaaa attgattgtc aaatggattt
    27781 aatagcaaat ttataaaaat gtcaaatttt tcgagaagca cctgatagtg gcgaggggta
    27841 tacactgtta aaaagtgtat acgtcattta gtgcagatgt gaaccttata cctccctgca
    27901 agtaaagtct ctgtgtgaac ccgacctttt atacggtcag tgatagctgc tgggtttata
    27961 cccaatgacc ttgcagcaga tgctcttgag gggaatgtaa gtgtttcgcc agttacagta
    28021 tcctccaata ctgtattttt agataattta tctattgtat cttctggaat tttgtgggaa
    28081 ttgacaccat aagatgcaca ttttggacac ccacttttag agtccaacat acttttgaat
    28141 gtagagcaag taaccctccc gtgtatagga cagacgatta ccgtcgggta acgaacgcca
    28201 ttgaactcca caagattcaa atgtggaaat ttagaggtta gcttagaaac tgcttgagta
    28261 aatgtaagtg ccatagtggt tgtgaactta ctttaccaac ctataacgat acctaatcaa
    28321 tcggtcaaac ggagtgcgtc cgcttaaacg tacagctaaa gtgctgttgg agcaaccgat
    28381 aaatttactg gcagccagtg tacttgcaaa ctcgtagatt tcccctgtct ctgtgtcctc
    28441 caacctaatg gcaacggcgt ttggagaggg cttttcggga tttcgagtac attcaggaca
    28501 tcctattgag gatttcatgg cagcttgtag attcccgtaa acaaccgacc catgcaaagg
    28561 gcattggatt gttaccggtt cgtagttttt agtgaacttc aaaaccgtct tatcaggaaa
    28621 tttactggaa aaccttaact gaacatcttg tagcgagagt gattttggca ttatagacct
    28681 ttctaaaatg aaagcattat acaaccaaaa tatagaaatt ttcaaatttt ctctgaacgc
    28741 actggtatcc gatggagcta aatgtaaaga aaagggtata tgtccttact aaatctttac
    28801 acattttaac tgtatagtgt ttgacaaaca acaggcatag gcgcataatg cacccgtagt
    28861 tagatagata gtaaggagat aaccaatgac ttagcacctg cccccctctc tctcgcgcac
    28921 cccgcccctc gcttgcggct aatgggcttt gcgcgaaata tacactttca tctcctgcat
    28981 ggcttaaccg cctatgcaat agtacacaca tcaaccaatc aggctggcac gtcttggcat
    29041 tggctgacgt accattgtag cccgaaatgt tgatacccta ctatttatga ccaacggcgg
    29101 attagggctt cagacggcaa ttttactgca aaaccttatg cgaacggatg atgcagcaca
    29161 atcgtttctt cgcggtcggg gccggtggag acgatggcga ccggcgcgcc gcagacttct
    29221 tcaatccgtt tcaaatatgc tttggcatta tcgggcagtt cctcataatt tttgatgccg
    29281 aaagtcgatt ctttccaacc ctccattgtt tcgtaaatcg gtttgcaggt ttccaccgca
    29341 tccgaaccgc aaggcaggat atcggttttg ccgccctcgg gcaattcgta gccgacgcag
    29401 atattgatgg tttcaacgcc gtccattaca tcgagtttgg taatgcacat accggaaatg
    29461 ccgttgattt ggatggagcg tttcagggcg gcggcatcaa accagccgca gcggcgtgcg
    29521 cgtccggtta ccgaaccgaa ttcgtgtccg cgttctgcca aacctgcgcc gacttcgtcg
    29581 aacaattcgg tcgggaacgg gcccgaaccg acgcgcgtgg tataggcttt gacgatgccc
    29641 aaaacataat ccagcatttg agggcctacg cccgcgcctg ccgaagccgc gccggcgagg
    29701 cagttggacg aagtaacgaa ggggtaagtg ccgtagtcga tgtccaacaa cgcaccttgc
    29761 gcgccttcaa acagcagttt ttcgccgttt ttgttttttt cgttcaacac gcgggacacg
    29821 tcggtaatca tcggcgtaat gcgcggcgcg actttttcga taaccgccat cacgtcttcc
    29881 gctttcaccg actcggcatt gtgcagatgt tgcagttgga cgttgtaata agcaaggacg
    29941 gcatccagtt tttcacgcag tttttcagga tgcagcaaat cggcggcgcg aatggcgcgg
    30001 cgtgccactt tgtcttcgta ggcagggccg atgccgcggc cggtcgtgcc gattttgcct
    30061 ttgccgcgcg atgcttctcg ggcttggtcg agcgcgatgt gataaggcag gatcagcggg
    30121 caggtcggcg cgattttcag acggccttcg acgtttttca cgcctgccgc gttcaactcg
    30181 tcgatttcgc ccaacagggc ttcgggggag acgacaacgc ccgaaccgat gaagcagtcc
    30241 agactttcat gcaggatgcc gctcggaatc aggcgcaaaa tggttttttt gccgccgaca
    30301 accaaggtat gacccgcatt gtggccgcct tggaagcgca ccacgcctcc ggcttcttcc
    30361 gccagccagt caacgatttt acctttaccc tcgtcgcccc actgtgcgcc gattactaca
    30421 acatttttag ccatagccat ataaccttcg atattaaaaa ttattgcgga aatccccaaa
    30481 ccggtgcggc tttgcgcccc gccacgattt gcctttcatc cgccatgaac gggtatttag
    30541 cgtttcacga cctgccaaac gccgtccgtc tttttcagac ggcctgcaag ctcttccgaa
    30601 acattgtgtc cgataccgta atcgattacg acacactgcc cttgttcacg caaggcttcg
    30661 accgcttcgt gcgccgcttc ggcatcttcc gcatcgacca acacggcggg ctgccgttcg
    30721 atggcgggca aacgcccgat aaagctgcgc aagtcgaaac tgaatcccgt tgccgggcgc
    30781 gccctgccga aatatccgcc caatccgtca taacgcccgc cgcgcgcgac cgcgtcgtgg
    30841 aaatcggcgg cataggcggc atacagcaag cccgtgtggt aattgtcgac gcgcagctcg
    30901 gacaagtcga tatggatttc acaatcgggg aatgcgtcgc acaccgcctg caattcgccc
    30961 aacgcgccgc cgaccgccga caaatccggc aaccgtccgc gcgcgtcgga caacacttca
    31021 cgcccgccgt acaggcgcgg cagcagcgag aatgcttttg cccacatacc gtccagcttc
    31081 caagccttga cctgcgcttc gaccgtctcg gtatctttat cctgcatcaa ggcaagcagc
    31141 gttgcagact gccccgcatc caaatgcgcc gcatcggaca aggcgcgaaa tatgccgata
    31201 tgccccagcg aaagcagcac tttgcccata tcggcaattt tcatgctttt cagcatcagg
    31261 tctatcagct cgatgtcgcc acggatgtcg gcaaaaccgt acatttccgc ccctgcctgc
    31321 aagggttcgc gcatattcag cagaccgtcg gactgcgcgt gcaacaccgg accggcataa
    31381 cacaaccggt taatcccttg gttggcggat aaaagatggg catcgatacg cgccacctgc
    31441 ggcgtgatgt cggcgcgtat gcccaactgc ctgccgctga gcctgtccgt taccaaaatg
    31501 gttttcaggg aaagccccgc atcgatatgc gtcagcaggg aatgtgcgta ctccatcagc
    31561 ggaggctgta ccagttcata accgtgtacg cggaacagtg ccaacaactg ctccctcgcg
    31621 ctttcaagct gccgcgcgtt cgtgggcagt acgtcggcga tatgttccgg aagctgccat
    31681 gtctgcatga gaatctgcct tctattttat cctgtaaaca caaaagactg cccgatccgc
    31741 aatcagacgg catctttgcc gcaacgcatc acgccgctgt caaaacggcg gtgcttcctt
    31801 ccgattcaga atccgaacag cccctgtccg acggctcaat gccgtcaagt cgttaaaacc
    31861 aaactttacc ataaaataca cacaatctga atatactttt ataccctgta tccgccgtct
    31921 gacggcacgg cattttttta ctatataatg tgcgccatta tttctcatca cttcctgctg
    31981 ccgttttctg cttcagacgg catttttgtt gattgaacac ttatgctgct cgacctcaac
    32041 cgcttttcct tttccgtctt cctgaaagaa atccgcctgc tgaccgccct tgccctgccc
    32101 atgctgttgg cgcaggtcgc gcaggtgggc atcggttttg tcgataccgt gatggcgggc
    32161 ggtgcgggca aggaagactt ggcggcggtg gctttgggca gcagcgcgtt tgccacggtt
    32221 tatattacct ttatgggcat tatggcggcg ctgaacccga tgattgccca gctttacggc
    32281 gcgggtaaaa ccgacgaagt gggcgaaacg ggacggcagg ggatttggtt cgggctgttt
    32341 ttgggcgtgt tcggcatggt cttgatgtgg gcggcgatta cgccgttccg caactggctg
    32401 accttgagcg attatgtgga aggcacaatg gcgcagtata tgctgttcac cagcttggcg
    32461 atgccggcgg caatggtaca ccgcgcactg cacgcctacg cctccagcct gaaccgcccg
    32521 cgcctgatta tgttggtcag ctttgcggcg tttgtgttga acgtgccgct gaactatatt
    32581 ttcgtttacg gcaaattcgg tatgcccgct ttgggcggcg caggctgcgg actggcgacg
    32641 atggcggtgt tttggttcag cgcgctggca ttgtggattt atatcgccaa ggaaaatttc
    32701 ttccgcccat tcggactgac ggcgaaattc ggcaaaccgg attgggcggt gttcaaacag
    32761 atttggaaaa tcggcgcacc catcgggctg tcttattttt tggaagccag cgcgttttcg
    32821 tttatcgtgt ttttgattgc gcctttcggc gaggattatg tggcggcgca gcaggtcggc
    32881 atcagtttgt cggggattct ctatatgatt ccgcaaagcg tcggctcggc ggggacggtg
    32941 cgcatcggct tttcgcttgg gcggcgcgaa ttttcgcggg cgcgttatat ttcgggcgtg
    33001 tcactggtgt taggatggat gctcgccgtg attaccgtgc tttccttggt attattccgt
    33061 tcgccgctgg taagtatgta caacaatgat ccggcggttt taagcatcgc cgccaccgtc
    33121 ttactgttcg ccggcttgtt ccaaccggca gacttcaccc aatgtatcgc ctcctacgcc
    33181 ttgcgcggct acaaagttac aaaggtgccg atgttcatcc acgccgccgc cttttggggc
    33241 tgcggtctgc tgccgggcta cctgctcgcc taccgtttcg atatgggcat ttacggcttc
    33301 tggacggcat tgattgcctc gctcaccatc gccgccatcg ccttggtgtg gtgcttggaa
    33361 ttgtgcagta gggagatggt cagatcgcat aaggctgtct gaaaactcag tacacttcaa
    33421 ttcaaataca gttaaagttc aaaccatgca acccatccga taccgaaccg acctcacccc
    33481 ctacaacacc ttcggtcttc gcgcccaagc ccgagccttt attgcgcttg aacacgccga
    33541 cgggttgcgc gacatcgtcc ggctacccga gttcaaccga gatactgttt tatggctggg
    33601 cggcggcagc aatatccttt tgatggagga ctacgccggt ctggtcgtac atatggaaaa
    33661 caaaggcata cgcgaaattg ctcgttcaga cggcatggtt ctgattgaag cgcaggcggg
    33721 cgaaatctgg cacgattttg tcctgcacgc cgtcgcgctg ggtttgaacg gtttggaaaa
    33781 cctgagcctg attccgggta cggtcggcgc gtcgcccgtg cagaacatcg gcgcatacgg
    33841 cgtagaggcg aaagatgtga ttcacagcgt gcgctgcttc gatttggata cggaaacctt
    33901 tgtaaccctt tccaatgccg actgccgctt cgcctaccgc gaaagcctgt tcaagcagga
    33961 aggtaaaggg cgttatgtga ttgtttcggt cgtatttgca ttaaaaacgc attttgtgcc
    34021 gaacttgggt tacggcgatt tggcggccgc cgttgccgaa ctgagcgcgg gcagggtgcc
    34081 gacggcgaaa gatgtttccg atgcagtgtg tgcaatccgc aacagtaaac ttcctaatcc
    34141 taacgtgctt ggcaatgtcg gcagtttctt taaaaacccc gtcgtcagcg cagaaaaagc
    34201 cgccaccttg ttgcagcggc atcctgatat gccgcgctat ccgcagcccg acggttcggt
    34261 caaactcgcc gccggctggc tgatcgacca atgtcgtctg aaaggctttc aaatcggcgg
    34321 cgcggcggta catgacaggc aggctttggt cttagtgaac aaaaacaatg cctcggcaaa
    34381 cgatgtccgg cagttggcgc aacacatcaa atttacagta tttgctcggt ttcaggtaga
    34441 attgcacgcc gaacccaatt ggctgcctgc ttcgttcagc ctgtaaatcc aaggagtatg
    34501 cccgtgaccc gccccgccaa aatcaatact tacacacgca tcatcgacgc cagccttgcg
    34561 ctcttcaacg aggaaggcga gcgcaacatc agcaccaacc atatcgcggc gcatttgggc
    34621 atcagtccgg gcaatctcta ttaccacttc cgcaacaaag acgaaatcat cgtccagctc
    34681 ttcaaacgtt acagcgaagc cctgcttgcc tacctgaatg aagccgtgtt gccgtctgat
    34741 gtggaagact ccatcaatta tatggccggt atttatgatg tgatgtggga ataccgcttc
    34801 ctattcagcg acgtgaacac cctgcttgca cgcagtgccg aactattggg cgaacacaat
    34861 accttcaccc aagccaaagt ctccccgctc ttggtcaacc tgctgaccca gctcaacggt
    34921 ctgaacatca tccaagccga ccaaaccgcc atgaacgatc tcgccgtcaa tatgtggatg
    34981 gtcacgaaat actggttcga cttcgacagc tccctgcgcg gccgcaccaa gctgaccgaa
    35041 gactccaaag cacgcggcat ccgccgcacc ttaagcctcc ttcgtcctta tcttttgccc
    35101 gaacaccgcg agaaatacga ccggaaaatc ggcaacggca acccgtaaac ccacagcgtc
    35161 cgaatcagcc ttcagacggc acttacccga cggtatcagc gaatgcgcgc agtccccccg
    35221 ccccatatct ccatcacagg cctaggttac ctcggcctgc cgctggcaca aaagttttac
    35281 caacacagca gccgcgtcgc cgccataaaa cgcagcctga cttcggacga catcaacctg
    35341 cccatacatc tcgacacctt cgacctcggc agcagcgatg cctttcagac ggcattgtgg
    35401 cggcaccacg ccgacaaacc cgtctggttc ttcctgctac cgccctcctc gctgacgcat
    35461 tacgccgaca ccgtcaaaca atgggcagag cttgcccggg cgtgcaacgt gcaacacctg
    35521 attttcacga gcagcaccag cgtttacggc gataaggcgc gcgaatgcga cgaaaccgcc
    35581 ctacccgatc cgcaaaccga gtctgtccgc caaatcctcg ccgccgaaca acacctgctc
    35641 gacagcggca tctccaacat cgacatcctg cgacttggtg ggctttattg cgccgaacgc
    35701 catcccgtca gccgccttgt tcaaaagcaa aacatcctgg gcggcaaccg ccccatcaac
    35761 atcgtccacc gtaatatcgc cgtcgaaagc ctgtttcaga cggcatttaa ccccggcggc
    35821 aggcggctga aaaacattat cgaaccgcgc cacccgacac gacgcgaatt ctatacggaa
    35881 gaagccgcca aactcggctt gccggctccc gatttcgcac ccgacgacag cgtgggcaaa
    35941 atcatccgta ccgtttgcga taacggctta agcctgtaaa ataagcgtca acagcaacaa
    36001 acaggcattc tccgccccga tcagaaaata tgtccgccct cctccccatc atcaaccgcc
    36061 tgattctgca aagcccggac agccgctcgg aacttgccgc ctttgcaggc aaaacactga
    36121 ccctgaacat tgccgggctg aaactggcgg gacgcatcac ggaagacggt ttgctctcgg
    36181 cgggaaacgg ctttgcagac accgaaatca ccttccgcaa cagcgcggta cagaaaatcc
    36241 tccaaggcgg cgaacccggg gcgggcgaca tcgggctcga aggcgacctc atcctcggca
    36301 tcgcggtact gtccctgctc ggcagcctgc gttcccgcgc atcggacgaa ttggcacgga
    36361 ttttcggcac gcaggcagac atcggcagcc gtgccgccga catcggacac ggcatcaaac
    36421 aaatcggcag gaacatcgcc gaacaaatcg gcggattttc ccgcgaatcc gagtccgcaa
    36481 acatcggcaa cgaagccctt gccgactgcc tcgacgaaat aagcagactg cgcgacggcg
    36541 tggtacgcct caacgaacgc ctcgaccggc tcgaacgcga tatttggata gactaacctt
    36601 cagacggcat ccgacatgaa cagccctttt cacaacatcg gcatcgtaac ccgccccaac
    36661 acgcccgaca tccaagacac cgcgcacacg ctgattacct ttttgaagca gcacggcttt
    36721 accgtctatc tcgacgaagt cggcataaag gaaggctgca tctataccca agacaccgtc
    36781 ggctgccata tcgtcaacaa gaccgaactg gggcaatact gcgacctggt cgccgtttta
    36841 ggcggagacg gcacctttct ctccgtcgcc cgcgaaatcg ccctgcgcgc cgttccgatt
    36901 atcggcatca accaagggca tttgggcttc ctgacccaaa ttccccgcga atatatgacg
    36961 gacaagctat tgcccgtttt agaagggaaa taccttgccg aagagcgcat cctgattgag
    37021 gccgcactca tccgcgaagg caaaaccgcc gaacgcgcca tcgccctcaa cgatgccgtc
    37081 ctctcccgtg gcggcgctgg acagatgatt gagtttgaag tcttcgtcaa tcgggaattc
    37141 gtctataccc agcgttcgga cgggctgatt gtctccaccc ccaccggatc gaccgcctat
    37201 tcgcttgccg ccggcggccc catcatgcag gcaggattac acgccttcac actcgtaccc
    37261 atctgcccgc aatccatgac caaccgcccc atcgccattc ccgacacgtc tgaaatcgaa
    37321 atcctcgtta cccaaggcgg cgacgcgcgc gtccatttcg acggtcaaac ccatatcgac
    37381 gtgcaaaacc tcgaccgcat caccatccgc cgctaccgaa atcccttacg cattttgcac
    37441 ccgaccgact accaatattt caaaaccctg cgccaaaaac tgcactgggg cgaacaattg
    37501 gtctgaaccg acccttaccg gaaaacacct atgaatccaa aaactccccc cgaccataca
    37561 gaagatgccg tgcgcctctc caaacgcatg gcgcaactgg ggctttgttc gcgccgcgaa
    37621 gccgacggct acatcgaaca gggctgggta acggtcaacg gcaaaaccgc cgtactcggt
    37681 cagaaagttt caccggcaga ccgtatcgaa ctgaacaaga aagcccacga acagcaggcg
    37741 gcacgcatta ccatcctgtt gaacaaaccc gtcggctatg tcagcgcaca agcggaaaaa
    37801 ggctataaat ccgccgccga actgattacc cctgaaaatc actgggaagg cgataccggc
    37861 cgcatccgtt tcgatccgaa acacaaaatc ggcctcgccc ccgccggcag gctggacatc
    37921 gactcggtcg gattgctggt attgactcag gacggccgta tcgccaagca gcttatcggc
    37981 gaaaacagcg gcagtgaaaa agaatatttg gtgcgcgtgc gcggcaaatt ggacgaaaaa
    38041 ggacttgcct tactgaatca cggattgagt ttggacggcg agaaactgcg tcccgcccaa
    38101 gtagaatggc aaaacgaaga ccaactgcgt ttcgtattga aacaaggtaa aaagcggcaa
    38161 atccgccgta tgtgcgaact ggtcggactg cgcgtcgtcg ggctgaaacg catccgcatg
    38221 ggtaaggtca agctcggcag gctgccgccc ggcaaatggc gttatcttgc tcccggcgaa
    38281 tcgttttaaa taagcattaa aaaatgccgt ctgaacgtcc ttacgttttc agacggcatt
    38341 gccttatgcc caaataacgg tttcttccaa gggcctgcgc gctttcgtgg cgatttcctg
    38401 aacgcggtag ccgaatgtcg cggcaacgga cacgccccat tctgccgcat cgaacaaacc
    38461 gaactgcccg gacaatacgc gttccatatc ggcatagttg aaaccttcca ccgggcagga
    38521 atcgatacct gccatcgccg cacccgtcat catgttgccc aacgcgatat aggtctggcg
    38581 gcaacaccag tcaaacaagg cgcgcgaatc gtccaaaatc ttgatgtcgt cggcttgaaa
    38641 cgcctgatac ctcgccaaag attttgccat ggcatccggt tcggtaacgc cgcgccgttt
    38701 gaggctttcc aacataaacg ggctgtcgaa gcgggcattt ttctttgcca aaaacaccac
    38761 caaatgactg gcggtatcca aagcatccgc cataccccaa gaaaacggct tgattgcctg
    38821 tcggatttcg gggttttgaa ccacaacaaa ctgccaaggc tccgaaccga ccgaactggg
    38881 cgacaaacgc ccgagttcta aaataaactg aaaatcctcg gcactgattt tacgtgccgc
    38941 atcgtaatgc cggcatgatt tgcggttttt aaatgcggat agaacctgct ctttgcttaa
    39001 tactgccatc gcacatcctt tcattacttg tattgcaaat tgatttcaaa ccgatacggc
    39061 ggcagattcg ccaataacgc ccaaacgcct ttcagacggc atttggttca tcgcaaacac
    39121 gcctacgggt aggcgatata ggcataagcc gaatggttgt ggatagactc gaaattctcg
    39181 ctctcgacga cgaaactctt gatgcgtttg tcggcgatca gcgaagtagc gacatcgcgc
    39241 accatatctt ccacgaattt cgggttttcg taggcctttt cggtaacgta tttttcatcg
    39301 gggcgtttga gcaggccgta gagttggcag ctcgcctgcg cctccacata atcgatgact
    39361 tcctcgatac cgacttcggc atcggcagtc aggctgacgg taacgtgcga acgctggttg
    39421 tgcgcgccgt attgggaaat ttctttggaa cacgggcaaa gcgaggttac gggaatcatg
    39481 accttcatac tgtggccgta tgcaccgtct ttcatttcgc ccgtgaggca gacatcataa
    39541 tccagtaaag actgaatacc ggaaaccggg gcggttttct tgcggaaaaa tgggaaagaa
    39601 acgctgattt taccggcgcg ggaatccaaa agcgcgacca tttcggcagt cagcctatgc
    39661 aattgtgcga aatccaaggc ttcagaatat tgctccatca atgcgacaaa gcgcgacata
    39721 tgcgtaccct tctgctcggc gggcaggtaa accgtcatgg tcagacgggc aatggtggac
    39781 tggatgcctt ctgcagtttg cagggtaatc ggaaagcgca ggtctttgat accgacctga
    39841 ttgatcggca gattgcgcaa atctcggctg gattgcacgt ctgcaatagt gttcatgagt
    39901 tgtttttcct taatattcat gtaattgttg agatgtgtgc atggtatggc gtttccgccg
    39961 gggaatatac cgtcaatctg caaatgcgag attatatcac tcgcttttag cagctgcatt
    40021 gattgtttct atttttcaga tagtgataaa atcttcgatt attcacatct acataggcag
    40081 agaccaaatg aaattcgcca cgaaagccat tcattccagc tacgattgcg acgaacacaa
    40141 ccgcgcgctg atgccgccga tttatcaaaa cagtatgttt gcgttgcacg agattggcga
    40201 aaatgtgcct taccgttatt cgcgcctgag caacccgacc cgtcagattt tagaagacac
    40261 cgttgccgat ttggaacacg gtgcggcagg ttttgcgttt tccagcggta tggcgggaat
    40321 tgatgccgta tggcgcactt tcctgcgccc gggcgatacc attgtcgccg tcgccgatat
    40381 ttacggcggc gcttatgatt tattggtcga tgtttatcaa aaatgggggg tgaacgttgt
    40441 ttttgccgat ttaggcaatc cggataattt ggacgaactg cttaaagcgc acaaggtcaa
    40501 actggtttgg ctggaaacgc cgtccaatcc acttttacgc ttggtagaca tcaaagtcct
    40561 tgccgcaaaa gccaaagcag ccggtgcgct ggtcggtatc gacaacactt ttgccacgcc
    40621 gtatctgcaa cagccgttgg atatgggttg cgattttgta ttccattccg ctaccaaata
    40681 tttgtgcggc cattccgacg tgttgatggg catcgtcgtt gccaaaacca aagaactggc
    40741 gcagcctttg cacgatatga tggtgcatac cggcgcggtt gccggcccgc tggactgctg
    40801 gctggtgttg cgcggcatca aaacactggc tctgcgcatg aacgcccatt gccaaaacgc
    40861 actcgaaatc gcgcgccgtt tggaagccca tcctgccatt gaaaaagtgt tccatcccgg
    40921 cctgccgtct cacgaacatt acgaactggc gaaaacacaa atgcccaaag gcatcggcgg
    40981 cgtggttacg gtttatctca aaaacgacac gcgtgaagcg gcaaacagcg tgattaaaaa
    41041 catgaaactg gtcaaaatgg cttccagcct cggcggtgtg gaaagtttgg tcaaccattg
    41101 ctattcccag tcccacagcg gcgtaccgca tgatgtgaaa atggaaatgg gcatcaaagt
    41161 cggtctgctg cgtttctcca tcggcatcga agacgcggac gatatttgga acgatatttc
    41221 cgccgcactc gatacgactt tgtaaactgt aaaaatgccg tctgaaacca tggtttcaga
    41281 cggcatttca attaacccgg ccgaaaatca acgcttcaac atctttgccg cctcaatagc
    41341 gtaataggtc aaaatcccgt ccgcacccgc acgtttgaat gccagcaggc tttccaaaac
    41401 cactttgccg ccgtccagcc agccgttggc aatcgctgcc tgcaacatcg cgtattctcc
    41461 cgaaacctga taggcataag tcggtacgcc gaactcgtcc ttcacgcggc ggacaacgtc
    41521 caaatacggt aaaccgggct tcaccatcac catatccgca ccttcccgaa tatcgagcgc
    41581 cacttcattc agcgcctcat cggtatttgc cggatccatc tggtaggttt tcttgtccgc
    41641 cttgcccaaa ttgcccgaac tgcccaccgc atcgcggaac ggcccgtaaa acgccgaagc
    41701 atatttggca gaatacgcca taatccgcgt atggatatgc ccggcatcct ccaacgcctc
    41761 gcgaatcgca ccgatacgcc cgtccatcat atcggaaggg gcaaccacct gcgcgcccgc
    41821 ttcagcgtgg cacaaagcct gcttgaccaa aacctctacg gtttcatcgt tcatcacata
    41881 accgttttcg tccgtcagcc cgtcctgacc gtgaaccgta taaggatcga gcgcgacatc
    41941 cgtcataatg cccagttcgg gaaacctctc gcgcaaggcg cggacagttg acggcacgag
    42001 tccttcggga ttgtacgcct cctgcgcacg ctcggttttg tttgccgtaa ccacggggaa
    42061 cagtgccaac atcggaatac cgagctttac cgcctcttcc gctgtaaaca gcagcctgtc
    42121 caaactttga cgcttcacac ccggcataga aggcacatcc tcctcgcgcg ccgacccctc
    42181 caatacgaac accggataaa tcaaatcatc ggcggtcagc gtgtgttcgc gcatcaggcg
    42241 gcgtgaaaaa tcgtccctgc gcatacggcg catacgcgaa gccggaacat tgcggtaagg
    42301 aaactgcata agccctccaa tcatcctgtc aacaattcaa accatacgga agccgccgca
    42361 acttgcagac agcatcgggc ggcaatcaaa atccgttttc accggaaagg gtttcggccc
    42421 gccgaaatgc cgcgccgaag gacggcgtcc cgcccattaa agaacaatag caaaacaaca
    42481 gcttcaggct gtcagccttc gatattctta cgccacaata ccaaaagttt cccgatatgc
    42541 tgaacaggtt gcgcgtcaac cgcctcacat aaggcagtgc agatttcgat acgttcggca
    42601 cggtcgtcgc cgaatacgcg cactttaatc agctcatgcg ccgtcaatgc cgcatcggtt
    42661 tccttgatga ccgcgtccgt cagaccctgc tgaccgacca tcacaacagg atggagatgg
    42721 tgcgcgcgcg ctttcagttc caaaatttct ttggtgttca atttggtatc cgtcattttc
    42781 ctactgcttg aataaaaaga ataatgcggc attgtacgcg atttggcacg acatcggcaa
    42841 aattaacgta caatacgcgg tttccattcc gacccgcgaa ccactatggc tgtacgttcc
    42901 aaatcctcaa aagcgtggct gcacgaacac gtcaacgacc actacgtcca tatggcgcaa
    42961 aaagacggct accgcgcccg tgccgcatac aaacttttgg aaatcaacga aaaagacaaa
    43021 ttaatcaaac ccggcacggt acttgccgac ttgggcagcg caccgggaag ctggtcgcag
    43081 gttgccgcca agctgacggg tacttccgga gcagttttcg ccttggacat cctgcctatg
    43141 gaagccatag ggggcgtctc cttcattcag ggcgacttcc gcgagaacga cgtactggca
    43201 caattcgaaa ccttgttgga caaccgcccg ctcgaccttg taatttgcga tatggcgccc
    43261 aatatgtcgg gaaacgccgt aagcgatcag gcacgcagct tttatctgtg cgaactggct
    43321 ttagacttcg cctcgcaaca cctgaaaacc ggcggcagct ttttggtcaa agtctttcag
    43381 ggtgcaggct atcaggaata catggcagcc atgcgcgaaa ttttcggcac ggtgcagacg
    43441 cgcaaacccg aagcctcgcg caatcgctcc agtgagattt atttattggg caaaaataaa
    43501 cgctgacaat acagacggcg tgctttacaa tcatttccgt tttacacctc aattatggag
    43561 ccttgctaag tggggaacac ctttaaatca atccttgtct gggtcgcctt gggtatcggc
    43621 ctgatggctg cgttcaacgc tttagacggt aaaaaagaag acaacgggca aatcgaatat
    43681 tctcagttca tccaacaggt caacaacggc gaagtatccg gcgtcaacat cgaaggatcc
    43741 gtcgtcagcg gctacctgat taagggcgag cgcaccgaca aaagcacctt cttcaccaac
    43801 gcgcctttgg acgacaacct gattaaaaca ctgctcgaca aaaacgtccg tgtaaaagta
    43861 acgccggaag aaaaaccgag cgcgctggct gccctgtttt acagcctgct gcccgtcctg
    43921 ctgctgattg gcgcgtggtt ctactttatg cgtatgcaga cgggcggcgg cggaaaaggc
    43981 ggcgcattct cattcggcaa aagccgcgcc cgcctactgg acaaagatgc caacaaagtt
    44041 acctttgccg atgtcgccgg ctgcgacgaa gccaaagaag aagtgcagga aatcgtcgat
    44101 tacctcaaag cgccgaaccg ctatcaaagc ctgggcgggc gcgtgccgcg cggcatcctg
    44161 ctggcgggca gcccgggtac gggtaagacg cttttggcga aagcgattgc aggcgaagcc
    44221 ggcgtgccgt tcttcagcat ttcaggttcc gactttgtcg aaatgttcgt cggtgtcggt
    44281 gcaagccgcg tccgcgatat gttcgagcag gcgaagaaaa acgccccctg catcatcttt
    44341 atcgacgaga ttgacgcagt cggccgccaa cgcggcgcag gtttgggcgg cggtaatgat
    44401 gagcgcgagc aaacattaaa ccaattgttg gttgaaatgg acggttttga gagcaatcag
    44461 actgtaattg tgattgcggc aaccaaccgc cccgacgtac tcgatcctgc gctgcaacgc
    44521 cccggccgtt tcgaccgcca agtggttgtc cccctgccgg acatccgggg gcgcgaacag
    44581 attttgaacg tccactctaa aaaagtgcct ttggacaaat ctgtggattt attgtccctc
    44641 gcgcgcggca cgccgggttt ttccggcgcg gatttggcga acttggtcaa cgaagccgcc
    44701 ctgtttgccg gccgccgcaa taaagtcaaa gtcgatcaga gcgattttga agacgccaaa
    44761 gacaaaatct atatgggtcc ggaacgccgc agtatggtga tgcacgaaga cgaaaaacgt
    44821 gcgacggcgt atcacgaatc cggacacgcg attgttgccg aaagcctgcc ctttaccgac
    44881 cccgtccaca aagtaaccat tatgccgcgc ggacgtgcgc tgggtctgac ttggcagctt
    44941 ccggagcgcg accgcatcag tatgtataaa gatcagatgt tgagccagct ctccatcctg
    45001 ttcggcggac ggattgccga agacatcttc gtcggacgca tctccaccgg cgcatcaaac
    45061 gactttgaac gcgcaaccca aatggcgcgc gaaatggtaa cgcgctacgg catgagcgac
    45121 aaaatgggcg tgatggttta tgcggaaaac gaaggcgaag tcttcttggg acgcagcgta
    45181 acccgttcgc aaaacatttc cgagaaaacc cagcaggaca tcgatgcgga aatccgccgg
    45241 attttggacg agcagtatca ggttgcctac aaaatcctcg atgaaaaccg cgacaagatg
    45301 gaaacgatgt gcaaagccct gatggaatgg gaaaccatcg accgcgatca ggtactggaa
    45361 atcatggcgg gcaaacaacc cagcccgccc aaagattaca gccacaacct gcgcgagaat
    45421 gcggacgcgg cggaagataa cgcgccgcac gctccgactc gggaagaaac cgaagcacct
    45481 gccccggcag acaccgcttc gacagagtcc gagcagcagc ctgaaaacaa ggcttaactt
    45541 cccgaacaaa cggcagcccg caagctgccg ttttcccatc ctgttttcag acggcatttt
    45601 ccgctcaatg ccgtctgaaa cgccaaagcc gtatcggttt ttgcgaatct gcgatatggc
    45661 ggtttaaatt gaaatcagaa cggggcaagg ctgtaccggt ttaaaattaa accgctataa
    45721 taccgccctt cgaatacacg cccgcattgg tgatgcccgt gaaaaaagtt gaaaaaaaca
    45781 tcttggtgct gcacggcgcg gacaaaatgt tcgagctggt cgataaggtt gaggattacc
    45841 cgaactttct gccgtggtac agcaaaaccg aggtcatcgg acgcaacggc aacgaactga
    45901 aggcgcggct gtttatggac tatatgcacg ttcgccaatc attcgccacg cacaaccgca
    45961 acattccggg cagggaaatc cgtatggaac tgctcgaagg tccgttcaaa accttacgtg
    46021 gaacgtggaa atttatcgat ttgggcgacg atatgtgcaa aatcgaattc aatttggaat
    46081 acgatttttc caatgccgtt ttgtccgcct taatttcccc cgtcttcaac cacctttccg
    46141 ccacgctggt cgaagcgttc gtcaaagagg cagaccgccg ttatgcttga aattgagatt
    46201 gtgtacgggc tgcccgaccg acaggttttg aaaaccatgc agcttgccga gggaacaacc
    46261 gtccgcgccg ccgcactgca aagcggtttg gacggcatat ttgaagattt aaacctgcat
    46321 tccgcgcctt tgggcatttt cggcaaagcc gtcaaagacg acacgccgct gcgcgacggc
    46381 gaccgcatcg aagtgtaccg cccgctgttg atcgacccca aagaagcgcg ccgcaaacgc
    46441 gttcaaaatc aagaagaata accatgccgt ctgaagcctt cagacggcat tcggcagaaa
    46501 cacggaaaat atcatgtcaa acacaatcaa aatggttgtc ggcttgggca acccgggcaa
    46561 agaatacgaa cagacccgcc ataatgcagg cttttggttc ctcgacgaac tggcttggaa
    46621 gtggaaggct tcgtttaaag aagaaaaaaa attcttcggc gaagtcgccc gtgctaccct
    46681 gcccgacggc gatgtctggc tgctcaagcc gaccacgttc atgaaccgtt ccggacaggc
    46741 agttgccgcc cttgcgcagt tttataaaat caaacccgaa gaaatcctcg tcgtccacga
    46801 cgaactcgac attccctgcg gacggatcaa attcaaactc ggcggcggca acggtggaca
    46861 caacggcttg aaagacattc aggcacggct cggcacggcc gactattacc gcctgcgcct
    46921 cggtatcgac cacccgggcg accgcaacct cgtcgtcggc tatgtcctga acaaacccag
    46981 tacggaacac cgccgacaga ttgacgatgc cgtcgccaaa tccctgcagg ccatacccga
    47041 catccttgcc ggcaaatggg aagaggcaac ccgcttcctg cacagcaaat gacccgatgc
    47101 cgtctgaagc cctttcagac ggcatgttcc cgatttccat atccgaacag tcatgaccga
    47161 actcaagcag cttatccaaa ccgaatccat cctcgtcatc gaagaaaccc tcgatttcct
    47221 gctgtacgaa tgcagcatcg acgacgcacc atccgccgaa gaagtggcac aatggcgcga
    47281 catacttgcc gcacgcggcg gcaaattcct gcgcctgtcc aaaatctgcc aaacgtggct
    47341 ggacgaggag gcggcatgaa gctgccgcgc aaccgcttca gcctgctttc cgcattgtgg
    47401 tttgccggcg gcatctattc gctgctcttc aaagctgccg acaccgcgcc gccgccgttt
    47461 ccgcatttcg acaaagcagc acaccttgcc ctgtttttcg cacaaatctg gcttttgacc
    47521 aaagcattca aaaccggaaa acttcccatc ccctaccgca gcctgatggt ctttgccctc
    47581 tgtttcgccc tcttcagcga atgcgcgcag gcatggttta ccgcaacgag aaccggcagt
    47641 ttgggcgatg ttcttgccga tatggcaggt acggttctcg cactctttgc cgcccgcgcc
    47701 gccgaccgcc cggactgaat cggtttattt cccccaaaca ggatgcacct cttcccgata
    47761 catcctattt ttccccttac atatcaatcc catctcataa acaaaaccga caaatcgttt
    47821 acaatatatt tacactacat cagattacaa atatactcga accagttgca gaatcggcgg
    47881 ttccacaaac cgtttcggat tgcacgacac aaaagaaaaa ccgattttat tgccttaaag
    47941 gagtattcat gaacctgcat gcaaaggaca aaacccagca tcccgaaaac gtcgagctgc
    48001 tcagtgcgca gaagccgatt accgacttta agggcctgct gaccaccatt atttccgccg
    48061 tcgtctgttt cggcatttac cacatcctgc cttacagccc cgatgccaat aaaggtatcg
    48121 cgttgctgat tttcgttgcc gcactttggt ttaccgaagc cgtccacatt accgttaccg
    48181 cgctgatgat ccctatcctc gccgtcgtac tcggtttccc cgacatggac atcaaaaagg
    48241 cgatggctga tttttccaac ccgattatct acattttttt cggcggcttc gcgcttgcca
    48301 ccgccctgca tatgcagcgg ctggaccgta aaatcgccgt cgccctgctg cggctgtcgc
    48361 gcggcagtat gaaggccgcc gtgctgatgt tgttcctcgt taccgccttt ctgtccatgt
    48421 ggatcagcaa caccgccacc gccgcgatga tgctgcctct ggcaatgggt atgctgagcc
    48481 acctcgaccg ggaaaaagaa cgcaaaacct acgtcttcgt cctgctcggc atcgcctatt
    48541 gcgccagcat cggcggcttg ggcacgctcg tcggctcgcc gcccaacctg attgccgcca
    48601 aagccctaaa tctggacttc gtcggctgga tgaagctcgg cctgccgatg atgctgttga
    48661 ttctgccctt gatgctgctc tccctgtacg tcatcctcaa acctaatttg aacgaacgcg
    48721 tggaaatcaa agccgaatcc atcccttgga cgctgcaccg cgtgatcgcg ctgttgattt
    48781 tccttgccac agccgccgcg tggatattca gctccaaaat caaaaccgcc ttcggcattt
    48841 ccaatcccga caccgttatc gccctgagtg ccgccgtcgc cgtcgtcgtc ttcggcgtgg
    48901 cgcaatggaa ggaagtcgcc cgcaataccg actggggcgt gttgatgctc ttcggcggcg
    48961 gcatcagcct gagcacgctg ttgaaaacat ccggcgcgtc cgaagccttg ggacagcagg
    49021 ttgccgccac cttttccggc gcgcccgcat ttttggtgat actcatcgtc gccgccttca
    49081 ttatttttct gaccgagttc accagcaaca ccgcctccgc cgcattgctt gtaccgattt
    49141 tctccggcat cgctatgcag atggggctgc ccgaacaagt cttggtattc gtcatcggca
    49201 tcggcgcatc ttgtgccttc atgctgccgg ttgccacacc gcctaacgcg attgtgttcg
    49261 gcacgggctt aatcaagcaa cgcgaaatga tgaatgtcgg catactgctg aacatcctct
    49321 gcgtagtatt ggttgctctg tgggcttatg ctgtactgat gtaaaccatc gacctaaaca
    49381 acaagaccgt ctgaaagaat atttttcaga cgaccttgaa gtttgtcgtt cagacacaat
    49441 ttgtcgaatc attcaaaacc ggattctaac gaaaggaaac ccatgattat cctgcacacc
    49501 aacaaaggcg acatcaaaat cgaactcgat ttcgacaaag cccccgttac cgccaaaaac
    49561 ttcgagcaat acgtcaaaga cggcttctac gacggcgtaa tcttccaccg cgtcatcaaa
    49621 ggcttcatga ttcaaggcgg cggcatggat gaaaacatga acgaaaaaga aacccgcgat
    49681 ccgattcaaa acgaagcgtc caacggcctg cccaacgata aatacaccat cgccatggca
    49741 cgtacttccg acccccattc cgctggcgcg caattcttca tcaacaccgc cgacaatgcc
    49801 ttcctcaacc accgctccaa agaaatgtac ggcagaaccg tcgtccaaga ctggggttat
    49861 gccgtattcg gcaaggtagt cggcggtttt gacgtggtcg atgccatcga aggcgtatcc
    49921 accaaacgcc acggctacca tgatgatgtg ccgacagaac ctgtcatcat cactaaagcc
    49981 gaagcggtat aaaccggcaa tccgaaagca gcctgcacaa aggctgcttt ttttgtttca
    50041 ggcggcataa aatccaagca catatgccgt ctgaacacct cccgcaagcc ttcggacaat
    50101 gttgcaacgt gttaaaatag cctcctttcc tgaaaccaca acaaggaaac catcatggca
    50161 aatattgccc gcgacatctt caaagcctac gacatccgag gcattgtcgg caaaaccctg
    50221 actgacgatg ccgcctatct catcggcagg gccatcgccg ccaaagccgc cgaaaaaggc
    50281 atgacccgca tcgcgctcgg acgcgacgga cgcttgagcg gtcccgaact gatggagcac
    50341 atccaacgcg gcctgaccga cagcggtatc ggcgtcctca atgtcggcat ggttgccacg
    50401 cccatgctct attttgccgc catcaacgaa tgcggcggca gcggcgtgat gattaccggc
    50461 agccacaatc cgcctgatta caacggtttc aaaatgatgc tcggcggcga cacgcttgcc
    50521 ggcgaagcca tccaagagct tttgtccatc attgaaaaag acggttttgc tgccgccggc
    50581 aaacaaggca gcgtaaccga aaaagacatc tccggcgaat accacaacca catcgtcgga
    50641 cacgtcaaac tcaaacgccc gatgaacatc gctatcgacg cgggcaacgg cgtgggcggc
    50701 gcatttgcag gcaaactcta ccgcgctttg aggtgcggcg ttaccgaact tttctgcgac
    50761 gtggacggca atttccccaa ccaccatccc gacccatcca aacccaaaaa cctgcaagat
    50821 ttgattgccg cgctgaaaaa cggcgatgcc gaaatcggct tggcgtttga cggcgatgcc
    50881 gaccgcttgg gcgtggttac caaagacggc aacattattt atcccgaccg ccaactgatg
    50941 ctgttcgcgc aagacgtgtt gagccgcaac cccggcgcga aagtcatctt cgacgtgaag
    51001 tccacccgcc tgcttgcccc gtggattaaa gaacacggcg gcgaagccat aatggaaaaa
    51061 accggccaca gcttcatcaa atcgaccatg aaaaaaaccg gcgcactcgt cgccggcgaa
    51121 atgagcggac acatcttctt caaagaacgc tggttcggat tcgacgacgg tctgtacgcc
    51181 ggcgcacgcc tcttggaaat cctgtctgcc tccgataatc cgtccgaagt gttaaacaac
    51241 ctgccgcaaa gcatttccac gcccgaactc aacatcgccc tgcccgaagg cagcaacggc
    51301 catcaggtta tcgacgaact cgccgccaaa gccgaatttg aaggcgcaac cgaaatcatc
    51361 accatcgacg gtcggcgcgt tgaatttccc gacggcttcg gtctgatgcg tacttccaat
    51421 accacgccga ttctggtgtt gcgttttgag gcggatacgc aagaagccat cgagcggatt
    51481 caaaaccaat tcaaagccgt catcgaaagc aatccgaatc taatctggcc tctgtaaaca
    51541 caggaaaaat gcttaatcaa atcttggtag attggatttg gcttaaaaat ctccgcgtcc
    51601 gttcgtgttt ggggtggtcg aacaactctt tcgggctgcc ctgctctacg ataacgccgc
    51661 cgtccatcac gacaacggtc gtggcaactt ccagcgcgaa cttgatttcg tgggtaacga
    51721 cgaccatcgt ccaaccttcc cgcgccaatt ccttcatggc gttcaacacg tcttgcacca
    51781 actcggggtc aagcgcggaa gtgggttcgt caaacaacat cagctcgggc tgaatcgcca
    51841 atgctcgggc aatgccgacg cgctgctgct gaccgccgga aagctggtag ggatacaaat
    51901 ccactttgtc gcccaagccg actttttcca gcagtttcag agcctcttcg cgcgcttggg
    51961 cggcaggctt gccctgtacg gcaaccggtc cttccatcac gttttccaag gcggttttgt
    52021 gcggaaagag gttgtattgt tgaaacacca tgcctgattt gcggcgcagt gccaaaatat
    52081 cgtgtttgct tggtttttta gaaaaatcga ttttcagcgg tcgctcgttg tcgaactcga
    52141 tttgtccgtc ttcgggcatt tccaacgcgt ttaggcatcg cagaaacgtc gttttgcctg
    52201 agccggaagg cccgaggatg acgaccacct gccctttgca cacatccaaa ttgatgccgc
    52261 gcaaaatggt atttttgccg aaggtcttat ggatattgcg gattttaatc atgacaactc
    52321 cttatttggc gacgtagcgg tcgaaacgtt tttccaaacg cgcctgaatc aggaacagca
    52381 ctttacaaaa acaccagtaa accaaagcgg cttcgatata gacgggcaaa aagtcataag
    52441 tgcggtttgc cgtttcctgc gcgacgcgga ataattccgt taccgtcacg actgccgcga
    52501 gcgaggtgtt tttaaacaaa ccgataaact cgttgctcaa aggcggcacg gcaacgcgga
    52561 atgcctgcgg cgcaacgatg cggcggaacg tctgcatata ggtcatgccg atggagaaac
    52621 ctgcttccca ttggcctttc ggtacggaca aaattgccgc gcgtatggtt tcggaagcat
    52681 atgcgccgac attgagcgaa aagccgatga tggcggcagg aatcgggtcg atatagatgc
    52741 cgacggaagg cagcccgtaa aacacaatca caagctgaac caacagcggc gtaccgcgaa
    52801 tgacggaaat ataaaattcc accaatttca gcaggatttt ccgcacgatg ccgccggcgg
    52861 gcataatccg caccaaagcc acggctaccg caatcatcat accgataacg aaagaagctg
    52921 ccgccaaagg cagagagacc gcgaagccgg ctttgaccat aggcaaaaac gcgctgacaa
    52981 tcatatcggc gcgtgtttcc gtcataaacg gcagcgaagc aaggaaatta ttgaacactg
    53041 atgtcttttc cgaagaattg ttcgcccagt tttttcagcg taccgtcggc tttcagctcg
    53101 ttgattgccg tactgaattt cgccacggct tcgtcattgc ccttgttgac aatcaggccg
    53161 gaaccgactt tttcatcggc aggtgcggac caaacgattt tcacgcccgc attcgggttt
    53221 ttcttcagat agtccaaaac cgccaattcg tcgttcaggg ttgcatcggc acgtttttgt
    53281 tcaatcaggg tcagcgattg cgccaaaccg tccactgcca ccaaatctgc gcctgcagct
    53341 ttggcttttt cgccgtagtt gctggtcagg gattgtgcgg ttttcacgcc tttgatgtcg
    53401 tcgatagatt tgatgttgct gtcgttacgg gcaaccaata cggcaccgct ccagctgtaa
    53461 ggatcggatt tgtcgaatgt cgcttggcgt tcggggctgg tcagaccgac ttggtttgcc
    53521 accacgtcga aacgccccgc cttcaaaccc gccatcatcg aatcccattg cgtttcttta
    53581 aactcgactt tcacgcccag tttttccgcc acggcgcggg ttacttccac atcgtaaccg
    53641 gtcagtttgc cgtccttgtc gtggtaggta aacggtgcgt aagtgccttc cgtgccgacg
    53701 gtaaccgtgc ctttattgtt gatgcgctcg attaaagaac cggaaactgc cgattgtgca
    53761 ggcgcggaag atgctccgct gccgctttcc gaaccgccgc aggccgccaa aaccaatgcg
    53821 gcaatgccgc cgagtacgaa ttttttcaac atcattctct cctgtaaaaa gtgtgcaagt
    53881 gcggcattct aagtgaaatt gcggcaaaca caaagaatac tttttttata tggttattcc
    53941 aaatcggaat ggaaaagata tgccgaagcc gccggaagcg gaaataaagg gggcgggaaa
    54001 ggcggcgggc ggcacgaaac cgaaaagcgg catcaggtat gcaaacggcg gattcacgca
    54061 cgccgttccg gcaaaaatcc gcctgccgat tcggtgctat aatccatccg aaacagatga
    54121 caccaaaaag gaacatctca tgaacacgcc ccaatccgcc atcattcccg accacgccca
    54181 agccggcatc tttatcgaag ccgacttcgc cgccaaccgc ctcaacgata ttaaagccgc
    54241 ctgccgcgct tcgctcgacg cgttatccgc cttgaaaacc cgctttccgg acgatatttt
    54301 gggtctgacc atcgccttcg gcagcaaagc ctgggcaaca ttcggacata cggacgaagg
    54361 cagtgaaatc aaacccttct ccgaaatggg caacgggctt gcgccgtcca cgcagcacga
    54421 tatgtccatc cacatccaat ctttccgcca aaacgccgcc tacgcgcttg cccaatccgt
    54481 tttgggcgcg ttcggcgaca gcatatgcgt cgcgtccgaa gaacacggtt tgcgcctgta
    54541 tcaggatcgc gggctggacg gtttcgtcga cggcacggaa aacccgcagg gcgatgaaaa
    54601 tgtccgcgaa gtcgccatca tccccgaagg aaaacccgat gcgggcggca gctatgtcct
    54661 gctgcaaaaa tacctgcacg atttgaaaaa atgggatgcc gtccccgtcg ccgaacagga
    54721 ggcctcggtc ggacgcggca aggaaaccga cgacgaattc ggccacgatg tccgcctgcc
    54781 cgattcgcac ctcggccgcg tcaacctgaa agaaaacgga gtcggcctga aaatcgtccg
    54841 ccgcagcctg cccttcggca aaatcagcgg cgaacacggt ttgatgttta ccgcatactg
    54901 ccgcacgctg cacaatatcg aagcgcaatt attgagtatg ttcggcgaca cggatggcaa
    54961 aaccgacctg ctgctccggc acctctccgc cgccgtttcg ggcggatact actacgcgcc
    55021 gtctgtcgaa cgcctgcaaa acctctaagc cagacgatgc cgtctgaaac catttttcag
    55081 acggcatttt cccgttttat agtggattaa caaaaaccag tacggcgttg cctcgcctta
    55141 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
    55201 tgcactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taccgtccaa
    55261 ccgttttccg agcgcgcact atgtctgcaa gcatccaagc attcgacccg ctaaccgtcc
    55321 ccatttccgg caccaacctg attgaggcct ctgccggcac cggcaaaacc tacggcattg
    55381 ccgccctgtt tacacgcctg atcgtattag aacaaaaaaa cgtcgaacgc gtattggtcg
    55441 taaccttcac caaagccgcc accgccgagc tgaaaacacg cctgcgcggg cgtttggacg
    55501 atgtgttaca agttttagaa agcaaaggaa ttgccaaact tggagacgac acgctttcag
    55561 acggcattgc cgcctactgc gccgaacacc acgaaggcga caccttcctg cccgaactct
    55621 taaaacaggc tttgcaaaaa gagggccgga cgcgtctgat tgtccgcctc aaggccgcca
    55681 tcgggcaatt cgacaacgcc gccatctata ccatccacgg cttctgccag cgcatcctgc
    55741 gcgactacgc cttcctgtgc caagcaccgt tcgatgtcga aatgaccgaa gaagacggcg
    55801 accgcctgct tatcccggcg caagattttt ggcgggaacg cgtcagcaat gatccggtgc
    55861 ttgccgcatt ggcgcttaaa cgcaaagctg tgccgcaaac cgtccttgcc caaatttcgc
    55921 gctatctttc ccgcccgtac ctgaatttcc gccgtccgca ggcggatttg aaacaggcgc
    55981 agcgcaacgc cgaaacctca tggcaaacca tctgccgcct gctgccagaa ctggaagccg
    56041 gcttttggcg cattcatccc gacctcaacg gcaacagtta ccgcaaaaac agcttcggca
    56101 acctttttaa agaactggca caaaaatccg ccgccggaca actgcccttt ctggacaaag
    56161 acacacacga cagactcttg aaacttgcat ccgacaaact cgaagccgga ctgaaaaaag
    56221 gcaaaacgcc cgatgcggca gtatttgccg aattgcagaa actggcagac ttcgggcgcg
    56281 atttgaacgc actcgaagaa gcggaagaag caacaatgat ccggctgcaa ctggatttaa
    56341 tcgaatacct caaccgcagc cttgccgaga tgaaaaaatc gcgccgcgaa cgcggtttcg
    56401 acgacctgct gctcgatgtc cacaccgcgc tgaccgacaa tccgcacgcc gacactctcg
    56461 cccgcgccgt tgccgaaaac tgggaaaccg cgctgatcga cgagttccaa gacaccgacc
    56521 cgctgcaata cgaaatcttc caaaagattt tcatcgccca aaaccgaccg ctgttcctcg
    56581 tcggcgatcc caaacaggcg atttacagct ttcgcggagc ggacatttac gcctaccttc
    56641 aggcggcgga agacgcgcgg caccgctaca cgctcgccac caactaccgc agccacgccg
    56701 cgcttatcgg cagcataggc gcgctgttcc gcctcaaaga acgcccgttc gttttggaaa
    56761 acatcggcta ttcggaagtc ggtgcggcgc gtgccgaaag caggctgtcc cccgaacgtc
    56821 ctgccgtaca ggttcgttgg ctgcacgaaa acgacaatga aaaagccaac aaagacgttt
    56881 tgcgccgccg tgccgccgac tattgcgccg acgaaatcgc ccacgcgctc aacgaagccg
    56941 ccagaggccg tctgaatttc aaaggctgcc cgttgcagtc gggcgatatt gccgtgctgg
    57001 tacgcacgca caacgaggcg gtgatggttt ccgccgccct gaaaaaacgg caggtgcaaa
    57061 gcgtcctgct ttcgcgcgaa tccgtgttcg cctcgcccga agccgccgcc ctgtccgcac
    57121 tcatcggctt ctggctcgaa ccgcgccgcg ccggaacgct gcgttttgtc ctgacaagca
    57181 gcatattcgg ttatgacgcg cagcaattgc acgacttcaa ccaaaacgaa agcgagattt
    57241 tgcattgggc ggaatctgcc cgaaccgcac tcgacaactg gaataaatac ggcattttcg
    57301 ccgctatgca gcaattttcc caaacacacg gcatcgaaac gcgcctctta agccggaaca
    57361 acgggcgcag tctgaccaat tatttccaac tgctcgaact gcttgccgcc gaagacgcgc
    57421 aaaaccgcaa ccccgccgcg ctgcacaaat ggctgggcga ccaaatcagc cttgccgaaa
    57481 acaacagcgg cgacaaccgc gccatccgtc tggaaagcga cgaagatttg gtcaaaatcg
    57541 ttaccatgca cgcctcgaaa ggtttgcagt atccgctggt gtactgcccg tttgcgtggg
    57601 acgcgcaaga taccggaccg tccgactggc aaatcctcca ccaaggcgca aaccgaaccg
    57661 aactgttggc aaaggcgcaa ctgtcggaag acgaacagaa acaatacgcc gatgaagaaa
    57721 tggcggaacg cctgcgcctg ctttatgtcg cgctgacgcg tgccgaggaa cagctcaaca
    57781 tctacgccgc gtactccacc aatactgccg acaaccccct cgcctacctg attgaaggct
    57841 cgccccaaga cagccgtgaa accgtccgcc gtgcctatgc gtgtgaaaaa gacggcatcg
    57901 cgatgctcaa acgcaactgg cggcgcgtgg cggacaacgc cccttccggc acaaatttcg
    57961 ccttcaccga aaatgcgccg ccccctgccg cctatcgcag caacgccggt caaaccgccg
    58021 aatttgccgc aaacagcatt cccgaacgcg gattccgatt tgtccgccac actagcttta
    58081 ccgccttaag ccgccatacc caaacgcccg acggcggcga agaagatgcc tgcccgtcct
    58141 tggatgccgc cgaaacctcg gtgccggcga tgccgtctga aacgccgacg gcttcagacg
    58201 gcatatcgat acacgatttt ccgaaaggca cgcaggcggg gctgtgcctg cacgaaattc
    58261 ttgaagattt caaattcgga caagcagccg ccggacagga aaccctcatt gccgacaagc
    58321 tgaaaaaata cggttttgaa gaaatatggc tgcccgccgt tgccgaaatg gcggaagcct
    58381 gccgcaaaac gccgctgacg ggggcatacg acctgtccga catctcaccc gagtgccgct
    58441 gtcccgaaat gggctttacc ctccacaccg aagacttcag cctcaaacgc ctgcgcgact
    58501 ggtttgcccg cgacgacatc aggctgcccg aagtctgccg tgccgctgcc gaaacgctcg
    58561 acttccacac cgtcaacggc tttttaaacg gctttgtcga tatggtctgc caagaccccg
    58621 acggcaatat ctgcgtcatc gactacaaat caaaccacct cggtacggac gcatccgcct
    58681 acacgcaaca ggcaatggac gaagccgtcg cacaccacca ctattacctt caggcactga
    58741 tttacgccgt tgccgccgca cgctacttca agctgcgcgg acaaccgccc gccgccgttt
    58801 ccgtccgcta cctgtttttg cgcggattgg atggcaaagg cggcggcgtt tggcgttggg
    58861 acatagatgc cgccgctttg gaacagataa aataacttcc cgtcctttcg gacaacaacc
    58921 gcaggaaaac cctatgaata tcaaacacct gattaccgcc gcactcattg cctcagccgc
    58981 ctttgccgcg caggcagccc cgcaaaaacc cgtatccgcc gcccaaaccg cgcaacatcc
    59041 ggccgtttgg atcgatgtcc gttccgaaca ggaatttagc gaagggcatt tgcacaacgc
    59101 ggtcaacatc cccgtcgacc aaatcgtccg ccgcatacac gaagccgcgc ccgacaaaga
    59161 cacgccggtc aacctctact gccgcagcgg acggcgtgcc aaagccgccc ttcaggagct
    59221 gaaaaaagca ggttatacaa atgttgccaa tcacggcggt tatgaagacc tgctcaaaaa
    59281 agggatgaaa tgacgcaatg ccgtctgaaa ggacaggctt tcagacggca tacggcgttc
    59341 cggcacgcgg cgcaacatcg ggcgcggcgc gcgccgcccc gtccgaagcc tgataaacaa
    59401 accgataagg aacaatgatg tttcacgaca cataaagcac cgccctatgt gttgccctga
    59461 tttggaaagg gttacacccc tcccgaataa agtctgattc taccgtttca accaaaaagg
    59521 aaaaaggaaa tacgatgaaa cacatactcc ccctgattgc cgcatccgca ctctgcattt
    59581 caaccgcttc ggcacatcct gccagcgaac cgtccaccca aaacgaaacc gctatgacca
    59641 cgcataccct cacctcaaaa tacagttttg acgaaaccgt cagtcgcctt gaaaccgcca
    59701 taaaaagcaa agggatggac atttttgccg tcatcgacca tcaggaagcc gcccgccgaa
    59761 acggcttaac gatgcagccg gcaaaagtca tcgtcttcgg cacgcccaaa gccggcacgc
    59821 cgctgatggt caaagacccc gccttcgccc tgcaactgcc cctacgcgtc ctcgttaccg
    59881 aaacggacgg caaagtacgc gccgcctata ccgatacgcg cgccctcatc gccggcagcc
    59941 gcatcggttt cgacgaagtg gcaaacactt tggcaaacgc cgaaaaactg atacaaaaaa
    60001 ccgtaggcga ataagcccgt cgccgacata tggcggatgg aaatcaaatg cacacgcccc
    60061 tcattcccac gaagtcggga atccaatcgg ttgggttacc gtgattttca atcattgccg
    60121 aaacggacga ttccggattc ccacccttgc ggggatgacg ggcgtgtgtg tttttgccac
    60181 atcacaggca actttgccgc aaatcttatc tgtcgccggt atgccttctt gtttcaatcc
    60241 gccacaaacc aaatgccgtc tgaaacccga tttcagacgg catttccgtt tattccctgc
    60301 tgtcgcggca aatatcgatg gcttcctgca aactcgaaac gccgtagatt ttcaggttcg
    60361 gaaactcttt ggcgttgcgc ggcatattgg ctttggggac gatggcgcgt ttgaagccga
    60421 gtttttccgc ttctttgagc cgctcttgcc cgcgtgcgac ggggcggact tcgccgctta
    60481 agccgatttc gccgaaaacc acggtttttt caggcatagg gcggttgcgg aagctggaaa
    60541 gcatcgcgag gatgaccgcc aaatccgccg ccggttcgcc gattttcacg ccgccgacgg
    60601 cgttgaggaa cacatcctga tcgaaacagg cgatgccgcc gtggcggttt aacacggcaa
    60661 gcagcatcgc aagacggttt tgttccagtc cgacggtgag gcgtttgggc gtgaagccgt
    60721 gcgcgtcatc gaccaatgcc tgaatttcga ccaaaagcgg gcggctgcct tcctgtgtaa
    60781 ccaaaacgca cgagccgggc gtatcgtcgc ggtagctggc gaggaagatg gcggacgggt
    60841 tggacacacc tttcaaaccg ttttccgtca tcgcgaacac gcccagttcg tttgccgcgc
    60901 cgaagcggtt tttgatggcg cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat
    60961 acagcacggt atcaaccatg tgttccagca cgcgcgggcc ggcaatcgcg ccgtctttgg
    61021 tcacgtgtcc gaccagtatc atggcgatgc ccatctgttt cgccatgcgc gtcagttggg
    61081 cggcacactc gcgcacctgc gacacggagc cgggggcgga cgtgatttgg tcggaataca
    61141 tggtttggat agagtcgatg acgacaactt cgggctgatg ctgtttcaag gccgcctgaa
    61201 tcgcttccat gcggatttcg gcaagaaggt ttacgccgtc ggtcggcagt tccaaacgct
    61261 gcgcgcgcag ggcgacttgt tgggcggatt cttcgccgga aacgtatagc actttacggc
    61321 tttgcgccat tttggcgatg gtttgcaaca gcagcgtgga tttgccgatg ccggggtcgc
    61381 cgccgagcag gatgaccgca ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc
    61441 ccataccggt cggattgcgc ggcacttcgg tggcggtaac ggcggagagg gattggacgg
    61501 tcgaagtgtc cgccgcccaa gattggaaac gggcgttttt cggctcgggc gcggcaaggc
    61561 tttcctgaag cgtgttccac tcgccgcaat gcgggcattt gccttgccat ttcggggagg
    61621 tgccgccgca ttcggtgcat tggtaaaggg ttttaagcgt ttttgccatc ggttttccct
    61681 gcatctgaaa acaaaaatgc cgtctgaaaa tagggacggt tgggattgcg ccaatacgac
    61741 aacgccgccg ccgtcatgta ctatccatac acggcggata ttgccgcatt gccttatttt
    61801 taccctaacc ggccatctct cggcttcaga cggcatatcg ggcggtttcc tgccggctta
    61861 cccgccgtga tactgccgag acagctcgtg tacggtatcg actaagattt tggcgtgttc
    61921 ggggtcggcg tgttggttga tgccgtgtcc gagattgaag acgtggccgc tgccgtgtcc
    61981 gtagtcggct aggatgcgcg cgacttcggt gcggatggat tcgggtgtgc caaagagagc
    62041 aaacgggtcg aagttgcctt gcagggcgac ttgcttgccg acgcggcggc gtgcttcgcc
    62101 gatgttgcac gtccagtcca agcccaatgc gtctgcgccg atttctgcca tgctttccag
    62161 ccacagcccg ccgcctttgg caaacacgat gacaggcacg cgcctgcctt cgctttcgcg
    62221 tttcagtccg gcaacaattt ggcggatgta tttgaggctg aattctttaa acgccgcatc
    62281 gctcaagacg ccgccccaag tgtcgaaaat ctgcacagcc tgcgctcccg cgtcgatttg
    62341 ggcgttgagg taggcggtaa cggcttgggc gttggtatcg aggattttgt gcagcaaatc
    62401 ggggcgcgag tacatcatgg ttttgatggt gcggaattct ttgctgctgc cgccttcgac
    62461 catgtaacag gcgagcgtga acggactgcc ggagaagccg ataagcggta cgcggccgtc
    62521 caatgcttta cggatggaag ttaccgcgtc gaaaacgtat tgcagttttt ccatatcggg
    62581 gacgtgcaat ttggcaatgt cgtcctcgtg ttgcagggcg cgtttgaatt tcgggccttc
    62641 gccttcggca aaatacagtc ccaagcccat tgcgtcgggg acggtcagga tgtcggaaaa
    62701 caaaatcgcc gcgtccaaat cgaaacgttc caaaggttgg atggtaactt cggtcgccaa
    62761 ttcggtgttt ttgcacaaat cgaggaagct gcccgctttc gcgcgtgtgg ctttgtattc
    62821 gggcagataa cgccccgcct gacgcatcat ccaaatcggc gtgtattcga cgggctgttt
    62881 aagcagggcg cggaggaagg tatcgttttt taaaagagtc atacgggttt tctgtcagtc
    62941 tgtttgtcaa gtgaaatggt tttcagacgg catttcaacg atgccgtctg aaggtttcaa
    63001 tgggtttcgg cggaaggtcg gttttcctcg gcaacgcttg ccaaaaccaa gttgcgctgc
    63061 gcctccgcct tctgcggttc tccggtttcg tcaaaaacct ttgccagaac caaacgcgcg
    63121 gaaatactcg gctttaatgc aatgctcgct tcaaggtagc cttttgcctt gccccaaagt
    63181 ttgcggccgt aggcgagccg accgagatac atcagcagaa gcgcgttatc gggctgttct
    63241 ttcagccaag catcggcaaa atcgatggct ttctgctgtt cgcgttcgcc caaaaagcgc
    63301 acgctttcga caaaggcttc caaaagctcg gggcggcggt tgtgcggata atgctgtttg
    63361 acccatttga ccgcatcggc atacagtccc aaacgttcgt acttttccgc aaccgatacg
    63421 ctcaattccc cgtttttgag gctgtcggga atccgcttca ggcaggtttt caaagcggcg
    63481 gcatcggcag catccgccag ctggcggcgg tatgcccaat tttgataccg ttccatttcc
    63541 gatttgccca acgcgcccgc cttggaaagt ttttcggttt ttgccagaac ctgcaacgcg
    63601 tcgcccctgt cgaaagcgta acgaagttgc agacgcacga ggcgcgtaag gttggcattc
    63661 atcttcgccg ccgcatgaag attggcttcc gccgcttcgt aatcgcgccg gttcaacgcc
    63721 gattccgcca acaaaagata acgggaaagc tgctgctttt ccggcagttt ggcgatttcc
    63781 gcaagataac ggtcgcgcag ctcgatgttt tccatctgcc cggcggcatg tgcgcccaac
    63841 atcaatgcca aagtccggtt gtctccggcc tctttgttga ccaacacgcg tgaggcttct
    63901 agttccgcct tttcaaaacg cccttcaaaa tacgccaaac ccgccttgtt caaggcaagc
    63961 gcggccttgc ggcctttacg cgccgaaccg aaacgctgca tcttttcggg gatattgaat
    64021 acgccgataa tgaatttaaa caggaaatac cacaccacga cggcaatcag cgaacctaac
    64081 acaaaggcgt gcaggttgat tctgagcatg gtctgtccga gtacgatata cacgtcgccg
    64141 gtgtaaatgc ccgaagccaa tgccagcccg acggcggcgg caaacaggac gacaatccag
    64201 actaccgttt tcatgcgcgt tctcctttag cggacggcga acgccctgcc ttttccggta
    64261 cgggctgttt ttttgcaggc tgttccggtt tgcgttccga aggcagggac ggtgcttcta
    64321 tggtcttcac atccgatgcg gcagccggtt cggaagctgt gttttcgttt gccggttcgg
    64381 aagccgcctg ttctgcttct tgattttccg ccgccgtcat gcgcgtaccg tcgcgatagg
    64441 cgcggacggc atttaggctg tttttcaaac cgtcatccgc cgtcatgcgc acatccaacg
    64501 ccttcaattc cgccagttct ttcagccacg actgcgtggc gggagacttg gcatcgaaat
    64561 actgtctgac ggcggcttcg gcattgttca aatcgccctg atagacttcg ctgttgcgct
    64621 gcattaatgc agtccgcgca tccaaaaggc ggaggcgcag gttttcacgc acaaaatatg
    64681 cctgttcggg agaaatcagc atggcatcgt tgttttccaa acggcggatt tcgaccagcc
    64741 ccttcaatgt gccgagggat ttttcccata cgttctgcca ccatgaagca gaagcggctt
    64801 cgttcttcac ctgtacgccc ggcttcaata cgccgtccaa aatcagcggc agtccggata
    64861 cggcggtttc cagcctgtcg aggcgcaatg ccgtgccgga aatatcgaca tagggacggt
    64921 ttttcagttc cgccaagtcg ctgctgactg cctgtttgat cggcagaagc tctgcctgat
    64981 cgaaacggga caggcggctg tcgatatgct ccaatacgcc gactgccgtt tggatattgc
    65041 cggtcaacac cagctgttgc gccgccagat tgagtatggt ctcggtttcg tccaccagcc
    65101 aatcggcgcg tcctttggtc aactcgcgat aggatttttg catttccaag atttgttcgc
    65161 cgtttgcttt gacgtttccg tccaaacggt cgagctctga ttgtatggcg gcttgccggt
    65221 tgaggttgtc tttcaacagg gcggcgtttt ccgactcgcc caaggcggct ttgtcgattt
    65281 tttggttgaa tgccagctct tggtttttca agacattctg tccttggaca aacaaaaaac
    65341 cacttgtacc caaacccaat gccgccaata ccagggcgca gactgccaaa gcgttgctgc
    65401 cggactgttt gacgataaaa ggagcggcag gaatgtgtac ttctgtttcg ttttctttgc
    65461 gtggggaaga ggtttcagac ggcatttctt ttgatgcctg tatctcgcgt acgggttcgg
    65521 atgatttgtt ttcaggttcg cccaccgttt tgctccttat taatttgagg ttccgggaaa
    65581 gaccatgccg tctgaaacgg aaatggaaga atgggaaagc gcggcttcca gcgtagggac
    65641 ggtttcgacc gaacacacgc cttcgcgctt caatgcctcc gcaatgcgcg gatgatgggt
    65701 aaagtatagc aaggatttga agaatcggga aaattgcggc ggaagctgtc cgaacagcaa
    65761 gcgcacgagt tctgtggacg taatataggc ggcggcaata ttttcggttt ggaaattttg
    65821 aaagttcaaa ggtttatgcc gtctgaaata gacttctgcc acctccgtcc gaaaaccttt
    65881 ctcctgcaag gcattcatca gaaaatcccg cccgccgtgt ccgcgcacaa acaatacgcg
    65941 cgcaccttcg ggcagactgt tccaaaccgt caggcgcaaa accgcctcgc tgtcgttgcc
    66001 gtcttcaggc gcgatgaccg ttctgcccaa acagcgttcc aatacgcggc ggctgccctg
    66061 ccctacggaa atgtgcatct ttatgccgtc tgaaaggtta aggtacggga cggcggtttc
    66121 aacggcggtc ggactgaccc aaaacacggc atccgcacgg gcatattgtt cggacaaaag
    66181 ttccagtccg gcagcatctg cttcgatttc aatcgggctc aacacttccg cctgccatcc
    66241 ggcacgacgg caggtttcca catcgtcctt cgccctgccc gacggtcgga caatcagcat
    66301 gatgggtttc actgtttcca tcgccttatc cgccccgatt accgggaaaa atcagcatcg
    66361 ttgaaattcg cctgcgcggt ttgcggcgta cagccaaccc gtgcgacttc cttgccccta
    66421 cgctccacaa caacaccgct gctttccgta tgagtcacgg aatcgaagcc cgtccatacg
    66481 gtgtacataa attcgccgtt ttggaatttc atcgttgccc ggcgtccgct acccatgcct
    66541 tgccacctgt cggaacgccc caacaggtca gctttgctgt tgcgtatggc aatttctttt
    66601 tttgccgcac tgccgaacga atattcgtaa agattgcggt tgattttttg aacttctata
    66661 tatttgttgt tgtccgtttt acaggaaaac accgtatccg cccacaccgt ttgggcggca
    66721 gccgacagaa gcaatacagc agaaaggaac atttttttca tcgtatttcc ttaatggttg
    66781 aaactccgcc atttggacat ccgtccttcg tggcagcagc atcagatgtt atttgagagg
    66841 ggtgtaaccc cttccaaatt agggcaacac atagggcaac gctttatgtg tcgtcctgtg
    66901 tgttgaaaca tcattgcccg tatcctgaaa tagaaaagaa aatgcccgtt ttacggtctt
    66961 ctttccgaca aaatgccgtc tgaaacggat ttcagacggc atggtcgaaa attattgcgc
    67021 ggcaagcggc ttgagcagca aggtttcgcc gctttgcaca actttttcaa gcaggtcgat
    67081 attgggatgc ttgccgggat gggcgcgcgc gtcggcaacg tgttcggcaa accatatcaa
    67141 accctgttct gccgcttctt tgtccaattt tccgtcaaaa cgttctgcca aagcgttgta
    67201 caacttgagc gaaccgagct taccctgaac ggcaggaatg tggtgaaccg ttttgccatc
    67261 tgcatcctga acgtccaaac cgcccagatg gtcgattgaa ggaaatgtgg cgagatagtc
    67321 ttgaaaattc atatcaactc cgaaaatcag tcagttagcg cgtgccgaaa atcttgtcgc
    67381 ccgcatcgcc caagccgggg atgatgtagc cgttctcgtt caagtggctg tcgagcgcgg
    67441 cggtgtaaat cgtaacgtcg ggatgcgcgt cgttgaccgc cttcacaccc tcgggcgcgg
    67501 caaccagcac cagtgccttg atgtttttgc agcccttggc tttcaaaagg tcgatggtgg
    67561 caaccatcga accgcccgtc gccagcatcg ggtcgataat cagggcagga cgttcgtcca
    67621 tgctgtccac gaatttctcg aagtaggaaa cgggtttcag cgtttcctcg tcgcgctgca
    67681 gtccgactac gctgattttg gcagtcggaa tcaggtcgag tacgccgtca agcataccca
    67741 aacctgcacg caggatggga acgacggtca atgttttgcc cttgatgcgg tcgccttcaa
    67801 tctgaccgca ccatccgtcg ataaggtatt tttcgatttc aaaatcacgg cttgcctcgt
    67861 atgccatcag gcgcgccagc tcgatggcaa gcgtccggaa tttgtaggtg ctgcaatccg
    67921 cctccctcat cagggttaat ttgtggcgga cgagcggatg gttgataaca ttaacgttca
    67981 tcgcggtgtt ccagtgtgat tttgagacgc gccattatac ttttttttga tttgccggaa
    68041 aagaaaatgc cgtctgaaac acgttcagac ggcattgccg aatccatcag gctttcagca
    68101 gctcttgcag ctcgcctgcc tcatacattt ccatcaggat gtccgaaccg ccgacaaact
    68161 cgccgttcac ataaagttgg gggatggtcg gccagtcgct gtattcctta atgccttggc
    68221 gtacttcggg attttccaat acgttgacgg taacgtaatc ggtgcagcct gccgcgttca
    68281 ggatttgcac ggcgcgggaa gagaaaccgc attgcggaaa ctgcttcgta cctttcataa
    68341 acaatacgac gcggtgtgtc gttactactt ctttaatttg gtcgtggatg gaagccatcg
    68401 cttattcctt ttgataaagt tgacaaaatt tgtcaggtag gacgcattat acgcaaaagc
    68461 ggaaacttcc cacaatattt gccgtatatc gcgcagtatc aatgttgtga acggaaatat
    68521 tcccactcct cgacaatcct gctgtccggc aaatggcaca gggcatatcc gccgccgctt
    68581 tcgtcttttt ttacttcccg tctgccgcct ttggcaatgc agaatttgga agcggaattt
    68641 gccatgcctg tccgaaacgt gttggcaggc agccggcagc agcgcggaca tgcatacgga
    68701 tgaattgcct tcatggtttt ccttatcgtc ggtacagggt cagacggtcc gcatatcggg
    68761 tactttttta aacagccggt aaaagttttc cgtcgtatat gccgcaacct gttccaatgt
    68821 ttggttccgc aatttggcga tatgttcggc ggtatggcgc acaaaagccg gttcgttctg
    68881 cctgccgcgt ttgggaacgg gggcgaggaa cggcgcatcg gtttccacca aaatgcggtc
    68941 gtccggaacg tattttgccg cctcctgaac caagggtgcg tttttaaagg taacgattcc
    69001 cgagaaagaa atataaagcc ccaaatccat tgctgcacgg gcaaaaccga tgtcttcgga
    69061 aaaacagtgg ataacgcccg aattaacccg gcattctttc aggatagaca aggtgtccgc
    69121 cgccgcatca cgcgtatgga cgataacggg cagtccggtt tgattggctg cttcgatgtg
    69181 gtctgcaaag cgtttgtgtt gccaggacaa atcgcctttg caccagtaat aatccaaacc
    69241 cgtctcgcca atgccgacca ctttcggcga ggcggctgcg gcaaccattt ccgcaataga
    69301 aaattcttcg gcttccttgc tgtcgggatg tacgcctatg gtgcaataga tgtgttcgtg
    69361 cgcttcggcg atggcaaaga cttcggagaa gctttcccta ctgacgctga tggcgagcgc
    69421 ctgccccacg ccgtttgctt ccatgttgga caaaacttcg ggcaggcgtt ctttcaaacc
    69481 ttcaaaattg aggtggcagt gcgaatcgat gatatgcatg gcgttacatg gtaaacgtag
    69541 gtctgctgcc gccgattgtg ttgcccagtt cgacttcgat ctggtctttg actttcaggc
    69601 agttttcgtg ttttgtgaat gccagcccca cccctttggg tttggaggaa gtacgcgcag
    69661 gattgatcca ggcgactttg gtcggcagga acagtttggg gaagttgagg atttctacgg
    69721 caagcagaat atcgtccccg atggaaaata cgtcgttggt ctgcacaaac agaccgccgt
    69781 gttccaaaaa cggcatgtag gagctgtaca gcagattcat gtctttcagc tgcaacgaca
    69841 tcatttttgc cggaatattt tgtccgtctg acataattca cccgttctct ttcttcaatt
    69901 ccaaatagtt gatgagcaga tgctcgatct gcattttgac atttaaagta tgaaacccgt
    69961 agggggcaag ctgtttgagc atatcctccg ccgcaaatac attgcgcagg cggaaaccgg
    70021 ataccgtctg aagcagcctg tcttcataag cgggataata gacgggtttc atgtgttgca
    70081 ggcacaatcc caaatcgacc agccatttct gcatccaccc gacaaatacg gcgagcggaa
    70141 gtttttcctt atcgaaaagc gcggcgtaat ccaaaatctt caacaacctt ggttctgcca
    70201 aaatatccaa cagtttaatc cgcaacgcac ggacaccgtc cgcctcatca aacagcggcg
    70261 ctccggaatg gaaagccaga cgttcctcag gttccgccac acccctttca cgcagatatg
    70321 ccaatgcctc ttcatgggaa ggcgcgggca aaaccatctt ccggcagcgg cttttaatgg
    70381 tcggtaaaac cttgtccgcc gcgtggctga ccagcaaaaa gaccacttgt ggcggcggct
    70441 cttccaacac tttcaacaaa ctgttggcgg cttggacatt catactttcc gcaggatgaa
    70501 tcagaatcac gcgcaaaccg ccccgtaccg aagtcaggta cacattatcg atgatttccc
    70561 tgacggcatc gattttgatc tgcaacagtt tgcgtccgtt ttcgggttcg tccgacaagg
    70621 gggtgatttc gtaaaaatcg ggatggcttc cctgtccaaa cagatggcag gacatacatt
    70681 cgccacaggg tttgcagccc ggtgcagggg tttcgcacaa cagtgccttc gccgcaaagc
    70741 gggcaaatgt agttttcccc gtatcttttt tgccggcaaa cagccatgca ttgggacgac
    70801 gctcccaatg ttccgcaatc tgccgccatt gctcattatg ccacggataa atcatttcaa
    70861 accatccgtc cggaaatcca taccgccatc agagcagttc caaatcgggg ctggaacttt
    70921 gcgcgcggcg gctgcgcaac tgtaccgcca aacgcaaatc gtgtgcggaa tcggcatttt
    70981 tcaatgcttc ttgcagggaa atatcgcctt tttcatacaa ttggtaaagg tgttgatcga
    71041 aggtctgcat acccagggta gtggattttt tcatcacttc tttgatttca tggatgttgc
    71101 cgttgtgaat caactccgaa atcaggggcg aattgagcag cacctcgact gccgccaccc
    71161 tgcccttgcc gccgtctcgc ggaacgaggc gttgcgaaat aaacgcctga aggttgagcg
    71221 acaaatccgt cagcaattgt tcgcgccgct cctcggggaa aaagttgatg atgcggtcga
    71281 gtgcctgatt ggtgctgttg gcgtgcagcg tcgccataca caaatgcccc gtttcggcaa
    71341 aggcaatggc gtagtccatt gtttcgcggt cgcggatttc gccgataagg atgacatccg
    71401 gcgcctgacg cagcgtgttt ttcaacgccg ccatccagtt ttccgtatcc acgccgacct
    71461 cgcgctgggt gatgatgcag tttttgtgtt cgtggacaaa ctcgatcggg tcttcgatgg
    71521 tgatgatgtg tccgaacgaa ttttcattgc ggtaatcgat aagcgaggca agcgaagtcg
    71581 atttgcccga gccggtgccg ccgacaaaaa taaccagccc gcgttttttc agcgcgacat
    71641 ccttcaagac cggcggcagg ttcaggcttt caaacttggg aatcttgctg gtaatcgcac
    71701 ggaataccaa cgccgtcgcg ccgcgctgta tcatcgcatt gacgcggaag cggctggtgt
    71761 ccggcaggct gatggcgaag ttgcactcgt tggtcgatga aaattcttcc gcctgcttcg
    71821 cactcataat cgaaaaggcg atttccatac atttttccgc cgtcagcggt tcgtccgtga
    71881 tgcgggtgat tttgccgtcc agcttcatcg cgggcgggaa atgggtcgtc acaaacaggt
    71941 cggaaccttt gtttttgttc atatggcgca gccatgcgaa cagttcttcc tttgcggtca
    72001 tattttcatc ggtaaacatc ctcgtcccct aatcttcaaa caatctgtac cttcccattc
    72061 taccaaaaac ccgccgtgcc gtctgaaacc gtttcagacg gcacggacaa accacctcca
    72121 ctcaatcctg ccgtgttagc gtgtataatt cggcattcta tccgaaattc acagcaggtc
    72181 agcaatatgt ctttgaaaac agtagccgtt atcggcgcaa tggaacagga aatcaagctt
    72241 ttgcgcgaga tgatggaaaa tatcaaagcc gtctctttcg gcagattttc cgcctatgaa
    72301 ggcgaattgg cgggaaaacg catggtgctt gcattgagcg gcatcggcaa ggtcaacgcg
    72361 gcggttgcaa cggcttggat tatccgtgaa ttcgcagcgg actgcgtcat caacaccggc
    72421 agcgcgggcg gtttgggcaa gggtttgaaa gtcggcgacg tagtcatcgg cacggaaacc
    72481 gcacaccacg atgtcgacgt aaccgcattc ggctatgttt ggggacaagt gccgcaactg
    72541 cccgccgtat ttgtttcaga cggcctcttg gtcggcaaag caaaacaggc ggcggaagtg
    72601 tttgaaggcg cggcggtaga acaaggcctg attgtcagcg gcgaccgctt cgtccacagc
    72661 agcgaaggcg tggcggaaat ccgcaagcac ttccccaaag tcaaagcagt ggaaatggaa
    72721 gcggcggcga ttgcccaaac ctgtcatcag ttggaaacgc ctttcgtcat catccgcgcg
    72781 gtttccgatt cggcggacga aaaggcagac atcagctttg acgaattttt gaaaacggcg
    72841 gcggcaagtt ctgcaaaaat ggtggcagaa atcgtcaaat ctttataaaa cctttatcaa
    72901 atcatgccaa aacagcggtt ttccgctaga atgtcgggct gtttatcccg ttgcgcccga
    72961 cattcggacg cagcgccgtt tgttcagacg gcatttccaa aacccactgt ccgacattca
    73021 aaataactct aatgaaaaat atccgaaatt tctccatcat tgcccacatc gaccacggca
    73081 aatcgacgct tgccgaccgt tttatccaat actgcggcgg cttggatttg cgcgaaatga
    73141 gtacgcaggt actcgattct atggacatcg aaaaagagcg cggcatcacc atcaaagcgc
    73201 aaaccgccgc gctcaactac aaagcgcgcg acgggcaggt gtatcagctc aacctgattg
    73261 acacgccggg acacgtcgac ttttcttacg aagtttcccg ttcgctgtcg gcctgcgaag
    73321 gcgcgctttt ggtggttgac gcatctcaag gcgtggaagc gcaaaccgtg gcgaactgct
    73381 acaccgcgat tgatttgggc gttgaagtcg tgcccgtttt gaacaaaatc gacctgcccg
    73441 ccgccgaccc cgaacgcgtg gaacaggaaa tcgaagatat catcggcatc gatgccgtcg
    73501 gcgcggtgca atgttccgcc aaaagcggca tcggcgtgga agacgttttg gaagaaatcg
    73561 ttgccaaaat ccccgcaccg gccggcgatg aaaatgcgcc gctgcaagcg gtgattgtcg
    73621 attcgtggtt tgacaactat gtcggcgtgg ttatgctgat tcgtgtgaaa aacggcacca
    73681 tcaagctgaa agacaaagtg cgctttatga gcaccaaggc ggaaacgcag gttgagcagc
    73741 tgggcgtatt cacgccgaaa tcagttcaaa aacaagaact caaagccggc gaagtgggct
    73801 ttttgattac cggcgtaaaa gaattgggac aggcaaaagt cggcgataca gttactttga
    73861 ttgccaaccc cgcctccgag ccgctgcccg gcttccaaga agtgcaaagc caggtattcg
    73921 ccggacttta tccggtggaa agccacgact acgaagcctt gcgcgacgct ttggaaaaat
    73981 tgcagcttaa cgatgcttcg ttgaaattcg agcctgaagt ttcccaagca ttgggtttcg
    74041 gcttccgttg cggcttcttg ggcctgttgc atttggaaat cgtgcaggaa cgtttggaac
    74101 gcgagttcga catggacttg attaccaccg cgccgacggt ggtttatgaa gtcgtgttga
    74161 aaaacggcga gaaaatcgaa gtcgaaaatc cgtccaaact gcccgacatc ggcagcatcg
    74221 aaaccattct cgagccgatt atcactgcaa ccatcctcgt gccgcaggaa tatgtcggca
    74281 acgtcatgac tttgtgcaac caaaagcgcg gcgtgcaagt caatatgcaa tatatgggcc
    74341 gccaagtgat gctgacttac gacttgccga tgaacgaagt ggtgatggac tttttcgaca
    74401 aactcaaatc tacttcgcgc ggctatgctt cgttggacta ccatttcaaa gagttccaac
    74461 cgtctgattt gattaagctg gatatcatgg tcaacggtga aaaagtcgat gcgctgagcc
    74521 tgattgtcca ccgtcaaagc gcggttcacc ggggccgcga actggcatcg aaaatgcgcg
    74581 agctgattcc gcgccaaatg tttgatattg ccgtccaagc cgccatcggc agtcagatta
    74641 tcgcccgtga gaacgtcaaa gccctgcgta aaaacgtttt ggcaaaatgt tacggcggcg
    74701 atattacgcg taagaaaaaa ctgcttgaaa aacaaaaagc aggcaaacgc cgtatgaaac
    74761 aagtgggcaa tgtggaaatc ccgcaaagcg cgttcttggc aattttgcag gtgagcgaca
    74821 aataatatcc ggcacacggg gcagtgcatc atgcccctta aaccgccctg attgtaagag
    74881 agcacgtccg ccaacaaact ttgtgattta ccgcctctta aaaacggttg cacggacggg
    74941 cgggttttta accgttaagg aacaacaatg aacattatgc taatgtcggg cgcggctgcc
    75001 gcgctgcttg ccggcatcat cctttatttc aaaagcgaca aaaagcggca ggaaaacggt
    75061 gaatggagtt ccggtctgga atacgcctat atcctgacgg cggtcggcgt gtttgccgct
    75121 ttgtccctgt ttatgagctt taccgccgtt ttcctgattt tcgttgtatt gtgcggtacg
    75181 gcttgggggg tatacaaata ccgcctgaag actcatcccg aaatctcgga aagcagccac
    75241 ttcggcgatt atttcggcag tttcttccct accgttttgg tattgttcct catccggtcg
    75301 tttattgccg aaccgttcca aatcccgtcc agctcgatgc gcccgggcct gattaagggc
    75361 gatttcattt tggtcggcaa attttcctac ggcctgcgcg tacccgtttt aaacaatgta
    75421 ttcatcccca cgggcaaaat cgaacggggc gatgtggtcg tttttaatta tcctttgcaa
    75481 ccggagatga cctacatcaa gcgtattgtc ggcattccgg gcgatgtggt cgaatatcgg
    75541 aataaagttt tgacggtaaa cggaaaacct gcttccgata ttcctgacgg cacataccgt
    75601 tatcccgacg acaccgaccc atccgaaatc cacaatacgg atatgttcca cagcggtttg
    75661 gacggcaaat ccttcaatat tctgaaaaaa gaaggacagc ctgccgtttc cctgcccgta
    75721 ttgggcaaat atacctccga tattatgtct gaaaacggat attccataga gcaaagcggt
    75781 ttggaacact gccaatatgc cgacgacggc agcggtttcg tgtgcaaagt tcccgaagga
    75841 cgctatttcg ctatgggcga caaccgcgac aacagtgccg attcgcgcta ctggggattt
    75901 gtggatgaca agctggttgt cggcaaggca atgttcattt tgatgaactt cggcgatttc
    75961 ggcagggccg gtacggcaat ccgttaggcc aggaagccaa gaatgccgtc tgaaaatagt
    76021 ttcagacggc atttgctatt cctcactttt gcaggcggat ctaacgttgt cggattttcc
    76081 tacctatagt gaattaaatt taaatcagga caaggcggcg agccgcagac agtacaaata
    76141 gtacggcaag gcgagccaac gctgtaccgg tttaaattta attcactata tcagtccggt
    76201 aacgcaggtt ttgcacggaa aactgccaat actgccttga tggaatcaat attaagactg
    76261 ctgtttcagc gaaccgaggt aaattgccga aagcgtcagc gccacccctg cccactgcac
    76321 gccttcaatc ggtttgccca acccgaaata atcgatgaac agggcggcaa ccggttcgga
    76381 caaaagcagc agccccgtca gcgacagcga aagcagcgga atcgcatagg caaccatcgc
    76441 ccacgcgaag cactgcatca ccacgccgta cacaagcacc aaacccgcat ctttccaagt
    76501 cgtcggataa agcacgccgc catccatcag caatgccgga acaaccagcg ataccgcgcc
    76561 gcccaaactc aaaatcatca ttgaagggaa aagcgccacc ggctcgattt catgggtttt
    76621 gcggacgaac accatcgaca gggcgagcat cagtcccgat accaaaccgc tggcgaatcc
    76681 ccaaaccgca ttaccgttgt agccgaattc cgcaccggca atcatcgcca cgccggcaac
    76741 tgccgatatt aagcctgcct ttttcagccc gctcaaacgc tcgccgaaaa agaaaacacc
    76801 gattgccgac aagaaaaaga tttgcaggct gttgagcagg gtggaaatac ccggcccgac
    76861 cgcgtgtatg ctttcgtgcc acaacgccaa atcgaaagcg agaaacacgc ccgccgtcag
    76921 ggcatatcgg acggttttcc tgttttttgg gaatttttgc ccgaaaaacc gtgctaaaaa
    76981 ccaaaatacg aacaccgaaa tcagcaaccg ccaaaatgcg attgcatacg aaccgacggg
    77041 gacggatctg acaatcagac tgcccagacc gaacaccacg caacccagca ccagcaatgg
    77101 tgcggcatat tttttgccgt taccgaccac aatatcccct cccttcccca tacggctgcc
    77161 gcgccgacgg caagaatgcc gccattccga aaatatgccg tctgaagccc aaagccccgg
    77221 ctgcacccgg acaaacggct gattttacgg gcaggcaggc tattttccgt tggggcaaaa
    77281 aaccgtcctg ccggataaaa cccgaatata gtggattaac aaaaaccagt acagcgttgc
    77341 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
    77401 cgtactatag tggattaaca aaaaccagta cagcgttgcc tcgccttgcc gtactatctg
    77461 tactgtctgc ggcttcattg ccttgtcctg atttttgtta atccactata tctgtactgt
    77521 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataaaaaat atttccctca
    77581 agcgatgtgg cggcaccaac acctgattaa aacacaaccg gcgttttcgg catatacgca
    77641 aaaacgcatt ttccggcacg gcgttacacc gataccgaaa aatgcgtctg aatgtttcga
    77701 caatccgatt aaagcattat gccaaatctg caaaaagtgg cgtagagaga tagcgttcgc
    77761 cataagaagg cagcagcacg actatcagct tgccttcgtt ttcaggctgt ttggcaagct
    77821 gcaacgcact ccaaaccgcc gcaccggaag aaatacccac caaaatgcct tctttttccg
    77881 ctattgcgcg ggcggtttca aaagccgctt cgttcgacac tttggtaatg ctgtcgtaaa
    77941 ttttggtatt caaaacggtc ggaataaagc ctgcgccgat gccttgaatc gggtgcgggc
    78001 ctttttcgcc gccgctcaat acgggtgaag cttcaggctc gacggcaacc actttaactt
    78061 cgggtttgta ttttttcaac acttcgccca cgccggtaat cgtaccgccc gtgccgacgc
    78121 cggcaacgaa gacatcgact ttgccgtccg tatcccgcca aatttcctcg gcggttgttt
    78181 tgcggtggac ttcggggttt gcctcattgt cgaactggcg cggcataaaa taagtgtcgg
    78241 gatgcgcgtc caccaaggat ttcgctttgg caatcgcgcc cgccatacct tcggcggcag
    78301 gggtcagaat cagctccgca ccaaacgcgc gcaacagcat tttgcgctcc ttactcatgc
    78361 tttccggcat ggtaatcgcc agcttgtagc cgcgtgcggc acataccatt gccaaaccga
    78421 cacccgtatt gccgctggtt gcttcgacaa tgacggtgtt tttgttgatt ttgcccgctt
    78481 tttcggcacc ctcgatcatt gcttcggcaa tgcggtcttt gacgctgctg cctggattga
    78541 aaaattccag tttcacggca acctccgcct tcaaaccttc ggtcagacgg ttcagtttga
    78601 ccaaaggcgt gttgccgatt agttcggtga tgctgtttgc aattttcatc ttctgctcct
    78661 gaataataag gtttcgacag taaggcgcaa tccccatagg catcctgtcc gattgcaggg
    78721 gatttgcgcc gaaacataaa caaatttcgg atatggcttt atcctaaacc tatgccgcct
    78781 aaaaaaccaa tatccaaaat ttctttcttt ataacttttt gttccgatac ggccgcaccg
    78841 tattatcttc caatttctga aagcagcagc accagtaccg agacctgcat caacgctgcc
    78901 gtcaacccgc cgagtcgcag ctgcgagccg ccgaacgttg acggcggctg ccggtctata
    78961 ccgcagcggt ggacataggc aaccgccatc agcacaaact gcaacacgaa ccaataaacc
    79021 ggcttcagcg cataaagttt gagcagtgcc gcaaacacaa acacttgaac cgttcccgaa
    79081 aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga tacattactg
    79141 acggcaaaca gcccgagcag aggatgttcg gggtctgcct gccatccgtt tccatccgtt
    79201 ttccggttcc aatgcccgat gaaaaaaaat atgctgccca aagtcaggta aaaatggggg
    79261 atgaacaagg gcgcggcgcg ggtcattccc catatgccgg gcatcagctt tgcccccaaa
    79321 accgatatgc ccagccacag cataatactc gcccaaaacc attgcaacat tcctgcgcgg
    79381 aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc caaatgcagc
    79441 aaaatcatga atacgccaac tcgccttcaa acaccgtttc cgcagggccg gtcatcatca
    79501 catcgccgcc gcaggcccat tcgatatata aagttccgcc cggcaaaatc acctctaccg
    79561 ttttcccttc atccaacaac cccagacgga tacccgccac cacagccgca cacgcgcccg
    79621 taccgcacgc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacga atcgcggttc
    79681 ggctgataat ctgcataaag ccgacgttga cgcgttcggg aaactgcctg tgcggttcga
    79741 taagcgaacc ggtttcgcgc accggcgcgc attctacgtc atcgaccaca atcaccgcat
    79801 gggggttgcc catattgacg cagctgacag gctgaatgcc ggattcgaga tgcaccccgt
    79861 aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca gacggcataa
    79921 acctcggttt gcccatattg accgtaacca tgccgttatc ggacaatttc ggaaaaataa
    79981 cgccatttgc cgtttcaaca caaatttctt tcttatcggt caaacccttg tctgcaacaa
    80041 aacgggcaaa acaacgcgct ccgttgccgc attgctcgac ctcgctaccg tcggcattga
    80101 aaatacggta acggaaatcc acgccttcgg tttccgaacg cccgaccacc aaaagctggt
    80161 cgaagcccac gccccggaag cggtccgccc atgccgcaat cggcgcgtcc tcgggggtaa
    80221 aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc attttggtaa
    80281 atttcaaagt gttcatattg ttccgaaatc ccgtatcgtc tgttgcttcc gaatccgaaa
    80341 tcccccgcac cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa aatcagccat
    80401 tatactgttt gttacccctt acaagaaaca ccatgttccg catattactg ttgcttttcc
    80461 cttgtgcaat ccttgccgcc tgcgcccgaa acataccggc aagccccgcc gtttcaaatt
    80521 gcgcgcatct tcaaaaccgc accatcctgt attttgacgc acacggcaaa cccgccgacg
    80581 gcgcagtaaa gggcggattt tacaaaatta tcgaaataca gggtgcaaac tgcatcctta
    80641 ttcaagactt taacagcacg ggcaatgccc tcggcggatc ctatgtgccg gactgccgca
    80701 aatcatccct tttcccgaaa aatatttgac ttcaaactgc ctatgaacca cgacatcact
    80761 ttcctcaccc tgttcctact cggtttcttc ggcggaacgc actgcatcgg tatgtgcggc
    80821 ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgatt ttggctgatc
    80881 ctgctgctta acacaggacg ggtaagcagc tatacggcaa tcggcctgat actcggatta
    80941 atcggacagg tcggcgtttc actcgaccaa acccgcgtcc tgcagaatat tttatacacg
    81001 gccgccaacc tcctgctgct ctttttgggc ttatacttga gcggtatttc ttccttggcg
    81061 gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc tgaacccgat actcaaccgg
    81121 ctgttaccca taaaatccat acccgcctgc cttgcggtcg gaatattatg gggctggctg
    81181 ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg gaagcggtag tgcggcaacg
    81241 ggcgggttat atatgcttgc ctttgcactg ggtacgctgc ccaatctttt ggcaatcggc
    81301 attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct gtgtacggga
    81361 ttatccgtat cattatgggc attatggaaa cttgccgtcc tgtggctgta agccccattc
    81421 attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggcact ttcaccctaa
    81481 gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt tgatggacag
    81541 acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct gaccgacttg
    81601 caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt gtaccaaacc
    81661 gtctttgccc ggcatcacgc tgacaatcgc gccgacgttg ttgtcgagga ttttcaccac
    81721 agtgccttca tacactttgc ccacttccac ttcggcagta atctgctcga tgcgtttttt
    81781 cgccgcatcg ccggcttctt gagtggtcgc ggcaatggta atcgtaccgt cttcggcaat
    81841 attgatttcc gtaccggttt cagcggtaat cgaacggatg gtttcgccac ctttaccgat
    81901 aacttcgcgg attttgtctt ggttgatttt catcgtaaac aaacgcggcg cgtgtgcgga
    81961 caactcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca gacgcgcttc
    82021 tttggcctgt gccaaagcga tttgcataat ttctttggta atgccttgga ttttgatgtc
    82081 catttgcagc gcggtaacac cttcggtcgt accggccact ttaaagtcca tatcgcccaa
    82141 gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat ttgttgcctt ccagaatcag
    82201 acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca gcaggctcaa
    82261 acagccgccg cagacggaag ccatagagga agagccgtta gattcggtaa tttcggagac
    82321 cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca atgcacgttt
    82381 agccaaacga ccgtgaccga tttcacggcg tttcggcgcg cccatgcggc ccacttcgcc
    82441 ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtgtatt cgccggacag
    82501 cgcgtcgatg atttgctcgt cgcgcgaagt acccaaagtc gctacggcaa gggcttgggt
    82561 ttcgccacgg gtaaacaatg cggaaccgtg cgtgcgcggc aatacgccgg tttggatgtt
    82621 cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttggccgt ccaaaatttg
    82681 gctgcggacg acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt tggctgccag
    82741 cgtgtcggtt tcttcggtaa tcaaggcttc ttttaccgca ctccaagctt cgtccaattt
    82801 ggcagaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt cgccggcaat
    82861 cccgcggact ttggcaacca gttcctcatt ggtttcaggt gctttccaat cccaaacttc
    82921 cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca tttgatcgtg
    82981 gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt cggattccac
    83041 catcaacacg gctttggaag taccggcgac caccaagtcc aattgcgatt tcgccaattc
    83101 ggctttagtc gggttcaaaa cgtacacgcc gtttacataa ccgacgcgtg ccgcgccgat
    83161 cgggccggca aacggtacgc cgctcaacac cagcgcggca gacgcgccca acattgcagg
    83221 aatatcagaa tcgatttcag gatcgacgga cacgaccatc gctacgattt ggatgtcgtg
    83281 gtagaaacct tcagggaaca gcggacgaat cggacggtcg atcagacggc tggtcaggat
    83341 ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg ccgggaattt tgcctgcggc
    83401 gtaagtgcgc tcgaggtaat cgacagtcag agggaagaag tcttggcctt ctttcacttc
    83461 tttgttggtg gtaacggcaa ccaaaaccac ggtgtcgccc atagagactt taacggcagc
    83521 ggcggcttgg cgggcaattt cgccggtttc caaagtaacg gtgtggttgc cgtattggaa
    83581 agttttaacg tgtttgtcga acattattgt tcctttcaaa ataccgcact gctaaaacac
    83641 taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt gcggcaggtt
    83701 gtaaacaagc tttcagacga catttcggac gctgaaagca attccagatt ataaaggcaa
    83761 ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaccata tttctttcag
    83821 acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc ccgatacttc
    83881 tcgacagttt tctgaatcac atcggcaggc actttcggcg caggggcttt tttgttccaa
    83941 ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg attggtgccg
    84001 actttgtatt ggtcggtagg ccaaaaacgg ctcgaatcgg gagtcaatac ctcatccatc
    84061 agcgtcagcg taccgtttgt atccaaaccg aattcaaatt tggtatcgca aataataata
    84121 ccgcgcgatt tggcatattc cgccgcttcg gtgtaaagcc gaaccgcctt ggcgcgcact
    84181 tcttccgcca attctttgcc gataatgcgt ccgcattctt caaagctgat gttttcatcg
    84241 tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag ttgttgcgct
    84301 tcctgcatac cttcaggcag ttgaataccg caaaccgagc cggttttttg ataatctttc
    84361 caaccgctgc ctgccagata accacgcaca atcgcctcta ctttcaccgg agtgagcttt
    84421 ttagccacga cggcgcgttt ctctaaagct ttggcttcgt tttcaggcaa aacatcgtaa
    84481 accgtttgac cggtaaagtg gttgggcata atatgcacca gttttttaaa ccaaaaattg
    84541 gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat cacatcgaat
    84601 gcagacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc atataaatcg
    84661 cgcacttttc caaaatagat ctttaccaaa ccaatctcac tcatttcgcc ccccctgaaa
    84721 atatcttgaa aataccgacc cgacacccga caggtttgaa tcacaaacca atattctagc
    84781 cgaagtcggc gcaaaacaat acccatggca caaaaaacca acccgtcaac cgtcggcaaa
    84841 gttttggcac tataataccg acagcaagtc ctacaataca cttttaccaa aggaaatacc
    84901 tcatgagaat cctattgaca ggctcgaaaa gccaactggc acgctgcctg cgcgaccgtc
    84961 ttccggaaga ctgggaaacc attgcgacgg attccgcatc cctagacatt accgatgccg
    85021 atgccgtctg caacatggtc aaaagtttcc aacccgacgc cattgtcaac acggctgcct
    85081 atactgccgt cgacaaagcg gaaggcaatg cggcagcggc atttgccgtc aatgcttccg
    85141 ccgtttacaa ccttgccttg gcagcacatc gcgcccatgc ccgattcatc cacatctcaa
    85201 ccgactatat ctttgacggt aaagggaaaa ggccctatca ggaaagcgac tttaccaatc
    85261 cttccaatgt atacggacaa tccaaaaccg caggcgagct gctcgcactg tctgccaatc
    85321 ccgacagcct tatcctgcgg acttcttggc tgtttagcga atacggggac aactttatcc
    85381 gcacgatgct gaaccttgcg cgggaacgtt ccccgctgtc cgccgtccac aaccaaatcg
    85441 gctgcccgac ctatgccggc gacttgtccg ccgccatcat ccgcctgttg cagcagtcca
    85501 atcccgttcg cggcatttac cactacgtcg gcggcaaatc cgtatcctgg tacgaatttg
    85561 cccaacatat tttccaagcg gcatcgcaac agcagacatc cttccccgtt cccgaattga
    85621 ctgccgtttc agacaaggaa tatccgaccg ccgcccccag gcccgcatac agcattttgg
    85681 actgccgcaa aatcgaaaac gacttcggca tcaaaccgtc agactggcaa aaagcccttg
    85741 cgcaggtcgt ttccaagctg ctctgatgcc gcccgcctct ccgtttccgc cgtcaagcac
    85801 cgccttggcg gttttcttat atagtggatt aacaaaaacc agtactgcgt tgcctcgcct
    85861 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
    85921 tataaaatct accgattaca caaacacata tcatctttac acaatcatgc ttccatcaac
    85981 agtaaaacat gatatgattg ccaacaataa catctcacaa taaattttct aatttttatt
    86041 gaaaaaatca ataaataaga attctcccaa ccgacaaata aaataaagaa agggtcaata
    86101 tgcaacaccg aagaagactg gcaatttacc aggcatccaa acgtgcttcc tttactggca
    86161 ggtcatccgc cccgcaaaaa cgtaaagaac gttgatttga aaaaaatgcc gcctgaagtc
    86221 ctgcttcaga cggcattttt ttaccgttcg agaaactgtt tcaacctgtc ctcatccaaa
    86281 aaccagtggc agatttcctc atctcccgcc aacaaaacgg gaaccagctc attgtatttt
    86341 tcttccaaaa caggattttc atccacatcg accacttcca gcccgaaccc gtattcattt
    86401 tgaaaaggtt tgagtgcatc gcgcattttg tggcacaagc tgcaatattc acgaaacatc
    86461 aaggtcaatt tcatccgcgt ttccttatct gtcaatttgc acacgccaaa gccttagacg
    86521 cagcagaatc atggtctatt tgggaaaaaa caatgttttc aaggaagatg atactcaagt
    86581 cctgccaaaa acagcaaaaa tgccgtctga acagttcaga cggcatttcc aaaaccgttt
    86641 tacgcttgaa cattgatacc cgccgtaacc gtcggttgcg cattgtgcgc cgtcagaaac
    86701 gaagcggcaa gctccccaca gcagccgcag cagctcgcta cgcccaattg cgactgcaaa
    86761 tcgcccattg tggtcgcgcc ggcggcgatg gtttccttga tttgatggtc ggtaacggca
    86821 ttgcagatgc agacaaacat tttgtgctcc gtgtgttctc aaactatcgt accgatagcg
    86881 gcttttatta tcgtatgcga atataaataa aaacggttcg cattgcaagg tcggtataca
    86941 cggtttgtct tggtaatttt ttatcaagtt tgcattttcg attgatttct tattaaacca
    87001 aagtagaagc ctcagtttcg ggaatggtgt tccttcacta caccctgcaa ccatacgttg
    87061 gcaacatggg tataaatctg cgtcgtattc aaatcggcat gtcccaacat atcctgaacc
    87121 acgcgcaaat ccaagccgtg ccgcaccaga tgcgtggcaa aggcgtggcg caggctgtgc
    87181 gggctgatgt gcccgatgcc tgcctgactt gcatattctt tgacaatcat ccatgccaac
    87241 tgacgggaaa tgcccgtctt tttctgactg acaaacaatg cgtcgcaatt cctgcctttc
    87301 agcagaagtg ggcgtgcctc cgtataatag cgttccaccc aatacgccga ctcctgcccc
    87361 atcgggacca tcctctgctt atcacccttt cccagcgcgg taatacagcc cctgtccaaa
    87421 tccacattgc cgaagttcag cccgaccgcc tcgctgacgc gcaagccggt cgcgtacatc
    87481 aattcgagca aagccttgtc ccgcaaaccg tgcggcgtgt cggtatccgg ggcggcaagc
    87541 agtcgggaaa tctgctgctc ggtgatcagg gtcggaatat tcttgtcgat tttgggcggt
    87601 ttcagcaaac gggtgggatt gtccgtcctt atgccttcac gctccatcca tatatacagg
    87661 cgtttgcatg ccgataatgc gcgcgcctgc gaactccgtt gctctccgtc aacataaacc
    87721 gccgccgcca aatccgcttc gtccgcatcc ttcagcattc tgcccgattg ggacaggcgg
    87781 cgggcgattt tttccaaatc gcgccggtaa ccgtttaaag tattctgact gagccgcctg
    87841 tccaaccaca gcgtttcaag cagcctgtcg atcaaacctt cttccatacc gttccaaaca
    87901 aatgccgtct gaatcttctt cagacggcat ggttcacatt atcgggaaag cgtttccaat
    87961 acttcctgcg cgtgacccgc cactttgact ttccgccatt catgggcgat ttctccatcc
    88021 ttattcaaga cgaacgtact gcgctcgata cctaacgact ctttcccgta cagtttcttc
    88081 aatttgatga catcaaacag gcggcacact gtttcatcct tgtcgctcaa cagctcgaac
    88141 cggaaaccct gcttggcgca aaaattctga tgcgccttta cgccgtcgcg ggaaataccg
    88201 accacggtat aacccaatgc ctcaaactgt tccaaacgcg cattgaaatc caagccttcc
    88261 gtcgtacagc ccagcgtact gtctttcgga taaaaataca cgaccaaagg cagatgttct
    88321 gccgaatgaa aatccgcacc gctgctcgaa ggcagggtaa attcatattt cacatccata
    88381 gtcctactcc cgatattccc attattcaaa acggcacgca gacgcaccgc cgcaattgcc
    88441 aaaccaaccc cgattctacc gccccaaagg acaaggattc aaccgccgga aacatccaaa
    88501 ccgacacacg acggcatgaa aaatatccat gtcaaaccac aaaatatgtt ccgatttaaa
    88561 aacagaatgt tataaaaccc aatccccaaa acacaacaag accgcccgcc tacgggcagt
    88621 ctcctgtcag acgacatact ttacagatgg ctgttttttc aacaaaataa cgccaatact
    88681 caaaaatatg gaatcaaaaa tgtccatcca tactctgaaa cgcctgccct catcgctgct
    88741 gctcggtctc tgcctttccc tgccgtcagc ccaccttttt gccgacaacg acattttagg
    88801 gcaattttta gaacagaaca tgcttacctc ctccgatccg atagaaatat tcgccgaaag
    88861 cacgatacac cccaccaaca cccaagccat tacaggcggt ctgattctct cctcacagtc
    88921 tgccctggtc gtcaacaaca aaaccggaca gatactgtat cagaaaaacg ccgacaggat
    88981 tatgcccatc gcctccattt ccaaactgat gagcgcgatg gtcgttttgg atgcaaactt
    89041 ggacatgaac gaaaccgtta ccattacgcc cgacgaaatc gaccgcatca aagggaccgg
    89101 cagccgtctt gccataggta cggcacttac acgcaaagaa ctgctgcacc tgagcctgat
    89161 gagcagcgaa aaccgcgcca cccatgcatt gggcagaacc taccccggcg gcatgggcgc
    89221 atttgtcgcc gccatgaacc gcaaagccca aagcctcggt atgtacggca gccgctttta
    89281 cgaaccgacc ggactcaact tccaaaacgt ttccaccgcc aaagacctga gccttatggt
    89341 caacgccgcc gcccaatatc cgcaaatccg caccaactcg acttccaact acgcctcggt
    89401 acagaccaaa aacgggcagc agaactacaa aaactccaat gcgctggtca gggaaggcat
    89461 gtggaacatc gaattgcaga aaaccggcta catacgcgaa gcaggcaggt ctatggttgt
    89521 caaagccaac attcaaaatc aacccgttac catcgtattg ctgaactcgc ccacatccgc
    89581 cacacgcgtc aacgacgccc gcaaaatcga atcgtggatg ctgcagcaac gctcctgaca
    89641 tacaaatgcc cggcggaaaa ccgcccaaac acaaaaaaaa ttggtaattt ttcctattca
    89701 aggctacaat tcgacacgca cagggcatct gcccgatttc acaatcataa tagcggagtt
    89761 aaaaatatga cagctaaagg acaaatgttg caagatccct tcctgaacgc cctgcgtaaa
    89821 gagcatgttc cggtttcgat ttacttagtt aacggtatca aattgcaagg tcaggttgag
    89881 tctttcgatc aatacgttgt tctcctgaga aacacttccg tcacccaaat ggtttacaaa
    89941 cacgccattt ccaccatcgt accggcacgc tccgtcaacc tacaacatga aaacagaccc
    90001 caagccgcac cgacttcgac cctcgtccaa gtggaaaccg tccagcagcc tgccgaataa
    90061 tccgcacgaa gcatgacgtg tcatatcttt caatacctta ccggacaacg gtaaggtatt
    90121 tttattttca gacagcattt aaaaatgtta ttgcaaaaca tccttccatt cgcccattgc
    90181 cttttgcgga aggcacttcc cgaaggcggc aatgctttgg acggcaccgc cggcaacgga
    90241 cacgacaccc ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac
    90301 atccagccgc aagccctgag ccgcactcga tgccatctga aagaagcgag atacgagaat
    90361 gtgcaactca tcttagacgg acacgaaaac ctgaaacaat atgtttcaga accactaaat
    90421 gccgccattt tcaactttgg ctggctgccc ggcggagata aaagccttac cacccatacg
    90481 gaaaccagca ttcccgccct ttgcgcaacc ctctccctgc tgaaagaagg cggtatgctt
    90541 attgccgtcc tctatccggg acacgaaaac ggcaaacagg aggcagaagc aatcgaacaa
    90601 tgggcaaaaa acctgcctca agaacagttt gccgttttga gttacggctt taccaaccgg
    90661 aaaaacaacc cgccctatct gttggcattt gaaaagttac gtcaaaaata aatgtttgcg
    90721 gtaaaataag ccgattctaa cccttatttt gatacgagcc gaccatgcag tatctgattg
    90781 tcaaatacag ccatcaaatc ttcgttacca tcaccatttt ggtattcaac atccgtgttt
    90841 tcctactttg gaaaaatcca aaaaagccct tggtcggctt ttggaaggca ctgccccacc
    90901 ttaacgacac catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc
    90961 cgttcaacgc gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg
    91021 gtatgatgat gatgcgcgcc cgtccgcgtt cgaccaagtt ctacaccgtt tacctgctcg
    91081 ccatgtgttg cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg cctttctgaa
    91141 acaccgttat gaacaacaga cattttgccg tcatcgccct gggcagtaat cttgaaaacc
    91201 ctgcccaaca ggtacgcgcc gcattggaca cgctgtcgtc ccatcccgat attcggcttc
    91261 atcaaacctc gtccctgtat gccaccgccc ctgtcggcta tgacgaccag cccgactttg
    91321 tcaatgccgt ctgcaccgtt tccaccgata tggacggcat ctcgctgctc tccttgctca
    91381 accgtatcga ggcggatttc gggcgcaaac gcagtttccg aaacgcgccg cgcacattgg
    91441 atttggacat tatcgacttt gacggcatct ccagcgacga cccccgcctc accctgccgc
    91501 atccgcgcgc acacgaacgc agtttcgtca tacgcccttt ggcagaaatc ctccctgatt
    91561 ttgttttagg caaacacgga aaagttgccg aattgtcaaa acaactgggc aatcaaggta
    91621 tccgtctttt accggacagg taattccgca cacagatgcc gtctgaatgc cggtttcaga
    91681 cggcattttt accctgccgc caatctgtac gcaaaaaaaa tcgccccttg aaaaaggggc
    91741 gcaaaaggaa cacaaaccac taccaaaact ttaaatctgc aacactgcct gccgcatact
    91801 gtatccgaca ggatataaag ccctcactaa atcgtttcga gaaatccaaa cttcttcatc
    91861 gccgacagaa aatctgcctt tctccggcac cagctccaac aaaaacggtt gaaccgccgt
    91921 atgcagcctg tccttacacc gccccagctt tctggacaac gcggacaagg cacgcttata
    91981 gacactatcc ttgcggtcaa gttcccgcgc actgccgacc cagctcaaac gaaggttgcg
    92041 gctttcatcc tcaatcagca aaatgcctgt tttgacttcc cccacctcgg gctcacatga
    92101 aagcgcaata taatatttgc cgtcgatgtt gacatacgcc gcttcatgca cctgacagtt
    92161 gcagccggaa gtggcaagca gcgaataaac agagtcccga tgcagtttca aaatatcaag
    92221 ggaaatctgt ttgaaaatac tttccggcat ttttccctcc tttcgtctgc tgcgttgttt
    92281 actggattga ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt
    92341 ttttaaaaaa tttataaatt attgatggat atgtgaatta attttaagat ttaaaatctc
    92401 tttctcaaaa aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag
    92461 ccagtccgtt taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg
    92521 ataatccacg ctgtcaaagc ccgcatccag catcatctgt ttcaaagttt cctgatcggg
    92581 gtgcatacgg atggattcgg cgagatattg ataactctcc gcatctttgg caatcagttt
    92641 gcccatgacc ggcagcagct tgaacgaata gaaatcatac gcgccttcca aaggtttgta
    92701 gattttggaa aactccaata ccagcaatgt cccgccgggt ttcaaaacac ggtacatctc
    92761 tttcagcgcg gcatctttat gcgtcatatt ccgcaaaccg aacgccacgg aaaccaagtt
    92821 gaaataattg tcagggaaag gcagtttttc cgcatcggca agcgacacgg gcaaaatcat
    92881 gccttcgttc aaaagacggt cgcgcccgac ggtcagcata gaggaattaa tatcggtcag
    92941 ccaaacctcg ccttccttgc cgacccgttt cgcccaaccg cgcgacaaat cgcccgtacc
    93001 gccggcaatg tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg
    93061 cttccacacc ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc
    93121 gacggagtgg aacacttccg ccacttttcc ggctttctca tcttcgttga ccgtactgaa
    93181 tccgaaatgc gttttctgtc cgcccatatt ctaaccacct ccctgatgcc gccctacggc
    93241 atcccaaact taacaatctg attgcttttc aaaacgggaa gcacccgccg ccgccaattt
    93301 gtccaaatat tcctgccaca tcgcatcgta tccgtatgcc agtctgtgca gatacgccca
    93361 atcgtaaaga ccgctgtcgt gcccgtctga aaaactgatt ttcagcgcgt actgtccgac
    93421 aggctgcaaa tccgcgattt ggacatccgc cttgccggtc tgcaaaacat cctgtcccac
    93481 gccgtgtccg cgcacttccg cactcggcga atagacgcgt aaaaattcgg caggcagatt
    93541 tttaggttcg tccccataag tcaaaaccaa cacggtacgg tttttcaaca aacggatttc
    93601 aacaggaata ttattcaaaa aacacctcgg ttcggcatac aatatccggc aggcgcaggc
    93661 gtgccttcaa acggcgcgta ttttaacagc tttatcccga taccgatatt caaacccaca
    93721 ctttaaacgg cattgatatg gacaatctcg tatgggacgg cgttcccgac atccgcacac
    93781 tcgaccaaac catccgcaaa cacgcacacc cgctcaacct gattgtctgc ctccccgata
    93841 atcagattcc cgattttcaa accgcacaag atgcttcgga cgcggaatgc cgtctgaagc
    93901 accgtttgga tcaggcaacg cagtgcctcc agttcgacag catcaacctc atcgaacaca
    93961 tcctgcccga tgtccgcttc tggctggttc ccccttcacg cacccgccgc ctgcacgaac
    94021 acttccacca catttcctgg cagaccgaag ccatcccgca aaccgaaagc aaacccgaca
    94081 aaccctggtt tgcacttcca caaacatccg aacggcaaaa accggaacac atcctcgtta
    94141 tcggcgcggg catatccggc gcggcaaccg cccacgcctt agcatcacac ggcatttccg
    94201 ttaccgtatt ggaagcccga aaagccgccc aagccgccag cggcaaccgc caagggctgc
    94261 tctacgccaa aatctcgccg cacgacaccg aacaaaccga actgctgctt gccggctacg
    94321 gctacaccaa acgcctgctc ggacacatcc tgcccgaatc cgaaacctgg ggcggcaacg
    94381 gcatcatcca cctcaattac agccgcaccg aacaacaacg caatcacgaa ttgggtttgc
    94441 aaaaacacca taaccacctc taccgcagca tcacgcaggc agaagccgaa aaaatcgccg
    94501 gtatcccgct gaacacgccc tacgccgaac cattatgcgg actgttttgg cagcacggcg
    94561 tatggctcaa tccacccgca ttcgtccgcg ccctcctccg ccatccgctg attggactac
    94621 acgaagacac accgttaacc ggtatttccc acgacggaga aaagtggatt gcaagcacgc
    94681 caaacggcat cttttctgcc acacacatcg tctactgcac cggcgcgcac agcccctgcc
    94741 tgcccgaaat caacctcgcc gccctgcccc tcaggcaaat acgcggacaa accggcctca
    94801 caccgtccac cccgttttcc gaacaactgc gttgcgccgt ttcaggcgaa agctacatca
    94861 gcccgtcgtg gcacggactg cactgctacg gcgcgagttt tattcccaac agcagccata
    94921 ccggatggaa cgaagccgaa gaagcctcaa accgccaagc attggcacac cttaaccccg
    94981 cccttgccga atcattgttt gccgccaacc caaaccccca aaaataccaa gggcacgccg
    95041 ccatacgctg cgacagcccc gaccaccttc ccctagtcgg cgcactcggc gacattgccg
    95101 ccatgcggca gacctacacc aaactcgcgc tggacaaaaa ctaccgcatc gacaccccat
    95161 gcccatacct gcctaatgcc tacgtcaaca ccgcccacgg cacacgcggg cttgccaccg
    95221 cccccatctg cgccgccgcc gttgcagccg aaatcctagg cttgccccat cccctctcaa
    95281 aacgcctgcg ccacgcccta caccccaacc gcaccatcat ccgcgccatc gtcagaagga
    95341 aggatctaac cccttaagcc tttgccgcat gccgtcccga ccacagaaaa tcatattcaa
    95401 ataaacatac caatcctgac ggtaaaatat cgaaaacacc cataacgttt tcttcccaaa
    95461 ataaggagta aggaatgcct aacaaattgc ctttgctgtc caagatgaat gttccgtaac
    95521 attcgaagac ttccttaacc ctgccagccg tttcggtttc tgtaccccct gttagataag
    95581 gaaggaatga aaaagccctg ttgattgtat gaagtaaagg gcgttaattt ttgttaatct
    95641 tcccttctca ggaactcaat atataaggat gttaattttt atttcccttc tcgaggact
//
LOCUS       DAWFIT010000002        50310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFIT010000002 DAWFIT010000000
VERSION     DAWFIT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 50310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.002"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..68)
                     /locus_tag="WGQ85_000102"
     CDS             complement(<1..68)
                     /locus_tag="WGQ85_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0749623.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGQ85_000103"
     CDS             complement(271..987)
                     /gene="trmB"
                     /locus_tag="WGQ85_000103"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0749624.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1109..1880)
                     /locus_tag="WGQ85_000104"
                     /pseudo
     CDS             complement(1109..1880)
                     /locus_tag="WGQ85_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(1913..2170)
                     /locus_tag="WGQ85_000105"
     CDS             complement(1913..2170)
                     /locus_tag="WGQ85_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749625.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAPRKP"
     gene            complement(2443..4329)
                     /gene="uvrC"
                     /locus_tag="WGQ85_000106"
     CDS             complement(2443..4329)
                     /gene="uvrC"
                     /locus_tag="WGQ85_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0749626.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(4384..6561)
                     /locus_tag="WGQ85_000107"
     CDS             complement(4384..6561)
                     /locus_tag="WGQ85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0749627.1"
                     /translation="MQQKVRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            6700..7650
                     /gene="trxB"
                     /locus_tag="WGQ85_000108"
     CDS             6700..7650
                     /gene="trxB"
                     /locus_tag="WGQ85_000108"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0749628.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGTELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            7806..8174
                     /gene="rpsF"
                     /locus_tag="WGQ85_000109"
     CDS             7806..8174
                     /gene="rpsF"
                     /locus_tag="WGQ85_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0749629.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8175..8477
                     /gene="priB"
                     /locus_tag="WGQ85_000110"
     CDS             8175..8477
                     /gene="priB"
                     /locus_tag="WGQ85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0749630.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            8484..8714
                     /locus_tag="WGQ85_000111"
     CDS             8484..8714
                     /locus_tag="WGQ85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0749631.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8731..9183
                     /locus_tag="WGQ85_000112"
     CDS             8731..9183
                     /locus_tag="WGQ85_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0749632.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            9197..9304
                     /locus_tag="WGQ85_000113"
     CDS             9197..9304
                     /locus_tag="WGQ85_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0749633.1"
                     /translation="MPSETLPIVGMWFQTVFVLFFVYSAKIGMVVFIVD"
     gene            complement(9971..10777)
                     /locus_tag="WGQ85_000114"
     CDS             complement(9971..10777)
                     /locus_tag="WGQ85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0749634.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(10778..11524)
                     /gene="pssA"
                     /locus_tag="WGQ85_000115"
     CDS             complement(10778..11524)
                     /gene="pssA"
                     /locus_tag="WGQ85_000115"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0749635.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(11579..12001)
                     /locus_tag="WGQ85_000116"
                     /pseudo
     CDS             complement(11579..12001)
                     /locus_tag="WGQ85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12107..12590
                     /locus_tag="WGQ85_000117"
                     /pseudo
     CDS             12107..12590
                     /locus_tag="WGQ85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(12642..13853)
                     /locus_tag="WGQ85_000118"
     CDS             complement(12642..13853)
                     /locus_tag="WGQ85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0749636.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14076..16514
                     /locus_tag="WGQ85_000119"
     CDS             14076..16514
                     /locus_tag="WGQ85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0749637.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            16722..18032
                     /locus_tag="WGQ85_000120"
     CDS             16722..18032
                     /locus_tag="WGQ85_000120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0749638.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18128..18742
                     /gene="clpP"
                     /locus_tag="WGQ85_000121"
     CDS             18128..18742
                     /gene="clpP"
                     /locus_tag="WGQ85_000121"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0749639.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(18795..20060)
                     /gene="ispG"
                     /locus_tag="WGQ85_000122"
                     /gene_synonym="gcpE"
     CDS             complement(18795..20060)
                     /gene="ispG"
                     /locus_tag="WGQ85_000122"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0749640.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20076..20837)
                     /gene="pilW"
                     /locus_tag="WGQ85_000123"
     CDS             complement(20076..20837)
                     /gene="pilW"
                     /locus_tag="WGQ85_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0749641.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(20840..21934)
                     /gene="rlmN"
                     /locus_tag="WGQ85_000124"
     CDS             complement(20840..21934)
                     /gene="rlmN"
                     /locus_tag="WGQ85_000124"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0749642.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22078..22503)
                     /gene="ndk"
                     /locus_tag="WGQ85_000125"
     CDS             complement(22078..22503)
                     /gene="ndk"
                     /locus_tag="WGQ85_000125"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0749643.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(22562..23713)
                     /locus_tag="WGQ85_000126"
     CDS             complement(22562..23713)
                     /locus_tag="WGQ85_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0749644.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(23897..24000)
                     /locus_tag="WGQ85_000127"
                     /pseudo
     CDS             complement(23897..24000)
                     /locus_tag="WGQ85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24107..24934)
                     /gene="fghA"
                     /locus_tag="WGQ85_000128"
     CDS             complement(24107..24934)
                     /gene="fghA"
                     /locus_tag="WGQ85_000128"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0749645.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(24943..26052)
                     /locus_tag="WGQ85_000129"
     CDS             complement(24943..26052)
                     /locus_tag="WGQ85_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0749646.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGVGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            26200..26607
                     /locus_tag="WGQ85_000130"
     CDS             26200..26607
                     /locus_tag="WGQ85_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0749647.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(26958..27272)
                     /locus_tag="WGQ85_000131"
     CDS             complement(26958..27272)
                     /locus_tag="WGQ85_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0749648.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(27283..28968)
                     /gene="rpsA"
                     /locus_tag="WGQ85_000132"
     CDS             complement(27283..28968)
                     /gene="rpsA"
                     /locus_tag="WGQ85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0749649.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(29124..29780)
                     /locus_tag="WGQ85_000133"
     CDS             complement(29124..29780)
                     /locus_tag="WGQ85_000133"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0749650.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRDSLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(30024..30833)
                     /locus_tag="WGQ85_000134"
     CDS             complement(30024..30833)
                     /locus_tag="WGQ85_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0749651.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIYVFGNIPSILSYGPWKDVSVFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFVSNLF"
     gene            complement(30893..31336)
                     /locus_tag="WGQ85_000135"
     CDS             complement(30893..31336)
                     /locus_tag="WGQ85_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0749652.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            complement(31865..32557)
                     /locus_tag="WGQ85_000136"
     CDS             complement(31865..32557)
                     /locus_tag="WGQ85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0749653.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPAGETLCETLKNGVLLHD
                     ENETVYAADAVLESPSALLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            complement(32625..34601)
                     /locus_tag="WGQ85_000137"
     CDS             complement(32625..34601)
                     /locus_tag="WGQ85_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0749654.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            34779..35444
                     /locus_tag="WGQ85_000138"
     CDS             34779..35444
                     /locus_tag="WGQ85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0749655.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            35496..36323
                     /gene="mutM"
                     /locus_tag="WGQ85_000139"
     CDS             35496..36323
                     /gene="mutM"
                     /locus_tag="WGQ85_000139"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0749656.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            36411..37178
                     /locus_tag="WGQ85_000140"
     CDS             36411..37178
                     /locus_tag="WGQ85_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0749657.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(37490..37801)
                     /locus_tag="WGQ85_000141"
     CDS             complement(37490..37801)
                     /locus_tag="WGQ85_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0749658.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
     gene            38330..40609
                     /gene="nrdA"
                     /locus_tag="WGQ85_000142"
     CDS             38330..40609
                     /gene="nrdA"
                     /locus_tag="WGQ85_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0749659.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            40667..41680
                     /gene="dcm"
                     /locus_tag="WGQ85_000143"
     CDS             40667..41680
                     /gene="dcm"
                     /locus_tag="WGQ85_000143"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0749660.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            41673..42860
                     /locus_tag="WGQ85_000144"
     CDS             41673..42860
                     /locus_tag="WGQ85_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0749661.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            42921..44054
                     /gene="nrdB"
                     /locus_tag="WGQ85_000145"
     CDS             42921..44054
                     /gene="nrdB"
                     /locus_tag="WGQ85_000145"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0749662.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            44252..44542
                     /locus_tag="WGQ85_000146"
     CDS             44252..44542
                     /locus_tag="WGQ85_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0749663.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(44583..44861)
                     /gene="ftsB"
                     /locus_tag="WGQ85_000147"
     CDS             complement(44583..44861)
                     /gene="ftsB"
                     /locus_tag="WGQ85_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0749664.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(44875..46161)
                     /gene="eno"
                     /locus_tag="WGQ85_000148"
     CDS             complement(44875..46161)
                     /gene="eno"
                     /locus_tag="WGQ85_000148"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0749665.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            complement(46208..46648)
                     /locus_tag="WGQ85_000149"
     CDS             complement(46208..46648)
                     /locus_tag="WGQ85_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0749666.1"
                     /translation="MMQTFRKISRYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            complement(46663..47505)
                     /gene="kdsA"
                     /locus_tag="WGQ85_000150"
     CDS             complement(46663..47505)
                     /gene="kdsA"
                     /locus_tag="WGQ85_000150"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0749667.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFCVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(47527..47910)
                     /locus_tag="WGQ85_000151"
     CDS             complement(47527..47910)
                     /locus_tag="WGQ85_000151"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0749668.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(48059..>50310)
                     /gene="mfd"
                     /locus_tag="WGQ85_000152"
     CDS             complement(48059..>50310)
                     /gene="mfd"
                     /locus_tag="WGQ85_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0749669.1"
                     /translation="VSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQRAIA
                     VITESDLYQYVARSRKYHRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTM
                     DLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKA
                     AEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKD
                     LTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRF
                     ADFPVKVAGLSRFNNSKATKAALEGMADGTVDIIIGTHKLVQDDIKFKNLGLVIIDEE
                     HRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTF
                     VKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRE
                     RELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGR
                     SHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSG
                     EMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHER
                     LVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLAAKELGIDAIDAA
                     GESVTVTFGKNHNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLK
                     TLQNRCLPK"
BASE COUNT        11956 a        11868 c        13621 g        12865 t
ORIGIN      
        1 ggagcagttc tttctgtact tttttcttta atttgcagtg cgttatcttc atatttcgag
       61 ggtaacatat ctgctaatct agtacagccc caactttaaa accgatgtgc aggttaaaac
      121 ctacaaggat gcgtgcggta tacacgcgtt tttgccgttc aaaccattgc tttgaacttt
      181 gtatcgaaac atcgttcgct gctttatctt gaaaaaatgc cgtctgtaag caaacatgct
      241 tcagacggca tttttttatc cggcggtttg ttatccgatc cgtttgaata ccaagtccca
      301 aacgccgtgt ccgaggcgtt tgccgcgcgc ttcgaatttg gtttcggggc ggtagtccgg
      361 ggtgggggcg tagtctgccg ccgtattttg caggctgtcg aagctgctga ggacttcaag
      421 catctgctgt gcatattctt cccagtctgt cgccaggtgg atatagccgc cggttttgag
      481 tttgggcagc agtttggcga tgaagggggc ttgtatcaga cggcgtttgt ggtggcgttt
      541 tttgtgccac gggtcgggga agaatatgtg gatgccgtcg agcgagccgt cttgcagcat
      601 attttcgaca acttctacgg cgtcgtgccg catcacgcgg atgttttcca aatggttttc
      661 ttctatgagt ttgagcagat tgcctacgcc gggaccgtgt acgtcgatgg caagaaagtc
      721 ggtttcgggc agcctgcggg cgatttctgc ggttgccgtc cccataccga agcctatttc
      781 gaggattttg ggtcggctgc ttccgaaacg ggcatcaaga tcggccggtg ttgcttggta
      841 gtcgatgccg aagctgcccc ataaggtatc gatggcgcgt tgctgcgcgg cggtcatgtg
      901 gctttggcgg aggacgaaac tgcggatgct gcgggggtgt tcggcttgcc agttgttttg
      961 tgtttggttt tcggcggtgt ctgtcataat gctttcgtgc gcttgtgcaa tcaggatgcc
     1021 gtctgaatgt gaaaggcctg ttcggacggc gggaaaggaa aggggcgatt atacctgttt
     1081 cgtttcggca gggtaggtgt aacgctgttc agacggcatt ttgccattgt tgcagcagtt
     1141 cttgcaggac gcttttttgc ccgtatccgt tgtttatggc ttcttgcagg tagcggcagg
     1201 ctttttcttt gtccggctcg acgccgtgtc cgttgtaatg gatgcaggcg aggttataaa
     1261 gtgccatgct gtctttttgc gcggcggctt tttcaaacca tttgcgtgct tcattgtagt
     1321 cggcggtcat accctgtccg aagtaataca tgctgcccag attggtttgg gctttgctgt
     1381 ggccgagttc tgcggcttct gtgtagagtt tgagtgcggc tgcatattcg ggcgcgcatt
     1441 gtaatccgta atgcagggct tgggcttttt gataaagccg ttctgccctt tgccgcctgt
     1501 tgaaaccagt gaacggcttc atcaggagcc gtaggtagga tgagctcggc aagcggggtg
     1561 taggcgggaa tgtatccttg ttgtgcggcg gcgcgcaaat gattgacggc ctgtgccgtg
     1621 tcgactttcg tcccttgtcc gaaacggtat tgcagcccga gctgccagtg tgccgccgcc
     1681 aagccttgtg ccgccgcttt gcggtagagc aggtgcgccg tttcaaaatc cggcggcgtg
     1741 ccgtaccggc agtattgggc aagcaggtat tgcccgtggg ggttgttgtt ttttgcgctt
     1801 gtttttgccc atttcagggc ttcttcaaaa tgtcgggacg catacagaag gtacatcagg
     1861 cggacttgtg cttcaggcag tccttcctct gccaacggtc tgtaaagcgg tatcagggtt
     1921 tccggggggc cagattgtaa cgcgcatagg gatgcccctg ttcggcggcg atgcggagca
     1981 tttccatacc ttgttcgcgg tcttggtagg ggctgtccgc gtccatcagg tgttttgcca
     2041 actcgaatgc ggcggcggga tgacctgccg agaacgcttc tgtcagcagt cggcggactt
     2101 cggtaaagtt atattgtttt tgagacaggc attctaaggc ctgccgatag ggtaagtcgg
     2161 gattgttcat gtcagatcgt tttccgtttt gtcgaatcat cgttattgtg ttatgaagtt
     2221 aggtttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     2281 agtacgaaac cgattcactt ggtgcttgag caccttagag aatcgttctc tttgagctaa
     2341 ggcgaggcaa cgccgtactg gtttaaattt aatccactat atatcggtat accgacctgc
     2401 tgtatcatat tttttacaga cggtttttgc ttgggcagca tactaatgca gatgctcgta
     2461 aatggtttcc gccaatgcct tgctgatgcc ttccactttt tccaagtcct cgcggctggc
     2521 ggcaatcacg ccgcgcagac cgccgaagcg ggtgagcagg gcttggcggc gtttgctgcc
     2581 tacgccgggg atgtcgctta aggaggaggt aacgcgggct ttgtcgcgtt ttttgcggtg
     2641 gccggtaatg gcgaaacggt gcgattcatc gcgtacggtt tgcaatagat gcaaggccgg
     2701 gctgttgggc ggcaggcgga agacttcgcc ggtaaaaggc agtatgagtt cttccatacc
     2761 ggctttgcgt tcggggcctt tggcgatacc gaccaaagga atgtgcagcc cgagttcttc
     2821 ccataccgat acggcgatgc cgatttgccc tttgccgccg tcaattaaca cggcatccgg
     2881 ccatttgacg gtctcgccgt tggcttcggc ttcctgcatt ttgccgtaac ggcgcgtcaa
     2941 cacttcgcgc atggcggcgt agtcgtcgcc gggtttggcg gtcgtgatgt tgtagcggcg
     3001 gtattgcgag ggctggatgt tttgctcatc gtacacgacg caggaggcga tggtggcttc
     3061 gccttgggtg tggctgatgt cgaagcattc gaggcggttg aggccgtctg aatccatacc
     3121 gaggattttg gcgagttcgt cgatgcggtg ttgctggttg ctttgttgca ggcggcgttg
     3181 tgcaatcgcc atttgtgcgt tttgttccgc cattttcaac caaactttgc gttcgcctat
     3241 ggttttggtt acgaactgca tttgtttgcc gtgttcgcct tccaaagcct ctttcagcgc
     3301 atcggggacg gggaagttgt tgatgataat gtcgggtttg cttttgccca gatagtgttg
     3361 ggcgacgaag gcttcggcat agtcttgccc gtttggctcg ggatcgtttt tggtgtcggg
     3421 gaagaaactt ttgtctccga cgtgccgccc accgcgtatg ctgacccagt gtacgcagac
     3481 gatgccgtct gaaaccgcca gcgcgagcaa atcgatatcg ttggggttgt tcgggttttt
     3541 gctgtcgatg aactggttgc tctgcataat gccgagtgct tggatttggt cgcggtagcg
     3601 ggcggcttct tcaaattgca gattcgcggc ggcagtttgc attttgtgtt gcagggtgcg
     3661 ggtcagttcg tcggttttgc cgttgagaaa agtggcggct tgacgcacgc tgtcgcggta
     3721 gtcttcttcg ctgatgtgtc cgacgcaggg cgcggtgcag cgtttgattt gatacagcag
     3781 gcaagggcgg tcacgatgct cgaatacgct gtcttcgcag gtacgcagca taaagacttt
     3841 ttgcaacact tgaatgctgt cgcgcacggc gttgctgttg ggatacgggc cgaagtattg
     3901 gtttggcttt ttcagcgtgc cgcggtaata cgccatctgc ggatattgat ggccgctgag
     3961 catcaaataa ggatagcttt tgtcgtcgcg gaaaaggata ttgtatttcg gcgacaaggc
     4021 tttgatgagg ttgttttcga gaatcagggc ttcggcttcg gaacgcgtga tggtggtttc
     4081 gatatgatga acctgtttta ccatcaatgc gatgcgcggc gaatggtcgt ttttttggaa
     4141 atagccggac acgcgccgct tgaggttgac cgctttgccg acgtataaga ctttgccgcc
     4201 ttcgtcaaaa aaacggtaca cgcccggcag cttgggcagg tttttgagga agagcgggat
     4261 atcgaattgt tccggagaac ggttttctgt attcatatca attatttgaa aaacggcttc
     4321 agacggcata tcggcagcgg cagccttatc cgttcggaaa agctgtcggg tggttatatg
     4381 cggtcaatcg atttttaccc gtttcaggcg caaggcgttg ctcaacacgg aaaccgagct
     4441 tgccgccatt gccgcgcctg cgatgacagg gtttaaaaaa ccgagcgcgg caagcggaat
     4501 gcccaaaata ttgtagaaga aggcgaaaaa caggttttgc ttgatgtttc tcaacgtcgc
     4561 ccgcgatacc gatagggcat cggcgagttg gttgaccgaa tgttgcatca gcgtggcgga
     4621 tgcggtgtgt tcggcaacgt ccgcgccgcc tttcatggcg aagctaacgt tggcggcggc
     4681 gagcgcaggt gcgtcgttga tgccgtctcc gaccatcgcc acggttttgc cggcggcttt
     4741 gagtttctgc acttcggcgg ctttgtcgcg cggactcata ttaccgaagg cgtgtgcgat
     4801 gcccagttgt ttggcgacgt attcgaccgt gccttggttg tcgccgctca taatatagac
     4861 atcgatattg tgttttttca gacggcctat ggcttcggcg gtatcggctt tcaacgcgtc
     4921 ggcaagtgcg aatgcgccga tgggtttgtt atcgactgag accgcaacaa tgcttgcaat
     4981 atcccaaacg ccgtctaaaa acttcggcaa ggccagttcg gcaaattcgg ctttgcctgc
     5041 tttcaccaaa cccacgcctt ccacttcggc ggtaatgcct gcgccgacaa cggtttgtgc
     5101 gttttgtgcg gcgggaatgt ccaaaccgcg cgcttgggcg gcggagacga tggcacgggc
     5161 gagcggatgg gcggcgtttt gttcgacggc ggcggcgatg cggtacaaag cgtcttcgtc
     5221 aaagccgctg tcgggaacgc aataaacggc ggcaacctgc gggctgcctt cggtcagcgt
     5281 accggttttg tccaacacga cggcatcgac gtgggcggct tcctccattg ctgccgcgtc
     5341 tttaaaccaa ataccgtgtt tcaccgcttt gccaatgccg accataatcg cggcgggtgt
     5401 agccagaccg agcgcacacg ggcaggcaat caccaaaacg gcgacggcgt gcatcagcgc
     5461 aaccgtccaa tcgcccttaa tcagccaagt aacaataaaa gtcaacaacg cgatgcccac
     5521 gacggcaggc acgaacaccg ccgccgcctt gtccgccacg cgcgcaatcg gtgctttact
     5581 gccttgcgct tcagagagtg cgttcatcat gtcgccgagc agggtttggc tgccgagctg
     5641 cgcggcgcgg tacaccacgc tgccttcagt catcagcgcg cccgccaata ctttgccgcc
     5701 tgcctttttc tcttcgggat tggattcgcc ggtaagatgg ctttcgtccg cccagccgct
     5761 gccgctttct atgatgccgt cggcggcaat gcgttcgccg tgattggcgc ggattaggtc
     5821 gccgatttgc acttggtcga tgggtagctg ccgccattcg ccatcgcgtt gcacgttgac
     5881 ttgggttggc gtgagtttga gcagcaagcc caagctgttc aggctggatt ttttggtgcg
     5941 gtgttccaaa aatttaccca gtgacacaaa accaatcacc attatgccta cttcaaaata
     6001 cacatgcgcc ataccgtacg ccgcgtgcgg gctgaaaaac agcatataga cggaatacag
     6061 gtaaatcgag accgtgccga tggtaacgag tacgtccata ttcgccagcc cgcctttaat
     6121 gctcgcccac gcgcttttgt aaaatggcac cgccagccaa agctgcacca cactcgccaa
     6181 tgcaaactgc aacaagggcg acaacatcca atcatgccgt gtccaattca gcccttttag
     6241 catcatccct accataccga taaggaacgg gatattgatg gccagcaaaa gccacaacct
     6301 ccagccgata tggtgttctg cttcgggttg cggcaatgta tcttccgttt tttccttcgc
     6361 gccgtaaccg gttttctcaa tgattttggc aatgtcggct actgaggttt tgctgtcgtc
     6421 aaacactacc tgcgcctctt cgctggcgaa gtttaccccc gccgattcga caaaatcttt
     6481 tttgttcaac actttttcaa tgcgcgaagc acatgcctgg caggtcatgc cttcgatttg
     6541 gaaacggact ttttgttgca taagcgtgat tcctgctttg atattcagac ggcggtcaag
     6601 gctgcgtgat gccgtctgaa cgccggtatg gtcgaaattt ctcaaacgcg ccgataattc
     6661 gagtagaatc cgaaaacttg acacagcaaa ggaaaaacaa tgtcccaaca ccgcaaactg
     6721 attattttgg gttccggccc cgccggatac accgccgccg tctatgccgc gcgtgccaat
     6781 ttaaaccccg tcattattac aggtatcgcg caaggcgggc aactgatgac caccaccgaa
     6841 gtggacaact ggcctgccga tgccgacggc gtgcaaggga cggaattgat ggcgcggttt
     6901 ctcgcccacg ccgaacgttt cggaacggaa atcatttttg accaaatcaa cgccgtcgat
     6961 ctgcaaaaac gcccgtttac actcaaaggc gatatgggcg agtacacttg cgatgccctg
     7021 attgtcgcaa caggcgcgtc cgccaaatac ctcggtttgc cgagtgagga agcgtttgca
     7081 ggaaaaggcg tttccgcttg tgccacctgc gacggtttct tctacaaaaa ccaagatgtt
     7141 gccgtagttg gcggcggcaa tacggcagtt gaagaggcac tctaccttgc caatatcgcc
     7201 aaaaccgtta ccctgatcca ccgccgcagc gagttccgtg ccgaaaaaat catgatcgac
     7261 aaactgatga aacgcgtgga agagggcaaa atcatcctca agctggaaag caacctgcaa
     7321 gaagtactgg gcgacgatcg gggcgtaaac ggcgcattat taaaaaacaa cgacggttct
     7381 gagcaacaaa ttgccgtcag cggcattttt atcgccatcg ggcacaagcc gaataccgat
     7441 attttcaaag gtcagttgga aatggacgaa gccggttacc tgaaaaccaa aggcggcacg
     7501 gcggacaatg tcggcgcaac caatatcgaa ggcgtatggg cggcgggcga cgtaaaagac
     7561 catacctacc gtcaggcgat taccagcgcg gcttccggct gtcaggcggc tttggacgcg
     7621 gaacgctggc tgggcagcca aaacatttga atgccgtctg aaaccgaaat tttcggcgga
     7681 atgcaaattt ttcgcaccaa atccttgatt ccgtcggcaa accccaatat aatgcctgct
     7741 ctttgaacct tgccttctgc tttcgcatgg cagaaggctg tttttaccat ccataaggag
     7801 ataacatgcg tcattacgag atcgtgttta tcgttcatcc tgatcaaagc gagcaagtgc
     7861 ctgctatggt tgaacgttac aaaaccatga ttgctgaagc aaacggtaag attcaccgtt
     7921 tggaagattg gggtcgccgc caactggctt acccgattaa caaaatccat aaagcccatt
     7981 acgttttgat gaacatcgaa accactcccg aagtggttga agagctggaa accgcattcc
     8041 gcttcaatga tgccgtattg cgccatctaa ccatcaaaac caaacacgcc gttaccgaag
     8101 catcccctat gttgggtggt gaaaaggcta agaacctgtt gagcggcgcg tctgaagaag
     8161 cggtcgccca ataattggga ttcaataatc ttgtttcgct tgccgtgtta attgaaaagg
     8221 ttttccctat tcgatatacg cctgccggaa tccctgtttt agatattatt ttaaagcacg
     8281 aatcgtggca ggaggaaaac gggcagcaat gccttgtcca attggaaatt ccggcacgga
     8341 ttttaggcag gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt cacgtcgaag
     8401 gttttttagc tcaaaaaagc agacgttccc ttatgccgat gctcaggata caaaatattc
     8461 aagaatataa aggttaaacg acaatggctc gtcaatcatt caaacgtaga aaattctgcc
     8521 gtttcacggc tgaaaaaatc caagaagtcg attacaaaca agttgatttg ctgaaagact
     8581 ttatctctga aaacggtaaa atcattcctg cacgcatcac aggaacgaag gcattctacc
     8641 aacgccaatt ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg ccttacaccg
     8701 accaacacaa ataattttgg agattgaatc atgcaaatta ttctgttaga aaaaatcggc
     8761 ggtctgggca acttgggcga catcgtaacc gttaaaaacg gctacgcccg caactttcta
     8821 attcccgcag gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt tgaagcacgc
     8881 cgtgccgaat tggaagccaa acaggctgaa attttggcag atgcccgagt ccgtcaggaa
     8941 aaattggacg gtcaaaccat taccgttgca caaaaagccg gtgtggacgg tcgcctgttc
     9001 ggttccgtca ccaatgctga tattgcggct gcaatcgttg ctgccggcat cgaagccgtg
     9061 aaagcaaatg tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta cgaagtggaa
     9121 gtggctttgc acactgatgc cgttgctaaa attaccgttg ccgtcgttgc cgcaaccgag
     9181 taattgaaat tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt ggtttcagac
     9241 ggtatttgtt ttgtttttcg tatattctgc aaaaatcggg atggttgttt ttatagtgga
     9301 ttaaatttaa accagtacag cgtacctggc ttcaatatac tcaataggat aggtctcttc
     9361 ctaaccgaaa aaataattgt cttttccctt gcaaataatt gaaatcaaca gattactaaa
     9421 acacagccaa aatattcaat tgacaaaaac ttttctgatt ttgagaaatc tgtgaaaatc
     9481 aggggattat ttattttgaa aatggtatcg ggcataaaat ttggctgtgt ttcaatatgg
     9541 tgctgataac atactttgtt tcaaaatgtt caataggata acttcctgat tgaccgaaaa
     9601 ataatggcct tttcccttgc aagcagttga aatcaacaga ttgtcaaaat acatcctaaa
     9661 aatttaataa cttatatata tgattgcaaa ggttacggct tgtatgccgg tcattatggc
     9721 ggaaataccg tttgggaggc agccgcatcg cgtggaatcg gcacggcagg cgatgcgccg
     9781 atgccttcgg gcgtgaaggc ggggcaatcg gcagtttgga aaaaggcaat ggaatctgtg
     9841 ggcagaagaa gttaagggta aaagtcgggc gcacggtcag gttgtctgtc cttttgtcgg
     9901 aacaggagac tgggcagctt tgtcctgatt aattgcttgt gcaaacggta aaaaggtttt
     9961 ttcgtgtgtt ttaaagcagg ttgtacagca tttttccggc aatcaaaagc aacataatgc
    10021 cgaaggattt tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca ccgagcgggg
    10081 caaaggcaat ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg aagcccagtg
    10141 acccttcggg caatcctgca atattcaggc cgttgagcag atacgatatt gcgccggaga
    10201 gtgcaatcgg ccaggcaagg ccggatgatg tgccgatggc tttatgggcg gggaagccgc
    10261 agtggattaa gaaggggacg gaaagtgaac cgccgcctat gccgacccag ctcgacattg
    10321 tgccgaacag tgtggaaacc gcagtcagtc cgggcagtcc gggcagcggg cgggatgccg
    10381 tctgagggtc ggtatgcagt gttttgaatg cgacggcggt taaaaacagg atgaagaaaa
    10441 tttgaagccc gaacgctggg atatattttg cggagagtgc gccagcgaat acgccgaata
    10501 ccatacccgg catcatcgta aatacggttt tccagtcgac cgcctgtttt ttgtgctgcc
    10561 ccagcatact ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg acggcgaggt
    10621 gttgcgcgta aggatgttgt gccaaaccct gcaaatcaag cacccataaa acgacaggga
    10681 caatcagcgt gccgccgcct acgccgaaca ggccggcaat aaaacctgcc gcactgccta
    10741 cggcaagcag gattaagata atgtcccaat gccacattta atccgccttt ctgtattttt
    10801 tccagaatcg gcgtgccgcc ataatgtagc cggacaggct gtatccgaga aagaacagga
    10861 agaggacgag cgacggttcc caagtgacca gaagcagcag taagacggca agcagcattc
    10921 cgacaaaggg gacttgtctg cggatgttga tttctttaaa actccaaaaa gggatttgga
    10981 caatcatcga caggccggca aacagtgtga tgcccaatgc ccaccagtgg acggcgggga
    11041 atttttcgac gctgtggttg acccaaatca gcccgacaat cagcgcggcg gcagtcggac
    11101 tgggcacgcc gataaaccag cgtttgtcca ccttgccgat gagcgtgttg aacagcgcaa
    11161 ggcgcagggc ggcgcaggcg cagtagatga aggcgacgga ataaccgatt ttgccgaact
    11221 gccaaagctg ccatttgtag gcaatcagag cgggagcaac gccgaagctg accatatcgg
    11281 caaggctgtc gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc gccacgcgcc
    11341 cgtccatacc gtccagcaac atagagatga ataccgcgat ggcggcggtt tcataacgtc
    11401 cgtgcatgga ttgggtgatt gcgtaaaatg cggaaaacag cgcggcgatg gtaaaggagt
    11461 tgggcagcag gtagatactg ttttgacgca gggagcgttg cggggtgttg gtcggggttt
    11521 ccatagctga agtgtctcac ggacgtggag aatcgatagg ggattataac ccaaaccatt
    11581 atcgaacaat acgcccttta ggatgaagcc ctcggaggcg gtttgttttt ctgatggcgg
    11641 atgctttgcg gggcggtttg actctgcctg ccaccgggtg tgttgtttcg gatttttgcc
    11701 ggatggatat tgttgccggg catttctggg cttgccggaa ggtatcgggg ggagggcatc
    11761 ggaatatgcg gcgcaccgtt caaaccgggg cggcgtttcc ctattttcag ggggttgcag
    11821 gtttcaacaa attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg
    11881 ataaattata ggacaaggtt tttcgcccgc ggcaaggatt gcccgcaatg ccgtctgaaa
    11941 acaacggctt gccgctaagg cggcggtatg cggatttgcg ctgccgaaat cggtttgcca
    12001 tacccggcgg tgcaatctgc ggggagggat aagccgataa ggcgggactt atcctgtttt
    12061 ttgtttataa tgcaacagaa tttggacgaa ccgtcgggaa tttatcatga tgaaaaagat
    12121 actggccgta tcggcactat gcctgatgac tgcggcggca cgggctgccg atacttacgg
    12181 ctatctcgcc gtttggcaga atccgcagaa tgcaaacgat gttttgcagg ttaaaaccac
    12241 aaaagaagat tcgacgaaaa gcgaagcgtt tgccgagttg gaagctttct gcaaaggtca
    12301 ggacacgctt gcgggcattg ccgaagacga gccgaccgaa tgccggtcgg tcgtgtcgct
    12361 gaacaatacc tgtgtcgcgc tggcataccc gaaagccttg ggcgcgatgc gcgttgaaaa
    12421 cgccgttgtg attacttctc cgcgttttac gagcgtttat caggtcgcac tcaaccagtg
    12481 catcaaaaaa tacggcgcac agggacaatg cggcttggaa acagtgtatt gcacgtcttc
    12541 ttcttattac ggggggaact gtgcgctctt tgattcaaaa tctcaaataa aacgggaaat
    12601 gccgtctgaa agatgttcag acggcatttc tatatcgacg gtcaggattc tttcggatcg
    12661 ggcagcaggc tgttcaacat aatggcaagt acggcgcaca agcccacgcc ggcaaagctg
    12721 aagctgccca atttgagcgt catgccgccg atgcctgtgg tcagtaccga gctgacgatg
    12781 accaggtttt tcggcagcat caaatcgact ttggcatcaa tcagggtttt gatgcctaaa
    12841 gaagcaatcg tgccgaacag cagcagcata atgccgccca ttactggcat cggaatggaa
    12901 gccaaaaacg cattgaattt gccgaaaaac gccatgcaga cggcaaaaac cgccgcccaa
    12961 gtcatgatga cggggttgct gtttttggta atcatcaccg cacccgttac ttcgccgtag
    13021 gtcgtaaccg gcgggccgcc gatcagaccc gcaacgcata cgcccaaacc gtcgcctgca
    13081 agggttttgt ccaagcccgg gtctttcgta tagtctttcc ccgtcacatt gccgattgcc
    13141 atgatgccgc cgatgtgttc gatggcgggg gcgacggcaa cgggcagcat aaacagtgca
    13201 gcctgccagt tgacctgagg cgtttcaaaa tggggaacgg cgaaccaggg cgcgtgtgca
    13261 atgcttgccg tgtccaccag tcccatcagc agtgccaaaa cataacccga agcgacaccg
    13321 atcaagatag gaatcagctt catcatcctg ctgccgaaaa ccgatacgat ggcggtaacg
    13381 gcaaaggtaa agccggaaag aatcagcgaa tcggtatagt cgatgacctg tttgccgtcc
    13441 gcctgaccca ttgccatgct gcttgccgcc acggcaacag acagaccgat gaccatgatg
    13501 acggggccga tgacgaccgg aggcagcagt ttgtgtaccg ccgccagtcc gcgccaacgg
    13561 atcagcgcgg caaacacaaa atacataaag ccggcggcaa acagtccgaa catggtggaa
    13621 ggcagccccc attcgccgac ggagtagata atcggtgcga taaaggcaaa cgaagaacca
    13681 agaaaaatcg gcactttgcg tttggttgtg atttggaaca gcagcgttcc caagcctgcg
    13741 cccaaaagcg caagagccgg attcagaccg gtcagcaggg gaaccagcac cattgcgccg
    13801 aatgccacaa ataaaatctg cgcgccggaa acggcaagtt tcagttggtt cataccatct
    13861 tctttcaatc ggttcaaacg gcacggatta tactggtttg ccgagggtct gcgtaaaaat
    13921 acatatggcg ggcaacagat atgccgtctg aaaggcaatg tctgtctgtt gcaggatgaa
    13981 agcaggagaa atggagtata atccgacact ttccatattc aatccgcaag ccgtccggct
    14041 tccttatttc agacggcatt ttccggtaat acggcatgac agaaaaatcc cataaaaaaa
    14101 cagcaaaagg cagggcggga agcccgtccc caacgtccgc acgcaacaag aaagccgaca
    14161 acggcgcacg gggcaataaa gtttccgagc ggctcaaagc ggtcaaagag ttgcagaaaa
    14221 ccgaaaccaa aaaggcgcgt cccgaacatg tcgtcaacct tatcggcgac gcactgtggc
    14281 tgatgggttt ggcggcaacc ttgtatttgg cgatttccct gatcagtttc gatatgggcg
    14341 atccgtcttg gtcgcacagt tcgccggttg tggaagatgt cgccaattgg ggcggactgt
    14401 tcggcgcgta tgttgccgat gtcggctatt atcttttcgg ctggtcgttc tggtggtgga
    14461 tagcggctgc ctgcgtcatg ctgtataaaa attttcgtct gcacgcaaaa cagacggaaa
    14521 acgaggcata caaccacaaa atcgctgccg ccgcgctgtt tgtcctgacg gtcttcagcc
    14581 ccgtcttgga gtattttgtg ctgggcggaa aatatgccga ctccctgcct gtcggagcag
    14641 gcggtatggt cggcatacgc gtcggcgcag tgtttgcgtg gctgctgggg aaatcgggca
    14701 gcctgctgat tatcttggtt gttctgctgt tgtcgttgtc cctgctggtg cagatttcat
    14761 ggctggaatt tttgaacggt gcgggcaggg cggttcaaaa ccgcctgagt gccttatccg
    14821 gcaaggtcat ggctttggga aaacgccggc cgaataccaa aacagacggt gtcgataccc
    14881 aaaatacacg gcgcatggta aaagaagcca agaatattac ggccaaaccc gttgccttgc
    14941 ccgaaggcag cagcagcaac cgcaaatccg tcgcggtttc cgtcgcgccg ccgcccaaaa
    15001 ttcaggtttc tctgtttgaa gatgacgaac ctcggcaggc gggcgaatac cacaagccta
    15061 cattgaacct attgcggatt cctgacagcg aacccgtcag catcaatccc gccgaattgg
    15121 agcgcactgc cgaactgatc gaatccaaac tggcagaatt cggcatcggc gtacaagtcg
    15181 tatccgccac atccggtccc gtcatcacgc gctacgaaat cgaacccgcg caaggtgtta
    15241 aaggcagcca aattgttgcc ttgtcgaaag atttggcacg ctctatgtcg ctgcagtccg
    15301 tgcgtatcgt cgaaaccatc gcaggtaaaa acacgatggg catcgagttg cccaacgaca
    15361 aacgccaaga cgtgatgttg agcgaaatct tgtcctcgcc cgtgtttgcc ggagccaaat
    15421 ccaagctgac cgtcgcgctg ggcaaagaca ttgccggtac ccccgttgtc ggcgacttgg
    15481 cgaaaatgcc gcaccttttg gtcgccggta tgactggttc gggcaagtcc gtcggcgtga
    15541 acggcatgat tatgtctatg cttttcaaag ctacgcccga cgaagtccgc ttcattatga
    15601 tagacccgaa aatgctcgag ttgagcattt acgacggcat tccgcacctg ctctgtcccg
    15661 tcgtgaccga tatgcgcgaa gcagggcagg cgttgaactg gtgcgtcgcc gaaatggaaa
    15721 aacgctaccg cctgctttcc cacgccggtg tacgcaatct ggaaggcttc aaccaaaaag
    15781 tcgaagccgc aaaagcggca ggcaagccgc tgctcaatcc gttcagtctg aaccccgaca
    15841 accccgagcc tttggaaaaa ctgccgatga ttgtggtcgt tatcgacgaa cttgccgacc
    15901 tgatgatgac cgaacgcaaa gccgtcgagc agcaaatcgc ccgtctcgcc caaaaagcgc
    15961 gcgccgccgg tatccatatg attgtcgcca cccaacgtcc cagtgtcgat gtcgttaccg
    16021 gcctgattaa agccaacatc ccgacgcgta tggcgtttac cgtgcaaagc aaaatcgaca
    16081 gccgtaccat cctcgaccaa atgggcgcgg acgaactgct caaatatggc gattcgctgt
    16141 tccttcagcc cggcagtgcc gaaccgaccc gcctgcaagg cgcgtttgtt tccgatgatg
    16201 aagtccacca cgtcgtcgct ttcgtcaagg agcaggcacc ggcaaattat gtagaagggc
    16261 tgcttacagg cgaagcggca caggaaacgg caaatatcgt cagccccaat gcagacagtg
    16321 acgaattgtt cgatcaggct gtcgcctatg ttttggaaag caaaaaaacc tccatttcgt
    16381 ctttgcagcg gcagttgcgt atcggctata accgcgcggc aaacctgatg gaggcactgg
    16441 aaaatgcagg tgtcgtttct ccctccgatc ttaacggcag ccgtaaaatt ttggcgcaca
    16501 aggaccattt atagcccata tcgcaaaatg ccgtctgaac ggtggaattg gcgtttcaga
    16561 cggcatatta tgtttcaggc gaaacattgt gatatacttg ccagctaaaa tttccccttt
    16621 gcggcaatgc ggttcaaata tcgtaccgtt gcgctgtttg cttccccatg tagggaagaa
    16681 agtttatcat tttatcaaca caacaaattt aagggcttat gatgagcgta actgttgaaa
    16741 ctttagaaaa tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa atcaacgcag
    16801 aaaccgataa aaaactgaaa caaacccaac gccgtgcaaa aatcgacggt ttccgtccgg
    16861 gtaaagcacc tttaaaaatg attgcccaaa tgtacggtgc gagcgcacaa aacgacgtga
    16921 tcaacgagct ggtgcaacgc cgcttccacg atgttgccgt tgcccaagag ttgaaagtgg
    16981 caggcttccc ccgttttgaa ggcgttgaag aacaagacga taaagaatct ttcaaagttg
    17041 ccgccatttt tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca caagaagttg
    17101 aaaaagtaac cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa atcctgcgca
    17161 aacaacgcac ccgcttcaac catgtcgaac gcgaagcccg aaacggcgac cgcgtcatca
    17221 ttgactttga aggcaaaatc gacggcgaac cttttgccgg cggcgcatcc aaaaactacg
    17281 ccttcgtatt gggcgcaagt caaatgctgc ctgaatttga agccggcgta gtcggcatga
    17341 aggcgggcga aagtaaagac gttaccgtca atttccctga agactaccac ggtaaagacg
    17401 ttgccggtaa aactgccgtg ttcaccatta cgctgaacaa cgtttccgaa gcgactctgc
    17461 ctgaagtcga tgcagatttt gcaaaagcct tgggtattgc ggatggcgac gttgccaaaa
    17521 tgcgcgaaga agtgaagaaa aacgtaagcc gcgaagtgga acgccgcgta aacgaacaaa
    17581 ccaaagaatc cgtaatgaac gcgctgctca aagccgtaga gctgaaagca cctgttgctt
    17641 tggtcaatga agaagccgca cgcttggcaa acgaaatgaa acaaaatttt gttaaccaag
    17701 gtatggctga tgctgccaac ttggatctgc ctttggatat gttcaaagaa caagccgaac
    17761 gccgcgtgtc tttgggtctg attttggcca aactggttga tgaaaacaaa ctggaaccga
    17821 ctgaggagca aatcaaagcc gttgtcgcca acttcgcaga aagctacgaa gatcctcaag
    17881 aagtaatcga ctggtactac gcagagcctt cccgcctgca agccccgact tctttggcga
    17941 tagaaagcaa cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa aaagctttgt
    18001 cttttgacga agtgatgggc gcgcaagcct gattatcctg aaaatgccgt cttagggcag
    18061 tttttgaaag caccgaagcc gcttttaaag cccgctttgg tgcttttaca tcatgaaagg
    18121 agacgaaatg tcttttgata actatcttgt ccctaccgtt atcgagcaga gcggtcgcgg
    18181 tgagcgtgca ttcgatatct attcccggct tttgaaagag cgcatcgtat tcttggtcgg
    18241 cccggtaacc gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt tggaaagtga
    18301 gaatccggat aaggatattt tcttctatat taactcgccg ggcggttcgg taacggccgg
    18361 tatgtcgatt tacgacacca tgaattttat caagcccgat gtatcgactt tgtgcttggg
    18421 gcaggcagca agtatgggcg cgttcttatt gtcggcaggc gaaaaaggca aacgtttcgc
    18481 cctgcccaac agccggatta tgattcacca gcctttaatc agcggcggct tgggcggtca
    18541 ggcatccgat attgaaattc acgcacgcga actgttgaaa atcaaagaaa aactcaaccg
    18601 cctgatggcg aaacattgcg gccgcgattt ggcagatttg gagcgcgaca ccgaccgtga
    18661 taatttcatg tctgccgaag aagcaaaaga atatggtttg atcgaccaag ttttggaaaa
    18721 ccgcgcttct ttgcagcttt aaaccttgtg aagtagacaa atgccgtctg aaaacagttc
    18781 agacggcatt tgtcttatag ggactgtatt gggatgattt tgttctggtt gcgtttggag
    18841 ccgttttcgc cgtagttggt tttgacatac tcttcaacaa tagccaaaaa ttccgttgca
    18901 atgttgtcgc ctttcagcgt tactttgcgt tcaccatcta cataaacagg cgcgacgggt
    18961 gtttcccccg taccgggcag gctgatgccg atgtcggcca atttgctttc tccgggccca
    19021 ttgacaacgc agcccattac ggcaacgttc agggattcaa ccccaggata aagggtacgc
    19081 catatagaca ttttttggcg caggtaattt tgaacatctt gtgccagctc ttgaaatacg
    19141 gtactggtgg tacgcccgca gccggggcag gcggtaacca tcggcgtaaa cgaacgcaat
    19201 cccatagttt gtaaaatctc ttgcccgacg acgacctcct gagtacgcgg gctgccaggt
    19261 tccggagtca gtgaaatgcg gatggtgtcg ccgattcctt cttgaagcaa gacggataat
    19321 gccgccgttg atgcgacaat gcctttgctg cccataccgg cttcggtcaa acccaaatgc
    19381 agcggatagg cgcaacggct gcccagttcg cggtaaacct gaatcaaatc ctgaaccgcg
    19441 ctgactttgc acgacaggat gattttgtct tcgggcagtc ccaatagaac ggctttttcg
    19501 gcagattcca aagcggagac aatcagtgct tccttcgtca cttcttcggg cggtttcggc
    19561 gcggaagaag cgaggttggc atccatcata cgtttggcga ggctttgatc caaagaaccc
    19621 cagtttacgc cgatgcggac ggctttatcg ttttcagcag cagtccgaat cataaaggca
    19681 aatttttcat cgccttttac gcctttgccg acattgccgg gattgatgcg gtatttggac
    19741 aatgctttgc cgcattctgg aaattccgcc aacaggcgtt cgccgttgaa gtggaaatcg
    19801 ccgataagcg gtgtggcata gcccatatcg tccaagcggc ggcggatttc ggcaactttg
    19861 gacgcggctt cggggctgtt gacggtaata cgcaccattt cggatccggc gtcgctcaat
    19921 tccttaatct gcaatgcggt ggcttttgca tcggcagtgt cggtgttggt catagattgg
    19981 ataacgacgg gtgcttctga accgacggta atatgatcga tgcggacttg atgcgtcttg
    20041 cggcgttgga gtgtgttcat atggtttgaa tctgtttatt gaccgatgag gacggtttgc
    20101 aattcttccg agtaggggaa attcgcctgc aattgtgctt catattcgta tgccgcctgt
    20161 gcgttgccga gggctttggc aattttccag cctagcagca aatcatcggc ctgaaggact
    20221 tctaccctgc tttggtattt tttaaagtag taatcggcat cgcccaactg cccggccagc
    20281 attttggtgc gcgccagttc tttaaatgcg ggtgggaact gcggctgggc ggcgagggaa
    20341 cgtttcaaat aggcttccgc caatccgaat tgcccctgtt ttgcgctgca tatgccttta
    20401 ttcaggttgg caatataagg ggtcgggtag gtggggtcgg ccagggcttt gtcgaaatat
    20461 gccatagatt cggcagggcg gttgagcctg ccgcacagga accagccgta gttgttgttg
    20521 atttcggcac tgtcgggttt gatggagagg gcttgccgga aactttcctg cgccttgtcg
    20581 ttaactttca ggtattgata gatttcggca cggaccagcc aggcaagctc gtttttaggg
    20641 tctgatttca aggcgtcttc aatacttgcc gtcgcctgac ggtagtcctg accgcgcata
    20701 tattccattg ccaactgggt tttgatattg gaaacctgat tggctttttc tgcccgcgag
    20761 gggcggtagg aagtgctgca cgcgcccaag gcaaggacaa gtaataaaga gattcgtttg
    20821 gatggcttaa aaggcataat tacccctgtt gtccgattaa aatctgctgc catttttgtt
    20881 ggcggcgcgt tttatcctga acctgccccg ccaactgtcc gcaggcggca tcgatgtcgt
    20941 cgccgcgcgt ttttcgtacg gtaacgacaa atcctgcctg ctgcaaaata tcgcggaaca
    21001 cacggatgtt ctcattgctg gagcgttcgt atccggagtt tgggaaggga ttgaacggaa
    21061 tcagattgaa cttgcaggga acatctgtga ccagttcgat cagttcgcgc gcatgttgcg
    21121 ccttatcgtt tattccgtcc aacatgacgt attcgaaagt gatgaaatcc ctgggtgctt
    21181 tgaccagata gcgttggcat gcggccatca attctttcaa gggatatttt ttgttcaacg
    21241 gtacgatttg gttgcggact tcgtcattgg aagcgtggag ggaaaccgcc aaagccaccg
    21301 gcatgacatc gcgcaacctg tccatttggg gaaccatacc cgaagtggaa acggttacgc
    21361 ggcggcggct caaaccgtag ccgtggtcgt ccagcatgat gcttaaggcg gtaacgacat
    21421 tgtcgaagtt cgccatcggc tcgcccatac ccatcatcac tacattggaa attacgcgtt
    21481 cgtttttcgg tgtaacaccc atcgctttgt ttgcccacca caattgcccg atgatttcgg
    21541 cagcagtcaa attgcggttg aagccctgcc ggccggtcga acaaaatgta cattccaaag
    21601 cgcagccgac ttgtgaggaa atgcagagcg tgccgcgatc cgattcgggg atgaagacgg
    21661 tttccacccc gttgcccgta ccgacatcca aaagccattt tcgagtgccg tctgaagatt
    21721 tttgagacat catcagcttg ggaatttcga tgcctgcctg ttcgttcagt ttatggcgca
    21781 acgattttgc caaatcggtc atttcgttaa aattttgcgc gccggattgg tgcatccaac
    21841 gcataacctg tttggcacgg aagggttttt cgcccatatc ggcaaaatgt cgggtcagcc
    21901 cttgaaggtc gtagttgagc agattggttt tcatgtgttt gtgtttttct taaatcaagg
    21961 cttcaaataa aaaattgcag ggcaggcaag tttgatttga agccttttgc aacagagtct
    22021 tcagacggca tggatatatt ttatgccgtc tgaagaggag ttgggcggtg tattgtatca
    22081 acgggggcag atttcggttt ggctgaaaaa gtaggcaatt tccaaagcgg cattttccac
    22141 gctgtcggaa ccgtgtacgg cattaatgct gaccgaagtg gcaaagtccg cgcgtatcgt
    22201 gccttcggcg gcttcggaag gattagttgc acccatcagt tcgcggtttt tcaggacggc
    22261 gttttcaccc tctaatacct gaatcataac cggaccgccg gtcataaatt caaccaatcc
    22321 ggcgtagaag gggcggtctt tatgaaccgc ataaaattct tgcgcctctt tgagggtaag
    22381 ctgcttcatt ttggcggcaa cgattttcag accgttctcc tcaaagcggc tgtatatttt
    22441 gccgataacg tttttgccga cggcatcggg tttgatgatg gagatggtac gttcaatcgc
    22501 catgctatat ccttattttt acttaagaag aatcaaatcg gtattctatc aaaaaaataa
    22561 cttatccgcc tgaagccttg ggagataggg tcaggtgcgg ctgttccaaa taaacttcgg
    22621 actgcatttc gaccatccgg ctggcggtgc gggtaaattc ttcggcaaaa tcgccttccg
    22681 tatagatatg gttgacatct accgcgccgg tggcacacag tttgacccgg aaatcgtaga
    22741 gtacgtcaat cagccaagtc agccgccgcg cctccgcctt ttcttgcggt gagagttgtt
    22801 ccaaacctga aataaaaacc atttcataat gttcggccaa atacagatag tcggactgtg
    22861 agcgggggcc gaagcacagt gcgcggaaat caaaccatat ggcacggccg gactcggctt
    22921 tgtggggaat ctcccgacca tggatggtgc tgatgccggg gttcaaatcg gtaatgcctg
    22981 tcatttcttt gaacagtttt gccagttttg cctcattttc ttcattggca ggcgtaaaga
    23041 aaatctcggc ggggcggagg gtacgcagtc ggtagtcttc accgccgtca acgtttaaga
    23101 cggtcaggct ggactcgatg agcgcgattg tgggaagaaa actgctccgg ttttgacctt
    23161 gcgggtagag ttcggaaggc gcgtagtttg aagtcgccac caaaacaacg ccctcgttaa
    23221 gcaggttttc cagcagacgg cctaaaatca ttgcatccgc aatatcgctg acatgaaatt
    23281 cgtcaaaaca caatacgcgg gtttctttgg caatctcggc ggcaacggat ttcaacgggt
    23341 tgctttcgct tttcagggtt ttcagcctct ggtggatttc tgccataaag gcatgaaagt
    23401 ggacgcggcg tttgcggcgg taagggaggc agccgaaaaa agcgtccatc agaaagcttt
    23461 tgccgcgtcc gaccccgcca tagaaataaa gccctttggg gacttgcggg gaacgcaaac
    23521 tcctgcctaa aaaacggttt cttttgcgtt tgaacatcat caattcggtc caaagccgat
    23581 cgaggtgttc gatggcggct gcctgcgcgt cgtcgcggat gaagttgggc agttgtgagg
    23641 cagcctgata ccaagtcagc gggctgtgat tttcaaacgg cggggcttta aaaagttggt
    23701 ctctattact catatttaac cttgttatac ttcttgcggc ggagaaagaa aatgcttacc
    23761 gtcggtatta taaatgattt ccttcaaact tggcgttttc cgtggaaaag aataatgacg
    23821 cgcacaaggg gggggtatga gcaaaggcgg cgtttcaata tggtgcagat atgagctcaa
    23881 atgcctttta tagtagatta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta
    23941 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataatccgt
    24001 agagtcggct tcttccggct cagacggcat acgtttatgc cggcacttct taccgccctc
    24061 gtgccggcta tcccaaaact gctttgaaca ctgaacgctc tttggtttac ttcaaaaacg
    24121 cggcgtgata agcaatatgc tcgccaataa aactggcgat gaagtaatag ctgtgatcgt
    24181 agcctttatg gaaacgcaca tcgaccggct ggtttgccgc acggcaggtt tcgataaaat
    24241 cttcggtacg caattgtgtc ggcaaaaact catcttccaa gccttgatcg atgcgcatac
    24301 cttgcacttt atagccttgt tgaatgagtg agttcgcatc atattgctgc catttttcac
    24361 ggtctttccc taaataagca gtaaaggctt tttctcccca cggcacgagg cttggcgata
    24421 aaataggcga aaaggcagaa acactttgat aacgttcctg attccgcagc gccaatacca
    24481 atgcgccgtg tccgcccatt gaatgcccca taatggaacg tttgccgttg gtaggaaagt
    24541 gtttctcaat cagacggggc agctcgttca aaatgtaatc atacatttga taattcgccg
    24601 cccaaggctg ttcggtcgca ttcaaataaa agcctgcacc ctgtcctaaa tcgtaagcat
    24661 catcgttcgg cacttgctct ccgcgagggc tggtatcgga ggccaccaca attacttgat
    24721 gttctgccgc ataacgctga aagcctgatt tggtaatgaa attttgttcc gtacacgtca
    24781 agccggaaag ccaataaatc acaccaagcg gtcgattttc tggattattt ggcaaataga
    24841 cggcaaattt catttcgcat tgaagcgttt gggcattgtg tgcccaaact tgttgcgaac
    24901 cgccaaaaat ttgatgttgt tcaatcagtt tcatcgcata ccttagtagt gaataacggc
    24961 gcggatcgat ttaccttcgt gcattaactc aaaggcttca ttgatttgat cgagtgtcat
    25021 tgtgtgggtt acaaacggtt ctaactcaat gtcgcctttc attgaatctt ccaccatttt
    25081 cggcagttca gagcggcctt tcacgccacc aaatgccgtg cctttccaca cacggcctgt
    25141 taccaactgg aacggacgag ttgaaatttc ttgtcctaca cctgctacgc cgatgataat
    25201 ggattgtccc caaccacggt gtgcactttc taatgcttga cgcattacgt ttacattgcc
    25261 gatacattca aaggtatggt caatgcccca tttgttgatg tctaacaaca catctttgat
    25321 cggtttatcg taatcgttcg ggttcaaaca gtccgttgca ccgaactgtt ttgccaactc
    25381 gaattttgag ggattggtat caatggcgat aatgcggccg gctttggctt gacgcgcacc
    25441 ttgcacaacc gccaaaccaa tcgctcccaa gccaaacact gccacagagt cgccttcttg
    25501 cacttttgcc gtattatgca ccgcgccaat acctgtagta acgccgcagc cgagcaaaca
    25561 tacttgttca tggttcgctt ccgggttaat ttttgccagt gaaacttctg caacaactga
    25621 gtattcactg aaagtcgaac agcccatata gtgatagatc ggctgacctt gataagaaaa
    25681 acgcgtcgtg ccgtccggca ttaagccttt accttgtgta tcacgcactg agacgcacaa
    25741 gttggtttta cctgaacaac aaaactcaca ttcgccacat tcggcggtgt aaagcggaat
    25801 cacgtgatca ccgggtttta cgcttgacac accttcgccc acagcaacga ccacacccgc
    25861 accttcgtgt ccaagcacca cagggaatac gccttcagga tcgcttcctg ataacgtaaa
    25921 cgcatcagta tggcacacgc cagtgtgggt attgcggatt aacacctcgc ctttacgcgg
    25981 catttctacg tcgatttcca caatttgtaa gggttgattt ggggcgaatg ccaccgccgc
    26041 acgagatttg atggttgaat cggcttgttt catttccatt gtctttcctc ttaattcatt
    26101 gacaaagttg ccttgcctga gcaacatagc aacagtttac actttagagt taactctaaa
    26161 gcaagtgttt tttactgttt ttgtttagaa ggagggaaaa tgacttatac tactgccaaa
    26221 gccgccgaaa aaataggcat ctccgcccac accctacgtt tttacgacaa agaaggtttg
    26281 ttgcccaata tcggacgtga tgaatacggt aaccgctgtt ttaccgataa cgatttgcaa
    26341 tggttgggct tattgcaatg cttgaaaaat acggggatga gcttaaaaga catcaaacgc
    26401 tttgcggaat gtaccgtcat tggcgacgat accattgaag aacgcctttc cttgtttgaa
    26461 aatcaaatag aaaatgtgaa gtgtcaaatt gccgaattaa aacgctattt agatttgctt
    26521 gaatacaaat tggcgtttta ccaaaaagcg aaagcattag gctcggtaaa agctgtaaat
    26581 ttgccgcaaa ttcctgaaac ggcttagttt tagtgcaata agcaggattt ttgtcctgtt
    26641 cgtaatagaa atcaaaaatt tgttgtttaa aaagcggatt gtaagcaatg atatagtgga
    26701 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt gtaaatagta cggaaccgat
    26761 tcacttggtg cttcagcacc ttagagaatt gttctctttg agctaaggcg aggcaacgcc
    26821 gtactggttt ttgttaatcc actataaaaa aatccacgct ctaatccgtt gcttgcttgg
    26881 ctcaaggtat tgaagcaacg gatgaagtta aaccacagaa aaaacgccgc gtaacagcgg
    26941 cgttttgatg ctaaggttta attggcattt tcttttaaag ccaagtcgac ccgctcgcgc
    27001 aattctttac cgggcttgaa gtggggtaca tgtttttcag gtacttccac acgctcgccg
    27061 gttttgggat tgcgaccgat gcgggcagga cgatggttca aatcgaagct gccgaaaccg
    27121 cggatttcga tgcgttgacc tcgggcaagc gatctagtca tggtgtcaac caagactttt
    27181 acgctgtact ctacgtcttt tgccagaaga tgcgtgccgt ttttggcggc aaacacttct
    27241 gccaaacgaa ccattaactc agactttgtc atgtctgcaa ccttattctt gttcgccgga
    27301 gagtttggct ttcagcaggt cgcccaagct ggtggtgccg gcattcgcat tggcggcggc
    27361 attgacggag ttcagtgctt cgcggctttc tttggcatct ttggctttaa cggaaagttt
    27421 gatgctgcgg tttttgcggt caacggtaac gatgacggct tcaacttcgt cgccttcttt
    27481 cagtttggtg gtcaaatctt caacgcggtc ggctgcaaat tcggaagcag gcaggtagcc
    27541 ttctacttcg tcagacaggg cgataacagc acctttggcg tcaacagatt tcacggaacc
    27601 tttaaccaaa gaacctttgt cgttcacgct gatgaagttg ccgaacggat cgccttccag
    27661 ttgtttgata cccaaggaga tgcgttcttt ttccacgtcg attgccaata cgacggcttc
    27721 gacttcttcg ccttttttgt atttgcgtac ggcttcttcg ccggattcgg tccaggacag
    27781 gtcggacagg tgaaccaaac cgtcgatgcc gccaggcaga ccgacgaata cgccgaaatc
    27841 ggtaatggat ttaaccgcgc cggagatttt gtcgcctttg ttgtggttgg cggcaaattc
    27901 ttcccaagga ttggcttggc attgtttcat acccaaagag atacgccggc ggctttcatc
    27961 gatttcgagg atcattactt cgacttcgtc gcccaattgt acaactttgc tcgggtgtac
    28021 atttttgttg gtccaatcca tttcggaaac gtgcaccaag ccttcgatgc cttgttcgat
    28081 ttcaacgaac gcaccgtagt cggtcaggtt ggatacttta ccgaacaggc gggtaccttg
    28141 cgggtaacga cgggtcaggc cgctccaagg atcttcgccc agttgtttca tacccagaga
    28201 aacgcgttgt ttgtcttggt cgaatttcag gactttagct tctacttctt gaccgacttc
    28261 cagaacttcg ctcgggtgtt tcacacggcg ccatgccaag tcggtgatgt gcagcagacc
    28321 atcgatgccg cccaagtcaa cgaatgcacc gtaatcggta atgtttttaa cgatgccttt
    28381 gatgacggag ccttcttgca ggttttccag cagggctttg cgttcttcac ccaaggtagc
    28441 ttccagaacg gcgcggcggg acacaacaac gttgttgcgt tttttgtcca gtttgatcac
    28501 tttgaattca atttctttac cttcgaagtg agaagtgtct tttacaggac gtacgtcgac
    28561 caaagaaccc ggcaggaatg cgcggatgct gctaatcata acggtcaggc cgcctttgac
    28621 tttgccgttg atgatgccgg acaggatgtt gccgttttcc atggcttctt ccagggcaat
    28681 ccaatcggct gcacgtttgg ctttttcgcg ggacagtttg gtttcgccga agccgttttc
    28741 gacggattcg atggtaacgg taacgaagtc gccgacttta acttcaattt cgccttgagc
    28801 gtttttgaat tcagctacat caatcaggga ttctgatttc agacctgcgt ttacggtaac
    28861 gaagttttgg tcgattgcca ctacttcagc ggtaatcacc tcacccgggt tcatttcttg
    28921 cagggtaaag ctttcttcca acagctgagc aaaattttcc atagacatat ataactcttt
    28981 tcggtacacc gccaaggggt gcggggtggg ttggtggata tctgccgtcc ttggcagggc
    29041 agacgggtac ataaggtttc agacggcata tgggggattt ttatgccgtc tgaaacgtaa
    29101 tttgtgcgat tatacctgaa aatttaaact tcacgatacc aatcaagtac tttttttaca
    29161 gtttcttcta tagtcaggcg gcttgtgtcc aaaagcaggg catcgggctg ttgtttcagg
    29221 ggggcaacct tgcggtttcg gtctgcctcg tctctggctt cgatgtcgga caggatgcgc
    29281 tcgaatgcca aaccttcgca ggggatgccg atttgtttgg cgcggcgttc ggcacggatt
    29341 ttggattctg ccgtcaggaa gattttaagt tcggcttggg ggaagacgac cgatccggtg
    29401 tcccgtccgt cggcgaccag tcctttttcg gtcagaaaat cgcgttggcg ttgcagcagg
    29461 gagtcgcgga ctttaggcaa ctgtgcgact gcggatgcgc ccatgccgat ggcttctgtc
    29521 cggatgccgt ctgaaacgtc ttcgccgccg agcaggatgc ggctgtctga aaatacggcg
    29581 ggcagttttt ttgccagttc ggaaacgttt tcttcatcgt gccatcccac gccttgtttt
    29641 tgtgcatata gggcggtcag gcggtagagt gcgccggtat cgagataatc gtatcccaat
    29701 gcggcggcaa cgcgggcggc aaccgtgcct ttgcccgatg cgcccgggcc gtcgatggcg
    29761 atgacttttt gtctgttcat aagggggatt cctgatggtt tggggtatgg gttttgccgt
    29821 ctgaaggatg tgtttcccgt tggggcggat tctacctgtt ttaaaggacg attgtctaag
    29881 cagacggaac gccgcctgcc cggaaatatc cgacagaagc cggcaagccg attcggattc
    29941 cggcgttgcg ggtattgtgg cgggcatcaa tgtaatgccg tctgaagtca gtatggcttt
    30001 cagacggcat taatcggtgt gaatcagaaa aggttgctga cgaaaatcag cagaatcacc
    30061 aacggcacaa gatatttcac ataagcaaac caaatattga ccgtcgtatg gttgccttta
    30121 taaagcaatt cttccttcgc ttcatccttc atcacgaaac cgacaaacaa tgccgagcca
    30181 agcgcggtca gcataaacaa gatgttgccg ctgatgtagt cgaaggcatc gaaaatattt
    30241 ttgccgaaca cggaaacgtc tttccacggg ccatagctca aaatagacgg gatgttgccg
    30301 aaaacgtaga tggcggccaa tacaatcgtg attgcggcgg tacggcggat tttggttttt
    30361 tcctgaatgg tcgtaatcaa tacttcataa atcgtcagcg aagtcgtcaa cgcggcaatc
    30421 aggagcagcg agaagaaaat gacggcgaac acagatcctg cccacatatg cgagaacaca
    30481 atcggcaaac tttggaatac caaagtcggg ccggaatcgg gggcaacgcc gaagctgaag
    30541 agcgacggga aaatcataaa gcccgcaagt atggcgatga tggtattggt aattgccgtg
    30601 ataactgccg tctgaaccag attttcgttt ttatccaaat agctggacaa ggtaatcatc
    30661 acgccgaaac ccaggctcag ggcaaaaaat acctgcccca aaacgaagac gaacagttcg
    30721 gcggtaatct tgctgaaatc aggtttcaga tagaaagcaa ccccttccat tgcgcccgga
    30781 agggtaacgt tgcggacgac catcgcgatt aggaacaaaa acagcagcgg catcagctat
    30841 tttgccgctt tttcaatgcc gccgataacg cctttgacca aaatccattg gttcacggcg
    30901 acaaaaagca gcgtataaaa cgcaatttcc caagggctgt ttcaatgtgt tcggcaaaga
    30961 agccttttgt aaccacaccg tcgatggggc tggaaatatt caaatttcct ccaataatat
    31021 taacgatata gctgattacc cagccgccga gtgccatgta ataagccatg atgccgaacg
    31081 cgccgagcag ccccatccag ccgaccagtt tccaaatttt agcaatgggt ttgccgttca
    31141 tcggaccgcc gaacgcatcc agcgcgttca cgcctttgcg ccgtccgatg acgttttcca
    31201 ccaaaatcat cgggatgccg ataacgagca tggcgataca gaataaaaac acatacgcgc
    31261 cgccgccgtt ttcaccgacc aaatacggga aacgccacgt cgcgccgaag ccgacagtcg
    31321 cgccggcaac agtcaggata taggttaatc ggctggatca ggtttgacga ttggtatttg
    31381 aaggggaaga catattgaaa ccgtgtccga ttgagataaa gcggaaattc tacacgcgtt
    31441 ttttaacagg aattgactga ttgtttttca actgtctagt gattttccat gtaaaaggca
    31501 tatatttcag tgagtatttc tgatgagtat aacccgatga ggaagaatcg gagtttataa
    31561 atagattaat ttgttattct tctacatcgg tgtatggaaa tgaaattttg ttaatcacat
    31621 taatgatagc attttaataa tgatgaaaga gagggaattt gaaataaaaa accatctata
    31681 caaggggaga agtatagatg gcaaaacaca ttacggggaa aacgtcttac tcataagcct
    31741 gcttgaacag gcgttactca gacaaatgga ttatatcgta aattgatttt ttgcgttaaa
    31801 ttgggttaaa ccatatattt aatgagtatg tggatgaata taaatggaat tcttggatgt
    31861 tattttagaa tttcggacaa tcgataaatt tccattcgcc gggtttcagg ttttctgttt
    31921 ccaaatgtgc gaatcgccgg cgatgaaggt gttgcacgcg gttgcctgcg gcggcgacca
    31981 tacgtttgac ttggtggtat tttccctcgg taatggtcag gagcaatgcg gacgggcttt
    32041 ccaatacggc atcggcggcg taaaccgttt cgttttcgtc gtggagcagg acaccgtttt
    32101 tcagggtttc gcaaagcgtt tcgccggcag ggtgcttgag tgtaacttcg tatagcttgg
    32161 gaatttttct gctcggcgaa gtcaggttgt ggttcagttt gccgtcgttg gtaatcagca
    32221 atacgccggt cgtatctgca tccagcctgc cgaccgcctg catatcgatg ttccgcatat
    32281 tgtcggggaa caggctgaat acgctgcggt agtgcttggg tttgtgcgaa gtttcgtaat
    32341 cttcaggctt gttgagcatg atgtagaaat agggttcggg aacgacggtt actgcttccc
    32401 cgtcaatatc caacgtttcg acggatgagg aatcgatgtc tgcatcggtg tcgtccatgc
    32461 aggttccgtt gatgaaaaca taaccgccgg caatcagcca ttggcactgc ttgcggcttc
    32521 ctatgccttg atattgcagg tatttgataa gtttcatgat ggtatgggaa atgtgggatg
    32581 aaaaagacag gactgagaaa gtcctgtcat gtgccgtatc cgaatcagct tccaatcagt
    32641 ttgaccctct gtcccggatt gatggtgttc aggttgggat tgagccggcg gatgtcgtcg
    32701 atatggatat tgaagcgcgc ggcgatactt ttgaaggtgt cgcctttgcg cgcggtgtag
    32761 gatacttttt caatacgggt ttgggcaggg gctgcctgcg ccaggcggag tacctgtcct
    32821 ttttggatgg tgttgccttt gatgttgttg gcgacaatca ggtcggctac actgacgttg
    32881 tagcgtttgg caatgttgaa cagggtgtcg ccttctacaa cgcggtggat gctggcatgg
    32941 agcggggaga tttcggcttc gccgtcggca gctttggcta cgcgcgtttc caaacgtacc
    33001 cggcgtttgc cgtcggcggc aacggtatgt tgcggagatg aggaaatgtt gtgttcggca
    33061 actgtgtcag gcgtgccgat gacggcagag atggtttctt cagcctgtcg gcgcaggttg
    33121 tttcgggcaa caagctgcat gagttcgtct gccgcctgca tgtcgttttg ggggacgatc
    33181 tgcgcgagtg ttgcggtttg ggtttcagac ggcatggcgg tctgtttttc ggtttgaggt
    33241 tgcgctgcgg ctgtcgcaag ggcaggttcg gcaatacgga caaggggatc gggttccgta
    33301 cggacggttt tctgcggcaa aggtgcgacg gtaatgtccg ctgtctgtgc ggcggcgggt
    33361 cggattcggg caatgccgac gttcaccgtg cctgccggca tattggaacg gtaggtgtcg
    33421 ggtgtattgt cgatgtcgat ggaaacgacg gattccgatg ccgtctgaag ggttttgccg
    33481 ttcttggcga caaggatgct gcgtcctgcg ttgacaaggt tgccgttcag gttgttgagg
    33541 cgtttgatgt cggcaatgct catgccggtt gccgtcgaga tgtcggacag gctggttttg
    33601 gcggcaggcg tatagacttc ccatgaaaac aggctgtcgg gtgcggcgtt gaggtagttg
    33661 ctttggaagg tttgtacgga cgcgacagga agcagcagtt tgcgtttgct tttggggata
    33721 aacgcgggga cgttgaatgc ggggtttagg gcgagcagct cgctttgcgt gatgccggca
    33781 agccgggcga tggcttcgtt gtcgagcgga cgatccggtt cgactgcctg aaaatagggt
    33841 ttgttgtcta tgtcgctgat attcatgccg aaagattggg gagtggcaat aatgttgcgc
    33901 acggcgagca gcttggggac atagttgcgc gtttcgttgg gcatacgcag gttttcgtag
    33961 gtcggttcga gcccttgggc gcgggcgcgg ttgatggcgc gtccgacgtt gccttcaccc
    34021 cagttgtagg cggcaaaggc aagcggccag tcgccgaaca gtccatagag gtattgcaga
    34081 tagttgagtg cggcatcggt ggcggcgtaa acgtcgtgcc tgccgtcgta aaccggtgtt
    34141 ttttccaggc cgtaatgcct gccggtagcg ggcatgaact gccacaggcc cgatgcgccg
    34201 acgtgtgatt tggctttggt gacgaacgcg ctttcgatga agggaagcag ggcggcttcg
    34261 gcgggcatat tgcgtttttt gacttcgttg gcgatatggt acatataggg tctactccgg
    34321 ttgatgaccc tgttgaaata gctgtggctt gcgatgaatt tgctttcgtg gcggcgtacc
    34381 agttcgggat tgacttcgcc catccggaag ccttggcgca gctcgctcca caggctgccg
    34441 gattggaaat attgttttgt cgggggcagg tcgagtattg aagagtttaa gcgcataatc
    34501 gccaaaccga tttggtgtga tgaggtgttt tgggcgtata ggaaacccgg acaaacggac
    34561 agacctgacg cggtcagggc gatggttttg agtttggaca tgatttatat cggcggaata
    34621 aacatatttt atcgtccgat ggtactgttt gactgaaaaa gcgtcaagtt ttacaggaac
    34681 tttatgcgga ttcggaaatg ggcggccgat gtgttttttg acagaaatcc tgtttggaat
    34741 agaatatcgg caggcaatct gtttgaaacg gacgcaatat ggatgcatgg tttgaagata
    34801 cggcgatggg gcggtatgtt gcaaaattgg aacaggattt cttcgggcgg tatctagatt
    34861 cataccgttt ttcgggaatg tgtgcggttc aggtgggcgg tccgtggctg agcctgtctg
    34921 aagatgttgt ctgtgtgccg cgagatatgt cgatgtcggc ggagaatatg gctttggcgg
    34981 atgtttctgc ggatatgctg ctttttccgc atacgcttga aggcggtatc ccttcgcaaa
    35041 tcctgtcgga agcgcaccgg atattgaaac cgtgcggacg cttgatgctg acgggtttca
    35101 atccgtattc gctctgggga ttcagccgtt ggtttgacgg cgaacgcctg ccggaaaaac
    35161 ggttttgtct gccgctgccc gagttgaaaa ggcagcttgc ggatgtcggt ttcgatattg
    35221 aatttgggaa atttatggtg tatctgccgc ccgtttcgtc gatcgggcaa atacgctttt
    35281 ggcggtttat ggaaaaggcg ggcgaccgtt ggtggccgca atgtgccgca gtgtacgctt
    35341 tggttttggt caaaagggcg gcgggcgtaa cgcccctgcc cgcgtgggac gggtatttgg
    35401 gcggcaaagc ccttgcggcg ggtgcggcaa gggttgcgga ttagggacag ccgtctttca
    35461 gacggcatct gtcagaaaaa atgtcgggag gggttatgcc ggaattgccg gaagtggaaa
    35521 cgacgttgcg cggcatcgcg ccgcatattg aagggaaaac ggtggaagcc gtggtattgc
    35581 gccaattgaa gctgcgctgg cagatcaatc ccgatttggg ggagattttg tccggtcggc
    35641 aggttttgtc ctgcggcagg cgtgccaaat atctgattgt ccgtttccaa acggggattt
    35701 tgctgattca cttggggatg tcgggcagct tgcggatttt tacgccgtcg gacggacgta
    35761 tcggcaggcc ggacagacac gatcacgtcg atattgtgtt ttcagacggc acggtcatgc
    35821 gttaccgcga tccgagaaag ttcggcgcga tactttggta tgagggaatc gaagaacatc
    35881 atccgctgtt ggaaaaactg gggccggagc ctttgtcgga ggcattttgt gcggattatc
    35941 tgtatgcaag gctgaaggcg cagaagcgcg cggtcaaact tgccctgatg gacaatgcgg
    36001 tcgtggtcgg tgtgggcaac atttatgcca acgagagcct gtttagggcg ggcatttcgc
    36061 cccaccgtcc tgccaaccgc ctgaaaaaga aagagtgcgc gcttttggtt gaaaccgtca
    36121 aagcggtgtt gcagcgcgcc attgaaacgg gcggcagtac gctgagggac tttgtggaca
    36181 gcgacggcaa aagcggctac ttccaacagg aatatacggt gtacgggcgg cacaatcagc
    36241 cgtgccccca gtgcggcggt ttggttgtga aagaaacttt ggggcagcgc ggcacgtttt
    36301 actgcccgaa ttgtcagaaa tagggcggaa aacggtttca gacggcattt tatcggtatg
    36361 ccgtccgaac gtttcaacaa caaacaccga ttatcgggaa agaattgctc atgtcttcaa
    36421 ataaagcttc attttttaca cgtctgcgcc gcttgtgccg cttgacggtc tggctgttca
    36481 aaaccgggaa aaacctgcgc ggtattgacg gcggttgccc cgagtcgcgc aatcgggcgg
    36541 taatcgagtt gggcaggggg gttttggcgg ctttggatat cggattggag gtgggcagac
    36601 ccgcacccga acatccgaac ggtgtcttgg ttgccgccaa ccacgtgtcc tggctggata
    36661 ttttcgcgat gagcgcggtt tatccgagca gctttatcgc caagcaggaa atcaaaagct
    36721 ggccggtatt gggcaagatg gggcagaacg cgggaacggt gttcatcaac cgcaattcgc
    36781 ggcgcgacat cgaaccgatt aaccgcgccg tctgcgaaac cttgcaacgc ggtcaaaacg
    36841 tcagtttttt ccccgaagcg cggacttcct ccggattggg gcttttgccg ttcaaagccg
    36901 cgctgttcca atccgccatc gatgcggggg caaaggtttt ggcggtcgcg ctgcgttatt
    36961 atgacgaaac gggaaaaagg acggctcgtc cctcatatgc cgatgtcggt ttgccgacct
    37021 gcctgtggcg catcgtgtct atgaaaaaat tgacgataaa agtcgatttc gtttgcgtgg
    37081 cggatgcggc ggaaagcgaa gaccgttatg ctttaaaaga taaaatcgaa gaaagcatcc
    37141 gtgccgttgt cgccgacgat gcggatatcg ctgtctgaaa ccggttgtcg gaatgtggca
    37201 gtatgattcg cttttgtgga tgtatagtgg attaaattta aaccagtaca gcgttgcctc
    37261 gccttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggtttcgt
    37321 actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt
    37381 aaggctgcgg caacgggagg acctcaaacg aaatggcgta aggatagttt gttgtattca
    37441 taaaatatgc cgtctgaacc tgtgttcaga cggcatattt tatatgggat taacggcgga
    37501 acctgctgcc gtcgaaatca tcgaaagcct cataagccgc ccggcattgg gttttctgtt
    37561 ccttataagg cagggagcgg aatgctttgc gcgccttttt gttttcgcgc aattcctgca
    37621 cgttgccttg cgatcggtac cacgcggcac ggcgttccaa atatttcctg cattcggggt
    37681 gcagcatctg cttgtttgcc ttgcggatat gtttggtatc gcggtgggcg ggttctgacg
    37741 taccggcagc ggcagttgtc gataccagtc ccaatattgc agacaacagt atggcaagca
    37801 tctttttgac agatatgacg atcctttcag tttgtcattc cttccagtat aggatggaag
    37861 gatggaacgg gcaaattgtc ggcagtttca gactcattca gccgacaaaa cttgccgttt
    37921 gatatgcgga ttcaaataac acattaatct caataataaa ttccaatcgg taagaaaatg
    37981 gaatttgtcg gcggcggcgc ggcaaaatca tactttgcaa aatttaacaa tttgcagggg
    38041 cagaaaacag gaagctttcc tttttcgtcg gaaaatcctt atttcgccgc cttgtagccg
    38101 gagccggtca aaaggcaaaa aatttacccg ttttttatcg gtaaagaatt atcagataaa
    38161 acaaatatta taggaaaaat acgacaggcg ggttttatcg cgcattgcct gaaactgaaa
    38221 aatacaaccg ttgtcaagac tggagaaaat gccaaaaatc cactatattg tctgccttaa
    38281 tttatttgaa aagactgtgt cttgaatatc aagagtggaa gaggaagcga tgaatacacc
    38341 gactgatttg aaagtaacca aacgagacgg aagattagaa gccattgatt tggataagat
    38401 tcaccgtgtc gtcacttggg cggcggacgg attggaaaat gtttccgtat ctcaggtcga
    38461 gttgaaatcg cacatccagt tctacaacgg catccgcacc gacgacatcc acgaaaccat
    38521 catcaaagcc gctgccgatt taatttcgga agataccccg gactaccaat accttgccgc
    38581 gcgtttggcg attttccatc tgcgtaaaat agcctacggc gagtacgagc cgccgcacct
    38641 ttacgaccac gttaaaaaac ttaccgatgc cgcaaaatac gacaggcata tccttgagga
    38701 ttacagccgc gaagaatttg acgagctgaa cgcctatatc gaccacgaac gcgatatgtc
    38761 cttttcctat gccgctgtca aacagctcga aggcaaatat ctggtacaga accgcgttac
    38821 ccgccagatt tatgaaacac cgcagttctt atatgttctg gtggcgatgt gtcttttcag
    38881 caaatatccc aaagaaacac gcctcgatta cgtcaaacgg ttttacgatg ccgtttccac
    38941 atttaaagta tcgctgccga ccccgattat gagcggcgtg cgtacgccta cgcgccagtt
    39001 ctcaagctgc gtattgatcg aatgcgacga cagcctggat tccatcaacg ccaccaccag
    39061 cgcgattgtg aaatacgttt cccagcgtgc gggcatcggc atcaatgccg gacgtatccg
    39121 cggactggac agcgaaatcc ggggcggcga agcacggcat accggctgca ttcccttctt
    39181 taaaatgttt caggcggcag tcaaatcctg ttcgcaaggt ggcgtgcgcg gcggcgcggc
    39241 aaccttgttc taccccttgt ggcatatcga agccgaaagc ctgctggtgt tgaaaaacaa
    39301 ccggggcgtg gaagacaacc gtatccgtca gcttgattac ggcgtgcaaa tcaaccgcct
    39361 gctgtacacc cgcctgatta agggcggcaa cattacgctg ttttcgccca acgaggttcc
    39421 gggattgtac gaagcgtttt ttgccgacca agacgaattt gagcggctct atacgaaata
    39481 cgagcaagac cctgatatcc gcaagcgcat cattccggct gccgacctgt tttccacgct
    39541 gatgcaggag cgtgccggaa ccgggcgcat ctacattcaa aacgtcgatc actgcaatac
    39601 gcacagcccg ttcgatccgc gcgttgcgcc tgttcatcag tccaacttgt gtatggaaat
    39661 cgcgctgccg accaaacctt tggacaatat caacgacccg aacggcgaaa tcgccctgtg
    39721 taccctgtct gccttcaact tgggggcatt aaacagcttg gacgaattgg aagggcttgc
    39781 cgatttgacc gtgcgcgcgc tcgatgcact tttagattat cagggatatc cggtagaagc
    39841 cgcgcgtacc tccactatgg gccgccgttc gctcggtatc ggcgtgatca actatgcgta
    39901 ttatttggcg aaaaacggtg tccgctacag cgacggctcc gcgctcggtc tgacccaccg
    39961 tacctttgaa gccatacagt attacctgct caaagcatcg gcaaaccttg ccaaagaata
    40021 cggtgcgtgc acgctcttta accaaaccgt ttattcgcaa ggcaaactgc ccatcgacac
    40081 ctacaaaaaa gacttggacg ctgtgtgtaa cgagcctttg cactacgatt gggaaagcct
    40141 gcgtgccgac atcgtcaaat acggcctgcg taactctact ctaaccgcac tcatgccgtc
    40201 tgaaaccagt tcgcaaatcg ccaacgccac caacggtatc gagccgccgc gcggattggt
    40261 aacggtcaaa gcatcgaaag acggcatttt gaaacaagtc gtgccggagt ttgaaaccct
    40321 gaaaaatgcc tatgaaaccc tgtggcagct tcccggcaac gaaggctatc tgaaacttgt
    40381 tggcgtgatg caaaaattcg tcgatcaggc gatttccgcc aacaccgcct acgacccggg
    40441 caaattcgaa ggcggcaaag tttctatgaa acaaatgctc aaagacctgc tgaccgccta
    40501 caaatacggc gtcaaaaccc tgtactacca taacacccgc gacggcgcgg acgatacgca
    40561 gaccgatatt caggatgacg gctgcgcggg cggggcttgt aagatttgat gaaaggggga
    40621 gttttcagat ggcctttaga ttaataaatc atctgaaata taaaatatga aaaataaaat
    40681 acaaaatcaa ttagataata tagttacctt aaataatgag aaatttactt ttattgattt
    40741 atttgcggga ataggtggtt ttcgcattgc aatggagaat gttggtgggc gatgtgtatt
    40801 ttctagtgaa tgggatgata aagcccgtca aacctaccaa gtaaatttta atgatattcc
    40861 ttatggagat attacattaa aggaaaccaa agcagctatt ccaagtaagt ttgatgtatt
    40921 aacagcagga ttcccgtgtc agccattctc tatagctggt gtttcaaaga aaaaaagcct
    40981 aggacgagaa acaggcttct tagataaggc gcagggaact ctattttttg atgttgctga
    41041 aattattgga aaacatcgac ctaaaatttt tcttttagag aatgtgaaaa accttgtttc
    41101 gcatgacaaa ggaaatacat ttaaagtaat taaagggact ttagaagagc ttgactatca
    41161 gatattttat caagttatga atgcaaaata ttatgttcct caaaataggg agcgtatttt
    41221 tattgtaggt tttgatagac aatattttaa taaggaaata aatttcaatt ttccttcccc
    41281 accagaatca caaccaaaat taaagcaaat tttggaagat gatgtagata attcttttac
    41341 tctttctgat aacttatggc tttaccttca aaattacgct aaaaaacata aggcaaaggg
    41401 taatggattt ggttttggat tagttgattt agatggaata tcacgaactc tatctgcacg
    41461 atattacaaa gatggttccg aaatactcat ccctcagaaa ggaaaaaatc ctaggaagct
    41521 gacacctagg gagtgctcgc gtttaatggg atttcctaaa gattttgtta ttgatgcagt
    41581 atccaagaca gctgcataca agcagtttgg caattcaatt gctgtaccgt tggttcaagc
    41641 tattgctaaa caaattataa atgagttaaa aaatgaatga cttggcagct cagacaatac
    41701 aagtagtaaa taaatccgat gacttagtag cttcagcaat acagacagca aataaatcaa
    41761 ttgcagtata ctgtcgttat attcgtccca atgatgttgg tactactggt agtcaccaat
    41821 caggatttta tattcaaaaa catttttcag acgacctctt tgatgtagtt tgccaaaagg
    41881 gaacaaataa aactatttca atcaaaatta attggcaaga tggaagcgtc actaatagca
    41941 attttaaata ttacggccaa ggcacgagaa atgaggcgag aattacgggt tttggcaaga
    42001 attttgaatt tttaagtgat aaatatagtg gttccttatt ggtattgtgc agagcttgtt
    42061 ataaagattt attattccat gcttttgttt tatcttcaga tgaagatatt gaaatattta
    42121 tcgcagaaac taacatttta ccaggaagtt tatatttacc taaaaaacaa gaagtagagg
    42181 ataacctaac aaaattattt tcactatttc ctaacttccc caaaactgaa gaaatggcag
    42241 ttttggcaag ggaaaatatt gcattaaata aaactaacgt tagtaatgtg ttaaaacaat
    42301 gggtttcaaa ggaatatgaa ttattttcca tttttgaaaa acgtgaattt gaaattatta
    42361 aatcaaaaat tactgatttg gatagcttca ttaattttgc acattctttt acaaatcgca
    42421 gaaaagcaag agcaggaaaa tctttagagt tacatttatc aagaatattt gacgaatttt
    42481 ccttaaaatt tgagacgcaa gcgaagacag aagggaaaaa gaaaccagat tttttatttc
    42541 ctggaagtga agagtaccat gcgatggatg aatcagggaa tttcatattt caaactgaaa
    42601 agctgacaat gctaggttca aagactactt gtaaggatcg ctggcgccaa gttttaaatg
    42661 aagcagatcg aattcctcat aagcatttat ttactctaca agaaggtata tctgatactc
    42721 aaattcagga aatgagtgat gagaatttaa ccttagttgt ccctaaagaa tccgtaaaaa
    42781 catttggaac ttttgggaaa acccatgttt taactttaga aaattttatt aaatatataa
    42841 agtctcagca ggttagttaa aacttacaga tcatttttaa ttaatataga gaatagatta
    42901 tgtcctgcga acacttaact atgtcataca gcacctttcc caaaaccaaa aacgacgcgc
    42961 tgaatgagcc gatgtttttc ggtcagccgg ttaatgtcgc ccgttatgac cagcagaaat
    43021 acgaggtatt tgaaaaactg attgaaaaac aattgtcgtt tttttggcgg ccggaagaaa
    43081 tcgacgtgtc gcgcgaccgt atcgactacg ccaacctgcc agaacacgaa aaacatattt
    43141 tcatcagcaa tctgaaatac caaacactgc tcgattccat ccaagggcgc agtccgaatg
    43201 ttgccttgct gcctttggtt tcgattcccg agttggaaac gtggattgaa acgtggagct
    43261 tcagcgaaac catacactcg cgcagctata cccacatcat ccgcaatatt gtgaatgatc
    43321 cgtcggtcgt gtttgatgat attgtcgaaa acgaatacat taccgcccgc gccgaagaca
    43381 ttgcctgcta ttacgacgac ttaatcgaat acacccagta ttacaatctg ttgggcgaag
    43441 gggtgcacaa tgtcggcgga aaacccatta ctgtgtcttt gcgcgggttg aagaaaaaac
    43501 tctatctctg cctgatgtgc gtcaacgtgt tggaagccat ccgtttctac gtttcattcg
    43561 cctgctcgtt tgcttttgcc gagcgcgagt tgatggaagg caacgccaaa atcatcaaac
    43621 tgattgcccg cgacgaagcc ctgcacctga ccggcacgca gcatatgctt aatctgatgc
    43681 gttcgggtgt cgatgattct gaaatggcag aaattgccgc tgagttgcag gacgaatgtt
    43741 tccaactctt caaaaaagcg gcggaacagg aaaaagaatg ggcggcatat ttgtttaaag
    43801 acggttcgat gattggtttg aacaaagaaa tcttatccca gtacgtcgaa tatattacca
    43861 atctgcgtat gcaggcggtg gggttgccgg ccggatttga aggcgcaaat caaaacccga
    43921 ttccgtggat taatgcgtgg ctgtcgtccg ataacgtaca ggtcgcgccg caggaagtgg
    43981 aaatatcctc ttatttgatc ggtcagatcg attctgaagt gaatacggat gatttgggcg
    44041 attttgagtt gtaaacgttt tttgaaaaaa tgccgtctga agccggatgt ttcagacggc
    44101 atttgtatgt tgtatgatgg gaaaaacagc gggtaggatc ggttattgtt ccttttatag
    44161 tggattatag tggattaaca aaaatccgga caaggcgagg caacgccgta ctggtttaaa
    44221 tttaatccac tatatttttg attggtcgga aatggcgcgc attggtacga ataagggttt
    44281 ttttgagctc ttggagggcg agaccctgtt ggaaggcttg gaacggacgg ggcatatggt
    44341 ggaatatcag tgccgaagcg gatattgcgg atcgtgccga gtgaagattt tggagggaag
    44401 cgttacttat cgggagccgc ctttggcttt tttggggcgg gacgagatac tgccgtgctg
    44461 ctgttgcgtc gagggggatg tcaggctgga ttgcggtttg tcgggggagg atgaggggct
    44521 gtctgacagt ttgttgaaat aaaagccgtc cgaacattgg ttcggacggc tttttgacgg
    44581 tattaccggt tatgcctgat gagtcggtaa aaggtttcac cgtcttggat ataacccagt
    44641 tctacccggg cgatttccga aatggcttct tgaccgtttt ccaaatcata gacttcggcg
    44701 gcaagggaat gattgcgtag ggcgagtgtc tggttttttt cttcttgaac ggcaatctgt
    44761 tctctcagac tgctgttgcg tccgatgctg cctttgccga accagaggct gtattggcaa
    44821 cagacaagtg cgaaggataa aacgacagtt acccacttca taccttaacc ttttttattt
    44881 gcccagttgg tagaatgcgg ctttgctggg gtagtcggcg gcttccgcca attcttcctc
    44941 gatacgcagc agttggttgt atttcgccat gcggtcggaa cggctcaaag aaccggtttt
    45001 gatctgcata cagttggtgg cgactgccaa gtcggcaatg gtgctgtctt cggtttcgcc
    45061 ggagcggtgg ctcattacgc tggcgtagcg gttgcgtttg gctaagtcga cggctttcag
    45121 ggtttcactc aaagtaccga tttggttgac tttgaccaat agtgcgtttg ccacgccttt
    45181 ttcaatgcct tcggcaagga ttttcgggtt ggtaacgaag aggtcgtcgc caacgagttg
    45241 gactttgccg cccagttttt cggtcagcag tttccagcct tcccagtcgt tttcatccat
    45301 cccgtcttcg atggagatga tggggaactc gttgaccagg ccttccagat attcggcaaa
    45361 ttccgcgttg gtgtaggagc ggccttcggc ttccaagtgg tatttgccgt ctttgtagaa
    45421 ctcgctggac gcgcagtcca atgcgaataa tacgtcttcg cccgctttgt agccggcggc
    45481 ttcggtcgcc tcgaccatca gttgcagggc ttctttgtgg ctgttcaggt tgggggcgaa
    45541 accgccttcg tcgccgactg tggtcgggaa gcctttgctg tcgcacagtt ttttcaaggc
    45601 gtggaaaatt tccgcaccgc agcgcaacgc ttcgcggaaa gattttgcgc cgacgggcat
    45661 aatcataaac tcttggatgt tcaggctgtt gttggcgtgt tcgccgccgt tgatgacgtt
    45721 catcatcggt acgggcaggg acatcgggcc tgcgccgccc aagtagcggt aaagcggcag
    45781 gcctgagtct tcggcagcgg cgcgtgcaac cgccatagaa accgccaaag tcgcattcgc
    45841 acccaaattg cctttgtttt cagtaccgtc caattcgatc atgatttggt cgatataaga
    45901 ttgctcgttg gcatcgatac caatgagggc ttgggcgatt tggttgttga cgtgttcgac
    45961 cgccttcaat acgcccttgc ccgaataacg ggatttgtcg ccgtcgcgaa gttccaaagc
    46021 ctctttttga ccggtggacg cgccgctcgg tacggctgcg cgtcccatta cgccggattc
    46081 gagcaataca tcacactcga ctgtggggtt gccgcgtgag tccaaaattt cgcgggcgaa
    46141 aatatcaacg attgcgctca tgaatgttct ccaagtgaag tgaagggagg taaaaaatgg
    46201 gagggcttca gacggcatct ttgccggaaa gccgccaact taaaactgct gaaagtaggg
    46261 cggttgccat aaaaacaata ctggaaatgc cgtgccacat tccgaaggat ccgcctacaa
    46321 acgacaacag ccaattttca tgtccgtatt tcagtgcctc gataacgggt gtaatcaaga
    46381 atcggttggc ggcaagggcg gacaataaaa aaagcagcag ggcggtttgc cgccgggtta
    46441 gggcggcaaa ggcggcagcc agtaccgcgc cccaaacggc aagcccgctc caagagagga
    46501 tgtcgaacaa tacgccggca atttcgcccg cctgcatttt gggcagcatt ttgaacagca
    46561 ccggcgcgac gatataaccc gccataatct gcataccgag ccacaaggtt gcggcatacc
    46621 ggctgatttt tcggaaagtc tgcatcataa ggcgaaaccg tgttactcga ttgttaaaat
    46681 cggttgtgat ttgattaaat cgtccaatgc tttgatgcgg attaaaaaat cttctaaaag
    46741 gtgcagcggc agcgcgctgg ggccgtcgca ttttgccagt ttcggatcgg ggtgcgattc
    46801 gaggaacaga ccggcaaggc gggttgccat gcctgcaagt gccaaatcca aagcctgtgc
    46861 gcgacgaccg ccggatgcgg cagaaccagc atcgcgggtt tgcagggaat gggtaacgtc
    46921 gaaaataacc ggcaggttgc cgcaggtttg tttcatcacg cagaaaccga gcatatcgac
    46981 aacgaggttg tcgtagccga agctgctgcc gcgttcgcat aaaatcagtt tcccgttgcc
    47041 ggcttcgtgg aatttttcca caatgttttt catttgagag gggctgagga actgaggttt
    47101 tttgatgttg acgacgttgc cagtttttgc catggcaacc actaaatcgg tctgccgcgc
    47161 aagaaaggcg ggaagctgga tgacatcgca cacttcggcg acgggttggc actgatgggg
    47221 ttcgtgtacg tcggtaatga cggggatgcc gaactctgct ttgacttttt caaaaatctt
    47281 taagccttct tccaagccta cgccgcgata agaatggatg gaggaacggt ttgccttgtc
    47341 gaaagaggct ttaaagatat agggaattcc gagtttgcgg gttacttcga cgtaatgcgc
    47401 gcaggtttgg agggtggaat ccaagctttc caaaacgttg atgccgccga ataggacgaa
    47461 gggcgagttg ttgccgatgg tgatgtcgtt gattttaata tccataatcg atgtccgttt
    47521 gcggaattac agcacggtgt gcggcggctc gtaggagatg atgtcgcgga ttttgttgtt
    47581 cccgtccact aagacgactt tgggttcatg cgcggcgatt tcgggttcgg agagttggac
    47641 gtaagacatg atgatgacga tatcgccttt ctgcaccagc cttgccgcag caccgttcag
    47701 acaaatcacg ccgctgccgc gtttacctga aatggtatag gtttcaaaac gttcgccgtt
    47761 gttgttgttg acgatggcga ctttttcgtt ggggtagatg cctgccgcgt ctaacagatc
    47821 ttgatcgacg gtaatgctgc cgacgtagtt caaatcggct tcggttacgg tggcgcggtg
    47881 gatttttccg ccaagcatag tacggaacat gtatttcctt ttggtttgtg tgcggtctga
    47941 cgggcgcgat gccgtctgga aatctgaatg ccgtattata gtggagtttc cttcaaattc
    48001 ataatggcgg ctttcggaca aggaaaacgg tgtttcagac ggcattgcgg tgtcggcttt
    48061 atttgggcag gcatctgttt tgcaaggttt ttaaaacgtt ttttacggtg ttgattctga
    48121 cctcgatatt ttccatctct gcggtaaacc gcagcttatc ggcgccggca aggcggtatt
    48181 ttttgtcgtt ctgaatcagc aggatgattt cggttggatc gacattgtgg tttttgccga
    48241 aggttaccgt taccgattcg ccggccgcat cgatggcatc aatacccaat tcttttgccg
    48301 caagccgtaa gtggtggctt tcgatgaggg ttttgacggg ttgttcgggc aggccgaagc
    48361 ggtcgacgag ttcttcgtgt atggcgttga tttgctgcac ggtttcgcag acggcgaggc
    48421 gtttgtagag gacgagacgc tcgtggatgt cggggcagta gctttcgggc agcagagcgg
    48481 ggctgtgcag tttgatttcg gtggtaatgc ccaatggcgc gtcgaggtcg ggctggcggc
    48541 cttttttgag gtcgcgcacg gcttgtttga gcatttcggt gtagagcgtg aagccgacct
    48601 gtatcatttc gccggactgc ccttcgccga ggatttcgcc tgcgccgcgg atttccaaat
    48661 cctgcatggc gagggtgaaa cctgcgccga gttcgtctgc tgccgcgatg gcgtcgaggc
    48721 gtttttctgc gtctttggtg atgtattcgg gcgtgagcag gtaggcgtag gcttggtgat
    48781 ggctgcggcc gacgcgcccg cgaagttggt gcagttgcgc cagcccgaat ttgtcggcgc
    48841 ggttgatgat gatggtgttg gcgttgggga tgtcgatgcc ggtttcgatg atggtggaac
    48901 agagcaacac gttgaatcgt tgctgtaaaa agtcgcgcat gacttgttcc aactcgcgct
    48961 cgcgcagttg tccgtgcgct acgccgatgc gggcttcggg cagcagggtt tccagccgct
    49021 cgcgcatatt ttcaatcgta tctacttcat tgtgcaggaa aaatacttgc ccgccgcgtt
    49081 tgagttcgcg caacacggct tcgcgcacgc tgccttcgct gaagggtttg acaaaggttt
    49141 tgacggcgag gcggcggctc ggtgcagtgg taatcagcga gaagtcgcgc agaccttcca
    49201 acgccatact taaagtacgc ggaatcggcg tggcggtcat ggtgaggata tcgacattgg
    49261 cgcgcaggcg tttgagctgc tctttctgtc gcacgccgaa gcggtgttct tcgtcgataa
    49321 tcactaaacc taagtttttg aattttatgt cgtcctgcac caatttgtgc gtgccgataa
    49381 tgatatcgac cgtgccgtcc gccatgcctt cgagcgcggc tttggtggct ttgctgttgt
    49441 tgaaacgcga aaggccggcg actttcacgg gaaaatcggc gaaacggtcg gcaaagtttt
    49501 gcgcgtgctg ctcgaccaga agcgtggttg gggcgagtac ggcgacctgt ttgccaccca
    49561 tcaccgccac aaacgcggcg cgcagggcga cttcggtttt gccgaaaccg acatcgccgc
    49621 acacaaggcg gtccatcggc ttcgcctgcg tcaagtcttt aatcacggcg gcaatggcgg
    49681 ctgcctggtc ttcggtttcc tcgtagccga agccgtcggc aaacgcctga taatccaact
    49741 cgttgatttc aaacttgtgt cccgattggg cggcgcgttg ggcatagaga ttgagcaact
    49801 cggcggcggt atcgcgcgct ttttcggcag ccttgcgttt tgccttgttc cacgcgccgc
    49861 tgccgagctt gtgcagggca atgttttcat gcgcctgacc ggagtagcgg ctgattaaat
    49921 gcagttgcga aacagggaca taaagctgcg cttcgcctgc gtattcgagc aacatcattt
    49981 cgttggtttc gccgcccaag tccatcgtta ccaagcccat ataccgcccg atgccgtgtt
    50041 cttcgtgcac gacgggatcg ccgatgttga tttcggcaag gtcgcgcaac aggccatctg
    50101 aaacggcggc gtgtttcttg cgatgatatt tgcgcgagcg ggcaacatat tgatacaaat
    50161 cggattcggt aatgacagcg atggctcttt gagcggcaac tgctttgccc tctccccaac
    50221 cctcccccac gggggagggg gcaggttgct gttggatttg cccgtgcaga ttacttttac
    50281 cggggggcag gctgctttcg gttgaaacgg
//
LOCUS       DAWFIT010000003        46661 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFIT010000003 DAWFIT010000000
VERSION     DAWFIT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.003"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..485)
                     /locus_tag="WGQ85_000153"
     CDS             complement(<1..485)
                     /locus_tag="WGQ85_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HEZ0749670.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYEMYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKPFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDALYGRVHFGGQNN
                     T"
     gene            588..803
                     /locus_tag="WGQ85_000154"
                     /pseudo
     CDS             588..803
                     /locus_tag="WGQ85_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(955..2013)
                     /gene="trpD"
                     /locus_tag="WGQ85_000155"
     CDS             complement(955..2013)
                     /gene="trpD"
                     /locus_tag="WGQ85_000155"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0749671.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(2077..2667)
                     /locus_tag="WGQ85_000156"
     CDS             complement(2077..2667)
                     /locus_tag="WGQ85_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0749672.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3087..5363
                     /locus_tag="WGQ85_000157"
     CDS             3087..5363
                     /locus_tag="WGQ85_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0749673.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            6047..6154
                     /locus_tag="WGQ85_000158"
                     /pseudo
     CDS             6047..6154
                     /locus_tag="WGQ85_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6299..7096)
                     /locus_tag="WGQ85_000159"
     CDS             complement(6299..7096)
                     /locus_tag="WGQ85_000159"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0749674.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            7251..10100
                     /gene="uvrA"
                     /locus_tag="WGQ85_000160"
     CDS             7251..10100
                     /gene="uvrA"
                     /locus_tag="WGQ85_000160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0749675.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEQSITGQYLSSKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            10134..11729
                     /locus_tag="WGQ85_000161"
     CDS             10134..11729
                     /locus_tag="WGQ85_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ0749676.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(12036..12251)
                     /locus_tag="WGQ85_000162"
     CDS             complement(12036..12251)
                     /locus_tag="WGQ85_000162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749677.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFFPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(12280..13170)
                     /gene="sucD"
                     /locus_tag="WGQ85_000163"
     CDS             complement(12280..13170)
                     /gene="sucD"
                     /locus_tag="WGQ85_000163"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0749678.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(13181..14347)
                     /gene="sucC"
                     /locus_tag="WGQ85_000164"
     CDS             complement(13181..14347)
                     /gene="sucC"
                     /locus_tag="WGQ85_000164"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0749679.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(14446..14733)
                     /locus_tag="WGQ85_000165"
     CDS             complement(14446..14733)
                     /locus_tag="WGQ85_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749680.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(14828..16261)
                     /gene="lpdA"
                     /locus_tag="WGQ85_000166"
     CDS             complement(14828..16261)
                     /gene="lpdA"
                     /locus_tag="WGQ85_000166"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0749681.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            16458..16574
                     /locus_tag="WGQ85_000167"
                     /pseudo
     CDS             16458..16574
                     /locus_tag="WGQ85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16626..17807)
                     /gene="odhB"
                     /locus_tag="WGQ85_000168"
     CDS             complement(16626..17807)
                     /gene="odhB"
                     /locus_tag="WGQ85_000168"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0749682.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(17907..20735)
                     /locus_tag="WGQ85_000169"
     CDS             complement(17907..20735)
                     /locus_tag="WGQ85_000169"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0749683.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SVRAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADAYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQPIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(20940..22223)
                     /gene="gltA"
                     /locus_tag="WGQ85_000170"
     CDS             complement(20940..22223)
                     /gene="gltA"
                     /locus_tag="WGQ85_000170"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0749684.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(22335..22583)
                     /locus_tag="WGQ85_000171"
     CDS             complement(22335..22583)
                     /locus_tag="WGQ85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0749685.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(22587..23294)
                     /locus_tag="WGQ85_000172"
     CDS             complement(22587..23294)
                     /locus_tag="WGQ85_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0749686.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(23415..25178)
                     /gene="sdhA"
                     /locus_tag="WGQ85_000173"
     CDS             complement(23415..25178)
                     /gene="sdhA"
                     /locus_tag="WGQ85_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0749687.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(25181..25522)
                     /gene="sdhD"
                     /locus_tag="WGQ85_000174"
     CDS             complement(25181..25522)
                     /gene="sdhD"
                     /locus_tag="WGQ85_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0749688.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(25516..25893)
                     /gene="sdhC"
                     /locus_tag="WGQ85_000175"
     CDS             complement(25516..25893)
                     /gene="sdhC"
                     /locus_tag="WGQ85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0749689.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(26172..27575)
                     /locus_tag="WGQ85_000176"
     CDS             complement(26172..27575)
                     /locus_tag="WGQ85_000176"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0749690.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(27824..28561)
                     /locus_tag="WGQ85_000177"
     CDS             complement(27824..28561)
                     /locus_tag="WGQ85_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0749691.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(28840..31116)
                     /gene="metE"
                     /locus_tag="WGQ85_000178"
     CDS             complement(28840..31116)
                     /gene="metE"
                     /locus_tag="WGQ85_000178"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0749692.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(31254..32132)
                     /gene="metF"
                     /locus_tag="WGQ85_000179"
     CDS             complement(31254..32132)
                     /gene="metF"
                     /locus_tag="WGQ85_000179"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0749693.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            32333..32608
                     /locus_tag="WGQ85_000180"
     CDS             32333..32608
                     /locus_tag="WGQ85_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0749694.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            32608..32733
                     /gene="ykgO"
                     /locus_tag="WGQ85_000181"
     CDS             32608..32733
                     /gene="ykgO"
                     /locus_tag="WGQ85_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0749695.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(32841..33041)
                     /locus_tag="WGQ85_000182"
     CDS             complement(32841..33041)
                     /locus_tag="WGQ85_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749696.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            33191..34330
                     /locus_tag="WGQ85_000183"
     CDS             33191..34330
                     /locus_tag="WGQ85_000183"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0749697.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            34327..34908
                     /gene="metW"
                     /locus_tag="WGQ85_000184"
     CDS             34327..34908
                     /gene="metW"
                     /locus_tag="WGQ85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0749698.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            35025..35870
                     /locus_tag="WGQ85_000185"
     CDS             35025..35870
                     /locus_tag="WGQ85_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0749699.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(35920..36480)
                     /gene="efp"
                     /locus_tag="WGQ85_000186"
     CDS             complement(35920..36480)
                     /gene="efp"
                     /locus_tag="WGQ85_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0749700.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(36523..37671)
                     /gene="earP"
                     /locus_tag="WGQ85_000187"
     CDS             complement(36523..37671)
                     /gene="earP"
                     /locus_tag="WGQ85_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0749701.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(37690..37968)
                     /locus_tag="WGQ85_000188"
     CDS             complement(37690..37968)
                     /locus_tag="WGQ85_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749702.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            38470..39411
                     /gene="miaA"
                     /locus_tag="WGQ85_000189"
     CDS             38470..39411
                     /gene="miaA"
                     /locus_tag="WGQ85_000189"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0749703.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            39415..40134
                     /locus_tag="WGQ85_000190"
     CDS             39415..40134
                     /locus_tag="WGQ85_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0749704.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALSQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            40139..40627
                     /locus_tag="WGQ85_000191"
     CDS             40139..40627
                     /locus_tag="WGQ85_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0749705.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            40694..41446
                     /gene="rlmB"
                     /locus_tag="WGQ85_000192"
     CDS             40694..41446
                     /gene="rlmB"
                     /locus_tag="WGQ85_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0749706.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(41508..42899)
                     /locus_tag="WGQ85_000193"
     CDS             complement(41508..42899)
                     /locus_tag="WGQ85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0749707.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            43359..44234
                     /locus_tag="WGQ85_000194"
     CDS             43359..44234
                     /locus_tag="WGQ85_000194"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0749708.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALVRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            44243..45439
                     /gene="bamC"
                     /locus_tag="WGQ85_000195"
     CDS             44243..45439
                     /gene="bamC"
                     /locus_tag="WGQ85_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0749709.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKTPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(45496..46428)
                     /gene="pip"
                     /locus_tag="WGQ85_000196"
     CDS             complement(45496..46428)
                     /gene="pip"
                     /locus_tag="WGQ85_000196"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0749710.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT        11841 a        12577 c        11224 g        11019 t
ORIGIN      
        1 atggtattgt tttgtccgcc aaaatggacg cgaccgtata gggcgtcttc cgaacccatc
       61 aaccaacagc gctcataaaa acgcccgccc gagcctttgg attctttgta gataccgaaa
      121 ccgaaaactt cttcggcgag cattgacgcg gcgcgaataa aatcttcgtc ttccaaaaga
      181 cttacacgaa cgccgtattt atcgaaaaag ggtttttcat gaaatgaaaa gctgatttgg
      241 tcgatgaaag ccgaatcgga tacaccgcgc ctaagaggaa cgcctaacag gtttccttta
      301 ccgtccgtta tgtacatttc gtaacattcg aagacttcct gaaccctgcc tgccgtttcg
      361 gtttctgtac ccctcctgtt agataagggg ggaagatttg aagcggttgc cggcttcctg
      421 ccgtccgcta acgcgtccgt catcacgccg acaaccgcct ttgtcatccc ttgcttatct
      481 tccatggtgc gaatcctcaa aaacaggcaa aaaaaaagcc ctgttacttg tagaaagtaa
      541 aggacgttaa tttttgttaa tcgtcccttc ttagggacgc aatatataag gtttataccg
      601 tcgttgttcc taatgcgcaa tcagcgacat tgttgccaat tatccgaaag aaagttaagc
      661 ctgatggcat tgtatacacg gatacctttc gtagttatga tgtgcttgat attagtgaat
      721 ttagccattt acgtaagttt aacggcattc ccaaagagca tttggggctg catttaaaga
      781 aatgccaatg gcattttaaa tagaatctca aattcagatt ttaagacaat tagttaatgg
      841 gaatttggtc tggttatcta gtacagcccc aagtttttat ataaaaacag tttggtaagt
      901 tctcttcgtg aagtgcttat gttttgccac aaaagttcca taatcatggc ggcatcaccc
      961 aagctccatc tttccaagaa aatggcatct ttgttgtggt tgaaaaccga caaactcctc
     1021 ttttttcgat ttggccctgc ctgaatcgat ggcttcccgt gcggcagata tgccgtctga
     1081 aagcgaagca gcgacatttc cggcatatag ggcggcggcg gtattaagca atacgatatc
     1141 gcgcgcagcc ccttctttcc catccagcac ctcattcatt ttcaacaaag attcctgagt
     1201 attggcaact ttgatttcat ccaaattgcg gcgggtttcg ataccgaaat cttctgggcg
     1261 gatgtcgtat tcgctgattt ttccgtcttt gagttcggca acgcgcgttt tgcccgtcag
     1321 tgtaatttca tccaaaccgc cctccccgca aacgactaaa acgtgttttg aaccgagttg
     1381 ttgcaagacc cgcgacaaaa tgccgcacaa atcggtgtgg aacacgccca aaagctggtt
     1441 cggcgcgccc gcaggattgg tcaacggacc caatatgttg aaaatgcttc ggaaaccgag
     1501 cgaacggcgt accggggcga catggcgcat ggcactgtgg tgattggggg cgaacataaa
     1561 ccctataccg gtctggctga tactttgggc aatctgttcg ggagtcaggt tgaggtttgc
     1621 gcccatctgc tccatcacgt ccgccgcgcc gctggaagat gaaacggaac gcccgccgtg
     1681 tttggcaacc ttcgcgcctg ccgctgcggc aacaaacatc gaagtcgtcg aaatattgaa
     1741 ggttttggca ccatccccgc ccgtaccgac gatatcaacc agcccctccg cattctccag
     1801 cggcactttt gtcgcaaact cgcgcatgac ggctgcagct gcggtaattt cggaaacggt
     1861 ttcaaccttg atacgcaatc ctgtcaaaat cgccgcaatt tgttcgggcg gcacttttcc
     1921 gctcatcatt tggcgcatca agtcggtcat ttcatcgtaa aacaactcat tattgctgat
     1981 taatcgttcg atagcctgtt gcggtgtaat cattttttgt cctccgttca atattcggac
     2041 gaaaatgccg tctgaagggc ttcagacggc atcacgtcag attttttgcg gtttgaagtt
     2101 ttgaaattcg attaaaaaat tgtttaacat atcatgtccg tgctcggtca agagggcttc
     2161 ggggtggaac tgcacgccct cgacggcata ttccttatgg cgcacaccca taatctcgcc
     2221 gtcctcagtc caagccgtta cttccaaaca ttcgggcatc gtattccgat cgataacgag
     2281 gctgtgataa cgcgtacagg taaccggatt gggcaaaccc ttaaacatac ccttgcccga
     2341 atgggacacg ggcgacacct taccgtgcat cagcgttttg gcgcggacta tcctgccgcc
     2401 gaacgcctcg cctatcgtct gatgcccgag gcacacgccc ataatcggca gccggccggc
     2461 aaaatggcgc atcgccgcca cggaaatccc cgcttctttg ggcgaacacg ggccgggtcc
     2521 gataacgaga tattgcggat tcaatgcctc gatttcttcc aacgtaatat catcgttgcg
     2581 gcgcacggcg acttcctgcc ccaattcagc gaaatactgg acgatgttgt aagtaaaact
     2641 gtcgtaattg tcgataaaca aaagcatttt gtgattaacc tattgattta taatttgttt
     2701 gttattaact gcatttccga taccaccatc catacccctt atatcttagc gtgcccgatg
     2761 cgccctcgtg aacctgggca gagcctcgcg ttctgacaag tacgccattt tgccaccttt
     2821 tcgaccgttc gggaacgtgc cgtttttatg cactcataaa ttttggttat cccgcagccg
     2881 aacatagtgg gcaaacacag cggaatctgc gtcaacatca accgcgcccc cgaatgacgg
     2941 cggatacaaa tggacaccgc ccgatgccgc aaccgtttca tttgaaaaac ttttgcctat
     3001 gccctctgag tgttctcaaa atcttatgat ataaaaaatg taatgttata taataacaaa
     3061 cttttgaaat gcgacgagaa gccaaaatgg cacaaactac actcaaaccc attgttttat
     3121 caattctttt aatcagcacc cccatcctcg cccaagcgca tgaaaccgag cagtctgttg
     3181 acttggacga ggttaccgtc gtcggcaaaa gccgtccgcg cgccacatcc gggctgttgc
     3241 acacttcgac cgcttccgac aaaatcctct ccggcgatac cttgcgccaa aaagccgtca
     3301 acttgggcga tgctttggac ggcgtgccgg gcattcacgc ctcgcaatac ggcggcggcg
     3361 cgtccgctcc cgttattcgc ggtcaaacag gcaggcggat taaagtgttg aaccatcacg
     3421 gcgaaacggg cgatatggcg gatttttcgc ccgatcacgc cattatggta gataccgcct
     3481 tgtcgcaaca ggtcgaaatc ctgcgcgggc cggttacgct cttgtacagc tcgggcaatg
     3541 tggcggggct ggtcgatgtt gccgatggca aaatccccga aaaaatgcct gaaaacggcg
     3601 tatcgggcga actcggattg cgtttgagca gcggcaatct ggaaaaactc acgtccggcg
     3661 gcatcaatat cggtttgggc aaaaactttg tattgcacac ggaagggctg taccgcaaat
     3721 cgggggatta cgccgtaccg cgttaccgca atctgaaacg cctgcccgac agccacgccg
     3781 attcgcaaac gggcagcatc gggctgtctt gggttggcga aaaaggtttt atcggcgtag
     3841 cgtacagcga ccgtcgcgac caatatggtc tgcctgccca cagccacgaa tacgatgatt
     3901 gccacgccga catcatctgg caaaagagct tgattaacaa acgctattta cagctttatc
     3961 cgcacctgtt gaccgaagaa gacatcgatt acgacaatcc gggcttgagc tgcggcttcc
     4021 acgacgacga tgatgcacac gcacacaccc acagcggcag accgtggata gacctgcgca
     4081 acaaacgcta cgaactccgt gccgaatgga agcaaccgtt ccccggtttt gaagccctgc
     4141 gcgtacacct gaaccgcaac gactaccgcc acgacgaaaa agcaggcgat gcagtcgaaa
     4201 acttttttaa caaccaaacg caaaacgccc gcatcgagtt gcgccaccaa cccataggtc
     4261 gtctgaaagg cagctggggc gtgcaatatt tacaacaaaa atccagtgct ttatctgcca
     4321 tatccgaagc ggttaaacaa ccgatgctgc ttgacaacaa agtgcaacat tacagctttt
     4381 tcggtgtaga acaggcaaac tgggacaact tcacgcttga aggcggcgta cgcgtggaaa
     4441 aacaaaaagc ctccattcag tacgacaaag cattgattga tcgggaaaac tactacaacc
     4501 accccctgcc cgacctcggc gcgcaccgcc aaaccgcccg ctcattcgca ctttcgggca
     4561 actggtattt cacgccacaa cacaaactca gcctgaccgc ctcccatcag gaacgcctgc
     4621 cgtcaacgca agagctgtac gcacacggca aacacgtcgc caccaacacc tttgaagtcg
     4681 gcaacaaaca cctcaacaaa gagcgttcca acaatatcga actcgcgctg ggctacgaag
     4741 gcgaccgctg gcaatacaat ctggcactct accgcaaccg cttcggtaac tacatttacg
     4801 cccaaacctt aaacgacgga cgcggcccca aatccatcga agacgacagc gaaatgaagc
     4861 tcgtgcgcta caaccaatcc ggcgccgact tctacggcgc ggaaggcgaa atctacttca
     4921 aaccgacacc gcgctaccgc atcggcgttt ccggcgacta tgtacgaggc cgtctgaaaa
     4981 acctgccttc cctacccggc agagaagatg cctacggcaa ccgtcctttc atcgcacagg
     5041 acgaccaaaa tgccccccgt gttccggctg cgcgcctcgg cttccacctg aaagcctcgc
     5101 tgaccgaccg tatcgatgcc aatttggact actaccgcgt gttcgcccaa aacaaactcg
     5161 cccgctacga aacgcgcacg cccggacacc atatgctcaa cctcggcgca aactaccgcc
     5221 gcaatacgcg ctatggcgag tggaattggt acgtcaaagc cgacaacctg ctcaaccaat
     5281 ccgtttacgc ccacagcagc tttctctctg atacgccgca aatgggccgc agctttaccg
     5341 gcggcgtgaa cgtgaagttt taaaatcgga caggcaaaca aaaaaggttt tcagacggca
     5401 ttctgaaaac cttttttctt tttaatcaat ttgttaggaa atatggcaaa gacaaagaac
     5461 aaaccactat gccatcattc ccattttttt tgtttcatca ttcttacttt cacgccgtca
     5521 ttctcgcaaa agcagaaaaa tcaaaagcag aaacccaaga tcccgtcatt cccgcgcagg
     5581 cgggaatcca attcgttcgg tttcagccgt ttccgataaa tcactgtaac gttaagttcc
     5641 cggattccca ctttcgtggg aatgacggcg taaaggtttc tgttttttaa gttttaggta
     5701 actaataagt cgtcattccc gcgcaggcgg gaatccagtc tcttcgtttt cagccgtttc
     5761 cgataaatca ctgtaacgtt aagttcccgg attcccactt tcgtgggaat gacgcgtaaa
     5821 ggtttctgtt ttttaagttt taagtaacta atgagttgtc attcccacgc aggcgggaat
     5881 ccggtctctt cggtttcagt cgtttccgat aaatcactgt aacgttaagt tcccggattc
     5941 ccactttcgt gggaatgacg cggaaaggtt tctgtttttt aagtgttagg taactaatga
     6001 gttgtcattc ccgcgcaggc gggaatctaa ttcgttcagt ttctgttata gtggattaaa
     6061 tttaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggc
     6121 aacgccgtac tggtttaaat ttaatccact atatttctga taaatcaccg taacgttaaa
     6181 tttccagatt cccattttcg tgaacataac ggtgtgaaca ttagcaaaaa ttcaaaaaac
     6241 caaacttcct cattcgtgtg aatgatggta ctgatatttt ctatttcagt tcgcaaaatt
     6301 aatctttcaa cacatcttgc acagccgcct gaatcttagc cgccgctgcc tcgatttgcc
     6361 gttgttcggc agatgggttt gccgctgttt caaccggagc agtttgtaaa gcaggctcag
     6421 actcaggctc ggattcgatt tgcggcgcag tcagcggcaa acgcgccaat acagtgctaa
     6481 cgccagttac tttatcgcca atcgccactt gcgcctgcgc atcgacaggc agatacatat
     6541 ccacgcgcga accgaagcgg ataaagccat aacgttcgcc gcgggacagt ttcgcacctg
     6601 cttgggtgta gcacaaaata cggcgcgcca ccaaaccggc cacttgaaca aaagtaattt
     6661 cacgaccgga agccgtagtc gccaacaccg cattacgttc gttttccgtg ctggctttgt
     6721 ccaaatccgc attcacgaat ttgcctttgt tatagaccac tttcgttacc gtacaatcgg
     6781 caggcgattt ttgcgaatgc acgttgaaca cgttcataaa aatactgatt ttcaaagcat
     6841 cgacatcacg atacggatcg cgtgcgcgct cgaccaccac gatacggccg tcaaccggac
     6901 tcaacactgc ttcaggattt tgcggaatct cacgcgcagg gtcgcggaaa aactgcaggg
     6961 caaatacggt aaacacccaa aacggcaaag accaccagcc gcaacacatc gacaccagca
     7021 ggctcaaagc caaaccgccg ccaataatcg gccagccctc acgggcgata atcgggtggg
     7081 ggtaaagacg gttcatttgt catccttttg ttcaagcggg ttaaaattgc cgccattata
     7141 acggaatccg ccgcgttccg ccgacaaatg ccgtctgaac gcggcttcag acggcatccg
     7201 agaaagtgct acaataccag ttttccttta tttttcaggc ggcatccatt atgtacaagc
     7261 atcatcccca acattcgcac gacaacgaca ccatccgcat ccgcggcgcg cgcacgcata
     7321 atttgaaaaa cgtcgatttg gacattccgc gccacaagct cgtagtggta acaggcttat
     7381 cgggcagcgg caaatcgtcg ctggcatttg atactttata tgccgaaggt caacgccgtt
     7441 atgtcgagag cctttccgcc tatgcgcggc agtttttgca gatgatggac aaacccgatg
     7501 tcgatttgat tgaaggtctg tcgcctgcga tttctatcga gcaaaaatcc accagccaca
     7561 atccgcgttc caccgtcggc acggttacgg aaatccacga ctacctgcgc cttttgtacg
     7621 cccgcgtcgg cacgccgtat tgccccgaac acaatctgcc gttgtcgagc caaaccgtat
     7681 cgcagatggt cgatgccgtg ttgaagctgc cggaagacac gcgcgtgatg attctggcgc
     7741 cggcggtgcg cgagcgtaag ggcgagtttg tcgatttctt tgccgacttg caggcgcagg
     7801 gttttgcgcg cgtgtgcgtg gacggcgagg tctatcagct tgacgaagtg ccgaagctgg
     7861 aaaaaaacat caagcacaac atcgacgtgg tcatcgaccg cgtgaaagtg aaggcggaca
     7921 tcaagcagcg gctggcggaa agttttgaaa ccgcgctgcg ccacggcaac gagcgcgcgc
     7981 tggcgatgga gatggacagc ggcgaagaac attggttttc cgcgcgtttc gcctgccccg
     8041 tatgttcgta cagcctgccc gaattggagc cgcgcctctt ttcgttcaac aacccgatgg
     8101 gctcctgccc gacttgcgac ggcttgggta acaccaattt cttcgactcc gaaaaagtgg
     8161 tcgcgcatcc cgaattgtcg ctggcttcgg gcgcgattga cggctgggac aagcgcaacc
     8221 agttctattt ccaaatgatt cagtcgctgg cacggcatta cggtttcgac gtgcaggctg
     8281 cttgggaaac cctacctgca aaaatcaaaa aagtcgtgtt gcacggctcg ggcaaagaag
     8341 tcattgattt cacttacctg tccgaacgcg gcaccacctt caaccgcagc cacgccttcg
     8401 aaggcatcat ccccaatctc gaacgccgct accgcgaaac cgacagcgaa accgtgcgcg
     8461 aaaaactgcg cgaataccaa aaccaccgcg cttgcccgag ctgcggcggc gcacgtttgc
     8521 gcaaagaagc ccgctacgtt tacgtcggcg gcgagccgtt gcacgaagtt tccgcctggc
     8581 cgctgaccaa aacccaccga ttctttgaaa cgctggattt ggacggcaac aaaaaacaaa
     8641 tcgccgaaaa aatcctcaaa gaaatcaccg aacgtctcgg ctttttgatt aatgtcgggc
     8701 tggattacct gaatctctcc cgttccgccg aaaccctttc cggcggcgaa gcccagcgca
     8761 tccgcctcgc cagccaaatc ggcagcggcc tgaccggcgt gatgtacgtt ttggacgaac
     8821 cctccatcgg cctgcaccag cgcgacaacg accgcctgct tgccaccctc aaacgcctgc
     8881 gcgatttggg caacagcgtc atcgtggtcg aacacgacga agacgccatc cgcgaagccg
     8941 atttcgtcgt cgatatgggc ccgggcgcag gcgaacacgg cggcaacgta ctgattgccg
     9001 acacgcccga aaacgtcgcc aaatgcgagc aatccattac cggacaatac ctcagcagta
     9061 aaaaatccat tgccgtgccg tctgaacgca cgcccgtcaa tcccgaccgg atgctcgtcc
     9121 tcaaaggtgc gcgcggcaac aacctcaaaa acgttaccct cgaactgccg ctcggtttga
     9181 ttacctgcat caccggcgta tccggcagcg gcaaatccac cctgattaac gacaccctcg
     9241 ccaaaatcac cgcccgcgaa ctcaaccgcg cccaagaaga acccgcccca tacgacgaca
     9301 tccgcggtct cgaacatctc gacaaagtca tcaacgtcga ccaatccccc atcggccgca
     9361 ccccgcgctc caaccccgcc acctacaccg gcctgttcac ccccatccgc gaactcttcg
     9421 ccggcatccc cctctcgcgc gaacgcggct acaacgtcgg ccgcttttcc ttcaacgtca
     9481 aaggcggccg ctgcgaagcc tgccaaggcg acggcgtgat taaagtcgaa atgcacttcc
     9541 tgcccgacgt gtacgtcccc tgcgaagtct gccacggcaa acgctacaac cgcgaaacgc
     9601 tcgaaatcca atacaaaggc aaaaacatca gccaagtcct cgacatgacc gtcgaagaag
     9661 cccgcgaatt tttcgacgcc gtccccaccg tatcgcgcaa actgcaaacc ctgatggacg
     9721 taggtctcgg ctacatccgc ctcggccaat ccgccaccac cctctccggc ggcgaagccc
     9781 agcgcgtcaa actcgccttg gaactctcca aacgcgacac cggcagaaca ctctacatcc
     9841 tcgacgaacc caccaccggc ctacacttcg ccgacatcgc cctgctgctg gaagtcatag
     9901 gtcgtctgaa aggcaaaggc aactcgattg tgattatcga gcataatcta gacgtgatta
     9961 aaaccgccga ttggattgtg gacttagggc cggaaggcgg cgatggcggg ggcaggatta
    10021 ttgcttgtgg tagccctgag caggtggcga aagttaaggg gagttatact gggaagtatt
    10081 tgaaggtggt tttgaggtaa gtttaattca aaattaaata ggagattatt aagatgaaac
    10141 cgttaaaaac attagaattt ggatttgtgg atgctgcaaa ctacagaaga agagaaaata
    10201 aagatttatt taaccgaata tttgtaaaag gagaatattt ggatgaatta tgtgaaccaa
    10261 atatttcgtt tttaatcgga gaaaagggaa ctggaaagac agcatatgct gtttatttaa
    10321 ctaataactt ctataaaaac atacatgcca ctactaagtt tgttcgtgaa accgattatt
    10381 caaaatttat tcagctaaag aaagcaagac acttaactgt ttcagatttt acaagtattt
    10441 ggaaagtcat tttatatctg ttgatatcaa atcaaatcaa atgtaaagaa aacggaatat
    10501 tatcttcaat atttaataaa tttaaagcct tagatgaggc tataaatgaa tattattatg
    10561 gcgcttttga tccggaaatt gtacaagcaa taactttaat agaaaattca aaagaagctg
    10621 cggaaatgat ttttggaaaa tttgttaaac taggtgaaga ggaatcccaa caaataactt
    10681 ttacagaaag taaattccaa gcaaatttag gttttattga aagaaaattt aaagatgctt
    10741 tatctcagtt aaagctaaaa gataatcata ttttgtttat tgatgggata gatattagac
    10801 catcacagat tccatttgat gaatatcatg agtgtgtaaa aggtcttgct aacgccatat
    10861 ggatgttaaa taatgatatc ttcccttcca ttaaagatag taagggaagg atgagagttg
    10921 tgttattgat tagacctgat atctttgatt cattaggttt acaaaatcaa aataccaaac
    10981 ttcaagataa ttcagtattt ttagactgga ggacggatta taaatcttat agaagttcaa
    11041 agatttttgg cgtttttgat catcttttga gaacccagca agaaaaacaa gatagtttag
    11101 aaaaaggcaa ctcatgggat tattattttc catggaatgc tcctaattta catgatgagt
    11161 ataaaaattt aacttcattt attagcttcc taagaaaatc gtattatcga cctcgcgata
    11221 ttcttcagat gcttactttg ctacaaaaaa ataagaaaag taaggaagat tatgtcgtag
    11281 cagaagattt tgataatact tcttttcaaa gagaatactc gatatattta cttggtgaaa
    11341 tcaaagatca tcttttgttt tattatagtc aaagtgatta tcaaaatttc ctgaaatttt
    11401 ttgaattttt aaacgggaaa gatagattta aatatagtga ttttttaaaa gcatttgaac
    11461 gtttgaaaaa gcacttacaa acaacatcag tggaaatacc taaatttatg agtactgcta
    11521 atgagttttt gcaattttta tttgacttga atgttattgc ttatttagat aacccagaag
    11581 atgaaacgaa accatatatc cattggtgct ttaaagatag aaattatgca aatatttctc
    11641 ctaaaataaa aactgaaact gaatatttaa tattttcagg attatcaaaa gcccttgatg
    11701 ttggtactcc atttaagaac aaacagtaac ttgtatccca accaaccgtg tgcgctacgc
    11761 acacggttgg tagctgacgt gttgcagttt caacagagaa agccgtagcc tgcataccca
    11821 accgaccgcg tgcgtggctg tgccacacgc cctacctgtg aattacacgg aacttcaaag
    11881 ccaatacgag agctttgcaa aattcctttc cctcccgaca gccgaaaccc aaacacaggt
    11941 tttcggctgt tttcgtttca aatatccact aattttaccc caataccccc ttaatcctcc
    12001 caggataccc gataatcagg catccgggcc gccttttagg cgcaaacagg cacacttagc
    12061 ccgagacctt tgcaaaattc cccaaaatcc cctaaatgtc ttggtgggaa tttaggggat
    12121 tttggggaat tttgcaaagg tctctgactg ccccaaaagg gaaaaaataa tgtgggtatg
    12181 ttgaggctgc ctctttttga aatgacaaac aaaatgccgt ctgaaacccg atttccggtt
    12241 tcagacggca tttgatgttt aatcagttgt caaacctgat tatgccaaac cttttgcctt
    12301 caacacttcc agcatcgtcg tgcccaactc ggcagggctg cgagtatagg cgataccggc
    12361 tttttcgaaa gcggcgaatt tttcttcggc agtaccttta ccgccggaaa taatcgcacc
    12421 ggcgtggccc atacgtttgc ctttaggagc ggtaacacca gcaatgtaac ctacaacagg
    12481 tttggttacg ttggattgga tgtattcggc tgcttcttct tccgcagtac cgccgatttc
    12541 accaatcatg atgatggcgt cggtatctgg gtcttcttgg aagagtttca gtgcgtcgat
    12601 ttggttcata ccaggaatcg ggtcgccgcc gataccgata caggttgatt gacccaagcc
    12661 cagtttggtg gtttgtgcca cggcttcgta agtcaatgta ccggaacggg aaataatacc
    12721 gatgcggccc ggagtgtgga tgtggcccgg cataatgccg attttgcact cgcccggagt
    12781 aatcacgccc gggcagttag ggccgaccaa gcgggtaccg ttgccgttgg tttccaagta
    12841 acgtttggct ttgagcatgt ccaaagtagg tacgccttcg gtaatcacaa cgaccaagcc
    12901 tacgcctgaa tcaacggctt caacgataga atccaacaca aacggagcgg gaacgtaaat
    12961 tacggatgca tccgcgccgg tttctttaac agcttctttc atggtgttga atacaggcag
    13021 gttcaggtgg gtttgaccgc ctttgcccgg ggtaacgccg ccgacaactt tagtgccgta
    13081 agccagagct tgttcggagt ggaaagtacc gtttttaccg gtgaaacctt gaaccaatac
    13141 tttggtgtct ttattaatca atacgctcat tcttttctcc ttaggcgttt acggctgcaa
    13201 caattttttc ggctgcgtca ttcaggccgt ctgcagaagt cagtttcagg cctgattcgt
    13261 tcaggatttt cgcgccgagt tcggcgttgt taccttccaa acgaacaacg acaggaacgt
    13321 cgacgttgat ttctttaacg gctgccacga tggcttccgc aatcatgtcg caacgtacga
    13381 taccgccgaa gatgttgatc aatacgcctt taacggattt gtcttccaaa atcagtttga
    13441 acgcttcaac cacgcggtct ttggttgcgc cgccgccaac gtccaagaag ttggcaggtt
    13501 gaccgccttt gagtttgatg atgtccatgg tggccatcgc caaaccggca ccgttaacca
    13561 tacagccgat gttgccttcc agggcaacat agttcaggtc gaattcagaa gctttcagtt
    13621 cacgttcgtt ttcttgagat ttgtcgcgca attcagcgat ttttggcagg cggtagagcg
    13681 cgttgctgtc gatgccgatt ttgccgtcca cgcaagccag tgcgccgttt tcgcgaactg
    13741 ccagcgggtt gacttcaaac agggcgaagt cgttttcgac aaacgctttg tacgcgccgg
    13801 tcatcagttt gacgaactcg ttgatttgtt tgtctttcaa gcccagttgg aaggcaactt
    13861 cgcgagcttg gcaaggttgc aggccgacca gcggatcaac ggttactttg aagatttttt
    13921 ctggagtttc ggcagcaact ttttcgattt ctacgccacc ttcggtagaa gccatgaatg
    13981 taacgcggcg ggtagaacgg tcaaccactg cgcccaagta cagctcggtt tgaaccggat
    14041 acatgtcttc gcaaaccaaa acgctgttga caggttggcc gttggcatca gtttggtaag
    14101 ttaccaagtt ggtgccaatc aggctttcag ccacttcttt agcttcttcg cggcttttaa
    14161 cgacttttac gccgcccgct ttaccgcggc cgccggcgtg tacttgtgct ttgacaacag
    14221 cgaatttgcc gcccaatttg tcgtaagctg cagcggcttc ttcgccgttg tgtgccaaaa
    14281 taccgccttg tacgggcaaa ccgtagctag ccagcagttc tttagcctga tactcgtgta
    14341 aattcatgga tttctccttt agattagtgg aatgccccga gtttgcctcc aaatggcaag
    14401 cccgtgcgtt tcagactacc tttcggcggt cggaaaatcg tcgtttcact tgctgaaagg
    14461 caaccacatc cgcgccagca gatacatggc tgcaccgcct cccccgagaa taaaaaagac
    14521 gatgcctttt ttacgcgaat tcggacagag cagataaacg gcaaggctga aactgataaa
    14581 caacaatgcc acgcgccaaa ccaattcttt atctttgaaa cggttgagcg tataagtttc
    14641 ggtcatcatc acatacatct gccacaatgc ggccatacac atgctgatga tgcccgccgg
    14701 tataaagaaa ataccgacta tttctttgtt cataatgaat gtaaatgctg tttgtttgtc
    14761 tgacggaatt ataaatcaga aaacgaagct ctcggaacat tcaatttgtc ggaggcctta
    14821 atgtctatca accgtgcaaa gcgcgtttgt cggccgccaa tgcagcttcg tgaaccactt
    14881 cggacaaggt tgggtgggca tggataatgc gggcgatgtc ttcgctgctg gcgaagaatt
    14941 cgagcgcagt cacgccttcg gtaaccaatt cgctgacaac cggaccaatc atgtgtacgc
    15001 ccaagatgcg gtcggttttg gcgtctgcca acactttaac cgtacctttg gctttgccca
    15061 ttgccaatgc gcgaccattc gcaccaaaac ctgaagtacc ttttttgtac tccacgcctt
    15121 cggctttaag ctgttcttcg gttttaccca cccaagcgat ttcaggatcg gtgtaaataa
    15181 caaacggtac gttgttgaaa tcgatatgcg gtttttgacc ggcaatgcgc tcggcaacgg
    15241 caacgccttc gtcgctggct ttgtgtgcca acatcgggcc gcgaaccacg tcgccgattg
    15301 cccatacgtt aggcaggttg gtacggcatt cgccatctac tttgataaag ccgcgctcgt
    15361 ctttttccaa gcctacggct tccgcgttca ggcctttggt gtttggaata cggccgatgg
    15421 caacgatcag tttgtcgaat acttcggttt tggcttcgcc ggcagcagtt tcgtaagcaa
    15481 cggaaacacc tttgccttca gacttgatgt cgccgatttt cacgcccaat tcgatgctca
    15541 gaccttgctc tttggtgaag tatttgaagg cttctttggc gatttgttgg tcggcggcag
    15601 ccaggaaggt cggcgcggct tcaagaatgg taacttccgc acccacgcgg ttccatacgg
    15661 aacccatttc caaaccaatc acaccggaac cgatcacgcc gagtttggca ggtacttcgg
    15721 tcaggttcaa tgcgccttcg ttgtccaata cgttcacatt gtcgatagcg acttgtggca
    15781 gcggacgcgg tacggaaccg gtcgctacga tgacgtgttt ggcttcgata acggttttct
    15841 cacctttgtt atcgacttcg atttggtaag catcgccatt tttgccggca aaggaagccg
    15901 taccgaacag gctggttact ttgtttttct ggaacaggaa tttcacgccg ccggtcagtt
    15961 tggttacgat ggcatctttg cgctcaatca ttttggccac gtcgaatttt acgtcgccga
    16021 cagtgatacc gtgttcggca aactcgtgtt gcgctgcgtg gaaatgttcg ctggattgca
    16081 acagggcttt agaagggata caacctacgt tcaagcaagt accgcccaat gcaggggcat
    16141 tgcctgcttt gttaacgcct gcatcgacac aggcagtttt gaaacccagt tgtgcggcac
    16201 ggatggcggc aacgtatccg cccgggcctg caccaatcac tactacatca tattgagaca
    16261 tcgtgtcatc ctttgctttt agcagtatct caaaataaaa acgatacttt tcgtttcttt
    16321 tttattttga atgactgcgt tttgatggtt ttaagaaaaa gccgtctgaa agtccatatc
    16381 tgccgattca ttcgaatcaa atgtttcaga cggtctcaat cgtttgttga gcctattgta
    16441 gcacgatact acattacgct acatctaaat atagtggatt aaatttaaat caggacaagg
    16501 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggttta
    16561 aatttaatcc actataaaaa ttcagacggc cttttcatta acaaataaaa ggccgtctga
    16621 aacgattaca gatccaacaa caggcgggct gggtcttcca acgcgtcttt aatggctacc
    16681 aaggtcaata cagcttcgcg gccgtcaatg atacggtggt cgtaagacag agccagatac
    16741 atcattggac ggacaacaac ttggccgttt tcaaccacag cgcgctcttt agtggcgtgc
    16801 atacccaaaa tcgcagattg aggcggattg atgatcgggg tggacatcat agaaccgaaa
    16861 gtaccgccgt tggtaatact gaatgtaccg ccggtcagat cttcgatagc gattttgccg
    16921 tctttggctt ttttcgcgta atcaacaatt gcttgttcga tgtcggcaat gctcatttgg
    16981 tcggcatcgc gcaggattgg cacaaccaaa ccgcgtgggc tgccaatcgc gataccgatg
    17041 tcgaagtagc cgtggtacac aatgtctttg ccgtcaacag aagcattcac aaccgggtat
    17101 tttttcaggg ctgcaacagc ggctttaacg aagaaggaca taaagcccag tttcacgccg
    17161 tgttctttct cgaatttttc tttgtacttc gcacgcaagt ccatgattgg tttcatgttg
    17221 acttcgttga atgtagtcag aatggcgttt tcttgttgag aagccaagag gcgttctgca
    17281 acacgggcac gcaggcggct catcggtacg cgttcttcag gacgtgcgcc ggcaggaagt
    17341 gcaacggcag gagcagcggc tgcggcaggt ttggctgcgg cattttgtac gtcttctttc
    17401 aatacgcgac cgtcacggcc ggaaccttgc aatgcgttca cgtcaacacc gctctctgca
    17461 gccagtttgg cggcggcagg catagcggcg ttgttttgtg cagcagcagg agcggcggcc
    17521 ggggcagctt cggcaggagc ggcggcaggt gcttcagcag cggcagtagc agcggtatcg
    17581 atacgtgcca aaacttggtc ggcaacaacg gtttcaccgt cttgcgctac gatttcaacc
    17641 aatacgccgg cttgtggaga aggtacttcc aaaaccactt tgtccgtttc gatatcgatc
    17701 aggatttcgt cacgggcaac tgcttcgcca acttttttct tccattccaa gagcgtgcct
    17761 tcagatacgc tttcagacag cataggtact tttacatcaa taatcatttt gtgtctccaa
    17821 tggccctttc agacggcctg ttgtgtgttt ttcattctgc atatgggtgt tccgcctgag
    17881 cagcctcaga cggaatgctg cttggtttac aacgccaaag cgtcttcaac caattgtttc
    17941 aattgagcaa tgtgtttgct tgagtagccc actgcaggcg atgcgctgct tggacgaccg
    18001 gcataagaca gtttttgctc ttcgctgata acgtcttcga tgcggtggcg gatttggtag
    18061 aacgcgcctt ggtttttcgg ctcttcttgc gcccaaacca cagattttgc gttcggatat
    18121 ttcgccagtt cggctttaac ctcatcgtat gggaacggat acagctgctc aacgcggaca
    18181 atcgcaacat catcttccag tttacgttcg gctcgaccgg cttccaagtc atagtaaacc
    18241 tgaccggcac acaataccac gcgtttcacg ctgtcgttgc ttgcgcgttc ggcggtatcg
    18301 ccgacaaccg gacggaaagt cgaaccttcg gtgaagtttt ccagcgggct cattgcccct
    18361 ttgaatcgca acaggcgttt ggacatgaaa attaccagcg gtttgcggta tgaacctaag
    18421 acttgacgtt gcaagaggtg gaacatttgc gacgcttcag atggcatgat gacttgcata
    18481 ttgttctcag aacacagttg caaccagcgt tctacgcgtg cagaagagtg ctcagggcct
    18541 tgaccgtcgt aaccgtgcgg caggatggtg gtcagaccgc acaaacggcc ccatttggtt
    18601 tcgcctgaag acaggaattg gtcaatcgtc acttgcgcgc cgttggcgaa gtcaccgaat
    18661 tgagcttccc aaatggtcag tttgtcaggt gcggagcagg caaagccgta ctcgaacgcc
    18721 attactgctt cttcgttcaa aatggagtcg ataaccaaga actcgcccat accttcgccc
    18781 atatggcgca gaggaacata agtaccgtca tcccattttt cacgtttttg atcgtgcaat
    18841 acggcgtggc ggtgcgagaa cgtaccgcgg ccggagtcct caccggagat acgcacgcca
    18901 tgacctttgg ttacgaggct ggcatatgcg agggtttccg ccatacccca gtcgataggc
    18961 tgtttgccgg atgccatggc tttacgcgct tcaatcacac gttttgcagt cggatgcagg
    19021 gcaaagcctt ccggtacggc ggtaaacttc tcggtgagac gctcaatatc tgcggcaggt
    19081 aaaccggttt cgatgtgttc gcgccaatct ttaccttgat atttgctcca atcgatttgt
    19141 gtgcgttgga aattgctcaa agttgtttgc tcgacgtgtt cgcctttgtc caaagcatca
    19201 cggtaggctt ggatgtaagc atcagcttct gcttgagtta ccacgccttc ggcaatcagt
    19261 tgctcggtgt acaaagcacg cgcaccgggg tgttgcgata cttttttgta catcatcggt
    19321 tgggtcaagg tcggatcatc gccctcgttg tgaccccatt tacggtagca gacaacgtca
    19381 atcacgatgt ctttatggaa ttttttgcgg taatccaaag cggcttggat ggcaaagcaa
    19441 acgcgttcgg gatcatcgcc gttgacgtgg ataaccgggg cggaaaccat tttcgcgata
    19501 tcggtacagt gtacggttga acgggtatcg cggatatcgg aagtggtaaa gccgatttgg
    19561 ttgttgatga cgatgtgaac cgtaccgccg gtggtataac cgcgcgtttt agacaggttg
    19621 aatgtcgctt ggttgacacc caaaccgata aatgcggagt cgccgtgaat caatacgggc
    19681 aagactttat cgcggccgtt ttcgcccaaa cgtttttgct tggcgcgtac agaaccttcc
    19741 actaccggat tgacgatttc caagtgtgac gggttgaatg ccaaagaaac gtgcatcgga
    19801 ccgtgcggcg tggcaatatc ggagctgaag cccatatggt atttcacgtc gccgctgggc
    19861 agtttgattt cggcacggcc ttcaaactca gcaaacaaat cgccgggttt tttgcccaaa
    19921 atgttcacca aaacattcag acggccacgg tgcgccatac cgatgatgac ttcttccacg
    19981 ccgtctttac cggcgttttg aatcaggtag ttcaaaccgg caatcgcgct ttcgccgcct
    20041 tctacaccaa agcgtttttg gccgacatat ttggtatgca ggtagcgttc caaagtttcg
    20101 gcagcagtca tctctttcaa gatacggcgt ttttgatcgg cattgtaatg cggtgtggac
    20161 aatacgcttt caaaataatt gcgtacccag cggcgctctt cggtattggg aatatagata
    20221 tattccaatg cgatgtggcc gcagtaggtt tgtttgaggt tgctgatgat ttgggacaaa
    20281 ggcagtttgc cgcgattggc aaaatcgccc tcgcccatat tgaattgaag cgtcatatcg
    20341 gcatctgaca gaccgtggaa tttcggatcg agggcttcaa tatcgcgcgg ggggatacgt
    20401 ttgagcggat caagttgggc tgcacccacg ccttggatac gataggcgga aatcagccgt
    20461 aaaacgctga cttgcttttt cagcattgcc tcatccgcac cgcccgcaac ggcagatgca
    20521 attttctttt tcgccaaagt aacaaatgat tcgcgaatcg gtgtgtgtgc gacatcgaca
    20581 gcaaccgtcc ccggctgttt gctcaaatcg gtgaaatact gcttccattt ttcatcaacc
    20641 gcatcggggt tttccaaaaa agcctcgtac aattcctcaa tgtaaggtgc gtttgaaccg
    20701 aacaggtaag agaaattgag tttttcgtcc atcatggcgt gcgctctttt ctttaaaata
    20761 taaacaaagg gtagaagtcc gcgtcctgac ggctaaactg atgccgcctg aaaagacatt
    20821 ttattctacc ctattttaaa ccggattgct tgtcttgatt tcatccgact acaatcagac
    20881 ggattcggga aacaatccgg ttctctgata ttgcctgttt gacaatatat taatgtatgt
    20941 tacctctcgc ctggcggcac ataatcgcgg cgttccgaac cagtataaag ctggcgcggg
    21001 cggccgattt tcagcgaagg atcgctaatc atctcgtgcc agtgcgaaat ccagcccacg
    21061 ctgcgcgaca gggcgaagat gacggtaaac atttcggtcg ggatgcccag cgcggacagg
    21121 acgatgccgg aatagaaatc gacgtttgga tacagtttgc gttcgataaa gaacgggtct
    21181 ttcagcgcaa tctgttccaa ttccatcgcc agtttgaatt tcggactgtc ttccaagccc
    21241 aattccttca aaacttcata gcaggtttcg cgcataatgc tggcacgcgg atccatattg
    21301 cggtacacgc ggtgaccgaa gcccatcaga cggtatttgc gttgtttcac accttccatg
    21361 tatgcggcaa cattagacac atcgccgatt tcgtccaaca ttttcaacac ggcttcgttc
    21421 gcaccgccgt gcgaagcacc ccacaggcag gcgataccgg cagcaataca ggcaaacggg
    21481 ttcgcacccg aagaccctgc cagacggacg gttgaagttg aggcgttttg ctcgtggtcg
    21541 gcatgcaaaa taaagatgcg gtcgagcgcg cgtgccaaaa cgggattggg tttgtagtct
    21601 tcacacggcg tggcgaacat catatgaagg aagttttcgg aataagaaag attattcttc
    21661 ggataattga acggcagacc gtttgaatag cggtagcaca ttgccgcaat ggtcgggatt
    21721 ttagaaatca ggcggtaaat cgcgattttg cggtgttcgg gattgctaat gtccaagctg
    21781 tcttggtaga acgcagacag tgcgccgacc acgccgacca tcatcgccat cggatgcgcg
    21841 tcgcggcgga acccccggaa gaaccaagtc agctgttcat gcaccatcgt gtggcggcgg
    21901 acggtattgt caaattctgc cttttgctcg ggagtcggca gttcgccgta aatcaacagg
    21961 tagcagactt ccaaataatc ggacttttcg gccagctgct cgatggggta tccgcgataa
    22021 taaagcaagc cttgatcgcc gtcgatgtaa gtaattttag actcacagct tgcggttgaa
    22081 acaaatccgg ggtcgaagga aaacaaacct gtattttttg tcagcccccg aatgtcaacc
    22141 acatcgtgcc cgatgctggc ttccaatacc ggcagctcca aacctgcctg acccggtacg
    22201 ttgagtttga ttgatttgga catattattg ctcctttgca agttataacc cacgttactg
    22261 catccgcagc acgttctttg ccttcagacg gcttttgcat tttgaaatct gcctgcaggc
    22321 agatttcaga tgcctcatgc ccgtctgatt ttttcaagca tcgggataag gtgccctttg
    22381 tccgtttccg aatgcccgtt aatcaaggca agcaattctt gatcttgaaa ttcaaggatt
    22441 tcggaaaact cggacagctc tttatcgctc aaatgctcga attctttttc cataaacctg
    22501 ccgaagatta aatctaattc caacaatccc cggcgggttt gaaaacggat tttccgtttg
    22561 gcaatatcgt caaaaaccat catttcttaa acggcccgtt tcaacataat ctctttaatc
    22621 ttaccgatgg ctcgggtcgg attcaagtgt ttaggacata cgtctacgca gttcataatg
    22681 gtgtggcaac ggaacaaacg gtatgggtcg ttcagattat ccaaacgctc attagtgatg
    22741 gtatcacggc tgtccgcaat gaaacggtaa gcattcagca aaccggacgg accaacgaat
    22801 ttatcagggt tccaccaaaa tgacgggcag gcagtcgaac agcaggcgca caaaatacac
    22861 tcgtacaaac cgtccaactc tttacgctct tcctgagttt gcagacgctc tttgtccgca
    22921 tcaatcggat tatcgttgac aacataaggt ttgacggaat ggtattgttt gaagaactgg
    22981 gtcatatcca caatcaggtc gcggataaca ggcagacctg gcagaggacg gattttaact
    23041 ggctgtttca agccacgcag atcggtcaaa cacgccaagc cgtttttgcc gttgatgttc
    23101 ataccgtccg atccgcaaat gccttcgcgg caggagcggc ggaaagacaa ggtatcgtct
    23161 tgtgctttca ggcgtaccaa agcatccaaa agtttcacgt cggtcggttc caattccaac
    23221 tcgtaacgct gcatataagg cttggcatca acatccgggt tgtaacggta aatttcaaaa
    23281 ctcatttttt ccatgagaag tgttcctttt ctcatcaact gcccaatgat tttgtattat
    23341 aaattcaagc agttcaatgt gggtatggtt agatttcaga cgagcttatg aggcgaaaac
    23401 cgtctgaaaa cgcatcaata aacgcgcttg gccggtttga tgtattccac gctcaaaggc
    23461 ttggtatgta ccggtttgta agacaaagta ttggtatctg aatggtacag cgtatgtttc
    23521 atccagtttt catcatcgcg ctcaggatgg tcgtctgaag cgtgcgcacc gcgtgattct
    23581 ttacgtgctt cggcagacac caaagtcgct ttcgccactt caattaggtt atccaattcc
    23641 aaagcctcga tacgcgcggt attccacact ttgctcttgt ctttgatttc ggtacgtttc
    23701 acacgctcgg caatcgccat gacttctcga acgcctttgc tcagaatctc atcagtacgg
    23761 aacacgccgg cgtgcaattg tacggagcgt tgcagttcgc ggcgcaatgc atcaacgttt
    23821 tcaccatcgg tttggctgtc caaacgctcg atacgttggc gggtcaactc accagcatta
    23881 gcaggcaaag gtttccagtc gctttgctct ttgatgaatt taatcatgct gtcgccggca
    23941 gctttaccga ataccaccaa gtccagcagg gagttcgtac ccaaacggtt cgcaccgtgt
    24001 acggaagcac aggcgcactc acctgcggca tacaggcctt ttacaggcac ttcgtattcg
    24061 tctccttgcg gcacaacgac ttcgccgtgg taattggtcg gaatgccgcc catcatatag
    24121 tgggtagtcg gcacaacggg aatcgggtct ttaatcggat cgataccagc gaactgaatg
    24181 gaaatctcgc ggatgcccgg cagtttttcc ataatttttt ctgcgccgat atggtcgatt
    24241 ttcagtaaga catggtcttt gtttttaccg caaccgcgac cttcgtagat ttccatcgcc
    24301 atcgcgcggg aaacaacgtc gcgagaagcc aagtctttta cggtcggcgc atagcgttcc
    24361 ataaagcgtt cgccgtcggc attcaacaga ataccgccct cgccgcgtac gccttcggta
    24421 atcaacacgc ccgcacctgc cacgccggtc gggtggaatt gccagaattc catgtcttcc
    24481 aacgggatac ctgcacgcgc acaaataccc aaaccatcgc cggtattcat ataggcattg
    24541 gtagaagacg cataaatacg gccgccgccg ccggtagcaa acatcacagc tttagcgtgg
    24601 aaaatataaa cttcgccggt ttccatttcc atggcggtta cgccgacgac atcgccgttt
    24661 tcatcacgaa tcaaatcttg tgccgtccat tccacaaaga attgcgtatt ggcacggaca
    24721 ttttgttggt acaaagtatg cagcatcgca tgacctgtac ggtcggcaac cgcacaggcg
    24781 cgttctaccg cgcgtttacc gtgttcggca gtatggccgc cgaaaggacg ctgataaatt
    24841 ttaccgcttt ccacacggtc aaaaggcata cccatgtgtt ccaactcaat tacggcttca
    24901 ggcgcggcgc ggcacataaa ctcaatcgca tcttggtcgc ccaaccagtc ggaacctttc
    24961 acggtatcgt acatgtgcca gtcccaacgg tcttcctgca cattacccag agaggcggaa
    25021 ataccgccct gcgccgctac ggtatgcgaa cgggtcggga acactttaga caaaacggca
    25081 caattcagac cggatttgga taattggagg gctgcgcgta aacctgcacc accaccgccg
    25141 acaatcacgg catcaaactt gcgaacagga aaacccatac ttacccccaa attactttaa
    25201 ttgaatacac caagcagccg accagccaga cgatggtggc aacctgcaaa aacaaacgca
    25261 cgccgaaggg tttgatatag tccatccaca aatcgcggat acccacccaa gcgtgcaaga
    25321 atacggcgat gaagctcact tgggtaaata cttttaccca agtttgacta aaaaatgcct
    25381 gccatgccga atattcttta ggcagggaaa atagaaccac taaaagtgca acggtataaa
    25441 tcaacataat aaccgcagtc gcacgttgca tcgcccaatc gcgcaaaccg taatgggcac
    25501 cggtcaattt acgttctacc ataacaacgc tcccaaaacg acagtcaaaa ccaatgcaga
    25561 agcaaatacg gctttagcgg tattgcgcgc agtattcagc tcaaggcctt tgtgtgcatc
    25621 caaaaataaa aagcggatac cggcgagaga atggtgcaga taagcccaca atacaccgat
    25681 taaaaccagc ttgaccaaag gatgggaaac aatggcacgg taagtttcaa atgcagactc
    25741 ttgactcagg gtaccggaca ggaaatacag caggaaaggc agcataataa acagcccgac
    25801 cccgctgatg cggtgaagga tggaaactat cccgggtatc ggcagacgga tgttcggcaa
    25861 atccagataa acaggacgcg gtttggcaga catagtcaat tcctctgtag ttatatatcg
    25921 actcaaaaac cgtaaaccca ttgctacact gggttacata aacgttaatt tacctgtttt
    25981 atcccatttt gaacagtgta ttttaaaaac aaactgatag attttgacaa acacgcgtgg
    26041 aaacagcaga ttaaaagaaa gatttttagt tttgccgccc ccttcccgac acggtatcag
    26101 catccgaccc catccccaaa aacaaaatgc cgtctgaaaa aatttcagac ggcattgttt
    26161 tgccatatcg gtcaggcttt caatttcgca tcggcatcgc gcaacgcggt acgcagacct
    26221 tcctcgataa cgggatggta gaacggcata tccagcattt gcggaacggt catcttcatt
    26281 tgatgcgccc atgccaacaa gtgcgccaaa tgttcggcgg cagggcctac gatttccgcg
    26341 ccgataaagc ggccggtggc tttttcggca tacaggcgca tatggccttt gttcaccagc
    26401 atcacgcggc tgcggccttg gtttttgaac gatacttcgc cgatgacaaa ttcgtcggct
    26461 tggtattgcg cggcaacctg cgcgtatttc agaccgacaa agccgatttg cggactggta
    26521 aacaccacgc cgatggtgct gcgccgcaaa ccgccgccga tattcgggta acggcctgca
    26581 ttgtcgccgg caatcttgcc ttggtcggca gcttcgtgca gcaggggcag ttggttggac
    26641 gcgtcgcccg caataaagat atgcggaatg ctggtctgca tggtcagcgg gtcggcaacg
    26701 ggtacgccgc gcgcatcttt ttcgatattg atattttcca aaccgatatt gtcaacgttc
    26761 ggacggcggc ccacggctgc cagcatatat tcggcaacaa atacgccttt ttcgccgtcc
    26821 tgctcccaat ggacttctac attgccgtct gcatcgagct tgacctcggt tttagcatcc
    26881 aaatgcagtt tcaattcttc gccgaacacg gctttcgcct cgtctgaaac gacggggtcg
    26941 gaaatgccgc cgatgattcc gcccaaaccg aaaatttcaa ctttcacgcc caaacggtgc
    27001 aatgcctgac ccagttccaa accgataaca cccggcccga acacggcaac gcgcttaggc
    27061 agcgtatccc atgagaaaac gtcatcgttg ataatcaaac gattgcccaa agactgccat
    27121 tgcggcagga tgacgggacg cgaaccggta gcaatcacga aactttttgc cgtaatttga
    27181 gtatgctcgt cgatttggac ggtatgctcg tcgataaatt tagccgaacc catgatgcgc
    27241 ttgtcggcag gccactcttc cacatcggca acgacaaagc cgacaaaacg gtcacgctcg
    27301 gatttgacgc gctgcatgac ctcttcgccg ttgacgacga tgctgtcttt gtccaaatgc
    27361 acgccgaacg ggtcggtatg caatgcgtga tgacgcgcct ctgcggcggc aatcaagagt
    27421 ttggaaggca tacagcccac gcgcgcgcag gtcgtgccga acacattgtt ttcaatcagg
    27481 taaacattat ccgaatgtaa acgggcattg cgaaacgcac ccataccggc agtaccgccg
    27541 ccgattacga cgacatccgc ttgaattttt ttcataatct ttgtccttcc gttttcgtct
    27601 gaaacattgc cgacacagaa catatcggtt tcagacggca tttaaatatt ttcccaatca
    27661 aaaacatcat tcagataaat aaaaacaaaa tttcgattga gtgcaacaat atccaaattc
    27721 tcttataaat atagagttat tcggagcggc agtgtagaac cgcccgaata actctatccg
    27781 gtatatcaga cagtttaatc aaaccgcctt aagcaaacag ctatcagttt ttagccaagt
    27841 aagcttccaa atcttcgctg ccgccgatgt atttgccgcc gatgaagact tgaggggcag
    27901 tcatcttgcc ggtaatggcg cgaacggaag taacggttgc atctttgccc aatacgattt
    27961 cttcgtaaga caaacctttg tcttgcaaag cttgtttggc tttagcgcag aattggcaac
    28021 ctggtttagt gaaaattgcc acagactctt gagccttcca atcgggagca acgaattgca
    28081 gcatagtatc tgcatcggat actttgaacg gatcgcccag ttcttcaggt tcgatgaaca
    28141 ttttttcaac cacgccgtcg ttaaccagca tggagtaacg ccaagagcgt ttaccgaagc
    28201 ccaagtcttc tttaccgacc agcataccca taccttcggt aaattcgccg ttgccgtcag
    28261 gaatcatgta gatgttgtcg gattcttctt cggcagccca agcgttcatt acgaacgtat
    28321 cgtttacaga tacgcagtag attgcgtcaa cgccgttttc tttgaacgcg ccgaacaatt
    28381 cgttgtaacg cggcaggtgt gaagaagaac aagtcggggt aaatgcaccg ggcagggaga
    28441 atacgactac ttttttgcct ttgaacaaat catcggtaga cacatctttc caagtgtcgc
    28501 cgacgcgggt gcggaatact acggaaggta ctttttgacc ggtacgatct tgcaaagcca
    28561 ttttaagctc cttaatagat tggtttgaga aataattgtg aatcgctggg cattctaccc
    28621 catgcaaaat gatttgtata atttatagtt caaataatat aaattattta aaactatcaa
    28681 acacaactcc gatttgcctg tttcccacat ataaagccga attagaaaaa aacaagctgc
    28741 aatgaaaaga ttttccggca actgcgccct tgcgccgcaa atcaggacaa aataccgtct
    28801 gaaaggcttt cagacggtat ttattcatac ggtctcggct tatttcgcca attcggcacg
    28861 cagttttcgg gttacgttca tcatcacttg gagttgttcc agagtttctt tccagccgcg
    28921 tgttttcagg ccgcagtctg ggttaaccca cagacgttca aacggtacaa cctcgatggc
    28981 tttgcgcaac aggtgctcaa cttcggcttc agtcggtacg cgcgggctgt ggatgtcgta
    29041 aacgcccggg ccgatgtcgt tcgggtattt gaattcgccg aatgcggtca acaattccat
    29101 gtcagaacgt gaagtttcga tggtaatcac gtccgcgtcc atgctggcga tggccggcag
    29161 gatgtcgttg aattccgagt aacacatatg ggtgtggatt tgggtactgt cttcgcaacc
    29221 ggttgacgac aggcggaagg cttcgcttgc ccaagccaag taggcatccc aatcggcacg
    29281 tttcaacggc aggccttcgc ggatggccgg ttcgtcgatt tgaatcactt taataccggc
    29341 tttttccaaa tccaatactt cgtcatttaa ggccaaggcg atttgtttgc acacggtgct
    29401 gcgcggaata tcgttgcgca caaatgacca ttgcagaatc gttaccggac cggtcaacat
    29461 gcctttcatc gggcgtttgg tcagggtttg ggcgtaggtt gaccaagcca cagtcatcgc
    29521 gttcgggcgg ctaacatcgc cgtaaatcac cggtggtttc acgcagcgtg agccatagct
    29581 ttgtacccag ccgtattggg taaaggcaaa gccttccagc aattcgccga aatattccac
    29641 catgtcgtta cgctcggctt cgccgtgtac caacacatcc aaattgagtt tttcttgctc
    29701 ttcaatcacc aaggcgattt cttttttcat cgcggcttcg taatcagcgt cagacaattc
    29761 gccttttttg aacgcggcac gcgcttggcg gatttcagtg gtttgcggga acgaaccgat
    29821 attggtggtc ggcagcaacg gcaggttcag ccatgcttgt tgcgctttaa tgcgctcggc
    29881 aaacggtgat ttgcgttggt cggcatctgc cggtaaatca gccaagcgtt tggccacttc
    29941 ggcgcggtgg atttcggtgc tgttggcgcg tgaatcagca gcggcttggc tgtcggccaa
    30001 ttcggcggca acggcttcgc ggccttggtt caatgcagtt ttcaacacgc gcaattcttg
    30061 cgttttctgc aaggtgaaag ccaaccatga gaacaaggca ggtttgttga ctttcagctt
    30121 ttcttctacc gacaaatcgt atggcgtgtg cagcagcgag catgagctgg aaatccacaa
    30181 gcggtcgccc aatttggctt tcaacggctc aagcgtggct aatacgttgt ttaaattggc
    30241 gcgccaaata ttacggccat caatcacacc ggcagacaac actttgccgt aatcagcaaa
    30301 tgcgtccaat tgctcgggcg cgcgcaccaa atcgatgtgc aaaccggcca caggcaaggc
    30361 tttcagacga cctgcgtgtg cggcaaccga accgaaataa gtgctcaaca agattttggc
    30421 attcacttgg ctcaaagtgg cgtaaacgtc ttggtacgca tccaaccacg cttgcggcag
    30481 gtcaaccgcc aatgctggct catcgatttg aatccattcg gcaccggcag cggccaattc
    30541 ggtcaggatt tcaacataaa ccggcaataa ttttggcaac aggctcaagc ggtcaaattc
    30601 gacagcgcct ttttctttac caacccacag gaaggtcaac ggaccaacaa cggtcggttt
    30661 ggctttcagg cccaaagctt gggcttcttg cagctgttga acgtagtgtt tggcgttcgc
    30721 tttgaattcg gtatcggcgt ggaattcagg caccaagtag tggtagttgg tgtcgaacca
    30781 tttggtcatt tcgatagcga attggtcttt attgccgcgt gccagttgga agaattgttc
    30841 caaagacagg ttttggctgt cgaagccgaa acgggcaggg atggcaccgg tagcgacttg
    30901 caggtcgagg atgtggtcgt agaaagtgaa atcgcctaca gcaacgtaat cagcgttggc
    30961 agcggcctgg tgtttccagt ttttctcgcg caagtcttta gcaacagcca gcaattcttg
    31021 ctcgctgatt tctttgcgcc agtatttttc ttgtgcgaat ttcaattcgc ggaaggcgcc
    31081 gacacgcggg aagcctgaga aatgtaatgt tgtcatgttg aactctccag ttggattttg
    31141 taaggaccgg ttcaatccga atcggccggt attgtaaatc agaatggctc ggatggggcg
    31201 gtgttttgct ttttagacac cttagatgcc gtctgaaact gtttgatacg gctttaaggg
    31261 cgcacgccta aaatatggca aatggcgtaa gtcagctcgc tgcggttaag cgtatagaag
    31321 tggaaatctt tcacgccttc gcgggacagg actttgacca tatcgatggc gatacttgcc
    31381 gccaccagat tgcgcgtacc ttggtcgtcg tccaaacctt catacatttg cgacagccag
    31441 cttgggattt tgacgttggt tacttgagcc attttaccga gctgcttgaa gttggtaaca
    31501 ggcaaaatac cggggacgat ttccacatcg atgcccaaca tcacgcagcg gtcgcggaag
    31561 cgcaggtagc gttccacatc gaagaagaat tgggtgatga cgtggttcgc gcccgcatcg
    31621 attttgcgtt tcaaattaat caggtcggct tgtgcggatt tcgcttcggg atgcacttcg
    31681 ggatatgctg ctacagaaat gtcgaagtcg gcgacggagc gtaatagctt aaccaagtct
    31741 tcggcataaa acggtttttt ctcgtaaccg ggcggctcgt cgccgcgcag ggcgacaatg
    31801 cggcggatgc cgctgtccca ataatctttg gcaatttggc gcaattcgtc gggagaagcg
    31861 tcgatgccgg tcaggtgcgg cgcggcttcc aagccggttt cttgcttaat gcgtttgacg
    31921 atgccgtgcg tgcggtcgcg ttcgccggaa tttgcgccgt aggttacgga tacgaacttg
    31981 ggatgcaggg tttgcagacg gtggatggaa tcccacagca tcgtttccat ttgttcgttt
    32041 ttcggcggga aaaattcaaa cgaaacgttg atgtcgcctt tcaaatcgga aaggctgttg
    32101 tttaacgcat tgatttcttt tgcgtaattc atggctgcgt accttttatt atcttttata
    32161 tgatatttca ggcaacataa taaaattgca ccgaatatga gtcaatttca aaattttcag
    32221 gtttatcatg aaaatttcta atatattaca aaaatagtta aatttccttg tacaagcctt
    32281 ctaaatttat gataaagttt gttatgttat aacaatccaa ctaaggcgat atatgaaacc
    32341 gaatattcat cctgacaatt atcgaaccgt cctttttttc gacagcagcg ccaatgaagg
    32401 ctggctgatt cgttcttgcg ccgaaacaca cggaaaaacg atggtttgga cagacggcaa
    32461 agaatatcca ctgttttcgc tggatacttc ttccgcatcc catcccgtct ataccggcaa
    32521 gcaacgcaac gtcaacaccg aaggccgtgc cagcaaattc aaccaacgtt tccaatccgt
    32581 catgtcatcg tttagaaagg acaaataatg caggttctgt cttcactcaa aaccgccaaa
    32641 caacgccacc gcgactgtca gattgtcaga cgcaaaggca aagtttacgt catctgcaaa
    32701 agcaatccgc gttttaaagc gcgccaacgc tgaccccctg cccttcctgc ttcggttgta
    32761 aatcccctcc taccgcaagc ccttttcatc aaatatgccg tctgaaacag aaaaatgcgt
    32821 ttcaggcggc attttttccg tcaatacgaa tccggaatgt gtttgaaata cagcataaac
    32881 agataccaaa acagcatcgc ggcggctgca ccgagcagta cccatgcgct gatttcggcg
    32941 acttcgcgca gggcgcgttt ttgcctgcgg ctgaaaaggc ggttgataat caacgcggca
    33001 agtgcgaaaa agaaaaaaat acggaaaagt ctgcctatca tgtcgggaag cggtggaggt
    33061 cgtctgaaaa agtatgattc gtgtgcggcg gatgagggtc aaacgcgtcc ggctgcttgt
    33121 ccgaccgtgt caaaaaatca tgccgtttgc gatataataa cgcttttcgc cgcatttgtt
    33181 ggaacaggat atgagtcaaa atgcctcggt gggcattgta acgccccaaa aaattccgtt
    33241 tgaaatgccg ctggttttgg aaaacggtaa aactttgccg cgtttcgatc tgatgattga
    33301 aacctacggc gagctgaatg ccgaaaaaaa taatgcggtt ttaatctgtc atgcgctgtc
    33361 aggcaaccat catgttgcgg gcaggcattc ggcggaggat aaatatacgg gctggtggga
    33421 caatatggta ggacccggca aaccgattga tacggaacgt tttttcgtgg tcggtttgaa
    33481 caatctgggc ggctgtgacg gcagcagcgg acctttgtcg atcaatcctg aaacgggtag
    33541 ggaatacggc gcggattttc cggtggttac ggtgaaggac tgggtaaaat cacaagccgc
    33601 gcttgccgac tatctcggca tcgggcaatg ggcggcggtt gtcggcggca gtttgggcgg
    33661 tatgcaggct ttgcagtggg cgatttccta tcccgaacgc gtgcgccatg ccttagtgat
    33721 tgcgtctgcg ccgaaactgt ccacgcaaaa tatcgcgttc aacgatgtgg cgcgtcaggc
    33781 gattttgacc gaccccgatt tcaacgaagg acattaccgc agccataata ccgttcctgc
    33841 acggggcttg cggattgccc gcatgatggg gcacattacc tatcttgccg aagacggttt
    33901 gggcaaaaaa ttcggacgcg atttgcgttc caacggctat caatacggtt atggcgttga
    33961 atttgaagta gaatcctatc tgcgctatca gggcgacaaa ttcgtcgggc ggtttgatgc
    34021 caacacctat ttgctgatga ccaaagcttt ggactatttc gatccggcgg cggatttcgg
    34081 caacagcctg acccgcgccg tgcaggatgt gcaggcaaaa ttctttgtcg ccagcttcag
    34141 caccgattgg cgtttcgcgc ccgaacgttc gcacgaactg gtcaaggccc tgattgccgc
    34201 ccaaaaatcc gtgcagtata tcgaagtcaa atccgcacac gggcacgatg cctttttaat
    34261 ggaagacgaa gcctatatgc gtgcggtcgc cgcctatatg aacaacgttt ataaggaatg
    34321 tcagcaatga acctgcgcga tgatttgcaa ctgatttacg accggatacc cgaaggcagc
    34381 cgcgtcttgg acttgggctg cggcgacggc gaactgcttg ccgcattggt cgaacacaaa
    34441 aaatgcagcg gctacggcat cgaaatcgac accgacagcg tgattgccgc gatgtcgcgc
    34501 ggcgtgaatg ttatccaagc cgatttggaa gaaggtttaa ccgcattcaa cgatcaaagt
    34561 tttgatgtga ttgtgttgag ccaaaccatc caagcgatgc agaataccga aaaaatcctg
    34621 cgctgcctga tgcgcgttgc caaacaggcg attgtcagct tcccgaattt cggctactgg
    34681 cgcaaccgcg tccaaatcgc actcggcgga catatgccgg tttccgaacg tatgccctac
    34741 cattggtacg acacgcccaa tatccattgg tgcacgctca aagactttga tttgttgtgc
    34801 gccaaaaaca acatccgcgt cctcgaacgc gccgtgatga cgggcaacag acaggtcaaa
    34861 catttcccca accttttggg cagccttgcg ttttaccgcg tcggctaaga caggttgaga
    34921 accttgtcgg caataacagg ttcttcggtt ctattaacaa caggccgtct gaaaaatgaa
    34981 acggtaggtt ttcagacggc ctctttaata ctggaaacaa tagaatgaaa aacaaaacct
    35041 catcacttct cttatggctt gccgcaatga tgctgaccgc gtgttccccg agcaaagaag
    35101 ataaaacgaa agaaaacggc gcatccgccg cctcgtccac ggcatccgcc gcttcgtctt
    35161 ccgcgcccca aaccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag
    35221 aaagcgcgcc gccgtcaaat tgcaccgacc tgcaccccgc caccggcatt gacgatctca
    35281 tgcagcaaat cgccgaacac atcgactcgg actgtctgtt tgccctttcc catcacgaac
    35341 tggaaacccg tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc
    35401 ccgacatccg ccctgaagat cccgactacc atcagaaaat catactggca attgaagact
    35461 tgcgttacgg aaagcgcacg atcagccggc aggcacaaga tgccttgatg gaacaggaac
    35521 gccgcctccg agaagcgacg ctgttgctga tacagggcag tcaagaaacc cgcggacaag
    35581 gcgaggagcc gaaacgcacg cgttattttg aagtttcggc aacccctgcc tattcgagcc
    35641 ggcacaacaa cggacttggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga
    35701 aaatacacgg agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca
    35761 atcccgacaa accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg
    35821 tcgtttacga aaaaaacatc tacttcaatc caaacttggg gcgaagataa aaaaatgccg
    35881 tctgaaacgt tttcagacgg catttttcaa tgcaacaaat caagcgcgtt tgcggaattc
    35941 gccggtgcgg gtatcgattt cgattttgtc gccgttttcg atgtaagaca tcacttggat
    36001 ttcggtgccc ccgaccaggc gcgcggtttt catcactttg cctgaagtat cgcctttgac
    36061 ggcaggctcg gtatactcga cttcgcgtac gatgatggtg ggcagttcta cggagatggg
    36121 gttgccttcg tagaaggtaa cttcgcattg gtcttccatg ccgtcaacga tgaatttcaa
    36181 cgcgtcgccg atgttgtcgg cttcgatttc gtattggttg aattcttcgt ccataaagac
    36241 gtacatcgga tcggcaaagt agctgtacgt acagtttttg cgggacagga tgaccacgtc
    36301 gaatttgtcg tcggctttgt aaatggtttc ggaagccgcg ccggtcagca ggtttttcag
    36361 tttcatgctg actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac
    36421 cataggatcg ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat
    36481 tgtgattttc caatcaaatc ggttgtcgta aaacgcacta ttctagcgta ttttttgatg
    36541 ctttgaaaca aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct
    36601 ccaatcctcc gcgccttgcc gccagccgtt ttgatgttgt tgcaggattt gccaacattc
    36661 gaggcgttgc gtggcggata aagcccctcc gccgttgagg tcgtccgaaa gacggcggtg
    36721 tgccgacacg gtttcgggcg tgtagaaacc gtgtgcctta tcccaaaagg cgtggagttt
    36781 gtcgagatgg acattctcgt cttgcgggta gatgtgccaa aagaagggtt tgccggcaag
    36841 ctgggcgcgc acgaaactgt cttcgccgcg tatcacggcg cagtcggcga ggtgcagcag
    36901 tttgtcgaag tcctgttgcg gcacgaaagg gattttgacg aggcggacgg atgccgtctg
    36961 aaaaacatcg ccgtcgtttt gcagggcatc ttgcggaata acgccgcttt gtttgaggct
    37021 gtcgatgatt tgcgtccccg ccagcaacag tgtcatcggg ctgcctgcct gtcgccacat
    37081 ttccagccac tttgcccaaa catcgctccg atagccgaaa agcagccatt cgggggcgtt
    37141 tttttcggga agcatcagcc gctgacgcag ggcttcgcta tcgaaacgga cggcttcgca
    37201 gtaatcgcgt tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata
    37261 tttttgaaca ccttcctgcg gcgaaggcat caggtgcagc ctttcattgc tttcctccgc
    37321 gctcaaatat tcccaattca gccaaagcgg cttgtgtcgg cggataatgt gcagcacatt
    37381 ttcgggcagg tcgcaggcaa aagtttcgat gacgacatcg ggaacaggcg cggtatcaat
    37441 atctgccgca tcggaatgcc aagtgcggac atgaatatcc tgatgaacgc agggaacatc
    37501 gggcaaatca gggcaaagcg cacgcaaggc ggacacatcg tccgtccaca aatgcacctg
    37561 ccaaccgagt tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa
    37621 attgtcgatg accttgcaaa aaatccaaca gacaaaagga ggagtattca tagaaataac
    37681 ggaaacgggc taaaacgcgc cgaaatagtg gaacaaatac acaatcagca aaataatcgg
    37741 aataaacagc aacatagttt gacgtttcaa atcaaactct tgcttcaaat gatgcttgat
    37801 ggtgtcgaaa aggacgaatt tgaacaaaaa cgtcgcttcg caggcaagga tgatgccgtt
    37861 aatcagatgc tggggtttgt cttcgggcgg attggcggcg aaaacggcaa gcgtcgccaa
    37921 gggtatcagg cagacgacaa gccagcggac aagcaggttt cgggacattg caaggctttc
    37981 aaaaaagcaa aacgccatta tcgccccaag cgcacgcctt gtctaacgga tgcgccggag
    38041 atgccgtctg aagccttgcc gcaggaaaac accgcaccgc cgaaccttta aatacgcccg
    38101 agacgggcgt tccccgaaaa atcggacgct gccgccgatg cctgaggaca aggcggcagc
    38161 aggcacgggc atattgaaaa cccggcgcgt cggtttgaaa ttttatagtg gattaacaaa
    38221 aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggaaccg attcacttgg
    38281 tgcttcagca ccttagagaa ttattctctt tgagctaagg cgaggcaaca ccgtactggt
    38341 ttttgttaat ccactatata aaggcatacg gtaaacacgc ccccttgccg tcggcgtgct
    38401 tccgcaaacc aaaatataac gtcggcacgc tttccgctat aatccgtatt tttccaaacc
    38461 tgaaacgtta tgccgacccc gaaagccttt gccctgctcg ggccgaccgc cggaggcaaa
    38521 accgcgctcg ccctcaaaat tgccgaaacc ctgcctgtcg aaatcatcag cctcgattcc
    38581 gcgctggttt accgcgatat ggacatcggc acggcgaaac cgtccgcctc cgaacgcgcc
    38641 tttgtcccgc accaccttat cgacatcatt acgcctgttc agacatacag tgccgcccgt
    38701 tttgtcgaag actgcacgcg tctgattggc gaaatcacgg cgcgtgggaa atatccgctg
    38761 attgtcggcg gcacgatgat gtatttccgc gccctgaccc aaggtttgaa cgatttgccc
    38821 gaagccgatg cctgcctgcg tgccgacttg gacgaacaaa aacaaatgta cggcttggat
    38881 ttcctctacc gcaccctgca acaggtcgat cccgaaacag catgccgtct gaaaccgaac
    38941 gacagccagc gtatcggacg cgctttggaa gtttattatc tgaccggcaa accgatgagc
    39001 gaacacctcg gcagacaaag cccccatacg ctccccttcg atttgcatac cgccgccctg
    39061 attcccgaaa accgcgcccg cctgcatgaa aacatcgccc tgcgtttcca cctgatgctt
    39121 gaacagggct ttatcggcga agtagaaaac ctgcgccgcc gctatcccgg cctgaccgcc
    39181 gactcccccg ccatccgctg cgtcggctac cgtcaggcat gggaatatct ggacggaaaa
    39241 accgatttcc cggcatttgt cgagaaaggc attgccgcca cccgccaact tgccaaacgc
    39301 caactgactt ggttgcgcaa aacaccttta gactgcgttg ccgacccgtt ttcagacggc
    39361 acttcatgca cacgcctgat tgaggcggca aaacggtttt tcggcgcata aaccatgctg
    39421 accacaccgc cgctcgcccc caaaaccgtc gccgccctgc acaggctcgg tatccgtacg
    39481 ctcgaagaac tgcggcaaaa cggttcggtc aaagcctttc tgctgctcaa agcatcgggt
    39541 ttgaccctca cgaaaagcac gctgtggcag cttgagtcgc tgctgaacgg cacactcccg
    39601 caagaaatca cgcaacacga caaaacgcgc ctttttgccg aattgaaaaa ccatccgccc
    39661 gtcgccgcct ttccgccgca ggaagaaatg gaacacttca tgcgcgaagc actgcgccaa
    39721 gcagaacaat ccgccgctga cggcgaaata cccgtcgggg cagtcatcgt ttcagacggc
    39781 aaaatcatcg catcggcaca caacacctgc attgccgact gcaatgtcag ccggcacgcc
    39841 gaaatcaacg ccttgtcaca ggcaggcagc gaaatgcaaa actaccgcct tgacggatgc
    39901 gacatatata tcaccctcga accctgcgcc atgtgcgcgt ccgcactgat acaggcacga
    39961 atcaggcgcg tgatctacgg ggcggcagaa cccaaaaccg gcgcggcagg cagcatcgtc
    40021 aacctgtttg ccgacaaacg cctcaatacg cacaccgcca tacggggcgg aatcctgcaa
    40081 gaagaatgcc gcgccgtgtt aagccgtttt tttcaaaaca aaagaaaagg ttgaacctat
    40141 gaccgtactg accgtcatcc tcgccctcgc cctgatagcc gtcggcacgg cgggcatcgt
    40201 ttaccccgcc ctgcccggat tggcattgat gtttgccgga acatggctgc ttgcctatgc
    40261 cggcggctac caaatctacg gcgcgggcgt tttgtggacg gtcggactca tcagccttgc
    40321 cggcatactg gcggactatg tggcaggcat atgggggaca aaatataccg gagcgggcaa
    40381 gctcgccgtt cgcggcgcat tggccggcag catcatcggc atatttttct cccttcccgg
    40441 actaatactc ggtcccttta tcggcgcggc ggcaggcgaa ctgatcgaac ggcgcaatat
    40501 gcttcaggca ggtaaagcgg gcttgggtac gctgttgggg cttgtcgtcg gcacggcgtt
    40561 caaaatcggc tgcgccgtat ccatcttgtt tatcctgttg gtgaaataca tcgcctacct
    40621 gttttaaccc tttcagacgg cataccggca aattgctata atgctgtctg aacccttttt
    40681 acaaagcacc atcatggcaa accaaagacc catctacggc ttccacgccg tcaacgcccg
    40741 attgtggcaa aaccccaaat ccatcgtcga actctacatc caagaaggca aatcagacgc
    40801 acgcacgcgc gaagtgttgg aaaaggcggc aaacgaaaac atccgcgtat attttgtcga
    40861 tgccgaccgc ctcaacgcca tcagcaaagg cgcgcgccat cagggcgtgg tcggatttat
    40921 cgatgcctcc aaaaaccacg tccacctcga agacgtattg gaaaacctca gcgaaccgcc
    40981 gctgctgctg atactcgacg gcatcaccga tccgcacaac ctcggcgcgt gcctgcgtac
    41041 cgccgacgca atgggcgtac acgccgtcat cgcaccgaaa gacaaaagcg cggggctgaa
    41101 cgccaccgtc agcaaagtcg cctgcggtgc ggcggaaacc gtcccctata tcaccgtaac
    41161 caacctcgcc cgcaccctgc gcgaattgaa agaatacggc atttggatta tcggcaccga
    41221 tatgagcggc gagtccgacc tttaccattg caatctgccc gacagcgcgg cgtgggtaat
    41281 gggcaacgaa ggcgacggta tgcgccgcct cacgcgcgaa cattgcgaca tgctggtgtc
    41341 catacccatg ttcggcacag tcgaaagcat gaacgtctcc gtgtctgccg gaatggtatt
    41401 aagtgaaaca cgccgacaac gcgtattaaa aaacgaaaaa gtataagcct caaaaacaat
    41461 gccgtctgaa acagggattc tgtttcagac ggcattcatt ttcaaaatca gtgttccaag
    41521 tggtcggtat caaacttgac cgccgcctcg gttttatctt cgggcaagac caaattcagc
    41581 aagactgccg taatgccgcc ggcggaaata gagttttgga acaagacggg caggttttta
    41641 aacacttccg gctcaaacgc gacacccaag cccaaaccga ccgacgttgc cgcaatcacc
    41701 gcttcgcgcc tgcggatgcc gtgactgacc aaaatccgca cgcccgcaat cgcaattaag
    41761 ccgaacatca aaaccatcgc gccgcccaac accggactcg gaatcgtcgt aaacgcgcga
    41821 ccgacaacgg ggaacagacc caacagcacc aaaatcacgg caatatattt gcccacatga
    41881 cgcgaagcca cgccggtcat ctgaatcacg ccgttgtttt gcgcaaacgt cgtcagcggc
    41941 agcgaaccca aagccgtcgc aatcaccgac accaagccgt cagccaacac gccgccgcgc
    42001 aggcgtttgg tgtattcctc gccttcaatc ggctgatcgg acaccattgc cgttgcggtc
    42061 aaatcgccga ccgcctcaaa cacgctcaac aagaaaatcg cgcccgccac aataaacgcg
    42121 tgccagtcaa aagcaaaacc atatttaaac ggtacgggca gcgtaaccag cggcaggttt
    42181 tgcagcgcgg aaaaatccac cttgcccaaa aacagcgcga cgatatagcc ggcaatcagc
    42241 ccgaccgcaa tgccgctcat gcgcaacagc gggtttttca tgcagttgaa caccaacaca
    42301 atcagcaaca ccagcgatgc cagccccaag ttttccatcg agccgaacgt gccgtccgcc
    42361 ttcgcgccga agccgccgcc gaaatcggta atgccgacgt gtaccaaact caaaccaatg
    42421 agcatcacga ccacgccgct gaccgtcggc gtaatcacct ttttcaaata cggcagaagc
    42481 cacgccgaga aacacaccaa aaacgcgccg acaaacgata cgcccaagag cgtcgaaatc
    42541 atcgcatcct tagtcaaacc gccctctttc atccccgcgc cgagcgcaat catgacagta
    42601 acgaacgaga aattcaccga ctggatggac agcatccccg aaccgaccgg cccgaagcgg
    42661 ttgacctgca aataagtgcc gacacccgac gcaaccatcg ccatcgacac gagatacgcc
    42721 gtcatctcca ccggcaattc cagcgcgccg cccacaatca gcgcgggcgt aatcatcggc
    42781 acaaaaatcg ccaaaagatg ggtaaccgcg ctcaagagcg cattaccgaa cggcggccta
    42841 tcctccaaac cgtacaccaa atcaggcgca tccgcacctt tgcccaactg accgctcatt
    42901 gtatttcctt attttaaagg ttgaaaaatc caagcgcgga ttgtagcgaa tctgccgcac
    42961 ggcttcaatt tgcatttcag acggcatccg tctggcccac acgtttacca cattttatag
    43021 tggattaaca aaaatcagga caaggcggcg agccgcagac agtacagata gtacggaacc
    43081 gattcacttg gtgcttaagc accttagaga atcgttctct ttgagctaag gcgaggcaac
    43141 accgtactgg tttttgttaa tccactataa atccgacggc accggcataa accggcgaat
    43201 ataataaaat gatttcaaac aggaaaaatc aatttccacc atacctttcg gcgcaaacgc
    43261 cccacatccg caacccgccg gatttcacac ctgcggtaaa gtaggctaca atcggaagca
    43321 ttaccgcccc ctacaccaca actgataaaa ggttctgtat gttacaaggt agcctggttg
    43381 ccctgattac cccgatgaat caagacggca gcatccatta cgaacaactc cgcgacttaa
    43441 tcgactggca cattgaaaac ggcacggacg gcatcgttgc cgtcggcacg acaggcgaat
    43501 ccgccaccct ctccgtcgaa gaacacaccg ccgtcatcga agccgtcgtc aaacacgttg
    43561 ccaaacgcgt ccccgtcatc gccggcacag gggcaaacaa caccgtcgaa gccatcgccc
    43621 tttcccaagc cgccgaaaaa gccggggcgg actacaccct ctccgtcgtc ccctattaca
    43681 acaaaccttc gcaagaaggc atgtaccgcc atttcaaagc cgttgccgaa gccgccgcga
    43741 ttccgatgat actctacaac gttcccggca gaaccgtcgt cagcatgaac aacgaaacca
    43801 tcctgcgcct tgccgaaatc cccaacatcg tcggcgtgaa agaagccagc ggcaacatcg
    43861 gcagcaacat cgaactcatc aaccgcgcgc ccgaaggctt cgtcgtcctg tccggcgacg
    43921 accacaccgc cctaccattc atgctctgcg gcggacacgg cgttattacc gtagccgcca
    43981 acgccgcacc caagctcttt gccgatatgt gccgcgccgc gcttcaaggc gatatcgccc
    44041 tcgcacgcga gctcaacaac cggctgattc cgatttacga caccatgttc tgcgaaccca
    44101 gccccgccgc accgaaatgg gcggtatccg ccctggtcag atgcgaaccg cacgtccgcc
    44161 tgccgctcgt tccgctgacg gaaggcggac aggcaaaagt ccgcgccgcc ctgaaagcct
    44221 caggacaact ttaaatatag ccatgccgtc cgaaccgttc ggacggcata acgcaacaaa
    44281 cactttaata tccatcacac aggatgacac gatgacccat atcaaacccg tcattgccgc
    44341 gctcgcactc atcgggcttg ccgcctgctc cggcagcaaa accgaacagc ccaagctcga
    44401 ctaccaaagc cggtcgcacc gcctgatcaa acttgaagtc ccacctgatt tgaacaaccc
    44461 cgaccaaggc aacctctacc gcctgcctgc cggttcgggc gccgtccgcg ccagcgattt
    44521 ggaaaaacgc cgcacacccg ccgtccaaca gcctgccgat gccgaagtat tgaaaagcgt
    44581 caaaggtgtc cgcctcgagc gcgacggcag ccaacgctgg cttgtcgttg acggcaaatc
    44641 ccccgccgaa atctggccgc tcctgaaagc cttctggcag gaaaacggct tcgacatcaa
    44701 atccgaagaa cccgccatcg gacaaatgga aaccgagtgg gcggaaaacc gtgccaaaat
    44761 cccccaagac agcttgcgcc gcctattcga cacagtcggt ttgggcggca tctactccac
    44821 cggcgagcgc gacaaattca tcgtccgtat cgaacagggc aaaaacggcg tttccgacat
    44881 cttcttcgcc cacaaagcca tgaaagaagt gtacggcggc aaagacaaag acacgaccat
    44941 gtggcagcct tccgcttccg accccaacct tgaggccgct ttcctgacgc gctttatgca
    45001 atatttgggc gttgacggac agcaggcgga aaacgcatcg gcaaaaaaac ctacccttcc
    45061 cgccgccaac gaaatggcgc gtatcgaagg caaaagcctg attgtctttg gcgactacgg
    45121 cagaaactgg cggcgcaccg cgctcgccct cgaccgcatc gggctgaccg tcgtcggtca
    45181 aaacaccgaa cgccacgctt tcctggttca aaaaaccccg aacgaaagca atgcagttac
    45241 cgaacaaaaa cccggcctgt tcaaacgcct gctgggcaaa ggcaaagcgg agaaacctgc
    45301 cgaacagccg gaactgattg tctatgcaga acctgtcgcc aacggctcgc gcatcgtcct
    45361 gctcaacaaa gacggcagcg catatgccgg caaagacgca tccgcactgt tgggcaaact
    45421 ccattccgaa ctgcgttaaa ccgttcaaaa caaaagcagc ttccaaacag aagctgcttt
    45481 tttgtgcgga actttttaca acaaccgagg aaaaatatcc tcaaccgcct gaaccaacgc
    45541 atccgccaaa ggcggatcga acgcacgatg ccctgcctga accaccctca attccgcttc
    45601 cggaaaggct ttcgacagcg cccacgcact ctgcatcggc gtacacaaat cataccgccc
    45661 ctgtacgata acggtcggga tatgccgtat tttgccgata ttgttcaaaa tcgccctatc
    45721 gccctgcaac cagccgccgt tgacaaaata atggttttcc aaacgcgcga ttgccagcga
    45781 ggcataagca tcttcatcca cttcctccgg ctcgaaacgg atcagatagc tttcccaatc
    45841 cgcccaagcc ttcgcggcag acagacacac ttcttcatct tgatgaaaca gcaatccgtg
    45901 atacgcctca atcagccggt tccgccgatt ttcagcaatc ggcgcgacaa atttttgcca
    45961 ttgttccgga taaatccggc tcacaccgcc cgcctcgttc agccacaccg tttcagacgg
    46021 tctgcacaaa aatatcccgc gcaacaccaa tccctttacc cgttccggat gggtttgggc
    46081 ataagccagc gacaaagtgc tgccccacga accgccgaac accagccatt tcccgatacc
    46141 cagcatttca cggacttttt caatatccgc caccaaatcc caagtcgtat tgtcttccgc
    46201 acaagcatac gggcgcgaac gtccgcaacc gcgctggtcg atgatgacga tgcggaacac
    46261 atcgggattg aaaaaacccc gacattcagg cgaagccccc gcgcccggcc cgccatgtaa
    46321 aaaaataacc ggcacaccgt caggattacc cgactcttcc caataaattt gatgaatttc
    46381 agatacctgc aagtatccgc tatgaaaagg ctgttttatc tcatacatca tcttctccca
    46441 tattgcttct gacaatccga acatcccgac acgcgacatc ccgcacaaaa cacaagccgc
    46501 cccgaaaggc aaatgccgaa aaatcgcgat atagtggatt aacaaaaatc aggacaaggc
    46561 gacgaagccg cagacagtac aaataatacg gaaccgattc actcgtgctt cagcacctta
    46621 gagaatcatt ctctttgagc taaggcgagg caacgccgta c
//
LOCUS       DAWFIT010000004        42792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFIT010000004 DAWFIT010000000
VERSION     DAWFIT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.004"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(251..880)
                     /locus_tag="WGQ85_000197"
     CDS             complement(251..880)
                     /locus_tag="WGQ85_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0749711.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVEPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            1179..2513
                     /locus_tag="WGQ85_000198"
     CDS             1179..2513
                     /locus_tag="WGQ85_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0749712.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAATSLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            2506..4167
                     /gene="pqiB"
                     /locus_tag="WGQ85_000199"
     CDS             2506..4167
                     /gene="pqiB"
                     /locus_tag="WGQ85_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0749713.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            4167..4685
                     /locus_tag="WGQ85_000200"
     CDS             4167..4685
                     /locus_tag="WGQ85_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0749714.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            4689..5240
                     /locus_tag="WGQ85_000201"
     CDS             4689..5240
                     /locus_tag="WGQ85_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0749715.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            5230..5871
                     /locus_tag="WGQ85_000202"
     CDS             5230..5871
                     /locus_tag="WGQ85_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0749716.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSSVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(5864..6094)
                     /locus_tag="WGQ85_000203"
     CDS             complement(5864..6094)
                     /locus_tag="WGQ85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0749717.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADHLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            6042..6401
                     /locus_tag="WGQ85_000204"
     CDS             6042..6401
                     /locus_tag="WGQ85_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749718.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            6432..9284
                     /gene="gcvP"
                     /locus_tag="WGQ85_000205"
     CDS             6432..9284
                     /gene="gcvP"
                     /locus_tag="WGQ85_000205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0749719.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(9749..10588)
                     /locus_tag="WGQ85_000206"
     CDS             complement(9749..10588)
                     /locus_tag="WGQ85_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0749720.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            10873..10948
                     /locus_tag="WGQ85_000207"
     tRNA            10873..10948
                     /locus_tag="WGQ85_000207"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10906..10908,aa:Arg,seq:ccg)
     gene            complement(11013..12206)
                     /locus_tag="WGQ85_000208"
     CDS             complement(11013..12206)
                     /locus_tag="WGQ85_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0749721.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(12227..13315)
                     /gene="trmA"
                     /locus_tag="WGQ85_000209"
     CDS             complement(12227..13315)
                     /gene="trmA"
                     /locus_tag="WGQ85_000209"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0749722.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDTSVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGGFTQPNAAVCQKMLEWACGAAEGLGGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFARLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     VVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            13462..14562
                     /gene="aroC"
                     /locus_tag="WGQ85_000210"
     CDS             13462..14562
                     /gene="aroC"
                     /locus_tag="WGQ85_000210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0749723.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            14654..15058
                     /locus_tag="WGQ85_000211"
     CDS             14654..15058
                     /locus_tag="WGQ85_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0749724.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(15132..17117)
                     /gene="parE"
                     /locus_tag="WGQ85_000212"
     CDS             complement(15132..17117)
                     /gene="parE"
                     /locus_tag="WGQ85_000212"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0749725.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGIEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(17184..17708)
                     /locus_tag="WGQ85_000213"
     CDS             complement(17184..17708)
                     /locus_tag="WGQ85_000213"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0749726.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            17873..19168
                     /gene="serS"
                     /locus_tag="WGQ85_000214"
     CDS             17873..19168
                     /gene="serS"
                     /locus_tag="WGQ85_000214"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0749727.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSILDEGGLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(19246..20244)
                     /locus_tag="WGQ85_000215"
     CDS             complement(19246..20244)
                     /locus_tag="WGQ85_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0749728.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(20369..21445)
                     /gene="prfA"
                     /locus_tag="WGQ85_000216"
     CDS             complement(20369..21445)
                     /gene="prfA"
                     /locus_tag="WGQ85_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0749729.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(21535..22305)
                     /locus_tag="WGQ85_000217"
     CDS             complement(21535..22305)
                     /locus_tag="WGQ85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0749730.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(22420..23409)
                     /locus_tag="WGQ85_000218"
     CDS             complement(22420..23409)
                     /locus_tag="WGQ85_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0749731.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELVRLKL"
     gene            complement(23476..24159)
                     /locus_tag="WGQ85_000219"
     CDS             complement(23476..24159)
                     /locus_tag="WGQ85_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0749732.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(24348..25682)
                     /gene="glmM"
                     /locus_tag="WGQ85_000220"
     CDS             complement(24348..25682)
                     /gene="glmM"
                     /locus_tag="WGQ85_000220"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0749733.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(25812..26669)
                     /gene="folP"
                     /locus_tag="WGQ85_000221"
     CDS             complement(25812..26669)
                     /gene="folP"
                     /locus_tag="WGQ85_000221"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0749734.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(26809..27702)
                     /locus_tag="WGQ85_000222"
     CDS             complement(26809..27702)
                     /locus_tag="WGQ85_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0749735.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            27793..29907
                     /locus_tag="WGQ85_000223"
     CDS             27793..29907
                     /locus_tag="WGQ85_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0749736.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(29972..31450)
                     /gene="ubiD"
                     /locus_tag="WGQ85_000224"
     CDS             complement(29972..31450)
                     /gene="ubiD"
                     /locus_tag="WGQ85_000224"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0749737.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(31470..31985)
                     /locus_tag="WGQ85_000225"
     CDS             complement(31470..31985)
                     /locus_tag="WGQ85_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0749738.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNWLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(31992..32522)
                     /locus_tag="WGQ85_000226"
     CDS             complement(31992..32522)
                     /locus_tag="WGQ85_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749739.1"
                     /translation="MVRQGEATLYWFLLIHYHYGREAGWFVWLAAAMCGLWLLRALTA
                     KLPQQRYAALFITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLA
                     RLQQPDLPEKAVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMG
                     MPFAGEWLYRKLVLKV"
     gene            complement(32725..33294)
                     /locus_tag="WGQ85_000227"
                     /pseudo
     CDS             complement(32725..33294)
                     /locus_tag="WGQ85_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            33334..33944
                     /locus_tag="WGQ85_000228"
                     /pseudo
     CDS             33334..33944
                     /locus_tag="WGQ85_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            33889..35025
                     /locus_tag="WGQ85_000229"
     CDS             33889..35025
                     /locus_tag="WGQ85_000229"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ0749740.1"
                     /translation="MDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVR
                     MMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     PSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLW
                     DGQNDPDLPAINLTGSGSRWETEKRIAASSSFAFGGSNCVLIIG"
     gene            35079..35154
                     /locus_tag="WGQ85_000230"
     tRNA            35079..35154
                     /locus_tag="WGQ85_000230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35112..35114,aa:Ile,seq:cat)
     gene            35229..35987
                     /locus_tag="WGQ85_000231"
     CDS             35229..35987
                     /locus_tag="WGQ85_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0749741.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            35988..37052
                     /locus_tag="WGQ85_000232"
     CDS             35988..37052
                     /locus_tag="WGQ85_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0749742.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            37286..38623
                     /locus_tag="WGQ85_000233"
     CDS             37286..38623
                     /locus_tag="WGQ85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0749743.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
     gene            complement(38858..39193)
                     /locus_tag="WGQ85_000234"
                     /pseudo
     CDS             complement(38858..39193)
                     /locus_tag="WGQ85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(39174..39455)
                     /locus_tag="WGQ85_000235"
     CDS             complement(39174..39455)
                     /locus_tag="WGQ85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEZ0749744.1"
                     /translation="MGRHFGRRRFLTAAAVAAAGAAVSFLPNPFAADDEKRNGDEKRS
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(39464..40258)
                     /locus_tag="WGQ85_000236"
     CDS             complement(39464..40258)
                     /locus_tag="WGQ85_000236"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0749745.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            40642..41976
                     /gene="gdhA"
                     /locus_tag="WGQ85_000237"
     CDS             40642..41976
                     /gene="gdhA"
                     /locus_tag="WGQ85_000237"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0749746.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            42118..42492
                     /locus_tag="WGQ85_000238"
     CDS             42118..42492
                     /locus_tag="WGQ85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749747.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            42536..>42792
                     /locus_tag="WGQ85_000239"
     CDS             42536..>42792
                     /locus_tag="WGQ85_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0749748.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT        10086 a        11864 c        10965 g         9877 t
ORIGIN      
        1 ttatttgacg gcgtttctga ggatttgact acaggtgtac cccctccccc tgaaatacag
       61 gcggaaaggg cgaggatgga tgccattgca agcaggcttg gtttgaaagg aatcgtcata
      121 attatctcct atggcatatt tttaataaaa aatatgataa ttatttatat ctgatggttt
      181 gaaaaatgcc gtcatacgcg gcagaaaata cggttccgct tcacgagccg aggcgaaacc
      241 gtaaaatata tcaatgcctg tgcggcatca taccctcggg ggcttcggca tcggctgcca
      301 agccgaaagt ttcgcgcaac acgactttgt agaatgcaaa ggcttcgcgc gcgccttgga
      361 tggcttccgc ttcggcttcg ggagtcaagt tcaaggcgtt cagatgctcg acgaaggcgc
      421 gccagtgttt accgcgaccg tcgggatggg gggcgaggtg gcgcgcgccg tgttcgccgt
      481 tgtaatcgag tttttgcgcg tgcttgaaca aaaacgccgc acccaaatta gaaccttcgg
      541 cgcaataaag ccagccgatt gctttgttgc cggtttcgtg tggcaattct ttgtcgaatt
      601 tgtaaggctc ttcgcccaag tctttcaagt cttgcgttac ggcatcgtat cgcgccatgt
      661 attccagctc gggaatggct ttgtttaatt cggcatcttt atagatgtgg tcgacggcct
      721 tgtggaaaac ggattggagt ttcaaaaatt taatgtagtt ttctttgctg acaaacggtt
      781 cgacagacat aacgagatta tccacgctgt cgtgaaccgc cgtggtatct gctttcaagc
      841 gtttggcaaa cgtcagggct tgattttcgg tttcgctcat agcttttcct ttgtcggtaa
      901 gggataagga tgcggtgcgg gcaaaatccg tacctgaccg catacaaaaa taataaacga
      961 gatttattat cacatatttt tggaaaaaat atcatttgcg tgatgttttt aagcaggtat
     1021 tttactattc tttacagaat cgggatttta tcaaatgggt tcggcagtcg gcggacagcc
     1081 gctcaaaaaa tatttttgcc ggacaccaag ggtttgttca tactgccgaa cctgccggtt
     1141 ttgcatcctg attgggtgcg ttgctttttt cctttataat gccgccactt atatttgcca
     1201 ctttcccgat gaagccgttt gccgaaaata tcccccacag ccttcgcggc aactgccgcg
     1261 acgaagccct gccgccgcat acggtagatt gcccggaatg cggctgccgc gcggatgtgc
     1321 cgcaattgga caggggggag gcggcgttct gcccgcgctg cggctataag ctcttcaggg
     1381 tgggcaggca tcctttttcc gccccgcccg cttatgcggc gacttcgctg attttgatgg
     1441 cgtttgctta cggtatgacg tatatcgagg tcgggatacc gggtgcggca tccgtccttt
     1501 cgctgcccga gatgatgcgc ctgatggtgt ttcaggatta tggttttttg gccgaagtga
     1561 tgtttgtgct gaccttcggc gcgccggttc tgtttctgct gctgtgcctg tatgtctatg
     1621 ccgcgctgat acggaaacag gcgtatcctg cgctgcgttt ggcaacgcgt gtgatggtgc
     1681 gcttgagaca ggcgatgatg gtggatgtgt tttttgtttc cactttggtg gcgtatatca
     1741 agctctcgtc tgtggcagag gttcgcttcg gatcggcgtt ttatctgatg ttcgcgctgt
     1801 cggttatgct gattcggact tcggtatcgg ttccccagca ttgggtgtat tttcaaatcg
     1861 ggcggctgac gggggataat gcggttcaga cggcatcaga aggcaaaacc tgttgcagcc
     1921 gctgcctgta tttccgcgac agtgccgaat ccccctgcgg cgtgtgcggt gcggaactgt
     1981 accgccgacg gccgaaaagt ctgagtattt cgtcggcgtt tctgacggcg gcggttattt
     2041 tgtattttcc tgccaatatc ctgccgatta tgatttcatc caatcctgcc gccacggagg
     2101 tcaataccat ccttaacggc atcgcttata tgtgggacga gggcgacagg ctgattgcgg
     2161 cggttatttt cagcgcgagt attttggtgc cggtactgaa gattgcggca atgtcggttt
     2221 tgattgcgtc cgcccgcttc gctttgccaa cgggcgcaaa gaaattgtcg cacctctacc
     2281 gcatcaccga agcagtcggc cgctggtcga tgattgatat ttttgtgatt attattttga
     2341 tgtgttcgtt ccacacttat gccgcgcgcg tcattccggg cagtgcggca gtctatttct
     2401 gcctggtcgt gattctgacg atgctgtccg cctattattt cgacccgcgc ctgctttggg
     2461 acaaacgcgc ttcagacggc attgctttca atgaaacgga aaaatatgac tgacaacagc
     2521 cctcctccaa acggacacgc ccaagcacgc gtccgcaaaa acaacacctt gctctccgcc
     2581 gtctggctgg tcccgctgat tgcgctgatt gccggcggct ggctttgggt taaggaaatc
     2641 cgcaacaggg ggcctgtggt tacgctcttg atggacagcg cggaaggcat tgaggtcaac
     2701 aatacggtca ttaaggtatt gagcatcgat gtcggacgcg ttacccgaat caaactgcgc
     2761 gacgaccaaa aaggcgtgga agtaaccgcc caactcaatg cggacgtatc cggcctcatc
     2821 cgcagcgata cccagttttg ggtggtcaag ccgcgtatcg accaaagcgg cgtaaccggt
     2881 ttgggtacgc tgctttcggg ttcgtacatc gcttttacac ccggcaaaag cgacgaggca
     2941 aaagacgtgt tccaagtgca ggacattccg cccgttaccg ccatcgggca aagcgggctg
     3001 cgcttgaatt tgattggtaa aaacgaccgc atcctcaacg tcaacagccc tgttttgtat
     3061 gaaaacttta tggtcgggca agtcgaaagc gcgcatttcg acccgtccga ccaaagcgtg
     3121 cattacacca tcttcatcca aagccccaac gacaaactga ttcattccgc cagccgtttc
     3181 tggctggaaa gcggcatcaa tatcgaaacc acaggcagcg gcatcaaact caattccgcc
     3241 cctctgcctg ccctgctgtc gggcgcgatt tcatttgatt cgccgaaaac caaaaacagt
     3301 aaaaacgtca aaagcgaaga cagcttcacg ctttacgaca gccgcagcga agtcgccaac
     3361 ctgcctgacg accgctcgct gtactacacc gcgtttttca aacaatccgt gcgcggcctg
     3421 accgtcggtt cgcccgtcga gtacaaaggg ctgaatgtcg gcgtggtttc cgatgtccct
     3481 tatttcgacc gcaacgacag cctgcatctg tttgaaaacg gttggattcc cgtacgcatc
     3541 cgcatcgagc cttcccgttt ggaaatcaat gccgacgagc aaagcaaaga acattggaaa
     3601 caacaatttc agacggcctt aaacaaaggc ctgaccgcca ccatctccag caacaacctg
     3661 ctgaccggca gcaaaatgat tgagttgaac gatcagcctt cgtcttcgcc caagctgcga
     3721 ccgcataccg tttatgcagg cgataccgtt atcgcgaccc agggcggcgg tttggacgat
     3781 ttgcaggtca aattggcgga tttgctggac aagttcgaca aactgccttt agataagacg
     3841 gttgccgaat tgaacggttc gcttgccgag ctcaaatcca cactcaaatc tgccaatgcc
     3901 gccctaagct ccatcgacaa actggtcggc aaaccgcaga cacaaaacat tccgaacgaa
     3961 ctgaaccaaa ccctgaaaga gttgcgcaca accctgcaag gcgtatcgcc tcaatcgcct
     4021 atctacggcg acgtacaaaa tacgctgcaa agtttggaca aaaccttaaa agacgttcaa
     4081 cccgtcatta acactttgaa agaaaaaccc aacgcgctga ttttcaacag cagcagcaaa
     4141 gaccctatcc cgaaaggaag ccgataatgc gcctcttccc catcgccgcc gccctgacgc
     4201 ttgccgcctg cggtactgtg caaagcacac aatatttcgt gttgcccgac agccgctaca
     4261 tccgccctgc aacgcaaggc ggcgaaaccg ccgtcgaagt ccgccttgcc gaaccgctca
     4321 aacgcggcgg actggtctat caaaccgacc cctaccgcct caacaccgca caaaaccacg
     4381 tttgggcgga caccttggac gatatgctcg aagcggcgtt gagcaatgca ttcaaccgtt
     4441 tggacagcac acgcatcttt gttcctgcct cacgcagcgg cagtaccgaa aaatggacgg
     4501 tctatattga cgcattccaa ggcagctaca cgggcaaaac cctcatcagc ggctacgccg
     4561 tcctacccga cggtacgaac agacccttcc atatcgaaac cgaacagcag ggtgacggct
     4621 acgccgcgat gaccgccgca ctcgaacagg gactgaaaca ggcggcgcaa cagatggtcg
     4681 agtaaaccgt gaactattgc gaatttgccg cctcacttcc cgaaaacacc gacaacccga
     4741 acaaacatta ccacgacacg caatacggtt ttccgattga ggacgacaat gaattgtttg
     4801 aacggctggt gttggaaatc aatcaggcag gattgagctg gacgctgatg ctgaagaagc
     4861 ggcaggcgtt tcagacagca tttgaagggt tcgacatcga tacggttgcc gcatttgacg
     4921 aagccgatat tgagcggctg cttgccgatg cgggcatcgt ccgcaaccgc ctgaaaatcg
     4981 atgccgccat tttcaatgca cggcaaatcc aagcgttgca acaagaatac ggctcgttca
     5041 agaactggct tgacgcgcac catccgcgaa gcaaagacga atgggtcaaa ctctttaaaa
     5101 aacatttcaa attcgtcggc ggcgaaatcg tcggcgaatt tctgatgagt accggctacc
     5161 tcaaaggcgc gcacgccgaa agctgtccgg tttaccgcga aaccctgaaa taccacccga
     5221 aatggctcga tgccgtctga aaaaccaatg aacagaagaa tcttcctcct cggcgcaggc
     5281 gcgttgctgc ttaccgcctg cggcagaaaa tccgcccgaa cccacgccaa aattcccgaa
     5341 ggaagctccg tacttgcctt gggcgattcg cttaccttcg gctacggcgc aaaccctggc
     5401 gaatcctacc ccgcgcaact gcaaaaactg acgggttgga atattgtcaa cggcggcgta
     5461 tcgggcgata catccgccca agccctgtcg cgcctgcccg cgctgttggc acgcaaaccc
     5521 aagcttgtgc ttgtcggcat aggcggcaac gactttctgc gcaaagttcc caaggagcag
     5581 acccgcgcca atatcgcgaa aatcatcgaa accgtgcaga aggaaaacat ccccgccgtc
     5641 ctcgtcggcg tgccgcacat cacactgggc gcgttgttcg gacatttgag cgatcatccg
     5701 ctgtatgagg atttgtccga ggaatacggc attccgctgt tcggcggcgc gtgggcggaa
     5761 attttgggcg ataataatct gaaatccgac caaatccacg ccaacggcaa aggctatcgg
     5821 aaatttgccg aagatttgaa tcaatttttg agaaaacagg ggtttagata aacaaaggtt
     5881 tatccgcacc caagttgttt atataatcat gaaccgactg gaacaccaaa ctgcttcggg
     5941 acgcatatgc cgtctgaagt gcaaaaccta cgccatacag ccgcatgaag ttgcggcggt
     6001 atggcgtttt ttgaaaaaga tggtctgccg gttcagacgg catgaccgac cgtccgaacc
     6061 tgctgtggat aaagcccgga caggctgaaa tcatggaata ttgcgaacct gaagaagcat
     6121 ccgacccgta cgcaacatac aggcgtgcca acctgatggc gggtctgccg ctgtttgtcg
     6181 tgattttggt tctgctcaat attgtttttc cgcttccggc gtatccattg gcttggctgg
     6241 tgcctgcagg tttcatggtt ttgggcggcg gctttccctt atcgctgccg cttgtggcgc
     6301 tgcttgtcct gacctgctgc attctggcgc attgtccgcc attatcccgt cttttgtgct
     6361 gcccttgccc gaatcatccg atgtctaaaa attctgcctg atggcagccc tacaaacccg
     6421 aaggagtaga aatgaaacta tctgaattgt tcaacccgaa cgaatttgcc gcgcgccatt
     6481 tgagtttcgg cgacgaagcg gcgttgcttg ccgctgtcgg cgagaaaagc atggacgaat
     6541 ttgtcggcaa taccgtgccg caaagcatcc gtatgccgtc cgaactcgat ttgcccgatg
     6601 ccctgaccga agcggacgcg ttggcgaaat tgaaaggcat tgcgtcgaaa aacgtgatca
     6661 acaagtccta tatcggtttg ggttattacc cgacccgcgt gccgaatgtg attttgcgca
     6721 acgtattgga aaatccgggc tggtataccg cctacacgcc gtatcaggcg gaaattgccc
     6781 agggtcgttt ggaagctttg ttgaacttcc aacaagtgtg tatcgatttg accggtttcc
     6841 ctgtggcggg cgcgtctttg ctggacgaag cgaccgccgc cgccgaagcg atggcgatgg
     6901 cgcaccgcgt gggcaaggtg aaatccgagc gtttctttgt ggatgcgcgc gtgtatccgc
     6961 aaactttgga cgtgatgaaa acccgcgcca aatatttcgg cttcgagctg gtggtcagcg
     7021 attttgccaa agcggatgac ggcgaatact tcggcgcgct gttccaatac gtcggcaaag
     7081 acggcgatgt gcaagacttg caggatgtta tcggtcgtct gaaagcaaaa ggcacgatcg
     7141 ttgctgttgc cgccgacatc atgagcttgg ttttactgaa atcacctgcc gaattgggag
     7201 cggatattgc tttgggcaac acccaacgtt tcggcgtgcc tatgggcttc ggcgggccgc
     7261 acgccgctta tttcgcgttt aaagacgcgt tcaaacgttc cgcccctggc cgcatcatcg
     7321 gcgtatccaa agacgcatca ggcaaacctg ccttgcgcat ggcgttgtcc acccgcgagc
     7381 aacacatccg ccgcgaaaaa gccacgtcca atatttgtac cgcacaagca ttgctggcga
     7441 atttggcggg tatgtatgcc gtttaccacg gccctgaagg cgtgaaacgc atcgccaacc
     7501 gcattcacgc gctggcttct gcttttgccg atgcgctggt ttcagacggc attaatgtgg
     7561 ttcacaaagt cttcttcgat accgttaccg tcgatttcgg caacaaagag aaagcagacc
     7621 aagtgtttgc cgctgctttg gaatcgggct ataacctgcg ccgcgttaac gatactcaag
     7681 ttgcggctgc attccacgaa acatcggcat gcgaagattt ggttgatttg taccgcgcgt
     7741 ttaccggcaa ggatacgttc gcatttgccg atgatgtcaa aggccgtctg aacgccgaac
     7801 tgctgcgtca agacgacatt ctgcaacatc ccgtgttcaa ccgttaccac accgaacacg
     7861 aaatgctgcg ctacctgaaa aaactcgaag accgcgactt ggcgatgaac cgcagcatga
     7921 tttcattggg cagctgcacc atgaaactca acgcgactgc ggaaatgttg ccgattactt
     7981 gggcggagtt ctccgacatc catccttacg ctcctgaagc gcaaaccgcc ggctaccgcg
     8041 aattgctcgc cgatatggaa aacagcctga aatccatcac cggttttgat gcaatttcct
     8101 tccagcccaa ctccggcgcg cagggcgaat acagcggtat gcttgccatc cgccgctatc
     8161 aggaagcgca aggcgaagcg cagcgcaaca tctgtctgat tcccaaatcc gcccacggca
     8221 ccaaccccgc caccgccgcc atgctcggtt tgaaagtcgt cgtcgtcgat accgacgaac
     8281 acggcaacgt caacatcgac gatttgaaag ccaaagccga gcaacaccgc gacgctttgt
     8341 ccgccatcat gattacctat ccgtccaccc acggcgtgta cgaagaaggc atccgcgaca
     8401 tctgccgcat cgtccatgaa aacggcggac aggtttacat ggacggcgcc aacctcaacg
     8461 cccaaatcgg catcatgcag cctgccgaag tcggcgcgga cgtgttgcac atgaacctgc
     8521 acaaaacctt ctgtatccct cacggcggcg gcggtccggg catgggtccg attggcttga
     8581 aagcccatct ggctccgttc gcaccgggac acgctttgac cgacacccac agcgccagtg
     8641 ccgaccaaac cgccgttgct gctgcggcct tcggttctgc gtccatactg ccgattactt
     8701 ggatgtacct gaccatgatg ggcaaacaag gcatggaaca ggcgacacgc tgggcattgc
     8761 tcaacgccaa ctacgttgcc aaacgcctga gcgaagacta tccgattctg tatacaggca
     8821 aaaacggccg cgtcgcgcat gaatgtatcg tcgatttgcg tccgctcaaa gccgaaagcg
     8881 gcatcactga aaccgacatc gccaaacgcc tgatggacta cggcttccac gccccgacgg
     8941 tttccttccc cgttgccggt acgctgatga tcgagccgac cgagagcgaa agcaaagccg
     9001 aactcgaccg cttcatcgcc gccctgaaac aaatcaaaca ggaagtgctg aaagtcgggc
     9061 gcggcgaatg gccgaaagag gacaatccgc tggtcaacgc tccgcatacc gcatccgatg
     9121 taaccggcga atgggcgcat ccgtactccc gtgaagaagc cgtctttccg ttgccattcg
     9181 tccgcgaaca caaattctgg ccgagcgtca accgtgtgga cgacgtgtac ggcgaccgca
     9241 atctcgtgtg cagctgccca ccgatggaaa attatgaaga ctgaccgttg aaaccccgaa
     9301 gacaaatgcc gtctgaaaca ttttcagacg gcattttcgt caacggcaga ctggttgcac
     9361 caatacacgt atctcgacta taactttaaa acaaatgagt taaaccagta tccatacatc
     9421 agctttttta tcatcctact ttttattcat ccgatcgtgc aaacagattt caaagatgaa
     9481 aagcctattc acaccctttg atgtcatttc cacacggaca aacaaatata gtggattaac
     9541 aaaaaccagt acagcgttgc ctctccttag ctcaaagaga acgattctct aaggtgctca
     9601 agcaccaagt gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct
     9661 gatttttgtt aatccactat aaattcccat aaaaaaacgg agcagatacc tgccccgttt
     9721 ttatttaatc cgaaatttta atctaaattt agaattttgc accggattgg tttgccatat
     9781 agtcaacagc cgctttgact tcgtcatcgc tcaaaccggc attgccgcct ttggccggca
     9841 tcgcgttaaa gccttcaagg gcgtgtttgt gcaaggtttc tttgcctttt ttgatacgcg
     9901 gtgcccaatc gtcttttttg cctatgccgg gaataccggg aatcgaaccg ccgtggcaca
     9961 cctgacaggt tgcttcgaag acttttttac cgtcaacgcc gaccgcaggg gctgccgcac
    10021 ccttgtcttc tgccttcgct tctgccggag ctgcactatc ggcaggagca gaagctgttc
    10081 ctgaagcggc attgtcggca ggcgcagcct catcaggatt cgggaaagaa ccgccgcttt
    10141 tgttcgccat gtaagtaatc gcccgtttga gttcctgatc ggtcaggtct gccgcaccgc
    10201 ctttggcagg catggcgtta aagccgttca gcgcgtgttg gaacaaggta tcgaagcctt
    10261 gcgcgatacg cggtgcccaa tcgccgttgt gttccagttt cggagcgttc ggcacattgc
    10321 tgtccgccgc gtggcattgg atacagattt tgccgaaaat ctgttcgcct tggcgttcgc
    10381 cgacggggat gccgtcgccc atcgtcaatt gtccgacagg ctggatacgg gtctgcgttg
    10441 ctgcttccgt agtggcatcg acatcgccga acgagccgct gcccgccagc ttaatcagga
    10501 aataaaggac tgcaataaca ataacgatac cgctcacaag ggtaaacagt gcagagcctt
    10561 gggctttgtt gtcgcggagt tgtttcattt ggtaggcctc gccgtcaggt taggttgtgc
    10621 tgtaaattat agtttggtgt gttaaacgca gttaacaata ttttgctgga ttatactgaa
    10681 ttcacagggt ctttccaatc gctatcattg aaaatatgaa aaaatttgcc aacggtatct
    10741 gtataaaaca aataatcctt tgaaaataat ggtttatcct caagaaaact ctccttatgc
    10801 cgccatacgc cgcctgccgg cgcaagataa cctttgccaa tttgcagaat ttacgttaac
    10861 cttacgtttt acgcacccat agctcagttg gaagagtgtc agtttccgaa gctggaggtc
    10921 acaggttcga tccctgttgg gtgcgccaat tataaagaga ccgtctgaaa gataaatatt
    10981 tttcagacag tcttttgact tacttcaaac tcttatttca agacttccgc aaatgcgcgg
    11041 gcaacatagt cggtattcga cgtattcagt ccggcgacgc acatcctgcc ggaatccagc
    11101 aggtaaacgg caaattcgtc gcgcagcctg cggacttgtt ccacgctcaa tcctgtgtag
    11161 ccgaacatgc cgcgctgttt gatgaaataa gtgaaatcgc gatcggggat ttgcgcagtt
    11221 aagacatcat aaagtttctg ccgcatcgca cggatgcggt cgcgcatcat ataaacctcg
    11281 ttttgccaca aggcgtaaag ttcggggctg ttcatcacgt cggcggcgat atacgcgccg
    11341 tgcgcgggcg ggctggagta gatgcggcgg acggtgaatt tgagctgtcc gaacaccaaa
    11401 tccgcttctt ccttattcgg gcaaaccacg cttaagccgc cgacgcgctc gccgtagagc
    11461 gacaggtttt ttgagaagga attgctgacg aacaagggca attccatttc caccgctttg
    11521 cggacggcgt aggcatcgct gtccaaatcg ccgccgaagc cttggtaggc gatgtccata
    11581 aacggaatca gtttgcgcgt tttgatgatg tgcaacactt cgtcccattg ttgttccgac
    11641 atatccacgc cggtcgggtt gtggcagcag ggatggagga tcaggacgct gttttcgggc
    11701 agggtgttga aaaacgcggt catttcgtcg aatttcacgc cgacagtggc agggtcgtaa
    11761 tatgggtaag tgccgacctc gaaacctgcg ccttcaaaaa tgccgcgatg gttgtcccaa
    11821 gtcgggtcgc tgacgtaggc gcgcgcttca ggaaaccagc ggtgcaggaa atctgctccg
    11881 actttgagcg cgcccgagcc gcccaaggtt tgtacggtag cgatgcgtcc ttgcgcgagc
    11941 gcagggttgt ctttgccgaa caataaatgc tgcaccgcgc tgcggtaagt gtccaagcct
    12001 tccatcggca ggtagggcga cggcgcaggc gcggcggcac gggcggtttc ggctcggctc
    12061 acggattcca acacgggcat tttgccttcg tcgtcgaaat aaatgcctat gctcaaattg
    12121 actttttcgg ggcgcgggtc gtttttgaag gtttcgacca aactcaaaat cgggtcgccg
    12181 ggatagtatt cgatgtgtcg gtacatagtc cttacctctt gctttttcaa aggattttct
    12241 ttttcaacaa tacaccactt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg
    12301 cgcgttcgac cacatgggtt tccaccaagg tgtccaaatt ggcacgcagc gtttcgggat
    12361 tgcaggaaat gtagatgatg ttgtcaaact gcgacaccag cttcaaagtt tcctcatcga
    12421 taccggcacg cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaagcaatac
    12481 cgccatcctt aaggcgtgca aactcacgtt tgccggtata ggcttcggta aattcctcgg
    12541 cagacagacg ggcgattttg atgttgccta tcccgtttgc ctcaatattc cattgcgccg
    12601 cacctactga agttttggaa atctcggttg ccaaaacacg ttcaaacttt tcagataaag
    12661 gcagggtgaa attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg
    12721 ccgcaccgca tgcccattca agcatttttt gacacacggc ggcattgggt tgggtaaaac
    12781 cgccttcaat ttgccgataa cgaaaatccc gattaccgac ctttaaagtt tccgttacat
    12841 agtcctgttt caagactatt ttctgccccc tgctccgccc aataacggaa gtatccaact
    12901 gttgctgtaa cgcttgcgcc gcctgcatcc actcatcatc aagccttttg tggtaaatca
    12961 tggtaaccag catttccccg ctgagcgtag acaaaaattc gacggcatac caacggtttc
    13021 tcagttcggc agattgggcg gcaacggcaa tcaattctgg catcaggcgg ttgacggctt
    13081 cggaagccgc ttcaaaacgg tcgcaacgga tcaggcttgc cccgccagcc ttttgtcctt
    13141 tttcaaacat ggcataaaac atttcaccgc cttcgtgcca aatacggaac tcggcacgca
    13201 tacggtaatg tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa
    13261 aaagggtttt aaggtggtct tttttatctt gaagctgctg cgtgtagtcg ctcatatcgc
    13321 tatcctgaaa aatcagaccg gattataagc cgatttgcct cgccgcacgc aaactctttg
    13381 actgccgccc cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact
    13441 ataccgataa agggaaaaat catggcaggc aacactttcg gacaactctt caccgttacc
    13501 accttcggcg aaagccacgg cgcgggtttg ggctgtatca tcgacggctg cccgcccggc
    13561 ttggaattaa gcgaagcgga tatccaattt gacctcgacc gacgcaaacc cggcaccagc
    13621 cgccacgtta cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa
    13681 ggcaaaacca ccggcacgcc catcgccctc ttaatccgca ataccgacca gcgcagcaaa
    13741 gactacggca acatcgccac cagcttccgc cccggccacg ccgactatac ctattggcac
    13801 aaatacggca cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgccgcc
    13861 cgcgttgccg ccggagccgt tgccaaaaaa tggttgaaag aaaaattcgg cacggaaatc
    13921 accgcctacg ttacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgaacacatt
    13981 tcccaaaatc ctttttttgc cgccaaccat agccaaattg ccgagctgga aaactatatg
    14041 gacagcgtgc gcaaatcctt ggattccgtc ggcgcgaagc tgcatattga agccgccaac
    14101 gtccccgtcg gcttgggcga acccgtattc gaccgcctcg atgccgaaat cgcctacgcc
    14161 atgatgggca tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg
    14221 caacgcggca gcgaacacgg cgacgaactc accccgcaag gcttcctgtc caaccactca
    14281 ggcggcatcc tcggcggcat cagcaccgga caagacatcc acgtcaatat cgccatcaaa
    14341 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
    14401 ctcgccacgc acggcaggca cgacccctgc gtcggcttgc gcgccgcgcc gattgcagaa
    14461 gctatgctgg cattggtttt aatcgaccac gccctgcgcc atcgcgcgca aaatgcggat
    14521 gttcaggtta atacgcccga cattaccctt tcaaacaaat aaaaatttag ccaaaacaca
    14581 gactttataa tagaataccg agcattgccg tgccgctttg acgcacgggt ttgtttagag
    14641 gaatacaacc gaaatgacac aagaaaccgc tttgggcgcg gcactgaaat ccgccgtcca
    14701 aactatgagc aaaaagaaac agaccgaaat gatcgccgac cacatctacg gcaaatacga
    14761 tgtattcaaa cgcttcaaac cgttggcgct cggcattgat caggatttga ttgccgcttt
    14821 gccgcaatac gattccgcac tgattgcacg cgtcctcgcc aaccactgcc gccgtccgcg
    14881 ttatctgaaa gccttggcgc gcggaggcaa acgtttcgat ttgaacaacc gtttcaaagg
    14941 cgaagttacc cccgaagaac aggcgatcgc gcaaaaccat cctttcgtgc agcaggcttt
    15001 gcaacagcag tccgcccaag ctgccgccga agcagccgga tcttccgcag cagaataaat
    15061 ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt ttcgtatgcg
    15121 gcaatcacgg ttcaaatatc caattccgcc gtatcgcctt cgcgttccat ccaagcgcgg
    15181 cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgtgt ttcgtcatct
    15241 gcgccttctg ggatttgtac ctgcaacaga cggcgggtgt cggggtgcat ggtggtgtct
    15301 ttgagttggt cggggttcat ctcgcccaag cctttaaaac ggctgatgga ataagcagtt
    15361 tctttaaagc cttctttttg tagtcgctcc aaaatgctgt cgagttcgtt ttggtcgagg
    15421 gcgtagaatt tgcgggccgg tttgtttttg ccttgtgcat tgacatcgac gcggaacagc
    15481 ggcggctggg cgacgtagat gtgtccatcg gcgaccagtt tcgggaagtg gcggtagaac
    15541 aaggtcagca gcaaaacttg aatatgcgag ccgtccacgt cggcatcgga caggatggcg
    15601 attttgccgt agcgcaggcc gcttaaatcg ggatggtcgt taataccgtg cggatcgacg
    15661 ccgatggcga cggaaatgtc gtggatttcg gcgttgccga agagttggtc ggggtggact
    15721 tcaaagctgt tgagcacttt gccgcgcagg ggcaggatgg cttgggtggc tttgtcgcgg
    15781 gcgagtttgg ctgagccgcc ggcggaatcg ccttcgacga ggaagagttc gttttcgcgg
    15841 atgtcttcgc tttcgcagtc ggtcagtttg ccgggcagga cggcgacgcc gctgcctttt
    15901 ttcttttcga ttttcttaac cgaacgcatc cgcgcctgcg cctgacggat ggcgagttcg
    15961 gcgatttttt taccgaagtc cacgttttgg ttcagccaca attccaaagg gtcgcccgat
    16021 acggcggcaa cgagtttcaa cgcgtcgcgg ttggtcagct tgtctttggt ttgaccttgg
    16081 aactgcgggt cgaggacgcg ggcggagagg acgaaggcgg ttttaccgaa cacgtcgtca
    16141 ctttgtactt tcacgccgcg cggtaggagg ttgtgcagat tgatgaaatt gttgacggcg
    16201 ttgaacacgg cttgtttcaa acctgcttcg tgcgtgccgc ccagcggggt ggggatgagg
    16261 ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aggtcagggc aaacgccgct
    16321 ccttcgccga tgctgaaatc gccgttgtgg tcgtctgaaa tgtagttttc gcaagagaag
    16381 ataggcacgg cttcctgcgc gtcggcaatc aggtcggtca gatagctttt cagaccgtcg
    16441 gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg ggtcagggaa
    16501 acgcgcacgc ccggcagcag cacggctttg gcgcgcagca ggcgttcgag ttcggggatg
    16561 ctgtaattcg ggctttcaaa atatttgccg tccggccaga cgcgcacttc gatgccgctg
    16621 tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacgtcgcc gccggcaaac
    16681 acgatgcggt ggattttgcc ttcgcgtttg accgttactt caaggcgggt ggaaagggcg
    16741 ttggtgacgg atacgcccac gccgtgcagg ccgcctgaaa aggcatacgc gctgccgccg
    16801 tcttttttgt tgaacttgcc gcccgcgtgc agacgggtga atacgagttc gactacggat
    16861 acgccttctt cgggatgcag gccgacggga atgccgcgcc cattgtcgtg cacggaaagc
    16921 gaaccgtctt catgaatttg cacgtcgatt gcagtcgcga aaccgcccaa cgcttcatcc
    16981 gccgcgttgt cgatgacttc ttggcagata tgggtcgggc tgtcagtgcg ggtgtacatg
    17041 ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga ttcgctgtat
    17101 tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt tcagacggcc
    17161 tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc ggcaaagtct
    17221 tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc ccccaaataa
    17281 acgtcgcgtt tgaactcgat gacttcgtca acgggcgccc aatattgatg ccaacgccag
    17341 ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg gccggtcagg
    17401 cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc gcggcgcacc
    17461 cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat tttgacgtgt
    17521 tgcggcagaa gtccgacttc ttcgtaaagc tcgcggtaca tcgcggtttc ggggctttcg
    17581 cccggcttga tgccgccttg cggaaactgc caagaatgtt cgcgcacgcg cttaccccaa
    17641 aagacttcgt cgcggttgtt ggttaagatg ataccgacat tggggcgata gccttctctg
    17701 tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca aatcgaacgg
    17761 ttttgacaaa tcagacggta tggttcaagc atgtcgaaac acagacggac tcgggaaaat
    17821 atcttaaatt tagtttacaa taatacgttt caaattattc gggaatcaga ccatgttaga
    17881 tatccaattg ctccgcagca acaccgccgc cgttgccgaa cggcttgcac ggcgcggtta
    17941 tgactttgat accgcacgtt ttgacgcact ggaagaacga cgcaagtccg ttcaggtgaa
    18001 aactgaagaa ctacaagcct cgcgcaacag catttccaaa caaatcggcg cactgaaagg
    18061 tcagggcaaa cacgaagaag cgcaggcggc gatggatcaa gtcgcccaaa tcaaaaccga
    18121 tttggaacag gctgccgccg atttggatgc cgttcaaaaa gaattggacg catggttgtt
    18181 gagcatacct aacctgccgc acgaaagcgt acctgccggt aaagacgaaa ccgaaaacgt
    18241 cgaagtccgc aaagtcggca ccccgcgcga atttgacttt gaaatcaaag accatgtcga
    18301 tttgggcgaa cctttgggtt tggatttcga aggcggcgca aaactctccg gcgcacgatt
    18361 taccgttatg cgcggacaaa tcgcccgtct gcaccgcgcg ctggcacaat tcatgctcga
    18421 tacgcacaca ctgaaacacg gctacaccga gcattacacg ccctatattg tggacgacac
    18481 caccctgcaa ggtacgggcc agctgccgaa gtttgcggaa gatttgttcc acgttacccg
    18541 tggcggcgac gagagcaaaa ccacgcaata tctgattccg acagctgaag ttaccctgac
    18601 caataccgtt gcaggcagca ttttagacga aggcggactg ccgctgaaac tgaccgccca
    18661 ctcgccctgc ttccgcagcg aggcgggttc gtacggcaaa gacacgcgcg gtctgattcg
    18721 ccagcaccag ttcgacaaag tggaaatggt tcaaatcgtt catcccgaaa aatcatacga
    18781 aacgctggaa gaaatggtcg gccatgccga aaacatcctg aaggctttgg aactgcccta
    18841 ccgcgtgatt accctgtgta ccggcgacat gggcttcggc gcaaccaaaa cgtatgactt
    18901 ggaagtttgg gtaccggcgc aaaacaccta ccgcgaaatc tcaagctgct ccaactgcga
    18961 agatttccaa gcccgccgca tgaaggcgcg tttcaaagac gaaaacggca aaaaccgctt
    19021 ggtacatact ttgaacggct ccggcttggc tgtcggcaga acgctggtcg ccgtattgga
    19081 aaaccatcaa aacgccgacg gcagcatcaa catccctgcc gcactgcaac cgtatatggg
    19141 cggcgttgcc aagttggaag tgaaataagt ttgcaagctg cctgaacgtc aaatgccgtc
    19201 tgaaacctgg ttcagacggc atttccttta aacttttaaa acacatcaac cgtcggcacg
    19261 aaccgcattg ccgcaatcgc cggtctgtcc gacctcgcgg atattggaca gcgtaacttc
    19321 cgaaatatta cccaacgcct cttccgtcaa aaatgcctga tggccggtaa acagcacatt
    19381 atgacaagac gacaggcggc ggaacacgtc gtcggtaatc acatcgttgg atttgtcttc
    19441 aaaaaacagc tcgcgctcgt tctcgtacac atccatgccc aatgcgccga ttttccggcg
    19501 tttcaacgcc tcaatcgcgg cggcactgtc aatcagcccg ccacggctgg tgttgataat
    19561 catcacgccg tctttcattt tgtcgaacgc cgcttcgttc agcatatagt ggttttccgg
    19621 cgtggcgggg caatgcagcg tgatgatgtc cgaccgggca tacagctcgt tcaactccac
    19681 atacctgccg ccgatttttt ccgcttcggg gttgcaaaac ggatcgtaag ccagcaggtt
    19741 catgccgaaa ccctttaaaa tccgcatcgt tgcgataccg attttccccg tgccgataac
    19801 gcccgccgtt ttgccgtaca tattgaaacc ggtcagacct tccagcgaaa aattcgcatc
    19861 gcgggtacgc tgataggctt tgtggatacg gcggttcaac gtcagcatca gaccgaccgt
    19921 atgttccgca accgattcgg gcgaataggc aggcacgcgc acgactttca agcccaactc
    19981 ttcagccgct ttcaaatcca cattattgaa gccggcacaa cgcaacgcca cagttttcac
    20041 gccaatttgc gccaattttt ccaacacggg ccggctgccg tcgtcgttta caaaaataca
    20101 aaccgcttcc gcgccttccg ccattttcgc cgttttcgca tccagcataa aatcaaaaaa
    20161 ctccagctcg aagccaaaat gccggttggc gcgggtaaaa tgttcgcggt catagctttt
    20221 cgtaccgtaa atcgcaatct tcatcaatat gtccagttgt cgtctatggt tgagaaacgg
    20281 cataatacac tgaattcaaa caaatcagta gaatatggtg gattaaaatt gattgcatgc
    20341 acggcatttc catttcaaaa cacaaaactc aatcgcccat tgccgccaaa agctccgcct
    20401 gatgttcggc aatcagggca tcggtgattt ctgccaaatc gccgtccatc acaaaatcca
    20461 gcttgtgcag ggtgaggttg atgcggtggt ctgttaccct gccttgggga tagttgtagg
    20521 tgcggatgcg ttcgctgcgg tcgccgctgc cgatgaggga tttgcgttcg gcggcttctt
    20581 tggcttgggc ttcgcgtttt tgcgcgtcgt tcaggcgggc ggcgagaact ttcattgcct
    20641 gcgctttgtt ggcgtgttgg ctgcggccgt cttggcattc gaccaccatg ccggtgggca
    20701 ggtgggtaat gcggacggcg gaatcggttt tgttgatgtg ctgaccgcct gcgccggatg
    20761 cgcggaaggt gtcgatgcga aggtcgacgg ggttcaactc gatgtcttcc agttcgtccg
    20821 cttcgggcat gacggcaacg gtgcaggcgg aggtgtggat gcggccttgg ctttcggtgg
    20881 cggggacgcg ctgcacgcgg tgtccgcccg attcaaattt caaacggctg tacgcgccta
    20941 agccgataat acgggcgata acttctttat agccgcccaa ctcgctttcg ttggcggaca
    21001 cgatttcaac ctgccagcgg ttgcgttcgg cgtagcggct gtacatacgc agcaaatcgc
    21061 cagcaaacag cgcggcttcg tcgccgcccg ttccggcgcg tacttcgata aagatgtttt
    21121 tgtcgtcgtc ggcatctttg ggcagcagca gtttttgcaa ttcggtatcg agtgcgccga
    21181 ttttggcttt cgccgcttcg atttcttcgg cggcaaagtc tttcatttcg gggtcggaca
    21241 acatttcttc ggcatccgcc aaatcgcttt gcgccagttg atagttttgg aatacttcga
    21301 cgacgggggt cagttcagcg tgttcgcgcg tgagcttgcg gtagttgtcc atatcggacg
    21361 tggcttcggg ttgccctaaa aggtgggtaa cttcttccaa ccggtcgccg agttgttgca
    21421 gtttttctaa aatagacggc ttcataattc ttccataaca aacgccgcct gaagttcaga
    21481 cggcatcaaa acgcggtcat tataatatat tttctgaata ttcaaaaaga taatctaggg
    21541 acggagcagg ctgtcggcat tgcgttccgt tatcgcggcg atgtccgcca cgccgacacc
    21601 gcgtatctgc gcggcaattt cggcaatcca aagaatattg gcaggcgtat taatctcttt
    21661 tttcagcata aacgggctgt cggtttccaa tacgaaatcc ccgtcgttca aggctttaag
    21721 cgtatcgcgc actttacgcg cgttcggatt gagcaacaac gaaccgatgc cgattttgaa
    21781 acccagtttc gtcaacacgc gcgcttcttc cgcgctgcct gagaaggcgt gaacgatgcc
    21841 gccttgggca aagcctgtct gtttgacggc ggcggcgatg tcggcggtgg ctttgagatt
    21901 atggataatc acgcggcggc gcagggtttg cgcgatggca agctggcgga cgaaaacttg
    21961 gatttgccgt tcgcgctgcg gcggcgtttg ggttttgtcg taaaaatcca agccgatttc
    22021 gccgacccat gcctgcggat aacgtgccaa catcgcttcc aggcggacgc aatcacgctc
    22081 ggcaatgccg tctgaaaacc aaggatggat gccgagcgca atacggattt gaccgtgttc
    22141 ggacggcatt tccgacagtt ccgccacgct ttgccaatcc tgcggacagg tggcgggaac
    22201 gataaaccgc ttcaccccaa ctttccgcgc ggcggtcagg atgtgcggca ggttttcgcg
    22261 cagggcggca tcggcaaggt ggcagtgggt gtcggtgaag ttcatttcga ttccgcaatg
    22321 cggcaggttc acataaaaat aaggcagggc agcaagggat gccgtgtacg ggcggcgcat
    22381 aaggcgcggt caacgccccg tcattgccat ttcaatccgc tataacttca gtcttaccaa
    22441 ctctttttcc acctcttcct taccccagcc ttgcgatacg gcgagggtca taagcgcggt
    22501 ggcggtttca aggttgcatt tgccgccgtt gatgatgccc gagttgcgga acgcattgcc
    22561 ttgcgcgtaa acggcggcgg ttttgccttg tcggacttgg ctgatgttga gcaacaattt
    22621 gccttgctgc gtgaaatctt gcacggcgcg gataaaacct tcgtctgcgg gggtgttgcc
    22681 gtgtccgtag ctttgcagga taagggcttg ggcgggaagc tgtccgagtc cgtccgcaag
    22741 ttcttggacg gcaaagccgg ggataagcgt gcggacggcg attttcgcct gcgggtcggg
    22801 ataacggatt ttgaggccgt ctgaaacatc tgccgcgtcc tgattcggga tgcggacatt
    22861 gtgccagccc tgggtttcgt cccactccgc cagtgcgccg aaatgcggat tgtcgaaacc
    22921 ggcggcggtt tcggtgctga ctttgctgct gccgacggcg ggataaagtt tgccgtcaaa
    22981 ggcaataagc gtctgcttca atccgagtgg gaatgcggca acggcagtcg ccagattgcg
    23041 cggcgcgtcg ctgcccacgg cgttgtacgg ccattgggag ccggtcagga cgattggctt
    23101 gtccaaaccc tgcagggcga gggcgaacag attggcggta taggcgagcg tgtccgtgcc
    23161 gtgcagcacc aaaacgccgt cgtactgcgg cagcgcggcg gcaatgatgt ccagccaagc
    23221 gcaccagttt tgcagttgga cggcggaaga atcaatcagc ggcccacaaa cgtgccaatc
    23281 gaaatccatg ccgtttgaaa acggcgcgag tgcctgcccg accagcgcgg tatcggggcg
    23341 caggccttcg ctgctttgcg tcatgccgat ggtgccgccg gtataaagga caaaaatttt
    23401 ctgtttcatg gctcatcctg aaaatgcaaa gaccgcctga aacgcgaaat gcgtccggac
    23461 ggtcgaaaat ctgatttact gctgcttctg tgccgctttt ttcgctgcct tttccgcctt
    23521 gcgtttggcg cgtttttcgc tcaattgtgc gcggtaaagc tgataatgtc ggcgtttgcg
    23581 ccaccagaac cacgccagcg ccgccgccag cacgcccaat gcgatgaaga tgccggattg
    23641 caggctgtgc attttcgcca tcagccaatc gatgttgtgc gcgccgtact cgcccaagta
    23701 aatccaaacg ggcacggaaa tcagcgcggc aagcccgtcc ataatcagaa agcgcagata
    23761 cgatactttg cggctgatgc cggcggtaac gaaaacggca gtccgcaaac ccggcaggaa
    23821 gcgggcgaca aacagaaccc agttgccgta tttgtcgaat ttttcctgaa cctgtgcgta
    23881 acgtttcggc gtcatgatgc gcgcaatagg tttgaacttg aggattttct gcccccagat
    23941 gcgtccggcg gcgaacatga tgccgtcccc gaccaatacg ccgagcatac cgactgcaaa
    24001 cataatatgc ggattggtat aacccatacc cgaaatcacg ccgcctgtta ccaaggtcaa
    24061 atcctcggga atcggcacgc cgaaaccgca gatgaccaaa acgaaaaaca cggccgcata
    24121 gccgtattcg acaaaaaagg cttccaaaag ggcaaacata gcggatattc cattgtcgga
    24181 gataaaaagt cagaacaagc cgaaacattt tctgcgcgaa acggcattct atcaaagatt
    24241 atgccgtctg aaagcggcaa ggggcagatt cgcgccctct gccggacgtt cagacggcat
    24301 ttaaacagga aaaccgccgc tgttgaaacg gttttgcctg attttgccta aaggctgccg
    24361 atggcggaag caatacgttc cgcaccgtcg cgcgcccaat ccgcctgccg cgcctcaacc
    24421 atcactcgca cgacgggttc ggttcccgaa gcgcgcaaca cgacacgccc tttgccttcg
    24481 agttcttttt ccacttccgc caacacgtct tttgaggctt cctgccattt ctgacctttt
    24541 tggatgcgca cgttaatcat agtttgcgga tacggctgcc aatcggcgca aacggtggcg
    24601 aggtcttggt tcagcgtttg cagtgccgcc aaaacttgca gcgcggaaat aatgccgtcg
    24661 ccggtgttgt gtttgtccat gcacaaaata tggccgctgg cttcgccgcc gatgagccag
    24721 ccgcgttggt tcagctgttc caacacatag cggtcgccga ctttggcgcg gcagaaatcg
    24781 acgccctgct ctttcagggc gatttccatc gccatattgg tcatgaccgt gccgaccacg
    24841 ccgccgatgt tgataccttc tcgggcgcgg gctttagcaa tgacgtaaat caggctgtcg
    24901 ccgtcgtaaa ccttaccgtt tttatcgacc atcatcaggc ggtctccgtc gccgtctaag
    24961 gcgatgccgt agtcggcttc atgctgtaaa acggctgcct ggagtgtctt ggtataagtc
    25021 gcaccgcatt tttcattgat attgtagccg ttgggttcgt cgccgatgct gacgacttgt
    25081 gcgcccagtt cgtgaaacac cttgggtgcg acaccgtacc ccgcgccgtt ggcagtatcg
    25141 ataaccaact tcaaaccccg aaggtcggaa tggctgggaa aggtagattt gcaaaattcg
    25201 atatagcggt cgtccgcacc actgatgcgg cgtgcgcgac cgaggcgtgc agaaggctgg
    25261 gttttcattt cttcgtcgat tttggcttcg atttccaact cgatttcatc gctcagtttc
    25321 acgccgccct cggcaaagaa tttgatgccg ttgtcggaat aggcgttgtg cgacgcggaa
    25381 atcatcacgc cggcggacag gcgcaacgcg cgggtcagat aagccacgcc gggcgtgggc
    25441 agcggtccgg tctgtaccac attcacaccc gccgccgtaa aaccggccac caaagcggct
    25501 tccagcatat aaccggaaat acgcgtatcc ttgccgatta aaacggtcgg tttctggtca
    25561 gtgtcgtgct gaaccaaaac ctgacccgcc gcatagccga gtttcaatac aaaatcgggc
    25621 gtaatcggaa actgcccgac ttcgccgcgc acgccgtccg tgccgaaata tttttttgcc
    25681 atgtgttgct ccgagaatgt gaaccgttgt ccgagattat acagtcagtt tgtgccttgc
    25741 tgtctgcacc gttgatgccg tctgaaaccg ccccgtcctt ttcagacggc atgaagtatg
    25801 tgaaccgctg tttacagatt gatgcccaac gcttcccaca ccttcaacgc atccgctgtc
    25861 gccttcacat catgcacccg cacgatttgc gcgccgcgcg ctacggaagc caacgctgcc
    25921 gccacgctgc cgtgtacgcg ttccgccgca tttgcctcgc cggtcagctc gcctatcgtg
    25981 cttttgcgcg atacgccgat gagcagcgga aaacctgttt ccgccatcaa ttcgggcaaa
    26041 tgccgcatca gcgcgatatt gtgttgcaag ggtttgccga agccggagcc gaagccgggg
    26101 tcgagtatga tgcgttgcgg tgcgatgcct gccgcgatac attccgctga gcgcgctttc
    26161 aaataccgcg ctacttcacc gacaacatct tgatatttcg gattaatctg catggttttg
    26221 ggcaaaccct gcatgtgcat caggcaaatg cccgtgtccg cctgacgcgc cagcaattcg
    26281 accgcgccct cgtcattcaa cgccgccaca tcattaataa tatcgacgcc gccgagtgcc
    26341 aacgcttttt ccataatcac cgtgcggcgc gtgtccaaac tgatgggaac gccccacccc
    26401 gccacttccg ccaaaacagg ctcaacccgc gcccattctt cttcaggcga aacataatcc
    26461 gcacccgacc gcgtcgattc gccgccgatg tcgagaatgt ccgcaccctc tttcaaaagc
    26521 cgttcggcgt gcgccaaagc tgtttgggca ttttgcgaat acgcgccgcc gtcggaaaaa
    26581 gaatcgggcg tgagattcac gatacccatg attttcggtt tgtccaaacc gatttcaaac
    26641 cgtcctgcct gccaaacgtg tcgtgccatc tgaactcctc ccaaaataaa aaacagatta
    26701 tatgccgtct gaaaccgtct tgtgcgcttc agacggcacc gctattcagg cggcagacgg
    26761 catgttgtcc gaatgtctgc tccgcctttg aatctgccgg tatgcctgct atccgcccga
    26821 cttttcaaaa caggttccga cgattccgca cgcgcctgcc gcctttgcca agccgtacag
    26881 gatttcctgc ggcatatcgc ggttccataa tcccgtaata ttcgcaatca cgggcagatg
    26941 gctgatttgg cggactttca cgatggattc gacatccaaa cggtagggat ggtctttggt
    27001 atggctcaac acgcccgact cgcccagaat caggtggcgg ttgccgcgcg agacaacata
    27061 ttctgcggca ttcaaccaat cttcggcaga atggtgcttg tctttacaca gcaccaaggg
    27121 aatgttcagg cttccggctt cattcaatac ggcaagatcg gacatcagcc cgccgcccaa
    27181 atacaggatg tccgcccccg cattcaaagc cgcttcgaca tggcggacgt tgcggacgcg
    27241 caccaatacg ggtttccctg cgccatgcgc cgatgcggtc tgttccgcca accgtctgca
    27301 ccgtcctcgc ccttcatccg cacttgaagt gtcgtataag tttgccgaag tgaaaaacgg
    27361 atctagaaac actgcatccg cattgcgcca tactgacggt tctgcggcga tacagacggt
    27421 ttccccgccg ccgaaagcca cgcctttggc ggcaacgcgg ctgtcttccg cccgattttc
    27481 ccgactgacg gttttccatg tatccaaaat gcggacggct ttctcgacct ccggcagcgt
    27541 ctgcacctcc ctgacgctca aaaccctatc gtcgccgatt gcgccgatga cggtacgctc
    27601 gtcgccgtga gaaatgtgtt ctcgcagacc tctgctgcgg ataaaggcga caacgccggc
    27661 aatgtccgct tcggcggcac gcctgctcat gacaataatc atatttcctc ctgacacaag
    27721 aaacggcctg cccaaaatag gatttttgca agccgtgtta tactgtggcg tgttttacag
    27781 attgttcggg ctatggattt attatcggtt ttccacaaat accgtctgaa atatgcggtg
    27841 gccgtgctga cgatactgct tttggcggca gtcgggctgc acgcttccgt atatcgcacc
    27901 ttcacgcctg aaaacatccg cagccgcctc caacaaagca ttgcacacac acaccggaaa
    27961 atctcgtttg atgcggacat tcagcgcagg ctcctgcccc ggccgaccgt catcctgaaa
    28021 aacctgacca ttaccgaacc cggcggcgac cagactgccg tttccgtcca agaaaccaaa
    28081 atcggattga gctggaaaaa cctgtggtcg gatcagatac agattgaaaa atgggtggtt
    28141 tcgagtgcgg aacttgccct gacgcgcgac gggaaaggtg tttggaacat ccaagacctg
    28201 atcgacagcc aaaaacgcca agcctcagtc aaccgcatta tcgtcgaaaa cagcaccgtc
    28261 cgcctcaatt tcctgcagga acagcttatc ctgaaggaaa tcaacctcaa cctgcaatcc
    28321 cccgattcgt cggggcagcc gtttgaaagt tcgggcatac tggtttgggg aaagctgtcc
    28381 gtcccgtgga aaagcagggg gctgttcctt tcagacggca tcggcacgcc caaaatctca
    28441 ccgttccatt ttgaagcttc cacttcgctg gacggacacg gcattaccat ttccaccacc
    28501 ggcagccctt ctgtccgctt caacgccggc ggagcggatg ccgccggcct cggcctgcgt
    28561 gcagacactt ccttccgcaa cctccacctg accgcccaaa tccctacgct ggcactcaga
    28621 aataacagca ttaaaattga aaccgtcaac ggcgcattta ccgccggcgg cgaatatgcc
    28681 caatgggacg gttcgttcaa actcgacaaa gccaaccttc actccggcat cgccaacatc
    28741 ggcaacgccg aaatctccgg cagcttcaaa acaccgcgcc accagaccaa cttctccctc
    28801 aattcgccgc tcgtatggac ggaaaacaaa gggctggacg cgccgcgcct gtatgtatcg
    28861 acccttcagg ataccgtcaa ccgcctgccg caaccccgtt tcatcagccg gctcgacggt
    28921 tcgctgtccg taccgaatct gcaaaattgg aatgccgaat taaacggcac attcgaccgc
    28981 caaaccgttg ccgcgaaatt cagatacaca catgaagacg caccgcatct ggaagccgcc
    29041 gtcgcactgc aaaaattgaa cctgaccccc tatcttgacg acgtgcggca acaaaacggc
    29101 aaaatatttc ccgacaccct cgccaagctg tccggcgaca tcgaggcgca cctgaaaatc
    29161 ggaaaagtcc aacttcccgg cctgcaactg gacgatatgg aaacctacct ccacgccgac
    29221 aaaggccata tcgcgctcag ccgtttcaag tcagggcttt acggcggcca taccgaaggc
    29281 ggcatcagca tcgccaacac ccgtcccgcc acttaccgcc tgcaacagaa tgcaagcaac
    29341 atccaaatcc aaccgctgct gcaagacctg ttcggcttcc acagcttcag cggcaacggc
    29401 gatgcggtca tcgacctgac cgcgggcggc gaaacccgaa aagagcttat ccgctcgctt
    29461 cagggcagcc tgtcgctgaa tatttccaac ggcgcgtggc acggtatcga catggacaat
    29521 atcctgaaaa acggcatttc gggcaaaact gccgacaatg ccgcacccag cacacccttc
    29581 caccgattca cgctcaacag cgaaatttca gacggcatca gccgccacat cgataccgaa
    29641 ctcttctccg acagcctcta tgttaccagc aacggctata ccaatctgga tacgcaggaa
    29701 ttgtctgaag atgtccttat ccgcaacgcc gtccatccga aaaacaaacc gattcccctg
    29761 aaaatcaccg gtacggtgga caaaccgtcc attaccgtcg attacggcag gctgaccggc
    29821 ggcatcaatt cgcgcaaaga gaaacagaaa atcctcgaag acaccctgct ggaacaatgg
    29881 cagtggctca aacctaaaga accgtaaaca tcctgcgtac aaaaatgccg tctgaaacac
    29941 ccccgcgctt cagacggcat gaccgtaaaa cctacaaccc caattcctcc caaatcccat
    30001 caatcttagc cgtaaccgca gggtcttttt tgatgacgcg cccccattcg cggtcggttt
    30061 ctcccggcca tttgttggtc gcatccaaac ccattttgcc gccgagtccg ctaacggggc
    30121 tggcgaagtc gagataatcg atgggcgtgt tttctaccaa aacagtgtcg cgcacggggt
    30181 ccatgcgcgt ggttaccgcc cagatgactt ctttccagtc gcgcacgttc acatcgtcat
    30241 ccaccacgat gatgaatttg gtatacataa actggcgcag gaacgaccag cagcccatca
    30301 tcacgcgctt ggcgtgtccg gcgtactgtt ttttcatgcc gatcaccgcc atgcggtagg
    30361 agcagccttc gggcggcagg taaaaatcgg tgatttcggg gaactgcttt tgcaaaagcg
    30421 gtacgaacac ttcgttcaac gccacgccca aaacagcggg ttcatcgggc ggtttgcctg
    30481 tgtaggtcga atggtaaatc gggttttcgc gcatggtgat gcgctcgacc gtaaatacag
    30541 ggaaatgatc ctgctcgttg taatagccgg tgtggtcgcc gtatgggcct tccaacgcgg
    30601 tttcgttcgg atgaatcacg ccttccaata caatttcagc acgggcaggc acttgcaaat
    30661 cgttgccgat acatttcacc agctccgtcc gcgaaccgcg cagcagtccg gcaaactggt
    30721 attcgctcaa agtatcggga acaggcgtta ccgctccgag aatagtggca gggtcgcaac
    30781 cgagcacgac ggcgacggga tacggcgtat cgggattgag tttgcggaac tcctgataat
    30841 ccaacgcgcc gccgcgatgc gacagccaac gcataatcag cttgtttttg ccgatgagtt
    30901 gttggcggta aatgccgaga ttttggcgtt ttttgtgcgg cccgcgcgtt acggtcaagc
    30961 cccatgttac cagcggcgca acgtcttccg gccagcaatg ctgaatcgga agtttatata
    31021 aatcaacgtc ttcgccttcc cacacgattt cctgacacgg cgcgtttttc accacgttcg
    31081 gcgccatgct ccaaatgtct ttcaagagcg gcagtttgga aaacgcgtct ttgatgcctt
    31141 tgggcggttc gggttctttc agatacgcca gcgtctgccc gatttcgcgc agcttggaca
    31201 cgctgtccgc gcccatgccc atcgccacac gttcgggcgt gccaaacaaa ttcgccaaca
    31261 caggataatc gtagcgcgta ccgtcgggct taatcgggtt ttcaaacaac aacgccggcc
    31321 cttcggcgcg caacacgcgg tcggcgattt cggtcatttc caaatgcggg gaaacaggat
    31381 gcgcgacgcg cttgagtttg ccctgttgct cgagcatagc gatgaagtcg cgcaggtctt
    31441 tgtatttcat attcatcctt tttgtccttt tatcctgagc aatccgattc ggataccgcc
    31501 cctatccttg cctgcgcttc ggcatattct atgccgtgat aaaagtcgcg taccagcgga
    31561 tgtccgccac tttgatgaag ttgcagcaaa gggcgttggt tgtcggactg ggtaacgaca
    31621 ttgcagtgca gaccgaaggt gtcggtttca taagggggca gccagttgca gatcatgccg
    31681 aaataaacga cgttttccgg attgtcgtga aagtggcttt gatagtcgtt aaaactcttt
    31741 tcactgacag atacccatac cccatattcc agcgtttcct catgcccgat aatcggaata
    31801 agcagcaccg cgcggataaa acggtcggtt tggtcgggat agcggatgat gcagaaatca
    31861 gaatcacatt ccgcttgata agcaatgcgt tcttcttcac tgagttgatt atagggatag
    31921 ggggcggtaa agccgattgc gggcatatct tcatgttttt cgccgcagga agtgcaagtg
    31981 tacataaggt ttcagacttt caaaacgagt ttgcggtaaa gccattcgcc ggcaaacggc
    32041 atccccatca atacataggc aatcacgccg gtataaaccg cccaccaatc atatcgcccc
    32101 aaccgtgcca acaaagcggc aagcgtcccg ttgattataa aaaatacgca ccaaacctgc
    32161 gttacccggc gggtatagcg cacggctttt tcaggcaggt cgggctgttg cagccgcgca
    32221 agtttttcaa tcaccgtctg cccggcaaac aggcttcccc cgaacaccgc caacatcatc
    32281 agattgacga ggacgggata ccaatacatt gagtcgcgcc gcccgaacac caatactgcg
    32341 gcaaaaaaca gtgtaataaa caaagccgca taacgctgtt ggggcaattt ggcagtcagg
    32401 gcgcgcagca gccacaaccc gcacatcgcc gccgccagcc agacaaacca gcccgcctcc
    32461 ctgccgtaat gatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact
    32521 atctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataccac aaagcgggat
    32581 aggcaatgct taatacggtc agaaaaatat gtccgaaaaa accgggtttc attttgaatc
    32641 cgcacaaatg ttttcagacg gcatccgata aaaacatgcc gtctgaaaaa taattagaaa
    32701 tacccgatta gcccgcctga atcttcaata ccgcctgtac cacgtcgttg acggtgcgga
    32761 cattgcggaa atcttcagcc tgcagcttgc gggctgccac agccattcac gattgcgata
    32821 tacccggcgg cattcccgac tgccgttcag cagaaagcgc acccattcca aaccgctcca
    32881 atatgcctcg ggcagcatac cggcttcaga cggcatatcg tccgaagcag ataaagtcag
    32941 gctgtaacgc gtccctttgg tcagaaccat cgccaaagca taagcaaacg gcgcgcgcgt
    33001 tgccgatacg gcatatcctt ccggcagcgg atcgtccgcc gccaaaacca aaaccgaccc
    33061 gcatccctct tccaacagtg atgccgcttc cgccaatgcc gtttccacac cgtcggcaca
    33121 cacggccaat gccgtctgct cgctcatatc ccgcctcaat atcgaccatt accccgccgt
    33181 tgcattgtgc accgacaaac cgaacgaagt cggcgacacg gtttgcgatt tcaacagttc
    33241 caaccacaaa tcgaaactgc gcgccatttc cccgtcgtgc gaggcataaa ctatagtgga
    33301 ttaaatttta ggggctgtac tagattagca gatatgttac cctcgaaata tgaagataac
    33361 acactgcaaa ttaaagaaaa aagtacagaa agaactgctc cgttttttgt gctggaagtt
    33421 accgcccgtt ctgccgccga tattttgggt atccatccca attcggcagc actgttctac
    33481 cgtaaaatcc gcacggttat caaccatcat ttggccttgg ctgccgatga ggtttttgag
    33541 ggccctgtcg agccggacga aagcgatttc ggcggacggc gtaaaggtag acgtggtcgc
    33601 gggcggcagg aaaagtggtt gtcttcggca ttctgaaacg caacggacgg gtctataccg
    33661 ttgtcgtaaa caatgccaag tctgaaacgt tactccctgt catcaaaaag aaaatcatgc
    33721 cggacagtat tgtttatacc gatagtctga gcagctgcga caagttggac gtgagcggtt
    33781 ttatccatta ccgcatcaac cattccaagg aatttgcaga ccgtcaaaac cacattaacg
    33841 gcattgagaa cttttggaat caggcaaaac gcgtcttgcg aaaatatagt ggattggcac
    33901 gaacgtttcc ccgaattgga agcgcaactg ggtgcgccga ttgaggatta cgcgccgccg
    33961 aaacattgga cgcgcaagca gctcagaagt atggggcgtg tgtcgtattt gtgcgtcgat
    34021 gcggcggagc aggctttgac ggatgccggt ttgctcggag acgaaagcat taccgacgga
    34081 cgcatgggcg ttgcctgcgg ctcttccagc ggcagcacca aagacatcgg cgatgtaggc
    34141 gaattgttgc tgaccggcat gtcgcgcaac ttcagtgcca acacctatgt gcgtatgatg
    34201 ccgcacaccg ccgccgccaa tatcggcatc tttttcgggc tgaaaggacg catcatcccg
    34261 acatcgagcg cgtgttcgtc cggcagccaa ggcataggtt atgcctacga agccatcaaa
    34321 tacggtctga ccgatatgat gctggcgggc ggaggcgaag aatttttccc gtccgaagtg
    34381 tatgttttcg actcgcttta tgccgccagc cgccgcaacg gcgaaccgga aaaaaccccg
    34441 cgcccatacg acgcgaaccg cgacgggctg gtcatcggcg aaggcgcggg gattttcgtg
    34501 ctggaagaat tggaacacgc caaacggcgc ggtgcgataa tttacgccga actcgtcggc
    34561 tacggagcca acagcgatgc ctaccatatt tccacgcccc gccccgacgc gcaaggcgca
    34621 atccttgcct ttcagacggc attgcaacac gcaggtcttg cgcccgaaga catcggctgg
    34681 attaatctgc acggcaccgg gacgcaccac aacgacagta tggaaagccg tgccgttgca
    34741 gcggttttcg gcaacaatac gccctgcacg tccaccaagc cgcaaaccgg acacacgctg
    34801 ggtgcggcgg gcgcaatcga agccgcgttc gcgtggggca ttgccgaccg gcaaagcaat
    34861 cccgaaggaa aacttccgcc ccagctttgg gacgggcaga acgaccccga cctgcccgcc
    34921 atcaacctga ccggcagcgg cagccgctgg gaaaccgaaa aacgcattgc cgccagctcg
    34981 tcgtttgcct tcgggggaag caactgcgtc ttaatcatcg gatgaaataa gtttgtcaat
    35041 cccaccgcta tgctatacaa tacgcgccta ctcttgacgg gtctgtagct caggggttag
    35101 agcaggggac tcataatccc ttggtcgtgg gttcgaaccc caccggaccc accaattccc
    35161 aagcccggac gtatgtttgg gcttttttgc cgccctgtga aaccaaaatg ctttgagaaa
    35221 ccttgataat gaaaaaagtc agcgtattga ttgttgccaa aaacgaagca aaccacattc
    35281 aggaatgtat tgaaagttgc cgtttcgata aagaagtcat cgttatcgac gactacagca
    35341 ccgacaatac tgccgaaatt gccgagggtt tgggcgcaaa agtcttcaga cggcatttga
    35401 atggggattt cggagcgcaa aaaacatttg ccatcgaaca ggcaggcgga gaatgggttt
    35461 tcctgattga tgcagacgaa cgctgcacgc cggaactatc tgatgaaatc tcaaaaattg
    35521 tccaaaccgg cgattatgcc gcctattttg tcgaacgccg caaccttttc cccaaccatc
    35581 ccgccacaca cggcgcgatg cgtcccgaca gcgtatgccg tctgatgccg aaaaaagaca
    35641 gttcggtgca aggcaaagta cacgaaaccg tacaaacccc ctaccccaaa cgccgtctga
    35701 agcattttat gtaccattac acgtacgaca actgggaaca atatttcaac aagttcaaca
    35761 aatatacttc catttcagcc gaaaaatacc gagagcaggg aaagcccgtg cgtttcgtta
    35821 gggacattat cctccgcccg atttgggggt ttttcaaaat ttatatcctg aacaaagggt
    35881 ttctcgatgg aaaaatgggt tggattatgt ccgtcaacca cagctattac acgatgatta
    35941 aatatgtcaa actatattat ctgtacaaat ctggcggaaa attttaaatg gaaaaagaat
    36001 tcaggatatt aaatatcgta tcggccaaga tttggggtgg gggcgaacaa tatgtctatg
    36061 atgtttcaaa agcattgggg cttcggggct gcacaatgtt taccgccgtc aataaaaata
    36121 atgagttgat gcacagacgc ttttccgaag tttcttccgt tttcacgaca cgccttcaca
    36181 cgctcaacgg gctgttttcg ctctgcgcgc ttacccgctt tatccggaaa aaccgcattt
    36241 cccacctgat gatacacacc ggcaaaattg ccgccttatc catacttttg aaaaaactga
    36301 ccggggtgcg cctgatattt gtcaaacata atgtcgtcgc caacaaaacc gatttttacc
    36361 accgcctgat acagaaaaac acagaccgct ttatttgcgt ttcccgtctg gtttacgatg
    36421 tgcaaaccgc cgacaatccc tttaaagcaa aataccggat tgttcataac ggtatcgata
    36481 ccggccgttt ccctccctct caagaaaaac ccgacagccg tttttttacc gtcgcctacg
    36541 ccggcaggat cagtccagaa aaaggattgg aaaacctgat tgaagcctgt gtgatactgc
    36601 atcggaaata tcctcaaatc aggctcaaat tggcagggga cggacatccg gattatatgt
    36661 gccgcctgaa gcgggacgta tctgcttcag gagcagaacc atttgtttct tttgaagggt
    36721 ttaccgaaaa acttgcttcg ttttaccgcc aaagcgatgt cgtggttttg cccagcctcg
    36781 tcccggaggc attcggtttg tcattatgcg aggcgatgta ctgccgaacg gcggtgattt
    36841 ccaatacttt gggcgcgcaa aaggaaatta tcgaacatca tcaatcgggg attctactgg
    36901 acaggcttac gcctgaatct ttggcggacg aaatcgaacg cctcgtctta aaccccgaag
    36961 caaaaaacgc actggcaacg gcaggacatc aatgcgtcgc caaccgtttt accatcaacc
    37021 ataccgccga caaattattg gatgcaatat aaactgcttt cagacggcct atgccgtctg
    37081 aaagcctttg atgcaacaaa ccgccaaatt atattcgttc attggaaaga aacaccccga
    37141 attcatcctt caaaataaga aaatcccaat atcccccgat attacgcagc ctattggcaa
    37201 agttttgcag cgtcttcccc ggcttgtgcc gccgcgtcaa gtgctttgtt acaatgcact
    37261 accttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
    37321 tttcgtactt gctgcagcag gttcggccat cggtttgggc gcgatttgga aatttcctta
    37381 tacggcaggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttactatctt
    37441 ggtcgcccta cccgttcagc ttgccgaatt ttatatcggg cgcacgggcg gtaaaaatgc
    37501 cgtcgattcc ttcagggttc tgcgtccggg cacgcaatgg ctttgggtcg ggcgtatggg
    37561 cgttgccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
    37621 ttatgtcgtc cacagtttta cgggggcggt tcataccggc gcggactttg aagccttgtt
    37681 cggcgcgacg atttccaatc cggcaggttc gctgtcctat caggctctgt ttatgctgat
    37741 tacggtttgg gtggtcaaag gtgggatttc agacggcatt gaaaaggcaa accgttatct
    37801 gatgccgggg ctgtttatcc tctttattgc gctggcaatc cgttcgctga cgctgccggg
    37861 tgcaatggag ggcgtgtcat tcctgctcaa accgaattgg tcgtacttta aagccgatac
    37921 gatgattacg gctttgggac aggcgttttt tgccctgagc atcggtgttt ccgccatgat
    37981 tacctacgct tcatatttgg gaaaagatca ggatatgttc cgctcggggc atacgattat
    38041 gtggatgaac ctcttggttt cgctgcttgc cggcctggtg atttttccgg cggtgttcgc
    38101 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc ccgcagtgtt
    38161 tatgaagatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
    38221 cacgctgact tccgcatttt cgatgttgga aacggtcatc gccgcaacca tccgccaaga
    38281 cgagcgcaaa cgcaaaaaac acacttggct tatcggcacg gccattttca ttatcggcat
    38341 cccatccgcg ctgtctttcg gcgtatgggg tgagtttaag gttttcggca aaaccatttt
    38401 tgatttgtgg gactatgtta tttccgccgt cattatgccg attggtgctt tgagtgtttc
    38461 catctttacc gcctggattc aggacaagca gtctgtgtta aaagatgccg gcgcgggcag
    38521 caccgtacca cgggcagtgc tgctgctgtg gctgaatacc ttgcgctacc ttgccccgat
    38581 tgccattatt attgttttca tcaattcttt ggacatcctt taaaagccat ccaaacagca
    38641 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt caggttcagc ctatttcgtt
    38701 caaagtatag tggattaaca aaaatcagga caaggcgacg aagctgcaga ccgtacagat
    38761 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
    38821 ggcgaggcaa caccgtactg gtttttgtta atccactata gccttgcgcg atgccgttca
    38881 aggacaaacc catacccttt tcggcaaaac ggatttcacg gtcgtcaaac gagactttgc
    38941 cgaagccgac ccgtttcagg gcttccttca cgctgttttg aggcggcggc gtttccgcat
    39001 cgggacgggc ggcaaaataa tcggcataca gtttccacaa cgcctgcact gtcggatcga
    39061 acgcgccgtc cgtcagcgcg tgaatatcgc ggcacaggct caacagttcc aaaaaatccg
    39121 ccgacggcga agtcagataa ccgtccctgt tcaggcggct gatcaggctg tcttcacggt
    39181 aaaggctgaa caattttttc caaacgcgcc acttccgcca aaaccttgtt gaccaaatcc
    39241 gccgcacgcc tgtcgtccac accgaacaga cggagctccg caccggaacc cagtgcgaca
    39301 cctttccaga aaaacacatt ttcactgcgt ttttcatccc cgttgcgttt ttcatcatcg
    39361 gcggcaaaag gattcggcaa aaaagaaacc gccgcgcccg ccgccgcaac ggcggcagcc
    39421 gtcaaaaaac gcctgcgccc gaaatgcctg cccataccgc ctcctaaacc gacactgccg
    39481 ccttgatgtg cggatgcgga tcgtagcctt ccaactcgaa atcttcaaac ttgaaggaaa
    39541 acaaatcttt gacttcagga ttgattttca tcactggcaa ggcgcgcggt tcgcgttcca
    39601 actgcaatgc ggcctgctcg aaatggttgc ggtacaaatg cgcgtcgcca aacgtatgga
    39661 caaactcgcc cgcctccagc ccgcacactt gcgccatcat catcgtcaac agcgcgtagc
    39721 tggcaatatt aaacggcaca ccaaggaaaa tatctgcact acgctggtaa agctggcagg
    39781 acagtttgcc gtcggcaacg taaaactgaa acagcgtgtg gcagggcggc aaggccattt
    39841 catcgaccaa agccggattc cacgccgata caatcaggcg gcgcgagtcg ggattcttct
    39901 tgatttgttc cagcacattg gcgatttggt cgatatgcct gccgtcgggc gcgggccagt
    39961 tacgccactg gtagccgtaa accgggccta agtcgccgtt ttcgtccgcc cactcgtccc
    40021 aaatggaaac attgttgtcc tttaggtatt tgatattggt atcgcctttg agaaaccaaa
    40081 gcagctcgtg gataatcgaa cgcagatgca gctttttggt cgtcagcagc ggaaaacctt
    40141 tgcccaagtc aaaacgcatc tgataaccga atacggagcg cgtacccgta ccggtgcggt
    40201 ctgatttgtc cgtaccgttg tcgaggacgt ggcgcatcaa gtccaaatag gctttcatag
    40261 cagtctttca tcaaattaaa cggcgcatat tgtaacattt ccggataatg cccaaaacac
    40321 ggatacaggc aggcaggatt gttggcaatt tcagtccttt tccacagtaa aacccggtgg
    40381 gaaaacaaaa ttaccttgat tggaatcaaa aaatctagtt taattactta gaataaaatt
    40441 tcaataatat ttttatttac gaaattaatt tatgattatt gattaattat cggcaacaaa
    40501 catcaaacat cgaaaacatg gaaaaaataa tgtcaacaat ttttgccaaa tcgggcttgg
    40561 catcagaaaa aaataggttt atattcccac ctacaaattt gttttcccat tagtacacta
    40621 tcaaccaaaa ggagtatccg aatgactgac ctgaacaccc tgtttgccaa cctcaaacaa
    40681 cgcaatccca atcaggagcc gttccatcag gcggttgaag aagtcttcat gagtctcgat
    40741 ccgtttttgg caaaaaatcc gaaatacacc cagcaaagcc tgctggaacg catcgtcgaa
    40801 cccgaacgcg tcgtgatgtt ccgcgtaacc tggcaggacg ataaagggca agtccaagtc
    40861 aaccggggct accgcgtgca aatgagttcc gccatcggtc cttacaaagg cggcctgcgc
    40921 ttccatccga ccgtcgattt gggcgtattg aaattcctcg cttttgaaca agtgttcaaa
    40981 aacgccttga ccaccctgcc tatgggcggc ggcaaaggcg gttccgactt cgaccccaaa
    41041 ggcaaatccg atgccgaagt aatgcgcttc tgccaagcct ttatgaccga actctaccgc
    41101 cacatcggcg cggacaccga tgttccggcc ggcgacatcg gcgtaggcgg gcgcgaaatc
    41161 ggctacctgt tcggacaata caaaaaaatc cgcaacgagt tttcttccgt cctaaccggc
    41221 aaaggtttgg aatggggcgg cagcctcatc cgtcccgaag cgaccggcta cggctgcgtc
    41281 tatttcgccc aagcgatgct gcaaacccgc aacgatagtt ttgaaggcaa acgcgtcctg
    41341 atttccggtt ccggcaacgt ggcgcaatac gccgccgaaa aagccatcca actgggtgcg
    41401 aaagtactga ccgtttccga ctccaacggc ttcgtcctct tccccgacag cggtatgacc
    41461 gaagcgcaac tcgccgcctt gatcgaattg aaagaagtcc gccgcgaacg cgttgccacc
    41521 tacgccaaag agcaaggtct gcaatacttt gaaaaacaaa aaccgtgggg cgtcgccgcc
    41581 gaaatcgccc tgccctgcgc gacccagaac gaattggacg aagaagccgc caaaaccctg
    41641 ttggcaaacg gctgctacgt cgttgccgaa ggtgcgaata tgccgtcgac tttgggcgcg
    41701 gtcgagcaat ttatcaaagc cggcatcctc tacgccccgg gaaaagcctc caatgccggc
    41761 ggcgtagcaa cttcaggttt ggaaatgagc caaaacgcca tccgcctgtc ttggactcgt
    41821 gaagaagtcg accaacgcct gttcggcatc atgcaaagca tccacgaatc ctgtctgaaa
    41881 tacggcaaag tcggcgacac agtaaactac gtcaatggtg cgaacattgc cggtttcgtc
    41941 aaagttgccg atgcgatgct ggcgcaaggc ttctaagcaa acgccgccgc tccgcaaaca
    42001 aaatgccgtc cgaaccgcaa atgctgttca gacggcattt cattatccgc ccgttcgaat
    42061 cgggtgatac tatttgtatc cagaattgtt ggatagtttg ggagtggaga ggaagaaatg
    42121 attatggacc aatactatat ggagttaaag aataagttat ctaacagacc aatcctactg
    42181 gataatacta atgattttct ttttgtttta gtaaatacag tgaaagctat gatagaaaat
    42241 actgataaaa gtcagctttc agagttagat aaaattttag acggtgtcac aagtcaagaa
    42301 ttgaaacttg cctatgattt ttgtcaaggg aaattcggtc aagcaggttt ttcttatcgt
    42361 aggcacccga attattttta tttgtccagt ttaattgcga cttttccaga atttgagttg
    42421 tctaaagcag accgagatta cctcaaagga atcataaatt ttgataacta ccttttatat
    42481 gagttagatt agtctttaat cacctcaaaa ccctatttat tcaaaaggag aaaaaatgat
    42541 tgagctgaaa ttagtagatg agagcagttt tcaggcagtg ctggatttga aaatatctga
    42601 agctgatgaa cgagcacgtt ttgtggctcc aaatgtacgc tctttagctg acgcatggct
    42661 ctaccgggaa aatgaagatg tgtttccgag ggcaatctat tgggataagc aagtggttgg
    42721 ctttctcctg ctggaaatag acaaggatga agcagaatac tttatctggc ggataatgat
    42781 tggtcagcag tg
//
LOCUS       DAWFIT010000005        42534 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFIT010000005 DAWFIT010000000
VERSION     DAWFIT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.005"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WGQ85_000240"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WGQ85_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0749749.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2480..2570)
                     /locus_tag="WGQ85_000241"
     tRNA            complement(2480..2570)
                     /locus_tag="WGQ85_000241"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Ser,seq:gga)
     gene            complement(2609..2695)
                     /locus_tag="WGQ85_000242"
     tRNA            complement(2609..2695)
                     /locus_tag="WGQ85_000242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2659..2661),aa:Leu,seq:cag)
     gene            complement(2731..2817)
                     /locus_tag="WGQ85_000243"
     tRNA            complement(2731..2817)
                     /locus_tag="WGQ85_000243"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2781..2783),aa:Leu,seq:cag)
     gene            2906..5281
                     /gene="rnr"
                     /locus_tag="WGQ85_000244"
     CDS             2906..5281
                     /gene="rnr"
                     /locus_tag="WGQ85_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0749750.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERRMRGIMHGDIVTVRPAGMDRRGRREAVVLDIVERAQ
                     SKVVGRFYMDRGVAVLEAEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCSADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGQ85_000245"
     CDS             complement(5620..7083)
                     /gene="guaB"
                     /locus_tag="WGQ85_000245"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0749751.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVAGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGQ85_000246"
     CDS             complement(7223..9781)
                     /gene="glnD"
                     /locus_tag="WGQ85_000246"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0749752.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLNNPKIQSSLKQTLLEQLS"
     gene            complement(9829..10149)
                     /locus_tag="WGQ85_000247"
     CDS             complement(9829..10149)
                     /locus_tag="WGQ85_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0749753.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGQ85_000248"
     CDS             complement(10474..10947)
                     /gene="bfr"
                     /locus_tag="WGQ85_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0749754.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGQ85_000249"
     CDS             complement(10975..11439)
                     /gene="bfr"
                     /locus_tag="WGQ85_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0749755.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGQ85_000250"
     CDS             complement(11902..12885)
                     /gene="lipA"
                     /locus_tag="WGQ85_000250"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0749756.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGQ85_000251"
     CDS             complement(12878..13501)
                     /gene="lipB"
                     /locus_tag="WGQ85_000251"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0749757.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGTEMTQIADFVQPCPTPDEVAAKLTAHLATQFTPKADNNE"
     gene            complement(13503..13778)
                     /locus_tag="WGQ85_000252"
     CDS             complement(13503..13778)
                     /locus_tag="WGQ85_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0749758.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            13963..15033
                     /locus_tag="WGQ85_000253"
     CDS             13963..15033
                     /locus_tag="WGQ85_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0749759.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFTGSFYRGR"
     gene            complement(15140..16087)
                     /locus_tag="WGQ85_000254"
     CDS             complement(15140..16087)
                     /locus_tag="WGQ85_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0749760.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(16102..16509)
                     /locus_tag="WGQ85_000255"
     CDS             complement(16102..16509)
                     /locus_tag="WGQ85_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0749761.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIIYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            16590..17249
                     /gene="ung"
                     /locus_tag="WGQ85_000256"
     CDS             16590..17249
                     /gene="ung"
                     /locus_tag="WGQ85_000256"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0749762.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            17246..17455
                     /locus_tag="WGQ85_000257"
     CDS             17246..17455
                     /locus_tag="WGQ85_000257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0749763.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            17352..17999
                     /locus_tag="WGQ85_000258"
     CDS             17352..17999
                     /locus_tag="WGQ85_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0749764.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            18072..18545
                     /locus_tag="WGQ85_000259"
     CDS             18072..18545
                     /locus_tag="WGQ85_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0749765.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTSTNLVTAVWCI"
     gene            18533..18847
                     /locus_tag="WGQ85_000260"
     CDS             18533..18847
                     /locus_tag="WGQ85_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ0749766.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            18889..19833
                     /locus_tag="WGQ85_000261"
     CDS             18889..19833
                     /locus_tag="WGQ85_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0749767.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            19975..20109
                     /locus_tag="WGQ85_000262"
     CDS             19975..20109
                     /locus_tag="WGQ85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0749768.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(20399..20914)
                     /locus_tag="WGQ85_000263"
     CDS             complement(20399..20914)
                     /locus_tag="WGQ85_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749769.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(21380..23299)
                     /locus_tag="WGQ85_000264"
     CDS             complement(21380..23299)
                     /locus_tag="WGQ85_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0749770.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(23376..23771)
                     /locus_tag="WGQ85_000265"
     CDS             complement(23376..23771)
                     /locus_tag="WGQ85_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0749771.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            23944..25251
                     /locus_tag="WGQ85_000266"
     CDS             23944..25251
                     /locus_tag="WGQ85_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0749772.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            25244..25678
                     /locus_tag="WGQ85_000267"
     CDS             25244..25678
                     /locus_tag="WGQ85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749773.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(25737..26006)
                     /locus_tag="WGQ85_000268"
     CDS             complement(25737..26006)
                     /locus_tag="WGQ85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0749774.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGQ85_000269"
     CDS             complement(26188..28650)
                     /gene="lon"
                     /locus_tag="WGQ85_000269"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0749775.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            28735..28926
                     /locus_tag="WGQ85_000270"
     CDS             28735..28926
                     /locus_tag="WGQ85_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0749776.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            28901..30004
                     /locus_tag="WGQ85_000271"
     CDS             28901..30004
                     /locus_tag="WGQ85_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0749777.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGQ85_000272"
     CDS             complement(30076..31821)
                     /gene="recD"
                     /locus_tag="WGQ85_000272"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0749778.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGKDENLPNIALAAPTGKAAAHMARALHRAINGFDALEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGQ85_000273"
     CDS             complement(31889..32584)
                     /gene="lolD"
                     /locus_tag="WGQ85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0749779.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(32577..33824)
                     /locus_tag="WGQ85_000274"
     CDS             complement(32577..33824)
                     /locus_tag="WGQ85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0749780.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            34030..34308
                     /locus_tag="WGQ85_000275"
     CDS             34030..34308
                     /locus_tag="WGQ85_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749781.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGQ85_000276"
     CDS             complement(34364..34981)
                     /gene="recR"
                     /locus_tag="WGQ85_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0749782.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(35048..36586)
                     /locus_tag="WGQ85_000277"
     CDS             complement(35048..36586)
                     /locus_tag="WGQ85_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0749783.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(36640..37014)
                     /locus_tag="WGQ85_000278"
     CDS             complement(36640..37014)
                     /locus_tag="WGQ85_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0749784.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            37164..38792
                     /locus_tag="WGQ85_000279"
     CDS             37164..38792
                     /locus_tag="WGQ85_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0749785.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            38864..40117
                     /locus_tag="WGQ85_000280"
     CDS             38864..40117
                     /locus_tag="WGQ85_000280"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0749786.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            40340..40648
                     /locus_tag="WGQ85_000281"
     CDS             40340..40648
                     /locus_tag="WGQ85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749787.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            40730..41761
                     /gene="ruvB"
                     /locus_tag="WGQ85_000282"
     CDS             40730..41761
                     /gene="ruvB"
                     /locus_tag="WGQ85_000282"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0749788.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGQ85_000283"
     CDS             complement(41784..42512)
                     /gene="rpe"
                     /locus_tag="WGQ85_000283"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0749789.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
BASE COUNT         9698 a        11162 c        11400 g        10274 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gacaagccgt ccgctcgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacga atcaaacccg gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggta tcggcgtaac
      961 catcaccgac aaagacaatc ccaaaggcct accgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagatacc gccgatgccg acgtgttccg cgtatccggg cgcggcgagc tgcacctgac
     1201 cattttgctg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccgtat gaaaacctga ccgtggatgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 tgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 tcgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga agaggatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     2221 atagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     2281 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaaatatacc
     2341 gcaaaacata ccataaaaaa aacggtaacg gatagttgta agcggttgat gacgattata
     2401 gacaataacg ggttttccca atgaaattat tgtttgaata ataaaaaatc ccaaaccgta
     2461 aaagtttggg atttgtattt ggcagagagg aagggattcg aaccctcgat acgctattca
     2521 cgtatacacg ctttccaggc gtgcgactta aaccactcat ccacctctct aatggcggaa
     2581 attatcccaa tcgggataat ttattatttg gtgcccggga gaagactcga acttccacac
     2641 ccatgaggat accagcacct gaagctggcg cgtctaccaa ttccgccacc cgggcaatct
     2701 aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg gagaagactc gaacttccac
     2761 acccgtgagg atactagcac ctgaagctag cgcgtctacc aattccgcca cccgggcttt
     2821 gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa gaagtcgcta ttatatagtt
     2881 cataaagaga atgtcaacag tccaaatgaa taaaaatatt aaatctttaa atttacggga
     2941 aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa catcctttgc ccagtcggga
     3001 atggataatc gaattgttgg agcgcaaagg tgtgccttca aaaatcgaat cgcttgcgcg
     3061 cgagctgtcg attacggaag acgagtatgt ctttttcgaa cgccgtctga aggcgatggc
     3121 gcgggacggt caggttttaa tcaaccgccg gggcgcggtt tgcgcggcgg acaaattgga
     3181 tttggtcaaa tgccgtgtcg aggcgcacaa agacggcttc ggttttgccg tgccgctcac
     3241 gcccgccaaa gacggtgatt ttgttttgta cgaacgccgg atgcgcggca ttatgcacgg
     3301 cgatattgtc actgttcgtc ctgccggcat ggaccgcagg gggcggcggg aagcggtggt
     3361 tttggatatt gtcgaacgcg cgcaaagcaa agtggtcggg cgtttctata tggatcgcgg
     3421 cgtggcggtt ttggaagcgg aagacaagcg tctgaaccaa agcatcgtat tggaaccgga
     3481 cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc gtcggcgaaa ttgaggttta
     3541 tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa gttttgggcg attatgccga
     3601 cagcggcatg gagattgaaa ttgccgtgcg caagcatcat ttgccgcacc aattcagtga
     3661 agcgtgtgcc aaagccgcga aaaaaattcc cgaccatgta cgcaaaagcg atttgaaagg
     3721 ccgcgtcgat ttgcgcgatt tgcctttggt aacgatagac ggcgaaacgg cgcgcgattt
     3781 tgacgatgcc gtgtttgccg agaaaatcgg acgcaattac cgtctggtcg tggcgattgc
     3841 cgatgtcagc cattatgtcc gccccgatga cgctatcgac acggacgctc aggaacgcag
     3901 caccagtgtt tacttcccgc gccgcgtgat tcccatgttg ccggaaaacc tgtccaacgg
     3961 catctgctcg ctcaatccta atgtcgagcg tttgtgtatg gtgtgcgata tggtcatcac
     4021 ttacgcgggc aatattaaag aataccgctt ctaccccgcc gtgatgcgct ctcatgcccg
     4081 cctgacctac aaccaagttt ggaaatggct ttcaggcggc atcgggcatc cgttcaaagc
     4141 ccaaatcgac acgctttaca aactcttcaa aatccttcag aaaaagcgtt tcgaacgcgg
     4201 ggcggtggag tttgacagca tcgaaaccca aatgcttttc gacgacaacg gtaaaattga
     4261 aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg attgaagaat gtatgctggc
     4321 ggcgaatgtt tgctcagcgg attttctatt gaaaaataag catacggctt tgtttcgcaa
     4381 ccatttgggc cccacgcccg aaaaactcgc caccctgcgc gagcagctcg gtctgttggg
     4441 gcttcaactt ggcggcggcg acaacccgtc gccgaaagac tatgccgcgc ttgtcgaaca
     4501 attcaaaggc agacctgatg ccgaattgct gcaagtcatg atgttgcgct ccatgcagca
     4561 ggcggtttac gaaccgcatt gcgacggaca ctttggtctt gcctacgaag catacgccca
     4621 cttcacctcg cccatccgcc gctatcccga cctgaccgta caccgcgcca tcaaagccgt
     4681 gttgaatcag caaacctaca cgccaaaaaa aagctggcag gctttgggcg tgcatacctc
     4741 gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg gaaaactggc tgaaaaccta
     4801 ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa atctccggca tgaccagttt
     4861 tggtatcttt gtaacactgg acggcatcca cattgacggc ttggtgcata tcagcgattt
     4921 gggcgaagac tatttcaact tccgccccga aatcatggca atcgaaggcg aacgcagcgg
     4981 catccgtttc aacatggggg acagggttgc cgtccgggtc gcccgtgccg atttggatga
     5041 tggaaaaatc gattttgtcc tgattgccga ggggagcggc agggggcgga aagttaaatc
     5101 atccgcgtct gccaaaccgg cagggacggc ggggaaaggg aaaccgaaaa ccgccgccga
     5161 gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc gcgtctgccg ccgcagaatc
     5221 ggggaaaaag gcaaagaaac cggttccgat taaggtaaaa aaacggaaag gcaaatcata
     5281 atgctgacgg ggtggcttgg ggagggcggg gcatactttc ccgaagccgc cgcccgattt
     5341 tttgatatgg cggccggcgc atcatttcag gccaatcgaa acgcccgtat tgaaagattg
     5401 cgtttatttc caccgccgtt ttaaaggccg acggtattcg gcagacgggg cgcaaacggc
     5461 gttcagacgg cattttcatt ctttcggcgt gtccgtccga attgctttgc ccgtccgcgc
     5521 aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc cgtctgaaac ccgaaaatca
     5581 ggtttcagac ggcatttcgc cttggaaagg ctgttcaaat cagcggtggt aattcggtgc
     5641 ttctttggta atttgaacgt cgtgaacgtg cgattcgctc atacctgcgg aagtgatttc
     5701 cacaaattcg gctttttcgt gcatttcggc aatattggcg caacccaaat aacccatact
     5761 ggaacgcaaa ccgccggtca gttggtggat gatgttcaca atcggacctt tgtaaggaac
     5821 gcggccttcg atgccttcgg ggacgtattt gtcggtgctg tcggttttgt cttggaagta
     5881 gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc ataccgcgat aggatttgta
     5941 cgagcggcct tggtagagtt cgatttcgcc cggtgcttct tccgtgccgg caaacatacc
     6001 gccgagcatg acgctgtacg cgcctgcggc gagggctttg gcgatgtcgc cggagaagcg
     6061 gatgccgccg tcggcaatca gcggaacgcc cgtgcctttg agggcttcgg caacgttgtg
     6121 aatggcggtc agttgcggca cgcccacgcc tgccacgata cgggtggtgc aaatcgaacc
     6181 cggaccgata ccgactttga cggcatccgc gccggcggca accaaatcca aagcggcttt
     6241 ggcagtggcg atattgccgc cgatgacttg gatgtgcgga taagtttctt tgacccaacg
     6301 cacgcgatcg attacgcctt ggctgtgtcc gtgtgcggta tcgacgacaa ttacgtccgc
     6361 gcctgcttcg accaaggctt tgacgcgttc ttcggtatcg ccgccggtgc cgactgccgc
     6421 accgacgcgc agacggcctt cggagtcttt gttggcattg ggaaattcgg tggtttttaa
     6481 aatgtcttta acggtaatca agcctttaag ctcgtctttt tcgttcaaga ctaaaacgcg
     6541 ctcgacttta tgcgtgtgca tcagttcgcg cgcttcgtct atgcttgtgc cttcggggac
     6601 ggtaaccaag cgttcgcgcg gggtcataat ggcggaaacg ggcaaatcga cgcggttttc
     6661 aaaacgcagg tcgcggttgg ttacgatgcc ggcgactttg ccgttttcaa cgacgggcag
     6721 gccggacatt ttgcgtttgc gctgcgcgcg catttccaag acttcgcgga tgagcgttgt
     6781 cggcgcaacg gttacggggt ctttgaccac gccgctttcg tggcgtttca ctttggaaac
     6841 ggcgcgagcc tgcatctccg gcggcatatt tttatggatg atgccgatgc cgccttcctg
     6901 tgccatcgaa atggcgaggc gcgcttcggt aacggtgtcc atcgcggcgg aaagcagggg
     6961 gaggttgagc gtgatttcgc gggtgagctt ggtttgaagt ttaacgtctc gcggcagcac
     7021 ggttgagtgt gcgggaacca acaaaacatc gtcgaaagta taggcttttt ctacgatacg
     7081 cataatgctc ggtctttcag tttgtgcaag atgcacggca ttatagcacg ttgccggcgg
     7141 cttgacagtt tatattgttt gcaatatccc cgccttcagg cggcatacgg cgcggcggcg
     7201 gaatgccgtc tgaaggcggg cgttatgata attgttccag cagcgtctgc ttcaatgagg
     7261 actggatttt ggggttgttc aaatcggggc tgaaaacggt aaaactgtct tcggcgcgtt
     7321 cgtccaacgt ggagattttg gcgtagcgca ggctgacgtt gtgggcgaaa aagacttccg
     7381 ccatatcggc gagcaggaag gggcggttga cggcggtgat ttcgacggaa taccagtcgg
     7441 gatagtcttc ttcgggggtg atggtgatgc tcggtgcgat cggcatatag cggctgcggc
     7501 ggctgatgcg gcggctgcgg ctttgggttt cggcaacggt gtgtccgtgg ataaagctgt
     7561 tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggggcg tgctgcgagg
     7621 ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgatgaag gcgcgggcgg
     7681 cgaggatgtc gaagccgtgg cggctgaaga tgcggcagag gcgggcgaac aggcgcgggc
     7741 cgttgggcat gaaaaccatg acttgaaagc tgtcgctttt gggcaggatg cggctgcgga
     7801 cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg ccacaggatt tcgcgggact
     7861 ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag ttttttctgc tgtttttcgg
     7921 ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg gcggcggccg aagagggtgt
     7981 gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc atggaagagg ctttccagca
     8041 ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca
     8101 gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaaa gcatcgagta
     8161 cgtcggggtc ttggatgtct tctttttggg cgacggcaga catcagcagg tggttttcga
     8221 ccagccaggc gagcaggtcg ctttcttctc cggtcaggaa gtggtcagcg gcaaattggc
     8281 gcgcgtctgc gatgccttgt atggcatggt cgccgccgcg tcctttggcg atgtcatgga
     8341 aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt
     8401 agggcagctc gtggctgtgc atatccaagg caaggcggcg gacgttgcgg acgacggtga
     8461 ggatgtggtc gtccacggga tagatgtgga acaggtcgtg ttggagcagg ccgatgattt
     8521 tttcccacgc gggcaggtag cggcccaaca cgccgtagag gttgagaaag cgcagggtct
     8581 gggtcagccc gttgccgttg cggaaaaaac cggcgaagcg gcggcggttt tcgggatttt
     8641 ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca ggcgcgcagg gtttgcggtt
     8701 cgagcgcggt aatgtcgttg cgctgctgca tgatttcgac gattttgaaa atgtgttcgg
     8761 gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg gttgttgact tggatgtagt
     8821 cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga aacgcggctt tgcagcatag
     8881 gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca
     8941 gttcttcgct ttggcggcgg aggttcaagc cttcataacc catgctttcg gcgacttgcg
     9001 gctgcaaatc gaacagcagg cggtcttcgg cgcgattggc gtttaaatgc agatggatgc
     9061 ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa
     9121 tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa
     9181 gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc tccaatactg
     9241 cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccagtttt gcctcgacaa
     9301 acgccgccac attgcgctgc gcgttcattt tttccgccag tttatccacc gtttggcggt
     9361 tgccaaacaa aaacctagcc tctaaaaacg ctgtgtcccc cgtaatatca ttgcgcacgc
     9421 tttcacatag ttcatcaacg ctgccgcttt ttacagacgg catcagtttg cagtcccaca
     9481 gggtttgaac aaaccgggca atctgttcct gaatgccgtc tgaaagcggg gcaggggaga
     9541 caaccgccaa atccacatcc gaacagggat acagttcgcc gcgcccgaag ccgcccaccg
     9601 ccatcaggca taacgcgctg ttttgaaaat gttctaccca caatgccgcc agtaaggttt
     9661 cgactgccgc cgtatattcc ctgaaaaata ccgacacgcg gttggctttc aaataatgcg
     9721 cttcggcggc atcgcgctgc cgtttgaagg tttccagtgc tgaagacagg tttgcaggca
     9781 tttttattct ttcgattggc gggaaaaggg aggtggatgg ttcggcggtc aaataccgct
     9841 ttcagacggc atttgtcggg tatcggcgga attggtcatc agcttcaacc gttcctgcgg
     9901 cggcagcagc aactgatacg ccgccacgcc caaagccgcc gccatttttt cgatattcga
     9961 cagggcgatg ttccagcgtt tgcgctcgac tgccgacaca taagtcctgt ccaaaccgca
    10021 ttgccgcgcc aattcttctt gcgaccaacc cttgttcacg cggaaaagcc gcatattgta
    10081 tgccaatacc gcccgcaaat cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt
    10141 gcccatcatt tgcttccaaa taaatggtta aagttaagca tttgctgtta cggattttac
    10201 ttcacataaa agccaaaaaa aaggggtggc ggcagccaca cccaaacaca cacacatcaa
    10261 gaggaaagag gtaacatcag gacagattgg gaaacagcaa acaagcaatc tagatttcat
    10321 atcggtatta tcaaacaaaa accgttatcg agtattgatt caaatcaatt gagatttgat
    10381 tcgcaaaccg gtaaagaaaa acggcatacc cgtttatacg gatatgccgt ctgatgatgc
    10441 aggggactgc tgtatttggc agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag
    10501 gtagttcggc aaaccaattt tgccgattag ttcctgctgg gtttcgagcc agtcgatgtg
    10561 ttcttcgttg gtgtcttttt gtttttccaa caaatcacgg ctgacataat cctgttgcgc
    10621 ttctgccacg gaaattgccg caatcaatgc ctcatgtttt tctttctctt tttccaaatc
    10681 gcaggcgatg atttcttcgg tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt
    10741 cggcagacct tcgaggaaca gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac
    10801 gatggactgt ttgaagaaat gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg
    10861 ggcgtgaagg aaatattggt tgatggttac cagcagcaag cctaagtttt tgtttagctc
    10921 gcggataacc agacggtcgc ctttcatacg gactcctttt attccgaata cggattacag
    10981 ttggctttgg tagtagttgc cttcgccaat taactcgatc aggcgaagct gctgttccag
    11041 ccagtgggcg tggtcttctt cggtatcttt cagttgggca accatcaggt cgcgcgtaac
    11101 atagtcttga gcctcttcgc acagtttgat gccttttttc aacgcatcac gtacttcata
    11161 ttcggtttgc aggtcggctt tgaggcagga aaccacgtcc gtgccgatat tcagttcggc
    11221 gcgcgccatt ttcggcgtgc cgcccagcat caggatgcgg cggatgaagt cttcggcgtg
    11281 tgtggtttct tcttccatct cgtgattgag acgttcaaaa agtttggtgt agccccattc
    11341 ggagtagagg cgggagtgga taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa
    11401 ttcgttcata taatcaacaa cagcttgatt gccttgcata atatctctct ttctgtaact
    11461 tggttttcgg tatgtctgaa cagcatctgt gtcgctattc gggatgcgtg cattgtatgc
    11521 aaaagctgtc ggcatgacaa attttctatt taaaatacaa acaattatca aaagaaatag
    11581 ggcgtattcc ccgatgcctt ccaaatcagt atgctgtttc ttatcggttt ttttgctgct
    11641 aaaactaaga atccatctca tccataaaga acatttacac ttattaacca tagcgaaaag
    11701 caaatactgt acggtttttt atcaaaattt ataatgaatc gttctcatta accgacagat
    11761 tcttttgaga ttatcggatt ttgggaataa attatgccgt ctgaggggct ttcagacggc
    11821 atagacaatt aaagctgatt aaacaacgcg gcagacagca gcgtaggaaa ttatttttta
    11881 cttcggaacg gtattttggg tttaatgatg cccgcaaccg ccgtggcttt cgcgtaaggc
    11941 ttcggcagcc tgttcgtcgg cgtggtagct ggaacgtacc atcgcgccga tggcggcatt
    12001 gctgaagccc agttcgtatg cttctttttc aaagattttg aactgctcgg gcgtaacgta
    12061 gcgcaagaca ggcaagtggc cgtctgaagg ctggagatac tgtccgatgg taatcatctc
    12121 gatattgtgc gcccgcatat cgcgcataat ttcgcgcacg tcttcgtctg tttcgcccaa
    12181 gccgaccatg atgccggatt tggtcgggat gtgcggcatc atttctttat aacgttttaa
    12241 taagtctaaa gaatgttgat aattggcacc gggacgggct tttctgtaca ggctcggatg
    12301 ggtttctagg ttgtggttca tcacgtcggg cggggtttcg gcaaggattt tgagtgcgat
    12361 gtccaagcgt cctcggaagt cggggacgag gatttcgatt ttggtgttcg ggctggtttc
    12421 gcggatggct ttgatgcagt cggcgaaatg ctgtgcgccg ccgtcgcgca ggtcgtcgcg
    12481 gtcgacggag gtgatgacga cgtaacgcag gttcatggct ttgacggatt cggcgaggtt
    12541 tttcggttcg tcggggtcga gcatattggg ccgaccgtgt cccacgtcgc agaacgggca
    12601 gcggcgggtg cagatgtcac ccataatcat gaaggtcgcc gtgcctttgc tgaagcattc
    12661 gccgatgttg gggcaggagg cttcctcgca aacggtgtgc atcttttgtt cgcgcaaaat
    12721 gtctttgatt tcaaagaatt tgcgcgatgg gagtttggcg cgtatccatt cgggcttttt
    12781 cagtttctcc tgaaggggga cgactttgat ggggatgcgg gcggttttgt ccgcgcctct
    12841 gagtttgatg ccgcgtttgg ggtcgtcggt tttgatttca ctcattgttg tctgctttcg
    12901 gtgtgaattg tgttgcaagg tgtgcggtaa gtttggcggc gacttcgtcc ggcgtggggc
    12961 agggttggac aaaatccgcg atttgcgtca tttccgtacc ggcgtagccg caggggttga
    13021 tgtgggtaaa cgggcttaaa tccatattga cgttgagcgc aagcccgtga tagacggagc
    13081 cgtttttgat acgcagcccc agtgaggcga ttttgcgttc tccgacataa acgccggggc
    13141 gtttggggtc tgccgccgct tcgatgccgt attctgccaa tgtggcgatg atgctgtttt
    13201 caagcgcgga aacgatgttt ctaacactgg ttttgcgccg tttgaaatca atcatcgtat
    13261 aaacgaccaa ttgcccgggc ccgtgatagg taatctgccc gccccggtcg atttggacga
    13321 cgggaatgtc gtcgcgaatc agcaggtgtt cgggttttcc cgccagtcct tgtgtgaaca
    13381 cgggcgggtg ttcgacgacc cacagttcgt cttcggtgtc ggcattccgt ccggcattaa
    13441 aggttttcat cgcttcaaaa gtcggcagat attcgaccaa acctttgtgt atgattttca
    13501 tctcaaagta ccactttgac cagttcgtgc gaagtcagcg cacggtagat gttgtccaat
    13561 tgttcttggt tttcaacctt tacctgtacg gtggcgccag tatagttgcc tttgctgctc
    13621 ggacgcgtgg tgatgtggtg cgcctgcgtg tcgggggcgt ggaggcggac ggtgtctaaa
    13681 accgcctgct cgaactcggg atgcaccgcg cccattactt tcaatgggaa ggtgcaggga
    13741 aattcgatga gggatgtttt gtttttttgt tcggtcatga tgtgctgcct tatcgtgtac
    13801 ggtatgccgt ctgaaggcgg gtttgccttt cagacggcat cggatgtgcg ttattttagc
    13861 ctaaaccgcg ataacaggct atcgggaagg cggaggcttt tttgacggcg cggcggttct
    13921 gctatactgg cgcacaatat tatttttaga agggtggttt ttatgtatcg gaggaaaggg
    13981 cggggcatca agccgtggat gggtgccggt gcggcgtttg ccgccttggt ctggctggtt
    14041 ttcgcgctcg gcgatacttt gactccgttt gcggttgcgg cggtgctggc gtatgtattg
    14101 gaccctttgg tcgaatggtt gcagaaaaag ggtttgaacc gtgcatccgc ttcgatgtct
    14161 gtgatggtgt tttccttgat tttgttgttg gcattattgt tgattatcgt ccctatgctg
    14221 gtcgggcagt tcaacaattt ggcatcgcgc ctgccccaat taatcggttt tatgcagaac
    14281 acgctgctgc cgtggttgaa aaatacaatc ggcggatatg tggaaatcga tcaggcatct
    14341 attattgcgt ggcttcaggc gcatacggga gagttgagca acgcgcttaa ggcgtggttt
    14401 cccgttttga tgaggcaggg cggcaatatt gtcagcagta tcggcaacct actgctgctg
    14461 cccttgctgc tttattattt cctgctggat tggcagcggt ggtcgtgcgg catcgccaaa
    14521 ctggttccga ggcgttttgc cggtgcttat acgcgcatta cgggtaattt gaacgaggta
    14581 ttgggcgaat ttttgcgcgg gcagcttctg gtgatgctga ttatgggttt ggtttacggc
    14641 ttggggttgg tgctggtcgg gctggattcg gggtttgcaa tcggtatggt tgccggtatt
    14701 ttggtttttg ttccctattt gggcgcgttt acaggactgc tgctggcaac cgtcgccgcc
    14761 ttgctccagt tcggttcgtg gaacggcatc ctatcggttt gggcggtttt tgccgtagga
    14821 cagtttctcg aaagtttttt cattacgccg aaaatcgtag gagaccgtat cggcctgtcg
    14881 ccgttttggg ttatcttttc gctgatggcg ttcggacagc tgatgggttt cgtcggaatg
    14941 ttggcaggat tgcctttggc cgccgtaacc ttggtcttgc ttcgcgaggg cgtgcagaaa
    15001 tattttaccg gcagttttta ccggggcagg taggcggttc cgaaacatat ttgaagcgga
    15061 atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac gggacttcag
    15121 acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt ttcataccgg
    15181 cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta ttgctttctt
    15241 tggcaagatt gttgaacgcg gcgacgtatt gttccgcaat cttcagattg accgcatccg
    15301 caccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca ttggcttcgg
    15361 caacaaggcg caaggattcc gcttcacctt tggcgcggtt gatgcgggcg attttctcgg
    15421 catttgacgc attgaccgca gcctgagcct cgccttcgga ttgttggatt tcggcttcgc
    15481 gctgaccact ggcaaggttg atttgttcga ttttacgacc ttcggattcg gcgatacggg
    15541 cgcgtttttc gcgttcagca gtaatttgcg cctgcattga gcgaaggatt tcttgcggcg
    15601 gaaccaagtc tttaatctca taacgcaaaa ccttcacacc ccaagctccg gcggcttcat
    15661 cgagggcgga gacgacggtg ctgttgattt cgtcgcgttc ttcaaacgtt ttgtccaatt
    15721 ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc gccataatgt
    15781 agttgctcga accgtatgag gcgagtttgg ggtcggttac ttggaaatag atgatgccgt
    15841 caaccgtcag ctgggtgttg tcgcgcgtga tgcagacctg gctgggtacg tctaaaggga
    15901 tttctttcag cgaatgacgg taggcgacgc ggtcgataaa gggaatcaaa atattcaaac
    15961 cggccgtcaa ggcgcgatgg aaacgtccaa gcctttcgac aacgtggact tcctgctgtg
    16021 ggatgacgac aaaggatttg aagccgaaaa cggcaacggc taccaacaag ataatgaaaa
    16081 attccataat tcctccgagt gttaagggtt tgcgatgata agaaggttgc cttccttgcg
    16141 gacgattagg gcgcgagttc ctggttcaag ctcttcttgc cccgtatttt gagcctgcca
    16201 gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gtgtgtcgga ggatttcgac
    16261 atattgtccg gcatccaaat cctgatatga atccgtttca acttttccca cggcggtttt
    16321 ggcgtgtacg aaccaaatac ccagcgcgga aagcagagca gcggtcaaga cggcggcagg
    16381 cgtgctgccg gtcagtccgt agataatgcc cgaacccgcc aaagccgcgc tgacaaccag
    16441 aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcag caacaaacca
    16501 tacagtcata catttcccca caaagcgcgt cgtttgacaa aatcacgcga tatcagcagt
    16561 atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca ctgggcaacg
    16621 aaaaacggca accgtatttt caggaaattt taaatgcagt caggcaggaa cgtttgtcgg
    16681 ggcaaatcat ctatccgccg gcggaggatg tgttcaacgc attccgcctg acagcgtttg
    16741 accgagtcaa agtcgtcatt ctcggacaag acccgtatca cggggcaggg caggcgcacg
    16801 gtttggcatt ttccgtcagg cagggcgtcc gcataccccc gtctttactc aatatctaca
    16861 aagaattgga aaccgacatc gaaggctttt ccattcccgc gcacggctgc ctgacagcgt
    16921 gggcggagca gggcgtatta cttctgaaca cggttttgac ggtgcgtgca ggacaggcgc
    16981 attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg cagcttgcga
    17041 cacaccgcaa gcatcttgtc tttatgctgt ggggtgggta tgcacaacaa aaaaggaaac
    17101 tgatagacag tcaaaaccat ttgatattga ccgcaccgca tccgtctcct ctgtcggcat
    17161 atcgcggttt tttcggttgc cgccattttt cacaggcaaa cagctatttg agccggcacg
    17221 gtatcgatcc gataaactgg aagctgtgaa tgccgatata gccgttgccg ccggcgtgtt
    17281 aaaatcgcgt ttgatttgta atttccattt attaggcaaa accttatggc aatcaaaaaa
    17341 aaatcagctt tatgcatcac tttgggcggg ctgcgacgag ttgcgcggcg gtatggatgc
    17401 gagcctttat aaagaactat gtgcttacgc tattgttttt aaaatatgtt tctgataagc
    17461 ataagtacgg cggcggcatg attgagctgc acgccggtac gacttttgac gacatcgtca
    17521 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
    17581 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
    17641 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
    17701 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
    17761 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
    17821 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
    17881 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
    17941 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
    18001 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
    18061 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
    18121 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
    18181 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
    18241 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
    18301 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
    18361 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagt ggcggcagcg
    18421 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
    18481 agcaagacat tcacaaaatc atcgacactt ccacaaacct cgttacagcc gtatggtgca
    18541 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
    18601 agaagttgaa gacctgcaaa atctcgccgc ctatctttat ggcggcatac ctgcgcacga
    18661 tatggacgca ttggaagcct attggcaagt tttaggccgt atgaaaaacg agttgtttgc
    18721 cgaacacgat ggccgcttta ccactataca acggaatcga ttgcaaatct ttcccactct
    18781 caacagctta aaatcctgcg ggattggtgt ggaatttagg gctaatctag tacagcccca
    18841 aatttaatcc actataaaat cgaaagcagc caaatcaaag cccatatatt ggcgcacccc
    18901 gattacgccg ccttcaaagc cggacacctg gcaaagtttg ccgcgtggca cactcaaaac
    18961 gaccttgccg ccatccaacc gggcaggctt atccggaaat ggagcgaaag cctgctggac
    19021 gcgttcaaac ccggcagcct gattgaagaa tacgatttct accaaatcct gacggactac
    19081 tgggcggaaa ccctgcaaga cgatgtttat ctcatcgccc aagatggctg gaaggcggtt
    19141 aaaaacctgg ccgaaatcac caaagaaagc gatgaagacg cgaacctgac cgtcgtcttt
    19201 gaggaaaccg aaaccggcaa aaaaggcaaa gccaaaacca agcgcatcag caaaaaatac
    19261 cgcagcgaag tcatcgcccc cgagctggtt gcccgccgct acttttcaga cggcatcgcc
    19321 aagctggaag aaaaacaaag cgagctggaa cgcctaagcc aagaattgga aaaccacata
    19381 gaagaacacg gcggcgaaga gggtgcgctg aacgacgtat tggatgcaaa aggcaaactt
    19441 tccgccaaac ttctgaaaac cgcattggaa gaaagcggca tagaagaagg cgaacgggct
    19501 gttttacaaa ccacccaaac actgatgacg caggaaaaag ccgcgaaaga cgcagtcaaa
    19561 acccaaatcg aagccctgaa ccttgccgta ttcaaacaat tcggccgact ttccgaagcc
    19621 gaaatcaagc agcttgccgt tcaagacaaa tggcttgccg atttacaaag ccgaatcgaa
    19681 aatcgcttgg aaaacagtat tcagcagctt atcagccgct tgaacacgct ggaagaccgc
    19741 taccgcagcc cgatggccga gcttgcccga gaagtggaaa agtggcaaag caaagtcaat
    19801 gcccaccttg aaaatatggg ttttggaggc tgaaatggcg gcacagacag gctataaggc
    19861 gagcgggttt tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat
    19921 ttaggggatc gcggttcggg tgtccgcacc gcttaatacg tcgtcgtcca cgaactgacc
    19981 catttgctcg aacgccatcg caacgcccgt tttatggcgc atatggacaa ctttctccca
    20041 aactggcaaa gcatcaaaca acagcttaat gccttggagt tatttgcaca aatatataat
    20101 ttaacataat atatattatg cgaactatcg gaaacagttg tacgtgtccc tgtggtcttt
    20161 ccaagtagga aaattaaagt atggacaatg cggctgaatg tatagcccgg agcatccgcg
    20221 tatccgaagg tactgaacga tatgccctgc atccctttcg acgcttccat ccagccggta
    20281 ttgggaaata cgttgacctt caatacgata tacaggcagc cgacacacag gatatgccgc
    20341 tgctttttca tcatttcttc tgtcaaatcc ttggaacagt tgatttgaaa acacggcttt
    20401 acatacgtcc caattctctt tgcagtgctt ggatattttg ctgcctgtcc aatacattgc
    20461 tttgcaatgc gttgatttct tgatggttga tgttgccgcc ttttgccaga cgtgcttgtg
    20521 ataacatttt ttgggcttca accaatgctt tgcgttcgtt gctcaattct gtttcgagaa
    20581 tggagcgtct gctgttgttt gagggtgctt gttgcggcgg cggcgtattg gattttgccg
    20641 gcttggatac tgttttgacc ggggctttat atttgacagc ctgtccgccg tttgacggtg
    20701 atggtgtcgg ttcggatgtt tggggcggga tatagcgttc gctgctgtag ttgccgattg
    20761 ggggcaaatc ggttgagagg cagcttttgg acggcttggt ggtgtaaacg gtttctccgt
    20821 tgattgtgca ggtgtagatt ttggccgcat tcgcacccaa tgggcttgaa atcagggaaa
    20881 agttgattag gattaagggg agttttgatt tcataatgtg cttgatgctc ggataattat
    20941 ttgatttttt ggtatttttt gtaatgcttt cttggcgttt tacgggcttt tcttttcggt
    21001 tggttgcttt tgattttctt tcggggcttt ggcagcctgc ttgttctttt tcccggcagg
    21061 ttttttgttt ttctttttac tttctatgtg tattttggct tcttaactga gttttttaat
    21121 tttcaggcgg tatccgcctc cctgatggct gctgatttta ggtaaatccg catcggcgca
    21181 cgatcctgct ggggctgatg tatatacgct tttgccattt gagttgcaat ggtatacgga
    21241 ggcttgcgcc gccgcgccag aaagggacag gagacccaag gcggcaaaaa gtctgatgtt
    21301 tataccggta aaaggcggtg ataacgcccg aattataccg ttattggcag gcaaagataa
    21361 gcaccctgcc cgcgcttctt tatcgctcgg caaactgttt ctcgatttca gtttcaatcc
    21421 gttccaattc ttcttgacaa gccagccaaa cctcttcgat ttgggcaagt tgtgttttga
    21481 cttttgccag ctgggataag gtattttgca atttttcttt gtttgcttca aggtaagcgt
    21541 cttcttgtgc taaaaatgct tcacatgccg tctgaatttc ggaaagctgc gccatttctt
    21601 tttcggcacg gtctattttc tgctgtatcg gcttgccgcg tcgggctttt tcctgacgga
    21661 tttgcgcttc gatgcgcttg gtgtctttgc ggctttggct ttgtgcggat gctgctggtg
    21721 cggcggcgtt ttcctgtgcc aaacgccatt ggcggtaatc gttcaaatcg ccgtcgaagt
    21781 tcttcagacg gcctttgtcg atcaggagga agctgtcggt agtcgcttca agcaggctgc
    21841 ggtcgtgcga tacggcgatt aatgcgcctt ggaaactttg caacgcgagc gttaaggcgt
    21901 ggcgcatatc caagtccaaa tggttggtcg gttcgtcaag cagcaacagg ttcggctttt
    21961 gccagataat catggcaagg gcaaggcggg ctttttctcc gccggaaaaa ggttcggttt
    22021 tctgtaacgc catatcgcca acaaaattga agccgccgag gaaatttcgg atttcttgtt
    22081 cgcgtacttc gggagaaagc tgctgaatat gccaaacagg gttttggtcg gcgcggatgg
    22141 tatcgagttg gtgttgggca aaatagccga tattgagttt ttcggaacgg acgatgctgc
    22201 cggagagcaa atcgattttg cccgccaagg ctttgataaa tgtagattta ccgctgccgt
    22261 tgacacccaa taaaccatag cgcgcgccgc tttccagcga cagggtaatg tcgtgcaaaa
    22321 cagttttgcc ttcgtaaccc aaatctgcgt gttccagctt cagcaaagga ttgggcagat
    22381 gctcgggatt gtaaaactca aaggaaaact cgctgtccag atgcgcgggc gcaacgcgtt
    22441 ctagctttgc caaagccttc atgcggcttt gcgcttgaac ggctttggtg gctttggctt
    22501 tgaagcggtc gataaaggat tgcaaatgtt tgatttgcgc ctgctgtttg acataggcag
    22561 cttgttgttg cgcgagacgc tgcgcgcgtt cgttttggta aaaatcgtaa ttgccgccgt
    22621 attgcgtgag tttttgctgc gataattcaa tggtttgggt agttgccgcg ttgagaaaat
    22681 cgcggtcgtg ggaaatgatg atttgcgtgc agggtaaaga agcaagatgg ttttccagcc
    22741 acaagacggt ttccaaatcc aagtggttgg tcggttcgtc caaaagcagc aaatcggcgc
    22801 ggcaaatcag ggcttgggcg agattgaggc gcatacgcca gccgccggaa aaggatttga
    22861 cggggcggct gtgttcttct tgtgaaaaac ccagcccgtt caacaatttt gccgcgcggg
    22921 ccggcgcggt ataagcgtcg atttcttcca atttggcatg atattccgcc tgcttcatgc
    22981 cgtcattttg cgcttccgcc tgcctcaatg ccgtctgaaa agcctgcaac tcggcatcgc
    23041 cctgcaaaac gtaatccaaa gcggaaatat ctaaatcggg cgtttcttgg gaaacggaag
    23101 cgagccgcca gtttttcgga atcgagacat cgccgccgtc ctgagtgatt ttacccttga
    23161 ttaaggcaaa caggctcgat ttgcccgttc cgtttttgcc gatcaaaccg acgcgctgac
    23221 cgggattgac ggtagcgttg gctttgtcga gcaggacttt caaaccgcgt tgcagggtga
    23281 ggtttttgat ttcaatcata acggaaacat cgtcgggcgg aaaaagcccg tattttacct
    23341 gaaagtcagt gccgatgccg tctgaaatgg gaaatttacg gctgaagcca agcccaagcc
    23401 ctgcgccctt ccgagtgcag gaaaaccaat gtcctgaatg ccgaatcggt attcatgcat
    23461 tccacgctga ttccgattcg ggaaaaatcc gccatgattt tgggatggat aaactcctga
    23521 gccgcgcccg tcccgataat caatatttcc ggatagtcaa caggtttgat gtcggacaac
    23581 aggttttccg gagtcagatc ggacaaggtt cggcattgcg acaggcagac cgaatcctta
    23641 tgtacaagca cgggtttatg gaaactttgc cccgtcagcc ggattccgcc cgcaccgcat
    23701 tcatattccg caaactgtcc gtctatcgga ttttcttcaa acaacatttt tttaccccgt
    23761 cgccgcatca tctacaccga aaggggtgca aaatcaggcg aattcatgta ggattggcag
    23821 atttcatctg acccgcctgc cgatttcaga cggcatttga ttcaaagtgc ggcacaatta
    23881 tatcgacagc ggatattttc gtctttcaat atttacattt cagtcggctt acaaggagac
    23941 acaatgaagc cagtaaacat cggtctttta ggtttgggta cggtcggcgg cggtacggct
    24001 gccgtgttgc gggacaacgc ggaggaaatt tcccgtcgct tggggcgcga aatccgtatt
    24061 tctgccgtgt gcgatttgag tgaagaaaaa gcccgacaaa cctgcccgtc cgcagccttt
    24121 gtcaaagatc cgttcgaact ggtcgcacgt gaagacgtcg atgtcgtcgt cgaattgttc
    24181 ggcggtaccg gcattgccaa agatgcggtg ttgaaagcca ttgaaaacgg caaacacatc
    24241 gttaccgcca acaaaaaact gctcgccgaa tacggcaacg aaatcttccc gctggcggaa
    24301 aaacaaaacg tcatcgtcca atttgaagcg gcagtagcgg gcggtatccc aatcatcaaa
    24361 gccctgcgcg aaggtttggc ggcaaacagg attaaatcca tcgccggcat tattaacggc
    24421 accagcaact tcatcctctc cgaaatgcgc gaaaaaggca gcgcgtttgc cgatgtactg
    24481 aaagaagcgc aggcattggg ttatgccgaa gccgatccga ccttcgacat cgaaggcaac
    24541 gatgcgggcc ataaaatcac catcatgagc gcactggcat tcggcacgcc gatgaacttt
    24601 tccgcctgct acctcgaagg catcagcaaa ctcgacagcc gcgacatcaa atacgccgaa
    24661 gaacttggct atcgcatcaa actgttgggc attacccgca aaaccggcaa aggcatcgag
    24721 ctgcgcgtcc accctaccct gattcccgaa agccgcctct tggcaaacgt caacggcgtg
    24781 atgaacgccg tgcgcgtcaa cgccgatatg gttggcgaaa ccttatatta cggcgcgggc
    24841 gcgggcgcat tgccgaccgc ttccgccgtg gttgccgata tcatcgacat cgcccgcctg
    24901 gttgaagccg ataccgccca ccgcgtaccg catctggcgt tccaacccgc gcaagtccaa
    24961 gcgcaaacca tcctgcctat ggacgaaatt accagcagct actacctgcg cgtccaagcc
    25021 aaagacgaac cgggcacgct ggggcaaatc gccgcgctgt tggcacaaga aaacgtgtcc
    25081 atcgaagcac tgattcaaaa aggcgtgatt gatcagacca ctgccgaaat cgtgattctg
    25141 acccacagca cggtcgaaaa acacatcaag tcggcaatcg cagccatcga agcactggat
    25201 tgcgtggaaa aaccgattac catgatccgc atggaaagcc tgcatgactg agccgaaaca
    25261 cgaaatgctg acgaaagagc aggttgccgc gcgcaaaaaa gcaaaagcca aaatccgcac
    25321 catccgcatt tgggcgtggg tcattttggc gttgctcgct ttaaccgccc tgctctccca
    25381 atgcgcgatg tccaaaccgc aggcaaaaca gaaaattgtc gagtcttgcg tgaagaatat
    25441 tccgtttgcc gaaaaatggc aaaacgattt gcgggcccgc ggtttagatt caaacaatac
    25501 ccgcctcgcc gtcgactact gcaaatgtat gtgggagcag cctttggaca gattgagcga
    25561 gaaacagatt agatccttcg gcaaactcgg cgcacaagaa cagcttgacc tgctcggcgg
    25621 cgcaaatgcc tttgaagcac gtgacaagca gtgtgttgcc gatttgaaat cagaataatg
    25681 tggaccgata aaaaagccga ttctttaaag aatcggcttt tttcataaaa aacggcttac
    25741 agtgcgtctt tcaaagcttt gccggcgcgg aatttaggcg ttttggcggc ggcaatggtc
    25801 agaggctcgc cggttttggg gttgcggcct tggcgttccg cacgttcgcc cacgtagaaa
    25861 gtaccgaaac cgaccaaagt aacggtgtcg ccttgtttca gggcggtggt tactgcattg
    25921 gtagtggcat ccaaagcttt ttgtgcggcg gctttggaaa tgtcggcttc ttgagcaatc
    25981 gcttcgatca attcagactt gttcacaatc agtcccttcc tgtcttaaaa aatgatgaaa
    26041 tgcccgaata ctcggggttt gtactgcttg agcaactttc gctttatagc aattctgaaa
    26101 ttgccgtgtc aagcaaaaaa tacggaatca ccctatttga caggctttca ggacgaaacc
    26161 gcatttttac aacacatttc ctgcgtttca atgtttggtt gccctgctgc ggggttttgg
    26221 ttttgaagcg gattccgccg ccgcttccgc accagaaggt tctgcccaag gctcaggctg
    26281 gctttccaaa cccagagcca atacctcgtc tatccatttg accggatgga tggtcaggcc
    26341 ggttttcacg ttttcaggga tttcttccaa gtctttgagg ttgtcttttg gaattaaaac
    26401 gtgtttgatg ccgccgcgaa gcgcagccaa cagtttttcc ttcagaccgc caatcggcag
    26461 gacttcgccg cgcagggtaa tttcgcccgt catggcaaca tcggcgcgca ccgggatttt
    26521 ggtgaatgca gacaccatcg ccaaagtcat ggcaatgccc gcactcggac cgtctttcgg
    26581 cgtcgcacct tcgggaacat ggacatggat gtcttttttc tcgtaaaaat caggagccaa
    26641 gcccactgat tccgcgcggg agcggacaac cgaccacgct gcagacacgg attccttcat
    26701 cacatcgccc aactggccgg tgcactgaat cacgccctta cccggcaatg ctgcggcttc
    26761 gacggtcagc agttcgccgc cgacttctgt ccacgccaga ccggttactt gtccgatacg
    26821 gttttcgctt tcggcaacgc cgtaatcgaa gcggcgcaca cccaaatagt cgtgcagatt
    26881 tttctcattt actttaaccg ctttaggttt ggctttgctg gttttcttgg tttcagacaa
    26941 cctcttctta tcttcgtcca aggtaatctg catcaccacc ttgcggcaga ttttggcaat
    27001 ctcgcggtcg agcgaacgca cgcccgcctc gcgggtgtag taacggataa tatcgcgcac
    27061 cgcgctttct tcgattgcca attccccttc tttcacgccg ttgcgcttca tttgcttcgg
    27121 cacgaggtac tgcatcgcga tattgatttt ttcgtcttcg gtatagccgg acagacggat
    27181 gatttccata cggtcgagca gcggggtcgg gatattcagg ctgtttgaag tcgcaataaa
    27241 catcacatcg ctcaaatcgt aatccacttc ggcataatga tcggcgaact tgttgttttg
    27301 ctcgggatca agcacttcga gcaacgcgct ggcgggatcg ccccggaagt cgctacccaa
    27361 tttgtcgatt tcgtcgagca ggaacaatgg gttttttacg ccggcttttg ccatattctg
    27421 cagaatctta ccaggcatgg agccgatata ggtgcggcgg tgtccccgga tttcgctttc
    27481 gtcgcgcacg ccgcccaaag ccatgcggac atatttccgc cccgttgctt tggcgatgga
    27541 ttcgcccaaa gaggttttgc ccacgcctgg agggccgacc aggcacagaa tcgggccttt
    27601 gagtttgtcc atacgttttt ggacggcgag gtattccaaa atccgttctt tgactttttc
    27661 caggccgtag tggtcggcat ccagcaccag tccggctttg gcgatgtctt tgctgacgcg
    27721 ggattttttc ttccacggca gttcgagcag ggtgtcgatg tagttgcgta cgacggtgga
    27781 ttcggcagac atcggcggca tcattttgag ttttttcagt tcggacaggc atttttcttc
    27841 cgcttcttta ctcatacccg cctttttgat gcctgcttcc aaggcatcca gttcgccgtt
    27901 ttcgtcttct tcgcccaatt ctttgtgtat cgctttaatc tgttcgttca gataatattc
    27961 gcgctgggat ttttccattt ggcgtttgac gcgtccgcgt atgcgttttt cggcctgcat
    28021 aatgtcgagt tcggattcca gctgtgccag caggaattcc atccgtttgc cgatttcggg
    28081 aatttccaaa atctgttggc gttgcgccag tttcaactgc aaatgcgctg cgaccgtatc
    28141 ggttagccgg ctgttttcgg caatgccgtt aatgctgccg ataatttcgg cggggatttt
    28201 tttattgagt ttggcgtatt gttcaaactg cgccaacagg gtgcggcgca cggcttcgag
    28261 gtcggtattg ccgcccgtgt cttcttccac gaccgcctct atatgggaaa cgaacagccc
    28321 gcccgtgtct tcaatggtca gaacacgtcc gcgatacagc ccttcgacca atacttttac
    28381 cgtgccgtcg ggtagtttca acacttgcag gacttgtgcg accgtaccgg tctgatacag
    28441 gtcggcggca accggttctt ctaccgccgc atcggtttgc gccaacagga aaaccggctc
    28501 ctcgcgggta atggcgtttt ccagtgcggc gatggatttc ggcctgccga caaacagcgg
    28561 cagaaccata tgcgggtaaa cgacgacatc ccgcaaagga agggttgcca aggcggcata
    28621 ttcctcaaaa tgcttttctt tttgtgtcat aggtactctc ttgtgtctga cagattgccg
    28681 attttcgcgt acattggggt tgaaggtatt atttcaagca tatgtggttt atttatggag
    28741 tttgatgcga tgccgtctga aacattccgg cttcagacgg catgggcttg gaaagacaag
    28801 gcgggaacaa aaaactgttc tgtgttgccg ctccttgctg taccatccgt atggtttgcg
    28861 gttctgccgc cctattttca aaacatgacg caggtattcc atgtcttctt ttatttttcc
    28921 cgataacggg ttcgaatggc gcaacgaaag tctgcaaaag ccgcccaaag gttggcatta
    28981 tgtccgcgaa agtggtgcaa acggcatttt gaaggctgcc tatcaaaata ttgcaactgt
    29041 ctggcagggc gatttccaca atgccaaaca ggtgctttcc gcaatgaaga agaaggtccg
    29101 caaacctgcc gccgtccgtt ccgatgcgga tatcgccgcc cttttccacg cccaccgtat
    29161 gaagcaggcg cagcagagcc gtattctgaa tatgcttgcc gttgaaatcc gccccggttt
    29221 tgtgttggac aacaaacgcg cgcccgatat acgcgccgcc ctgctcgacg tgtacggaga
    29281 ggcggacggc aaaccgtttt tcctgccgct caatctgctg ctggggttta tgggtgcgca
    29341 cgagtggcat aagaaagggg ttgccgttcc gcagctgggc ggcagcatac acgttccttt
    29401 cggcgtattc tcgccgttgc gcggcgaata cctcgacctg ctcgcccatg cgccgtcaac
    29461 gggttttcag acggcattcg atatcgggac gggctcgggc gtgcttgccg ccattttggc
    29521 gaaacagggc attccttccg tcatcggcac ggataccaat ccgaaagccg tcgcctgcgc
    29581 ccgtgccaat attgctcgtt tgggctttga aaaacaggtt gagatacggg aaaccgatct
    29641 gtttcccgaa gggtttgccg atctgattgt ctgcaatccg ccctggcttc ccgccaagcc
    29701 gacttccgcc gtcgaatccg cgttatacga ccccgaatct gcgatgctgg ctgcgttttt
    29761 gcgggatgcg ccgaaacatc tgaatcccga cggagaaatc cgcctgatca tttccgatct
    29821 tgccgaacat ctgcacctgc gtccgtccga ttttctggat aaggcatttg ctcaggcggg
    29881 tttgcgcgtt gcggatgttt tgaaaaccaa accggttcac aaaaaagctg ccgatccgaa
    29941 caatccgctg gcttttgccc gaaacaggga aactactttc ctgtaccgtt tgaaaaaggc
    30001 ataaggggcg gcggtgcgct tccggcggca ggaatctgaa cgcccgcgcc gccggcacgg
    30061 catcattcgg gcggattatc cttgtgaaaa tacccgctca agcatactgc ccaatgccgt
    30121 ctgacgcgtt ttgacagtgg cggcagcttg ccggaaggtt tcttctccgc cgaagaatac
    30181 gaacttctcc ctcgcgcggg taatggcggt atataacagc tccttactca atccggacaa
    30241 tgcatcgtcc ccttcgtccg aaggtgcggc ggaaggcggc agcagccaga cttcccgata
    30301 ttccgaacct tggcttttgt ggacggtcat ggcgaatgcg ggttcaaatt cgggcaggca
    30361 gcttaccgct acctttttaa atccgtccgc atcggcaaaa taggcggcaa ggctgccctg
    30421 ccgtccgaca tcttccataa tcagtccgat gtcgccgttg aacagttcaa gcgcgtagtc
    30481 gttctgcctg atcataatcg gctctccggc aaaatatgcc aaatgttccg gtatgttcat
    30541 tttgcggcgt acatggcggc aataggcttc gttgaagtct tccgcatcct gccgccaagc
    30601 tgccagaacc acgatatccg aaatgcccgc gtatgcggct tcgatattgc cgtcttttac
    30661 cgcctgccaa taggctttgt gtgcccggta caacctttcg actcgagcgt tcggactgca
    30721 ttccgaatgt tccagttcgt ccggaaaccg gtcaaacaat gcccacgccc cttcatcgcc
    30781 cgatacggcg gcacgggcaa ggcagccgat gccgctgttg tcgccgaagc ggtggctgaa
    30841 cgacagatgg gcggtgtttt gcgccaacac gggtggattt gcgctgacgc tgaaaccgtg
    30901 ttccggaagg aagccggcca gcctttggtg cgtttctccg tccaaaacgg ttttttgtga
    30961 caaaacggac agcaccgccc ctattccgac ggacgggagc tggttttcat cccccagcag
    31021 aatcacgcgc gcgccggttt tgaccgcttt taaaagttgc agcatcaatg ccgtatccag
    31081 catagaggct tcatcgataa ccaatacgtc aaacggcagc gggtaaacag ggttgaacgc
    31141 cgcctgcatt ttgggcgggc gcagcttcag cagtcggtgg acggtttgcc cttccagttt
    31201 gagcaaatgg cggcggacgg cctccagcgc gtcaaaaccg ttgattgcac ggtgcagcgc
    31261 gcgcgccata tgtgccgccg ctttgcccgt cggtgcggca agcgcgatgt tgggaagatt
    31321 ttcgtcttta ccgcaaatca gcgccagcag tttggcaacc gttgtcgttt tgcccgttcc
    31381 cggcccgccg gtaatcacca taaaagactg caacagtgcc aaggcggcgg catcgcgctg
    31441 cccttcgctg cccgtgcctt gaaaccattt tgcgaggttt tgcctcgcgc ctgccgcgtc
    31501 gggggcggac gtgtcggctg ccgccaagcg ttttatctcg gcagccaaat cgtattccaa
    31561 ctgccacatc ctgcccaaaa acagccttct gccttccaaa atcaaaggcg cggcggatgt
    31621 tccgacaacg ggtgcgagtg ccgacagcgc gtcagcctcg ccaccgctca aacggataaa
    31681 cgaatgaccg ttttgcaatg cctgaaacag gcgttcggtg cagtttgcaa gcacttcgtc
    31741 gccggaaccc gcatagtgtt ccaaaaaacg gattgccgcc cttgccgccg cttgggcaaa
    31801 ttcatctgtc tgccgttcca ttttattcct tatccaaatg ccgtctgaag gcgtggggct
    31861 tcagacggcg cggtgttgtt tcggctgttt aggcgtttgc gccctgtttg gggtgcaggc
    31921 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
    31981 cgaccaccag ccccgttccc aattctgttt tcagttccag catcatatcc aaaacattcc
    32041 tggcgttcgc acgatcgaga ttgccggtcg gttcgtcggc aagcaggcat ttgggttggg
    32101 taaccaaggc gcgtgcaatg gcggcacgct gccgctcacc gcctgaaagt tcgcccgcgc
    32161 ggtgcgtcga acggtgtttc agtccgacct tttccagcat cgccattgcc gcctccgcag
    32221 cctcttcacg gctttttttg ccgatcagaa gcggcatcat cacattttcc agtgccgaaa
    32281 attcaggcag aagatggtgg aactggtaca cgaaaccgag atggcggtta cgtaaatcgc
    32341 ccaaacgccg ctggtttaag gtacgcaaat cctcgcccat cagcagcacc ctgccttcag
    32401 acggcatatc cagcccgcct agaatatgca gcagcgtcga ttttccgctg cccgaagaac
    32461 cgatgatgcc ggtgctttcc cccgcgcgga tttccaaatc caagccgtgc agcaccagaa
    32521 cgtccaaacc gccgtcccga tagcgtttgc ccacgccttc gcatttcaaa atcaactcac
    32581 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccggctcgg gtagagcgtc
    32641 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
    32701 tcgctgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
    32761 ttttcaaaaa acgccacgac cctgccgacg ttccaaccca aaagcacgcc gcagaccaca
    32821 cccgccaacg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
    32881 ccgccagggg aaagacccaa agtccgtaaa atcgcaatgt ccgcctgctt ttccgtaacc
    32941 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggatg
    33001 atgaacatca tccgtttttc caactcgacc gcctcaaaat agctgcggtt gctgaacgtc
    33061 caatcgcgta cccaaaccgt atccctttgc gcctccggaa tcagctttgc cgtcaaggcg
    33121 ggggcgtttt gcgggtcggc gagtttcagc cgcagccccg caacttcctt atccaagcgg
    33181 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtcaac ctcgtaaaca
    33241 cccgttttaa ccagaccgac cacggtaaac tgtttcaacc tcggtacgac tccggcgggc
    33301 gtaacattgc cctccggcgt gatgacggta actttattgc cgacttccgc ccccaaagcc
    33361 tccgccaagc cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaatttg
    33421 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
    33481 cgtatctgca cgcccctgat ttcgcccgca ttggccagca atgcctgatt ggaaacatag
    33541 ggcgcggcag ccaaaatacc tttgcggttt tcggtaaacc gaagcaggtt gcgccaatcc
    33601 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
    33661 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
    33721 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
    33781 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
    33841 tgtctgcaaa cgtgccgccc ccggacggcg gtttgggggc gacggcaaaa gtttattgta
    33901 ccgtaaaacc caggcagcgt ccgaacgccc gagtgcgggg gacggggcgg cagagcgggc
    33961 ggaaaacttg cacaacgcgt caaactgccc tatcctttcc ttcagaaaaa ccgtttcttg
    34021 aggaaaacaa tgaatatccg aactgctttt gctttgtgcg ccatcgcctt atccgccgct
    34081 gcggctgcct acgccaaaga aatcaaaatc gatgccaaca acacgcctta ttccgaagcc
    34141 gacgcgcaaa agctggcggc aacggcagtc ggtatgggtg ttaaggaacc tatcagcctg
    34201 aacggcggca gcggcagcat taccgtgtcc ggcagcagcg cgacgcagtg cgtgttcaaa
    34261 gtcggcaacg gaggcgcgtt gcagattcaa gggctaaact gcaagtaaac cgcccggaaa
    34321 aatgccgtct gaaggcttca gacggcattt tgcattggcg gcgttatgcc ccgccttctt
    34381 taatcaggcg gcgttcgtac accgcctgcg ccagcgttcc cgcatcgaca tattccaatt
    34441 cgccgcccaa gggaatgccc tgcgacagcc tgctgacttt gtaaggcagg tttttaaaaa
    34501 actcggacag gacatacgcc gtcgcattgc cttctgcggt aaaagcggtt gcaataatga
    34561 tttcttcgac ttccccgccg cccagccgtt gcgccagcct gtccaatgcg atggcggata
    34621 cgtccattcc caatgccgta ttgatttgcc ccatcaggac gaaatacagc ccgtcgtggc
    34681 agtttgccgc ttccatattc gacacgtcgg caggcatatg caccaccatc agccgtcgcc
    34741 cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc ttcgcaaaac gtgttgcacc
    34801 tcgcgcaatg atcgacctgc ttcaatgccg tctgcaaggc atccaccagt ttttcagcct
    34861 ctttgcgctt gtgttggagc aaatgatacg ctatccgctg tgccgatttc ggcccgacgt
    34921 tgggtaaaac cttcagcgcg tcgatcaatc cttggaaggc atcttgtttt ttgtggctca
    34981 tcatattccg ccgtatggga aaacggccgg aatattccga ccgttatttt gtcaacaaaa
    35041 gtgtcaatta ctgaccgtcg ccgttgtcga ccgattgcgc tcctttggtc tgtttgattt
    35101 tgccgttgaa ataacgtatc aacaagtcga aagtattggc agactgctgt tgcgccaaag
    35161 cctgttttgc aagcggaagc tgtgcggcga tatcatccgg cggggttaca gcctgtactt
    35221 cgacaatcac gggtgccggc agaccgatca gcctgacgta ggcgggtttg ccgtttgccg
    35281 gttttgcttt cagcagttcc gcataagcct cgggcggcat ggactgcctt gcctgctgtg
    35341 cgcccaaaac ggacacttcc gaccatttca cgtcaacagc cttgccgccg ttcagttggg
    35401 taagcacgtc ttttgccttg ttttcggcaa gtttggcggc ttcggtacgg atataagcct
    35461 gacgtaccgc gtctttggct tcggcaaacg gcagggtttt ctcttcgcgg acttctttgg
    35521 cgcggacgac ccacgcggtt tcgctgttga tggtcagcac ttcggaattg tgttttttct
    35581 tcaatacgtc gtcgctgaat acggcattga tcaggttttc gggcataccg gacatttgcg
    35641 catcctgcct gctcagccaa gtttcttggg tttcgacttt caaaccgctg tttttggcgg
    35701 cttcggcaag cgaggaagga tggttgaacg catcatcgcc cagcttttct tttgccttat
    35761 tgaagtcggc aaccgccttt ttcattttca attcgttttc gacggcggct ttttcctgct
    35821 cgaaagaagg tttggcttca tttgccggca aacgcgccac gcgctcttca aacgcatttt
    35881 tcacttctgt ttcgctgacg gtctgtttgt ctgcaaagtc tttcagattc aaggcgacat
    35941 attccaattt gaccgctttg ggaagcaggt agtctttttt gtttgcgtta taaaacttct
    36001 gcaaatcggc ttcagacact ttgacttggg cgatgaattc gtcggggttg aaagtgtgcg
    36061 aacggatggt gcggttgacc tgcgtcagcc tgatcagctg ttccgcctgc gcgtcgccga
    36121 ccaatacgcc gttttggacg aggtttacca aattctgcaa ggcaaactga tcgcggattt
    36181 cttcgacaaa ctggtcttca gacatatgac gttgggaaag gtagcggttt aaaagcgcgt
    36241 ggtcgaattt gccgtttgcg tcgtggaaat tgggatcgtc cacgataatc tgcttgattt
    36301 gttcggaaga aaccgaaatg cccatcagct tcgcgccctg tttcaggtag gcgcgttgta
    36361 gcagggattg gaacaccgcg tcgcgcgaag ggccgccgcc gtccgcctgt tcgttctgta
    36421 tggcgttgtt gatggagtgg tcgctgattt tttcgtcgcc cacttggacg atgtagtcgg
    36481 cacccggatg ggataccgtg ctgaccccga agccgacgaa ggttaatgca atcaggccca
    36541 aaaggacttg ggcgggcgtt ctgtattttt cgatggaatg gaacatattt taaatcggga
    36601 tatagaatgg gaaattcaag tcgggtattg taacggtttt tatccctgtc tgcacggggc
    36661 ttgccggttg aagatgccgt cgtaggtttc ttcgctgaag ctggcgtaaa ccttgccgcc
    36721 gcactccaat acgggacgct tgatcaggct cggcatttcg gacatcagtt tgacggcctc
    36781 cgccgtcgag gacagcactt tttgctgtgt ttcggcatcg agtttgcgcc agcttgtccc
    36841 gcgtttgttg agcagggttg ccaaaggcac ttgttccagc cacgagcaga tttccgcttc
    36901 agacggcctc tgttttttaa aatcccgaaa ttcaaactcc aagccgtatc cggcaagccg
    36961 gtttttggct tttttgaccg tatcgcaatt tgggatgccg tgaaggatta tcatttggaa
    37021 accttttgtc tgaaataata aaacggatat tttactataa gtgtctgaaa atttgcccgt
    37081 ctgtttcaga cggcggggcg gttatgttac aatccgaaaa ttcgaaaaat ttaatctctt
    37141 gttcaataaa ggctttacca atcatgattt ctaccaacgg catcaccatg cagttcggcg
    37201 cgaagccgct gtttgaaaac gtatccgtta aattcggcga aggcaaccgc tacggtttga
    37261 tcggcgcgaa cggctcaggc aaatccacct tcatgaaaat cctcggcggc gatttggaac
    37321 agacagccgg cgaagtggcg attgaaaacg gcgtgcgttt gggtaaattg cgccaagacc
    37381 agtttgccta cgaagatatg cgcgtgctgg acgtggtaat gatgggtcac accgaaatgt
    37441 gggcggcgat gaccgaacgt gatgcgattt acgccaatcc cgaagccacc gaagacgact
    37501 acatgaaagc cgcagaactg gaagccaagt tcgccgaata cgacggctac accgccgaag
    37561 cgcgtgccgc cgagttgttg agcggcgtgg gcatttccga agatttgcac aatgcaacca
    37621 tggcagaagt tgccccgggc ttcaaactgc gcgtattgct ggcgcaagcc ctgttctcca
    37681 agccggatgt attgctcttg gacgaaccga ccaataactt ggacattaat accatccgtt
    37741 ggttggaagg cgtgttgaac cagtacgact ccacgatgat tatcatctcg cacgaccgcc
    37801 acttcttgaa cgaagtctgc acgcatatgg cagatttgga ttacaacacc atcaccatct
    37861 atccgggcaa ctacgacgac tatatgctcg cctccgccca atcgcgcgaa cgcgccctga
    37921 aagacaatgc caaggcgaag gaaaaattgc aggaattgca agaatttgtg gcgcggttca
    37981 gtgccaacaa atccaaagcc cgtcaggcaa ccagccgtct gaaacaggca gacaaaatca
    38041 aatcggagat ggtcgaggtc aaaccctcta cccgtcaaaa cccgtatatc cgttttgaag
    38101 ccgatgaaaa agccaagctg caccgtcagg ctgtggaagt tgaaaaactg gcgaaacgct
    38161 ttgaaaccca gttgtttaaa aacctgaact ttatccttga agcgggacaa cgcctcgcca
    38221 tcatcggccc gaacggcgcg ggcaaatcca ccctgctgaa actcttggcc ggcgcgtaca
    38281 accccgaata ttcagacggt ctgctgccgg acgaaggcag catcaaatgg gcggaaaaag
    38341 ccagcgtcgg ctactatccg caagatcacg aaaacgactt cgatgtcgat atggatttga
    38401 gcgaatggat gcgccaatgg gggcaggacg gcgacgacga acaagtcatc cgcggcactt
    38461 tggggcgttt gctcttcggc agtaacgatg tcgtgaaaaa agtgaaggtt ctctccggtg
    38521 gtgaaaaagg ccgtatgctt tacggcaaac tgttgctgtt gaaacccaat gtcttagtca
    38581 tggatgaacc gaccaaccac atggacatgg aaagcatcga atccttgaac atggcgctgg
    38641 aaaaatacaa cggcacgctg attttcgtct cccacgaccg tcagttcgtt tcttccctgg
    38701 ctacccaaat cattgaattg gacggcaaag gcggatatga acactacttg ggcgattacg
    38761 aaagctatct cgagaaaaaa ggcgtagcat aaccgccggt tggaacaatg ccgtctgaag
    38821 cggcttcaga cggcattgtt gataacttta aaataggaag catatgcaga cttatctcgt
    38881 cggcggtgcc gtccgcgatt atcttttggg cttgcccgtc aaagaccgcg attgggtggt
    38941 cgtcggcgca gacgcacaaa ccatgctggc gcaaggcttc cagccagtcg gcaaagactt
    39001 tcccgtgttt ctccatcccg aaacacacga agaatacgcc ctcgcccgca ccgagcgcaa
    39061 aaccgccaaa ggttacgtcg gtttcagttt ccacgccgac aaagacgtta cgctggagca
    39121 ggatttgatg cgccgcgacc tgaccatcaa cgcgatggcg caagatgcgg acggcaagat
    39181 tatcgaccct ttcggcggac aacgggattt ggcggcaggc attttgcgcc acgtttcccc
    39241 agcctttgcc gaagaccccg tccgcatcct gcgtactgcc cgctttgccg cgcgttacaa
    39301 gtttgaaatc gccgaagaaa ccataaagct gatgcggcag atggtggaaa acggcgaagc
    39361 ggacgcattg gttgccgaac gcgtctggca ggagtttgcg aaaggtttga tggaaaaaaa
    39421 tccgcgcaaa atgattgaag tgttgcgcga atgcggcgcg ctcaaagtct tgctgcccga
    39481 agtcaatgcc ctcttcggcg tgccgcaacg cgccgactac catcccgaaa tcgacagcgg
    39541 catccatacc ctgatgacgc tgcaacgcgc cgccgatatg ggtttgagcc tgcccgaacg
    39601 ctatgccgcc ctgctgcacg acttgggcaa agccaaaaca ccgtccgaca tcctgccgcg
    39661 ccaccacgga cacgacctcg ccggtgtcga acccgtgcgc aaagtcaatc aaaggctgcg
    39721 tgcgccgaaa cattgtgccg agcttgccga attggtttgc cgttggcaca ttattttcca
    39781 ccaagtcgga cagcttaaaa gccaaaccat tctgaacgta ttgaaaaaaa ccgacgcttt
    39841 cagacgaccc gaacgctttc agacggcatt gaacgtctgc attgccgaca cgcaaggccg
    39901 tctgaaccgc gaacacacgc cctacccgca acgcgcgcac tggctcgcct tactcgaagc
    39961 cgccaatcag gcggattcgg gcaaaatcgc cgccgaatgc cgctcgcagg gaaaagcgca
    40021 ccttatcgcc gaacaaatcg accgggcgcg gctggcacaa atcgccccat tgcaaaaagc
    40081 gtttcgagcg gcgcaagaca aaacagaaaa acattaaaac gtccaatgca gccactttta
    40141 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
    40201 caaggcgagg caacgctgta ctggtttttg ttaatccact ataaagtttt gaggacgata
    40261 cccaatccaa gctttgcaac agccgccgcc atatccgcta taattcacgc ttcagccatt
    40321 ccgcccccga cataaaatca tgaccctgaa aaccgattta ttgcctaaaa tcaacaacga
    40381 agattatcaa cgcctcatcc tcaaacacag tgcggaattt agcggtggcg aaatccgcct
    40441 attgaacgaa atcctcgaaa aattcaattt cgacgttgtt caggcgcagg cgttggcgca
    40501 ggcggtgatg cagcaagtcc gcttcgaccc caacgcctat cacatcgaca gcgacgacga
    40561 agacaccaca ggcatctgcc cacactgcat caacccgcct atgccgcccc tgcgcgacta
    40621 tctcgtttgg cgcgaaacgc gcgggtaaaa cgcttttgac cgttatcttt tcaatgccgt
    40681 ctgaaacgcc gccgaccgtt cagacggcat acccgacaaa gggaacacta tgctgcaaac
    40741 cgacaacctg actgccgctc aaccgcaacg cattgttgcc gcccaaaccg cctccgcaca
    40801 ggaagaactg ctcgaacgcg ccctccgccc caaaacgctg gacgactata tcgggcagca
    40861 taaagccaaa gaacagctcg ccattttcat ccaagccgcc aaaaagcgcg gcgaagcact
    40921 cgaccacgtc ttgcttttcg gtccgcccgg gctgggtaaa accacactgg cgcacatcat
    40981 cgccaaagaa ttgggcgtaa atttgcgcca aaccagcggc cccgtcctcg aacgcgccgg
    41041 cgacctcgcc gcccttttga ccaaccttga tccgcacgac gtattgttta tcgacgaaat
    41101 ccaccgcctc agccctgttg tcgaagaaat cctctacccc gcgcttgaag actaccggct
    41161 cgacattatg ataggcgaag ggcccgccgc ccgttccgtt aaaatcgacc tgccgccctt
    41221 cacgcttgtc ggcgcgacca cccgcgccgg tatgctgacc aatccgttgc gcgaccgctt
    41281 cggcatcgtc tcccgcctcg agttttacga aaaccgcgac cttgccacca tcgtcagccg
    41341 ttcggcacaa ctcctgcaac tcgatatgtc cgaagaaggc gcggaagaaa tcgccaaacg
    41401 cagccgcggt acgccgcgca tcgccaaccg cctgttgcgc cgcgtgcgcg atttcgccga
    41461 cgtgaaaaac aacggcacaa tcgacggcgg catcgccgat gccgctttaa gtatgctgga
    41521 cgtggacgtg caggggctgg acgtgatgga caggaaattc ctcgaagccg ttttgcacaa
    41581 attcggcggc ggcccggtcg gtttggacaa tgttgccgct gccatcggcg aatcaacaga
    41641 caccatcgaa gacgttatcg aaccctatct catccaacaa ggcttcctgc aacgcacccc
    41701 gcgcggcagg atggcgaccg aacgcgccta cctgcatttc gggctgcccg tcgaaaaata
    41761 acgcaatgcc gtctgaaaca gagctaattt tcagacggca tttctatttc aatcattggc
    41821 gcaaggttca gcctgccgct tttttccagt tccgccctca tcgcatcaat caccgcctta
    41881 tagtctggtt tgccgaaaat cgcagaaccg gcaacaaagg tatccgcgcc ggcttgggcg
    41941 gcggcggcga tattgtcggt tttgatgccg ccgtccactt caatggcaat acgtctgccg
    42001 cttttctctt catagcggtc aagcatggca cgaacctgtc tgattttttc caaagtatac
    42061 ggaatgaagc tctgaccgcc gaacccgggg tttaccgaca tcagcaaaac catatccagc
    42121 ctgtccaata cgttttccaa cagatatacg ggcgttgccg gattcaacac cagccccgcc
    42181 tgacagccca tgtcccgaat caggctcaag ctgcggtcaa tatggcggct cgcttcggga
    42241 tggaacgtga tgattgatgc tcctgctttg gcaaacgact gaatcaggtc atccacgggt
    42301 tcgaccatca gatgcacatc gatcggcacg cttgcataag gcttcaacgc cgcgcaaacc
    42361 ataggcccga aggtcaggtt cggcacataa tggttgtcca tcacgtcaaa atggatcaga
    42421 tctgcacctg ccgcaatgac gctttccacc tcttcgccaa gccgtgcaaa atctgccgat
    42481 aaaatgctgg gtgcgatacg gtaagtagtc atgttttttt ccttcaatat cctt
//
LOCUS       DAWFIT010000006        40870 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFIT010000006 DAWFIT010000000
VERSION     DAWFIT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.006"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(171..863)
                     /locus_tag="WGQ85_000284"
     CDS             complement(171..863)
                     /locus_tag="WGQ85_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0749790.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGI
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WGQ85_000285"
     CDS             complement(971..2164)
                     /locus_tag="WGQ85_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0749791.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGQ85_000286"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WGQ85_000286"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0749792.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WGQ85_000287"
     CDS             complement(3017..3946)
                     /locus_tag="WGQ85_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0749793.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGQ85_000288"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WGQ85_000288"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0749794.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WGQ85_000289"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WGQ85_000289"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0749795.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7216..7674
                     /locus_tag="WGQ85_000290"
     CDS             7216..7674
                     /locus_tag="WGQ85_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0749796.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(7945..8160)
                     /locus_tag="WGQ85_000291"
     CDS             complement(7945..8160)
                     /locus_tag="WGQ85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749797.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(8168..8371)
                     /locus_tag="WGQ85_000292"
     CDS             complement(8168..8371)
                     /locus_tag="WGQ85_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749798.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WGQ85_000293"
     CDS             complement(8284..8643)
                     /locus_tag="WGQ85_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0749799.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8696..9043
                     /locus_tag="WGQ85_000294"
     CDS             8696..9043
                     /locus_tag="WGQ85_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0749800.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            9271..9891
                     /locus_tag="WGQ85_000295"
                     /pseudo
     CDS             9271..9891
                     /locus_tag="WGQ85_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
     gene            9914..11365
                     /locus_tag="WGQ85_000296"
     CDS             9914..11365
                     /locus_tag="WGQ85_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ0749801.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11827..12183
                     /locus_tag="WGQ85_000297"
     CDS             11827..12183
                     /locus_tag="WGQ85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0749802.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12174..12656
                     /locus_tag="WGQ85_000298"
     CDS             12174..12656
                     /locus_tag="WGQ85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0749803.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12669..13262
                     /locus_tag="WGQ85_000299"
     CDS             12669..13262
                     /locus_tag="WGQ85_000299"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0749804.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13252..14508
                     /gene="nuoD"
                     /locus_tag="WGQ85_000300"
     CDS             13252..14508
                     /gene="nuoD"
                     /locus_tag="WGQ85_000300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0749805.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDVFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14508..14981
                     /gene="nuoE"
                     /locus_tag="WGQ85_000301"
     CDS             14508..14981
                     /gene="nuoE"
                     /locus_tag="WGQ85_000301"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0749806.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            15371..16672
                     /gene="nuoF"
                     /locus_tag="WGQ85_000302"
     CDS             15371..16672
                     /gene="nuoF"
                     /locus_tag="WGQ85_000302"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0749807.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            16672..17109
                     /locus_tag="WGQ85_000303"
     CDS             16672..17109
                     /locus_tag="WGQ85_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749808.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            17399..19660
                     /locus_tag="WGQ85_000304"
     CDS             17399..19660
                     /locus_tag="WGQ85_000304"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0749809.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAMGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWADRLKKLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQATVH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLSLHTENAA
                     LGALMDTIELAGA"
     gene            19663..20739
                     /gene="nuoH"
                     /locus_tag="WGQ85_000305"
     CDS             19663..20739
                     /gene="nuoH"
                     /locus_tag="WGQ85_000305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0749810.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            20819..21298
                     /gene="nuoI"
                     /locus_tag="WGQ85_000306"
     CDS             20819..21298
                     /gene="nuoI"
                     /locus_tag="WGQ85_000306"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0749811.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            21329..22036
                     /locus_tag="WGQ85_000307"
     CDS             21329..22036
                     /locus_tag="WGQ85_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749812.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            22072..22743
                     /locus_tag="WGQ85_000308"
     CDS             22072..22743
                     /locus_tag="WGQ85_000308"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0749813.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            22740..23045
                     /gene="nuoK"
                     /locus_tag="WGQ85_000309"
     CDS             22740..23045
                     /gene="nuoK"
                     /locus_tag="WGQ85_000309"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0749814.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            23079..23654
                     /locus_tag="WGQ85_000310"
     CDS             23079..23654
                     /locus_tag="WGQ85_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0749815.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            23778..24119
                     /locus_tag="WGQ85_000311"
     CDS             23778..24119
                     /locus_tag="WGQ85_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749816.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            24168..26192
                     /gene="nuoL"
                     /locus_tag="WGQ85_000312"
     CDS             24168..26192
                     /gene="nuoL"
                     /locus_tag="WGQ85_000312"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0749817.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLTSPVLYLAAAGVAAAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26260..26580
                     /locus_tag="WGQ85_000313"
     CDS             26260..26580
                     /locus_tag="WGQ85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0749818.1"
                     /translation="MSNTQTIRSADFTASRAWGALDIANMNGTTVRLHWTDQSYKWHV
                     NDGEEVFAVMNGEVDMHYRENGEEHIVRLKSGDIFYAGIGTEHVAHPCGEARILVIEK
                     EGSA"
     gene            26639..27802
                     /locus_tag="WGQ85_000314"
     CDS             26639..27802
                     /locus_tag="WGQ85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0749819.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIQEVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKAKSWANEIVMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            27917..29413
                     /locus_tag="WGQ85_000315"
     CDS             27917..29413
                     /locus_tag="WGQ85_000315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0749820.1"
                     /translation="MFSNYLLSLAIWIPIVAGVLVLATGPDSRAPFARVLALIGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQLPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            29423..30868
                     /gene="nuoN"
                     /locus_tag="WGQ85_000316"
     CDS             29423..30868
                     /gene="nuoN"
                     /locus_tag="WGQ85_000316"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0749821.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSSFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            30980..31279
                     /locus_tag="WGQ85_000317"
     CDS             30980..31279
                     /locus_tag="WGQ85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0749822.1"
                     /translation="MTASMYILLVLALIFANVPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRVGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(31388..32284)
                     /locus_tag="WGQ85_000318"
     CDS             complement(31388..32284)
                     /locus_tag="WGQ85_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0749823.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTAILGKTAGKDADNDKPTYVRLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(32268..32492)
                     /locus_tag="WGQ85_000319"
     CDS             complement(32268..32492)
                     /locus_tag="WGQ85_000319"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0749824.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(32490..32579)
                     /locus_tag="WGQ85_000320"
                     /pseudo
     CDS             complement(32490..32579)
                     /locus_tag="WGQ85_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(32606..33529)
                     /gene="rsgA"
                     /locus_tag="WGQ85_000321"
     CDS             complement(32606..33529)
                     /gene="rsgA"
                     /locus_tag="WGQ85_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0749825.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETHALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(33534..35390)
                     /locus_tag="WGQ85_000322"
     CDS             complement(33534..35390)
                     /locus_tag="WGQ85_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0749826.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPTELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(35416..35553)
                     /locus_tag="WGQ85_000323"
     CDS             complement(35416..35553)
                     /locus_tag="WGQ85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749827.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(35553..36137)
                     /gene="ruvA"
                     /locus_tag="WGQ85_000324"
     CDS             complement(35553..36137)
                     /gene="ruvA"
                     /locus_tag="WGQ85_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0749828.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(36219..36545)
                     /locus_tag="WGQ85_000325"
     CDS             complement(36219..36545)
                     /locus_tag="WGQ85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0749829.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(36599..37318)
                     /locus_tag="WGQ85_000326"
     CDS             complement(36599..37318)
                     /locus_tag="WGQ85_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0749830.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVGQGTAHVKIEQIVPGQSTPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            complement(37315..37437)
                     /locus_tag="WGQ85_000327"
     CDS             complement(37315..37437)
                     /locus_tag="WGQ85_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0749831.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(37448..37912)
                     /gene="trmL"
                     /locus_tag="WGQ85_000328"
     CDS             complement(37448..37912)
                     /gene="trmL"
                     /locus_tag="WGQ85_000328"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0749832.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(37977..38474)
                     /locus_tag="WGQ85_000329"
     CDS             complement(37977..38474)
                     /locus_tag="WGQ85_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0749833.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLTD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            38530..39444
                     /gene="bioH"
                     /locus_tag="WGQ85_000330"
     CDS             38530..39444
                     /gene="bioH"
                     /locus_tag="WGQ85_000330"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0749834.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            39446..40234
                     /locus_tag="WGQ85_000331"
     CDS             39446..40234
                     /locus_tag="WGQ85_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0749835.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            40511..>40870
                     /locus_tag="WGQ85_000332"
     CDS             40511..>40870
                     /locus_tag="WGQ85_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749836.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQQYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKVAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT         9474 a        10255 c        10936 g        10205 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatatgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg
     4921 cttttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc
     5101 ggtttgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc
     5521 ggcgacgacg accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg
     5581 ctgatggaag agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc
     6001 ctcttgcgcg tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt
     6061 cagacggcct caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca
     6121 aaagccacca aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac
     6241 tacaaaaccc aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca
     6361 gacgaccccg cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc
     6481 ttggaattta acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcacagcaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gaaatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt
     6961 gcgaaattgg aagcgatgta gaagggagat ggcagaggcc gtctgaaaac gggaacaaca
     7021 atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 gggaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactacaaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 aaagatcatg tgcaaatttg cgaggtatcg ggaatcggtt ttgaaaaaga aggaagcctg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg agtatccgcc catcggtgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccctatttg ccttagacta tcgggagtgt
     7621 ggaagagacg gagagccgaa agtggtgttt gtcgaacaag aatcggattt ttaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatt cacagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accagaatca tcaaaggaaa acgggcggaa
     7801 taacagataa ccgattgaat ccgtaaggaa aacggtttgt aggaacgtgc catccaagac
     7861 ctttgcaaag aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt
     7921 ggagctatct caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat
     7981 taaatgttgt tctagctctt gcactgattg aggattgcga attcctgata caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata aatagggaaa ttcaaattaa gttagaatta tccctatgag
     8701 aaaaagtcgt ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgcaggtgt
     8761 aactgcaaga acagcaacag agcctgacag cattgtttat acggattgtt atcgtagcta
     8821 tgatgtatta gatgtgagcg aatttagcca tttccgtatc aatcacagca cacattttgc
     8881 tgaacgacaa aaccatatca atggaattga gaatttttgg aaccgggcaa aacgtcattt
     8941 acgcaagttt aacggcattc ccaaagcgca ttttgagctg tatttaaagg agtgcgaatg
     9001 gcgttttaac aacagtgaga taaaagttct tgttccattt taaaacaatt agtaaaatcg
     9061 agtttatctt agttgtccag gacagcccca ttatttttat aacaccgtga agccgcacag
     9121 cagtttgaac agtgatacgc cgtttgcggg cttacgagtt tattttcccg gcctgcagtt
     9181 tgagcaatac ggtgatttcc tacggttaat acaaatgttt acacattgat acatttcatt
     9241 tatagttccg cctatttgaa aatagaaaat atgaattcga ccgcaagtaa aaccctgaaa
     9301 ggattgtcgc tggtgttttt cgcctctgga ttctgcgccc tgatttacta ggtcagctgg
     9361 cagaggcttc tattcagtca cataggtatc gatttaagtt cgattactgt cattatttct
     9421 gtatttatgg tcggcttggg tgtaggtgcg tatttcggtg gacgcattgc tgaccgtttt
     9481 ccttcaagta tcatccccct gttttgcatc gctgaagtat ccatcggtct gttcggtttg
     9541 gtaagcaggg gtctgatttc cggcttgggg catcttttag ttgaggctga tttgcccatc
     9601 atcgctgctg ccaatttcct cttattgctg cttcctacct ttatgatggg cgcgaccttg
     9661 cccttgctga cctgtttttt taaccggaaa atacataatg ttggcgagtc tatcggtacc
     9721 ttatattttt tcaacacttt gggtgcggca ctcggatcgc ttgccgccgc cgaatttttc
     9781 tacgtctttt ttaccctctc ccaaaccatt gcgctgacag cctgctttaa ccttctgatt
     9841 gctgcttcag tatggctgcg ttacagaaag gatggatata gtgaacacta aaccgaatac
     9901 tagtttgatt tatatgcttt ctttccttag cggcttattg agcttgggta tagaagtctt
     9961 gtgggtaagg atgttttcgt tcgcagcaca gtccgtgcct caggcatttt catttactct
    10021 tgcctgtttt ctgaccggta tcgccatcgg cgcgtatttt ggcaaacgga tttgccgcag
    10081 ccgctttgtt gatattccct ttatcgggca gtgcttcttg tgggcgggta ttgccgactt
    10141 tttgattttg ggtgctgcgt ggttgttgac gggtttttcc ggcttcgtcc accacgccgg
    10201 tatcttcatt accctgtctg ccgtcgtcag agggttgatt ttcccgctcg tacaccatgt
    10261 gggtacggat ggcaacaaat ccggacgaca ggtttccaat gtttatttcg ccaacgttgc
    10321 cggcagtgca ttgggtccgg tccttatcgg ctttgtgata cttgatttct tgtccaccca
    10381 acagatttac ctgctcatct gtttgatttc tgctgctgtc cctttgtttt gtacactgtt
    10441 ccaaaaaagt ctccgactga atgcagtgtc ggtagcagtt tccctaatgt tcggcatcct
    10501 catgttccta ctgccggatt ctgtctttca aaatattgct gaccgtccgg ataggctgat
    10561 tgaaaacaaa cacggcattg ttgcggttta ccatagagat ggtgataagg ttgtttatgg
    10621 ggcgaatgta tacgacggcg catacaatac cgatgtattc aatagtgtca acggcatcga
    10681 acgtgcctat ctgctaccct ccctgaagtt tggcatacgc cgcattttcg tcgttggatt
    10741 gagtacaggt tcgtgggcgc gcgtcttgtc tgccattccg gaaatgcagt cgatgattgt
    10801 tgcggaaatc aatccggcat accgtagcct tatcgcggac gagccgcaaa tcgccccgct
    10861 tttgcaggac aaacgtgttg aaattgtatt ggatgacggt aggaaatggc tgcgtcgcca
    10921 tcctgatgaa aaattcgacc tgattttgat gaatacgact tggtactggc gtgcctattc
    10981 caccaacctg ttgagtgcgg aatttttaaa acaggtgcaa agccacctta ccccggatgg
    11041 tattgtaatg tttaatacca cgcacagccc gcatgctttt gctaccgccg tacacagtat
    11101 tccctatgca taccgctatg ggcatatggt agtcggctcg gcaaccccgg tagttttccc
    11161 taataaagaa ctgctcaagc aacgtctctc ccggttgatt tggccggaaa gcggcaggca
    11221 cgtatttgac agcagcaccg tggatgctgc agcacaaaag gttgtctctc gtatgctgat
    11281 tcagatgacg gaaccttcgg ctggggcgga agtcattacc gacgataata tgattgtaga
    11341 atacaaatac ggcagaggga tttaaccgtc ttaaagggtt tcaggcaacg caggttttag
    11401 gtaacgtcct gctagttcaa aaaaaccgca tcacagcagt cgggacaaaa tggtttaaac
    11461 attttgtccc gaattcttat tcctatatat agtggattaa caaaaatcag gacaaggcga
    11521 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag
    11581 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
    11641 taccacgaat tacggtgtaa aaatttatat gaccttataa aatcaaataa gaatcgttat
    11701 cataacatga ttgtatttat tgggtttttt tgggcgtttt gccgatattt accttttaat
    11761 ggtttttgaa attcgctaaa atacgaaatt attgtagaaa ttttgttaac ggatttgggt
    11821 gtaaccatgt tgtccgctta ctttcccgtc tttgtcttta tcctcgtcgg cctcgcggcc
    11881 ggcgtgctgt ttatcctgct cggcacgatt ttgggcccga aacgccacta cgccgaaaaa
    11941 gacgcgcctt acgaatgcgg ttttgaggcc tttgaaaacg ccaggatgaa gttcgacgtg
    12001 cgctattacc tcgtcgccat cctctttatc ctgtttgatt tggaggtcgc gttcatgttg
    12061 ccgtgggcag ttgtgttcaa agatttgggt gcgtacggct tctggtctat gctggtgttt
    12121 atcgtcgttc tgacggtagg ctttgtttac gaatggaaaa aaggtgcgct ggaatgggaa
    12181 tagaaggcgt tttgaaaaaa ggtttcatca ccaccagcgc ggatacggtg ttgaactata
    12241 tgcgtaccgg ttcattgtgg ccggttactt tcggcttggc ctgctgcgcc gtggaaatga
    12301 tgcatgcggg tatggcgcgt tatgaccttg accgcttcgg catcattttc cgtccgtccc
    12361 cacgtcaggc cgacctgatg attgtggcag gtacgctgac caacaaaatg gcgcccgccc
    12421 tgcgccgcgt atacgaccag ctcgccgagc cgcgctgggt attgtctatg ggctcgtgtg
    12481 ccaacggcgg cggctattat cactattctt attccgttgt gcgcggtgcc gaccgcgtcg
    12541 tgccggtaga cgtttatgtg ccgggttgtc cgccgactgc ggaagccctg atttacggtc
    12601 tgattcagct ccaacaaaaa atcaagcgca cttccaccat cgcgcgtgac gagtaaggag
    12661 aggacgatat ggcaagcatt caaaacttat acgaaaccgt cagccgcgtt ttgggcaatc
    12721 aggcaggcaa agtcatttcc gctttgggcg agattaccgt cgagtgtctg cccgaacact
    12781 atatttcagt catgaccgca ttgcgcgacc atgaagagtt gcatttcgag cttctggtcg
    12841 acttgtgcgg tgtcgattac agcacttaca aaaacgaagt atggcagggc aaacgctttg
    12901 ccgtcgtcag tcagttgctt tccgttaaaa acaatcaacg catccgcgta cgcgtctggg
    12961 tttcagacga cgacttccct gtagtcgaat ccgtagtcga tatttacaac agcgcggatt
    13021 ggtacgaacg cgaagccttc gatatgtacg gcatcatgtt caacaaccac ccggacttgc
    13081 gccgcatcct gaccgattac ggctttgtcg gacatccgtt ccgcaaagac ttcccgattt
    13141 ccggctatgt ggaaatgcgt tacgacgaag agcaaaaacg cgtgatttac caacctgtta
    13201 ccattgagcc gcgcgagatc acgccgcgta tcgtccgtga ggagaactac ggtggccaat
    13261 aaattaagaa actacaccat caacttcggc ccgcaacacc ctgcggcgca cggcgtattg
    13321 cgtatgattt tggagctgga cggcgaacaa atcgtccgtg ccgacccgca tatcggcctc
    13381 ctgcaccgag gcacagagaa actggcggaa accaaaacct acctgcaagc cctgccttat
    13441 atggacaggc tggactacgt ttccatgatg gtcaacgagc aggcgtattg cttggcggta
    13501 gaaaaacttg ccggtatcga tgtgcctatc cgcgcccaat acatccgcgt gatgtttgcc
    13561 gaagtaacgc gcatcctcaa tcacttgatg ggcatcggtt cgcatgcctt cgacatcggc
    13621 gcgatgaccg ccattcttta cgccttccgc gaccgtgaag agctgatgga cttgtacgaa
    13681 gccgtttccg gcgcgcgtat gcacgccgcc tacttccgtc ccggcggcgt ttaccgcgac
    13741 ctgcccgact tcatgcctaa atacgagggc agcaaattcc gcaatgccaa agtattgaag
    13801 cagctcaacg aatcccgcga aggcacgatg ctcgacttta tcgatgtctt ctgcgaacgt
    13861 ttcccgaaaa atatcgacac actcgaaacc ctcctgaccg acaaccgtat ttggaaacag
    13921 cgtaccgtcg gcatcggcgt cgtctccccc gaacgtgcca tgcaaaaagg ctttaccggc
    13981 gtgatgttgc gcggttcggg tgtggaatgg gacgtgcgta agaaacagcc ttacgaagtg
    14041 tacgacaaaa tggatttcga catccctgtc ggcgtgaacg gcgactgcta cgaccgctac
    14101 ctctgccgta tggaagaaat gcgccaatcc gtacgcatca tcaaacaatg ctccgagtgg
    14161 ttgcgtgtca atccgggtcc ggtcattacc acaaaccaca aattcgctcc gcccaaacgt
    14221 accgaaatga aaacaggtat ggaagacctg attcaccatt tcaaactctt taccgagggt
    14281 atgcacgttc ccgagggcga gacttacacc gctgtcgaac atccgaaagg cgagttcggc
    14341 gtttacatca tttcagacgg cgcgaacaaa ccctaccgcc tgaaaatccg cgcacccggc
    14401 ttcgcccatc tgcaaggtat ggacgaaatg gcaaaaggcc atatgctcgc cgacgtcgtt
    14461 gccatcatcg gtacgcagga catcgtattc ggggaggttg accgataatg ttatccgcag
    14521 aatccttaaa acaaatcgac atcgagttgg cgaaatatcc cgccgaccaa cgccgctctg
    14581 cgattatggg cgcattgcgt attgcccaga ccgaaaaagg ctggctcgcc cccgaaacca
    14641 tcgcttttat cgccgactac atcggcatct cgcctgcaca agcctacgaa gtcgctacct
    14701 tctacaatat gtacgacctt gagcctgtcg gcaaatacaa actgaccgtt tgtaccaacc
    14761 tgccctgcgc cctacgcggc ggtatggcga ccggcgaata cctcaaacaa agactcggta
    14821 tcggctacgg cgaaaccacg cccgacggca aatttaccct tgtcgaaggc gaatgcatgg
    14881 gcgcgtgcgg cgacgctccg gttatgctgg tcaacaacca cagcatgtgc agctttatga
    14941 ccgaagaggc gattgagaag aaactggcgg agttggagta ggtcgtctga aacgacgatt
    15001 taaacgtagg tcggatactt gtatccgaca gagtgggtaa aaaggcaaaa tgtcggattt
    15061 aagaatccgc cctactgaaa taccgaaatg ccgtcattcc cgcgcaggcg ggaatccacc
    15121 ggtaagattc ggtttctgaa tttaataaga cattgcttac cattgaggat ggattcccgc
    15181 ctgcgcggga atgacgacag acaagcaagt ggtcgagatc caacaaaaac gattaaaggt
    15241 cgtctgaaaa tatcgatttg ataaactaga ttttatttca gacgacgtta caagccggta
    15301 cacaaagaca tcttaaggtc gtctgaaaca gcggccgcaa ccgatacgaa aacaaacagg
    15361 cacaccaaaa atggctattt accaatcagg cgtgattttt gaccaagtgg ataccgccaa
    15421 tcccgactgc tggacattgg acgaatacgt caaacgcggc ggctataccg ccctgcgtaa
    15481 aattctgtcc gaaaacatct cgcaaaccga tgtgattgac gaagtcaaaa cctccggctt
    15541 gcgcgggcgc ggcggtgcgg gcttcccgac tggtttgaaa tggagcttta tgccccgttc
    15601 cttcccgggc gaaaaatatg tggtttgtaa caccgacgaa ggcgaaccgg gtacatttaa
    15661 agaccgtgac atcatcatgt tcaatcctca tgccctgatt gaaggcatga ttatcgccgg
    15721 ttacgcgatg ggtgcgaaag ccggctacaa ctatatccac ggcgaaatct tcgaaggcta
    15781 ccaacgcttt gaagccgctt tggagcaggc gcgtgccgca ggctttttgg gtaaaaatat
    15841 tttgggttcg gattttgaat ttgaactctt cgcccaccac ggctacggcg catatatttg
    15901 cggcgaagaa accgcattgc tcgaatcgtt ggaaggcaaa aaaggccagc cgcgcttcaa
    15961 accgccattc cctgcttcgt tcggcctgta cggcaaaccg accaccatta ataatactga
    16021 aaccttttcc tccgtcccat tcattgtccg tgatggcgga caggcatttg ccgataaagg
    16081 tattccgaat gcaggcggta ccaaattgtt ctgtatttcc ggccatgtcg agcgtccggg
    16141 caactatgaa gtgccattgg gcacaccgtt tgccgaagtc ttgaaaatgg caggcggtat
    16201 gcgcggcggc aaaaaactca aggccgttat tcccggcggt tcgtccgcgc ccgtgttgcc
    16261 tgccgatatc atgatgcaga ccaatatgga ctatgactcg atttctaaag caggctcgat
    16321 gttgggttcc ggcgcgatta tcgtcatgga cgaagatgtg tgcatggtca aagcccttga
    16381 acgcctgagc tacttctact atgacgagtc ttgcggccaa tgtacgcctt gccgagaagg
    16441 tacgggctgg ctctaccgca tcgtccaccg catcgtagaa ggcaaaggca aaatggaaga
    16501 tttggatttg ctggattccg tcggcaacca aatggcaggc cgcaccatct gcgcccttgc
    16561 cgatgccgcc gttttccctg ttcgcagctt taccaagcat ttccgtgatg agtttgtgca
    16621 ttacatcgaa cacggcggac cgatgaaaga gcataagtgg ggagggtggt aatggtggaa
    16681 gctaaaattt ttattctata cggtgcagcc aacaaaggta agagtacgac actcaatacg
    16741 ctttttaatc agatttgttg gaaattttct aaatttctag tcttttttga aagacatgga
    16801 aacggcttag attttgttgc agtatttgat catgaaggtc agaggattgg tttttattca
    16861 tctggtgata atgaatacga ggttaggaga aatttataca aactttattc gcataattgt
    16921 gattttattt ttggcacgtc aaggacacgg ggtggtagtt gcgatgcagt aggatgttat
    16981 gcagagttat tgcatggcga tgtaaatata attaattggt gtgaaaagtt tgagcctaca
    17041 gatgaagaca atgagcgtgc tgttaaagag ttatttaagt catttaaaaa tataataaat
    17101 gagttatagt tttagttggt tttatattgg ttaaaagcaa aatgctaaaa atttaacttt
    17161 gccgtcattc ccgcgtaggc gggaatccat agtggaattt acagaacccg atatttgaaa
    17221 agcagttacc gaaaacccaa gaatggattc ccgcctgtgc gggaatgacg gcaaggtggc
    17281 agtaaacgtt ttaaacagta ttgattgtca atgaaactca aaaggccgtc tgaaacccat
    17341 ttttcagacg atctccataa agaattattt atcaaatacc cgtaactagg aacgaaccat
    17401 gttacaaatc gaaatcgacg gcaaacaagt atctgtggag cagggcgcga cggtgattga
    17461 agccgcgcac aagctcggta cttatatccc gcatttctgt taccacaaaa aactttccat
    17521 cgccgccaac tgccgtatgt gtttggtgga cgtggaaaaa gcccccaaac ctctgccagc
    17581 ctgtgccacg ccggtaacgg acggcatgat tgtgcgtacg cattcggcaa aagcccgaga
    17641 ggcgcaagaa ggcgtgatgg agttcctgct catcaaccat ccgcttgatt gtccgacctg
    17701 cgaccagggc ggcgaatgcc agttgcagga tttggcgatg ggctacggca aaaccaccag
    17761 ccgttatacc gaagaaaaac gttccgtcgt cggcaaagat atgggacctt tggtttccgc
    17821 cgaggaaatg agccgctgca tccattgcac ccgctgcgtg cgattcaccg aagaaattgc
    17881 cggtttgcag gaaatcgcga tggcgaatcg cggcgaacac tccgaaatca tgccttttat
    17941 cggcaaagca gtggaaaccg aattgtcagg caacgtcatc gatttgtgtc ctgtcggtgc
    18001 attgaccagc aaaccgttcc gcttcaacgc gcgtacttgg gagctgaacc gccgcaaatc
    18061 cgtttctgcc catgatgctt tgggcagcaa tttgattgtg caaaccaaag accataccgt
    18121 ccgccgcgtg ctgccgttgg aaaacgaagc gattaacgaa tgctggctgt ccgaccgcga
    18181 ccgtttcgcc tacgaaggcc tgtatcacga aagccgtctg aaaaacccga aaatcaaaca
    18241 gggcggcgag tggatggacg tggattggaa aaccgcgttg gaatatgtcc gcagcgcgat
    18301 tgaatgtatc gccaaagacg gtaaccaaaa ccaagtcggc atttgggcga acccgatgaa
    18361 tacggttgaa gagctgtatc tggcgaaaaa actcgccgac ggcttgggtg ttaaaaactt
    18421 tgccacccgt ttgcgccaac aagacaaacg tctttcagac ggcattaaag gtgcgcaatg
    18481 gttgggacaa agcattgaat ctttggctga caacgatgcc gtattggtag tcggtgcgaa
    18541 cttgcgcaaa gaacagccgc tcctgaccgc ccgcctgcgc cgcgccgcca aagaccgtat
    18601 ggctttgagc gtattggccg gcagtaaaga agaattgttt atgccgcttc tgtctcaaga
    18661 agccgcacat cccgacgagt gggcagaccg tctgaaaaaa ctgtctgcca atgcggaaca
    18721 cgccgttacc gccagcctga aaaacgctga aaaagcagcg gtgattttgg gcgcggaagt
    18781 gcaaaaccat cctgattacg ccgccattta tgccgccgcg caagagctgg cggacgcgac
    18841 cggcgcagtg ctgggtattt tgccgcaagc cgccaacagt gttggtgcgg atgtcttgaa
    18901 tgtaaactcc ggcgagagcg ttgccgaaat ggcaaacgcg ccgaaacagg cagtcttgtt
    18961 gctcaacgtc gagccggaaa ttgatacggt tgacggtgca aaagccgtag ccgcgttgaa
    19021 acaggcaaaa agcgtgatgg cgtttacgcc gtttgtcagc gaaacgctgc tggacgtgtg
    19081 cgacgtgttg ttgccgattg cgccgtttac cgaaacctca ggcagcttca tcaatatgga
    19141 aggccgtctg caatctttcc acggcgtggt acaaggcttc ggcgattcgc gtccgctgtg
    19201 gaaagtgttg cgcgtattgg gcaacctgtt tgacctgaaa ggttttgaat accacgatac
    19261 cgctgcgatc ttgaaagacg cgctggatgc agaaagcctg ccgtccaaac tggacaaccg
    19321 cagtacatgg acaggggagg gcgttcagac ggcatcagac cgcctcgtgc gcgtgggcgg
    19381 agtgggcatc tatcacaccg atgccatcgt gcgccgttcc gcaccgttgc aggcaaccgt
    19441 ccatgccgcc gtgcctgccg cgcgtgtgaa tccgaacact ttggcgcgct tgggcctgca
    19501 agacggacaa accgctgttg ccaaacaaaa cggcgcaagc gtatcggttg ccgtcaaagc
    19561 cgatgccggt ttgcctgaaa acgtggtgca tctgtcgctg cataccgaaa atgccgcgct
    19621 gggtgcgttg atggacacta ttgaactggc gggagcttga tcatgcaaga atggttccaa
    19681 aacctctttg ccgcaacgct cggtctgggc gatttgggta tcaccgtggg cttggtggta
    19741 tccgtcatcg tcaaaatcgt gattatcctg attccgctga ttctgaccgt cgcctacctg
    19801 acttatttcg aacgtaaagt catcggcttc atgcagcttc gcgtcggccc gaacgtaacc
    19861 ggcccgtggg gcctgatcca gccgtttgcc gatgtgttca aactcttgtt taaagaggta
    19921 acccgtccga agctgtcaaa caaagccctg ttctatatcg gcccgattat gtcgcttgcc
    19981 ccgtctttcg cggcgtgggc agtgattccg ttcaacgaag aatgggtgct gaccaatatc
    20041 aatatcggcc ttttgtacat cctgatgatt acctcgctgt cggtttacgg cgtgatcatc
    20101 gcgggctggg cttccaactc aaaatattcg ttcttgggcg caatgcgtgc ttccgcgcaa
    20161 agcatctcct acgaaatcgc catgagcgcc gcgctggtgt gcgtcgtgat ggtgtcgggc
    20221 agcatgaact tctccgacat cgttgccgca caagccaaag gcatcgcggg cggttccgtg
    20281 ttctcgtgga actggctgcc gctcttcccc atcttcatcg tctatctgat ttccgccgtt
    20341 gccgaaacca accgcgcgcc gtttgacgtg gcagagggtg agtctgaaat cgttgccggc
    20401 caccacgtcg aatactccgg cttcgcattc gcgctgttct tccttgccga atacattttc
    20461 atgattctga tttccgcgct gacatcgttg atgttcctcg gtggttggtt gtctccgttc
    20521 ccacaaagct ggggctttat cggcacgcct tccgcattct ggatgttcgt gaaaatggcg
    20581 gcggtgttgt actggtatct gtggatccgc gcaactttcc cacgctaccg ttacgaccaa
    20641 atcatgcgtt tgggctggaa agtgctgatt ccgatcggct tcgcctacat cgtgattttg
    20701 ggtctgtgga tgatttcacc gctgaatttg tggaaataag tttcagacga cgtatcaagg
    20761 tcgtctgaaa acgcagtttt gaatatttaa cagaatccct gttttgaggg aacataatat
    20821 ggctaactta gtaaaaacct ttctgcttgg cgaattggta aaaggtatgg gcgtaacgct
    20881 caaaaacttt tttgcccgca aagacacgat ttattttccc gaagagaaaa cgccgcaatc
    20941 cgtgcgtttc cgcggtctgc acgcgcaacg ccgttatcca aacggcgagg aacgctgcat
    21001 cgcgtgtaaa ttgtgtgaag ccgtttgccc cgcaatggcg atcaacatcg aatcggaaga
    21061 acgcgaagac ggcacgcgcc gcaccaagcg ttacgacatc gacctgacca agtgcatctt
    21121 ctgcggtttc tgcgaagaag cctgcccgac cgatgcgatt gtggaaaccc atatttttga
    21181 ataccacggc gagaaaaaag gcgacttgca catgaccaag ccgattcttt tggccattgg
    21241 cgacaaatac gaagctgaaa tcgccaaacg caaagccgct gacgcgccgt atcgttaatg
    21301 ctttggggct tcttggaagg ttttaaatat ggaaggactg attaatgcat tgaaatattt
    21361 agccgaacat gagccaatag ataattttga agaaattaga actagaaata gtccgattga
    21421 gttgccaagt ggattaagta attttgaaca aaatattttt ttaaaagaaa atttatcccc
    21481 aaaattacaa aatgatgata gcttgaagac gcattattgg attatccgtg aatggggtgg
    21541 gattaaaagt tttaaacaat ctgctgaaaa tagccagctt attcgtcaat ttttatcgga
    21601 acttaattcg ggaaaattga gtagtggttt gttgaaaatt tcatcattat ctaaattggc
    21661 ttcttttata gattgtgagc gattcgccat ttatgattca cgcgctattt tttcgttgaa
    21721 ttggttgttg tttaaattta caaatgcaga tttgtttttt cagccacaag gtagaaatag
    21781 ggaactagaa atccgaaata tgaacgtatt gtttcatttt tctgatatca aaccgaatta
    21841 tcggaaacca gacgtttcgt ttcatcaata ttgtggattg ttacaagatt tggcgaaaca
    21901 agtttatggt aaacaagcaa aaccgtatca catagaaatg ttgttattca aaattgcgac
    21961 aacgtggatt tgtgcggata tggatcaact gattaagttt gattgtttgc gtaaccagga
    22021 ttttcagact gcttgaaacc atatttttga ttaataaaga aagcatagac tatgactttc
    22081 caactgattt tattttatat ttttgcagtg ataattcttt atggcgcgat caaaaccgtc
    22141 accgctaaaa atcctgttca cgccgctttg catctggtgt tgaccttctg cgtgagcgcg
    22201 atgctttgga tgctgatgca ggctgagttt ttgggcgtga cgctggtggt ggtttacgtc
    22261 ggcgccgtga tggtgttgtt cctgttcgtc gtgatgatgt tgaacatcga cattgaagaa
    22321 atgcgtgccg gtttctggcg gcacgcgcct gttgccggtg tggtcggcac attgttggca
    22381 gttgccctga ttctgattct ggtcaatccg aaaaccgacc ttgccgcatt tgggctgatg
    22441 aaagacattc ccgccgatta caacaatatc cgcgatttgg gcagccgtat ttataccgat
    22501 tatctgctgc cgtttgaatt ggcggcggta ttgctgctgt tgggtatggt ggccgcgatt
    22561 gcgctggttc accgtaaaac ggttaatccg aaacgtatgg atcctgccga ccaagttaaa
    22621 gtacgcgccg accaaggccg tatgcgcttg gtgaaaatgg aagccgtgaa accgcaaacc
    22681 gaatctgccg aagaaagcga agtttcagac gacctcaagc cgaaagagga gggcaaagca
    22741 tgattacctt gacgcattat ctggtattgg gtgcgctcct gttcggtatc agcgcgatgg
    22801 gtatctttat gaaccgcaaa aacgtgctgg tcttgctgat gtccatcgag ctgatgcttt
    22861 tggcagtgaa cttcaacttt atcgcctttt cgcaacattt gggcgatact gccggacaaa
    22921 ttttcgtatt tttcgtactg accgttgctg ctgccgaatc tgccatcggt ttggcgatta
    22981 tggtgctggt gtaccgcaac cgacaaacga ttaacgttgc cgatttggac gagttgaaag
    23041 ggtaaaggta ggttgggtcg agacctgaca agacaccgat gccgtctgaa aacccgatag
    23101 gaaaaacgat gaaatccata gacgaacaaa gcctgcataa tgcccgccgc ctgtttgaaa
    23161 gcggcgacat cgaccgtatc gaagtcggta ccaccgcggg cctgcaacag attcaccgtt
    23221 acctgttcgg cggcttatat gattttgcgg gtcaaatcag ggaagacaac atttccaaag
    23281 gcggttttcg ttttgccaac gccatgtatt taaaagaggc tttggttaaa atcgagcaga
    23341 tgcccgagcg gacttttgaa gaaatcatcg ccaaatatgt tgaaatgaac attgcccatc
    23401 cgtttttgga gggtaatggc agaagtaccc gcatctggct ggatttggtg ctgaagaaaa
    23461 acctgaaaaa agtcgtgaac tggcaaaatg taagcaaaac cctgtatttg caggcgatgg
    23521 aacgcagccc cgtcaacgat ttagaactgc gctttttgtt aaaggacaac ctgaccgacg
    23581 atgtggacaa ccgtgaaatc atctttaaag gtatcgagca gtcgtattat tacgaagggt
    23641 atgaaaaagg ctgaaggtcg tctgaaaagc gatttcagac tgtttcagac gacctgattt
    23701 ggtaggtgat cagacgggag cggatgagaa aagaaattct aggtaagaat aatccggtct
    23761 gaaatattgg aagaagaatg atggataaaa atcagttaga acaagaattt cataaagcca
    23821 tgttaaatat ttatcaggag gctttgaatt tgccgcaacc ttacaaggcg acacgatttt
    23881 tacaaattgt aaatgaattt ggtggtaaag aggcggcgga taaattatta agtacggggg
    23941 aaaagaagac tcagaccggt tttacagagc tgattttgag tggtggcgga gtccacgcct
    24001 tgaaatacag tatggaatat ctggtgttac aaaagccgtg gtgtgattta tttactgaag
    24061 agcaattagc tgtggcacgc aaacgattgg agcgtgttgg atttgtttct ccgaagtaat
    24121 tttgtacgaa acaaacatag atttttaaat caatcggatt caatcaaatg aacgacatga
    24181 ctttatatct catcatcgcc ctcgtgcctt tggcaggctc gctgattgcg ggtttattcg
    24241 gcaacaaaat cggacgtgcc ggcgcgcata cggttacgat actcggtgtg gcggtgtccg
    24301 ccgtgctgtc ggcttatgtg ctgtggggat tcctcaatgg cagccgtgcc aagtttgacg
    24361 agaacgtcta tacctggctg acaatgggcg gtttggattt ctccgtcggc ttcttggtcg
    24421 atacgatgac ggcgatgatg atggtcgtgg taacgggcgt gtcgttgatg gtgcatatct
    24481 acaccatcgg ctatatgcac gatgaaaaag tcggctacca acgcttcttc agctatattt
    24541 ctttgtttac tttcagtatg ttgatgctga ttatgagcaa caacttcatc cagctcttct
    24601 tcggctggga ggctgtgggc ttggtatcgt atctcttgat cggtttctat ttcaaacgcc
    24661 cgagcgcaac atttgccaac ctgaaagcct ttttgatcaa ccgtgtcggt gacttcggct
    24721 ttttgctcgg tatcggcttg gtgcttgcct atttcggcgg cagcctgcgc tatcaagatg
    24781 tattcgctta tctgcccaac gtccaaaatg ccaccatcca gcttttcccc ggtgtggaat
    24841 ggtctttgat taccgtaact tgtttacttc tgtttgtcgg tgcgatgggt aaatcggcac
    24901 aattcccgct gcatgtctgg ctgcccgatt cgatggaagg cccgaccccg atttccgcat
    24961 tgattcacgc cgcaaccatg gttaccgccg gtctgttcat ggtatcgcgt atgtcgccga
    25021 tttatgagat gagtagcact gcgctgtctg tcatcatggt gattggtgcg attaccgccc
    25081 tgtttatggg tttcttgggc gtgattcaaa acgacatcaa acgtgtggtt gcgtattcca
    25141 ccctgtcgca actgggctat atgaccgtgg ctctgggcgc gtctgcctat tccgtggcga
    25201 tgttccatgt gatgacccac gctttcttta aagccttgct gttcttggcg gcgggcagcg
    25261 cgattatcgg tatgcaccac gaccaagata tgcgccacat gggtaacctg aaaaaataca
    25321 tgccgattac ttggctgact atgctgatcg gcaatttgtc gctgatcggt acgccgttct
    25381 tctccggctt ctactccaaa gattcgatta tcgaagcggc gaaatacagc accctgccgg
    25441 gcagcggctt tgcctatttc gccgtcctcg ccagcgtgtt tgttactgcg ttctacgcat
    25501 tccgccaata ctttatggtg ttccacggcg aagagaaatg gcgcagcttg cctgaacatc
    25561 atgacgatca tcatggcgaa gaacatcaca gcttgggcaa aaacgacaat ccgcacgaaa
    25621 gcccgttggt tgttaccctg cctttgattt tgcttgccat tccgtccgtc atcatcggct
    25681 acgttgccat cgagcctatg ctttacggcg atttctttaa agacgtgatt ttcgtcaata
    25741 ccgacgcgca tccgaccatg cacatcatga aggaagagtt ccacggcgca ttggcaatgg
    25801 tgtcgcacag cttgacatcg cctgttttgt atttggcggc tgccggtgtt gccgccgcat
    25861 ggcttttgta cgtcaaactg ccgcacctgc cagcgaaaat tgcacagacg ttccgtccga
    25921 tttacgtttt gtttgaaaac aaatactacc tcgacgccct gtatttcaac gttttcgcca
    25981 aaggcacacg cgcattgggt accttcttct ggaaagtcgg cgataccgcc attatcgaca
    26041 acggcatcgt caacggctct gccaaactgg tcggcgcgat tgccgcgcaa gtgcgtaaag
    26101 ctcaaaccgg ctttatctat acctacgccg ccgctatggt gttcggcgta ttggtactgc
    26161 tcggcatgac cttctggggc ttgttcagat aaaggccggc ttcagacgac ctgttcatgg
    26221 ttgtgtagag gtcgtctgaa aaaagaaact tgagaaacaa tgtcaaacac acaaaccatc
    26281 cgctctgccg acttcaccgc ttcccgcgca tggggtgcac tcgacatcgc caatatgaac
    26341 ggcaccaccg tgcgcctgca ctggacggac cagtcctaca aatggcacgt caacgacggc
    26401 gaggaagtgt ttgccgtgat gaacggcgaa gtcgatatgc actatcgaga aaacggcgaa
    26461 gaacacatcg ttcggttgaa aagcggcgac atcttctacg caggaattgg cacggaacac
    26521 gtcgcccatc cgtgcggcga agcgcggatt ttggtgattg agaaagaagg cagtgcttga
    26581 ggtcgtctga aaagagggtt tcaggctgca atggaattgt aggttgaggt aagaagagat
    26641 gacgcagtta tttttacatt cagatacacc aaatgagaag gaactgattt caatatatga
    26701 gaaagctgtt tcagaggcag tagagctgta tattttctct gcgtatctca ctcattggga
    26761 tgtaaaaaat aagcttaata gcaaattaca agagttcaaa tttatttttg ggcaggattt
    26821 cggattgtct aaaaaagagg ctattcagga agtattgaaa tggcttccgt cccatttgaa
    26881 atttacattg atggtagctc aaggcattca gggttttcat cctaaagctg tattttggaa
    26941 gaatgataag aatgaatatt atgctttaat cggttcttca aatttgactc atgctgcgtt
    27001 taatagcaat tatgaagcaa atattttgac taaaatttct gagcaggatt ttataaaagc
    27061 taaatcttgg gctaatgaaa ttgtgatgaa atctatcccg gtatcggaag attggcttga
    27121 ggagtatcag gaagctgaaa ttaactataa gaaatcaact gttagacaat cagtgatgga
    27181 taagttattt atggagatgc caaattataa tcaagaactg attgcggcac ggagaaagca
    27241 gatgagaaac catcaaactg tgtgtaatca attaaaaaat cttattaagc aatgtgcagc
    27301 aggaaaaata gacaataatg atttttatgg ggaatttaat aaattatggt cttggaaaag
    27361 cgaaaataaa ggagaagggg taggcaatcg ttttcaagat aaaacgtgga aaaggacggg
    27421 taagtcttca gattttagaa aattatgcat tgccatccaa tcagtttttg atgctccact
    27481 tactgagaga gataatgtgg ttgccaagca aattgattgg ctaaaagaat gtggggtttc
    27541 cactcgtggt tcagtttttt ctgaaatgct ttgtcaagaa tatccagata gatatcctgt
    27601 tttaaatgct cccattaaaa aatttttaga agaaaataaa tttaagtctg caaaaggagc
    27661 aagcgaaggc tccaaatata ttgatttaag tatgaagtta agagctttac tagctatgca
    27721 gtcagaaata aaagatttgg ctgaattaga tgttttagta caggcagaat atcgcaatcg
    27781 tacagatatt gattgggaat aagtaaagaa ggggggggga gtattcattc cttctttcag
    27841 acgacttcct aaaacgctgt ctgtaaagac caacccatac aactatattt tttattttaa
    27901 ccacaggtta accactatgt tttccaacta cctactcagc ttggcaatat ggatacccat
    27961 cgttgcgggc gtgctggttt tggcgacggg gccggacagc cgtgcgccgt ttgcccgcgt
    28021 gctcgcttta atcggtgcgc ttgccggttt cttggtaacg ctgcccctgt ttaccggttt
    28081 cgaccgtttg agcggcggct atcagtttac cgagttccac gagtggattc cgcttctgaa
    28141 aatcaactac gcattgggcg tggacggtat ttcagtgctc tttatcatct tgaatgcgtt
    28201 tattacgctg ttggtggtat tggccggttg ggaagtcatt cagaagcgtc cggcgcagta
    28261 tatggcggca ttcctgatga tgtcgggttt gattaacggt gcgtttgccg cgcaggatgc
    28321 gattctgttt tatgtgttct tcgagggtat gctgattccg ctgtacctga ttatcggtgt
    28381 atggggcggc ccgcgccgcg tctatgcgtc ggtcaagctg ttcctctata cgctgatggg
    28441 ttcgctcctg atgctggtgg cgatggttta cctttactat caaacaggca gcttctctat
    28501 tgtcgatttc caaaacatca aacagcttcc gttgggcgtg caacagcttt tgtttgcggc
    28561 gttcttcctg tcatttgccg tgaaagtgcc gatgttcccc gtgcacactt ggctgccgga
    28621 tgcccacgtt gaagcaccga ccggcggttc gatggtgttg gcggcaatta cgctgaaact
    28681 gggtgcgtat ggtttcttgc gctttatcct gccgattatg ccggatgcgg ctcgctattt
    28741 tgcccccgtg atcatcgtat tgagcctgat tgccgtaatt tatatcggta tggtggccct
    28801 ggtgcaaacc gatatgaaaa aactggtggc gtattcgtcc atcagccata tgggttttgt
    28861 aacgcttggg atgtttttgt ttgttgacgg gcagttggac gactgggcat tgaaaggtgc
    28921 aatcattcaa atgatttccc acggtttcgt gtctgccgcg atgtttatgt gtatcggcgt
    28981 gatgtacgac cgcctgcaca cgcgcaatat tgccgattat ggcggcgtgg tcaatgtgat
    29041 gcccaagttt gcggcgttta tgatgctgtt cggtatggcg aacgccggtc tgcctgcgac
    29101 ttccggcttc gtgggcgagt ttatggtgat tatgggcgcg gtcaaagtga atttctgggt
    29161 cggcgcgctg gcggccatga ccctgattta cggtgcatct tataccctgt ggatgtacaa
    29221 acgcgttatt tttggtgcga tccacaatcc gcacgttgcc gaaatgcaag acatcaattg
    29281 ccgcgaattt gcgattttgg cggtgttggc ggttgctgtt ttgggtatgg gcctgtatcc
    29341 gaacgcattt atcgaagtgg tgcatcaggc ggcaaacgat ttgattgccc atgtggcaca
    29401 aagcaagatt tgaggtgtgt aaatgaattg gtctgatttg aatttaatgc ccgcactgcc
    29461 cgaagtcgcg ctgctgtcgc tgctggtgtt attgttgctg gcggacttgt gggtcagtga
    29521 tgacaaacgc cggtggacgc attacggcgc gttggcaacg gtggcggtta cggctgtggt
    29581 gcagcttgcc gtttgggaac agggcagcac gtcttcgttc aacggcatgt atattgcaga
    29641 cggtatgtcg cgtttggcaa aaatggtttt atatgccttg acgtttgccc tgtttgtcta
    29701 tgccagaccc tacaaccaag tgcgcggtat tttcaaaggc gagttttaca ccctgtcgct
    29761 gtttgccctc ttgggcatga gcgtgatggt gagcgcgggg cattttttaa ctgcctatat
    29821 cggtttggaa ctcttgtcgc ttgcccttta cgccctgatt gccctgcgcc gcgattccag
    29881 ctttgccgcc gaagccgcct tgaaatattt tgttttgggc gcgctggcat ccggcctgct
    29941 gctctacggt atttctatgg tttacggcgc aaccggttcg ctggaatttg ccggcgtgct
    30001 cgcctcttcc ttcaatgaag aagcaaacga atggctgttg aaactgggtc tggtgtttat
    30061 cgtcgtcgcc gttgcgttca aactcggcgc ggtgccgttc catatgtggg tgcccgacgt
    30121 gtatcacggc gcgcccactt ctgttaccgc cctggtcggc actgccccga aaatcgccgc
    30181 cgttgttttc gctttccgca tcctcgttac cgggttggga accgtgcatc atgactggtc
    30241 tctgatgttt gccctgcttg ccgccgcctc gctgctggtc ggcaaccttg ccgccatcat
    30301 gcagaccaat atcaaacgta tgctcgccta ttccaccgta tcgcatatgg ggtttatcct
    30361 gctggcgttt atggcgggcg cggtcggctt tgcggcgggt ctctattacg ccattaccta
    30421 cgcgctgatg gcggcggcag ggttcggtgt gttgatggtg ttgtcggacg gggacaacga
    30481 gtgcgaaaac atcagcgatt tggcagggtt gaaccaacac cgcgtatggc ttgccttttt
    30541 gatgctgctg gttatgttct ctatggcggg cattccgccg ctgatgggct tttacgccaa
    30601 attcggcgtg attatggcgc tcttgaaaca aggccatgtt tggttgtccg tatttgccgt
    30661 cgtgatgtcg ctgattggtg cgttctacta cctgcgcgtg gtcaaagtca tgtattttga
    30721 cgaatccggc cgcgcccgtc ccaccgtcgg cggcaacaat gccgcaaaat tccttttgag
    30781 cgtcaacgcg ctgctgttgg tgctgtgggg tatcatgccg caaaccgtta tcgactggtg
    30841 tgccaaagca ttggaaaaca cgctgtaagc cgccgcaacg gcagccgtgt cagaggctgc
    30901 cgtttttgtt aagatatgcc gttccgcaat gcagttcaga cggcatcgct gccgacaacg
    30961 cccaaacaga aagcccacca tgaccgcatc catgtacatc cttttggtct tggcactcat
    31021 ctttgccaac gtcccctttc tcacgaccag actgttcggc gtggccgcgc tcaagcgcaa
    31081 acatttcgga caccacttga tcgagctggc ggcaggtttc gcgctgaccg ccgttcttgc
    31141 ctacatcctc gaatcccgtg tgggagcggt acacaatcag ggttgggagt tctacgccac
    31201 cgtcgtctgc ctgtacctga tttttgcgtt tccctgtttc gtgtggcggt atttttggca
    31261 cacgcgcaac agggaataga caagcataga aatgccgtct gaaacctttt cagacggcat
    31321 ttgtttcatt caagtgcagg ccggcatcgc tgtgccggca cgtttcagcc ggcgatatac
    31381 gccggtttta atatttgcgg gcgactgcaa attctgccaa ctgccgcagg cgcagggctt
    31441 tgtcgccgaa gggttcgagc agcgcgaccg cttcggcaac cagtttgtgt gcgtatgagc
    31501 gcgccgcttc caagcccatc agtctcacat aagtcggctt gtcgttgtct gcgtctttgc
    31561 ccgccgtttt gcccaaaatc gccgtgtccg cttcacaatc caacacatcg tcaatgactt
    31621 ggaacgccag ccccagtttt gccgcataat cgtccaatac cgccagactg tcttcagaca
    31681 gattcgggca ggacatcgcc cccaataaaa ccgccgcacg gattagcgca cccgttttca
    31741 ggctgtgcat ctgttccaaa tcggcttgaa ccatttgttt gccgacattt gccaaatcga
    31801 ttgcctgacc gcccgccatg cccgcgctgc cgcccgcttt cgccagcacc gacaacattg
    31861 ccaactggcg tgcggcgggc agttctgtcg gacggctcaa tacgtcgaag gcttgggttt
    31921 gtaaagcatc gccggtcaga agggcgtttg cttcgccata tttgatgtgg caggtcggtt
    31981 tgccccggcg caggctgtcg ttgtccatcg ccggcatatc gtcgtgaacc agcgaatagg
    32041 cgtgaatcat ctcgattgcc gccattgcct gttctactgc ttcatgcacg gcttcgccta
    32101 attccgaagc cgccagcacc agcatcggac gcagacgctt gccgccgtcc aaagccgcat
    32161 aacgcatcgc ttcgtgcagc gtgtgcggga tttcgttttc agacggcaaa aagcgttcca
    32221 gcagcagctc tgtctgcgcc tgcgccctaa gctgccatgc tttcaaatca ttcgtcggat
    32281 tcaaggttca actccttcgg tccgtccgca tccaaaacct gtaatttctg ttcgacttgt
    32341 gccagcttgg tttgacagta tctgaccagc tcgttgcctt cctgataggc ggcaagcgcg
    32401 tcttccaagg gcatttcgcc ctgcatagac tgcgtcagcg attcaaggcg cgacagtgcc
    32461 tcttcaaacg atttcggggc gtttttcttc atagtttcgg gtgttccggt aaaaagcgga
    32521 ttgtagcatt tttgcaaaac ggatgccgtc tgaaacccga atccggcttc agacggcatt
    32581 gttttgtcct gcaaacggca aggcgttacc cgggcagttc gtcggtgatg ccctgcaaaa
    32641 aggcgaggcg ttcggggctt gccgccctgg tttcggcggc ggctttgaag gcgcagccgg
    32701 gttcggcgcg gtgggtgcag ttgtggaagc ggcattgtcc gacaaggtgg cggaaatcgg
    32761 ggaaatagcg cggcaaatcg gcggcttgga ggtggtgtaa accaaattct tgcaaacccg
    32821 gggagtcgat gagttgggtt tcgccgttca aatcataaag ccgggcgtgg gtggtggtgt
    32881 gttttcccga gtcgagtgcg gcggaaatgt cgccggtgcg ggcggtttgg ctgcccaaaa
    32941 gggcgttggt cagggtggat ttgcccatac cgctctgccc gagcaggatg ttgctgtgcc
    33001 cttgcagggc ggggcgcagg ctgccggcgt tttccagtgc gtgggtttcg atgacgggat
    33061 aacctagcgt ttcgtagaat ttgagttttt cgcgccaaag ggcggtttcg ggcaggtcgg
    33121 ctttgttcag gacgatgacg gcgcggatgc cggcggcttc ggcggcaagc agggcgcgct
    33181 gtagcagccg cacgctcgga ctcgggacgg cggcggttac gatgaggagt tgggtaacgt
    33241 tggcggcaat gagtttggtt ttccacgcgt cttggcggta gagcaggctt tggcgcggta
    33301 aaaaatcttc aatcacaact tgttcggcgt tgacggggct gatgcggacg cggtcaccgc
    33361 aggcgaaatc gacgcgtttt ttgcgggtgc tggcttcgta ggttgtgccg tcgggcgtgc
    33421 ggacgatgta gcggcggccg tagctggcgg taatttgggc ggtgtcgttc atggtttctt
    33481 tggggttggg tgtggaaatg ccgtctgaaa acgggtgttc ggacggcatc ggttcagtcg
    33541 tgctgccact cgacgtgttc gttgaggaag ccgccgctct ggtgcgccca gagtttggcg
    33601 taaagcccgc gtttttcgag gagttcggcg tgtgtgcctt cttcgatgat gcggcctttg
    33661 tcgaggacga cgagcctgtc cattgcggcg atggtggaga ggcggtgggc gatggcgatg
    33721 acggttttgc cgtccatcat tttgtcgagg ctttcctgga tggcggcttc gacttcggaa
    33781 tcgagcgcgc tggtggcttc gtccaaaaga agaatcggcg cgtctttgag catcacgcgg
    33841 gcaatggcga tgcgctggcg ttgcccgccg gagagtttca cgccgcgttc gccgacgtgc
    33901 gcgtcatagc cgcgccgccc tttggcatcg gaaagttcgg ggatgaagcc ggcggcttcg
    33961 gcgcgttcgg cggcggaaac catttcggca tcggtcgcgt cggggcggcc gtaaataatg
    34021 ttgtcgcgca cggaacggtg cagcagcgag gtatcttgcg tgaccaaacc gatttgggcg
    34081 cgtaaagatt cttgggtgat gccgcttatg tcctgcccgt cgatcgaaac cgtgccgctt
    34141 tgcggttcgt agaagcgcaa aagcaggttg acgatggtgg atttgcccgc gccgctgcgt
    34201 ccgatcaagc cgaccttttc gccgggtcgg atggtgaggt tgaagccgtt gagcagcggt
    34261 ttgcccgctt cgtaggagaa atcgacgtgt tcaaatttga ttgcgccttg cggcactttc
    34321 aacggcaggg cgttcggctt gtcgaggatg gtgtgcggtt tggacagggt tgccatgccg
    34381 tcgccgacgg tgccgatgtt ttcaaacagc cgcgcggatt cccacataat gtattgcgac
    34441 aaaccgttga cgcgcaacgc catggcggtg gctgtagcaa ccgcgcccac gccgacctgc
    34501 ccgttgtgcc agagccagat gcccagtgcg gcggtggaga gggtcaggga ggtgttgacg
    34561 atgaagctgc acgaatgcag cagcgtcgcc agccgcattt gggcgcgcac cgtaaccata
    34621 aattcttcca tcgactgctt ggcataggcg gcttcacgcg cgccgtggga gaagagtttg
    34681 acggtggcga tattggaata ggcatcggta atgcggccgg tcatcagcga gcgggcatcc
    34741 gcctgccatg cggcggtttg ccccaatttg ggaatcagca ggcgcatcac cgaagcgaaa
    34801 ccgacaatcc agccgataaa gggcagcagc agccatgagt cgagcgaggc gagaatcacg
    34861 ccggaggtaa tgaaatacac cgacacataa acgaccatat cggcaaccgt catcaccgcg
    34921 tcgcgcaacg ccagcgcggt ctgcatgact ttggcggaca cgcgtccggc aaattcgtcc
    34981 tgataaaaac cgaggctttg gttcagcatc aggcggtgga agttccagcg caggcgcata
    35041 gggaacacgc cctgaagggt ttgcaggcgc acgttggacg cggcaaacgc ccacgcaacc
    35101 gaaaacacca tcatcgccgc cattgccgcc agttcccaac ctttttcggc aaacagttcg
    35161 gtgggcgcgt atttgccgag ccactccacg attttgccca taaattgaaa aaccagggct
    35221 tccataatgc cgatgccggc ggtcagcgca gccagggcgg ctatccattt ccgcacgccg
    35281 gtcatgctgc tccagacaaa ccgccacaag cctttttctg gcgttttcgg ggcggcttcg
    35341 ggataagggt cgattcggga ctcgaaccag gaaaatattt tgttcaacat tgttttcgat
    35401 ttcggtaaaa cagtttcaga cggcatcaaa cacaatgccg tctgaaagga aggacaataa
    35461 cgccatttta cgggaaaagc cgtcgggaag acagcgcggg gcggaaacgc agggtttcgt
    35521 cagggcaaac gccgcgccgc cttcagacgg cattatttca gcaggttttt caaagcaagg
    35581 cgcacgcctt cgcccacgtc cgtcccctcc ggaacgcctt tgaccgccgc ttttgcttcg
    35641 cgttcgctgt aacccagcgc aagcagcgtg ctgacgatgt cttccgtttc gtcggcggcg
    35701 ggtgcggcgg caaacagccc gtccgttacc gtatgcgcga ccagcttgcc gcgcagttcc
    35761 aagaccatac gttcggcggt ttttttgccg attcccggcg cggaagacag gcgtttgaca
    35821 tcttcttctg caaccgcctg cgccagctcg tctgccgtca tcgccgacaa aatgcccaaa
    35881 gcggtttttg ccccgatgcc gctgaccttg atcagttggc ggaatgtttt gcgctcttcc
    35941 gcagtggcaa aaccaaataa aagatgtgcg tcttcccgaa tgataagctg ggtaaacagt
    36001 tgtacgcttt cacccacggg cggcaggttg tagaaggtct gcatcgatac gtcggcctca
    36061 tagccgacac cgttgacatc gatgacgatt tgcggagggt ttttttcaac cagtttgccg
    36121 gtcagtctgc tgatcatgtg tgccgaatcc tgaagtgtcg ggtgcaaaat gccgtctgaa
    36181 accggttcgg gcttcagacg gcacggattg tatcaaattc agtcgtcgcg gcgggaggaa
    36241 atcacgcggc cggtacgggc atcgacaacg actttgtatt cctgtccgtt tttgacgatt
    36301 tcgacatcat agtgcggacg gccgttgtcg tgttcgagat cgatgtcggt gattttgccg
    36361 ccgacacgcg ccaacgctgc tttttcggct tgggcgcggc tgatgatttt gtcttgtttg
    36421 ttgtgttggt gtgcggcgtg tccgtggtcg tcatcgccgt gtccgtcgtg gtgggcgagt
    36481 gcgggggcgg aaatgcccat cagtgcggtt gcggcggcgg tcaagagaag gcgtttgaag
    36541 ttcatgtttt gctcctgcga agtatctggt ggtgtttgaa agacgtaaag gcgggacatc
    36601 aaccggcggt caataccgtc tgaatcatac cgcgcgcctg agcttcggcc tcggcggcgc
    36661 gttcctgcga ggcaaacgga cccattttga cgacgtattc gtaacggcgt ttttcaatgg
    36721 cgaggttcgg gcttgatgtc gaagcggcga agttttgggc ggcttggttc agataggctt
    36781 gtgcttcgtg ttccgtaccg aaagatttca agtcgataaa gatgtctttg ttttcggcaa
    36841 ccggtgtgga ttggcccggg acgatttgtt cgattttgac gtgtgccgtc ccttggccga
    36901 caaagcccaa tttttgcgcg gcggctttgg atacgtcgat gatgcggttg ccgtggaagg
    36961 gaccgcggtc gttgacgcgg acgatgacgc ttttgccgtt tttggtattg gttacgcgca
    37021 catagctggg gatgggcagg gttttgtggg cggcggtaaa ggcgttcata tcgtatcgtt
    37081 ctccgccgga agttttgcgc ccgtgaaacc tgccgccgta ccacgaggcg ttgccggttt
    37141 gcgtgaattc ggcgacttgg tttttcggcg tgtagcgttt tccggcgact ttgtagctgc
    37201 ggttggcgga ggcgtgcagt ttttctgcct tgaccactgc gtcggcggat gccgtctgaa
    37261 gggagtgtgt gccgaatgcg gcggtgagaa ggaaaagggt ttttcgggtt aaagtcaaaa
    37321 cgtgttccgt tcttgagttg aagacgaatg ggcatcatgc ccgccggata cgttccgaac
    37381 cgccgtacag tgcggacggc ggttcggaat gtgtccggat aggttttcag acggcatgaa
    37441 cttgcgttca aacgccgcct gcgtaaccgt gttgccgcca cgcttcaaag agaatcacgg
    37501 caacggtatt ggaaaggttc atactccgac tgccgggctg catgggcagg cggatttttt
    37561 gcgcggcggg caggctgtcg aggatgtcgg caggcagtcc gcgcgtttcc ggcccgaaca
    37621 gtaaaacgtc gcctttttga aacgcggttt catcggggcg cgccgtgcct ttggtggtca
    37681 gggcgaaaat gcgcctgcct gcgagtgcct tgaggcagtc gtcgaagttt tcgtacaccg
    37741 tcaggctggc gaattcgtgg tagtcgagcc cggcgcgttt cattttggcg gaatccaatg
    37801 ggaagccgag cggtttgaca aggtgcaaat ccgcgccggt attggcgcac aggcggatga
    37861 tgttgcccgt gttcggcggg atttccggct ggtataaaac gatggtaaac ataaatatca
    37921 atcacttata ggcgcgtaac cttgccacaa ggcggatggg gtgtcaaaaa atttagttat
    37981 tttttcattg gcgtgcgtgc cagcgtccag cagcagattc ggtttgcgcc cgattttttc
    38041 agcgtctttg ccaattcgtc cagcgtcgcg ccggtggtaa agacatcgtc gattaacaga
    38101 atattacagt tttccggtat cggtgtgcgg atttcaaagg cgtttttgat gtttcgccgc
    38161 cgttcgccgc ctttgagcgt gctttgcggc gggcggtggt gtcggaaaac ggtgtgtcgg
    38221 ggcagtatct gccagccgta gcgttgtgcc agcagcccga cgatgctttc actttggttg
    38281 aacccgcgtt gcagcagccg ctccctgctt agcggtacgg gcaggacgaa atcgaaacat
    38341 tcgtctgtaa gccggtcggg cggattctgc atcatcaggt ctgccagcgg ctgcaccatg
    38401 ctcaaatcag ccaagtgctt cagcgcgtgt atcatattgc tgacgggcgg ttcgtaatgc
    38461 agcgaagccc acatccggtc gaatgcgggc ggttttttct gacagccgcc gcacaccgcg
    38521 ccgccttgga tgtgtctgaa acacaggggg cagctgtttg ccgcgtcggt gcggtatgcc
    38581 gccaaatccg ccgcgcagcc ggcgcagatg ccgtctgaaa cgccggacga accgtggcat
    38641 aatacgcaac gcctgatagt gggcgcgtct gcgatgcgcc gccaacaaga gagaaaatcc
    38701 atgcctgatg ccgtcaaaaa agtttacctg atacacggtt ggggggcgaa ccgccacgtg
    38761 ttcgacgatt tgatgccgcg cctgcctgca acgtggccgg tgtccgccgt cgatttgccc
    38821 ggacacggcg acgcgccctt cgcgcaaccc ttcgatattg aggcagccgc cgacgctgtt
    38881 gccgcacaaa tcgatacgcc cgccgacatt ctcggctggt cgctcggcgg attggtcgcg
    38941 ctgtatctgg cggcgcgcca tcccgacaaa gtccgttcgc tctgcctgac ggcgagtttc
    39001 gcacggctga cggctgccga agattatccc gaagggcttg ccgcgcctgc attgggcaaa
    39061 atggtcggcg cgttccgaac ggattatgcc aaacatatca aacagtttct acaactccag
    39121 cttttgcaca cgcatgatgc ggacagaatc ataggcagaa tcctgcccga tttggcgcgc
    39181 tgcggcacgc cctccgcctt acaggaagct ttggacgcgg cggaaagggc ggatgcgcgg
    39241 catttgttgg acaagataga tgttccggta ctgctggtgt tcggcggcaa agacgcgatt
    39301 acaccgccgc gtatgggtga gtatctgcat cggcatttga aaggaagcag gctggttgtg
    39361 atggaaaagg cggcacacgc gccgtttttg agccatgcgg aagcgtttgc cgcgctgtac
    39421 cgcgactttg tcgaaggggt ttgacatgaa tccgcaggac aaatgttggc aggttcaccg
    39481 ccatcttgcc gaacatgccg accaacggct gacactcgtc cgcaacgcgc ccaaacatat
    39541 cctgcttgcc ggggcggatg cggacatcag ccgcagcctg ctggcgaaac gatatccgca
    39601 ggcggtattt gaagaatacg attcccgtgc ggattttttg gcggctgccg cagccgtgcg
    39661 caagggcggt ttttggcaaa ggtttacggg taagggcgtg gtgcaacact gccaatcccc
    39721 gaccgcgccg ctgcccgaag cgtgtgccga tatgttgtgg tcgaatctcg gactgttggc
    39781 ggcggaacaa atccttcctg tgctgcacaa ctgggcgcgc gcgctggaga cggacgggct
    39841 gctgtttttc acctgcttcg ggcgagatac cttggcggaa ctgaaatgcc gtctgaaaga
    39901 aaatggcatt gaaagccgca gcgcgctttt ccccgatatg cacgacttgg gcgatatgct
    39961 tgctgaaaac gggttttacg accccgttac cgatacggcg aagctggtgt tggattacaa
    40021 aaaggcggaa acgttttggg cggatatgga cacgctgggc gtttggcggg cgatgtcgtg
    40081 gaacgatgaa aacgccgcgc gttcgtgtgt cgggacaata tttgaacggg aaggcggttt
    40141 gagtattacg ctggaaacag tgtacggaca cgccgttaaa aaactgatgc tgccgcaagg
    40201 ggagaacgtg gtgcagtttt ttccgaagag atgatgtgca gatgccgtct gaagctgttt
    40261 ccaagtttca gacggcatct gtctgtgaaa accgatagaa ataaaggaaa tgccgatgta
    40321 tgccgatgcc ggagacgtat attgcgtcta taacttcaga ctgaagcagt acactgcctg
    40381 ccaggttacc cgagttgaag aacacggtgg gaaaaaagca tatgcaaccc tgctggcttt
    40441 ggactggcag ggcaacaaac cgcttggggc ggcggagctg gcggatttga aaccgcttta
    40501 caaagacttt atgtattggg aacgcggtct gcacatgtat aaggcaagtg ccgtcgtgcc
    40561 gacgggatat gtacgggttg ggaataccgc gccgctggtc ggcgaagaca cgcagcagta
    40621 tgcctctttt tggggcgacg gctacgacgt gtaccgtcag ttgagatggc agcagatacc
    40681 cgaaaaacag agaaaggcat tcaaaaaagt cgccaaaagc aaaaagaccg tgatgtttgc
    40741 cggacgggaa tacggcatat ccaaacagaa tttgagcgat gtttgggacg attttgaaga
    40801 cgcgatggaa ttgaaggcgt ttccctgcct gtcttcgctg tttctgacca aatggcataa
    40861 aaatctatat
//
LOCUS       DAWFIT010000007        40121 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFIT010000007 DAWFIT010000000
VERSION     DAWFIT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.007"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(172..2778)
                     /gene="acnD"
                     /locus_tag="WGQ85_000333"
     CDS             complement(172..2778)
                     /gene="acnD"
                     /locus_tag="WGQ85_000333"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0749837.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVVLTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2859..3647)
                     /locus_tag="WGQ85_000334"
     CDS             complement(2859..3647)
                     /locus_tag="WGQ85_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ0749838.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFMLHGSIIFPIAATMAAGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(3799..4953)
                     /gene="prpC"
                     /locus_tag="WGQ85_000335"
     CDS             complement(3799..4953)
                     /gene="prpC"
                     /locus_tag="WGQ85_000335"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0749839.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5039..5914)
                     /gene="prpB"
                     /locus_tag="WGQ85_000336"
     CDS             complement(5039..5914)
                     /gene="prpB"
                     /locus_tag="WGQ85_000336"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0749840.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6540..7850)
                     /locus_tag="WGQ85_000337"
     CDS             complement(6540..7850)
                     /locus_tag="WGQ85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0749841.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(7989..9167)
                     /gene="ftsZ"
                     /locus_tag="WGQ85_000338"
     CDS             complement(7989..9167)
                     /gene="ftsZ"
                     /locus_tag="WGQ85_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0749842.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9286..10530)
                     /gene="ftsA"
                     /locus_tag="WGQ85_000339"
     CDS             complement(9286..10530)
                     /gene="ftsA"
                     /locus_tag="WGQ85_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0749843.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10616..11344)
                     /locus_tag="WGQ85_000340"
     CDS             complement(10616..11344)
                     /locus_tag="WGQ85_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0749844.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            complement(11334..12248)
                     /locus_tag="WGQ85_000341"
     CDS             complement(11334..12248)
                     /locus_tag="WGQ85_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0749845.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKSRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPSIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12361..13770)
                     /locus_tag="WGQ85_000342"
     CDS             complement(12361..13770)
                     /locus_tag="WGQ85_000342"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0749846.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFVDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(13925..14992)
                     /gene="murG"
                     /locus_tag="WGQ85_000343"
     CDS             complement(13925..14992)
                     /gene="murG"
                     /locus_tag="WGQ85_000343"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0749847.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADIAGLPAPDERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRNKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(14996..16321)
                     /locus_tag="WGQ85_000344"
     CDS             complement(14996..16321)
                     /locus_tag="WGQ85_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0749848.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16447..17784)
                     /locus_tag="WGQ85_000345"
     CDS             complement(16447..17784)
                     /locus_tag="WGQ85_000345"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0749849.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKRNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(17868..18140)
                     /locus_tag="WGQ85_000346"
     CDS             complement(17868..18140)
                     /locus_tag="WGQ85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0749850.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     NNLGVMYAERRGVRQDRALAQEWLGKACQNGYQDSCDNDQRLKAGY"
     gene            complement(18255..19337)
                     /locus_tag="WGQ85_000347"
     CDS             complement(18255..19337)
                     /locus_tag="WGQ85_000347"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0749851.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19431..19607)
                     /locus_tag="WGQ85_000348"
     CDS             complement(19431..19607)
                     /locus_tag="WGQ85_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749852.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(19610..20968)
                     /locus_tag="WGQ85_000349"
     CDS             complement(19610..20968)
                     /locus_tag="WGQ85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0749853.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21093..22738)
                     /locus_tag="WGQ85_000350"
                     /pseudo
     CDS             complement(21093..22738)
                     /locus_tag="WGQ85_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(22761..24239)
                     /locus_tag="WGQ85_000351"
     CDS             complement(22761..24239)
                     /locus_tag="WGQ85_000351"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0749854.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24264..26009)
                     /locus_tag="WGQ85_000352"
     CDS             complement(24264..26009)
                     /locus_tag="WGQ85_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0749855.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(26070..26333)
                     /gene="ftsL"
                     /locus_tag="WGQ85_000353"
     CDS             complement(26070..26333)
                     /gene="ftsL"
                     /locus_tag="WGQ85_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0749856.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(26330..27316)
                     /gene="rsmH"
                     /locus_tag="WGQ85_000354"
     CDS             complement(26330..27316)
                     /gene="rsmH"
                     /locus_tag="WGQ85_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0749857.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(27313..27768)
                     /gene="mraZ"
                     /locus_tag="WGQ85_000355"
     CDS             complement(27313..27768)
                     /gene="mraZ"
                     /locus_tag="WGQ85_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0749858.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            28047..29264
                     /locus_tag="WGQ85_000356"
     CDS             28047..29264
                     /locus_tag="WGQ85_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0749859.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            29396..29472
                     /locus_tag="WGQ85_000357"
     tRNA            29396..29472
                     /locus_tag="WGQ85_000357"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29430..29432,aa:Met,seq:cat)
     gene            29480..29555
                     /locus_tag="WGQ85_000358"
     tRNA            29480..29555
                     /locus_tag="WGQ85_000358"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29513..29515,aa:Ala,seq:ggc)
     gene            complement(29846..30667)
                     /locus_tag="WGQ85_000359"
     CDS             complement(29846..30667)
                     /locus_tag="WGQ85_000359"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0749860.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLVGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGIAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(30701..31345)
                     /locus_tag="WGQ85_000360"
     CDS             complement(30701..31345)
                     /locus_tag="WGQ85_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0749861.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQLEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVRAERKLSNHPVQK"
     gene            complement(31348..32193)
                     /gene="ftsN"
                     /locus_tag="WGQ85_000361"
     CDS             complement(31348..32193)
                     /gene="ftsN"
                     /locus_tag="WGQ85_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0749862.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(32299..33795)
                     /locus_tag="WGQ85_000362"
     CDS             complement(32299..33795)
                     /locus_tag="WGQ85_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0749863.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPIKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(33809..34129)
                     /locus_tag="WGQ85_000363"
     CDS             complement(33809..34129)
                     /locus_tag="WGQ85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0749864.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34612..36138
                     /gene="putP"
                     /locus_tag="WGQ85_000364"
     CDS             34612..36138
                     /gene="putP"
                     /locus_tag="WGQ85_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0749865.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     NVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(36228..36395)
                     /locus_tag="WGQ85_000365"
     CDS             complement(36228..36395)
                     /locus_tag="WGQ85_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749866.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            36387..39992
                     /gene="putA"
                     /locus_tag="WGQ85_000366"
     CDS             36387..39992
                     /gene="putA"
                     /locus_tag="WGQ85_000366"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0749867.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDKNISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNDKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         9479 a        11364 c         9976 g         9302 t
ORIGIN      
        1 tttgtccgca cagaaactca tcggataaat aaggtttcct caattccact ttctggattc
       61 ccgcctgcgc gggaatgacg atttacagta gatgcctgcc atatcgagaa ttacgtgaat
      121 gagcaaaatg ttgaacccga cccacgctac gtcttgcttt tcagacgacc tctaagccgc
      181 gttcccttcc aaaaaatcct gtgcaaaccg ttgcaacacg ccgccggctt catataccaa
      241 tacttcttct gcagtatcga ggcggcaggt aacgggaact tcaacggttt cgccgttttt
      301 acggtgaatc acgagggtca ggtcgcagcg cggtgtgcgt tcgccgacca cgtcgtaggt
      361 ttccgtaccg tccagttgca gggtatggcg gttggtgtcg ggtttgaact gcagcggcaa
      421 cacgcccatg ccgataaggt tggtgcggtg gatacgctcg aagccttcgg caacaatcgc
      481 ttctacgccg gcgaggcgta cgccttttgc agcccagtcg cggcttgagc cttgaccata
      541 gtccgcaccg gcaatgatga tgagcggctg tttgcggttc atataggttt cgatggcttc
      601 ccacatgcgc atggtttcgc cttcgggttc gacgcgggcg aacgagcctt ggcgcacgct
      661 gccgtcttcg tttttcacca tttcgttaaa cagtttcgga ttggcgaagg tagcgcgttg
      721 ggcggtcaag tggtcgccgc ggtgggttgc gtaagagttg aagtcttctt caggcaaacc
      781 cattttcgcc aaatactcgc ctgcggcact gacggccaaa atcgcattgg acggcgagag
      841 gtggtcggtg gtgatgttgt cgggcaaaat cgccagcgga cgcatacctc ttaatgtgcg
      901 ttcccctgcc agcgcgcctt cccagtaagg cggacggcgg atgtaggtgg acatcggacg
      961 ccaatcgtac agcggactag gtgctttttg cgctgtgccg gtgtcgaaca tcggtacata
     1021 cacatcgcgg aactgctgcg gtttcacata ttcggcaacg acggcatcga tttcttcatc
     1081 ggcaggccaa atgtctttca ggcggatttc cttgccgtct gcaacgccga gtacgtcgtt
     1141 ttcaatatcg aaacggatac tgcctgccag cgcgtaggca acgaccaacg gaggcgaagc
     1201 gaggaaagcc tgtttcgcat acggatggac acggccgtcg aagttgcggt tgcctgataa
     1261 tacggcggtg gcgtacaaat cgcggtcgat gatttctttc tggattttcg gatccagcgc
     1321 gccactcatg ccgttgcagg tggtgcaggc gaaggcgacg ataccgaagc cgagtttttc
     1381 catttcgggc agcaggcctg cttctttcaa ataaatttcc gccactttcg aaccgggggc
     1441 aaacgaggtt ttgacccacg gtttgcgttt cagcccgaag cggttggcgt tgcgcgccaa
     1501 gagcgcggcg gcaacgacgt tgcgcgggtt ggaagtgttg gtgcaactgg taatcgcggc
     1561 gatgatgacc gcgccgtcgg gcatttgtcc gtctgaaggc tcttcgtaag gcttcgccag
     1621 ccctttcgcc gccaaatcga cggtggcaaa acgggcatgc gggttacttg gacctgccat
     1681 attgcgcgtt acgctgctca aatcaaattt caaaacgcga ggataaacgg cggttttcaa
     1741 ggcatctgcc cacaagcctg cggttttggc gtaggtttcc accaatttca cctgcgcgtc
     1801 gtcgcgtccg gtcagtttca aataatcaat ggtttgctca tcaatagcga acatcgcggc
     1861 agtcgcgccg aactccggcg tcatgttgga aatggtcgcg cggtcgccga tagacaggct
     1921 tctcgcgccc tcgccgaaga attcgacaaa cgccccgacc acgcgttctt tgcgcagaaa
     1981 ctcggtcagt gccaacacaa tatccgtcgc cgtaatgccc gcctgccgtt tgccggtcag
     2041 caccacaccg acaatatcgg gcaggcgcat catggacgcg cgtcccagca ttacggtttc
     2101 cgcttccaat ccgcccacgc ccacggaaat cacgcccagc gcatcgacgt gcggcgtgtg
     2161 cgaatccgtg ccgacgcagg tatcggggaa cgccacgccg tttttgactt ggacgacggg
     2221 cgacattttt tccagattga tttggtgcat gatgccgttg cccgccggaa tcacgtccac
     2281 attttcaaag gcggttttcg tccagttgat gaagtggaaa cggtcttcgt tacgtctgtc
     2341 ttcgatttgg cggtttttgc ggaaggcatc ggggtcgtag ccgccgcatt caacggccaa
     2401 agagtggtcg acgatgagct gggtttgcac caccggattc actttggcag gatcgccgcc
     2461 tttttcggca atcgcatcgc gcagacctgc caaatccacc aacgcggtct gccccagaat
     2521 atcgtggcac accacccgcg ccggatacca aggaaagtcg atttcctgtt ttccctcaat
     2581 cagctgaccc agccagcttt gcagcgtcgg caaatcgact ttgtccgcgc ggttgaccaa
     2641 attctccgcc aaaatgcggc tcgtgtaagg cagcttgtcg taagagccgg gcttgatgtc
     2701 ctcacacgcc gcacgcgcgt cgtagtattc caaatccgta ccgggcagcg gtttgcggta
     2761 acgttggttg gcagccatgt tcagttctcc tgtggatcta tttttcttgt ggtttggggt
     2821 ttcagccgac gtttgtcgaa ggggtcgtct gaaagggttt aaaacatcga aacaatcatc
     2881 tgatacagcg gatttctctc gtctatcaac aatttcacag ccatcgaaat gctgatgaca
     2941 atcagcagcg gcttaatcag cttcgaaccg aagcggacgg caaatctcgc acctaaattc
     3001 gcaccgacaa acgcaccggc cgccatcgtt gccgcaatcg gaaaaataat tgaaccgtgc
     3061 agcatgaata ccgatagcga accaagattg caggcaacgt tcgccaattt ggtgtaagac
     3121 atcgcgttca acagcttgca gccgagcaaa acaataaagg caatcagaaa aaacgagccg
     3181 acacccggtc cgaacacacc gtcgtaaaaa cccaaaagcg gtgcgaccgt cagcccgaac
     3241 agaaaaaaag acattctggc tttgccttcc ttactgccgt cgagcttggg cgaaaacaca
     3301 aaatacagtg cgacaaatat caacaaaacc ggcacgaccg ccagcagaat atctttggaa
     3361 accaagctga ccgataatgc accggccacg ccgcctacaa acgatgctgc ggcaatcggg
     3421 agacctttct tccaatcaat caaacctttg cgtgcaaaag aaaccgtagc tgaaaacgta
     3481 gcagcggctg cttgcagctt gttggtggca attgccgaca cgggaggaat acctgccaac
     3541 aagagtgcgg gcagcgtaat caaaccaccc ccgcccgcaa tcgcatcgat aaatccggca
     3601 atcattgcaa ccaaacccaa agcgagtatt atgtataaat cttccatgtt tcttatcctg
     3661 ttacttgcgc caatacagga ttatcttctc ctattagatt aaacttattt cagacaacct
     3721 ttccaataag gcaaggtcgt ctgaaatcct tagctttgca taccgaaata aagaacaaac
     3781 tgctattgca ttcttcaatt atcgttcttc aatctccaca aacgccaaat cttcagggcc
     3841 tgtgtagttc gcgctcggac ggatgatttt gccgtctttg cgttgctcaa gaacgtgtgc
     3901 gctccaaccg gttgtacggg aaattacgaa cagcggtgtg aacatagcgg tcggtacgcc
     3961 caatttttgg taggaaacgg cagagaacca gtccagattc gggaacattt ttttctcttc
     4021 ccacatcacg ctttccaaac gttcggcaat gtcaaagagg cgcatatcgc cggtttcttt
     4081 gctcaaaccg cgtgccactt ctttaatgac aacgttgcga gggtcggaaa tggtgtacac
     4141 tggatgaccg aaaccgatca cgatttcttt gcggccgatg cgttcgcgga tgtctgcttc
     4201 ggcttcgtcg gcattgcggt agcgtttttg aatatcgtaa gccacttcgt tcgcgccgcc
     4261 gtgtttcgga cctttcaacg cgccgattgc tccggtaatg ctggagtaca tatcagagcc
     4321 tgtaccggca atcacgcggg cggtgaaggt agaagcgttg aactcgtgtt cggcatacag
     4381 aatcagtgaa acgtgcatgg ctttgatgtg ggattcgctt gggcgtttgc cgtgcaacag
     4441 ttgcaggaaa tgaccgccga tggtctcttc gtcgctttca acctcaatgc gtttgccgtt
     4501 gtgcgaatat tgataccagt acaagaggat gctgccgagg ctggcgatca gtttgtcggc
     4561 gatgtcgcgc gcttcacttt ccggatggct ttcacgttca ggatgaacgc agcccagcat
     4621 ggatacgccg gtacgcatta cgtccatcgg atgggtatgt gcaggcaggc tttccaaaac
     4681 tttaatcaca cggataggca ggccgcgcat ggatttgagc ttggttttat aagcggccag
     4741 ctcgaatttg ttgggcagat gaccgtgaat cagcaggtgg gcgacttctt caaactcgca
     4801 tttttgtgcc aaatccaaga tgtcgtaacc gcgatagctc aaatcgttgc cggtacggcc
     4861 aacggtacac aaagcggtat taccggccgc aacgcctgaa agcgcaacgg atttcttagg
     4921 tttgaaggtc ggggtttgag tagtttcagt catggtattt ctcctttgtg tttttatggg
     4981 tttcgggttt tcagacgacc gatgcggatt tgttgaaaga ccgtctgaaa gcggtaaatc
     5041 atttttgaaa caatttatcc agtttttgct cgaaggcatg atagttcaga tgctcgtaca
     5101 gctcggcacg ggtttgcata ctgtccacca ccgccgcctg agtgccatcg cgcataatcg
     5161 cttcgtaaac attcagagcg gctttgcttg ctgcacggaa cgatgacagc ggatacagca
     5221 ccagcgacac gccgttttca gccagctcgc tttgggtata aagcggagtg gaaccaaact
     5281 cggtaatgtt cgccaacacg ggcactttca ccgcatctgc aaattggcgg tacatgttca
     5341 aatcggtcat ggcttcaggg aaaatcatgt ccgcaccggc ttcgacacaa gcttgggcgc
     5401 gttcgatagc ggcatccaaa ccttctaccg ccagcgcatc ggtacgcgcc ataatcacga
     5461 agttctcatc aacgcgcgca tctacggcag ctttgatacg gtcgaccatt tcatctttag
     5521 atacaatggc tttgttcgga cggtggccgc agcgtttttg cgctacctga tcttcgatgt
     5581 gaaccgctgc aacaccggcg cgttcaaagt tgcgaatggt acgggcaata ttgaatgcac
     5641 cgccccaacc cacatcgatg tccaccagca gaggcgtatc cacgttgtcc gtaatgcgtc
     5701 gtgcgtcgat cagcacatct tccattgtgg taatgcccaa atcagggata ccgcaagaac
     5761 aggctgccac gccgccgccg gacagataga tggctttgaa accgctttgg gtggccaatc
     5821 gtgcaaaata agcattgacg caaccggcga cggcaagcgg attcgattct ttcacggctt
     5881 ggcggaaacg tgctccggca gagtgttgac tcatcatatt tctcctttat agactttttt
     5941 tcagtatgga caggcttcca tcacattcgg acggcaaaac acagccatcc gacgcgtcgg
     6001 gcatcccaat ccaatcatta aaaatatatg ggaaaaatta tcttattgat atttaaaacg
     6061 aatcaaagaa aaacagcaga ccgttcggaa ttatgcggca aaaccgcaga caagaagaaa
     6121 acaaggggat tattcagaaa aggggaaaac atctgaattg gtttcatagt aatgttcctt
     6181 tgtagtgtta tgtagtttta tttttcgata atctaaaaca caagactgaa aatgtcaaac
     6241 atttttaagg agactcatta cataatttta atattttatt ttaattccat gattaaacta
     6301 gattaatttt gaattatttg tttcaataaa attgtcagga aatcaagaaa aattttcgat
     6361 aatcaaaatc ctgaaaactt ataaaatatt ttatacataa aaacaatgta ttatttttaa
     6421 gcaatccgat gtattttatt atgtatttga tttttatata atttaataaa aattaaatat
     6481 atcggcaaac aaaatgccgt ctgaaaacct ttcagacggc atttttaata tcgaatcaat
     6541 cagcccaaat accagaattt cagtgcccac aatacggcaa caatccatac cataggcgga
     6601 acgtctttgg tgcggcggca taaaagttta accacggcat agctgatgaa gccgaaggcg
     6661 atgccgtctg caatcgaata ggtaaacggc atgaagacaa tggtcaggaa tgcgggtgcg
     6721 gcttccgtca tatcgtccca atcaatatcc ctcgcactgc ggagcatctg cgtgccgaca
     6781 taaagcaggg cgggcgcggt ggcaaaagcg ggaacacttt tcgccaaagg tgaaaacatc
     6841 aggcaggcga gcatcaatac gccgactgta accgccgtca ggccggtccg tccgcctgcc
     6901 gatacgcccg ccgcgctttc cacataaggc gtggtggaag aagtacccaa agccgcacct
     6961 gccacaatgg cggtagagtc tgcaagcagt gcgcgtttca ggcggggcag cttaccgtcc
     7021 accagcagcc cggcacggtg ggatatgccg accagcgttc cggtactgtc aaatagatcg
     7081 accaagaaga agacgaaaat cacactgacc atgctgacgg taaacaggcc ttcaaaatcc
     7141 atctgcataa aagtcggcgc aatgctcggt acttcgccga tgatgccgtg aaattcattc
     7201 aaacccatca ggctggcaat gacggtaatg gtcaagatgg tgatgatgat tgcgccttga
     7261 acgcggaaat gtcccaatac gaccaccata gcaaaaccga acagtgccaa caacgcggac
     7321 ggctgatgaa tatcgcccaa accgaccaag gttgccggat tggcaacgat aatgcctgcg
     7381 cctttcaggg aaatcagtgc caaaaacaaa ccgataccgg cagcaatcga cattttcaaa
     7441 cccataggca gtgcgttgac cagcatttcc ctgactttaa aaaagctgaa caggataaaa
     7501 atcagaccgg agatgaacac cgcacccaac gcaacctgcc aaggcacgcc cataccctta
     7561 acgacggcaa aggtgaaata ggcattcagc cccatccccg gtgcgagtgc aatcggatag
     7621 ttgccgacaa aacccataac aaaacagccg atggcagacg cgatacaggt agcgacgaat
     7681 accgccccca tatccatgcc ggtctcgccc aaaatcagag ggttgacgat aacgatgtag
     7741 cacatcgtca aaaaagttgt caaacccgcc atcaactcgg tacgcaccgt cgtaccgttt
     7801 gccttcagct taaaaatccc gtccaacagt gtttgttttg aagtgtccat atctgaaagt
     7861 ctgtatcaag tggaaaaatc tgaatccgcc gaccgcaaac cgtacgaaat tgcaacatct
     7921 tgaaaaacat acctcccaaa ccggacaaac cgattcggga ggcagcaggt ttacgggcaa
     7981 acagcacatt atttgtctga attgtgttga cgacgcaaaa tcgcagggat ttcaaagtcg
     8041 tcaagtacgg actgattgtc gaaatccgca gcggtaaggt tcatcgtgcg gataccgcga
     8101 ttggtgcgga tcataccttc gacattgtgg ctttgctcct gtttggacgg agcaaccgct
     8161 tctacctccc ttgccggaac aaaatcgacc gcgccttttt ctttcagacc ggtagcgata
     8221 atggtaatcc ggatggcatc ttcgctcatg gtctcgtctt cagccgcacc gaatttgcat
     8281 tccaaatcgg gatgcgcgct ttggttgacg attttcatga cttcggacaa ctcggacatt
     8341 ttcaagcaac ccggagcagt cgtaatattg accagcacac cgcgcgctcc gtccaaggtt
     8401 acatcgtcca gcagcggact ggaaatggcc tggtcggtcg ccatacgcgc acggtcgata
     8461 ccttgggcat aacccgaacc catcatagcg ataccgcggt tgctcatcac ggttttcacg
     8521 tcggcaaagt cgaggttgat gatttcgctc gggcaagtta ccacttcgga aatgcctgcg
     8581 accgcatcgc gcaatacatt gtcggcggca cggaaggctt cgcgcatcgt tacgtcttca
     8641 cccaatgcag tcatcagttt gtcgttcggg atgatgatca gcgaatcgac gtgttctttc
     8701 aactgttcta accctgcctg tgcgacatgg acgcgcttac cttcatatgc gaacggtcgg
     8761 gtaaccacgg caacggtcag aatgcccaaa gacttggcaa tctcagcaac aaccggcgcg
     8821 gaaccggtac cggtaccgcc gcccataccg gtcgtgataa acagcatatt cgcaccgcga
     8881 atggcttctt caatggcttc ccggtcttcc tgggctgccg cacggccgat atcgggattc
     8941 gcgcccgcgc ccaaaccgcg tgtcagattc gtacccaact ggattctctt cgccgcatgg
     9001 ttttttgcca gagactgcgc atccgtattg gcactgataa actccacacc gcgcacattg
     9061 ttggcaacca tgttattgat tgcattgcaa ccgccgccgc ccaagccgat tactttaatc
     9121 accgcagggc tgactgccga ttctgccacg tcgtaaacaa attccattca aaaactcctg
     9181 ctcgccccat tcagaggacg gtttaaataa attattattc attatataag aagtatcttg
     9241 ctgctggcaa aatacttctc acctgtcaaa cggcaatcca cctgttcaga agctgttttc
     9301 aatccaccgt ttcaatcttg ccaacaaacc gccgccccct tccctctctt gcactgcacc
     9361 gttttccggc tgcggcaagt ttccttccag cttgcatgct gcatgaagca gcccgatggc
     9421 ggtagaaaag cgcggtgtgc ggacgcggtc ggacaaaccg cccatttctt ggggcgcacc
     9481 ggtgcgtaca ggcaaatcga agattttttc ggcaaattcc acaatcccgg tcatcatgga
     9541 cacaccgccg gtcagaacaa tacccgcatt cagcacttct ttggggaaac ccgatttttg
     9601 cagctcgccc agcactacgc caaaaatctc ctgaatacgt gcactgatga ttgctgccaa
     9661 aaccttactg gaaacctgac gcgatgtccg gtcacccacg cccggaactt caatcatctc
     9721 acccaagcct tccgtatcgc atgatgtcac gccataatgg attttaatgt actcggcggc
     9781 atcgagaggt gttctcaacg atttggacaa atctttggta atcagattac caccggccgg
     9841 aatgacggac gtatggcgga tggcgccgtt catataaacg gcaatatcgg tcgttccgcc
     9901 accaatgtcg atgacgcata cgccgaggtc tttttcatct tcagtcagca ccgcctgccc
     9961 gcttgccaac ggctgaagca tgatctgatc gcttttcaag ccgcaccgct cgatacattt
    10021 ttggacattc tgcactgccg tacttgcacc ggtaatgatg tgcacccgcg tatccaaacg
    10081 cacaccgctc ataccgatag gctccctcac gccaagttgg gtgtcgataa tgtagtcttg
    10141 aaccacagca tcgagaattt tttgatcggg cgggatattg attgcctttg ccgtttcaat
    10201 ggcgcggtcg atgtctgcct gcgtgacttc cccgtcttta attttaacca caccttgcga
    10261 attgagactg cggatgtggt tgcctgcgat acctgtggta acgtgggtaa ttttggtatc
    10321 cgccatcagc tcggcatcat tgaccgcctg cctgatggct tggacggtgg catcgatatt
    10381 ggttaccatg cccgcgcgca agccccgtga aggagcctgc cccaaaccga cgatgttgat
    10441 tttgtcgtca tcttgaactt ccccgatcag tgcgaggact ttagacgtac cgatatccag
    10501 tacgctgatg tatctttgct gctgttccat tgttcgtctg ctcttaaaac tgattgaaat
    10561 ttgcgtcgca ccgtttcaga cggcacggcc gtaatctgtc cgatacctgt tcccactatt
    10621 cttcggattc tttttcgggt aaaccgtcgt aagcatagcg gactgaaaat ccgtccttat
    10681 acctcatatc cacataggat aaccgatttt tatttttacg caacagatgc tgccacgctt
    10741 cggtaaaaag ccggaggcgt ttcatctcgt tttcccgtcc gagcctgacg gtgatgccgt
    10801 tgtccaaaac gacaatccac gccgaacgtg ccgtataggt catctctttg atgcccaaac
    10861 cctgttttgc caaaacagtc gaaaattcgt cataacggcg gagcatttcg gcagacgttc
    10921 cttccgcgcc tctgaatacc ggcattccgg gtctgtccaa gcgggcttca aaaacattgc
    10981 cttcgccgtc caccaaggca tggtcgcccc aacgcgcgac cggcttgcgc tcggtcagga
    11041 cgacctcaac cgtgtcggga aaacggcggc gaaccatgac cgacgcaatc cacggatacc
    11101 ggcggtaggc ctcctgtgcg ccattgatgt ccgtcctcaa aatattccca tggatgtatt
    11161 ctttcgccaa actgcccaat gctttcttat cggaataaac caggttgccc ttcagcgaca
    11221 cctgcttgac gggcagatga ttcgaattgt aaaaccaaac cagcccggac gcagcaagca
    11281 gcatcgccat catgacaagc agccagcgcg tcagccgttc catcgcttcg gcattatccc
    11341 acatgtgcgg tcttcaaaat ttcaatacat aaatcggcaa aacccacgcc cgtaacggca
    11401 gcggatttcg gtactaaact atggccggtc ataccgggca gggtgttgat ttccaacaga
    11461 tagagtttgc cgtcggtatc tttgaggaaa tcgacgcgca cgcagccttc cgcaccgatt
    11521 gcctgcgcgc cgcgaaccgc cagttcgcgc atcaggcttt cttcggcttc ggtcagatct
    11581 tccgaaggac attgataaat ggtgtcgttg cggttgtact tggcttcgta gtcgtaaaac
    11641 tcggtcgcgg ggatgatgtg tatgctgggc aggcctttgc cgttcaacac agggcaggaa
    11701 tattcgccgc cgccgataaa ccgttcggca atgatttcgc cctgaaggtg tttcaattct
    11761 tcgtaaacgc ttttcagacg gctttttcct ttgactttta ccacgcctac gctgctgcct
    11821 tcggccgccg gtttcacaaa catcggcagg cccaattttt cttcgacggc atcgaaatca
    11881 gtgtcgtcgt gcaggacggc gaactcggga acgggcaatc ccaatgcctg ccaaatcagt
    11941 ttgcagcggt atttgtccat gccgatggcg gatgcggcga caccgctgcc ggtataggga
    12001 atgcccaaca gttccaatgc accctgaacc gccccgtctt cgccgtaagt accgtgaagg
    12061 atgttgaatg ccgtctgaaa accttgtgcc ttcaattcag acaatggggt ttctttagga
    12121 tcgaaggcgt atgcgtctat gcctttgctt tttaaagcat tcaaaatggc ggtgccgctg
    12181 tccagcgaga tttctcgttc gctggaaaaa ccgcccatca atacggccac tttgccaaaa
    12241 ttctgcattg tttttgttct ttcctgattg ctttatgctt gttaccagag gtcgtctgaa
    12301 acctgatttg cggtttcaga cgaccttgat atgatgttcc gtctgtcagg cgggtgtgcc
    12361 tcaaatctgt ttcgacaatt ccagcagcgc ggcggggacg cggttgatgc ttcccgcgcc
    12421 catattcaac acgatgtcgc cgtcctgcaa aacgttcaac agcatttcgg gcagatcggc
    12481 aacgttttcg cagtaaatcg gctcgagttt gcccaacacg cggatggcgc gggcaagagc
    12541 gcgggaatcg gcggcggcaa tcggctcttc accggcggca tacacttcgg tcagcaccag
    12601 cgcgtcaacg gtattgagga ctttggtaaa gtcttcaaac aaatcgcgcg tgcgggtata
    12661 gcggtgcggc tggaaggcga gtaccaaacg tttttccgga tacgcgccgc gtgcggcgga
    12721 aagggtcgcc gccatttcga cggggtggtg tccgtagtcg tccaccaaga gcgcggtccc
    12781 gccgtttggc aacttgatgt cgccgtattt ttggaagcgg cggccgacgc cttcaaagcc
    12841 gagcaagcct ttttggatgg cttcaaccga tgcgccgact tccaacgcaa caccgatggc
    12901 tgccaaggca ttgagaacat tgtggcggcc gggcatattc agcacgactt caaacgaccc
    12961 ctgctcatgt cctttcattt gaacatggac ggtgaatttc atttgcgcgc cgacgttttc
    13021 gatgtcggtg gcgtagatgt cggcggtatc gtccaagccg taagtggcat aaggtttgct
    13081 cactttgggc agaatcgcgc ggacgtgttc gctgtcgata cacaaaaacg ctttgccgta
    13141 gaacggcata cggtggataa aatcaacaaa cgcctgatgc agtttttcaa cgctgtgtcc
    13201 gtaggtatcc atgtggtctt cgtcgatatt ggtgacgaca gacataatcg gggttagatg
    13261 taggaaagag gcatcggatt cgtcggcttc ggcgacaatg tattcgcctt tgcccaaacg
    13321 ggcgttggta cctgcggcgt tgagtttgcc gccgataacg aaagtcgggt caagtcctgc
    13381 cgcgccgagg atggaggcgg tcaggctggt ggtcgtggtt ttgccgtgcg tgccggcaat
    13441 ggcgatgccg tcacggaagc gcatcaactc cgccaacatc agggcgcgcg gaataacggg
    13501 aatttgctgc tccaacgcag cgacaacttc gggattttct tttttgacgg cggtagaggt
    13561 aacgacgaca tccgcaccgt taacgtgttc tgcggtatgg ccgggataaa cttgaatgcc
    13621 caggctgccc aaatgctcgg tagcggcatt tcgcgcctga tccgaaccgg aaactttaaa
    13681 acccaaattg tgcaagactt cggcgatgcc gctcatgccg acgccgccga taccgacaaa
    13741 atggatgttg gtcactcgat ttttcatcat aatgttgcgt tccggtggat tttcgatgcg
    13801 taaaggcgtt attttaaagg gctgaccgtt tgcgcgccat agttttctga caaatatata
    13861 gcggattgaa ataaaaaaca tccatgccgt ctgaacggct tttcagacgg catggttcgg
    13921 cagtttacgc cgcacacgca atcgcggctt cggcaacgtc gtccgcgctg tgcggcaatg
    13981 ccaaagtacg ggcgttttct gcccatttga gacatttttc gcggttcagg ctgccgagga
    14041 tttcggcgag tttttccgcc gtcaactggg tttgcggcaa cagcaatccc gcctccgcct
    14101 gcaccataaa acgcgcgttg gcggtttgat ggtcatcaac ggcgtgagga tacggcacta
    14161 acaacgcacc caatcccgcc gccgtcaact cggcaatcgt cagcgcgccg gcacggcaaa
    14221 tcaccaaatc ggcatcgcgg taggcggaca ccatatcggt aataaattcc acgcattcgg
    14281 ctttcacgcc cagcgcgtcg taatccgcct gcaagctgcc cagcttgttg cgccccgatt
    14341 ggtggtacat ctgcggacgc gcattgtcgg gcagcaaagc caatgcctgc ggtacggttt
    14401 tgttcaaaac gtctgcaccc aaactgccgc cgaccaccaa aattttcaga cggccttcgc
    14461 gcccttggaa gcgttcgtca ggcgcgggca gcccggcaat atcggcgcgg acggggttgc
    14521 cgaccaagcc gccttcgtgg ctgaacgctt tcggaaaagc gtacaacacc cgcttcgccc
    14581 agcgcgacag gtggcggttg gacaaacctg ccacggcgtt ttgctcgtga atcacaatcg
    14641 gcacgcccaa gagtttcgcc gccaaaccgc cggggaaggt aacgaagccg ccgaagccga
    14701 tgacgcactc gacacggtgt ttgcggataa tccgctgcgc ttcgcggacg gtttgataca
    14761 aagtaaacgg cagcatcagc ttgcgtttga tgccgttgcc gcgcacgcct ttaatcgcca
    14821 gcgtttccaa gcgtatgccg tattgcggca cgatacgctc ttccatcgaa tccttgctgc
    14881 ccagccaaat cacatgatgg ccgcgcgcgc gcaatgaatc cgccaccgcc agcgcgggga
    14941 aaatatgtcc gcccgttccg cccgccatca gcataaaggt tttaccgccc atgatttact
    15001 ccacccgata accgcgcatt ttccggcggt tttcataatc tatacgcaac agcagcatca
    15061 tgctgatcag catgaaaaag actgacgaac cgccatagga catcaacggc agcgtcagac
    15121 ctttggtcgg caaagcaccg atgttcacac cgatattgaa gaaactttgg ataccgatcc
    15181 aaatgccgat acccgaagcg atataggcgt tgaaagtcaa acccaaatcg cgcgactgct
    15241 tgccgatgga aaacgcccgc accaccagcc agccgtaaca gaatatcagc acgcacatac
    15301 cgaagaaacc gaattcttcg gcgatgatgg caaaaataaa atcggtatgc gcttccggca
    15361 gaaagccgcg tttgctcaaa ctcgcaccca aacccatacc gaaccactct ccgcgcccga
    15421 ttgccatcag agagtgggta agctggtagc cggcaccctg cgggtctttc cacgggtcca
    15481 aaaatgccac tacccgctgc acacggtagg gagcggcggt aatcatcagc accatcccgc
    15541 ccaagacgct gcctaccagg acgaaaaaat atttccacgg caatcctgcc aaaaacagca
    15601 ttccaacggc aatgacggta atgacgacaa acgaaccgaa atccggctgt accattatca
    15661 gcaccaaacc gaacgccacc agcataatcg gcaggatgat cgcccggaaa cggccgtaca
    15721 tttctaatgt ttcacgacgt gcctgcggat tggtggcgga catgatcaga ttggccgtcc
    15781 cccgccaaat cgactgccaa cccaaacttt ccatgctgcg caacacttct tcacggcgcg
    15841 tgaacaggct tgccaaataa aggatgaccg ccagcttgaa cagctcggtc ggctggaaat
    15901 tcaacggacc caaaggtatc caacgggtcg cgccattgat ttcgcgcccg gcaatcaata
    15961 cgactaccag caacaggccg gatagggcaa aaatccacgg cacaagccgc cgccatgtcc
    16021 tcatcctgca aagaaaccat aaaaaaccgc tcgctatcaa gccggcaacg acgaaccccg
    16081 cctgtctggt caaatagaaa aactgatcgc cgccttcttt tgatgccaaa tacacagaag
    16141 ccgaataaat catcagcagg ctgaacgccg tcatcagcac caccatccac aaaagcggcg
    16201 cgtcgaattt cctgccgtcg cgcacaatcg gcctgtcgag cagcagagtg tggacaccgt
    16261 cgcccacttt taccaatact tccgaaatct tcaaaaaaac cacctgccag tctgtttgca
    16321 cctgccgcaa agggcaaaaa tttcagacgg cagacaatgc cgtccgaaca tacgatacat
    16381 cccaaatcgg tattctaaat ctttacttgc cgcccaacaa tgacggcgtt tgcgtttcag
    16441 acggtatcac aaagccttaa acgccccgat aaacacttcc gaacggtgcg cgtagccttt
    16501 aaacatatcg aaactcgcgc aggcggggct gagcaacaca atatcgcctg cttcggcttg
    16561 ggcatatgcc gtctgaacgg cttctcccaa agtggcgcag tcggtcatat tcaagccgca
    16621 gccgtccaaa tcgcggcgga tttgcggcgc atcgacacca atcaagaaca cgccttttgc
    16681 cttgcctacc agtgcatcgc gcaggggcgt gaagtcctgc cctttgccca tgccgcctaa
    16741 aatcacgaag agcggatttt gcaaaccggc aatcgcagcg gcggtcgcac cgacgttcgt
    16801 acctttgctg tcatcgatga acaccacgcc gtttttctcg ccgatctttt ccacgcggtg
    16861 cggcaggcct tggaaggttt taacgtgttc gagcaatgct tcgcgcggca aaccgacggc
    16921 ttcgcacaag gcgacggcag ccatcacgtt ggcggcgttg tgcaagcctt gcaacggaat
    16981 gtcttgcgtg gcaatcaaat cttcattgcc ttgtttcagg cggcctgtct cgcgttccaa
    17041 ccaaaaatcg gcttcgtgtt ccaacgaaaa ccattttacc tcgcgcccgg cgcgcttcat
    17101 cgcacgacaa aacgcatcgt ccgcattcaa aacctgcaca ccgtcgccac ggaaaatctt
    17161 atccttagta tgcgcgtagt cgagcaagtc gtcgtagcgg tcgagatggt cttcggaaat
    17221 gttcagcacc gtcgccgcag tcgggcgcag gctttcggtg ttttccagtt ggaagctgga
    17281 aagctccaac acccacacgt ccgccttttt gccttcgcgc tgcaattctg cctccaaaac
    17341 cggcgtgccg atattgcccg cgataacggt atccagcccg cacttgatac agagatagcc
    17401 gaccaggctc gttaccgtcg ttttgccgtt gctgccggta atcgcaatca ccttgtcgcc
    17461 gcgacggttc acaatgtccg ccagtaattc gatgtcgccc aacacgcgtc cgccgtttcg
    17521 tttaaaggcc tcgatatccg gctgccgctc gctgatgccg ggactgagcg ccaaaatatc
    17581 gaaaccgttg tccagcgcat ctttcagacg gcccgtgtaa aacaccagcc cgtcaaacat
    17641 cttgccgatt tgcgacacgc gctcggcttt cagctccgca tcataagcgg caacctccgc
    17701 gccgtttttg cgcagatagg caatcatgga aatacccgta ccgccgagtc cggcgacgag
    17761 gatttttttg ttttggaaag tcattttggt ttgtcctaaa acaaatcata ttgagcagga
    17821 gatgtccgcc cctgcccaag ccgctttcag acggcatcgc gagctgttca ataacccgct
    17881 ttcaggcgtt ggtcattgtc gcagctgtct tggtatccgt tttgacaagc cttgccaagc
    17941 cattcttgtg caagggcgcg gtcttggcgc acgccgcgtc tttcggcata catcacgccc
    18001 aaattgtttt gggctgctgc atttccctgt gctgccgcct gcaagttttc ccgaaaatcc
    18061 gatacgtcat ccgcccacac cgcttggttc aagcccaagg caatcaaggc ggcggcaagc
    18121 catttgactg tctgtttcat ggttttactt ctgttttagt ataagccgcc gttaacgata
    18181 gggctgggcg gattgtcgcc gcaggtttat tgcgcgttca aatgccgtct gaaaggtgtt
    18241 cagacggcat aggttcagcg gattttgagg gtactcaaac cgatcaacac caagacgatg
    18301 gtaataatcc aaaagcggac gacgacttgg gtttctttcc agcctttttg ttcgtagtgg
    18361 tgatggatgg gcgccatcag gaagatgcgt tttttggttt tcttatacca gccgacctga
    18421 agcataacgg atacggcttc tacgacaaat aatccgccca taatgacgag gacaaactct
    18481 tggcggacga tgacggcgac ggtaccgagc gcggcaccca atgccaatgc accgacatcg
    18541 cccataaaga cttgcgcggg ataggcgtta aaccacaaga aaccgaggca cgcgccgcac
    18601 atggcggtac agaaaatcac cacttcgttt gcgccggcaa cgtaaggtaa ttgcaggtat
    18661 tgggcaaatt gtgagtggcc gctggcatag gcgaagatgg cgaggccggc ggcaacgagg
    18721 acgacgggga aggtcgcaag gccgtccaag ccgtcggtga ggttgacggc attggatgtg
    18781 ccgacgatgg tcaggtaaga caacaccaaa aagccgacca cgcccagcgg cagggcgatt
    18841 tgtttgaaga acgggacaat caaaatattg ttggcggaat tggcggcaag gtaaaacaat
    18901 gccaaaccgg cgataatggc aacgcttgac tgccacacca ttttgaattt ggcggacacg
    18961 ccgttggggt ctttatagac gactttgcgc cagtcgtcgt aaaaaccgag tgcgcccgtg
    19021 gcgagcaata cgcccaagag aatccagata tacgggtttg cccagttgcc ccacaacagg
    19081 gtggacacgg taatggcggt cagaatcagc gaaccgccca tcgtcggcgt gccgtttttg
    19141 acgaggtggg tttgcggacc gtcggtacgc actgcctgcc cgcatttgag cgcggtcagc
    19201 ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcca aggcggtcaa cgccgccatg
    19261 acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt gctgaaatgt
    19321 gcgagccata aaaacatggg gcttcctttt tttgtttttg tcgttacatg ggcttgtgcc
    19381 cgtttgaggt tggcttgccg cccggcaagg tttcggacga cttttgcaga ttaacgtgca
    19441 gagactttcc caatcacgca aatcgggtaa ctccccggat ttttgccgcc acccaagtct
    19501 aaacataaat cttcataaag atacgcctgc gtctgcattc cggcataaaa cgcgccgacc
    19561 agcgcggcaa tcaacaataa ggctttaaaa aaacgtcgac ttttcatttt cacttatcct
    19621 ccaatgcctc gaccacttct tccatctgca taaagcgcga acctttcacc aacacggtgg
    19681 cgcgttcggg caaatcgtgg cgcaacactt gaatcaacgg gtctttggcg gcgaaccaca
    19741 aaccgtccgc gccaaatttt tccgccgctt cgacgctgtt gtcgccgaca aaataagccg
    19801 cttcgatgcc ttggtcgcgg gcgtacgcgc cgacttcggc gtgcatggcg gcggcttcgt
    19861 cctcacccag ttcgcccata tcgcccatca cgaaaatacg cggcgcaggc atacgcgcca
    19921 acacgtcaac cgcagctttc atgctgtcag ggttcgcatt ataagtatcg tcaatcaggg
    19981 ttgcgccctt gattccggat ttgacgttca gacggccttt gatattgctg aagcctttca
    20041 aaccttccgc cacatcgttc aaactcaaac ctgcagccaa agacagcgcg gcggcggcgg
    20101 cggcgttgtg gacattgtgg cggccgggaa cgggcagaac cacggctgcg cactcgttgc
    20161 cgcacaccaa atcaaattcg cacgacaacg gtttcagcac gatattttcc gcgtggacat
    20221 cgccgctatc gatgccgaaa gtgcgcgtat tcaaattaag cgttgccgtt ttgaagacag
    20281 ccatattggc atcttcttga ggaatcagcg ccatgccgtc tgaacataag ccttgataaa
    20341 tctcgctttt cgctttggca atatcgccca ctccgtcgaa accgcagccg acatgggcgc
    20401 gcatggcgtt gttgaccaat gcggcatcgg gtttggcgat ttgtgtcaaa accgccagtt
    20461 cgccaaaatg gttcataccc atttcaatca cggcatagcg gtgtttttcg tttaatttca
    20521 acaaagtcaa cggcaatccg atgtggttgt tgaagttgcc tgccgtcgcc aaaacggcat
    20581 tatcgccgaa acggcggcgc aataccgcag ccaacatttc cttcaccgtc gtcttgccgc
    20641 ccgagccggt aataccgaac acaaacgggt tcacattctc gcgccacgcc ttcgccaaca
    20701 tttgcaacgc ggtaagcgtg tcatcgactt tcaacgcgcc atccattgca acgcaatctt
    20761 cgcgcgaaac cacaaccgcc gccgcacccg cagccaatac gtcttcaaca aaatcatgcg
    20821 catcaaaccg cccgcccgcc aatgcgaaaa acacatcgcc cgcgcggatg tcgcggctgt
    20881 cggttacgat gcgcgacacg ggtttgcttt cagacggcat cggaagcttg agggcttggc
    20941 agatgaaatt taggtccagt ggtttcatat ttactttcgt taatattcgg gcggcggaca
    21001 catcggtagc ggctgatttt tttatcgcct gttttgctgt ggtaaaacac agattatttt
    21061 cccattctca ttcggcattt tttctgtacg tatcattttt tagacgtatt tttagtcgat
    21121 ttgccttttc ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat aaaggcaagg
    21181 ttattgcctt cgccctgcac atcgggaaca ttcccccaaa aatcatagcc gtcatcgggc
    21241 aactcgtcgg tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt aaacgcgctg
    21301 cgcctcactt taatcatttc gcgccgcgta tcatcggacg aaattttaac cagcggcacg
    21361 ccgtagcttt gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc tttaccgaca
    21421 tgcatcaaac cgtggtcgga aaagtaaacc aaagaccagc tttctttatt ttcattcaat
    21481 atcttaactg tatcttctaa aaatttatcg gtttgcgcga tggtggaaac atagcaggat
    21541 attttttcag tttgatactg aaaccgccgc gcatccttat ccaaacgtgt gcaaaaatca
    21601 ctgtgcgaac ccatcaggtg catcacaatc agccgaggct tcgtgcctgc atttcctatc
    21661 aaaacccgtt tgaacgccgg caacaaaagg cggtcgctca accccgcgct tttgccataa
    21721 tcaccccttt gggtaaacca cggataatcg ctgcgtaggg cataggtgga aatttcgttg
    21781 gcaaaatgcc ccaacattcc ttgattagac agccacgccg tccgaaaacc cgcctgcttc
    21841 gccaagctga cgatgttatt gttcggttct cccggcagcc ccaaagtctg cggcagcgaa
    21901 agattcgtcg cgtgggcggt cgattggtaa ccgtttatca acagcccttt ggtctgactc
    21961 aaaaaaggcg tatcgggcaa tgggaaaccg taaacattca tataatccga acgcgcgctc
    22021 tcaccgataa ccacaacata atttttatac ttgggcgcaa catgacggat atgccatgtt
    22081 gacgcttttt ttgctgcttc caaaatgtgg gcgcgcttgg cggcatattg tgccggcgcg
    22141 gaagccaaat cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc aggttctcgc
    22201 aaatctttat cgctggcgat tttatccatc accgcgcagg acaaaatcaa agtcaataat
    22261 atagtcagcc atattttgct gcggcgttta tagtttttta cgtcagcaaa tacccccccg
    22321 gtacacaata tctccaaact gtcaagccaa aaatgaaaaa taatgcctgc acaaaataaa
    22381 gcgacccggg aagattgccg acaaattcac gcgcctcggc aggattgctt tccaatatcg
    22441 aaccgactat ctgataagac ggcgcaccat acagccagcc gaccggcaaa tatagggcgg
    22501 ttgtgccgac gtaaatcagt aaaacaactg aagaaacgcg cgggaaaccg cgtgccaata
    22561 ataataaaaa tacaaccgaa gccaaagcac ccacggcaga ataacggtag ccgtattcat
    22621 attccaagtg ataacctgat gcgatggccg cgccaaaaca aaaggctaaa agcgcgcaaa
    22681 tgcccaatgt attttttaat gtctgatcca tattttttat tcgggcggat tgtttcatac
    22741 gatgccgtcc gaatatccca ttatttacga gttaacaaag cctgtccgac gatttcaaga
    22801 tcggaaaaac ggtgcttcac gccttgtaca tcctgatagt tttcatgccc tttgccggca
    22861 atcaggatga tgtcgtttgc ggcggcttgt tcaaccgcat aacggatggc ggcggcacgg
    22921 tcggcttcga cgcattcggg cgcgggaacg gcaggcagga tgtcgttgat gatgtcgtgc
    22981 ggattttcca aacgcgggtt gtcgctggtg acgacgactt tatccgcgcc ctgtacggct
    23041 gccgcgccca tcagcgggcg tttgccgcga tcgcggttac cgccgcaacc gaatacgcac
    23101 cacaaagccg caccctgcgg tttgatttcc tgcaaggtgg cgagtgcttt ttccaatgcg
    23161 tcgggcgtat gggcataatc gacaacgacc aaaggtttgc cgctgttcat gatgcagtcc
    23221 atgcgccctg aagcgggacg gatttttgcc agcacatcca atactttatc aagcggatag
    23281 ccgttggcgc acagcaaggc gatgcaggcg gcgaggtttt gcgcgttgaa ccgtccgagc
    23341 aggcgcgtgc ggcatttccc ttcgccccac ggggtttgga atacggctgc tatgccgtct
    23401 gaagaggcgg tgaaatcgat aatgcggatg tcggcgtgtt cgctgaagcc gtagctataa
    23461 acggccaaat cgggacagtc ttttttcaga cgacctacga gttccgcgcc gtattcgtca
    23521 tccacgttga tgactgcgtg tttcaagccg tgccagtaaa acaggcgcga cttgatggca
    23581 ccgtaggctt ccatcgtgcc gtggtagtcg aggtggtcgc gggtgaggtt ggtaaagatt
    23641 gcgctgcgga atgacacgcc gttgacgcgc gactggtcaa gcccgtggct ggagacttcc
    23701 atcgcggcga ctgttgcgcc ttgttgacgg aaacggtaga gcagggtttg gacatcgacg
    23761 ggggcgggtg tggtatgcgt ggtttcttcc aatgcacccc aaaagccgtt gccgaccgtg
    23821 ccgacaatgg cggttttttc gcccaacaaa tcggcagctt gcgccagcca ttgcgtgatg
    23881 gaggttttgc cgttggttcc ggttacgccc cagaatttga gaccgtctga aacgttgccg
    23941 taaacttgcg ccgccaatat gccggcacgg tgtttcaaat ctttgatgcc ttgattgggg
    24001 actttccatt cgggattcca cgcaaatttg ccgtcgtcgt cccaaaaaac aaaagccgcg
    24061 ccgttggcaa cggcggcggg gatataactg cgtccgtcgg catattcgcc cggacaggca
    24121 acgaaaatat cgccttgttt gatttggcgg ctgtctgaat gcaacaaacg ccctgccgcg
    24181 tttgcacacg acagggtcgg gatgccggtt tcagccaaag gggttaactt gctgaacata
    24241 aaacaatctc gttgatactc ggattaagac ggtgttttga cggctgcggc ggtcaggggc
    24301 ttggtcgggg aaatgcccaa gatgttcagg ctgccgccca taattttttt gaagggcggc
    24361 cctgccacta cgccgccgta ataaccgtgg gcagtcggtt cgtcaatggt taccgccaca
    24421 atcacacggg ggtttttggc gggggcaaaa ccgataaagg tagcgatgtg tttgttgtcg
    24481 gcataacgcc cgttgacgaa cttgcgcgcc gtgccggttt tcgcgccgac atcgaaaccg
    24541 tccaccgcac ccgccgtacc ggtgccgccc ggctcggtta cggaaaccat cagattgcgt
    24601 acctcgcgcg cggtcgattc tttgaatatg cgtttgcctt gcggcgcaac cgcctgtttt
    24661 tcaaagctga ccggcagcaa aacgccgtcg tgcgtcagtg cggtataggc gcgcgccaat
    24721 tgcagcaggc tcaattgcag gccgtaaccg aaagacatcg tcgcctgttc aatgggccgc
    24781 cacctgcgcc aatttctcaa caaacctgca gtttcgcccg gaaagcccga gtgcatacgc
    24841 acaccgatgc ccaactcatg atagaagtca tacatttctt cggcaccgaa acgcgcagac
    24901 agtttgcttg tgccgacgtt ggacgatttc tgcatgatgc cgcgcacatc caaagagggg
    24961 taaacatggg tatcgcgcac gggagacggt ccgattttat aaggctgcgt attcagccgt
    25021 tcgttcaaat cggttttgcc cgcatccaat gccttcgcaa tcacaaacgg tttgattgcc
    25081 gaaccgggtt cgatcatatc ggttacggca cggttgcgcc gctgttcgct gtctgcccgg
    25141 ccgggcctgt tgggatcgta ggcgggcgta ttggccaagg cgaggatttc ccccgtgcgg
    25201 gcatccaaaa ccaccaccgt tccggctttt gcctgatggt attcgaccgc cttgttcaac
    25261 tcttcatagg ccaaggtctg aatcctctga tcgagggaaa ggatgatgtc tttgccgttt
    25321 ttcggggctt tattgcgcgg ggagtccaag ctgtccacaa tattgccctg ccggtcccgc
    25381 aaaacgactt ccgcgccgtc ttcgccatgc aggctgtctt caagcgaaag ttccaaacct
    25441 tcctgacctt tgccgtcaat atcggtaaat ccgatgacgt gtgcaaacag gttgcccatc
    25501 gggtaatggc gttttaattc tttttcaaat acaaagtttt ccaaacccaa ggctttgacc
    25561 tcttcggcaa ccttgggatc gagctgccgc ttaatccaga taaacgactt gcctttctgt
    25621 tcgagcttgt tcctcaaaac atcaaccggc acatcgacaa gctcggacag gcgttccaat
    25681 tgtgcggcag acggcatttc cttcatctct ttaggcacgg caaacaggga ctccgtcggc
    25741 gcactcaacg ccaaaaccgc accgttccgg tccgaaaccg taccgcgtgt agccggcaat
    25801 gtttgagtcc gcacaatccg gttgtcgccc tgttctttca aaaagttata cgttaccgtc
    25861 tgcagataca gtccgcgggc aatcagaccg gcaaacaaga ccgctattgc catcaggacg
    25921 aagctaatcc gtccgttact ggtcatcggc tttttgacct gctcttcttt gggcagcatc
    25981 cgaggcttat attcgctctt aatcaacatt tttacttctc gttattatta tcctgacgca
    26041 ggaatccgat tccggcacac aggctgcttc tatctttgat gctccaccat aaaggtattg
    26101 cccgaaaccg gcggatggag gttttgtttt tctgccgccg ccctgatcgc ttcgtggttc
    26161 gccaaacgcg cctgttgcag cctcatttgc gcataatcct gctccaaggc gatttcctgt
    26221 tttttcgcct tgtccaaagc tgtgaagttg agcctgtact ggttttgctg catcacaacg
    26281 gaaaaagcgg aaacgcacac cgcaagcagc agaaggaaat tcaatttgtt cattgccatt
    26341 cagacgtttt tctctgtgat tgttccggta tcggaccggc agtccgctcc gccacacgca
    26401 aaaccgcact tctcgccctc ggattggcgg caatttccgc ctcacccggc tttaatgccc
    26461 tgcccacgat tttcaggggc ggctcgggca aatccgcttc cctgaccgcc gcccagcgcg
    26521 gcaggggcgc gtgttgcgaa tattttttga caaactgctt cacaatgcgg tcttccaacg
    26581 aatggaaagc aatgaccgcc aaacgtccgc cctctttcag acgacacatg acctgcggca
    26641 atactgcccc tacttcttca agctcgcggt taataaagat gcggaccgcc tggaaggtgc
    26701 gcgtcgcagg atcctgcccc cgctcgcgag tacggacgtt ttgtgccacg atctgcgcca
    26761 gcttgcgggt tgtatcgatt ggactttccg cccgttgcgc aacaatggcg cgcgcaatct
    26821 ggcggctaaa ccgctcttca ccataattct tgattacctc gtgcaaatcc tgttccgacg
    26881 caaccgctat ccactctgcg gcagacatac cgcgcgtcgt atccatacgc atatccaaag
    26941 gggcatcgaa acgaaagctg aaaccgcggc tgccgtcatc gatttgcgga gacgaaatcc
    27001 ccaaatcaaa cagcgcaccg tccaccttgc cgatacccaa accgtccaat gccgtctgaa
    27061 acgaagcaaa accgccatgc acgacaccga cccgtttgtc cgaacgcgcc agctcttctg
    27121 ccacagcaat cgcctgaggg tctttgtcga aaacaatcaa ccgccccgca tcgcccaaac
    27181 gcgacaaaat cagccgggaa tgccctcccc tgccgaacgt accgtccaca tagacaccgt
    27241 cttcgcgcac ggcaagcgca tccaccgcct cattcagcaa gaccgtgata tgccggtaac
    27301 tttctgctcc actcacaatt gcaaatccgt ctgacccaac tggaaggcaa gttcgtcagg
    27361 atcgtcatcc aaagcctgaa ccatctcagc ctcccactgc tcgcgacccc aaagctccaa
    27421 acggttggca cgaccgacca aaacgacttc acggtcgaaa tccaccctct tcctcagtcc
    27481 ggcaggaacc agcacccggc cggcgctgtc ccattccaaa atttccgcgt tatgcagcag
    27541 aagattttga aaccgccgta aaacagggtt atccgccact tttaagttta aaagttgcgc
    27601 cgcaaccttt tcccactccg caacagggta catcaacagc ttgtgtttcg actcgagcgt
    27661 taccactacg gcaggcgtat agaggcgcga cagaatgtca cggaatttgg caggaacagc
    27721 caaccgcccc ttactgtcga tgcttaattc gtgtgcgccg ccgaacatga catgtcccaa
    27781 gccgaaatca aaatcacaag ggtaaaagag acactttgcc ccacaattcc ccaccaatcg
    27841 acactataag aaattttaaa cactcggtca aatcagggca tgaaaactca ttaacatatc
    27901 tgaaattttt attcctttta aaaacaataa gataaaaaat gacgacaacg gcacggcggg
    27961 tgcggtacag aataatcgaa ccaataaaca actatatatg attaatttaa taatataaac
    28021 acaatatata gtattaagat aaagccatga cagcacccgt accaacgtgt aatatgtcgg
    28081 gaaatccaat aaatttacac aagctaacac ttatcatgcc cctccctctc ccctctcccg
    28141 aagcacggca atcctcgctc aaactgcaaa ccctcattgc cgaaaaaatc ggcaaacacg
    28201 gcaactggat tccattttca cgttttatgg aattggtttt atacgctccg caatacggct
    28261 actacaccgg cggcagccat aaaatcggca ataccgggga ttttattacc gcaccgaccc
    28321 tcacctctct gtttgcacag acactggcac gccaacttca agaacttcta tcccaaacgg
    28381 cgggcaatat ctatgaattc ggcgcgggaa ccggacagct tgccgccgat ttgttgggca
    28441 gcatttcgga cggcatcagc cgttactata ttattgaaat atcgccggag ctggcagcac
    28501 gtcagaaaaa cctgattcaa gcccgcgcac cggaagcatc tcaaaaagtt gtccacttga
    28561 ccgcacttcc cgaagcgttt gacggcatca tcatcggcaa cgaagtactc gatgccatgc
    28621 ctgtcgaaat cgtccgtaaa aatgaaggcg gctcattcga gcatgtcggc gtttgcctag
    28681 ataatgaccg ttttacctat tcggcacgac cgctgcacga cttacagcta tctgccttgg
    28741 cttccctcta ttttcctcaa acagattatc cctacaccag cgaactacat ccgcaacaat
    28801 atgcctttat ccgcaccctt gcctccagac tcgaacacgg ctgcatgata ttcatcgact
    28861 acggttttga tgcagcgcag tattaccacc ctcaacgcaa tcaaggtact ctgatcggac
    28921 actaccgaca tcacgttatc cacaatcctt ttgacttcat cggattggcc gacctgaccg
    28981 cgcatgtcaa ctttaccgac attgcccaag ccgggacgga tgccggatta gatttgatag
    29041 gttaccttcc ccaatcccat ttcttattga acctgggcat taccgagcta ttggcacaga
    29101 cggggaaaac gaattcggca gcctatatcc gtgaagctgc tgccgttcag aaactgattg
    29161 accagcatga aatgggcgaa ctgtttaaag tcatcgcatt cggaaaaaat atcggcatcg
    29221 actgggcagg attccgcttc ggcgacatct gccacaaact ctaaccctca tgccgcctga
    29281 atccgcttca gacggcataa actttttaac atttaaaaac agtcaactaa ttcaaaatta
    29341 aaaaatacgg cttgtcaaaa aaacagaaaa acatataata gcgtcttcac gaaacggcga
    29401 attagctcag tcggttagag cagaggaatc ataatccttg tgtccggggt tcgagtccct
    29461 gattcgccac caaattttcg ggggtatagc tcagttggta gagcgcttgc atggcatgca
    29521 agaggtcagc ggttcgatcc cgcttacctc caccagataa aaaagcacag accgtaaaaa
    29581 ggtatgtgct tttttattac ctgattaccg gcaaataaag aataaaccac tgccttcaaa
    29641 acaggcaatc gactttaaac ctatcgcccc gcctgtcctg attttatagt gaattaaatt
    29701 taaaccggta cagcgttggc tcgccttgcc gtactatctg tactgtctgc ggcttcgtcg
    29761 ccttgtcctg atttttgtta attcactata tcagcccgcc agacaaaccc gatccgaata
    29821 atgtcttcag gtcgggttta tggtttcatt cccaacttat ccagcccgac aaccacaata
    29881 ttatgatgac gatgccgaaa acaatgcggt aataggcaaa cgggatatag tttttcttgg
    29941 aaacaaactt cagcagtgct ttgaccgcta ctaaaccgga aacaaaagcg gcaataaagc
    30001 ctatcagaat caaaccgaca tcatgcaggg tgaaaaaccg gtaatgtttc aggacatcat
    30061 aagccgttgc ggcaaccatc atcggaacgg ccaagaaaaa tgaaaactcc gttgccgttt
    30121 tccgctcgat tccccaaagc atcccgccca taatcgtact gcccgagcgg gatgttcccg
    30181 gaatcaaggc aaacacctgc gcgataccga tcatcaaggc atcaatcgga cgcaatgcat
    30241 caacatcggc aattttaggc tctgctcggc tttggcgttt ctccacccac aaaataaaaa
    30301 aaccgcccaa aaccagcatg actgcaacac tcaaggggtt aaacagatac tctttgattt
    30361 gtttgccgaa caacagcccc atcacggcgg caggtataaa agcaatggca agattgagga
    30421 cgaaacggtt ggctttccgg tcttttccta agccgtgcaa cacattgctg aaacgctgcc
    30481 ggtattcaaa cactactgcc aaaaccgcac caagctggat ggcaatttca aaaaccttgt
    30541 ggttgctgtg aaaaccgacc aagttaccga acacaatcaa atgtccggtg ctggaaatag
    30601 gtaaaaattc ggtaaaacct tctaccaagc ccatcatcag ggctttcagg acaatcagaa
    30661 aatccattgc ttggtttctt tcaaatgcgc ggggatgcgg ttatttttgc acaggatggt
    30721 ttgacagttt gcgctccgcc ctgactttgg caaccaattc tttaatcgta tgaacgccgc
    30781 cgtcaaagcc gttattgaag ataacgcggt attttccgcc gacgacaacg gtcggcgtgc
    30841 tgtcgatgcg gtattgttcc gtcagtttct gcatttttaa tgcggcggcg gcagcttcgg
    30901 gggaatcata ggcgcgcatc agttttttgc cgtcaaagcc tttttgagac aaagcccatt
    30961 ttccggcaac agccctgttt tccaagcgga ttttttgttc gtaaactgct ttaaacacgg
    31021 cagggtttgc ctgatatttc aaacccgaca aattgacggc agccgccatt cttgccagac
    31081 cgagcatttc aagctgccag accacgtgct ccgtcctcaa ataggcatca gacggcaatg
    31141 ccttgcccag tttcaataac aaaggatcga aatgatggca atgtacgcag aaatagccga
    31201 aaaattccaa aacctcaatt ttacccgact gttcttgagg aatgggttta tccaacacaa
    31261 gatagtcttc cccttccgtc agggcatatg cctgcgcgga caacactgcc gacagcagca
    31321 gcggcaacag atgtttgagc ttcataatta tttgctttcg atagaacgga tcaggctggc
    31381 gacttcatgt tttttcaact cgtcctgcat ttttttcacc gcatcggcag acatattgcc
    31441 gctttgcacc cggtaaagcg ttttatgtcc cgcctgataa ccgaccacct tggaagatat
    31501 gcccaagatt gccagtttgg cacgctgccc ttccgcgctc cggcggtcgg catacgcgcc
    31561 catttgcaga taatgcgttg cttccgcctt gtcggacgtt ttcattttct gcacttcttt
    31621 ggcggcggca ctgcgcgctt tttcgatgct gccgctgttg aggatttgtt ccggggtcgg
    31681 tttgggtgca actttttcct tttccgcctt tttctcttct tttggagctt ttttctctgt
    31741 ttctttagac ggttttaccg cttgtttttt aaccgtttcg gcatctttct tctgcgcttt
    31801 ttccctgacg gtttgttcac gctcttccgt cagcactttc ttacgcactg cctgtccgtc
    31861 cggctcttcc cgttccggct cgcccgcctt ttcttcaacc tcgtcggctt tatcggcagc
    31921 tttttccgca tccgactgct ctgcctctgt cgcagcatcc ggttcggaca aggcgttttg
    31981 atcggccggt tcaggttgga tgtcttcctt aggctggttt ttcggtttca ggatttccgt
    32041 ttctgcaggc tgcttcgacg gaaccgggat tttgaacgca ttttgaccgc tctggttcag
    32101 ataaaacaaa ataccggcaa taatgaccgt cgccagtatc aaaccgaaga aaaaaccgga
    32161 cagacctttt ccggattggg aaaatttgtt cataaacata ccttaatgtg tttcagacgg
    32221 cattaacgcc gtttgcttac gggcggatat tctaacaata tcgccatatt tgggcaaaac
    32281 ctgcttccat tcccgttcct ataaagcgcg acggaaacct atggctttca tgacgtggct
    32341 tctgccgact tcttcgtcgc ccgccaaatc cgccaatgta cgcgccacgc gcataatgcg
    32401 gtggaagctg cgggcggaaa gggagagttt ttccagcagg ccgcccaatg cttcctgcgc
    32461 ttctttttga atgcgggctt gggaatcgag ttcgctgacg ctcaaagcgg cattcacttt
    32521 gccttgccgt gcgtattgtt tgtctctggc ggcgataacg cgttccaaaa cggacgcgct
    32581 gctttcccct gcttcctgct gcatcagttc ggcggcggac aggctcggga cttcgatggt
    32641 caaatcgatg cggtcgagca gcgggccgga aatcttgctg cggtaacgcg cgacgctttc
    32701 gggcgtgcaa cggcagggtt tgatgggatg cccgagataa ccgcaaggac aagggttcat
    32761 ggcggcaaca agttggaatt tggcaggata gacggcttgg cgcgccgcgc gggaaatgtg
    32821 gatttcgccg ttttccaacg gttcgcgcaa aacttctaaa actttgcggt cgaactcggg
    32881 cagctcgtcc aaaaacaata cgccgtggtg cgccaatgaa atttcgcccg ggcgcggatc
    32941 cgaaccgccg ccgaccatag ccgccgcgct ggcgctgtga tgcggactgc ggaaaggacg
    33001 gttgctgtcg agttgttgtt ggtggttggg caggagcgaa cgcaatgccc aaacttctac
    33061 caattcgtct tcggtcagcg gcggcaggat gccgggcagc cgttgggaga gcatagactt
    33121 gcccgttccc ggcggaccca tcatcaagag gctgtgtccg cctgcggcag cgatttccaa
    33181 agcaaggcgc gcggtgtgct gacctttcac atcgcacaaa tcaggttgtc cgccatgttc
    33241 agacggcatt tgcggtacgg aacattcggt ttgcgccaaa ggttcgatgc cgttcaaatg
    33301 ggcggcgact tcacccaaag agcgcgcgcc gtaaacggta atgccgcgca tcacggcggc
    33361 ttgtcctgcg ttttcttcag gcaaaacaaa tgcacgtttt gcctgcatac cctgccacgc
    33421 catcgccaac gcgccgcgca cggggcgcaa cagccccgac agtgccaatt cccccgcaaa
    33481 ctcgtattcc gccagttttt cgggcgcaac ctgccccgat gcggcaagga tgccgattgc
    33541 aatcggcaaa tcgaaacgcc ccgactcttt gggcaggtcg gcgggggcga ggttgacggt
    33601 gatttttttg gcggggaatt caaaaccgct ttgaataatg gcggcacgga cgcggtcgcg
    33661 actttccttt acttccgtat cgggcagccc gacgatgttg aaatgcggca ggccgttggc
    33721 aaggtgggct tccacttcga ccaacggcgc attcatacca ctcaaggcgc ggctgtaaac
    33781 caaggcaagc gacatatttc agacggcctt attcgccggc ttcggtttgc tgcctgattt
    33841 cggcgacggc ttcttcggca gcggcttcag ccgcttccaa tgctgcccgt tcgggatttt
    33901 gcgcggcttc gagttttgcc aaacgcgctt ccaaagccgc cagtttggta cgggttttga
    33961 ttaaaacctg ctgctggatg tcgaattctt cgcgcgtaac cagatccata cggttgaacg
    34021 cgccgcccag catcgcctta atattttttt ccacatcttt ggcagggctg ttggcgatgg
    34081 tttcgctgat tttcgagccg acttcctcaa aaagctgctt gccgaacata atctgtatcc
    34141 ttcctgaaca tatcaaattc aatcggctat tgtataagga aaaatgccgt ctgaaaacgg
    34201 gcggcggata atcggcaaaa cataccgcgc ctcctttgcg gttgctaaat tacacctcaa
    34261 aacacctgcc gcctgaaaac ggatttcata ttccgcgcaa cgcccgatta caagacacta
    34321 caaaacaata tgctgtttta aatgattttt ccgacgcgca tcgtttcaaa ctcggctttt
    34381 taagccatta agtgctttgc aaacaacaga aattgggtta tgctgaaacg gattatttac
    34441 aatttcatat agttttatta catatcttat tgtgattgaa gataatttat ccgaatcccc
    34501 ctttcgggta tccggatttt ccgttgtact tttattagaa aaacatttca ggcgcaagtt
    34561 gcttgcaatt tcaaagcctg taattctgaa gttttagacg aggaatagca catgaatccc
    34621 atgtacatta cttttgcaat ctatctggtt gcagtccttc tcatcgggct tgccgcctat
    34681 ttttctacgc gcaatttcga cgattacatt ttgggcgggc gcagcctggg cccgtttgtt
    34741 accgcgatgt cggcaggcgc gtccgatatg tccggctggc tgctgatggg tctgccgggc
    34801 gcgatttacc tgagcggttt gaatgaggct tggattgcca tcggcctctt ggtcggcgcg
    34861 tatttcaact ggctcttggt ggcgggccgc ctgcgcgtgc ataccgaata cgccaacaat
    34921 gcgctgacgc tgccggatta tttcttccac cgcttcggcg cgggcggaca cttgatgaaa
    34981 gtggtttccg cactgattat cctgttcttc ttcacgattt attgcgcctc gggcattgtg
    35041 gcgggcgcaa ccctgttcca aagcctgttt gaaggtatga cttacaatca ggcaatgtgg
    35101 ctgggcgcgg gcgcgaccat cgcctatacc ttcttgggcg gcttcttggc ggtcagctgg
    35161 acggatacgt tgcaggcttc tttgatgatt ttcgcgctga ttttaacgcc tgtgatggtc
    35221 tatctgggct tgggcggcgc ggaacagatg tctgccgcga ttcaaaacgt cgccgcaggc
    35281 acgggcaaag aatacggcag cctgtttgcc ggtacgaccg tcatcggcat catttccacc
    35341 gccgcctggg gcttgggcta tttcggacag ccgcacattt tggcgcgctt tatggcggcc
    35401 gaaagcgcga aatcgctggt atccgcacgc cgcatcggta tgacttggat ggcgttgtgc
    35461 cttgcgggcg cggtagcggt cggttatttc ggcattgcgt atttcggtgc aaatcccgac
    35521 aaagtttctt ctatgagcgg caaccacgaa cgcatcttca tcgcgctttc caccctgctg
    35581 ttcaaccctt ggatcgccgg cattattttg agcgcgattt tggcggcggt gatgtccacc
    35641 ctgtcctgcc agcttttggt ttgctccagt gcgattaccg aagacttcta caaaggcttt
    35701 ttgcgtaaaa acgcgcaaca gtcggaactg gtatgggtcg gccgcctgat ggtcttggcg
    35761 attgccgtga tttccatcct gattgcttcc gatccgaaca gcaaagtatt gggactggtg
    35821 tcttatgcgt gggcaggttt cggtgcggca ttcggcccga ttgtgattct gtccgtattg
    35881 tggaaacgca tcactgccta cggcgcgttg tcgggtatgg ttgcaggtgc cttaaccgta
    35941 gtcgtatggg cggaatgggt gaaaaaaccg gctcaagcgg caggcgaaag cggcttgttg
    36001 acgatgtacg aaatcgtccc gggctttatt gtttgcttga tcgttgccgt gttggtttcc
    36061 ctgttcaaca aagaaccttc gcgcgaaacc caagaacgct ttgaaaaagc cgacgcagat
    36121 taccgcgccg cccgataaac ccctttcaga cggcacggca tcccggttcg tgccgtctga
    36181 acccgtttcc gggtcaaaca caaataaacc ttgcctgcat acaggcctta tttgtgtttt
    36241 acccgtttca ttttcaaaga aacggaagca cctaccctgc ccgcatttga agccttgtat
    36301 ttcatctgcg gatttattac gatattaccg tattcaggcc gcaccgatgc cgcctgcccc
    36361 ccgaaaaact ttggagaatc caaaaaatgt ttcattttgc atttccggca caaactgccc
    36421 tgcgccaagc gataaccgat gcctaccgcc gtaatgaaat cgaagccgta caggatatgt
    36481 tgcaacgtgc acagatgagc gacgaagagc gcaacgccgc ctccgagctt gcccgccgtt
    36541 tggttaccca agtccgcgcc ggccgcacca aagccggcgg cgtagatgcg ctgatgcacg
    36601 agttttcact ctccagcgaa gaaggcatcg cgctgatgtg tctggcagaa gccctgctgc
    36661 gtatccccga caacgccacg cgcgaccgcc tgattgccga caagatttca gacggcaact
    36721 ggaaaagcca tttgaacaac agcccttccc tcttcgtcaa cgccgccgcc tggggcctgc
    36781 tgattaccgg caaactgacc gccacaaacg acagacaaat gagttccgca ctcagccgcc
    36841 tgatcagcaa aggcggcgca ccgctcatcc gccaaggcgt aaattacgcc atgcgcctgt
    36901 tgggcaaaca gttcgtaacc ggacagacca ttgaagaagc cctgcaaaac ggcaaagaac
    36961 gcgaaaaaat gggctaccgc ttctccttcg atatgttggg cgaagccgcc tacacccaag
    37021 ccgatgccga ccgctactac cgcgactatg tcgaagccat ccacgccatc ggcaaagatg
    37081 cggcaggaca aggcgtttac gaaggcaacg gcatttccgt caaactctcc gccatccatc
    37141 cgcgctactc gcgcgcccaa cacggccgtg tgatgggcga actgttgccg cgcctgaaag
    37201 agctgttcct tttgggtaaa aaatacgata tcggtatcaa catcgatgcc gaagaagcca
    37261 accgtctgga gctgtctttg gatttgatgg aggcactcgt ttccgatccc gatttggcag
    37321 gctacaaagg tatcggtttc gttgttcaag cctaccaaaa acgttgtccg ttcgttatcg
    37381 actacctgat cgaccttgcc cgccgcaaca accaaaaact aatgatccgc ctcgtcaaag
    37441 gcgcgtattg ggacagcgaa atcaaatggg cgcaagtgga cggcttgaac ggctatccga
    37501 cctacacccg caaagtccac accgacatct cctacctcgc ctgcgcgcgc aaactgcttt
    37561 ccgcgcaaga cgcggtattt ccgcaattcg ccacccacaa cgcctacacc ttgggcgcaa
    37621 tctaccaaat gggcaaaggc aaagactttg aacaccaatg tctgcacggc atgggcgaaa
    37681 ccctgtacga ccaagtcgtc ggcccgcaaa acttaggccg ccgcgtgcgc gtgtacgccc
    37741 cggtcggcac acacgaaacc ctgctcgcct acttggtgcg ccgcctgttg gaaaacggcg
    37801 cgaactcgtc tttcgtcaac caaatcgtcg ataaaaacat cagcatcgac acgctcatcc
    37861 gcagcccgtt cgacaccatc gccgaacaag gcatccacct gcacaacgcc ctgccgctgc
    37921 cgcgcgattt gtacggcaaa tgccgtctga actcgcaagg cgtggacttg agcaacgaaa
    37981 acgtattgca gcagcttcaa gaacagatga acaaagccgc cgcgcaagac ttccacgccg
    38041 catccatcgt caacggcaaa gcccgcgatg tcggcgaagc gcaaccgatt aaaaaccctg
    38101 ccgaccacga cgacatcgtc ggcacagtca gctttgccga tgccgcgctt gcccaagaag
    38161 cggttggcgc agccgttgcc gcgttccccg aatggagtgc gacacctgcc gccgaacgcg
    38221 ccgcctgcct gcgccgtttc gccgacctgc tcgaacagca cacccccgcg ctgatgatgc
    38281 ttgccgtgcg cgaagcaggc aaaacgctga acaacgccat tgccgaagtg cgcgaagccg
    38341 tcgatttctg ccgctactac gcaaacgaag ccgaacatac cctgcctcaa gacgcaaaag
    38401 ccgtcggcgc gattgtcgcc atcagcccgt ggaacttccc gctcgccatc tttaccggcg
    38461 aagtcgtttc cgcattggcg gcaggcaaca ccgtcatcgc caaacccgcc gaacaaacca
    38521 gcctgattgc cggttatgcc gtttccctca tgcacgaagc cggcatcccg acttccgccc
    38581 tgcaactcgt cctcggcgca ggcgacgtgg gtgcggcatt gaccaacgat gcccgcatcg
    38641 gcggcgtgat tttcaccggc tcgaccgaag tggcgcgcct gatcaacaaa gcccttgcca
    38701 aacgcggcga caatcccgtc ctgattgccg aaaccggcgg acaaaacgcc atgattgtcg
    38761 attccaccgc acttgccgaa caagtctgcg ccgacgtatt gaactccgcc ttcgacagcg
    38821 cgggacaacg ctgctccgcc ctgcgtatct tgtgcgtcca agaagacgtt gccgaccgta
    38881 tgctcgacat gatcaaaggc gcgatggacg aactcgtcgt cggcaaaccg attcagctca
    38941 ctaccgatgt cggccccgtc atcgatgccg aagcacagca aaacctgttg aaccacatca
    39001 acaaaatgaa aggcgttgcc aagtcttacc acgaagtcaa aaccgctgcc gatgtcgatt
    39061 ccgaaaaatc cacgttcgtc cgccccatcc tgtttgaatt gaacaacctc aacgaactgc
    39121 aacgcgaagt cttcggtccc gtcctgcacg tcgtccgcta ccgcgccgac aaactcgaca
    39181 gcgtcatcga ccaaatcaac agcaaaggct acgccctgac ccacggcgta cacagccgca
    39241 tcgaaggcac agtacgccac atccgcagcc gcatcgaagc cggcaacgtt tacgtcaacc
    39301 gcaacatcgt cggcgcagtc gtcggcgtac agcccttcgg cggacacggt ctgtccggca
    39361 caggccccaa agcaggcggt tcgttctacc tgcaaaaact gacccgcatc cccgaatggg
    39421 ttgccccgac cctgagccaa atcggacagg cagacgaagc cgcactcaaa cgcctcgaag
    39481 cactggttca caaactgccg ttcaacgccg aagagaaaaa agccgcagcg gccgctttgg
    39541 gacacgcccg catccgcacc ctgcgccgtg ccgaaaccgt ccttaccgga ccgaccggcg
    39601 agcgcaacag catctcatgg cacgcgccca aacgcgtttg gatacacggc ggcagcacgg
    39661 ttcaagcctt tgccgcactg accgaacttg ccgcctccgg catacaggca gtggtcgaac
    39721 ccgacagccc cttggcttcc tacactgccg acttggaagg tctgctgctg gtcaacgaca
    39781 aacccgaaac cgccggcatc agccacgttg ccgccctgtc gcctttggac agcgcgcgca
    39841 aacaggaact tgccgcccac gacggcgcac tcatccgcat cctcccttcg gaaaacggac
    39901 tcgacatcct gcaagtgttt gaagaaatct cttgcagcgt caacaccaca gccgccggcg
    39961 gcaacgccag cctgatggcg gttgccgact gattttaccg aaatccccgg gcgcggcccg
    40021 tgaaccaatg ccgtctgaaa acctttcaga cggcattttt ataatggatt aacaaaaatc
    40081 aggacaaggc gacgaagccg cagacagtac aaatagtacg a
//
LOCUS       DAWFIT010000008        38989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFIT010000008 DAWFIT010000000
VERSION     DAWFIT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.008"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGQ85_000367"
     CDS             <1..69
                     /locus_tag="WGQ85_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0749868.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            87..398
                     /gene="rpsJ"
                     /locus_tag="WGQ85_000368"
     CDS             87..398
                     /gene="rpsJ"
                     /locus_tag="WGQ85_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0749869.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            689..1741
                     /locus_tag="WGQ85_000369"
     CDS             689..1741
                     /locus_tag="WGQ85_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ0749870.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            1750..2433
                     /locus_tag="WGQ85_000370"
     CDS             1750..2433
                     /locus_tag="WGQ85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ0749871.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            2652..3296
                     /gene="rplC"
                     /locus_tag="WGQ85_000371"
     CDS             2652..3296
                     /gene="rplC"
                     /locus_tag="WGQ85_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0749872.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            3296..3916
                     /gene="rplD"
                     /locus_tag="WGQ85_000372"
     CDS             3296..3916
                     /gene="rplD"
                     /locus_tag="WGQ85_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0749873.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            3913..4227
                     /gene="rplW"
                     /locus_tag="WGQ85_000373"
     CDS             3913..4227
                     /gene="rplW"
                     /locus_tag="WGQ85_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0749874.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            4233..5066
                     /gene="rplB"
                     /locus_tag="WGQ85_000374"
     CDS             4233..5066
                     /gene="rplB"
                     /locus_tag="WGQ85_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0749875.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQITRSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            5073..5351
                     /gene="rpsS"
                     /locus_tag="WGQ85_000375"
     CDS             5073..5351
                     /gene="rpsS"
                     /locus_tag="WGQ85_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0749876.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            5360..5689
                     /gene="rplV"
                     /locus_tag="WGQ85_000376"
     CDS             5360..5689
                     /gene="rplV"
                     /locus_tag="WGQ85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0749877.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            5699..6391
                     /gene="rpsC"
                     /locus_tag="WGQ85_000377"
     CDS             5699..6391
                     /gene="rpsC"
                     /locus_tag="WGQ85_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0749878.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            6375..6791
                     /gene="rplP"
                     /locus_tag="WGQ85_000378"
     CDS             6375..6791
                     /gene="rplP"
                     /locus_tag="WGQ85_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0749879.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            6791..6982
                     /gene="rpmC"
                     /locus_tag="WGQ85_000379"
     CDS             6791..6982
                     /gene="rpmC"
                     /locus_tag="WGQ85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0749880.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGQ85_000380"
     CDS             6982..7245
                     /gene="rpsQ"
                     /locus_tag="WGQ85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0749881.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            7467..7835
                     /gene="rplN"
                     /locus_tag="WGQ85_000381"
     CDS             7467..7835
                     /gene="rplN"
                     /locus_tag="WGQ85_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0749882.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7847..8170
                     /gene="rplX"
                     /locus_tag="WGQ85_000382"
     CDS             7847..8170
                     /gene="rplX"
                     /locus_tag="WGQ85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0749883.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            8180..8719
                     /gene="rplE"
                     /locus_tag="WGQ85_000383"
     CDS             8180..8719
                     /gene="rplE"
                     /locus_tag="WGQ85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0749884.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            8722..9027
                     /gene="rpsN"
                     /locus_tag="WGQ85_000384"
     CDS             8722..9027
                     /gene="rpsN"
                     /locus_tag="WGQ85_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0749885.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            9043..9435
                     /gene="rpsH"
                     /locus_tag="WGQ85_000385"
     CDS             9043..9435
                     /gene="rpsH"
                     /locus_tag="WGQ85_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0749886.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            9449..9982
                     /gene="rplF"
                     /locus_tag="WGQ85_000386"
     CDS             9449..9982
                     /gene="rplF"
                     /locus_tag="WGQ85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0749887.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            9996..10349
                     /gene="rplR"
                     /locus_tag="WGQ85_000387"
     CDS             9996..10349
                     /gene="rplR"
                     /locus_tag="WGQ85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0749888.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            10368..10886
                     /gene="rpsE"
                     /locus_tag="WGQ85_000388"
     CDS             10368..10886
                     /gene="rpsE"
                     /locus_tag="WGQ85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0749889.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            10879..11064
                     /gene="rpmD"
                     /locus_tag="WGQ85_000389"
     CDS             10879..11064
                     /gene="rpmD"
                     /locus_tag="WGQ85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0749890.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            11066..11500
                     /gene="rplO"
                     /locus_tag="WGQ85_000390"
     CDS             11066..11500
                     /gene="rplO"
                     /locus_tag="WGQ85_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0749891.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            11512..12822
                     /gene="secY"
                     /locus_tag="WGQ85_000391"
     CDS             11512..12822
                     /gene="secY"
                     /locus_tag="WGQ85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0749892.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            12827..13045
                     /gene="infA"
                     /locus_tag="WGQ85_000392"
     CDS             12827..13045
                     /gene="infA"
                     /locus_tag="WGQ85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0749893.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGQ85_000393"
     CDS             13066..13179
                     /gene="rpmJ"
                     /locus_tag="WGQ85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0749894.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            13245..13607
                     /gene="rpsM"
                     /locus_tag="WGQ85_000394"
     CDS             13245..13607
                     /gene="rpsM"
                     /locus_tag="WGQ85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0749895.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            13627..14022
                     /gene="rpsK"
                     /locus_tag="WGQ85_000395"
     CDS             13627..14022
                     /gene="rpsK"
                     /locus_tag="WGQ85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0749896.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            14042..14662
                     /gene="rpsD"
                     /locus_tag="WGQ85_000396"
     CDS             14042..14662
                     /gene="rpsD"
                     /locus_tag="WGQ85_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0749897.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            14688..15674
                     /gene="rpoA"
                     /locus_tag="WGQ85_000397"
     CDS             14688..15674
                     /gene="rpoA"
                     /locus_tag="WGQ85_000397"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0749898.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            15698..16066
                     /gene="rplQ"
                     /locus_tag="WGQ85_000398"
     CDS             15698..16066
                     /gene="rplQ"
                     /locus_tag="WGQ85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0749899.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            16213..16926
                     /gene="minC"
                     /locus_tag="WGQ85_000399"
     CDS             16213..16926
                     /gene="minC"
                     /locus_tag="WGQ85_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0749900.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            16955..17770
                     /gene="minD"
                     /locus_tag="WGQ85_000400"
     CDS             16955..17770
                     /gene="minD"
                     /locus_tag="WGQ85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0749901.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            17774..18037
                     /gene="minE"
                     /locus_tag="WGQ85_000401"
     CDS             17774..18037
                     /gene="minE"
                     /locus_tag="WGQ85_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0749902.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            18041..18961
                     /gene="oxyR"
                     /locus_tag="WGQ85_000402"
     CDS             18041..18961
                     /gene="oxyR"
                     /locus_tag="WGQ85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0749903.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(18958..19149)
                     /locus_tag="WGQ85_000403"
     CDS             complement(18958..19149)
                     /locus_tag="WGQ85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749904.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(19291..22128)
                     /locus_tag="WGQ85_000404"
     CDS             complement(19291..22128)
                     /locus_tag="WGQ85_000404"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0749905.1"
                     /translation="MLDKYNPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTVWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPSEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDETG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATLHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGQ85_000405"
     CDS             complement(22225..23034)
                     /gene="zupT"
                     /locus_tag="WGQ85_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0749906.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(23215..24471)
                     /locus_tag="WGQ85_000406"
     CDS             complement(23215..24471)
                     /locus_tag="WGQ85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0749907.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(24550..25941)
                     /locus_tag="WGQ85_000407"
     CDS             complement(24550..25941)
                     /locus_tag="WGQ85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0749908.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGQ85_000408"
     CDS             complement(26298..27074)
                     /gene="lpxA"
                     /locus_tag="WGQ85_000408"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0749909.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(27117..27797)
                     /locus_tag="WGQ85_000409"
     CDS             complement(27117..27797)
                     /locus_tag="WGQ85_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0749910.1"
                     /translation="MERYKNAVGKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDGNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQHNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGQ85_000410"
     CDS             complement(28108..28557)
                     /gene="fabZ"
                     /locus_tag="WGQ85_000410"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0749911.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGQ85_000411"
     CDS             complement(28593..29636)
                     /gene="lpxD"
                     /locus_tag="WGQ85_000411"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0749912.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDTSVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(29669..30169)
                     /locus_tag="WGQ85_000412"
     CDS             complement(29669..30169)
                     /locus_tag="WGQ85_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0749913.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLKDAKKAQAEEKWCGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGQ85_000413"
     CDS             complement(30235..32628)
                     /gene="bamA"
                     /locus_tag="WGQ85_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0749914.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGQ85_000414"
     CDS             complement(32685..34025)
                     /gene="rseP"
                     /locus_tag="WGQ85_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0749915.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(34060..35295)
                     /locus_tag="WGQ85_000415"
     CDS             complement(34060..35295)
                     /locus_tag="WGQ85_000415"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0749916.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(35300..36097)
                     /locus_tag="WGQ85_000416"
     CDS             complement(35300..36097)
                     /locus_tag="WGQ85_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0749917.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(36100..36846)
                     /locus_tag="WGQ85_000417"
     CDS             complement(36100..36846)
                     /locus_tag="WGQ85_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0749918.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGQ85_000418"
     CDS             complement(36902..37459)
                     /gene="frr"
                     /locus_tag="WGQ85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0749919.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(37640..38767)
                     /locus_tag="WGQ85_000419"
     CDS             complement(37640..38767)
                     /locus_tag="WGQ85_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0749920.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(38837..38912)
                     /locus_tag="WGQ85_000420"
     tRNA            complement(38837..38912)
                     /locus_tag="WGQ85_000420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38877..38879),aa:Thr,seq:cgt)
BASE COUNT         9365 a         8721 c        10583 g        10320 t
ORIGIN      
        1 ttgcgctttg cgattcgcga aggcggccgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaat tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag
      121 cttatgatta cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta
      181 ccggtgcagt tgtaaaaggc ccgattcctt tgccgaccaa aatcgaacgt ttcaacattc
      241 tgcgttctcc gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccacttgc
      301 gcctgatgga catcgtggat tggaccgaca aaactaccga tgcgctgatg aagctggatt
      361 tgccggccgg tgttgatgta gaaatcaaag tccaataatt agggctgttg aaaatcccca
      421 agcaattaat gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca
      481 gatttaaggc aaaacaacaa aatcaaaaaa cagtgcggtc ggcgaaatcg gcttattttg
      541 tgcctcaaga attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat
      601 agccttccga aggcttgccc ccccccgaag aaaggtagaa tcgggcttgg tttgggcaga
      661 agcatccatc tgattctaag gatggatgat gttttattct caagtaaata aaggaaagat
      721 gatgagctta cttaatgtct ttaaagatat tttaaacaac aaagaattga attcggaaga
      781 acggcatgat ttggagcggg cggttaagat cttgcaggat acgcatgtca atattttgat
      841 taccggagcc accggttgcg gaaaatcatc caccatcaat gccttgttca gcatagatca
      901 ggcaacggga aagagtgatg gactagtaaa ggaagtcgca aaagtaggcg tgggtgtaga
      961 tcctgaaact atggatattg ccaaatatga attggagaat atgacgattt gggatagtcc
     1021 gggcttgggc gacggagaga aggcagacca aaagcaccgg cgcaatatca tcaataagct
     1081 gctggagaaa gacagtaacg gcaatgccgt catcgacttg gttttggtgc tgcttgacgg
     1141 cggctccaga gacttgggga catcatacga actgattaac aatgtaatca tcaatacatt
     1201 gaagaagagt ggggaaggcc gtacagaccg gttgctggtt gcgattaatc agtgtgatat
     1261 ggcgatgaag gggcggaatt gggatgcgga agccggccgt ccgaacgccg ccttggaaaa
     1321 ctttttggag gagaaagtgt gttcgacacg caagcgcatt caggaagcta ccggcgttac
     1381 ggtcaatccg atttattttt cagcaggctt taagagtgag gatgaggagc agcgtcccta
     1441 taacataacc cgcctgttgc gttacatcat tacggctatg ccgtctgaaa aacgggtgat
     1501 tgtggcagag aagattaatg tggaagaaat cgaacgagat accagccgcc gcaaacagga
     1561 ggagcggcaa gaaatcgaca gtagtatcgg cagtgcgctg gagaaaatat tgggggctgc
     1621 gggaggcgtg gttaaagatg ttgtttcaac gatagcaagc ggtgtcggga cgctgatcgg
     1681 cggtgctgca aaagcgattg gaggggcgat tagcacggtt gcttcttttt ttggctggta
     1741 ggagcgcata tgcatcgtta ccgtatttct gaaatcgggc aaaaagtcac agcagccggt
     1801 ttccgtcatt tggatgtctt gcttgtcggt gcgacaggtg tcggcaagtc ctcaacctta
     1861 aatgccttgt ttggcgaaca aaaagccaag gtaggcacag gcgtggatcc cgaaacgcaa
     1921 ttggtcgaaa gctatatgct gaacgatgtg ttgcggtttt gggacagcgc aggtttgggc
     1981 gatgggaaag aagccgaccg cgcccatcgg caaaaactga ttgatgtcct gtctaaaacc
     2041 tatactcatt cggatgggca gtggggctgg atagatttgg tgttcgttat ccttgacggc
     2101 agctcccgcg atttgggtac ggcctatgat ttgttgaggg atgttatcct taaaatgatt
     2161 gatccggata gggttattgt tgcaatcaat caggcggata tggcgatgaa aggacgttat
     2221 tgggataagg tgctgcatca gccgcagccg aatttgcaac agtttctaga cgagaaggcc
     2281 gaatcggtac aaaaaaggat tctggaagca accggtttgc agatttccaa acccgtttac
     2341 tattcggcat acgaaaacta ccatctcaaa gaaataatgg atgcagtcat caaccatatc
     2401 ccggtttgtc ggagaaagat gcatacgccg tgatggtcgg ataccgactc ctttaaaagc
     2461 atccgaccct atgccgtctg aagattcaaa acgcttcaga cggcatattg aagatatttt
     2521 tgatattttt gttgatattt ctttgacttg tcagatataa tgtctgactt ggtacattcg
     2581 taccaagttt aactttgtct gaaagacagg ccaatcgtag cctgtccctt tactttaaaa
     2641 ggaaaataat catgacttta ggtctggttg gacgcaaagt tggtatgacc cgcgtgttcg
     2701 acgaacaggg tgtttctgtt ccggtaaccg ttttggatat gtctgccaac cgcgttacac
     2761 aagtaaaatc caaagatact gacggctata ctgccgttca agttaccttt ggtcagaaaa
     2821 aagccaatcg tgtcaacaaa gccgaagccg ggcactttgc aaaagcaggt gttgaagccg
     2881 gtcgcggttt gattgagttt gctttgactg aagaaaaact ggctgaattg aaagctggtg
     2941 acgaaatcac cgtttctatg tttgaagtcg gtcaactggt cgatgtaacc ggtacctcta
     3001 aaggtaaagg tttctccggc acgatcaaac gtcataactt cggtgcccaa cgtacttccc
     3061 acggtaactc ccgttctcac cgtgttccag gctctatcgg tatggcgcaa gacccgggtc
     3121 gcgtgttccc cggtaaacgc atggccggcc aatacggcaa caccaaagca actgttcaaa
     3181 aattggaagt tgtccgtgtt gatgcagaac gccaactgct gttggttaag ggtgctgttc
     3241 cgggtgcggt caacagcgat gttgtagttc gtcccagcgt gaaagtaggt gcgtaatgga
     3301 attgaaagta attgacgcta aaggacaagt ttcaggcagc ctgtctgttt ctgatgcttt
     3361 gttcgcccgc gaatacaatg aagcgttggt tcatcagctg gtaaatgcct acttggcaaa
     3421 cgcccgctct ggtaaccgtg ctcaaaaaac ccgtgccgaa gtaaaacact caaccaaaaa
     3481 accatggcgt caaaaaggta ccggccgcgc ccgttccggt atgacttcat ctccgctgtg
     3541 gcgtaaaggt ggtcgcgcgt tcccgaacaa acccgacgaa aacttcactc aaaaagtaaa
     3601 ccgcaaaatg taccgtgccg gtatggcgac tattctgtcc caattgactc gtgacgagcg
     3661 tttgtttgcg attgaggcgt tgactgctga aactcctaaa accaaagttt ttgctgaaca
     3721 agtgaaaaat ctgggtctgg agcaagtgtt gtttgtaacc aaacagctcg acgagaatgt
     3781 ttacttggct tcacgcaact tgccaaacgt gttggttttg gaagctcaac aagttgatcc
     3841 ttacagcttg ctgcgttaca aaaaagtaat catcactaaa gatgcagttg cacaattaga
     3901 ggagcaatgg gtatgaatca acaacgtttg actcaagtga ttttggcacc tatcgtttct
     3961 gaaaaaagca acgtattggc tgaaaaacgc aaccaaatga cgtttaaagt cttggcaaat
     4021 gcaaccaaac ctgaaatcaa agctgctgtt gagctgctgt ttggtgttca agttgcttct
     4081 gtaactaccg ttacaactaa aggcaaaact aagcgttttg gtcgtacttt gggccgccgc
     4141 agcgatgtta aaaaagctta tgtaagcttg gctgccggtc aagagttgga tttggaagcc
     4201 gctgctgcag ctgcagataa ggaataaaca aaatggcaat cgttaaaatg aagccgacct
     4261 ctgcaggccg tcgcggcatg gttcgcgtgg taacagaagg tttgcacaaa ggtgcacctt
     4321 atgcgccctt gcttgaaaag aaaaattcta ctgccggccg taacaataat ggtcatatca
     4381 ccacccgtca caaaggcggc ggtcataaac accattaccg tgttgtagac tttaaacgta
     4441 acaaagacgg cattcctgcc aaagtagagc gtatcgaata cgatcctaac cgtactgcct
     4501 tcatcgcact gttgtgctat gcagacggcg agcgtcgtta catcattgct cctcgcggta
     4561 ttcaagccgg tgctgtattg gtttccggtg ctgaagctgc catcaaagta ggcaacaccc
     4621 tgccgattcg caacattccc gttggtacga ctatccactg tatcgaaatg aaacccggta
     4681 aaggtgctca aatcacacgt tctgccggtg cttctgcggt attgctggct aaagaaggcg
     4741 cgtacgctca agtccgtctg cgctctggcg aagtccgtaa aatcaacgta aattgccgtg
     4801 cgaccattgg tgaagttggt aacgaagagc aaagcctgaa aaaaatcggt aaagccggtg
     4861 ccaaccgttg gcgcggtatt cgtccgaccg tacgcggtgt tgtcatgaat cctgtcgatc
     4921 acccgcatgg tggtggtgaa ggccgtacgg gcgaggcccg cgaaccggtc agcccatggg
     4981 gtactcctgc taaaggctac cgcactcgta ataacaaacg cacggataac atgattgttc
     5041 gtcgccgtta ctcaaataaa ggttaattta gtatggctcg ttcattgaaa aaaggcccat
     5101 atgtagacct gcatttgctg aaaaaagtag atgctgctcg cgcaagcaac gacaaacgcc
     5161 cgattaaaac ctggtctcgt cgttctacca ttctgcctga ttttatcggt ctgaccattg
     5221 ctgtgcacaa cggccgcacc catgtgcctg tgtttatcag cgacaatatg gttggtcata
     5281 aattaggcga attctcattg acccgtacct ttaaaggcca cttggccgat aaaaaggcta
     5341 aaaagaaata aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc
     5401 tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa
     5461 tattttggct ttcagtccta aaaaaggtgc cgagctgatt aaaaaagtat tggagtcagc
     5521 tattgctaat gccgagcaca ataacggtgc ggacattgat gaactgaaag tggtaactat
     5581 ctttgttgac aaaggcccaa gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa
     5641 ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat
     5701 gggacaaaag attaacccta caggctttcg cctggcggta actaaagact gggcttcaaa
     5761 atggtttgct aaaagcaccg acttttctac tgttttgaag caggatatcg atgttcgcaa
     5821 ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga gtggttattg aacgccctgc
     5881 aaaatctgca cgcattacca ttcactccgc tcgtccgggt gtggttatag gtaaaaaagg
     5941 tgaggatatc gaggttttga aacgtgactt gcaagtcttg atgggtgtac ctgttcatgt
     6001 aaatattgaa gagattcgcc gtcctgagtt ggatgctcaa attattgctg acggtattgc
     6061 ccagcagttg gaaaagcgcg ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc
     6121 aatgcgttct ggtgctaaag gcattaagat tatgacttca ggccgtctga atggtgcgga
     6181 tattgcccgt agcgaatggt atcgtgaagg tcgcgtgcca ctgcatactt tacgtgcaaa
     6241 tgtagattat gcaaccagcg aagcgcacac cacatatggt gtattgggtc tgaaagtttg
     6301 ggtttatacg gaaggcaata ttaaatcttc caaacctgaa catgagagta aacaaagaaa
     6361 ggcaggtaga cgtaatgctg cagccaacta gactgaaata ccgtaagcaa caaaagggtc
     6421 gcaatactgg catcgctact cgcggtaata aggtaagttt cggtgagttc ggcttgaaag
     6481 ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc
     6541 gtcatatcaa acgtggtggt cgtatttgga ttcgtgtatt ccctgataaa ccgattactg
     6601 aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat tacattgccg
     6661 aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg
     6721 aagcattcga gttggctgct gccaaattgc ctattcctac aacctttgta gtaagacagg
     6781 tgggtcaata atgaaagcaa atgaattgaa agacaaatcc gttgagcagt tgaatgcaga
     6841 tttgttggac ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt
     6901 aggcaaacca agtgaattga aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt
     6961 aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa
     7021 gtagtaagcg acaaaatgga taaaaccgta acagtattgg ttgagcgtaa agtaaaacat
     7081 ccgctgtatg gtaagattat tcggttatct actaaaatcc atgcccatga tgaaaataat
     7141 caatatggaa ttggtgatgt ggttgttata tcggaatccc gtccattgtc aaaaactaaa
     7201 tcttgggttg tcagtgagct ggttgagaaa gcacgttcta tttaagaatt aaagcaacgt
     7261 gcttggaatg ggaaacgaag tattgcagca aatttaattt gcgtgtaaac ttcgtttcct
     7321 gtctttcagt ttcttctgga agtttcttcc ctttcggggt ccaagactgg tttacttgaa
     7381 ccgcaaggtt tcatttaata agcagcggct ttgctgtaag ttatctgaaa gtggtaaatt
     7441 aagttggtta atttaaaggt aataacatga ttcaaatgca gaccatctta gatgtggctg
     7501 ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt gggcggatct aagcgtcgct
     7561 acgcttctgt tggcgatatt attaaagtgg cagttaaaga tgcggctccg cgtggccgtg
     7621 tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac tgctaagggt gtacgtcgtc
     7681 ctgatggtgc gttaattaaa ttcgataaca atgccgccgt gttactgaat aataaacttg
     7741 aacctttggg tactcgtatc tttggtccgg taacccgtga attgcgtact gagcgattta
     7801 tgaaaatcgt ttcattggca cctgaagtat tataaggaat ggcacgatga ataaaatcat
     7861 taaaggcgat agggttgtag taattgctgg taaggataaa ggtaagcagg gtcaagtagt
     7921 tcgagtgttg ggtgataaag ttgttgttga gggcgttaat gttgtaaaac gccatcaaaa
     7981 acctaatcca atgcgtggca ttgagggcgg tattattact aaagaaatgc ctttggatat
     8041 ttctaatatc gcaatcctga atccggaaac taataaagcg gaccgtgttg gtattaagct
     8101 gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc aaatcaaatg gctctatcat
     8161 tggggcataa ggagataaca tggctcggtt gagagagttt tataaagaga cagttgttcc
     8221 tgaattggtt aaacaatttg gttacaaatc agtaatggaa gtcccgcgta ttgaaaaaat
     8281 caccttgaat atgggtgtgg gtgaggctat tgctgataaa aaagttatgg aacatgctgt
     8341 ttccgattta gagaaaattg ccggtcaaaa accggttgtt actgttgccc gtaaatctat
     8401 cgcaggtttt aaaatccgtg ataactatcc ggttggttgc aaagtaacat tgcgtcgtga
     8461 tcaaatgttt gaattcttgg atcgtttgat tactattgca ttacctcgcg tacgtgactt
     8521 ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat tacaatatgg gtgttcgtga
     8581 gcaaattatt tttccggaaa ttgaatacga taaaattgat gctttgcgtg gtttgaatat
     8641 tactattact actacagcaa aaaccgatga ggaagcgaaa gctttattgt cattgtttaa
     8701 atttccgttc aaaggataat catggctaag aaagcactta ttaatcgtga tctgaaacgt
     8761 caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa ttaaagcggt aatcaatgat
     8821 tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga ggtttcaatc cattcctcgt
     8881 aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga caggtcgccc tcgtggtact
     8941 ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa tcgccatgcg tggcgaaatt
     9001 ccgggtgttg ttaaagccag ctggtaatag gagtaattaa gaatgagtat gcatgatcct
     9061 atttccgata tgttgactcg tatccgcaat gcgcaacgtg ctaataaagc agcggttgca
     9121 atgccttctt caaaattaaa gtgtgctatt gcaaaggtat tgaaagaaga aggatatatt
     9181 gaggacttcg cagtttcatc tgacgtaaag tctatattgg aaattcaatt aaaatactat
     9241 gcaggtcgtc ctgtaattga acaaatcaag cgtgtatctc gccccggttt gcgtatttat
     9301 aaagcgtcta gtgagattcc aagtgttatg aatggcttgg gtattgctat tgttagtact
     9361 tctaaaggtg taatgactga tcgtaaagca cgttctcaag gtgttggtgg tgagttgtta
     9421 tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc gcaaaaaacc cagtgactgt
     9481 tcccgctggt gtagaagtaa aatttggaac agaggcatta gttattaagg gtaagaacgg
     9541 tgaattgtct tttcctttgc attctgatgt agccattgaa tttaatgatg gcaaattggc
     9601 ttttgttgcg aataacagca gtaaacaagc aaatgcaatg tctggtactg ctcgcgcatt
     9661 agtcagcaat atggttaaag gtgtttcaga aggttttgag aaaagattgc aattgatagg
     9721 tgtgggttat cgtgctcaag cacaaggtaa aatcttgaat ctgtctttgg gtttttctca
     9781 tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa actcctagcc aaacagagat
     9841 tgttttaacc ggctcggata aacaagttgt tggtcaagtt gctgctgaga ttcgtgcgtt
     9901 ccgtgctcct gagccttata aaggtaaagg tgttcgctat gtaggcgaag tagtggtaat
     9961 gaaagaagcc aagaaaaaat aattgaggtt cactaatgga taaacataca acccgactcc
    10021 gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa aatggtaaga ttatgtgtgt
    10081 tccgaagcaa taatcatatt tatgctcaag taattagtgc tgaaggtgat aaagtattgg
    10141 ctcaagcctc tacattggaa gctgaggtgc gcggtagtct gaaatctgga agcaatgttg
    10201 aagcagctgc aatagttggt aaacgtatcg ctgagaaggc taaagcagca ggtgtagaaa
    10261 aggttgcttt tgatcgttca ggtttccaat atcacggtcg tgtgaaggct ttggctgaag
    10321 ctgctcgtga aaatggttta agcttctaaa tatttggaga ctttcagatg gcaaaacatg
    10381 aaattgaaga acgcggtgac ggtctgattg aaaagatggt cgcagttaac cgtgtaacca
    10441 aagtagttaa aggtggtcgc attatggctt tctctgcgct aactgttgtt ggtgatggag
    10501 atggtcgtat tggtatgggt aaaggtaagt caaaagaagt gcctgttgca gtccaaaagg
    10561 cgatggatca agcacgacgc tctatgatta aggtaccatt aaaaaatggt acgattcatc
    10621 atgaggttat tggtcgacat ggtgctacta aagtatttat gcagcctgct aaagagggta
    10681 gtggcgtaaa agccggtgga cctatgcgtt tggtttttga tgctatgggc attcataata
    10741 tctccgccaa agtgcacgga tctactaacc catataatat cgtacgtgca acattagatg
    10801 gtttgtctaa gttgcatact cctgctgata tcgcagccaa acgtggcttg acagtggaag
    10861 acattttggg agttaaccat ggctgaacaa aaaaagatta gggttacatt ggttaaaagc
    10921 ctgattggta caattgaatc tcatcgtgca tgtgcacgcg gtttaggttt gcgtcgtcgc
    10981 gagcatacgg tagaggtttt agatacccct gaaaaccgtg gtatgattaa taaaatcagc
    11041 tacttgttga aagtggagtc ttgatatgtt tttgaataca attcaacctg ctgttggtgc
    11101 tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt ggtcttggca aaacgggtgg
    11161 tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt cataaggtgg gtttcgaagg
    11221 tggtcaaatg cccttgcaac gacgcctccc taaaagaggt tttaaatctt taacagcatc
    11281 agctaatgca cagcttcgtt taagtgaact ggaatcaatt gctgttaatg agattgatat
    11341 tttggtctta aagcaagcgg gtctgattgc atctacagtc tctaatgtta aagttattgc
    11401 ttttggtgaa atttctaagg cagttgcttt gaagggtatt aaagttacca aaggtgcgag
    11461 agctgctatc gaggctgttg gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat
    11521 caacaaacgt catcaggttc atccaaattt ggagatctta agaaacgtct tttgtttcta
    11581 tttggagcat tgattgtttt tcgaattggt gcccatatac ccgtacctgg agttgatgct
    11641 gttgctttag ctaaattata cgaaagcgct ggaaacggca tcctgggaat attgaatatg
    11701 ttttccggtg ggtcgttaga gcgctttagt atatttgcaa taggaattat gccatatatt
    11761 tcagcttcta ttattgtaca gctcgcttct gaaattttgc catcattgaa ggctttaaaa
    11821 aaagaagggg aggctggtag aaaggtaatt acgaaatata ctaggtatgg tactgttttg
    11881 ttagcaattc ttcaaagtct aggtgttgca tctttcgtat ttcagcaagg aattgttgta
    11941 acaagttcat ttgagtttca tgtttccacg gtagtttctt tggtaacggg aactatgttt
    12001 cttatgtggc ttggggagca aattactgaa aggggtatcg ggaacggtat ttctttaatc
    12061 attacggcag gtattgcttc aggtattcct tcgggtattg caaagctggt tacactgacg
    12121 aaccaaggtt ctatgagcat gcttacggcg ttgtttattg tatttggtgc cttattatta
    12181 atttatttgg ttgtatactt tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa
    12241 cgccagttta atggtagggc gggtagtcaa aatacgcata tgcctttcaa gttgaatatg
    12301 gctggtgtta ttcccccaat ttttgcttcc agtattattc tatttccatc tactctttta
    12361 ggttggtttg gttcggctga tacaaatagt gttttgcaca aaatagctgg attgttacaa
    12421 cacggtcaat tgctgtatat ggctttattt gcagcgacag ttattttctt ttgttatttt
    12481 tatacggctt tggtttttag ccctaaagaa atggcagaga atttaaaaaa gagtggtgct
    12541 tttgttcctg ggattagacc tggtgagcag acctctaggt atttagaaaa agttgtatta
    12601 cgtttgacat tgtttggagc tctttatatt acaactattt gtttaattcc agagttctta
    12661 actacggttt taaatgtacc tttttatttg ggtggcacgt ctttgttgat tctagttgtt
    12721 gtaacgatgg attttagtac acaaataaat tcgtataggc ttactcaaca gtatgataag
    12781 ttaatgactc gttcagaaat gaaatcattt tctcggaaat agaattatgg cgaaagaaga
    12841 tactatccaa atgcaaggtg aaattcttga aactttacct aatgcaacat ttaaagtaaa
    12901 acttgagaat gaccatattg tattgggtca tatttctggg aagatgcgga tgcattacat
    12961 tcgtatttct ccgggagata aggtcacagt agagctgaca ccttatgatc taactagggc
    13021 tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca
    13081 tctgttaaga aaatttgccg aaattgcaag attattcgtc gaaatcgtgt agttcgtgta
    13141 atttgtactg atctccgtca caaacagcgt caaggttaat ggaatatttc ttttaatgtg
    13201 attctgtgat atagtgacac actttgccct aaaaaggaaa aaatatggct cgtattgcag
    13261 gggtaaatat ccctaataac gcacacatcg taattggtct tcaggctatt tacggtattg
    13321 gtgctactcg tgctaaattg atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa
    13381 aagatttgga cgagactcaa ttagatgctt tgcgtgacca agttgccaag tatgaagtag
    13441 aaggtgattt gcgtcgtgag gtaactatga gtatcaagcg attgatggac atgggctgct
    13501 atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg tcaacgcact cgtacaaatg
    13561 cgcgtacccg caaaggtccg cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt
    13621 ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg
    13681 agtgagggta ttgtgcacgt tcatgcatct ttcaacaata ccatcattac aatcactgac
    13741 cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg ctggttttaa aggttctcgt
    13801 aaaagtacac catttgcagc acaagttgca gcagaagcag ctggtaaagt tgcccaagag
    13861 tatggcgtta aaaatttaga ggttcgtatt aaaggtccag gtccaggtcg cgaatcctct
    13921 gtacgtgctt tgaatgctct tggtttcaag attaccagca ttactgacgt taccccgttg
    13981 cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa
    14041 catggcacgt tatattggcc ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt
    14101 tttgaagagt gcgcgccgct ctttggattc taaatgtaaa attgattccg ctcctggtca
    14161 gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa
    14221 aatccgccgt atttatggcg tattagaacg tcagttccgt cgttatttcg cagaagctga
    14281 tcgtcgtaaa ggttctaccg gcgagttgct gttgcagttg ctggaatctc gtttggataa
    14341 tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa gcaagacagc ttgtttctca
    14401 taaggcgata gttgtgaatg gacaagttgt caatattcct tctttccaag tgaaagctgg
    14461 tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta cgtattcaag aagcattggg
    14521 tttggcaact caaatcggct tgccgggttg ggtttctgta gatgcggata aacttgaggg
    14581 tgtgttcaaa aacatgccgg atcgctcgga attgaccggt gatattaatg aacagctggt
    14641 ggtagagttc tactctaaat aatgctagct cagtgaggga cagttaaatg cagaatagca
    14701 caaccgaatt tttgaaacct cgtcaaattg atgtaaatac tttttctgca actcgtgcaa
    14761 aagtatctat gcagccattt gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc
    14821 gtatcttact gtcatccatg aatggttttg ctcctactga agtagctatt gccggtgtat
    14881 tacacgaata ttctactgtt gatggtattc aggaagatgt tgttgacatt ttgctgaata
    14941 ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat
    15001 caggttcagg tgtcgtatct gccggtgata ttgagttgcc gcatgatgta gaaattctga
    15061 atcctggtca tgtcatttgt catttggctg ataacggtca aattgagatg gaaattaaag
    15121 tagagcaagg tcgtggttat caatctgttt caggtcgtca ggtagttcgt gatgagaacc
    15181 gtcagattgg tgcaatccag ttggatgcga gcttttcgcc catcagccgt gttagctttg
    15241 aggttgaacc tgcacgtgta gagcagcgga cggatcttga taagttggtt ttggatatcg
    15301 aaaccgacgg ttctattgat cctgaggaag ctgtacgcag tgcggcacgt attttgattg
    15361 atcagatgtc tatttttgct gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag
    15421 cacctcctat cgaccctgtt cttttgcgtc cggtggatga tctggaattg acagtacgtt
    15481 cagctaattg tttgaaagct gaggatattt attatattgg cgatttgatt caacgcactg
    15541 aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc tttgaatgag attaaggaag
    15601 tattggcatc taaaggtttg acactgggtt ctaagttgga agcatggcca cctgtaggct
    15661 tggaaaagcc ttaatgaaga attaaaggat aattgatatg cgtcatcgta atggcaatcg
    15721 caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt
    15781 attgactcac gaaactattg taacaactct gcctaaggcc aaggaattgc gccgtgtagt
    15841 agagccgttg attacattgg gtaaaaagcc gtcattggca aaccgccgtt tggcatttga
    15901 ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc gatttgggtc ctcgttttac
    15961 tgctcgtaac ggtggttatg ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc
    16021 acctctggca ctggttgaat tggttgacaa accggctgct gagtaatttt agtcatataa
    16081 cgccatctgc cgaaaagcag gtggcgttat ttttgcaata tctgataggt aatagggtat
    16141 tggctatcat gtttaaaata ttaattgaat agctaaggtt tgcgcggtaa acttacatca
    16201 ttaaaaaatt ctatgatggt ttatataatg aatgctttcg atataaagtc gacaaagatg
    16261 gacgtattgt ctatatcttt gcatacgtca gacttgtttg atttggaaga tgtgctggtc
    16321 aaattgggca agaagtttca agagtctggt gttgttccat ttgtgctgga tgttcaagag
    16381 tttgattatc ccgagtcttt ggatcttgct gcattggttt cgttgttttc aaggcatggt
    16441 atgcaaattt tgggtctgaa gcattctaat gaacgttggg ctgctgcggc tatgaagtat
    16501 catttgctgt tttgtctgtc tcattcggaa aatgttaaag aactgggtca ggttgaggtg
    16561 cagaacacgg aggatggtca gaaagcaagg aaaacagtat tgattacatc ccctgtccgt
    16621 accggtcagc aggtttatgc cgaagatggc gatttgattg ttacgggggc ggtcagccag
    16681 ggggcggaat tgattgcgga tggcaatata catatttatg cgccgatgag ggggcgtgct
    16741 ttggccggtg ccaagggtga tacttctgcc cgcatattta tccactccat gcaggcagaa
    16801 ctggtttctg tggcgggtat ttaccgtaat tttgaacagg atttgccgaa ccatctgcac
    16861 aagcagccgg tacagatatt gttgcaggat aaccgattgg ttatcagtgc aattggctca
    16921 gagtaattgt ttgatatttt aaaaaggaaa tattgtggca aaaattattg tagtaacttc
    16981 aggtaagggc ggtgtcggta aaacgactac cagtgccagt attgcgacag gtttggcatt
    17041 acgcggatat aaaactgcgg taattgattt tgatgtgggt ttgcgtaacc tcgacctcat
    17101 tatgggttgc gagcgtcgtg tcgtttatga cctgatcaat gtcattcagg gggaggcgac
    17161 gctcaaccag gctttgatta aagataaaaa ttgtgaaaac ctgtttattt tgccggcttc
    17221 ccagactcgg gataaagacg ctttgacacg cgagggcgta gaaaaagtga tgcaggagct
    17281 gtccggcaag aaaatgggct ttgagtatat tatttgcgac tctcctgccg gtattgagca
    17341 gggtgcattg atggcgttgt attttgctga tgaagccatt gtaacgacca atcctgaggt
    17401 ttccagtgtg cgtgactccg acaggatttt gggaattttg caaagcaaat cccataaggc
    17461 agagcaaggc ggttcggtta aagaacatct gttgattacg cgttattctc ccgaacgtgt
    17521 ggcaaaaggc gaaatgctgt ctgtacagga tatttgcgat attctgcata ttcctttgct
    17581 gggtgtgatt cctgaatccc aaaacgtctt gcaggcatcc aattccggag aaccggtcat
    17641 ccatcaggac agcgtggcgg cttccgaggc atataaggac gttattgccc gtcttttggg
    17701 cgagaaccgt gaaatgcgtt tcttggaagc tgagaaaaaa agcttcttca aacgtctgtt
    17761 tggaggataa ggtatgtcat taatcgaatt tttattcggc agaaagcaga aaacggcaac
    17821 cgttgcccgc gaccgccttc aaatcatcat tgcccaagag cgcgcccaag aaggtcaggc
    17881 tccggattac ctgccgactt tacgtaaaga gttgatggaa gtcctgtcca aatatgtgaa
    17941 tgtttcatta gacaatatcc gtatttccca ggaaaaacag gatggtatgg atgtgcttga
    18001 gttgaacatt actttgccgg aacagaaaaa ggtataggac atgaccttaa ccgaattgcg
    18061 gtacatcgtc gcagtcgccc aagaacgtca tttcggcagg gcggcgcggc gttgttttgt
    18121 cagtcagccc actttgtcta ttgccattaa gaaattggaa gaagagcttg ccgtctcttt
    18181 gtttgaccgg agcagcaacg atattattac gaccgaggcg ggggaacgta tcgttgcaca
    18241 ggcgcgtaag gtgttggaag aggcagagct tatcaggcat ttggcaaatg aagaacaaaa
    18301 cgagctggag ggtgcgttca aactcgggct gatttttacg gttgcgccgt acctgctgcc
    18361 gaaactgatt gtctcgttgc gccgtactgc accgaaaatg cctttgatgt tggaagagaa
    18421 ttacacgcat actttgaccg agtcgctcaa acgcggggac gttgatgcga ttatcgttgc
    18481 cgaaccgttt caagagccgg gcattgttac cgaacccttg tatgacgaac cgtttttcgt
    18541 gattgtcccg aaagggcatt catttgagga actggatgcc gtttcgcccc ggatgctggg
    18601 tgaggagcag gttttgctgc tgacggaagg caactgtatg cgggatcagg tactctcaag
    18661 ctgttccgaa ttggcggcga aacaacgtat acaggggttg accaatacat tgcagggcag
    18721 ctcgattaat acaatccgcc atatggttgc cagcggtttg gcaatcagcg tgttgccggc
    18781 aaccgcactg accgaaaacg atcatatgct gttcagcatt attccgtttg agggtacgcc
    18841 gccaagccgg cgggtcgtat tggcgtaccg ccgcaatttt gtccgtccga aggcgttgtc
    18901 ggcgatgaag gcggcgatta tgcagtcgca gcttcacggg gtaagtttta tccgcgacta
    18961 ggcgcaggca ttgttttcaa aacgccattt ccctgagccg acaacacggt atgccaagat
    19021 attgccgtca tcatcgattt tgagtatagc atcgccacgg aaactgccgt cctgaagata
    19081 ttcgactttt gcatcactgt gaatgttttc atcagtgccg atgcaatgcc atgtatagtg
    19141 gattaacaaa aaccagtacg gcgttgcctc accttgccgt actatttgta ctgtctgcgg
    19201 cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa agaggccgtc tgaaaaacat
    19261 ttttcagacg gccttgttga ttcaatcaaa tcagtctttc aacttcgaca actgagtttg
    19321 cacttttgcc attttgtctt ccaattccgc caaatcggct ttgtcttttt ccaccaaatg
    19381 cgccggggct ttttcggtgt agccgggttt ggagagtttg gcgttgagtt tgtccaaggc
    19441 tttttgcagc ttctcggctt ctttgctcaa gcgggcggtt tcggcagctt tgtcgatttc
    19501 gactttcaac atcaggcgcg cgccgttgca gacggcgacg ggcgcgtctt cgctttcggg
    19561 tagggcggcg acttgctgtg cttcggtcag gcgggtcatc atcggcaggt atttgaggta
    19621 gtccgccaag tcgtctgcgc tttcgacaaa cagcggggct ttcacgttgg gctggatgcc
    19681 catttcgccg cgcaggttgc ggactgcgcc gatcaaatcc tgcaacacgg tcatttgccc
    19741 gaatgccgtc tgaacaatct cgccgctgtc ggcttcgggg aagcgggcga gcatgatgct
    19801 gtcggcggtt ttcgcgtcgc acataggagc gacggtttgc cacagttctt cggtgatgaa
    19861 cgggataatc gggtgcagca ggcgcagggc ggcttcgagt acgcgcaata aggtatggag
    19921 tgtggcgcgt tggcggctgg cgcagccggt ttgaagctgc actttggcaa gttctaaata
    19981 ccagtcgcaa tagtcgttcc acatgaagct gtacagggtt tccgctgcca aatcaaagcg
    20041 gtaggtttcg taggcttgcg ttacttgctc gatggtctga ttcagacggt ctacaatcca
    20101 catatcgggg aaggaatagc cgcgcggctc agtggcggtt gcgccgtagc cgcagtcttg
    20161 gttttcggtg ttcatcaaga cgaagttggt ggcgttccag attttgttgc agaagttgcg
    20221 gtagccttcg gcgcgtttga agtcgaagtt cacgctgcgc cccaagctgg cgtagctcgc
    20281 catggtgaaa cgcaatgcgt ccgcgcccat actcggaatg ccttcgggaa agagtttttt
    20341 ggtggcttct tccactttcg gcgcggtttc aggtttgcgc aggccggtgg tacgttttac
    20401 cagcagcttg tccaagccga tgccgtcaat caaatccaca ggatcgatga cgttgccttc
    20461 ggatttggac atttttttgc cttcgtggtc gcgcacgatg ccgtggatgt acacgtcttt
    20521 aaacggtact ttgccagtaa agtgggtggt catcataatc atgcgcgcca cccagaagaa
    20581 gatgatttcg tagccggtta ccaagacgtt ggacggcagg aaggctttga gttcgtcggt
    20641 ttcagacggc cagccgagcg tggaaaacgg cacgagcgcg gaggagaacc aagtgtccaa
    20701 tacgtcttct tcgcgggtca agcctgtttt gccggcttgt ttttcggctt cagcctgatt
    20761 gcgggcgacg tacacattgc ctgtttcgtc gtaccacgcg gggatttgat gcccccacca
    20821 cagttggcgc gaaatacacc agtcttggat gttgttcatc cattggttgt aggtattgac
    20881 ccagttttca gggataaagc gtaccgcgcc gctgtcgacg gcttttttgg ctttgtcagc
    20941 aaggcttaag cctttgaatt cgctgtcagg ctcgccgccg tttggcgtgg cggacatggc
    21001 gacaaaccat tggctggtca gcattggctc aatcactgag ccggttcggt cgcctttggg
    21061 tgtcatcagc gtgtgtgctt tgatttcgac caacaagcct tgttcctgca aatcggcaac
    21121 catttgtttg cgcgcggcaa agcggtctaa acctgcgtat ttttcaggca gggcaaagcc
    21181 ttgttgcgct tcgcctttga agttgaacac ttcggcgttt gccagcactt tggcttccaa
    21241 gtcgaacaca ttaatcaggc gcgtgtcgtg gcgtttgccg acttcgtagt cgttgaagtc
    21301 gtgcgcgggc gtgattttca cgcagccggt gccgaagtct ttttcgacgt attcgtcggc
    21361 aatcacggga atggtgcggc cggtcagcgg caggattaat tccttgccaa tcaaatgggt
    21421 gtaacgttcg tcttcaggat tgacggctac ggcaacgtcg cccagcagcg tttcgggacg
    21481 ggtggtcgcc acgatgacgg cttcggaggg attgtccgcc agcggatagc ggatgtgcca
    21541 catagagcct tgttcttcca cgctttccac ttccaaatcc gataccgccg tgccaagaac
    21601 gggatcccag ttcaccaggc gtttgccgcg ataaatcaag ccctgttcat acaggcgtac
    21661 gaacacttct gtcacggttt cggcgcgcac gtcgtccatc gtgaaatact cgcgcgtcca
    21721 gtcggcagag cagcccacgc ggcgcatctg ctgcgtaatc gtaccgccgg aaacttcttt
    21781 ccattcccac actttttcca agaatttttc gcggcccaag tcatgacggg acacgttttg
    21841 cgcggcaagc tgacgctcga ccacgatttg cgtggcgatg cccgcgtggt cggtgccggg
    21901 aatccagacg gtgttgcagc ctttcatgcg gtagtaacgg gtcagaccgt ccataatggt
    21961 ttggttgaag gcatgaccca tgtgcagcgt gccggttaca ttgggcggcg gcagttggat
    22021 ggaaaaagac ggtttggtca aatccatatc aggttggaaa tagccttggc tttcccagtt
    22081 ttgatagtgc ttggattcga tttcggctgg attgtatttg tctaacatga tggaactttg
    22141 tgaaattaag gttatttttg atgtgcggat tataacgcaa aaaggccgtc tgaatcattt
    22201 cagacggcct ttggcataca ggttttaaaa atggaacaat accaggctga cggcaatcac
    22261 cgccatgccc attgtcaggc cgtaaacggt ttcatggccg tctgaatagc gtttggcagc
    22321 cggcagcagc tcgtccaacg ccaaaaacac catcacaccg gctatcacgc cgaataccga
    22381 accaaacacg gcaggcgaca aaaacggctg caaaaccaaa tagcccaaag ccgcccccaa
    22441 cggctcggcc aagccggata gcagacacgc ccacaccgtt ttcttacggc tgcgggtggc
    22501 aaaataaacc ggcgcggcga tggaaatgcc ctccggaata ttatggatgg caatcgccaa
    22561 ggccaaaggc atcccgactg ctggattttc caatgtggca aaaaacgtcg ccaagccttc
    22621 ggggaaattg tgcgcagtaa tcgcaaacgc cgccatcatg ccgactcgcg cgatatggcg
    22681 gcgtttgctt tcttgaaacg acgggtcttg cgcgtctaaa gtttcatgcg ggttcggcac
    22741 cagacggtca atcagcgcaa tgccgcccat ccctgccaaa aatgccatgg tcgccgccgc
    22801 aaacgcgtgg tctttatcat aaatttcagc gaacgcctcg ctggacttac tgaaaatctc
    22861 cgtcagggaa acatacacca tcgcaccgcc ggcaaatgcc aaaccaaacg acaacacgcg
    22921 cggattgggc gttttggaaa acatcaccaa gccgctgcct aatacggtaa acaaaccggc
    22981 agccaacgta atggaaaagg caacggccaa attggacatc gaaaaatcgg gcatgagaaa
    23041 acctgcgcta aaagctggga caggttcaga ctaacacttt ttaatgtata tgataatagt
    23101 tattatttat tttattgatt ggatacacgg attttgaaac aaaaggccgt ctgaaaaatg
    23161 attttcagac ggcctttaaa tttgaaatgc cgctaaacct tagtgctttc cagcttaagc
    23221 ctgataacgc gacaggctca aatcgtcgct gcggatttcg gtgtctttgc cgctcacgat
    23281 atcggcggtt aattttgccg aacccagcga catggtccag cctaaagtac cgtggccggt
    23341 attcagaaac aggttgtcaa agcgggtgcg accgattaac ggcgtgctgt cgggcgtcat
    23401 cggcctgagg ccgctccaga acgatgcttg gctcaaatcg ccgccttccg ggaacaagtc
    23461 gttgacgacc aaagccaagg tttcgcggcg tttttcgggc agtttgattt cgtagcccga
    23521 caattccgcc ataccgccga cgcggattct gttgtcaaag cgcgtgatgg cgactttgta
    23581 gctttcatct aaaacggtgg acaccggcgc gccgtcggaa tttgtgaccg gcagggtcaa
    23641 ggaatagcct ttgacgggat aaatgggcag attgagatcc aactgcgcca aaaccgtcct
    23701 gctgaaacaa ccgagcgcgc agacaacggc atctgcttca aaccgccctg tttcggtttc
    23761 aacggttttg atgcgcagcc cgttgtggtc gatgcggctg atggtttggt tgaaatgaaa
    23821 ccgtacgccc ttttcctgac acaatttgta taggttttca gtgaagaggc ggcagtcgcc
    23881 ggttgcatcc gcaggcaggt gcaggccgcc ggcaattttg gcggtaacgc gtgccagcgc
    23941 aggctcgaat tctgcgcatt cttcgggctt cagacggcgg tacggcacgc cgtagcgttc
    24001 caaaacggca atgtcttgtt ctgcagcttc aacttcttta gcctgacgga aaatctgcaa
    24061 cgtccctttt ttgcgccctt caaaattcat gccggtttgc gcttcaaaac ggcggaacat
    24121 ttcacggctg tattcggaaa tcctgaccat gcgctcttta ttggtttgat agtgcgctgc
    24181 cgtgcagttt tgcagcatcc gccacagcca ttcgatttga tacaggctgc cgtcggggcg
    24241 aaacagcaaa ggcggatggc ttttaaacag ccatttcagt gctttggtcg gaataccggg
    24301 tgcagcccaa ggcgtggtat agccgtaaga aagctggcct gcattggcaa aactggtttc
    24361 catcgccaca cccttggcgc ggtcgatgac cgttacttca tgtccggcct ctgccagata
    24421 ccacgcggaa gacacgccgg caacacccgc acctaaaaca agcactttca tgtttctccc
    24481 tccgactttt tcaaaacaga cttaatatgc cgtgccgtct gaatattcgg attcagacgg
    24541 cctcggatat taatgcggca attcgccgtt tgtgattttt tgtttgaagt cgcgcgtttc
    24601 attgacgatg actttcgcca tcaataaaag tgcaatcagg ttgggcaatg ccatcaagcc
    24661 gttgaatgtg tccgaagcca gccacaccaa atcaaggctc aacacggtac ccagcataac
    24721 ggaagaaaca taacccacgc ggtacaaacc ggcaaatttc tcgccgaaaa catacaccgc
    24781 gcatttttcg ccgtaatagc accagcccaa aatggttgag taggcaaaga aaatcaggcc
    24841 gatggtaaca atccagccgc cgatgccggg cagcattttt tggaatgtga cggttgtcag
    24901 tgccgcgccg ctcacttcag gtttgacaaa ctcgccgccc gcgccgagca gtcccattac
    24961 caacacgatg ccggtaatcg agcaaacgac gatggtatcc aaaaacgtac cggtcataga
    25021 aaccaaggcc tgacggacgg gatggtcggt tttcgcggct gcggcggcaa taggcgcaga
    25081 acccataccc gcctcattgg agaacacgcc gcgcgccacg ccgtagcgga tgaccgtacc
    25141 gatagcgccg cccgccactg cctgcgcgct gaacgcatcg gagaaaatca gcttgacggc
    25201 aggcatcagt gcatcggaat taatcgcgat aatggaaaga ccgcccaaca cataaaacac
    25261 cgccatagca ggcacgatga aagaagcggc tttggcgatg cctttaatac cacctaaaac
    25321 gacaacggca gtcagaacgg tcaacgtaat gccggtatag gcaggttcga taccgaagct
    25381 ggtttgcacc gcctgtgcaa ccgagttgga ctgcaccgag ctgccgatac cgaaggaagc
    25441 gaatgtgccg aacagcgcaa atgcaacggc catccatttc cagtttttgc ccaagccttt
    25501 ttcgatgtaa tacatcgggc cgccggacat ttcgcctttg gaattgttga cgcggtattt
    25561 caccgccaac acgccttcgc cgtatttggt ggccatgccg aaaatggcgg tcatccacat
    25621 ccaaaatacc gcgcccgggc cgccggttac caccgcagtc gccacgccgg cgatgttacc
    25681 cgtaccgatg gtggcggaca gcgcggtcat caacgccgca aaatgggaaa tatcgccttc
    25741 gtggccttcg ccgccttcat acttctttga cggcacaaac gcctgtttca gcgcatagcc
    25801 caacatagtg aactgtaagc ctttcagcat aacggtcagc acaatgccgg tgccgaccag
    25861 cagcatcaac ataacagatc cccagaccca gccgctgacg gtttcgaaaa aggctttgag
    25921 attgtctaaa aacaattgca tggctttctc ctttgtctgt tttattttta aaacaccact
    25981 tttgtagtgt cctgtaattt cagcacagaa tattcaataa gacaatatgt tcttttgaaa
    26041 aatacttttg gttttttctc cgaaagcagg tcggttcaag ctgcggaaat tgtttgcaat
    26101 tctttaaaag cagcggcgga ggtcacaatg aaatgtccga atggggatgt ggcgggcggc
    26161 agaaatcatc aatgctgccg actgccatac ttctgaaatc tacaaaatga tgcatcgatc
    26221 aaacaatata ccgctttaaa aaaaccgatg ccgtctgaaa cgctttcggg gtttcagacg
    26281 gcatcaaaag ggtacggtca gcggatgatg ccgcgcgccg attgtgcgaa aaagtctcgg
    26341 aatacggcaa gctcggcttg ggtttcggcg cggcggagga tgtccgcctt ggcctcttca
    26401 aacggaatgc cgcgatggta gagggttttg tacacgtctt tgacggcgga aatctgctct
    26461 gcggtaaaac cgttgcggcg cataccttcg ctgttgaggc ccgccggttc ggcgcggtag
    26521 cccgatgcca taaagtaggg cggtacgtct ttgtgtacgc ctgcggcgaa ggcggtcatg
    26581 gcgtagtcgc cgatgcggca gaattggaaa accagcgtgt agccgcccaa aacgacgtag
    26641 tcgccgatgg taacgtgtcc ggcaagcgag gcgttgttgg cgaagatggt gtggttgccg
    26701 accacgcagt cgtgcgcgag gtggcagtac gccataatcc agttgtcgtc gccgatacgg
    26761 gtttcgccga tgccggttac cgtgcctaaa ttaaaggtgg tgaattcgcg gatggtgttg
    26821 ccgttgccga taatcagctt ggtcggctcg tcgcggtatt ttttgtcctg cgggatttcg
    26881 ccgaggctgg caaattggaa aatgcggttg ttttcgccga tgctggtgtg gccgttgatg
    26941 acggcgtgcg gaccgatttc ggtgttcgcg ccgatttgga cgttggggcc gataacagtg
    27001 tacgcgccga ctttgacgct tgagtcgagt tcggctttgg ggtcgatgac ggcggtcggg
    27061 tggatgaggg tcatgttttt aatactccgc tgcttgttaa gacaaataat agaaaatcac
    27121 agtttgtcga tttcatcgca gaccgcaatc acttgatcgc taaaatggct aaatactgaa
    27181 gcagtatcta catccatgta attaagcagt tcaaaaatag attggtggat atttctagcg
    27241 tagccggtat cttttattgc acctttaaaa caaatcggct cgtgatgtgc caaacgatta
    27301 cggaaattat taatattgga aagttctcga aaaatccatt tttggttatg ttgcacactt
    27361 ggtgtagatt tgggcttttt tggaaaaact ttcatcaata ctttccctgt agcatcaaat
    27421 tgggcatctt tccctcccgc aaacagatac cgccaaaaac cgaatcctag tcctgcgacc
    27481 aattggttgt ggtcaaactt tcctccgcta ttattttgta ttttagataa tgcaaccttg
    27541 attaattcag cagattcttt cgtaccattt cttaaaaagc aaccttgata tttcaatgca
    27601 ggattcgttt gtggctgtat gctgttatat aaccagtttc cgtctttaaa cgcctgctga
    27661 aggcaaatgt caattttgtt tcttaaaaca atctcaaata ttgaaacaac atgaaaaagt
    27721 tctcttgata aagataaatt caataaatac aattcagctg ctttgtcttt cccaacggca
    27781 tttttatacc gttccattct gaaatcagaa aaaatactac agcattcctt ataattcacg
    27841 ctaatacctg attccttatt gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    27901 cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
    27961 cactataaaa ttgacgtttc aaactatatt tgatacagtt aggtgaaact aatccccacc
    28021 gcgtccctgg gtttgaataa acctaaaccg gtgggttttc tttttattag gtcgtctgaa
    28081 actttcagac gacctttttc cgaacgctca aaccacgcgt ttggcgcaca taatcacggc
    28141 ttcgacggcg acttgcccgt ccactttggc aacggcgttg aatttgccga tgccgcgccg
    28201 gctggtcagc agctcgactt caaagacgag ttggtcgccg gggatgactt ggcgtttgaa
    28261 acgggcttcg tctatgccgg cgaagaagaa gaattcgttt tccttgcgcc cgccttcgct
    28321 gaggatggcg agtgtgccgc aggcttgcgc catggcttcg atgatgagta cgccgggcat
    28381 cacgggcagg tcgggaaagt ggccttggaa ctggggttcg tttatggtta cgtttttgat
    28441 ggcggttagg gttttcatcg gctcgaaggc ggtgatgcgg tcgagctgga gaaacgggta
    28501 gcggtggggg atgagttttt ggatgtcttt ggcttcgatg gggagttgaa cgtccatgtc
    28561 tgtcgtattc cttgaataaa gtcggtttgg atttattgcg tgtctttgcc gtctgaaagc
    28621 tgctgttcca gcgttttgag gcgtttgttc atttcgctta agcggtggat gtaaacggcg
    28681 ttgcgcgccc attctttatg ggtggacatc gggaagatgc cggcgaggtg tttgccgctt
    28741 tcggtaatgc tgtgggtgac ggacgtgccg ccgccgatgg tggttttgtc ggcgatttcg
    28801 atgtgtccga ccgtgccgac gccgccgccg atgatgcagt agctgcctat ggtgacgctg
    28861 cctgagatgc cggttttggc ggcgatgacg gtgtgcgagc cgattttgca gttgtgtccg
    28921 atttggactt ggttgtcgat tttggtgccg ttgccgacgg tggtgtcgct catcgcgccc
    28981 cggtcgatgt tggtgttcga gccgatttcc acgtcgtcgc ccagcgttac cgcgccagtt
    29041 tgcgggattt taaaccacga atcgccggcg aaggcgagtc cgaatccgtc cgcgccgatg
    29101 accgcgccgc tgtggatttc aacgcgtctg cccagtgtgc agccgtaata aacgacggcg
    29161 ttaggatgta aaacaacttc atcacctaaa cggcaatcgt gttggacgac ggcgtttgcc
    29221 aagatgcggc agccttcgcc gagcacggtg tttgcgccga tgtaggcgtt cgcgccgatt
    29281 tcgcagcttg cgggaacggt cgcgcccggt tcgacgacgg cggtcggatg gatgccgccg
    29341 cgcgctttga cgacgggtga aaataggcgc gcgactttgg cgaaatagag ataggggtcg
    29401 tcggcgacaa tcaggttgcg cccttcaaat ccgtctgccg ctttggcgga aacgatgacc
    29461 gcacccgcgc tgctgtcgtg gacttcggct ttgtatttcg ggttggcgag gaagctgatg
    29521 tgttccgcct gcgcgtctgc gagcgggcgc acggcggtaa cagaagtgtc ttcgccgcgc
    29581 cattcgccgc cgagccgcgc ggtgatttgg gacagggtgt aggtggccgg aatcatggtt
    29641 ttcctgttcg gtatgccgtc tgaaagggtc agcgggcgtt catttcttta atgacgctgt
    29701 cggtaacgtc gtattgggtg ttgacgtaaa tcacgtcctg caaaatgaca tcgtaacctt
    29761 cctgtttggc gattttgacg atgacgcggt tggcgttttg ctggagggag gcaaactctt
    29821 cgttgcggcg gaggttgtag tcttcttcaa actgcgcctg ttttttgcgg aacgctgcga
    29881 ccagcccgca ccatttttct tcggcttgcg ccttttttgc gtctttgagt ttgccttcgg
    29941 caagctgcct ttccaaatcc agaccttcgc gttgcagttt ttgcaattcg tcctggcggg
    30001 cggaaaattc gctgtccagc gttttttgaa tcttgcgcgc ctgcttggat tcgaggtaga
    30061 tgcgctcggt gttgataaag ccgatttttt ggaaggtgtc ggcgtgcgcg cctgcggtgc
    30121 agcacaaacc gatcagagcc gcggcaaacg cgcgggtcaa acgggtcatg gtaaaactcc
    30181 ttcgaatgtt gccgcgaaat gccgtctgaa gggcttcaga cggcatttgc gggattagaa
    30241 cgtcgtgccg agttggaatt ggaagcgttg gatttcgtct tccggttttt tcttcagcgg
    30301 gtaggcgtag ctgaatttca tcgggcctaa aggcgagagc caggtaaccg cgccgccggc
    30361 ggaatagcgc aattcgttgg taaaggtgga tttatgggta ttgccggcgc cgtaaatgtt
    30421 ttgaaccctg ccgccggtcg cggaactgct gttgtcgtcg taggttttgc cgtcccacac
    30481 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgc gcgtctttcg cgccgggcat
    30541 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    30601 gccgtattcg tcatacactt tcggaccgag cgtgccgctt tcgtatccgc gcaccgaacc
    30661 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    30721 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    30781 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    30841 gttcacgccc gtcaggtagc cgcgcgtcgg ccataacgcg ctgtcggttt tgttgcgccc
    30901 ccagccgacg gtacctttgt acagccagcc tttgaagctg ccgtctgtgc cgtcggtttt
    30961 gccgtatttc ttgataaagt cggcatagtg tttgggcgct ttgttgtagg tgttgacggt
    31021 caggtgttct gccaccaaac cgaaattcac gcggtcgtat tcggtaacag gcacgctcat
    31081 gcggatgcct gcgcctgccg tggtggtttt atattgtttg atgctggtcg atgctttgcg
    31141 cgggtcgaag gcttttccgt aaacatcgta gcccaggctg accccgtctg ccgtgaagta
    31201 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgtgagg cgcgcagggc
    31261 ggccgacttg cccgtaccga acaggttgtc ttgggaaacg cctgcggaca tgaccaggcc
    31321 ggtatcctgt acccagcccg cgctcaagtc gagcgagccg gtggaacgtt cggtcaggct
    31381 catgttcaaa tcgactttgt cgggtgtgcc ggcaagcggg acggcatcaa actgtacgtt
    31441 gtcgaagtag cccaaaagct cgacgcgctc tttggagcgt tgcagcttgg aggtgtcgta
    31501 aggcgcggat tccatttggc gcaattcgcg gcgcacgact tcgtcgcggg ttttgttgtt
    31561 gccggtgatg tggatttcgt tgacgtagat tttccggccc ggttcgatgt gcaggacgaa
    31621 atcgacggtt ttggtttcgg cgtttggcag cggctgtacg ctgatttcgc tgtatgcgta
    31681 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    31741 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggctttgg ggacttcgtt
    31801 ggtgtcgcct tcgatggaga ctttgcccca acggaaacgt ccgccttcgt ggacggtgat
    31861 tttgatggtc tgcttggttt tgtcttcgtt ggtttggatg tcggtatcga ggatgcggaa
    31921 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcaaattt
    31981 ctgctcgttg aattggttgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    32041 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    32101 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    32161 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    32221 caggccggcg actgcctgat tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    32281 gaacgattcg aggtttttct taatggcgtc gttttgcagc atttttgcgc cggtgatgtt
    32341 gagcgagccg atggtggggc gttcgatgac ggtcagcagg agctgcccgt ccgcagtctc
    32401 gacgcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    32461 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tactcggctc
    32521 ggtacgctgc aagccttcga cgcggatgtc ttggatggtg aagtcggcaa gtgccaaagg
    32581 cgatatgccc aagaccatca gtgcggaagc aatctgtttc agtttcattg tcagttcctt
    32641 gtggtgcgga atgcggtttc agacggcatt ccgaaacgta aaatctaacc gagcagccgg
    32701 gtaacgtcgt tgaagaaggc gaccgccatc atcagcatca tgagggcaag cccgaagcgc
    32761 aaaccgatgt tttggacgcg ttcgcccaaa ggtttgccgc gtatccattc ggcagtataa
    32821 aacacgaggt ggccgccgtc caaaacgggg acgggcagta ggttcagcac gccgaggctg
    32881 atgctgacca gtgccaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    32941 gcaatgtcgg caatggtcag cggcccggaa atatggctga cggaggcgtt gccgctgatt
    33001 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    33061 ccgaatgcgc ggataacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    33121 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcggactg ttcgacagta
    33181 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtagttcag ggtgattttt
    33241 ttgccggggc tttggcgggt caggtttgcc cattcttgcc atgaggcgat gggtttgccg
    33301 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    33361 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    33421 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    33481 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    33541 gtttgcgcgc tgccccaatc tgcaacgggt gtgccgttga cggattgtat tttgtcgccg
    33601 ctttggaagc cggcgcgggc ggcaatggtg tcaggttcga ctgtgccgac gtaggggcgc
    33661 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    33721 gtcagtgggc cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    33781 tagggtaaat cggcttctga tacttcgcct tcgcgcgtgt cgaccatttt gacgtaaccg
    33841 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    33901 ttgccgaagc cgacggaaaa gcgtacgact ttgacaccgc acaatctggc aacgatgtag
    33961 tgtccgaact cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtg
    34021 tgcaaatggt tttcctttga taacggtgtt cggacggcat cagcgcagtg tgccgataaa
    34081 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaag agccccccta tgtcacctat
    34141 gccgtctgaa aagtcttgtg aaagacaatg ggcgacggtt ttggcaatgt cggtaaactt
    34201 aatctgtccg tccaaaaagg cggcgacggc ggcttcgttg gcggcgttca atacgcaggg
    34261 cgcgaccccg cccgcgttca tggcttcata ggcgagcttc aggcagggga agcggtcaaa
    34321 gtcgggcttt tggaaggtca gcgcggacaa tgcgtcgaaa tcaaggtcgc cgacacccga
    34381 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    34441 cagttgtgcc agcacggagc cgtcgcggta gcgcaccatg ctgtgtatca cggattgcgg
    34501 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    34561 ctccaaacct ttgttcatca tggtggcgga atcgacggag attttgcgcc ccatacgcca
    34621 attggggtgt ttgaccgctt gggcgggcgt aatgcggtcg aacgtgccta aatcggtcgt
    34681 taaaaacggg ccgccggaag cggtcaggat aatcgaacgg atgccgtgtt cgttcagacg
    34741 gccggcgtaa tcgcgcggca aaacttggaa aacggcgttg tgttcgctgt cgacgggcag
    34801 cactgccgcg ccgtttgcac gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    34861 ctctttgttc gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    34921 ccccaccgcc ccgacgatgg cgcacatgac accgctgact tcgtcggcag aggcaacgtc
    34981 aaccaatgcc tgcgcgccgt gtaaaacctg cgtcgccgtg ccgtcgcgtt tcaacagggc
    35041 ttcaagccgg gcggcgtatt cggcatcggc aacgacggca tattcgggtt ggaacgtttg
    35101 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    35161 ttcggggtgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    35221 tatggtcagg acttgtggtg tcataatggg gataacttta taccggatgc cgtctgaagc
    35281 gttttcagac ggcatagaat caatttaaaa ccgacatcat cgccgcatag acgctgataa
    35341 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc agcttgctgc
    35401 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa aaggtcgccg catacgctga
    35461 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    35521 agccggcact tcgtacggcg gtcatgtaca cggccacgca aaccgcgccg ccgattgcgc
    35581 cttcccagct tttgccgggg ctgattgccg gtgcgatttt gtgtttgccg aacgccttgc
    35641 cgctgaaata cgcgcaaata tcggcaaccc acaccaaacc catcacggcg agcagcggca
    35701 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgaccagaa
    35761 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccata
    35821 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    35881 agccgcccgc ataggcaacc acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    35941 taattttgca caaaccgccc atacgggcat attcccacaa ggcaatcagg gcaatcagtc
    36001 cgcaaaatgc agcccacaac cattgcggcg cgtaaaacag catgcccagc atcagcggca
    36061 gcagccacat ggcggtcatt acccgttgtt tcagcatatt cagttccttt gctgttcgat
    36121 aggcagttgc tcggaggtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    36181 atcgtccaaa gccttgccgt caaaatcggg ccacaaaata tcggtgaaat acagttcggc
    36241 ataagccatc tgccagagca ggaaattact gatgcgcgtt tcgccgccgg tgcggatgaa
    36301 caaatccggc tcgggcgcgt ctcccagcat caggtatttt gccagcgtgt cttccgtaat
    36361 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc aacgtccgcc
    36421 gtaatcggcg gcaatgctca gggtcaggcc ggtattgttt gccgtcaagg cttccgcctc
    36481 ttcgatgcct tgcagaatct gccgattaaa gcgttcgcgg ctgcccagta ttttcaggcg
    36541 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    36601 caggaacgaa acttcgtctt cggggcggcg ccagttttcg gtcgaaaagg caaatacggt
    36661 cagatattgc acgcccaatt tggcgcaatg cctcaccata ttttccaatg cgtccaaacc
    36721 gcgtttgtgt cccattatgc gcgggaggaa acgttttttc gcccaacggc cgttgccgtc
    36781 cataatcacg gcgatatgct tgggaatggc ggtgtgttcc aaaacggcct gcgtgctgct
    36841 tttcatgtct gcctttcgcg gttcggcatt caaatgccgt ctgaacgccg aaccgtgcag
    36901 gttaaattgc catcaaatct tcttctttgg cagtcaggag tttgtcggct tcggtaatgt
    36961 atttgtcggt cagtttttga accgcttctt cgccgcgacg tgcctcgtct tcggaaattt
    37021 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatgg
    37081 agacgcggcc ttcttccgct tcgccgcgta cgactttaat caggtctttg cggcgttcct
    37141 cggtcagcat gggcatcggc acgcggatca ggtcgccgac agctgccggg ttcagtccca
    37201 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    37261 cgccgatggt gcgcgcatcc agaagcgtta cgttggcaac ttggctgacg gggaccatgc
    37321 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    37381 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    37441 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaagcggg cagccgtctg
    37501 aaacgcgctt caaacctttc agacggcatg aaaactgtta actgcgaata gtaccgttat
    37561 tcgggcatga cgacaaggca ggcggattgg ggatgccgtc tgaagcggca ggcgtttcag
    37621 acggcatcgt gtccgaccgt cagccgtgtt cccgtgtttc aagcaggctt tggcgcaggt
    37681 gttggcgttc gtgggcatcc agccatttgc ggcgggtgcg ttgcagcagg atgacgaggg
    37741 cggaaatttc ctgacgcata ttggtgctga gccagaggaa gccctgccat tggtagtgga
    37801 ggtgttcggc gagggcttcc agttcggggt tgatggcggt gtcgatgcgg atgcggcggg
    37861 cgtgtctgcc gttgataagg gcgacggttt gttgcaggtc ggtttggagc agtgtgaagt
    37921 ggcggtcaag cagccggatt tcgctgccgt tgagtttggg agattgcagc ttggcggcag
    37981 tggtcaggag cagctcggtg gtgttgacaa ttttacggtg ggcgtgctgc atggcttcca
    38041 tcatggcggg gctgatgcgg ctttcgcccg atgtggcggc gaggtggctg cggcttttga
    38101 ccatgcgtgc gttgatttgg cgcattttaa ccatattctg ctccaaacgt tcgcgcgtca
    38161 tacgcctgcc gttgctgatt tcggcaatca ttttgctgca gtcggtcagg ttgtcggcaa
    38221 gcatgaaacg ccacatcagt gtggatttca gcggcagcag tttggcggcg gcgatggcga
    38281 tggccgcgcc gatgaggacg ttcatcgcgc gcatcaggcc gctgtcgaac cattcgctgc
    38341 cgttgtcgcc gatgagcatg cacatcgtca gccccgccag catagggacg tagccgtttt
    38401 tgccgaccgc cgcccagccg gccagtgcgc ttgccgtgcc gacggtgagg tagaagagga
    38461 ggttgccgtg gaaataatgc tggttcagcc ataaaacgcc caaacccgcg cccagcccga
    38521 tgaccgtgcc gagcatacgt tccaccgcct tggagtaaat cgcgccttga aactggagca
    38581 tgccgaggac gacgaagacg gtcatcccta tccactcgcc gtgttggagg tggagcagcc
    38641 gggcggaggc ggtggcgaac agaacggccc cgccgagccg gacggcgtgg atgaggcggc
    38701 ggtagcggta gcgttcgtag gagttgagcc agcggctgac gaggcggttg cgttgcgagg
    38761 tgttcatatc ggttgccgtc tgaagcggaa atgtgaaaaa gcacaggctt cccgagggag
    38821 ggtctgtgct tggtattggt gccggagaag ggaatcgaac ccccgacctt cgcgttacga
    38881 atgcgctgct ctaccgactg agctacaccg gcgtttttcg ttatgatata tatgaacggt
    38941 tgtttgtgca acttttctgg cgggcggcaa ggcagtgcgc ggtatagtg
//
LOCUS       DAWFIT010000009        34983 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFIT010000009 DAWFIT010000000
VERSION     DAWFIT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.009"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(320..622)
                     /locus_tag="WGQ85_000421"
     CDS             complement(320..622)
                     /locus_tag="WGQ85_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0749921.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(594..881)
                     /locus_tag="WGQ85_000422"
     CDS             complement(594..881)
                     /locus_tag="WGQ85_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0749922.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            961..1566
                     /locus_tag="WGQ85_000423"
     CDS             961..1566
                     /locus_tag="WGQ85_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749923.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            1545..1841
                     /locus_tag="WGQ85_000424"
     CDS             1545..1841
                     /locus_tag="WGQ85_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0749924.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKILYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            1838..2047
                     /locus_tag="WGQ85_000425"
     CDS             1838..2047
                     /locus_tag="WGQ85_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749925.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(2157..3398)
                     /locus_tag="WGQ85_000426"
     CDS             complement(2157..3398)
                     /locus_tag="WGQ85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0749926.1"
                     /translation="MQKWQTELYSTPLWLLQTLLIVSAASAVILFLTRKTRFGREFAY
                     ILRLCLTPKNAVKVLLLITAMVTLLLTEVRLNVLSTFMSKGLYDSMQDLNASAFWMFA
                     AMNAGVVLIRAFNNVVNDFLDQGLAIKWSERLNEVLTARWLADKNYYRLQMRRHAPDN
                     IDQRIQQDAQEFIASTIEFVRGMVNSVVTSLEFAVVLRGLAGILTVFGFDIPHGIVWF
                     VYIFVILATFIAMWIGNPLIRYNYENEKLNGDYRYSLIRVRDHAESVAFYNGEKHEYD
                     QLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNGIKLFPIILQAPRLFAGQIKIGDIQQ
                     TVQAFARLQNALSFFRMFYNKFTAYRARLERLYGFLLSTEEQHSARQPEISEVSDGIA
                     LECKAAAAFARIGGSFCTAEA"
     gene            complement(3686..4333)
                     /locus_tag="WGQ85_000427"
     CDS             complement(3686..4333)
                     /locus_tag="WGQ85_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0749927.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(4330..4698)
                     /locus_tag="WGQ85_000428"
     CDS             complement(4330..4698)
                     /locus_tag="WGQ85_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0749928.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDNTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(4708..5379)
                     /locus_tag="WGQ85_000429"
     CDS             complement(4708..5379)
                     /locus_tag="WGQ85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0749929.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(5544..6263)
                     /locus_tag="WGQ85_000430"
     CDS             complement(5544..6263)
                     /locus_tag="WGQ85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0749930.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALLPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(6347..7660)
                     /locus_tag="WGQ85_000431"
     CDS             complement(6347..7660)
                     /locus_tag="WGQ85_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0749931.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(7739..8611)
                     /gene="hpnD"
                     /locus_tag="WGQ85_000432"
     CDS             complement(7739..8611)
                     /gene="hpnD"
                     /locus_tag="WGQ85_000432"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0749932.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            8782..10644
                     /gene="hscA"
                     /locus_tag="WGQ85_000433"
     CDS             8782..10644
                     /gene="hscA"
                     /locus_tag="WGQ85_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0749933.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            10726..11382
                     /locus_tag="WGQ85_000434"
     CDS             10726..11382
                     /locus_tag="WGQ85_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0749934.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            11418..12179
                     /locus_tag="WGQ85_000435"
     CDS             11418..12179
                     /locus_tag="WGQ85_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0749935.1"
                     /translation="MMGDSVIYYVVQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            12234..12575
                     /gene="fdx"
                     /locus_tag="WGQ85_000436"
     CDS             12234..12575
                     /gene="fdx"
                     /locus_tag="WGQ85_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0749936.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(12862..13449)
                     /locus_tag="WGQ85_000437"
     CDS             complement(12862..13449)
                     /locus_tag="WGQ85_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0749937.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            13662..13859
                     /gene="iscX"
                     /locus_tag="WGQ85_000438"
     CDS             13662..13859
                     /gene="iscX"
                     /locus_tag="WGQ85_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0749938.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(13927..14328)
                     /locus_tag="WGQ85_000439"
     CDS             complement(13927..14328)
                     /locus_tag="WGQ85_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0749939.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            14539..15498
                     /locus_tag="WGQ85_000440"
     CDS             14539..15498
                     /locus_tag="WGQ85_000440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0749940.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15566..16876
                     /gene="tilS"
                     /locus_tag="WGQ85_000441"
     CDS             15566..16876
                     /gene="tilS"
                     /locus_tag="WGQ85_000441"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0749941.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            16927..17712
                     /locus_tag="WGQ85_000442"
     CDS             16927..17712
                     /locus_tag="WGQ85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0749942.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            17722..17919
                     /locus_tag="WGQ85_000443"
     CDS             17722..17919
                     /locus_tag="WGQ85_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749943.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            18181..18534
                     /locus_tag="WGQ85_000444"
     CDS             18181..18534
                     /locus_tag="WGQ85_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749944.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            18546..18853
                     /locus_tag="WGQ85_000445"
                     /pseudo
     CDS             18546..18853
                     /locus_tag="WGQ85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18915..20291
                     /gene="mpl"
                     /locus_tag="WGQ85_000446"
     CDS             18915..20291
                     /gene="mpl"
                     /locus_tag="WGQ85_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0749945.1"
                     /translation="MKHIHIIGIGGTFMGGVAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLSENVL
                     RHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            20498..21550
                     /gene="bioB"
                     /locus_tag="WGQ85_000447"
     CDS             20498..21550
                     /gene="bioB"
                     /locus_tag="WGQ85_000447"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0749946.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(21628..22263)
                     /locus_tag="WGQ85_000448"
     CDS             complement(21628..22263)
                     /locus_tag="WGQ85_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0749947.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            22586..22846
                     /locus_tag="WGQ85_000449"
     CDS             22586..22846
                     /locus_tag="WGQ85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0749948.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(22909..24768)
                     /gene="ilvD"
                     /locus_tag="WGQ85_000450"
     CDS             complement(22909..24768)
                     /gene="ilvD"
                     /locus_tag="WGQ85_000450"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0749949.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(24941..26710)
                     /gene="cysI"
                     /locus_tag="WGQ85_000451"
     CDS             complement(24941..26710)
                     /gene="cysI"
                     /locus_tag="WGQ85_000451"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0749950.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(26737..28551)
                     /locus_tag="WGQ85_000452"
     CDS             complement(26737..28551)
                     /locus_tag="WGQ85_000452"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0749951.1"
                     /translation="MSEHDMQNTNPPLPPMPPEITQLLSGLNAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(28548..29834)
                     /locus_tag="WGQ85_000453"
     CDS             complement(28548..29834)
                     /locus_tag="WGQ85_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0749952.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLAIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(29873..30796)
                     /gene="cysD"
                     /locus_tag="WGQ85_000454"
     CDS             complement(29873..30796)
                     /gene="cysD"
                     /locus_tag="WGQ85_000454"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0749953.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(30834..31568)
                     /locus_tag="WGQ85_000455"
     CDS             complement(30834..31568)
                     /locus_tag="WGQ85_000455"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0749954.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(31584..33044)
                     /gene="cobA"
                     /locus_tag="WGQ85_000456"
     CDS             complement(31584..33044)
                     /gene="cobA"
                     /locus_tag="WGQ85_000456"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0749955.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGG
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            complement(33341..33997)
                     /locus_tag="WGQ85_000457"
     CDS             complement(33341..33997)
                     /locus_tag="WGQ85_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0749956.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            34062..34952
                     /locus_tag="WGQ85_000458"
     CDS             34062..34952
                     /locus_tag="WGQ85_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0749957.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLSKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT         7669 a         9178 c        10097 g         8039 t
ORIGIN      
        1 tatcggaaaa aacagaaacc cccgccgccg tcattcccgc gaaagcggga atccagaccc
       61 caaacgcggc aggaatctat cggaaacggc tgaaaccgaa cggactggat tcccgctttc
      121 gcgggaatga cggcggcagg ggtttcggga ttcccgcctt cgcgggaatg acggaaagtg
      181 gcgggaataa cgaaaggcgg gaatgaccgc gcaaaaagcc gctgccccct tcggacggca
      241 ccggcaacaa aaaaccgcac ggccgaaacc gcgcgggaaa ggatagtcgg gcgcgcccga
      301 taagcagcgg ccgcccctgt tatttcaatt gggcgacata ttggcgcaaa acctcgttga
      361 tgcgcgtctg ccagcccttg ccgccggcgc ggaatttttc gaccacatcg gcggacaggc
      421 gtatggtaac gagttgtttc ggggttttgc ctgtgtttcg tttttgcatt acgccctttt
      481 cttccaattg tttttgatgg gaaaaaagca cctgtgccaa gtcttcgggc agggcttcgg
      541 caatggggcg ggcaagcgca aaatcttcgg cggcaagttc ccgcacttcg ccgtcagcgt
      601 ttgttaagga ttgacgttgc atatttttta acctctcttt tattcgcttt gcgaaaactg
      661 atgacacgga tgccgtcttt tatcggcgta aaacagacaa tgtgcaggcg ttgcgtatcg
      721 cctagataag cagcggcaac ataacgcggt tcggggtaat caaagcggac atcgggcaca
      781 ataacggccg ttgtccagcg tatttgcccg actgattcaa agggcaaatt ccgctcttcg
      841 atattgcgtt gatttttttc ggagtcaaat tcaatcttca ttgcagcttg cagcgtattt
      901 tgtcgttaca ttataagcgg caaaaaacca aaatgtaaat acaaaaaagg aaaccccgaa
      961 atgaccatct atttcaaaaa cggcttttac gacgacacat tgggcggcat ccccgaaggc
     1021 gcggttgccg tccgcgccga agaatacgcc gcccttttgg caggacaggc gcagggaggg
     1081 cagattgccg cagattccga cggtcgcccc gttttaaccc cgccgcgccc gtctgaatac
     1141 cacgaatggg acggcaagaa atgggaaatc ggcgaagccg ctgccgccgc ccgtttcgcc
     1201 gaacaaaaaa ccgccacggc attccgcctc gcggcaaagg cggacgaact caaaaacagc
     1261 ctcttggcgg gctatcccca agtggaaatc gacagctttt acaggcagga aaaagaagcc
     1321 ctcgcgcggc aggcggacaa caacgccccg accccgatgc tggcgcaaat cgccgccaca
     1381 aggggcgtgg aattggacgt tttgattgaa aaagttatcg aaaaatccgc ccgcctggct
     1441 gttgccgccg gcgcgattat cggaaagcgt cagcagctcg aagacaaatt gaacaccatc
     1501 gaaaccgcgc ccggattgga cgcgctggaa aaggaaatcg aagaatggac gctaaacatc
     1561 ggctgaaaaa atacgtttac cacctgttgg tagccatcga ccaactgttc aacgccctaa
     1621 ccggcggcgc ggcggacgaa accctctcaa gccgcaccta tcgcggcgcg cggctcgccc
     1681 aaaagcccaa aacccgctgg aagattttat ataccctgat taacggcgtg tttttcgacc
     1741 gccaacactg tcggcaggcg tatatcagcg aactgaaagg caggcagcac gacgcgcggt
     1801 tcaaccaaag ccgcgccgcc ggggaaaagg ggacgcgatg aactacttcg gcatggtaga
     1861 gtttctgcgc ctgatggcaa tggtgcggcc gccatttgtc ttcttctccg gcacccgcag
     1921 cgaactgcct gcttatcttg acctcgtggc agaactgcgc ctgaccggat gggagcgttt
     1981 tgcaggcagc caaaccttga ctgtgagcag cacatcaaca gcaattcaag ctacgacgac
     2041 cacctgattt ataagttctg accgcaagta gcgtactact tttaaaggca taagataatc
     2101 cctgtttaac agaccattaa acggggataa atttgtgcaa aagctaatac aatttcctac
     2161 gcttcggcgg tgcaaaagct gccgccaatt cgtgcaaaag ctgccgccgc cttacattcc
     2221 agtgcaatgc cgtctgaaac ttcgctaatc tcgggttgtc gcgcgctgtg ttgttcttcg
     2281 gtactcagca aaaagccgta cagacgctcc aatcgggcgc ggtaggcggt gaatttgttg
     2341 tagaacattc gaaagaaaga aagcgcgttt tgcagtcgcg cgaaggcttg gacggtttgc
     2401 tggatgtcgc cgattttgat ttgtccggca aacaggcgcg gagcttgcaa aataatcggg
     2461 aagagtttga tgccgttggt gaacatatcg ttaaagccgc ttaagcagac gctttgccgc
     2521 gcaatgcgcc aacggttgcg gataatggct ttaaagcggt cggaaagctg gtcgtattca
     2581 tgtttttcgc cgttgtaaaa cgccacgctt tcggcgtggt cgcgcacgcg gatgagggaa
     2641 taacggtagt cgccgttgag tttttcgttt tcataattgt aacgaatcaa agggttgcct
     2701 atccacatgg cgataaaggt cgccaaaatc acaaatatat agacaaacca aacgatgccg
     2761 tgcggaatgt cgaagccgaa cacggtcagg atgccagcca agccccgcaa aacaacggca
     2821 aattccagag aagtaacgac cgaattgacc atgccgcgca caaattcgat ggtcgaagcg
     2881 ataaattcct gcgcgtcctg ttggatacgc tggtcgatgt tgtctggcgc gtggcggcgc
     2941 atttgcaggc ggtagtagtt tttgtcggca agccagcgtg ctgtcagcac ttcgttgagc
     3001 cgctccgacc atttaatcgc caagccttga tcgaggaagt cgttgacgac gttgttaaac
     3061 gcccgtatca gcaccacgcc cgcgttcatt gcggcaaaca tccaaaatgc cgaagcattc
     3121 aaatcctgca tcgagtcgta aagccctttg gacataaagg tactcaacac attcagccgt
     3181 acttcggtta acagcagcgt aaccattgcc gtaatcagca gcaatacttt gaccgcgttt
     3241 ttcggtgtca gacaaaggcg caggatatag gcaaactccc gcccgaagcg cgttttccgt
     3301 gtcaaaaaca gaatcaccgc tgaagcggca gaaactatca agagagtctg taaaagccaa
     3361 agcggtgtgg aataaagctc ggtttgccat ttttgcatgg gaaatctctt acggtatcaa
     3421 tgccgtctga aaagacgggt acagttgatt ttttgatgaa gtttggggaa gttttgcagg
     3481 tcagggcata ttgcgtgtta atttaccata caaccacgcc ggaattaagt ttaaatttga
     3541 ataaaaggtt cgggttctgt aaaatacaga acccgaacct tgttcggata ttgaaaccgg
     3601 ccgcccgatt ttgggcggtg cggcttgcaa gtatcaagat tcgcatatgc cgtctgaagc
     3661 tcggagaggt tcagacggca tatgcttatt tgggctgctc ttcgacgaat ctcggacctt
     3721 tcaagatgcc gttgtgagaa tagggcgaca gcaggttgta tgcggcggtt ttggaaacct
     3781 gataaccgcg gtcggtcagg ctgttggcaa tctgattgac cactgcgctg accaaagccc
     3841 ccaacaggcc gctgttgctg ttgttgctgc cttcgcggat gctggccgaa cccgaccaca
     3901 actcttttcc gttgcgggaa tcgaccagcc gtgctttggc ggatacggtc gtcacgctgt
     3961 ctaaaatttg atatgaagtg ccgtattcgg taaccgtaat gtacaaaacc gcatcattgc
     4021 cgaaaatctg atgcagtttt tccggccgga cggcgtgaat atcggcggca ttggtcaagc
     4081 cgttttgttt gaaggtttcc tccacgactg cggcggggaa gacgtaatag ccggcttcgg
     4141 aaagcggcgc ggcggtcgaa gccagtacac cccatgttcc gttgacatcg ggcgattcgt
     4201 tcagcggcgg aaccaccaaa attgaagccg gtttgctttc cttgaatgac gtgtagtcga
     4261 aatcgggcgc tttttgaact tggcaggcag acagcgccaa cacggcggca agccctaaaa
     4321 tcaaaggttt catcgcttgc ctcctttacc tgttttcatc aggaagtcca taaatacgcc
     4381 cgattcggga aacagccttt tctcttcttc aaactggcgg aacgcgccct ctttgtctcc
     4441 cgaacgggaa agcagcagtc ccagatgggc gtgcgcaccc ggggcggcat tcattttttt
     4501 gttgccggct tccacaaagt atttttccat cttttcggtc tgcttgccca acgaagtgtt
     4561 gtcgtttttc aaaccttcat agacggtatc gggatagccg ccgtaataat acagggattt
     4621 ttgcccgttg ccgccgcagg cggtcagagc caagaccgcc gcacacagcg acaaacggct
     4681 caaggttttc ggattcatca tttctcctta acggttgggt tgccatgcgc cgttgtcaac
     4741 agcctgaacc agactgttga cggcttcgcg gattgccaag tctaaaactt tgccgttcaa
     4801 agtcgcatcg tagccggaag tgccgccgaa accgatgatt tcacggttgg aaagtgcgta
     4861 ttcgcccgcg ccctgtgcgg aatagacgat ttcggaagta ttgacgttga cgatattcag
     4921 agccactttt gcataggcga tttgcgattt gccgcgaccc aaaatgccga agagctgatg
     4981 atcgccgaca tctctgcgtc cgaattcggt tacatcgccg gtaacgacat aatctgcgcc
     5041 tttcaggtta tgcgctttgc cggaaatgcc ggattcctgt tttaatgcga tcaaattggt
     5101 gcggttcagt acgttgaagc ggttggtctg ttgcaggtgc gttaccagaa tggtttttgc
     5161 ctggctgccc aaacggtctt ccccgtcgga gaaaatgcct ttttggaagc tggagcggtt
     5221 gtcgaatgtt ccgacggaaa tcggggtacg aacaccgtga tattgcgtat tgtaggaggc
     5281 gacttttgct acctcgagac tgcgtgagga ttcggtcgca cagccggtca gtgaaacggc
     5341 agcggcggca aggacaacgg cggtggaaac ggttttcata aaatttaccc taaggtcaag
     5401 ttaaggaaat aacgggttgt cattattgtc cttatgtaaa tttaagtcaa ggtgtttgtc
     5461 tgtgcgggac ggatgcgcac ggaaggtacg gatatttttc aaacggaatc gcggcgcggg
     5521 cgggatgcct gccttttccg gatttagagg taaacgattt cgccgctccg ccctttgctt
     5581 tcggcacttg cccaccagac aaatgcgggc agcacgtccc cgtagctttt gcgttcgctt
     5641 ttggcttcgc ccggatggga tttgatgcgt tgcggggaat tgatgggtcc ggggacgagg
     5701 acgttggcgc gcaggttgcc gaagcgttcc cattcgtcgg cggcgacttt gcacaggtag
     5761 ttcaacgcgg ctttggacgc gccgaagccg ccccagtagg ctttgggggt ttccccgtgg
     5821 ctttcgccga cgaagataac ggatgcgtcc ggcgactgtt tcagcagggg aaggagggcg
     5881 cgggtcagcc ccataggtgc gacggtgttg atgcggtatt ggttgaccca ttcggcgacg
     5941 gtttggaaat ccagcggcga gagggcgtaa aaatagccgg cgcagtggac gatgccgtcc
     6001 agtttgcctt gcgtggcttc ggcaatggtg gcggcgaact gattgaattc tttttcttcc
     6061 gcactcatca ggtcgaaacg gatggcgaag ggttcggggt gtccggcttc gacaatcgcg
     6121 tcatagactt tttccagttt tttctgatgg cgtgccacca gaatcacggt tgcgcctgcc
     6181 gccgcatagg ctttggcgac ctgttcgccc agaccttgcg atgcgccggt aactaagatg
     6241 gttttgttgg acagtgtcgc catacttttt cctttttggt ggtcggtcgg gggtatttta
     6301 gcgttttgcc gcaccttgta aagcgtcccg ataccagccg gcgttttcag acggcatcgc
     6361 tcaggctttg caggcaggtt tccgccgccg cgaaacctga ttgtacggcg gcttcgagcg
     6421 tggcggggta gtccgggtgg aggtagtcgc cggcggggaa gatgcggtgc cggtgcagcc
     6481 acgacaaatc cggcggcggg gcatcggctg cggttgtggc gcgtttttcg gtgatgacgc
     6541 gcacggcttc gggttcgccc aaatgcggaa ggatgcgttt gaggtcggcg tgaactttgt
     6601 ccgcccacgc tcggtttgta aacgcgccga cgcggtcgga aacgctgatg acggcggaca
     6661 cttcgttttc aggcagtccg agcctgcccc ggcaaagcag ccattgcacc gtgccgtcgg
     6721 caaggccggt cagcggggcg ggcaggcgga cgggttcggc gtagcgcaga tagacggtgg
     6781 tgatggcgtg gtagcgaagg ttttgatatg ccgtctgaac gtgttcgggc gtgccttcgg
     6841 gcaggagcgc ggcggcgtgg tagggcgcgg tggcggggac ggcggcatcg aaagcttcgc
     6901 cgttgacgag cactttcccg tccgggaggg tgttcagacg gcatacgcgc gtttcgaggc
     6961 ggatgtccgc gccgagccgt tgaagatccg ccaaggcggg ttcggcgacg attgcgccca
     7021 aatcctgctt gggtaggaga tagtcgctgc cggatttttt cgtcagcacg ccgtcggaca
     7081 aaacgttgca caacacgcgc aggcttgcgg tttccaaagg cgtgttgagc gcgccccaaa
     7141 ccaagggctg ccaaaactgc atcacggcgg cacgcggcac gttccgctgt ttcagccatt
     7201 gcgccactgt cgtgtcgggc tgtccgaggc gtgcggactt ctgcaaatcg gacatatcgg
     7261 caagcagttt ggctttgaat gcagtcggtg cacgccgggc aagcagcacg ccgcccaaaa
     7321 tatgcagcgg cgcgggcagg gggagggcgc ggaactgcaa accgccgtgc atatgccagt
     7381 gcagcggtac gcgcaaaaag gcggcacggg gatccgaacc gatggttttc atcaggcgca
     7441 acacgccccg gtatgcgccg agcaaaatgt gctgcccgtt gtccaaaaaa ccgaaaccgt
     7501 cggtatttcc ggccagtgcg cgcgccctgc cgcccgcctg ccggccggct tcaaacaggg
     7561 taacgtcggc gtgccgcgcc aaggtgacgg cggcggacag tcctgcccag cctgcgccga
     7621 tgacggcgat tttcgggcgc ggatgcggcg tgttcatcat ttattcctcc aatggttgaa
     7681 accccgtttt tcggggcggc gggggttggg ttttcctgaa gggcgggcgg tatgcgcctt
     7741 atgcccgttc cggcgtgccg gggcgcggtt tgaatccgaa taaccaggtt ttcagggcaa
     7801 tgcgtttttt gcgcggcgaa gggagggcga ttttgtattt gaggacgttt tgcgcgccgt
     7861 cccggtcgat ttcgttcaat agctcgtaat aaaccgccgc cataaccagt ccgacttttt
     7921 gggctttttt atcggcagcc ggcagcagcg atacggcttc gcggtaggtt tcacgggcgc
     7981 gtttgatttg gaacgccatc aattcggcaa aattgcccgt cgggctgcat tgcaaaatca
     8041 cgcttgcggg gacatcaaac cgctgcattt cctccatcgg caggtaaatc cgtcccctgc
     8101 gcgcgtcttc gccgacatcg cggatgatgt tggtcagttg cagcgcaagt cccatcttgt
     8161 cggcgtattc cagcgttttg ccgtctgaaa accccaaaat ccgcgcaatc aggcagccga
     8221 ctacacctgc aacacggcgg cagtacagtt tcaattcttc aaaactgccg taacgcgccc
     8281 gaaccaaatc catctgcatc ccgtcgatta aggcttccag ttcatatttc ggcagcttga
     8341 aggtttcctt aacctgccgc aaggcctgat tgacggggtg ttccggcatc gcgccgccga
     8401 ataccttgtc caaatcgccg cgccaccagt tcaatgtcgc ctgtgcaaca tcggggttgg
     8461 aacattcgtc aaccacatcg tccaattcgc ggcaaaaagc atataaaacc gttaccgcat
     8521 cccgtttttc ctgagtcagg aaacggaagc ccgacaaaaa actggagcgg ctttcttctg
     8581 ctttttggcg gcaatagtcg agtcctttca cgatttatat tcctaatgat gggagggaaa
     8641 ggcggatttt atcggcattt ggcggtagag ggcaatttcg gcggcacgac ccaatcctta
     8701 gcggtttgct caactatcgg cgcgaattct gttaaaatgc cgacttcctt cctttacaca
     8761 ccgcaccgac aggcagaatt tatggctctt ttgcagattt cagaaccggg tatgtccgcc
     8821 gccccgcacc gacaccgttt ggcggcaggc atcgatttgg gtacgaccaa cagcttggtc
     8881 gccaccgtcc gcagcggcag tgccgcctgt ctgcccgatg ccgaagggcg cgttaccctg
     8941 ccttccgtcg tccgctatct ggaaaacggc ggcattgaag tcggcaaaac cgccctgtcc
     9001 gcccaaaaaa ccgacccgct gaacaccgtc agctccgcca aacgccttat cgggcggacg
     9061 cttgccgatc tgcatcaaaa tacgcattac ctgccttacc gtttcggtga caatcaacgc
     9121 gttatcgaac tgcatacgct gcagggggtg aaaacgcctg tcgaagtgtc ggcggaaatc
     9181 ctcaaaaccc ttaaatcgcg cgccgaagaa accttgggcg gcgatttggt cggcgtggtg
     9241 attaccgtcc ctgcctattt cgatgatgcc caacgccaag ccaccaaaga tgccgcgcgt
     9301 ctggcgggtt tgaacgtatt gcgcctgctc aacgaaccca ccgctgccgc aatcgcctac
     9361 gggctggaca acgcctcgga aggcacgttt gtcgtgtacg acttaggggg cggcacattc
     9421 gacgtatccg tattgcaact gaccaaagga ctgtttgaag tcaaagccac cggcggcaac
     9481 agcgcgttgg gcggcgacga tttcgaccac cgcctgttct gccgcctgct cgaacaaaac
     9541 ggactctccc aactcaacga acaagacagc caactcctgc tctcgctcgt ccgcgccgcc
     9601 aaagaacaat taaccacgca aaccgaagcg cgcattcagg cgacgctttc agacggcatg
     9661 gcaatcgaca caagcatcag tcgcgccgag ttccacaacc tgacgcagca tttggtgatg
     9721 aaaacgctcg aaccggtcac acaggcgttg aaagatgccg gtgtcggtaa aaacgaagtc
     9781 aaaggcgtga ttatggtcgg cggttcgacc cgtatgctgc acgtccaaca ggcagtcgcc
     9841 accttcttcg gacaaacccc gctgaacaac ctcaatcccg acgaagtcgt cgcgctcggc
     9901 gccgccatac aggcaaacgt cctcgcaggc aacaaaaccg acggcgaatg gctgctgctg
     9961 gacgttacac ctttgtcgct cggtttggaa acctacggcg gcttggcgga aaaaatcatc
    10021 ccgcgcaatt ccaccatccc caccgcgcgc gcgcaggact ttaccacctt caaagacggt
    10081 caaaccgcga tgacgataca cgtcgtgcaa ggcgagcgcg agctcgtttc tgactgccgc
    10141 agccttgcca aattcaccct gcgcggcatt ccaccgatgg cggcgggtgc ggcgcgtatc
    10201 cgcgtaacct tccaaatcga tgcggacggg ctgctgtccg tttccgccca agaacaaagc
    10261 accggcgtac aggcgcaaat cgaagtcaaa ccttcctacg gcttggacga cggcgcgatt
    10321 acccgaatgc ttaaagacag tatggacaac gccgccgaag atatggcggc acgcgcccgc
    10381 gccgaagccg tagtcgaagc cgaaagcctg accgatgccg tcaacgccgc cctcgagttg
    10441 gacagcgatt tactggatgc caaagaattg caacagatcc ggcaaggcat cgccgatttg
    10501 caaggccgtc tgaaagacgg aaaagccgaa gacatccgct ccgccgtcgc caaactcagc
    10561 cgcagcaccg acaacttcgc cgccaaacgc atgaaccgca atatccaacg cgcgttgact
    10621 ggtcaaagcg ttgataatat ttgacggcaa atggtttcag acggcattgg ataaagaggt
    10681 cgtctgaaaa aatatcatct cacagaaata aaataaaaaa caacaatgga aaagtcaggc
    10741 attgtctatc tgatgaaaac cgtcatcaag ggcgtgtata aaatcggcat ttcggatgta
    10801 agcaattttg aaggcagaat gcgccatttg gaaaacaacg gttatgcgaa cgttgccggg
    10861 ttggaacgca tcctcgccgt caaaaccgac aattacaaag aaaaagaaaa cctgctccat
    10921 gaaattttta gtaaaagcag gataggcgat accgaattgt tcgccgtgga cgagaatctt
    10981 gtaaaacgtt tgtttttatc gcttcgcggc gaaatcgtgt tcccgaaaaa cgaaacggcg
    11041 gaatcggaat ttgaaaaaag cgtccacgaa cgcaggcagg aagggaatgc cggatccggt
    11101 cgcaaacaac tgcttgattt ggtacggcgc ggacaccggg aataccctta cgcgctgccc
    11161 cggcttttgg cgggcgcggc atcctacaag ccgaaaaaat cgaaaatccg cctttttaaa
    11221 gaagcatatt tacgcaaaag cggtacgagg ctgaccgacg aaattgcaga cggcatccat
    11281 atttacacct gtttttcgcg ggcggatttg gaaaaagcct attccgaata tttggaactt
    11341 ttcaaatccg aatcggatgc cgaaggcaga aaaccacggt aaggtgcaaa cagataccgt
    11401 acacgttgag gagcagtatg atgggcgatt ccgtcattta ttatgtagta caggcagacg
    11461 aaccggtaaa ccgtgccggc gaacgcgccc gtaaaacatt caaatacttt tggcgcgagc
    11521 ttttttggga acgccgccgc atcattcccg ccttagattt tgccatggtc aaagcccctt
    11581 ttttccaaga cggcgaagac ggcgaaattt gcgaacatat gtggatcgac gatatttatt
    11641 ttgacggtct ttacatttac ggtgtgctga acaataaacc cggcgaactg actaatgtcg
    11701 aacaaggcga aagcatttgc gttccggttg acgacatcag cgactggatg ttcgtgtgca
    11761 acggcattcc ctacggcggc tttaccatac aggcaatgcg tggacagatg acggaagagg
    11821 agcgtaccga acacgatgcc gcatggggaa tcgatttcgg cgatcccggg caggtattgc
    11881 tggtgtatga agaaaaagaa catcccgaaa atctggaaga gcatccgatg tgccggaact
    11941 gtattgacga ttttcggcaa cagttgtccc aaaatccgga ttttctgcat gagcaggacg
    12001 aagacggcta tacgccgctt catcatgaag ccatcgcagg aaatgcactt atggttcaag
    12061 ccatgcttga atacggcgca aatcctgcct caaagacatc ggaaggctat accgccctcg
    12121 attttgcccg cctgacgggc tggcaaaatg ttgccgacct gctcgaaccg cgacattagg
    12181 cagacagttt tccgaaaacg aacacaaaca ctttttacag aaagacaata aaaatgccca
    12241 aaatcaccgt acttccacac acgacattat gccccgaggg tgcagtcatc gataacgcac
    12301 ccgaaggtaa aaccgtcctt gacgtgctgc tcgaccatga tatcgaagtc gatcacgcct
    12361 gcgaaaaatc ctgcgcctgc acaacctgcc acgtgattat ccgcaaaggt ttcgacagtc
    12421 tggaagagcc gaccgaattg gaagaagacc tgctcgatca ggcttggggt ttggaagccg
    12481 attcgcgcct gagttgtcag gcggttgtcg ccggcgaaga tttgattgtg gaaatcccca
    12541 aatacaccat caaccacgcg cgcgaagaac actgaaaaca ggccgtctga agccggcacg
    12601 cttcagacgg cattgttgcg cggataaggc gcaatcgccc gaaaacaggc gttcgtacag
    12661 gcggaacttt cgattctata gcgaattaac aaaaatcagg acaaggcggc gagccgcaga
    12721 cagtacaaat agtacggcaa ggcgaggcaa cgctgtaccg gtttaaattt aattcactat
    12781 atattgattt ttatcggttt tctgacggaa cggattggtg cggcatccga ggcggattac
    12841 tcgggcgcga tgcacgggta tttatcggtt ttgcagccgg aaaaaccgcc ggcgggttac
    12901 tttcagggcg gcggtcgggc ggtatgccgg atagcgttcc actttcgctt ctatattgat
    12961 tttgattggt tttctgacgg aatgacccga tgcggcatcc gggacggctt gtgttttttc
    13021 ctgcccgcct gccggatttt cccatccttg cgtgaaaccg aaagagacgg cggcgcggcg
    13081 gacaagctcg agatagcgtc cttcaagctc cgggcaggcg gcggacacgc tttctgccgt
    13141 aaccgtgaag ccgccgcccg acccggaaag gcgttccgca atctgcgtgt agatacgcca
    13201 acgttgggcg cgcaacattg ccgcatcgca accggcagcc gctttatcca aggcaatcag
    13261 gccgcgccgg ttttggtggt gaaggcaaac cgttactctg gagaggatat gttgcccgtc
    13321 caatatttga tacagtttgc gtatcagcag aatcaggcgg tcaaactcct ccatctccga
    13381 cagggaatca ggatggtcgg aagcggtata aaccagtttg gacaattttg cggcaaacat
    13441 attgttcatc aatcttcctt gtcggttgaa accccgctct tcggggcggt agaatcagat
    13501 ttgtttggga gggacaactc ctcccagatc aggacgacac ataggctggt gcttgatgtg
    13561 ttgtccggcg agttgaaaca ttcagccatc ctcaaggtgc ggcagttttg ccgaaacata
    13621 ttctacacgg cttcaatgcc ggacgataaa aggaaattca tatgaaatgg accgacaccc
    13681 ggcgtatcgc cgaagaactc tatgacctgc acggcgaaac catcgatccc agaaccgtgc
    13741 gctttaccca actgcgcgac ctgattatgg cattgcccga atttgacgac gaccccgccc
    13801 gttgcggcga acgcatcctc gaagccgtgc agcaggcatg gatagacgag gcggaataag
    13861 tttcgggaat gccgtctgaa atgcggcggt acgcggttcg tgcttctgtt tgcagcggga
    13921 atggttttac cagtctcctt ttttcagcct gtccagttgg cggcggtcgc gcttggtcgg
    13981 tctgccgtcg ggataggcgg aagtgatgcg gctgaattgg tcgagctgtt tgcgctcttc
    14041 cctcaatgtt gccgttttcg cgtcctcttc atacagaagc cgcgcctcgg atgccgggcg
    14101 gcgttggtgg ttcaaacctt taaccttgat tttataggga agggaattga gcgtcaggtc
    14161 gataatatcg ccgatgtcta tggttttact gtttttgacc ttcgagccgt ttacttgaac
    14221 cctacccagt tcgatgtgct tttgcgcaag ggaacgggtc ttgaaaaaac gtgccgccca
    14281 aagccatttg tccagccgca tggcggaaga atcgtgcttg tctttcatac gattttgttt
    14341 gaaataattg aatttgtttc aagtttagca taagatacgc cgcctataac tagtatatat
    14401 gcactaatcc actgttttcc atgctgtccg aacacaaaaa gagggtgtgg aaaagccgtt
    14461 ttggacaata aattaactgc ggaatatgca caaatagcgt atgatagcgg cagaatctgt
    14521 tgatgagagc ttcattctat gaaacctgtt tttttggatt ttgaacaacc catagccgaa
    14581 ctgaccaaca aaatcgatga gctgcgtttc gtccaagacg agtctgccgt cgatatttcg
    14641 gacgaaatac accgtttgca gaaaaaaagc aacgacctga ccaaatcgat tttcagcaaa
    14701 cttacgcccg cccaggtttc acaggtttcc cggcatccgc agcgtcccta tactttggat
    14761 tacattgagg cactgtttac tgattttgaa gagctgcacg gcgaccgcca ctttgccgac
    14821 gattatgcga ttgtcggcgg attggcgcgt ttcaacggac aaagcgtgat ggtcgtcggg
    14881 catcagaaag ggcgcgacac caaagaaaaa atccgccgca acttcggtat gccccgtcct
    14941 gaaggctacc gcaaagccct gcgcctgatg aagacggcag aaaaattcgg cttgcccgtc
    15001 atgaccttta tcgatacgcc gggcgcgtat cccggcatcg gcgcggaaga acgcggacag
    15061 tcggaagcca tcggcaaaaa cctgtacgaa ctgacgcgcc tgcacgtccc cgttttgtgt
    15121 accgtcatcg gcgaaggggg ctcgggcggt gcgttggcgg tcgccgtagg cgattacgtc
    15181 aatatgctgc aatattcgac ctattctgtt atctcccccg aaggctgcgc ttcgattttg
    15241 tggaaaaccg ccgaaaaggc ggcggatgcg gctcaggctt tgggcattac tgctgaccgc
    15301 ctgcaaaagc tggacttggt cgataccgtc atcaaagaac cattgggcgg cgcgcatcgg
    15361 gatttcgggc aaaccatgaa aaacgtaaaa gccgttttgg aaaaacaact gcacgaagcg
    15421 caaagcatcc cgcttgccga tttgctttcg cgccgtttcg accgcattat ggcttacggc
    15481 aaattttcgg aacaataatt caggtagaac aagcagcaag cagtttgtct gaaactgctt
    15541 gctttttctt tatcgggacg gaaccgtgct gactttagat gcgtttgagc aatgcttgaa
    15601 ggattgtttt cctcaaggtc tgaatggaaa aaaaacagcg gtggcattaa gcggcggctt
    15661 ggattccgtc gttttgctgc atctgcttgt ccgcgccggg aaaaaaggcg gttttattcc
    15721 ggatgcattg catatccatc acggcttgag tccccgtgcc gacgattggg cggatttctg
    15781 ccaaaactat tgcgatatgc tcggggtggg gctggaaacg gttaagatct gcgtggaaaa
    15841 aaacggtttg ggcatcgagg cggcggcgcg gcaaaagcgt tatgccgcgt ttgccgaaaa
    15901 aggctttgac gttttggcgt tggcgcacca cagggacgat caaatcgaaa cctttatgct
    15961 ggcggtcgcg cgcggcggcg gtttgcgcgc tttggcggct atgcccgccg tccgcccttt
    16021 tggggaaaaa ggcatcattt ggcggccctt gctgcctttt tcgcgccaag atatatggga
    16081 ttatgcccaa aaacacggtt tgccgaatat cgaggacgaa agcaatagcg atacggctta
    16141 tttgcgaaac cgcttccggc accgtatttt gcccgaactt tcggcgcaga ttccccattt
    16201 cgggcggcat ctgttgaaca atgtccgtgc cttgcaggag gatttggctt tgttggacga
    16261 ggtcgtcgtt caagactgcc gttgggtttg cggagccggt tatttcgata cggcgcggtg
    16321 gctgacgttt tccccgcgcc ggaaaaccca tattttgcgg cattttttga aggaaaacgg
    16381 cattcccgtg ccgaatcaga atgcccttgc cgacattgcc cgggttttga cggaggcaaa
    16441 aaccggacgt tggaacgtgc aaggctttga attgcatcat tatgcaggca ggctgtttgt
    16501 gttccgaccg gaaaaaacgg ataaactgcg gtttttgaaa gacaggcaga taagcggaaa
    16561 tttaagggaa atattggcgg ggcagggatt tgttttaaaa cgtcatccgt tcggacttcc
    16621 cgggcatctt ttggagcagg acggaatctt gcggacggtt gcggcatcgg acaggctggc
    16681 tgtcggcggt atccataagg atgtgaagaa aatccttcag gaaaagcggg ttttgcccgc
    16741 gctgcgcccg ttttggccgg tcgtcgccga caaggacaac cgtccgctgg cgttggcaaa
    16801 ttacggtacg gattttcaaa tctcggtttc agacggcatt ttgcccgtcc atccggattt
    16861 tcctgtttta ttttgatgga atcggaatcg ccgttaccta ttatatattc ttgaaaaggc
    16921 ggtaaagtga agcatatcag ttcggcaaac aatgaacaca tcagacacct gcaccgcctg
    16981 ttgtctcaag ggaagttcag acggcaatac gcccaaaccg ttttggaggg cgtgcacctg
    17041 cttcaggttt tcctgcaatc cggcggaagg ccagtcgggg tatatattcc cgaagcgaaa
    17101 atgccgtctg aagaagtccg taaattgacg gcggttttgc cggaagacgg ggttttttcc
    17161 gtttcagacg gcatattgaa gaaaatcagc agcctgactt gcgcggatga tgtgcttgcg
    17221 ctgattgata ttccggatgc gggtgctttg ccggccggcg gcgactgtgt ggttttggac
    17281 ggcgtgcaag atccgggcaa tgtcggcacg gtgttgcgga gcgcggcggc ggcaggaatc
    17341 ggcgcggtcg ttttgggcaa aggttgtgcg gacgcgtggt cgcccaaagt gctgcgagcc
    17401 ggaatgggcg cgcatttctt gtcggagatt tatccgcagg cggatttgga aatatggttg
    17461 gcgcgttatg aagaccgtgt gtttgccacc gcgttgcgtg aggaaaaaca ggcggttctg
    17521 tataacgaag atttgcgcga accgacagcc tgggtgtttg gcaacgaagg cgcgggggtc
    17581 ggtaaagcag ttttagacag ggcggacaag tgtgtcagga taccgatgca cgatgcaacc
    17641 gagtctttaa atgtcgcgat ggcggcgaca atctgcctgt ttgaacaaat gcgccaacgg
    17701 gcggcgtatt gaggaaaaga aatgccgtct gaaaaaatct attacggcgt attgattttc
    17761 ttatgtatcg cttctatgct gctgtcgccg tttttttatg cgggtgcttt gaagcccaag
    17821 aaggcggcat tgcggaagga cgggcagtgg aaactcatct tattgtccaa taccgtggcg
    17881 gcggcggttt tggcttgggt gtggtggaaa tggttttgac agatattgct caaaatcgtg
    17941 ctaatggaat ccgaacaaat aaagaatttg gtaaaaaatt tgttaaatca acgaattaaa
    18001 gttttgtgga aaacaaaaca gctctaagca aatagggcgt ttgtcggtaa atacggaaga
    18061 gttgcggcat tatcgggcat ctttaacaag tagtgccgtc ttgacaggca atcggttttt
    18121 atgggcagct tgcaaaatcg cggatataaa attgcaaatc ggttaaagtg tggggacgct
    18181 atgaaaaatt gcgaattttt ttatgacccg acaagggcaa tctatgatag cggtgcagat
    18241 tatttaacca gagaaaaaca tagattcgtc gtaattgcaa atagtgcttg ggggctattg
    18301 cttaatttat cttgttatta tgacgaggtt ttggaaaagc ggaaaatacc gttcggcaaa
    18361 caggaaattg atgacgatat ggacaaagtg tccgcccata agcggaagtt taaagatatt
    18421 tctgaaatca aagtagggga tggttgggaa tacccgttca attatgagca gggaatgaaa
    18481 gaattagatg aagtgctatt gaaatacatt cccttttttg aagaagaacg ataaaggagg
    18541 ttgatatgcg cgtatctaaa ataattggaa gtatgttgct tgttacagcg gttcagaccg
    18601 tattttcggc aaatgtttac gcgtgccgcc ataatggtaa aaccagttac agccaaactc
    18661 cgggaaaaca ttgtaccaac gcgggtttgg ggggcgggac cgggtgtaca gttcggttag
    18721 accggcagta aaagacaggg cggaagacgc aggagttggc gattattcgg acacggtgag
    18781 ggacgaacac gtccaaattc cgaaagggaa tgcacagaaa gacggttcgg atgccggcat
    18841 caggccgcat tgattgaaac cgaatcaacc atgacggtgt tgaacaatag aatttgaacg
    18901 attagggaac tctgatgaaa cacatccaca ttatcggtat cggcggcacg tttatgggcg
    18961 gagtggccgc cattgccaaa gaagcggggt ttaaagtcag cggttgcgac gcgaagatgt
    19021 atccgccgat gagcacccag ctcgaagcct tgggcatagg cgtacacgaa ggcttcgacg
    19081 cggcgcagtt ggaggaattt aaagccgacg tttacgtcat cggcaatgtc gccaagcgcg
    19141 ggatggatgt ggttgaggcg attttgaacc gcgggctgcc ttatatttcc ggtccgcaat
    19201 ggctgtcgga aaacgtgctg cgccatcatt gggtactcgg tgtggcgggg acgcacggca
    19261 aaacgaccac cgcgtccatg ctcgcatggg ttttggaata tgccggactc gcgccgggct
    19321 tccttatcgg cggcgtaccg gaaaacttta gcgtttccgc ccgcctgccg caaacgccgc
    19381 gtcaagaccc gaacagcaaa tcgccgtttt tcgtcatcga agccgacgaa tacgacaccg
    19441 cctttttcga caaacgctcc aaattcgtgc attaccgtcc acgtaccgcc gtgttgaaca
    19501 atctggaatt cgaccatgcc gacatctttg ccgatttggg cgcgatacag acccagttcc
    19561 atcacctcgt gcgcaccgtg ccgtctgaag gcttaatcat ctgtaacgga cagcagcaaa
    19621 gcttgcagga tactttagac aaaggctgct ggacgccggt ggagaaattc ggcacggaac
    19681 acggctggca ggccggcgaa gccaatgccg acggctcgtt cgacgtgtta ctagacggca
    19741 aaaccgccgg acgcgtcaaa tgggatttga tgggcaggca caaccgcatg aacgcgctcg
    19801 ccgtcattgc cgccgcgcgt catgtcggtg tcgatattca gaccgcctgc gaagccttgg
    19861 gcgcgtttaa aaacgtcaaa cgccgcatgg aaatcaaagg cacggcaaac ggcattaccg
    19921 tttacgacga ctttgcccac catccgaccg ccatcgaaac cacgattcaa ggtttgcgcc
    19981 aacgtatcgg cggcgcgcgt atcctcgccg tcctcgagcc gcgttccaac acgatgaagc
    20041 tgggcacgat gaagtccgcc ctgcccgaaa gcctcaaaga agccgaccaa gtgttctgct
    20101 acgccggtgg cgtggactgg aacgtcgccg aagccctcgc gcctttgggc ggcaggctgc
    20161 acgtcggcaa agacttcgat gccttcgttg ccgaaatcgt gaaaaacgcc gaagcaggcg
    20221 accatatttt ggtgatgagc aacggcggtt tcggcggaat acacaccaaa ctgctggacg
    20281 ctttgagata gcccgggcga tgccgtctga aagcccttca gacggcatcg cccggctgcg
    20341 cggcacaaag gcggaaaaac cgtttgcccc gtattttcaa acgcgttaca cttgccgccg
    20401 ctgttttcag ccatttgatt acccgcaacc gccgtcattg cgccggcggt ttgcctgtca
    20461 gcgtcattgc gccgctgtaa atacgaaaga acacattatg accgtatccc ccgtcgcctt
    20521 gcgccgtaag accgagtgca agcctcatcc caccgcgcgc tattggaaaa aatgcgatgt
    20581 cgaagccctg ttcggacttc ccttcctcga cctcatttac caagccgccg aaatccaccg
    20641 ccaaaatttc aacccgcgcg aaatccagct ttccacgctg ttgtccatca aaaccggcgg
    20701 ttgtcccgaa gactgcgcct attgtccgca atcggcgcac cacaatacca atctgggcaa
    20761 agagcagatg atggatgtgg atgaaatcgt cgaaaaagcc aaaatcgcca aatcgcgcgg
    20821 cgcaagccgg ttttgtatgg gcgcggcgtg gcgcggccct aaacccaaag acgtggagac
    20881 ggtttccgca atcatcaaag ccgtcaaggg cttgggtatg gaaacctgcg gcacgttcgg
    20941 tatgctcgaa gaaggtatgg cggaagactt gaaagaggcg ggcttggatt attacaacca
    21001 caacctcgac accgaccccg accgctacaa cgacatcatc cacacccgcc aacacgaaga
    21061 ccgaatggac accttgggca aagtccgcaa cgccggtttg aaagtctgct gcggcggcat
    21121 cgtcgggatg aacgaaaccc gcgccgaacg tgccgggctg attgccagtc tcgccaatct
    21181 cgacccgcag cccgaaagcg tgccgattaa ccggttggtc aaagtggaag gcacgccgct
    21241 tgccgatgcc gaagatttgg actggacgga atttgtccgc accatcgccg tggcgcggat
    21301 tacgatgccg caaagttatg tccggctgtc ggcagggcgc agcaatatgc ctgaagcaat
    21361 gcaggcgatg tgctttatgg cgggcgcgaa ctcgattttt tacggcgaca agctgctgac
    21421 cacgggcaat cctgatgagg acggcgacag aatcctgatg gaaaagctca acctgtatcc
    21481 cttgcagttt gaaccggaag gcgaggtcgc cgaagtggaa aaagcctctg ggattaaagt
    21541 ggattattga cgattgaaaa atgccgtctg aaacccggaa aaaggctttc agacggcatt
    21601 tgtccggacg gcatttccaa tatcttttta ccggcgcgtg atgctgccgt cgggcgagac
    21661 atccagcccg ttccccttgg ggaaatcgct gcggttcaga taaataatcc gcccgataac
    21721 ggttttctcg gggtcgattt tggggatttt atcgtcggct tgggtaatag ggatgatagt
    21781 tccggaaata aatttaccct gtttgtcggt gataattttg aaaatcggcg caatgccgct
    21841 gatgccggag gtgttgattg cgccgtaggt ggcaaagttg ccgccgctgt aggagatgaa
    21901 gcggtcgcgg taaagttcga cggcgcgtgt aacgtgcggc ccctgcccga atacgacatc
    21961 cgcgccggaa tcgacggcaa gccgtgcaaa ctcgacgacg ttgcccctgt tttccccata
    22021 gaagatttcg gtatcgaacg gcaggtgttc cgcctgtttc ccttccgcgc cgccgtggaa
    22081 catcacaata acgatctcgg ctttttgttt ggttttggca atcagttttt tgactttggc
    22141 atagtcgttc agtttgacgg cggcaaggtt gggggcgaag gagacgaagc cggatcttac
    22201 gccgtttttc ttcaggatgg cggtttcaaa cctgttttcg atgcccgaat atttgatgtt
    22261 caattcgtca aggttcgccc tttatgccgt ttccgcaccg caaaccgccg gggtcaagcc
    22321 ctcggcgcat cctgccggaa cggaatcccc gtgctgccga ttgacggttc aaaccccgcg
    22381 cccgtttcaa ataccggcga tgtggacgga caggatgcgc ctgacgaaaa gacagccgat
    22441 accgtttcca ttatcggcgt gggcgacatt atgctcggca gcaattatcc ggtcgattac
    22501 ctgcccgata ccaatattct gaaaaacgtc gaatctgcct tgcaagacgc ggacattacc
    22561 gtcggcaacc tcgaaggcac gctgtttgac gaaggcggta cgccgaaaaa atgtgcaacc
    22621 ccccaaaata tgctatgcat tccgaacgcc ctccgcatac gggcaatacc ttgccgacgc
    22681 gggattcgac tacctcagct tcgccaacaa ccacagcaac gacttcggcg cgcaaggcat
    22741 cacggcaacg gcggcgagcg gcagctcttt tacatactcg atcgcgctaa agccgctgcc
    22801 gataacgagg ccaaaattgc ggaaaatacc gccatcgccc agataaattt gtccatcatc
    22861 agacctttac tgttcagacg agacagcatt tgccgcacgt tttggggctt atctttcgat
    22921 ttgcgctacg tcgcgcaccg cgcctttgtc ggcggaagtc gccatcgcgc cgtaagctct
    22981 taatgcggcg gagacgtagc ggtcgcggtt ttccggtttc cacgctttgc tgccgcgtgc
    23041 ttccatttcg gcacggcggg cggcgagttc ttcgtcggaa atgacaaggc ggatgctgcg
    23101 gttggggatg tcgatttcga cggtatcgcc ttcgtgtacc aaaccgatcg cgccaccttc
    23161 cgccgcttcg ggcgaggcgt gtccgatgga caaacctgat gtgccgccgg agaagcgtcc
    23221 gtcggttaag agagcgcagg ctttgccgag gcctttagat ttcaggtagg aagtcggata
    23281 cagcatttcc tgcatgcccg ggccgccttt cgggccttcg tagcggatga tgacgatgtc
    23341 gccggcgacg atttggttgc ccaaaatgcc ttctactgcg tcttcttggc tttcaaacac
    23401 gcgggcgcgg ccggtgaatt tgaggatgct ctcgtccacg cctgcggttt ttaccacgca
    23461 gccgcgctcg gcgatgttgc cgaacaagac cgccaaaccg ccgtcttgcg agtaggcgtg
    23521 tgccacgtcg cggatacagc ctttttcgcg gtcgaggtcg agggttttcc acatacggtt
    23581 ttgcgagaac gcttgggtgg tgcgtacgcc gccgggcgcg gctttgaagc gttcgatggc
    23641 gtgggtgttt tcagggttgg ttacgtccca tttttcaatc gcgtctttca gcgtcggcgc
    23701 gtggatagtg tgcacgtcgg tgtgcagctt gcccgctttg tccaattctt tcaggatggc
    23761 gaagatgccg ccggctcggt gcacgtcttc catatagtag tcgtggttgt tgggtgcggt
    23821 tttgcagatg cagggcacaa cgcggcttaa gcggtcgatg tctgccattt tgaaatccac
    23881 gcccgcttcg ttggcaacgg cgagtaaatg caaaatagtg ttggtgctgc cgcccatggc
    23941 aatgtccatg gtcatagcgt tttcaaacgc ttttttggtg gcaatgctgc gcggtaacac
    24001 ggtttcatcg ttttgctcgt aatagcgttt ggtgatttcg acaatcatac ggccggcttc
    24061 gaggaacagt tctttgcggc cgacgtgggt ggcgaggtaa gaaccgttgc ccggcaggga
    24121 cagcccgagt gcttcggtca ggcagttcat cgagtttgcc gtaaacatac ccgaacacga
    24181 gccgcaggtc gggcaggcgt tttgttcgac ttcttcaact tgccggttgc tgatattgtc
    24241 gtccgccgat tcaatcatcg cgtcaatcaa gtccaaacgg cgttcgggct ggatgtttgc
    24301 cacgccgata actttacctg cttccatcgg accgccggaa acgaagatgg tcgggatgtt
    24361 caggcgcatc gcggcaatca gcatgcccgg ggtgattttg tcgcagttgg aaatgcacac
    24421 cagcgcgtcg gcgcagtggg cattgaccat atattcgatg gagtcggcaa tcaaatcgcg
    24481 gctgggcagg gaatacagca taccgctgtg tcccatagcg atgccgtcgt cgatggcgat
    24541 ggtgttgaat tctttggcga tggcgccggc tttttcgatt tcgcgggcaa ccagctggcc
    24601 catattgtgc aggtggacat gacctggcac gaattgggtg aacgagttgg caacggcgat
    24661 aatcggtttg ccgaagtcgg tttccatcac gccggtggcg cgccacaacg cgcgcgcgcc
    24721 cgccatattg cgaccgtggg tggaggtttt ggagcggtat tctggcatag tgtgtttcct
    24781 tgtgcctata ccgtctgaaa gacagggctg tttcagacgg tatcgggtac ggttttttag
    24841 agtgggaaaa gagggtattt tataccaagt atcggaattt tgcgggattg aaacggcgtg
    24901 cggcaaaaaa gaaaatcccc gcaggaatgc ggggacgggt tcaggcgcgg gcaatcgcga
    24961 cggctttgga tgcgtcccaa aaatcaacgg gtgcatttaa tacgggtttg acgatgcccg
    25021 tccgtatggc gaaatcgccg aaaccttcgc cgatattgcg ttctgccgcc catttgccga
    25081 tcaggtcgtc caattcggca aggatttcgg gcagggtgat gttttctttg taaagacggg
    25141 ggatgcgtac gccttcacgg tcgccgccga tatggaggtt gtagcgtccg acggctttgc
    25201 cgaccagtcc gatttccgcc aacatcgccc gtccgcagcc gttcgggcag ccggtaatgc
    25261 gggtaacgat gtagtcgtcc gacgtgccgt gtttcgccat aatcttatcc agctcgccga
    25321 tgaagtccgg cagcacgcgt tcggcttccg ccattgccag cgggcaggtc gggaaggaaa
    25381 cgcacgacat cgcattttcg cgcagtttgc tgacaccgtc gtgtatcagc ccgtatgagc
    25441 gggcgagctg ttcgatccgg gctttgcctc cttcgggtac atttgccacg atgaggtttt
    25501 ggttggcggt gatgcggaag tcgcctttgt ggattttggc gatttccaac acgccggtca
    25561 gaagctgttt cccgccttcg tcaaccaaac gcccgctttc gatgaaaagg gtcaaatgcc
    25621 agttgccgtc tatgcctttc acccagccga tacggtcgcc gcgcccggta aatttgaacg
    25681 ggcgtacggg ttcgaacggc atacccatac ggcgttcgac ttcggcgcgg aagttgtcca
    25741 agcccatatt ttgaatggtg tagcgggtgc gggcgttttt gcggtcgctg cggttgccga
    25801 agtcgcgctg cgtggttacc accgcttcgg cggccttcag cgcgtgttcc ggaggcacga
    25861 aacccagttc cagtgaaatg ttcggatagg ttttggtgtt gccgtgttcc atcgaaagcc
    25921 cgccgcctgc caaaacattg aagccggcaa gctgtccgtt accgtctgaa acggcgacga
    25981 aatccaaatc gttgccgtag cagtccacat cgttcaaggg cgggatgacg actgcggttt
    26041 tgaattttcg cggcagatag gttttgccca aaatcggctc gtcttcttga aggaagtcgt
    26101 cggaactttg aacttttttg ccgtccaccc acacatccag ataaccgcgc gtgcgtggca
    26161 ataggtgttc ggaaattttt tttgcgtatt cgtaagcctg ttggtggagt gccgactcga
    26221 tgggattgga ggtgcaaagc acgttgcggt tcatatccgc cgccgtggcg atggaatcca
    26281 aacccagttt gtgcaagagg cggtgcatcg tctgcaactt ggttttcggc acgccgtgaa
    26341 attggaaggt ttgccggttg gtcagccgga tggagcggta atgactgttt tcccgggcaa
    26401 atttgtccag ttctatccat tgggacggtt tgatgatccc gcccggcagt cggcagcgca
    26461 aaagcatgaa tttcaagggc tcgagttttg cctcggcgcg ttcggcgcgg atgtcgcggt
    26521 cgtcctgctc atacataccg tggaagcgga tgagttggaa gttgtcgcct ttgaagccgc
    26581 ccgtgagcgg gtctttcaaa tcgtccaaaa tcgtgccgcg taaaaaattg ctttcggttt
    26641 tcagacgttc gttgtcggat agcggttttt cttgccacgc caaacctttt gtcttggtct
    26701 gtacggtcat tttgtgttcc tcccgattat atttaatcaa taaacatcac gctgatagcg
    26761 tttttcttcg cgcagcatat ccaaatattc ttctgcgccc tcttcgtcca aatgtcctgc
    26821 cccgataatc acatccagca aggcggcttc cacgtctttt gccatttttg ccgcatcgcc
    26881 gcacacatag atatgcgcgc cttcctgcag ccattgccaa agtccttccg cctgttcgcg
    26941 gattttgtcc tgcacataga ttttttcttc ctgatcgcgg gaccaggcga aatcgtacct
    27001 gtgcaggaag ccgtctttgg caaactgctg ccattcggtc tgatagagga agtcggcggc
    27061 aaaatgcgga ttgccgaaaa tcagccagtt tttgccttcc gcattttctg cggcacgttg
    27121 ttggacgaag gcgcggaacg gtgcgacgcc ggtgcccgag ccgatcatca caatcggctt
    27181 gcggctgtct tcgggcagcc tgaagccgtc gttgcgttcc acaaacacgc gcaccgtgcc
    27241 gtcctcttcc agccggtcgg caaggaaacc cgatgcgccg cccgccctgg cgcgcccttc
    27301 gtgttcgaaa cgcaccgcgc cgacggtcag gtgcacttcg tcccccactt ccgcctgcga
    27361 cgaggaaatc gaatacaggc gcggcgcaag cgggcgcagt aggccggcga attgttccgc
    27421 cgtcagtttt gccgggaagc ggtgcagcac atcggcaatc ggcgtgcttt gcacaaagcc
    27481 ttgcaaaacg gcgttgtcgg cagcaatacg gtcgagttcg tcatcatcgg cgaacggggc
    27541 atagcctttg acaaaggcgg gggtgttttg cgtgagttca aaatgggata acagtgcgga
    27601 ggcaaccggc agggtttttc cgcccgcctg tatttccgtt gccggatcga tgccgagcag
    27661 gtctaggatt tccctgacca gtgccggatc gttgtcaaac caaacgccga gcgcgtcgcc
    27721 cggtaggtag tgcaaatccg aaccgctcaa atcgatttcg atgtggcgca cgtctttatc
    27781 ggattggcgg gcggtaattt tctgattggc cagcagggcg gcgggaaagg gggctgcctt
    27841 gcagtacctg ccctccgatg ccgtctgaag gccggcgggg ggcgttgcct gcggcgcggg
    27901 cgttgcccgg ttttttgcgg cttcttcttt taagagtgcg gcgatattgt ctgtccaggc
    27961 gtttgcggcg gcggcaaagt ccaaatccgc atcaacgcgt tcgagcaggc gtttcgcccc
    28021 caattcttca aaacgtttgt cgaaatcttt gcccgcccgg cagaaattcg gataggaact
    28081 gtcgcccaaa cccagtacgg caaattggag tttgtccaat ttcggggctt ttttgccgtt
    28141 cagcagtttg tgcagcacga cggcttcttc cggcggttcg ccttcgcctt gggtggacgt
    28201 aaccagcagc aggcggcgtt cgccggcgat gtttttcgcc ttatagtctt tcagttcggc
    28261 gcgactgact tggatgccgg cggcttccag gctgtccgcc gctttgtcgg caacggattt
    28321 cgcattgccg gtttgcgagg cggaaaggac ggttacggaa aaaggttctg ccgtcggcaa
    28381 tgccgtctga agcgcgggca gtcctgcaga tgccccgttt cctgcttttg cccaagcgta
    28441 gccggacagc cacgcccatt gtgccgcgtt cagccccgac aggagctgcg tgatttcggg
    28501 cggcataggc ggtaatggcg gatttgtgtt ctgcatatcg tgttcactca taaaatcata
    28561 cctgccgcaa cagtgccgta tgtcgcttcg tctatcagga taaacgaacc ggcggcggtg
    28621 ttttccgcat aaggcgttgc cgtaacgggt ttttgaaggt tgatgcggac tttggcgatg
    28681 tcgttcatct tcaaggattc cgcgccggcc tcttgttcca gcgtgcggac atccaaaacg
    28741 ctttcaattt ccccgacttt tgccggcacg gtttgcgtgc cgtgcttgag caggtatttg
    28801 cgcgcggtgt tgagcggacg ttcgtcaaac cagcaaagcg tggcttccag atgtttttgc
    28861 ggggcgagcg gggaattttt atcgacaaaa aggtcgccgc gcgaaacatc gatgtcgcga
    28921 tccagccgga gggttgccgc ctcgccggca aaagcctgcg ccacttcccc tttcggcgtg
    28981 atgatttcgg acacttcggc ggtcagcccg ttcggttcga tgcggacggt ttgcccgacg
    29041 gcgaccgaac cgcgttcgat gcgcccctga tagcctcgga aatcatcggc cttgtcggca
    29101 tcttggcgga cgaccagttg cacggggaaa taaaaatcgg cagcggaacg accggtttcg
    29161 tccgcccccg gcaggttttc caagatggcc agcagcggtt cgccccgata ccacggcata
    29221 ttgccgccgg ggtaaacgag gttgtcgccc aagagtgcgg acaccggtac gaaatgcacg
    29281 tctttcaaac cgagccgtcc ggcgagccgg cggtatgcct ccacaatggc gttgaatttg
    29341 tcttcgctgt aatccagcag gtccattttg ttgaccgcca ccacaatatg cgggcagtcg
    29401 agttggcgga ggatggcgga atggcgtttg gtctgcggca gaagctgcaa gggctgcgcg
    29461 ccgaaatcca gttgggatgc gtcaaccagc acgactgccg ccgaagcggt gcttgcgccc
    29521 gtaaccatat tgcgcgtgta ttgttcgtgc cccggcgtgt cggcgatgat gaatttccgt
    29581 ttcgccgtgg aaaaatagcg gtatgccaca tcgatcgtaa tgccctgttc gcgttcggct
    29641 tccagtccgt cggtcaggat ggagaagtct atggtttctt tcaaaccttt gcttttgccg
    29701 gattccaagg ttttgatttg gtcggacagc agggctttgc tgtcgtagag cagtcgtccg
    29761 attagggtgc ttttgccgtc atcgacgctg ccggcggtaa tgaagcggag cggggtttgg
    29821 tgttgtgccg tcatattttc ttcctcatat ctgcttaaag ggtttttgaa atttagaaat
    29881 agccttcttt tttgcgtttt tccattgccg cctcgcttgc ctgatcgtcc agccgggtcg
    29941 cgctgcgttc ggaaatgtcg gcaaccgctg tttctctgat aatctccgtc ggcgtggacg
    30001 cggtgctttc taccgggcag gtgcagctga tgtcgccgac ggtgcggaag cggacatcaa
    30061 ggatttcgga ggtttcagac ggcattttcg gggtgagcgg cgttacgggg accagcagcc
    30121 ccctgcgtct gaccacttcg cgcctgtggc tgtaataaat cggcggcagc tcgaggtttt
    30181 cgcgggcgat gtattgccag atgtcgagtt ccgtccagtt ggaaatcggg aagacgcgca
    30241 tattttcgcc tttgtgcagc ctggtgttgt acagcgacca cagctcgggg cgttgcgcct
    30301 tcggatccca ttgtccgaac tcgtcgcgga acgagaaaat ccgttctttg gcgcgggctt
    30361 tttcttcgtc gcgccgcgcg ccgcccataa gcgcgtcgaa gccgtttgcc tcgatggttt
    30421 ccaacaaggt aaccgcctgt gccgcattgc gcgaatcggt ttctttgcgt aagaccaccg
    30481 tgcctttggc aatggagtct tccacgcgcc ccactatcag gcgggcattg agttttgccg
    30541 cctgcgcgtc gcggaaagca atcacttcgg ggtagttgtg tcccgtgtcg atatgcacca
    30601 gcgggaaggg cagtttcacc ggccggctgc ccagccggaa ggctttgcag gcgagggcga
    30661 gcaggaccac ggaatctttg ccgccggaaa agagcagggc ggggttttcg cattctgccg
    30721 ccacttcgcg gataatgtgg atggattcgg attccaacca gtcgagttgg acgttgttcg
    30781 gttcggtttt cgtcatacca tattccttat ttcttctgtt tgatccttat gaattatttg
    30841 tgcagcccgc attctttgct gtttttatct tcccaccacc accgcccggc gcggatgtct
    30901 tcgccggcct tgacggggcg ggtgcagggg tcgcagccta tactgggaaa gccttgccgg
    30961 tacaaatcgt tgtaaggcac attgttggca aggatgtatg cccacacgtc gtgttccgac
    31021 cagtcgaaaa tcgggttgta tttatcgatg ccgcgcccgg catcatattc ggcaaacggc
    31081 agttccgtgc gtgtggcgga ctgttcgcgg cgttgcccgg taagccaagc atccgccccg
    31141 gcaatggcgc ggtcgagcgg ttcggttttt cggatgcggc agcattcgcg gcgcgcttca
    31201 acgctgtcgt aaaaggcaaa cctgcctttg ctttccacat aatgcagggc atcttcccgt
    31261 atcggttgga aacgcttgat ttgcatatgc ggataaacac gttcaatcct atccaacagg
    31321 tttaaagttt ccgcgtgcag cagccccgta tccaaagtaa aaataccgat attgaggttt
    31381 tctccggcga taaggtcggt aattaccata tcttccgcag caaggctgct ggcaaaccgt
    31441 gcatcccggt ggctgccggc aatccggtgc aggcgttgtt tgagggtttc cgttttttcc
    31501 gccaaagccg tttcactgcc attttcaatc ggcggaattt tccataaagc aggtttgaaa
    31561 attttcattt tgtccgcctt gctttatgcg gcttgttgtt cttgtccggt gcccagtccg
    31621 tccaatgcaa aatacgcgtg ttcggccgga ttgctttctt ttttggcgtt ttcgccgaac
    31681 caagccagtt tttcgtgcag ccccaccact tcgcctatga caatcaatgc cggattcggc
    31741 gcggtttcgg cgagttcggc aaggttggcg agcgtgccgg ttgcggtttt ttgagccggc
    31801 agcgtgcctt ggctgatgac ggctgccggc gtgtcgggcg agcgtccgtg ctgttgcagc
    31861 cgttcggcaa tcagggcggc tttgagcgca cccatataaa tcaccaaggt ctggcggctg
    31921 cgggcaaggg tctgccattc gatgtcgggc gcatccgcct tgcggtggcc ggttacgaaa
    31981 accgcacttt gggcataatc gcggtgcgtg agcgggatgc cggcataggc ggtcgcgccg
    32041 acggcggcgg taatgccggg gacgaccgaa aacggaatct gatggcgtgc caaggtttcc
    32101 aattcttcgc cgccgcgtcc gaacacgaaa gggtctccgc ctttcaaacg caccacgcgc
    32161 ctgccttcgc gggccagcct gaccataagc gcattggtgt cctcttgcgg ggtgcggccg
    32221 ccccgggcgc gcttgccgac aaaaatccgt tccgcatcgc ggcggacgag ggacagtatg
    32281 ccgtctgaaa ccagcgcgtc gtaaagcacc acgtctgcct gctggatttc ctgcagccct
    32341 ttgagcgtca gcagccccgc atcgccggga cccgcgccga ccagcgagac ggagccgcct
    32401 tgatcatttt gacgactttg ttccaattgg cctgccaatt cccgttcggc aagggtgttt
    32461 tgccggtttt tgacgagggc ggcgaaacgt ccgttaaact gcttttccca aaagcggcgg
    32521 cgttcggtaa cggatttcag tttgcccttg acggcatcgc gccaccttcc tgaaatttcc
    32581 gccatatcgc ccaaagacgg cggcagcagg gcttccagcc tttcacgcag cagtcgggcg
    32641 aggacgggcg cgctgccgga gctggaaacg gcaatctgaa ccgggttgcg gtcgataacc
    32701 gatgggaaga tgaagctgca atggtcgcgg tcatccacca cgttgaccgg cttttggcag
    32761 ctttcggcaa gatggaaaac gcgccggttg agggcttggt cgctgcttgc cgcaatgatg
    32821 aggaaaaccg tgcggatgtg ttcggcacga aattcttcgg caagccacag gattttgttt
    32881 tccgccgcca acgcggagag ttcggcattc aggtgttttg cggcaaccct gacctctgcg
    32941 cccgccttca gcagcaggct gattttgcgt gcggcgactg cgccgccgcc tacgaccaat
    33001 acggggcggc cggcgaggtt ggcgaaaatg gggaaataat tcactggctg actcctttgc
    33061 tgtttgcccg caccttgttg ccgatacggt gcgtcgcggc atttttgtcg gaatgcgggt
    33121 cattttagac aaaaggattt tcccggttaa ataataaaaa ggcatttgtt agaagctgaa
    33181 agctatatgg gggcggctgc ggatgcggcg gttttccgtt ttataacggt ttgggacgga
    33241 aaaacggcct gaagccgttt cgggcattca gaccgtttgc gcggaggggg aatgccgtcc
    33301 gaagggcgaa aagggcttca gacggcattg atgtcgggtt tcaggacagg agcaggatgg
    33361 cggctgcggc aagcgcggca accgataatg cggcggcaag cgcggctttg ccggcaaagc
    33421 ggattgaggt tttgccttcg atgtatttga agccggttat catcgggagg acgaggtttt
    33481 tctttttgaa cacgcggtat gcggcgacgg cggcgatgtg gactgcggaa aaaacggcga
    33541 gcagtttgaa aaagttgaga tggattttcc gcataaggct gcccgtatgc tcggaaacca
    33601 aatggttgag gtagccgttg gtgctgaagg tgttttcatc ggcggcaaaa agccctgtgc
    33661 cgacttgaaa tgagacggcg gccaaaagcg cgacgaccat cagtgcgccc aaggggttgt
    33721 gtccgggttg gacgtgttcg ggaataccgt ttttcatata ctctctgata cccgaccatc
    33781 cgcggacgaa acgggagaaa cgggcggtat cgctgcccca aatgccccag cagaggcgga
    33841 atacgagcag gaaaaggata aacagcccga cgcgcgtgtg ccattgcagc atatcgccgc
    33901 cggttttcgc gctataccac ataaagggta gggatgcggc aagcagccaa tggaaaaggc
    33961 gggtggggaa gtcccagact ttggttttgt ttttcataat cggtttccgg cggtagaatc
    34021 ggtttgtttt cgagccttaa ttttaaccga ttggaggggc aatgtttccc gtttttcatc
    34081 tttcaggcga gagccgccgc cggatgcttc agacggcatt gcgttttccc tatgttttca
    34141 aagcccgtgc ggaagattcg cacaaaggga ctttcggcac gctcgccgta gtcggcggat
    34201 cggcagggat gagcggtgcg cccgtattgg cggcatcggc ggcgatgtat ctcggctgcg
    34261 gcaaggtgtg ggcgggtttc aatcaggatg cgctaccttt tgccgttatt gccggttttc
    34321 ccgagattat gctggatacg gcagacggtt tgagcaagcg tcaaggcata aacgcttgga
    34381 cggcgggttg cggcttgggt acggataagg cggcggtcgg aacgcttgcc ggaattttga
    34441 cggaacacac ggacaagccc gtccttttgg atgcggacgc gctgaacata ttatcaaccg
    34501 atgccgaaac ccgaaatctg gcacgcgggt gtaaaaacct gattttaacg ccacaccccg
    34561 ccgaagccgc gcgcctgctt ggaacgacgg ttgcacaggt tcaggcggat cggacggcgg
    34621 cagtgaggaa gataggggca attttcggcg caaccgtggt tttaaagggg cacaaaacat
    34681 tggttgcctc acccgatacg gaaatctatg tcaacgaaag cggcaacgcg ggattggcaa
    34741 cggcgggcag tggcgacgta ttgggcggca tcatcggcag tctgctcgca cagggcgtgc
    34801 cggtttttga agccgcctgc gcgggcgcgt ggctgcacgg cgcggcggcg gatgtcataa
    34861 aagaatcggc aggcattgcg gcagggctgt tggctggcga aatcgccccg gcggcaaggt
    34921 ggctgcgcaa ccggataact gaaagtatgt aagaagatat agtggattaa caaaaaccag
    34981 tac
//
LOCUS       DAWFIT010000010        33303 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFIT010000010 DAWFIT010000000
VERSION     DAWFIT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.010"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(109..1032)
                     /gene="era"
                     /locus_tag="WGQ85_000459"
     CDS             complement(109..1032)
                     /gene="era"
                     /locus_tag="WGQ85_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0749958.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1079..1798)
                     /gene="rnc"
                     /locus_tag="WGQ85_000460"
     CDS             complement(1079..1798)
                     /gene="rnc"
                     /locus_tag="WGQ85_000460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0749959.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1965..2288
                     /locus_tag="WGQ85_000461"
     CDS             1965..2288
                     /locus_tag="WGQ85_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0749960.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            2335..2811
                     /locus_tag="WGQ85_000462"
     CDS             2335..2811
                     /locus_tag="WGQ85_000462"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0749961.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2889..3314
                     /gene="nusB"
                     /locus_tag="WGQ85_000463"
     CDS             2889..3314
                     /gene="nusB"
                     /locus_tag="WGQ85_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0749962.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGQ85_000464"
     CDS             complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGQ85_000464"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0749963.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHISTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4774..5379)
                     /locus_tag="WGQ85_000465"
     CDS             complement(4774..5379)
                     /locus_tag="WGQ85_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749964.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5380..5604)
                     /locus_tag="WGQ85_000466"
     CDS             complement(5380..5604)
                     /locus_tag="WGQ85_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0749965.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5713..6324
                     /locus_tag="WGQ85_000467"
     CDS             5713..6324
                     /locus_tag="WGQ85_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0749966.1"
                     /translation="MRRAILLILALTAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            complement(6384..7256)
                     /locus_tag="WGQ85_000468"
     CDS             complement(6384..7256)
                     /locus_tag="WGQ85_000468"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0749967.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7293..8078)
                     /locus_tag="WGQ85_000469"
     CDS             complement(7293..8078)
                     /locus_tag="WGQ85_000469"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0749968.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESV
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            complement(8505..8597)
                     /locus_tag="WGQ85_000470"
     tRNA            complement(8505..8597)
                     /locus_tag="WGQ85_000470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8561..8563),aa:Ser,seq:gct)
     gene            complement(8754..8951)
                     /locus_tag="WGQ85_000471"
     CDS             complement(8754..8951)
                     /locus_tag="WGQ85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749969.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            complement(9056..9373)
                     /locus_tag="WGQ85_000472"
                     /pseudo
     CDS             complement(9056..9373)
                     /locus_tag="WGQ85_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9396..10157)
                     /gene="kdsB"
                     /locus_tag="WGQ85_000473"
     CDS             complement(9396..10157)
                     /gene="kdsB"
                     /locus_tag="WGQ85_000473"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0749970.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            complement(10154..10336)
                     /locus_tag="WGQ85_000474"
     CDS             complement(10154..10336)
                     /locus_tag="WGQ85_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0749971.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            complement(10405..10986)
                     /locus_tag="WGQ85_000475"
     CDS             complement(10405..10986)
                     /locus_tag="WGQ85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0749972.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(11192..12226)
                     /locus_tag="WGQ85_000476"
     CDS             complement(11192..12226)
                     /locus_tag="WGQ85_000476"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0749973.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNEKSRAEDAGDEPLLLFRKTVAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(12266..12537)
                     /locus_tag="WGQ85_000477"
                     /pseudo
     CDS             complement(12266..12537)
                     /locus_tag="WGQ85_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12773..14053)
                     /locus_tag="WGQ85_000478"
     CDS             complement(12773..14053)
                     /locus_tag="WGQ85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0749974.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(14275..14895)
                     /locus_tag="WGQ85_000479"
     CDS             complement(14275..14895)
                     /locus_tag="WGQ85_000479"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0749975.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            complement(14955..15950)
                     /gene="mltG"
                     /locus_tag="WGQ85_000480"
     CDS             complement(14955..15950)
                     /gene="mltG"
                     /locus_tag="WGQ85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0749976.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            16034..16606
                     /gene="ampD"
                     /locus_tag="WGQ85_000481"
     CDS             16034..16606
                     /gene="ampD"
                     /locus_tag="WGQ85_000481"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0749977.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPNGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDDMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            16800..18086
                     /locus_tag="WGQ85_000482"
     CDS             16800..18086
                     /locus_tag="WGQ85_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0749978.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            18234..20759
                     /gene="ligA"
                     /locus_tag="WGQ85_000483"
     CDS             18234..20759
                     /gene="ligA"
                     /locus_tag="WGQ85_000483"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0749979.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKEAADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            20792..21991
                     /locus_tag="WGQ85_000484"
     CDS             20792..21991
                     /locus_tag="WGQ85_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0749980.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            22210..22470
                     /locus_tag="WGQ85_000485"
     CDS             22210..22470
                     /locus_tag="WGQ85_000485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749981.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            22577..23101
                     /gene="nspA"
                     /locus_tag="WGQ85_000486"
     CDS             22577..23101
                     /gene="nspA"
                     /locus_tag="WGQ85_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0749982.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            23237..23626
                     /locus_tag="WGQ85_000487"
     CDS             23237..23626
                     /locus_tag="WGQ85_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0749983.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFKNLQAVAEAAGGTLADIVKLNAYLTDLANFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            23704..24534
                     /locus_tag="WGQ85_000488"
     CDS             23704..24534
                     /locus_tag="WGQ85_000488"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0749984.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGYGEGAPKRSDTIEPILKHPDGRKMLDWL
                     RAQPLLIREGNRVMVHAGILPQWRITKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPLYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFTK
                     "
     gene            24549..26006
                     /locus_tag="WGQ85_000489"
     CDS             24549..26006
                     /locus_tag="WGQ85_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0749985.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILTAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(26047..26436)
                     /locus_tag="WGQ85_000490"
     CDS             complement(26047..26436)
                     /locus_tag="WGQ85_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749986.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(26584..26973)
                     /locus_tag="WGQ85_000491"
     CDS             complement(26584..26973)
                     /locus_tag="WGQ85_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749987.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIGDIDNIVDFVSSYSLPKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            complement(27095..27484)
                     /locus_tag="WGQ85_000492"
     CDS             complement(27095..27484)
                     /locus_tag="WGQ85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749988.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVEIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            complement(27585..27983)
                     /locus_tag="WGQ85_000493"
     CDS             complement(27585..27983)
                     /locus_tag="WGQ85_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749989.1"
                     /translation="MLGLTRDNCYDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQGFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            complement(27986..28177)
                     /locus_tag="WGQ85_000494"
     CDS             complement(27986..28177)
                     /locus_tag="WGQ85_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0749990.1"
                     /translation="MRLSDGIFDTKQVTKGVFDGKGEAKIPSYTWHHHQDTGRMQLIS
                     ERKHTQTGHIGGEAMDKGK"
     gene            complement(28238..28639)
                     /locus_tag="WGQ85_000495"
     CDS             complement(28238..28639)
                     /locus_tag="WGQ85_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749991.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEENNSDGNPTANYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(29056..29385)
                     /locus_tag="WGQ85_000496"
     CDS             complement(29056..29385)
                     /locus_tag="WGQ85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749992.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(29411..30118)
                     /locus_tag="WGQ85_000497"
                     /pseudo
     CDS             complement(29411..30118)
                     /locus_tag="WGQ85_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            30074..30217
                     /locus_tag="WGQ85_000498"
     CDS             30074..30217
                     /locus_tag="WGQ85_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749993.1"
                     /translation="MNMGNGFSAVCRFDQGAAVEGAGVGFLKNSVLNILLQMKERPSE
                     KQA"
     gene            complement(30253..30795)
                     /locus_tag="WGQ85_000499"
     CDS             complement(30253..30795)
                     /locus_tag="WGQ85_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0749994.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLNNEVDNLSDMKRKPFAW"
     gene            complement(30922..31149)
                     /locus_tag="WGQ85_000500"
     CDS             complement(30922..31149)
                     /locus_tag="WGQ85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749995.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(31208..31531)
                     /locus_tag="WGQ85_000501"
     CDS             complement(31208..31531)
                     /locus_tag="WGQ85_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0749996.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(31534..32241)
                     /locus_tag="WGQ85_000502"
     CDS             complement(31534..32241)
                     /locus_tag="WGQ85_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0749997.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            complement(32375..32692)
                     /locus_tag="WGQ85_000503"
     CDS             complement(32375..32692)
                     /locus_tag="WGQ85_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0749998.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(32695..>33303)
                     /locus_tag="WGQ85_000504"
     CDS             complement(32695..>33303)
                     /locus_tag="WGQ85_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233664.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0749999.1"
                     /translation="PAEGKFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVF
                     NVAAAAKVKNLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKS
                     IGTKIHDGAQGKHISGHGNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVV
                     DFGYPIGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         8195 a         9375 c         7591 g         8142 t
ORIGIN      
        1 tatgcacaga taatatcaac ccgtttttaa caaagatatt cccgattgaa acaaaatgcc
       61 gtctgaaacc tgttcggacg gcatttgcgt aaagttcagc aagaaaaact acaaacccag
      121 ctcgcgcagg aagcggatgt cgtccgccca accggatttg actttgaccc agaccttcaa
      181 aaatactttg gtatcaaaca gtttttccat atccaaccgc gcttcggtgg aaattttctt
      241 caaacgctcc ccgcctttgc cgattaaaat cgccttttgg ctttccttat cgaccaaaac
      301 ggcgatatag atgcggttca aaccgtcttc ctcttcaaac tgctccactt cgacgttcat
      361 cgcataaggc aattcctcgc ccaaatagcg gaacaatttt tcacgcacga tttccatcgc
      421 taaaaaacgc gccgatttgt ccgtaaccat atcttcggga tacatcggca cgctttcggg
      481 cagatacggc ttaatcagct ccaacaggtt ggcaatccgc aatccgtgtt tcgcgctgac
      541 cgcctccgcc gccgcaaatt caaattcggc gcgcacctgg gcaacaaacg cctccagcgc
      601 gtaacggtct ttcgccttgt ccttatcgat tttgttgacc actaaaatga ccggcgtgtg
      661 cttgggcagt tgtttcaaca cgacgcggtc ggcatcggta aaacgcatcg cttccacgac
      721 gaaaaccacc acatccacgc cgccgagtgc ctcggtaacg ttttgattca aacggtcgtt
      781 gagcgcgttg cggtggtcgg tttgaaaacc gggcgtatcg acaaacacaa actgcgcggt
      841 atcgtcggta taaatccccg ttacgcggtt gcgcgtcgtc tgcgcctttt tgctggtaat
      901 actgattttc tgaccgatga gatgattcat cagcgttgat ttgccgacat tcggacggcc
      961 gacaatcgct acgaagccgc aacggtatcc gtcggcggcg cgttcccctg caaggaaggt
     1021 ttcaatatcc atatttgctt tcgtatccat atttcagacg gcatattcac gcgccgcctc
     1081 atttcttttt cttcttcagc ggcagcttct cttccagcca tttcaaagcc tctttcgccg
     1141 cttcctgctc cgccgccttg cggctcgtcc ctttggcacg gcacacgaaa cccagttcgc
     1201 ccaaatcgca ggaaatgaca aacatactgt cgttggcatg gccgatttgc tcttcgatac
     1261 ggtatttcgg caaggcgaaa cggcgcgcct gcaacgcctc ctgcaaagca gttttgccgt
     1321 cttttgcctg attttggaaa tcgactcgcc ggacgcgttc tgcaaacaaa tggcgcacca
     1381 ccttttccgc cgtgttgaaa tccgcatcga agctgaccgc cgcaaacatc gcctccatcg
     1441 cgtccgccag tatcgaaggc cgtctgaagc cgccgctctt caactcgccc gcccccaagt
     1501 acaggccgtc gccgacattc atttccgccg ccatttccgc cagcacgccc tcattgacca
     1561 gacttgcccg caaccgcgac aactcgccct cggtcaactt cggaaacgcg tcaaacagca
     1621 tccgcgccac cgtataattc aaaatcgaat cgccgacaaa ctcgaaccgc tcgttgtgct
     1681 tcgcatgatg gctcctgtgc gtcaaagcct gccgcaaaag cgaaatatcg cggaacgcgt
     1741 agcccagttt cttctgtatc gccgcgtgtg cctgctgttt caaaacatcg tctttcatta
     1801 ccgtcttccg caaaaaacag cccgaatgcg tttccatact ccaaaaatac agaaacacct
     1861 taaggctgca aacggcgcac attgtaccac caaaccgccg ccgtctgccg acaacgcaag
     1921 aatcgtggta aaatccgcaa gttttccccc aacgtattcc cgcaatgaac gcatttgcac
     1981 aagcactttc ctcggcactc gaccgctgca tcgccaccaa caccgtcgcc aaacaaaacc
     2041 agcccgtcgg cttcctctac cgcgaagccc ccgtttttga aaacgacagc ggctggcgtt
     2101 tcttcagcgg cgacgagacc gacgaataca ccgacgaccc ggacaacttc agcatcgtca
     2161 gcctcgccga tatcaccaaa accaaccctg aaaccgccgc actgctctcc caacctgaag
     2221 gcagcgcgtg ggaattggca gaagacggca cattccaaac cgtcgccgac tggcaaccta
     2281 aggactgacc cttcagacgg cattccgaaa cctccgaata ccaaaggaca ctcaatgaac
     2341 accatcgccc ccaacctcga cggcaaacac ctccgcatcg gcatcgtaca ggcacgcttc
     2401 accaacgaaa tcggcagcga aatgctcaaa gtctgctgcc gcaccctcca agaattgggc
     2461 gtggcagacg aaaacattac cgtcgccacc gtacccggcg cgcttgaaat ccccatcgcg
     2521 ctgatgaact ttgcctcttc tgaaaaattt gacgcactga ttgccatcgg cgtcgttatc
     2581 cgtggcgaaa cctaccattt cgagctggtt tccaacgaat ccggagcagg ggtcagccgc
     2641 gtcgcactcg actacaacat cccgattgcc aatgccgtcc tgaccacgga aaacgacgca
     2701 caggcaatcg aacggattga agaaaaagcc tcggatgccg ccaaagtcgc cgtagaatgc
     2761 gccaacctcg tcaacctcct gctcgaagaa cagtttgaag acgaagaata acaaacaggg
     2821 cgtacaccga tacgcccttt ccgtttcaga cggcataccg tgccgcccaa ccgtttcaag
     2881 gaaaaatcat gaaaacagcc cgccgccgtt cccgcgagct tgccgtacaa gccgtttacc
     2941 aatcccttat caaccgcacc gccgcgcccg agattgctaa aaacatccgc gaaatgcccg
     3001 actttgccaa ggcagacgaa gaattgttca acaaactttt cttcggcacg caaaccaatg
     3061 cggcagagta catccgacaa atccgccccc tgctcgaccg cgacgaaaaa gacctcaacc
     3121 ccatcgaacg cgccgtcctg ctgaccgcct gccacgagct gtccgccatg cccgaaacgc
     3181 cctaccccgt catcatcaac gaagccatcg aagtaaccaa aaccttcggc ggcacggacg
     3241 ggcacaaatt cgtcaacggc atcctcgaca aactcgccgc ccaaatccgt cccgacgagc
     3301 ccaaacgccg ttgatgcatc acaaatttaa agaaagttct atgtccttat ttctgctttc
     3361 tttgtttcgt cattcccacg caggtgggaa tttggttttt tgaattttgg gtatttcaaa
     3421 taaattctga aacttcaatc tcgtcattcc cgcgcaggcg ggaatccggt tttttgagtt
     3481 gctgccgttt ttgataaact ctggtcgcat cgaatttccg gattcctgcc ttcgtgggaa
     3541 tggcgaaatt tgaaaattgt gggaatttat cggaaataac cgaaacgtaa caaactcaaa
     3601 cgccgttgac gtatcaatca gtcaacaaca caaatgccgt ctgaaaaacc tttcagacgg
     3661 cattttgttt ccagccccat caatactgta ccgtccagcc gatttcgccg cccgcgcgca
     3721 tcgggacaag ttcgccgtcg ccgtagggaa tacttgcggg gacggtttgg ctttgtttga
     3781 cgagggtgat cgtgtcggta ttttcaggaa tgccgtaaaa ccttgcgcca tttttcgagg
     3841 caaaggcttc gagtttgtcc aacgcgcctg ctttttcaaa cacttcggcg taaagctcga
     3901 cggcggtcat cgcgctgaac ataccggcgc aaccgcaggc gttttctttg gcggatttgg
     3961 cgtgcggcgc ggagtcggtg ccgaggaaga atttatgcgc cttctcgccg gtaacggcgg
     4021 caaccaatgc ctgacggtgg gtttcgcgtt tgagtacggg caggcagaaa tgatgggggc
     4081 gcacgccgcc gaccaagagg tcgttgcggt tgagcaggag gtgttgcggg gtcacggagg
     4141 cggcaacgtt gtcgcccgct tccaaaacca ggcgggcggc ttcggcggtg ctgatgtgtt
     4201 cgaacacgac tttaagattc ggcacttgcg ccaaaacggg tttcatcacg cgcccgataa
     4261 aggcggcttc gcggtcgaag atgtcgattt cggggtcggt tacttcgccg tgaacgagga
     4321 acaaaatgcc ctgtttcgcc atttcttcca acacggggat gagcttgaac aggtcggtta
     4381 cgccggaatc ggaattggtg gtcgcgccgg cagggtagag tttgaaggcg acgatgccgg
     4441 cggctttggc ttcgcgcaca agtgcaggcg tggcttggtc ggtcaggtaa agcgtcatca
     4501 acggctcaaa cgcgctgcct tcgggcaatg ccgccatgat gcgcgctttg taggcaagcg
     4561 cgtcggcaac gctgacgaca ggcggtttga ggttgggcat aatgacggca cgccccattt
     4621 ggcgggcggt ataaggagca acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca
     4681 tatcgtcggg gcggataatg gtcagggttt gcatggtttt cctttgtttt atttgaatgt
     4741 tcagacggca tgccgacttt atgccgtctg aagttagggg agaattaggg gtttcccgtt
     4801 gtgggaacgg tgcgtcaggt tcaaaactgc ctgtacgggt aaatccgggg aatcggcggt
     4861 ttgtgcggtc aatacggttt cttcggagct gcccgcttcc cgccaggcat agctgacttg
     4921 gcggatttca aatgatgcct gcaccgactg ttccgccctc aattctatat tggcattttt
     4981 cggcggctgt cggacaagtc cgtcaaaatg ttccaacagc gcggcactgt ctgcccgctc
     5041 ggctttcaca ctgcaactgt cgcgcgtcag gctcaaataa taaatgccgc catcgcgttc
     5101 aagcgtccac aacgcctgta catcgggttc tgcaacagtg ggcggcagat gggacaggtc
     5161 ttcctttccg acagggacaa aatgtccgac agcggcaaac ttggaaaccg attcaaaact
     5221 gccttgagtc aggacgcagg cactccgaaa caaatcgacg gctacacgcg cgaaatcctg
     5281 atgccgtaca ccggattgcc ggttttgggc attacacgca gcaagcacaa atgccgacag
     5341 caataacatc aatcgggaca tccgcatttc agacggcatt tatttgtgtt ggacgaccag
     5401 cgtgaacacg ccgtcctgtt cggaagcatc caacagcaca tgaccggttt ggcggcaaaa
     5461 agcctcaaaa tcccccggtg cgccgccgtc ggttgccaga acggtcaaca cgtcgccctg
     5521 ctgcatttgc gccaaagcct ttttcgcccg caaaatcggc aggggacatt tcaatccggt
     5581 tacgtctaaa gtttcgctgt tcatcgtttg taccgtttaa aaaatgggaa tcggggggag
     5641 attatacccc agttgcttgc agcccccttt accaacccat aaaataacgc cgttttcaat
     5701 ttggagagat ctatgcgccg cgccatcctt ctgattctgg ccctgactgc cggcacgtcc
     5761 cttgccacca gcttcagcca aaaagacacg ccgatcaaca cccgctaccg cgaaacgccc
     5821 gaagaagccg ccgcccgcga atttaaagaa cacaccgcca aactgccgcc gctgcccgat
     5881 acgcactcgg acggctggtt tgacatttat gtggatgaaa attacggcaa acagccgaaa
     5941 atcctgctcg acagcctgca aattatgccc gcgcccgacg gcagcatccg ctacattctc
     6001 aatatccgct ccgacaaagg ctacgacaac ctgactgccg aaggcatatt ctgcgcccgc
     6061 ttctccatca ggttcggcaa cgacaaactt tcctcctaca aagtgttcgg ctacggcgac
     6121 acggtcaacc gccgctggat acagccccga aacgccgaat ggaaaccgat aggcggtaca
     6181 ttaagccgca acgacgcact gcgcaccgtc ctgtatcagg cattctgcga gggcggtgta
     6241 cccgccgaca cgaaaggttt ggtacaacgc ctgaaagaac gtgccggacg ctatgccccg
     6301 tcgataaagc cgcacgacaa ataaccagac acaaaaatac cgtctgaaac atcggtttca
     6361 gacggtattc gattcatcag ccatcaggcg gcagaaactt tgtcctgacg gcacaacagc
     6421 gtaatcaaat cactgatgcg ccgcttcata tcgcggcggt cgacaatctg gtcgattgcg
     6481 cctttttcca gcaggaactc ggcgcgttgg aagccttccg gcagcgtttc gcgcaccgtc
     6541 tgctcaatca cgcgcggacc ggcaaaaccg atcagcgcgt tcggttcggc aagcacaaca
     6601 tcacccaaaa atgcgaagct ggcggatacg ccgcccatag tcggatcggt caacaccgat
     6661 ataaacggca ggcgtttttc cgtcagcaaa tgcagcgcgg cactggtttt cgtcatctgc
     6721 atcaatgagt tcacaccctc ctgcatacgc gcaccgccgg aagccgccac acagacgaac
     6781 ggacaattgt cggcaaccgc ccggcggacg ccctgtacga atcgttcgcc cacaaccgaa
     6841 cccatcgaac cgccgataaa gcggaactca aacgcagcaa ccacaacggg cagaccgttc
     6901 atcttgcctt tcattaccac caaagcatca tcttccccgg tcagcttgcg tgccgcactc
     6961 aaacgatccg gatatttttt gccgtcttta aacttcaaag gatctgtcgg tttgacattt
     7021 ccggcaacct cctctctgcc atcctcatcc aaaagcagat tcaggcgttg tcgtgccgac
     7081 aacggattgt ggtggttgca tttcggacaa acctgattgt tctgctgcaa ctcggtcgaa
     7141 taaacggttg ccgaacaaga cggacatttg tgccacaaac cctcgggaac attggaagaa
     7201 ccgtctttgc tgcggttttt gattttgggt ggcaggattt tatctaacca gctcatggac
     7261 aactcctaaa atatttagaa tgcgtgacag aatcagcgca cggcatcctt caactctttg
     7321 accaaagcac cgacgaccgc agcctcgttg cccgcattgt tttcaatctc tttcacaatc
     7381 cggctgccga caatgaccgc gtcggcaacc cggccgattt tgcgcacgct ttccgcattg
     7441 ctgatgccga aaccgacacc gatgggaata tcgatatact gatgcaaata ctctatttta
     7501 cgcgaaacct catccgtatc caaacttgcc gcgcccgtta cgcccttgag cgaaacataa
     7561 tagacaaatc cgcctgccag ctcggcaatg gttttaatac ggtcttccgt cgtcgtcggc
     7621 gcaatcagga aaatacagtc gaccccgtta tccttcagct cgcgatagag cggatcgatg
     7681 gtttcgacag gggaatccac cgtcaacacg ccgtccacac ccgcctttgc ggcttcctga
     7741 gcaaactccc gataacccat cttatgtaca gggttcaaat atcccatcaa aacaaccggc
     7801 gtttgcgtgt cggtttcacg gaattttctg acgacatcca agacatcgcg cagcgaaatc
     7861 ccgtttgcca acgcccgctc cgccgcacgc tgaataaccg gcccatccgc catcggatcg
     7921 gaaaacggca cgcccaactc caaaatatcc gcaccgtttg caaccatgcc gtgcatcaat
     7981 gccaaagttg tccgaatatc ggggtcgccc accgtaatat agggaatcaa tgcctttccg
     8041 ccatccaaag cggcaaaagc ctgccggatt ctgctcattt gtctcttcca tcctatcggc
     8101 aaatatgcca taaaacacaa tcagtaaatt tttcccagta taacatttgc ggtacggata
     8161 ggcgaatacg gcaaacagaa accacgcaaa tcagagccgc cgtttgaacg aggcggaaat
     8221 aaacggcaag catctcggat tatagtggat taacaaaaat caggacaagg cgacgaagcc
     8281 gcagacagta caaatagtac ggagccgatt cacttggtgc tccagcacct tagagaatcg
     8341 ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctataacaaa
     8401 attcagaatc aagacagggc aataccatac tggttttgca ccaacccgct ataaaattgc
     8461 ataaaaaaaa tagacgctgt aaaacacagc gtctattttt tatctggcgg agtaagaggg
     8521 attcgaaccc tcgatgcagg ttgaccccac atgcttcctt agcagggaag tgccttcagc
     8581 ctctcagcca ttactcctaa ggaagctgta actataatca acttttccga taccgtcaag
     8641 gaaaaatgga aagcaaatat taatcttttg aaataaaata agatttaccc tgattatacc
     8701 caatcaggca cttcttttct tcctgaatgg aaatattcct gactgccaca tcctcaagca
     8761 aaatgctttt gtgcatcctg tttgatttcc tgaaaccacg cgtcttcagg aaaacgtgca
     8821 agcgcgacat ccaatgttga cagcaggttt tgcgacaggg ctttggtcag gtattcctcc
     8881 tcgttggctt ctgccgcttt gtcgggctgt tgtgccaaac cgtcggcacg ggctttgtgt
     8941 ccgtgtgcca aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     9001 tacagatagt acgacaaggc gaggcaacgc cgtactggtt taaatttaat ccactataat
     9061 tgagcgcggt aattttttgc gcccaaagct gcggtatgtc gggattgtct tcaatcatga
     9121 cggaaaggat ggatacggcg gtcgccagcc tgccgccgtt cattctaagg tcgagggaac
     9181 ggttgggcgg cagggtgctg agtgtttctg ccattctgaa ccaaaattcg ccactggctt
     9241 cgtcgggtgc gttcaattta agcatttcat agtgaacctc ctgatcatcg ttttgcacga
     9301 gaccccatac ttcggcaagg attttctgtt cttcccggct caagtgttgc cacttttcaa
     9361 tatgttgctg catatctttt ccctttgaac ctgttttata cggtctgaaa tacagcgcga
     9421 accctatcca aatcttcctg cgtatccaca ccggcggcgg gggcttcttt ggcggtttcg
     9481 acggcaatcg ggtagccgtg ccacaggacg cgcagctgtt ccagcgattc gatggtttcc
     9541 agcggcgaaa cgctcatttc ggcatagcgt tgcaggaagc cggcgcggta agcgtagatg
     9601 ccgatatggc gcaggacggc ggtttcagac ggcatttcgc gttttcctgc acgcatcgca
     9661 tcgcgcggat agggaatcgg ggcgcggctg aagtagatgg cgttgcggtt tttgtcgagg
     9721 acgactttga cggcattggg attcatcaat tcgtcgaaat cgtgcaattc gtgtgccgca
     9781 gttgccatct gtacgttgtt ttccaccaag acttcggcag tgcggtcgat gagttcgggg
     9841 gcaatcagcg gctcgtcgcc ctgtacgttg acaaccacca gatgcggcgg cagcttcagc
     9901 gcggcagagg cttcggcaag gcgcgtcgtg ccgctttcgt gccggtttga agtcatgacg
     9961 acttcgacgc cgtgcgcctg acaggccgtc tgaatatcgg gatggtcggt ggcaacgacg
    10021 acgcgcacgg ctttactttt tgccgcctgt tcggcaacgc gcacgaccat cggtttaccg
    10081 tggatatccg ccaaggcttt tccgggcagg cgcgatgaat ccagccgcgc cggaatcaat
    10141 acgacgaatt cagtcatgct ttgagttcct cttcgctcaa cgctcgcgct tcgttttcca
    10201 gcatataggg aatgccgtct ttaatcggat aggcaagctt cgcctgacgg ctccacaatt
    10261 cctgtttgtc ctgatgatat tccagcctgc ctttggtaac ggggcagacg aggatgtcta
    10321 agaatttttt ttccataggg ttgctttcgg gtttgatggt tcaatcaatc ggatgccctc
    10381 ttcaatattt cagacggcta tcatttattt ctgatgcctt ttcccaacct gtccggcttg
    10441 tttacagccc gcatcgggat ttttaccgcc gcagatgatg cgccgcaact cttccgtaaa
    10501 cgcgggcaga tgatgtcgcg cgattttccc tgacaatgca gtccaagata ctgctatttc
    10561 actcatcgat ttcttgatta agcgcggatt tttggcaacg acggactgac cgacaggcga
    10621 accgtaaaag gcaatcatgc cgtcaatttc ttcttgcgtg tatatctcac gggcattctt
    10681 caataaagta ttgcggacag cctgtttcac ttcgggcgta atcaaatctt tcaaaacatt
    10741 ctcacgatag cggttaaagg cttttgccgc ctgatctttt ttcgcttccg gcatttcggc
    10801 aagggctttg tccgcatacg gtttaaatcc ggcattaaag ccctcaatca tatttttttc
    10861 tatatcccgg tcaaaattct gcgtatccag ccaacgcgcc aacgacgcgt cgctgggcgg
    10921 agcggcaaaa gcattggtgc acaatgccag cgtggcgaag ggcaataaca aggttttcag
    10981 tttaatattc aatcctttaa tgaatgatta atgcagataa aaaatacgac cggttttctc
    11041 attcctgtcc aagtggaaat ccggagattt gggatttctg aagcctccta ggcattcctg
    11101 aaaattcgag acttagattc ccgcctgcgc gggaatgacg gcgcgaggcg ttcttattca
    11161 aatccgtaat cactccgccc aaaccgtgct ttcagacggc cttcggtaca tcttccaacc
    11221 gctccaacac aaacgccgcc aaatcaggtt cgattatcgc acaaacgggc aacacccaaa
    11281 cattatcggt gcaaatgccg tctgaaaatt tgaccgcatc tttctccgta ataatgaccg
    11341 cgtccgcatc gggcaaatct gccgccgaaa tgtcggcgtg gtcgggcagc gcgacggttc
    11401 gcttcacggt aatgcccata ttccgcaacg aatcaaaaaa ccgcgccggc ttggcaatac
    11461 ctgccacggc gacgacggtt tgatttttca gacggcctgt gtccagtatt tcggacggat
    11521 tgttcaaacg gtaaatccgt cccgcttcga tacggctgtg aaacatattt tcagacggcc
    11581 taaacagcgc atccgccttg ccgccgctga cgacgaccgc atccaccgaa tccagccgca
    11641 acaaaggttc gcgcaaactg ccgttgggca gtaaatccaa atccgtgcgc cccgtatccg
    11701 ccgccggaaa caccgcgatt tccacatctc gccgcagggc gtaatgctgc aaaccgtcgt
    11761 ccgccacaat cagtccgatg tcgggatgcg ccgccagcaa cgccctgcct gcctctgcac
    11821 ggctgctgcc caccgccgtc ggcgcaacgg ttttgcggaa cagcagcaaa ggctcgtcgc
    11881 ccgcatcttc cgcccggctc ttctcattca atacatgaac cgccttgctc ttgcgcccgt
    11941 agccccggct gatgatgccg accttgacac ctttttcctg caaacccgac accagcgcgg
    12001 caacaatcgg cgtttttcct gtaccgccgg catggatgtt gccgaccacg accacaggca
    12061 cgggcagctt ttcgctttgc cgttttcccg ataaaaaatc cgtccgccgt tttgccgcaa
    12121 ttttggcaaa cagcccggag agcggcttaa gcagaaaaga caaaaccgga taaggttttt
    12181 gccaatgccg ttcgataacg gtatgaaatt tgaaacaatt cggcatattc gatacaaatc
    12241 ctcgaagcaa aatcagcccg ggtcattaat catgcgtacc cgatacagac ccacctataa
    12301 acaacgcgaa gaccaaaccg ataagcgaac caatcaggta aataagcaac tgcgctttac
    12361 agaaattggc gcggttcggg ttgcctctgc cgaacaccca aacaaaaatt gaccaaagga
    12421 atcatgaaaa caatcatcgt caacacccac tgccctgtcg tcatgacggg aggcggtttg
    12481 ttttgaagtc tcgggcatat cgccaatatg ataacggaca ggtttcatct ctttcatttt
    12541 ccagtatctt tcatttatag tggattaaca aaaaccagta cggcgttggc tcgccttagc
    12601 tcaaagagaa cgattctcta aggtgctgaa acaccaagtg aatcggttct gtactatttg
    12661 tactgtctgc ggcttcgtcg ctttgtcctg atttttgtta atccactata tcaatctgac
    12721 agcaaaatgc cgcctgaaaa cagttcagac ggcattttaa accgcaaacg gcttacagct
    12781 tccattcgct cagcttggca gcgtaagctt ccaaatctgc aatcggacgg gttgccacgc
    12841 cgctttccat cgctgctttg gcggcagccg tagcaacgcg cggcagcagg cgggaatcga
    12901 acggagtcgg aatcaggtat tccgcgccga attcaaattt cttaccgtaa gcggcaacca
    12961 cttcttcggt tacctcttcc atcgccaaat ccgccaaagc atacacgcag gcgcgtttca
    13021 tttcttcgtt gatggtcgtc gcgccgacat ccaacgcacc gcggaagatg aacgggaagc
    13081 acaatacatt gttcacttgg ttcgggaagt cggagcggcc ggtaccgata accacgtccg
    13141 gacgggtttc tttcgccagc ggcggcagga tttccggatt cgggttggcc atagcgaaca
    13201 cgatgggttt ttcgttcatg gtgttcagta tttcaggcgt cagcaggttc gcgccggaga
    13261 ggcccaagaa gatgtcttta cctttgacgg catcggcaag tacgcgctgg ccattgtctt
    13321 caacggcgta gaattttttg gattcgtcca tgcggtcttt gtcttcgcgg gtttggtaaa
    13381 tcacgccttt ggagtcgcaa acggtcacgt tttcgcgttt caagcccaaa tccagcaatt
    13441 ggttcaagca ggcaatcgcg gccgcacctg cgccggaaca caccaaagtc gcttcttcga
    13501 ttttacggcc ggtaaaacgc agggcgttca atacggcggc ggcggtaatg atggccgtgc
    13561 cgtgctggtc gtcgtggaat acggggattt tgcagcgttt gcgtaattcg cgttcgatgt
    13621 agaaacactc gggtgctttg atgtcttcga ggttgatgcc gccgaaggtc ggctctaaag
    13681 cggcgatgat gtccacgagt ttttgcgggt ctttttcatc gatttcgatg tcgaacacgt
    13741 ccacaccggc gaattttttg aacaatacgc ctttgccttc catcacgggt ttgcccgcca
    13801 gcgcgccgat gtcgcccaag cccaaaacgg ccgtaccgtt ggaaatgaca gcgaccaagt
    13861 tgcctttggc ggtgtatttg taggcatttt gcggatcggc atggatttcc atacaaggag
    13921 cggctacgcc cggagagtac gccaacgcca aatctttgtc ggtcgccaga gatttggtcg
    13981 gggtaacgga aattttgccc ggaacgggta attcgtggaa cttgagggcg gcttctttca
    14041 atgagttttc catgtgtcaa tcctgttgcg agaaaaagat ttgaatcgga ctgtccgaaa
    14101 cgctttcggg tcatcatgtt acgggcggtt cgttaatatt gccgacgcat cctgccttgt
    14161 aaccgagcgt aattctatca tgccgaaagt ttggcgcata ccgccatttt cacctgcggc
    14221 actgccgttt gattttcggc gcgggcggat gcgcttgttt caatatttgc cgcatcaaca
    14281 gccgaaatgt ctgtccaaca ctttttctat gctgtttctg acttcatcca agccgaggtt
    14341 actgtcgata acggcgtaac gttccggaca ggcggcggca cggttcagat aaacgctgcg
    14401 cacacgcatg aagaaatctg cctgttcctg ctcgaaccgg tctttctcgc gcgtctgccc
    14461 gatacgcgcc atcgacactt ccagcggcac atccaacagc agggtcaaat cggggcgcaa
    14521 accgccctgc acccaatgtt ccaaaatttc aatgtcttca gacggcattc cccgcccgcc
    14581 gccctgatag gcgaaggtcg catcggtaaa acggtcggac acgacatgga tgccgtctga
    14641 aagtgcaggc aatatcacgt cttcaatatg ctgcattctg gcggcgaaca tcatcagtgt
    14701 ttccgcacgc aaaccggctt tggtttcagg gttgagcagg atttcgcgca aggcctcacc
    14761 gaccggcgtt ccgcccggtt cgcgcgtgaa cagcacgggc agccccctcc gttcaaacca
    14821 tgccttgatg acggcaaggt tggtggattt gccggcaccg tctatgccgt ccaaagtgat
    14881 gaattgcggt ttcatgcttg cagttccggt aagggtatgc cgtccgaaaa cacaaacttt
    14941 tcagacggca tggtttattt tttcaaaata tatttgcgaa cggcggcgtt gtgttcggtc
    15001 aaatcatggc tgaactggct caagcccgta ccgtccattt tggacacgaa atacaggtat
    15061 ttttcaccgg acggatgggc ggcggcatcg agtgccgcct tgccgggcag cgcgatcggg
    15121 gttggcggca gaccgccgcg cgtgtaggtg ttgtacggcg tgtcgcggcg caggtcggct
    15181 ttacggattt tgcccttgta tgccgcaccc atgccgtaaa tcacggacgg gtcggtttgc
    15241 aggcgcatac cgattttcag gcggttgacg aagacggaag cgacatggtc gcggtcggct
    15301 tcatgccctg tttccttttc gatcaggctc gccataatca gcatttcata aggattttta
    15361 taaggcagcc cgtcctgcct gctttcccat gcctcattca ggcggcgttg catcgccttg
    15421 taggcggttt ggtaaatccg taaatcgctg ccgcccgcat cgatttcgta gctgtcgggg
    15481 aaaaactgcc cttcaggatt gccgctgaag gcatcagggg caacttccgc catcagtttt
    15541 tcattgctcc agcctttggt gtcgtgttcg atgtcgggcg ttgcgtcgat gactttcctc
    15601 atatgcgaaa aacgcgaacc ttcgataatc tgcacggtaa cggaatccgg cctgccgccg
    15661 cgcattttct gcaagatatc ccaagcagac acttccgaag gcagtctgta cgtccccgta
    15721 tgcagcctgt tgtgcacacc caaaacgtag gccgccgccg tcaaaacatg cctgctgaac
    15781 acgatgcggt cttcggcaag tttcctgccg accgacgaaa taccctgatt tttggcaatc
    15841 ttgattcggt atgccctgcc attatcctta ggaacaaaaa gcagcgcggc gaaaacggct
    15901 gccgacacgg tcaaaaaaac ggcagaccat ttcaacaatt ttctcaacat ggtaggattc
    15961 ccagcattcg gaatacgtct taaaattcgg gcgacagtat aacccaaatt gcccgccgga
    16021 caggaagaac atcatggaca atcacgccga agcacactgg caaaacggct ggcttcaaag
    16081 catacgccat accccgtcgc ccaatttcag cccgagggaa acgggggaaa cggtttccct
    16141 gatcgtgttg cacaacattt cactgccgcc gttcgaatac ggcacggatg ctgtggaaaa
    16201 gctgtttgcc aaccggctcg accccaacgg acatccgttc ttcagcctga tacacacttt
    16261 gcgcgtatcc agccatttct taatcaaacg cgacggagaa acggtacagt tcgtttcctg
    16321 cgacgacatg gcgtaccacg ccggcgtgtc ttcattcggc ggcagggaaa aatgcaacgc
    16381 attttccatc ggcatcgaac tggaaggctg cgatttcgaa ccctttaccg aagcgcaata
    16441 ccgttcgctc gaagccttgt tggacgcaat ctgccgccgc taccccatca cggcggtaac
    16501 ggggcaccaa gacatcgcgc ccggccgcaa aaccgacccc ggtcattttt tcgactggcg
    16561 gcggatacgg gggaaagggt ttcccgtgga cagaaatgcc gtctgaaaac cacagatttt
    16621 agacggcatt tcggaaaatt tccgcttatt tcgacacact gctgtcagac cctgcaattt
    16681 tcgttataat tcaaacgtta taaccggccg ttccgtcaga ctgccggaaa cgcaagcccg
    16741 atttaaccgc agtacccgca ctttgcgcat cctgcatccc gaaatttaaa gtgattgcca
    16801 tgatttacat cgtactgttc ctcgccgccg tcctcgccgt tgtcgcctac aatatgtatc
    16861 aggaaaacca ataccgcaaa aaagtgcgcg accagttcgg gcactccgac aaagatgccc
    16921 tgctcaacag caaaaccagc catgtccgcg acggcaaacc gtccggcggg ccagtcatga
    16981 tgccgaaacc ccaaccggcg gtcaaaaaaa cggcaaaacc ccaagacccc gccatgcgca
    17041 acctgcaaga acaggatgcc gtctacatcg ccaagcagaa acaggcaaaa gcctccccgt
    17101 tcaaaaccga aatcgaaacc gccttggaag aaagcggcat tatcggcaac tccgcccaca
    17161 ccgtttccga accccaaacc ggacattccg caccgaaacc tgccgacgcg ccggcaaaac
    17221 ccgttcccgt tccgcaaacg ccggcaaaac cgctgattac gctcaaagag ctgtcgaagg
    17281 tcgagctgcc ctggtttgac gtgcgcttcg acttcatctc ttatatcgcg ctgaccgaag
    17341 ccaaagaact tcacgcactg ccgcgccttt ccaaccgctg ccgctaccag attgtcggct
    17401 gcaccatgga cgaccatttc cagattgccg aacccatccc gggcatccgc tatcaggcat
    17461 ttatcgtggg tattcaggca gtcagccgca acggacttgc ctcgcaggaa gaactctccg
    17521 cattcaaccg ccaggtggac gcattcgcac aaagcatggg cggtcagacg ctgcacaccg
    17581 accttgccgc ctttatcgaa gtggcttccg cactggacgc attctgcgcg cgcgtcgacc
    17641 agaccatcgc catccatttg gtttccccga ccagcatcag cggcgtagaa ctgcgttccg
    17701 ccgtaacggg cgtgggtttc gttttggaag acgacggcgc gttccactat accgacacgt
    17761 cgggctcgac catgttctcc atctgctcgc tcaacaacga gccgtttacc aatgcccttt
    17821 tggacaacca gtcctacaaa ggcttcagta tgctgctcga catcccgcac tctccggcag
    17881 gcgaaaaaac cttcgacgat ttgtttatgg atttggcggt acgcctgtcc ggccagttga
    17941 acctgaatct ggtcaacgac aaaatggaag aagtttcgac ccaatggctc aaagacgtgc
    18001 gcacttatgt attggctcgt cagtccgaga tgctcaaagt cggtatcgaa ccgggcggca
    18061 aaaccgcatt gcgcctgttc tcctaaaatc gaatgcaatc aaagcggata tgtcgttacg
    18121 gcatatccgc tttttttcgg gtaaaattca gctgttttca acctatcatt tttcagacga
    18181 cctttgcccg tttcatcccg tatcagacaa aggtcgtctg aaaaccttct ctcatgaatc
    18241 caaactcaaa acataacact aactttacca atcttcttaa accgtctgat agtgatataa
    18301 aacaatttgc cgcacaacac atccgccacc tcaccgacct gctcaaccgc tatgcctacg
    18361 aatactacac cctcgacgcg cccagcgtac ccgatgccga atacgacaaa ttattccgcg
    18421 agctcgaagc gttggaatta aaccatcccg agctcaaact gcccgacagt ccgacccagc
    18481 gcgtcggcgg cgagccttta gcgggatttg ccgaagtgcg ccacgaagtg ccgatgctgt
    18541 cgctgaccaa cgctttttcc ccgcaagatg aaaacggcgt gttcgaccat gccgaaatgt
    18601 acgctttcga ccaacgcgtg cgcgacggtt tggacggcgg caaacccgaa tacgttatcg
    18661 aacccaaatt cgacggcctc gccatcagcc tgctctaccg cgacggcgta ttggtacagg
    18721 cggcaacgcg cggcgacggc acaacgggcg aagacgttac ccaaaacatc aaaaccgtcg
    18781 ccaacatccc cctgcggctg cacggcgaaa atacgcccga actcatcgaa gtacgcggcg
    18841 aagtactgat gcaaaaagcc gattttgccg ccctcaataa aagacaagcc gaaaacgggc
    18901 aaaaaccctt tgccaatccg cgcaacgccg ctgccggcag cctgcgccaa ctcgattcgc
    18961 gcatcactgc gcaacgcaaa ctgcactttt tcccctactc cgtcgcccgc cagcaaggcg
    19021 gtttcgtcgc ggaagaacac atccaagaac tcgcctattt ccaagcactc ggcttcagtc
    19081 tgccaaatgg caatttcggc tgtttcaaaa atatcgacga agtattggcg ttttacgaac
    19141 aaatgcagca aaaacgcccc gaactgccct acgaaatcga cggtatggtg gtcaaagtca
    19201 acagcttggc gcaacagcgc gaactcggct tcatctcacg cgcgccacgc tgggcgattg
    19261 cccacaaatt ccctgccgaa gaagccctga ccattgtcga agcgattgac gtgcaaatcg
    19321 gcaggacggg cgcggtaacg ccggttgccc gattacaacc cgtattcgtc ggcggcgtaa
    19381 ccgttaccaa cgccaccctg cacaatcagg acgaagtatc gcgtaaagac gtgcgcgtcg
    19441 gcgataccgt cgtcgtgcgc cgcgccggag acgtgattcc cgaagttgtg cgcgtgattt
    19501 ttgaacgccg cccgatgcgg gaaaccgccg ttgccgtttc agacggcatc gggcatcggc
    19561 aggacgattt gtttgccgaa acaccgtccg ctaatcaaac ccaatccgtt ccgctccaca
    19621 agccctaccg cctgccgacc cattgcccca tctgccgcag cgaaatcgaa cgcgaagaag
    19681 gcgaagccgt cgcccgatgc agcggcggca tgctttgtca ggcacaacgc gcgcaaggct
    19741 taatccactt cgcctcgcgc aaagcgatgg acatcgacgg cttgggtgaa aaacaaatcg
    19801 aacagcttgt cgcccaagac ctcgtccgcc acttcgccga cctctaccgc ctcgatattc
    19861 cgaccctgca aaaaatgaag gaagcggcgg acaaaaccgt tgccgaatcg gatcaaatgc
    19921 cgtctgaagg cagttcggtc ggtgcgtcgg gcaaacataa aaaacaaccg accaagtggg
    19981 cggaaaacat cctcgcaggc atagaatccg gcaaaacgcc cgaactcgcg cgcttcctgt
    20041 tcgcgctcgg catccgccac gtcggcgaac gcaccgccaa aacgctggca caggcattcg
    20101 gcacattgga acgcgtccgc cgcgcccccg aacccgtcct cgcctgcctg cccgacatcg
    20161 gtacggtagt ggcgcgttcc attgcccact tcttcgccca agccgaacag caggcgatga
    20221 tagacgagct gctcgccgca ggcgttgccc cgcaagccca agccgtcagc ctgcctgccg
    20281 cacaatatgc cgaaccgcaa cgctggattg cccgcctgcc cggtttcaaa attagcgaaa
    20341 acaaagccca agccttatgg gaactcgccg gcaaaagtat agagggtctg caaaccgaca
    20401 aatccctccc cgccgactgg caagcatggc gcagcgaacc acaaaacgcc gccctactcg
    20461 aaaacctgaa aaccttcttc gcgcaaatgc cgtctgaaga cgaggcggcg caaggttcag
    20521 acggcatcaa taaagccgta gcgggcaaaa ccttcgtgtt aactggcaca ctgcccaccc
    20581 tcaaacgcga ccaagcccaa tccctaatcg aagccgcagg cggcaaagtt tccggcagcg
    20641 tttccaaaaa aaccgattac gtcgtcgctg gagaagccgc gggcagcaag ctggaaaaag
    20701 ccaatgcctt gggtgtttcc atcctcagcg aagccgaact gcttaccctg ctcggctgaa
    20761 cctgtgccgt ctgaaccgcc ttcagacggc attgtccact tttccgaacc acaccatgca
    20821 caccatttcc tttccaaacc gcacccgact caccgccctg ccgcccctgt cgctttacat
    20881 ccacatcccg tggtgcatca aaaaatgccc gtattgcgac ttcaattccc acagcctgaa
    20941 aaacggattg cccgaagccg cctatatcga cgcgctgctg accgacttgc agcttgaatt
    21001 gcccaatatt tggggcagac cggtggaaac gatctttttc ggcggcggta cgcccagcct
    21061 gtttcaggcg gaatcgattg accgtttgtt gagcggcgtg cgttcgctgt tgcgcttgca
    21121 gccggaagcg gaaattacgt tggaagccaa tccggggacg tttgaaatcg agaagtttca
    21181 aggatttaaa gacgcaggca tcacgcggct ttctattggc gtacagagtt tcaacgacga
    21241 tatgctctca aggctggggc gcgtccacaa cggtaaggaa gccttaaccg ctatcgatac
    21301 cgccttgaaa ttatttgata aagtcaatat cgacttgatg tatgccctgc cgaaccaaac
    21361 cgttcagacg gcattggacg acgtacaaac cgccatcgct acgggtgcga cccacatcag
    21421 cgcgtatcat ctgacgatgg aaccgaacac gccgttcggt catacgccgc cgaaaggttt
    21481 gccgcaagac gaagccgccc tcgacatcga agacgcggta cacggcgcgc tggaaagcgc
    21541 gggttttgtc cactacgaaa catcggcttt tgcgaaacca gccatgcagt gccgccacaa
    21601 tttgaactac tggcagttcg gcgattattt aggcataggc gcgggcgcgc acggcaaaat
    21661 ttcctatccc gaccgcatcg agcgcaccgt ccgccgccgc caccccaacg actacctcgc
    21721 cttaatgcaa aaccgaccga gcgaagccgt cgaacgcaaa accgtcgccg ccgaagattt
    21781 gccgttcgaa ttcatgatga acgccctgcg cctgaccgac ggcgtaccca ccgcgatgtt
    21841 gcaggagcgc acgggcgtac cgagtgccaa aatcatggcg caaatcgaaa cggcaaggca
    21901 aaaaggcctg ctggaaaccg accccgccgt attccgcccg accgaaaaag gacgcttgtt
    21961 tttaaacgat ttgctgcagt gttttttata gtggattaac aaaaaccagt acggcgttgc
    22021 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
    22081 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
    22141 ataagcgcaa acaaatcggc ggccgcccgg gaaaaccccc ccgaacgcgt ccggaaaata
    22201 tgcttatcga tggaaaacgc agccgcatcc cccgccgggc gtttcagacg gcacagccgc
    22261 cgccggaaat gtccgacgct taaggcacag acgcacacaa aaaaccgtat gcctgcacct
    22321 gcaacaatcc gacagatacc gctgtttttt ccaaaccgtt tgcaagtttc acccatccgc
    22381 cgcgtgatgc cgccaccacc atttaaaggc aacgcgcggg ttaacggctt tgccgtcggc
    22441 aaagcagccg gataccgcta cgtatcttga agtattaaaa atattacgat gcaaaaagaa
    22501 aatttaagta taataaagca gaattcttta acggattctt aacaattttt ctaactgacc
    22561 ataaaggaac caaaatatga aaaaagcact tgccacactg attgccctcg ctctcccggc
    22621 cgccgcactg gcggaaggcg catccggctt ttacgtccaa gccgatgccg cacacgcaaa
    22681 agcctcaagc tctttaggtt ctgccaaagg cttcagcccg cgcatctccg caggctaccg
    22741 catcaacgac ctccgcttcg ccgtcgatta cacgcgctac aaaaactata aagccccatc
    22801 caccgatttc aaactttaca gcatcggcgc gtccgccatt tacgacttcg acacccaatc
    22861 gcccgtcaaa ccgtatctcg gcgcgcgctt gagcctcaac cgcgcctccg tcgacttggg
    22921 cggcagcgac agcttcagcc aaacctccac cggcctcggc gtattggcgg gcgtaagcta
    22981 tgccgttacc ccgaatgtcg atttggatgc cggctaccgc tacaactaca tcggcaaagt
    23041 caacactgtc aaaaacgtcc gttccggcga actgtccgcc ggtgtgcgcg tcaaattctg
    23101 atatgcgcct tattctgcaa accgccgagc cttcggcggt tttgttttct gccaccgcaa
    23161 ctacacaagc cggcggtttt gtacgataat cccgaatgct gcggcttctg ccgccctatt
    23221 ttttgaggaa tccgaaatgt ccaaaaccat catccacacc gacaaagccc ctgccgccat
    23281 cggcgcatac agccaagccg tccgagcagg cgacaccgtt tacatgagcg gtcaaatccc
    23341 cctcgatccc gccacgatga ccgttgtcgg caacggcgat ttccgcgccg aagcgcgcca
    23401 ggtctttaaa aacctgcagg ccgtcgccga agctgcaggc ggcacgctgg ccgacatcgt
    23461 caagctcaac gcctacctga ccgaccttgc caacttcgcc gtcttcaacg aagtgatggc
    23521 ggaatttatt gccgagccct tccccgcccg cgccgccgtc ggcgttgcct cgctgcccaa
    23581 aggcgtgcag gtcgaagccg aagccgtcct cgttttgaac gcataattcc acgttcagac
    23641 ggcatgatat ttcaagaccg tgccgtccga accgcaacac tttcccactt cacaacatct
    23701 gcaatggcac attacgcaat cggcgacatc caaggctgtt tcgacgaact gaccgcgctg
    23761 ctcggcaaaa tcggcttcaa ccacggcaca gacaccctct ggctgacggg cgacatcgtc
    23821 aaccgcggcc cgaaatccct cgaaacgctg caattctgca tccggcacga aaacagcgtg
    23881 caaatcgtcc tcggcaacca cgacctgcac ctgcttgccg tcggctacgg cgaaggcgcg
    23941 cccaaacgca gcgacacaat cgaacccata ctcaaacacc ccgacggcag aaaaatgctc
    24001 gactggctgc gcgcgcaacc gctgttgata cgcgagggca accgcgtgat ggtacacgcc
    24061 ggcatcctgc cgcaatggcg cataaccaaa gccgaatcgc tcgccggaga agccgaagcc
    24121 gaactgcgcg gcaaaaaata cgtcaaattc ttctccaaaa tgtacggcaa caaaccggcc
    24181 gcatgggacg aaggtttgga aggctatgcc cgcctgcgct tcatcgtcaa cgccttcacg
    24241 cggatgcgcg ccctgacctt taaaaacgaa ctggatttcg actacaaatc cacagtgaaa
    24301 aaaatgccgc tttacctgcg cccgtggttc aaagcccccg accggcaaaa cctcgaccac
    24361 accatcatct tcggacactg gtcttccttg ggctacacga atgccgacaa cgtcatctcg
    24421 ctggacaccg gcgcactgtg gggcggacag ctgaccgccg tcaatctcga aacggaagaa
    24481 attacccaag tccaagccgc cggcggcata gactggaaaa gcttcacaaa ataacggacc
    24541 gccccactat gcacaaaatc ctgcccatcg cccacgtcct ctcccgactg ggtatgctgt
    24601 tttcctttat cctgctgata cccgccgccc tctcctacgc cttttcggac ggcgcgtaca
    24661 ccgccttcgc caccaccgcg accgttaccc tttccggctc gtacatcgtc cggctcgcca
    24721 ccctccggtt caggcgcgaa ctgcgcccgc gcgacggctt caccctcgtc ctgatgttgt
    24781 ggctggcgtt tgccgctatg gcggcgatgc cgatgtacct gtatttcccg aatatgggct
    24841 ttaccgacgc attttttgaa tcgatgtcgg gactgaccac caccggcgcg accgtcatcc
    24901 cccacgtcga cgggctcgcc ccctccgtca acttttggcg gcatatgctc aactggctgg
    24961 gtgggatggg catcatcgtc cttgccgtcg ccatcctgcc tatgctcggc gtaggcggca
    25021 cgcagttatt caaagccgaa atccccggca ttgacaaaga aagcaaaatg tcgccgcgca
    25081 tttcccaagt ggcgaaaaaa ctctggttcg gctacaccct gatcaccatc ctcgcggcag
    25141 cctgcctgca ttttgccggg atgagctggt tcgatgccgt ctgtcacgca atggcgaccc
    25201 tctcgctggg cggattttcc acccacgatg ccagcatagc ttattacaac tcccccctca
    25261 tcgaggcggt catcatcgtt ttcaccattg tcggcggcat caattttgcc agccatttcg
    25321 ccgccctcaa cagccgcagc ctcaaaacct attggaaaga cgaagaatgc cggacgatgc
    25381 tgctgctgct ctccggcagc atccttaccg ccgccctgta cctgtggcac accggctatt
    25441 acgccggttt taccgaatcc ctgcgctaca ccgccttcaa cttcgtctcc atcggactgg
    25501 caaacgggct ggccaacacc gacttcgcac aatggccgct cctgatttcc ctgtggatgt
    25561 ttttcctcgc caacatcctc gccaactccg gctcgaccgg cggcggcatc aaaaccatac
    25621 gcgccctcgt cctgttcaaa ttcagcctgc gcgaaatgat ggtgctgctg caccccaaag
    25681 ccgtccgcac cgtcaaaatc agcggcaagg ccatacccga ccgcctcgcg ctgaccgtta
    25741 tgtccttcat cttcatctac tttatgaccg tcgtcctctt cagcttcctg ctgatggcga
    25801 gcggtatgga atttaccacc gccttcaccg ccgtcatcgc ctgcatcacc aacgccggcc
    25861 ccggcctggg cgaagtcggg cccgccggca attacgccgg tttggacgtg atgcagaaat
    25921 ggatctgcgt taccgccatg ctcttgggca ggctggaaat cttcaccgtc ttcatcctct
    25981 tcacccccgc ctactggaag aaataaaaaa tgccgtctga aaacttttca gacggcatct
    26041 tgtcctttaa agaagcggaa gctctacaaa aggaaagaaa tgctgaaacc tctgataaat
    26101 ttcaggatat ttctctaagg cttttaatgc tttttctttt gaaataggcg gatcatagac
    26161 atctatcccc cttaagaagg caatgcccgt taatgcattt ttttgatcat ttgaaatgta
    26221 accataatta acgactccaa ttacatcata aatttcccct atactcttat caaaatcaat
    26281 caaatcaaaa atatacaatg gaattgtatc aataggagta tctctgacaa ctatatccaa
    26341 ccataattta aattcagaaa gattaatagc atcatagaac atacagacta ttgcaaatcc
    26401 taaatcttca caattttctt tactaatttt ccacataatt atttaccttt atccattgct
    26461 tcccaaccga tacatccggt attttagctt ctccttttcc gtcaaacact cctttggtta
    26521 cctgctttgt gtcaaaaatg ccgtctgaaa gccgaatatc atttcagacg gcattttgac
    26581 tgtttaaagc gggggcagtt ctacaaacgg aaagaaatgc tgaaacttct gataaatttc
    26641 aggatgtttc tttaaggctt ttaatgcttt ttcttttgaa ataggcggat catagacatc
    26701 tatccccctt aagaaggcaa tgcctgtcaa ggcatttttt ttcgatttcg gcaggctgta
    26761 actcgaaaca aaatctacaa tattgtcaat gtctccaatt cccccatcga aatccgccaa
    26821 gtcaaaaata taaaaaggga tgtcctcgat gggcatatct cgtattactt gttcaatcca
    26881 taatttgaat tcatttaaat caatagattg agagaataag catttaattg caaattctaa
    26941 atcatcacta tcctctttta tgattttcca cataattatc ttcctttgtt catcattatc
    27001 ttcctttgcc gtcaaacact cttttggtta cctgctttgt atcaaaaaat gccgtctgaa
    27061 agccgaatat tgtttcagac ggcattttga ctgtttaaag cggaggcagt tctacaaatg
    27121 gaaagaaatg ctgaaacttc tgataaattt caggatattt ctctaaggct tttaatgctt
    27181 tttcttttga aacgggagga tcatagacat ctatacccct taagaaggca atgccggtca
    27241 aggcattttt tttcgatttc ggtaggctgg aactcggagc aaaacctaca atattgccaa
    27301 tatcgccaat ttccacatcg aaatccgcca aatcaaaaat ataaaaaggg atgtcctcga
    27361 tgggcatatc gcgtattact tgttcaatcc ataacttgaa ttcatttaaa tcaatagatt
    27421 gagagaataa gcatttaatt gcaaatccta aatcatcact atcctctttt atgattttcc
    27481 acataattat cttcctttgc cgtcaaacgc tcttttagtt acccgcttta tatcaaaaat
    27541 accatctgaa agccgaatat cgtttcagac ggcattttga ctgtttaaag cgggggcagt
    27601 tctacaaacg gaaagaaatg ccgaaacttc tgataaattt caggatgttt ttttaaggct
    27661 tttaacgcct tctccctagt aatcggaata tcgtaaaacc cttgattccg tatataggta
    27721 atgccatata cggcatcttt ttcctttttc gtcataccgg tagttggaac aaagccaata
    27781 atattatata tatttatgga acaatcatct gattccaaac aatctattaa atcaatcaga
    27841 taccttggaa aactttcggc agggacagtc ctgacaattc catataacca atccaagaat
    27901 tcttttttgt cggctgcatt gcgctcataa cacaatatag caaatcccaa atcataacaa
    27961 ttgtctcgtg taagccccaa cataatcatt ttcctttatc cattgcttca ccacctatat
    28021 gccctgtttg cgtatgcttt ctttcggata ttaactgcat tcgtcccgta tcctgatgat
    28081 gatgccaagt atagctcggt attttagctt ctccttttcc gtcaaacact cctttggtta
    28141 cctgctttgt gtcaaaaata ccgtctgaaa gccgaatatt gtttcagacg gcattttgat
    28201 tgttgggttt ttaatcagag ctttgtttga taatttgtta ttttgaataa aatacaggtt
    28261 ctgtcgagct gataacttct attacagaat cgctaaaaaa gaaaatataa gccttataat
    28321 atttcttttc aaaaaaatca aaactttctt ctttcaacca gtccatataa ctgtcatcta
    28381 ttatctcgaa aaaataattt gcagtaggat tcccatctga attattttct tctttatttt
    28441 caaataaata ttctgaaaat tttactttat aagattcatc tgttatttga aaatgaatta
    28501 tattgtcaat aaatatttca atatttaatt cataatttcg gtcggatgcc aaaatgttca
    28561 catcttgttc ggtaacacaa attttgtcta cgtaaaatct gtgattattg ttagaaaatt
    28621 gtaatggttt aatcttcact atcgttttcc atatctgatt tttgttttct tattgccctg
    28681 ccggataaag caggtctgcc tccgtactgt ctatcctcac atttactttc ctgccatcgg
    28741 cttttatctc cccggtttta ccgtttttgc caacttcgcg gctttggcgg cggcaatgtt
    28801 gaaggcggct tcgacggttt cggcggcatt ggggttgacc cgtactcatc cgcaaacggc
    28861 aacacgacgg acggtgtttg cttttcagcc gtctgccgtt ttgaccaagg tgctgccgtg
    28921 gaaggagcaa gtgtaggatt tttgaagaac tcagttctca atatcctatt tcaaacgaag
    28981 gaaatgccgt ctgaaaatta aacacggttt cagacggcat tttgattgtt gggttttcaa
    29041 cccgacctac ggttcctaat gattttcaga taaaaataaa gaaatatctt ctaacgcatc
    29101 ttcagcccta ccttgaagct ctggatcatc ttgctttgaa tgtagtaaag aaataacttt
    29161 atcgtaatca ccgatatttg aatgtagcct ggcgagatgt ccgaaacagg ttagcgataa
    29221 tccggcaata tccaagtctt tatttttcga taattccatc aatttctcag aaatccacgg
    29281 tacttcatat gctttggaat taaataccat atcaagaatg ccatctataa cactttgttt
    29341 atcatcagaa gaaatcaact tttctgtttt acttttatca gtcatttttt aatttttcct
    29401 tttgaattaa ctaatccaat cctgcttttt ttaaagcatt tttctgatcg gtatgcaaat
    29461 cttgccaact tctgacatgc ccgtgatatt ccgcaaaacc gttattaaaa gtttgagttt
    29521 tgtctaatac aacgatttca ttattggctt tatcaacccc aactcttcga ggagaagttg
    29581 atttaacttg aacggaatta tcaagagctg cttgtccgtt tgttggtgct ctacttttca
    29641 cagaattatc atttttgcca tgataagaag cgtctttata tcgttggttg cctttatcat
    29701 acggacaatc attatgaacc caaacccctt ccgtttccgc ctgactgccc ttgacgaagt
    29761 aagtatgcca gtcggcaacg gtcagattgt aggcttgcag cggttccggt ttgacggtaa
    29821 tgctttgaac ggtctgctcc gcaccgtttt cggcaaatag cctgcttccc gctttcaaat
    29881 cttccgcttt aatccatttg ccgtccgaat aaaacgggtg gatgcggttg gaaatcaggg
    29941 tttgactgtt gtcgaggccg tctgaaactt caatgtaaac ggtttcttga tacggattgc
    30001 cgtatcgggc ggtaacaggt ttgtatcccg tttctccgct tgcctcgtcc ttggaaagga
    30061 cgcggtcgcc ggcttgaata tgggcaatgg cttttcagcc gtctgccgtt ttgaccaagg
    30121 tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac tcagttctca atatcctgct
    30181 tcaaatgaag gaaagaccgt ctgaaaaaca agcttaattt cagacgacct ttaatcaaat
    30241 ttaagaaagc ccttaccaag caaacggttt ccgcttcata tccgaaaggt tgtcaacttc
    30301 attattcagc aagaactgct caaaagcatt ccaacctttc ttttccacca attcggcttc
    30361 ctgtttatac aaggggacaa gcaaagggaa aacgatattg tagtgttcgc cataacagac
    30421 ctgaaaatca tcgtcgaaat aaaatggggc ggaaacatac agtgcatcca tttttgttga
    30481 atcaacaatg cttctgggca aataaacaac ctgcccccga agaaggattt ttttgttctc
    30541 aatgatggtt tctgccaacc atcttaaaaa tgggaaaatc tgattttcat catattttaa
    30601 agaacatacg aaaagtattt caaatctgct tttataattc agatcatgtt tgcttaaccc
    30661 taaagtaata taagttttta aatccctgtt aggggaagag ggaatagctg cgatatttaa
    30721 tttgtactga cttcccgaat cgcttttcga gccatgagaa atagtgccta tatatttttc
    30781 aatatgatta atcatatttt tcttagcttc ccatcactgt atcgaataat catagaccag
    30841 attgagacaa aacgccttgt tcccattgat agaaagtccg tctgaaaagt aaaagtgttt
    30901 cagacggact acgtttgccg actatccgac ccactttatt atccaatgag attcttgcag
    30961 gtattctttt aatatttcta agttttcgac ccagtcatcg taactgtcaa atgatgagaa
    31021 tttgtcattt gaagataaca gtatcagaaa tccaccggta ttttcctggt catcaataat
    31081 ttttatgaat ttttcttcag gattgacccc attcaggata acccccaaag tattgaattt
    31141 tatgtccata gacacaccct aatctttaat gggaatatgg ttttttcaac ccaacctaca
    31201 ctttttatta gttcccatac gaaccaaaac cttcataatt acaatcattt ttttgtgcaa
    31261 taccaaataa cgtagtttca atagctaaaa tgttttcaag ggaagggacg atattgacgg
    31321 aacaatagca agtccacatt tctccgaatt catcttgttc aatactgatt tgaaaatctt
    31381 gccgataaga aatgagttgg ctttctattg attgcagatt tttttgagaa cctcctataa
    31441 aaaactctat tatcataggt tttgtcaaat caaaccctag ttcttgatat tgtttcagta
    31501 catctgaagt agatttttcg tcaacaatca tttttaatct ccatcttgtt ttcggtattt
    31561 tttccccggg ctttcaattc gattgtaatt attggtactt ttggaaccac cgtgagcgtc
    31621 aattcttcta gattcatctt tgaatgcctc tcgattattt ttggcaggag taaaatctgt
    31681 agctatatga gggtcattat taaccctagc ttctcttgct ccagagattt gggaacgttg
    31741 tcttgtgccc tttccagaat aagttcgacc actttcatga gtattagtat aagaaccaac
    31801 ttctgtccta tatttttctc catacggaca atcattatga acccaaactc cttccgtttc
    31861 cgcctgattg cccttgacaa agtaagtatg ccaatcggca acggtcagat tgtaggcttt
    31921 gagcggctgc tgtttgaggg taatgttttg aaccgtctgt tttgcaccgc tttcggaaag
    31981 cagggtgtcg ccttttttca gacgacctgc ctgtatccat tttccttgac tgtaaaacgg
    32041 gtggatttta ttggaaatca gggtttggtt gttgccgatg ccgtctgaaa tttcaatgta
    32101 aacggtttct tgatacggat tgccgtatcg ggcggtaacg ggtttgtatc ccgtttttcc
    32161 gcttgcctcg tccttggcga agacgcggtc gccggttcgg atacgggcaa tggctttgta
    32221 gccgtctgcc gttttgacca aggtgctgcc gtggaaggag caggtgtagg atttttgaag
    32281 aactcagttc tcaatatcct atttcaaatg aaggaaatgc cgtctgaaaa ttaaacacgg
    32341 tttcagaaga cattgtgatt gttggttttt taacctacgc ttgcgaaatt aattcctttc
    32401 tcctgaatac tgcaccattt atttggttta actccataga agaatgatta gaataaatac
    32461 attcaaagtc agcttcatca aaatattctc cacacataga ccataactct gtaatcaaat
    32521 attccatatc ttcatggtct tctgtgatgg cttctcttga taaataattt ttgattttta
    32581 aaaaccttct tccttcctcg taatctacgg taattaacct gatatctgag tgtatcatgc
    32641 ccagcattgc tatattcata ccgactcgca gccagcttgg taatatattc ataactattg
    32701 caccttttta atggttttag ggttagccgg aacaatgtga actccatttt tacctgaatg
    32761 aatcgtacca aaattggtac ttaatcctga tttcccatcg ctgcctatag gatacccaaa
    32821 atcaacaaca ggatttcctc ttgctccttt agaaataact ggataagcac ctgaatgtat
    32881 tccgttcaac aattcttgag gattaatgtt ttgatttaaa gtactcttac cttcaatgta
    32941 gtttccatgt cctgaaatat gtttcccttg agctccatca tgaattttag tcccaataga
    33001 ttttgcttta aaatcaacat tgcgggtttg aaacatttct ccatctacag actgagataa
    33061 tcttgaagca gtgttatgct tgtaggagtc tgagaaatcc ccgctaaccg cagccttccc
    33121 cggttttgcc gcctttgtca ggtttttgac tttggctgct gcggcaacgt tgaagacggc
    33181 ttcgacggtt tcggcggcat tggggttttc ctgtatccac cggtcaacgg cttcgcgcgt
    33241 attcttttca aagcccgcca cgctgcccaa gccgccgatg acggcgaatt tgccctcggc
    33301 ggg
//
LOCUS       DAWFIT010000011        32965 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFIT010000011 DAWFIT010000000
VERSION     DAWFIT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.011"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(338..4507)
                     /locus_tag="WGQ85_000505"
     CDS             complement(338..4507)
                     /locus_tag="WGQ85_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0750000.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WGQ85_000506"
     CDS             complement(4562..6409)
                     /locus_tag="WGQ85_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0750001.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WGQ85_000507"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WGQ85_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0750002.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WGQ85_000508"
     CDS             8315..9808
                     /locus_tag="WGQ85_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0750003.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGE
                     VLHFHTENGRSYPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGN
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9884..10099)
                     /locus_tag="WGQ85_000509"
     CDS             complement(9884..10099)
                     /locus_tag="WGQ85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750004.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(10092..10307)
                     /locus_tag="WGQ85_000510"
     CDS             complement(10092..10307)
                     /locus_tag="WGQ85_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0750005.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGQ85_000511"
     CDS             complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WGQ85_000511"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0750006.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            12052..12192
                     /locus_tag="WGQ85_000512"
     CDS             12052..12192
                     /locus_tag="WGQ85_000512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750007.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            12189..12989
                     /locus_tag="WGQ85_000513"
     CDS             12189..12989
                     /locus_tag="WGQ85_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0750008.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            13029..14015
                     /locus_tag="WGQ85_000514"
     CDS             13029..14015
                     /locus_tag="WGQ85_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0750009.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14672..14934
                     /locus_tag="WGQ85_000515"
                     /pseudo
     CDS             14672..14934
                     /locus_tag="WGQ85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14972..15346)
                     /locus_tag="WGQ85_000516"
                     /pseudo
     CDS             complement(14972..15346)
                     /locus_tag="WGQ85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15519..15875)
                     /locus_tag="WGQ85_000517"
     CDS             complement(15519..15875)
                     /locus_tag="WGQ85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0750010.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(16018..16362)
                     /locus_tag="WGQ85_000518"
     CDS             complement(16018..16362)
                     /locus_tag="WGQ85_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750011.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGFFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16521..16921)
                     /locus_tag="WGQ85_000519"
                     /pseudo
     CDS             complement(16521..16921)
                     /locus_tag="WGQ85_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16925..17665)
                     /locus_tag="WGQ85_000520"
                     /pseudo
     CDS             complement(16925..17665)
                     /locus_tag="WGQ85_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17735..18052)
                     /locus_tag="WGQ85_000521"
     CDS             complement(17735..18052)
                     /locus_tag="WGQ85_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750012.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(18049..18660)
                     /locus_tag="WGQ85_000522"
                     /pseudo
     CDS             complement(18049..18660)
                     /locus_tag="WGQ85_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18744..19046)
                     /locus_tag="WGQ85_000523"
     CDS             complement(18744..19046)
                     /locus_tag="WGQ85_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750013.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(19225..19431)
                     /locus_tag="WGQ85_000524"
     CDS             complement(19225..19431)
                     /locus_tag="WGQ85_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750014.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGQ85_000525"
                     /pseudo
     CDS             complement(19397..20146)
                     /gene="mafB"
                     /locus_tag="WGQ85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20572..20916)
                     /locus_tag="WGQ85_000526"
     CDS             complement(20572..20916)
                     /locus_tag="WGQ85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750015.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20954..21298)
                     /locus_tag="WGQ85_000527"
     CDS             complement(20954..21298)
                     /locus_tag="WGQ85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750016.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(21301..21489)
                     /locus_tag="WGQ85_000528"
     CDS             complement(21301..21489)
                     /locus_tag="WGQ85_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0750017.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21559..21894)
                     /locus_tag="WGQ85_000529"
                     /pseudo
     CDS             complement(21559..21894)
                     /locus_tag="WGQ85_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(22000..22371)
                     /locus_tag="WGQ85_000530"
     CDS             complement(22000..22371)
                     /locus_tag="WGQ85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0750018.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(22368..22619)
                     /locus_tag="WGQ85_000531"
     CDS             complement(22368..22619)
                     /locus_tag="WGQ85_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750019.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(22751..22957)
                     /locus_tag="WGQ85_000532"
     CDS             complement(22751..22957)
                     /locus_tag="WGQ85_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750020.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGQ85_000533"
                     /pseudo
     CDS             complement(22967..23812)
                     /gene="mafB"
                     /locus_tag="WGQ85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(23909..24121)
                     /locus_tag="WGQ85_000534"
     CDS             complement(23909..24121)
                     /locus_tag="WGQ85_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750021.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(24197..24556)
                     /locus_tag="WGQ85_000535"
     CDS             complement(24197..24556)
                     /locus_tag="WGQ85_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0750022.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(24561..25277)
                     /locus_tag="WGQ85_000536"
                     /pseudo
     CDS             complement(24561..25277)
                     /locus_tag="WGQ85_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(25494..25877)
                     /locus_tag="WGQ85_000537"
     CDS             complement(25494..25877)
                     /locus_tag="WGQ85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750023.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGQ85_000538"
     CDS             complement(25843..27288)
                     /gene="mafB"
                     /locus_tag="WGQ85_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0750024.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGQ85_000539"
     CDS             complement(27328..28290)
                     /gene="mafA"
                     /locus_tag="WGQ85_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0750025.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(28474..29193)
                     /locus_tag="WGQ85_000540"
     CDS             complement(28474..29193)
                     /locus_tag="WGQ85_000540"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0750026.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            29289..29396
                     /locus_tag="WGQ85_000541"
                     /pseudo
     CDS             29289..29396
                     /locus_tag="WGQ85_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(29518..30372)
                     /locus_tag="WGQ85_000542"
     CDS             complement(29518..30372)
                     /locus_tag="WGQ85_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0750027.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGQ85_000543"
     CDS             complement(30501..31229)
                     /gene="rpsB"
                     /locus_tag="WGQ85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0750028.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(31430..31639)
                     /locus_tag="WGQ85_000544"
     CDS             complement(31430..31639)
                     /locus_tag="WGQ85_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750029.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            31717..32298
                     /locus_tag="WGQ85_000545"
     CDS             31717..32298
                     /locus_tag="WGQ85_000545"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0750030.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            32359..32949
                     /locus_tag="WGQ85_000546"
     CDS             32359..32949
                     /locus_tag="WGQ85_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0750031.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         7952 a         7545 c         8048 g         9420 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccta aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttaatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc accgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ttcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcgacggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggcacgaa ggcggacgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcagccttca
     3901 ggtcgaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttgtcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgaac
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg tggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaactaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atattttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggca aaccggcaaa aggcgaaatg cttgctggca cggcagtgta
     9481 caacggcgaa gtgctgcatt tccatacgga aaacggccgt tcgtacccga ccagaggcag
     9541 gtttgccgca aaagtcgatt ttggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg
     9661 gaattggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggtc cggccggcga
     9721 ggaagtggcg gggaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ttgatgccgt ctgaagcgag ggcgttcaga
     9841 cggcattgtg atgtttgggt cgcgggtccg gttcaagtcc gcatcagttg ataaacccgt
     9901 cgtctttcat tttcttttcg gaaaaatatt gaccgcatac cagtaaggcg gcgaggatga
     9961 tgacaaacag ccccgccata gtcagtgtga ttttgaagaa caattccgct tcgagaaaag
    10021 aaaaccaaag ttgcacaatc agcagcagca cccagccggc aaacagtccg agacaggcat
    10081 aaacgccgag cttaaacacg gtcccgctcc aaaatgacga cgtaggcctc gcgtttgaaa
    10141 aacaacagca tacggcgcaa atctttttcc atcgttacaa cccgccagcc ttcgggggtt
    10201 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    10261 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatagg attcctttta
    10321 acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgcaa aacagcaaac
    10381 cttatttctt attgcccaac tgcggcaata ccgaaccttc gcccaatgcc aacaaaccgc
    10441 tttgcgagta aatgcccagt ttggcgcggg tgtcggtaat gtcgaggttg cgcatagtca
    10501 gttgtccgat acggtcgagc ggtgtgaacg ctgcgtcttc gactttttcc atactcaggc
    10561 gttcgggttg gtaggtcagg ttgggcgatt cggtgttcag aatcgagtag tcgttgccgc
    10621 gacgcaattc gagggttact tcgccggtaa tggctttggc aacccagcgt tgggcggttt
    10681 cgcgcaacat cagggcttgg ctgtcgaacc agcggccttg gtagagcaaa cgtccgaggc
    10741 gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggataccg gtcaccaaac
    10801 gctcgtaggc gatgtggaac aacgccattc ccggggcttc gtagatgccg cgcgatttgg
    10861 cttcgatgat gcggttttcg atttggtcgc tcatacccag accgtggcgg ccgccgatgc
    10921 ggttggcttc gaggaagagt tcgacgggat cagcgtattc tttgccgttc agtgcaaccg
    10981 gcacgccttc ttcaaagcgc acgctgactt cttcgggttt gatttcgacg ttttcgtccc
    11041 agaaggcaac gcccataata ggtttgacga ttttgatgcc cgagttcaaa aattccaaat
    11101 ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggaataggct ttttccaccg
    11161 acattttgta gttgaaaccg ttggcaatca aaaattcgct catttcatga cggccgccga
    11221 gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg ggattggtga
    11281 gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg ccgtcgcccc
    11341 aaatattcac atcgtcttct ttcatggcgg aaacaagcat agtgccggtt acggcgcgac
    11401 ccagaggcgt ggtgttgaag taggcgatgc cgccggtgga aacgtggaac gcgccgcatt
    11461 ggatggcggc gatgccttcg tgtgccagct gtgtgcggca gtcaatcagg cgggcgtttt
    11521 ccgcaccgta ttccattgct tttttgggaa tggcattgta gtcgtcttcg tcgggctggc
    11581 cgaggttggc agtgtaggca taaggtagcg cgcctttgag tttcatccac aacagcgcgg
    11641 cagaggtatc aagaccgccg gagaaggcga tgccgacttt ctgaccgacg gggaggtttt
    11701 gtaaaatagt atggttttgg ctcataaggg tatccttcgg acttggaaaa aggagttcgt
    11761 cgtgtttcct tttcgattga aaccccgcca cttggacatc tgtccttcgg ggcggtagaa
    11821 tcagacttta tttgggaggg gcgtaatccc ttccaaatca ggacggcaca tagggcggtg
    11881 ctttatgtgt cgtcccgtgt gttgaaacat tatcttataa atataaccca ttgtcagccg
    11941 ttaaggctgt ctgaaaagaa gctttgcccg tttcgctgaa aatcttttca gacggtcttt
    12001 acgccatcct caacctgact ccctcccctg cgggggaggg ttggggagag ggtgcagaca
    12061 gcagccctta agcaaacgct gcctgaaaaa cgcaagctcg accctctccc taaccctctc
    12121 ccatgggaga gggaacaaat cacagcacca tcgacatttt cagacggcat cggtaaagaa
    12181 acccatctat gaacgcgttc aacctcacca tcatcggcga cgaaatctta cacggcagcc
    12241 gccaagacaa gcatttcgcc tttttcaaat ccctgctcga atccaaaggg ctgaagctca
    12301 atcaggtgca atatctgccc gacgaacccg atttgctggt caggcaactg cgccgcagct
    12361 tttcagacgg cataccgacg tttgttaccg gcggcatcgg cgccacgccc gacgaccaca
    12421 cgcgccaagc cgcagcggct gctttggatt tgcctgtcgt ccgccatcct gaagccgcca
    12481 agtttatcga aggcgtaacc cggaaacgcg gtgagccgct cgattcgccc gaacacgccc
    12541 aacgcctgaa gatggcggat tttccccaag gcgcggaatt ggtgcctaat ccgtttaata
    12601 atatcgccgg attttccatt cgcgagcatt atttcttccc tggttttcct gtgatggcgc
    12661 acccgatggc ggaatgggtt ttggatactt attacgtcgg ccgcttcaac caaaccaaac
    12721 gcggcagccg cagcgtgtat gtgtttgagc agcccgaatc gcgcattacg ccgcttatgg
    12781 agcatatcga gcaaacttat cccggtgtgc gttcgtacag cctgcccagc gtcggttgga
    12841 cgcattcaga cggcacgcag gtcaaaccgc acattgagtt cggcatcaaa gccgaaggag
    12901 aagccgtcaa ccttttggac gcggcgtggg cggaagtgtt gcacagcttg gacggattgg
    12961 gagcagagct gaaaaatcgg gtaaactgaa aaccgtttgc ccaaatcgga cgatatttaa
    13021 ggaaaaccat gcaataccga atcagaagag aaaacgaagc acccgaagca aaaaatacgg
    13081 gggaaacctt atgggagcgc gacatcatgc gcgaagtgct gctgtccgcc tatcaggaca
    13141 ggcgcaggga gcggatgtgg aaaaacatct ggcgtgccgt cagcaccctg attctggttg
    13201 ccctgattgc aggcattttc cgaaaagacg aagccgcatt gcagttggcg ggcaacacgc
    13261 cgcataccgc agtcgtcaac ctgtacggcg aaatcggcaa cggtgtagaa gaccaggtca
    13321 aaaaactcaa agacggtatg gaggccgcct acaaaaaccc gcaggcaaaa gccatcgtca
    13381 tccgcgccaa cagccccggc ggttcgcccg tcgtctccaa caccgctttt gaagaaatac
    13441 gccgtctgaa ggcgcagcac cccggcattc ccgtttatct cgtggcggaa gatatgtgcg
    13501 cgtccggctg ctactacatc gcggcggcgg cggacaaaat ctatgccgac ccgtccagca
    13561 tcgtcggcag catcggcgtt atcggcagca gtttcgacgc gaccggcctg atggaaaaaa
    13621 tcggcgtgaa acgcagggtt aaaattgcag gcagcaacaa aggcatgggc gatccgtttt
    13681 cgcccgaaac gcccgaacag tcgaaaatct gggaagaaat gctgaccggc atacacggcg
    13741 aattcatcaa agccgtcaaa accggacggg gcggccgcct caaattccga caatatcccg
    13801 acgtgttcag cggtcgcgtg tacacaggcg cggatgcgct gaaagtcggg ctggtggacg
    13861 gactcggcaa tatttacagc gtggcgcgag acgtggtcaa agcccccgat gtggtggatt
    13921 acactccgaa ggacgatttc ggcagaatct tgggcagacg cttcggcgca gaactcaaag
    13981 caagcgtgcg cgaagccctg caggccgtcc ggtagcgttg tttacagtat ttctaaggga
    14041 ataccaaaat aaaaatatac acaaaaacac tcacctcccc agtatgctgc cactaagtca
    14101 gccgcgtcac tatttaccgt gcacttaaag ccgtaaaagg caggttgtcc ccgggaggtt
    14161 ttgtgcatcc tgataccgat cggttgttgt tgctcaaagg acagaaggcc gctgataaac
    14221 gagattacct gtttgtcgct attgacgatt tttatactct gccattttgc cagacaaaac
    14281 cgcagacagt gctgccaagt ttctgaccga acatctggcc gacccctgct tgtacctgat
    14341 tgagtacgtt tactctgaca atgataggta atataaagag ccgtccaaca tgctttcggt
    14401 gcagcttgtt ataataatgg gattggttgg aggcttgccc gatttgcttg tccgcagacc
    14461 aacggtaagg cggagcgggt tatccgtacc ttgatggaga tgtggcatga ggaacagtcg
    14521 tttgacagtc cggaacatcg gcaaaaggag ttgtgctgct ttattaattt aggggctacc
    14581 ctagatcact agggaaattc aaattaagtt agaattatcc ctatgagaga aagtcgtcta
    14641 agccggtata aacaaaataa actcattttg ttatcgtggc tatgatgtat tggatgcggg
    14701 cgaatttagc catttccgta tcaatcacag cacacatttt gccgaacgac aaaaccatat
    14761 taatggaact gagaactttt ggaatcaagc aaaacgtcat ttacgcaagt ttaacggcat
    14821 tcccaaagcg cattttgagc tgtatttaaa ggagtgcgaa cgacgtttta acaacagtga
    14881 gataaaagtt cttgttccat tttaaaacaa ttagtaaaat caagtttgtc ctagttgtcc
    14941 agggcagcct catctcttta tagatagctg cttaccaagt atccttaatt gctaattcaa
    15001 gccaatcttc aaaactatta aaaaattcat ggttttctat attatctaaa ttaatgacaa
    15061 taaccctaga atttcctgtt acatcattac catcaaaata agcttcccat tctccattac
    15121 tagcgaatgg gataagattt aaatcaggca ggatagaatt tctaaatata taagctactt
    15181 ctgccccttc tgttccataa ttctcaaacc accagtgaaa attatgtggc tgaatataag
    15241 gtattaaaga tgtatcgtta gagatacgaa catacttttc aggataacaa aacctttcag
    15301 gaagcatatt actaggatat aaattaaatt cattttaaat aatcatttcg ttaccttagg
    15361 atcataaata ctaccattag gaaactgggt agaccaattt ggtggtgcta gttcattttc
    15421 aatttgtttt ttaattctaa attgaatagc ttctccccat gtttgcggca tctaatgctg
    15481 cttgctctgc atcaatatta ctaaaaattg gattagtctc aaaaatgaaa aattactgtt
    15541 ttttcatttt ttatagcata ttcaagtaca tgaataatat ttttcactaa gggctctgta
    15601 gaaaaatctg aattttgtaa tttttctttt acagccaaaa tactttcctt atctattagc
    15661 caatccccct cacttggtcc aaataaccaa ttatcggtaa tttcagaaac aagtctccat
    15721 aatccacgat tttgatattc aataatttga ctttccgtaa tttctacaac tgtttcagat
    15781 tgaatgtttt tataagccca atcaatcatt tcagtatatt cactaattga aacattgtct
    15841 tttaaacaga aaattaacag gttgtgttca ttcatttgtc taagttctta atatgagttt
    15901 gttgttgtaa ataatctttt gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg
    15961 gatgatgccg cgaatatcat ccatacggtt accccaccaa attttattgg tgtatcgtta
    16021 atcatttatt tcaaaaaagt tatttattat catattatca gggagcaggt cgttaaaaaa
    16081 aaacccctca aattccatta aattagtatt tatccagcac ataaacgaac caaagcgttc
    16141 tttccctgag tgtaaatatt taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt
    16201 taatggtttt tctatagtga aaaaaaagcc cttactcgtt ttagttcgag atactatttt
    16261 aaataaattt ttattttcaa taaaataaga atattcattt actaagttgt tatgataaaa
    16321 aagtaatttt attaaaatgt tttcataatt ttctaggttc atttctagct caatttaaaa
    16381 tgatttcatt gttgccgtac caatgcgaaa tttaccatca acaaaatttc ccttcactga
    16441 tacaggtacg ccatcaactt gggtagtttt attttcaggc tgcctgaaag aaagataggc
    16501 aacttattac tcatcttacg ctagccctca ataattgaat acgttgcact agaaccaatt
    16561 atttctatga atgtttgctc aaacataaaa atatagtatt taaagaattc tctattcaaa
    16621 ggaaaataac tttctttcaa tatccattcc gcataacact catcaagaag ttcaaaaaag
    16681 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
    16741 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
    16801 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
    16861 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
    16921 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
    16981 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ctatcaggta
    17041 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
    17101 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
    17161 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcacct
    17221 taccggtttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
    17281 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
    17341 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
    17401 agccggtatt tttaacccaa tcccatttag tcgggttaag ttctactttt ttacctctcc
    17461 aaaccgtacc tgcggcctct gctacggaaa gggcagtttg ggctgttgca gttatattcg
    17521 gatgggcatc agcccattgt ctggcagagt tgatactgtc ttttaccgca aaagcactgt
    17581 cttgtaatgc ggttgcggcc gcaagttgtg cttcgggact taaatttcct aaattattaa
    17641 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
    17701 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
    17761 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
    17821 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
    17881 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
    17941 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
    18001 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
    18061 atatccagaa tgatgacgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
    18121 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
    18181 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagcctcatc
    18241 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
    18301 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
    18361 cataggtcgg gcagcaggtt ttttatagcc ggtattttta acccaatccc atttggtcgg
    18421 gttaaggttt acttttttac cgccccaaac cgtacctgcg gcctctgcta tggcaagagc
    18481 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
    18541 gctgtctttt accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc
    18601 tggacttaat tttcctaaat cattaatacc ttgtagagtc tgctgagcgg ctgtatctga
    18661 gccggtcagt gtaggttgga ttaaaaccca gcattttcaa catcttgatg ttttactata
    18721 aatctattct tgtgcttcct ttgttagata aatttcaaca caaaagatac gtatgcattc
    18781 tctaatttta tttgagataa ataatgcatt gctttgtcga tactgttaaa taaattactt
    18841 ccctcgtaat accaattatc atatgtttga taaaaataat tatctttctt gatataaata
    18901 tctcccccca atacaagtat tctctcattg ataaataaat ttaatatatc tattgcaata
    18961 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
    19021 taaatttcaa aaatttcatt taacatgtta atctctatct actatttttc aatcggtata
    19081 aaccctattc acattttatt tttcctaaaa ccaaatcttc gactaactgg gttgcataaa
    19141 taatatcatt tatgatttta acaggtaaat tatttgatag gtaaccatgg gattccgtca
    19201 tcattgattt ctctaggtgc ttatttatct gattggtaag ccttttgcgt aagcttcttt
    19261 cgcctccgca agtgtcattt cattaggtcc accatctata ttagtttctc ctgtattttg
    19321 ccactgacaa acgtcacaga tatcatagtc cataacttca gttccgcaaa caggacaatg
    19381 tagccatatt tctccgtcaa tttcccatgt cttttttgaa ttctccatca taatacttct
    19441 ttcctaagtt tggtttaaac attgttttga ttttgaatgt ttttggattg gccaaaacat
    19501 agatattgtt attgatatca tagcgcactc gtctatgttc cgtctgataa cctaatatct
    19561 tgtccgatgt ctgttttgat aacaaatcag acgccatttt ttggtagtct tcaatggtta
    19621 tatcgccaaa ttcttttcca tgagctttga aatgtccatt taaagatttc tcagtaggaa
    19681 acttggactt tgtccatctg atgcggtctt gaattgttcc tgtcggagaa atagatgtat
    19741 caggtttaac atatgaggca ggtaagccga tattgttatc agagtgtgta gggtgttgct
    19801 gcgtacttgg ttttggtggc ttattcccac ctgccatctc cccatctaaa gtctgcatag
    19861 ggcgggcagt aggtgtttta tagccggtat ttttaaccca atcccatttg gtcgggttaa
    19921 gttctacttt tttaccgccc caaaccgtac ctgcggcctc tgctatggca agggcggttt
    19981 gggcagtagc tgtaatgttc ggatgggcat cagcccattg tctggcggaa ttgataccgt
    20041 cttttaccgc aaaagtactg tcctataatg cgcttgcggc agcaagttgt gctttcggac
    20101 ttaaatttcc taaatcatta ataccttgta aagtttgctg cgcagagatg taggtcggat
    20161 atttgtatcc gacaaaaata tttgacgcat ctatcgtttc cgaaatgccg ctgttggaaa
    20221 atgtcggatt cgagaatccg acctacggct atttcgttta agtcataaat agccattcat
    20281 gttccttctg aatttgcttt tgctatcctt agaaccaatc atataaacat tatcatcatt
    20341 aattaatgtt ctaagtttat gaagcggtca aatctgtcaa actaccagag atgaagcata
    20401 tcctcctgga agtaattctg ttcgtgctgt ttccatatct tctttgccat catcagttac
    20461 ttctctgtct aaataaaatc ttacgataag tttttgtcta atttcatcaa attctgccgt
    20521 aattaatctt atattgatat gaatacacgg ttcatacctg ctattagcca gtcaggtgta
    20581 gttggatggg gattcatttt taataatatc ttcaagcaag tgaaatgtaa gaaaagcgta
    20641 gatacttgaa ggccggctat ctgattggca aaaagaaatt tcggagataa agaatttccg
    20701 tttatatttt tcataaatta aatcaacacc ttgttttaca taaaaactaa cttcctcagg
    20761 aaggatattt ataacattct ctccttttac ttttatagtt aatggtttga tttttatgtt
    20821 ttcatgaata tctgatagga ttatttctaa aaaattattt ttatttccag tcattctggc
    20881 tattttatgt atatttccat tgtgttcgat tatcatttta ttttttccaa ggatttcctt
    20941 ctatattgat agtttatcta aagatttcat ttaaaatatc ctgactaatt tcttgtttat
    21001 ataattctac aattttctgt ttctgtgtat ttgtataaaa tttttcgttg gtatctgctt
    21061 gatattttaa ttgtctcaat atttccatga tttcatattc tgatttttca tcatattctt
    21121 caatcatgta atcaacaaat tgttttagaa tatcaaaggc ttcgtaactt tccaaaattt
    21181 taatatgttt atcggcaaaa ttggacatat accattcgtc agtgattgga ttagtattta
    21241 aatcatctaa aaaaacattt aaatcatcta aaaaaacatt tacatcatct aaaaacatga
    21301 ctaatcctta aacttaaaat ctgttttctc accggttctt gagttttcaa tgtaatgtat
    21361 ttcaattttg tttacgtttt gaacacgctt aacccaaccg tcttttgcta accaaccatt
    21421 tttagtatcg gacataggag gtattctcgc aggagttttg ccctgtgggt ttgccttaac
    21481 ttcttccatt gctaattgtt cctttaaatt attaggtatt gtacgaccgg ttgatttttc
    21541 aacagattgt gtccctattt ttaatgattt gtttccccca gccatttccc catctaaagt
    21601 ctgcataggg cgggcagcag gttttttata accggtattt ttaacccaat cccatttggt
    21661 cgggttaagg tttacttttt taccgcccca aaccgtacct gcggcctctg ctatggcaag
    21721 agcagtttgg gctgttgcag ttatattcgg atgggcatca gcccattgtc tggcggaatt
    21781 aatgccgtct tttaccgcaa aagcactgtc ctgtaatagg ctcgcggcag caagttgtgc
    21841 ttccggactt aaatttccta attcattaat atcttgtaaa gtttgctgcg cagagatgta
    21901 ggtcggatat ttgtatccga caaaaatatt tgacgcatct atcgtttccg aaatgccgct
    21961 gttggaaaat gtcggattcg agaatccgac ctacggctgc taccaaccat cattttcagc
    22021 ttcttttagc caagtatcaa aattttcaaa agtctcgtaa tattttgtat tatctaaatt
    22081 aactactata attttaggat tacctgattt atctgaaata tcaaaacagg cagcccactc
    22141 ttggtttcta gcaaaaggta ataggtttgg aatgccagtt atttcaaaat aaatgtcaat
    22201 tacttctgat atattgcttt ctgcattttc aaaccaccaa ggaaaagaat attgttcatc
    22261 gtagttaatg gcatgagtgg attgagctaa ttttaaataa ctttgcggat atttaaatcc
    22321 tttaggaagc attgcttcag gatacggaac aaaactattt tgttttatca tttcctaagt
    22381 acctttacat cataaatact tccgttagga aatcttttgc tccacccttc tggaactcct
    22441 ttttgtgttt cttgctttct aattcgtagc tggatagctt ctccccaagt ttgaggctca
    22501 ataccatcat gcttaaacct gattctctct tttgatgata atcggtcatt aaaattatga
    22561 acattcgctt ccacttttac tcctgcttca cgtgctgcca taatacgcgt gttatccata
    22621 ctagtaggtg ctccatcagg catattaaca acatcaaccc tgtcgccaac ccatgatttt
    22681 tttctttcat ttcggctaca atagaatcat agttgtaatt tgcactaggc tctgacaaaa
    22741 atttgggttg ttaagttgtc atttttttat aaattccagc aaactgttgt tgtaaattat
    22801 ctacttcatg ttttctaaca ccagatacat tttcaaattc tcttgaatct acacatacgc
    22861 ccgcatatat ctcattcaag atagctcgta aaacaaaaac ttcatcttga gttagtgaaa
    22921 tagttgagtt ttgattattt atggatagta tattcatggc tttttcctat aaatcatcat
    22981 aatatttttt acctgatgtt ggtctaaatg ctgtacctcc atcgtcagaa tttttatctc
    23041 ggataactat tgtccctgtt ttatcatccc aatacgcagt tctaccgcga ggtaactctt
    23101 tcatatttgt tggatggcta acaatatttt caatatgccg agcaaaatct gctggtgaat
    23161 tgatatttaa atccgtaaat tcttgttgtc ttatgacatg cttgttataa gcatgcccac
    23221 tactaatttg ttctccaatt agttgcgctt gtaaagacgg ttgtgtggaa gcatctgctt
    23281 tgctgttgga cgttatagat ttaggcggtc tattcccacc ggccatttcc ccatccaaag
    23341 tatgcatggt gcgaacagca ggtgttttat agccggtatt tttaacccaa tcccatttgg
    23401 tcgggttaag ttctactttt ttaccgcccc aaaccgtagt tgcggcctct gctacggcaa
    23461 gggcagtttg ggctgttgct gttatattcg gatgggcatc agcccattgt ctggcggaat
    23521 taatgccgtc ttttaccgca aaggcactgt cctgtaatag gctcgcggca gcaagttgtg
    23581 cttccggact taattttcct aaatcattaa taccttgtag agtctgctga gcggctgtat
    23641 ctgtgacagg gcttaccgca ctgtctgtaa ttgccccaat ccctactcct tgaaaaccca
    23701 ttaaaaaagg attaaccgca ccttggacga tgccgcgaat atcatccata cggttagccc
    23761 accaattttt attggggtcg ttttcccata tcagttttcc tgcttcatca gcacagttag
    23821 gattggcatt gatgcccacc cagtttcagg cagcctgaaa aggattttta atatacggca
    23881 aaaggtgggc aacaagtgcc caccttacct agctaacatt tatcccaagt atcatatatt
    23941 tttttaagtt ctttatccaa agaaagaact ttattttttc aacaccatga attatctcaa
    24001 aatctctaca atccacgcat acgcctgaat aaatttctcc tacaattgct tgaataatat
    24061 acaactcatc tttatcgaga ctaattttaa ctgaactctc ttcaaaatta attaatttca
    24121 tgaataattc cttttatttt aattcgtcat caaaataccg tttgcctgtt tttggtctaa
    24181 atgctgtcca ccatctttag tcttggatat ttagattata atcgtcaaat tcccagtcaa
    24241 aatattttgc caaatttgat atagacttat cttcagacgt tgaattacat atttccaata
    24301 tatcatttat caattttaat atgtgttcaa tattaaaacg gtttgtaaca attgagtttt
    24361 ttaatgacat cggtctatgt atatttgaaa gtaaccattc agtgctatat atataaacat
    24421 aaaaataatc agcaccatta ctttcatcaa aacctattga taatgtcaaa agtatacaaa
    24481 aattttctaa cttgtcttgt gggaaatctt ccaaatcaaa gtcagaacaa tcataatttt
    24541 ttaattttaa tctcatataa ttacctaata tttaaatgtc catttttaat tttattcctt
    24601 tttccatttg aatcaatagt ataagtttca aaatttgctt gaatacctgt agttgcaaat
    24661 tgtgaatttt tttctgttgg tggccgatat tgtcgagtgc catctatact taaccatccg
    24721 cctccactag tttgtcttgc accctcacca acccaaattt tacctagcct atctgcctct
    24781 tcataagttg cagttcctat tggaaaattt ttttcactct tatgaagagc tagactcaat
    24841 cttggatctt gagccgcaat gttttttaag ttttgcccag ttaattgatt aactaattga
    24901 ggataagttg cagcattggc ttttcctcca gacgttatag atttaggcgg tttattccca
    24961 ccggccattt ccccatctaa agtctgcata ggtcgggcag caggtgtttt ataatcggta
    25021 tttttaaccc aatcccattt agtcgggtta agttctactt ttttaccgcc ccaaaccgta
    25081 cctgcggcct ctgctatggc aagggcagtt tgggctgttg cagttatatt cggatgggca
    25141 tcagcccatt gtctggcaga gttgatactg tcttttaccg caaaagcact gtcctgtaat
    25201 aggctcacgg cggcaagttg tgcttccgga cttaaatttc ctaaatcatt aataccttgt
    25261 agagtctgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat atttgacgca
    25321 tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat tcgagaatct gacctacggc
    25381 tgctgactat tttcttgttt atataattct acaactgcaa actactatca aatcttttta
    25441 tctctggctc tacactcagc aagaatattt ttattttaca aaacataggc tagttatttg
    25501 agtatattgt caaattctaa tttgtttttt tgatagattt tttttatacc attaatacat
    25561 tcattagttg taattctacc aagcgaatgt aatttatcaa tatcaaaaca atcggtataa
    25621 accctattca cagttttatc ttgtatttcc ttatgaatac ttaatatatt gagatagttt
    25681 agaaatttta ttttctcaga ttgaggtaac tcatcaaaac caagccaata ataaaaattg
    25741 ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc ttcaactaac
    25801 tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga tgggtaacca
    25861 tggggttccg tcatcatttg taactcctgg tacatgtgcg tgtcctgtaa gtgcgcgtgg
    25921 gtcttgatgg gtatttgcat gatgagcatc atctattctg gctccagtca agtttccatt
    25981 actatctcgg acttggataa aacgagcatc aggcgaacct gttatataac cgcctattgg
    26041 tgttggtatt gcatttcctt tgctgtctac ataaattgtt cctgtcggag aaatagatgt
    26101 atcaggttta acatatgggg caggtaagcc tatattgtta tcagagtgtg tagagtgttg
    26161 ctgcgtactt ggtttcggtg gcttatttcc cccagccatc tccccgtcta cagtctgcat
    26221 aaggcgaaca gcaggttttt tatagccgtt atttttaacc caatcccatt tggtcgggtt
    26281 aagttctact tttttacctc tccaaaccgt acctgcagcc tctgctacgg aaagggcagt
    26341 ttgggcagta gctgtaatgt tcggatgggc atcagcccat tgtctggcag agttgatact
    26401 gtcttttacc gcaaaagcac tgtcttgtaa tgcggttgcg gccgcaagtt gtgcttccgg
    26461 acttaaattt cctaaattgt gaataccctg taaagttttc cgtgctgccg cataggttac
    26521 cgggtttacc gcactgtctg taattgcccc aactcccaat ccctgaaaac ccattaaaaa
    26581 aggattaacc gcaccttgga cgatgccgcg aatatcatcc atacggttag cccaccaatt
    26641 tttattgggg tcgctttccc atatcagttt tcctgcttca tccgcacggc tgaaaaaacc
    26701 agaggcggca ccggcatttt cttttagcca acggtctgaa aatggggctt gagggacaat
    26761 attagtcttt gtttttgttg aagttccttt gacataatag ctgtatatat cccttgctcc
    26821 tccggggggc ggataatcgc tgccttgcgg cccgtcatat ccatcctccg gatgaatttc
    26881 cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg tctacaccgc cgctgaaatc
    26941 agaagtgctt tttgaatcat gattatcgaa cggactgtgt acttcatgtc catgtcctga
    27001 aaaatgggtt tcataaccga taatgccttc aaaccctgtc cgttcgtgtg taataggcag
    27061 tacaggactg accgcagttg catcaaatgt ctggacggcg taaacccgat ttttaacact
    27121 gccgcgagca ttaccaaaca gatggtattt accacccggt tcgtagtgtt ttgcctgcat
    27181 atcatcgcgt aaacgggcat ccaaaccgtt cgcatgacta atggggattt gcagcaacga
    27241 tattgccgct gcaaacagca tcatgaattt ttgaataggc aatttcattt tgttttcctt
    27301 ggtagcaagc ggttatggtc gtgtgaatca aggttgccct tgtctatgtt gtcgcactac
    27361 ttcatcgctg tatccatacc cctcatgact gttatcagtc tctacggatg gggcggagtt
    27421 acccgtatga ttgccgtatg gtcggatatc ggagaaatcg accattaatc cttccgtcgg
    27481 tttaattcct ttgcttactt tatacggccc catccacaat gcgtaatttt ctttataggc
    27541 agcttcaaac gcattggttt tgggtttgat gagcaatttt ttattggttc tgtctactgc
    27601 gaaatattcc agttttgttt gggctttcag tgtttcggca ttgtataggt gcatttcggt
    27661 tctgttgcgt atcgttccga atacgtcgat gttaataaac acatctgtat cggcattggc
    27721 aggagaaaca acgtctatgc cgcgcaggaa aaatacggtc tgtaccaagt gggaaagaaa
    27781 ggcagtgtcg cgcgggttag tcgtcaaggt ttcatttcga taatccccca tcccgccaat
    27841 atttaagccc agactgctcc tacttccgct accgtctgat tgggtgcgcg agcgtgcagg
    27901 ggcattaagt gtagataaag aagtggttaa acctgtcaaa ccgcctgatg ttgtttcagc
    27961 ggtggtttcg taacgtggat aggtgtaatc ggtacggacg gcagggctgt ttatgtattc
    28021 gccacgaatc agtgcatcaa tggagtagcg accccctgtc aaactgcctg aaccttggtc
    28081 gcccatagtt gcaatgtaca atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc
    28141 tttaacggca gctctggcag aagcggccac aagttcttgt tcgaccgcaa agcgtttacc
    28201 tccgccatgc gatggaatac ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa
    28261 aagaataggt atcagcagcc gtgcttgcat agattttctc ctttgatgaa aaacaaattg
    28321 tatcaaaatt gtaaatatag tggattaaat ttaaaccagt acggcaaggc gaggcaacgc
    28381 cgtactggtt taaatttaat ccactataat ctccatataa taaatggctg tctgaagccc
    28441 atatgcgact atatctgccg acactatggt caatcagcag tgaaccagcg taccttcgtt
    28501 ttcaccggtt actacacgtt tgagcgagcc ttctttagca atgccgaata ctacaatatt
    28561 gagcttgcgt tcgcggcaga gggcgaaagc ggtggcatcc atcactttga ggtttttcaa
    28621 caaggcttcg tcaaaagtaa tggtttcgta gcgcgtggcg gatgagtctt ttttggggtc
    28681 tgcggtgtac acgccgtcaa cgttggtggc tttgagcatc acgtcgcagt tcatttccgc
    28741 accgcgcaat gcggcggcag tgtcggtcgt gaagaacggg ttaccggtac cggcggcaaa
    28801 aatcacgact ttgccttctt ccaaatattg gatggcttta ggacgggcgt aagtttcagc
    28861 gatttgctgc atagacagtg cggattgtac gcgcgctttg atgcctaaag tttcaaatgc
    28921 gtctttgagt gccaacgcgt tcatcacggt cgccatcatg cccatgtagt cggcggtggc
    28981 gcgatccatg ctgcctgctt gggcggatac gccccggaaa atattgccgc cgccgacaac
    29041 aataccgact tgcacgccca ttttaacgac ttcggcaatt tcgccgacag tttgaacgat
    29101 ggtatcgtga ttgatgccga acggatcgga acccatcagg gattcgccgg agagtttcag
    29161 taatacgcgt ttgtatttga tttgctgtgt catgggatac cttgctttct tgaatgtcgt
    29221 cggtcggagt ttgggccgtc tgaattttca aacggtcgga attacaatga tatggtgtgt
    29281 cgttctgtta tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
    29341 aaatagtacg gcaaggcgag gcaacgctgt actggtttaa atttaatcca ctatattgtt
    29401 ttgttttcag acggccttta tattcggtcg tcttaaaata ggtaccgcaa acggttttct
    29461 caaaaaaaag caccctgatt cgtttggaat ccaggtgctt tctttttcat aagtgcctta
    29521 cactttggcg gctgcggcta cttcggctgc gtagtcgaca acggctttct cgataccgtc
    29581 gcctactttg tagcggacga agctgaccac ttcagtgccg ttttctttgg cgaattgggc
    29641 aacagtttga tcggggttca tcacgaatgc ttggccgttc agagtgattt cagccaagaa
    29701 tttgcggata cggccttcaa ccattttagc ggcgatgtcg gcaggtttgc cggaagcgat
    29761 ggcttgctcg gtgtagatgt ggcgttcttt ttcaacggtt tcggcatcta cttcggcttc
    29821 gcttacgcat tgtggtttag cggcaacaat atgcataccg attttgcgtg ctacgtcttc
    29881 agagcctttg tactcaacca atacgccttc ggtcgccaat gcgccgtgga tgtaggcaac
    29941 cagttggttg gcagtgtcga tcacttggaa gcgacggaca gacatattct cgcccaattt
    30001 ggcgatgatg gctttgcgtt ctgcttcaac cagttcgctc agttcttcaa cagaagccgg
    30061 ttttttctcg gcagcagttt tcgcaacgaa gttggcaaat tctacgaagc ccgcgtcttt
    30121 agcaacgaag tcggtttcgc agtttacttc gaccaatgcg ccgacattgc cgttgatcgc
    30181 gtaagccaat acgccttcgg cagcggtacg gccggccagt ttaccggctt tcgcaccgga
    30241 tttgatacgc aggatttctt cggctttgtc gaagttgcct tcggcttcaa ccaaggcttt
    30301 tttgcattcc atcatgccca ggccggtagc ggcgcgcagg tcggcaacca tttttgcagt
    30361 aatttctgcc attttgaatc tcctagattt ttgggaaata cggaaaggat tttccgcatt
    30421 cgggaatcgt gtgccgcgaa cggacgttca gacggcatat ttgagaaaag gggcataacg
    30481 cccctcttcg gtttgccgga ttactcggca gcggcttctt gggcagcggc tacggtttct
    30541 tgcagcgctt ggtttttgcc ttccaaaact gcgtcagcga tgccgcggca gtacaggcgg
    30601 atggctttgg cggagtcgtc gttgccgggg atgacgtatt tcacgccgtc agggctgttg
    30661 ttggtatcga ctacggcgat aacggggatg cccagttttt cagcctcgac cagagtacct
    30721 ttttggtagc cggtatcgat aacgaaaatc gcgtcgggca ggcctttcat gtttttgata
    30781 ccgcccaaag aacgttccag tttttcaaca tcgcgttgca tttccagaat ttcttttttg
    30841 ctgaaaccgc tttcggcagc attttccaag gctgcggttt tttcttccag gcgtttgatg
    30901 gattgcttaa cggttttgta gttggtcagc ataccgccca accaacggtg atcgacgaaa
    30961 ggcatgccgg cgcgggtcgc ttcttcgcgg atgatgtcgc gggcttggcg tttggtacct
    31021 acgaacaata ctgtaccttt gttggcaacc agacgacgta cggcttcttg cgcgtcttgg
    31081 aacatcggca gggttttttc caggttgacg atatggattt tgttgcgcgc accgaaaatg
    31141 tattgagcca ttttcgggtt ccagaaacgg gtttggtggc cgaagtggac accggcttca
    31201 atcatctgac gcatagtaat ttgagacatt ttgatttcct tgaaagggtt aagggcacac
    31261 attcaatcgc atagaacttg atagaccgcg cctattgtca agcacccttc ggcgaaagtg
    31321 ggcataagtt ttaaaaaata aaagagtgtt tccgaaatgg aaaactgacg gattatagcg
    31381 gataagaggt cgtctgaaaa gggattcagg gttttgtgcc gctgtctttt taccgttgtg
    31441 cacgctgctg ttcgaattct gcctgttcct gttgcttcat caggcggttg cggcggcgga
    31501 tggtcacgac aaagaccgtg aacacggtgg gcagcaccgc ccaaaaaacc aaataaatca
    31561 aggcgcgcgc aatactcggc tgcgcggcgg aaaacatcac ggcaacaaaa agatagccga
    31621 tggcgacaat gtgccacatt gtgtgcgtcc tgtttgaatg ttaacaaaat cgttataatc
    31681 gcaacattct accgcaatcc tgaaaggccg tctgaaatga ggaacgagga aaaacacgcc
    31741 ctgcgccgcg aattacgcgg gcggcgttcg caaatggggt gggacgtgcg ggcggcggca
    31801 acggtaaaaa ttaaccgcct gctcaaacgt tatatcaagc acggtcggaa aatcggcgtg
    31861 tattggccga tgggcaagga attgcgtttg gacggctttg tccgcgcggc gcaaaaacgc
    31921 ggcgcaaaac tttatctgcc ttatatcgaa ccgcgttcgc ggcggatgtg gtttacgccg
    31981 tatcctgaaa gcggaatgga acgggagcgc atacggggca gggcgaggtt gaacgtgccg
    32041 cagtttgcag ggcgcaaaat ccgcgtgcac ggtttgtcgg tattgctcgt tccgcttgtc
    32101 ggcatagacc gcgaaggcta ccgtttgggg caggcaggcg gctattacga tgcgacgctt
    32161 gcagcgatga aataccgttt gcaggcaaaa accgtgggcg tgggctttgc ctgccagttg
    32221 gtggacagac tgtcggtcga ggcgcacgac ctgccgctgg acggttttgt gtcggaggcg
    32281 gggatattgt gcttttagcc cttcagacgg catttcgggc ggaaatgccg tctgaaggcg
    32341 gaatattggg agaaaaggat gtcggaaagg ataatactgc cggtcttgtc attgggcggc
    32401 gttcggctcg aaccgttgga tgtgcatcat gaaacgggtt tgcgcgaggc ggtttgcgat
    32461 ggggaggttt ggaagctggg ggtaacgtcc gcgccccatc cggatagggt ggcagactat
    32521 atcgggacag cattggcaac gcgtttggcg tttgcggttg tcgatgaaga ggcgggcagg
    32581 gtggtcggga caacggcgta ttatcatttt gaaccgcaga ttccgcgttt ggatatcgga
    32641 ttcacatggt atgcggcatc ggcacggcgg acacggataa acacctgttg caaaatcatg
    32701 ctgttggatt acgcatttga tgttttggct tgccgttgcg tgggatggcg cacggatatt
    32761 ctgaaccttg cctcgcaacg tgccatcgaa cggctgggtg cggaaaaaga cggcgtgctg
    32821 cgtatgcata tgctccgtaa agacggcagt gtgcgcgata cggtcgtgta cagtatgctg
    32881 agggaagatt ggtgcaaaaa cagggaaatc ctgaccggca ggttggcagg gtatggcgtg
    32941 caggtgtgag gcagtatttt caaat
//
LOCUS       DAWFIT010000012        32382 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFIT010000012 DAWFIT010000000
VERSION     DAWFIT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.012"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(98..844)
                     /locus_tag="WGQ85_000547"
     CDS             complement(98..844)
                     /locus_tag="WGQ85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0750032.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1023..1694)
                     /locus_tag="WGQ85_000548"
     CDS             complement(1023..1694)
                     /locus_tag="WGQ85_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0750033.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGQ85_000549"
     CDS             complement(1763..2434)
                     /gene="rpiA"
                     /locus_tag="WGQ85_000549"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0750034.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2511..2993)
                     /locus_tag="WGQ85_000550"
     CDS             complement(2511..2993)
                     /locus_tag="WGQ85_000550"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0750035.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3025..3714)
                     /locus_tag="WGQ85_000551"
     CDS             complement(3025..3714)
                     /locus_tag="WGQ85_000551"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0750036.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGQ85_000552"
     CDS             complement(3711..4445)
                     /gene="dnaQ"
                     /locus_tag="WGQ85_000552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0750037.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4601..5911)
                     /locus_tag="WGQ85_000553"
     CDS             complement(4601..5911)
                     /locus_tag="WGQ85_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0750038.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGQ85_000554"
     CDS             complement(5901..6086)
                     /gene="ccoS"
                     /locus_tag="WGQ85_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0750039.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6086..6385)
                     /locus_tag="WGQ85_000555"
     CDS             complement(6086..6385)
                     /locus_tag="WGQ85_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750040.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6736..7932
                     /locus_tag="WGQ85_000556"
     CDS             6736..7932
                     /locus_tag="WGQ85_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0750041.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8568..10409)
                     /locus_tag="WGQ85_000557"
     CDS             complement(8568..10409)
                     /locus_tag="WGQ85_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0750042.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10500..10970
                     /locus_tag="WGQ85_000558"
     CDS             10500..10970
                     /locus_tag="WGQ85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0750043.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGQ85_000559"
     CDS             complement(11041..11853)
                     /gene="hpnC"
                     /locus_tag="WGQ85_000559"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0750044.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11940..12422)
                     /locus_tag="WGQ85_000560"
     CDS             complement(11940..12422)
                     /locus_tag="WGQ85_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0750045.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHYHH
                     "
     gene            12895..13101
                     /locus_tag="WGQ85_000561"
     CDS             12895..13101
                     /locus_tag="WGQ85_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750046.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAAATEAPAAEAPAAEAAK"
     gene            13211..14578
                     /locus_tag="WGQ85_000562"
     CDS             13211..14578
                     /locus_tag="WGQ85_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0750047.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14759..16738)
                     /locus_tag="WGQ85_000563"
     CDS             complement(14759..16738)
                     /locus_tag="WGQ85_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0750048.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGNSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLAGWEEHLYSGLDPFHYITVTDNSHVIGQTISLDQ
                     FILENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDPKEAKN
                     TALIFEKNVTGTSNIIFENPIDDLKSLDGHQIIKVNGTADKRAFRLSGKHQKGIYTLS
                     LQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDKNSDIFDRTLPRKGLWLRLI
                     SGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLSVGLMGGQAEQRSTFRNPDT
                     DNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSKGI
                     TASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGKFSDSENAHVNLLGSRQL
                     QTRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEMDGERRVINNKTAIESQLGV
                     AVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17157..17603)
                     /gene="smpB"
                     /locus_tag="WGQ85_000564"
     CDS             complement(17157..17603)
                     /gene="smpB"
                     /locus_tag="WGQ85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0750049.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17658..18668)
                     /gene="waaF"
                     /locus_tag="WGQ85_000565"
     CDS             complement(17658..18668)
                     /gene="waaF"
                     /locus_tag="WGQ85_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0750050.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18799..18874)
                     /locus_tag="WGQ85_000566"
     tRNA            complement(18799..18874)
                     /locus_tag="WGQ85_000566"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18839..18841),aa:Asn,seq:gtt)
     gene            complement(19065..19817)
                     /locus_tag="WGQ85_000567"
     CDS             complement(19065..19817)
                     /locus_tag="WGQ85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0750051.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20128..20895
                     /locus_tag="WGQ85_000568"
     CDS             20128..20895
                     /locus_tag="WGQ85_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0750052.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKPLRQHRRRPVRSHIGGN
                     QFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRRIVRHFFIRQHRRRIIKRRAHPPG
                     VRQLHRIAHCLRRPVLCGEPFYGFLFKSNPLRLIEAV"
     gene            complement(20844..21123)
                     /locus_tag="WGQ85_000569"
                     /pseudo
     CDS             complement(20844..21123)
                     /locus_tag="WGQ85_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(21449..21610)
                     /locus_tag="WGQ85_000570"
     CDS             complement(21449..21610)
                     /locus_tag="WGQ85_000570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750053.1"
                     /translation="MPSEKKMGCRIWMSDHLIRPNVGLRYSGLNSNQYGVASPCRTIC
                     TVCGFVALS"
     gene            complement(21600..22745)
                     /gene="dapE"
                     /locus_tag="WGQ85_000571"
     CDS             complement(21600..22745)
                     /gene="dapE"
                     /locus_tag="WGQ85_000571"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0750054.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQQLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVGKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGSSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22859..23479)
                     /locus_tag="WGQ85_000572"
     CDS             complement(22859..23479)
                     /locus_tag="WGQ85_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0750055.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23513..24022)
                     /locus_tag="WGQ85_000573"
     CDS             complement(23513..24022)
                     /locus_tag="WGQ85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0750056.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIARYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(24116..24667)
                     /gene="azu"
                     /locus_tag="WGQ85_000574"
     CDS             complement(24116..24667)
                     /gene="azu"
                     /locus_tag="WGQ85_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0750057.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKITLVD"
     gene            complement(24948..25370)
                     /locus_tag="WGQ85_000575"
     CDS             complement(24948..25370)
                     /locus_tag="WGQ85_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0750058.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25501..28251
                     /gene="secA"
                     /locus_tag="WGQ85_000576"
     CDS             25501..28251
                     /gene="secA"
                     /locus_tag="WGQ85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0750059.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTNQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28266..28418
                     /locus_tag="WGQ85_000577"
     CDS             28266..28418
                     /locus_tag="WGQ85_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750060.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            28396..30168
                     /locus_tag="WGQ85_000578"
     CDS             28396..30168
                     /locus_tag="WGQ85_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0750061.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKKQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30355..32283
                     /gene="rpoD"
                     /locus_tag="WGQ85_000579"
     CDS             30355..32283
                     /gene="rpoD"
                     /locus_tag="WGQ85_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0750062.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         8211 a         9154 c         7619 g         7398 t
ORIGIN      
        1 caacgccgta ccggtttttg ttaatccacg ataaatttgc cacaaaaaag ctgcctcaaa
       61 tgaatacccg ggcagctttt tgttgatatg actccaatca gcggtgttgc ggattgtaac
      121 gtttttccaa acgcaggaat atccagccta agaaagtcgt catcaacaga taaatcaggg
      181 cgacggtgta aagcggttct tcataaaccg aataccggcc cgtaatcgta ttctgaacat
      241 acgccaactc cgccacagca atgaccgaca gcagcgagct gtctttcaag agcgtgatga
      301 actcgctcgc caaaggcggc agcatgcggc gcaatgcctg cggcagaatc acatagcgca
      361 tcgcctgcgg ataggtcagc cccaaagaac gcgccgcctc catctgtcct ttgtctatag
      421 actggatgcc cgcgcggaaa atctcacaga tatacgcccc cgagttggcg atcagtgcca
      481 aagaaccggc aatcagcggc ccgtatccgc gacgcagcgc gattgccgcc tcgccgctga
      541 ccaaaatgcc gtctgaagga tggacgaaaa acggaaacca cacatacgcc caaatcacaa
      601 tctgcacaaa cagcggcgta ccccggaaca gcgtaacata cagcagcgaa actttacgca
      661 acgcccacgc cagcacgcgc atcggcgcac cggctttttc caagtgaatc aggcgcgcca
      721 acgccaacaa cagacccaat accgaaccgc ccgccgtcgc cacgaccgtc agccccaagg
      781 tcgtcagtgc gccgtaaagg aacatccagc ggtattcgta aataatgtca aaacgaaaat
      841 ccatataccg tccgtatcaa aaaccggcgg aactgccgcc gttgcaaaat aatccgccat
      901 tttaccgtaa aaaccgccgc ctgaactttt ttatcgcggc agacggcggt tgcgcgtctc
      961 cgcaaaaatg cagggcgcgc ggttttcaga cggcatttgc cgttcaaagc cgtgcgctgt
     1021 ctttaccaaa tgcccaacca ttcgcccacg gcatccatcc aatccttatt gcccccgccg
     1081 ctcctgcctg cccggcggta cgcccacggc gcttgcggat ttttagcttt ccacaatcct
     1141 ttgcgttccc tttccgcctg aatttgagcg tcggcataat cggcaaaatc cgccttatcc
     1201 tgctgttctt tagcataact tttataatgc cacgccgccc cgtcctgcac ctgcatcagg
     1261 ttcaaatcgg ttttgccgac agaaacctgc gccacttcgc gctggtagcg gtcggtgtcg
     1321 aacacgcgca cgctgacttt cctgccttcc gccgccgcac gcaggttgtc gcgcgaacgc
     1381 gtgccgtaag cctgtttcat ctccggcgcg tcgatatacg ccatccggat tttgtgtttc
     1441 gcgccgtcgc cgtcgataac gtgaagggtg tcgccgtcat agactttgga caccgtgcct
     1501 gtgtagcggt ggccggattt cgccgatgct cggcggcggg cgggcgcgtc ggaacccgcg
     1561 tcccctgccg cgccgagtac gtcgagtacg gcaaccgccg tccgcaccgc ctcgctgccg
     1621 taccccgtat aacccaacgc acccaaaagc gacaaggcga cgggaagcca tttcatgatt
     1681 tttttaatct gcatattttt caaatgccga tgccgtctga acatatcgga atcggatttc
     1741 agacggcatc ttaacgtcag gattaccctt ggcagggata gatgactttc gcaccctctt
     1801 ccgtccccaa aatcaacaca tcggcggcat cgcgggcgaa tatgccgttt tcgagcacgc
     1861 cggtgatttt gttgatttcg tcttccatcg tcagcggctg atcgatattc aagccgtgga
     1921 catcgacgat ttggttgccg taaaacgtgg tgtagccgat acgcagttcg ggctgtccgc
     1981 ccatagcgag cagtttgcgc gaaacaagag agcgcgcgct ttcgacgact tccacaggca
     2041 gagggaattt gcccaaacgt gaaacatatt tgctttcatc cgcaatgcag acgaattttt
     2101 cggatgcgct ggcgacgatt ttttcgttga ggtgtgcgcc gccgccgcct ttaatcattt
     2161 gcagggcgtg gttcacttca tccgcaccgt caatatagac cgccaacccc gatacttcgt
     2221 tcagggaaac gacgggaata tcatattggg caagcagttc actggatttt ttggaagtag
     2281 ataccgcgcc tttgattttt ttgccgctct tacccaaggc ttcgatgaaa aagttgatgg
     2341 tcgaaccggt accgatgccg atgtattcgt tttcgggtac gaattcgact gctttttcgg
     2401 cggcgatgcg cttgagttcg tcttgtgtcg tcatattttt gtcctttggg aaaccgtatc
     2461 aacaaacagc cgccatctta acattttttt gcacgtcctg cccgccgcgt tcaaatcctt
     2521 accagcaata ccgccgcctg cgcctctatg ccttccatgc gcccgagata accgagtttt
     2581 tcgttggttt tgcctttgat gttgacgcac gaaatgtcta tgcccaaatc ggcggcgatg
     2641 ttggcacgca tttgcggaat atgcggcgcg agtttgggtt tctgtgcaat cacggtcgta
     2701 tcgacattga ccgcctgcca accctgcgcc tgaacgcttt gatacgccgc acgcaaaagg
     2761 acgcggctgt ccgcatcttt gaactctgcg gcggtgtcgg ggaaatggct gccgatatcg
     2821 cccaaacctg ccgcaccgag cagcgcgtcg gtaacagcgt gcagcagcgc atcggcatcg
     2881 gagtgtccga gcagcccttt ttcaaatggg atttcaactc cgccaagtat cagctttctg
     2941 ccttcggtca gttggtggac atcgtagccc tgtccgatac ggatgttcgt catcgtttgt
     3001 gttcctgatg ttttgaattg aagttcagac ggcattgagc agcagcctga cgatgtatgc
     3061 gtcctgcggc tgcgtcagtt tcaaattgcg cgcgtcgccc tgcaccagca aagggcggat
     3121 gcccaatttt tccacggcgg acgcttcatc ggtaatgccg tccaagtttt ccgcagccaa
     3181 tgcgcggtgc agcagccccg cgcggaaaag ctgcggcgtt tgtgcctgcc aaaggctcgt
     3241 ccgctcgaca gtggcactga tttgtccgcc ttctgcgcgt ttgagcgtat cggcaatgga
     3301 aattgccaaa atcccgcctt cggcggcgtt gcccgcctgt tctatcaacc gcgtcaaagc
     3361 ttcagacggc aggcagcagc gcgcggcatc gtgtaccaga atattgtcat ccgccgatgc
     3421 caagccgatt tccaacagtt ttgccacacc gtttcggacg gtttcggcgc gggtctgtcc
     3481 gccgtttttc cacacccgaa cctgtggaaa tgccgtctga accttatcgg caaacgtgtc
     3541 ttcgggcgag acgacaacga cggtcaaatc gacggcctca tgccgttcaa aaatcccaat
     3601 cgtatgttct aaaacggttt tgcttccgat ttcgacatat tgcttgggtt tgtccgcacc
     3661 gaaacgcgcc ccgatgccgg cggcgggaat cagcgcgata tttttgcgct tcatgcgtcc
     3721 gtcccgccgt tttcagacgg cacggcttcc ttgcgccaga tacaggcttc gcccaagccg
     3781 tccaaatatt gcccgtgcgc cgccaactcg ttttcgtccg ccctgatgac tttcagtttg
     3841 ccgctgcgtt tggtttcggt atgcaccacg ggtttggttt ccattttttc ctctgcggcc
     3901 gcacccatca ggtcgaactg ccgccgcgtc atagcaagat agacttcgcc caaaagttcg
     3961 cagtcgatca atgcgccgtg caggacgcgc ttgctgcggt cgacggaaaa acggttgcac
     4021 aaggcatcca ggctggcttt ctgcccgggg aacatttcgc gcgccatcgc cagggtatcg
     4081 gtaacggtac agccgagttc ctcaacggtc ggcaacccca tccggcggaa ctccatattg
     4141 aggaagccca cgtcgaattt ggcattgtgg ataatcagtt ccgcaccgcg caggaaatcg
     4201 gcaatctgcc tgccgacctc tgcaaacggc ggcgcgtttt tcccttccaa aacctgtatc
     4261 gtcaagccgt ggacgcgtgc cgcctcttcg ggcatatcgc gctcggggtg gacatagagg
     4321 tgcaggtttt tgtcggtcat ttggcggttg accatttcca aaccggcaaa ctcgaccaag
     4381 cggtcgccgc cgtcggcata cagaccggtg gtttcggtat cgaggatgat ttggcgtgtc
     4441 gtcatatcgg tgtctttctt ctatcttcgt aaattgctta ttttttaagc aatgtatttt
     4501 tctgttttca tttcaatgca caaacccact tattcacagt gtgttcacaa cattgggcag
     4561 gcggattgtg tattttgggg acaatttttt cagacggcat tcaaggtttt ttcctgattg
     4621 ccgccgcgcc taaaaaccgc ctttcgcgct taatcaaaaa taccgacaac ggaatattgc
     4681 ccaaagcgac aatcagatac aacaaggaaa tgctgtcaaa caaaaacagc aacaccgcgc
     4741 tcaaaacggc agcggaaacc ataaaaatac cgttaacgat attgttggcg gcaacggcgc
     4801 gggcgcggaa agtctcgcta ctggcggttt gcagccaggt atagagcgga acggagaaaa
     4861 atccgccgaa aaagccgatc agcgtcatca ccgccatcac gggatatgcc catccttgcg
     4921 ataaaaacca aaaaatgccg ttcagccctt caaaacggtg tccgtgcgtc agccacacca
     4981 aaaccaggcc gcaaaccgtc aaacccaacg caccaaccgt tacccaagcc aacatcaggc
     5041 gttccctgct gaacttggca cacagtaccg aaccggcggc aataccgatg gaaaacagag
     5101 caagcatcag gttgaaaaca ttgtcgttgc cgcccagatg gatttgggta aaggtcggca
     5161 gttgcgtggt ataaaccgcg ccgacaaacc aaaaccacga aataccgata atggcggtaa
     5221 aaacgggctt gtgccgcacc gtttcacgca gcagggattt tgtgccacgg acaatattcc
     5281 actcaatttg tgtatcggca gccttggcgg gtacggacgg cataaacagg ctgccgaccg
     5341 tgcctccgac ggcgaccagc aaaaccagta tcccgacaat ataaggcggt acacctgcca
     5401 ccgccgttcc caaaatctga ccgaacagga tggcgacaaa cgtacccgat tcaatcaggc
     5461 tgttgcccat catcaactct ttgtcgtcga gataatcggg caggatggcg tatttcagcg
     5521 gcccgaacag cgtcgattgc gcgcccatgc aaaacagaca cgccaaaagc agcggggcag
     5581 accggatata aaacccgtat gccgccaccg ccataatgat catttccagc accttgaccc
     5641 aacgcgccaa aacggccttg tcgaatttgt tacccaactg ccccgacagc gaggaaaaca
     5701 ggaaatacgg caaaataaac agcaacgcgc ccaagttcaa catctgtccg gcaggcagga
     5761 agccgttttg ccccaaaccg taaaacccaa tcatcacaaa cagcgcggtt ttgaacacat
     5821 tgtcgttgaa cgcgccgaga aactgcgtag cgaaaagagg tgcgaaacgg cggcttttaa
     5881 ccagtcccaa accgcctttt ttagcgtaca tcgttttccc tctctttttc aatcagttta
     5941 cttgtcgaat catcatccat caggatgcgg tgcgccggcc cttccaagtc gtcaaactgc
     6001 ccgtttttgc ccgaccacca aaaaaaccag ccgatgacaa acgccaaaat aatgctgatg
     6061 ggcaccaata taaacatgct ttccatcaca tattccctgt caaatcgttc aaaacaaaag
     6121 tctgccccga cacggtcaga tattcgttac gcaaagttcc gacgggagct tcgtcaaaaa
     6181 acagctcgat acggtctttg accacgcgcc aatattgggg gatttccgtc tgaccgaacg
     6241 gcgacaggac atgattttcc attccgcctt caagtttgac ggcaaaacgc ccgctttgcg
     6301 gccgtgcttc cgattcgtcg tcggcaagca ggatgaaaaa gcctatatgc cgtcccgatt
     6361 ggtcatgaat actgaaataa tgcataaatt tcccacccgc cttttttcag acgacaccaa
     6421 ctaaaaacag ggcgaatgta ccagtttgga cgggaagaat gcaaagaaat tctccctccc
     6481 ccagccgaaa acaccggcaa accgcatatc cccctttttt ccgtcaaaat gcccgacttc
     6541 cgccattttc acgcaaacgc ccgattaagc caagcaattg caaagatttt ttgctagaat
     6601 agcctgcttc ttttatcaac cttttcagac ggccccacta ctttcccgcc caggaaggca
     6661 aaacggattc ggcacgaatc cggttagtat ccgtgtccga ttccaatgcc gtctgaaact
     6721 ttccggagta agaaaatgtc ccaaaaattg atcttggttt tgaactgcgg cagctcgtcc
     6781 ctcaaaggcg cggtcctgga taacggcagc ggcgaagtcc tgctcagctg ccttgccgaa
     6841 aaactcaacc tgcccgatgc ctacatcaca ttcaaagtaa acggcgaaaa acacaaagtc
     6901 gatctgtccg cacatcccga ccacaccggc gcggtcgaag ccctgatgga agaactcaaa
     6961 gcccacggcc tcgacagccg catcggcgcc atcggccacc gcgtcgtcag cggcggcgaa
     7021 ctgtacagcg aatccatcct cgttgacgac gaagtcattg ccggcatcga aaaatgcatc
     7081 ccgctcgccc ccctgcacaa ccccgcccac ctcttgggcc tgcgtgccgc gcaaagcatt
     7141 ttcaaaggcc tgcccaacgt cgtcgtattc gatacctcct tccaccaaac catgcccgaa
     7201 gtcgcctaca aatacgccgt tccgcaggag ttgtatgaaa aatacggcct gcgccgttac
     7261 ggcgcgcacg gtaccagcta ccgcttcgtc gccgacgaaa ccgcgcgctt cctcggcaaa
     7321 gacaaaaaag acctgcgtat ggtcattgcc cacttgggca acggcgcgtc catcaccgcc
     7381 gtcgccaacg gcgaatcgcg cgacaccagt atgggcctga ccccgctgga agggctggta
     7441 atgggtacgc gcagcggcga catcgatcct tccgtattcg gcttcctcgc cgaaaacgcc
     7501 aatatgacca tcgcccaaat cactgaaatg ctgaataaaa aatccggtct gctcggcatt
     7561 tccggcctgt ccaacgactg ccgcaccatt gaagaagaag ccgccaaggg gcataaaggc
     7621 gcgaaattgg ccttggatat gtttatctac cgccttgcca aatacatcgg cagtatggcg
     7681 gttgccgcag gcggtttgga cgcactggtc tttaccggcg gcatcggcga aaactccgac
     7741 atcatccgcg aacgcgtgat cggctacttg ggcttcctcg gtctgaacat cgaccaagaa
     7801 gccaacctga aggcacgctt tggcaacgcc ggcgtgatta ccactgccgg cagcaaagcc
     7861 gttgccgtgg tcattccgac caacgaggaa ttgatgattg cacacgatac cgcccgcttg
     7921 agcggtctgt aagattcaat ccccacgcga actgcctccg aaaacggagg cagttttttt
     7981 atccggcttt ccatgcttaa acagcaccgc ctcttttcag acgttgacgg ttgcagccgc
     8041 ttacctgaac cttatagtgg attaacaaaa accagtacag cgttgcctcg ccttagctca
     8101 aagagaacga ttctctaagg tgctcaagca ccaagtaaat cggttccgta ctatttgtac
     8161 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatcc gactttccat
     8221 acttaaacag cactgcctct tttcagacat tgacggttgc agccgcccac ctgaacctta
     8281 atgattgact attttttaat catgttatta ttttccataa aatacatgac attaagatgt
     8341 ttttccacaa aagatacaca caccggcaaa caccggctgt gtttatcttt tcttatgcct
     8401 attttttaat catcgtattt ttatctttta atttcaatac gcaaactaac ttatacacac
     8461 ggttttcaca tctttggact gcttccgtgt gtatagcgga tattgccgtt ttcctttctg
     8521 acaaaaatgc cgtctgagaa cttcagacgg catttgaaac atcggaatca gcggttttgt
     8581 tcataccact cgataaactt gtctgctttg acaaaaccca gcagcggctc gctgcggctg
     8641 ccgtcggagc ggacgacaaa cacgcccggc ggcccgaaca gaccgtattc tttcaacaac
     8701 gcctgatgtt cgggcgtgtt ggcggttacg tcgatttgga aaaagcgttc catatcgact
     8761 gcctgatgca cttccggctg attgagcgtg taagccgcca tttctttgca ggaaatgcac
     8821 cagtcggcat aaaaatccaa aacgacgggt ttgtcgggat gttctttcaa cgccgtatcc
     8881 atcgctgcct tcagcgcggc agtatcggca aacattttgc cgtgttccga agatttgcct
     8941 gcttcggccg gcggattgag ggtcaggaaa tggtgcagcg cggtcgtttt gccgtttgcg
     9001 ccctgccagc cgaaccacgc gccgcctatc agcaatatgc tgcccaatgc gaatgccaca
     9061 gctttcggac ggcgtttctg cctgcgtccg ttgaccagca gcatacaggc aggaaccagc
     9121 atcagcagcg tgtacagcgc gacgacgaga taatagggca agtgcggcgt ggcgaggtaa
     9181 acggcgacgg caagcaggat aaagccgaaa gcgtatttga cggcattcat ccaatcgcct
     9241 gccttaggca ggatatgccc gccgaacgtg ccgatggcaa tcagcggaac gccggtgccc
     9301 aacgccaaag tgtaaagtgc caaaccgcct aaaaccgcat cgcccgtctg accgatgtag
     9361 cccaaagcaa atgccagcgg cggggcgacg cacggcccga caatcagcgc ggacaatatg
     9421 cccataataa agacggaaac gattttaccg cctgaaagcc tgctgctttg attctgaaaa
     9481 tacgactgca cggcgttggg aagctggatg ttgaacagcc cgaacataga cagtgccaag
     9541 acgaccatca aagccgatgc cgccaacacg acccaagcct gctgcaacca tacggtcagc
     9601 agtgcgcccg tcagtccggc aacaatgccg accagcgtat aagtcagagc caaaccctga
     9661 acataaacga cggacagcac aaacgcccgc gccttgcccg cctttttgtc gccgaccaca
     9721 atactggaaa caatcggcaa caggggatac atacaggcgg taaaactcag acccaaaccg
     9781 gcgagaaaaa acgccaaaag attggcgttg agcgtatccc aagacagctt gaaacggctg
     9841 tcgccgccct catccccctt cgggggcggc agcgccccgc tgccgttttg agaggaaggc
     9901 tgcaaaaagc ggtctttggc ggatgccggt tcgtcggttt gcggatggta agtgccgttg
     9961 ccggaaatat caaactcggt atccacgggc ggatagcaca cgccggcttc ggcacagccc
    10021 tgataggtca aaaccaattt atacggttcg ccgacagcct ttgcataagg aaaggcaacc
    10081 tgcgcttcgt gatggtaaac cgtctgcctg ccgaaaaact cgtcttcctt ctcttcgccc
    10141 ttgctgaaag aaggctgtcc caacaaatcc gccggatcgg tcttgccgac gattttcgcc
    10201 tgatacatat agtatccgtc ggcaatcttg aaacggacgt tcacaccgtc gtcggcaacg
    10261 gcaagctccg gcacgaatgc cttttccggc ggcagcagat cgttcgcatc cagcgcgaaa
    10321 gctcgtccgc acaacatcaa aaatacggcg aacaggcaaa tcagtttttt cataatcgaa
    10381 tccgtttcag acaaataatt tgtctgcatt ataaatggta aggttgacgg tgggatttaa
    10441 tttatgtaaa acccgccatt atccgaacct atttccataa ccatcttatc gaacccgcca
    10501 tgtacgatgt caatacccac gatgtccgcc gctttttcgc ccgcgtgtgg cagcagcggc
    10561 tcaatccgct gcaactgagc gcactggaac agaaagccct ccgcattgtc gaagcccatc
    10621 ccgaatacca ccgctatctc gaacgcatcg aagaccatct ggacaccgac tggctgcccg
    10681 aaaacggcga aagcaacccc ttcctgcata tgtcgctgca tctgtccgtc caagaacagg
    10741 cgggcataga ccagccgcac ggcatacgcg caatccacga caccctgtgc gccaaacgcg
    10801 gctggctgga agccgaacac gaaatgatgg aggcactggc ggaaacactg tggacggcgc
    10861 aacgctacgg caccggtttg gatgtcaatt tctacatgac ccgactgcgc aaactcatcg
    10921 gcttgggtgc agaagatcaa gccaggttga acccacatga aatcgcctga ccataccaac
    10981 cgcctgcaaa atgccgtctg aagcggaaca acccctttca gacggcatgc atttccccca
    11041 tcatttccac aacgcgcgtt tcagcataat caaccaatcc ttcttatcca aaacggggcg
    11101 ttgtgcaaac acatcgtatc ggcacgcgtc cagtttctgc aaaatcaact gcgcccccaa
    11161 cacaatcata cggagttcca aaccgatacg ccctttcaat tcgcgcgcta aaggcgaacc
    11221 cgccttcagc atacgaaatg cgcgccggca ctcatacgcc atcagccgct gaaacgccgc
    11281 atccgcccta ccctccgcaa tctgtccttc ggaaacaccg aatttcaaca aatcgtcctg
    11341 cggaatataa accctgccct tttgccaatc cacagccaca tcctgccaaa aattcaccag
    11401 ttgcaaagcc gtacagatgc cgtcgctttg cgccacgcac accgcatccg ttttcccgta
    11461 caaagccagc ataatgcgtc cgacaggatt ggcagaacgt cggcaataat cggccagctc
    11521 gccgaaattt ccataccttg ttttaaccac atcctgagaa aatgcagaaa gcaaatcata
    11581 aaacggctgc aaatccaaac cgaacggcac aaccgcctcg gcatccaatc gtgcaatcaa
    11641 aggatgcgcc gaccggccgc ccgatgccaa cacgtccaac tcgcgccgca aaccctccaa
    11701 ccccgccaac ctggcttcag acggcatact gccctcgtcc gccatatcgt ccgccgtccg
    11761 tgcaaacgca tacaccgcgt gaaccggctt cctcaaacgg cgcggcaaaa tcagcgaacc
    11821 gacgggaaaa ttctcataat gcccaaccga cataccttct ccatccatca aacaaaatgc
    11881 cgtctgaaac ggaacaaacc cttttcagac ggcatcagat acctccaagc tgccggcaat
    11941 cagtggtggt aatgaccgtg cgggccgtgg acatgaccgt gtgcgatttc ctcatcggat
    12001 gcatcgcgca cgctttcaac cgtagcctta aagcggattt tcatgcctgc caaaggatgg
    12061 ttgccgtcca ccaccgcctt gccgtcggca acatcggtta cacgatagac gacaacatcg
    12121 ccggtttcag gatcgtcggc ttcaaacatc atgccgactt cgacttcaac agggaacacg
    12181 cccgcatctt cgatacggac caactccgga tcctgctcgc cgaacgcatc gtcgggcgac
    12241 agcgccacat cgaccgtatc gccggcatcc ttaccgtgca acgcctcttc caccaaaggg
    12301 aaaatgccgt cgtaaccgcc gtgcagatac gcaatcggtt cttcggtttt gtccaaaagc
    12361 tgattgttgg catcatacat ctcataatgc agcgaaacca cggaattttt cacgatagcc
    12421 atatttgtcc tttcaggaac agcagattga ttacaggcgc attctaacac aaccgccgcg
    12481 ccggccgatt accgttaacc tgttcataaa ctgtacagca catatttcaa tgtaaatctt
    12541 tgttatttta ttgcggtgta acttttttac aacattctta aaaccattcc gacctgtctg
    12601 ccgactttcc caatccgcct taataaatca tacaagatac tgaaattata ttaatctcta
    12661 taatatttat ccctatcgaa tttttaacag caaaaccgtt ttacaggatt tatcaatccg
    12721 cccgccagaa aacttttcat tcaaaccttt ttcccatctg tacgacattg caatccctta
    12781 ttccatagtg cataattacg caaattcagc gatgaatttc caacctggtt tgtagtatgg
    12841 tcgataaaga cctatttgtt tcaataattt aaattggttc taaaggttac taaaatgaaa
    12901 aaatccctgt ttgccgctgc tttgttgtct ttggttctgg cagcctgcgg cggtgaaaaa
    12961 gccgctgaag ctcccgctgc tgaagcacct gccgccgaag ctcctgctac tgaagcacct
    13021 gccgccgaag ctcccgctgc tgaagctgcc gctaccgaag cacctgccgc tgaagctcct
    13081 gctgccgaag ctgcaaaata agcattttcc gcttgcaaaa aagcaggata cgttcagtat
    13141 cctgcttttt tgatttttca gacggcatca gattcccttc ctcaatcttc tccctaccct
    13201 tccgacaaac atgcctgacc ttcataccga attttcccga ctcctgccgg cagatgaaat
    13261 tgccgaactt tctccgacgc ttttaaaaga ccagcgcaac cgctttacgt ctgcaccaga
    13321 catcattttg cagccgcgca gcgttgaaag cgtgcaaacc attatgcgtt tttgttacaa
    13381 acaccgtatt ccggttacgc cgcaaggcgg caataccggt ttgtgcggcg cggcagtatc
    13441 ggaaaacggc gtattgctga acctttccaa actcaaccgc atccgcagca tcaatttgtc
    13501 agacaactgc ataaccgtcg aagcaggttc cgtactccaa accgtccaac aggcagccga
    13561 agcctcaaac aggctgttcc cactcagtct cgccagcgaa ggctcgtgcc aaatcggcgg
    13621 caacatcgcc tgcaatgccg gaggtttgaa cgtattgcgt tacggcacga tgcgcgacct
    13681 ggttatcggt ttggaagtcg tcctccccaa cggcgaactg gtttcccatc tccatcccct
    13741 gcataaaaac accaccggct acgacctgcg ccatctgttt atcggcagcg aaggcacact
    13801 gggcatcatc actgccgcca cactcaagct ttttgcccgt ccctcggaca aggcaacggc
    13861 atgggtcggc atacccgaca tcgaatccgc cgtccgcctg ctgaccgaaa cccaagctca
    13921 ctttgccgaa cgcctatgca gttttgagct gatcggccgt tttgccgccg agttgtcttc
    13981 cgaattcagc aaattccccc tgccgacaca ttcagaatgg catattttac ttgagttgac
    14041 cgactcatta cccgacagca atcttgatga tcggcttgtc gaatttcttt ataaaaaagg
    14101 atttaccgac agcgtattgg cacaaagcga acaagaacgt atccatatgt gggcattgcg
    14161 cgaaaacatc tccgcctccc aacgcaaact gggcaccagc atcaaacacg atattgccat
    14221 tcctatcgga cgcgttgccg actttgtccg ccggtgcgcc aaagatttgg aacagaattt
    14281 taaaggcata caaatcgtct gcttcggaca tcttggcgac ggcagcctgc actacaatac
    14341 tttcctgccc gaaatcctca gcaatgaagt ctatcgttac gaaaacgaca tcaacgacgc
    14401 agtttatcgc aacgttcttg cctgcaacgg cacgattgcc gccgaacacg gcataggtat
    14461 catcaaaaaa cagtggctgg acaaagtacg cacgcctgcc gaaatcgccc tgatgaaaag
    14521 catcaaacaa caccttgatc catataacat tatgaatccg ggcaaactgc ttccgtaacc
    14581 ggcatttctg atttgcatac acaacaaaga aagggacaat agatccgatt gtcggtttag
    14641 cgcgagctcg tgagtgcggt aaaaaattgg tggaaatgac acgaaaaatg accgcatttt
    14701 taaaataaaa aaatcggcag tgaatttctc tgccgatttt attttgttac aacttaactt
    14761 aaaacgtcca ctgtaaattc aatgcgcctt gtttagcttg atgatgtttg cctgtttggc
    14821 gattgaatgt ggcttgtaaa gttaagtgag atttgatttt cactgctaca cctaattggc
    14881 tttcaattgc cgtcttattg tttatcactc gacgttctcc gtccatttct accccgaaag
    14941 gtttttgttg gtagattgaa ttgacagcaa caaatggttg gaaagtgacg ccattagtga
    15001 aagcaaattg cgctttagct tgaacgccaa cccgagtttg taattggcgg gagccaagta
    15061 aattcacgtg ggcattttcg ctatcgctga attttccgtt tacccccaaa taagtcaatt
    15121 gcgcctgtgg ttgtaggtaa aaacgaacac ggttgccctt tttagtgaag tgttccgcca
    15181 ataaagcatt gtaacccgct tcaattgagg cggtaatacc ttttgaagta aaacgttctg
    15241 tagcatcttc agtgttaatg cggtggcgga agcgttgata ttgtacccag ctatccgcat
    15301 acgcacctgt ctgtttgtcc tgaagctgat gccaagtggc gtaaacgcct gcaccaaagc
    15361 ctttcacatt tcccgttgta agattgtctg tatctgggtt gcggaaagta ctgcgttgtt
    15421 ctgcttgtcc gcccattaag ccaacggaaa gttgatagtt gtgattttgc aatgagaaaa
    15481 catcgccacc aagttgtatg cctttacgat ttccttctac tggtgcggtt ttaccttgta
    15541 catcttggct taagtgacca ctaattaaac gcaaccacaa gcctttgcgt ggtaaagtgc
    15601 ggtcaaaaat atcgctgttt ttgtcgttca aacgtaggtt gaataaagta ttggcagctt
    15661 gagcctgttg tgcataaatc gcaatatcat cgcgttcttg cactttggta aaaaagccct
    15721 ctgggcgttg ttgtaaagag agtgtataaa ttcccttttg gtgtttgcca gaaagacgga
    15781 atgcgcgttt atctgctgtg ccatttactt tgataatttg atgcccgtcg aggcttttta
    15841 aatcgtctat tggattttcg aagatgatgt tggaagtgcc agtaacattt ttctcaaaaa
    15901 ttaatgcagt atttttcgct tctttaggat cgtaagcaaa acgaaaacga gctccgccag
    15961 cataatcttc tttgacgagt aaactttcac ttttagtatt aaaacggatg tctgcattcg
    16021 ttgtttttaa ttccccaaca tcagaatccc aacggggttc ccagagagaa ttttctaata
    16081 tgaattgatc caaactaatt gtttgtccga taacgtgtga attgtctgta accgtaatat
    16141 agtggaatgg atctaaacca gaatatagat gctcttccca accagcaagc ttattaagtg
    16201 ttgggcgaag ccattcgata taattaccta cagataaaat ttcgctgttg atatggctat
    16261 tttttatttg tggagctaag gcgaaaatat cgctctttat taatcttggg ccagagtttc
    16321 cgaaggatgg ttgttcacac tgatcattat tggaattccc agtatttaca cagtccttaa
    16381 aatattgctg gagtgttttt tcttcttccc catttctttt ttcaaccccg aaacctcggg
    16441 cgaagccaac aaggtaatct tcagtgtatt ccttgtcata aaaagaaatc ttttttgagt
    16501 tgttgatgtt ttcgaattgg tgtgttttag agtatagtgc gggaactttg agagagttct
    16561 gagagctacc attcgtcggt tttagaagat aagtttcttg tttccagtat tcgctgcttt
    16621 tatctctaag tttttttaag cgatcaattt gctcatttgt taatttagaa ttgtcgtaaa
    16681 cgtaatcaac agccaaaagc ggagaggtat aaagaataga aaaaaataga cttataataa
    16741 atgatttttt aaacttctgc ttgcttgctt gcttcgagtt tcataataaa ttttcctttg
    16801 tcaagtaaaa ataaatgggg cgtggatttt agcatagaat tgaacaaaaa atgtcattta
    16861 gctcacattt ttctctattt atttcttatt tattaaaagt aaacgtttgc tttttgctat
    16921 tttgtcaagc cagtttgaaa atgtgtataa ttgccctcgt tatttacaaa aatttcagga
    16981 aaaatgaccg cactttaccc ttggctaatg ccaatttatc atcaaattgc tcaaaccttt
    17041 gacgaaggtt tggggcatca tgccgtactg attaaagcgg atgctggttt aggtgtagaa
    17101 cgtttacaca tcaggcggca gccttgccca taccgtctga agcactgttt ccacaatcag
    17161 cgcgtatgct taatcaaccg ctgtttctcg cgtttccaat ccgcctcttt catactctga
    17221 cgtttgtcgt gctgtttctt accttttgcc aaaccgattt ccatcttgat ttttccgcgc
    17281 gaaaaatgca aatccagcgg cacgatggtg tagccggcac gttcggtttt gccgattaat
    17341 ttgttgattt ccgactggtt caacaagagc ttgcgcggac gtacggcatc tggtttaatg
    17401 tgtgtcgagg ctgtgggcaa agccgtaata tggcagccga ccagataaaa cgcgtctttt
    17461 ttccaataga tataactctc tttaagctgt acgcgcgcgg cgcggattgc tttgacttcc
    17521 cagccttcca agaccaaacc ggcttcaatc cggtcttcaa tgaaaaaatc gtgaaatgct
    17581 tttttattgt tcgcaatagc cataaacatc ctatcaatat ccgccgtcag acggcataaa
    17641 cccgaaaaca gaacccatca taccgcctct tcaaccgcct gcacaatctt ctcgggatac
    17701 agcctgttga ggcagtcggt atgccccaac ggacattcac gtttgaaaca gggcgaacat
    17761 tccaagtgta ggctgacgat tttcgccctg tcgctcaaag gcggcgtatg cgtcgggctg
    17821 gaagaaccgt aaaccgccac caccttcctg cccaatgccg ccgccaagtg catcagcccg
    17881 ctgtcgttgc acacgaccgt gtccgccaac gacagcaaat ccattgcctg cgacaaatcg
    17941 gttttgccgc acaaattgac acacataccg tctgaaaggc agttgatttc ctcggcaatt
    18001 tcatcatctt tttgcgaacc gaacagccaa acctgccaac ccgccgccga ataatgtttg
    18061 cccaactcgg caaaatacct tgtcggccaa cgctttgccg gcccgaattc cgcacccgga
    18121 caaaaagcca gaacaggctt tccaatatcc aagccaaagg tttcgacaga aatttcccgc
    18181 cgccgttcat caatggaaaa ctcggggaat cccgaatgcc cgtcaaaatc ctcctggctc
    18241 ggatgcgcga gagccgtata tctgtccacc atcaaaggca gacgttcctt atccagcctg
    18301 cgtatatcgt tcaacagaaa ataacggctt tcaccgacat aacccgtcct tttaccgata
    18361 cctgtcgcca gcgcgatgat tgccgatttc aaagaaccgg gcaacacgat aacctgatcg
    18421 tatccgcgcc gccccaaatc cctaccgacc cgccaacggc gtttcaactc caacgcaccg
    18481 tgtccgaacg gattttcaag aatctcattc acttccggca tacgctcaaa caccgccatc
    18541 gaccacttcg gtgcgaacac atcaatcgtg caaccggggt gaagttcctt caaacggcgg
    18601 aacaagggct gggtcatcac gcagtcgcct atccaactgg gggaaataat caggattttg
    18661 atggacataa caagaaaccg aaatcagaca ggcggaattt taccgcgaaa ccgttggaaa
    18721 acctatcttg tctcattccg aacgccgtac gtgcaaatat gaaaaagccc gaacattcaa
    18781 gttcgggctt caaaattctg gctccccgac ctgggctcga accagggacc tgcggattaa
    18841 cagtccgtcg ctctaccgac tgagctatcg gggaatgggg cgtattatag cgtccggaaa
    18901 aaatgtgtca atccttaatt ttggaaaaat gggcgacaaa acgacaagca tatgaatcag
    18961 aaagacatta agaccgatgc cttaaaagga ttgccgttgt atgaatttcc acagccgtca
    19021 tcacaccata tttaagcccg atgagccgtt ctgccctccc ccgcttaaaa caatgccgtc
    19081 tgaacttcgc cgcgttccaa agccagtaaa aactgtttgc ggttcaaccc gcccgcgtag
    19141 ccggtcagtt tgccgtcgct gccgatgacg cggtggcagg gaatcaggat agatactttg
    19201 ttctgcccgt tggcggcggc aacggcgcgg acggctttgg ggttgcccaa acgctgcgcc
    19261 tgctccttgt agctgcgcgt ttcgccgtaa ggaatcgcca agagcgcgtc ccatgcctgc
    19321 ttttgaaact cggtgccaat ctgctccaaa ggcgtggcaa aggttttcag acgacccttg
    19381 aagtataagt ccaattcctg ccgcaaaagt tgcgtccgct catcctcccg aaacacaaac
    19441 cgtccgcgca aggctttttg gacggcggca atttcctgtt ccaaatgctt ctgtccgaca
    19501 aactccagca gacacaatcc cttatttccg aacaccgcca gcatctcgcc caaaggcgtg
    19561 gcaatggcgg cacacaccag ctcgttcaag ctgtcgggat aacgcacttc caacagacgg
    19621 atggctcggc ggatacggac atattcttca ggcgcgcagc cgatattgtc ccaaaaatcc
    19681 cgatcgaact gcccggcttc gcattccgtc agattgggat gcggcataac gccgcactca
    19741 aaaacccggg tttcgaggca atggcggatt tcatcccatt ttgacggcag attgtttaag
    19801 gaaggcaggg taatcattcg tttgctccgt atctctatca tggatttggc ggcaaaatcc
    19861 ccaattctcg tcattcccgc acgccgatgc aggaacgggc tatgacgtaa atcttgaggg
    19921 tcgggttgcg gcaatgccta aatatgcgat atttccaaag cctcagggaa aggaattaaa
    19981 gacttcagac gacctgcaaa ggctcgtctg aaaccacgat ttttgcattt gcgcattctg
    20041 gcacatcatc caaccgtttc ggcacattcc tgccgccgtt gacagcctat aatgaatcca
    20101 cttattcatc aagcaaagga atcatctatg caaacccgca tcctctccgc cgtactgctg
    20161 gctttttcaa ccgctgcctt tgccgggggc gcattcacgc tgcaattcga caacccgtcc
    20221 gaagacggcg gcttcacgca aaaccagctt ttgagcgcgc cttacggatt tggctgttcg
    20281 ggcggcaatg cttcgcccgc gctgtcgtgg aaaaatccgc ccgccgggac aaaaagtttc
    20341 gtcctgaccg tttacgataa agacgcgccg accggactgg gctggatgca ccgggtggtc
    20401 gccgacattc ccgccgatgt ccaccgccgc aacgcgacct cgctgcaatt aagccgctgc
    20461 gccaacatcg ccgaccggac tgggctggat gcactgggtg gtcgccgaca ttcccgccga
    20521 tgtccgccgc cgcaacgcgg cctcgctgca attaagccgc tgcgccaaca tcgccgacga
    20581 ccagtccgca gccatatcgg cggtaatcag tttgcggatt tgccgcatca ggttgacgcc
    20641 ttcgtacacg gcaaaaccga tgccgtcatg ctgcaaccac gccaacacgc cgcaaagcgc
    20701 ggcctccgcc gcattgtgcg gcacttcttc atccgtcagc accgccgccg cataatcaaa
    20761 cgccgcgccc atccgcccgg ggtgcggcag cttcaccgca tcgcacactg cctgcgccgt
    20821 cccgtattgt gcggcgaacc tttctacggt ttcctgttca aaagcaatcc attgcgcctg
    20881 atagaggccg tctgaatcgg gaatattaat aacgtcaaac gtctgtccgc ctgccaacgc
    20941 aaccgcctca cccgccgcaa tcctcaattc gcgcctcgcc caccgcagct tcttgcttcc
    21001 gcgccgcacg ataagcacag ctggttcata taccgccacg ctgcggtaca aggcggtatg
    21061 atgttgcacg atgccgccca aagcacccaa tcgttcgcgc gtatgaaagt aatttgacgg
    21121 cactattatc tccaacacgc agggccgcac ataaggcgca gccctgttta ccctgatttg
    21181 gaaggggtta cttcccaaaa ataaaccaaa atcccactgc cccgaatggc cgggtttcaa
    21241 ccatattccg tcggtgtgcc tgcaacttga ctccctcccc tgtgggggag ggttggggag
    21301 agggtaaaac ggggcagata cagacaatat ttccgttacc gccccgatgc cctctcccta
    21361 tccctctccc acgaaagagg gaatggattg ccgtttaaat aaaaaaatct acataaaaat
    21421 caatgtttta tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
    21481 aaatagtacg gcaaggcgag gcaacgccgt actggtttga atttaatcca ctatatctca
    21541 aacccacatt aggtctaatc aaatggtcgg acatccatat ccgacagccc attttctttt
    21601 cagacggcat ttccagccaa caagcgcgcc aatatcccct catacaccgc cgacagcttc
    21661 ggaatatcgt ccagccgcac gttttcgttg atttggtgga tggtcgcatt ggacgagcct
    21721 aattcgatga gttcttttgc aatggctttg atgaagcgtc cgtccgaagt gccgccggtg
    21781 gtggacaatt cggtctctac gccgcagatt tcggcaatgg cggcgcgtgc cacgtcggtc
    21841 agtttgcccg cgtgggtcag gaagggctgc cccgaacacg accactgcaa atcgtattgc
    21901 acaccgtgtt tgtccaaaat ggcgtggacg cgttgtttca gccctgcttc ggtggactcg
    21961 gtggagaaac ggaaattgaa tttgacattc agctcgcccg gaatgacgtt ggtcgcgcct
    22021 gtgccgccgt tgatattgga aatctggaaa ctggtcggcg ggaagtattc gttgccttcg
    22081 tcccagactt cctgcgtcag ctctaacaag gccggggcaa aagtatggac gggattgatt
    22141 gccaaatgcg gataggcaat atgaccttgc ttgcctttga cggtcaggtt gcccgacagc
    22201 gagccacgcc ggccgttttt aatcatatcg cccaatttgc ccacggcggt cggctcgccg
    22261 acgatgcagt agtcaatcaa ctcgccgcgc gctttcaaca catcgacgac tttggtcgta
    22321 ccgtccagcg cgtcgccctc ttcgtcggaa gtaatcagga gcgcgatgct gccttggtgg
    22381 tcgggatgtt cggcaacaaa gcgttcgcag gcggtaacaa aacaggcgat gctggttttc
    22441 atatccgccg caccgcgccc gtataatctt ccgtcgcgct cggtcggctc gaacgggggc
    22501 gaatcccatt tttcaacagg gcctgtcggc acaacgtcgg tatgccctgc aaaacagacg
    22561 acgggaactt tcgtgccgcg tcgcaaccag atgtttttgg tgtcgccgaa atggagttct
    22621 tcagccgcaa aaccgatttt gtgcaggcgt tcggccagca gttgttggca gttttggtca
    22681 tctggcgtca cggatggtcg ggaaatcagc tctttggcaa gctctaggga ttgagtttcg
    22741 gtcatatttg ttcacttttg aaattagacc gtctgaaacg ttctgaatgt gattttcaga
    22801 cggcatttag gttaggttgg catacggggt gggtattttt acccgtcagt cttctgaatc
    22861 atttgccgtg gcaggcttcg taaagcggca gcaaatcttc caccgtttcc gctatccatt
    22921 tcgcgacatc ctgcctgccc aaatcgtcgc gttcgatgtg tttgccgatg cagaaaaagt
    22981 cttcgtcgtt ttgcaacttt cggtcggact cgttttgttg cgcgacggtg cggtagtcgt
    23041 cgtattcgct ttccgcaccg tgccacatat cgaaagaagc gtatttttcg gtatcaaaat
    23101 tatccaacca gcggttgtaa tcaggcagcg cgatgggcga cacgtcggct ttgtagcagt
    23161 gccaatccaa gctgacgctc aaacggcggc ggttgagcag tatcgacaaa atcgcggcgg
    23221 aatttttata ttgttcgtat ttgaagtagg caaagaaatg ggcgcgaacc tgccagccgt
    23281 tacaccagcg ttcgatgtgc ggaggcgcaa acggcgcacc caattcggcg gcaacctgct
    23341 gaatcagctg ctgccatatc tgccagtttt ctttatagtc agccttgatt tgcggaatgc
    23401 tttcaggctg gtatttttta agctgggaaa attggaaaaa cgggatattg aacaaatcgc
    23461 aacttttcgg ggtcagcata atatatcctt gagacgattg tttcagacgg cattatttgc
    23521 gccggcgcgc cgccataatt tcgccgattt cggtcagttt ttcttttggg ataaaggtgt
    23581 tgcccatatc aaacagcggc tcttcaatcg ccagatgcac atcgtaacct gccacaaaac
    23641 gtttgaacgc ttcctcatcg gggacataag cgttgtctgc ttcgagtttg gcaaattcgg
    23701 cagccacggc atcccagttg ccatacaggc tcacatgctg acgcaaaagc tcgtccacgc
    23761 tttcttgggc ttgcggcgcg tattgcagca gcagcggaaa gaagttttct tcttcgtctt
    23821 catggtgcag cggcgcggca acgttgaaat accgggcgat ttggcggatg gtttgcaaaa
    23881 caatctgatt gcagccgttt tcggcgatat agtccgacag catggcgact tgtccgcaaa
    23941 aacggcgtac tttgccatgg caggcataca gcatttcaat cggttcggca aaggtaacgc
    24001 ttttggtttc aaacggattc atggtttttt agattttatt cttaaattaa cgtttccaga
    24061 ttataacaaa acagcctgct caaggcaggc tgtctgtttt caagctttag gcgaattagt
    24121 caaccaaagt aattttaccg ttcatcaaag caccgtgacc cgggaaggtg caggcaaatt
    24181 tgtagtcgcc gtcagccaat ttggcaggat ctagagtcag ggaagactct tcgccgccgc
    24241 cgatcagttt ggtgtgggca acaacgcgcg catcgtcagg tttaacgtag tcagtgtcag
    24301 ctgcgccaac gccatctttg aaaataccgt ccatgtcttc agttttaccg atgacaatgt
    24361 tgtgacccat gctggtttta ggttgggtac cggtgtgttt cagggtgatg gtgaattcct
    24421 tacaagcttt gcttacttgg atgtctttag tgttgaactg catattgtcg ttggattcga
    24481 cagttgccgc acagttgccg gcagcagggg cttcggcagc atctgcagga gcagcttcgg
    24541 cggcaggcgc ttcggaagcg ggtgcttcag cagcaggagt tgcctcggca gcaggcgcgg
    24601 caggttcttg agagcaggca gccaaaccga taacggcggc agaaatcaga gccagatacg
    24661 ctttcataac aaatctccaa tcgataaaat aatattcggt tttacagaaa tcaaagtgca
    24721 accgccatta acaaaacctt gaaaaagatt ccgccgcgtt gcacaaacag atgtttcgga
    24781 gcggcatttt gctacaaatt tcatttgaaa tcaaagcctg tctgcaagtt tacaatcgtt
    24841 tactttaaaa ggggcaattt taccccgaac ctatttcttt agtattagac ccattatcct
    24901 ttacttctta atattaacgg atgtttacac aaattcccgt atacatttta tgcgccatgc
    24961 cttctaacca agtttgccaa tgcctccgcc aattcgggat gccgtttttc caactttacc
    25021 gccgccgaac cgaaactctc cagcgcagcc ttactcaaat gcagggtatt ggttttcggc
    25081 ggtttttccg gtttcggggc cagcctgacc gaaacagagc gtatcgaagc atcaagccct
    25141 gccaactgcg gcaataccga cggtgcaatc attttcaagc gcgatgccgc catattgttt
    25201 gccgccaaaa ggacaagcct gccgtcttcg atacatgccg tctgaaaatg cgggtgcagg
    25261 ttggcaggca gcagtttttt cacggcggca tccaaccgcc gccactgtcc cgcctgtttc
    25321 aaaagtccgg aaagcagcgc gtcccgcctg cccaactgtt ccaaattcat aaaacataca
    25381 cccaaaaaga ttgaaatacc gcaaacgcgc ctttatttca gacggcatta gcactttgca
    25441 caaacgcttg tgttaaaatc gcgttttcgc ccactattat atcaggcgca ggaattattc
    25501 atgctgacaa acattgccaa gaaaatcttc ggcagccgca acgaccgctt gctgaaacaa
    25561 taccgtaaat ccgttgccag aatcaacgcg ctcgaagaac agatgcaagc cctaagcgat
    25621 gctgatctgc aagccaaaac tgccgaattc aaacaacgcc tcgccgacgg tcagactttg
    25681 gacggcattt tgcccgaagc cttcgccgtc tgccgcgaag cgtcccgccg caccctcggt
    25741 atgcgccact tcgacgtgca gcttatcggc ggtatggtgc tgcacgacgg caaaatcgcc
    25801 gaaatgcgta ccggcgaagg caaaaccctg gtcgccaccc tcgccgtcta tctcaacgcg
    25861 ctggccggca aaggcgtgca cgtcgttacc gtcaacgact acctcgcctc gcgcgacgca
    25921 ggcattatgg agccgctcta caatttcctc ggcctgaccg tcggcgtgat tatttcagat
    25981 atgcaaccgt tcgaccgtca aaacgcctat gccgccgata tcacctacgg caccaataat
    26041 gaattcggct tcgactacct gcgcgacaat atggttacca accaatacga caaagtgcag
    26101 cgcgaattga attttgccgt tgtcgatgaa gtggattcca tcttgattga cgaagcgcgc
    26161 actccgctga ttatctccgg ccaggcagac gacaacatcc agttgtacca aatcatgaac
    26221 accgttccgc cccacctcgt ccgtcaagag acagaagaag gcgaaggcga ctattgggtc
    26281 gacgaaaagg cacatcaggt catcctgagc gaagcaggtc acgaacacgc cgagcaaatc
    26341 ctgacccaaa tgggattgct ggcagaaaac gactccctct attccgccgc caatatcgcc
    26401 ctgatgcacc accttatggc ggcattgcgc gcgcattccc tcttccacaa agaccaacat
    26461 tacgtcatcc aagacggcga aatcgtcatc gtggacgaat ttacaggtcg gctgatgtcc
    26521 ggccgccgct ggtccgaagg cctgcatcaa gccgtagaag ccaaagaagg cgtggaaatc
    26581 aaacgcgaaa accaaaccct tgcctccatc accttccaaa actatttccg cctgtacacc
    26641 aagctctccg gtatgaccgg tacggcagat accgaggctt tcgagttcca aagcatctac
    26701 aacctcgaaa ccgtcattat cccgaccaac cgccccatac agcgcaaaga cttcaacgac
    26761 cagattttcc gttccgccga agaaaaattc gaagccgtcg tcaaagacat cgaggaatgc
    26821 cacaaacgcg ggcagcccgt cctcgtcggt acaaccagca tcgaaaactc cgaactggtc
    26881 tcccaccttc tgcaaaaagc cggactgccg cacaacgtcc tcaacgccaa agaacacgaa
    26941 cgcgaagcac tgattgtcgc ccaagccggc aaagtcggcg cgattaccgt tgccaccaat
    27001 atggcgggac gcggtacgga catcgtttta ggcggcaacc tgaagcacca aaccgatgcc
    27061 atccgtgccg acgaagcctt gagcgacgaa gagaaacagg cacaaatctc cgcactcgaa
    27121 gacggctggc aggcagaaca cgacaaagtg atggaagcag gcggtttgca catcatcggt
    27181 acggaacgcc acgaaagccg ccgtatcgac aaccagttgc gcggacgttc cggccgtcag
    27241 ggcgaccccg gatccagccg tttctacctc tcgtttgaag acccgctgct gcgcctattc
    27301 gcactcgacc gcgccgccgc catcctcaac cgcctcgccc ccgaacgcgg cgttgccatc
    27361 gagcacaacc tgctgacgcg ccaaatcgaa ggggcgcaac gcaaagtcga aggcagaaac
    27421 ttcgatatgc gcaaacaggt tctggaatac gacgacgtcg ccaacgagca acgtaaagtc
    27481 atttacagcc aacgcaacga aattttaacc agcaaagaca tcagcgacct gatgaaggaa
    27541 atccgttctg atgtcgtcag cgacctcgtg gatacctata tgccgcccga cagcatggaa
    27601 gaacaatggg acatcccgac tttggagaac cgtctggctg ccgaattcag actgcacgaa
    27661 gacatccaat cctggctgaa ggcggacaat gcgattgacg gtcaagacat caaagaacgc
    27721 ctgatcgaac gcatcgaaaa cgaatatgcc gccaaaaccg aactggtcgg caagcaggca
    27781 atggccgatt tcgagcgcaa cgtgatgttg caggtcatcg acaaccaatg gcgcgaacac
    27841 ctcgccgcta tggactacct gcgacaaggc atacacctgc gcagctatgc ccaaaaaaat
    27901 ccgaagcagg aatataaacg tgaagccttt accatgttcc aagacctgtg gaacggcatc
    27961 aaattccata ttgcctccct gcttacctcg gttcaaatcg aacaaaaccc tgtcgcggtg
    28021 gttgaagagc aacccatcgg caacatccag tccatccatt ccgaatcgcc cgatatggaa
    28081 gaacttttgg gtcagtcgca aaccgatttg gttaccgaag cctttaatcc cgatgggaca
    28141 gatttcagcc ccgaagcctt ggaagcgcgg gggcaaatcg tccaccgcaa cgacccctgc
    28201 ccctgcggca gcggtttgaa atacaaacaa tgccacggca aactggctta agcgtttgaa
    28261 cgcaaatgcc gtctgaacat cccgctcccg tttcagacgg cattttgcct gaaccgccac
    28321 atccgactgc cattccgaaa aatcccgatt tcgtaccgtc cgtaccaaaa acagacatcc
    28381 cgtccgcccc acatcatgat tccatccgac ttcattgacg agcttttagc caaaaccgat
    28441 attgtcgata ttatcgacga gcaggttccg ctgaaaaaag gcggggcgaa ctatatggcg
    28501 tgttgcccgt tccacaagga aaaaacgccg tcgttttcgg tcagtccaac caagcagttt
    28561 taccattgtt tcagttgcgg ggcgcatggt tcggcgattg gttttgtgat ggagcatcag
    28621 gggctgtcgt ttccggaggc tgtgcagttt cttgccgacc gtgtgggcat ggttgtgcct
    28681 aaagtgcgtg ggcagaacga taatcccgaa gtccgtgccg aacgtaagaa aaaaaagcag
    28741 acgctggaag aaacgacggc ggcggcggct gatttttacg cgcaacagct aaaattcaat
    28801 ccggcggcaa aagcttattt ggacaagcgc ggcttgagtg cagaagttat cgcgcattat
    28861 ggtttgggct acgcgcccga cggctggcag cctttggcgc aagtgttcca accgtatccg
    28921 aataccgcgt tggtggatac ggggatggtg attgacaatg aggggcggta ttacgaccgc
    28981 ttccgccatc ggattatgtt ccctatccgc aatccgcgcg ggcaggtcat cggtttcggc
    29041 ggcagggtgc tggacgactc caaacccaaa tatctaaact ctcccgatac acctttgttt
    29101 gacaaaggga aaaaccttta cggcttgtat gaggggcgtg ccgctgtcaa ggaagcggag
    29161 cggattctgg tggtcgaagg ctatatggac gtggtcgcgc tggcacagtt cggcgtgggc
    29221 tacggtgtgg cggccttggg cacggcaaca acagctgaac acgtcaaaat cctaatgcgt
    29281 caggcagaca gtatttattt ctgtttcgac ggcgacagcg cggggcgaaa agcggcttgg
    29341 cgcgcgctgg aaaacgcgtt gccgcagttg aaagacgaca aatcgctgca ttttttgttc
    29401 ctgcccgagg aacacgaccc cgacagttac atccgtgcct acggcaaagc gcaatttgaa
    29461 gacgcgcttc tgaatcaaag caagcctttg tcggagtatt tctgggaaca cctttcagac
    29521 ggcattcatc tcaatacgca ggaaggcaag gcggaattgg tgaaaaccag ttcgccgctt
    29581 ttggcgcaga ttaccgcgcc ggcattggct tatttgttaa aacaacggct tagcgaactg
    29641 gtcggcatcg accccgacaa cctcgcgcaa ctgctaggac aggaagcgcc gaagcggcac
    29701 gtcaaacaaa aaaactacaa actgcctccg atttccgtca aacagcccgt catgctgacg
    29761 ctggtacagc ggcaaatccg cagcctcttg ataaatccgg attgggctgc atatatagac
    29821 ctgcccgatt atctggcgtt ggacggtgat ttcgcctgcc ttgccaatct tgccgaatcg
    29881 attaaaaacc atgccgccgt acccgaaacc gctcaggttt tagagtatat gcgcggctcg
    29941 ccttacgaag aaacgataac ccgaatcttc cattcaacgc accaatcgga agaaatgaac
    30001 agcagcagtg aagaagattg cgagaatttc caaatcggca tgaaaaaact gctcaatgag
    30061 ttaaaataca gccaaatcga agcattgaaa caaaaaagcc tgcaatccgg cttaaatgaa
    30121 agcgagaaaa aacttttgct gtcgttgctg accgcaaaac aaaattgacc ggcggattcc
    30181 gccacccgta aacccgttat gccgtctgaa aagcacttac cccggctgca acaacgacac
    30241 ctgcagaaca cccatcccaa aaagccttca gacggcatca gagtacccgc tctgccacgc
    30301 cttcaggtgc gttcaaacgc aaaccgtcgg caccttacca acagaaagca gacaatgtcc
    30361 agaaaccaaa atcacgaaga atatcaagac gacacccgtc cgttaagcat tgaagagcaa
    30421 cgcgcgcgcc tgcgtcagct catcatcatg ggtaaagaac gcggctacat cacctactcc
    30481 gaaatcaacg acgccctgcc agacgatatg tctgatgccg accaaataga caatatcgtc
    30541 agcatgattt ccggtttggg catccaagtt accgaacacg cccccgatgc ggaagacata
    30601 ttgttaagcg acaatgccgc cgttaccgac gatgatgccg tcgaagaagc cgaggccgcc
    30661 ctttccagtg cagattccga gttcggcaga accaccgacc ccgtccgtat gtatatgcgc
    30721 gaaatggggc aggtcgacct gctgacccgc gaagacgaaa tcatcatcgc aaaaaaaatt
    30781 gaaaacgccc tgaaaaatat ggttcaggcc atctccgcct gcccgggatc cattgcggaa
    30841 atcttagaac tcatcgaaaa aatccgcaaa gacgaaatcc gcgtcgacga agtcgtagaa
    30901 gccattatcg acccgaatga agtattgctc aacgaattgg gcttggggca cttggaaacc
    30961 acagcgcccg agaaaccttc caacgacaat tcggacgaaa acgaagacga cgaagaatcg
    31021 gaagaagatg cggatgaaat ctcggcagcc aatctcgccg aattgaaaca aaaagtcatc
    31081 ggccactttg cccaaatcga aaaagactac aaaaaaatga ttggccgttt ggaaaaacac
    31141 cacagccggc acaaagacta tctcgcctac cgcgacgcga ttgccaacaa actgctggaa
    31201 gtccgtttcg ccacccggca aatcgacagc ctcagcagca gcctgcgcgg gaaagtagaa
    31261 aacatccgca aactcgaacg cgaaatccgc gacatctgcc tcgaccgcgt ccatatggaa
    31321 cgcgactact tcatccaaaa cttcctgccc gaaatcacca atctagaatg gattgaagaa
    31381 gaaatcgcca aaggcagggt ttggagcgac gcgctcgacc gcttccgcca cgccatcctc
    31441 gaaaaacaaa ccgagttggc ggatatggaa aaagaaaccc gcatttccat cgaagagttg
    31501 aaagaaatca acaaaaatat ggtgtcaagc gaaaaagaaa ccgcagccgc caaacaggaa
    31561 atgattcagg caaacttgcg cctcgtgatt tccatcgcca aaaaatatac caaccggggc
    31621 ttacaattcc ttgatctgat tcaggaaggc aacatcggtt tgatgaaagc ggtcgataag
    31681 ttcgaatacc gcagaggcta taaattctcc acctacgcaa cctggtggat ccgccaggca
    31741 attacacgct cgattgccga tcaggcgcgt accatccgca ttccggtaca tatgattgaa
    31801 accatcaaca agatgaaccg catctcgcgc caacaccttc aagaaaccgg cgaagaaccc
    31861 gattccgcca aacttgccga actgatgcag atgcccgaag acaaaatccg caaaatcatg
    31921 aaaatcgcca aagagccgat ttcgatggaa acccccatcg gcgacgacga cgattcgcac
    31981 ttgggcgact tcatcgaaga tgccaacaat gttgcgccgg ccgatgcggc aatgtacacc
    32041 agcctgcacg aagtaaccaa agaaatcctc gaaagcctga caccgcgtga ggcaaaagtc
    32101 ctgcgtatgc gtttcggcat cgatatgaac accgaccaca cgctggaaga agtcggcaga
    32161 cagtttgacg taacgcgcga acgcatccga caaatcgagg caaaagcact ccgcaagctg
    32221 cggcatccga caagaagcga ccgtttgaga agtttcttgg acagcgaaga cagcaagctg
    32281 taaaccaaaa aaccgcaggt ttcaaatacc tgcggttttt tcttacacaa taaacaacgc
    32341 ttccacatat cccacactcc tatcccgaga cctttgcaaa at
//
LOCUS       DAWFIT010000013        31544 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFIT010000013 DAWFIT010000000
VERSION     DAWFIT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.013"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(106..1080)
                     /locus_tag="WGQ85_000580"
     CDS             complement(106..1080)
                     /locus_tag="WGQ85_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0750063.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            1177..2232
                     /gene="tal"
                     /locus_tag="WGQ85_000581"
     CDS             1177..2232
                     /gene="tal"
                     /locus_tag="WGQ85_000581"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0750064.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            2374..2664
                     /locus_tag="WGQ85_000582"
     CDS             2374..2664
                     /locus_tag="WGQ85_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750065.1"
                     /translation="MAKALEIISPNEIYSDLIFKNPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            2681..3568
                     /gene="gluQRS"
                     /locus_tag="WGQ85_000583"
     CDS             2681..3568
                     /gene="gluQRS"
                     /locus_tag="WGQ85_000583"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0750066.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPCQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(3638..4648)
                     /gene="dusA"
                     /locus_tag="WGQ85_000584"
     CDS             complement(3638..4648)
                     /gene="dusA"
                     /locus_tag="WGQ85_000584"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0750067.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(4679..5170)
                     /locus_tag="WGQ85_000585"
     CDS             complement(4679..5170)
                     /locus_tag="WGQ85_000585"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0750068.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(5244..6002)
                     /locus_tag="WGQ85_000586"
     CDS             complement(5244..6002)
                     /locus_tag="WGQ85_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0750069.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAHQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(6073..6939)
                     /locus_tag="WGQ85_000587"
     CDS             complement(6073..6939)
                     /locus_tag="WGQ85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0750070.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(6988..7266)
                     /locus_tag="WGQ85_000588"
     CDS             complement(6988..7266)
                     /locus_tag="WGQ85_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0750071.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(7266..7556)
                     /locus_tag="WGQ85_000589"
     CDS             complement(7266..7556)
                     /locus_tag="WGQ85_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0750072.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(7559..8089)
                     /locus_tag="WGQ85_000590"
     CDS             complement(7559..8089)
                     /locus_tag="WGQ85_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0750073.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            8397..10922
                     /locus_tag="WGQ85_000591"
     CDS             8397..10922
                     /locus_tag="WGQ85_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0750074.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(11095..11571)
                     /gene="gloA"
                     /locus_tag="WGQ85_000592"
     CDS             complement(11095..11571)
                     /gene="gloA"
                     /locus_tag="WGQ85_000592"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0750075.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(11535..12704)
                     /gene="lapB"
                     /locus_tag="WGQ85_000593"
     CDS             complement(11535..12704)
                     /gene="lapB"
                     /locus_tag="WGQ85_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0750076.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(12715..13029)
                     /locus_tag="WGQ85_000594"
     CDS             complement(12715..13029)
                     /locus_tag="WGQ85_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0750077.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            13339..14337
                     /gene="ilvE"
                     /locus_tag="WGQ85_000595"
     CDS             13339..14337
                     /gene="ilvE"
                     /locus_tag="WGQ85_000595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0750078.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(14581..15366)
                     /gene="fabI"
                     /locus_tag="WGQ85_000596"
     CDS             complement(14581..15366)
                     /gene="fabI"
                     /locus_tag="WGQ85_000596"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0750079.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(15521..16342)
                     /gene="dapD"
                     /locus_tag="WGQ85_000597"
     CDS             complement(15521..16342)
                     /gene="dapD"
                     /locus_tag="WGQ85_000597"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0750080.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(16352..16582)
                     /locus_tag="WGQ85_000598"
     CDS             complement(16352..16582)
                     /locus_tag="WGQ85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750081.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(16552..18195)
                     /gene="pgi"
                     /locus_tag="WGQ85_000599"
     CDS             complement(16552..18195)
                     /gene="pgi"
                     /locus_tag="WGQ85_000599"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0750082.1"
                     /translation="MDAFTRAWCALERHYQDTRHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLERLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            18705..19937
                     /gene="pilG"
                     /locus_tag="WGQ85_000600"
     CDS             18705..19937
                     /gene="pilG"
                     /locus_tag="WGQ85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0750083.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            20011..20871
                     /locus_tag="WGQ85_000601"
     CDS             20011..20871
                     /locus_tag="WGQ85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0750084.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            20873..21505
                     /locus_tag="WGQ85_000602"
     CDS             20873..21505
                     /locus_tag="WGQ85_000602"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0750085.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            21498..21707
                     /locus_tag="WGQ85_000603"
     CDS             21498..21707
                     /locus_tag="WGQ85_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0750086.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(21792..23468)
                     /gene="pilB"
                     /locus_tag="WGQ85_000604"
     CDS             complement(21792..23468)
                     /gene="pilB"
                     /locus_tag="WGQ85_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0750087.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(23609..23671)
                     /locus_tag="WGQ85_000605"
                     /pseudo
     CDS             complement(23609..23671)
                     /locus_tag="WGQ85_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23869..23940)
                     /locus_tag="WGQ85_000606"
     tRNA            complement(23869..23940)
                     /locus_tag="WGQ85_000606"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23905..23907),aa:Arg,seq:cct)
     gene            complement(23960..24406)
                     /locus_tag="WGQ85_000607"
     CDS             complement(23960..24406)
                     /locus_tag="WGQ85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0750088.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(24411..25385)
                     /gene="ispB"
                     /locus_tag="WGQ85_000608"
     CDS             complement(24411..25385)
                     /gene="ispB"
                     /locus_tag="WGQ85_000608"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0750089.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(25401..25589)
                     /locus_tag="WGQ85_000609"
     CDS             complement(25401..25589)
                     /locus_tag="WGQ85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750090.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            25609..25917
                     /gene="rplU"
                     /locus_tag="WGQ85_000610"
     CDS             25609..25917
                     /gene="rplU"
                     /locus_tag="WGQ85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0750091.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            25942..26214
                     /gene="rpmA"
                     /locus_tag="WGQ85_000611"
     CDS             25942..26214
                     /gene="rpmA"
                     /locus_tag="WGQ85_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0750092.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            26553..27737
                     /gene="ubiM"
                     /locus_tag="WGQ85_000612"
     CDS             26553..27737
                     /gene="ubiM"
                     /locus_tag="WGQ85_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0750093.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIECSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(28008..28163)
                     /gene="rpmG"
                     /locus_tag="WGQ85_000613"
     CDS             complement(28008..28163)
                     /gene="rpmG"
                     /locus_tag="WGQ85_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0750094.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(28195..28428)
                     /gene="rpmB"
                     /locus_tag="WGQ85_000614"
     CDS             complement(28195..28428)
                     /gene="rpmB"
                     /locus_tag="WGQ85_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0750095.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(28678..30204)
                     /gene="farB"
                     /locus_tag="WGQ85_000615"
     CDS             complement(28678..30204)
                     /gene="farB"
                     /locus_tag="WGQ85_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0750096.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(30228..31382)
                     /gene="farA"
                     /locus_tag="WGQ85_000616"
     CDS             complement(30228..31382)
                     /gene="farA"
                     /locus_tag="WGQ85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0750097.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         7650 a         8936 c         7902 g         7056 t
ORIGIN      
        1 cagtacagat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
       61 tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata caatccgcgc
      121 cgccaaaagg tcgtgcatat tcagcgcgcc gatcagcacg ccatctgcat cggtaaccag
      181 aagcccgttc acatggtttg cctgcatgac tttcagggct tcggtggcga gacgttcggc
      241 ggagatggtt ttagggtgtg tatgcatgac ttcgtctatc gaaagaccgg taaaattgtc
      301 gcattcttga aacaggcggc gcaaatcgcc gtcggtgaat acgcctttca gacggccttg
      361 cccgtccgtt accgccaaca tgcccagccc tttttcactc atgctgacga tggcttcttt
      421 caagggcgtg ccgagtcgga cggcaggcag gccgccgcct ttgtgcataa tgtcggcaac
      481 gcgcaaaagt aggcgtttgc cgaggctgcc ggcagggtgg ctcaaggcga agtcgtcggg
      541 cgtgaacgcg cgggcgcgca gcaggacaac cgccaacgca tcgcccaaag ccataacggc
      601 ggtggtgctg gtggtcgggg caagccccag cgggcaggct tctttggaaa ccgacgccgt
      661 gatgtggatg tcggcatggc gcgccatggt tgaatcgggg cgggcggtga tgcagacaag
      721 cgtgatatct ttgcgtttga gcgcggggat gatggcggcg atttcgtcgc tttcaccgga
      781 attggaaatc gcgacgacca cgtcgttgtc cacaatcatg cccaaatcgc cgtgtgccgc
      841 ttccgcaggg tggacgaaaa acgcgggcgt gccggtcgag gccatggttg ccgccatttt
      901 gcgcccgata tgtcccgact tgcccatgcc cgtgataacg accctgcctt tgcagtgcaa
      961 caacgcgtcc gccgcaaggg cgaagttttc gtccaaatcc gccgcaattt cgcgcaagcc
     1021 ttccgcttcg gtgtgcaaca cttcgcgtgc ccaatcaaga tatttttcgt ttcctgccat
     1081 cgccatccgc ctttgctgta ataaaactac ccaaaatttc cgaatcgggg tagcataagc
     1141 ctttgaaaca tacacatcca tccaaaggaa tcagaaatga ctattttatc ggacgttaaa
     1201 gcattaggac aacaaatctg gctggacaac ctttcccgct cgctcgtgca aagcggcgaa
     1261 ttggcgcaaa tgttgaaaca aggcgtgtgc ggcgtaactt ccaatcccgc catctttcaa
     1321 aaagccttcg ccggcgatgc gctttacgcc gacgaggttg ccgccctcaa gcagcaagac
     1381 ctgaccccca aacaacgcta cgaaacgatg gcggttgccg atgtgcgtgc cgcctgcggc
     1441 gtctgtcttg ccgaacacga atccaccggc ggcaaaaccg gcttcgtcag cctcgaagtt
     1501 tcgcccgaat tgtccaaaga cgcgcaaggc acggttgaag aagcgcgccg cctctacgcc
     1561 gccatcgggt gcaaaaacgc gatgattaaa gtgcctgcca ccgacgcagg catcgatgcg
     1621 ctcgaaaccc tcgtttcgga cggcatcagc gtgaacctga ccctgctgtt ctcacgcgcc
     1681 caaaccctca aagcctacgc cgcctacgcg cgcggcattg ccaaacgctt ggcagccgga
     1741 caaagcgttg cccatatcca cgtcgtcgcc agcttcttca tctcgcgtgt ggacagtgcg
     1801 ctggacacaa cactgcccga ccggctcaaa ggcaaaatcg ccatcgccct tgccaaagcc
     1861 gcctatcaag attgggaaca atatttcacc gcccccgaat ttgccgcact ggaagcccaa
     1921 ggcgcaaacc gcgtgcagct tttatgggca tctaccggcg tgaaaaaccc agcctatccc
     1981 gacacgctct acgttgacag cctgatcggc gcgcacaccg tcaacaccgt tcccgatgcc
     2041 acgctcaaag cctttatcga ccacggcaca gccaaagcca cgctgaccga aggcgtagaa
     2101 gaagcacaag cgcagctcgc cgaaaccgcc gcgctcggca tcgatgtcga aaccttggca
     2161 acgcgtttgc aggaagacgg tttgaaacag tttgaagaag cgtttgaaaa actgctcgca
     2221 ccgttggttt aaacttttca tccccgatgc cgtctgaaag gctttcagac ggcatcgccc
     2281 ttttgaccgg acacagtggg ttcaccctaa aaatattaat caacaagaag ttaatgcacg
     2341 acattaccca ccaacacaaa aggaaataga atcatggcaa aagcactcga aatcatttca
     2401 cccaatgaaa tttattcaga tctaattttt aagaatccgg tacctcccca tactatatat
     2461 acagaattga tgaaactgat tggtagaaat gcaagtaatg agagaatcgc agatagagcc
     2521 ttcgattttt tctcgtccgg ccttatggat ggttcagcta ccgaagagca atataatgct
     2581 ctatatgatt taaccttgct tgaagagccc gggatggaac tgaataaaga tgagataatg
     2641 gctttgattc atagtcttaa ataaaggggc tgtaccggat atgtacacag gacgtttcgc
     2701 ccccagcccg accgggctgc tccacatcgg ctcgctgctg accgccgccg cttcctatgc
     2761 cgatgcacgc tcaaacggcg gcaaatggct ggtccgcatg gaagacctcg atccgccgcg
     2821 cgaaatgccg ggggcggcaa gccatatcct gcacacgctt gaggcattcg gattcgagtg
     2881 ggacggagaa gtcgcctatc agagccaccg ttacgccctg tatgaagaaa ccctatgccg
     2941 tctgcaaacc gccggactgg tctatccctg ccattgcagc cgcaaagact ggcaggccgg
     3001 ggcaaggcgg ggcgcagacg gattcgtcta taacggacgt tgccgccacc cctgccaacg
     3061 ccctgcaccg caaggcaaac agccggcgtg gcgcatccgc gtccccgacc gcattatcgg
     3121 tttttcagac ggcatcgtcg gcggttacgc ccaaaacctc gcccgcgaca tcggcgattt
     3181 cgtcctgctt cgtgcagacg gttactgggc ataccaactc gccgtcgttg ccgacgatgc
     3241 cgaacagggc gttacccaca tcgtccgcgg acaagacctg cttgtttcta cgccgcgcca
     3301 aatctatttg cagcagtgtt tggacgttcc gacaccgcaa tatgcccacc tgccgctgct
     3361 gaccaacgca caagggcaga aatggtcgaa acagacgctc gcacccgcat tggatttaaa
     3421 ccgccgcgaa caactcctcc gccaagtgtt ccgttacctc aacctgcccg aagcaccgga
     3481 agccgaccgc cctgccgaac tgctcgactg ggcggtggca cactgggata tgggcaaagt
     3541 gccgaaacac gccattaccg ccccctaaac accgcccgac aaacgcacaa atgccgtctg
     3601 aaaccttcag acggcatttc atacacacag ccccgcccta ttcgcgcata tttgcccgtt
     3661 cgacttcttt ccacgcttcg agaatcaggc cgccgtcgtt gtctttcaag agcgttgcat
     3721 cggaaagcat acgccgccaa gtgcgggcat ttttcagacc gtgcatcagc ccgaggctgt
     3781 ggcggacgat gtggcgcaag attgtgccgc gtccggcttg gatttgggct tgactgtatg
     3841 tgtagagacg ctgcaccaaa tcggcgtatt caatcgggct gcgggtatcg ccgtaaaaca
     3901 gcctgtccca ttcgcgcatc accatcgggt tgtggtacgc ctcgcgcccg accatcacgc
     3961 cgtcaacgtg ttgcaggtgt tgcgcgattt cctcattggt cgtgatgccg ccgttgatga
     4021 tgatttccag cccgggaaac tcttgcttga ggcggtaaac ataatcgtat ttcaagggcg
     4081 gaacgtcgcg gttttctttg ggtgaaagac cgtccagcca cgcgttacgt gcgtgaacga
     4141 taaaggtttt gcaggcggtt ttgtcgcgca gcgtgccgac gaaatcggca acggtttggt
     4201 attcggtctg cctgtccaca ccgatgcggt gtttgacggt aacgggaatc ttgaccgcat
     4261 cctgcatggc gttgaggcag tcggcaacca gcccgacttc gttcatcaga cacgcgccga
     4321 acgcgccttt ctggacgcgc ggactggggc agccgcagtt gaggttgacc tcgttgtagc
     4381 cgtattcttc ggcggctttg gcggctttcg ccaaatcgga cgggtcgctg ccgcccagtt
     4441 gcagggcgac gggctgctcg ccttcgttaa acatcaaaaa gcggtctttg tcgccgtaaa
     4501 caatcgcgcc ggcgttgacc atttcgctgt acagccaagt attgcgggta atctggcgcg
     4561 ccatataacg gtagtgcctg tccgtccagt ccaacatcgg ggcgacggac aggcggcggg
     4621 gaggaagggt ttgcgtgttg tcgttcattg tcgatatttc accattgcgg attcaatttc
     4681 aatccggaac aaccttcatt tttggggaaa tccgcgtcat gaaagagtcc agcagtatgg
     4741 ttaccggccc gtcgttgcag agcgacacct gcatatgcgt gcggaaacgc cctgtttcga
     4801 catgaattcc gtgtccgcgc aacagttccg ccgttcgcag gtaaagctgc tgcgcctgtt
     4861 ctgcaggtgc ggcttgcgaa aacgaaggcc gccgcccgct tgtcgcgtcg gcataaagtg
     4921 taaactgcga caccagcagc accgcgccgc cgacatcttt caaagacagg ttcagcttgc
     4981 ccgcttcgtc ttcaaacacg cgcaaatggg cgattttgtc ggcgatatag tgcgcatctt
     5041 tttctgtgtc gctatgcgtt acgccgagta acacgacaaa cccgccgtcg attttgccac
     5101 aggtttccgt gccggcttcg gacacgacat ccacctttgc acctaccgtt ttctgtatga
     5161 ccgcacgcat aagccgtcct ccttacctga acgtgcaatg ccgtctgaaa aggcttcaga
     5221 cggcatcggt attggaaatg ccattacggt ttgacaccgt tttcttccaa aatggcatcg
     5281 attttcaagc gggctttttc ctgtctcaaa ccttgttcca actggtttct gaccaactcg
     5341 aaaggctgcg cgtcggggtt tttcccgacc tcgctgagtt tgaacagata atagcgttcg
     5401 cccaatttga ccggatcgcg ggtaacgtcg ccccgattca tcgctgcaaa ctgcgaagcc
     5461 agcggctcgg gaagctgctg cgccataatg aaaccgtcaa aagcctgctc gtcgttcgga
     5521 taacgcttca tcagcccttc aaaagacagc cctttgagca ggagctgatg cgcctgacgc
     5581 gcctcctctt cggttgcgaa gctgacctgc tgcaatttga tcatgcggat ttgccgctca
     5641 taaaactgac gcagtgcgct ttcggaaacc gtttccgaac gttccagaaa acggacgtac
     5701 tcctcggcat aaaaagacgc ttcggcgatt ttaaagcggt tttggacatc cttatcctta
     5761 tccaaacctt ccttcaatgc cctgtttttc aaaacttcca aagtttgcag ccgacggacg
     5821 gcatcgtttc ggattgcctg cccgtccggt ttttgggact gctccgcatg ccggtctgcc
     5881 tgctgcatga tctgcgccac cagcgtatcc accaaagccg ggtcgatttc gggtgctttg
     5941 gctgccgcaa aacctgccaa cattgcagca ataactgcgg cagcggtttt tttctgtttc
     6001 atggtattcc ctgtcctgcg cggcggcaaa acgcctgtct gaacgtcttg ccgccatatc
     6061 ccgttttcag aattattttg caggtttgat gtttgccttg cccaacagtg caccgacggc
     6121 acggtcaatc cgttccgcct gaaggttgcc ggcaatctgt cctttcattt catcaaaaga
     6181 aggcactttt acctcgcggc tgtcgttgac ataataaacg ccgtagaaat cgccgttttt
     6241 cagcggcgtt gccgtaaatt cgcctttttt caagtcctta attgcctgat aaagcggcgg
     6301 aacaccctgt tccaaatctt tcagcggcac atatccgacc ggcgcgccgg tctgtttggt
     6361 acggtcgttg agggaatatt gtttcaagac ggcatcgaaa ccttttttcg ccttcaagtc
     6421 ggcaaccgct ttttttgcat tttcttcctt gtcggtcagg atttcgccca actggacttc
     6481 ctgcgtacct ttgtaaaaac cgctgatatt gtcatatgcg gcttttactt cctgctcgga
     6541 aaccggttgg gttttggcga tatgcagcgc gtatgcctcg ccgttcaagc catattttac
     6601 cgcctgccaa acggttttga aggacggttt cttgtcgtcg cccgactttt tcgcttcggc
     6661 acgcaatttg gcaagcgcat ttttaaactc tgccgaccgg tcgagtttca gacgtttcac
     6721 ttcctgtgcg accacggtgt tgaccacttc gttttccagc agggattggc gcagttgcgg
     6781 cgtgtcttcg gcacggctgt tttccgcacg gaatgcggca acctgcgcat cgatgacgga
     6841 actgtcgatt ttctgaccgt tgacggttgc cagcgtttgg gcaaacaggc tgccggaaca
     6901 ggcaagcagt gcaacggaag tcaggatttt tgctttcata atcttctctc tttaaatttt
     6961 aaattgaaat gttcgggcag acttcattca gtccgccgta tggaaatact cgtcaggggt
     7021 agccgcccgg atgccgagcg cgtgaatcat gccgcctgaa aacaaatctt tgagcagcga
     7081 tttgaccgtt ttctggcggt tcaggcggct tacgccttca aaacggccgc tgacgaccaa
     7141 aacggcataa tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat ggctctcatc
     7201 gccgatttcc aacaccagcg gatcgagcgt ctgcaggcgt tcgcggatca aatcgacggc
     7261 cggcattatt tgaacaccgc tttaaacggc ttgatcagca cttcgctgta cacgcctgca
     7321 tgaacatagg gatcgtcttc cgcccacgcc tctgccgcat ccaaagactc gaactgcgcc
     7381 acaatcaaac tgcccgaaac gcggttggag tcctccggca aaggattcgg gcctgccgtc
     7441 aacagccggc cttccgactt cagcgtctcc agccgtttga ggtgttcggg acgtgccgcc
     7501 atacgcgctt catacacatt ctccccgtct gttgccagca acataaaata ttccacagtc
     7561 aatcctcctt tttcagacag gtactcagat aaataccctg aataataaag aaaacaagca
     7621 tcagtgcagt cgagccgaac attttatagt tgacccattg cgcttcaaac ctagtaaaca
     7681 caaaccagtt ggcaataccc ataaaaatca gaaaaccgac ccacatatac gtcaatttcg
     7741 cccatacggc atctgaaagc tgaatctccc tgccgatact cgccttcaag ccgtttttac
     7801 cggcgaggtg gctgccccac aggaacagcg cgcccagcca aaacaaaacg ctcggcttcc
     7861 acataatgaa gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacc acaatcagca
     7921 ccaatccgac ccactgcatc gtatccagct ttttatattt ccaatacaga aaagccgcct
     7981 gaaccacacc ggcaaccaat gcgaccgccg ttgcggcaat catgtttttg gtaacggtat
     8041 aggtggcgaa aaacaggatg acggacaaaa ggtcgctgac gaatttcata tccaaatgag
     8101 aaagcaaatc aaataaacgc taatcataca ggcagaaccc tgtttttgca cctgcaaaac
     8161 caacaaattt tgttttgatt gatgcaaata tgcacagatg tttttgaaaa aagatggaga
     8221 tatgtcataa tttgtaaaaa cggcgaatga ttgccgttta aaatgtggcg gcggtcggta
     8281 cattcacata ttaaatgccc gccggccgac tgccccgccg atgcccgatg atgacgaaat
     8341 ttacagactg tacgcggtca aaccgtattc agccgccaac ccacagggga tacatcttga
     8401 aaaacaacag acaaatcaaa ctgattgccg cctccgtcgc agttgccgca tcctttcagg
     8461 cacatgctgg actgggcgga ctgaatatcc agtccaacct tgacgaaccc ttttccggca
     8521 gcattaccgt aaccggcgaa gaagccaaag ccctgctagg cggcggcagc gttaccgttt
     8581 ccgaaaaagg cctgaccgcc aaagtccaca agttgggcga caaagccgtc attgccgttt
     8641 cttccgaaca ggcagtccgc gatcccgtcc tggtgttccg catcggcgca ggcgcacagg
     8701 tacgcgaata caccgccatc ctcgatcctg tcggctactc gcccaaaacc aaatctgcac
     8761 tttcagacgg caagacacac cgcaaaaccg ctccgacagc agagtcccaa gaaaatcaaa
     8821 acgccaaagc cctccgcaaa accgataaaa aagacagcgc gaacgcagcc gtcaaaccgg
     8881 cgtacaacgg caaaacccat accgtccgca aaggcgaaac ggtcaaacag attgccgccg
     8941 ccatccgccc gaaacacctg acgctcgaac aggttgccga tgcgctgctg aaggcaaacc
     9001 caaatgtttc cgcacacggc agactgcgtg cgggcagcgt gcttcacatt ccgaatctga
     9061 acaggatcaa agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc gaaaccgcat
     9121 ccatgccgtc cgaaccgtcc aaacaggcaa cggtagagaa accggttgaa aaacctgaag
     9181 caaaagttgc cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc gaacctgtac
     9241 ccgctgcaaa tactgccgca tcggaaaccg ctgccgaatc cgccccccaa gaagccgccg
     9301 cttctgccat cgacacgccg accgacgaaa ccggtaacgc cgtttccgaa cctgtcgaac
     9361 aggtttctgc cgaagaagaa accgaaagcg gactgtttga cggtctgttc ggcggttcgt
     9421 acaccttgct gcttgccggc ggaggcgcgg cattgatcgc cctgctgctg cttttgcgcc
     9481 ttgcccaatc caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa gagcctgacc
     9541 ttgacgacgc ggcagacgac ggcatagaaa tcacctttgc cgaagtcgaa actccggcaa
     9601 cgcccgaacc cgctccgaaa aacgatgtaa acgacacact tgccttagat ggggaatctg
     9661 aagaagagtt atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct tccaaccgca
     9721 tcgacttgga tttcgacagc ctggcagccg cgcaaaacgg cattttatcc ggcgcactta
     9781 cgcaggatga agaaacccaa aaacgcgcgg atgccgattg gaacgccatc gaatccacag
     9841 acagcgtgta cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc gtcatcgaca
     9901 cgcccgaacc ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc gtcgataccg
     9961 atttctccga caacctgccc tcaaacaacc atatcggcac agaagaaaca gcttccgcaa
    10021 aacctgcctc accctccgga ctggcaggct tcctgaaggc ttcctcgccc gaaaccatct
    10081 tggaaaaaac agttgccgaa gtccaaacac cggaagagtt gcacgatttc ctgaaagtgt
    10141 acgaaaccga tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac gccgccgcag
    10201 acgatttgtc cgcattgctt caacctgccg aagcaccgtc cgttgaggaa aatgtaacgg
    10261 aaaccgttgc cgaaacaccc gatttcaacg ccaccgcaga cgatttgtcc gcattacttc
    10321 aaccttctga agtacctgcc gttgaggaaa atgcagcgga aatcgttgcc gatgatttgt
    10381 ccgcactgtt gcaacctgct gaagcaccgg ccgttgagga aaatgtaacg aaaaccgttg
    10441 ccgaaatacc tgatttcaac gccaccgcag acgatttgtc cgcattactt caaccttctg
    10501 aagtacctgc cgttgaggaa aatgcagcgg aaaccgttgc cgatgatttg tccgcactgt
    10561 tgcaacctgc tgaagcaccg gccgttgagg aaaatgcagc ggaaatcact ttggaaacgc
    10621 ctgattccaa cacctctgaa gcagacgctt tgcccgactt cctgaaagac ggcgaggagg
    10681 aaacggtaga ttggagcatc tacctctcgg aagaaaatat cccaaataat gcagatacca
    10741 gtttcccttc ggaatctgta ggttctgacg cgccttccga agcgaaatac gaccttgccg
    10801 aaatgtatct cgaaatcggc gaccgcgatg ccgctgccga gacagtgcag aaattgctgg
    10861 aagaagcgga aggcgacgta ctcaaacgtg cccaagcatt ggcgcaggaa ttgggtattt
    10921 gattcccaac tgccctttcg cagatcaagg atgccgtttc agacggcatc tttttttgcc
    10981 ttatcggtgt aacggataaa gtttgaaccg gcacaggctc aaacagcagg ccgacggcaa
    11041 caaaatgccg tctgaaaccc ctaaaggctt cagacggcat tggcggcggc ggtatcaggc
    11101 agtttgatag gcaaccgaat cgtcaccgct tttcttttga atgaactcga ttttgtatcc
    11161 gtcggggtct tcgacgaagg ctatcacggt tgtgccgtgt ttcatcgggc cggcttcgcg
    11221 gacgacgttt ccgccctgcc gcttcacacg ttcgcaggct tcgtaggcat cgtccacttc
    11281 aaccgcgatg tgtccgtagg cgttgcccaa gtcgtatcgt tccgtatccc agttgtgcgt
    11341 cagttccaaa accgtgctgt cggtttcgtc gccgtaaccg acgaaggcaa gggtaaatct
    11401 gccttcggga taatcttttc ggcggagcag tttcataccc aaaacgtttt ggtagaaatc
    11461 gagggatttt tcgagattgc ccacgcggag catagtatgg agtaagcgca tttttgtgtt
    11521 cctctcggta gtggttaaac ttcgattttg tttggcgtaa acgtctgcca tttgttgcag
    11581 gcaggacaat gccagaaaaa gacttgtgat ttgaagtggc agtttcggca ccggtacatc
    11641 acgctgcgct gtagctgccg tccgataacc gaacgcatca tatcggcatc ggctttccaa
    11701 gccggatcca aatcgctgag tttcaaacca agcaggcggt acacgccgtt gaggtcgggc
    11761 ttgcggcgga caagctcgac ggcggtttgc gcggcttctt tctcgcactt aagcagcagg
    11821 gatttctcgt acacgacatt gatcaggtca agttcgggaa acgtctgcat atatcctgtc
    11881 agacggttca agccttcttc aggttttccc tgcgcggcat aggcttcgta aagcttctcg
    11941 ccgaccatgc tcaagtatgc atggttttgc tgctcgatgg cggcataggc ttcgacggcg
    12001 gcagggaaat tgccttgtcg gtgttcgatg tcgcccaaaa tcatgttggc acgggtgcat
    12061 tttttgttgg cttcgagtgc cttgccgaca ttgaaacgcg cgacatcgaa attggacttg
    12121 aacagcgcgg cttgggcaag ttcgcaataa aactgggcga tttcaaactg ataggtctga
    12181 tcgtcatggc tgagcagccg ggcggtttca accgcttttt cccaatccct gtcctgttgg
    12241 tagatattga gcaggtgctg tctggcttca cgcgccattt taccgtcttg cagccccaaa
    12301 aaaatctgtt cggcacgatc gaccaacccc gcactttggt agttttgcgc caattcaaac
    12361 aggacgcgcg cgcgcttttc gccgaccgta tcgggagaat cgagcattgt ccggtgtatg
    12421 ttgatggctt tgtcgttttc gccacgctga cggtaaagct tgcctagggt gaggtttagg
    12481 tcgtatgact gaggctgctg atcaatgacc tctgccaatt ccctggcggc acgtccgctg
    12541 ttgcggtcaa ccaaggcatc cagactttta taaaatcccg acggtatgct ttttgcctgc
    12601 tttaatacag tcttcatatc cacacgggcg gcaaaccagc ccatcgcgaa gaaaacgggc
    12661 aaaaggataa tcggcagcag gataatccac aattcattgt ccatatcggc tttctcaagg
    12721 ctgtttggca gattcgggcg cattttgcgc cggtggtgcg gtcagctctt tccccgtcaa
    12781 acgcgcattt ttctttactt cggcacgcaa cctgccgttc tcgccacgta acgacaacaa
    12841 ccgtccgaac aaggcaaaca ttccgaacac gatgccgacg acaaacgcgc cgaacaatac
    12901 gacaatcagc ggcaaatcga ctttctgtcc gggcaggtag gagaaggtaa cggcatccgt
    12961 attaatgacg gcaagcagca ggaagaacag caggataatg attttgacga cggtatagat
    13021 aagtttcatg tttgctccgt aaataaaaac gccgcccgga cagcttcgca ctctgaacca
    13081 agattgcgct agtttaaacg atttgcacgg tttcggatag gatgcggcag cgtgttcgga
    13141 cgacatacgg agtatgcgat tgccgacaat tttaccaaat acaccgttcc tttccatttg
    13201 aaaaataacg gattggacac cgcatcgaca gaaaaacccg ccgcgcactt gtcaaaaccc
    13261 tgtttgcagg cgtatcttta caatcttcaa attcaaaccg ttcattgaaa catatcagaa
    13321 taagaaaggc tttacatcat gagcagaccc gtacccgccg tattcggcag cgtttttcac
    13381 agtcaaatgc ccgtcctcgc ctaccgcgaa ggcaaatggc agccgaccga atggcaatct
    13441 tcccaagacc tctccctcgc accgggtgcg cacgccctgc actacggcag cgaatgtttc
    13501 gagggactga aagccttccg tcaggcagac ggcaaaatcg tactgttccg tccgactgcc
    13561 aacatcgcgc gtatgcggca aagtgcggac attttgcacc tgccgcgccc cgaaacccaa
    13621 gcctatctcg acgcgctaat cgaattggtc aaacgcgccg ccgatgaaat tcccgatgcg
    13681 cctgccgccc tgtacctgcg tccgacctta atcggtaccg atcccgttat cggcaaggcc
    13741 ggttctcctt ccgaaaccgc cctgctgtat attttggctt cccccgtcgg cgactatttc
    13801 aaagtcggat cgcccgtcaa aattttggtg gaaaccgaac acatccgctg cgccccgcat
    13861 atgggccgcg tcaaatgcgg cggcaactac gcttccgcca tgcactgggt gctgaaggcg
    13921 aaagccgaat acggcgcaaa ccaagtcctc ttctgcccga acggcgacgt gcaggaaacc
    13981 ggcgcgtcca actttatcct gattaacggc gatgaaatca ttaccaaacc gctgaccgac
    14041 gaatttttgc acggcgtaac ccgcgattcc gtactgacgg ttgccaaaga tttgggctat
    14101 accgtcagcg aacgcaattt cacggttgac gaactcaaag ctgcggtgga aaacggtgcg
    14161 gaggcgattc tgaccggtac ggcagccgtc atctcgcccg ttacttcctt cgtcatcggc
    14221 ggcaaagaaa tcgaagtgaa aagccaagaa cgcggctatg ccatccgtaa ggcgattacc
    14281 gacatccagt atggtttggc ggaagacaaa tacggctggc tggttgaagt gtgctgatgc
    14341 tttaaataaa aaatgccgtc tgaaacccgt ttggcgtttc agacggcatt ttcgcatccg
    14401 aaccgtttcc gctgcacctg cagcaagtcg gcacaaaggc aatcggttaa aacaagcgtc
    14461 cgcatttctc atcccgcctg ccgcaagtcg ggtatttctc tggaaatacg cttcagacgg
    14521 caaaacgctg cccgaaaccg atacgcggca cggacggcgc acagatttga aaacagcgga
    14581 ttatccctcg gtgctcaagg cattgatgct gtaaccgccg tcaacgtaag taatttcgcc
    14641 ggtaataccg gacgacaggt cggacagcag gaaggcggcg gtattgccga cttcttcgat
    14701 ggtaacgttg cggcggaggg gattgtgggc ggcgacgtgt cccaagagtt tgccgaaatc
    14761 ggcgatgccg gaggcggcaa gcgttttgat cgggccggcg gaaatgccgt tgcagcggat
    14821 gccttctttg cccaaacagg cggcggtaaa gcggatgcct gcctcaaggc tggcttttgc
    14881 catacccatc acgttgtagt tcggaatcgc gcgcaccgcg cccaagtagc tcagggcgac
    14941 gatggcggag tttctgccgc gcatcatcgg acgggcggct tttgccagcg caggcaggct
    15001 gtatgcggaa atttcgtgtg cggtgttgaa cgcttcgcgg ctgatgctgt cgaggaaatc
    15061 gccgctcaag gcttctttcg gcgcgaagcc gatggaatgc accaaaccgt ccaagccgtc
    15121 ccaatgcttg cccaagtcgg cgaacacttg gttgatttcg tcatcgctgg cgacatcgca
    15181 gcggaatacg agttcggaat ccaattccgc cgccattttg cggacgcgct cttccagttt
    15241 gtccacaacg taggtaaacg ccagttccgc gccttgttcg cggcaggctt tggcgatgcc
    15301 gtaagcgatg gaacgctcgg aaatcatgcc ggtaatcaga atttttttgc cttgcagaaa
    15361 acccattttg tatccttcaa atagtgttcg gttcgttcta aaccgttgat tatagcaaat
    15421 tgtccctgtt tctgtgtttt cacgttgcag cgtgcaaacg gcaatgccgt ccgaagcgga
    15481 tttcagacgg catcggacgt ttcaaatacg gtttaaggca tcagatgccg cgcaacaatt
    15541 cgttcacgct ggttttcgca cgggtttgcg cgtccacacg tttgacgatg acggcgcagt
    15601 aaaggctgtg gctgccgtct ttggaaggca tactgccgga cacgacgacc gaaccggccg
    15661 gtacgcggcc ttggtagatt tcgccggtgg tgcggtcgaa gattttggtg gattgaccga
    15721 tgaacacgcc catcgaaatc acgctgcctt cttcgacaat cacgccctca acgatttcag
    15781 aacgcgcacc gatgaagcag ttgtcttcaa taatggtcgg gctggcttgc aggggttcga
    15841 gtacgccacc gatgccgaca ccgccactca agtgcacgtt tttaccgatt tgcgcgcaag
    15901 agccgacagt cgcccaagta tcaaccatcg cgccttcgtc gacgtatgcg ccgatgttga
    15961 cgtaagacgg catcagcacg acatttttcg ccacaaagct gccgcgtcgg gcaaccgcac
    16021 ccggaacggc gcggaagcct gcgtttttga actcgtcttc agaccagtcg gcaaacttgg
    16081 tcggcacttt gtcgaagtat ttgttcacgc cgtcgttgag gacttcgttg tcttggatgc
    16141 ggaaggacag caacacggct tttttcgccc attcgttgac tttccactcg cccacgccca
    16201 agcgttcggc aacgcgcagt ttgcctgaat cgagttggcg gatggtttcc aacacggctt
    16261 ctttaacttc gggagcaaca gtggacgggg tgatgtccgc gcggttttca aaggcggttt
    16321 cgataatgtt ttgcaaagac ataatgtttc cttatgtgag atgtttcgtg ggacgcttgc
    16381 cggtattttc aagcagtatg ttcatttgaa atgaatgcgc tattgtgcca cgcaaacgca
    16441 tattcgtcag caatacgcgg gcggttgccg aacattggcg tttcggaatc gggcgggcgg
    16501 caggtattgc cttttccgca cccgaatcaa tacagaacgg cggcggtact tttatgccgc
    16561 gccgccgcct ttcagacggc attcacggta aaacgccatc agcccttcgg tcgaggcatc
    16621 gtgtacggac gtgccgcctt ccagttcgcc gatgatggtt ttcgccaact gtttgccgta
    16681 ttccaccccc cactgatcga agggattgat gtcccaaatt acaccctgca caaaggtttt
    16741 gtgttcgtaa gccgccatca gcatacccag attgtagggc gtgaggcgtt cgagcaggat
    16801 gctgttgctg gggcggttgc cggggaactc tttgtgcggc gcgaggcgtt cgcgttccgc
    16861 ttcgggcaaa tctgccagtt cggcgcgtgc ttcgtccaag gttttgccct tcatcagggc
    16921 ttccgcttgg gcaaaggcgt tggcaacggt aaaacggctg cgtccgtcct ccacgccctg
    16981 cgccgtcatc gggacgataa agtcgcaggg aatcaggcgc gtgccttgat gaagcagttg
    17041 gaaataggcg tgctggcagt tgaccccttc accgccgaac acgatgccgc ccgttttgca
    17101 cacggcggga ctgccgtctg aagcgcggct tttgcccaaa ctctccatat cgagctggtt
    17161 cagccacacc ggcagcaggc gcaggttgtg gctgtacgga acggcggtct gcccgtccgc
    17221 gtgttggaaa ttgttatacc acacggcaat cagtgccatc agaacgggaa tgttgcggcg
    17281 cggcggcgtg tggaaaaaat ggctgtccat cgcgtgcgcc cccgccaaca actcgcggaa
    17341 acgcgccccg ccgaccgcaa ccatcacggg caaaccgacg ggcgaccaga cggaatagcg
    17401 tccgcccacc cagtcgtaca tcgcaaacac gcgttccgcc gcgataccaa aagccgcagc
    17461 tgccgcagtg tcggcagaca ccgcgcaaaa atggcacgcc gtttcggatt ccgagaaccc
    17521 cgcaccgcga taccacgcct tgactgcctg tgcattgagc agggtttccg gtgttttgaa
    17581 ggacttgctg gcaacgcaaa acactgtcgt ttcggggttc agacggcata aaaccgcatc
    17641 caggcaggca ggatcggcgt tggcggcaaa atggacggcg atatgccgtc tgaacggctc
    17701 aagtgcctgc acgcacattg ccggcccgag gtcggatccg cctatgccga tgtggacaaa
    17761 atccgtaatc cgttttccgg ttgtcccctg atacgaaccg tcgtccaaac tgtgtgcaaa
    17821 cttcaacgca cgatttaact cgcggcggat ttcgggcaac acgtccctgc cgtccgcata
    17881 aacggcaccc gcaccgtcgg gcaggcgcaa agccgtatgc agcgcggcac gcccctcact
    17941 gttgttgacc ttcgcacccg tccgcaaagc acgcattttc ccttccaaat ccgccgcatc
    18001 ggcaagattg cagagcagtt gcagcgtatc ttcgcccaaa cggtttttgc tgtaatcgaa
    18061 caacatcccg tccaaacgct cgtgcatacg ctcaaaacgg tcgggttcgg cagcaaaacg
    18121 gtcgcgcaaa agaatgtggc gcgtatcctg ataatggcgt tcgagcgcac accatgcacg
    18181 ggtaaaagca tccatctgtt ttccttgatt tttcagaacc ggattaaaat gtagtagaat
    18241 gtagtttaac aaacggcagc ggctttggcg aatctccgga acaccgcccg ccgcgtccga
    18301 tagcacctta tttccatatg gcgaaaatat agtgaattaa caaaaatcag gacaaggcgg
    18361 cgagccgcaa acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
    18421 gaatcgttct ctcttgagct aaggcgagcc aacgctgtac cggtttttgt taattcacta
    18481 taccctccgg ccggcatttg acccgcaaca atatcctgca agatttattg tgtacgcata
    18541 aatgccggac agccgcctaa ataaggtata atcgcatccg attctgtccc gtttgccgcc
    18601 cgcaggcggg atgtcggcgg ttttcatctt accgggaaca cgccggtact gttttttaac
    18661 gatgtttctt acattttatt ccaattactt tacggggcta gaatatggct aaaaacggag
    18721 gattttcttt gttcgcaaag aaagaaaaac gctttatctt tgaaggcagg cattccgcct
    18781 ccgacaaact ggtcaacggc gaagtatccg cgtttaccga agaagaggcg cgcaaaaaac
    18841 tggcaaaacg cggcatccgc ccgttgcaga ttacccgtgt gaaaacaagc tccaagcgca
    18901 aaatcacaca agaagacatc accgttttca cccgccagct gtccacgatg attaaagcgg
    18961 gcctgccgct gatgcaggca tttgaaatcg tggcgcgcgg acacggcaac ccgtctatga
    19021 cggaaatgct gatggaaatc cgaggcgaag tggaacaggg cagctcgttg agccgcgcat
    19081 tctcaaacca cccaaaatat ttcgaccgct tctactgcaa tctggttgcg gcgggcgaaa
    19141 cgggcggcgt attggaaagc ctgctggaca aattggcaat ttacaaagaa aaaacccagg
    19201 ccatccgcaa aaaggtaaaa accgcactga cctatccggt atccgtcatc gccgtcgcca
    19261 tcggtttggt attcgtgatg atgattttcg tactgcccgc ctttaaagaa gtttacgcca
    19321 atatgggcgc ggagcttccc gcactgaccc aaacagtgat ggatatgtcc gactttttcg
    19381 tctcatacgg ctggatggtg ctgatcgcac tgggctttgc catatacggc ttccttaaat
    19441 tgaaggcgcg ttcgattaaa atccaacggc gtatggatgc catactgctg cgtatgccga
    19501 ttttcggaga cattgtccgc aaagggacga ttgcccgctg gggcaggacg acggcgacgc
    19561 tgattgcggc aggcgtgcct ttggtcgatg tattggactc cactgccggc gcggcgggca
    19621 atttaatcta tgaagaagcc acccgggaaa tccgtacgcg cgtcatccaa ggtttgtcta
    19681 tgacttcggg gatgcgtgcg acggaactgt tccccaatat gatgttgcag atgtcttcca
    19741 tcggcgagga atcgggttct ttggacgata tgctcaacaa agccgccgaa ttttacgaag
    19801 acgaggtgga caatgcggtc ggcaggctgt ccgctatgat ggagccgatc attatcgtga
    19861 ttttaggctt ggtcatcggc acgcttctgg tcgccatgta tctgccgctg tttaatttgg
    19921 gcaacgtggt cgcctgattt gccgcacaga tccggcgcgg attcgctctg cgccggtttg
    19981 tttttgtttt gaatacattg agaacaaaat atgtctgatt tgtctgtatt gtcgccgttt
    20041 gccgtgcctt tgacagcggt gttcgggctg ctggtcggaa gtttcttaaa tgtcgtcatt
    20101 taccgcgtgc cggtcatgat ggaacgcggc tggacggtat ttgccaaaga atatttaaac
    20161 ctgccgctga ccgaagagga aagccgtacc ttcaacctga tgaaaccgga ttcctgctgt
    20221 cccaaatgcc gcgtgccgat acgcgcgtgg cagaacatcc cgattgtcag ctacctgctc
    20281 ctgcgcggca aatgcgcctc ctgccaaacc aaaatcagca tacgctatcc cttaatcgaa
    20341 ctgctgaccg gcgtattgtt cgggctggtt gcctggcaat acggctggtc ttggattaca
    20401 ttgggcggtt tgatactgac cgcgtttctg atttccctga cctttatcga tgcggacacc
    20461 caatacctgc ccgactcgat gacattaccc ttgatttggc tggggctgat atttaatttg
    20521 gacggcggct tcgtgccttt gcagtctgcc gttttaggtg cggttgccgg ctatggttcg
    20581 atatggcttt tatgcgcggt atataaactg attaaaggcg aaatcggtat gggcaacgga
    20641 gatttcaaac tgattgccgc attgggcgcg tggctcggca tatccgcatt gcccgtgctg
    20701 atttttgttt cctcgctgat cggtttggtc gcggcaatcg ttatgcgcgt cgccaagggg
    20761 cagcattttg ccttcggccc cgcactgaca gtttcgggct ggataatttt tacggcaaac
    20821 gattccgtat ggcgggcggt caactggtgg ctgacccatc cggtgctgta agatgacggt
    20881 atgggtcgga ctgaccggcg gaatcggcag cggcaaatcg gcagccgcgc aatgttttgc
    20941 cgatttgggc gtaccgcgta tcgatgcgga tgcggcggcg cactcgctga cggcttcaga
    21001 cggcatcgcc ctgccggaaa tcaggcggct gttcggcgac accgttttcg acacacaggg
    21061 tttgttgcga cgcgacatat tgcgtaaaga agtctttgcc tccccatcgc gaaaagcctt
    21121 gctcgaatcc gtgatgttgc cgctgatttt ctcagaaatc aaaaaacagc aagaaacctt
    21181 taccgatgcg gtttacggca ttgtcgaaat tccgctgctg acggaaaagc gtcaatttat
    21241 cagcctgata cggcgtgtcc tgaccataag tgcccctgtg gaaaaacgta tcggcagggt
    21301 gatggcccgc agcgggctga cgcgcggcga ggtggcggcc gtcatcagcc atcaggcatc
    21361 cgaatccgaa cgcctgctgc ttgcagacga tgtgctgctc aatgacggca gcctcaaaag
    21421 cctgcgtgag aaaacaatgc gcctgcacgc gttttattca gggattttcg cctcaaaacc
    21481 aacacaagga aaacacaatg actgaatcgc ggcaaacacg ccttcaagtc aaatgtccga
    21541 cctgtcaaac ggcagtagta tggaaaccgg aaaacgcatt ccgccccttc tgttcgcaac
    21601 gctgcaaact gatcgacttg ggcggatggg cagacgggaa atatacggtt tccagccaaa
    21661 cggaaagttt gccggaaata tccgaacccg acatggcata ccgctgaccg ccccgccttc
    21721 ccggcaaaca ccctgaaagt caaatgccgt ctgaaacaaa cacgcttcag acggcatttt
    21781 cattctcaaa cctaatcgtt ggtatttgcc gttacctctt ccaatgaagt aatgccctgc
    21841 ataactttca aaataccggc ccggcgcaaa tccaccatac cctccttata ggcaacgtcc
    21901 aaaatatcca cttccgtacc gttgttcata atcacacgct gcatttcttc gctgatgggc
    21961 ataacctcat acacgcccgc acgcccttta taaccctgcc cccggcaacg gtcgcaaccg
    22021 acggcgcggt aaagtttcca atcttttgca aggtcctcat cggtgaagcc gacttccttc
    22081 aaagcagagg cagacgggcg ttccacttcc tgtttgcagc tcgaacacag cctgcgtaaa
    22141 agacgctgcg ccataatcag gctgaccgaa ctggcaatat taaacggcgc gacacccata
    22201 ttcagcatac gcgacaacgt cgccggcgca ttattggtgt gcagggtgga aaacaccata
    22261 tgccctgttt gtgccgcctt aatcgcaata tcggcagttt ccaaatcacg aatctcaccg
    22321 accataatga tgtccgggtc ctgacgcagg aaagacttca aagcagcggc aaaagtcagg
    22381 ccctgcttat cattgacgtt aacctgattg atgcccggca ggttaatctc ggcagggtct
    22441 tccgccgttg caatatttac cgactccgta ttcaaaatat tcaaacaggt atagagcgac
    22501 accgtcttac ccgaacccgt cggaccggtt accagcacca tcccgtaggg acggtgaatc
    22561 gcttccaaca acaatttttt ctgaaacggc tcaaaaccga gctggtcgat gttcaaagac
    22621 gcggcatcgg aattcaaaat ccgcatcacg accttttcgc caaacagcgt cggcaatgtg
    22681 ctgacacgga aatcgacagg cttgccgccc ttttgaaagg tcagctgcat cctgccgtcc
    22741 tgcggtatcc gtttttcgga aatgtccaaa cgcgacatta ccttaatccg ggaagcaagc
    22801 tgccccctta ccgcaatggg cggctgaacc acctcgcgga gctgcccgtc cacacggaaa
    22861 cggatacggg cattgtgttc gtaaaactcg aaatggatgt ccgatgcccc gctgcgcaag
    22921 gcatccgaca aagtcttatg gataaacctc ggaacagggc cgtcttctgc ctcctcgttg
    22981 tcgatataca gggtgtggct ttcctcttcc tcctgcccct ccccaagctc ctgaagcagc
    23041 gatgtcgaac gcgaacccac ccaatcgagc aaacccgcca actggtcatc ctcgacaatg
    23101 accaattcaa cctcaatccc tgcggcagaa acggttttct gaatttgcgg catctgtgtc
    23161 ggatcggaaa ccgcaaaaaa tactttgtcg ccccgacgga aaaccggcac acagtggaac
    23221 tccaccatct gctcctccgt caacaccccc atcagcaccc tgtggcgcgg ataatgacgc
    23281 aaatcaagaa tcgaataact gaacaccctc gcaatcaatg ccgcaagcga cttgggcgaa
    23341 atgacaccgt ctgaaaacag catcggcaac acttccttac ccgcctgcga ctcattgtag
    23401 taatgctcgg cctgctcaac agtaaccacc tggttttgaa ccagaatcct cagcaaacct
    23461 acgctcatac gaccttatcc ccaaatttat tcattgttat acctgtacag cttttatagt
    23521 ggattaaatt taaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta
    23581 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg
    23641 ccttgtcctg atttaaattt aatccactat atcataaaac aacagatttc caagccggaa
    23701 catcttttac ggagcctgaa aatcatttca tgattctacc gtcctaaagg tcggtttttc
    23761 aagcaggaag aaaaagtttt cagatggcaa aaaagccctc caacactgaa aggccttata
    23821 tcggaaactt cccgcaacac gggaaacaga caaatgcaaa tcgtcaaacc tcgccaacag
    23881 gaatcgaacc tgtattttac gcttaggagg catacgttct atccgttgaa ctatggcgag
    23941 ccgaaatgaa aaggagattt taaccctttc cgacgacaaa agacaagatg ccggccgcaa
    24001 tcagcggccc gacagggata ccgcccataa atgccacccc gataaccgtc ccgattaaca
    24061 gccctgtaat taaaacaggc tgctgtccca tcaaaggcac gccgcgtccc gccagccaag
    24121 ccacgaaaat accgataaaa acggcggata tcattttaaa attcaaaaat tcggcaacgg
    24181 gaggaacctg cgcctttcct gaaaccaacg gactcaaaac ccctatggtc aaaagaatga
    24241 taccgagatt caacccatgc ttctcgacca acgggacaaa ctgtaccaat gccgtctgct
    24301 gcatcagcag caatatggtt gccgagatgg taatcgaatt gttgttgctg accaccccca
    24361 acagaatcag cgtaaccaga aacaggggaa caaaactgaa attcatcgcc tcaagacacc
    24421 ctgaccaaag attccttcgc cagctgaatc atggcatcct tcacttcgct gtcgggcagg
    24481 gcatccaagg cggtaacggc acaatcgact gctttgcgcg cctcgcctat cgaatatgcc
    24541 aaagcatccg aacggacgac ataatcgtgg attttctcaa aatagctgcg atctgcattt
    24601 tccaaagcag tacgcacatc gttcgcaacc tgttcggaac cctgacgcat cagataaatc
    24661 aaaggcaaag tcggttttcc ttccgccaaa tcgtcgccga cgtttttgcc ggtttcttcg
    24721 gtttcgccag aatagtccag cacatcgtca ataatttgga atgccgtacc gacatacata
    24781 ccgtagtctt tcaacgcccg ttcgtgttcg ggggaagcct tgcccaaaat tgcgccgact
    24841 tgagcggcag cttcaaacaa ttttgccgtt ttatattgga tgacttggat atattgttct
    24901 tcggtaatgt ccgtattgcc gatgttcatc agctgcatga cttcgccctc ggcaataatg
    24961 ttggttgcat ccgccatcac ttccaaaacg cgcatactgc ccgaggcaac catcagttga
    25021 aaggcgcgcg tgtataaaaa gtcgccaacc aacacagccg ccgcattgcc gaacagattg
    25081 tttgccgttg cccgcccacg gcgcaaatcg ctttcatcga cgacatcgtc gtgcaggagg
    25141 gtggaagtgt ggataaactc gaccatcgcc gccagcgaat acagtttctc gtcatcataa
    25201 ccgaccgcct tacccgccaa aatcgtcata atcggacgca ggcgtttgcc gcccgcgctg
    25261 atgatatatg taccgatttg cgaaatcagt gcgacatcgg attgcaccgc acggttgatg
    25321 acttcattga ctttggcaag gtcttcaggc agatgtcgct ggaaataggg cagattctcg
    25381 agcatggcag actctcaggt ttacgtttcc gtcaaacggc agataccgct tgaaagctga
    25441 ttcaaatagc gggcaattat atcagcaaag ccatcagggt acatcttttc agacggcata
    25501 tggttatccg aaccgcgcaa actttgacaa aacaggcaaa taacaataca atgccgagtt
    25561 ccgcacaatg gtgtgcggat attaaccata acccttatgg agttgagtat gtacgcggtc
    25621 gtaaaaaccg gcggcaaaca gtataaagtt tccgtcggcg aaaaattgaa agtagaacag
    25681 ataccagccg aactcgacag ccaaatcgaa ctgaccgaag ttttgatgat tgctgacggc
    25741 gaatctgtaa aagttggcgc accctttatc gaaggtgcaa aagtaacggc taaagtagtg
    25801 gcacacggtc gtggcgaaaa agtccgcatc ttcaaaatgc gccgccgcaa acactaccaa
    25861 aaacgccaag gccaccgcca aaatttcacc caaatcgaaa tcgtggcaat cgcctaacct
    25921 taaaacttag gagcattaca aatggcaagt aaaaaagcag gcggcagcac ccgcaacggt
    25981 cgcgattcag aagccaaacg cttgggcgtt aaagcctacg gcaacgagct gattcccgca
    26041 ggttccatca tcgtacgcca acgcggtacc aaattccacg caggcgacaa cgtaggtatg
    26101 ggcaaagacc acactttgtt cgccaaagtt gacggttatg tcgaattcaa aaccaaaggc
    26161 gcgctgaacc gtaaaactgt cagcatccgt ccttacaccg gttctgaaga ataatccaac
    26221 cgatacctga agccgcatct tttcacgatg cggcttttta tagtggatta acaaaaatca
    26281 ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgccgta
    26341 ctggtttaaa tttaatccac tatacatacc ccacacccga tttgatacac attaagctga
    26401 aagtaaaaat ccgcatacac cctcccctgc atatttcttc aacagtgggt tttgatataa
    26461 tcgcctatct gttacagata gttcaaacgg cattcaaacc cttacaaata ccgtctgaaa
    26521 tcatcgacat ccgtctatgc aaaggatatt ttatgagttt acacagcgac atcctcgtcg
    26581 tcggcgcagg cccggcgggt ttaagttttg cagccgaact tgccggcagc ggtttgaaag
    26641 ttaccctgat cgaatgcagc ccactgactg ttctgcaaaa tcctccatat gacggacgcg
    26701 aaatcgccct gacccatttt tcccgcgaaa tcatgcagcg tttgggtatg tgggacaaaa
    26761 ttcccgaaaa cgaaatctac cctttgcgcg atgccaaagt gctgaacgga cgttccgact
    26821 accagctcca ctttccccaa ccgaccgagg cgcgcggcga gcctgccgac tgtttgggct
    26881 atctgatttc caaccacaat atccgccgcg ctgcctacga agtcgtatcc caactcgaca
    26941 acattaccat tctgaccgat acggccgtta aagaagtcaa aacatctgac aacgaagcac
    27001 aagtcatttt agaaaacggc aaaatcctga ccgcccgcct gcttttggcg gcagacagcc
    27061 gtttttcgca aacccgccga caactcggca tttcttcaga tatgcacgac tacagccgaa
    27121 ccatgttcgt ctgccgcatg aaacatactc tttccaacca acataccgct tacgaatgct
    27181 tccattacgg gcgcaccatc gcactgctgc cgctggaaga acacctgacc aataccgtca
    27241 ttaccgtcga taccgataaa atcaacagcg ttcaaaacct ttcgcccgaa gaattggcgg
    27301 caagcgtaaa agaacagctc aagggcaggc tcggcgatat ggaattggtc agcagcatcc
    27361 accattaccc ccttgtcggt atgattgcca aacgtttcta cggcaaacgc agcgcgctga
    27421 tcggcgatgc ggctgtcggt atgcaccccg ttaccgcaca cggtttcaac ttgggtctgt
    27481 ccagcgcaga tattttggca aaactgatac tcgaagccga gcagcgcggt caggacatcg
    27541 gcgcggcttc cttgttggaa aaatacagca gcaaacatat gctgcatgcc cagccgcttt
    27601 atcacggaac caatatgatg ctgaaactgt ttaccaatga gaccgcgccg gcaaaactgc
    27661 tgcgcggttt ggtattgcgt gccggcaaca acttcccgcc gctgaaaaaa ctgattacca
    27721 aacaactgac cggttgaaaa ctgccgaaat gccgtctgaa acgtttcaga cggcattttt
    27781 gtattatagt ggattaacaa aaatcaggac aaggcgacga agctgcagac agtacaaata
    27841 gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct atccttggca
    27901 gacggtttgc agcagattcg catacggcag atgtttcaag cggaaaggaa acacgataaa
    27961 aataacaaag cctccgagca gtcggaggct ttgctttcaa actgaaatta tttcagttta
    28021 gtttctttat acactacgtg tttgcgggca actgggtcaa attttttgat ttccaatttg
    28081 ccgggcatag tgcgtttgtt tttggtagtg gtgtagaagt gaccagtacc tgcactggat
    28141 tccagtttga ttttatcgcg cattgcagta atccttaatt aaatagtgtt taaattaagc
    28201 ttcgccgcga gcacgcaaat cagccaatac gacatcaatg cctactttgt cgatggtacg
    28261 cagtgcagcg ttggaaacgc gcaggcgaac ccagcggttt tcactttcta cccaaaaacg
    28321 acgtgattgc aagttgggca aaaaacggcg tttggttttg ttgttggcgt gcgatacgtt
    28381 gttgccggac atcgggcgtt tgccggtcac tttgcaaact cgtgccataa tcagtctcca
    28441 aacttctaaa atagtaaaac gcgatttata ccacgaaaaa cagtgttagt tcaagtgttt
    28501 taaagaaaaa cggctgtttt gcgcggataa tccttccgat tgtcgcatct gtggaacgtt
    28561 tgcaggtgcg aaacagatgg ggcgcgatta tagcggattt cgggaactgc tgcattcaaa
    28621 atgccgtccg aacatatttc cagtattttc agacggcatt tcaagttttc aaatccctca
    28681 atgtccaccg ccgccgccgt tgtggaacgg cggttttgcc agccatatga cgggtatcat
    28741 gataatgaat aacaagctgc ccgccataaa gatttcgttc gagccgataa taaaaccctg
    28801 ctgggtaatg gtattgttga tgatgcctag ggtttgaatg tcggaaacgc cgtgctggga
    28861 caaatgagcg gccgtttcgt gcaatgttgc ggaatagggc gtgatgtgtt cggcaaagcg
    28921 cgtgtggtgc aacgcttcgc gccgttccca cagggtgctg acgacggata cgccgacacc
    28981 gcccatcagc acgcgcagga aattcgacag gctgccggcg gcggcaatct gcccgccctt
    29041 catgtgcgac agtgtgatgg tagtcagcgg caggaagaac atggcaacgc ctactccctg
    29101 ccaaaactgc ggccagatga cgttgccgat atccatgtcg gcataaaaat cggtgcgcca
    29161 atagaaagtg aaggcaaagg tcaggaagct ggcggttacg agcaggcgca tatcgatttt
    29221 attgccgaac ctgccgatta acggagacag gaagacgggc aggatgccga cgggcgcggc
    29281 ggcaagcccc gcccacgtgg aggtatagcc caggttggtc tgcaacacta acggcagcag
    29341 ggtcagcgtc cccatataca ccataaaacc caatgatgtg gcaatgatgc cgacggtaaa
    29401 attccgatct ttaaacagcg ataaatcgac aatcggatat ttttctccca attcccaaac
    29461 aataaaatac gacaagcaca ccagtgcgac tacgcccaag gtaatgattt ctccagaggc
    29521 gaaccagtcg agttccttac ccctgtccaa catcatctgt aacgcgccga taccgactac
    29581 catcaatgta agcccgacat agtcggtcgg cattttaacg gtttccgttt cccgacattt
    29641 caaatgtttc catgtaatcc atgccgatat gataccgata gggatattaa tgaagaaaat
    29701 ccaaccccaa tgccagtttc cggaaatcca gccgccgagt atcggcccga gaacaggggc
    29761 gacaacgacg gtcattgccc acaatgccag tgccagcgtc cgttttgcgg gcggatagga
    29821 tgccattaac aggctttgcg acaagggaat cagcggcccg gcgataaagc cctgcaagat
    29881 gcggaaaaca accagcgact gaaggttggg ggcaataccg cacagccacg atgtgatgac
    29941 gaaaccgaca gcggcggcgg taaacaattt gacctcgccg atacgttttg ccaaaaagcc
    30001 cgttaacggc acggaaacgg cgtttgccac agcaaaagaa gtaatgaccc acgtcccctg
    30061 cgtggttgcc gcaccgaggt tgccggcgat gacgggaacg gcgacattgg cgatagtcgt
    30121 atctaaaact tccataaata cggcaagccc caaagacagc gtaacccacg ccaatgccgc
    30181 acccttaagc ggtggataat ccattgtgtc ctccgtttca gacggcatca gggcgcggat
    30241 tgcccgagga tttcatcgac cgtccgatcg acttccgacc agtcggtact ttccatttcc
    30301 ggcaatgccg cacccggcgt ttttgaaaca ggcgcgcctg cggcggaagt atccacttta
    30361 accgtcatcg acaaaccgat acgcaacgga tgcctgtcca catcttcgcg gttcagcacg
    30421 atacggacgg ggacgcgctg caccacttta atccagttgc ccgttgcgtt ttgcgccgga
    30481 atcagcgaaa acgcgctgcc cgtacctgcc gaaaaacctg ccaccctgcc gcgataaaca
    30541 atttgtttgc cgtacaaatc ggacaccagc tcggcaggct gtccgatttt catatgccgc
    30601 aactgcgtct ctttaaaatt agcatccacc cacacatccg acagcggcac caccgccatc
    30661 agcggcgcgc ctgccgccac ctgctgcccg acctgcaccg aacgcttcgc cacctgaccg
    30721 tccgccggcg cgcggatttg cgtccgctga aggttcaacc acgcatcttt caacctgccg
    30781 attgccgtct gaaccgccgg ctgttcgcgc aaagaaacct gaccgcccaa agccgcacgt
    30841 gccgaagatt cttccgccaa agccgctttg accgccgcct gcgcctgaga cactgccgca
    30901 cgggcgtgtg ccagctcttc ggcggacacc gcgcccgatt ccgccaaagc agaccggcgg
    30961 cgcaaatcat cctgtgcgcg tgccaaatcc gcccggcgca aggcaacctg cgcccccgcc
    31021 tgcgaagtgg cggcattttg ccggcggttt tgccgcaccg cctgaaccag ctcgtttttt
    31081 gcccgctcgt aagccagcac atcattatcg tcgtccaata ccgccagcac gtcgcctttt
    31141 ttcacggcat ccgtatcgtc gtgcaaaacc ttccgcaccg taccgccctt ttgcggcgta
    31201 acctgaacca cgcgtccggc aacataagcg tcttccgttt cctcttcgtg ctgccaccat
    31261 aaaaaaaacg ccgacccggc ggctgcggcg gcaagcgcga acagcagcgt caatgccgtc
    31321 aggcggcgtt tgcgtttggc ttgcgtgttt tgaagttttg tttcgtccgt gtgcgtatcc
    31381 ataagattgg gttccgaatt tcctgatttc atataaacaa tgttctttca ataggaagca
    31441 gattctatat ccgcgcaata acgcaatcaa gtcagtattt tagatgcaat attcaaaaaa
    31501 caacgcttca ggcggcaaaa ctgaaaatat atttcgttta tata
//
LOCUS       DAWFIT010000014        30863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFIT010000014 DAWFIT010000000
VERSION     DAWFIT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.014"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            80..1045
                     /locus_tag="WGQ85_000617"
     CDS             80..1045
                     /locus_tag="WGQ85_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750098.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSIDKNRLPYLDEYFKSTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKKAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1209..2177
                     /locus_tag="WGQ85_000618"
     CDS             1209..2177
                     /locus_tag="WGQ85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0750099.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADRLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2167..3141
                     /locus_tag="WGQ85_000619"
     CDS             2167..3141
                     /locus_tag="WGQ85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0750100.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3186..3812
                     /locus_tag="WGQ85_000620"
     CDS             3186..3812
                     /locus_tag="WGQ85_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750101.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASADELKQFGELWQERVRAFLIDQADNPEFVTVSEI"
     gene            3893..4651
                     /locus_tag="WGQ85_000621"
     CDS             3893..4651
                     /locus_tag="WGQ85_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750102.1"
                     /translation="MITIRNVSHTIGSNPILNDVSLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4710..5048)
                     /locus_tag="WGQ85_000622"
     CDS             complement(4710..5048)
                     /locus_tag="WGQ85_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0750103.1"
                     /translation="MKKIEAVIKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5143..5355
                     /locus_tag="WGQ85_000623"
     CDS             5143..5355
                     /locus_tag="WGQ85_000623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750104.1"
                     /translation="MPETVIPAQAGIPTSAFQQYLKVSVNPNFHIPDRAETTAFDPKT
                     YGSSCPLLCFLQLSAYPALGKNQKNR"
     gene            5513..9475
                     /gene="purL"
                     /locus_tag="WGQ85_000624"
     CDS             5513..9475
                     /gene="purL"
                     /locus_tag="WGQ85_000624"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0750105.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAVQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGNAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFTDPNTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQITQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9570..10322
                     /gene="gloB"
                     /locus_tag="WGQ85_000625"
     CDS             9570..10322
                     /gene="gloB"
                     /locus_tag="WGQ85_000625"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0750106.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            10556..12010
                     /gene="mgtE"
                     /locus_tag="WGQ85_000626"
     CDS             10556..12010
                     /gene="mgtE"
                     /locus_tag="WGQ85_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0750107.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGQ85_000627"
     CDS             complement(12096..13004)
                     /gene="hslO"
                     /locus_tag="WGQ85_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0750108.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13249..14004
                     /locus_tag="WGQ85_000628"
     CDS             13249..14004
                     /locus_tag="WGQ85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0750109.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14023..14241
                     /locus_tag="WGQ85_000629"
     CDS             14023..14241
                     /locus_tag="WGQ85_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750110.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14386..14730
                     /locus_tag="WGQ85_000630"
     CDS             14386..14730
                     /locus_tag="WGQ85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0750111.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            14730..15422
                     /locus_tag="WGQ85_000631"
     CDS             14730..15422
                     /locus_tag="WGQ85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0750112.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            15489..16709
                     /gene="argJ"
                     /locus_tag="WGQ85_000632"
     CDS             15489..16709
                     /gene="argJ"
                     /locus_tag="WGQ85_000632"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0750113.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            16769..18178
                     /locus_tag="WGQ85_000633"
     CDS             16769..18178
                     /locus_tag="WGQ85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0750114.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            18368..19759
                     /gene="hrpA"
                     /locus_tag="WGQ85_000634"
     CDS             18368..19759
                     /gene="hrpA"
                     /locus_tag="WGQ85_000634"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0750115.1"
                     /translation="MSPNFAQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            19860..20987
                     /locus_tag="WGQ85_000635"
     CDS             19860..20987
                     /locus_tag="WGQ85_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750116.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQCPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQGTQVLRIDGTKIVYIK"
     gene            21164..22741
                     /gene="lpt3"
                     /locus_tag="WGQ85_000636"
     CDS             21164..22741
                     /gene="lpt3"
                     /locus_tag="WGQ85_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0750117.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPNKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            22807..26106
                     /locus_tag="WGQ85_000637"
     CDS             22807..26106
                     /locus_tag="WGQ85_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ0750118.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VPYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSAAASAVSNDLHPANPQQTAPSPVGEGREEGKTVASQTNFSATTA
                     NPLPNPLPQEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERE
                     NPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLT
                     VLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVI
                     IPQLAHFIAKSAGDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHEL
                     QQQLGQAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGR
                     IALRLCDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLR
                     DDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELN
                     GKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDA
                     AREADIQELEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSV
                     KRLLKEWEDLN"
     gene            complement(26129..26551)
                     /locus_tag="WGQ85_000638"
     CDS             complement(26129..26551)
                     /locus_tag="WGQ85_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0750119.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(26555..28201)
                     /locus_tag="WGQ85_000639"
     CDS             complement(26555..28201)
                     /locus_tag="WGQ85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0750120.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28215..29270)
                     /locus_tag="WGQ85_000640"
     CDS             complement(28215..29270)
                     /locus_tag="WGQ85_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0750121.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            29560..29772
                     /locus_tag="WGQ85_000641"
     CDS             29560..29772
                     /locus_tag="WGQ85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750122.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            30082..30237
                     /locus_tag="WGQ85_000642"
     CDS             30082..30237
                     /locus_tag="WGQ85_000642"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0750123.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(30327..30776)
                     /locus_tag="WGQ85_000643"
     CDS             complement(30327..30776)
                     /locus_tag="WGQ85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0750124.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7558 a         9000 c         7320 g         6985 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caaaccgaag gcgcgtccct gaccgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccat cgataaaaac cgcctgccct acttggacga
      361 atatttcaaa tccacgaaac ctgccggcac gctgtttgag ccggattacg aaacgctcaa
      421 cgcttacaaa ccgcagctca tcatcatcgg cagccgcgca gccaaagcgt ttgacaaatt
      481 gaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa aaggcggaag ccgacaagct
      601 gaaggcggaa atcgacgcgt cttttgaagc cgcgaaaact gccgcgcaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa aatgtccgcc ttcggtccgt cttcacgctt
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgaa
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtcgtgt acctcgttcc
      961 tgaaacttat ttggcagccg gtggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 cgacgctttt aacgcggcaa aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cctcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac caacctgctg ctgctggcgg
     1261 tgttgtttgc cgtcagcctg tcggtgggcg ttgccgattt ccgctggtct gatgtgtttt
     1321 cactgtccga cagccagcag gtcatgttca tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattttg atgcgtaacc
     1441 gttttgtcga gccttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgttaatcgg gatgttggtg tttatgatgc ttatccgccg cctgccgccg acggcgcaac
     1621 tgatggtgcc tttggtcggg attattttcg gcggcgtggt tgaggcggtg gccaccttta
     1681 ttgcgtatga aaacgaaatg ctgcaaatgc tgggcgtgtg gcaacagggc gatttttccg
     1741 gcgtgttgct cggacggtat gaactgttgt gggcaacggg gattttggct ttgtttgcct
     1801 atttgattgc cgaccggctg acgattttgg gtttgggcga aacggtaagc gtgaacttgg
     1861 ggctgaaccg gacggcgatt ctgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1921 tggtcatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     2041 ctttggtgtt gctgtgcgac attatcggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tctctacggt ttttggtgtg ttgggtacgg ctttgttttt gtggcttttg ttaaggaaac
     2161 ctgctcatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     2221 gttgcctttg cgctgttgct ggtttcctgc atcctgttta tgacgctcaa cgtcaaaggc
     2281 gattgggact ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtttcgac ccagcttttt caaacgctga ccaacaatcc gattctcacg
     2401 ccctcgattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtc
     2521 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2641 ctgctttcgc gcatgatcga tcccgaagaa tttaccgccg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2761 gcggcagcgg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     3001 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     3181 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     3241 aagacccgct tgccgcattc ctcggcgcag cgcaagacgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3361 ttatcaaagg tctgaaagcc ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggaacgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3481 tcctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatgcag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3601 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgcgctccaa ccctttgcac
     3661 cgcaaatgcg cgacatcatg cctaaagccg tcagcggcag cgcaagtgcc gacgaactca
     3721 aacaattcgg cgagctttgg caagaacgcg tccgcgcctt cctgattgac caagccgaca
     3781 atcccgaatt tgtcaccgtc agcgaaatct aaaacgtcat aatcaataag tttcagacgg
     3841 ccctaaaact caaggccgtc tgaaacccca atttccattt tcggacccgc ctatgattac
     3901 catccgcaac gtcagccaca ccatcggcag caaccccatc ctcaacgacg tcagcctcga
     3961 cattcccgaa ggcggcatta ccgcgctgat cggcccgaac ggcgcaggca agtccaccct
     4021 cctctccttt atggcgcgcc tacagcctct ggtacacggc aacatcagct acgcaggcaa
     4081 agacatcaaa accaccccta ccgccgaact cgcccgcacg ctctccatcc tcacccaaga
     4141 aaacagcatc atgagccgca ttaccgtgcg cgacctgctg atgttcggcc gttaccccta
     4201 ccatcaaggc agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt
     4261 ccacctgcaa gacctctccg accgctatct gaccgagctc tccggcggcc aacgccaacg
     4321 cgccatgatt gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct
     4381 gaacaacctc gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgcg
     4441 cgaacacaaa cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc
     4501 cgaccacgtc gtcgccatga aaaacggcaa agccgtcctg accggcacgc ccgaagaagt
     4561 gttcaccgta cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga
     4621 aggcaaaaaa ctcatcgtcc accatatctg aagctcaaaa agaaaatgcc gtctgaaagg
     4681 ctttcagacg gcatttccat tccaaacgct caaaccgccg cgtccgcacg ttcgcccgtg
     4741 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gatttttccc
     4801 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcatcatc cgccaacaca
     4861 agctcgattt tgaccttggg caggaaatcg acggcgtatt ccgcaccgcg atagatttcc
     4921 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatgcc cgtaatgccg
     4981 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgataac agcttcgatt
     5041 tttttcatca cattctccat taattaacgt aaacaaacga caaatatagc agaaaccgcc
     5101 ggcaaacgat aaacggtact gttcgcccgg cgatgcggcg atttgccgga aaccgtcatt
     5161 cccgcgcagg cgggaatccc gacctctgca tttcagcaat atttaaaggt ttctgtaaat
     5221 ccaaacttcc atattcccga ccgtgcggaa acgacggcat ttgatccaaa aacatacgga
     5281 agcagttgcc cgcttctctg cttcctgcaa ttatccgcat acccggcttt gggtaaaaat
     5341 cagaaaaaca gataaaaccc ttattcctta atatcttcag acggcacctg tattttcccc
     5401 aaccgtatgc cgtctgaaga ttgtcaacat tttaagccaa aatatccaag caatacaaac
     5461 ctgtccggcg tataatccct ctttttccca accgcatttg agagccgaat ccatgtctgt
     5521 cgttttgccc ttgcgcggcg ttaccgccct ttccgatttc cgtgtcgaaa aactcttgca
     5581 aaaagccgcc gcactcggtc tgcccgaagt caaattaagc agcgaatttt ggtatttcgt
     5641 cggcagcgag aaagcacttg atgccgcgac tgtcgaaaaa ctgcaagcct tgttggcggt
     5701 gcaaagcgtt gaacaaacgc caaaagcgcg cgagggcttg catttgtttt tggtcacgcc
     5761 ccgtttgggt acgatttcgc cgtgggcttc caaggcgacc aatatcgcgg aaaactgcgg
     5821 tttggcaggc attgagcgta tcgaacgcgg catggcggtg tggctggaag gtgcgcttac
     5881 cgacaggcaa aaacaacaat gggcagccct gttgcacgac cgcatgaccg aaagcgtgtt
     5941 gaccgacatc gatgcggcgg cgcaactgtt ccaccacatc caatcggaaa ctttctccac
     6001 cgttgatgtt ttgggcggcg gtaaagaagc tttggtcaaa gccaataccg aaatgggctt
     6061 ggcactctcc gccgacgaaa tcgattatct ggttgaaaac tatcaggctt tgcagcgcaa
     6121 tccgtccgat gttgaattga tgatgttcgc gcaggcaaac agcgaacact gccgccacaa
     6181 aatcttcaac gccgatttca tcctcaacgg cgaaaaacaa ccgaaatccc tcttcggtat
     6241 gatacgcgac acgcacaacg cgcatcccga aggcacggtc gtcgcttata aagacaactc
     6301 gtccgtaatc gaaggcgcga aaatcgagcg tttctatccg aatgcggcgg aaaaccaagg
     6361 ttaccgtttc cacgaagaag acacgcatat catcatgaaa gtggaaacgc acaaccaccc
     6421 gaccgccatc gcgccgtttg caggcgcggc aacaggcgcg ggcggcgaaa tccgcgacga
     6481 aggcgcgacg ggcaaaggtt cgcgtccgaa agcaggcttg accggcttta ccgtctccaa
     6541 cctcaacatc cccgacctca aacagccgtg ggaacaagac tacggcaagc cggaacacat
     6601 ttcctcgccg ttggacatca tgattgaagg ccccatcggc ggcgcggcgt tcaacaacga
     6661 attcggccgc cccaacctct tgggctactt ccgcactttt gaagagaagt ttgacggtca
     6721 ggttcgcggc taccacaaac cgattatgat tgccggcggc ttgggcagca ttcaggcgca
     6781 gcaaacacac aaagacgaaa tccccgaagg cgcgttgctg atccaactgg gcggcccggg
     6841 tatgcttatc ggcttgggcg gcggcgcggc ttcttcgatg gataccggta caaacgacgc
     6901 ttctttagac ttcaactccg tacaacgcgg caaccccgaa atcgaacgcc gcgcgcaaga
     6961 agtcatcgac cgctgctggc agcttggcga caaaaacccg attatttcca tccacgacgt
     7021 cggcgctggc ggcttgtcca acgccttccc cgaactggtc aacgatgccg gacgcggcgc
     7081 ggtattcaag ctgcgcgaag tgccgttgga agaacacggt ttaacgcctt tgcaaatctg
     7141 gtgcaacgaa tcgcaagagc gttatgtctt gtcgattttg gaaaaagatt tggatgcctt
     7201 ccgcgccatc tgcgaacgcg aacgctgccc gtttgccgtg gtcggcacgg cgaccgacga
     7261 cggtcatttg aaagtgcgcg acgatttgtt ctccaacaat cccgtcgatt tgccgctgaa
     7321 cgtcttgctc ggcaaaccgc ccaaaaccac gcgtaccgac aaaacggtta cgccgtccaa
     7381 aaaaccgttt cacgccgacg atatcgacat caccgaagcc gcctaccgcg ttttgcgcct
     7441 gcctgccgta gccgccaaaa acttcctgat taccatcggc gaccgcagcg tcggcggcat
     7501 gacccaccgc gaccaaatgg tcggcaaata ccaaaccccc gtagccgact gcgccgttac
     7561 catgatgggc ttcaatacct atcgcggcga agcaatgtct atgggcgaaa aaccgaccgt
     7621 tgccctgttt gacgcgcccg cctcgggcag aatgtgcgtc ggcgaagcca tcaccaacat
     7681 cgcggcggtc aacatcggcg acatcggcaa tatcaaactc tccgccaact ggatggcggc
     7741 gtgcggcaac gaaggcgaag acgaaaaact ctaccgcacc gtcgaagcgg tttccaaagc
     7801 ctgtcaggca ttggatttga gcattcccgt gggcaaagac agcctgtcga tgaaaaccgt
     7861 atggcaggat ggcgaagaga aaaaatccgt cgtttcgccg ttaagcctga ttatctccgc
     7921 gttcgcgccg gttcaagacg tacgcaaaac cgttacgccc gaattgaaaa acgtcgagga
     7981 cagcgtattg ctgtttgtcg atttgggctt cggcaaagcg cgtatgggcg gctctgcatt
     8041 cggtcaggta tacaacaaca tgagcggcaa cgcgcccgat ttggacgata caggccgtct
     8101 gaaagccttt tacaacgtga ttcaacagct tgtcgccgaa gacaaactct tggcgtatca
     8161 cgaccgcagc gacggcggtt tgtttgccgt tttggtagaa atggcgtttg cagggcggtg
     8221 cggcttggat atagatttaa atttattgct tgcacaaaca tttattacca accataccgc
     8281 tctgtctcaa tcattgcgga ctgaagaggt aaaagcgttg gctgaatggc aagaaaccat
     8341 tgcccgcaca ttatttaatg aagagttggg tgctgttatc caagttagaa aacaagatgt
     8401 tgccgatatt atcaatttat tctatcaaca acagctgcat cataatgtct ttgaaatcgg
     8461 tacgttaact gatgagaaca cgttaatcat ccgcgacggg caaacgcacc ttatttctga
     8521 caacctaatc aaactgcaac aaacctggca agaaaccagc catcaaatcc aacgcctgcg
     8581 cgacaaccct gcctgcgccg acagcgagtt tgccctgatt ggcgacaacg gacgcagcgc
     8641 attgtttgcc aacctgaaat tcgacgtgaa cgaagacatc gccgcgccgt ttatcaacag
     8701 cggcgcgaaa cccaaaatcg ccatcctgcg cgaacagggc gtaaacgggc aaatcgaaat
     8761 ggccgccgcc ttcacccgtg ccggtttcga tgcctacgac gtgcatatgt ccgacctgat
     8821 ggcaggccgc gtccaccttg ccgacttcaa aatgctggcg gcgtgcggcg gcttcagcta
     8881 cggcgacgta ctcggcgcgg gcgaaggctg ggcgaaatcc atcctgttcc accccgcctt
     8941 acgcgaccag ttcgccgcct tcttcaccga cccgaacacg ctgacattgg gcgtgtgcaa
     9001 cggctgccag atggtcagca acctcgccga aatcatcccc ggcacggcag gctggccgaa
     9061 gttcaagcgc aacctgagcg aacagttcga agcgcgcctg agcatggttc acgtccccaa
     9121 atcagcctcc ctgattctga acgaaatgca aggctccagc ctgcccgtcg tcgtcagcca
     9181 cggcgaaggc cgcgccgact tcgcgcttca cggcggcaac atttctgccg atttgggcat
     9241 tgcgttgcaa tatgtggacg gacaaaacca aattacccaa acctacccgc tcaaccccaa
     9301 cggctcgccg caaggcatcg ccggcgtgac caacgccgac ggccgcgtta ccatcatgat
     9361 gccgcatcct gaacgtgtat accgcgccgc acaaatgagc tggaaaccgg aagactggac
     9421 ggaattgtcc ggctggtacc gcctcttcgc cggcgcacgt aaagccttgg gctaaccgcc
     9481 ctactcaaac caatgccgcc tgaagaatat ttcagacggc gttccggcat accatccttt
     9541 aaacggtatc cgtccaccga ggaacactca tgaaaatcac acccgtcaaa gccttgaccg
     9601 acaactacat ctggatgata cagcacggca accatgccgt ctgcgtcgac ccttccgaac
     9661 cctcgcccgt cttggaattc ctcgtccgca accgcctcat gcttgcccaa acatgggtaa
     9721 cgcaccccca ccccgaccac gagggcggtg cggcggcact ctggcgcggc tacatggaat
     9781 cgcccgttta cggcgaatcc gacatcgaag cagcaaccca caccgtaacc gccggcaccc
     9841 aattcacctt cggcgacgga caggttaccg tttgggcaac acccggccac acagaccgcc
     9901 acaccagcta ccttctcgaa acttcagacg gcatacacgt cttttgcggc gacacccttt
     9961 tttccgccgg ctgcggacgc gtgtttaccg gcacaatcga acagctttac gacagcttcc
    10021 aacgcttcaa ccgcctgcct gaaaacaccc tgttctaccc ggcacacgaa tacaccgccg
    10081 ccaacctgcg tttcgccgcc catatcgagc cggacaacgc cgacattcag acggcattga
    10141 aagcggcaga acgcacgccc accctgcccg ttaccctcgc ccacgaacgc cgcgtcaacc
    10201 cgttcttacg gacagaaatc cccgccgtcc gccaacgtgc cgaagccctg gtcggcaaaa
    10261 cgctgaacag cggtttggaa gtattcgccg ccctgcgcga actgaaaaac gcctactgct
    10321 gacctgccct ccgaaaaatg ccgtctgaaa cccgcgtttc agacggcatt tgcgttaaaa
    10381 atagtaaacc gtttcaaaag ggagtagaat agtgccgttt ccaaccctgc gcctgtaccg
    10441 tcaggctttt attatggacc ttcccagttc gtttttactg aacacccctt ccgattccaa
    10501 acgacaataa ccatcccgcc ggaatgcctc cccgcgcacg gcgggcggag catttatgag
    10561 catcgaacca acccctccga accttgaaaa cgacggtatc gaaaacgatg tagaacgcgt
    10621 ttccgccgat ttcgaccgtg tccactccct ctgcgaaatc ctcgaacctg cttttgaaca
    10681 aatcgaaaac ggcacaccgc tcgaagacgc gccgctgcgc gacaagctga ccgagctgac
    10741 cgtcctcttg ggcgagctgc accctgccga cgtggcggcg gtgttggaat cgctgccgcc
    10801 gcgcgaacgc aacgtcgttt ggcttctggt caaacccgaa gacgacggcg aagtattgct
    10861 ggaagtatcc gacgcggtgc gcgaaacact gatcgagtcg atggataagg acgaattgtt
    10921 ggcggcagtt gacgatttgg acgcggacga attggcggaa ctggcagacg atttgccgca
    10981 ccaagtggtt tacgaagcct tacagacgcg tgatgaggaa gaacgcgccc aagtcaaggc
    11041 ggcaatgtcc tacgaagaca accaagtcgg tgcgattatg gacttcgagt tggtcagcat
    11101 ccgcgccgat gtcgcctgcg aagtggtgct gcgttacctg cgccgcttcg acagcctgcc
    11161 cgaccacacg gataaaatct tcgtggttga cgaaaacgat gtgttgcagg gcgtgctgcc
    11221 catccgcaaa cttttggtcg ccgatcccga agacttggtg gaaaacgtga tggcgaaaga
    11281 tgtcgtgcgt ttccgcgccg aagacgacgt ggaagaagca gcgcaggcgt ttgaacgcta
    11341 tgacttggtt accgcgcccg tcgtcgatga aaacaaaaag ctcatcggca gggtgaccgt
    11401 tgacgaaatg gtggacgtga tccgcgaaga atccgaagca gatatgttga acatggcggg
    11461 tttgcaagaa gaggaagact tattcgcccc cgtcttggat tccgtcaaaa accgctggat
    11521 gtggctcgcc gtcaacctct gcaccgcctt ccttgccagc cgcgtcatcg gcgcgtttga
    11581 aggcagcatc gaaaaaatcg tcgcactcgc cgcgctgatg cccatcgtcg ccggtatcgg
    11641 cggcaactcg ggcaaccaga cgattaccat gattgtccgc gccatggcga tggggcaact
    11701 gacggatatg caggcggggc gtttgctgaa aaaagaagtc ggcgtcgcct tggtcaacgg
    11761 catcatttgg ggaacggtca tgggcgcagt atcttggctg ctttacggca gcctcggcat
    11821 cggtttggtc atggttgccg cgatgacgct caacctgctg ctggcggcaa ccgtcggcgt
    11881 attaattccc gtggtaatgg aaaagttcgg acgcgatccc gcgctgggca gctcggtgct
    11941 gattacggcg gtaacggatt ccggcggctt cctgattttc ttgggtctcg ccaccctgtt
    12001 cctgctttaa atgccgtctg aacccgcgca aaaatgccgt ctgaagcgga agctgcttca
    12061 gacggcattt gactatttat ccttgttgca caagattatt ggacggtatg ccggggcagc
    12121 cctttggcaa cgccgaccac atcctccccg aacagcgcgt tgacatcggt ttcgtcaaac
    12181 acatatttgc tgtggcagaa atcgcaatcg acttcgatgc tgccttgttc caccaccacg
    12241 ccgccgactt cttccccgcc cagcatcagc agcatatcgc tgactttgcc gcgcgaacag
    12301 gtgcatgaaa attcaaacgt ttccggctcg aacacgcgcg gcggcgtttc gtggaacagg
    12361 cggtacaaaa catgttgcgc atccaattct gccagctcgt ccgccgtcag tgtacgcgcc
    12421 agcgtactga cgtgttccca tgcctcttca tccaacaccg cttcaggcag acgctgcacc
    12481 agcagaccgc ccgccgcttc gtcgcttgca gacaggacga tgtgcgtatc gatttgttcg
    12541 gaacgcttca tatagttcgc caacatttgc gcgataccgc cgccttccaa aggcactacg
    12601 ccctgccagg gttcgccgtc tttgggctgc agcgtcagca cgaatacgcc gccctcgccc
    12661 aaaaggtcgc cgaggctttc gtcatcggct atttctgcgg tttcgtccca acgcgcggtt
    12721 gcacggacgg tacggtcgga agccgcttcc gcaaccagca ttttcagccg cccccgcccc
    12781 tgaacctgca caatcagcgt gccttcgttt ttgaggttgc ccgacagcaa cacacccgcc
    12841 gccaacaact cacccaaagc gcggcggatg gcggcgggat agtttttctg ttttacaatg
    12901 tgctgccaca cgttttccag acggacgtgc agcccgcgca cgggcatatc gtcgaagata
    12961 aagcgggtac gcacatcggc gcggttgatg gcggtttgat tcatgatttt ctctgactga
    13021 ttgttcggat ggcggctata tggttgcggt cggcgcgaaa acaagacgga cggcggatgc
    13081 gcttcccaaa ttatcaataa attatataaa aatcaacata ttaactcaat ctaacaagcc
    13141 gttttttgcc aaacagccgt ttttttatat acaatcaaca agatattttc gactgataca
    13201 gcataacatc gcacggcggc acgatgcctc ctgcgcggaa acaccgatat ggattctttt
    13261 ttcaaaccgg cagtttgggc ggttttgtgg ctgatgtttg ccgtccgccc cgcccttgcc
    13321 gacgagttga ccaacctgct cagcagccgc gagcagattc tcagacagtt tgccgaagac
    13381 gaacagcccg ttttacccat caaccgagcc cccgcccggc gggcgggcaa tgccgacgaa
    13441 ctcatcggca gcgcgatggg gcttaacgaa cagcccgttt tacccgtcaa ccgagtcccc
    13501 gcccggcggg cgggcaatgc cgacgaactc atcggcaacg cgatggggct taacgaacag
    13561 cccgttttac ccgtcaaccg agcccccgcc cggcgggcgg gcaatgccga cgaactcatc
    13621 ggcaacgcga tgggactttt gggtattgcc taccgctacg gcggcacatc gatttctacc
    13681 ggttttgact gcagcggctt catgcagcac atcttcaaac gcgccatggg catcaacctg
    13741 ccgcgcacgt cggcagaaca ggcgcggatg ggtacgccgg ttgcccgaag cgaattgcag
    13801 cccggggata tggtgttttt ccgcacgctc ggcggcagcc gcatttccca tgtcggactt
    13861 tatatcggca acaaccgctt catccacgcg ccgcgcacgg ggaaaaatat cgaaatcacc
    13921 agcctgagcc acaaatattg gagcggcaaa tacgcgttcg cccgccgggt caagaaaaac
    13981 gacccgtccc gctttctgaa ctgatttccc aaggaatacg caatgagtat gcccgaaatg
    14041 cccaaatggt acgacgatga cggacagatc gtgtcctgta ccgaaaaggt caaagtgatg
    14101 tccgaaaata tggccgagct gtatcagacg gcacaagacg cgtttgaaga cgcgctgctg
    14161 atgggttgcg gcgaacgtca gttgcgcgat tacctgctcg cgctgattga aggtttggaa
    14221 aatccctacc gcaaagtctg aacacgcccc ggttgctgcg gcacggttta tccgtgccgt
    14281 ttttgcgttt gtgcgcggct tcggcttttc agacggcata tttgacgtta tgattaaaca
    14341 gttaacaaga tttatcacaa cgccgtcaaa agacagacac acaacatgaa catcatccgc
    14401 gcgctcctca tcatcctcgg ctgcctcgcc gccggcgaaa ccgccgtttt cctagcaggc
    14461 atcaaactgc ccggcagcat cgtcggcatg ggcgtgctgt ttgcgctttt gcaggcaggt
    14521 tgggtcaaaa cgtcttggct acaacagctt accgacgcgc tgatggcgaa tctgacgctg
    14581 tttctcgtgc cgccctgcgt ggcggtcatc agctatttgg atttgattgc cgacgattgg
    14641 ttttcgatac tggtttccgc ctccgccagc actttgtgcg tactgctggt tacaggcaag
    14701 gttcaccgct ggatacggag cattatctga tgaacgaaat cctcaggcag cccagcgttc
    14761 tgcttttcct cacgcttgcc gtgtacgcgc ttgcgattat cgtgcgcacg cgcacgggca
    14821 atatcttctg caaccccgta ctcgtcagca ctatcgtgct gattgcctac ctgaaaatcc
    14881 tcgacatcga ttatgcggtg taccacaacg ccgcgcaatt cattgatttt tggctgaaac
    14941 ccgccgtcgt cgtgcttgcc gtgccgctct accaaaaccg ccgtaaaatc ttcaaccagt
    15001 ggctgcccgt catcgtttca cagcttgcgg gcagcgttac gggcattgtt acggggatgt
    15061 attttgccaa atggctgggc gcggaacgcg aagtcgtcct ctcgctcgcg tccaaatctg
    15121 ttaccaaccc catcgccatt gaaatcaccc gctccatcgg cggcattccc gccattaccg
    15181 ccgccaccgt catcattgcc ggtctggtcg gacagattgc cggttacaaa atgctgaaga
    15241 acacagtcgt catgccctcg tccgtgggta tgtcgctcgg cacggcttcg cacgcgatgg
    15301 ggattgccgc ctcgctcgaa cgcagccgcc gcatggcggc atacgcggga ctggggctga
    15361 cgttcaacgg cgtactgacc gcgctgattg cgccgctgct catccccgtt ttgggatttt
    15421 gaacccgttt cagacggcat ttcagcccat gctgtctgaa cgccgacaca ctcgcaagga
    15481 gaaccgttat ggctgtcaac ctgaccgaaa aaaccgccga acaactgccc gacatcgacg
    15541 gcattgccct ctacaccgcc caagcaggcg tgaagaagcc cgggcatacc gacctgacac
    15601 tgattgccgt agccgccggc agcaccgtcg gtgcagtctt cacgaccaac cgtttctgtg
    15661 ccgcgcccgt ccacatcgcc aaatcgcacc ttttcgacga agacggcgtg cgcgccctcg
    15721 tcatcaacac gggcaacgcc aacgcgggta cgggcgcaca gggcagaatc gatgctttgg
    15781 cagtgtgtgc cgccgccgcc cggcaaatcg gctgcaaacc gaaccaggtg ctgcccttct
    15841 ccaccggcgt gattctcgaa ccgctgcccg cagacaaaat catcgccgcc ctgcccaaaa
    15901 tgcagcctgc cttctggaac gaagcggcac gcgccatcat gaccaccgac accgtgccca
    15961 aagccgcctc gcgcgaaggc aaggtcggcg acaaacacac cgtccgcgcc acgggcatcg
    16021 ccaaaggctc gggcatgatt catcccaata tggcgaccat gctcggtttc atcgccaccg
    16081 atgccaaagt ttcccaaccc gtcctccaac tgatgacgca ggaaatcgcc gacgaaacct
    16141 tcaacaccat caccgttgac ggcgacacca gcaccaacga cagcttcgtc atcatcgcca
    16201 ccggcaaaaa cagccaaagc gaaatcgaca acatcgccga cccgcgttac gcccaactca
    16261 aagaattgtt gtgcagcctc gcgctcgaac tcgcccaagc catcgtccgc gacggcgaag
    16321 gtgcgaccaa gttcatcacc gtccgcgtcg aaaacgccaa aacccgcgac gaagcccgcc
    16381 aagccgccta cgccgtggca cgttcgccgc tggtcaaaac cgcctttttc gcctccgacc
    16441 ccaacctcgg caggctgctc gccgccatcg gttatgccgg cgttgccgac ctcgataccg
    16501 acctcgtgga aatgtatctc gacgatattt tggttgccga acacggcgga cgcgccgcaa
    16561 gctacaccga agcacaaggg caggcggtaa tgtcgaaggc cgaaatcacc gtccgcatca
    16621 agctgcatcg cggacaagcc gccgccaccg tctatacctg cgacctgtcg cacggatacg
    16681 tttccatcaa cgccgactac cgttcctgac ccgacacggc ttcagacggc atacataaaa
    16741 ttccgtctga accgccggac aacataccat gacctccaca ttcccccgcc gcctcgcccg
    16801 caaaatccgc caaacccgcc gcctgtcgcg caaaagcatc gcctttctgt tccttttggc
    16861 aggttcggca ctcgtcgccc tgaccgcgct gttttttgcc catcttgccg attttgcgct
    16921 ggaactgaac gccaaactgg ttcaacaata cccatggttc gcgtgggtcg cgcttccttt
    16981 gggtttgccg cttattgcgt ggctcacacg caaattcgcc cccttcaccg ccggcagcgg
    17041 catcccgcag gtcatcgcct cactgtcgct gccctacggc gcacagaaaa cgcggctgat
    17101 ccgcctcggg cagacactgc tgaagattcc gctgaccttt ttgggtatgc tgttcggcgc
    17161 gtccatcgga cgcgaaggtc cgtccgtgca ggtcggcgcg gcagtgatgg gcgcgtgggg
    17221 cgcgtggtgc aagaaacacg gcttggcatt caaagggatg caggaaaacg atttgatggc
    17281 ggcgggcgcg gcgggcggtt tggcagccgc gttcaacgcg ccgctggcgg gcgtgatttt
    17341 cgccattgag gaactcgggc gcggcatcat gttgcgctgg gagaggcaaa ttcttttggg
    17401 cgtgctcgcc tccggtttca tacaggtcgc cattcagggc aacaacccgt atttttccgg
    17461 cttcaacggc ggcgtattgg aacatatctt tctgtgggtc gcactgtccg gcctggtttg
    17521 cggcgcggcg ggcgggctgt tcggacgttt gctctatcgc ggtgcggcgg cgtttgcacc
    17581 gcgcaagata cgcggcttca tccgcaaccg tccgctgctg ctggcggcac tgatggggct
    17641 gctgctcgcc ctgctcggca cgttctacca aggcaaaacc tacggcaccg gctaccacga
    17701 agccgcccaa gccctgcacg gcatctacga agcccccttc ggactcgccg ccgccaaatg
    17761 gctcgccacc gtattcagct attgggcagg cgttccgggc ggcattttca ctccctcgct
    17821 gaccataggc gcggttttgg gcgagcatat cgccgccatc gccgacatat cgcagggtgc
    17881 aaacatcatc gtcctcatct gcatggcggc atttctggcg ggcgcgacac aatccccgat
    17941 tacttccgcc gtcgtcgtca tggaaatgac gggcggacaa agcctgctgt tttggatgct
    18001 gattgcctgc attttcgcct cgcaggtttc gcgccagttt tcgccgcgtc cgttctacca
    18061 cgcatcggga atgcgcttcc gccagcgcgt gcttcaagaa accgccgccc aaaccggcaa
    18121 tgcgcccgca agaccgcaaa cagcaaacag caaaacggga atgccgtctg aaaattaaaa
    18181 cgcccccgat caaacgccgg cagccgcctt gatttgaata ccgttccgcc gccgcttgaa
    18241 atttcagcaa caatgccgtc tgaacgacag aatgcggttt tcagacggca tttccccatc
    18301 ccgatattgc ccgaacccaa ccaaagcgtt tgctataatt tcatttttac cgtacccgca
    18361 cacaaatatg tcccccaatt tcgcccaaac cctctccaaa gaccgccact tcctgcaatc
    18421 cgccttcaaa aatcccaaca aatacggcgg cttgtccaaa gtcgaagaaa aataccgaaa
    18481 atcgcacgaa atctttttga aacgtttggc agccttgccc aaacccgaat tcgacaacac
    18541 cctgcccgtt cacgaaaaac ttgacgaaat caaaaaaacc gttgccgaac atcaggtaac
    18601 gattatttgc ggcgaaaccg gttcgggcaa aaccacgcag ttgcccaaga tttgtttgga
    18661 actcgggcgc ggggcggcag gattgatcgg gcatacccag ccgcgccgtt tggcggcgcg
    18721 ttcggtggca gagcggattg ccgaagagct gaaatccgaa atcggcagcg cggtcggcta
    18781 taaagtacgc ttcaccgacc acacctcgcg cgatgcctgc gtcaagctga tgaccgacgg
    18841 catcctgctg gcggaaacgc agaccgaccg ttatctcgcc gcctacgaca cgattatcat
    18901 cgacgaagcg cacgagcgca gcctgaacat cgacttcctt ttgggctatt tgaaacaact
    18961 cctgccgcgc cgccccgatt tgaaagtcat catcacctcg gcaacgatag acgcagaacg
    19021 cttctcccga cacttcaacg gcgcgcccgt tttagaagtg agcggacgga cgtatcccgt
    19081 cgaaatcctc taccgaccgc tgaccggcaa agacgaagac gacgcagaag tggagttgac
    19141 cgacgcgatt gtcgatgcgg cggacgaatt agcgcgacac ggcgaaggcg atattttggt
    19201 attcctgccg ggcgagcgcg aaatccgcga agccgccgaa gccctgcgca aatccacgct
    19261 gcgccgcaac gacgaaatcc tgcccctgtt cgcacgcctg tcgcacgccg agcagcacaa
    19321 aatcttccac ccctcaggcg cgaaacgccg catcgtattg gcaaccaacg tcgccgaaac
    19381 ctcgcttacc gtgccgggca tcaaatacgt catcgacacc ggcctcgcgc gtgttaaacg
    19441 ctattccgca cgggcgaaag tggagcagct tcatatcgaa aaaatctccc aagccgccgc
    19501 ccgccaacga tccggccgct gcggccgcgt ctccgcaggc gtatgtatcc gattgttttc
    19561 agaagaaaat ttcaacaacc gccccgaatt taccgacccc gaaatcgccc gcagcaacct
    19621 cgccgccgtc atcctgcgca tggcatcgct gaaactcggc gacgtagcgg cattcccatt
    19681 tttagaaatg cccgattcga ggtatattaa tgatgggttt caggtgttgc tggaattagg
    19741 ggcagtggag gccgcctgaa aacaagtagg atatacactt ctacctgaca aagaccaaca
    19801 aacgataaaa ctacggcaca tctagatgcc caacctacaa cattgcacac ggtaaaaaca
    19861 tgaaacactt actcatcgat tttgaaaacg tccagccgca aaacttagac aaattactaa
    19921 ccgaaaatac ccatatttgg ctatttatag gtgtattaca caaaatgtta cctattagtc
    19981 tggtgcaatc cctactacgt ttcggcgaac gtgtccatct tgtccagtta caaaaaacag
    20041 ggaaaaacgc attggatttt tacctgtcct attacctcgg acaaattacc gccacagacc
    20101 ccaatgccca aatcggcata ctctcgcgtg atggaggata cgatgttctg gtcgaacata
    20161 ttttgaaaaa ccaccaggcg aagggtatcg tgcgcctagc caatatagat gaagtacaac
    20221 atcagaaaat tactaccgaa ccgccgtcag cattgctgga aaacactcct cagcctgaaa
    20281 ccaccctcaa accacagcaa ccattaactt cctatttcca agcagcccta actgcactgc
    20341 gccgccccga cgctttccgc ccctgccgcc tgcataacct gcgacaaaat ctgcgtaagc
    20401 atattttgag tgatttgttt aaagaaaaaa ccgatgaaga atgcgaaata accactgcta
    20461 acgttatcaa taaactcaaa gcacaaaact tcatcagcat tgatgaacag gaaaccgttt
    20521 cctaccatct cagtgataat gatttgttac aaagaatcca acgccatatt ttaagccaat
    20581 gtcccaaaac ctacgctgat tttcaagccg tcgtgcaaaa ccgagcagat gcacttcact
    20641 taacagtcgg taccaacgac attcaatcct ttgcgcgaca tttgcgcgac caaaacctga
    20701 tccgccaaaa caatgggaaa attgaatatg caccgtttac tgaacctaaa ccacagccaa
    20761 cacccaagca gcctaaaaaa accgcatggg aacctgatga aattatttgg aaaaaagtga
    20821 ttgccgcgtt atcgttaaag aaccgtccta ataaaaccaa aactttacgc aatacaatcc
    20881 aggcactcac aaaatccaat gcacaagaaa ctgacaaact gctacaacat ttacaaggta
    20941 cccaagtcct acgtattgac ggtactaaaa ttgtgtatat caagtaggat gggcatttat
    21001 gcctgaccta caaaaccaaa aacaacctaa cacccttaat caactccaca aacccttttc
    21061 agacaacctc gttttttgaa aaacaatctg taaacagata actgctgaag aataccgttg
    21121 ccgagcccca aaacctgcct gcaactttta ttgtgaactt cccattatga aaaaatccct
    21181 tttcgttctc tttctctatt catctctact taccgccagc gaaatcgcct atcgctttgt
    21241 attcggaatt gaaaccttac cggctgcaaa aatggcagaa acgtttgcgc tgacatttat
    21301 gattgctgcg ctgtatctgt ttgcgcgtta taaggcttcg cggctgctga ttgcggtgtt
    21361 tttcgcgttc agcattattg ccaacaatgt acattatgcg gtttatcaaa gttggatgac
    21421 gggcatcaat tattggctga tgctgaaaga gattaccgaa gtcggcagtg cgggcgcgtc
    21481 gatgttggat aagttgtggc tgcctgcgtt gtggggcgtg ttggaagtca tgttgttttg
    21541 cagccttgcc aagttccgcc gtaagacgca tttttctgcc gatatactgt ttgccttcct
    21601 aatgctgatg attttcgtgc gttcgttcga cacgaaacaa gagcacggta tttcgcccaa
    21661 accgacatac agccgcatca aagccaatta tttcagcttc ggttattttg tcggacgcgt
    21721 attgccgtat cagttatttg atttaagcaa aatccctgtg ttcaaacagc ctgctccaag
    21781 caaaatcggg cagggcagtg ttcaaaatat cgtcctgatt atgggcgaaa gcgaaagcgc
    21841 ggcgcatttg aaattgtttg gctacgggcg cgaaacttcg ccgtttttaa cccagctgtc
    21901 gcaagccgat tttaagccga ttgtgaaaca aagttattcc gcaggcttta tgacggcagt
    21961 atccctgccc agtttcttta acgtcatacc gcatgccaac ggcttggaac aaatcagcgg
    22021 cggcgatacc aatatgttcc gcctcgccaa agagcagggc tatgagacgt atttttacag
    22081 tgctcaggct gaaaaccaaa tggcaatttt gaacttaatc ggtaagaaat ggatagacca
    22141 tctgattcaa ccgacgcagc ttggctacgg caacggcgac aatatgcccg atgagaagct
    22201 gctgccgctg ttcgacaaaa tcaatttgca gcagggcagg cattttatcg tgttgcacca
    22261 acgcggttcg cacgccccat acggcgcatt gttgcagcct caagataaag tattcggcga
    22321 agccgatatt gtggataagt acgacaacac catccacaaa accgaccaaa tgattcaaac
    22381 agtattcgag cagctgcaaa agcagcctga cggcaactgg ctgtttgcct atacctccga
    22441 tcatggccag tatgttcgcc aagatatcta caatcaaggc acggtgcagc ccgacagcta
    22501 tattgtgccg ctggtgttgt acagcccgaa taaggccgtg caacaggctg ccaaccaggc
    22561 ttttgcacct tgcgagattg ccttccatca gcagctttca acgttcctga ttcacacgtt
    22621 gggctacgat atgccggttt caggttgtcg cgaaggctcg gtaacgggca acctgattac
    22681 gggtgatgca ggcagcttga gcattcgcga tggcaaggcg gaatatgttt atccgcaatg
    22741 agtggcgtaa aaaccaataa agacaaattt agatgatgtc ggggaagatg cccgaccgac
    22801 aagactatgc aaaatatgaa aaaccaagta cgcggatcag gcatggatgc ccgatccaac
    22861 ccggccaatg tttcagacgg cctgcaaaac agttcgggtc atatcggtac caacacgcgt
    22921 taccgcctga ccaaactcgg cgaacaaatg gcgcgcctgc ccatcgaccc gaaaatcgcg
    22981 cgcattttgt tggcggcgaa gaagcacgac tgcatggcgg aaatattggt gatcgcgtcc
    23041 gcgctgtcga ttcaagaccc gcgcgagcgg ccgctggaag cgcgtgatgc cgcggccaag
    23101 gcgcacgagc gttttaccga caagcaatct gactttcttg cctatctgaa tatttgggac
    23161 agctttcagc gcgagcgcga caaaggtttg tccaacaagc agctggtgca gtggtgccgc
    23221 caatattttc tgtcgcacct gcgtatgtgc gaatggcgcg agctgcacca ccagcttgcc
    23281 caaaccgcga ttgaaatggg tttgaccacc aaggaaaccg ctttcagacg acctcccgaa
    23341 atcaggcagc tcacgtcgtc tgaaaatgcg ggtgaccaag acttatccgc caagctcaaa
    23401 caaaaacaac tggataaaaa gcaacaccgc gcccaaatcc gcgccgccaa agaagcgggc
    23461 tacgaacaaa tccaccgcgc cctgctgacc ggccttatcg ccaacgtcgg catgaaatcg
    23521 cccgacggca acgactacac cggcgcgcgc ggcagccgct tccacctttt ccccgcctcc
    23581 gccctgttca aagccaaacc caaatgggtg atggcggcag aactcgttga aacgacaaaa
    23641 ctttatgcgc gcgacgtcgc cgttatccag cccgaatgga ttgagcagga agccccgcac
    23701 ctcgtccgct accactattt cgagccgcac tgggaacaaa aacgcggcga agtcgtcgcc
    23761 agcgaacgcg tgacgcttta cggcctgacc gtattgccgc gccgccccgt gccttacggc
    23821 aaagttgcgc ccgaagaagc acgtgaaatc tttatccgca gcgcgttggt ggcgcaggaa
    23881 tgcgatttga aagcggattt ttttgtccac aataaaaagc tgattaaaga aattaccgaa
    23941 ctcgaacaca aatcgcgcaa gcaagacgta ttggtcgacg acgaagccct gtttgcgttt
    24001 tataacgaac gcctgcccga aatggcttgg aaagacgcgc aaggcagcgt ttggggaagt
    24061 gaagattccg tacggattat tgaatctgac aaagccgaga ggtcgtctga aaatgagcgc
    24121 aacgagtttc gtaaaaacaa gcgtaatggg tctcgccaaa atgaaaatca cggcaacacc
    24181 gtaggttggg ttgaaaaccc aacatcagcc gcaactgcaa aaactgttgg gtttgacaac
    24241 ccaacctacg ctactcagca acccaccccc tcccccgcga gggagggtcg gggagagggc
    24301 aaaacagttg ccgcacaaac caacttttcc gcaaccgcag caaaccctct cccgcaggag
    24361 agggagcaga gtgccgcagc ttcagcggtt tcaaacgacc tgcatcctgc aaatccccaa
    24421 caaaccgccc cctcccccgt gggggagggt cgggaagagg gcaaaacagt tgcctctcaa
    24481 accaacttct ccgcaaccac agcaaaccct ctccctaacc ctctcccaca ggagggggaa
    24541 cagagtgccg cagcttcagc ggtttcaaac gaccctcaac cccaaaagca gccagcaccc
    24601 caaaaagacc gtctgaaacc cctccccctc gccgacatcc gcaccttcca agcctggctc
    24661 aaaaccgccg aacgcgagaa cccgcgcctg ctgttcctca gccgcgacga tttgatgcaa
    24721 cacgccgccg cacacattac cgaagagcaa ttccccaaat tctggcaaac cgcagacggc
    24781 aagttcaaac tttcctaccg cttcgagccg caccatccgc tcgacggcgt taccctcacc
    24841 ctaccgctga ccgtcctcaa ccgcctgcac gcgccgtcgc tcgaatggct ggtgcccggt
    24901 atgttgcgcg aaaaaatcca gctgctgatt aaagcactgc ccaagcaaat ccgccgcatt
    24961 tgcgtgcccg tacccgattt catcaccaaa tttctcgaaa gcaatcccga ccgccaagcg
    25021 gtcattattc cccagttggc gcactttatc gccaaaagcg caggcgatat gcggattttc
    25081 gagcaaatcg accaagacgc atgggcggcg caagaattgc ccgaacactg ctatctgaat
    25141 ctgctcatca tcgacgacgg cggacaagag cttgccggcg gccgcaaact gcacgaattg
    25201 caacaacagc tcggccaagc cgccgccgtc accttccgcg acaacaccca agaattcgag
    25261 cgcgacaacg ttaccacatg ggatatcggt accctgcccg aatccatcaa attcgcccgc
    25321 ggcaaacaac agctcaccgg ctacctcggt ctgcaaaaag aaaaagacgg ccgcatcgcc
    25381 ctgcgcctgt gtgacaccac cgaagctgcc gaacaggcac accgtcaagg cgtcatcgaa
    25441 ttgatgaagc tgcaattaaa agagcaggta aaagacctga acaaaggcat ccaaggcttt
    25501 acccaagccg ccatgctgct caaacacatc aacgccgaca ccctgcgcga cgacctcacc
    25561 caagccgttt gtgaccgcgc ctttatcggc gaagacgagc tgccgcgcaa cgaaaaagcc
    25621 ttcaaagaac aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agccctcagc
    25681 cgctacctgc aggaaaccgc cgccgcctac gccgaactga acggcaaact cggcaaacac
    25741 ccattgaccc accttctaag actacgcctg caaaccctgc tcgccgccgg cttcgccacc
    25801 cgcaccccgt gggcacaatg gccgcgcctc cccatctacc tcaaagccat gaccctgcgc
    25861 ctcgaaaaat acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatacaggaa
    25921 ttggaacaaa tgtggcagga aaaaaacgac ggtttggtga aacaaggact acccgtttca
    25981 gacgacctca ccgcgtttaa atggatgatc gaagaattga gggtgtcgct gttcgcgcag
    26041 gaattgaaga caccgtatcc ggtgtcggtg aagcggctgt tgaaagagtg ggaggatctt
    26101 aattaaagga agatgccgtc taattttttc agacggcatc ttttatttcc ctgaccaacc
    26161 gatttaatcg ctccctacaa atagctttat tttttaattc gcactcccaa acgacaatca
    26221 ctttgaaacc gatcttttcc agctcggttt catttttaat atcacgttcg caattttttg
    26281 tgattttttc caaccaaaaa tccgtattgc ttttcgggat atgtccttta ttacaagaat
    26341 gcccgtacca aaaacagccg tgtataaaca cgactgtttt atatttcgac aaaacgatat
    26401 ccggtttgcc tacatagcgt ttatcgtttt tccgataacg gaaaccttgg aaaaaaagaa
    26461 atttccttac caatacttcc ggtttggttt ctttgctatg gatttttgac atcacgaaag
    26521 aacgtttgga tgtagtgaaa atatcggtca taaattactt ggaagatacc accttcgatc
    26581 gtttttttgt ctgttccact tttcttaaca aatacgaaac caagcttatt aaatccaaag
    26641 catccttatc attaaaaata ctaggagata aatttgctct taactcatgt tgtacaggat
    26701 ttctaaatcc agtccatgtt ccgcatgcaa gctgttcaag accatcttct aaattttgtt
    26761 ccgccttcgt tttgttattc gttaatagaa gtatagaatt tttactcccg aaagcttgtt
    26821 caatcaattc tcttccgtct atgctatgaa gacctgtttg cttctgaacc ttatcttcga
    26881 aaactttaac tgcctcgatt gcagcactta aataatcctt cttttcattg taaaaatcat
    26941 tacaggcagt atgcaaatcc tgatgcaaat gccgccaatg atagtaagga tattctggaa
    27001 taatactatg taaggcagag attacttccg cttgctcttt atttgtaagc tccgaatctt
    27061 ccaaacgatt caaaataact tctactggtt ccctttcctt ttctggcaat ttgtttttcc
    27121 aatcttcgat atcaactccg ctttcgcctt tcacttcgtc atctttctct ttttttcttc
    27181 tttttctcca atcttgcccg atggctcgga cgatctcccc aaggaaaaca cgtaattttg
    27241 aggtttttga atgcttccag tctaatgatg tcctgtcggt agcaattaaa tcttcatcgt
    27301 cattatctat aaagtccaca ttcagccatc cagtcaaata tgaatagaaa tggctggatt
    27361 cgctatcagt gaaaaactcg ggcatattta ccattctgcc gttggcaaac aaggtaatac
    27421 ctttcatatt attctttaaa ggtttttccg ttgtaatgaa cttaccttgt attaatccgc
    27481 tgtattcata ctgttcgaat tttgaagata acgatgaaat attggttgct aaatcctggt
    27541 attcccattc gaattgtggg gtaacaagat catattttag atttccatca atgattttag
    27601 gttcagagcc gttcaagctt actttgattt taaaatcctg agccggaaaa tcaaacaagc
    27661 gggaaagatg ctctacataa ttatctaacg gatatccttg ctttttcgtc agttcggtta
    27721 aagttatggt tgtcccgctt tcgatattgg attcaacaga ttcttttcga aactccggtt
    27781 gataaatacc cttgcttctt cgaatctctg cataatccaa agtaaaagta accctttcgt
    27841 ttccctggat agtagaaatt tcaattttgt tgccaagccc gaataatgcc aatttaccaa
    27901 ggcctttttt acccgttgga attcttccgc acggggaggc ttgtttttct tcccttctgt
    27961 tccgaccgat tctcaaataa aaatcattga tttcatcgaa gctcattcct ataccattat
    28021 ctttaataac gattttatgc tccggtttat cgaataacct aacttccact tccgtagcac
    28081 atgcatcgta ggcattcgct atcaactcgg caatcgcagg aacagtgtgc gaatacatct
    28141 ttacacccaa atgttcgatt acggttggct cgaaactcat tttcaatttt tcttcagaca
    28201 tgttttctcc ttttctaaca ttcgatatta tcaacaattg ccctgcctat tttttccgca
    28261 tacatcggag gaacggcatt accaatcaaa cgggctatct tgtccctgct gcccgcttta
    28321 aagacatagt tacgagggaa cgattgcaat gttgcacctt cgcgcagtga taacgcccta
    28381 tcttcttcag gatgggcaaa cctcccgttg gaaatactaa agaatttggt cgttatggta
    28441 ggtgcgggct tatcccacca tagcctacca aacgtgtctt taaaactatt gtcttttccg
    28501 ataaaacatt ccagctgtaa ttcgggaata tgcgcaaaag ccaaacggtt tcctccgttt
    28561 ttaggtatca aagccaatcg tttcaagttg atatcagata atcccgcaca gctatgcata
    28621 aaatccgttt cgtcttggtg tcctgcaata attttgggaa agccattttc cattcccaaa
    28681 acatcgcgta ccgtaagccg tttgcccgaa tacttgactg gttccagctt ttctttggtt
    28741 attctgtttg caattaacgt aaatctttta cggctttggg gaattccgta atcagccgta
    28801 ttgtgaatac cgaaatggac ggtaaatcct ttttcttcca ataactttat gaaattatca
    28861 agcctgcttt ctttcattct gctcagaatt cccggtacgt tctctacaac aacatatccg
    28921 ggattgaaat attctacaaa ccgctgaaac tccaaaagca aactcttgga tttttccgat
    28981 tttcttttgt ctgtttgaat aacactccaa tattgacaag gactacaacc tataagaatc
    29041 aaatcatcat cattcttttt taacccgagt tctttttcca aggtttcagg ctgcaattca
    29101 aaaacatcag cctgtataaa tttggcattt atattggctt catatgtttc tttgcagctg
    29161 ggttcgtaat ctatcccggc taaaacctga ataccggcgg attgcattcc atagctcatt
    29221 ccacctccgc cacagaaaaa atcaaccgcc tttaacttac tgtttttcat ttctatttaa
    29281 ataattagac tagtgagaac taactttata taacatataa tttattgctt atcaaaaata
    29341 atttgagatt ttattaaaat tcatgcatct aaataagcag aacttaattt cctccaaacg
    29401 agcggatgtc ttcaaatgca cacttcacag aaaggtcgtc tgaaacccat tttcagacga
    29461 cctttttcca cttcccaaaa caagaattcc caccccctcc ccaaacgtcg cccgaaccag
    29521 taaactcccc atatttcaac aacttcttgg aagccaacca tgccccgtat ccaccctcct
    29581 cacctgttcc cgccccatat cgccgaacgc ggccttttgt attttcagca gggcaaggtt
    29641 cgcgatgtcc gaaaaacttc cgccgggcgt tatcgggcgg aggtatgcgg tttggaaaac
    29701 tattgggtat ggctgaagct ggatagtgat ttgtatatta aagacgaaag ctgcgattgc
    29761 ccttatgtct aaaggtgcaa acatacctta aattactata ttacataggc aaaatacaag
    29821 cctataacga attggaaaca aaatgccgtc tgaaaacatc ttcagacggc attataaaat
    29881 ctgttcacct tttcagatga gtaatgtaca cccctataca atttttgcta ctatacccca
    29941 taaatccacg gctaaagata tccttattat gtcctatgat ttatcgaaac gacttgtaat
    30001 cggcttagca tcaagtgccc tattcgactt atccgaatcg gataatatat ttagaatgga
    30061 aggggcagaa acctataggc aatatcagag agaaaaacaa aaccatcccc taaaaaaggc
    30121 gttgtctttc catttattaa aaaacttctg tcaatcaatg aaataaaccc aaacgaccca
    30181 acgattgggt ttattctttt atccagaaac aatccagata cagattacga gtcataacta
    30241 taggcttaat attacacgat tttcattcca tcaaggcgga aaaccgcaca aatactgaaa
    30301 cactatcgat cgatttgtaa acaagcctac ttaagtaact tgcagtcctt atcatttcct
    30361 ttaaaataat ccagcccgtc actacacgaa ctggcggact tcttgcaaat aaaggttact
    30421 agattttcat tcatcttaat aataaaagga tttttatctt tatctatggc taccgccttc
    30481 aacatgaatt tactgtctaa ggccccgcgc gcgattccat tcaaacggat acaaaagcct
    30541 tctgcctctt taatcggcaa acttggccat ttggtagatg tttgtttaaa tctcccattc
    30601 tgcagataaa acttttccat aaaatgtgca ttttctaaca aggctgcccg cactgtattt
    30661 atctttgctt tctcaacata attgcgatag ctcggataaa caattaaagc aagtacagac
    30721 aatatcaaga ccactgatat taattcaacc agcgtaaacc cccgattatc agtcattact
    30781 ttacttccaa taagaacaga ttattcaaca tatttctttg aacagactta ctatcccatt
    30841 cagcagtatg catatttccc act
//
LOCUS       DAWFIT010000015        30441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFIT010000015 DAWFIT010000000
VERSION     DAWFIT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.015"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGQ85_000644"
     CDS             complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGQ85_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0750125.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2317..2589)
                     /locus_tag="WGQ85_000645"
     CDS             complement(2317..2589)
                     /locus_tag="WGQ85_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750126.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2775..3461
                     /locus_tag="WGQ85_000646"
     CDS             2775..3461
                     /locus_tag="WGQ85_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750127.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3593..3931
                     /gene="erpA"
                     /locus_tag="WGQ85_000647"
     CDS             3593..3931
                     /gene="erpA"
                     /locus_tag="WGQ85_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0750128.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3948..4094
                     /locus_tag="WGQ85_000648"
     CDS             3948..4094
                     /locus_tag="WGQ85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750129.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4091..4555
                     /locus_tag="WGQ85_000649"
     CDS             4091..4555
                     /locus_tag="WGQ85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0750130.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPELPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4590..6101
                     /gene="ubiB"
                     /locus_tag="WGQ85_000650"
     CDS             4590..6101
                     /gene="ubiB"
                     /locus_tag="WGQ85_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0750131.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGQ85_000651"
     CDS             complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGQ85_000651"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0750132.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7460..8038
                     /gene="grpE"
                     /locus_tag="WGQ85_000652"
     CDS             7460..8038
                     /gene="grpE"
                     /locus_tag="WGQ85_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0750133.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8062..8202)
                     /locus_tag="WGQ85_000653"
     CDS             complement(8062..8202)
                     /locus_tag="WGQ85_000653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750134.1"
                     /translation="MKKNRQAAYGLTVNAETKCRLKPDRRVQTALPLYFDRFIRKTRF
                     RK"
     gene            complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGQ85_000654"
     CDS             complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGQ85_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0750135.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8403..9458)
                     /locus_tag="WGQ85_000655"
     CDS             complement(8403..9458)
                     /locus_tag="WGQ85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0750136.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVHLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGQ85_000656"
     CDS             complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGQ85_000656"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0750137.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGQ85_000657"
     CDS             complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGQ85_000657"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0750138.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(11439..12065)
                     /locus_tag="WGQ85_000658"
     CDS             complement(11439..12065)
                     /locus_tag="WGQ85_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0750139.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(12065..12841)
                     /locus_tag="WGQ85_000659"
     CDS             complement(12065..12841)
                     /locus_tag="WGQ85_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0750140.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(12834..14066)
                     /locus_tag="WGQ85_000660"
     CDS             complement(12834..14066)
                     /locus_tag="WGQ85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0750141.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(14069..15412)
                     /locus_tag="WGQ85_000661"
     CDS             complement(14069..15412)
                     /locus_tag="WGQ85_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0750142.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(15760..17181)
                     /locus_tag="WGQ85_000662"
     CDS             complement(15760..17181)
                     /locus_tag="WGQ85_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0750143.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(17539..17898)
                     /locus_tag="WGQ85_000663"
     CDS             complement(17539..17898)
                     /locus_tag="WGQ85_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0750144.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(17895..18500)
                     /locus_tag="WGQ85_000664"
     CDS             complement(17895..18500)
                     /locus_tag="WGQ85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0750145.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(18547..19029)
                     /locus_tag="WGQ85_000665"
     CDS             complement(18547..19029)
                     /locus_tag="WGQ85_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0750146.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      19233..19330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19541..20641
                     /gene="gcvT"
                     /locus_tag="WGQ85_000666"
     CDS             19541..20641
                     /gene="gcvT"
                     /locus_tag="WGQ85_000666"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0750147.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            20804..21190
                     /gene="gcvH"
                     /locus_tag="WGQ85_000667"
     CDS             20804..21190
                     /gene="gcvH"
                     /locus_tag="WGQ85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0750148.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            21342..22589
                     /locus_tag="WGQ85_000668"
     CDS             21342..22589
                     /locus_tag="WGQ85_000668"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0750149.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            22865..23290
                     /locus_tag="WGQ85_000669"
                     /pseudo
     CDS             22865..23290
                     /locus_tag="WGQ85_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            23296..24526
                     /locus_tag="WGQ85_000670"
                     /pseudo
     CDS             23296..24526
                     /locus_tag="WGQ85_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            24584..25204
                     /locus_tag="WGQ85_000671"
     CDS             24584..25204
                     /locus_tag="WGQ85_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750150.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            25215..25346
                     /locus_tag="WGQ85_000672"
                     /pseudo
     CDS             25215..25346
                     /locus_tag="WGQ85_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            25343..25837
                     /locus_tag="WGQ85_000673"
     CDS             25343..25837
                     /locus_tag="WGQ85_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0750151.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(25971..27632)
                     /locus_tag="WGQ85_000674"
     CDS             complement(25971..27632)
                     /locus_tag="WGQ85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0750152.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(27762..28305)
                     /locus_tag="WGQ85_000675"
                     /pseudo
     CDS             complement(27762..28305)
                     /locus_tag="WGQ85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(29239..29892)
                     /locus_tag="WGQ85_000676"
     CDS             complement(29239..29892)
                     /locus_tag="WGQ85_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0750153.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSGFIHYRINHSKECADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT         7325 a         7407 c         8149 g         7560 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatataa aaaatttcca gagaaccgat acaacagttg
       61 gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat gctatggtct
      121 tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag acggcatcaa
      181 ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat cgtctttctt
      241 tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg cttgagcgta
      301 aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg catcgatggc
      361 ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt cgattttttc
      421 tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca cggagtgaat
      481 cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat cttcctcagc
      541 attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac ctgaagaacc
      601 ttggatggta atgttggctg ctttaccggt gcctttgtct ttggcggaaa cgtgcaggat
      661 gccgttggcg tcgatgtcga aggttacttc gatttgcggc ataccgcgcg gtgcaggtgc
      721 gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt cgcgttcgcc
      781 ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga acacttgcga
      841 cgctttagtc gggatagttg tgttcttttg aatcagcttg gtcatcacgc cgcccatggt
      901 ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc ggccgccgct
      961 taacacttcg ccttgaatcg ctgcgcctac ggcgacagct tcgtcagggt tcacgtcttt
     1021 gcgcggctct ttgccgaaga agtctttaac ggcttcttgt actttcggca tacggctttg
     1081 accaccgacc aaaatcacgt cgtcgatgtc gccagtgctc agaccggcat ctttcaatgc
     1141 ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt cgaatttggc
     1201 gcgggtaatt ttcatcgcca agtgtttcgg gccggttgcg tccatggtga tgtacggcag
     1261 gttaatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg cagcttcttt
     1321 caggcgttgt agagccatca cgtcttgttt caaatcaatg ccttgttctt ttttgaactc
     1381 ggcgatgatg tggtcgatga ggcgttggtc gaagtcttca ccgcccaaga aggtatcgcc
     1441 gttggttgcc aatacttcga attgtttgtc gccgtcgagg ttggcgattt cgatgatgga
     1501 aatatcaaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt tgttgtcgcc
     1561 tttgtccata ccgaatgcca aagcagctgc agtcggctcg ttgatgatgc gtttcacgtc
     1621 caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt tgaagtaggc
     1681 agggacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg cggcttcttt
     1741 cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc cttgtgcttt
     1801 tacccatgcg tcgccgttgt tggctttgat gatttcgaaa ggcatagatt cgatgtcgcg
     1861 ttggacttct ttgtcttcaa atttgtggcc gatcaggcgt ttggcggcgt aaatggtgtt
     1921 tttagcgttg gttacggctt ggcgtttggc aggcgcaccg acgaggattt cgccgccgtc
     1981 caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga tcactttggt
     2041 ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga taccgattac
     2101 ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg ttggaacatt
     2161 ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac agcatggcta
     2221 taagtatata actttatgaa tatattggtt ttatatttga tttaatacat ttggctccaa
     2281 tgcatttaag cataatattt caaatggcag gcaggtttat tcatagacga tgccggcgag
     2341 catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag actcgatgat
     2401 gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt tcagtatgtt
     2461 gaatttttcg cacagggatg cggataaagt gatgtcaggc tgtttggctt cagaacggtt
     2521 ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat atttgccagt
     2581 tttcttcatt ttctatcctt tttctgtcaa ctcaggatta aacctatgga aaatcttaaa
     2641 aattatgtat tgagtaagaa aaatcataat ttaaatttag tttatcatag ttattccgtt
     2701 tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga atgaagggga
     2761 gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg aaaagcgaag
     2821 cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc agcaggatat
     2881 ggaatgaagg cggtctgccg aagtctgaaa cattaaaaaa aatcaagcag ttgaaggggt
     2941 gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa gccccaaaaa
     3001 aatcccttgc ttacgatacc ttgggaaatg aagtcgatac ggacgagttt gtctttgtgc
     3061 cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat gaggaaccgg
     3121 tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc gatacgaaaa
     3181 acctgtctgt aatttccgtc aagggggatt cgatggaggg ggttttgaat gacggcgatt
     3241 cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg ttgcggatta
     3301 atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc aatgtgattt
     3361 ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc gatgatgtgg
     3421 agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc ttgaaattgc
     3481 aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc tgcttgcctt
     3541 tgaagggggt ttgagagaga atgcagaaaa tattatatta aggaataaca ccatgtcgga
     3601 tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt tgattgccga
     3661 agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct gttcgggttt
     3721 ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa ttgagaaaaa
     3781 cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg cggaaatcga
     3841 ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg cggaaacaac
     3901 ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg ccgtctgaac
     3961 gttcagacgg catttttact tttagaaaat atattatcgg gatgaattca catataatcc
     4021 gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta tcaggcgtat
     4081 ttgttccctt atgattgaga tgagtaaaga ttaccgaaac gatttgtacg atgtatatgt
     4141 ttcttacccg ccccaagtgg atcgcgggct tatccgggag tgccttaagg agaatctcgg
     4201 cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag tgctggttga
     4261 ggaaaaatgc acttgggcga aacgggaaga gttgcatgat tatttcagct atttgggttt
     4321 ggatattatt acccggagat atatggagtt agaaacggtc gtgccgccgg aggaaggtga
     4381 gggcgaagga gagggcgcgg atggggaaat gcccgaatat cttgaacttc atggcgggcg
     4441 ggaagatgat atttccgcac cttcgcaacc cgaactgccg acccgcaata tcaaaccgct
     4501 gcttttcggg ctgctgattg cctttttggg ctatctgctc ggtaagattt tttgattgtc
     4561 cgataaatgc tgtattcggg attttatata tgaaatggtt gaaacgcctg acggtcattg
     4621 tcgggacttt ttaccgctat cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt
     4681 ggatatgcgc tctgctgaaa atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg
     4741 ccgtccgtct gcgccttgcc ttggaaagcc tggggccgat tttcatcaag ttcgggcagg
     4801 ttttgtccac acgccccgat ttgattccgc acgattacgc cgtcgaactg gcaaagctgc
     4861 aagacaaagt gccgcctttt gacgcgcggc tttcgcgtga acaaatcgaa aaatcgttgg
     4921 gtcagtccat cgaaaagctg tatgcggaat ttgaaaccga gcccatcgcc agcgcgtcca
     4981 tcgcccaagt acacaaagcc cgcctgcatt cgggcgaacg agtggcggtt aaagttttgc
     5041 gccccaacct tttgcccctt atcgaacagg atttgtcgct gatgcgtttc ggcgcggcct
     5101 gggtcgaacg cttgttttca gacggcaggc gtctgaagcc gctcgaagtg gtggcggagt
     5161 tcgacaaata cctgcacgac gagttggatt tgatgcgcga agccgccaat gccggacagc
     5221 tcgggcgcaa tttccacaac agcaatatgc tgattgtgcc caaggtgttt tacgactact
     5281 gcaccagcga cgtgctgacg attgaatgga tggacggtac gccggttgcc gacatcgcca
     5341 aactcaaagc agacggcatc gatttgcaca aactcgccga ttacggcgtg gaaatctttt
     5401 tcacgcaggt cttccgcgac ggctttttcc acgcggatat gcaccccggc aatattttgg
     5461 ttgccgccga caaccgctac atcgccctcg atttcggcat cgtcggcacg ctgaccgatt
     5521 acgacaaacg ttatctcgcc atcaatttcc tcgccttttt caaccgcgat taccggcgcg
     5581 tcgccaccgc ccacatcgaa tcgggctggg tgcctgccga cacgcgtgcg gaagaattgg
     5641 aagccgccgt ccgcgccgtg tgcgaaccgg tgttcaacaa accgatttcg cagatttcct
     5701 tcggcttggt gctgatgcgc ctgtttgaag tcagccgccg cttcaatgtc gaaatccaac
     5761 cgcagcttgt gctgctgcaa aaaacgctgc tcaacatcga aggcttgggc cgccagctcg
     5821 atcccgattt ggacttgtgg aaaaccgcca aaccgttttt ggtgaaatgg atgaacgggc
     5881 aggtcggccc taaagccctt tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa
     5941 tcattccttc attgccgcgc aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa
     6001 tgcgtgatgc ctatatccat ttggtcaaag tgcagcagcg gcaaagcctg tggctggctg
     6061 tgattgcggt tgttttgctg ctgattttgc ttttgaaata ggctttgtcc gaatcatcgc
     6121 ccgactccgc ccgtttataa ggaaatcggt tatagtggat taacaaaaac cagtacggcg
     6181 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     6241 attccgtact atccgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     6301 ctatatttcc ggttgcgtgg gaatcgggtg tatcgaataa aaggcatttt gtccgactgg
     6361 caagtgccga catcggcggc atatcaaggc gcaggcttga agcgggcaat gtcgtctgaa
     6421 gcccgtttgg cgtttcagac ggcattggtg cggatattca aatcataaag tcgatttcgg
     6481 taaactggat attttgatcc atatccgccg acggtgtttt gagcgatcgc gccacgggtt
     6541 tggcgggtac gccgacaacc gtgatggacg gcggcacgtc tgaaaccacg acgctgcccg
     6601 ccccgatttt ggcattgctg ccgatgcgga tattgcccaa tatcgaggcg tttgcgccga
     6661 tcatcacgcc gtcgccgatt ttagggtggc ggtcgccgcc ttctttgccc gaaccgccga
     6721 gcgttacgcc gtgcaaaatc gaaatattgt tgcccaacac ggcggtttcg ccggcaacaa
     6781 agccggtggc gtggtcgagc atcagcccgt atccgaaacg ggcggcggga tggatgtcca
     6841 cgccgaatac ttcggacata cggttttgca ggaaatacgc cagcgttttg cgcccgtcga
     6901 gatacagccg atggttgatg cggtgtgcct gaatcgcgtg gaagcctttg aaatataaaa
     6961 gcggcagcga atattcgtcg caggcgggat cgcgttcgta gatggctttt aagtctgctt
     7021 cgacgcattt gccgatttgg gtgtcgctgc ccaacgcctg ctggtagatt tcaaacagcg
     7081 cgcgcacgtc cataatcggg ctgccgagtt tgctggaaag gtggtaggca aggacggagc
     7141 cgagggactc gtggcgcaac acggtttggt gcaaaaaact tgccagcatc ggttcggcgg
     7201 agaccgcggc cgcggtttct tcgcggatgg tgtgccagag gtcgaaaccg gttgtgttta
     7261 aatggtcttt tttcatgagt gatgacgttt gaaaatcgat atggtcggca gtatcttacc
     7321 gtctatatta ttttttcggt aggggatttg aaaatgaatt tgaaattctc tgcctttgct
     7381 tgaagtttct tgaaaatgtc cttatcttgc gcgggtaata actggatttt gatttccaat
     7441 ttgttttaag ggatacgata tgagcgaaca gacacagcag caaaacagtg aagaagcggt
     7501 tgaaaatgtg gaggcggtgg aaaccgtcga gacagtagga aatgcggacg gtgtgcagga
     7561 acaggctgcc gcagagccgg cttatgagga tttgcaggcg cggattgccg agctggaagc
     7621 gcagttgaaa gacgagcagc tgcgcgcttt ggcaaacgag caaaacctgc gccgccgcca
     7681 ccagcaggaa attgcggata cgcacaagtt cgccggacag aagtttgccg tggaaatgct
     7741 gccggtcaag gattatctgg aaatggcgct tttggatcag agcggcaatt tcgatgcgct
     7801 gaaaatgggc gtgcagatga ctttgaacga gttgcagaaa gcatttgatg ctacgcaaat
     7861 caaggaaatc aatcctaaag cgggcgataa gctcgatccg aatatccatc aggcgatgca
     7921 ggcggtggca agcgaacagg aacccaatac cgtggtgggc gtgatgaaga agggttatac
     7981 gctgtccgac cgcgtgttgc gcccggctat ggttacggtg gcacagaagg aagcctgaag
     8041 gcgtctgggg aataatctga tttatttcct gaagcgcgtt ttgcgtataa accgatcgaa
     8101 gtaaagcggc aatgccgtct gaacccgcct gtcgggcttc agacggcatt ttgtttcggc
     8161 gttaacggtc aacccgtatg ccgcctgcct gtttttcttc atccagtttc ttttgcaggg
     8221 tgtcgcaagg tgtgtcgcag tcgcacattt ttttcatacc caaggcagta atgccgccgc
     8281 aactgccttt gatgctgcgt ttggagaaaa tatagccgac cgccataccg atgatgacgg
     8341 tcaggaagat gccgaaggta aggagcaggg ttttcatggt gtttcctaat cggtttgtat
     8401 gtttagcgga gcagtttttc aaattcggaa gacatggcgg tgcggtagcc gcctttatcc
     8461 ctgacaatca ggaaaacagc gagtttttcg cgctctgcca gctttaaggc ttcggtttcg
     8521 cccaatacga ataatcctgt ggacaagccg tccgccgtca tcgcactgtc tgcgaccacg
     8581 ctgatggagg cgaggttgtg gctgatgggt cgtttgttgt tcggattaat gatatgggag
     8641 aggcgtttgc cgcttttatc gacgtggaaa atacggtaat cgccggaagt ggcaagcgaa
     8701 cggttgttca gcgggacgat aatctgcgta ttgccgcctt ggacgatgtt gggctgttcg
     8761 atgccgatgc gccaaggttc gccgcgcgcg tttttgcctt tgccgtgcaa ctcgccgccg
     8821 atttcgacca gataattttg aatgccgtat ttttccagtt cgcccgcaac tttatcaacg
     8881 ccgaagcctt tggcaatcga agataaatcc aaataggcct tggggtgggt tttgctcaag
     8941 gaagcgtaat ctttgccttg tttcaaaatg attttgtcta tgcccgtata agatgctgct
     9001 tgtttgattt gttccggcga cggttcacgg gtaacggatt tgtcggggcc gaatccccaa
     9061 aggttgacca aggggccgac ggttacgtcc agcgcgccgt gtgtcaggcg gttcaggtgg
     9121 acggcttcgg cagtaacgtg tgcgaagtcg cttgaaatgc ggaggggctt gccggctgtg
     9181 tgttggttga accggctgat ttcggagtcg ggctgatagg tggacatctg ccggttgact
     9241 tctttaagcg cgtcatcgat gcgcttttgt atttcggcag gtgaggggag tttgtcccga
     9301 ttatttgaaa ggtatttgac ggtataggtc gtgcccatcg tttcaccttg cagggtaacg
     9361 gtttgcgcgg tttgttccga acaggcgttc aggaagataa aactcagggc aaatatcaag
     9421 gtgcggataa agttcggcag gcgtgtttca gacggcatag tgtttgacgg ttttggcaaa
     9481 tggtttgaat tatatcgcaa aacggccggt atgtttctat gccgatgccg tctgaagggt
     9541 gttcggatgg catcggcata gaaaaaggaa gaaaccgagg tttcttcctt ttgtatttga
     9601 agccgaatac tcaaccgccg aaatcatcca agaggatgtt ttcgtcttcc acgcccaagt
     9661 ctttgagcat tttgatcacg gactggttca tgattggcgg accgcacata taaaattcgc
     9721 agtcttccgg tgcttcgtgg tttttcagat ggttttcgta aaccacgttg tggatgaagc
     9781 ccgtgtagcc gtcccagttg tcttccggca gcgggtcgga cagggcgacg tgccacgtga
     9841 agttcgggaa ttctgctgcg agttggtcga agtcttcgac atagaacatc tcgcgtttgg
     9901 aacgtgcgcc gtaccaaaag gtaatcttac gtttggagtt caaacgtttc aactggtcga
     9961 aaatgtggga acgcatcgga gccatacccg caccgccgcc gataaatacc atttcggcat
    10021 cggtgtcttt ggcgaaaaat tcgccgaacg ggccggaaat cgtaactttg tcgccgggtt
    10081 tgagcgacca gatgtaggac gacatttgtc ccggaggcgc atcaggtacg cgcgggggcg
    10141 gcgtggcgat acgcacgttc agcataatga tgcctttttc ttcaggatac gaagccatag
    10201 agtaggcacg caaaatcggc tcgtccactt tggaaacgta ttgccacaaa ttgtatttgt
    10261 cccagtcttc gtggtattcc tcgggaatgt cgaagtcttt gtaggcaacg gtgtgcggcg
    10321 gggcttcaat ttgaatgtag ccgccggcgc ggaaggggac ttcttcgcct tcgggaatgg
    10381 caagcttgag ttctttaatg aacgtggctt tgttatcgtt ggagatgacg gtgcattccc
    10441 attttttcac gccgaacact tcttcgggga cttcgatgtc catgtcggtt ttgacgttga
    10501 cttggcacga cagacggcag ccttcgcgtg cttcgcgttt gctgatgtgg gacagctcgg
    10561 tcggcaggat gtcgccgccg ccgcttttta cgacgacgcg gcattgtccg cacgaaccgc
    10621 ccccgccgca ggcggagggg ataaagatgc cttcgttggc aagcgcgccc aagagtttgc
    10681 cgccggcggg catcgtcagc tctttttcgc cgttgacttt gatggtgatg tcgccttcgc
    10741 tgaccagttt ggatttggca aacagaatca tcagtgccaa aaccaaaacg atgacggtaa
    10801 acatcacgat acctaaaata atctccatac cgatcccttt cttataactg gatgccagag
    10861 aacgacataa acgccatcgc catcaggccg gcggcgataa aggtaatgcc caagcctttg
    10921 aggcctttgg gagcgtccga atatttcatt ttttcggtaa tgcccgccaa agcgacaatc
    10981 gccaacatcc agcccaagcc cgcaccgaag ccgtacacga cggattcgcc gaagccgtat
    11041 tcgcgctgcg ccataaacga tacggcaccg aaaatcgcgc agtttacggt aatcagcggc
    11101 aggtagatgc ccagcgcgtt atagagggcg gggacgaatt tatccaagaa catttccaaa
    11161 atctgcacca aagcggcaat cacgccgatg aaggtgatga atttcaaaaa ggtcaaatcc
    11221 acgccttcga caatcgcgcc gtctttgagc agcgagtaaa ccaattggtt ggcggggacg
    11281 gacagtccga gtacgaaaat caccgccaca cccaaaccga atgcggtgga tacttttttg
    11341 gataccgcca aaaacgtaca catacccaaa aagaaggata gcgccatatt ttcgatgaag
    11401 acggatttga taaagaggct caaatagtgt tccatagctt attcctccgc ctgttcgggt
    11461 ttccaggtac gcagtcccca aatcaaaaag ccgatgatga agaacgcgct gggggcgagc
    11521 aggaacaagc cgttggtctg ataccagccg ccgtcctgca cggtttggaa aacggtgtag
    11581 cccaagagtt tgcccgagcc aatcagttcg cggacggtgg cgacgacaag cagcatcatc
    11641 ccgtagcccg cgccgttgcc gatgccgtcg atcaggcttt ccagcggcgg ctctttcatc
    11701 gcaaatgctt cggcgcggcc catcacgata cagttggtaa taatcagacc gacgaatacg
    11761 gaaagctgtt tggacaattc gtaggcaaat gcctgcaaga gttggtcgac cagcgtaacc
    11821 agcgacgcga taatcgccat ttgcacgatg atgcggatgc tgttggggat gtagttgcgt
    11881 accagcgaaa tgaagaagct ggaaaaaccg gttaccaaag ctacggaaat acccatcacg
    11941 atggccgtct gaagtttggt ggtaaccgcc aaagccgaac aaatacccaa aacctgcaag
    12001 gcaatcgggt tgttgtcgat aaagggtgaa aacatcaaat gtttcaagcg tttcatatca
    12061 gccattattg cgctcctgct gatttcaatt tgttcaggta ggggatatag ccgttttcgc
    12121 cgaaccagta ggcgaacgaa ccttgtacgc ctttggatgt cagcgacgcg ccggagaggg
    12181 catctacgcc gtgttctttg tccgaacccg cgcctttgcc gacgtgcagg gcgagtttgc
    12241 cttgtccgtc aaacagtttt ttgccgacga atttttgctg ccacaaagga ttgccgattt
    12301 cgccgcctaa gcccggggtt tcgccttgct cgtagtaggt aatgccgttg atggtgttgc
    12361 cgtcgggttg gatggcgaca aagccgtaca tgaccgacca caaaccgtta ccgtgcatag
    12421 gcaggatgat ttggccgatt ttgccgtctt caccttttac caagtaaacc tcggtgtatt
    12481 tggcacggct tttgatgcct gccaaatcgt cttcgggttt gatgcggatg ctttgcgcgg
    12541 ggtctttgcc tgcgatgcgc gcactgaagt ctttcggcgc gtctttcaca tattcgccgg
    12601 tcgccaaatc gaccacgcgt tgctcgatac gctcggcaaa agttttaccg atgtcggtgt
    12661 ccttatccat caaaccggct acgctcaaga tatagccttg tttgtcttgg agtttttgtt
    12721 tctcttggat gggtttcaag ccgacgaccg caccggcaac gatgaccgag caaatcaggc
    12781 tgaccgccaa cacgacaatc agcgtgccgc tgaagctgtc tttatcgaat ttcttagcca
    12841 ttgctgcgcg cctttctgcg tttgatgttc gcttgtgcga cgaaatagtc gaaaatcggg
    12901 gcaaacaggt tggcaaacag aatcgccaac atcatgcctt cggggtaagc cggattgacc
    12961 acgcggatta atacgcacat cacaccgatc agtgcgccgt accaccattt gccgacattg
    13021 gtaaaggaag cggaaacggg gtcggtcgcc ataaacagca taccgatggc gaagccgccg
    13081 acgaccaaat gccagtacca aggcatagca aacatagcgt tggtgtccga accgatgaag
    13141 ttgaacagcg aagacatggc aatcataccg atcatcacgc cggcaataat gcgccaagaa
    13201 gcgatgcggg caaacacgat aaacgcgccg ccgattaaga gtgccaaagt ggagacttcg
    13261 ccgatggagc cgggcagttt gccgataaac gcatccatcc aagtgatggt ttgaccggtt
    13321 acggcgtttt tcaggccgtc tgcaccgtgt gccgcccatt gcgccagcgc ggttgcgccg
    13381 gaatagccgt caaccgccgt ccaaaccgca tcgccgctca agttggcagg gtaggcgaag
    13441 aacaggaagg cacggcctgc cagcgcaggg ttcatgaagt ttttacctgt accgccgaat
    13501 acttctttcg caaccacaac gccgaaagaa atacccaaag ctgcctgcca cagcggcagc
    13561 gtgggcggaa cgattaaggc aaacagaatc gaggtaacaa agaaaccttc gttgatttca
    13621 tgtttgcgca cggtggcgaa caaaacttcc cagaaaccgc cgacaataaa tacggtcgcg
    13681 taaatcggca ggaagtaaat cgcgccgaac agcattttgc ccaacacgcc cgcttcagac
    13741 gacatattga tgcccaaagc gttggcaagg gcgtaatgcc agtcgttggc gatgctttgt
    13801 tgcagcaaat cgggcgttaa cgcaccgaat gcctgtgcgc cgacgttgta cataccgtag
    13861 aacatggcag ggaacaaagc cagccacacc aaaatcatca tgcgtttgga gtcgagcgca
    13921 tcgcggacgt gtgccgcttt gcgcgttacc gcaccggatg tatagaaaat cgtcgccgca
    13981 gcttcgtaga gggcatacca tttttcatgt ttgccgcccg gcaggaagtg cggttcgatt
    14041 ttttccagaa aatgtttcaa gcccataatc agccttcctt ctcaatggtt tccagcacct
    14101 tacgcaacag cgggccgtat tcgtatttgc ccgggcagac gaagctgcac aaagcgaggt
    14161 cttcttcgtc caattccaag caacccaatg cttgcgcgct gtcggtatcg ccgacgatta
    14221 aatcgcgcaa aagcagggta ggcaggatgt ctagcggcat tacgcgctcg taagtaccaa
    14281 tcggcaccat ggcgcggtcg ccaccgttga cggctgtcgt gaacttgaag agtttgtttt
    14341 tcaggaaatg gccgagggtc gtacgcgtga tggagtattt gtccggctgc ggcgcaaccc
    14401 agccgaacag ctctttgctg cggccttctt cgataacgga aatctgattg tggtagcgtc
    14461 ccaaataatc gtgcgcgcct tgtgtaatcg cgccgttcaa taccgaaccg gaaatcacgc
    14521 ggttgtctgc gtcaaccaat tcgcccgcag taatttgcga tactttcgca cccaaaacgg
    14581 tacgcaagag gcgtggtttg ttgacttgag aaccacccaa agcaatcacg cgctcggtgt
    14641 tcagacggcc tgttgcaaac aaacgtccga tggcaattac atcttgataa ttgatggtcc
    14701 aaacggtttt gtttgcaccg accggctcaa tgaaatgaat gtgcgtgcca ctcaaaccgg
    14761 ccggatgcgg gccgccgaat tcatgtgttt cgatgttggc agcattttca gacggcacgt
    14821 ctgcgccagc tgccttacac acatggattt tacgctcggt caaacggctc aataccagca
    14881 aacctcgtct gaaatcctcg gcggcttctt tgatcacaac cacagggtct gccgccagcg
    14941 gattggtgtc catcgcattg acgaagatgg cgaacggctc ggcatcgacg gcagggattt
    15001 tgctgaacgg acgggtacgc agcgcagtcc acaaaccgga ttggatcaga ttgcggcgca
    15061 cttcttcgcc gcttaagttt gccaacgctt cgggcgcgta gcgttcgaac tcgatttcgt
    15121 cgttgccttc aacggcaatc acgaccgact gaagtacgcg cttttcgccg cgatggatgg
    15181 cggcgatttt gcctgaaacc ggcgcggtaa acaccacgcc cggattcttt ttgtcttcaa
    15241 acagcacttg gccttttttg acggcatcgc cttccttgac tttcatcgag gggcgcatac
    15301 cggcatattc ttcgccaagc aacgcgactt cggtaatgac gggcccgtca taaatgactt
    15361 gctccggtct gcccgcgatg ggcaggttta gacctttttt gattttaatc atatatttgc
    15421 attacttgtg atggttaagg taaaaacggc gtgttttgat accgtgtcgc gtggcatcaa
    15481 aagcattgaa taaattaatg tagcaaagtg ttagattcta tcaggaattg tacctgtttg
    15541 tcagatttgc tgcttttttc cttgcggaag ctgtttttat agtggattaa atttaaacca
    15601 gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
    15661 ctgatttttg ttaatccact ataattgtcg gaagggggga tattgatttg attatgccgg
    15721 aatttaaaat gccgtctgaa tgttcagacg gcatagcgtt tacagcagtt tgaaaacgaa
    15781 aaagataagg gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc acatcatata
    15841 gccgaagaaa gtcggcatcg gtacgccgcg ctgttcggca atggccttga ccatgaagtt
    15901 cggtgcgttg ccgatgtagg tcagtgcgcc catgaatacc gaacccatag aaaccgccag
    15961 cagcgaatga aacagggaac ccgtcatcaa ggcttgggca tcgccgcccg ccatattgaa
    16021 aaaaacgaga taagtgggcg cgttatccaa gaatgccgac aatatgccgc tcatccaaaa
    16081 atacatcaca ttaatcggat gacctgccgt atcgtgaacc agcgatacca ccccgcccag
    16141 cgcgcctgcc tcgcctgctt tcagaatgct caggacggga aagatggtga tgaagatgcc
    16201 gaggaagagt ttgcccactt cggcgatggg ttcaaagttg aattcgttgc ctgcgcggac
    16261 ttgtttgggc gtgattgcca tagatacggc ggtcaatgca atcaggatga catcgcggac
    16321 gaggttttgc agggcgtaac ggctgccgag gatttcaaat cccgggtgtt cgggtttcca
    16381 aaggccggac attagaaccg cgccgaccac gcccgaaagc aggaggaagt tccatttgcc
    16441 gaagatggcg attttttcgg gtttttcctg ttgtgccggc gtatcttgtg caatgctttc
    16501 ctgtttgaag aaacggttgt cgatgaaata gaaggcggtc aacaggacag cggtgctgat
    16561 caggacgggg gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt tgaggaagcc
    16621 gaggaagagt ggggggtcgc ccaaaggggt cagaccgccg ccgatgtttg caaccaggaa
    16681 aatgaagaag atgacgatgt gcacgcggcg ggtacggttt tggttggctt tcagcagcgg
    16741 acgaatcatc agcattgctg cgccggtcgt tcccatgata gaggcaagtg ccgtaccgac
    16801 ggcaagcagg gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa ccaaaatgcc
    16861 gcctgaaatg gtgtacaggg caagcagcag caggatgaaa gggatgtatt cttcaacgag
    16921 tgcgtgtgcg acggtatgga taccggcgga cgcgccaaaa accaaactga acgggatgag
    16981 gaagagcaat gtccaaaagg cggtaatttt gccgtaatgg tgatgccagg tatgcgaaaa
    17041 aaacaaggga cccaatgcga tagacagcaa aatcagggca aagggcaggc tccacagcag
    17101 gtttaggttt gcgccgtcca aatctgcggc gtaaaccgat gcggggaaaa gcattagtga
    17161 aaacaggggt aggtggcgca tcgtgtttcc tcgattcaag cactgccttg cgcggcgcgt
    17221 gggagtgata caggcaccgt gccgcccgga cataggcgga ctgtgctgcc tgtttgtttt
    17281 tgaaaaaagt tttccgattg attgtaaagt aaataatcag gataaaccag tttcccaaac
    17341 cggaaggcgg cgggaaggcg gattgctgtg cttgggaata tatgttcttt ttgataaata
    17401 attttattta aaacaaataa atatatgtct ttaataaata atctatcgaa aacgaaaaat
    17461 gaatttattt taacatatat ttgcaatgaa acaggtttgc cccccgtttg tttgccctta
    17521 tccctttcag tacggcattc aagattcggg cctgcgccac atccatatgg cgacaaggga
    17581 acaaaaaacc gatgaaaccg ccccgaccca ccagcgttgg ggaaactgcc aaaacattat
    17641 caggcaggat gcggtcatca tactgatggc gaatattttg gctttgcgcg gcactgcgcc
    17701 gttttgttcc cagttatgaa ccatcggacc gaaatagcgg tgccggtgca gccagcggtg
    17761 aaagcgcggg gatgcctttg cccagcaggc ggcggagagc agtacgaacg gcgtggtcgg
    17821 caacagcggc aaaaaaatgc cgatgatacc caacagcagg gaaatgcagc cgcaggcaat
    17881 taaaagataa cgtatcattt tgaaatattt ttcttattgt gcggataagg gcaggatgtg
    17941 ataccgagtt ttgcccagcc ttcatgtccc attttttcca gcagggcgat attgcgttcg
    18001 aatattgccg aagtgtcggg aaaggcttgt gcggctttgg caatgctgtc ttcgcggatg
    18061 aggtgcagcg tcggataggg agaacggttg gtgtagttgc cgatgtcgtc gggttcggta
    18121 ttctgaaagc ggaattgggg atggaacggt gcgatttgga cgatgccttc caagccgttt
    18181 tcgataacgg cggcatcggc aatatcgagc atatcgttga atacgtcgaa atcggggaat
    18241 agggtcgggt gaaccagcag ggtggtttcc agttcggtgg cgggtgtatt gcccagtcgc
    18301 tgcagttctt cgtccaagtc ttccaaaaaa ctgtcaaggt gtttggcttc gctgattgcg
    18361 atgcggacaa ggtttttaac gtggggggct ttggcaaagg gacacaggtt cagaccgatg
    18421 acggcttttt ccaaccattg tccggtgtgt tcggcaacag catctttatt ttcggaagta
    18481 ttgatattca ttattgtcat gtaaatgtgt ttgcagattg cacgtgcggg aaaatcggga
    18541 agggcactat tccttcagca ggtggttgag cggcagggag gtggtgtgtt tgatttcttt
    18601 taaaacaaag ctcgattgcg catcttgtac gccgtggtgg gacaggagcg tatccaaaac
    18661 aaaatgggaa aacgcgttca tatcggtaaa aaacgcctga agcaggtagt cggtttcccc
    18721 tgtcagggcg aagcagctca agacttcagg ccattttcgg accgatgcgg caaagtcttc
    18781 ccgcgcgtct tttgctttgc ggatggaaac gcggataaat gcctgaagcc ccaggttgac
    18841 ggattccgga gacagcagcg cggcatattg gcggacgatg ccggcatctt ccaactgctt
    18901 tagacggcgc aggcacggag aaggcgaaag tgcgacacgt tcggacagtt cgacattggt
    18961 cagcctgccg ttttcctgga gaacctgtaa gattttaata tcggttttgt ctaaagtgag
    19021 ttggggcata tttgcgttcc gttttaagga attcggattg tctgtccgta tgtttgcggc
    19081 aatccgcaca gatggagacc atattaacat ataaaaagtt ataccgtcat ccgggacaaa
    19141 ttttgttttc ggaaaatcat gtgaaaacag aggcggtcgg tttgcatctc ttttaagacg
    19201 gcttgcccaa accgccgatt caagacataa tcgggaaatg tgcaggagag tgttacaccc
    19261 aactacaatg taaccaccga aggcgcagac acccttaaat cgctcaggta tcagggactg
    19321 cacattgaaa caaacaatct ggagagcggc gttggaataa cgtccaccga aggggagaag
    19381 gccgtctgaa ccaccattca gacaaccgcg caaagcagtg agcagactgg tttgccatca
    19441 tgcggatacg gccgaaaatc tcaggttcaa ggacagatag ggtcatccgc gcacaggtgc
    19501 gcgggtggca tctgaacaaa aaatccggag aaacttgaga atgattactc tgaaaaccac
    19561 cccatttcat caagcccatc aagatgcagg cgcgaagccg gtagattttg ccggctggga
    19621 gctgcccatc cattatggtt cgcaaatcgc cgaacacgaa gccgtgcgca ccgacgccgg
    19681 tatgtttgac gtatcccata tgcttgttac cgacgtagca ggcgcaaatg ccaaagcctt
    19741 tttccgcaaa ttgattgcca acgatgtcgc caagctcgct tttgtcggca aagcccttta
    19801 ttccgctttg ctcaacgaca acggcggcgt gattgacgac ttgatcgttt accgcaccaa
    19861 tgaagccgaa acccaatacc gcatcgtgtc caacggcgcg actcgcgaaa aagatacggc
    19921 acaattccac aaagtcggac aagagttcgg tgtttccttc aatccgcgct acgacctcgg
    19981 catgctcgcc gtacaaggcc ctaaagccat tgaaaaactc ctgaccgtca aacccgaatg
    20041 ggcagatgtc gtccataacc tcaaaccgtt ccaaggcgcg gatttgggca acgactggtt
    20101 tgtcgcccgc accggctaca ccggcgaaga cggcgtcgaa gtcatcctgc ccggcaccga
    20161 agccgtcgca ttcttcaaag ccctgcaagc agcaggcgta caaccttgcg gtctcggcgc
    20221 gcgcgacacc ctgcgcatgg aagccggcat gaacctctac ggcaacgata tggacgacga
    20281 taccagcccg ctcgaagcag gcatgggttg gaccgttgat ttgaaagacg aaagccgcga
    20341 ttttgtcggc aaagctgcct tgttggcatt gaaagaaaaa ggcgttgccg tcaaacaggt
    20401 cggtttgctg ctcggcaaag gcggcatcct gcgcgcgcat atggaagtgt tgaccgacaa
    20461 aggcaaaggc gaaaccacca gcggcgtatt ctctccaagt ctgaaacaat ccatcgccat
    20521 cgcccgcgta ccgaaagatt ttgacggcga taccgccaaa gtgctgattc gcggcaagga
    20581 agcggacgtg cgcgtactga agctgccgtt cgtccgcaac ggtcaaaaac agtttgatta
    20641 atatcggttc agacggcatt ttcatttcat atgccgtctg aaagcaggtt ttaattgtcg
    20701 tccgatacgg acgtttgtag aaagcattga acaaggcatc tgtggatatt gattcatgca
    20761 gatgccgtct gaaaataacc ccctatcaat ggagtatcaa accatgagca gcaacatccc
    20821 ggcagaactg aaatacgttg ccagccacga atggctgcgc cttgaagaag acggtatcat
    20881 caccgtcggt attacccacc acgcgcaaga gctgttgggc gacatcgtgt ttgtcgagct
    20941 gcctgaagtc ggcgcaaacc ttgccgccga agaacaagcc ggtgtggttg agtctgtaaa
    21001 agccgcgtcc gacgtgtacg caccgattgc aggtgaagtc gttgccgtca acgaagattt
    21061 gccaagcgct ccggaaactg ccaacagcga tccttacggt gcaggctggt tcttcaaact
    21121 caaaccggca aacgttgccg attacgacgt tctgctgact gccgaacaat acgcgggcga
    21181 agtggattaa accgcccggc tgcccgatgg caaccgccgg acaaacggaa actgcacctt
    21241 cagacggcat ttttgcggtc ggaggtgcag ttttttgtcc gtgttttaag gaagcagtta
    21301 ggctataata acggtctata ttcatcttta ccgatttttt catgcaactt accgctgtcg
    21361 gactcaatca tcaaaccgca cctttaagca tacgggaaaa gctggcgttt gccgcggcct
    21421 gcctgcccga agccgtccgc aatcttgccc gaagcaatgc ggcaacggag gcggtaatcc
    21481 tttctacctg caaccgtacc gagctttact gtgtaggtga ttcggaagaa atcatccgtt
    21541 ggctcgcaga ctatcacagc cttcccatag aagaaatcag cccctacctt tatactttgg
    21601 ggatgcagga gactgtgcgc catgctttcc gcgtcgcctg cggcttggat tcgatggtgt
    21661 tgggcgagcc gcagatttta ggacagatta aggatgcggt cagggttgct caagagcagg
    21721 aaagtatggg taagaaactc aatgccctgt tccaaaaaac cttttctgtt gctaaagagg
    21781 tccgtaccga tactgccgtc ggcgaaaact cggtttccat ggcttccgct tccgtcaagt
    21841 tggcagagca gattttcccc gacatcggcg atttgaatgt cttgtttatc ggtgcgggtg
    21901 agatgattga gctggttgcc acttattttg ccgccaaaag tccccggctg atgacggttg
    21961 ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa gctcggtgtc aacgccgaac
    22021 cgtgcctgct gtccgatctg cctgccattt tgcatgagta cgacgtggtg gtttcttcaa
    22081 cggcaagcca gttgcccatt gtcggcaaag gtatggtgga gcgcgcattg aaacaaaggc
    22141 agagtatgcc gttgtttatg cttgacttgg ccgtgccgcg agacattgag gcggaagtcg
    22201 gagatttgaa cgatgcctat ctttatacgg tggacgatat ggtcaatatc gtccaaagcg
    22261 gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac gctggtgtcc gagaaggttg
    22321 ccgaatttgt caggcagcag cagggcaggc agagtgtccc gttaatcagg gcattgaggg
    22381 atgagggaga gaaagcgcgc aaacaggtct tggaaaatgc gatgaaacag cttgccaaag
    22441 gcgcaacggc agaagaggtt ttggaaaggc tgtcgatcca actgaccaac aagctgctgc
    22501 attcgccgac ccaaaccttg aataaggcgg gggaagaaga taaagatttg gttcacgccg
    22561 tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg aaaaggatgc cgtctgaagg
    22621 ggtttcagac ggcatttttt gtgcaggccg gcaacatcgt gaaatcccac attatatcga
    22681 tgtaatcaca aagtatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    22741 gtacagatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat
    22801 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
    22861 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
    22921 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacagtgt tttttaccgc
    22981 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
    23041 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggcaa
    23101 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
    23161 cgatgcggtc aatacgcgcg gttattcgaa caaaccgatc gatacgctga tggctttggc
    23221 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
    23281 cggtatcccg catttgcccg cgcccatgcg ggcgatacgc tcaaactggc ttccggcgta
    23341 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc tgaccgttct
    23401 gcaaccatcg aaggagacag gagcggacgt acgatagctg tgcatgcgcc ggacgtaacg
    23461 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ctgcaatgga tgccggtatt
    23521 tatctcgaag aaaccgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
    23581 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gcgagaataa aattgtcggc
    23641 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcacccggc
    23701 gcgcaggtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc
    23761 acgcacaaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
    23821 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat aggctatgtg
    23881 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
    23941 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggca
    24001 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
    24061 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
    24121 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
    24181 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
    24241 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
    24301 gtattgcgcc tcttgatgaa cagtctcgca atcagcatcg tcaaatgggc gcaggcgcag
    24361 tttcccgccg ttctgcccgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
    24421 ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ttgctcaaag aagccgaaac
    24481 gcggcagtcg gaatggggca gggcggaaaa cggttctttg aactagtctg tttcagacgg
    24541 catccggatt caaatgccgt ctgaaaacac aaaaggaaaa accatgacca cacatcatgt
    24601 cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcaacc aagtcgattt
    24661 ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg gcaagtccac
    24721 cattatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga tgcttttggg
    24781 cgaacgtacc ggcagcaaag cgggggcgcg gcttcgcagc caaatcggct acctgcccga
    24841 aaccgttgcg ctgcaccctt cgctgaccgg catcgaaacg ctggattttt atgccaaact
    24901 taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca tttcacaggc
    24961 ggcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg ccttggcaca
    25021 agccctgctg ggcgagccca aagtcctgct gtttgacgaa ccgacaaccg gcctcgaccc
    25081 tgcatcaagg caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg gcgcgaccgt
    25141 attgctcagc acccacgccc ttgccgagtt ggacggacac gccgaccgca ttatcgtgga
    25201 ttaaatttaa tccactatat gcgggtatgg cgggtttgag cggacaaatc agcctgaccg
    25261 tccccgtttt gctgaccgct caggttttat gggttatcat tccgcttgtt ttggcagccg
    25321 gaatttttag aaagcgacaa atatgaaaaa aaccctgttg gcaattgttg ccgtttccgc
    25381 cttaagtgcc tgccggcagg cggaagaggg gccgccgcct ttaccccggc agattagcga
    25441 ccgttcggtc ggacactatt gcagtatgaa cctgaccgaa cacaacggcc ccaaagccca
    25501 gattttcttg aacggcaaac ccgatcagcc cgtttggttc tccaccatca agcagatgtt
    25561 cggctatacc aagctgcccg aagagcctaa aggcatccgc gtgatttacg ttaccgatat
    25621 gggtaatgtt accgattgga caaatcccaa tgccgacacg aagtggatag atgcgaaaaa
    25681 agccttttat gtcattgaca gcggttttat cggcggtatg ggtgcggaag acgcactgcc
    25741 gttcggcaac aaagagcagg ctgagaaatt tgcaaaggat aaagacggta aggttgtcag
    25801 tttcgacgat atgcctgata cctatatttt caaataaatc caaacaaaaa tgccgtctga
    25861 acgttgaagc caaggctcag acggcatttt ttgtcatagg ttcaaacggg gcaggtttga
    25921 acaactgccc cattccttat tcctgaccgg gcgtaacggc ggaataaggt tcacatcccg
    25981 ccgtaattcg gcccgcttgc gccttcggga caaatccaag tgatgttttg cgtcgggtct
    26041 ttgatgtcgc aggttttgca gtgcacgcag tttgccgcgt tgatttgcag gcgcggattg
    26101 ccgttttctt caacaatttc gtacacgccg gccggacaat agcgcgtttc gggcgaggcg
    26161 tattctttgt agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
    26221 tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
    26281 acgccgtcgg gtttcggata atcaatcggc ttacacgcgg cggctttttt aagctgctcg
    26341 ttgtctttgc cgtgatgttt caaggtccac ggggctttgc ctctgaaaat catctgatcg
    26401 atgccggtgt agattgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
    26461 gcggcgtaaa gctcttgata cagccagctt tgttcaaaac gttgctgata atccgccgcc
    26521 tctttgccgc tgtcgaaacc ctccacttct tcaaggtttt ccaacaaggg gaacacggct
    26581 tcggcggcga gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg cggcatattg
    26641 aggaaacccg ccgcatcgcc gaccaaaatg ccgcctttga acgagagctt cggcaaactt
    26701 tgcaaaccgc cttcaatcag cgaacgcgcg ccgtaagcaa tgcggcggcc gccttcaaag
    26761 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
    26821 ggattttgat agtccaaacc gaccacgaag ccgacggcga ctttgttgtc gtcgaaatgg
    26881 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcaccacc
    26941 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
    27001 gtttgcggct ggctgttttg gtcgagttgg aaacgttcga tgatttgttt ggaaagcgaa
    27061 ccgcgacaac cttcggcaaa cagggtttgc tgcgcccaaa gctccatgcc gggttggaat
    27121 gaatcggtcg gctcgccgtc tttgccaatg cccatattgc cggttgcaat gcctttgacc
    27181 gaaccgtctt cgtgatacag cacttcggcg gcggcaaagc ccggatagat ttccacgccc
    27241 atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
    27301 ccgtgattgt cgaaattcgg ggtaatcggc aggttgaacg cttttttctc ggtcaggaac
    27361 aacactttgt cctgcgttac tgtgcgtgtc agcggtgcgc ctttttcttt ccagtcggaa
    27421 atcaactcat tcagcgcaat cggatcgata actgcgccag ccagcgaatg cgcccccacc
    27481 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgttttgttc ggcaagctgc
    27541 ttgagtttga tggcggcaga caaacccgac gggcctgcgc cgacaatcac gacatcgtat
    27601 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
    27661 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
    27721 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
    27781 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
    27841 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
    27901 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
    27961 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
    28021 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
    28081 agggcatagc ctttcgcctc aaaacccaaa tcaggcatga tgcgccgcat atcctcaaac
    28141 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
    28201 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
    28261 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
    28321 agcaacagga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
    28381 ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt tttgttaatc cactataggt
    28441 ttccgtgcgg acgtgttcag attcccgcct tcgctggaat gacggcggag cgatttctac
    28501 ttttccgata aatgaccgta acttaaaatc ccgtcatccc cacgaaagca aaaatcccgc
    28561 ctgtcggatt tcggtttttt tgggcgtttc gggaaactta taaatcgtca ttcccgcgca
    28621 ggcgggaatc cggtttgctc ggtttcggtt tttcgggcgt ttcgggaaac tgatgaatcg
    28681 tcattcccgc gcaggcggga atctagaacg cgggacggcg gcaatattca aaggttgtct
    28741 gaaaattcag aggttctaga ttcccacttt cgtgggaatg acgggatata ggtttcccta
    28801 cggacgtgtt cagattcccg ctttcgcggg aatgacggcg gagcgatttc tgcttttccg
    28861 ataaatgacc gcaacctaaa acccatcctt cccgcaaaaa cagaaaaaca aaaacctaaa
    28921 atcccgtcat ccccacgata acagttgcgt aattgcgtag agtgggcttc agcccaacgt
    28981 tttttctttt tcggtcgttg attggtgggc tgaagcccac ccttgtatat cggaactccc
    29041 gtatcatagc aacaaaccgc ccggccgcca cccgcgccca cccaaggcac acaaccgttg
    29101 cgcagcacag ggagcggcag ggcaacccat cgacacaacc ggacagttgc cggacaacac
    29161 aaccgaatgc aaggcaggtt gatgatgagt acccgatacc attacgcagg tatagtgaat
    29221 taaatctaag gggctgtact agattagccc taaatcccac aggattttaa gctgttgaga
    29281 cggtgtgccg aagttaaatc gaaattcgca ttctttcaag aacagcggga aagatttgcg
    29341 atcgattcca ttgtattttc gcaagacgcg ttttgcctga ttccaaaagt tctcaatgcc
    29401 gttaatgtgg ttctgacggt ctgcacactc cttggaatgg ttgatgcggt aatggataaa
    29461 accgctcacg tccaacttgt cgtagctgct cagactatcg gtataaacaa tactatccgg
    29521 catgattttc tttttgatga cagggagtaa cgtttcagac ttggcattat ccaccacaac
    29581 ggtatagacc cgtccgttgc gtttcagaat gccgaagaca accacttttc ctgccgcacc
    29641 gcgaccacgt ctgcctttac gccgtccgcc gaaatcgctt tcgtcctgct cgacagggcc
    29701 ctcaaaaacc tcatcggcag ccaagaccaa atgatggttg ataaccgtgc ggattttacg
    29761 gtagaacagt gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt
    29821 aacttcgagt acaaaaaaac ggagcagttc tttctgtact tttttcttta atttgcagtg
    29881 cgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc caaaaatata
    29941 ccaaaaacag caaaacaaat tgtaaggata cgtataggct ttgtaaaggt aaattgtgaa
    30001 aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct gatgccctgt
    30061 ccttcccgta tatcttgtgt gttgtcaaag tgcaggctgc tttgaaattg gcattacagt
    30121 cgatgaacca ccattttgct ttatttcagc gggcttgaga tgtatataag aatattgttt
    30181 tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa agcgtagagt
    30241 gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct tgggtataga
    30301 gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt ctcttctatc
    30361 ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc taagtagtaa
    30421 aagttagtaa attattttta a
//
LOCUS       DAWFIT010000016        29438 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFIT010000016 DAWFIT010000000
VERSION     DAWFIT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.016"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..276
                     /locus_tag="WGQ85_000677"
     CDS             <1..276
                     /locus_tag="WGQ85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750154.1"
                     /translation="QKANEQEPFIAAVAETLSRTMWGYVMRMDARFEVTFIVDLGHVA
                     FEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGIAACRVVFGDVL"
     gene            273..554
                     /locus_tag="WGQ85_000678"
     CDS             273..554
                     /locus_tag="WGQ85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750155.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            554..769
                     /locus_tag="WGQ85_000679"
     CDS             554..769
                     /locus_tag="WGQ85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750156.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            772..1278
                     /locus_tag="WGQ85_000680"
     CDS             772..1278
                     /locus_tag="WGQ85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0750157.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1275..1469)
                     /locus_tag="WGQ85_000681"
     CDS             complement(1275..1469)
                     /locus_tag="WGQ85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750158.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            1481..3100
                     /gene="terL"
                     /locus_tag="WGQ85_000682"
     CDS             1481..3100
                     /gene="terL"
                     /locus_tag="WGQ85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0750159.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3179..4738
                     /locus_tag="WGQ85_000683"
     CDS             3179..4738
                     /locus_tag="WGQ85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0750160.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLRAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            4731..6077
                     /locus_tag="WGQ85_000684"
     CDS             4731..6077
                     /locus_tag="WGQ85_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ0750161.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALALPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6181..6678
                     /locus_tag="WGQ85_000685"
     CDS             6181..6678
                     /locus_tag="WGQ85_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0750162.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            6887..7951
                     /locus_tag="WGQ85_000686"
     CDS             6887..7951
                     /locus_tag="WGQ85_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0750163.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            7973..8875
                     /locus_tag="WGQ85_000687"
     CDS             7973..8875
                     /locus_tag="WGQ85_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0750164.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            8913..9386
                     /locus_tag="WGQ85_000688"
     CDS             8913..9386
                     /locus_tag="WGQ85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750165.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9383..9808
                     /locus_tag="WGQ85_000689"
     CDS             9383..9808
                     /locus_tag="WGQ85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0750166.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            9792..10460
                     /locus_tag="WGQ85_000690"
     CDS             9792..10460
                     /locus_tag="WGQ85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0750167.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10457..10645
                     /locus_tag="WGQ85_000691"
     CDS             10457..10645
                     /locus_tag="WGQ85_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0750168.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            10645..12072
                     /locus_tag="WGQ85_000692"
     CDS             10645..12072
                     /locus_tag="WGQ85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0750169.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELAAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12086..12460
                     /locus_tag="WGQ85_000693"
     CDS             12086..12460
                     /locus_tag="WGQ85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0750170.1"
                     /translation="MSTEYVGSVILYLGANEVEVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            12636..13022
                     /locus_tag="WGQ85_000694"
     CDS             12636..13022
                     /locus_tag="WGQ85_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750171.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13009..13473
                     /locus_tag="WGQ85_000695"
     CDS             13009..13473
                     /locus_tag="WGQ85_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ0750172.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            13655..13834
                     /locus_tag="WGQ85_000696"
     CDS             13655..13834
                     /locus_tag="WGQ85_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750173.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            13963..14352
                     /locus_tag="WGQ85_000697"
     CDS             13963..14352
                     /locus_tag="WGQ85_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750174.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPTVTDTPS"
     gene            14310..14558
                     /locus_tag="WGQ85_000698"
     CDS             14310..14558
                     /locus_tag="WGQ85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750175.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            14555..16604
                     /locus_tag="WGQ85_000699"
                     /pseudo
     CDS             14555..16604
                     /locus_tag="WGQ85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            16604..17971
                     /locus_tag="WGQ85_000700"
     CDS             16604..17971
                     /locus_tag="WGQ85_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0750176.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            17955..19094
                     /locus_tag="WGQ85_000701"
     CDS             17955..19094
                     /locus_tag="WGQ85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0750177.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19095..19724
                     /locus_tag="WGQ85_000702"
     CDS             19095..19724
                     /locus_tag="WGQ85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ0750178.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            19785..20138
                     /locus_tag="WGQ85_000703"
     CDS             19785..20138
                     /locus_tag="WGQ85_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ0750179.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20135..20620
                     /locus_tag="WGQ85_000704"
     CDS             20135..20620
                     /locus_tag="WGQ85_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0750180.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            20623..21198
                     /locus_tag="WGQ85_000705"
     CDS             20623..21198
                     /locus_tag="WGQ85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0750181.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21195..21761
                     /locus_tag="WGQ85_000706"
     CDS             21195..21761
                     /locus_tag="WGQ85_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0750182.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            21764..22999
                     /locus_tag="WGQ85_000707"
     CDS             21764..22999
                     /locus_tag="WGQ85_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750183.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVV"
     gene            23162..24046
                     /locus_tag="WGQ85_000708"
     CDS             23162..24046
                     /locus_tag="WGQ85_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ0750184.1"
                     /translation="MNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIGAN
                     AMNKALTAGYSTIIGADAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLASSE
                     KCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAVNI
                     RSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREWWA
                     NPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKAIQ
                     EMAARIETLETEMKESKK"
     gene            24043..24666
                     /locus_tag="WGQ85_000709"
     CDS             24043..24666
                     /locus_tag="WGQ85_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750185.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            24667..25155
                     /locus_tag="WGQ85_000710"
     CDS             24667..25155
                     /locus_tag="WGQ85_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750186.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            25503..26342
                     /locus_tag="WGQ85_000711"
     CDS             25503..26342
                     /locus_tag="WGQ85_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0750187.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            26602..27135
                     /locus_tag="WGQ85_000712"
     CDS             26602..27135
                     /locus_tag="WGQ85_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0750188.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27198..28382)
                     /locus_tag="WGQ85_000713"
     CDS             complement(27198..28382)
                     /locus_tag="WGQ85_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0750189.1"
                     /translation="MTEPTSRRRFLKTCTAAIGAGLLQACGTSATSVPPLPSFHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(28422..28826)
                     /locus_tag="WGQ85_000714"
     CDS             complement(28422..28826)
                     /locus_tag="WGQ85_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0750190.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7504 a         7661 c         8232 g         6041 t
ORIGIN      
        1 caaaaggcca acgagcaaga gccgttcatc gcggcggtgg ccgaaacttt aagccgcacg
       61 atgtggggct atgtgatgcg gatggatgcg cggtttgaag taacgtttat cgtagatttg
      121 ggccatgtgg ccttcgagca tcaattcact gcggtcaaac aggcgctgaa ggaaaaattc
      181 gaggttgaag ccgatgggga tgcgctgact gtggaatccg accgcctgcc ctatggtatt
      241 gcggcctgcc gggtggtgtt cggagatgtg ttatgagcgg agatacaccg attaccgtgg
      301 aatatgtgtt cggcacgttg gtgtcgtttc tgattgcttt attgtggtat tgggtaaaaa
      361 gcatttcaga cggcctgaaa gaagcccgca aagatcgtga tgaactgctc ggcaggttgc
      421 acagcgtaga aacatcttat cagacgaaag cggaagcaaa agacaacagg aacgaaatct
      481 taaacttgtt gcgtgaaatc aaggctgatt tgaaagaagt cggccagaaa atcgaacgga
      541 aggcagacaa ataatgcaaa accaagaccc tattttaaga gcgttggcag aaatcaacgg
      601 caagcaggac aaattgttac aaaatcagga acgcatggat gcggaaatca agaaaatcca
      661 tgccgattgc cgccgcacgt cggcaaccac cggcgcggcc gcgggcgcga tttcgggcgg
      721 tattgtggct acgggcatcg catttgcccg cgccaagttg gggctgtaac gatggcgcat
      781 ccgaaggcga cccgcgacaa gctgcgggca ctctactgca acggcgagca gagccttgag
      841 acggcggcgg ctttatgcgg cgtatcgctc ggcaccgccc gccgctggcg cgatgaggcc
      901 aaggcgcagg gcgacgactg gaacaagctg cgcgcggccc atacgctggc cggcggcagc
      961 atagacgaaa tcgcgcgggc gatgatgacg tcgtttctgg tgcagtatca ggcgacgatg
     1021 acgatgttgc aggatgcgga agtggaagat ttgccgccga gcaaacgggt gcagctgctg
     1081 gcgagtttgg ccgatgcgtt taccaaaacg gtggcggcga acaagcgggt gttgccggaa
     1141 acctcgcaac tggcgacggc tttggagttg ttgcagtttt tgatggtatt cgtgcaagaa
     1201 aaacacccca aacatttggc tgcctttgtg gaagtgctgg agccgttcgg ggcggaggtg
     1261 gagaggaagt ttggttagtt taatcgtgaa gtaaacgtgt catcagaatg tttaccggca
     1321 acaccaattg caaccaatag atctagatga tcggctaatt ttttcagtga aattttaaag
     1381 ccttgctcct ccaataaatc ttgtatttca gacggcgtgt atttgcccgc atctaacagt
     1441 ttggatattt ctaaatcaat cgatttcatt ttttgccctt atgaaaaata aagatttcct
     1501 caaatccctg tccgccctcg ccgccaatct gcgccaagtc atcgaggccg aagtagacgg
     1561 tttcgatgct tcgccgacgg cggtggccga gcggcgggcg aaggtatttg acccggtagg
     1621 cggttacgag tatttcgtca acacctactt cccgcattat atccgttcgc ctgaaaaatc
     1681 cgacctgcac cgcttcttat tcaggcgttt accggaaatc gtcgaatccc cggaaggcga
     1741 gaacgaagcg gttggcgcgc cgcgcggcga gggtaagtcg acgcaggtga cgcagttgtt
     1801 tactttgtgg tgcattgtaa cgggacgcaa gcattactgc gtgattgtga tggacagcat
     1861 agaccaggca tacccgatgc ttgaggccat caaagcggaa ttggagttta atccccgcct
     1921 gaaaaccgat tttgcggatg tctgcggcca aggccgcgtg tggcaggcag gtaccatcgt
     1981 aacggccaac gacatcaagg tgcaggtagc gggcagcggc aaaaagctgc gcggattgcg
     2041 ccacggccct taccgccccg acctgacgat tttggacgat attgaaaacg acgaacaagt
     2101 ccgaaatccc gaacagcgcg acaagctcaa tgcgtggctg acgaaaaccg tgctgccgct
     2161 cggtggtgtg gggcaaaaat acgatgtgat ttatatcggc acgattttgc attacgacag
     2221 tgtgttgaac cgaacgctca ataatccgtt ttggaaaggc atcaagttta aagccatgtt
     2281 gaaatggccc gaccgcatgg atttgtggga cagatgggaa gagttgtacc gcaatgaagg
     2341 tgagttggtg gccgatgcgt tctatcaaac gaacaaagcg gaaatggaac gcggggcgga
     2401 aacttcttgg gcagcgcgcg gcgtgctggc tttgatgaaa atccgcgccc gcgacggaca
     2461 cgcggctttc gattcggaat atcagaatga tccggtgagc ggtgaagatg caccgtttgc
     2521 ccaagccatg cagttttggg cggaactgcc cgccgatttg gtgtatttcg gcgcgctcga
     2581 cccttcgctc ggcaaagcgg gggccagccg cgacccgagt gcgattatcg tgggcgggta
     2641 tcaaagaagc agcggcaagc tgtttatcgt ggaagcgcaa attaagaagc gtttgcccga
     2701 tttgattatt gaagacgtta tccgcttgca tgcgcaatat aagtgcaagc tgtggtttgt
     2761 ggaaacggtg cagtttcagg agttcctgaa agacgagctg gtaaaacgaa gtgcggcaaa
     2821 aggcattccc gtgccggcgc gggcggtcaa accgattgcc gacaagctgc tgcggattga
     2881 gactttgcag ccgcacatgg ccaacgggtt gattttactt catgccaatc agcaaacctt
     2941 aatccaacag tttcgccatt ttcccaaggc agaccatgac gacggccccg atgcggtgca
     3001 tatgttgtgg tcgggggcaa cggccaacag tgcgccggtg gaatatatgg cggtgacgaa
     3061 acatgggacg gacggcggtt tcggcagcgg cgcatggtga aactgacacc gggcagggct
     3121 tacgattaat gcaggcagca gagaattggg gcaagtttaa cttgcccttt ttttatttat
     3181 gaaaaacatt ttcagcagtg cattgagcaa aattctgccg ggccgctata caccgaaatc
     3241 taccccgcaa acggcggaaa ttacccaaaa cacccagact cacgaacacc cgagcaaagg
     3301 tttgacaccg cagaagctgc acggcatttt ggaagctgcc gagcgcggcg atatgaaggc
     3361 gcagtcggaa ctctttgccg acatcgagga gaaagacggc catatctttt ccgagatgag
     3421 caagcgcaag cgggcggtca tcgggctgga ttggcgcgtg atgccgccgc cgaacagcac
     3481 cgacgccgaa cggcggctgg ccgaagaagt caagggatgg attgagcgtc tgcccgattt
     3541 cgaagacatg atgttcgacc ttttagatgc ggtcgggcac ggctttgcct gtgtggaaat
     3601 cgaatggcag caaataggcg gcctgtggct gccgaaaaac tttatccacc gtccgcaagg
     3661 ctggtttaag gtggacggtg ccgataatgt gcggttggcc aaacaggata atccggatgg
     3721 ggaagagctg tgggcgttcg gctggctggt acacaaacac cgcagccgtt cgggtttgct
     3781 ggtacgcggc gggctgatgc gcacgctggt ttggccgtat ctgtttaaga attattcggt
     3841 gcgcgatttg gccgagtttc tggaaatcta cggcctgccg acgcgtttgg gcaaatatgc
     3901 ggtgggggcg gacgaaaccg ataaaaccac gctgctgcgg gcggtaaagg aaatcggaca
     3961 caacgccgcg ggcattatcc ccgaaaccat gaatatcgaa ttgcttaatg ccgccaacgg
     4021 cagcagcgag ccgtttatgg cgatgatcga ctgggcggat aaaacatcgt cgaaagcgat
     4081 tctgggcggt acgcttacca gcatggccga cggtaaaacc agtaccaacg cgctgggtca
     4141 ggtgcataac gaggtgcgcc atgatttgtt ggtgtcggat gccaagcagc ttgccggtac
     4201 gataacgcag caactgattc tgcccctgct gcggctgaat aaaggcaacg tggatgaaac
     4261 ctgcctgccg cgtttccagt ttgatacgca gctacccgaa gatatggcgg tttacgccga
     4321 atctttgcct aggctggtag agatgggcat gaagattccg ctggcgtggg cgcaggaaaa
     4381 actggcgatt ccgctggctt cggacgacga gccggtattg gctttgcaga cggctgaaag
     4441 cgaaggtgtc aaagttgcgc cgttaagtta ccgccgcgtg gccttgagca ggcagggcga
     4501 aatcttggat atggggcagg cggccatcga taacgcaggt ttgggcaaaa tcgccctgcc
     4561 cgagcatatt gagccgtttt tgcgcgggtt gggtcaggct ttggccgagg gcgacagcta
     4621 tgaagaggtg caggagcggc tgttgcgtgc ttatccgcat ttagacagcg ccgaatttca
     4681 gacggccttg gcgcgggtga ttttcatctc cgacctgtgg gggcggctca atggctgatt
     4741 tgggttttgc cttcggtttg gagccggaag cggcggtcaa atattttgaa ggtttgggct
     4801 accatatccc gcccgactgg gatgtgaaat ggaatgaggc gcagaccaag gcgcggacga
     4861 ttgcaggcat acacaggcag gatattgtcg gcgagtttca cgcggcaatg tatgaggcgg
     4921 cgaaatcggg caaatcgttt gaggcttggc gcgatgaagt gcaaggccgt ctgaaagcgc
     4981 atgactggca tctgctcaaa gacggcgata ttgtggacgg agacaccggc gaagtcctcg
     5041 gacgcggtat taccaaacac cgcatggaaa cgattttccg cactcagatg caatcggctt
     5101 acatggccgg tcattggcag gcgtttgaag aaggtcggga tgatgcgccg tggctgcaat
     5161 attcggccat tttggacagc cgcacccgcc aaagccatgc ggcggcgcat ggtgcggtgt
     5221 atcacatcga cgacccgttt tgggattact tctacccgcc caacggcttc aattgccgct
     5281 gtaccgtgcg ggcgctttca gacagtgatt tgaaacggcg caatctgctg ccgcaaaaag
     5341 cgcagcttga agatacggaa gtggtggtca accgcaaggg cgatacccgc cccgccaaag
     5401 cggtaaagct gccggacggc cgccgctttt ataccgatgc gggctttcag cacaatgtcg
     5461 gtaaaagcca tttggccaac ttggggcagt tgcagatgca gcgtgcagtg gaactgccgc
     5521 cgaaactggc gagcgtatcg attcagacgg ccttaaaaca gcctgatttg atgcgggcgg
     5581 tatcgcaaca ggcggcgcaa atggtacggc gggtggatgc ggaaaaagta gcgcgcggga
     5641 aaacgctgta tgtgggcgca ttggccctgc ccgtgttgga cgcattggcg gcaaggaaaa
     5701 tttacccgca atccgccgtg attgccataa gcgacgagcg ggtattgcac gctttgcgcg
     5761 acaagaaggt caagccgctg ccggtatcgt tttgggagaa gatacccgaa ctactgcaag
     5821 agccggagca gattttattg ggcaaggcgg gacgaaatga cgatgccaaa cagtttttgg
     5881 catttgtgta tccgctgtcg gcaggcaagg ggaagctggt ggtaacgctg gactatgatg
     5941 tgaaaacccg acatccgttg acaggtaaga aagaacatct gaccttgaat atggtcaaca
     6001 ccggcatgat tgcagaaacg gataaacagg tggatagctt actgtacggg tatgaaacaa
     6061 tatggaaaaa gccataaaac tctttgcctg attcgaacag gataatacgc gacacggtaa
     6121 cgtggccgta acctttccag taggaaaccc gagttttatg gctgttggaa ccaatatacc
     6181 atgattgaga taaaaatcaa cacagacacc ctgcaaaaca gcttaaacgc tgcggcgcgg
     6241 catacctccc acaccaagcc tttgatgacg cggcttgccc gcatcatgcg caacgccgta
     6301 ctggagaatt tcgccgccgg aggccgtccc gcttgggcgc cgcgcaaata tccgactgcg
     6361 cgtgaaggtt cggggctgtt gcaagccagc ggccgcctgc gcaattcgat tacgccgagc
     6421 agtaccaacg atacggcggt ggtcggcacc aatgtggaat atgcggctat ccataacttc
     6481 ggtgggaaaa cctcgccaca cttgattaga ccgaaaaaag gcaaagcgtt gaaattcggc
     6541 ggccgctttg ccaagcaggt taaccacccc ggcagcaata ttcccgcacg cccgtttatg
     6601 accctgcaac cggaggacga aaaggcccta tccgatgcgg tggcggaata tctggcacag
     6661 gccattcagg ggcggtaatc atgcctgcct gaaaaatgcc gaaaaacagc ccgaaaacgc
     6721 cctaacccat acttacccct acccatcgcc tgaaaatcaa tcctgcgcgc gtttgaacac
     6781 cttttgaaca ctatcccacg cgtatctctg caagtacatt tcccttccct agaaattcat
     6841 ctgtctgaca taggccaagc tttgacgagg gcgcggctgc cgcacaatgc gggctatgga
     6901 caccaaaacc cttcttgccg ccttatccgc cgcccatgtc ggcggttcgg acggcttaat
     6961 caaaatcgta cccaaaggcc aattcgcacc ggttgacggc cgcaccgata cgggtgtgcc
     7021 gcactggacg atgtctgccg atttggcaca gcaaatcatt gccgcctttg acgccgcgca
     7081 aaccgacctt gtggtcgact acgaacacgc cacgctgaaa gccgccgaaa cggggcagca
     7141 aaaccctgcc gccggctgga tcagcaaata tgtatgggat gacgagcgcg gcctgatggg
     7201 cgaagtgaaa tggacgcaac gcgccaaaga catgatagac agcggcgaat accgctatct
     7261 gtcgccggta ctcgaatacg acacgctggg caatgtacgc gggctgcaca gtgtggcgtt
     7321 gaccaattcg cccgcactgg acggcatggc tctggctgca ttgagccgcc aaaactctat
     7381 caaccccaaa caggaaacaa gtatgaacaa ggaagcttta atcaagctct tgggcttggc
     7441 ggcggatgcc gacgacaaag ccatcgaagc ggctttggcc gaagcacagg aaaagctggg
     7501 cggtaaaacg cttgccgaag cactggccgc acccaaagaa gaaccgcaag gtagcgaagg
     7561 cgataaaggc gatgccggca aacccgaaga caagccgcaa ggcggcaatg ccgacgacgg
     7621 cgaggtagcc gaactcaaag cgcaagtggc cgcgttgagc aaaaaagtga ttgcaatgga
     7681 agtgggcggt acttcagacg gcctgatccg tgccgcgctt tcagacggcc gcctgttgcc
     7741 gcatcaagaa gcatcggcgc gccaactggc cgccaaagac cctgaagcgt ttaaagccct
     7801 gattgacggc agcttggcac tggccgcttt gagcaaaacg cagaccggcg gcaaaggcgg
     7861 cgcagacggt acgtccgcgc tgaccgccga agaagcagcc gtcgccgcgc aattgggcat
     7921 ctctgccgaa gattatgcga aggccaagta atccgataaa ggaaaagaca acatgattat
     7981 cacaccagac accttaaaag ccctgttcac cggctttaaa aagaatttcc aagacggcct
     8041 gaaaatggcg gaaagccaat acaaggaaat cgccaccgtc attccgtctt ccactgcgtc
     8101 caatacttac ggctggctcg gccaatggcc cgctttccgc gaatgggtgg gcgaccgcgt
     8161 attccaagat atgaaggcgc acggctatgc catcaccaac aagcattttg aaagctcggt
     8221 caaggtcaac cgcaacgaca tcgaagacga caacgtcggc atttacgcgc cgatgatgac
     8281 cgagatgggc cgtgcttcgg cggtgcatcc cgacgaattg gtgtttgcct tgctgaaaaa
     8341 cgcacacgcc acgttgtgtt acgacggtca gaacttcttt gataccgacc atcccgtgta
     8401 tgaaaaagtg gacggcaccg gtcaagccag taccgtatcc aatattttcg ccggcagcga
     8461 agcggcctgg tatctgctgg acactacccg cgccctgaaa ccgttgattt accaagagcg
     8521 taaagcgaag cagtttaccg ccatgaccgc cgataccgac gaaggcgtat tcatgcgcaa
     8581 cgaataccgc tacggcgtgg acggccgttg caacgtgggc ttgggcttct ggcagatggc
     8641 ggcgaaatcg caagagaaac tggacgctgc cggtttcgag aaagcctaca acgcgatggt
     8701 gagcctgaaa ggcgacggcg gcagaccgct ggccatccgc ccgaatgtgc tgctggtacc
     8761 gcctgctttg gaaaatgcgg ccaaagagct ggtggaaggc gaccgcctgg ccaacggcgc
     8821 gtacaacccg aacaaaggca aggcgaaagt catcgtatcg ccttggttgc tgtaacgaac
     8881 aggcgggcac cgcccgccgg aagggataga aaatggcaaa agaaaaaaac ggacaggaag
     8941 ttggcgcgac cgtcgatgtc caaccggaag aggtaaccgt aaccgctgcc ttacaggccg
     9001 agattgaagc cttgagagcc gaattagaca aagccaatgc cgaaattcag gtagcgcagg
     9061 cagagttggc cgccgcgcaa gagcgcaatg ccaaattgga aagactgctt gaagccggcc
     9121 ttgcacccgg cggcatttca gacggcgaaa ctttgacacc tgaagcggaa gccgcctttt
     9181 acaccggcag cggagcaccc gccgcagatg ccgaagtggt ggcgattaag agcaaacacg
     9241 gccatgcgtt tttccgcgcc ggctaccatg tacagccgaa ctggacgttt gtgcgccgtg
     9301 ccgacttcga acccgccgac tttgagcgcc tgattggcga ccgtatggtt gaagcgcgcg
     9361 aagccctgcc gacggacgca tcatgagcta cgccgccgtt gccgatttgg tggcgcgttt
     9421 cggcgaagcc accatcaccg gcctgaccga cttgtcccgc aagggcgcgg tggacgaaac
     9481 cgtcgcacag caggctttgg atgatgccgc cgccgaaatc gacggctatc tgatgaaccg
     9541 ctataccctg cctctgccca agccgctgcg catcttaacc gtgtattgct gcgatattgc
     9601 ggtgtaccgc ctgtgtaccg gcaaacggca gcttaccgaa gacattgtgc accgctatga
     9661 agcggcagtg aaattcctgc aactggtggc ggcggggaaa gtcggcttgg gcgttaccga
     9721 acccgcaggc gaaaagcccg ccgtacaagg caacggcgtg atgtttacca cacaggaaaa
     9781 ggtgttcggt cgtgatagcg tctattgaac aagccatcag gcagcggctt tcagacggcc
     9841 tcggccaaat ggtaacgggt gtctttactt acggcggcga gttcgacggc gaaggtttgg
     9901 cgcaggtggt caaccagttc cccgccgtgt gggtcatgtt tgccggcatc aaagacacca
     9961 tccgccacga tacgcgcggc agccgtttta aagcgattgg ccaattcacg gtattggtgg
    10021 gagaccgtgc cagcggcagc gaagcagaca gccgtttcgg cggcctgcac cgccatgatg
    10081 tcggcaccta ccgcctgatg cagacctgcc gccacctgct gaccaatcaa tcgctgggct
    10141 tacagataga ccgtctgcaa ccgggggcgg ccaaaagcct gttcagccgc caaatggagc
    10201 aagacgccgt tagtgtgttt gcgctggaat ttgaaacgta ttggtttgaa gacgcgctgc
    10261 aagacggcga ttggccgcgg cctgcggttt cagacggcca acaggcgaaa gtatatgccg
    10321 acgtatccga ataccaaggc cgcaccgaac ccgaacatcc cgactttaaa ggcgtgaacc
    10381 ttgaattgcg tatcccgccg aaaacccccg accaacccgc cgatatggcg gccaccgttg
    10441 aaaccaaggt gaaaccatga acgaaaccat taaagtacgc gccgccgacg gcctgcaagt
    10501 gccgctggcc ggtaaaccac atgaatacat taccgaccaa gaaaccgtta ccgtacccga
    10561 tgccgcctat taccgccgct gtatcgaata cggcgacctt gtggtcgtaa ccgaagacac
    10621 ccaacccgcg aaaggcagca aataatggca tccgcaaaca tcaatttcga aaaaatcccc
    10681 gccagcaccc gcaagccggg cgtgtacgcc gagtggaaca ccaagctggc cgtgcgcaac
    10741 ctgcccacca acaaacagcg cgtgttgatt gtggcgcagc acaacaatcc cgctttgggt
    10801 gagcttaccg agctggaaaa cgtgttttcc gccgccgatg cggccgccaa atacggcgca
    10861 ggcagcatgg cgcacctaat ggttaccgcc gcgattaaag cctatgccta tgccgattta
    10921 agcctgatta ccgtagccga caacaaggcc ggagtggccg ccggcggtaa aatcacctta
    10981 agcggtactg ccaataccca aggcgtgttg cgtgtgagca ttgccaatgc tgacacttta
    11041 accatcggca tcggcgccga agacaccgca gccaccgttg ccgccgccgt caaagccgcc
    11101 atcgatgccg tgcccgattt gccggttacc gccaccgttg ccgaagcagt ggtgacgctt
    11161 accgccaaaa acaaaggtac ggccggcaac gccatccgca tcaaaaccag caataccgcc
    11221 gaaggcatta ccgccgccgt taccgccatg acgggcggcg atgccaaccc cgatattgcc
    11281 gccgcgctca atgccgtagt cgccgaaggc caccacatca tcgcctgcgg tatcaacgac
    11341 gaaaccaacc tgctgaaact gcgtgcccat ttggataccg tagccagccc gatggaaaaa
    11401 cgctgggcaa tatgcgtata cggccaaacc ggtacgctgg cgcaagccac caccttggca
    11461 ggccgtctga accacggcca tatcgtcagc gcgtggtatc gcggcacccc cagcctgcct
    11521 tgcgagctgg ccgccgcctt tgccgctgtg atggcgagcg aagaagaccc cgcccgcccg
    11581 ttgaacacgc tggcattaaa cagcatcggc gtgtgcgaaa gcaaagacaa aaccatgcgc
    11641 accgagcagg aaaacgccct ttataacggt gttaccccga ttgaaaccag tccggccggt
    11701 acgcaggcgc aaatcgtgcg cgccatcacc acctacacca aaaccgccaa cggcacggca
    11761 gacgaaagcc tgctggacgt aaccaccgtg cgcaccctga tttatgtaag ccgcgcctgc
    11821 gtcgaccgca tcgccctaag attcccgcgc gacaaattga gcgaccgcac cccgccgcgc
    11881 gtgcgcagcg aactgattga cgtattgatg tgctgcgaag agctggaaat tttggagcgc
    11941 gtggaagaaa acctgccgaa actgattgtg gaacgcgacc tgcaaaacac cggcatgttg
    12001 aactgccgca ttcccagcga tgtcgtcaac ggcctgcatg tagtaggtat ggttgtagat
    12061 ttatatttgt aagaaaggct gaaacatgag taccgaatac gttggcagcg tcatcctcta
    12121 tctgggcgcg aacgaagtcg aagtaaccaa aatcgacgtg aaagacaaca ccggcaaaaa
    12181 gcccgtaaaa accatgaacc gcacccgccg cgtcaaaggc tttacccgcg gcgtcggcca
    12241 atacgacatt acctttaccg ccgtggtgcc gaccgacggc acggcggtgg actgggcaaa
    12301 gatcgacgac gccaaaatca gcctagttcc cgacgtggaa ggcgcgcgcc cgacatcgta
    12361 cctcggcttc tgcgccaccg aagcgggtga cagctatacc gtagataatg agctggtggt
    12421 ggatgtgacc ggttttgcga ttcggaaggt gttggagtaa agattttctt gttaacagaa
    12481 gccgctgaag cggcttctgt tgtttttatt acactgtttt taataaaatt caatttttca
    12541 agtaaaatac ttgaatttta aaaaagcctt tgatattatt tagccacgtt gaatgtggca
    12601 gtaatgctgc aaacacatga aaaggagctt gaacaatggg actgactaaa ttcggcgcag
    12661 ccgttcgtga ggctcgtcgt caaactaaac aaactctgca aacgatggct gctacattag
    12721 gtacttctcc tgcttttttg agtgctattg aaactggtcg tagtaaagtg ccaatggatt
    12781 ttgttaaaaa ggttgaagat ttttttgaaa acttaggcca accaatagac ggtttaaagc
    12841 aaaaagcgat ggtttctaat gagaatgtat cgttaagcgg cttaagcctg caacaacaaa
    12901 tgctggtggc tggttttgcc agttcagact tcagcaaaga gcaattggac aaatttgctg
    12961 aattattaag aaccattcat cataaagatt cacagaaaga aggggatgat gcagcaaact
    13021 aattattcaa tgcgtggtgt gcgtgtggga atgttaagcg aagaaacgat tcagtttttt
    13081 gcaaacgaag ctgccaaatt tttggtagta gataagcgta ctcgtaaaaa aatggatgca
    13141 tttatggaaa tgctggaaga gtatggcatt gtaattgatg ttgttgctga ttctgaatgg
    13201 ctagtcatta ccaacgcaat gtgccataac ggaacgattc tgatgcctaa tagcctctat
    13261 actcgcattt gcaatggcga ggacgaggct atctttattt tctttcatga attggggcat
    13321 ttgcttttag ggcataaagc catgttgcat tatagtgaag ttcttcccac gaaacaggaa
    13381 gattcagaat ggcaagctga tgagtttgca aaatgtattt taagaaacat gggaattaaa
    13441 tatgtaccag aacagttaag cttacgattt taaaaagaga aatgacttgg aatgttggcg
    13501 catcccaagc cattattgtg gtgtactagc atacttccaa tatgctgtac aagtgtagtt
    13561 ctccatccgc taactgcaaa cagatttcaa agagaaatac ttgaaaggga cttgagtata
    13621 tatttttcag atttgtttgt ccacgaaaag cgaagtgatg gccaccattt ttacaattgg
    13681 aggtgccatt atggctatga ctaaggctcc cgttaccccc gacttggtgg acgatacagg
    13741 tgcatacatt tttagaaagt cttttaccac caaaagtggt aagaaaattg tttctaaaaa
    13801 tgggaagcca tttaaaattc ctgtgaaagt ttaggaatat ataagccccg ctaagcgggg
    13861 ttttttcatt actgacgcgt gtttagtcgc ccatctcatc tccttaagtg acaattaagc
    13921 cttgatttat acaaggttta accaacgttt aagggctttt ttatgtctga attttccccc
    13981 gaactcaccc gcgctatcga agattacgaa ttacgcgttt ctcccgacct gaaaaccgtt
    14041 gccggccgtc tgaaatacgg catcggtgtg attgatggcg aaatgcacca tgatttttcc
    14101 atgcaccttt taaccgtgcg cgaggacatg gcgattgatc cgcaacttga aggccagccg
    14161 cggctggttg ccgcctatgc cgcgtcactg gataaattgg gtggtttaac agccgaatct
    14221 ttgacacctg atttgctgct tgatgagatg gctgctgctg actttgacgc gctgtactgg
    14281 gcgcaagagc tgctgcaaaa aaagcggctt tgcccgcacc ccgcgccgac cgttaccgat
    14341 acgccgtctt aaaactcggc cgatacggca tcacgcccgc ccaaatcgac ggcatgaccc
    14401 aacccgaact ggaaggctgg ttaaaacagg ccgacttaat cgagcgcggc cgtgccgcgc
    14461 cggtagtgat gccgtggttt attcctgcga acgtcaaacc atctgccgcc acgggcggtc
    14521 agacccaaac ctttatcagc aagcgtaaga aaaaatgagc cagcatagtg tagaactggt
    14581 tgctaagttt aaggacaacg ccacgcccgg cctccgccgt ttggcggcgg aagcggaaaa
    14641 aacaggcagc cggcagttgc agaaagccgc agcagtcaag ctgaaacatc aagagatgta
    14701 cagcgctacc gcccgtttgg gcatccgtac tgaacaccaa atccgccgtg aaatccaaca
    14761 aacgcaggcg gcttacaacc gtttggccaa aagcggcatg gcttcgcaac gcgaactggc
    14821 gagggctgca cagataacgc gcagccgtgt acgcgagctg actgccgaaa tcaacggcgg
    14881 tgcaagccgt ttgcaacgta tcggttcggg agtccggaca atcgggcgcg gtgtggccgg
    14941 cgtggcggcg ggtgcggcgg caggagctta tgtattggcg cagccggtca accggacgat
    15001 ggactttgac acatcattgc gtcatgccgc caataccatg tatgcaggca agagcatggc
    15061 cgagaagcgc gcaggtatgg aggaaatcaa gaaatcggtc atggatgccg cctatgtcgg
    15121 cggaacgacg cgtgatgccg ctttggaagc catgaataca atggtggcca gcggagcgat
    15181 gagcgacgaa gccgtgaaaa aactgctgcc gaccgttatg aaaaccgcca ccgccgccaa
    15241 tacggaagga aacgatattg ccaacatcgt aaccaaggcg ttgcaggcgg ggtttaaaga
    15301 ggcggatatt ccgtcgctgc ttgaccgtgc gttgcaatcg ggcgcggacg gcggttttga
    15361 attgaaagac atgtcgcgct ggctgccgca gcagttggct gcaatgaaaa acgccggtat
    15421 gggcgcgacg ctggataatt tttccagcct gctcaatgcc aaccaattgt cgtttatgac
    15481 ggcgggcagt accgacgaag ccggcaacaa cttagtcaac ctgctggcaa aaatcaacag
    15541 tcaggatatt gtgaccaaag ccaaaaaaat taccatcaac ggcaaagaag gtttcgattt
    15601 caccgctagc atgaacaaac ggcaggcagc aggcatgaac tcgcttgatg cgctggtgga
    15661 cattgtcgga gaaatcacgg ccaaggataa gaagtccgcc gctctgatga aacagattaa
    15721 tgccgcgcaa ggcgatgaag ccaagctggc tttgctggaa aaccaaaagg ccttggtaga
    15781 cggcactgcc atcgggaagc tggtttccga ccgtcaggca ttgatggcat tgctggcatt
    15841 ggtcaacaac aaacaggaac tcacccgcct gcaagcaggc cagacaaatg cggcgggtgc
    15901 ggtggacggt gcatacggat tcgtcgccga aggttcgggg tttaaaaaat cccaattctc
    15961 cctcgccaaa tcagaagccg aatacggcgc atttgaaaaa ttcagcgacc gcatcggcgg
    16021 cggccggtac aagctctatg tttgggggcg gcggcctgct ttccaaactg ttcggcggaa
    16081 gcagcagaac cttacaggcg ggccgtctga tgccctcggc ctcctcgcta ggccccgtcg
    16141 cgctcggtgc gacaccgttg gccgtaatgg gcggggcaac ccatttggcg gggcagcgcg
    16201 acaaatatgc ggaatggtca aaaccgtttg ctaaattatc gtcttggctg gaaagtatcc
    16261 tgcctgattt ttcagcgggg gcgaaagccg aatatctgcg caaacgtgaa gaactgggcg
    16321 gcaataatgc cccgctcgat agtcctgttt taaaagagag tatggcgcag cttaaccaat
    16381 cggcacaaac caaccagcag gcaagccagc agtatgtgca ggcggcggcg gagaatcagg
    16441 cggcaacggc gcagattacc ggcgcggcgg cacagatgac ggctgctgcc gcacaaatgc
    16501 aggcggcggc aggcaagccg attccgatta ccgttactgt gcaaaacggc aacattatgg
    16561 cctatgtgaa tcaggcagta gaacgcaaca gtaggaaaaa ttaatggctt ggaaagacac
    16621 tttactggat gcgggcttca agggcgttac ctttgacgtc atcgacgaca cgctgcgcgg
    16681 cacccacgcg ctggcggaac acgaataccc gttcgtacag ggcgcggaca tcgaagatac
    16741 gggtgtatct gcgatggata tggccttgac cgccgtattg tggggtgaag attacgaagg
    16801 ccgcctgcaa aacctgctga acgtattgtg cgaaacgggc gcgggcgaat tgatccaccc
    16861 gatttacggc agcgtgccag attgcgtagt ggcggatttc gaggtcgcgc acaacgaaga
    16921 aaaccccgac tactgcaccg tgcggatgac ctttaagcaa agtgtcaaag ccgcgccgtt
    16981 tttcgaccgc gacttgccga tagccttggc cgacgaagtg gatttccttg ccgacttggc
    17041 cgcatggcag ggcttcgagg tgtttcagac ggccttaaac aaaattcaga aagcgcaaag
    17101 ccgctggaat gcttttcatg ccacggtatt aatggtggtg ggcgtattgt atgggcaggt
    17161 aaacggcatc ttcaccggcg gcctgaacct gctgaacagc ccgcgcgtat tggtggcgga
    17221 gctgaaatct gtgtttggtg cgctggccaa tatgcacaat gtcggtaaaa gcgggctgga
    17281 cggctggcgc gacatggtgg gcggcgtatc taaagcggcc gctacgccgt ggcaggtcag
    17341 ccgcggaacc gaaggcagtg tggcggcggt agatttaatc caacgcgcca agcccgaaga
    17401 tgtggctgcc tttaccgcgc ttaccgccac cgttggcgcg tgtgcgctgg ccgaacaagc
    17461 cgccgatatt ttggccgtac aactgaacga gccgactttg acgcctgtgg aaatctcgcg
    17521 tctgctggcc gatacccgtg ccgccttgca gcgtgctttg gccgcccagc gcatcttggc
    17581 catgatgctg gcggatgaaa ccaaagccga caagctggcc tattgcctgc tgcggctgta
    17641 tcaaacaccg gtagacagtg ccgatgatgt gtaccaacgc atcgaagtgg ccgggctgct
    17701 gccgcaagcc ccttatctgg aaactgccgc cgcgcttacc gaaagtctgc gcaatacggc
    17761 gcataaactg caaaaacagg cgtttacagt catcaatatg aagccgccgc tggtacagaa
    17821 aaccgtcacc tgcgatatcg gcctgcacct gctggccttt gagtggtacg gcgactacag
    17881 ccgctttggc gaactcttgc gcctgaatcc gcaaatccgc cacccgaatt ttattgagaa
    17941 aggcacggta ctgaatgcct acgccagata acaccgttac gctgctgatt aacggtaaaa
    18001 cacacggcca atggacgaat tacgacattg tttccgacct gctcaccccc gccgatgact
    18061 tttcagtcat gctcggccgc cccgtcgatg ccaaacccga tgcggtgcgg gcgggcgaca
    18121 aggtggaagt gcgtgtgggc ggcgatacgg tattgagcgg ccgcatagac cgcgtgcaga
    18181 ccgttaccga aaaaggcggc aaaaccttaa ccatacaagg ccgcgacgat gcgggcgtgc
    18241 tattggactg ctccgccccg ctgtttaacg cgcaagatat ggatttaaac caaatcatcg
    18301 aaaaaatcgt caagcctttg ggtttggcca aaatccgcat tgatgccgcc aaaaccgaca
    18361 aaacccacaa agtgcagatc gagccgggca gccgcgcgtg ggacgccctg ctggaatatg
    18421 ccgaggcaaa cggtttgtgg ccgtggctgg agccggacgg cacgctggta gtcggcggtc
    18481 ccgattacac cgccgcaccg gtggccgaac tggttttacg gaccaacggc caaaacaaca
    18541 acatcaagcg tctggaagta aaccgcgata tggcggcacg gtacagcgaa gttaccgtat
    18601 tggcgcaaag ccacagcggc aaaaacaaca tcaaagccac cgccaaagac gaatccgtca
    18661 aactgcaccg ccccctaatc gtcaccgagc cggacatcga cagtcaggct caagcgcagc
    18721 gcaaggcgaa aaagcggctg gccgacagca ggctggaagg cttaacaatt accgccaccg
    18781 tacaaggcca ccgcactgac gacggtacct tgtggcagcc cggtcagcgc atcaacgtat
    18841 tgagcgaacc ggacggcatc gacgcggtgt atttcctgat ggcgagaacc ttcatcggcg
    18901 gtcgcggcca acccaccgaa accgtgctga cactgaaaga ggacggcgca tgggtattgg
    18961 atgccgaccc gccgaagaaa tcgggcaaaa ccaaaaggcc gtctgaaagc cgcaaagcca
    19021 acggccacgc agccgccaaa ccgaaaaaac gccgccaagc caagcaggcc ggacaggaat
    19081 tgcaggtgat ttaaatggat gcaaaaacca tagacaaccg tatccgacgg gcatttaacg
    19141 gcatccgcca agccttccgc ggcaaaatcg cccgcgtcaa agcagccggc ggcgtgcagc
    19201 aaatccaagt ggaaggtttg gaaggcgaaa ccgtgcagga cttggaacac gccgaaaact
    19261 tcggttttac gtcgaacccg cccgcaggca gcgattgcgt cgtcgtaccg ttgggcggca
    19321 aaaccagcca cggcatcatc gtcaccacca ccaacggcgc ataccgcatt accggccttg
    19381 ccgaaggcga aacggcggtt tacaacgccg acggtgccaa gatggtgtta aagaaaggcc
    19441 ggattatcga aattgactgc gaaacgctga atatcaaagc accgggcggc gtgaatattg
    19501 acgcgcccaa tgtaggctgc accgcgcaga ttaccgccga gggtcaaatc aacggcaacg
    19561 gcggcatggc agtgaaaggc ggcagcggca catcatttac cggcaatgtg cagatggtcg
    19621 gcgatttgga cactaccggc aaactgacca acaacggcaa aaatgtcggc tccgaccaca
    19681 aacacaacgg cgacagcggc ggcacgacat ccgacccgct gtaattcaga cggcctgaca
    19741 tccatcaaac caaactccaa gcgtccttac ctgaaaataa aggtatggac gctttactta
    19801 atccccaaac gggcggctat gtggtcaacc aatccgccca atccatcgaa aacgagttgt
    19861 atatccgctt ggtcacgccc ttgggcagtt actgggcaga ccgcacgctc ggcagccgcc
    19921 tgcacgaatt gcgccgccaa aagcatttaa agcgcatcga agtactggcc aaacagtacg
    19981 ccgagcaagc cttgcagccc gtgattcagt ccaaacgcgc ccaatccatc caagtaaccg
    20041 catccgcccc gcagcacggc tggctgaagc tgcatattga agccgtcgat gccgcgggcg
    20101 atacagtaac cctgaaccac aaggtagccg tgatatgacg caggcactga atttagaaca
    20161 aatccgcgcc aactatctgc gcgacctgca aaaccaaaac cctgccgccc atgtacacgc
    20221 gggcagcgac aaccatgtac gcgccaccgc cattgccgca gtgggcgaag gccaatacca
    20281 gcatcaggag tggattttgc gccaagcctt tgccgatacc gcagacagcg cctacctaga
    20341 aaaacatgcc gctaaatacg gcatctaccg aaaaaccgcc accttcgcgg gcggcaaggt
    20401 gcgtgttcgc ggcgcggtcg gcgcaacggt gccggttggc caacagatca atgtgggcga
    20461 taaggtgtat ttaaccgccg aatccgccgt tatcagtgcg cttggaagtg ccgaaatagc
    20521 cgttatcgcc actgttgcgg gtagtgctca aaatcaaacc gccgaaaccg cggctacgct
    20581 gcaaagcgta cctgcgggga ttgacagttc cgccgtctaa cgatggtcgg cggtacagat
    20641 gccgaaagcg atgagagcct gttggcacga tatgaagaac gtctgcgccg acccgctgcg
    20701 ggcggtaatc aatacgactt ccgcaattgg tgcttggaag taccgggtgt ggttgatgcc
    20761 tttatctacc ctttgcgtcg cggcaacggc tttgtcgatg ccgtcatttt gggtgaaaac
    20821 ggtatcccca gcgcagaaac actggccgcc gtacaagctc atgtcgatgc ggtgcgacct
    20881 gttacccgta aaaacggctt tctagccctt gcgcccagta tccagaccgt gaatgtggcc
    20941 gtcaccatta ccttaagcag cggcacggat accgatacgg ccaccgctgc catcaaatca
    21001 gctgtaaacg cctattttga tgccttaaag cccggagacc ccctaattaa aagccagttg
    21061 gaaaccttaa tcagcgaagt gtacggcgtg cgtgaccgtg tcttaaccac ccctgtgggc
    21121 aatatcaagc ctcaggagag tgccgaagac atttactggc tgcgcccggg cagtattaac
    21181 gtggagtaca ccacgtgatc catcaaacct tactcgccgc catgcgtccg cccgtcagct
    21241 acgacaccgt aggagaaacg gcagaaatca aagccgaagc gggtgtgttt gatattgtgg
    21301 ccgaccatgc ggaaggagtg aaaaatgcgc cgttccctga tgcggaaaac gattacctgt
    21361 accgctggga agagctgctc gccatcaccc cgcccgcagg agccaatacc caacaacgta
    21421 ctgatgccgt gctggccaaa ctcaatgcct tgggcggttt gagtattgcc tactttaccg
    21481 ccatcgccga atcggcaggc tacaccgtca ccatttacga agaagaccaa ttccgcgcag
    21541 gtgaaagctg tgcgggtgat tgtttgaata ccgaagacgc catctggcgt tggtgcgtcg
    21601 acatcgccga cggcaaagcc accgcctata ttttccgagc cggtcaaagc cgtgcgggcg
    21661 accgcatcag tgtgtacacc gacccgatta tcgaaacgat gtttgaagaa ttaaaaccgg
    21721 catggacgta ttgccgcttt gaatatgaag aagaggtata aaaatggact taatccaaac
    21781 cccgaataag caatttgtcg acggcgaccg ccgcacgccc ggtactcccg tacccgcatg
    21841 gtggctgaac cagttacaag gcgagttgta cagcatttta aacgcggttg gcattgagcc
    21901 taacaaagcc gaccatgccc aagtcttatc ggccattaaa acgttggccg ccgatgcttc
    21961 gcaggttgcc agtatcgatg ctctgcgtaa atacagcggc acaggctatg tgaacgtcaa
    22021 cgcctatcac gccaatacaa cagtgggcgg cggcgtgttt gtggcggata aagccgataa
    22081 atctaccgct gataacggct gtaccgttat tgtttctacc gacggcacgc gctggaagcg
    22141 tgtgttttca gggatgctta acctgcatga ttttggatat gtggccagca aaaacaatgc
    22201 actatccact ttgaatgccg ctgaatctgc cgcgcttgac gtagttgttg attgcttggg
    22261 tttgtcaatt gatacgggta atacctaccc gcaaaaaaac aaatacacaa acggcaagtt
    22321 tgtgattaac ggcaaaactg tcgatgttca ataccagcct atcagaagcg gtatcggtcg
    22381 attcatctcc ggaactggtg cagcagccaa cctcaaatcg aatgaatgga ctggcgcggg
    22441 tttaatcgtt attggcgaag gcgcaatgga gcagatggag aaatgtgttt cctcaatcgc
    22501 tattggcgac cgtgcgcagg gcttttctaa agtaagcagg gacaacatcg ccattggggc
    22561 cgacagcctg attaatgtgc aggccgctac tgaatggtac gaccagtcac gcatggaagg
    22621 cacgcgcaac atcggtattg gtggtaatgc aggacgcggc atcaccagcg gttactctaa
    22681 tgtgtcaatc gggcgcaatg ccggacaggg attgggtgaa ggctcgtcaa atattgcact
    22741 tggcgcaggc gcgatggctg gtactgctcc agtcggtttt agtggcgaca ttgaagtttt
    22801 ttggccgtct tcgacctcaa gaacaatcgc aatcggcgag gctgtcttgc aaacatatca
    22861 gggccgcgcc gctcaaaccg caattggtgc caatgcggcg cgaaatacaa aaaaggccga
    22921 aaaagttacc gcaatcggtt ctgccgcgat ggagaatctt gagcgaaacc gcgccccaaa
    22981 tggcggagat gttgtctaga cgggaacgga agcaggtacc tacgcccaat ctggaaaaaa
    23041 catcacgctt acatttccca acattcgcgg tgcgcaagcg acttattggg tgggcatccg
    23101 ccttacatca ggcacggcgc aaaccttaca aaacgacgtc gtaccggctc aggtcgtatc
    23161 agtgaatggc aatacattaa tcatccaaag ctcaaaagag ctgaccgcca ccggcgcggc
    23221 cgaactgaaa tacgtttatt ctgtaaattc aaccgctact aaaaacgaag agttgaccat
    23281 catcggcgcg aacgccatga ataaggcatt gaccgcagga tactcaacta tcatcggcgc
    23341 agatgccgcg ttgttgggag acaattatca aaaaacaacc gcaatcggcg catcatcttt
    23401 acgaacaggt agtcatattt ccacaactgc tattgggtat tgggtaatcc ctttggcaag
    23461 tagtgagaaa tgtgttgcca ttggagatag tgcgggctat cggaacgttc aaggcgactt
    23521 tttgactggg aaaataacaa actccatcgc catcggatat ggcgcaagaa taaacggcga
    23581 taacgaaatc caaatcggta cgacagggca aactttatat gctccaaccg cggtgaacat
    23641 ccgttctgac ggccgcgaca aagcagatgt taagccgttg acgaacggtt tagattttgt
    23701 aatgaagctc aagccgatga ctggctacta cgaccgccgg gattcctacg ttgacgaatt
    23761 attcaaagac ttgccggcag atgaacgagc ggacaaagtc cgcgaatggt gggcgaatcc
    23821 aatcaaggac ggcagtcata aagaagatcg gttgcggcat tggtttattg cccaggacat
    23881 tgctgcgctg gaagatgaat atggtcgatt gccgatggta aataaaacaa acgataccta
    23941 caccgtcgaa tacgaaacgt tcatccccgt tttgactaaa gccattcagg aaatggccgc
    24001 aagaattgaa acattagaaa ccgaaatgaa ggaatcgaaa aaatgacaag atgtgtgatt
    24061 gatcaagacg gcttgttcgt agaggaacag tattttgatg acggccgcca aagcatcgaa
    24121 gctgaaatgc ctgatctcgc acaatatcag gccgcccagt gggatggaca aggttggaag
    24181 ataattcccg actatcgcgg atgcgtagtt tttgccggtg ggcaagagca ggtgtgggat
    24241 aagttaggtg atttgcccga tggcgtcagc ctgaccccgc ctgaatcggt aaatattgac
    24301 ggcttaaaat ccgtaaaact cgtcgcatta aatgctgccg ctcaggcttt tattaacaag
    24361 cacgccggta tcgacagcgt acctgaattt gagtttgcaa gctgggcaat tcaagccgct
    24421 gaagcgaagg cttggcagga agataaagcc gcgccaacgc cagtgcttga cggcattgcc
    24481 accgcccgcg gtatgtcagc agacacgctt aaagcagcgg ctttgcgtaa aacgctggct
    24541 tacgaacaac tcgccgcaca tgtggcaggt caacgacaag cgctgcaaag caaaatcgaa
    24601 gcagcgaaaa cgcaggccgc gcttgataag attgcagtcg tattcacact gccggaggcc
    24661 gtctgaatgg ttcaagtcta tttggcactc tataaaggca aagccgcaat caacaccccg
    24721 cgcgatgtgg ttaaacgcat tgccgacagc gttgtacgat tggcaacatg cagcccgtac
    24781 agccattgtg aaatcgctgt taagcaccca cgcgacggcc tgtttgattg ttattcgtct
    24841 agcgcgagag acggcggggt gcgcattaaa accatgccgc tgcctgctga taaatgggac
    24901 ttaatcccgc tgccgcaatc tgttgccata tcggccagcc gcttgttcca ccgtacacac
    24961 ggtgcaggtt acgactggct aggtgcgatt ggcgtggtac tcaaatcacc acacagcaaa
    25021 agccgctggt tttgcagcga atggtgcgca tatgtaatag gctacactaa cccgtgccga
    25081 tacagcccgc aaaccctgta tgccgcggta tcaactaaag ataggccgtc tgagaaaatg
    25141 gaggaaacaa agtaatttag aaagttttaa ataaagagga gcggcgacgt gtctgtgttg
    25201 cgagcaccgg cacgccagcc aagcagatgt accctgcatt gacttcaagg ccgctttgcc
    25261 tagctaggcg gcggtaattc taacctaaac ggagttaatg cgacatggtt tattatcgtg
    25321 aattacgttg tgtctactgc aaaaaaacta ttggccaaag gcagcggtaa cgtacaaatc
    25381 aagtgtaacc gttgtaaaac ggttaatact ttcagctaga caaccattac taagaatgcc
    25441 gtcgagcatc attttaaaac tgatttcaga acaccagcga gagtgtcgga gagtaagtaa
    25501 aaatgatgca aaaataccac tcaacggccc cgctgccatt cgtcggacag aagcgatatt
    25561 tcattaaaca cttcactaaa gtattgtcgc aaattcccgc tgacggcaaa cattggacaa
    25621 ttgtagacgt attcggcggc agcggcctgt tggcgcacgt tgcaaaacgt atcaaaccgc
    25681 aggcgcgggt aatttacaac gactatgaca actactcaga ccgcctgcgg cacatcccag
    25741 attacaaccg tttgcgtgaa caaatcgcgc agatagttgg cggcatcccc aaaggctcaa
    25801 ggctagaccc tgaacgtacc cgatcagtgc aacaaacaat cactaatttc caaggccaca
    25861 ttgatgtgcg cgtactttca tcatggcttt tatttagtgc caaacaggca aattcgcttg
    25921 aacaattgct ggggtttgag ttctacaata aggtacgcca atccccatac tccatcgctg
    25981 ccgactattt agacggcctc gaaatcaccc agcaagacta taatcttttg atggctgagc
    26041 atcagcataa ccccaatact ctgctggtat tagacccgcc ttatgtgtcc actgctcagg
    26101 gtgcgtatgc cgctgataaa tactttaata tggttagctt cctgcgtatg attcagtata
    26161 tgcgcccacc gtttatccta tttagctcca cccgtagcga ggcgctggac tacttccaat
    26221 ttttgcaaga gtgcgaaccg gacaaatacc ggcgcttcag cggctacaat atcgtttcac
    26281 tagatgccaa gatgggcaaa ggaatcgagt atcaggacaa tatgatttat aaaatagatt
    26341 aacccgccta aacggcgggc ggtatcaaag ttccacctta tagcgcggcc taactaggtg
    26401 tcaaagattt tccgaaaagg tgctaaagtt cgcgccgcct tataaactgc acccgttttc
    26461 gggtgcagtt ttttattgcg gattgggacg ggaatggaaa aggatgtgga ttttttattt
    26521 taatcggcta tacaattgct gctttcatgc cgtctgaagc acgtttcaga tggcatcggt
    26581 ttacatcaga taaggataaa aatgggtatt tacgattttc aaatgaaaga tgcagaaggc
    26641 aatgcggttg atttgtcagg ctatcgcggc aaggttctgc tgattgtcaa cacggcaacg
    26701 cgttgcggtc tgaccccgca atacgaggct ttgcagaagc tgtatgcaca atataccgca
    26761 gaaggcttgg agattttgga ttttccgtgc aaccagttcc gcgaacaggc tcccgaaagc
    26821 agcggagaaa ttgcccaagt gtgtatgatg aagtttggca cgaagtttaa gattttcgac
    26881 aaaatcgaag tcaatggagc aaataccgcg cccttgtatg cctacctgaa atccgtcaaa
    26941 ccgcaagata aaggcaatca tctgtttaaa gatttcgtgt tgaagctcgc agctttggga
    27001 gagaagcgcg acgaaggcga catcaaatgg aactttacca aattcctcgt caaccgcgac
    27061 ggcgaagtgg tcgagcgttt tgcgcctagt gttacgcccg aagaaatcga agccgatatt
    27121 cgggcattgc tgtaatctgc cgccgtcttg ggaaatgccg tctgaaggct tcagacggca
    27181 tttgtccgtt tgcgggttta ttccgaaata tcggcaacac cgctttcggg gaagatgggc
    27241 aaatccggcg gtgggagcag ggtatccaaa gtcagggcgg acgcatcgag tgtggggtag
    27301 ccttcaaacg cgaccgaata accgctgttt ccgttcatac ggattcgggc gtgcgcgccc
    27361 agagggaaag tgattttgtc ggcaatgtgc ccgaagggaa agcccgtcag cacggggatt
    27421 ttcgccgtgc gcgaaacatg gttggcaacg gcggaaaaat cataagacgg atcatagaca
    27481 tctcgaattt tttccatacg gaaattgccg aacacgatgg cgcgctgttt cttcaaaata
    27541 cccgaaagat acagcgtatt gagcatacgt tcgatgcggt agggctgttc gccgacatct
    27601 tcgaggaaca aaatgccgcc gtcgatgtcg ggcatataag gcgtgccggc gagcgaggcg
    27661 aggacgctta agttgccgcc ccacaatatg ccttcggttt cgacatcggc gcgttggata
    27721 taaggaacat caacggtcag gcggttttgg gttgcaccct tgataaaggc atccatcgta
    27781 aacgcaccgg gggcgggttt gccaaaatcg ctataagcca tcgggccggc aaaactcatc
    27841 atattgcctt ttgccaacaa tgccagctgg acggcgcata cgtcgctgaa tccgaaaaac
    27901 agcgtgccgt gttcgcgcat ccttgcgccg agcgaagcaa aatcgatatg cggcagaatg
    27961 cgtgccgcac cataaccgcc gcgcaagccc atcagcactt taggtgtggc aacgcgaccg
    28021 gaagcgacat cttggaaatc ggcggcacgt tgtgagtccg tgccggcaaa ccgttggaaa
    28081 cggcggctgc ccgcctgttg gttggttacg gtaaaaccgg cattgtaaag gcgggttaag
    28141 gctgtgttga cgcggttggt gtcttcggca aagcctgacg aagcgacaac gcgcagaagg
    28201 ttgccgtccg aactttgacg gcgtggcgtt tggagaggca cggttcgggc tttcacaacg
    28261 gaatggaaag agggaagggg cggaacggat gtggcggatg tgccgcaagc ctgaagcagt
    28321 cccgcgccga tggcggcggt gcaggttttc aggaaacggc ggcgggaggt cggttcggtc
    28381 ataatttccc tttcatacgg tttcagacgg catcggcggg cttaaagcag ctttttaagc
    28441 tgttccgtcc aatcttgcgg cagggtcata ccgtgtttcc ggacggggtt tgcccaaatc
    28501 ggtgcaggga aagacgcatc gttttcaaag cgggcgataa tatgccaatg caggtgcggc
    28561 acgacattgc ccaagctggc gaggttaatc ttggcaggtt gaaacacttg gcgcatagcg
    28621 gcttcgactt tgtacaccat ttccatcaat tcgccgcgtt ccgctgccga aaggtcggtc
    28681 atttcggcaa tatgcttacg ccaaatgacg cggcagaatg caggcgaacc gctatcatta
    28741 tggacggcga tgacgcgcag cttgggggtt tgcagcaaaa cgtcttcatt ttgcgccgtg
    28801 cagatggggc agggttgggc ggtcattttt ccttattcct gaaagtgttt aaaaaacggt
    28861 agggtaattt ctgatatagc ggattaacaa aaatcaggac aaggcaacga agccgcagac
    28921 agtacaaata gtacagaacc gattcacttg gtgcttcagc accttagaga atcgttctct
    28981 ttgagctaag gcgaggcaac gccgtactgg tttaaattta atccactata gtttaatttg
    29041 tctgtccgca ttgccgctaa ttgtaccact tcatcgtatt tccttttcgg ttgaaacccc
    29101 gccctttagg gcggcaggat cagactttat ttgggagggg gtgtaacccc ttccaaatca
    29161 ggacgacaca tagggaggtg ctttatgtgt cgtcctgtgt gttgaaacat atcggacgcg
    29221 tttgaacagt tgtgcggcaa actgttttag cggcaggcaa gagatgccgt ctgaagccca
    29281 ttgtccgtca ttcccacaca ggcagttgct acggttgctg aagcccctta gccgtcgcgc
    29341 aggcggttgc tacggttact aaaatcccgt cattcccgcg caggcgggaa tccagtccgt
    29401 tcggtttcag ttgtttctga taaatcttgc agcgttgg
//
LOCUS       DAWFIT010000017        26619 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFIT010000017 DAWFIT010000000
VERSION     DAWFIT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.017"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(717..1523)
                     /locus_tag="WGQ85_000715"
     CDS             complement(717..1523)
                     /locus_tag="WGQ85_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0750191.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
     gene            complement(1565..1660)
                     /locus_tag="WGQ85_000716"
     CDS             complement(1565..1660)
                     /locus_tag="WGQ85_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0750192.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            complement(1672..2511)
                     /locus_tag="WGQ85_000717"
     CDS             complement(1672..2511)
                     /locus_tag="WGQ85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0750193.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDWMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            complement(2511..3600)
                     /locus_tag="WGQ85_000718"
                     /pseudo
     CDS             complement(2511..3600)
                     /locus_tag="WGQ85_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3617..5680)
                     /locus_tag="WGQ85_000719"
     CDS             complement(3617..5680)
                     /locus_tag="WGQ85_000719"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0750194.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGVVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(5701..6111)
                     /locus_tag="WGQ85_000720"
     CDS             complement(5701..6111)
                     /locus_tag="WGQ85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0750195.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(6188..7093)
                     /gene="glyQ"
                     /locus_tag="WGQ85_000721"
     CDS             complement(6188..7093)
                     /gene="glyQ"
                     /locus_tag="WGQ85_000721"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0750196.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEGASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            complement(7416..7838)
                     /locus_tag="WGQ85_000722"
     CDS             complement(7416..7838)
                     /locus_tag="WGQ85_000722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0750197.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(7849..9246)
                     /gene="atpD"
                     /locus_tag="WGQ85_000723"
     CDS             complement(7849..9246)
                     /gene="atpD"
                     /locus_tag="WGQ85_000723"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0750198.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(9284..10159)
                     /gene="atpG"
                     /locus_tag="WGQ85_000724"
     CDS             complement(9284..10159)
                     /gene="atpG"
                     /locus_tag="WGQ85_000724"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0750199.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(10184..11731)
                     /locus_tag="WGQ85_000725"
     CDS             complement(10184..11731)
                     /locus_tag="WGQ85_000725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0750200.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(11742..12275)
                     /locus_tag="WGQ85_000726"
     CDS             complement(11742..12275)
                     /locus_tag="WGQ85_000726"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0750201.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(12280..12750)
                     /locus_tag="WGQ85_000727"
     CDS             complement(12280..12750)
                     /locus_tag="WGQ85_000727"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0750202.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(12821..13057)
                     /gene="atpE"
                     /locus_tag="WGQ85_000728"
     CDS             complement(12821..13057)
                     /gene="atpE"
                     /locus_tag="WGQ85_000728"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0750203.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(13114..13980)
                     /gene="atpB"
                     /locus_tag="WGQ85_000729"
     CDS             complement(13114..13980)
                     /gene="atpB"
                     /locus_tag="WGQ85_000729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0750204.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(13970..14323)
                     /locus_tag="WGQ85_000730"
     CDS             complement(13970..14323)
                     /locus_tag="WGQ85_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0750205.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(14691..15029)
                     /locus_tag="WGQ85_000731"
     CDS             complement(14691..15029)
                     /locus_tag="WGQ85_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750206.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(15165..16025)
                     /locus_tag="WGQ85_000732"
     CDS             complement(15165..16025)
                     /locus_tag="WGQ85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0750207.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            16184..16753
                     /locus_tag="WGQ85_000733"
     CDS             16184..16753
                     /locus_tag="WGQ85_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0750208.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(16815..17678)
                     /locus_tag="WGQ85_000734"
     CDS             complement(16815..17678)
                     /locus_tag="WGQ85_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750209.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(17838..18524)
                     /locus_tag="WGQ85_000735"
     CDS             complement(17838..18524)
                     /locus_tag="WGQ85_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0750210.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRTIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(18526..19263)
                     /locus_tag="WGQ85_000736"
     CDS             complement(18526..19263)
                     /locus_tag="WGQ85_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750211.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            19793..21643
                     /locus_tag="WGQ85_000737"
     CDS             19793..21643
                     /locus_tag="WGQ85_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0750212.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIEAADLSA
                     SVPTRPVEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVVCYADLKHSDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDSNNAWRRVRGLLANSQTTDARNLAAALGSPFDGSTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVSDRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSHAALGNLQQAAKL
                     YEQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVRRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            21932..22144
                     /locus_tag="WGQ85_000738"
     CDS             21932..22144
                     /locus_tag="WGQ85_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0750213.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            22276..22722
                     /locus_tag="WGQ85_000739"
     CDS             22276..22722
                     /locus_tag="WGQ85_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0750214.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            22743..22892
                     /locus_tag="WGQ85_000740"
     CDS             22743..22892
                     /locus_tag="WGQ85_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750215.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(22921..23313)
                     /locus_tag="WGQ85_000741"
     CDS             complement(22921..23313)
                     /locus_tag="WGQ85_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0750216.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(23384..23989)
                     /locus_tag="WGQ85_000742"
     CDS             complement(23384..23989)
                     /locus_tag="WGQ85_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0750217.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAQLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(24091..25767)
                     /locus_tag="WGQ85_000743"
     CDS             complement(24091..25767)
                     /locus_tag="WGQ85_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0750218.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(25928..26542)
                     /locus_tag="WGQ85_000744"
     CDS             complement(25928..26542)
                     /locus_tag="WGQ85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0750219.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
BASE COUNT         6387 a         6970 c         6971 g         6291 t
ORIGIN      
        1 aataacggaa ttttaagttt taggaatttg tcggaaaaac agaaatcccc cccgccgtca
       61 ttcccgcaaa agcgggaatc tagaaattta acgttgcggt gatttatcgg aaatgactga
      121 aactcaacgg actggattcc cgcctgcgcg ggaatgacga gattttaggt ttctgttttt
      181 ggttttctgt tctcgtggga ataacggaat tttaagtttt aggaatttgt cggaaaaaca
      241 gaaatccccc cgccgttatt ctcgcaaaag cgggaatctg gaaatttaat gttgcggtga
      301 tttatcggaa atgactgaac ctcaacggac tggattcccg cctgcgcggg aatgacgaat
      361 tttaggtttc tgttttcagg tttctgtttt tggttttctg ttctcgcggg aataacggaa
      421 ttttaagttt taggaatttg tcggaaaaac agaaatcccc cccgccgtca ttcccgcgca
      481 ggcaggaatc tagaatttta atgcggcaag aatttatcgg aaaaaaccga agtttaaaga
      541 cctagattcc cgcctgcgcg agaatgacga gattttaggt ttctgttttt ggctttctgt
      601 tttctcggga atcacgaatt agggcttacc cgaaaacaag tgaaaccgaa caaaccggat
      661 tccccgacat aaatttaggg acagcgcaca cccgccaaat aaggaaaggc caaaacctat
      721 tcaaacggga caacgtgccg cccgaggtgt ttttccaatt ttttctgacg tttccgttcc
      781 ctcgcccttt cgatttccct gccgattttt cccaaagcgc gtttcaacaa aaccttcggg
      841 gagcggcggt tgcggtacga ttcctgcctg ccgtttttca gttggctggg cagggtttcg
      901 ctttggacgc acaaggcggg ggtcagttgg caaaccgtca tccccccttt gtcgagaaaa
      961 ctgctgaaga gcatccaatc caccgccttc aggcgttcag acggcaaaac ggcaaacctg
     1021 tccaaaaaga aacgcatcgc ctttcgggaa ataatatagc ccgccatacc aaaatgttcg
     1081 ctttccaaca gcggaaaggc gcgcccgccg tagtccgcca cgccggaggg cgaggtcagg
     1141 acgtgcataa acatcgtttc caagcggacg ataaaggcgg aatccttgtc aaaacgctct
     1201 tgcagccaag cgtcttcggc aaggaatttc tccgcacctt cgccgagtaa aacgtcgtcc
     1261 tcaaatacgg cgatatacgg cagaccttca tccaatgcct gcttccacaa tacggcgtgg
     1321 ctcataaagc aggctttttc cactccgctc aaataggggt gcgccgacaa accgggtacg
     1381 agttccgcca ttgcctgctc cagtgcttca gacggcatca gtgcgtcgaa aaactgaaac
     1441 ggaatgtcgt gcctgccgaa ggtatcggca atgtgcgccc tgcgttctgc agcggaagcc
     1501 aagctgataa cgtggttttg cataatttat cctgtttttt gtctgttgga taaagcggcg
     1561 tttttcaacg gtttttcagc aatcggtgca aaatgccgaa gtattgcctc aaggtaaaca
     1621 gccgccgcat cctgccgtct gccgcaaata cgatgtccat cttttctcct tttattggaa
     1681 aggcacaatg atcttgccga ttaactgttc gcgcctttgc cgacgttttt ccctttccct
     1741 gctgattttg gtcaaggcgc ggatcaggcg gtgtttgaat gtgttggcgg gggaatcgcg
     1801 cctttgctgt ttgcggttca ggaggcggtc gtgttcgatc aggctgccca atgcgctgtt
     1861 ttggtcgtga aacttggcat aatgcagctc ttgggcgcac aaggcgggat cgagctggca
     1921 aaccggcatt ccttccctgt cgaaaaaatc gctgaacatc atccaatcga cggggtgcag
     1981 cccttcgggc ggcagggcgg caaacctgtc caggaaaaac cgcatcgctt ttcgggaaat
     2041 gatatagccc gccgtccccc agtgttcgct ttccaacagc gggaaggcgc gcccgcagta
     2101 gtccgccacg ccggagggcg aggtcaggac gtgcataaac atcgtttcca agcggacgat
     2161 aaaggcggaa tcggggtcaa agcgttcttg cagccaagcg tcttcggcaa ggaatttctc
     2221 cgcgccttcg ccgagcaaga catcatcttc aaatacggcg atatacggca gaccttcgtc
     2281 caatgcctgt ttccacaata cggcgtggct cataaagcag gctttttcca ctccgctcaa
     2341 ataggggtgc gccgacaaac cgggtacgag ttccgccatt gcctgttcca gcctttcaga
     2401 cggcatcagt gcgtcgaaaa actgaaacgg gatgccgcgc acgccgaagg tatcggtaat
     2461 gtgcgccctg cgttctgcgg cggaagctaa gctgataacg tggttttgca ttaaatctcc
     2521 tgttctttcc ctgccgaatc cgaccgcgcc tgccggcggt ttttaatcag ccggtgcaaa
     2581 atgccgaagt attgcctcat ggtaaacagc cgcctcatct tgccgtctgc cgcgaaatcc
     2641 agccacgcgc cggcgggcgg cgtgtccgtc cgtttgaagc attggtacaa aaaccggcgg
     2701 gcgcgttcaa aatcttcttc cggcaaatgt ttctccagca attcatacgc tactgctttt
     2761 atttggcggt attcaaggct gtcgaaccgg gttttaaaac ccatagactg caaaaaatcg
     2821 tttctggcgg ttttttggat gccttgcgcg atttcgtgtt ggcggatgct gtatttggat
     2881 gaaacctgat tggcgtgaag gcggtatttg accaaggctt cgggataata agccagcctg
     2941 cccaatttgc tgacatcgta ccaaaattgg taatcttccg cccaatcccg ctcggtgttg
     3001 taacgcaatc cgccgtcaat gacgctgcgc cgcataatca tcgtgttgtt gtgtatgggg
     3061 ttgccgaaag ggaaaaagtc ggcaatatct tcgtgccggg tcggcttttt ccaaattttg
     3121 ccgtgcctgt gatgccgcgc cagccggttg ccgtcctttt cttccgacaa aacttccagc
     3181 cacgcgccca tcgcgatgat gctgcggtct ttttccatct cgcccacgat tttctcaatc
     3241 cagtcggggg gcggcaatat cgtcggcatc ggtgcgtgca atatattccc ccattcctga
     3301 ctttgccaat tcgtccagcc cgatgtttaa agagggaatc aggccggaat tttgagcttg
     3361 tgcaaggatt ttgatacggc tgtcccgctt ttgaaaatcc ttggcaatgg caagcgtacc
     3421 gtctgtcgag ccgtcatcga caatcaaaat ctccaagttg cgccaagtct gattcacgac
     3481 ggtggctaat gattgggcga aatatttttc tacgttgtag gcgcaaatca atacgctgac
     3541 taaaggctgc aatttattct cccgataggc acgatgccgt ctgaaggctt cagacggcat
     3601 ttggactgta caacggttac tcgcccaaaa gcgcgatgtc ggctaccgcg ttcatttgct
     3661 ctgccaagcg gttcagcagg ttcaggcggt tttgttttac ggcggcatct tccgccatta
     3721 ccatcacgcc gtcaaagaat gcatcgactt gcggtttgac ggaagccagt tcggacaagg
     3781 cggtttggaa attgccttcg gcaacagcgg cggcgatttt cggctgcaag ccttgcgcag
     3841 cggcaaagag ggctttttct tcgtcctgtt gcaacaggct ttcgttaacc acgcccaact
     3901 cggcatcggc ttttttcagc aggttttgca cgcgtttgtt ggcggcggcg agcgcggcgg
     3961 cttcgggcag ctgtttgaac gcggcaacgg cttgcagttt ggcggtcaaa tcgtccaaac
     4021 ggcgcggctg tttcgccaat acggcggcaa cgatgtcttg cggataatcg ttttgcagca
     4081 acacggccaa acgcgcctgc ataaagtcgg cggtttcaga cggcgttttt tcgttgagca
     4141 aacctttggg gaagctgtcg aatgccgtct gaatcagttc gttcacgtcc aaaccatact
     4201 gcatcagcat acgcaaaata cccaaggcag cgcggcgcag ggcgtagggg tctttgtcgc
     4261 cggtcggaat cagaccgatg ccccaaatgc cgaccagggt ttccagcttg tcggcaagtg
     4321 caacggcagt ggcaattttg ctttcgggca gcttgtcgcc ggcaaaacgc ggttggtaat
     4381 gttgctcgac ggcttcggcg atttcttcgg tttcgccgtc caagcgggca tagtatttgc
     4441 ccatcgtgcc ttgcagttcg ggaaactcgc cgaccatttc ggttaccaag tcggctttgg
     4501 caagacgcgc ggcgcgttcg gcagcggcgg catccgcgcc caatgctttg gcgatgtggg
     4561 cggcaatgct ttgcaggcgt tcgatgcgtt cggcttgcga gccgattttg ttgtggtaca
     4621 ccacgctgga caatttcggc aggcggcttt ccaaagtcgc tttttggtct tgtttgtaga
     4681 agaactcggc atcagacagg cgcgcgcgca aaacgcgttc gttgcctcgg atgatgtgcg
     4741 acgggtcttc ggtttgcagg ttggaaacca gcaggaagcg gttcatcagc ttgccgtttt
     4801 ggtcgagcag cggaaagtat ttttggtttt gctgcatcgt taaaatcaag cattcctgcg
     4861 gcacggcgag gaaatgttcc tcaaaaccgg cttccaaaac aacaggccat tcgaccagcg
     4921 cggttacttc gtccaacaat gcttcatcgg cggcgacggc ggcgttcaga ctgcgcgcct
     4981 gcccttccaa tgccgtctga atcgcggctt tgcgctcggc aaacgaagcg acgactttgc
     5041 cttgcccgcg catttgttcg gcgtagctgt cggcgttttc aatggtgatt tcgccgttgg
     5101 acaagaagcg gtgtcccaag gtttgattgc tgctttgcag acccaaaacg ctgacgttta
     5161 cgatgtcgcc gccgtgcagc acaatcagcc cgtgaacagg gcgcacgaag gtaaatgtgc
     5221 tgctgcccca acgcatcact ttgggaatcg gcagtttctt aaccacttga ttgataatgt
     5281 cttccaaaag tccgcccagc ggcttgccgg tttggacgta ttcgtaggcg tacacatcct
     5341 gcttgccgtc gtggacgatg gtcaagtctt cgattttcgc gcctgcaccg cgtgcgaaac
     5401 cttccaaagc cttggtcggc gcaccgtctt ttacggcatt cgccacggca gggccttttt
     5461 tcacgatttt ttggtcggcc tgaacggctt tgacgttttt gacttgaacc gccaaacggc
     5521 gcggcgaggc ataagccgta aattcggctg caccgtcaac cagttgcgct ttttccaagc
     5581 cttcggcaac ggcggcggca aaatggttgc ccagattatt cagggctttg ggcgggagtt
     5641 cttcggtaag gagttcgatt aaaagggttt gggtcatcat tcggctttct ttgaatttgg
     5701 ttaatctgcc tgtttatagg tttcgctgta attttcccag ccgtcatccc cgtaaaaacc
     5761 atcaaccagc ggggtggcgt acaaagtggc gacatcttcg cggcctgcca gccaagagat
     5821 aatggctttt ttctcggttt cgcccaagct tcgggcaccg gatttttgaa acaggcacga
     5881 aaaatcgccg caatcgcccc cgcccgccat ttccaagcca tttgcctcaa gatacgcaat
     5941 cagctcgtcc ataaagcggc caaacgcttc ggcatcgtcc gcagcttggt gcaaactgcc
     6001 ttgaacgccg aaaatcaatg tttgaaactc gcccaaatgc agctttttat gctggcggcg
     6061 gttcattttg tgcaggcgtt tcctgcttgg ggtgcggaaa tagacaggca tgattttcct
     6121 aaaaaatata atggcttccg gacggctgcc ttatcgtgcc gcccgaacgt aaaaaatcgt
     6181 cgccccctta ggcagcattt gccttcatta aagggaagcc cagtttttcg cggctttcaa
     6241 catatttttg cgccacggcg cggctcaatg cacgaatacg tccaatataa gttgcccgct
     6301 cagttacgga aatcgcgccg cgcgcgtcca agaggttgaa cgtatgcccc gctttgagaa
     6361 ccagctcgta ggcaggcagc gcaaggcttg ctccttcaac ggcaagcagg cgtttggctt
     6421 gcgcttcgta gtcgttgaac tggcgcagca gccaatcggc atcgctgtat tcgaagttgt
     6481 aggtggattg ttcgacttcg ttttggtggt acacgtcgcc gtaggttacg gtattgccgt
     6541 ccagcgtttt tgcccaaacg aggtcataga cgttttccac gccttgcagg tacatcgcca
     6601 agcgttcgat gccgtaggta atttcgccga gtacgggggt gcagtcaatg ccgccgactt
     6661 gttggaagta ggtaaactgg gttacttcca tgccgttgag ccagacttcc cagcccaaac
     6721 cccacgcgcc caaagtcgga ttttcccagt cgtcttcgac aaaacggatg tcgtggactt
     6781 tggggtcgat gcccaattcg cgcagagaat caagatagag gtcttggata ttggcggggg
     6841 cgggtttgag ggcgacttgg aattgatagt agtgttgcag gcggttggga ttgtcgccgt
     6901 agcggccgtc tttagggcgg cggctgggtt ggacgtaggc ggcaaaccaa ggctcggggc
     6961 cgagcgcgcg cagacaggtg gcggggtggg aagtacctgc gccgacttcc atgtcgaagg
     7021 gttggatgac ggtgcagcct ttgtctgccc agaatgtttg cagtttgaag atgatttgtt
     7081 ggaaggtaag catggcttat gattcgataa aataaagggt ttattttact gtttccattg
     7141 ctgtttggat aggtttatct caaagacaga ctgatttgaa aacacggcat acatgatata
     7201 gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct
     7261 aaggtgctga agcaccaagt gaatcggttc cgtactattt gtattgtctg cggctttgtc
     7321 gccttgtcct gatttttgtt aatccactat attttcaaaa cttggaaaaa taaaaagcac
     7381 gaccaaacgg tcgtgctttt ccaaaccaaa caagtttatt tcttgtgcga acggatatag
     7441 tccaaagttt tgagctgtgc aatcgcagca gccaatgctt tatgtgcttc cgccaaagcc
     7501 ttatcgtctt tagcttggga aatgcccgct tctgcggctt ttttcgcctc ttccgcacgt
     7561 gcctgatcca tctccgcact gcggacggca acatccgcca agacagttac tttatcaggc
     7621 tgtacttcca aaacaccgcc ggaaacagca accaaaacct ctttatcctc gcccggaacg
     7681 gtcaaacgca aagcccccgg ccgcaccaaa ctcataatcg gctcgtgtcg cgggtaaata
     7741 ccaagctcac cttgaacggt cggaaccact acaaagctgg cttcgcccga atagatgttt
     7801 tgctcgctac tgaccacctc aacttgcatg atgctcatgc cgacctcctt agtttaaggt
     7861 tttcgctttc tctactgctt cttcaatgct gccgaccata tagaatgcct gctcgggcag
     7921 atgatcgtat tcgccgttca agatggcttt gaagccggca atggtatcgc gcagggcgac
     7981 atatttgccc ggagaacctg taaacacttc ggcaacgtgg aacggttggg acaggaagcg
     8041 ttggatttta cgcgcacgca ttacggtcag tttgtcttcg tcagacaatt cgtccatacc
     8101 caagatggcg atgatgtcgc gcaattcttt gtatttttgc agggtggact gcacaccgcg
     8161 cgctacgtcg tagtgctctt gacccaatac catcggatcc agttggcgcg aagtagaatc
     8221 aagcggatcg actgccgggt aaatacccaa agaggcaata tcgcggctca atacgacggt
     8281 tgcgtccaag tgggcgaacg ttgttgccgg agacgggtcg gtcaagtcgt ccgcaggtac
     8341 atatacggct tggatggaag taatggaacc ggtttgggta gaggtaatac gctcttgcaa
     8401 acgacccatt tcttctgcca atgtcggttg gtagcccact gcagacggca tacggcccaa
     8461 cagtgcagat acttcggtac cggccagggt gtaacggtag atattgtcca cgaagaacaa
     8521 tacgtcgcgg cctttgccgt tttcgtcttt ttcatcacgg aagtattccg ccatggtcag
     8581 accggtcaat gcgacacgca aacggttgcc cggaggttcg ttcatctgac cgtaaaccat
     8641 tgccacttta tccaatacgt tggaatcttt catctcgtgg tagaagtcgt taccttcgcg
     8701 ggtacgctca cccacgcctg cgaacacgga caagccgctg tgcgctttgg cgatgttgtt
     8761 gatcaattcc atcatgttca cggttttacc cacaccggca ccgccgaaca gacctacttt
     8821 accgcctttg gcaaacggac acagcaagtc aatcacttta atgcccgttt cgagcaattc
     8881 ggttgtggaa gacagttcgt caaacttagg ggcggcttgg tggatggcac ggctcttgtc
     8941 ggtatcaatc ggacctgctt cgtcaacagg cgttcccaat acatcgacaa tgcgtcccaa
     9001 cgtaccttta cctaccggca cagtaatggg cgcaccggta ttgctcacag tcatgccgcg
     9061 tttcaaaccg tccgagctgc ccatcgcaat ggtacggact acgccgtcgc ccaaaagctg
     9121 ttggacttcc aaagtcagac cgttttcgtc taatttcaaa gcgtcgtaaa cgcgcggaat
     9181 catgtcgcgt ggaaattcca cgtcaacaac cgcaccgata atttgtacga ttttgccttg
     9241 gctcattatc gtatcctaat ttctgtacag gattcagacg gcatcagaca gccgccgcac
     9301 ctgctacaat ttctgacaat tccgtggtaa tcgcagcttg acgcgatttg ttatatacca
     9361 gacgcaactc tttgatggca ttgcctgcat tgtctgttgc agctttcatg gcaaccatgc
     9421 gggctgcctg ttcggatgcc atattgtcgc tcaacgcctg ataaaccaca gactctaaat
     9481 agcggcgaac cagatattcc aacactgcaa gtgcagtcgg ttcgtagcgg tattcccagc
     9541 tgaacggtga tttgggagct gaatcgccaa tcacgttctc accgataggc agcaatactt
     9601 ccattctcgg ttcttgacgc atggtattga caaaacccga atacaccaga tggattctgt
     9661 caatttcatg tttctcatac cgttggaaga gttctgtcaa aggtccgagc agcatttcca
     9721 tttttggggt atcgcccaaa tttacggcac tggcaaccac attcagacca atgctctgac
     9781 acgccatcag acctttactg ccaaggcata cgatttcctc ttcaatacct tgattccgat
     9841 actcttgaac ttgtgccaaa aactttttca gcacgttggc gttcaaaccg ccacacaaac
     9901 ccttatcaga cgtaatcaaa ataaaaccga cacgtctaat ctcccgatga gattccagta
     9961 acggaatacc atgatcggta ttggtttgcg caagatggct catcaccata cgcacttttt
    10021 cggcatacgg acgcgccaaa cgcatccgtt cctgagtctt ccgcatttta gaggttgaca
    10081 ccatctgcat cgctttagtg atcttttggg tattctgaac actgcggatt ttggtgagaa
    10141 tctcttttcc tactgccatt tcagactcct ttcacttcaa gccttatgcc tgataggcgt
    10201 aagaagattt gaaggatttc atggctgctt caagcgtttt ctcgctctcg tcggacattg
    10261 cacctgaagc attgacggct tccaaaactt ccggatgttg ggtacggaca aagctcaaaa
    10321 attcagattc aaaagccaga gctttggcaa ccggaacatc agaatacgaa ccgttgttga
    10381 ttgcccaaag ggtcaaagcc atttcagccg tattcaacgt actgaactgt ttctgtttca
    10441 tcagttcggt tacgacttcg ccatgctcca attgtttgcg cgtagcttca tccaaatcgg
    10501 atgcaaattg cgagaacgcc gccaattcac gatattgtgc caacgccaaa cggataccgc
    10561 cacccagctt tttaatcact ttggtttgtg cagcaccgcc tacgcgggat acggaaatac
    10621 cggcattgat tgcaggacgg ataccggcgt tgaagaggtc ggtttccaag aaaatctgac
    10681 cgtcggtaat cgaaatgacg ttagtcggaa cgaaagcaga tacgtcgccc gcttgggttt
    10741 cgataatcgg caacgcggtc agagaaccgg ttttgccttt tacttcgccg ttggtcaatt
    10801 tctccacttc gtgttcattg acacgtgccg cacgttccaa cagacgggag tgcaggtaga
    10861 acacatcgcc aggataggct tcgcggccgg gcggacggcg caaaagcagg gaaatttgac
    10921 ggtaagccac agcctgtttg gacaaatcgt cataaacaat caaggcatct tcgccacgat
    10981 cgcggaagaa ttcacccatc gtacaaccgg agtaaggtgc gatatattgc aatgccgccg
    11041 cttcagatgc agttgcagca accacaatcg tgtgttccat cgcgccatgc tcttccaatt
    11101 tgcggaccac gttggcaata gaagatgctt tttgaccgat agcgacatag atacagataa
    11161 cacccgtacc tttttggttg acgatggcat ccaatgctac ggccgtttta ccggtttgac
    11221 ggtcgccaat aatcaactca cgctgaccgc gaccgacagg aaccatagag tcaatcgcct
    11281 tcagaccggt ttgcatcggc tggtcaaccg atttgcgcgc aatcacgccc ggtgcgattt
    11341 tttcgatagg ggcggtcaaa gttgtattaa tcgggccttt gccgtcgata ggccgaccca
    11401 atgcatcaac gacgcgtccg accagttcgc gtccgaccgg cacttccaag atacgaccgg
    11461 tacaggtaac cgtgtcgcct tctttaatat gttcgtactc gcccaacact acggcgccga
    11521 cggagtcgcg ctccaggttc atcgccaagc cgaaagtgtt acccgggaat tcgagcatct
    11581 caccttgcat tgcatctgac aaaccataga tgcgaacgat accgtcagtt accgaaatca
    11641 ccgtaccacg ggtacgcact tcggcattta cagacagatt ttcgatcttg gctttaatca
    11701 aatcgctaat ttcagcagga ttaagctgca tgaaaactct cctaattcgt catagtcgtg
    11761 tacaaggcac tcaatttgcc ttgtacagac aaatccaaaa cctgatcacc cacttcaact
    11821 tttatgccgc caatcagctc cggttcgatt tcgacagaga ttttcagctc gctgtcgaaa
    11881 cgcttattca gcatttgcgc caactcgccg acctgtttgt cggtcaacgg ataggcactg
    11941 taaatgacgg cagatttgat atggttgaat gataaggtca agtcttgata ttgagcatat
    12001 acttccggca atatcgacaa acgtttctgc ccggccaaga cgataacaaa gtttttcaac
    12061 tccttgtctt tcaaaccgac caaatcgatg aggatatctg ctttttctga agcattcgtt
    12121 tcaggacggt caatcaatga agccaccttc ccttcctgaa caaccgccgc aagtttttcc
    12181 agtccgccca accaagactc aatttggctt ttttcctgag ccagaccgaa caatgccttt
    12241 gcataaggtc tggcaatcgt tgcgaactct gccataagat tacagctcct gtttcagggt
    12301 atcgagcagt tttgcgtgtt tggaagcatc gacttcgctg cgcaaaatag attcggcacc
    12361 tttgacagcc aacacggcaa cctgctcgcg cagggattcg cgtgcgcgga acaattcctg
    12421 ctccacatcg gcctttgcct gagctgcaat gcgcgccgcc tcggaagaag cctgttcttt
    12481 ggcttcttcg acaattttgg cggcacgttt ttcggcgttg gcaaccattt cggaaacctg
    12541 attacgccct tctgccaaga gttctgcaac ctttttttca gcctgctcga aatcgctttt
    12601 accacgctcg gcagctgcca agccttcggc gacttttgcg gcacgctcat ccaaagcttt
    12661 tgcaatcggc ggccacacga atttcatggt aaaccatacc aaaccgaaaa agacgatgat
    12721 ttgagcgaat aatgttgcat tgatattcac gttacttaac cttcgtattg gggttaatca
    12781 aacaggctgc gcctgtacgg aacggacgaa tccgtcctga ttatgcacct gcaaacgggt
    12841 taacgaaggc gaacagcagt gcaatggcga caccaatcaa gaatgcggca tcaatcaaac
    12901 cggcaatcag gaacagtttg gtttgcagcg gaccgatcag ttcgggctga cgggcagaag
    12961 actccaaata tttagaaccg accattgcga taccgataga ggcacccagt gcgcccaatg
    13021 caacgatcaa accacatgcg atagcaatca aacccatttt aaactcctta aagaaacaaa
    13081 ggttaaacta caaaaacaaa ctacttagga aaatcagtgc gcatcatgtg cctgtccgat
    13141 atagacgaac gccaacgcca tgaaaataaa cgcctgcagg gtaatcacca aaatatggaa
    13201 aatcgcccat gccaaaccgg caataatgtg gaatacaaac agaatcggat ccatgacttc
    13261 gacgctgccg gaagccgccc aagcaccgcc aagcaaggct atcaacaaga ataccaactc
    13321 gcctgcatac atattgccga acaaccgcat accgtgggat acggttttag aaagaaactc
    13381 gaccaaattc aacagaaagt tcgcaggtgc gagttttgca ccgaacggcg cgctgaacaa
    13441 ctcgtgaaac cagccaccca atcctttgat tttgatgttg taatagatac aaatcagcaa
    13501 cacgccgaca gcgagtgcca aagtggtgtt caaatcggca gtcggtacga cgcgcagcag
    13561 ggcgtgatgg ttgccggtaa tgccctgcca taccatcggc agcaaatcga ccggcagcat
    13621 atccatcgcg ttcatcagaa aaatccagac aaacagcgtc agacccaacg gcgcgacggc
    13681 ttttctagac ttttcgttgt gaatgatgct cttacacata tcgtccacaa actcaaacaa
    13741 gatttccacg gcagcctgga aacgtccggg aacgcctgcc gttgcttttt tcgcaccgcg
    13801 ccacaacagg aagctgccga ttacgcccaa caggacggca aaaaagacgg catcaaggtt
    13861 aataaacgaa aaatcagcaa tgtttttcag tccctgaccc tgagtaacat ccgacaaact
    13921 ggtcaagctc tgcaagtggt gcttgatgta gtcggcagcg gtaatggttt cacctgccat
    13981 aatctttcac tctcaacaat actaaaaaaa ccaaatggct gacaccgagc agccccatca
    14041 gaaacggggc gaacaccaac gattgatgcc atacagcaaa tacggcgagc atggacaaca
    14101 gcgacagcac tacttttaaa atctctccga agacgaacat cctgctttgc aggaaagggt
    14161 ttcccctgaa aagttttaaa agtaaaactg caacaaacgt gggaagcagg taggacaaac
    14221 cgccaccgac cgccgaaagg aatccggcaa aaccccatac agcaaaggca actgcggcgc
    14281 atatggacaa tacggcggat tgtaggatga taatctgctt cataaaggga atgtttccgc
    14341 ctcggatttg gggcgcggct aatataattt agaagcctta ttacgtcaag cgacagttaa
    14401 tctttgtgaa acaacgtatc ccaatccgcc gcgctcgccg cctgaataac ggcgacaggt
    14461 gtcattctaa cacacattac atataattac aggatattaa ggagtttgtc cgcaatttct
    14521 ttacattttt aatgttctta cgtgatttgt ttgctttacg tggaaataat aaaaaatcaa
    14581 cgcgaaattg tagcagttta tcggtcggat tgtcggcagt ttggggaatt tgctcaataa
    14641 ataaaaggtc gtctgaaaat attttcagac gaccttttcc gaataaagga ttagcaacgg
    14701 cctgccgctt taagcaaagc attgcattga cttttgcctt tgtgcgttcc gcctcccaaa
    14761 caaattgcat cggaagtggt aacgccgatt gtgctgatta cactggtaac atagcattgg
    14821 ctcacgcgct tacccacagt tgcggtaaag ttgatgcgta tgccttcatt gttgcggttg
    14881 ctgattttta cggcatttgg gctgacgccc aaggcaaacg cggcacgttc ctgaagtttc
    14941 tggtcggaaa cggttacatt attgattgag ccgcaacctg ctaatgccaa cgcaacgaac
    15001 gcagccgaaa cgatgatgcg tgtgttcata atttcctcga aaattaaaaa tgaaaacagg
    15061 aaaacgattc ttacgtgaag cagaaaaaat gtcaatagaa ttatatttcc cacttaaaat
    15121 ctggaaagct attctctata tttcagacgg tatatcccgc aaaattaagg tcggtaatct
    15181 atgcccaatt gcttcaagat atggtcgaag gtttcgggcg tgtcgaaatg caggatgatt
    15241 ttgccttttt tatggttggc ggttttgatt tcggcgttga cacccagttt ttcagtcagc
    15301 aaatcattca ggcggccgat gtcggcggcg gcagtctttt tgggctcggg acgtttgttt
    15361 tgaagggcgg cttggctgcg gcgttcgact tcgcgcaccg accagccgtt tttgacggcc
    15421 ttttgcgcca attcgagctg ttcgacgacg ggcagggtca gcaatgcgcg ggcgtgtccc
    15481 atttcgaggc ggcgttggta aagcatttcc tgcacgggtt cgggcaggct taaaaggcgc
    15541 aggctgttgg aaatcgcgct tcggctttta ccgacggcct gggcgatggt ttcgtgggtc
    15601 agtccgaact cgtcggcaag gcgtttcaag ccttgtgctt cttcgatggg gttgaggttt
    15661 tcgcgctgga ggttttcgat caaacccatt gccaatgcgg tttcgtcgct gatggttttg
    15721 ataacggcgg ggatttcggt caggccggca atctgtgcgg cacgccaacg ccgctccccc
    15781 gcaatcagtt cgtatcggga cagtccgtgt tcgcgcacga tgacgggctg tatcacgcct
    15841 tgcgccttaa tcgaatccgc cagctcctgc aaggcttcgt catcgatttg aacacgcgcc
    15901 tgatagcggc cgggccggat atctttaacc gcaaccgtgg tcaatcggtc gccgctgctg
    15961 ttgtctgcgc cgttggcgag cagcgaatcc aaaccgcgcc ccaatccacc ttttactttt
    16021 gccataccgc cctcccgtgc ctatttcaga taggatgtta aatcggatat tttatcggat
    16081 attgggtgtt gccgacaatt tgtatccgtg tttatcggat ttctgttttt tcactataat
    16141 agctggtttg ccgttgcagg cggttttatg ggaaaggcgg atgatggtac ggcgtttgat
    16201 aatcggcatc agtggggcga gcggtttcca atacggcgtg aaggctttgg aacttttacg
    16261 cgcgcaagat atcgaaacgc accttgtggt atcgaaaggt gcggagatgg cgcgcgcttc
    16321 ggaaacggct tatgcgagag acgaggtata tgccttggcg gacttcgtgc atccgatcgg
    16381 caatatcggg gcgtgcattg ccagcggtac gtttaaaacg gacgggatgc tggtcgcccc
    16441 ctgttcgatg cggacgcttg cctcggtcgc gcacggcttc ggcgacaacc tcttgacgcg
    16501 tgcggcggat gtggttttga aggaaaggcg gcggctggtg ctgatggtgc gcgaaacgcc
    16561 gctgaacctt gcccatttgg acaatatgaa gcgggtaacg gaaatgggcg gcgtggtgtt
    16621 tccccctgtt cctgcgatgt accgcaaacc gcagacggcg gacgacatag tggcgcacag
    16681 tgttgcacac gctttgtcgc tgttcggaat cgatacgccg gattcggcgg aatggcaggg
    16741 aatggcggat taaaggacaa aaatgccgtc tgaacacgga tacggttcag acggcatcat
    16801 tttatacgac tgccttattt ggctgcgcct tcattccatg cggcagggga tttgtagccc
    16861 tcgaagcgtt tgtgcgcgta ggctttgaac gcgtcggagt tataggcctc ggttacgtct
    16921 ttaagccatt ggctgtcttt gtcggcggtt ttgacggcag accagttgac ataggcaaag
    16981 ctcggttctt ggaacagggc ttcggtcagc ttcatgccgc tgcttatggc gtagttgccg
    17041 ttgacgacgg caaaatccac gtcggcgcgg ctacgcggca gttgcgcggc ttcaagctcg
    17101 acgattttga tgtttttcag gttctcggcg atgtccgctt tggatgcggt cagcggattg
    17161 atgccgtctt tgagtttgat ccaacccagt tcgtcgagca tcaccaagac gcgggcgaag
    17221 ttggacgggt cgttgggcgc ggatacggtg ctgccgtctt tgacttcttc cagcgatttc
    17281 agcttgcccg ggtacagtcc caaaggcgcg gtcggcactt ggaagacttc ggtgatgtcc
    17341 agattgtgtt cttttttgaa gtcgtcaaga tagggtttgt gttggaagac gttgatgtcc
    17401 aactcgccct cagccaatgc cagattcggg cgtacatagt cggtaaactc gaccagtttg
    17461 acggtgtagc cttttttctc cagctcgggt tggatgtgtt ctttgaccat atcgccgaag
    17521 tcgccgacgg tcgtgccgaa gacgatttct tttttctccg cgccgttgtc ggcggcggca
    17581 gaagcggatg cggcgggcgc gctgtctttt tgaccgccgc aggcggcgag gatgagcgcg
    17641 agtgcggcgg cggaaagggt tttgaagaag gttttcatat tttctcctga tgttgtggca
    17701 gtttcaaaca aaaatgacgg gcagggagtc ctgccgtccg gattcggcgt tcagacggca
    17761 tttgccgcga acagggggat tttatagcat ttttcggata gcggtggggg ttttggcgtt
    17821 cagacggcat tttgggttca acgtttgtcg agtttccgcg ccaacgcgtt gccggtgctt
    17881 tgaatcagga taacgagcag cacgaggagg gcgacgatga agacgatgac ttcggtttgg
    17941 tagcggtagt agccgtagcg gatggcgagg tcgcccaagc cgccgccgcc tatcatccct
    18001 gccgccgcgc tgtatgacaa aagcccgatg gcaagcacgg taatgctgga aaccatgccc
    18061 gcgcgcgctt cgttcaagag gactttgcag acgatggcaa tcggaggcgc acccatcgcg
    18121 gcggcggctt caatcacgcc tttggggact tcgcgcaggt tttgttccac cagtcgggca
    18181 aaataaaaca atcccgacac gctcaacacc agcgaggcgg caaccggacc gatggtgctg
    18241 ccgacgatgg tgcgggtcgc gggtatcatg gcaatcatca ggatgacgaa ggggaaggcg
    18301 cgcatgaggt tgacgaggtt gtcgagcagg aagttcaccg gcttgttgta atgcagttgg
    18361 cggctggaag ttacgaagag caacacgccc agcaacgtgc cgaaaacgac ggcgaatgtg
    18421 gtggacaagc cgaccatcac gaaggtttcg cccaatgcgc ggacgatttc gcctttcata
    18481 ccgatgatgt tggaaacggc ttgttggaag gtcaggtctg ccatttcagt cctctctgat
    18541 gagttcgcgc ccgatgtcgg atcgggcgtg gatttggttg ccgcgtactt cgacgatttc
    18601 gacgactttt cccttatcca acagggcggc tcggtcgcac agtcgtctga tgacgctcat
    18661 ttcgtgggtt acgatgacga tggttacgtt gaagcgtttg ttgatgtctt ccaaacattc
    18721 caagacgctg cgcgtggtgg cggggtcgag ggcggaagtg ggttcgtcgg cgaggatgac
    18781 ttgcggcttg gatgcgagcg cgcgggcgat gccgacgcgc tgtttttgtc cgccggaaag
    18841 ctgggcggga tagtgaccgg cgcgttcggt cagtccgacg atttcgagac attcggcaac
    18901 gcgcgctttg attttttcag acggccatcc ggcgatttcc aatgggaagg cgacgttgcc
    18961 ggcgacggtg cggttgttca acaggttgaa ctgctgaaac accatgccga tattctgccg
    19021 agcctgacgc aatgcggcgg catcaagcgc ggtcagctct tgtccacaga cgttgacctt
    19081 gcccgtgtcg ggacgttcca acaggttaat cagtcgcaac agggtggatt tgcctgcacc
    19141 cgaataaccc atcagcccga agatttcacc gtcgcggatt tcgaggctgg tcggctcgac
    19201 agcggcaaaa cgggtcttgt cgcgcgtttg gtaatgcttg gaaaccttgt ccaaaataat
    19261 cattgtcttt cccatacaac aaagcccgat gtcggacaca acgggcgcgg aagataaagc
    19321 tgaaattgtc ggaacgcttt agctgttatg cccgcaagct gtgtcaaatc ggcaggttaa
    19381 ttttcgtagg atattatcgg gaaagcgttt ttttgtcaat aaggcaggaa gcgggcaacc
    19441 atttcggaca atgccgtctg aaacaggcaa aggcagcggt tcgcactaaa acggcaaata
    19501 attgaaaaat atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
    19561 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
    19621 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataaatt atgtcggaaa
    19681 cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt cagacggcat
    19741 tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc cgatgtacct
    19801 accctctatg aagcattccc tgccgctgct ggcgacccta gtgcttgccg cgtgttcttc
    19861 gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaagctg ccgacctttc
    19921 ggcaagcgtt cccacccgcc ctgtcgaacc cgaaggaaaa acgctggcag attacggcgg
    19981 ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg ccgcctattt
    20041 agaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc tgaagtcttt
    20101 gggcgcgcgc agacagtgga cgctgtttgc acaggaatat gccaaactca aacctgaagg
    20161 gcgcgcccaa gaagtcgtgt gttatgccga cttgaaacac agcgactata cgcgtgccgc
    20221 cgaactggtt aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt tggaacaggc
    20281 agccgcatcc ggcttgttgg acagcaacaa tgcctggagg cgcgttcgcg gactgttggc
    20341 caacagccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc cgtttgacgg
    20401 cagcacacaa ggttcgcgcg aatacgccct gttgaacgtc atcggcaaag aggcgcgtaa
    20461 atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc ccgaacaacg
    20521 cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg tgcctgccgc
    20581 cttggactat tacggcaaag tttccgaccg ccgccaactg accgacgacc aaatcgagtg
    20641 gtacgcccgc gccgccctgc gcgcccgacg ttgggacgag ctggcttccg ttatctcgca
    20701 tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac gcagccatgc
    20761 tgcgctgggc aacttgcagc aggcagcaaa actctatgaa caggcggcag cgacgggcag
    20821 gaatttttat gccgtactgg caggggaaga gttgggtcgg aaaatcgata cgcgcaacaa
    20881 tgtgcccgat gccgacacaa acagcgtccg ccgcatggcg gaagacggtg cagtcaaacg
    20941 cgcactggta ctgttccaaa acagccaatc tgccggtgat gcaaaaatgc gccgtcaggc
    21001 tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc tgaccgccgc
    21061 gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg aacgcaccga
    21121 ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg taatccgcca
    21181 cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc aggaaagccg
    21241 cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg ttatgcctgc
    21301 caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaatttt acaccgccga
    21361 cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc tgcaaaacaa
    21421 cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc gatggcaggc
    21481 ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg aaacgcgcga
    21541 ctatgtcaaa aaagtgatga ccaatgccgc ctactacgcc gccctcttcg gcgcgccgca
    21601 catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg atgccgtctg
    21661 aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg gcaaaccata
    21721 aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga cacggcacaa
    21781 gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc gcaaggcaca
    21841 gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat gatgggcaga
    21901 tgcaacaaca gaatttatta aaggaaataa aatgcctgca atccgcgtaa aagaaaacga
    21961 accctttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg gcctgctgac
    22021 cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac gcaaaaaagc
    22081 ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc ccaaaatgta
    22141 ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca ccgcaagacc
    22201 tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc catccgacat
    22261 cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa aaccgcgatg
    22321 cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc cgtcaaacag
    22381 tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat cctgaccaaa
    22441 atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg tcaggacttg
    22501 gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca aatgctctcc
    22561 gccggggaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc ggcaggtatg
    22621 gcggatatgg gcaaagtgat gggcgtattg aaaacccgcc tcgccggcaa agccgatatg
    22681 ggcgaagtca acaaaattct gaaagccgta ctgaccgcct gattgtatga atataaggaa
    22741 aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatgtc ccaatatcga
    22801 atcggcaggg gcaacacggt ttcgatacac cgaaacgggt tttgccgata aacggattcc
    22861 gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga cggcatagat
    22921 ttatttgacc aatttcaagc cttttttggc gggccggggc gcggtttcgg cagaggtgtt
    22981 ttcaggcggc gtgtcggggc gatacgcttc caattcaaac cccatacctt cgccggtctc
    23041 ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg cctgtccgct
    23101 gaaacgggcg gaaaaactga tccaatcgtt gtcgatttga aggttttgcg tggcggtcgc
    23161 gccgatgttg agcataattt cgttgtcgcg gacgtactgc atggggacgc gcgtgtgttc
    23221 gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt cgcagagggc
    23281 gcggaggatg tagggtttgg tggaagtggg cataatggtt ccgtgttgta cgccaaaata
    23341 ggaaaatgcc tgcaaaacgg cgggttttgc aagcatttcg gacttatttg cgcatggctt
    23401 tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg gaagatgcgc tcggcgtatt
    23461 tgagcagctg cgcggcactt ttgcccagtt tgacatcgta gtggtcgagc cgccacagca
    23521 gcggagcaag ggcgacatca atcatagaaa aatcttcgcc gaggatgtat ttgcttttgc
    23581 tgaacgaagg ggcgagcatg gtcagaccgt tgccgatggc ttcgcgcgct tttgcctgtt
    23641 ccttgttggt ggcggcgggg ttttctaaca cttggacgtg gttgaacaat tctttttcca
    23701 tacggtacag caccagccgg ccccggccgc gcataacggg atcgccgggc ataagctgcg
    23761 gatgagggaa gcgttcgtcg atgtattcgt tgatgatgtt ggactcgtgc agcaccaaat
    23821 cgcgctcgac cagcacggga acttggttat acggattcat gacggcgagg tcttcgggtt
    23881 tgttgtaaat atcgacgtct ttgatttcaa aatccatacc tttttcgtac aaaacgaagc
    23941 ggcagcggtg gctgaagggg caggtaatgc cggaatagag ggtcatcata ataattgtcg
    24001 ctcctgtgtg atgcctgcaa aacggctgat ttataaaggt ttaatcgcgc aattatacgc
    24061 gatttccggc acttaatcca gaaattcggc tcaatctgtt gtttttaata tattttcccc
    24121 gattttccgt atcagtgcga acttactgtc tttgttgcgc gggcgcgcac cctgccagac
    24181 cgtctgccag ccttgcgacg catccgcatt ttggggcaag cggacgatac ggtagcggca
    24241 ttgtacatcg ccgacgcggt gcggcaatgt gccgtactgc gtccaaacaa tccgcgtgtg
    24301 caggtcgccg ccgcctatgt cgatacactc gatgccgtct gaaagctccc gttttaattc
    24361 cggggaaagc gatgcctcca tactccggac gaccggcgcg tggcttttcg ccgcgtccag
    24421 ccacggcagg aacagcgtca tcagcaaagc ccaggtcagg gtaacgcctg ccgcccagtt
    24481 ggtaaccgcc tgcctgccgc gtatgttttt ccgggtaatc gcccacagcc acaagggtgt
    24541 gaacagtacg gcaaccgcca tcggaatggg atcgatatca ggaacataat acgggctgaa
    24601 gtaggcggcg cgttcggcaa gcttggcggg ccagccgtaa ttcatagcga aaaaacccgt
    24661 ccacaggaac acggcaaaca gtccgaacgc cataatgccg aaccagttga caaacgccgc
    24721 cgcgccgcgc ctcaggctgt ccagttgcgc cgcgccgaac agggcaagcg gcggcagcag
    24781 ccagacgagg ttgtcctgaa aacgctgcgg attggcggcg agcagcacca aaacggcaag
    24841 catccacaca acgcccaaaa tcccccagtc ggtcgaaaac aggcgcgtgc ggcaaaccgt
    24901 ccaaaccgcc agcggcagcg cgggcagtgc aaaccaaagc aggtttttca gatagtaaaa
    24961 caaactgaat gccgtctgaa cgtgccgcac gccgccgaac gtaccgaaaa cgtgatcgtc
    25021 gagccattgc gcgaacagcg cgggctgcgt ttttgccaag agcagcgggt aaacggtcat
    25081 cagcggcagg gcaaaggcaa gcgaggcgac tgccgtcaac atcaaacgcc tgctttgcca
    25141 cggacggaaa aacatcagca ctggcagggg cagcatcagg gcaaatgctg ccggataagc
    25201 tgctgccaac gacatcagcg tccaacccgt accgagcaga aaagaggcgg caatcacacg
    25261 gcggcacgcc agcgagtagc cgtgcagcac cagtccggcg gcggcaaagg cggcggcagc
    25321 gggattgaga aagtgtacgg tcggaatcag cccgatacag ccgataagga ttaaaacaac
    25381 gctgcgcccg tggtgtctgc ccaaaaagtt gaaaccggca aagccgcagg aagtcagtcc
    25441 gacaacggcg aaaaacacgc ctgcaaagcg tgcggcatcg tatgagtcgg cagcccacgg
    25501 cgacagcaaa tgtttgaacg cggcggcaac ccaaagatac acgggcggta tgccgaaatc
    25561 ggtttgaccg aacagatggg caaccaaggg ggtggggctg cctgccagtg cttcgacggc
    25621 ggtatagacg gcaggttcgg caggattcca taaatcatgt gaaaatacac ccggccacaa
    25681 ccaggcaaac gccatcaaca gcagcagcca cggcttttcg tgggttttgg cgggcgggcg
    25741 ggcatcgggc ggggtatagg tcagcataag cgtgagaaaa aatggacgga ttgccaagtg
    25801 tagcaaatat tcgcacaaag gtcgtgcaga gactgcttca gacggcatca gacacaaaaa
    25861 gaccggcaac aaaaaagact gcacatggca gtctttgcag atactatctt tttcataata
    25921 ttttttccta gcccaacaca gtccatagca tatcaaaact gttgttttca accaggatat
    25981 atatccccaa tcctaaataa acaacagcaa caaaccatct gctatatttt tccaaagttt
    26041 ctccaacaga agggacttgt gctaattttt gggcagaaaa aaccaagaga taaatcatga
    26101 ctagaaaggt aagtaaagcc actatcaaat tcgctaaatt taaggtagta aaatatggga
    26161 caaagacacc aatattgtca gcaccacaac ttgcaaaagt aatcatagcg actagaaaaa
    26221 tcaggttttt attatctttg cgcaaaccct ctttggcaat agcctctcca tcagaatctc
    26281 ctaacagcaa aactttgatg cctaggaaaa ttggaatcaa gccgagcaaa cctaaaatct
    26341 ctttactagg aatataatct aagacaaatg caaaaagcaa acttagcaat atcaggctaa
    26401 cagagcctag aaattgtcct aaatagatgt taatgatgtc ttttctgctt tttcttttgg
    26461 caaaaaataa cattaggata ataagtaagt ctacggctgt cccagaatac aggattattg
    26521 aagtaacaac attttgaatc ataaaacatc tcattcaaat atatttttaa atgtattcaa
    26581 acattaaacc ttgtagatgt caacttcaac cccgtcaaa
//
LOCUS       DAWFIT010000018        26465 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFIT010000018 DAWFIT010000000
VERSION     DAWFIT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.018"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(242..844)
                     /locus_tag="WGQ85_000745"
     CDS             complement(242..844)
                     /locus_tag="WGQ85_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0750220.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGQ85_000746"
     CDS             complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGQ85_000746"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0750221.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            2307..2624
                     /locus_tag="WGQ85_000747"
     CDS             2307..2624
                     /locus_tag="WGQ85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0750222.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(2676..3287)
                     /locus_tag="WGQ85_000748"
     CDS             complement(2676..3287)
                     /locus_tag="WGQ85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0750223.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIVFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(3350..4285)
                     /locus_tag="WGQ85_000749"
     CDS             complement(3350..4285)
                     /locus_tag="WGQ85_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0750224.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEAIPAQNLSRFVNRKLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(4296..5045)
                     /locus_tag="WGQ85_000750"
     CDS             complement(4296..5045)
                     /locus_tag="WGQ85_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0750225.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGQ85_000751"
     CDS             complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGQ85_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0750226.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            complement(6277..6912)
                     /locus_tag="WGQ85_000752"
     CDS             complement(6277..6912)
                     /locus_tag="WGQ85_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0750227.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            7027..7707
                     /gene="tsaA"
                     /locus_tag="WGQ85_000753"
     CDS             7027..7707
                     /gene="tsaA"
                     /locus_tag="WGQ85_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0750228.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            7776..7895
                     /locus_tag="WGQ85_000754"
                     /pseudo
     CDS             7776..7895
                     /locus_tag="WGQ85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGQ85_000755"
     CDS             complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGQ85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0750229.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            9413..12007
                     /gene="mutS"
                     /locus_tag="WGQ85_000756"
     CDS             9413..12007
                     /gene="mutS"
                     /locus_tag="WGQ85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0750230.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTTLPQLQKAAKAAAALDVLSTFSALAKERNFARPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPFDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(12131..12622)
                     /locus_tag="WGQ85_000757"
     CDS             complement(12131..12622)
                     /locus_tag="WGQ85_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0750231.1"
                     /translation="MNSLFVDNTVFITRLKAVHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDGELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGQHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(12619..13020)
                     /locus_tag="WGQ85_000758"
     CDS             complement(12619..13020)
                     /locus_tag="WGQ85_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750232.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGERRRRGIDR"
     gene            complement(13041..14435)
                     /locus_tag="WGQ85_000759"
     CDS             complement(13041..14435)
                     /locus_tag="WGQ85_000759"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0750233.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(14551..15207)
                     /locus_tag="WGQ85_000760"
     CDS             complement(14551..15207)
                     /locus_tag="WGQ85_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0750234.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(15204..15557)
                     /gene="arsC"
                     /locus_tag="WGQ85_000761"
     CDS             complement(15204..15557)
                     /gene="arsC"
                     /locus_tag="WGQ85_000761"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0750235.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            15681..16163
                     /locus_tag="WGQ85_000762"
     CDS             15681..16163
                     /locus_tag="WGQ85_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0750236.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            16171..16362
                     /locus_tag="WGQ85_000763"
     CDS             16171..16362
                     /locus_tag="WGQ85_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750237.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            16359..17009
                     /gene="ftsE"
                     /locus_tag="WGQ85_000764"
     CDS             16359..17009
                     /gene="ftsE"
                     /locus_tag="WGQ85_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0750238.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            17006..17923
                     /gene="ftsX"
                     /locus_tag="WGQ85_000765"
     CDS             17006..17923
                     /gene="ftsX"
                     /locus_tag="WGQ85_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0750239.1"
                     /translation="MSIIHYASLHIESARTALKQLLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDDNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            18024..18272
                     /locus_tag="WGQ85_000766"
     CDS             18024..18272
                     /locus_tag="WGQ85_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0750240.1"
                     /translation="MLTPEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(18340..19518)
                     /locus_tag="WGQ85_000767"
     CDS             complement(18340..19518)
                     /locus_tag="WGQ85_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0750241.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMADKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(19599..20852)
                     /gene="murA"
                     /locus_tag="WGQ85_000768"
     CDS             complement(19599..20852)
                     /gene="murA"
                     /locus_tag="WGQ85_000768"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0750242.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            complement(21072..21147)
                     /locus_tag="WGQ85_000769"
     tRNA            complement(21072..21147)
                     /locus_tag="WGQ85_000769"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21112..21114),aa:Lys,seq:ttt)
     gene            complement(21216..22190)
                     /locus_tag="WGQ85_000770"
     CDS             complement(21216..22190)
                     /locus_tag="WGQ85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0750243.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            complement(22223..22672)
                     /locus_tag="WGQ85_000771"
     CDS             complement(22223..22672)
                     /locus_tag="WGQ85_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0750244.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            complement(22713..23984)
                     /gene="waaA"
                     /locus_tag="WGQ85_000772"
     CDS             complement(22713..23984)
                     /gene="waaA"
                     /locus_tag="WGQ85_000772"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0750245.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHCPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLS
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(24047..25495)
                     /gene="gnd"
                     /locus_tag="WGQ85_000773"
     CDS             complement(24047..25495)
                     /gene="gnd"
                     /locus_tag="WGQ85_000773"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0750246.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FTAWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGTDPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            complement(25575..25996)
                     /locus_tag="WGQ85_000774"
                     /pseudo
     CDS             complement(25575..25996)
                     /locus_tag="WGQ85_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6609 a         7427 c         6064 g         6365 t
ORIGIN      
        1 aatcatcttc ggcgttttca tttttatgga ttaaaacaac acgggaaaaa tctgttttca
       61 gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg tcctcaaaat
      121 taaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca tgtagagaaa
      181 tgctatccga aaaatttttt ctttgtgtat gcaaaaaaag ttttcattca agtacccata
      241 tctaacgcaa acgtttacct gtttccccgt caataatctg actcggcgat ttctgcctgc
      301 cgattctccc accaacaatc cacacatcgc gtccgaattg ccttctgact tccctctccg
      361 tccgacacgc gcgtttgcct gcgcggttgc acgaagtcga gaccaaaggc gtttgcaaag
      421 cctgacacaa gcggcgcgca cctacatggg cgggaaccct gaccgccaac ttgctgcgct
      481 gtttcccgcg caactcaggt aagacacagg atttggcgga taataggaac gttttagggg
      541 cgggccattc ttttctaagc atatcctgca gattttcaga cggcatttga agtaaaggct
      601 gcaattgctc aaattgattc ccgatgacaa tcataccctt gtgttgcggt ctttttttca
      661 aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag ccataacaag
      721 attcggtcgg ataagcgacc aaaccgcctt ttttcaaata aacgcttaac ttacgttgcg
      781 ctgatgctgc gataattctc ggaaataaca taatataaaa taccgtctga agcacatcag
      841 tcatacttgg cttcagacgg catcatcctc tttctaatta acggttaatc gctttatcgg
      901 caatgtcttt acggtattgc atcccgtcga aactgatttt ttccaacgcg ccatatgcct
      961 tagctttcgc ttgcgccaca ttatcgccca atcccacaac acacaatacg cgtccgccgt
     1021 tggtcaatac gtcacctttc tcgtttgccg ttgtacctgc atggaaaact ttgcctactt
     1081 ggttggcagc atccagaccg gaaataacat cgcctttttt gggcgtttcg gggtaatttt
     1141 gcgccgccag taccacgccc acggcagttt gcgggctcca ttccgcggtt acgctatcga
     1201 gtttgccgtc tattgccgct tcaaccaaat ccgataagtc gctgttcagt cggctcataa
     1261 tcggctgggt ttcaggatcg ccgaaacggc agttaaactc aatcgtatgg ggtgcaccgc
     1321 tttgatcaat catcaaacct gcgtacagga aaccggtgaa ctcatgcccc tccgctttca
     1381 tccctgctac ggtcggcaaa ataatttcat tcatcgcgcg ttcgtacaca acaggcgtta
     1441 ccacaggcgc agggctgtac gcacccatac cgcccgtatt cagacctttg tcgccgtcta
     1501 aaagacgctt gtggtcttgg ctggttgcca taggcagtac attattgcca tcaaccatga
     1561 cgataaaact cgcttcttcg ccttgcagga aatcttcaat cacaatacgc gcgccggcat
     1621 tgcccatttt gttgtccagc agcatatcat caatcgcagc atgcgcttca tccaaagtca
     1681 tcgccacaat cacgccttta cctgccgcca aaccatcggc tttgataacg ataggcgcac
     1741 ctttctgatt gacgtaatca tgtgcggcat cggcgttttc aaaggtttga tattgcgcgg
     1801 tcggaatatt gtatttcgcc ataaatgctt tggcgaaatc tttggaactt tccaactgcg
     1861 ccgcatattg tgtcggaccg aatattttta gtcctgcagc acggaaatca tccacaacac
     1921 ctgccgccaa aggcgcttca gggccgacga cggtaaaaac aatattttct ttacgacaga
     1981 attcaatcaa atcctgatgc gcagtcaagt cgatgttttg cagcttgggt tcaatcgctg
     2041 taccggcatt accagacgca acaaatactg tttccacttt aggcgactgc gccaatttcc
     2101 aagccagcgc gtgttcgcga ccgccattac cgataaccag cagtttcatg ccatctcctt
     2161 gacaaatatg tacttttaac gaaaactcga tacaaaggga cttttatccc atctgaagaa
     2221 attttagtag aatcaaacaa aagaccgctt cattccactc tgcaacctat tcaacttatt
     2281 cataaattaa aaaaggacaa gcaaccatgc aaaaacgtat tgatgaaatc caaagcaaat
     2341 accgcgaatg gtgtcattta ctaccgcaat tggaagaaga catccgccgt tggaaacatg
     2401 ccgtcgcttt gattcgcgat atggacaatt tttataccca cgaatatcag gcttgtcatc
     2461 gggctattga agatggagcg gaggtggatt tgcgcacaga aggcgaatac agcattatga
     2521 gtgaagatgc actatggaac gcgctgggcg aattccatca actggcttgg ttatatttgc
     2581 gctccagcgt cgatgcctta gacaaatata cacaagaaga ttagtcagcg aagaggtcgt
     2641 ctgaaatacc atcacaaagc atttcagacg acctttcatt caaaaggctt ttccgtattt
     2701 acttcaatct gccgtgtact cttccaagcc gcaacacagg cctcataatt taccaacgac
     2761 aaactgaccg tcaatcggca atccaactgc aaatcccgct ccaatacatc cgcctgatat
     2821 tgtttagcaa tgcgtatcgc ttcattcaaa aacggatatt cacatttcag ccaaacagtt
     2881 ttttcaatat tcttttcaac tacttctgca actgccaacg cttgagccgt cgcctctttg
     2941 tacgcatgta tcagacctgg aacacctaac aaagtaccac cgaaatagcg gacgaccacg
     3001 accaaaacgt cggtaatacc caccgaatca atctgtccca aaattggtcg tccagcactt
     3061 cctgatggct ctccatcatc gttggcacga aattgcacac catccacacc caaacgatag
     3121 gcatagcacc agtgtcgtgc tttatgatgc tcttccttta acggatcgag gtattttttc
     3181 acatcagcca atgtccgaat cggataggca aatacaataa aacggctgcc tttatcttta
     3241 aactcagcct gcgtcgagga ggtaatggtt ttataagtcg taatcatgct gaaatgtttt
     3301 cagacgacct cattaataac aaggtcgtct gaacgtttca cgtgaaacat taatttttca
     3361 atacttctgt taattgtgga acgatttcaa ataaatcgcc aaccaatccg taatcggcta
     3421 cattgaaaat cggcgcatca gcatctttat tgattgcaac aatcacctta ctgtcttgca
     3481 taccggcaac gtgttgaatc gcacctgaaa taccgattgc aaaatagagt tgcggcgcaa
     3541 ctactttacc ggtttgtccg acttgagcat cgtttggcgc atattcggca tcaaccgccg
     3601 cacgggatgc accgattgcc gcacctaaaa catccgccaa cggtgtcagc acttcattga
     3661 atttttccgc actacccaac gcacgaccac cggaaacaat cacttttgcc tgagtcagtt
     3721 caggacgatc ggaatgagaa agcttgcggt tcacaaaacg actcaggttt tgagcaggga
     3781 ttgcttcaac attaattacc tcggcatgac caccttgtac aggagctgca tcaaaagccg
     3841 tcgcacggaa ggtcagcacc aatttttccg aatcaacttg cacggtttca aatgcgttcc
     3901 ctgcataaat ggggcgcaca aaaatcgtgt tatccacaat ctcggtcaaa tcagaaattt
     3961 gcggtacatc taataaggct gctacgcgag gcaaaaggtt tttaccgaat gtagttgccg
     4021 ttgctgcaac atagcggtaa tccgctgcca atttaacaac cagcggagcc aactcttcag
     4081 ccaaaccttc ggcataatgg gcagcatctg caaccaaaac tttttccaca cccgccactt
     4141 gttttgcgga ctctactacg gcagatgcac cgcttccggc aaccaataaa tcgactttac
     4201 ccagcttggc ggcagcggta acagcatgca aagtggtagg attcaactgt ttgttgtcgt
     4261 gttcgacaat aatcaatacg ctcatttcag cctcctcaaa tcactttggc ttcgtttttc
     4321 aatttttcaa ccaattcggc aacgcttgct acttttacgc ctgcctgacg cgccttaggt
     4381 tcggcaaatt tcaccgtttt cagacgaggt aaaatatcgg tagccaaatc ggcaggcgcc
     4441 agtttttcca gaggtttttt ctttgccgcc ataatattgg ggagtttgac aaagcgcggc
     4501 tcgttcaaac gcaaatcagc actgataaca gcaggcagtt ttaatgctat ggtttcttcg
     4561 ccgccatcaa tttcgcgcac aatctgtact tcaccacctt caatgtgtac ttttgaagca
     4621 aacgtacctt gcgccgcatt cagcaaagct gccagcattt gcgccacttg attggcatca
     4681 tcatcaatcg cttgtttgcc caaaaagaaa atttgcggat tttctttgtc cgcaacggct
     4741 ttcagcaact tagcaacggc cagagactcc agtttagtat cggtttcaac atgaatggca
     4801 cggtcggcac ccatcgccaa agctgtacgc aaggtttctt cgcatttttt ctcacccaaa
     4861 gaaaccgcta cgatttcgct tacttttccg gcttctttca aacggacagc ttcttccaca
     4921 gcgatttcgt caaacggatt catcgacatt ttgacattgc cgatatccac atccgaacca
     4981 tcggctttta cacgaacttt gacgttgtag tccactacgc gctttactgc gaccagtgct
     5041 ttcattgaac cctcctaaaa agaacgctgc tttcaccatc cagcgaaacc aaaccttctt
     5101 ccctataaaa ccaaatccgt tttccttaaa aacgaattca ttcaaaaatc tttcggataa
     5161 tgcttgccga ttataccatt tttaaagcat ttactcagac tagcggatat acattcctgt
     5221 atctaataaa ttggaaaata tcatgccgcc atatcagttt tagacgaccc tttagccttt
     5281 atctgctgca acacaatcca tcagcgcttg ataaaccaaa tctgcggtcg gaatctgccc
     5341 gatattgccc aaattttttg caattggcga aacctgaacg cctgttttaa tcggatcggt
     5401 atcggtataa atgccgacca caggtttttc caaggcgttt gccaaatgca gcaaaccggt
     5461 atccacgcca acaattccga ccgcgtgttt cagcagatac gctgcctgca ataaattcat
     5521 tttatcgcac acaatagcaa acggcagtcc gtctgcaatt tgtttggcac gcgttttttc
     5581 atcttcattt ccccaaggca ggtaaatatt gcattgctgt tcttcattca acttttgcag
     5641 caacgaccgc cagttttcca caggccataa cttactgtcc cgactggtcg catgcaaagc
     5701 cgcataatac ggctgcgcta aatttttcag acggcctgcc tcaggaacag tcaagccaaa
     5761 tacctgcgtt tcaggcatcg catacccaaa tacttgggcg aacagctcac gattgcgcca
     5821 aacagcattt ttccctttcg gtacagcgta tgttttcgca tacgccagcg cagccagtcc
     5881 ctcgcgcgcg ctatttttat ccaaaccata aataggggat ttcgccattt tagcgaaaca
     5941 cgcgctttta atcagacctt gactgtccaa tacaaaatta aatgcttcct gccgcaaagt
     6001 ctgtttcaga tgacccattt cccgccaagt ttcagcctga aagagatgtt tgcgccattg
     6061 ccgccatttc atcacatgga ttttttttac aaacggatgc aggcgcgcaa tatctgcaaa
     6121 tccagcctca catagccaat gcagttctac atcaggacat tgtcgcgcca aatcttcgat
     6181 tgcgggcaaa gtgtgaatta aatcgcccat actagacaag cggacaagca aaattttcat
     6241 atttaagaag tatgtttcac gtgaaacgat tttaacttat tgattattaa tatatttttt
     6301 aatttcttcg gcgtttttta agatgacagc accggcagaa cgcatttcct gccatgccgt
     6361 ttcaatggta tccggcgcaa taccccgaca agccgcttca ttgacgataa cctgccaatt
     6421 gctacctttg agcaactgca aaaccgttgt tttaacacaa taatccgtag ccaaaccgcc
     6481 gacaatgacc atatccgtat tttgacaacg cagccactca atcaatccag tgctcagttt
     6541 ttcctcaata tcgtgaaaac acgcgccgta aggatgcaat tcaggatcaa cacctttcca
     6601 aacgcaataa tcgtattctt tggcagaagg cagtccgtcc aataattcat agccgcgcgt
     6661 accgaccatc gcatgagcca cccaagtcaa atccgcatca ggcaaacctg tcggcttcaa
     6721 catatcaaca gggttatcca caagccattt cgctaccata tgatgcgcat ctttcgtcat
     6781 cacgcgcaaa tccgccaaag cggcttgggc attcaattct tcgacaatca aatgcccctc
     6841 gtttacaggc agctctttag gacacaaagg cgtaaaagtt ttttgtgcat caacatcaat
     6901 ggaaacaatc atctcatcat ttcaacgcaa ttaaaatgcc ctgtattata acaaattact
     6961 gcccaaaagc ggtaaaacag gctgtgataa gataaggttt ttccgaaaaa cttatccaca
     7021 atcctcatga cttacaccat cgtccctatc gccaccgccc gatcgcccta caaacagaaa
     7081 ttcggcatcg cccgccagcc cggtttagtc tccgccgcag aagtatgcat cgagctgaat
     7141 cccgaattta ccgcagacag cgtgcgcggg ctggaagatt tcgattatgt gtggataagt
     7201 tttatttttc acggcgtatt ggatgaaggc tgggcacaaa tggtgcgccc gccacggctc
     7261 ggcggcaaac aaaaaatggg cgtattcgcc acgcgcagcc cccaccgccc caaccatctc
     7321 ggactatcac tcttaaaact caaccgcatc gaaaccggca aacccgtccg cctttattgc
     7381 agcggcgcag acctgctcga cggcacgccg attgtggaca tcaagcctta tatccccttt
     7441 gtcgaatcca aacccgatgc cgcatctggt ttcgtcagcg gcaaacccgt agagttggaa
     7501 gtcgtttggc aggaaaacat cggcgcggaa aatttatctg caaacaccaa aaaccttatc
     7561 agccaaagca ttgcccaaga tccgcgcccc gcctatcaga atattcccga acggatttat
     7621 gtgatgaata ttgcggatta cgaaatcaga tttcaaatcg aggaaaaccg tgcaaccgtt
     7681 attgatcttt ccccaacccc gctttaaatc gggcaaaaat ccggttttgc cgcatagcag
     7741 ttgaacaaac ggctgttgtt tgttcgccat aagccgcaat atcaagttat agcggattaa
     7801 atttaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7861 taacgccgta ctggtttaaa tttaatccac tatacagata aacaatgccg tctgaacgca
     7921 atgtgttcag acggcattta cttatccaca ggtttgttca agccttagat tttgcctgcg
     7981 aagtattcca aagtgcggac gagttggcag gtgtaggaca tttcgttgtc gtaccaggca
     8041 acggttttca ccagttgttt gccgcccacg gtcatcacgc gggtttgggt tgcgtcaaag
     8101 agcgagccgt attcgatgcc gacaacgtcg gaagaaacga tttgatcttc gttgtagccg
     8161 taagattcgc tggcggcggc tttcatcgcg gcgttgattt cttctttggt tacagggcgt
     8221 tcgaggatgg aaaccaattc ggtcagcgag ccggtggcaa cagggacgcg ttgggcggag
     8281 ccgtcgagtt tgccgttcaa ttcggggata accagaccga tggccttggc ggcaccggtg
     8341 ctgttgggca cgatgttgag cgcggcggct cgggcgcggc gcaaatcgcc tttgcggtgc
     8401 ggcgcgtcaa gggtgttttg gtcgccggtg taggcgtgga tggtggtcat cagaccttcg
     8461 actacgccga actctttttg caggactgcc gccatcgggg caaggcagtt ggtggtgcag
     8521 gaagcagcgg agataacggt ttcgctgccg tccaaaatgt tttggttcac gccatatacg
     8581 acggttttca catcattgcc gctaggtgcg gaaatcacga ctttgcgcgc gccggcacgg
     8641 atgtgggctt cggctttggt tttattggta aagaagccgg tacattcgag gacgacatcc
     8701 acacccaact cgccccaagg caattcttcg ggattcggat tggcgaaaac tttgatttct
     8761 ttgccgttta ccacgatggc atcgtctttt aattcggcag taccttggaa acggccttgc
     8821 gtgctgtcgt atttgaaaag gtgcagcagc atttcggcag gggtcaggtc gttgacggcg
     8881 acgacttcga tgtcgtgggc tttttcaatt tgacgcaatg cgaggcggcc gatgcggccg
     8941 aaaccgttaa tcgctacttt aatgctcatg tatatactcc aagctgtgaa acgaaatttc
     9001 aatacctgta ttgtattctg aaataaagtt acattccact attacatcta actacttgat
     9061 gcttatttga tatagatgaa ttttactgtt tgcacagatt tccaaaactt ttaccatcaa
     9121 tatttgaatt taaaatttta atgatgattt tgatgattgc caacctgctt gtgcgtaagt
     9181 agcaaatatc caatattttc attacctttt tgtcaaataa gtttgagttt aagacttgct
     9241 gtataagaca gataagcgtg gatgtttttt gacttaataa tatttctgtg gataactttg
     9301 ctgttttcct agttgtctcc acaaccttat tgacaggctt acggtcagtc tcattccgtc
     9361 gaagacaaaa ccttttgcta caataccgtt ttcctaatga taaggcagcc ccatgtccaa
     9421 atccgccgtt tccccaatga tgcagcaata cctcggcatc aaagcgcaac ataccgacaa
     9481 actggtgttt taccgtatgg gcgattttta cgagatgttt ttcgacgatg cggtagaagc
     9541 ggcaaaactt ttggatatta ccctgaccac gcgcggacag gtggatggcg agccggtcaa
     9601 aatggcaggc gtgccgtttc acgccgccga acaatatctg gcgcgcctgg tcaagttggg
     9661 caaaagcgtg gcgatttgcg aacaggtcgg cgaagtcggc gcgggcaaag ggcctgtgga
     9721 gcgcaaagtc gtgcgcatcg taacgcccgg cacgctgacc gattccgcat tgctggaaga
     9781 caaggaaacc aaccgcatcg ttgccgtgtc ccccgacaaa aaatacatcg gtttggcgtg
     9841 ggcatcgctg caaagcggcg aattcaaaac caagctgaca actgtggata aattggacga
     9901 cgaactggcg cgcctgcagg cggcggaaat tctgttgcct gacagtaaaa acgcaccgca
     9961 acttcagacg gcatcgggtg ttacgcgcct gaacgcgtgg cagtttgccg ccgacgcggg
    10021 ggaaaaactg ctgacggaat atttcggctg ccaggatttg cgcggcttcg gtttggacgg
    10081 caaagaacac gccgttgcga ttggcgcggc aggtgcactg ttgaactata tccgtctgac
    10141 gcaaaacctg atgccgcaac atttggacgg cctgtcgctc gaaaccgaca gccaatatat
    10201 cggtatggat gccgccacgc gccgcaatct cgaaatcacg caaaccctct ccggcaaaaa
    10261 atcgccgacc ctgatgtcca cgctcgacct ttgcgctacc catatgggca gccgcctctt
    10321 ggctctctgg ctgcaccacc ctttacgcaa ccgcgcccac atccgagcgc gccaagaagc
    10381 cgttgccgcg ctggaaagcc aatacaaacc cctccagtgc cgtctgaaaa gcattgccga
    10441 catcgaacgc atcgccgccc gtattgccgt gggtaacgcc cgcccgcgcg atttggcatc
    10501 tttacgcgac agtctgttcg agttggcaca aatcgacttg tccgcaaacg gcagcagcct
    10561 gttggaaacc ctcaaagccg ttttcccgga aaacctatcc acagccgaac agctccgcca
    10621 agccattttg cccgaacctt ccgtctggct gaaagacggc aatgtcatca accacggttt
    10681 tcatcccgaa ctggacgaat tgcgccgcat tcaaaaccat ggcgacgaat ttttgctgga
    10741 tttggaagcc aaggaacgcg aacgtaccgg tttgtccaca ctcaaagtcg agttcaaccg
    10801 cgttcacggc ttttacattg aattgtccaa aacccaagcc gaacaagcac ctgccgacta
    10861 ccaacgccgg caaaccctca aaaacgccga acgcttcatc acgccggagt tgaaagcctt
    10921 tgaagacaaa gtgctgactg ctcaagagca agccctcgcc ttagaaaaac aactctttga
    10981 cggcgtattg aaaaaccttc agacgacatt gccgcagctt caaaaagccg ccaaagccgc
    11041 cgccgcgctg gacgtattgt ccacattttc agccttggca aaagagcgga acttcgcccg
    11101 ccccgagttt gccgactatc cggttatcca catcgaaaac ggccgccatc ccgttgtcga
    11161 acagcaggta cgccacttca ccgccaacca caccgacctt gaccacaaac accgcctcat
    11221 gctgctcacc ggccccaata tgggcggcaa atccacctac atgcgccaag tcgcgctgat
    11281 tgttttattg gcacacaccg gctgttttgt gcctgccgat gccgccacaa tcgggccctt
    11341 cgatcaaatc ttcacccgca tcggcgcatc ggacgacctc gcctccaacc gctccacttt
    11401 catggtcgaa atgagcgaaa ccgcctacat cctgcatcac gccaccgaac aaagccttgt
    11461 tttaatggac gaagtcggac gtggtacttc cactttcgac ggcctcgccc tcgcgcacgc
    11521 cgttgctgaa cacctgctgc aaaaaaacaa atccttcagc ctgtttgcca cccactattt
    11581 cgagctgacc tacctgcccg aagcccacgc cgccgccgtc aatatgcacc tttccgcgct
    11641 cgaacaggga caggacatcg ttttcctgca ccaaatccaa ccgggtcccg ccggtaaaag
    11701 ctacggcatt gccgtcgcca aactcgccgg cctgcctgta cgcgcattga aatccgccca
    11761 aaagcatttg aacgaactgg aaaaccaagc cgccgcgaac cgtccccaac tggatatttt
    11821 cagtaccatg ccgtctgaaa aaggagatga accgaatgtg gacagctttg tggacaaagc
    11881 agaggaaaaa cattttgaag gtatattggc agcagccctg gagaaactcg atcccgacag
    11941 cctgaccccg cgcgaagcat tgtcagaact gtaccgtctg aaagatttgt gcaaatccgt
    12001 atcttaattt ccgttgtcgg aacagcatca aaccatatgg aaaaatctgt ggataaacat
    12061 tatctgacag gaaatttcca aatataaaaa atgccgtccg aacagctcag acggcatctg
    12121 tccattcggc ttaaacctta tccacatcca aacgcataac cgtaacccat tcaccgttat
    12181 ggaaatgttg cccgacaacc acccagccga atgattcata aaatatttgc acatcaggcg
    12241 tataaagata caagaacttt atccccagcg aacgcgctgc gcctatgcag tgggcgacca
    12301 gcctcctgcc aatgcctttt ccgcgatatt caggtaaaac aaagacatca cccaaccaat
    12361 attcatactg tggaaaactt tccatatcat gccgcttgac cgtagccgaa cccaacagga
    12421 ttccggagtc atccacagcc gcaaatgcca gtggcagttc gccatccttc aaacacctgc
    12481 cgtaataggc atgaatctta tccacagaag accacggttc aaatccgtgc cactcctcaa
    12541 acaacgcctg aaccaacctg ccgatatgca cggctttcag ccgtgtaatg aaaacagtat
    12601 tgtctacaaa gagggaattc atcggtcaat tccccgacgc cttcgttccc ctgcgccgta
    12661 aaccgcattc caagcatggt ccaaacgcac tccgatttgc ctcagctctt cagcctgccg
    12721 ggctttttgc gccattgctg caggaatttc cgcttccaaa cgggcgatgt ctgcctgagc
    12781 cgcctgcaaa cgccggcgcg catcttccaa atccgactgc atcccgatga tttttccgtc
    12841 cagattgttt tgcttttgca ataaggcacg gtaaccggat tggatactga gcaaattgtc
    12901 ttcagcatcc cctgcccata cgcttgtaga aaaaacaaac atcagaaaat acaatatttt
    12961 tttcatgttc aacttccgtt tgaatgccgt ctgaagccgc attccgacat cagacggcat
    13021 tacccgaacc tgtggataac ttaagcgcgg atgcgtttca acacttcttc tttgccgatt
    13081 aatgccaaca cagcatcgac gctgggggtt ttcgccgtac cgcagacggc aaggcgcagg
    13141 ggcatgccga gtttgcccat tttaatgcct tcttcgtcgc agaagggttt gaagaggtcg
    13201 tggatggctt cggcattcca gtcttccagc ccttcgaggc gttcggcaaa gcgcagcata
    13261 cgggcggcgg cttcatcgtc ccagtgtttc tgcacgtccg cttcagtagg cgtttgtttg
    13321 acgtagaagt agaggcactc gtcggcaagt gtgttcaggt cttgggcgcg gtctttgacc
    13381 agtgccaaca cgtcttccaa tgcaggtttt tcagtttcat ggatatcgcg cagagcgaga
    13441 cgcggtttga cgagttcggc gagtttgccg ttgggcgtga ttttgatgtg ttcgccgtta
    13501 atccagtaga gtttcttcaa atccatgcgg cttggagatg gagaaacgtc tttcaaatca
    13561 aaccattcga taaattgttc catcgtgaag aactcatcgt cgccgtgcgc ccagcccaaa
    13621 cgtgccagat agttgagcat ggcttcgggc aggatgccca tcgcgccgaa atcggtaatg
    13681 gcaacggtat cgccgctgcg tttggagatt tttttgcctt gttcgttaag aatcatcggc
    13741 aggtggccgt attcaggcag ggtagcgccg atggctttta agatgttgat ttgtttcggg
    13801 gtgttgttca catggtcgtc gccccggata acgtgggtaa cgcccatgtc gtagtcgtcc
    13861 acgacaacgc agaagttgta ggtcggcgtg ccgtcggcgc gggcgataat caggtcgtcg
    13921 agtgcttcgt tggggatgga gatttcgcct ttgaccaagt ccgtccactt ggttacgcca
    13981 tccaaaggcg ttttgaagcg gacgacgggt tgcacgccgg cagggatttc gggcagggtt
    14041 ttgcctgctt ccggccgcca gcggcggtcg taagtcgccg tgccttcttt ttcggctttc
    14101 tcgcgcatcg cttccagctc ttctttgctg caatagcagt agtaggcatc gccttttgcc
    14161 aagagttcgg cgatgacttc tttataacgg tcgaagcggc gggtttggta caccacgttg
    14221 tcggcgttgt cgtaatcgag accgacccat tccataccgt caaggatgat attgacagat
    14281 tcggcggtgg aacgcgccaa gtcggtgtct tcgatacgca ataaaaattc gcctttatga
    14341 tggcgggcaa acgcccacga aaacaaggcg gtgcgaacgc cgccgatgtg caggtagccg
    14401 gtagggctgg gggcgaaacg ggttttgacg gtcatgatgg ctccagaatc tttaaaacgg
    14461 cttattttac tgtttttacc gtgcttgggc attaaaaatg ccgtccgaac cctgcctgcg
    14521 gataagtttc agacggcatt ttctaccgct tcaatcagac agccatccca aaaagcggct
    14581 gccccatttt tccaaacggt agagggataa cgcatatccc tcttgcagca taaagatttt
    14641 tttcttattt cccgcatcaa agcgcgtggt cggcgtggca gacatataaa cgcggacacc
    14701 caaatcctcc gccatttccg ccgcccgcgc caaatggtag ggatcgctga caatcaccac
    14761 actggcaata ccgttggcac gcaaaaccgg acggatgttg ttcaggtttt cataagtgtt
    14821 gcgcgaagtg ttttcaaaca ggatgttgcg cgccggaacc ccctgtttga gcgcgtaccg
    14881 ccgcccgacc tcggcttcgg tcatatagcc ttttttggtc cggcctcccg taaacacgat
    14941 tttgcctacc ctgcggcttt ggtaaagcgc gatggcgtgg ttgatgcgtt cccgaaaaac
    15001 cggggaaggg cgtttgtccc atgcggcggc acccaacacc agcgcggcat ccgcccggac
    15061 atacggcggc aaaacctgcc cgcccgtccg ataaaccgcc caaacggatg aggcaaacac
    15121 cagcaaaagc ggaaaaacac tcaaacagaa accgcccaac aggtaatagc gcaagccgtt
    15181 gcggctgcaa aacagccgtt ttttcacaat accgcttcga tattttccaa cggcctgccg
    15241 acagccgcct taccgtttgc caaaacaatc ggacgctcca acagggcggg atgatcggcg
    15301 atggcacgca gcagtgcgtt attgtccaaa tcggggttgt ccaaacccaa ttccttgtac
    15361 aaatcatctt tcacacgcat catcccgcgc gccgactcca agcccaattt gttgaaaata
    15421 tccttcaatt cggacaagtc gggcggcgta tccaaatatt tgaccacttc ggcagcaatg
    15481 ccgcgttctt ccaataggga caaggcggca cgcgatttgc tgcaacgcgg attgtggaaa
    15541 attttgattt cagacatgac atttccttac ttctcgacaa tccccttatt atcggcttac
    15601 acagggtttt actcaatatc ccgcctacaa ccgtaccaaa cggtttacaa tacccgaatc
    15661 gacatacaaa ggacaaaacg atgaaatact tgaatcttgc cgcaatcacc cttgccgcca
    15721 catttgccgc acataccgcc tcggcagacg aactggccgg atggaaagac aacaccccgc
    15781 aaagcctgca atcactcaaa gcccccgtac gcatcgtcaa cctttgggca acctggtgcg
    15841 gcccgtgccg aaaagagatg cctgccatgt ccaaatggta caaagcgcag aaaaaaggca
    15901 gtgtcgatat ggtcggcatc gcgctcgaca catccgacaa tatcggcaac ttcctcaagc
    15961 aaacccctgt ttcctacccg atttggcgtt acaccggggc gaacagccga aactttatga
    16021 aatcctacgg aaacaatgtc ggcgtactgc cctttaccgt cgtcgaagcc ccgaaatgcg
    16081 gatacaaaca aaccattacc ggggaagtga acgaaaaaag cctgaccgaa gccgtcaaac
    16141 tcgcccattc aaaatgccgt taaacgccgg atgccgtctg aatccgcttc agacggcatt
    16201 tttcccgccc gaccttcggt atccgccaaa cttatccact atctaaaaac aggcggaatc
    16261 tttataatcg gcactgtctt acctattgtt cagacggcat atccctgcgg acgcaaccgc
    16321 ccgaaacgat atgccgccca ttccttacag gacctcctat gatccgtttc gaacaagttt
    16381 ccaaaaccta tcccggcggt tttgaagccc tgaaaaacgt cagcttccaa atcaacaaag
    16441 gcgagatgat ttttatcgca ggacactccg gttcaggcaa atccaccgtc ctcaagctga
    16501 tttcgggcat taccaaacca agcaggggca aagtccactt caacaaccaa gacctcggca
    16561 cactgaccga caaccaaatc ggctttatgc gccaacacat cggcatcgtg ttccaagacc
    16621 acaaaatcct ctacgaccgc aacgtcctgc aaaacgtcat cctgccgctt cggattatcg
    16681 gctatccgcc gcgcaaagcc gaagaacgcg cccgcatcgc catcgaaaaa gtcggcctga
    16741 aaggacgaga attggacgat cccgtaaccc tctccggcgg tgaacaacaa cgcctgtgca
    16801 tcgcccgcgc cgtcgttcac cagcccggcc tgctgattgc cgacgaaccc tccgccaacc
    16861 tcgaccgcgc ctacgcgctc gacatcatgg aattgttcaa aaccttccac gaagcgggaa
    16921 caaccgtcat cgttgccgcc catgacgaaa ccctgatggc ggactacgga caccgcatcc
    16981 tgcgcctctc gaaaggacga ctcgcatgag catcatccac tacgcctcgc tgcacatcga
    17041 atccgcccgc accgcgctca aacagcttct gcgccaaccc ttcggcacgc tgctcaccct
    17101 cctgatgctc gccgtcgcga tgaccctgcc gctgtttatg tatttgggca ttcaaagcgg
    17161 gcaaagcgtg ttgggcaaac tcaatgaatc gccgcaaatc accgtctata tggaaaccgc
    17221 cgccgcacaa agcgacagcg ataccgtccg cagcctgctg gcgcgcgaca aacggctcga
    17281 caacatccgc ttcatcggca aagaagacgg tttggaagaa ttacagtcca atctcgacca
    17341 aaatctgatt tccatgcttg acgacaaccc cctgccggat gtctttatcg ttacccccga
    17401 cccggcaacc acgcccgccc aaatgcaggc aatctaccga gacattacca aactgcctat
    17461 ggtcgaatcc gccaacatgg atacggaatg ggtacagaca ctgtaccaaa tcaacgaatt
    17521 catccgtaaa atcttatggt ttctctccct gacgctgggt atggcgttcg tccttgtcgc
    17581 gcacaacacc atccgcctac aaatcctcag ccgaaaagaa gaaatcgaaa tcaccaaact
    17641 cttgggcgcg cccgcgtcgt ttatccgccg cccattcctt tatcaagcca tgtggcagag
    17701 catcctttcc gccgccgtca gcttggggct ttgcggttgg ctgctctccg ccgtgcgccc
    17761 attggtcgat gccattttca aaccctacgg cttgaacatc ggctggcgtt tcttctaccc
    17821 gggagaaatc gtgctggtgt tcggcttcgt catcgcgttg ggcgtattcg gcgcgtggct
    17881 tgccaccacc cagcacctgc tcggcttcaa agccaaaaaa taaaaaaaaa taaaacaccg
    17941 tcaaaaatgc cgtccgaacc cgttttcaga cggcatttca atttgccagt ataatggcgc
    18001 atttttccaa caaggaacct accatgctga ccccggaaca agtaaaagcc atgattgaag
    18061 gcgtggcaaa atgcgaacat atcgaagtag aaggcgacgg acaccatttt ttcgccgtca
    18121 tcgtttcatc agaatttgaa ggcaaggcac gccttgcacg ccaccgcctg attaaagacg
    18181 ggcttaaagc ccaactggaa agcaacgaac tgcacgcact ttccatttcg gtagccgcca
    18241 caccggcaga atggaatgcc aaagcacaat aaccgccaca caaaaatgcc gtctgaaaca
    18301 gcacaacgtt tcagacggca tttttttata tcaaaccgtt tactcgccgc gtttttccaa
    18361 agcggctacg gcaggcaact ctttaccttc caagaactca aggaacgcgc cgccgccggt
    18421 ggagatgtag ccgatttggt cggtaacgcc gaatttggca atcgccgcca gcgtgtcgcc
    18481 gccgcccgca atcgagaatg ctttgctttg ggcgatggct tcggcaaggg ctttcgtacc
    18541 gcctgcgaac tggtcaaact caaacacgcc gaccggaccg ttccaaacga ccgtgcctgc
    18601 ggctttgagc agttcggcaa gctcggcggc ggatttcgga ccgatgtcca aaatcatatc
    18661 gtcttcggca acgtcggcaa tgtctttcac gaccgcttcg gcatcggcgg caaaggcttt
    18721 ggcaacgacg acatcggtcg gcagcggcac agaaccgcct ttgtccgcca tttttgccat
    18781 aatttttttg gattcttcca ctaaatcgtg ttccgccaaa gatttaccga tggctttacc
    18841 ttcagccaac aggaaggtgt tggcgatacc gccgccgacg atgagttggt caactttatc
    18901 cgccagcgat tcgaggatgg tcagcttggt ggacactttg ctgccggcaa cgatggcaac
    18961 catcgggcgc gcgggctgtt tcagggcttt gcccaaagcg tcgagttcgc ccgccatcaa
    19021 tacgccggcg caggcaacgg gcgcggcttg agcgacggct tcggtcgaag cttgggcgcg
    19081 gtgcgcggtg ccgaacgcgt cattgacgaa cacgtcgcac aaagaagcgt aggctttacc
    19141 cagttccaaa tcgtttttct tctcgccttt gttgatacgc acgttttgca gcatcacgac
    19201 atcgcccgcg ttcaaagccg gtttgttttc gcgccagtcg ttcaatactt tcacgtcttt
    19261 gcccaacaga ccgcccaaat gcgcggcaac gggcgcaacg tcgtcttcgg ggtggaactc
    19321 gccttcagtc gggcgaccca agtggatcat cacgataacg gatgcgccgt tgtccatgca
    19381 gtattggatg gacgcgagcg aggcgcggat acgggtgtcg tcgctgattt tgccgtcttt
    19441 gaacggcacg ttcatatcgg cgcggatgag gacggttttg ccctgcacgt tttgttcggt
    19501 cagttttaaa aatgccataa tcagtccttt tcaatcagtg tttgcgatac agaaacaatt
    19561 gatgccgtct gaaggcttca gacggcatcg caacccgatc agccggatac gcgctcgatt
    19621 ttcgcgccga cgctgccgag ttttttttca atattttcat aaccgcgatc caagtggtaa
    19681 atctgttcga ccacggtttc gccgcgcgcc gccaaaccgg cgataacgag gcttgcggac
    19741 gcacgcaaat ccgtcgcctt gacgactgcg ccggaaagcc gttccacacc ctgcacaaat
    19801 gccgtattgc cctcggttgt aatgttcgcc cccatccggt tcaactcggg gacgtgcata
    19861 aagcggtttt caaaaatcgt ttccaccacg cggcagcttc cctccgccac ggcattcaat
    19921 gccataaact gcgcctgcat atccgtgggg aagccggggt ggacgaccgt gcggatgtcc
    19981 accgccttcg gacgctgccg catatcgatg gcgatccaat cgtcgcccgc ctcaatcacc
    20041 gcacctgcct caaccagttt gtccaacacc acttccatcg ttttcggcgc agcattccgc
    20101 aaaaccaccc tgccgccggt tatcgccacc gcacacagga acgtccccgc ttcaatccgg
    20161 tcggggacaa cgctgtgttc gcagccgtgc agctcgtcca caccttccac aatcattgtg
    20221 gacgtaccga tgccgctgat tttcgcgccc attttgacca ggcattccgc caaatcgacc
    20281 acttcaggct cgatggcgca gttttccaaa accgtcgtac cttccgccag cgtcgccgcc
    20341 atcagcaggt tttccgtgcc gccgacggta acgacatcca ttgccacacg cgtgccttta
    20401 agcttaccct tggctttgac gtaaccgtgt tcgataacaa tctcagcacc catcgcttct
    20461 aagcctttca aatgctggtc gacagggcgc gaaccgatgg cgcagccgcc cggcaggctg
    20521 acttgcgcct cgccgaaacg cgccagcgtc gggccaagca ccaaaatcga ggcgcgcatc
    20581 gtccggacca attcgtaagg ggcgcaggtg ttgtttaccg taccaccgtt gatttcaaat
    20641 tcactgatat tgtcggtcag gacgcgcgcg cccatcccct gaagcagctt ttgtgtggtt
    20701 ttcacatctg ccagcatagg gacgtttttc aggcgcaacg tacccgatgt cagcaaaccc
    20761 gcgcacatca gcggcaatgc cgcgtttttc gcgcccgaga ccgttatttc cccgttgagc
    20821 gggccgtttg cggagatttt cagtttgtcc acgtttgttc tttcctagtg ggtacttgta
    20881 atatttcaat actcgggaca acgcataaag catcacccga tgaaggttac agaggcggaa
    20941 ttataaggga ttttcgggaa aaatacggaa gccgcaccaa agaatttgac gaaatgccgc
    21001 gctttccgaa caaggattgt cggaagacaa aaaaagccga gttttgaaaa ctcagctttt
    21061 tttgttttat ctggtgggtc gtgagcgatt cgaacgctcg accaacggat taaaagtccg
    21121 ctgctctacc gactgagcta acgacccgat aagctcgaaa ttctatacat caagcctgat
    21181 gctgtcaacc attttgtcgg cgctcatacg gcattttatt tatcggactg ttttttcgta
    21241 taaattaacg aggtcagtat cgatgcaccc aacgcgccga acacgaccga cagcgaaacg
    21301 gaaatcggga tatgcaccca atgcattacc agcattttca caccgataaa acccaacacg
    21361 aatgccaagc cgtatttcag gaagataaag cgttccgcca catccgccag caggaaatac
    21421 atcgcccgca agcccaaaat agcgaaaata ttggaagtca gcacgataaa cggatcggtg
    21481 gtaacggcaa agacggcggg gatgctgtcc acggcaaaca cgacatcgct caattcaatc
    21541 atgaccagca ccaaaaacag cggcgtggcg atttttttac cgttttcgac agtaaaaaat
    21601 ttctcgccgt gaaattccgt gccgaccgga acgactttct tgacggcatt cagcagcctg
    21661 ctgtttgcca aatccccttc ctcatcgcct tcgggcttca tcatgtgtat accagtatag
    21721 agcaggaacg cgccgaacag atacagaatc cactcgaact gctgaaccag tgccgcgccg
    21781 acgaaaatca tgacagtgcg caataccaat gcgcccaata cgccgtacag cagcacgcgg
    21841 tgctgaaact gcggtgcgac tttgaagtag ccgaatatca tcaggaacac gaaaatattg
    21901 tcgactgcca acgatttttc caaaatgtag ccggtaaaga attccaatac tttttctttt
    21961 gcgactgccg cgccgtagcc gggattgccg gcgagtttga aatacagcca gcctgcgaac
    22021 aggcaggata cggcaaccca caagccgctc catgccaagg cttctttgac gccgacttta
    22081 tggctgctgt ttttcttcag cgaaaacata tccaaggcaa tcatgaccag cactgccgca
    22141 aaaaaaacgc cgtaaaacaa cggcgacccg atgccgggat attctgtcat ggttcaatct
    22201 cctgatttga aatgtagttg tgttaccagc tgatataaaa catcgctttt gccaaaaaga
    22261 caatcagcag catatgggta aagacgacgg tgtgtatgta tttcgaccag ccgacggtca
    22321 gtgtggaacg cgccattttg acgacggcga tggcgaagtg cgccaacacg ctgaacgcca
    22381 acaggatttt cagcgtcagc atcgtaccga aggaagtggc aaacggttcg cccaatatag
    22441 aaagatagcg gtttgccgcc atcacgatgc cgctggcgaa cagcagtccg accacaaacg
    22501 gcatcaccct gacggcgcgg taagacattg ccttttccac ttcgcgccgc gcctcgcacg
    22561 acacccgtcc cgtatgcagg acggacaaaa ccagcacttc aaaaaacacg ccgccgacaa
    22621 aggcgatggc gcaatacagg tggacgatgt gcgcgacggc ataaatactc atacgatgct
    22681 ccaaccggaa aactcggata cggattgtat cactatcgtc cccgatgtcc gcataccgct
    22741 tcccgtaccg cctcggcgat tctcgcgccc gctccgcgat gttgtgcgat aaagccgtcc
    22801 acgtgcgcct gcatctgcat tccgcccccc tcggacgata aggttttttc aacggcttcc
    22861 cgccacgcgt ccgccgactc aacccgaacc gccgcccccg atgccaaggc gtgccggcag
    22921 gcttcggaaa aattgtaggt tgaaaagccg aatatcgtcg gaacgccgca ggaaagcggt
    22981 tcgataatgt tctgacagcc cgaatcgacc agactgccgc cgacaaaagc gacatcggcg
    23041 cacaggtaat acgcatacag ctcgcccata ctgtcgccta tccacacctg cgtatcaggt
    23101 tcgaccggca aaccgtcgct gcgccgctga accttaaacc cgaagcgttt tgccgtttca
    23161 aataccgtct gaaaatgctc gggatggcgc ggcacgacga ccagcagcgc atcgccgcga
    23221 tattgttgcc acgccgccag cagtttttcc gcctcgtctt caccccgata aacgcgcgtg
    23281 ctgccgcaca cggcaaccgg cctgcctccg atgcgttttt caaactgccc cgccagcgtt
    23341 ttcatctgtt ccgacggcat caggtcgtat ttggtattgc cgcacacctg cacggatgcc
    23401 gcgcccaatt tcgccaaccg cgccgcatcc gcctccgtct gcgccagaca acccgtcagc
    23461 gaagcggcgg caggacggat caggcggcgg actttcagat aaccgctcaa cgatttttcc
    23521 gacagccgcg cattcgccaa aaacagcggc acacccgcgc gccggcattc cttcatcaga
    23581 ttgggccaga tttcggtttc catcaaaatg ccgaacatcg ggcagtgttc gcgcaaaaac
    23641 tgccgtaccc acgttttttt gtcatacgga agatagcggc attgcgcatc gggaaacaga
    23701 acttgcgcgg tttcccgtcc cgtcggggtc atctgcgtca tcagcagcgg tgcatcggga
    23761 aaacgctgcc gcaactcgcg tatcaagggc tgggcggcac gcgtttctcc gaccgaaacg
    23821 gcgtgtatcc aaaccgcgcc ggtaacggga ttcggatacg gcttgccgaa acgctcgtcc
    23881 cgatgcgccc gatatgcagg ggcacttccg gagcgtttgt ccaaataacg ccgtatccat
    23941 atcggcgcaa gcagccacaa tacatcataa agccattgga acatctttct atttcctgca
    24001 aaacaaatgc cgtccgaacg gttcggacgg catttcggca acggaatcaa atatcgtagg
    24061 ttgtcgaagc ggtatctccg cccttgcccg tccagttggt atggaaaaac tcgccgcgcg
    24121 gtttgtcggt gcgttcgtaa gtgtgcgcgc cgaagtagtc gcgctgtgcc tgcaagaggt
    24181 tggcaggcag acgttcggtc gtgtagccgt ccaagaacgt aatcgccgaa gccatgcagg
    24241 gcatagggat gccgcattcg actgccttgg caaccacttt gcgccacgcc ggcaggcagt
    24301 tttccagaat atttttgaaa tacggatccg tacccaagaa caccaaatcg ggattgtttt
    24361 catacgcgtc gcggatattg cttaagaatg cgctgcgaat gatgcacccc tcgcgccaca
    24421 gcagcgcagt gttgccgtag tccaaatccc agccgtagct ttcgcccgct tcgcggatca
    24481 gcataaagcc ttgtgcgtag gaaatgattt tggatgcaag cagggcctgt ctcaacgcct
    24541 caacccattc ttgtttgccg ccttcgacgg gcgtaacggt tcgggcgaac agcttgccgg
    24601 tctgcacgcg ctgttctttg aacgacgaaa cgcagcgggc gaataccgct tcggaaatca
    24661 gcgtcaacgg aatacccaaa tccaaagcat tgatacccgt ccatttgccc gtgccttttt
    24721 gccctgccgt atcgaggatt ttctcgacca gcggttcgcc gccttcgtct ttatagccca
    24781 aaattgccgc tgtgatttca atcagataag aatccagctc ggttttgttc cacgcggtaa
    24841 acacgcggtg catttcgtcg taggacagtc ccaaaccgtc tttcataaac tggtacgctt
    24901 cgcaaatcaa ctgcatatcg ccgtattcga taccgttatg caccattttg acaaaatgtc
    24961 ccgcgccgtc cctgccgacc cagtcacaac acggttcgcc ctgcggcgtt ttggcggcaa
    25021 tcgcctgaaa aatcggtttg acggcttccc aagcgcgctt atccccgccc ggcataatgg
    25081 acgggccgtg ccgcgcgcct tcttcgccgc cggatacgcc cgccccgaca aacaaaatcc
    25141 ctttttcggc aaggtaatgt gtccgccgtg tcgtgtcggg gtaattggca ttgccgccgt
    25201 cgataatgat gtcttcctcg tccaacaggg gcagcagttg ttcgataaag tcgtcgacga
    25261 ccgaacctgc gcgaaccatc atcatgattt ttctcggttt tgccagctta tcgaccaagt
    25321 cttgcaggga atacgcgccg acaatatccg ttcccttagc cgcgccgttt aaaaattcgt
    25381 ccaccttacc gattgttcgg ttgtaggcga caaccttaaa tccgcaatcg ttcatattca
    25441 aaatcaggtt ttgccccata accgccaaac cgattacgcc aatatcgcct ttcattgcag
    25501 gaagctccgt tatagattta atttatcgac cgcaactcta cctgatttac acttgtttaa
    25561 caatccttaa ctctttaatt ttttgaaaag atgcctttac gcttttccgt gcgtttccct
    25621 gaaggcttat aaataaaata taaaatttaa ataataaaac gatgattata ttgataggag
    25681 gaattttctg tgggtaactt ttttttattt taaaaatcat tgggatttcc gttttaaaag
    25741 ggtgtcggta aggcggattc ccttttgtgc atacctgtgg attgtttttc atgaagaata
    25801 gtttttgtgg acagtttgct tgttgtgcag atggcatcct acttttcttt accgaatggc
    25861 tgccggtgcc tttaagaacg ggaatactgt ggagatttaa gccggaagtg tgtttggaac
    25921 gtgtggaaat ggtcaggtgt cggcacgaat gtcttatttc tgcatatcgg cagagtgcgc
    25981 atccgaattt gtgtataagt ggtggaaaaa atgagatttg cgggtaaatc tcacaatatt
    26041 tcagtctgat aactttggat tgcttgtgta taaataaact ttcggatggg gatacgtaac
    26101 ggaaacctgt accgcgtcat tcccacgaac ctacatcccg tcattcccat gaaagtggga
    26161 atgatgaaat tttgagtttt aggaatttat cggaaaaaac agaaaccgct ccgccgtcat
    26221 tcccgcgcag gcgggaatcc agcctcgtcg gtacggaaac ttatcgggaa agcggtttct
    26281 ttagattttg cgttctggat tcccactttt gtgggaatga cgggattaaa gtttcaaaat
    26341 ttattctaaa taactgaaac tcaacgaact agattcccgc ttttgcggga atgacgaatt
    26401 ttaggtttct gtttttggtt ttctgttttt gagggaatga tgaaatttta agtttgagga
    26461 attta
//
LOCUS       DAWFIT010000019        26390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFIT010000019 DAWFIT010000000
VERSION     DAWFIT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.019"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..226)
                     /locus_tag="WGQ85_000775"
                     /pseudo
     CDS             complement(1..226)
                     /locus_tag="WGQ85_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..685)
                     /locus_tag="WGQ85_000776"
                     /pseudo
     CDS             complement(380..685)
                     /locus_tag="WGQ85_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     repeat_region   1125..2150
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1191..1226
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(2214..2540)
                     /gene="cas2"
                     /locus_tag="WGQ85_000777"
     CDS             complement(2214..2540)
                     /gene="cas2"
                     /locus_tag="WGQ85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0750247.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2533..3447)
                     /gene="cas1"
                     /locus_tag="WGQ85_000778"
     CDS             complement(2533..3447)
                     /gene="cas1"
                     /locus_tag="WGQ85_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0750248.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(3514..6762)
                     /gene="cas9"
                     /locus_tag="WGQ85_000779"
     CDS             complement(3514..6762)
                     /gene="cas9"
                     /locus_tag="WGQ85_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0750249.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            complement(7040..10474)
                     /gene="dnaE"
                     /locus_tag="WGQ85_000780"
     CDS             complement(7040..10474)
                     /gene="dnaE"
                     /locus_tag="WGQ85_000780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0750250.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVRIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLKNGDNSGLICLSGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILSPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITSARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGTFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10758..10994
                     /locus_tag="WGQ85_000781"
     CDS             10758..10994
                     /locus_tag="WGQ85_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750251.1"
                     /translation="MADADDQRIGQFVFEDVHQRQCGFVGEQDLQPAVGQDFFERIAA
                     YGVSDGIFTFTEQAGRDVPPVLRQFWVRRRQVLQ"
     gene            10939..11253
                     /locus_tag="WGQ85_000782"
     CDS             10939..11253
                     /locus_tag="WGQ85_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0750252.1"
                     /translation="MCRRFCGNFGYGGGRCYNNGLFLKGDIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EA"
     gene            11231..11440
                     /locus_tag="WGQ85_000783"
     CDS             11231..11440
                     /locus_tag="WGQ85_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750253.1"
                     /translation="MDTILKHKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            11733..12569
                     /locus_tag="WGQ85_000784"
     CDS             11733..12569
                     /locus_tag="WGQ85_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0750254.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
     gene            complement(12621..13913)
                     /locus_tag="WGQ85_000785"
     CDS             complement(12621..13913)
                     /locus_tag="WGQ85_000785"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0750255.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDTALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13969..15879)
                     /gene="pglD"
                     /locus_tag="WGQ85_000786"
     CDS             complement(13969..15879)
                     /gene="pglD"
                     /locus_tag="WGQ85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0750256.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVICGLAVYRPDDIAALIGRCGVKKILLAIPGATQAQ
                     RRKIIGKLEAYPCEVLTIPGIKDLMDGKLSIGTLKKISVSDLLGRDPVTPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPNITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDHIRSACDRYDQQAIRTLLINALTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15927..17102)
                     /locus_tag="WGQ85_000787"
     CDS             complement(15927..17102)
                     /locus_tag="WGQ85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0750257.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            complement(17225..17338)
                     /locus_tag="WGQ85_000788"
                     /pseudo
     CDS             complement(17225..17338)
                     /locus_tag="WGQ85_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17574..18260)
                     /locus_tag="WGQ85_000789"
     CDS             complement(17574..18260)
                     /locus_tag="WGQ85_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0750258.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
     gene            complement(18247..19824)
                     /locus_tag="WGQ85_000790"
     CDS             complement(18247..19824)
                     /locus_tag="WGQ85_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0750259.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TVQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(19817..20982)
                     /locus_tag="WGQ85_000791"
                     /pseudo
     CDS             complement(19817..20982)
                     /locus_tag="WGQ85_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21002..22075)
                     /locus_tag="WGQ85_000792"
     CDS             complement(21002..22075)
                     /locus_tag="WGQ85_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0750260.1"
                     /translation="MKIILTTSMAGLGGTETATVRLGRLLKRRGHDVILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFTYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPPEFHFRKTEKECAVLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIG
                     EEMDNLKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            complement(22072..23493)
                     /locus_tag="WGQ85_000793"
     CDS             complement(22072..23493)
                     /locus_tag="WGQ85_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0750261.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLSVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(23688..24797)
                     /gene="ribD"
                     /locus_tag="WGQ85_000794"
     CDS             complement(23688..24797)
                     /gene="ribD"
                     /locus_tag="WGQ85_000794"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0750262.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGFAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            complement(24829..25293)
                     /gene="nrdR"
                     /locus_tag="WGQ85_000795"
     CDS             complement(24829..25293)
                     /gene="nrdR"
                     /locus_tag="WGQ85_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0750263.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPSVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            complement(25322..26164)
                     /locus_tag="WGQ85_000796"
     CDS             complement(25322..26164)
                     /locus_tag="WGQ85_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0750264.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         5504 a         6463 c         7731 g         6692 t
ORIGIN      
        1 cgctgtcctt actcggtgtg gtttggccgc tgatttgtcc ttcttcgtca acttctatgg
       61 cctgacgctg tttgctgcca gcggtctgaa taatggtggc gtcaatgacg gcggcggatg
      121 ctttctctac ttttaagcct ttttcggtca gttggcagtt aatcagttcc agcaattcgg
      181 acagggtatt gtcttgcgcc agccagttgc ggtagcggca taaagtatag tggattaaca
      241 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggaac cgattcactt
      301 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg
      361 gtttttgtta atccactata ctgtaatcag ggatgctcag ttcgtcaaag cggcaaaaca
      421 ggttgaagtc gatacgggta atgaggctgt gttcgagttc gggatcggag aggctgtgcc
      481 attgtccgag cagaacggct ttgaacatgg acaacagcgg gtaggcggga cggccgcggt
      541 ggtctcgaag gtaacgggtt ctttgacggt tcaggtattg ttcgatcggt tgccaatcaa
      601 tcacctggtc caacttcaat agcgggaaac ggtcgatgtg tctggcaatc atggcttggg
      661 cggtttgttg aaagaaggtg ctcatgagaa atcccctaaa tgtcttggtg ggaatttagg
      721 ggattttggg gaattttgca aaggtctcat tttgcaagat tttagtttaa tcatcaaaca
      781 gggaacagca aaaatccccg ctgcaacggg gatttttgtt cggctctcgc ctgtgtgatt
      841 aacttcacta ggagaagcta aaatgcttaa attccttatg cgtgggaaaa agttgctgct
      901 gtccgttgaa gtgaaccaca agttcatttt agcggttgct ttgctgttaa gtcaatgcag
      961 tagctaaccc gaccacaatc aaaaaaacgc aagttttccg cctactctgt gagtgggcgg
     1021 ttttttctta tttcaaatct gtgacacaat atctgtcgcc tttcttgcca taattgcact
     1081 gtgtctcaat gactttaacg cacgttcgct tatcgcaacg gctggttgta gcgcccattc
     1141 tcatttcgca gtgctacaat tttttgtatc cattcccagc cggaaattaa gttgtagctc
     1201 cctttctcat ttcgcagtgc tacaatcttt aactttgacc gtgtgcaatc cagttagttg
     1261 tagctccctt tctcatttcg cagtgctaca atctgaccac acctatccgc cagtgcttat
     1321 gcgttgtagc tccctttctc atttcgcagt gctacaataa gccggtttat ggcaattctg
     1381 ccgcgtccgt tgtagctccc tttctcattt cgcagtgcta caatatgcct ttttttgtac
     1441 tgttgtttga acgagttgta gctccctttc tcatttcgca gtgctacaat aaattcgttt
     1501 cagatagcaa acgcagtagt gttgtagctc cctttctcat ttcgcagtgc tacaatgccg
     1561 gatatgacgg tgggcaactg gtacaggttg tagctccctt tctcatttcg cagtgctaca
     1621 atatttcgct tttgtattga ttcaaggtgc ttgttgtagc tccctttctc atttcgcagt
     1681 gctacaataa attcgtttca gatagcaaac gcggtggtgt tgtagctccc tttctcattt
     1741 cgcagtgcta caatagtatt cgtcgatgat ggaaactcga gcatgttgta gctccctttc
     1801 tcatttcgca gtgctacaat tttctgttct tcgtggatat actggaattt gttgtagctc
     1861 cctttctcat ttcgcagtgc tacaatcttc cgcgcttccg ggggttaagt cgggacgttg
     1921 tagctccctt tctcatttcg cagtgctaca atcttcatct ctgcaaccat gttgtccagc
     1981 atgttgtagc tccctttctc atttcgcagt gctacaataa acgcggcttt tcggggtttt
     2041 taccttgggt tgtagctccc tttctcattt cgcagtgcta caatcttccg cgcttccggg
     2101 tgtgaggtcg ggacgttgta gctccctttc tcatttcgca gtgctacaat acttcgacgg
     2161 gaaatcctta tttcataagg gtttcccgtt ttatttgtta tgaaaaaatg ggcttaaaat
     2221 aacaataatt ggtctgaatt gacctttttt tcttgggttt tcagctcgcc caacagcagt
     2281 ttcatggcgg catattgctt ttctgttatc tccaaacagc ggattgagcc ttcttgcgga
     2341 aggtttgcac acagtctgtt gtggtgtttt tgcaacgaat cgcggccttt gacgatacgg
     2401 ctgtatacgg aaagttgcag catttggtat ccgtctttta ataaaaactg gcggaattga
     2461 ttggcggctt tgcgctttgc cgccgtgata accggtaggt cgaagaagac gataatcctc
     2521 ataaatttgg cctcactcat attggtattc tttcaatggt aggatttcag gcagtttcag
     2581 ttgtttggcg tttttatcgg ttacgccggc ttgaaatgac gaaatcattt tgtcgatggc
     2641 agccaaggtg ctgaaatgtt gccgttcgat gctggtttga tagtagagga ttttgatgag
     2701 gtgttgtttg atgccgagcg tcagttcagt tttcagacgg ccttgttcgt acaaatgtat
     2761 cacggtcagg tcggccaatg ggcgtaaggg ttcgataaag tcatcggcga gattaaatgg
     2821 attgagttca cttctatgaa acaagcctaa tgcaggtaac caaccgtata aggtcagggc
     2881 gcgtgcaact gcggcacgga gtacggcgta ggtgtagttc aatgcggcat tgacggcgtt
     2941 gttgtcatta cgggtaaatt tttcgccaaa gagggcttga aagtagaggg cggcagcttg
     3001 ggcttcacgg tttcctgtat cgcctgaacg gacttcagat gccaaagtgc gcagccgttt
     3061 ggctgcaaga tcattgcctg tttcgtctgc gacaaatgct tggttcagga ttttttggcg
     3121 gacgatatgt tgccagagtt gcttttttaa gggttcgctt atgttcagtt gcagtttgag
     3181 gattttaagt tggcggtgat attgggcata aggcagccat tgtccgcagg gcaggaactg
     3241 ctcgtcgcag gtcagcaggg ttgcaccatg ctcggctaag gctgatagga gcggggcggt
     3301 aatcagggtt tcgcgatttt cgatgatgat aacggcgatg tcctctaatg gtacggtgtg
     3361 ggactcgccg ttttgttgaa tcagcagttg ccggcgttgc aaggatagtt tgccgccgtt
     3421 ttgaataagc aggctgcgcc aagtcatgtt tttcccctta tttttatata aaaaatccct
     3481 gcttcaggcc gcctgaaaca gggatagatt aatttaacgg acaggcgggc gttttttcag
     3541 acggcatggt ctgatttctt tgcccagttc gtcaatttgg tatttttgga atgaaagggc
     3601 ggttttgacg ccgatacctt ccagtattcc atttttgcca attttatgat caagatcatg
     3661 aatgcgtata ttgatattac cggtgcctcg atggcagctg gcaaagtaac caaacattct
     3721 agcttttttt gttataacct cgactaaatc attagggtgt aatgagaatt taaagttgaa
     3781 actatcatca ataagttgcc aatcttcttc atcttttcct tgtacaacag ccctatccgg
     3841 caaaatccct ttcgctacct gccaactgta aatcggtacc agataatact tgtcgccttt
     3901 ctcaaacaca tctacgcgca ccatggttgc gttgtcggca ataccgttat ggttgcgcac
     3961 ccatacgccg gttttctgta cttgctctac gcgtacggct tttacctgtt gggtgcggtt
     4021 gcctgcttta tcgtatttgt aaaacggctc ggcaaaggct ttggcaggat cgtctttatg
     4081 tgcttccagc cgtgctttca gtgcttcgta tagcttaggt tcgcgctccc gattgaccat
     4141 tttttccaag tctttcagtt ttaactgtgt taacggtacg cgcaacacgc tgacgccttc
     4201 gtccagtcgt ttggcggatt tgacggtctc catatgccct tgcccgctca tcttccgatt
     4261 gggcgcgcgt gaaacaaaca gtggcgtaac gtattcgtgt acggcttcgg ggcgagatga
     4321 taatttttcg gcaagcaacg tgcgcagttt ttctggggta tcggcttctt cgaattcggg
     4381 tttgccgtcc ggtttgccga agacgcgaat catgacttct tgtgcgaaaa attcccaagg
     4441 ttgtgggaag tgtgtttttt gatgcagcac ttctcctgtt tctttgtcta tggttttacc
     4501 gtcaaacgcg ttcatctctt tatagcgtac aaaacgggta attttctgct gcatggcaac
     4561 ggtcgagcag gcaacgacga cggcgtccaa ggcgtgatgg cggtcgtttt ccgcacgcac
     4621 tttgcgcaat ccccaaaagc cgcgcaacag attggtaatt tgtccgttgg atgcaaagac
     4681 acgtttcttg cctttacctg tcagccgcat acggtcggca acaaattgac acaggaaacg
     4741 gttgacgtag cgcgtgtcgt tcagattgcg ttctttaaag ccgtcttcat cgaatttttg
     4801 cagcagaatc cgttgttttt tactgcgcgg gaaacggctg gtttcgacac gcgctttaaa
     4861 ttcctgccat tcgcggctgt tgtctttgcc gttgaagtat tcgtaagggg tttgattgcc
     4921 tttgttttgg ttttcgctgc ccaataccag tactttattg ttgaaactgt cgtcccatgt
     4981 gcgcgagaac ggcagggcat ggtcgatttc gacatagcct ttttcgttca gacggcctaa
     5041 gttgatttct ttgcccgaat acaggcattt gccgtgttgt tgctcgtaca gacgcagttt
     5101 cagaatatct ttggatttgg gttctccgac aaaattgggg aaatactctc ggaatttggc
     5161 ggcggctttt tccctgtctt tgcggttttc ttcttggcgt ttctcaattt ctttgcggtc
     5221 tttaaacgat ttacctactt cccttgcagt ttcaatatgg atacgagctg gggagccgta
     5281 acggcgtacc acgccgttaa tgaccttacg tgcttgagat aaggcgcgca agacgacggg
     5341 gttgcggatt tcgtcggcgg gaatcggcgg cagataaatc ttttcttccg tattcttctt
     5401 gccgtaatgg tctccgtaga tttcggcgca ggcttcatcg taacgtttgc cttgttccat
     5461 tagaggcaca attcggcgca atgctttcaa ggaaatttgg acgaacttat cgaagctgat
     5521 gtgtttcaac agcgcttcta agatttcggg ctgtatacgg tctttcagac ggcctgtaat
     5581 gtcttcatcg gttttgaaca gggagaatgc cgtgccgatt tcgtcttgta attcgggaga
     5641 aaggtttaat ggggattttt tgtctttcaa tccttctttt tccagtgcac ggctgatggc
     5701 atggtaggcc ttcatttcca tcaatgttga ggcttcggca ttgtctttac catagcgcaa
     5761 gcctttgaaa aaggcggtat cttctaaacc cagcagctta cgggcttgtg cgtaagtcag
     5821 tttggatttt ctgtatggct cgtccataag cgtggcgcgt tcggtatcgg tcaatggccg
     5881 ctcgctgcct tgctctaaaa tacgcaggtt gttcagcttg gtcagccaga tgaaacgttc
     5941 ggctgtgtag gtgtttttag cggctttcgg ctctgccggt tcgaaggtgc aatgccccaa
     6001 cattttttga acggcatcgc cggacagggc agggcgttgc gtcatcagta gggtttcaat
     6061 accttcttta aggccgcctg aaacatgcgg attgccaaat tctttttgtt tttcaaacag
     6121 caaaatcagc tccgcctgta aatctttgcg gctgaacgta tgcgaataat cgccgcgctg
     6181 attgcggata tggccgcttt ctttctcaaa tttatttaaa gccaattcgg ccggtgtgcg
     6241 gaaatcgcct gtctgtaagg catgggcatt gtcggctacg cctttgagca aagcgccaag
     6301 ctctttatcg gcagtttcgc cctcgttttt tctttgcgac aaataaccgc ggtgtttgat
     6361 taaatgcaac aagactgccg accactctaa aggcgtcagt ttgcggtcta atgcggctgc
     6421 gcgaagttgc catggtgtat tcggtaagga tttgatcaag ccgttttcgt caaaatcagc
     6481 ggcttgtaat acgccttcgc gtttcaatag gcggcgggcc cgaagcaggc gatgggcgcg
     6541 acggcgggtc aggcggcgga cactgcgcgc caaacgcctt gccatggcaa gggagtcgcc
     6601 tgttttcggt acttcggcac gctcaaatac gcgcacgccc aaatcaatca ggcagattgg
     6661 gttttcatct tcgtcaattt ctaccatcgc ccagccgacg gatgcgatgc cgatatcgag
     6721 gccgaggatg tagttgattg gattaggttt gaaggcagcc atttattact ccgtaaacaa
     6781 cgttgcaaat aatgataata tatcttattg tcttatttat atacaaatag attattgact
     6841 tatcatctca caacggctag aatcccaaac atattgtcgc actgcgaaat gagaaccgtt
     6901 gctacaataa ggccgtctga aaagatgtgc cgcaacgctc tgccccttaa agcttctgct
     6961 ttaaggggca tcgtttattt cggttaaaaa atgccgtctg aaaccgattt tgggcttcag
     7021 acggcattta tattttgggt taccagttga cgcgcaccga ccggctgccg agcaatgttt
     7081 ccagttcgcc gaacaatgcg gagctcggtg taaccgtcca tttcggcggc acttgaagcc
     7141 tgcccgacgc tttttcgttg gcatacgaca gttgcagcgg gatgcgcggc gtgtcgggca
     7201 gttggtgggc ggcaagcagt tgtaccagtt cgccgatgtc gtgatgcggg gcgagggcga
     7261 ggctgaggct gcgggcgtag cgttcgcgtg ccgtttgcag ggtcatgact tggtttgcca
     7321 tgatgcgcag tccgtcgccg ccgccgtagt cgtcgcggct gactttggat tcgataatca
     7381 gcacttggtc ggctttgagg cagtcggcgc agttttccag cgtctgaccg ccgaccatga
     7441 tttcaacctg cccgctcaaa tcttcaaggc tgacgaaggc gattttgccg cgcttgccca
     7501 tcatcgtacg cacggcggta acgaatccgg cgaggcgcac gctgtcttgc ggcttcagac
     7561 ggcctaattt ggtcggtgcg atttggcgga cttcttgggc atacgggccg aacgggtggc
     7621 cggagaggta aaagccgatg acggtttttt cttcggcgag tttttccgat tcgctccaca
     7681 tcggcgcgtc gatgagccgc accggttcga tggcgtcttc catcatgtcg aaaagtccgc
     7741 cctgattggt gttggcggct ttttggtcgg cgttgttcat ggcgaggtcg atgtttgcca
     7801 agagcatggc gcggttgggt tcgatgctgt cgaacgtgcc gccgcgtatc aaggcttcga
     7861 gggtgcggcg gttcatgtgt tctttgccga cgcgctcgca gaagtccaag aggccggtaa
     7921 acttgccgcc gctttgccgc gcggaggtga tggattcgac ggcggcttcg cccgtgcctt
     7981 taatcgcgcc gagcgcgtag cggattttca tgtccggata cggtgtgaag cggtagtcgg
     8041 attcgttgat gtcgggcggc aggaactcga taccgttggc gcggcagtcg tcgtagaaat
     8101 gcttgagctg gtctgtgttg tccaattcag acgacatggt tgctgccata aattcggcgg
     8161 ggtaatgggc tttgagccat gcggtctggt aggaaatcag ggcgtaggcg gcggcgtggg
     8221 atttgttgaa accgtagccg gcgaattttt ccatgtagtt gaagatttcg tcggattttt
     8281 cgcgtgaaat gccttgtttt gccgcgcctt cggcaaagat ttcgcggtgt ttcaccattt
     8341 cttcgggctt tttcttaccc atggcgcggc gcaacaagtc cgcgccgccg agcgagtagc
     8401 cgccgataat ctgcgccgcc tgcattactt gttcctgata caccataatg ccgtaggtcg
     8461 gtgagaggat gccttccaac agcggatgga tgtattggaa ttcctgtccc ttcatacgcg
     8521 cgacgaagtc gggaatattg tccatcgggc caggacggta gagcgacacg aaggcgatga
     8581 gttcttcaaa cttggtcgta tgcgccgttt tcagcatttt cttcatgccg gtcgactcga
     8641 attggaagac ggcggtggtg ttcgcatcgc ggaagatttg gtaggcggcc tggtcgtcga
     8701 gcggaatttt gccgacatcg acgatatcgc cggtggtgtt tttgatgttg ttctgcgcca
     8761 tttcgataat ggtcaggttg cgcagaccca aaaagtcgaa tttcaccaaa cccacgtctt
     8821 ccacgtcgcc cttgtcgtac atggatacgg gcgaggcgga ttcgtccgcc tgatatacgg
     8881 ggctgtaatc ggaaatcttg cccggcgcaa tcaacacgcc gcccgcgtgc atacccaaac
     8941 cgcgcgtcag gtcttccagc tttttcgcca gcgtaatcag ttcgtccgct tcttccgctt
     9001 cgattaattc ctgaatctgt ggctcggtct ccatggcttt ttccaaactc aggggtttgt
     9061 tggcttccaa cggaatcagc ttggacagtt tgtcgcacag cataaacggc agttccagta
     9121 cgcgccccac gtcgcgaatc actgctttgg acgacatcgt gccgaaggta acaatctggc
     9181 tgactgcttc cgcaccgtat ttttcgcgta catattcaat cacgcggccg cggtttgcct
     9241 gacagaagtc cacgtcgaag tcgggcatgg aaacgcgttc agggttcaag aaacgttcga
     9301 acagcagcgc gtatttgagc gggtcgaggt cggtaatctt cagcgaatac gccaccagcg
     9361 agcctgcgcc cgaaccacgg cccggcccta ctgggcagcc gtgcgttttc gcccagttga
     9421 taaagtcttg tacgataagg aaataaccgg ggaatttcat ctggatgatg atgttcagct
     9481 caaaatccaa acgttcctga tattccggca ttttcgccgc ccgctccgcc tcgtcgggat
     9541 aaagctgaac catgcgttcc tgcaaaccct tgtttgagag tttgatgaga tagtcatcgc
     9601 gcgataaacc gtcgggcgtg gggaaaaggg gcaggaagtt tttgcccaat gtgatgtgca
     9661 ggttgcagcg tttggcgatt tccaccgtgt tttccaaggc ttcgggcaag tcggcgaaac
     9721 gctccagcat cgtttccggc ggaatgaaaa actggcccgg cgtgaaatcg cgcggacgtt
     9781 ttttgtccgt caatacccag ccgcccgcga tacacacccg cgcctcgtgc gcgttgaaat
     9841 cgtcgcggtt cataaactgc gtcggatgcg tcgccaccac cggcaaaccc agttcctccg
     9901 ccagcttcac gctgccggaa acgcaagcct cccattcggg gcgttcgggc aggcgttgca
     9961 gctccatata gaacgcatcg gggaaccacg ccgcatactt caacgccgcc gtacgcgccg
    10021 cgtcttcatt gccgttcaac agattcacgc ccacttcgcc gtaatgcgcg ccgctcaaac
    10081 aaatcaagcc gctgttgtcg ccgtttttca gccattcggg attgagttcc gcgtgatgga
    10141 cattacggtc tttgccgaca taggccgccg tcaaaagctc gctcaagcgc agatagcccg
    10201 catcgttgcg gataatcagc atagcgcgga acggcttgtc gggcgcattc ggattgccta
    10261 tccgcacgtc cgccgcgccg acgggcttaa tccccgcgct gcggcaggct ttatagaatt
    10321 tcaccaaacc aaattcgttc atcaaatcgc tgatgcccaa agcaggcaaa ccgtattcct
    10381 gcgctttggc aatcagtttt ttaatccgca ccataccgtc ggtaatcgaa aactcggtat
    10441 gcaggcgcag cgggatgtag gtcggctcgg tcatggcaaa atcggcgtga acaataaaag
    10501 gcgtattgta gcagggttgt ctttagatgg cggtgtaggt aatgctgttt cagattcaga
    10561 cggcatgacc tgcaaatgtt tttgagcttt tacgacggca aaaaaatgcc tcctgccgta
    10621 tggcggaggc ttcccaagga gtattgatag atataaagga ctatcaaact agttataagg
    10681 aactatatgc cttattcgga cggatggcaa gcagttaaat taattttacg ttcaaacaga
    10741 tttttgattt cgttttgatg gctgatgccg atgaccagcg tatcgggcag ttcgtgtttg
    10801 aggatgtgca tcagcgtcaa tgcggattcg tcggggaaca ggatttgcag cctgctgttg
    10861 ggcaggattt ctttgaacgg attgcggcat atggggtttc agacggcatt tttactttta
    10921 cggaacaggc ggggcgggat gtgccgccgg ttttgcggca attttgggta cggcggcggc
    10981 aggtgctaca ataacggcct ctttctgaaa ggggacatca tggaagccac tgtctatctc
    11041 gaagacaatg aatacatcgc cttgtgcgac ctcttgaaat tggccggact tgccgaaagc
    11101 ggcgggcagg cgaaagcgtt tattgccgaa gggctggtgt tgcgcaacgg cgaaaccgaa
    11161 acccgtaaaa ccgccaaaat acgcggcggc gaagtcatcg agtttgacgg cgcgcgcttg
    11221 gaaatcgccg atggatacga tcctgaagca taaagccgaa gcccttttgg gcgagccgct
    11281 tttagaagaa cccgtccgcc ctgagttgtg ggaatgctgc ggcagcgact gcggcgaggc
    11341 gtgcattcag acgatttact gggcggataa ggcacgctac gacgcgcaac ggcggaagtt
    11401 gaaagaagcg ggttgggcgg aagacgaaaa tgccgtctga aaccagttcg gcttgataaa
    11461 accacgtttt cagacagtct tttatagtag attaaaattg aaaacggtca tatcgccatt
    11521 ccctcgaaag caggaatccg gaagtttgaa atagcggtta accttaaaca tttccaataa
    11581 atcaaagtct ggattcccgc ctgcgcggga atgacggcat agcggctttt cctttgcatt
    11641 tgccatacac ttgttttttg aaatcaccgc cgccacaccg cccgacaata ttgaaacacc
    11701 ctgcccacca tttaccacgc acataacgcc ttatgataga cctgcactgc cattccaccg
    11761 tttccgacgg tatgctctcc cccgccgaag tcgtgcgcct cgcgcatcaa aacggctgca
    11821 cgctgctggc gttgaccgac cacgaccaca ccggcggcat cgccgaagcg cgtgccgaag
    11881 ccgacaggct cggtttgcgc ctgattaacg gcgtggaaat ctccgtaacg tggcgcgggc
    11941 gtaccataca cgttgtcggc ttagactttg acgaacaaga cgaaaacctg caaaacctgt
    12001 tggcgcaagt gcggcaaggc cgtctgaaac gcctcgaagc catcgccgcc aagctggaaa
    12061 aacgcggcat ttcgggcgca tacgacggcg cgctcgcgct ggcggcaaac aaagaaatgg
    12121 tcagccgcac acacgtcgcc gagttcctca ttcgggtggg acacgtcaaa aacaagcagc
    12181 aggcgttcac caaatacttg ggcgacggca aatcctgcgc cgtccgccac gaatgggcga
    12241 cgctggaaga ctgcgtctcc gccgtcaacg gcgcgggcgg gttggcggtc atcgcccatc
    12301 cgatgcgtta tgatttgtcc gccaccgcca agcgcaatct gtttgaagaa tttaaaaaac
    12361 tcggcggtgc gggtatcgaa gtccacagcg gaaactgttg caaaaacgac cgcctcaact
    12421 acgcgctttt ggcagaacgc ttcggattgt tggcaagcgc gggcagcgac ttccaccgtt
    12481 taaacgactt cagcggcggc attctcggcg cgtgtcccga actgccggaa aactgcaaac
    12541 cggtgtggac gcacttttcc cgacattgaa tgcggataaa aatgccgtct gaaaactttt
    12601 cagacggcat tttttgcggt ttaaacgttg tcgtacagtt ttcggacggt tttgccgatg
    12661 gcggcaatgc ctttttccag cgtttgagcg tcctgcgcga tgctcatgcg gatgcactcg
    12721 cccgcgtgcg ggtaatcctg cgtgtcgatg ccgacgaaga aatgttcgcc cggaataatc
    12781 agcgtacctt cggctttgag catttcgtac agggtttgcg aagaaacggg caggttttca
    12841 aaccagagcc acaggaaaat cgcgccttcg ggtttgtgga ttttcatcgg gtacgcgccc
    12901 agctcgcgct tgagcagcga gacggcggtt tgcgcctgat tgcggtaaaa cggccggatg
    12961 acttggtcgg caagccgttt catctcgccg ctttccagta gcggggcggc gatggccgcg
    13021 ccgaagcgcg tgggggcaag gttcacaatc gcgttcaggc tgctgacggc tttgacgact
    13081 tcgggcgcgg cgacaatgat gccggtgcgc acgcccggca ggccgacttt ggaaaggctg
    13141 aagcagagga tgatgttttc atgccaattc agcgttacgt cgctgtaaat gatgttgggg
    13201 aacggcattc cgtaggcgtt gtcgataatc agcggaatcc cgtgttcacg cgccaaagcg
    13261 tccaaacgcg ccatttcgcc gtcggtcaac acattgccgg tcgggttggt cgggcgcgaa
    13321 cagcaaatcg cgccgatttt gcccgctttg agttcgggca ggttttccag tgcgtcaaag
    13381 tccacgcggt atttgaagaa gcctgcttcg ccttcgtgtt cgacgttttc gattttgggc
    13441 ttgacggaaa cgaagtgctg cccttcgaca tgcacgtcgg catagccgat gtattcgggt
    13501 gcgagcggca acaaaatggc tttttctgcg gatgtgccgt ctgaaagctt gaatttgccg
    13561 ccgaagaggt tgaataaata gaaaaacgcg ttttgcgaac cgttggtcag cgcgatattg
    13621 tcggcggtca aattccagcc gtattcgcgg ttgaggaagg cggtcagcgc gtcaatcagc
    13681 gcggtatcgc cttgggggtt ggagtagtta ccgatgttct cgacagcgtg ttctgccgcc
    13741 agtttggaga atatgtcggc gaacgcccga tcgatttccg gaatgcgcgc cggattgccg
    13801 ccgccgagca tgttgacggg cttgtcgctt ttgagcgcgt cgccgaggtc gtccattagt
    13861 tggaggatgc cgctgtgttg cgtgaatttt tcgccgaatg ctgaaaactg catgtcaaac
    13921 tccgtgtgtg taaggtaggt tggtttgtac gttatgccat ctgaaggttc agacggcatt
    13981 ttttctgtgt gtttctcgta cccaaagcag gtcgcagatg ccgtcgctcg gggcaaagcc
    14041 ggtcagggcg ttgatgagca gggtgcggat tgcctgctgg tcgtaacggt cgcaggccga
    14101 acggatgtgg tcgagcaggg cggagagctc gtgccacggc agcatggtct cgttggcggt
    14161 catgatgcgc ggatggccgg ttttgcggac gttgtcgccg atgagcagct cttcgtagag
    14221 tttttcgccg ggacgcagtc cggtaatgag gatttcgatg tcgccgtcgg gttgttcggg
    14281 tgttttgggt ttgaggccgc ttagggtaat catttgtcgg gcaaggtcga tgattttgac
    14341 ggattcgccc atgtcgagga cgaatacgtc gccgcccgta cccatcgcgc ccgcctgtat
    14401 gacgagttgg gcggcttcgg gtatggtcat gaaataacgt gtgatgttgg ggtgggtcag
    14461 ggtaagcggg ccgccttctg caatctgttt ttcaaacagc gggacaacgg agccggacga
    14521 acctaaaaca ttgccgaaac gtaccatgct gaagcgggtt ttttgtccgg gttcggcggc
    14581 gagcgcctga aggcagagtt ccgccatgcg tttgctggcc cccatggtgt tggtggggcg
    14641 gacggctttg tcggtggaga tgaggacgaa ggttcttacg cccgaagccg tggcggcaag
    14701 cgcgcattcg agtgtgccga aaatgttgtt gcgtatgcct tcgacggtgt tgaattcgac
    14761 catggggacg tgtttgtagg cagcggcgtg atagacggtc gcaacggaaa aggcggtcat
    14821 gacgtgttcg agcagcgtgc ggttttgcac cgaaccgaga aagggcagga tttcggtgtc
    14881 gaggcgtttt tggatgcagg tttcgcgcaa ttctttttcg atggcgtaca gggcgaattc
    14941 ggataactcg aacagcagca gcttttcggg gcggcgacgg ataatctggc ggcagagttc
    15001 cgaaccgatg gagccgcccg cgccggttac catgacggtt ttgccttcga tgtcggcatt
    15061 catcaggcgg tcgtcgggcg tgacggggtc gcgcccaagc aggtcggata cggagatttt
    15121 tttgagcgtg ccgatgctga gtttgccgtc catcaggtct ttgatgccgg ggatggtcag
    15181 cacttcgcac ggataggctt ccagtttgcc gatgattttg cggcgttgcg cctgggtcgc
    15241 gccggggatg gcgagcagga tttttttcac gccgcagcgt ccgatgaggg cggcgatgtc
    15301 gtcgggacgg taaacggcaa ggccgcagat gacggtgtgc cacagtttgg ggtcgtcgtc
    15361 cacaaaggcg gcggcggaat attcgcgcat ttgtttgacg gcctcgagaa gctgtctgcc
    15421 cgcctgcccc gcgccgtaaa tgatgacggg gatcatctgt tttttggggt gttcggacaa
    15481 cagtccgcgc aaaaccatac gcgagccggt cacggaaaca aacagcagta agaaatagac
    15541 aatcggcagg gcgaggcgca gcctttcttc aaaaatcaat gtgttgagga agaacaggac
    15601 ggcggaaaca atgctgccgg caaaagcggt ggtcaggacg tggaaactga caaagcgtgt
    15661 aacggcacgg tagagtccca ttctgacgaa caggccgatg gtcaacggcg cggtcaatag
    15721 gaaagactgc cagttggcag ggtcgaacca ttcgttcgag tagtcggcct ttaggctttg
    15781 ggtgaaccaa aaggcaatga aaatcatcag aaaatcgtgt atgaggaaac agattttctt
    15841 gatgttgcgc ggtagggcaa gcagggtttc cagagtcatg ttgtcggcgg cgtgtgtttt
    15901 tcagacggca tatgccgtct gaagggttat cgtgtggctt cggtcaatac ggcttcgatg
    15961 tgttctttgc agaacgcgat ttcgccgtcg gtcagcgttg ggtgcaccaa gaacatcagg
    16021 ctggtgtcgc ccagttcgac agcatttttc aaacgctctt tcggccgcca cggcgtattg
    16081 tcgaaggctt tttccaaata cacttcggag cagctgcctt gatagcaggg gactttgcgc
    16141 gcgttcagtt cgttcacgat gcggtcgcgc gtccagccgt ctttgaggtg ttcgggtttg
    16201 acgaaggcgt agaacttata ttgcgcgtgt ccgatgtagt cggcgacttc aaccaagcgg
    16261 atacttttga atttgcctag gctttccgcc agcttggcgg cgtgcgcttg acggcaggca
    16321 gtccattcgg gcaggcgttt gagctggatg cgcccgattg ccgcctgcat ttccatcata
    16381 cgccagtttg tgccgaaact ttcgtgcagc cagcggaaac cgggcgcgtg ttcgcggtgg
    16441 tacacggcat cgtagctttt gccgtggtct ttgtacgacc acattttttc ccacagggtt
    16501 ttgtcgttgg tcgtaaccat gccgccttcg ccgcccgtgg tcatgatttt gtcttggcag
    16561 aacgaccatg cgccgacgtg tccgatgctg ccgacggatt tgcctttgta tttcgcgccg
    16621 tgcgcttggg cgcagtcttc gatgacccac aaatcatgtt cttttgccaa gtccatgatg
    16681 ccgtccattt cggcgggcat accggcgagg tggacgacga tgatggcttt ggtattcgac
    16741 gtcagcgcgg ctttgatagt ttcggcgctg atgttttggc tgttcaaatc cacatcggca
    16801 aacacgggat ttgcgcccgc gttcacaatg caggaagcgg aagccaagaa agtgcgcgag
    16861 gtaacaatca catcgttgcc cgcgcctatg ccgattgctt tgagcgcgac atcgagtgcc
    16921 agcgtgccgt tggaaagggc gacggcgtac cgcgtgccgg caaaggcggc aaattctttt
    16981 tcaaatttgc ggcattcgct gcccgtccag tagttgactt tgttggacag caggactttg
    17041 gaaacggcat cggcttcttc ttgggtaaag cagggccacg gggagaggga ggtgttcagc
    17101 atggtgtttg gtccgtcggt tttcagacgg catttccgac cctatgccgt ctgaaggggg
    17161 gcgtgttccg aagaatcggg cgcgcgccgc aggtgttgtc aaaatcggtc tgtacggggg
    17221 tgcatagtgg attaaattta aaccagtacg gcgttgcctc gccttgccgt actatctgta
    17281 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat tttaatcgct
    17341 tatgccgtcc cggtctcggg gtttttgcgc ggcagcggct ttgccggatt gcccgcgacg
    17401 gtcatgccgt ccgggatgtc gcacacgata accgcccccg cgcccattca acttccatcg
    17461 tttccaaatg gtgtcgaaaa cgtagggcgg aaagccgttt acgccccatg aataatattt
    17521 tgtcatgaaa tagtcctttt aatattgtga gtgcatgttt gattatgtaa tggttaagta
    17581 tttgaatcaa ttataggaac taaatcttga aaggagttaa cccaaaagtc gggatgatat
    17641 tctgtgttaa aactttcagg ctggtggcgg tggatgttct gtccggaggg aagcaatcct
    17701 atggttatcc agcccaaagt gttcggcgcg ataaagtctt tggaaatgtt gtcgccgata
    17761 tagatgaagc agcctgcctt gtcggcatat ttgtcttgaa gatggcggaa gcgtttgctg
    17821 tcgggctttt ccgaagagca ggcttcagag atgaggatgt cgtcaaacag ggaagacaga
    17881 cccaaggctt ccagcttcag gcgttgggtc aggcttctgc cgtcggtaat cagcgcgcgt
    17941 gcggcgaagg gcgcggtcag ttcggacagg aaatccggcg gagcatacgg agtcagcgtc
    18001 gggcggtgca gccggtattg ccacaaaaga acctgttttt ccgattcatt aaaaccacag
    18061 tggcggcaca atttgtccag ccaatctttg ccgtcggggt cgatgttgcg ccataggttg
    18121 tctgaattcc aatcgggata cagtgcggca acggtatcga tgaccgaacg gataccggag
    18181 agcttgtatt cgtattccga atacagggta tcgtccaagt cgaagacgat gacggtggtt
    18241 tcaggattaa tcgttttcat gaaccaagac cttggcatcg tagcgcaaca taatgaggtt
    18301 gttttcccag tcgtctgaaa acgggatttc ttcgtcgccg aggtattcgc gcatcaaccg
    18361 gctcggataa ttgccgcctg cggcatacgt cagcgggaag ccaccgccga agcgggggtt
    18421 gatttccacg ccgtagaatt cgccggtttc ttcgttgtag aagaactgca ccgtgataca
    18481 gccgcgcgcg ccctgcaata ccgacatttt ttccagcagc gtttggtaga ggctgttttt
    18541 gcaggtaacg cctttgctga tttcgccgga gcgcacttcc aagcgttttc gcgggatggc
    18601 gcatttcaga cggcattgac ggtcgtaata catatcgacg gtaaactcgg aaaatttatt
    18661 ttcgatgtcg ataaactgac agaacattaa tttggtgtct gccaagactt ccggtgtcag
    18721 gtcggcaggt gtgtcaatcc gttttgcacc gatggcgcgg ctgccgtcat aaggcttggc
    18781 gaagcaaggg aaaaccaact tcttgcggtc gtaaatttcg ggtgagcgta tgccgtagcg
    18841 ggcaaacaag gtggcggttt tccgtttgtc gcggcattgt gcgatgaagt ccgaatcgga
    18901 aacgacgatg tggacacttg ccgcttcaaa gcgttcgcgc tcgtctgcca gcttttgcag
    18961 ttcggtgtct atggttggga tgattaaacc gatattttcg cgtaccgcca gattgaaaat
    19021 gctgtcgata tatgaatcgg tactaatcgg cggcacttca aatgaaccgt ccgaaacatg
    19081 gcaggcagaa gacatgcggg ggttgaggtc agtggcaaaa acgctgatgc cgtctgaaaa
    19141 acgtgcggct tcggtttgga agtcctgcac caattcgaca cggcgacctg ccgacagaat
    19201 cagtatatta tttttttcat tagtttgatt tccagtgaaa taaggcatag tggcttcccc
    19261 ttccgccgaa atcccttctt tgataaagac ttttctgact gtcaaaaaca ggattttcat
    19321 atccagccaa aaactgtaat ggtcgatata ccaaacatcg caggcgaatt tttcgtccca
    19381 cgaaagcgcg ttgcgcccgt tgacctgcgc ccagccggta atgccgggtt tcatttcgtg
    19441 gcggcggttt tggaagttgt cgtacagcgg cagatattgc atcagcagcg ggcgcgggcc
    19501 gaccaggctc atgtcgcctt ttaagacatt ccataattca ggcagttcgt ccaaactagc
    19561 ggcacgcagt tttttgccga agggtgtgag ccgctcgctg tcgggcagcg gaatgccgtc
    19621 tgaatcaagc gcgtcgcgca tggaacggaa tttgaccatt ttaaaaggtt ttccgtcctt
    19681 tccggggcgt tcttgaatga agaagacggg cgaacccaga ttcttgcgga tgaggtacgc
    19741 caaaatcagg aaaacgggtg agaggacaat cagccccgat gcggatgcga tgatgtcgaa
    19801 caggcgtttg acggctttac tcatttgcca atctttcaat caggttgacg attttccgat
    19861 aggaaatgtc gcgcctgaag cggcggacga tttcatcgga ctgaacgggg tcgtttttgc
    19921 gtttcaaaat atctttggcg gcttgaacga agctgtccac gtcgccggaa cggtagtttt
    19981 cgtgcggcag caggttcaag aggctaagga cttcggcatt ggtctggctg ttcagaatcg
    20041 gtttttgcaa agccatatag tcggagagtt tgttggtaac cgactgcatg gcgtggctat
    20101 ggatggcgtt gacggcaatg tcgcagcctt tggcaatcga catcatttca gaatagggta
    20161 aataaccgta aaacttgatg gcgcgatttt catattgttt gagtttctcc aaatcgggtc
    20221 ctccgcccat aatgtgcaat tcgacgtttt cgccgtcgtc taaaagtttc cgaacgcctt
    20281 tgcacacggt ttccacgtca tagctgtaac tgagcgtgcc caagtagaaa aggcggacgg
    20341 ttttggagcg gaatcgcggg ggggggcgat ggcggcaaaa tccgtaccga tatagacggc
    20401 ttcgccgggt acgttcggat tggcttcttt ggcgcggttg agataagtct gcgatacggc
    20461 aaccagcgcg tcggcgcagc ggtaggcacg gttggcgcgt gaagcaaagg gcagcagctt
    20521 gtgcggtact tttttcaaaa acggtacgac cgaggagaaa gactccggcc atacgtcctg
    20581 cacatcgaca atcagtttgt agcccaaacg cgctttgtgt ttgcccaaca gcaggttggt
    20641 tgccatcagc ggataggcgg aatagacgat gtcttgttcg cccggccggc agtttttcag
    20701 ccattgctca aaatttttga cgaagcggta atggctggca acacgttcta aagacacgtt
    20761 tttgccgtag ccgctttctt ccaacagcat gactttcaga cggccttgcg aggcggcctc
    20821 ggcatcttcg ggccgtctga aggatttgtc gtagtggcgg aaacggctgg taatcagcaa
    20881 cacgtcgtgc gattgcgaca atcgttctgc cagataccaa aagcggttga aatacggttc
    20941 ggacggcagc gagcaataag gggcgactat ggtgatgttc atcggcggct ttcagacggc
    21001 attacatggt atacgccgcc atggtggtac ggtagatttc gtcgtcggaa cagagtgcgg
    21061 cgacgtgtgc gtgcaacctt ttgcccattg cttcgcgcaa cagcggctgt ctgacgagcg
    21121 tgtctacggc ggcgataaag gcttcttcgt cgccgaaggg gatgcagtaa cccgtttccc
    21181 cgttgacgac catttcggaa atgcccgcca tatcgtaggt aacgacgggc gtgtcgtaaa
    21241 gtccggcttc taagatgttg ttgcccacgc ctgcgccgtg gtcgccgatg cagtggggcg
    21301 tgttcaccaa aatatccacg tctttgaagt aggcggtcaa atcgcgcacg ccgccgagga
    21361 aggtaacttt gccgtctatg cccagacgtt ccgcctgcgc tttcaaattg tccatttctt
    21421 cgcctatgcc cgccacgttc aggcgtacgg gcatatcgcg gtcgaccatt tttttgaaaa
    21481 tgtccagcat cagatgcacg gcgcggacgg tgtccaaacg ggaaagcgtg ccgagtacgg
    21541 cgcattcttt ttcggttttc cggaaatgaa attccggggg gggggggagt gcgttgtagg
    21601 cgtaggcaat ccggccggcg gggaagccgt ggcggatgag tttttcgcgc tcgtgtttgc
    21661 agttgccgat gatgtacacg cccaacttgt cgaagagttt ggcgattttg gggtaggttg
    21721 ccgcgtccaa accgtgcgag tggtagatga ttttggtttt cggcgaaacg attttggcgg
    21781 caagggcgca ggcgggaacg acgcgcgcca tttgacagtc gatgatgtcg ggttgttcgc
    21841 ggcgcagcat ccgggcgtag gtaaaggtgc ttttgaggta gccggcaagc ccgccccggt
    21901 aaaaatcaac cggctgccaa cggatgcccg atgcttgcgc ttctccgaca aaaggcccgt
    21961 ccgaagaagc aaggatgacg tcgtgcccgc gccgtttgag tagccgcccg aggcggacgg
    22021 tggcggtttc cgtgccgccc aagcctgcca tagaagtggt caggatgatt ttcataatgg
    22081 aaacccttgt tttttcaaat aatgaaacag tttgtgcaaa tctttccggt ggcgcaggat
    22141 gcagcctgct agatatgccg cccatacgcc ggcaaacagg ggatagtttg ccggcgtgcc
    22201 gaagcaggtg taggccgccg aggaggccag gcagaacaat gtgtgcagat aaagcggcag
    22261 gcgtttgagc ggctgccaca ggcggcagga gctttcggtc ttgaaggcaa aaaacagcca
    22321 gaatgaggcg gcacaggcaa ccgccgcgcc gcgcgcgccg ccggatggta cggaaagccc
    22381 cagcagcagc aggtttgccg ccagcgcgcc caaggtggcg agcgcgatcg ggcgtgtttt
    22441 tcggacgacg ttcaaaccga tgccgctgat ttctaccagc gtgcaaaaca gcggcggcag
    22501 catacacgat acgacgataa accggacggc ggcgtagttt tccggcagca ggagggaggc
    22561 gaggggcgag aaaatgccgg tcaggcagac ggcggcggca agcagggcgg aggcggattc
    22621 tgccgttgcc gagaggcggg cgggcggggt gtttgcttcg attgcgcgga aaatatacgg
    22681 tgtccagacc gttgaaaaga tgctttggaa caataatgcc gccccgccga acgaaatacc
    22741 catcgaataa acgccgagct gttctaggcc ggcatatttt ttcaggaaca aacggtcggc
    22801 ggatgccagc ccccaatagg cgatgctgct tagtgcgatc ggtatgccgt agcgcaggcc
    22861 gcgatgcagg acggcggatg aaaacggtgc gcgccggacg gccttcagac ggcatcggtt
    22921 ttgaaacagc aaaaaggcgg cggcggcaag gtttgccagc gcgtaaacgg cggtcaggac
    22981 ggcggtgttc gccggaaagt gcagcagccc gaccgtcagc ggcagcagca gcaggatggc
    23041 gagcttggac acgagttgcg cggacgaaaa ggcaagggcg cgcccttcca tacgcaaaac
    23101 cagtaagaga aagcggatgg gcaggaagct cagttcaaac agcaccagcc cgatgccggc
    23161 ggcggcatcg tcgagcgaaa acaggatttc agacggcagg gatgggcggg aaagcagcag
    23221 ggcggctatc gcggcggcag acagcagcgg cggcaggaac agggttttga acaaagtgtc
    23281 tttgtcggcg gcggcatagt attcgcggac gtatgcctga tccagcccga ggcacaatac
    23341 cgacaccgtc agtcccgccg ccgtctgcat cagcacgatg cgcccgatgt cgtcggcggg
    23401 gaaataccac gacagcagcg gcaggatgat gacggctaaa accgcgctgc cgatcgagcc
    23461 tgccgcgtag ccgaggattt cttttgtgtc catttttgat gtccgggcgg cggcgggatg
    23521 ctgcctgtgc cgtctgaagc ctttcttgat cggaatttga cggctttcag accgtcgcgg
    23581 ctgccggcgg ggtgcggcag tccgggttgc gttttttcgg gtgggcggcg gtctgaatgg
    23641 tgccgttttt tatcggcgtt attatatagt gaaacggcgc aaacccttta aaaggcgttg
    23701 ccgttttttc ggaacacggt tttgatgtcg tgtccgagga tttcgcttga aacgggtgtc
    23761 cacaagggcg gtgcggaaag ggcggcgcgg ttttcgagca gggaaaacag gtctttgccg
    23821 ctgccgagga ttttgggcga acggtacagg acgatttcgt ctgccagatt ttctgccaga
    23881 aatgcggatg tgagttcgga gcctgcttcg accatgattt cgccgaaacc ttcgtcagca
    23941 aggaggcgca tcaggtggtg caggtcgatt ttgctgtctg ccgtttcaga cggcatcagg
    24001 atgcggacgt gtgcgtgttc ccgatagggg tgcagtctgt cttcgttgcg ttcgagtgtg
    24061 gcgatgtagg ttggagattg tccgtcggtc agaaggtggc tgtccaaggg caggcgcagg
    24121 cggctgtcta acacgatgcg tgcgggttgg cgcaaagttg gaaaagcgcg gacgttgagc
    24181 cggggattgt ccgccaacac cgtgccgatg ccggtcagca ccgcgcagct ttcggcacgc
    24241 aaaacctgta cgtcggcacg cgcgtcttcg ccggtaatcc aaaagctgct gccgtctgaa
    24301 agggcggttt tgccgtccag cgaaacggcg catttgaggc ggacaaaggg gcggcggcgt
    24361 tcgatgcgcg acaggaagcc tcggttgagt tcccttgcct gatgttcgag taaaccgcat
    24421 tccgtcttga tgcctgctgc ttcgagcatg gcaaaccctt tgcctgcaac cggcgggttg
    24481 gggtcgcgca tggcggcaac gacgcgtgtt acgccggaac ggagcagagc ttcggcgcaa
    24541 ggcggtgtgc gtccgtaatg gctgcacggt tcgagggtaa caaaggcggt cgcgccttgt
    24601 gccatttcgc ccgcctgacg cagggcgtgg acttcggcat ggggttcgcc cgctttgacg
    24661 tggaagccct gcccgacaat ttggctgccg tgtgcgataa cgcagccgac gcgcggattg
    24721 ggcgaagtgg aaaaacgccc caaagcggca agtcggaggg cgttttccat catggatata
    24781 tctgtgtccg aaaacatagg gataccgtat cagtatgggt tgggggaatc aggctttgcc
    24841 gcctgttttg acggcttgcg ccagccacga ggcaaagtct gccggattgt cgaagcgttt
    24901 gtgcagggcg gcgaaacgga cggcggcttc cgtatcttgt cggagcagtt cttttagcac
    24961 catgtcggca agtgcggcag aggggatgtc gcgctgaccc gaagtgtaga gcctgtgttc
    25021 cgtcaggcgg acggtttcgt cgatctgttc gggtgtcagg gcggattttc gggcggcggc
    25081 ggtcaggtcg ttgcggaggc gttgtgcatt gaagggcgaa cgttttttgt ccggaccgat
    25141 gacggagggc attttgagtt cggcggtttc gagcgtgccg aagcgtttgc cgcagttggg
    25201 gcagtggcgg cggcggcgca cggcgttgcg ttcttccatc agacgcgaat cggcaacgcg
    25261 ggtgtcgggg tgggcgcaaa acgggcattt catggcgttt cttcctgatg ccgtctgaac
    25321 gtcaaaccga tacgccggcg gcgcgggcga tttccagacc ttcttcggca ctcatataga
    25381 cggggttttc gggacggtcg tgtcggacga tgttgccttc gcggaacatg accagttcgt
    25441 ccacggcaag ttgggaccat gattcgtcgc gggtcagtgg cagggtggcg ataacggcga
    25501 cgcggtcgga gggcgtggtt acttcggcaa aatcgaccat cacgtcgtcg tcgagcaggc
    25561 gcgccttgcc gaacggggct tggcggacga tgtagtgcag cagcgtgctg gcgtgggcaa
    25621 acagggcaat gccgtctgaa agcataaagt taaacagccc gaacttgcgg atttcgtgcg
    25681 tcagccccgc aatcgcgtca aacagcgtgt cgtcgtcggg acgggcggca aagcgggtgc
    25741 gcaggcggtt gaggatgtgg cagaacgcgc gttcggaatc ggttgtgccg acggggtgga
    25801 aaaattcgcc ctgttcgggg aaaaaatcaa tcaaatgtcc gttgtgggca aacagccagt
    25861 agccgcccca catttcgcgc ataaagggat gggtgttcgc cagcgaggtt tgtccttgcg
    25921 atgctttgcg gatatgtgcg atgacgtttt ccgatttgat ttggtaggca cgcacgaggt
    25981 cggcgacggg ggaatttgcg ctcggcttgt cgtcgtggaa caggcgcacg cctttgcctt
    26041 cgaaaaagcc gataccgaaa ccgtcggcgt gatggtcggt aatgccgccc ctgcggcgga
    26101 agccttcaaa ggaaaacata atatcggtcg gcgtattgca gttcatgccc agcagttgac
    26161 acatggtttg tcccaatgat tcagatggtc gcaagtattc ggattatacc ccgaactgaa
    26221 aatgccgtct gaaatacggc ttgttccccg ttattccctc gaaaacagaa aacagaaaac
    26281 aaagacggaa acttaagatt ccgtcattcc cgcgcaggcg ggaatccgac ttgtccggtt
    26341 ttggttgttt ttcgttccgt aacttttgag ccgtcattcc cgcgcaggcg
//
LOCUS       DAWFIT010000020        25941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFIT010000020 DAWFIT010000000
VERSION     DAWFIT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.020"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(207..494)
                     /locus_tag="WGQ85_000797"
     CDS             complement(207..494)
                     /locus_tag="WGQ85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750265.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(801..1172)
                     /locus_tag="WGQ85_000798"
     CDS             complement(801..1172)
                     /locus_tag="WGQ85_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750266.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITLEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(1306..1749)
                     /locus_tag="WGQ85_000799"
     CDS             complement(1306..1749)
                     /locus_tag="WGQ85_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750267.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1792..2661)
                     /locus_tag="WGQ85_000800"
     CDS             complement(1792..2661)
                     /locus_tag="WGQ85_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0750268.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2825..3748
                     /locus_tag="WGQ85_000801"
     CDS             2825..3748
                     /locus_tag="WGQ85_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0750269.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3826..4467)
                     /locus_tag="WGQ85_000802"
     CDS             complement(3826..4467)
                     /locus_tag="WGQ85_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0750270.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4522..5313)
                     /locus_tag="WGQ85_000803"
     CDS             complement(4522..5313)
                     /locus_tag="WGQ85_000803"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0750271.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5479..5554
                     /locus_tag="WGQ85_000804"
     tRNA            5479..5554
                     /locus_tag="WGQ85_000804"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5512..5514,aa:Thr,seq:tgt)
     gene            5589..5664
                     /locus_tag="WGQ85_000805"
     tRNA            5589..5664
                     /locus_tag="WGQ85_000805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5622..5624,aa:Thr,seq:tgt)
     gene            5806..6597
                     /gene="panB"
                     /locus_tag="WGQ85_000806"
     CDS             5806..6597
                     /gene="panB"
                     /locus_tag="WGQ85_000806"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0750272.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6587..6763
                     /locus_tag="WGQ85_000807"
     CDS             6587..6763
                     /locus_tag="WGQ85_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750273.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6720..7556
                     /locus_tag="WGQ85_000808"
     CDS             6720..7556
                     /locus_tag="WGQ85_000808"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0750274.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7721..9559
                     /locus_tag="WGQ85_000809"
     CDS             7721..9559
                     /locus_tag="WGQ85_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750275.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9699..10280
                     /locus_tag="WGQ85_000810"
     CDS             9699..10280
                     /locus_tag="WGQ85_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0750276.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            10290..11135
                     /gene="ispE"
                     /locus_tag="WGQ85_000811"
     CDS             10290..11135
                     /gene="ispE"
                     /locus_tag="WGQ85_000811"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0750277.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            11143..11218
                     /locus_tag="WGQ85_000812"
     tRNA            11143..11218
                     /locus_tag="WGQ85_000812"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11176..11178,aa:Gln,seq:ttg)
     gene            11231..11306
                     /locus_tag="WGQ85_000813"
     tRNA            11231..11306
                     /locus_tag="WGQ85_000813"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11264..11266,aa:Gln,seq:ttg)
     gene            11315..11390
                     /locus_tag="WGQ85_000814"
     tRNA            11315..11390
                     /locus_tag="WGQ85_000814"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11348..11350,aa:Gln,seq:ttg)
     gene            11557..12540
                     /locus_tag="WGQ85_000815"
     CDS             11557..12540
                     /locus_tag="WGQ85_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0750278.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12607..13179
                     /locus_tag="WGQ85_000816"
     CDS             12607..13179
                     /locus_tag="WGQ85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0750279.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            13513..15489
                     /locus_tag="WGQ85_000817"
     CDS             13513..15489
                     /locus_tag="WGQ85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0750280.1"
                     /translation="MDGRRNYGNRTEPNRTEPNRTEIIHDLENNFKQNNILKLKQLFP
                     EVFCEDQIDFEKLKLVLGAENLAGAGERYQLDWAGKTAAYLNLQSPTSRTLVPCKEES
                     ANFDGTQNVFIEAENLEALKILQKAYAGSVKMIYIDPPYNTGSDSFIYPDKFSESRDE
                     YARRVGDTDDAGYLKRDGVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTLLREDGV
                     IFISIDDNEQAQLKLLCDEVFGAENFVNQIAVKMSELSGVKMKHLNQYAKLKEFLLIY
                     AKNIHFANFNIEKKRKSPETLSKYLKYYSSIIENIESECEQWKIISLDEYFKDKNIIL
                     DREKLNDWKLSNAQRLVYRTNSKTVDKFLLKNPNAPDICKLINDDGKEIIKWGNKEML
                     FLEKYIDEYLGDIWLDISTINLNKETHTLVFENGQKPLTLIKRLLKSLETENNIVMDF
                     FSGSGTTAHAVMQLNAEDGGNRRFICVQLPEETDEKSEARKAGFDTIAEIAKERIRRA
                     GRQISDGLQDGSETDTGFKVFKLAESGFKQWRQPEQSDTEALQRELSLNIDSVLSETS
                     SENLLYELMLRMGLKLTCKVSFLDDVYFVEDEDTGGLYAFLLKRVDQGLIDAVLAKHP
                     AKVAALDRLFEGDDALKSNTVLQMKDAGVMFECV"
     gene            15724..18375
                     /locus_tag="WGQ85_000818"
     CDS             15724..18375
                     /locus_tag="WGQ85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0750281.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGLNLNTVQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNR
                     SYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRTFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGETPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKQPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFVEL
                     LEVKQAAKGRPKADLNIHYQDKKETKKKKVSVKWEDDLFEKSGGNEAYRHGFIIDGMD
                     FDKQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELAGNTDTAGVHDGYFSQDRKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWDKLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIAGRSREMDVQWEIPDILGEIQRKTGLTRRTVYEII
                     IQSGRLKDVAGNPQRFIDLTAEAVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENGFTFTVSNPQKTICENYIPLDSGTEKAFAQNCEDYDAKNEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVRDKVYFVAETKNTGKGIQTGVDMDKLRQNERLKIKCGKR
                     HFEALGGLAYRVVEKVGDLKSEKEEQA"
     gene            complement(18537..19706)
                     /locus_tag="WGQ85_000819"
     CDS             complement(18537..19706)
                     /locus_tag="WGQ85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0750282.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQVLGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            19854..21380
                     /gene="ilvA"
                     /locus_tag="WGQ85_000820"
     CDS             19854..21380
                     /gene="ilvA"
                     /locus_tag="WGQ85_000820"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0750283.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(21437..22510)
                     /locus_tag="WGQ85_000821"
     CDS             complement(21437..22510)
                     /locus_tag="WGQ85_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0750284.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(22507..23367)
                     /gene="cysW"
                     /locus_tag="WGQ85_000822"
     CDS             complement(22507..23367)
                     /gene="cysW"
                     /locus_tag="WGQ85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0750285.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(23622..24458)
                     /gene="cysT"
                     /locus_tag="WGQ85_000823"
     CDS             complement(23622..24458)
                     /gene="cysT"
                     /locus_tag="WGQ85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0750286.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            24597..24704
                     /locus_tag="WGQ85_000824"
                     /pseudo
     CDS             24597..24704
                     /locus_tag="WGQ85_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            24749..25081
                     /locus_tag="WGQ85_000825"
     CDS             24749..25081
                     /locus_tag="WGQ85_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0750287.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            25413..25922
                     /locus_tag="WGQ85_000826"
     CDS             25413..25922
                     /locus_tag="WGQ85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0750288.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         6633 a         5893 c         6970 g         6445 t
ORIGIN      
        1 aatttactcc actatacaaa acaatcaaga aactattggc ggtatgtata atcttgaatt
       61 tctttttttg atgaaattaa aataatgaaa tatatggtaa acgttacaac ggaagcctta
      121 gcctcccgat tgcagtattt caattccaag ataaatgccg catagaagat aaaatactca
      181 aatggttttt tagcggctta aatatcttat ttaatcttat tgattgtttt cccaatggtt
      241 tccgcaataa ataataagaa gtagaataat aaacaaacag aacatgaaaa taaaaataaa
      301 tctttcatta tcccagtaat aaaactccaa agattttcct tatttccatc aacaataaaa
      361 attatcagtt catgaataaa gaatactgca aagaaaatgc ttcctgccgt tagagatatt
      421 tctaatctgc ttattttccc ttcgctatat ttacctgata aacagcctaa aaaagctgca
      481 atcaaatcat ccattttaaa aatttctgta aaaacaggta atgtctttaa ttcattacct
      541 gttttgccat tatttatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga
      601 cagtacaaat agtacggcaa ggcgaggcaa cgccgtacta atccactata tatatccatt
      661 catatagaga aaaattctta tacccaatat aaaacaaggt ctttcattat attttgaaaa
      721 acctagaagc tttgtatcta ttggtttatc aaaattaata attattgcag aagacatgta
      781 ggctaataat cttgatttat ctatatttta ttaattatat atttaacccc gaagtcaata
      841 tagaaattta gtgattttaa agttctctta tcaatttcaa tctcagggag tacataaaaa
      901 tcctcagaat aggcaaactc tatatatgca tcatcattcg ataataattc aataatattt
      961 ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac atttattttt agataccatt
     1021 tttgaatatt gattaaataa ttttggatta ctatttttag taattaatcc ttccagagta
     1081 ataaaaccac tttgtataaa gtcatcactt atattaacag ccttccgaaa aaaaatataa
     1141 tcacactctt tgttatcaat ataaaatctc atactcttta ataatgaaat ataagaattt
     1201 ccagaattac aagcaatttg aaaaaacatc attactccta aaactcataa atagtaagga
     1261 atatatatat atgcatattc cttactattt tttcacaaat tatgcttatc tatgcttatt
     1321 tccataaaaa gcatgccctc gaattttttg acttgagttc tggaaatgag aataatttga
     1381 atggatttcc ttctgtccat gtctaataac attttgtctt ccccaagctt ggtttgctac
     1441 cgctcttgct tgttgagatg ttcgtgcata gacattagct cctcttgata aagcttgtgc
     1501 tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg cccgcatgca aaattcctat
     1561 tgccaccaac ggtgccgcag ccccttcagt ttctttcatc tccttttgag aaagctccgc
     1621 caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg ttttcaaatg tctgattgtc
     1681 caagccgttt gccattgcgg gggcaatcat agacagcatc attacggctg cggtgatttg
     1741 ttttttcata ataactcctt tggattacaa ggttggaaaa tcaaaacctg cttaaaacgt
     1801 atgctgtacg ccaaatttca gttcggaact gctttgccct gaaacgttga aacgtgcgga
     1861 tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg ccgaaatgag cgtaggtgga
     1921 tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg ggctgtgtgc ccagccattg
     1981 gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat gagatgttgg ggttgagcag
     2041 caggtagttg cccgatttgt agcggatgcc gtctgaaagg gttttgctgc cgttgatgcg
     2101 gtaggcggcg gtgagggaaa ggacaatcgg atctatggct ttgtaggtgg tggcgccgat
     2161 gagccaggat tttcccgacg aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag
     2221 aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa gtgtggctga tgccgaggga
     2281 tacgtcggac atccgtttgt tgcgggtttt gccgttgccg tcgagtttgc gttcttcgtg
     2341 ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg gtcagtccgt agcgcaaacc
     2401 gagcgtgccg acgagcatat cggtattgct gccgttttct tggatttcgg tcggaatggg
     2461 gataaacgag gttgcgccgg tttgaatgta aaccggtgcg gcaagttcgg cgcggttgtt
     2521 ttcgctgttc aggtaggtaa gggaagtttc cagtttccat tttcccttgt cggtcattat
     2581 gtcttcaatc gtcaagggca ggtcggcata agtggataaa ggcaggatgg cgggcaaggc
     2641 gggcaaaaag atgcgcttca tatttctcct attgatatta attcttattt actgataata
     2701 atgaatatcc gataatcttg tctaaataaa tttttaaatc ataaaaaaca atatgtttgt
     2761 ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg tgaaaaacgg ttacaatccg
     2821 ccgcatgaaa aaacgcaata atcctcttcc tctgttgaac ggtgtcaaac ccagttattt
     2881 ggtgctgccg catgaaaagc agttttacgg gctgccgctg ctgcattttc tgtgcatccg
     2941 ctttcctttt gtgggcgcgg acgattggcg caggcggttg aacagcggtt ttgtggtcgg
     3001 ttcggatggt gcgacgttgg acgaacatac tttgttcgag ccgggtaagg tgatgtttta
     3061 ttatcgggaa accagcagtg aggatgagcc gcgtattccg tttgaagaaa agattttgca
     3121 tattgatgag catttgattg tggtggacaa gccgcatttt ctgcccgtca tccccagcgg
     3181 caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg cggcctgaat tgcagcattt
     3241 gaatgttgag gacattacgc cgctgcaccg cttggacaag gatacggcag gcgtgatgct
     3301 gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg atgtttcaaa acaaaacggt
     3361 atggaaaacg tatgaggcgc ttgcgccgac aaggacggat ttgccgtatc cgctcgatgt
     3421 ggtttcgcgt ttggtgaggg gtgagaaatt ttttacgacg caagaggcgg aaggcgaacc
     3481 aaacgcacat acgacggtcg aactgattga aaacaggggg gaattcagcc tttaccgcct
     3541 tacgccgcat acgggcaaga aacaccagtt gcgcgtgcat atgatgggtt tgggtatgcc
     3601 gctgttgaat gacgcgctct atcccgtgcc gtctgaagcg ggcagcgagg attatcggaa
     3661 acctttgaag ctattggcaa aaaagattgc gtttgcagat cctttgtcgg gtcgggaaag
     3721 ggtgttttgc agcggatttt gcctataatg agagggaacg caaacccgta tcggcggatg
     3781 ccgccgatac gggttttaca ttatcgatgc cgtctgaaag gggttttatc cgttcaggca
     3841 tacgggcgcg aagcggagga tggtgtcgcg caggacgatg agtttgccgt gaaagaaatg
     3901 cgtggaaccg gcgatggtaa tgacgggcaa atcttgcggt tccgcccatt tcagtgcttt
     3961 cccgatttcg acgacttcgt cttccgcgcc gtgtatcatc agcgttttgg caacattggg
     4021 aacggcggac ggctcggggc ggccggtata gtggcagact gccgcgccga tgagcagcaa
     4081 taaatcgggt atgcgcgctt gtgcggcaaa tgtggcgaca taaccgccga aggagaagcc
     4141 ggataaggca aattcggggg cttcgggatg ttgggcacgg gcatagtcga tgacggcgag
     4201 gcagtcttgc gtttcgccgc gtccgtaatc gtgtgtgcct tcgctgccgc ctacgccgcg
     4261 aaggttgggt aggtagcagt ggaagccgag tttgcttaag gctttggcgg cagtttggat
     4321 aactttgttg gtgtttgttc cgccctggag ggggttggga tgattgatga cggcaacacc
     4381 gcgtgccggt acttgttcgg acgggatatg gatggtttct aaaatgccgg caggaccgga
     4441 tatgtgtatg gtttcgggtt tcagcataat gatttcctct tgattttccg catgttccaa
     4501 tgcagccgtg aagatagttg cttaaaggtg gaaatgcttg ccgttgttgg ggttggacgc
     4561 tttcaggccg gcaagcatac ggtggaagtc caatccgtat gcgttgctca gtttgtcggc
     4621 acgttttttg agtttgtcta tgttttgctc tttggggctg ctttggaagg ccattaccgc
     4681 gacattgccg tggctttcgg caggaagttc caagacgcgc ccttcaaaaa cgctcaacaa
     4741 ccgttcgatg aagcgttggt agcgtttgtc gccgctccac cagttggtta cgaatatgcc
     4801 gtctgaagag agtgcgtttc ggcagtctcg gaaaaacggt tcttcaacca gcgcatcgat
     4861 gatttgttcg ccgtcgaacc cgtccaccaa aatcacatcg gtgttgtggc ggaagacttt
     4921 gatatattct gcaccgtctg cttcaataat ttcaaatttc tcgccctcga aaggcaactc
     4981 aaacaggttg cgggcaatgg cgatgacctg aggattgatg tccacggcgg tttggcgcgt
     5041 gtcgggcagg taggtgtcta tccagcgtgc aaacgagccg ccgcccaagc cgatttgggt
     5101 gatatgctgc ggaagtgcgt cggtaaacag cagccagccc atcatcgcgc ggctgtaaga
     5161 gagtaccagc tcggacgggt ggtcgaggtt cattgagctt tgaacggtgt cgctgcccaa
     5221 gtgaagcgaa cggatattgc cttcttcgga aatgccgact tcgggaaagc cggattttgc
     5281 aggacgcagg cggcggtagg gatgtcttgc catcggtgtg aacggtcggt ttgaaaggcg
     5341 gatattttat cagaatgccg atgcttgtcc tgcttcaaat taatttcttt tcaaataaat
     5401 tacttattcg gatttgccgg ggctttcgga taaattcctt gccaaagtgt gaagttgcct
     5461 gcataattcg ccttctttgc cggtatagct cagttggtag agcacctgac ttgtaatcag
     5521 ggggtcccga gttcgactct tggtgccggc accatttctg ctgatggtgt tgcaaaacat
     5581 ttcagtaagc cggtatagct cagttggtag agcacctgac ttgtaatcag ggggtcccga
     5641 gttcgactct tggtgccggc accagatttg acagtccgat tgtgtaaaac agtcgggctg
     5701 ttttgcattt gcggcacggt cggtggcagg gctgcgggca tttcactata atagcccgtt
     5761 tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat atctgatgat tactgtgaac
     5821 acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta tgctgaccgc ttacgaatcc
     5881 agttttgccg cgctgatgga cgatgccggc gtggatgtgc tgctggttgg ggattctttg
     5941 ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca gcctgcgcga tatgtgttat
     6001 cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga ttgtcagcga tttgccgttt
     6061 ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg ccgccgaact gatggctgcc
     6121 ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg cggaaacgac cgaatttctg
     6181 caaatgcgcg gtattccggt ctgtgcccac atcggtctga ccccgcaatc cgtgtttgcc
     6241 ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc aggcgttgct taacgatgcc
     6301 aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg agtgtgttcc tgcggaactg
     6361 gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg gcatcggcgc gggtgtcgat
     6421 tgcgacgggc aggttttggt gatgcacgat atgctcggca ttttcccggg taagacggtg
     6481 aaattcgtca aaaactttat gcaggggcaa agcagcattc aggcggcggt tcgggcgtat
     6541 gttgccgaag tcaaagccaa aaccttccct gctgcggaac atatttttgc agactgaaca
     6601 aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg cattttgttt tgccgtgcgc
     6661 ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg aaggataagg attaccgtta
     6721 tgcaaatcat acataccatt caagaactgc gcgcgtggcg taaaaatgcg ggaacggtgg
     6781 catttgtgcc gaccatgggc aatctgcacg aagggcatct tgccctcgtt cgcgaagcca
     6841 aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa ccgcctgcaa ttcggtcagg
     6901 gcgaggattt cgacaaatat ccgcgtactt tgcaacagga tgcggacaag ctggaagccg
     6961 agggtgttgc cgttgttttc gcgcccgatg agaaagaact ctatccgaac gtggaacagc
     7021 gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg cggcaagttt cgtccgggac
     7081 atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa tattgtcgcg ccggatgttg
     7141 cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat taaaggtttg accgaggatt
     7201 tgaattttga tgttgaaata gtgcctgtcg atacggggcg cgcggaagac ggtttggcac
     7261 tctccagccg caaccagtat ttgagtgcgg cggaacgcga cgaagcaccg cgcctgtacc
     7321 gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag tttggattat gcaggtttgg
     7381 aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt ggtcgattat gtcgaaatcc
     7441 gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa gaaactggtg gtcttggccg
     7501 ccgcccgtct ggggacgacg cgcctgattg acaatttgga aataaaactc ccttaaaccg
     7561 caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca gacggcattt atttttttga
     7621 acggggtttc gcaaatctac aaacgattct gcttgtgata aagttacgcc tgattatatg
     7681 ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt atgttacccg cccgtttcac
     7741 cattttatct gtgctcgcgg cagccctgct tgccgggcag gcgtatgccg ccggcgcggc
     7801 ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga aagcagcagc gttacagcga
     7861 ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg ggcgagcggg ttaatcagat
     7921 atttacgttg ctgggagggg aaaccgcctt gcaaaagggg caggcgggaa cggctctggc
     7981 aacctatatg ctgatgttgg aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga
     8041 aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg atttatcaga aatggcggca
     8101 gattgagcct ataccgggtg aggcgcaaaa acgggcgggg tggctgcgga acgtgctgag
     8161 ggaaagagga aatcagcatc tggacggact ggaagaagtg ctggctcagg cggacgaagg
     8221 acagaaccgc agggtgtttt tattgttggc acaagccgcc gtgcaacagg acgggttggc
     8281 gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa tatgaacatc tgcccgaagc
     8341 ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa aaggaaaagg caatcggagc
     8401 tttgcagcgt ttggcgaagc tcgatacgga aatattgccc cccactttaa tgacgttgcg
     8461 tctgactgca cgcaaatatc ccgaaatact cgacggcttt ttcgagcaga cagacaccca
     8521 aaacctttcg gccgtctggc aggaaatgga aattatgaat ctggtttccc tgcacaggtt
     8581 ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc aatccgaatg cagacctgta
     8641 tattcaggca gcgatattgg cggcaaaccg aaaagaaggt gcttccgtta tcgacggcta
     8701 cgccgaaaag gcatacggca gggggacggg ggaacagcgg ggcagggcgg caatgacggc
     8761 ggcgatgata tatgccgaca gaagggatta caccaaagtc aggcagtggc tgaaaaaagt
     8821 gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg gctgcggcgg ctgtcgagtt
     8881 ggacggcggc agggcggctt tgcggcagat cggcagggtg cggaaacttc ccgaacagca
     8941 ggggcggtat tttacggcag acaatttgtc caaaatacag atgttcgccc tgtcgaagct
     9001 gcccgataaa cgggaggctt tgagggggtt ggacaagatt atcgaaaaac cgcctgccgg
     9061 cagtaataca gagttacagg cagaggcatt ggtacagcgg tcagttgttt acgatcggct
     9121 tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg ttcaggcttg cacccgataa
     9181 cgctcagatt atgaataatc tgggctacag cctgctttcc gattccaaac gtttggacga
     9241 aggcttcgcc ctgcttcaga cggcatacca aatcaacccg gacgataccg ctgtcaacga
     9301 cagcataggc tgggcgtatt acctgaaagg cgacgcggaa agcgcgctgc cgtatctgcg
     9361 gtattcgttt gaaaacgacc ccgagcccga agttgccgcc catttgggcg aagtgttgtg
     9421 ggcattgggc gaacgcgatc aggcggttga cgtatggacg caggcggcac accttacggg
     9481 agacaagaaa atatggcggg aaacgctcaa acgtcacggc atcgcattgc cccaaccttc
     9541 ccgcaaaccc cggaaataat gcaggtccat cctttcagac ggcataaggt ttgccgggaa
     9601 gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt tttgcacggc acgccgaacc
     9661 cgccggccgg caggatggca cccgttaagg aaattctgat gaaacacacc gtatccgcat
     9721 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
     9781 agccgtccga acacatcagc agttttgcag cagaagggcg gttggcagtg aaagcggaag
     9841 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
     9901 tcaatacccc tttgggcagt acgctcgggc agttgtgtca agacagggac ggcgcattgg
     9961 cagtggacgg caaaggaaat gtctatcagg cggaaagtgc ggaagaattg agcaggcagc
    10021 tggtcggttt caaactgcca atccaatatc tgcatatctg ggcagatggc aggcgtgtgg
    10081 cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga acaatacggt tggactgtcg
    10141 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
    10201 acatcaggct ggttttcacc gaaatcggta tgccgtctga aactgaaacc ccggaacgct
    10261 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
    10321 ccgcacctgc aaaactgaat ctcgatttga ggattaccgg caggcgggaa gacggttatc
    10381 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaatcga
    10441 gggatgacgg caaaatcatc ctgcacaatc cggttgatgg aatgccgcag gaagcggatt
    10501 taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa
    10561 tatggctgga caaaaaaatc ccgacagggg cgggtttggg cggcggaagc tcggatgcgg
    10621 caaccgtttt gctggtgttg aaccgttggt ggcagtgcgg tctgacgcag cggcagctca
    10681 ttgattcggg cgcggctcta ggggcggacg taccattttt tattttcgga aaaaatgctt
    10741 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtca
    10801 tcgtcaaacc gcccgtccac gtttccactg caaaaatttt cacacacgaa ggcttgacac
    10861 gaaattccgc ctcaagcata atgccgactt tccaaaacct gcaaccgttt agaaatgaca
    10921 tgcaggcagt tgtatttaaa gaataccctg aagtttggaa agcctattcc gaattgtccc
    10981 gatatggatt tgccttaatg acaggttctg gtgcgtgtgt attcacggcg tgtcaagata
    11041 ggaatagcgc atacaatata taccgacaag tttcagattt gtacgaggca tatttggcag
    11101 agggtctttc aaaacatcct ttgttgtccg tataaacatt gttggggagt cgtcaagcgg
    11161 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccaag
    11221 tcaaacaagt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
    11281 aggttcgaat ccttcctccc cagccatatt tttttgggga gtcgtcaagc ggttaagaca
    11341 ctggattttg attccagcat gcgaaggttc gaatccttcc tccccagcca gataaaagcg
    11401 tgtaagcctg cttacacgct tttatttcat agaaataaaa atattgaaat gcctttgttt
    11461 gtgtcggatg ttgcaggtat aatgtcgggc ttggtacaag cagagggaag cattgtgttt
    11521 tctgagcgga agttaaacat aaaatcaggt gagaatatgg ctgcgtacga cagtttgatg
    11581 gtatttacag gcaatgccaa tcccgaattg gcacaacgtg ttgtcaggca tttggacatt
    11641 tctttgggca atgcttccgt atccaagttt tcagacggcg aagttgccgt cgaactgttg
    11701 gaaaacgtac gcgggcgcga tgttttcatc cttcagccga cctgtgcgcc gaccaatgac
    11761 aacctgatgg aaatcctgac gatggcggat gcactgaagc gtgcttcggc aggtcgtatt
    11821 accacagcca ttccgtattt cggctatgcg cgccaagacc gccgtccgcg ttccgtccgc
    11881 gttccgattt ctgccaaact ggtggcaaat atgctgtatt cggcagggat cgaccgtgtt
    11941 ttgactgtcg atttgcatgc cgaccagatt caaggtttct tcgatattcc ggtggacaat
    12001 atttatgcca ccccgattct gttgaacgac atcaaacaac agcggattga aaatctgacc
    12061 gtcgtcagcc cggacatcgg cggtgtcgtc cgcgcccgcg ccgtggcaaa atccttgaat
    12121 gccgacttgg caatcatcga caaacgccgc ccgaaagcca atgtggcaga agtcatgaac
    12181 atcatcggcg atattcaagg cagaacctgt ctgattgtgg acgatatgat tgacactgca
    12241 aatacgctgt gcaaagccgc cgtcgctctg aaagagcggg gggcggaacg tgtcttggca
    12301 tatgccagcc acgccgtatt ctctggagag gcggtcagcc gtatcgcctc atccgaaatc
    12361 gaccaggtgg tcgtaaccga taccattcct ttgtctgaag cggctaaaaa ctgcgaccgt
    12421 atccgtcagg taacgattgc cggtctgttg gccgaaaccg tccgccgcat tagcaatgaa
    12481 gaatccgtct catatctttt caatgaagaa gtgatgacag gcagcatgtt gctgccataa
    12541 gcccgaagcc gtcttaagct ggtcgcggcc gatgacggcg attttaccta acttggagta
    12601 tttaacatga cttatgaaat tcaagcttct gttcgtgaag cacaaggcac tggtgcgagc
    12661 cgccgcctgc gtcgcgaagg ccaaatcccc ggcattctgt acggtgaagg tcaagagccc
    12721 gttgccattg ccgtggatca caaaaccgta ttctacgcat tggaaaaaga atctttccat
    12781 accgcgttga ttaaattgtc tctgaacggc gaaaccaaag acgttatcgt ccgtgatttc
    12841 caaatgcacc cgttccgccg cgaagtccaa cacatcgact tccaagccgt taaagccgat
    12901 cagcttgtac gcatccgtgt tcccctgcac atcgttaacg ctgaaaattc ccaagcggtc
    12961 aaactgcaag gcggtcgtgt atctctgttg aacacgacgg ttgaagtgct tgcgttgcct
    13021 gccaatatcc ctgctttctt ggatttggat tgtgccgaag tggttgccgg cgacattctg
    13081 cacttgtcag acatcaaact gcctgaaggt gtagaaagcg tttccctgaa acgtaacgaa
    13141 aatctggctg ttgctactgt taccggtaaa aaacgctaat tgattgaaaa gcaggcggta
    13201 cgtatatgat gcgtaccgcc ttattgtttt gggctgtgtt tcaatatttg gttgatttca
    13261 tccgtttata agggaaaacc gcttattttc gtttgggtag aaatctatct aggcgtattg
    13321 cgcgacaacg gatggttagc ttaaaaacgg catttcgtac cggatgaaaa tgaagaagat
    13381 atgtcgttca cattccggta gctgcccggc tggaaccggc tatcagagat agttggtcaa
    13441 ctttcaccct gaaaatcact tttcttttaa cttgcaacat attccgatag aatggacact
    13501 ttgtaagtaa tcattgatgg aagacgcaat tatggcaacc gaaccgaacc gaaccgaacc
    13561 gaaccgaacc gaaccgaaat tatacacgat ctagaaaata atttcaaaca aaacaacata
    13621 ttaaaactca aacaactctt tcctgaagtc ttttgcgaag accaaatcga ctttgagaag
    13681 ctcaaactcg ttctcggcgc agaaaacctt gccggtgcgg gcgaacgtta tcaactcgat
    13741 tgggcgggta aaaccgccgc ctatctcaac ctgcaaagtc ccacttcccg aaccctcgtt
    13801 ccctgcaaag aagaatctgc aaatttcgat ggcacccaaa acgtgtttat cgaagccgaa
    13861 aacctcgaag cactgaaaat cctgcaaaaa gcctacgccg gcagcgtgaa gatgatttac
    13921 atcgacccgc cctacaatac cggcagcgat tcttttattt atcccgacaa attctccgaa
    13981 agccgcgatg aatacgcccg ccgcgtgggc gataccgacg acgcgggcta cctgaagcgc
    14041 gacggcgtat tccaaggcgc gtggcgcaaa aacggcaaag acagcggcca ttaccacagc
    14101 aactggctct ccatgatgct gccgcgccta catctggcga aaaccctgct gcgcgaagac
    14161 ggcgtgattt ttatcagcat tgacgacaac gaacaggcgc agttgaaatt gttatgtgat
    14221 gaagtgtttg gggcggagaa ttttgtcaat caaattgctg tcaaaatgag tgaactttct
    14281 ggggttaaaa tgaagcattt aaatcagtat gctaaattaa aagaattttt actgatttac
    14341 gctaaaaata ttcatttcgc taatttcaat attgaaaaga aaagaaaatc tcccgaaact
    14401 ctatctaaat atttaaaata ttattcatca ataatagaaa atattgagag tgaatgtgag
    14461 caatggaaga taatatcact agatgaatat tttaaagata aaaatatcat tttagataga
    14521 gagaaattga atgactggaa gctatcaaat gcacaacgcc tagtgtatcg tacaaacagt
    14581 aaaaccgttg ataaatttct acttaaaaat ccaaatgctc ctgatatttg taaactcatc
    14641 aatgatgatg gtaaggaaat tatcaaatgg ggtaacaaag aaatgttatt cttggaaaaa
    14701 tatattgatg aatatttagg cgatatttgg ttggatatat ctactattaa tttaaataaa
    14761 gaaactcata ccttagtatt tgaaaatgga caaaaacctt taacattgat taaaagatta
    14821 ctaaaaagtt tagaaactga aaacaatatt gttatggact tcttctccgg cagcggcacc
    14881 accgcccatg ccgtgatgca actgaatgcc gaagacggcg gcaaccgccg tttcatctgt
    14941 gtccagctcc ccgaagaaac cgacgaaaaa tctgaagcgc gcaaggcggg tttcgacacg
    15001 attgccgaaa ttgccaaaga acgcatccgc cgcgcaggca ggcagatttc agacggcctt
    15061 caagacggat cggaaacaga tacgggcttt aaagttttca aacttgccga aagcggtttc
    15121 aaacaatggc ggcagcccga gcagtccgat acggaagccc tgcaacgcga attgtcgttg
    15181 aatatcgatt ctgtgctgtc cgaaacgtcg tctgaaaatc tgctctacga attgatgctg
    15241 cgtatgggct tgaagctgac ttgcaaggtt tcgtttttag acgacgtata tttcgtggaa
    15301 gacgaggata cgggcgggct gtacgctttc ctgctgaaac gcgtggatca aggtttgatt
    15361 gatgccgtgc tggcgaagca tcccgctaag gtggcggcat tggacaggct gtttgagggc
    15421 gacgatgcgc ttaaaagcaa taccgtattg cagatgaagg atgcgggcgt gatgttcgag
    15481 tgtgtgtagt cagccgaaag cgggaagtgc cgtctgccgt cattcccgcg caggcgggaa
    15541 tctagaaact caaagttgca agaatttatc ggaaatgact gaaacccaac gaacctagat
    15601 tcccactttc gtgggaatga cgatatatca gtttctgcgc cgacaggtct ggattcccgc
    15661 ctgcgcggga atgacggcaa agaggtagat tttccgcgcg acagcggata gaaggaaata
    15721 aaaatggaac tgaaatttga agaactgccc tatcagcttg atgcggtaaa tgccgttgcg
    15781 aatctttttg cagggcagcc gaaccatgcc cggacattcg atttgaccag tcaggggacg
    15841 gggcggtttg tcggcaacgg cttggatttg gattgggaaa cgctaggcct gaatctgaac
    15901 acggtgcaaa agcaaaatgg acagcctgaa acggaaatcg gcgcgcacgg gctgaatttt
    15961 tctctagaaa tggaaacggg tacgggcaaa acctatgtct atctgcgcac gatttacgaa
    16021 ttaaaccgca gttacggctg gaagaaattt gttattgtcg taccgagcgt accgatacgc
    16081 gaaggcgtgt tgaaaagcct ggaaattacg aaggcgcatt ttgacggagt gttcggcagg
    16141 cccgtgatgc gttatgcgga atattcaagc agccggctat cggacttgcg cacttttgcg
    16201 gtaaacgacc acatcgagat tttggtgatc aatatccagt cgtttgaaaa agatggaaac
    16261 gttatcaata aggtaaacga aagcggggaa acgccgatta agtttattca ggaaacgtgt
    16321 cctatcgtta ttatcgacga gccgcagaat atggaaacgg aaggccgtct gaacgctttg
    16381 gaatcgctca atccgctctt taccctgcgc tattcggcaa cgcataaaca accgtaccac
    16441 aaggtttaca gcctgaatcc cgtgcaggca tacaatttaa agctggtaaa gcaaatcgaa
    16501 gttttgccgg tattggcgga aaacgatgtg aacggcgcat ttgtggagct gctggaagta
    16561 aagcaggcgg ccaaaggccg tccgaaagcc gatttgaaca ttcattatca ggataaaaag
    16621 gaaaccaaga agaaaaaggt atccgtcaaa tgggaagacg atttgtttga aaaatcgggc
    16681 ggtaacgaag cttaccgcca cggttttatc attgacggca tggatttcga taagcaggaa
    16741 ttggcgttct caagcggtaa aaccattacg cgcggcggag acggcgatgc ggtgcgcgat
    16801 gaaattatga aaaaccaaat ccgcgaaacg gtaagggagc atttggcgaa ggagaaacgg
    16861 ctgaatccgc tggggattaa ggtgttgtct ctgttcttca tcgaccgtgt ggagaattac
    16921 cgtacagaag acggaacggc aggcaagttt gccctttggt ttgaagaaat ctatcgggaa
    16981 cttgcaggca ataccgatac ggctggcgta cacgacggct atttctcgca agacaggaaa
    17041 ggccgtctga aaaacaccga cggcacgacg caggcggaca acgataccta ccgcctcatt
    17101 atgcgcgaca aggaaaccct gctttccttt gacagcccgc tgcgctttat cttttcccat
    17161 tccgcactga aagaaggctg ggacaacccc aacgttttcc aaatctgcac gctcaacgaa
    17221 acccgttcgc cgctgaaaaa gcggcaggaa atcgggcgcg ggctgcgtat cgccgtcaat
    17281 caggaaggac ggcgcgtgcg cgatgaaaac gtgaacatac tgaccgttgt gccgaacgaa
    17341 agctacgaat tgtttgccgc caatctgcaa aaggaatacg aagacgaatg cggtattaaa
    17401 tttgccgccg aaaacatcaa aaacggcaat aagaagaaaa aacagttatt ccgcaaagat
    17461 ttcccgctcg accccgaatt tcaggcaatt tgggacaagt tgaaatacaa aacgcgctat
    17521 cgcgtgcgct tcgatacgga agaattgatc aaacaggctt cggaagcggt ggcaaacctg
    17581 ccggaaatcc aaaaaccgaa aatccgcatc cgcaaggcgc aattaaaaca atcacaaacc
    17641 tacggtatcg aaacagttga aattgccggc cgcagccgcg aaatggacgt tcaatgggag
    17701 attccggaca tactcggcga gattcaaaga aaaacggggc tgacgcggcg cacggtgtac
    17761 gaaatcatca tacagtcagg ccgtctgaaa gatgtagccg gtaatccgca acgcttcatc
    17821 gaccttaccg ccgaagcggt aaaccgcagc ctgcacgcgt tgatggcggc aggaatcgag
    17881 tatgagagaa ccggcgaagt gtacgaccaa aagctgttgg aagatatggc gcgcgaaggt
    17941 atcgagtttt acgaaaacgg ttttacattc acagtaagca atccgcaaaa aaccatttgc
    18001 gaaaactaca tccctttgga ttccggcacg gaaaaagcct ttgcccaaaa ttgcgaggat
    18061 tatgatgcga aaaatgaagg aggcgtggcg ttttacttca aactgcccgg ctggttcaaa
    18121 atcccgaccc cgctgggcaa ttacaatccc gattgggcgg tggtaaaaca ggttcgggat
    18181 aaagtgtatt tcgttgcgga aaccaaaaac accggcaaag gcattcagac cggcgtggat
    18241 atggacaagc tgcgccaaaa tgagaggctg aaaatcaaat gcggcaagcg gcattttgaa
    18301 gcgctgggcg gtttggcata ccgtgttgtg gaaaaagtgg gggatttaaa gtcggaaaaa
    18361 gaggagcagg cttaaaacaa acaggtgtaa tcgtagtatg gcgatgttgg attggggcgg
    18421 cggcaaccga agatgcggat atggtaatgg ctttgattgg gtaaaccgat cggggactga
    18481 agattgtttc agacggtctg ttgattttct tatttcggaa atgggaagat ggaaggctat
    18541 tgccctgtca gacgcgtcca aagcctttgc caccggcttc ttttttctac attttccagt
    18601 gcgacaattt ctttttcggc aatggtgtat ccgttttgtc tgattttgat ttttcctagg
    18661 acctgccctt tttttaccgg ggcgggaatc ggctgtatgg tttctagaat ttgttctgcc
    18721 attttcgctt ccttatgtgg cagagtgatg taggcttctt tgaggaagcc tgcgcggacg
    18781 gtttttttgc tgcctccgga aatttggatt tgggcaacgg ttttgccttt cggatatatt
    18841 ttgggcgtat cgaaggcctg caatgcccag ttcagcagct tgctgttgtc tgatgcgcgt
    18901 gtttccgccg attccgaacc caatgtgatg acaaggatgt gcctgccgtt gccggagtat
    18961 gacacggcaa ggttgtagcc gccgctttct gtgtgtccgg ctttcagacc gtttacattg
    19021 ttgtccctat ataaaaggat attgcggttg ttttgctcta tatttttgaa tttgaaagat
    19081 ttgatggaaa acagcgggta atattccgga aagtcgcgca tcaatgcttc agacagctgg
    19141 gcgaggtctt tggcggtgga aacctgtcct tctctactca agcctgtcgg atttttgaat
    19201 acagtgttct tcatgcccaa gcgtcgggct tctttgttca tttgttgcac aaaattttca
    19261 atcgagccgt tgcccagccg gccggcaagg gttagggcgg catcgtttgc ggatagtgca
    19321 atcatgcctt ttaagagttt gtcggtgctg accgtatcgc cgggacgtac aaacattctg
    19381 cttccttctg aagcccatgc ggattcgggt atttttaagt tttcttcaga tcggatattt
    19441 cccgatttca tgtttttgaa aaccagatat gcggtcatca gttgggttag tgccgccggt
    19501 tcgacagggg tattgatgtt tttggcggat aaaatctgtt tgctttgaag gtcgataacg
    19561 atgtgtgccg ctgtgagggt ttcgggtgtt tggaacgtgg gggcggcgtg taccgccggt
    19621 ttgttgggcg cgggcgatgc ggccgttgcg tgagaaacgc ctaagatgat ggaaagcagg
    19681 acgggcagga ttttatgtgc tgtcatgaaa tattctaatt gtgtgcgtgt ttcagtctgc
    19741 cgattatacg cttagggtgt ctgatcgggc ggatttttct tgctttcgcg ccgtcttggg
    19801 cgtatggttt tgggttttgc gattttaata aaccgattat cccatattga attatgaaca
    19861 cgccccttcc ttattccaat tacctcatcc gcatcctgac ggcatctgtc tatgatgtcg
    19921 cggtcgaaac gcctttggaa ccggcacgca gcctttctgt acgtttgaaa aacaacatcc
    19981 ttttgaaacg cgaagatttg cagccggttt tttcgttcaa aatacgcggc gcgtacaaca
    20041 aaatgtccaa gttgccgaaa gatgcgctcg cttgcggcgt gattgcggca agcgcgggca
    20101 atcacgcaca aggcgtggca ttgtccgcac agcgtttggg ttgccgtgcc gttatcgtca
    20161 tgccggagac tacgccgaaa atcaaagtgg atgcggtcaa aagccgtggc ggcgaggtgg
    20221 ttttgcgcgg tgtttcatac aacgatgctt acgattatgc gatggagctg gcggaacagg
    20281 aagggctgac ctatatcgca ccgtttgacg atcctgatgt gattgcagga caagggacgg
    20341 tagggatgga aattgtcagc cagcatcccg atccaatccg cgccgtattc gtaccgatag
    20401 gcggtggcgg tttggcggcg ggcgtggcgg catttatcaa gcaggtccgc cccgaaatca
    20461 aagttatcgg cgttcagacc aacgattcct gctgtatgaa gcagtcggtc gaagcgggtg
    20521 aaatcgtcca tttgaaagat gtcgggctgt tttcagacgg cactgcggtc aaagtcgtcg
    20581 gaaacgaaac cttccgcctc tgcaaagaac ttttggatga aatcattaca gtcgataccg
    20641 atgcggtttg cggcgcggtc aaggatattt tcgatgacac gcgcagcatt accgagccgg
    20701 cgggtgcatt ggcattggca ggtctgaaag cctatatcgc ccgagaaggc gcggaaaacc
    20761 aaaccctgat tgccgttacc agcggcgcga atatgaattt tcaccgtttg cgccacgttt
    20821 cggaacggag cgaattgggc gagggcaacg aaggtatttt tgccgttacc atccccgaag
    20881 agcgcggcag cttccttaag tttgtcaata tattgggaaa taggaatatt accgagttca
    20941 actaccgcta cggggacgat gaaaaagcgc atatctttgt cggacttcaa gcggcaggcc
    21001 cgcaggattt ggcggttatc ggcagccggt tggatgaggc gggattgccc aatgtcgacc
    21061 tgaccgatga tgagattgcc aaaatccata tccgctatat ggtcggaggg cggacggaca
    21121 aagtagaaaa cgagcgtctg gtcagttttg agtttccgga gcgtccgggg gcgttggcgc
    21181 gttttttgaa ccatatgcag ggcggttgga acattacgct cttccattac cgcaaccacg
    21241 gtgcggatta cgggcggatt ttggtcggta tcgatgtgcc gccgcacgat gccgccgcat
    21301 ttgacggttt cttggaaagt ctgggataca gctatcacga ggaaacgcaa aatgccgcgt
    21361 acaagctgtt tcttgcctga cgcttgaaag cacaatgccg tctgaaagcc tttcagacgg
    21421 cattgcgctt tcatggttaa atcgaatatt caatcagttc gttttgagag aagacataaa
    21481 cctgtttcgg aatcagcttc aattctttgc cttcggcgat tgggtaacgc gcggcatcgc
    21541 tgccagccag cgtgatatgc acgtcttgtt tgccgtgttt caccaaaata tgcgtcaatg
    21601 ccccgacggc gtggactttt tcgatttcgg cacaaatcat cggtgtttcg tgttcggcgg
    21661 cgatctgcca ttcgtgcggg cggatatagc cggtggcggt ttgttcctgc catttgtatt
    21721 gtgcgtccaa tttccacgcg aagccgttat aatgccagaa gcctttttcg atgcgtcctt
    21781 caaaagcgtc ggtttcgccg aggaactcgg taacgaaggc attttcgggt ttgcggtaaa
    21841 tagcttcggc gctgccggtt tgttcgattt tgccgtggtt catcacgacg atttcgtcgg
    21901 aaacttcgag ggcttcttct tggtcgtgcg taaccagaat gctggttaca cccaggttgt
    21961 gatggatgtc gcgcagccag gtgcgtaatt ctttgcgtac tttggcatcc aacgcgccga
    22021 agggttcgtc caaaagcagg agtttcggtt cgaccgcaag cgcacgggcg agggcgatgc
    22081 gctggcgttg cccgccggag agttggtgcg gataggattt tgccaaatgc gagagttgta
    22141 cgagtttcag caattcttcg actttggcgc ggatttgtcc tttggacggg cgttcggact
    22201 tgggcaatac ggtcaaaccg aaggcaacgt tgtcaaacac gttcatatgg cggaaaaggg
    22261 cgtagtgttg gaacacgaag ccgactttgc gctcgcgcac gtgcttggcg gttacgtctt
    22321 gcccatcaaa cagaatcttg ccgccgtcgg cgttttccag tccggcgata atgcgtaaaa
    22381 gcgtggtttt gccgcagccg gacgggccga gcagggaaac gagtttgccg gtggggacgt
    22441 tgaggttgat gtttttcagc gcgtgaaaat tgccgaagtg tttgtttaag ttttggatgg
    22501 tgatactcat actgcgttcc tttcggcggc ggcgagtttt ttgtcttgta atttggtaat
    22561 gatgttctgc accgccagcg tcgccagtgc caaaagtgcc aatacgccgg agagggcgaa
    22621 tgcgccggtg aagttgtatt cgttgtagaa gatttcgacc aaaagcggga cggtgttggt
    22681 ttcgccgcgt atgtgtcccg ataccacgct gaccgcgccg aactcgccca tcgcgcgggc
    22741 gttggtgagg atgatgccgt agagtaacgc ccatttgatg ttgggcaggg taacgcgcca
    22801 aaacatctgc cagccgcttg cgccgagtat caatgccgcc tgttcttcgc tgtcgccctg
    22861 agcctgcatc agcgggatga tttcgcgtgc gacaaagggg aaagtaacga acagcgtcgc
    22921 cagaataatg ccggggatgg cgaagataat ctgtatgcct tgcgcttcga gccagccacc
    22981 caatgccgtg tgcgcgccga acaataagac gaacattaaa ccggccacca cgggcgatac
    23041 ggaaaacggc aaatcgagca gggtagtcag caactgcttg ccgcgaaaat caaaacgggt
    23101 cagcagccac gccatcgcca cgcccaagac ggcattgacg gggacgacaa tcagcgcggt
    23161 aatcagcgtc aatttgatgg cagaccacgc ttcgggatcg tttaaggatt tcaggtacaa
    23221 atcccaaccg ccttttaagg cttcgtaaaa cacggcgacg agcggcacga ccagcatcag
    23281 cagcagaaag cccagcgcgg cggcaatcag caacacgcgc agccagcgcg gttcggtcag
    23341 gttgggattg gcggaatagg gtttcatggc ggtggtgttc cccggttggt tgttttaagg
    23401 tgttcggatg ccgtctgaaa aaccgttttt tgtggggcgg attcgttttc agacaacctt
    23461 ttattgatta agggaataaa aacgtctagc taccgtcatt cccgcgcagg cgggaatcca
    23521 ccgcagggca acaggaaaac agaaaataaa taaggcagcc gaaattcacc aatggattcc
    23581 cgcctgcgcg ggaatgacgg taacaggtat ttcagacgac ctcaaccctt cgcgcccgaa
    23641 cgcctgccca acgcccactg catcacgttc agcgcaaaca gaatcacaaa cgaaaccagc
    23701 agcataaaca acgccaccgc cgacgcgccc tgcacgtcga actgttccag cttgcccgta
    23761 ataatcagcg gcaggatttc agaaaccatc ggaatattgc ccgcgataaa aatcaccgag
    23821 ccgtattccc ccgttgcccg cgcgaacatc attcccgcgc cggtcaagag tgccggcgtg
    23881 atttccggca gcagcacgcg gcggaacgtc gtaaagcggt ttgcgcccaa agtcgccgcc
    23941 gcttcctcat attcgcccga taattcctcc aacaccggct gcacggcgcg gacgataaag
    24001 ggcaggctga cgacgaccag cgcaatccaa atgccgacgg gcgtaaacgc gattttgatg
    24061 cccaaaggct cgaaaaaacg gcctatccaa ccgttgggcg catacagggt tgccaacgcg
    24121 atacccgtaa ccgccgtcgg cagcgcaaac ggcaaatcga ccagcgcgtt cgccagaccc
    24181 ttgcccggga attcataacg caccaatacc cacgccacca gcgtgccgaa cacgacattg
    24241 gtcagcatcg cataaaacga catccgcaaa ctcagccaca ccgccgccaa cacgttcggc
    24301 tcggcaatcg tgttccaaaa gccgccccag ccgatttccg ccgccttcgc cgccatcatc
    24361 gcaaacggca agaccacaag cagcgacagg cacaatacgg tcagaccaag gctgagtttg
    24421 aagccgggca gtacgccggg cgttttgagc ggtaacataa aacaatgctg aaaataagga
    24481 aaaggaagga ctactttaac gatgccgtcc gaaaaacgga aagaatggaa agtttggtgc
    24541 aaagacgaat ttgttataaa gcggttggca gtttctcaag cgggcgcgat gttttaaaat
    24601 atagtggatt aaatttaaat caggacaagg cgacgaagcc gcagacagta caaatagtac
    24661 ggcaaggcga ggcaacgccg tactggttta aatttaatcc actataacgc cttgttttga
    24721 cggaaaacca tcatataaag gaacacttat gcagatttta tcttttcagc cggacattgc
    24781 ggaacgtatg ctggaaggta cggaaggcga gtcggtcaac gaaaacgcac aattcgtccg
    24841 tacggacaac ggctattgga ttgcgtggca tgaaggcgta gcggcactgc ttgcgcccga
    24901 tacgccgccg ggcattccct gtttttgggt ggaaggcgcg gaaagccttg aagagttgtg
    24961 cgtcatggtg gaacgcggcg agtttgacga agtggaagag tttgacggcg atgacgacga
    25021 atggctcgaa acggcacagg gttgcgggca ccacggcgac gcttgcgcct gcggacatta
    25081 aaggcattgc aggcttgccg caaggggggc aaggctttgc cgttttttaa ataatcggtg
    25141 gattgcatct gtttgcaatg ggctgaacgc ttccctttgt attttattga ataaaaaaac
    25201 ttatcttggt attataatta aggcagcatc aattattttg ggatggcaat aaacgcaaag
    25261 cattgatttg cgccgattgc agacttatta tagcaggttg cggcgcggac ttaatgggaa
    25321 ttgtttatag agcatgatgg tctgaaacat cattaattaa aaataccatt aatccgattt
    25381 atatttattt caatttcaat ggaaaaacat caatgacaat gattttaagc attttaagcc
    25441 tgttttttat catcagactg ttatttttag ccgtctctat taaacatgaa aaagccttga
    25501 ttgccaaagg ggcgaaacaa tacggaaaaa ccaattccac gctgcttgcg gcagttcata
    25561 cgctttatta tttggcgtgt tttgtttggg tatggctttc tgacactgct tttaatggca
    25621 tatccttgat tggtacgctg acggtgatgg cttcgtttgt gatattgtca ttgattatta
    25681 agcagttggg ggagatttgg acggttaaaa tctatatttt accaaatcat caaattaatc
    25741 gttcgtggtt gtttaaaaca ttccgccacc ccaattattt tttaaacatc atacccgaac
    25801 tgattggcat cgccttatta tgtcaagcgt ggtatgtttt attgattggc ctgcccattt
    25861 atttgctggt cttatttaag cgtatccgac aagaagaaca ggcgatggca acactttttt
    25921 aacccgtttc atcaattata g
//
LOCUS       DAWFIT010000021        25077 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFIT010000021 DAWFIT010000000
VERSION     DAWFIT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.021"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(970..2871)
                     /gene="thiC"
                     /locus_tag="WGQ85_000827"
     CDS             complement(970..2871)
                     /gene="thiC"
                     /locus_tag="WGQ85_000827"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0750289.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(2962..3061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3211..3287
                     /locus_tag="WGQ85_000828"
     tRNA            3211..3287
                     /locus_tag="WGQ85_000828"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3245..3247,aa:Met,seq:cat)
     gene            3469..4344
                     /locus_tag="WGQ85_000829"
     CDS             3469..4344
                     /locus_tag="WGQ85_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0750290.1"
                     /translation="MPTVHFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            4353..5291
                     /locus_tag="WGQ85_000830"
     CDS             4353..5291
                     /locus_tag="WGQ85_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750291.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(6011..7786)
                     /gene="ptsP"
                     /locus_tag="WGQ85_000831"
     CDS             complement(6011..7786)
                     /gene="ptsP"
                     /locus_tag="WGQ85_000831"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0750292.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(7786..8055)
                     /locus_tag="WGQ85_000832"
     CDS             complement(7786..8055)
                     /locus_tag="WGQ85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0750293.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(8124..8561)
                     /locus_tag="WGQ85_000833"
     CDS             complement(8124..8561)
                     /locus_tag="WGQ85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0750294.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(8642..9205)
                     /locus_tag="WGQ85_000834"
     CDS             complement(8642..9205)
                     /locus_tag="WGQ85_000834"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0750295.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(9274..10098)
                     /locus_tag="WGQ85_000835"
     CDS             complement(9274..10098)
                     /locus_tag="WGQ85_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0750296.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            10189..10821
                     /locus_tag="WGQ85_000836"
     CDS             10189..10821
                     /locus_tag="WGQ85_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0750297.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(11027..12811)
                     /gene="rmuC"
                     /locus_tag="WGQ85_000837"
     CDS             complement(11027..12811)
                     /gene="rmuC"
                     /locus_tag="WGQ85_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0750298.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(13150..13938)
                     /locus_tag="WGQ85_000838"
     CDS             complement(13150..13938)
                     /locus_tag="WGQ85_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0750299.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(13953..15302)
                     /locus_tag="WGQ85_000839"
     CDS             complement(13953..15302)
                     /locus_tag="WGQ85_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0750300.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(15321..15902)
                     /gene="petA"
                     /locus_tag="WGQ85_000840"
     CDS             complement(15321..15902)
                     /gene="petA"
                     /locus_tag="WGQ85_000840"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0750301.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(16025..16774)
                     /locus_tag="WGQ85_000841"
     CDS             complement(16025..16774)
                     /locus_tag="WGQ85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0750302.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            16905..17834
                     /locus_tag="WGQ85_000842"
     CDS             16905..17834
                     /locus_tag="WGQ85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0750303.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(18089..18481)
                     /gene="rpsI"
                     /locus_tag="WGQ85_000843"
     CDS             complement(18089..18481)
                     /gene="rpsI"
                     /locus_tag="WGQ85_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0750304.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(18491..18922)
                     /gene="rplM"
                     /locus_tag="WGQ85_000844"
     CDS             complement(18491..18922)
                     /gene="rplM"
                     /locus_tag="WGQ85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0750305.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(19100..19279)
                     /gene="ssrS"
                     /locus_tag="WGQ85_000845"
     ncRNA           complement(19100..19279)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGQ85_000845"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(19285..19590)
                     /locus_tag="WGQ85_000846"
     CDS             complement(19285..19590)
                     /locus_tag="WGQ85_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0750306.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(19601..19930)
                     /locus_tag="WGQ85_000847"
     CDS             complement(19601..19930)
                     /locus_tag="WGQ85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750307.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(19985..20974)
                     /locus_tag="WGQ85_000848"
     CDS             complement(19985..20974)
                     /locus_tag="WGQ85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0750308.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(21097..23799)
                     /gene="ppc"
                     /locus_tag="WGQ85_000849"
     CDS             complement(21097..23799)
                     /gene="ppc"
                     /locus_tag="WGQ85_000849"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0750309.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKTDGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHVDVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            23842..24735
                     /locus_tag="WGQ85_000850"
     CDS             23842..24735
                     /locus_tag="WGQ85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0750310.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGTAR"
BASE COUNT         5446 a         6759 c         6680 g         6192 t
ORIGIN      
        1 gtttcagcta tttagaataa attttgaaac tttaaccccg tcattcccac gaaagtggga
       61 attcagaacg taaaatctca agaaaccgtt ttacccgata agtttctgtg ccgacagacc
      121 tagattcccg cctgcgcggg aatgacggga tttgaggttg cggcatttat cgggagcaac
      181 agaatccgct ctgccgtcat tcccacgaaa gtgggaatct aggacgcaaa atctcaagaa
      241 atcgttttgc ccgataagtt tccgcaccga cagacctaga ttcccgcctg cgcgggaatg
      301 acgaatccat ccatacggaa acctgcacca cgtcattcct acgaacctac attccgtcat
      361 tcctacgaaa gtgggaattc agaacgtaaa atctcaagaa accgttttac ccgataagtt
      421 tctgtgccga cagacctaga ttcccgcctg cgcgggaatg acgggatttg agattgcggc
      481 atttatcggg agcaacagaa tccgctctgc cgtcattccc acgaaagtgg gaatccagga
      541 cgcaaaatct caagaaaccg ttttgcccga taagtttccg caccgacaga cctagattcc
      601 cgcctgcgtg ggaatgacgg cggaaagatt ctatttttcc cgataatcac ccacaatctc
      661 aaattccttc attctctcaa aaacaaaatc agaaccctaa atcccatcat ccccatctat
      721 gtgaatataa aaattttaaa aattatagtg gattaacaaa aaccagtacg gcgttgcctc
      781 gccttagctc aaagagaacg attctctaag gtgctcaagc accaagtgaa tcggttccgt
      841 actatctgta ctgtctgtgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt
      901 ttcacaagcg aaagaatgcc gtctgaagcc ttttttccgg gtttcagacg gcattttttg
      961 cttgacgttt caactgtaaa tcttcgcgcc ttttttgacg aactcgaccg ctttttcctc
     1021 catgccctgc cgttgggctt tttgcttgtc ggcgtagtcg cgcacttcct gcgtgatttt
     1081 catcgagcag aatttggggc cgcacattga gcagaagtgg gcgattttcg cgccttcggc
     1141 aggcagggtt tcgtcgtgga agctttcggc acgttcaggg tcgaggctta agcgaaattg
     1201 gtcgcgccaa cggaactcga aacgcgcctt gctcagggcg ttgtcacgta attgtgcgcc
     1261 cggccagcct ttggcgagat cggcggcgtg ggcggcgagt ttgtaggtga tgatgccggt
     1321 gcgcacgtct tctttgtcgg gcagccccaa atgttctttg ggggtaacgt aacaaagcat
     1381 cgccgtgccg taccagccga tattggccgc gcctatgccc gaggtgatgt ggtcgtagcc
     1441 gggtgcgatg tcggtaacga gcgggccgag tgtgtaaaaa ggtgcttcaa agcagtgttg
     1501 cagctcttcg gtcatgtttt ctttgacgcg ttgcagcggc acatggccgg ggccttcgat
     1561 catgacttgt acgtcatgtt tccacgcctt atcggtcaat tcgcccaagg tgtgcagctc
     1621 ggcgaattgg gattcgtcgt tggcatcggc aatgcagccg gggcgcaggc cgtcgccgag
     1681 gctgaacgac acatcatacg ctttcatgat ttcgcagatt tcgtcgaaat gcgtgtagag
     1741 gaagttttcc cgatgatgtg ccaaacacca tttcgccata atcgaaccgc cgcgcgagac
     1801 gatgccggtg aggcggttgg cggtcatcgg tacataacgc agcaacacgc ccgcgtgtat
     1861 ggtgaaatag tccacgcctt gttccgcctg ctcgattaag gtgtcgcgga acaaatccca
     1921 agtcaaatct tcggcaatgc cgccggtttt ttccaaagcc tgataaatag gcacggtgcc
     1981 gatggggacg ggcgcgttgc ggataatcca ttcgcgcgtt tcatggatgt gcgcgcctgt
     2041 ggataagtcc ataattgtgt ccgcgcccca acgcagcgac cacaccattt tttcgacttc
     2101 ttcggtcagg ctggaagtga cggcggaatt gcccaagttg ccgttgattt tgacgcggaa
     2161 gttgcggccg ataatcatcg gttcgagttc gggatggttg atgttggcgg ggataatcgc
     2221 gcgtccggcg gcgatttctt ggcgcacgaa ttcgggcgtg atttggtcgg gacgggttgg
     2281 gatgttcgcg ccgaaacttt gccctgcgtg ttgtttcaag agtttggcgt attccgattt
     2341 ttgggacaat tcgtctaatt ttaaacgttc gcgtatggcg acaaactcca tttcgggcgt
     2401 gataatgcct tggcgcgcgt agtgaagctg ggttacgttg ctaccgcttt tcgcgcggcg
     2461 cgggcgggtg atttggttga agcgcagatg ggcggttttc ggatcgtgtg cgcgttcgat
     2521 gccgtattcg ctggagagct tgggcaggat ttcggtatcg ccgcgttcgt tcagccatgc
     2581 ggtgcggatg tgcggcagac cttgtttcag gtcgatgtgt gccgctggat cgccgtacac
     2641 gccgctggtg tcatagacgg gaatcggcgg gttggcttcc gcgccttgcg ccgtataggt
     2701 gtcgtcctga cggatttcac gcaaaggcac gcggatgtcg tcgcggctgc cttgcagata
     2761 cacgcgttcc gagttcggat atttgaagcg gataccgatg tcttcgctca agtcggcaag
     2821 ttcgcgcgct tcgttgccgg aagttttggc ggtttttttt ggcgtagtca taaaaaatgc
     2881 tcctgttttc tcgtttagaa taaagaaaca ggagcgtttt gcgttttcag acggcatttg
     2941 aaaaccaatg ctgtctgaaa gacagaatcc gtgaaaactc cccacgcagg tattatcccg
     3001 atcgggtgta aagggtattt ctcagccgcc tgaacatcat gcagcacccc tgtttcaatg
     3061 ttaaccaaaa ttaaatcacg aacatgaact tttgtaaaga aaatgatatt tcaaatcagg
     3121 cataaaccgc cggacggcaa aattttatga tttttcgcgg aaataatgtt tgacaacata
     3181 aaaaatctgc cgtatagttt cgttttctga cgcgggatgg agcagcatgg tagctcgtcg
     3241 ggctcataac ccgaaggtcg taggttcgaa tcctgctccc gcaaccaaat atcaaacccc
     3301 tcggttcaat gccgaggggt tttgttttgc ctgtttcctg ccgcctccgt tttttgccgg
     3361 attttccttc ctgccgcaat atcggaacag cagcccgccg tctgtttgcg gttgcaaatt
     3421 caggcggttt gattacaatc ttccgcatta tgttcaagaa agccgactat gccgaccgtc
     3481 cattttaccg aatccgtcag caaacaagac cttgatgccc tgttcgagtg ggcaaaagca
     3541 agttacggtg caaaaagttg ctggaaaacg ctgtatctga accgtctgcc tttgggcaat
     3601 ctgtcgccgg aatgggcgga acgcgtcaaa aaagactggg aggcaggctg ctcggagtct
     3661 tcagacggca ttttcctgaa tgcggacggc tggtcagata tgggcggccg cttgcagcac
     3721 ctcgcccgca catggaacaa ggcggggctg cttcacggat ggcgcaacga gtgtttcgac
     3781 ctgaccgacg gcggcggcaa tatcttgttc tcactcgaac gcgccgcttt ccgtccgttc
     3841 ggactgctca gccgcgccgt ccatctcaac ggtctgactg aatcggatgg cagatggcat
     3901 ttttggatag gcaggcgcag tccgcacaaa gcagtcgatc ccgacaaact cgacaatatt
     3961 gccgccggcg gtgtttccag cggtgaattg ccgtctgaag ccgtgtgtcg cgaaagcagc
     4021 gaagaagcgg gtttggatca aacgctgttt ccgctcatcc gcccggtatc gcagctgcac
     4081 agcctgcgcc ccgtcagccg gggtgtgcac aatgaaatcc tgtatgtgtt cgatgccgtc
     4141 ctgcccgaaa ccttcctgcc tgaaaatcag gatggcgagg ttgcgtgttt tgagaaaatg
     4201 gacatcggca gcctggttgc cgccatgttg tcggaaaaca tgatgcacga cgcgcaactg
     4261 gttacgctgg acgcgttttg ccgttacggt ctgattgatg tcgcccatcc gctgtccgag
     4321 tggctggacg gcatacgttt ataggatgcg ccatgcttga actgaacgga ctctgcaaac
     4381 gcttcggcgg caaaacggtt gccgacgata tctgcctgac tgtcgggcgc ggcaaaatac
     4441 tcgccgtact ggggcggtcg ggctgcggaa aatccaccct gctgaatatg attgcgggca
     4501 tcgtccggcc ggacggcggc gaaattcagc tgaacgggga aaacattacc cgtatgccgc
     4561 ccgaaaaacg ccgtatttcg ctgatgtttc aagattacgc gctgtttccc catatgagtg
     4621 cgctggaaaa tgcggcattc ggtttgaaaa tgcaaaaaat gccgaaagcc gaagccgaac
     4681 gccttgccat ggcggcactt gccgaagtcg gactggaaaa cgaggtgcac cgcaagcctg
     4741 aaaaactttc cggaggcgag aaacaacggt tggcactggc gcgcgctttg gttgtccgcc
     4801 cttccctgct gctgttggac gaatcgtttt ccagtttgga cacgcatttg cgcggcacgc
     4861 tgcgccgcat gactgccgaa cgcatccgca agggcggcat ccctgccgtt ttggtaacgc
     4921 attcgcccga agaggcctgc acggcggcgg acgaaatcgc cgtcatgcac gaggggaaaa
     4981 tccttcaatg cggtacgccc gaaaccttgg ttcaaacgcc tgccggcgtg caggtcgccc
     5041 gactgatggg tttgcccaat accgacgatg accgccatat tccgcaacat gcggtgcgtt
     5101 tcgaccaaga cggcatggag tgccgcgtat tatcccgtac ctgtttgccc gaatcgttca
     5161 gcctgtccgt cctccatccg gaacacggca tcctgtggct gaacctcgat atgccgcacg
     5221 ccggtgaaat atcgggaaac gatacggtac gcatccatat cgaagacagg gaaatcgtcc
     5281 gcttccgctg atgcttctta aaaacaaaat gccgtctgaa aacctttcag acggcatttt
     5341 ttaccaaagc agccatattt tttatcaggg ctgcaaaatt tatccgaaac aacagaaacc
     5401 accctgccgt cattcccgca aaagcgggaa tctagaaccc aacacggcaa aaatttatcc
     5461 gaaacaacaa aaatcttttc atcgtcattc ccgcaaaagc gggaatctag aacgtaaaat
     5521 ctaaagaaac cgtttttccc gataagtttc cgtgccgaca gacctagatt cccgcctgcg
     5581 cgggaatgac gggattttag gtttctgatt tcggttttct gttttaaggg aatgacgaga
     5641 cttgagatgg cggcatttat cgggagcaac agaaaccacc ctgccgtcat tcccgcaaaa
     5701 gcgggaatct agaacccaac acggcaaaaa tttatccgaa gcgacaacaa tcttttcatc
     5761 gtcattcccg cgcaggcggg aatctagaac gtaaaatctc aagaaaccgt tttacccgat
     5821 aagtttccgc accgacagac ctagattccc gcctgcgcgg gaatgacgga ttttaggttt
     5881 ctgattttgg ttttctgttt ttgagggaat ggccgatttt gggtttctgt ttcggttttc
     5941 tattttgcaa gaatggcaaa atttcagatt gcgggcattg ttaagtattt ctatttttta
     6001 cctgccgtat ttatttccgc cccttgaagt cggcttcttc ctcgacagac acgctgttca
     6061 tctgtttgat cagcttttcc gacttctctt cgtcttcgca gcggatgact ttcacaatat
     6121 cgctttcgag ctgtccgaca ttgctgtgca gaatgatgtt tttgacgggc aggatgttgt
     6181 tggggttcat ggaaaaacgg cgcagcccca tacccaataa aacgcgggta aacgcggtat
     6241 cgcccgccat ctcgccgcat acggatacgt ctttgtccat gcggttggcg gtacggatga
     6301 cgtgttgcag cattttcagc acggcgggat ggccgggctg gtagaggtgg ctgacgctgt
     6361 cgtcgccgcg atcgacggac aagatgtatt gaatcaggtc gttggtaccg acggagatga
     6421 aatcgaccag tttcaaaata ctgccgacgg tcagcgcggc agacggaatt tcaatcatac
     6481 agccgatgcc gactttaccg aaggcatcgc cgcgttcggc aagctggcgt tgcgcggtgt
     6541 cgaggtggat gaggcactgg cgcacttcgg atacggaggt aatcatcggc cacatcatcc
     6601 gcacggggcc gtgggcggcg gcgcggagga tggcgcgcat ctgggtgcgg aacatgaccg
     6661 gttcggcaag gcacaggcgg atgccggtca tgcccagcgc ggggttgagg ctgccgttgg
     6721 gcgtgctgtt tttcccgaac cagcgcgggt ttttgtccac acccaaatcg actgtccgta
     6781 tcgttacgct tttgcctttc atttttttga caatcgcgct gtacacttcg tactgctcgt
     6841 cttcagacgg catcgtatcg cggttcaggt aaagaaactc gctgcggaac agcccgatgc
     6901 cgtctgcgcc gaggttgtgc agcggtttca cgtcttcggc ggattctata ttgcccacaa
     6961 gctcgatgca gaccccgtca gcggtggcgg cggcggtttt tttgagcttg ttcaaatcgc
     7021 gtttgtggct gcggtattcg cgggcacggc ggcggtattc gttcaacacc gactcatccg
     7081 gcgcgataat caacacgccg ttgataccgt ccacaatgac cgtttcgccc tcggtaatca
     7141 gtttgcgcgc gttgtgcagc ccgacgacgg acgggatgtc caagctcctg cccaaaatcg
     7201 ccgtatgccc ggtggggccg ccggcatcgg taacgaaggc ggcaatgcgc tgctctttaa
     7261 acaaaaccgt gtcggcgggc gaaaggtcgt ttgcaatcag aacggtttcg tcaaacaggt
     7321 tgtcggcaac ttccaactcg ttgccctgcc cgatcaggtt gttgtggatg cggcggacga
     7381 cttgcagcat atcctgcttg cgttcgcgca aataggcatc gtccatattg tcgaattggg
     7441 cggcgagttt gtcgctctgc tgcttcaatg cccactcggc gttgattttt tgttccctta
     7501 aaatatcgac gggttcgcgc gacaaggtaa catcggtcaa gagcatcagg tgcagcgaga
     7561 tgaacgcgcc caactcggtc ggggcgtttt cgggaatcgc gctgcggagc tgttccaact
     7621 ctttgcgcgt ggctttgacg gcggcatcga aacgttcggc ttcggcatcg gtgtccgcct
     7681 ccgcaacatc atactgcggc acttcctccg taccgcgcgc aatcaggtgg gcgcaaccga
     7741 cggcaatgcc tttgcccgcc gccacgccgt gcagcacgat actcattatt cgccctcgcc
     7801 gaagtagccg ttgattaagt cggtcagggc gcgcatcgct tccgcctcgt ccgcgccgtc
     7861 cgtctccagt tcgatgaccg tacccttggc ggcggcgagc atcatcagcc ccataatgct
     7921 tttgccgttg acgcggctgt cgtttttcgt aacccagact tcgcttttga attgggacgc
     7981 ggtttgggtg aacttgttgg acgcgcgggc gtggagtccg agtttgttga tgatttcgat
     8041 ggattgtttg agcatttcga ttcccgtgtt atgtatatcg gcagcagacg ccgtttaaaa
     8101 tgttttcctg ccctgccgct tcttcagacg gcatcgccgc tgcgccggca caccaaatct
     8161 tcgggcgcgg acgtgatggc gaaaatgcct tttaccgccg cctccctgac gcattcggta
     8221 aaggcggcaa ggtcttccgc cgccggcgaa tattggacgg ccttaaccat catcggcgcg
     8281 ttcagcccgg tcaaaatcgc cgatttgttt tcgcgcacga ggcggcgggc tgcattgcag
     8341 ggggtcgcgc cgaaaatatc ggtcataatt agcacgccgt cgttgtcggg aaactcctga
     8401 agcgcggcaa tggcgttgtt gatgatgtcg tcttggtctt ccgtcggttg cacgccgagt
     8461 atgcggacgt tttcaggcag tccgcccgga aaaaaatgat gtgccagctt gcggtaggct
     8521 tcgcctatgg tttcgtgtgt gatgattaaa agccctatca tattatgcgt cctgttcctc
     8581 gttgtcctac cggcgtatgg gcgcgatgcc gtctgaaagg ccttcagacg gcatcgcgtc
     8641 cttattttcc gtccaatgcg taaatctcgc ccagattgcg ccagcagccc gccgcatcca
     8701 tgccgtaacc gaaaacataa cggttcggca catccagtcc gacataatcg gctcggatag
     8761 gcttgggttt gtcaatcagc ttgttggcga acaccgccgc acggcagctt gccgcaccca
     8821 tttccaaaag tttggcttga atggcggaca tcgtatgccc ttcgtccaaa atatcgtcca
     8881 gcacgacgac gtgcctgccc cggatttgtt ccgcatcggg catacgcttc cagttgaacg
     8941 cgccgccctc cagcttgtcg ccgtaacggg aaacgtgaac ataatcaaaa tctaagggaa
     9001 aacgcaacag cggcagcaac tgccccgtaa acaccaccgc gccgcccatc acgggcagca
     9061 gcagcggata tttgccgccc aaatcgcgcg taatctcgtc cgccactttt tgcagtgcgg
     9121 cacggcattg gccttggtcg aacaaaagat cagcgttttc aagcatcgcc tgtgtttcaa
     9181 ggcgtttggt ttctaaatcg gtcatatgtc ggaatcggtc ggtaaaagga aaattataaa
     9241 ccaaagtatc ggatgccgtc tgaactgtcc tgctcagcgg tcggtacgca cgcgcacaaa
     9301 tgtggcaaat ttcggcgtgc ctttccgcgt aaagccacgg taacggtagg taatcagcgt
     9361 gccgattttg ggcgggttgt cgcggtcttt gtccttgaaa ccgctgccga tgcggaattc
     9421 gccgtgtcgg tttttgcagc cgaccgcgcc cagccgtccg gcgtttcgcc ctttgccctc
     9481 atagtgccgc gttaccgtgc attcgtcgtc gtattggctt ttcagcttca ataattggct
     9541 gctcctgccg ccgctataac gggattcggg ctggcgcagc atcacgcctt cgccgccctg
     9601 cgcttcgatt tgttttaaaa agtccatcgc gtgctgccgg tcgcgcactt tgatttgcgg
     9661 gatgatggta atcggcgcgt tcggatgcgt tttcagccac tgcgttgcga ctgccaaacg
     9721 ttggtagaga ttgccctgcg ccttgggtac gtcgaaaacg tgcagacgga tgccgcgcca
     9781 gtctgaagaa acagaacgca cggtagcgga aatctgctcg aactgaccgc gtccgctata
     9841 caattcgccg tccaaaggat aaggcggaaa ctgcgcggta aagcctttgg ggggcgtaaa
     9901 cgcgtagccc tgacggctta tcaggtgctt tccgtcccaa taggcgcgca cgccgtcgag
     9961 tttctcgctc attgcccagc cggcaatatc ctgccctttg tattcctgcg ccagcatcaa
    10021 atccgccgcg cctgccgatg cagggatgaa aaccgccgta aaaatcggta tgatgccgcc
    10081 gattgtcttc ttaatcatct gattccccaa atatcaaaac aggcggcaaa ccgccataaa
    10141 acaaacggca aacccgatgc cgtctgaaaa accgtttagg aacacgccat gaccctacgt
    10201 tacgaaatcc tctccgttac ccccttccgc caaaactgca ccctgatttg ggacgacgaa
    10261 agcggcgaag ccgtcctgac cgatgtcggc ggcgacgtgc cgttcctgct gcaagcgttg
    10321 gcaaaccgca aacttacgct cacggcaatc tggctgacgc acggccatct cgatcacgcg
    10381 ggcggcgtgg tcgaaatgtt gaaaacgcat aaagtccctg tcctcgggcc gcatccggac
    10441 gatgaattcc tgctccaatc gctgccgcaa accaccgcgc aatacggatt tcctgtctcg
    10501 cccgcctttg cgccgaaccg ttggctcgaa gaaggcgaaa cgctcacggt cggacgctat
    10561 gcctttcaag tgctgcatat tccgggccat acgccgggac atatcgtctt ttattgtgcc
    10621 gaggcggaat tgctgattgc gggcgacgtg ctgttttacg aaaccatagg cagaaccgat
    10681 tttccgcgcg gcaaccacgc cgacttaatc aataatatcc gcaacaaatt attcgccctc
    10741 cccgaaaccg tgcaagttgt cgccggacac gggcgtatga cttccatcgg acacgaaaaa
    10801 cggcacaatc cgtttttcta accgacttca ctacggtctt cagacggcgt catctgcact
    10861 gatgccgtct gaaacgcaaa aggctcagac aaccgccgcc ttgccggtgt acctcgccgg
    10921 acaaggcttt ggtaagtact tcaaacaaac cccaaatcag aaccggtaca gttacgccgc
    10981 gctttcggca ttcccgcccc tgctgaaaca agcttttccg cacaagtcag actgcttcat
    11041 cttctgccac atattccaaa gattccgaca acgccgttgt ttcattggca cgttcggtca
    11101 aatcgcgtgc aagctgcttg tttgccttca cgcccaacag acgcagtttc tcggcgcgtc
    11161 cgaccagatt cccgcgcccc tcggcaagtt gcttaaatgc cgtctgaaag ctgttttgcg
    11221 cctgatcgat gcctttgccg acgctttcga gcgtctgtac aaagccgaca aatttgtcgt
    11281 acagcttgcc gccttcgtcc gcaatcgcca gcgcgttttg gttttgctgc tcgttgcgcc
    11341 aaatattcgc caccgtccgc aaagtcgcca gcagcgtact ggggccgacc agcataatcc
    11401 gtttgtcgaa acactcttgg aacaagccag cgtcattctg caacgccaac aggtaggccg
    11461 gttcgacagg gataaacata aagacgaaat ccaatgtgtt cacaccttcc aaatcggtgt
    11521 aatccttcag cgacaagcct ttcatatgcg cgcgtatgct ggcgacatgc gccgccaact
    11581 cgcgtgccgc cgtatccgca tccgccgcct gcgtgtagcg cacataagct gtcagcgaga
    11641 ccttggagtc aatcacaatc tgcttgttgt cgggcaggtt gaccaaaacg tcgggctgga
    11701 ggcggcgcgt gccgccgtct tcctcttttc ggacggatgc tgcctgaacc acatattccc
    11761 gccctttctg aaggccggaa ttttccaaaa ccgtttccag aatcatctcg ccccaattgc
    11821 cctgaacctt attctgcgta ccggtcagcg cgttggtcag ggcctttgcc tcgctgtgca
    11881 gctgcgcatt caatccttga agccgtttca attcgttttc caacgtcagc cgctcgcgcg
    11941 attctttatc ataggtttgc ttgaccaact cgccgaaacc ttggatgcgt tcgtttagcg
    12001 ggttcaaaac ctgatggagc tgctcccggt tctgctcggt aaaacggcgg cttttttctt
    12061 ccaaaatcgt gttggcaaga ttttgaaact gatcgctcaa acttttgcgc gcctcgccca
    12121 gcaaggacag cttctcttca gaagcaaggc gttcctgttc gatttgcgtt gccaaacgtt
    12181 cgttttcaac cgccaaaccc tgtgcctttt cctgcaactc ggtatgcgac tgcctcaacc
    12241 gctccgcttc cgcctctttt tcctgcaaat gggcaatctg tttttcggct gcggcaaaac
    12301 ggttgccgac atcggcaagg tcgttttgca cgtcgcggac agtttggcgg ctttcttcca
    12361 aatcggtttc gattctttgg cggatttggc gttccaaggc atattggtcg gcaaacgcct
    12421 tccgttcctg tccgagctgc gttgccaaac gttcgttttc aaccgccaaa ccctgtgcct
    12481 tttcctgcaa ctcgatatac gactgcttca gccgaaccga ttccgcctct ttttcctgca
    12541 aatgggcaat ctgcttttcg gctgcggaaa aacggttgcc caaagcataa ttttcgtcct
    12601 gcaaatgtcg gtatttcccg tccaaaacgg ccaattccga tgcaatttct gcgtgtgcct
    12661 gttcgacaaa atcacatctt gccgcctttt ccgccagttg cgcgttcaaa tcggcaaact
    12721 cgccctgaaa cctgcccttc atcagcaacc atgtaaacaa cacgcccgac accaacgccg
    12781 ccaaaggcag caaaacagtc atcagttcca tcaatcatcc taatattcaa acattttcac
    12841 accggacaaa accgccgctt attccgattc tacctgtttg ttcgacatag ctccaaaaaa
    12901 tatagcggat tggctttaaa cctgttcgac atcgccttac catgctgctt gcggtttcag
    12961 accttttcct aattcaatat caatctgcca caaaccctga ttaagttccc gatgtctgac
    13021 atttttagaa tgatgccgtc tgaaatgttg cagctatgtt cagacggcat acggattcag
    13081 gcttttcaaa cggcaggcaa aatgaaaaaa gggcaaaccc taaaggattt gcccttttgt
    13141 tccaaacgct tagtgtacgt ctttccaata ttcttttttc aggaaatacg ccaaaggcag
    13201 cataaccgca aataggaaaa tcatcacgac atagcctata cgtttgcgtt gcagttgtgc
    13261 aggttcgccc atgtacacaa ggtaattgac caaatcgcgt acatatgcgt cgtactcttt
    13321 ttggatcact ttgccgttag gcagacggcg gctgtgcaaa ccggtagatt cccaatacag
    13381 cttaggcttc atctcgccgt gttcgtcttt taccataacc ggctgacctt tggcatccaa
    13441 ctcaacggct tgaacgcctt gctgttccca caacgggtgg ggcataccga ctttatcgaa
    13501 tacagtattg ttccagccgc tcggacgggt cggatcttta tagaagccgc gcatataagc
    13561 gtaaaggtag tctgcacctt tggaacgcgc aatcaatgtc aaatcgggcg gagcagcacc
    13621 aaaccatttt gccgcatctt tcgggttcat cgccgaatgc atgacatcgc cgacattatc
    13681 ggtggtaaac atcaggtttt tcttgatttc ttcgtcagtc aaaccgatgt ctttcaaacg
    13741 gttgaaacgc ataccgcttg ccgagtggca ggacaggcaa tagttggtaa agatttgtgc
    13801 gccgcgctgc aggctgactt ggtcgcgcaa atcgatatcg actttctcat agtgtgcatg
    13861 accgcccgaa gcaaatgccg cacccatggg tactgccaac agcaaggcag caaaccaatt
    13921 tttcatagct tgtttcattt tgactgccct catcagatgt tggttgcaaa caggtaagca
    13981 cccacggcgg taatgccgac gtaaacaaag aacatgattt tttgtttggc ggtgctcatg
    14041 gttacgcgtt cgggaaccgg tttgttttta tccagcttgg tatagaacgg catacccagg
    14101 aagaatgcga aatagacgaa ggacaggatg cgcgcgacca aagtacgcgt atcggttgcc
    14161 accatcgcgc ccaaaatacc caaaccgata aaggaaatga tgaacaagac caatgcggtt
    14221 ttgaaaatcg ggccgcggta acggacggat ttcacctcgc ccctatccag ccaaggcagc
    14281 agggcaatca acacgaccgc cgcgcccata ccgattacac cccaaacctg cgttcccaag
    14341 aacgagggaa tcgcacgcaa aatggcgtag aacggtgtga aataccatac cggcgcaatg
    14401 tgcggaggtg ttttcagcgc atttgccgca tcgaagttcg gcgcttcgag gaagtagccg
    14461 ccgccttccg gtgcgaagaa caatacggca cagaagacaa tcaggaatac aaccacaccc
    14521 aagacgtctt taacggtata gtaaggatgg aatgggatac cgtcgcgcgg gataccgttt
    14581 tcgtctttgt ttttcttgat ttcaacgccg tcagggttgt tagaacccac ttcgtgcagg
    14641 gcgatgatgt gcgccacaac caagccgagc aataccagag gtaccgcgat aacgtgcagg
    14701 gcaaagaagc ggttcagggt aacgtcggaa acgttgaagt cgccgcgaat ccaagtggac
    14761 aaatccggac cgataacagg gatggcggag aacaggttaa taattacctg cgcaccccaa
    14821 aaggacatct gaccccaagg cagcaggtag cccataaagg cttccgccat caatgccaag
    14881 aaaatcagag aaccgaaaat ccacaccaac tcacggggtt ttttgtacga accgtaaatc
    14941 aaaccacgga acatgtgcag ataaacaaca atgaagaaga aagatgcgcc ggtggaatgc
    15001 atgtagcgga taatccaacc gccggacacg tcgcgcatga tgtactcgac tgcagtaaag
    15061 gcagcaggca gatggtaggc gttcaggttg ccgtcgggtt tgtagttcat ggtcaggaaa
    15121 atgccgctga cgatttgaat cacgaggaca agcagggcca aagagccgaa gtagtaccaa
    15181 aaattaaagt ttttcggtgc gtagtattga gccaaatgct cattccacat tttagacagg
    15241 gggaaacgag cgtccatcca gcctaacaat gcttttgctt tgctattggt ttggtttgcc
    15301 ataattatcg ttccttattc ttagtcttcg cccaccaaga tagttgtgtc gctcaagtat
    15361 ttatatggcg ggacaaccag gttggtcggg gcaggaacac ctttatatac gcggccggcc
    15421 aagtcgaatt tcgaaccgtg gcaggggcag aagaagccgc ctttccagtc tgcacccaaa
    15481 tcggcagggg caatgtcggg acggaaggtg ggcgagcagc ccaaatgggt gcagataccg
    15541 atggcgacaa ggatgttcgg cttaatcgaa cgggtctcgt ttttagcata ctccggctgc
    15601 tgttccgcat cggaattggg atcggtaagt tcgccgttca ggcctttcag gtctttaagc
    15661 tgctgatctg tacggttgag cacccaaatc ggtttgcctt gccactcggc ggtcagcaac
    15721 tgacccgctt cgattttact gacatccacc tcgacggcag caccggcggc cttggctttt
    15781 tccgaaggga aaaaactggc cacaaacggc gttgccacac ccaatgctgc cactccgccc
    15841 gcgccgcagg tcgcgagtgt caggaaacgg cggcggccgt tgttgatttc ttgattatcc
    15901 attattcagt cgtcctaata ttttgggaat accgagccat taaacgttgc aattttaccc
    15961 agtttgcagt gatactcaaa gcattattta aaataaggta aagttttatg atatttctca
    16021 agactcaagc cggattgttt tcgtcaaaat ggcacacttc caacccgaaa acctctgccg
    16081 ccgattctgc cagcgcgcgt acgccgtaac gttccgtcgc gtgatgccct gccgaaatga
    16141 aagccgtacc cgtttcattg gcaaggtggt attgagcttc agagatttcc cccgtcaaat
    16201 acagatcgac accttcgtct attgccgtct gaaaaaaccc ctgcgccccg ccgctacacc
    16261 atgcaacccg tcggatttcg cgttcgggtt tgccgatgac gacaggcttg cgttgcaaaa
    16321 ctgtttcaat atgcgccgcc aatgcgccga gtgtcttggc ttgtttcagg ctgcccgagt
    16381 tgagcaggtt ttgttcgccg aaccgttttt ctgtcgcaaa acccaatctg tcggcgagtt
    16441 gggcattgtt gcccagtgtg ggatgtgcat ccaggggcag atggtagcct gccatattga
    16501 tgtcgtgccg taacagtgcg gcaatccgtt cttttttcca accagtaacg gtcggcaact
    16561 cgtttttcca gaacataccg tgatgtacca aaagcaaatc tgccttctgc tccacagcaa
    16621 aatcaatcgc tgccctgctt gccgttaccg acgtaacgat tttcccgata tattccctcc
    16681 cttcaacctg caaaccgtta ggggcgtaat ctttaaacaa cgctgtctgc aatgtttcat
    16741 tacaccaagt cagaaaatcc ctgcgcaata ccatcttttt tcctaatcgc tttaaacaag
    16801 cgggcattct aatcgcaaaa tgtccggaat tcacattttt ccgatttgca cccgcatatg
    16861 aattatttta atatgcgccg gttcaatatg ccgtctgaag ccccatggat tccattatcg
    16921 aattgcgcca cctcaaaacc ctgctggcac ttgaagaaac cggcagcgtc tcccttgccg
    16981 ccaaacgggt tttccttacc caatccgccc tttcccacca gatccgtatg ctcgaaaacc
    17041 actacggcac gccgctgttc gaacgcaaat ccacgccctt gcgctttacc ccggtgggcg
    17101 aaaggctgct gcgcctcgcc cacgaactga tacctcaagt tgctgttgca gaacgagatt
    17161 tggcgcgaat cacagaaggg gaggcgggag agctgcggat tgccgtcgaa tgccatacct
    17221 gtttcgactg gctgatgccc gccatgggcg aattccgccc gatgtggccc caagtcgaat
    17281 tggatatcgt atcgggattc caagcggatc ccgtcggact gctgctgcaa caccgtgccg
    17341 accttgccat tgtttccgaa gcggaaaaac aaaacggtat ttctttccaa ccgctgtttg
    17401 cctacgaaat ggtcggcatt tgcgcaccag accatccgct tgccgccaaa aacgtttgga
    17461 cggcggaaga ctttatcggg gaaaccctga ttacttatcc cgttcccgac gagatgctgg
    17521 atttgcccaa aaaaatcctg attccgaaaa acatcaaccc gccgcgccga cacagcgaac
    17581 tgaccatcgc cattatccaa ctggttgcca gcagacgtgg cattgccgcc cttccctatt
    17641 ggacagtcat gccctacctt gaaaaaggct atgtcgttca ccggcagatt accgacgacg
    17701 gactgcaaag caaactgtat gctgccatcc gcactgaaga tacggacaag agctatctga
    17761 acaacttttg ccaaatcata cgtgaacgcg gttttgcaga tttgcccgga ctgagcgaac
    17821 tggaatcggt ctgacccctt attcaaccat acccggcagt ttttctattt tttcatgtat
    17881 agtggattaa caaaaaccag tacagcgttg cctcgccttg ccatactatt tgtactgtct
    17941 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tactgttttt gatttttgcc
    18001 caatctgtaa tctttagatt gccaatgggg aaccgtctac tacaaataaa aaaccctgcg
    18061 ataagcaggg ttttttgaat ttccaacatt aacgtttgga gaattgtttt gcacggcgtg
    18121 ctttgcgcag acccggtttt ttacgttcga cttcgcgggc atcgcgggta acaaaaccag
    18181 cttgagacaa ggcgggtttc aacgcggcat cgaagtcgat cagggcacgg gtaatgccgt
    18241 ggcggattgc gccggactgg ccggtttcgc cgccgccaac aacattgact ttgatgtcga
    18301 aagattcggc gttttcagtc agaaccaagg gttggcgaac aaccattcgg ctggtttccc
    18361 gtgcgaagaa ttcgtcaacg ggacgaccgt ttacgatgat ttgacctgta cctttaatca
    18421 ggaatacacg agccactgaa cttttgcggc ggcctgtgcc gtagtagtat ttaccgttca
    18481 tgtcgcgtcc ttatttcagt tccaaaactt tgggttgttg cgcagcatgg gcgtgttccg
    18541 cacccgcata cactttcagt tttttaatca tggcgtaacc cagaggacct ttgggcagca
    18601 taccttttac agcttgttcc aaagcgcggc ccgggaattg ctcttgcatt tcgcggaagg
    18661 tgcgttcgta gataccgcct gggaaaccgg aatggcggaa gtatttttta tcttcgaatt
    18721 tggcaccggt tacacgcagt ttgtccgcat tgataacaat gatgtaatcg ccggtatcga
    18781 cgtggggggt gtattcaggt ttgtgtttgc cacgcagacg gctggcgact tcggccgcaa
    18841 cgcgacccaa gactttgtct tgggcatcga tgacgaacca ttcgcgcttc acctcgtggg
    18901 gtttcgctga aaaggttttc atagtggaaa tccagataga tatagaaagt tgtaaatttt
    18961 aaagacagga ttcgattttg tcaatcgcat taccgcgtta cggaaggatt ttccggattt
    19021 cggcagactg catactgctt tttcgggcgg ggcggcggcc aatgtgaaaa accgcatcgt
    19081 tgcgatgcgg ttttgaatgg gaatccccgc gagagccgtt tcggccgaat ccgcttgaac
    19141 cttgctgaca aggcggctgc ctcgggtagt ttcgggtgcg tccgcaaaag gacgctcgcg
    19201 cccactactg ctcccggcaa ccttaagcga acttattggt tcaaaggaat atatgccttc
    19261 gcggacaccg cagggaaaaa ggggttattc ctgcgccaag cgggatagtg ctttttggca
    19321 ggcgttgtcc atatcggcta ttttacgcgc aaaatcgccg attgccaaat cgccgccgtt
    19381 cagggaggtt ttcaacaggt cgtggacgac gttgagcgcg gccataatga cgattttttc
    19441 gctgtccgcg acgcgtccgc cttcgcggat ggcttcggct ttgccgttga gcattccgac
    19501 tgcctgcaac agtgtgtctt tttcttctgc cggcgtgttg acggtcagcc gggcgtgcat
    19561 gacttcgatg tggacttgtt cgatgttcat cctttaatcc ttattgctgc gtttcctgcc
    19621 gttgggggag gcgcgctgcc agtgcgctga ttttttccct gctctgttcg agcaggctgc
    19681 ggtatcgtgt attttcttct gtcaggctgt caattttgtt ttgcaggtct tctttgagtt
    19741 tgccgacttg gacgagcagg gcttcgctaa gttcgtcgac ggcggtttcg tgttcgagtt
    19801 tttgccgctc gtgcgcccgt ttgagttcgg cgacggtttc tttgaggcgg cggttttcgc
    19861 tgacgagggt ttcgaatttt tgtaccagcg tataaacgct gctttcgagt ttttcgatat
    19921 tttgtttcat aaccttacct gtccgtatgc cgtctgaagg cttcagacgg catctgtctg
    19981 ttgtttattc aaaacgcgcg ctacgttcca tcagcctttc gacgacctgc tgaggggtca
    20041 tttccttacg gatgagttgc agcagggttt gggtaatcgg catatcgatt tggtacttac
    20101 aggcagtatt gaagacttct tctatcgtgc tgaccccttc ggaaacgtgt ccgatttcga
    20161 ccagcacctg atgcagttcc ttgccttctg ccaaacccaa gccgacgcgg cggttgcgtg
    20221 agagtgcgcc agtgcaggtc aagatgaggt cgccgatgcc tgccagcccc atcatggttt
    20281 tgggctgtgc gcccattgcg gaggcaaggc gggtaatttc agctaatccg cgcgtaacca
    20341 gtgcggcacg ggcgttaagc ccgtactcta ggccatcgga caatccggtg gcaatcgcca
    20401 taacattttt taccgcgccg ccaaccgcca cgccgataac gtcggtactg ccgtaaagcc
    20461 tcatgacagt cgtgttgagc tgcggtacga gttcttcaat ccactcttgg ttttcggagg
    20521 cgaggacgac ggcacagggc agttgtttgg cgagttcctg tgcaaaactc gggccggaga
    20581 gtacgccgat tttcttattg tcgggcaata cttctttcaa tacttgaaag gtcagcagcc
    20641 ctgtatcctg ctcgaatcct ttgcaggcgg cgaggacggg gaggtgtccc gcgccgtact
    20701 gtttgagcag ctctgcgctg cttctcaatc cggcaacgga ggttacgata aggacaagtc
    20761 cgctgtcttt gagcgcgtct gccaaatccg cacacacttc caaggtttcg ggaaaggaaa
    20821 agccgggcag tccgcgtttg ttttcacgcg cttcctgcat ttggcggact tggtctgcgt
    20881 tgcgcgtcca cagggatacg cggttgccgt gttgggaaaa gtgcagggcg agcgccgtac
    20941 cccacgaacc tgcgccgata acggtaattt tcattggtcg tctttcaaca tatcactgcc
    21001 gttcacttta aaacaatcgg tgtttctctg caagtgcggt cagggaaatg ccgtctgaaa
    21061 ggtgttcaga cggcattttg ccccgatgcg gcacaatcag cctgtattgc gcaaaccttg
    21121 cgccacgccg ttgatggtca ggtgaaccat cagcagcgcg tgcggattgt cgggttcttt
    21181 acgcaggcgt ttgagcatgg cgacctgcag gccgttgagc gcgttcaggt agggaatcct
    21241 caaggcaagc gaacgggcga ggctgcggtt gtcgcgtaaa agttcttcgg tttgcagcag
    21301 gtcgagcagg gctttgcggc tgcgttggta ttcttcctta atcatgccga agatgacggc
    21361 ggctttttcc ggcgattcgc tcaagccggc atagttttcc gcgagggtga tgtcggtttt
    21421 cgccatcact tgctccatat tggaaagcat ggcttggaag aacgggttgc tttgggcgtg
    21481 ttcgcgcagg gcggcgagcg tttcgggttt gtcttcgcac aaggtttcca ccgcgctgcc
    21541 gaaaccgtac caagcaggca gcatgaggcg gttttgcatc caggaaaata cccacggaat
    21601 cgcgcgcaag tcttgaatcc gcgctaaggt cttgcggctg gcaggacggc tgccgaggtt
    21661 gagggtggcg atttcctgaa tcgggctggt ttgcaggaag tagtcgatga agtcggggtg
    21721 ggtaatcagt tcgcggtagt atttgaacga tacgtccgac aatgcctgca tcagtttggc
    21781 atcagggtct tttttatccg gcaggatgct ggcttccaaa gtcgcggcaa ccaaggtttc
    21841 caagttgcgt tgggcattgc cggggtcggc gtatttggcg gtaatgactt cgccttgttc
    21901 ggtaatgcgg atttgtcccg ccacgctgcc cgccggttgg gcgagaatgg cttggtaaga
    21961 cgggccgccg ccgcgtccga cgctgccgcc gcgaccgtgg aacaggcgca tgcggacatc
    22021 gtattttttg aagagttcga ccaagcccaa ttccgcctga tacagacacc atgagctggt
    22081 aacatagccg ccgtctttgt tggagtcgga gtagccgagc atgatttctt ggatgtttcc
    22141 gcggctttca agcagggcat cataccaatc gaggcggaac atggtttcca tgaccggaca
    22201 ggcgttttcg agtgcttcga tggtttcaaa cagcggcacg atattgatgc ggctgtgcgg
    22261 tttgccgttt tccaccgcca acaggccgct ttctttcagc agcaatgcca aggcgagcag
    22321 gtcgctgggt tgttcgcagt tggaaataat gctttgtgta acggcatctt cgccgaattc
    22381 gtctttgatt ttgcgcgctt cgttgaaaat cgccagttcg cggcgggtat ggtcgctgta
    22441 tgtgataaac gggctgtaca gcggacgttg atggctcaat tcgcgcaaca gggcggtttg
    22501 tttttgctct tcgttcaggc ggttgtagtc ttccaagcct gcgtgttgga aaagctcggc
    22561 aaccacatcg acgtgtttgc ctgcgtgttg gcgcaggtcg agcggcatca tgtggaagcc
    22621 gaacacggac acggagcgga tgaggtctgc caaacggcct tcagcaagca ggcggctgcc
    22681 gttgtcgata agggaacgtt gcaatttttt caaatcatcg agaaattctt gtgccgaagc
    22741 atacggctcg agaaagccga atttgcagcc catgcccaaa ccgagcgcgc gcgctttgcc
    22801 catagcgcgc gccatgatgt aagcaatggc gcggcggtag ggttcttcgg tgcgagcgat
    22861 ttcttcgtca ggcgagaggg ctgccagtgc cattacgtcg tcgttgactt tgacgcggcg
    22921 gatggagagc ggcagttcgc ggtaaagttt gtcgagttcg ccacggtaaa aacggaacac
    22981 ggcatcggcg tggcggcgga aggcaaaacg caaggtttcg gcagaaacga acggattgcc
    23041 gtcgcggtcg ccgccaatcc agccaccgat tttgaggata ttcggaacgc agacatcggg
    23101 atagaccatc tgaaagtcgt gttccatatt gcggtagagc ttgggcaggg cttcgaaaaa
    23161 gctcatcggg aagatggaca cgccgttgtt gatttcgtcg ttgacgctga gtttgtggcg
    23221 gcgcgtttcg ctggtctgcc acaagcccag caggatagtg tcgatttcgc ggcgcagccg
    23281 tgccagcgcg tcggcattgg tgcagcgttc gcgttgcggc aacagtgcgc ggatgcggcg
    23341 gttgaagctt aagacggttt ggcgttgcac ttcggtcggg tgcgcggtca aaacggcggt
    23401 aacggacgta ttgtccaact gccgctgcac cgatttgccg tcggttttcc ccgctttgag
    23461 cctgcggacg gtttccgtca ggctgccttc cgcgccgccg cgtccggctt cttcgtggat
    23521 ttggcggcgg cgttcgtggt gcacgtcttc ggcgatgttc aaaatctggg cgaacaggcc
    23581 gcaggccaag gttaaatcgt gggtttgttg ttcgtccaat tgcggcaata ctttttcaat
    23641 caatgccgcg ctgtcgtcgg aagtggacaa gagtttgacc gtttcgacaa ccaacggcga
    23701 ggcttcttcg tgcaggaggt tgaacaggga ttgtttcaga aattccgcgt ccgccgccaa
    23761 agccgcgtcc ttcggattgt tcagaatatg cagttgcatg atttttctct cattgccgta
    23821 aatactgtaa atgtacctca aatgccgcat ccgtgccaaa ccgttcacac tttaaccgcc
    23881 cgtgtcccga aatgccgtct gaagttgaac gccgcccgac ggcagcgtta caatcgcccg
    23941 caactgtttt ttccgaacat catcatgacc acgaccgaac acgacaacga cgacgaactc
    24001 ctgctgcggt acagccgcca catcctcttg gacgaaatcg gcatcgaagg gcagcaaaag
    24061 ctttccgacg cgcatatttt ggtcgtcgga tgcggcggtt tgggtgccgc cgcactgccc
    24121 taccttgccg cttcgggtgt cggcacgctg accatagccg atttcgacac ggtcgaactg
    24181 cacaacctgc aacgccaagt cgcatttgac gagggcgatg tcggcaaact caaaaccgaa
    24241 gccttggcag gccgcctgaa acgcatcaac cataccgtca acgtccgcgc cgtcaacgaa
    24301 aaactcgacg gctgccgcct gaccggtttg gttcaagccg ccgacatcgt tttagactgt
    24361 tgcgacaact acgccacgcg gcaagccgtc aaccgtgcct gcgtgcaaac gaaaacaccg
    24421 ctggtttcag gggcggcggt acgctttgaa gggcaacttg ccgtgtaccg tcccgacttg
    24481 cccgactcgc cgtgttacgc ctgcctgttt gacggcggat cggcttcaga cggcatctgt
    24541 tctctcttcg gcgtgttctc gccgctggtc ggcatcatcg gcagtaccca agcggcggag
    24601 gctctgaaaa tcctgctgga tgcgggcgaa ccgtcgcacg gcaggctggc ggtttaccgt
    24661 gccttggaag ggggctggca atatttcgac ctgccgcgca accctgaatg cccggtttgc
    24721 ggcacggcgc gataaaccct gccgccgttt cagacggcat ccaaacggat gcggcggaac
    24781 ggttttaaaa atttaaaaat ttacatttct ttgcaaaaaa aaaaaaaaaa aaaaaaaaat
    24841 aaacttacct tataattgca attgttttag caatgtctgt ttcgcagact cattgagtaa
    24901 aacgttttcc ccgtaatgtg tttggccgtc tgtccccttt gggttcggac ggcttttttt
    24961 tggctgtgtt tgaatacccg gttggtttta tctgtttgca gcgggggaag ccgcttattt
    25021 ccgttcgggc ggaaaacggt tccatcggat aaaaggcatt ttgtccgact gattaaa
//
LOCUS       DAWFIT010000022        24773 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFIT010000022 DAWFIT010000000
VERSION     DAWFIT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.022"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGQ85_000851"
     CDS             complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGQ85_000851"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0750311.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKLEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTTLDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPTSPKQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACCGDTEAQMDMLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSDELLSHYGNVRKLWKEVFGEEAATA"
     gene            complement(3350..3541)
                     /locus_tag="WGQ85_000852"
     CDS             complement(3350..3541)
                     /locus_tag="WGQ85_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750312.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLETFAIT"
     gene            complement(3678..4151)
                     /locus_tag="WGQ85_000853"
     CDS             complement(3678..4151)
                     /locus_tag="WGQ85_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0750313.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASEMRGYSPKNWLYVLLSDVFHRKEEFDDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4204..4485)
                     /locus_tag="WGQ85_000854"
     CDS             complement(4204..4485)
                     /locus_tag="WGQ85_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750314.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4572..4895)
                     /locus_tag="WGQ85_000855"
     CDS             complement(4572..4895)
                     /locus_tag="WGQ85_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750315.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5228..6235)
                     /locus_tag="WGQ85_000856"
     CDS             complement(5228..6235)
                     /locus_tag="WGQ85_000856"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0750316.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6467..6703
                     /gene="acpP"
                     /locus_tag="WGQ85_000857"
     CDS             6467..6703
                     /gene="acpP"
                     /locus_tag="WGQ85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0750317.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6859..8106
                     /gene="fabF"
                     /locus_tag="WGQ85_000858"
     CDS             6859..8106
                     /gene="fabF"
                     /locus_tag="WGQ85_000858"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0750318.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8461..9594
                     /locus_tag="WGQ85_000859"
     CDS             8461..9594
                     /locus_tag="WGQ85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0750319.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGGDLERLAARDIIRFPGFVNNVSEVIKE
                     HHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILA
                     EKMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
     gene            complement(10289..11139)
                     /locus_tag="WGQ85_000860"
                     /pseudo
     CDS             complement(10289..11139)
                     /locus_tag="WGQ85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
     gene            11488..13002
                     /gene="katA"
                     /locus_tag="WGQ85_000861"
     CDS             11488..13002
                     /gene="katA"
                     /locus_tag="WGQ85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0750320.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            13171..13351
                     /locus_tag="WGQ85_000862"
                     /pseudo
     CDS             13171..13351
                     /locus_tag="WGQ85_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220242.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14345..14773
                     /locus_tag="WGQ85_000863"
     CDS             14345..14773
                     /locus_tag="WGQ85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0750321.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSDKGTFPKNNASAGIASASEITGKYVAKVEVSGATITSTMKSQG
                     VNKDISGATLVLEGKQNSGSFEWTCKAGSVKAKFLPSICR"
     gene            15230..15730
                     /locus_tag="WGQ85_000864"
     CDS             15230..15730
                     /locus_tag="WGQ85_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750322.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            16206..16772
                     /locus_tag="WGQ85_000865"
     CDS             16206..16772
                     /locus_tag="WGQ85_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0750323.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(17048..19084)
                     /locus_tag="WGQ85_000866"
     CDS             complement(17048..19084)
                     /locus_tag="WGQ85_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0750324.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(19172..20044)
                     /locus_tag="WGQ85_000867"
     CDS             complement(19172..20044)
                     /locus_tag="WGQ85_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0750325.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPADSETL"
     gene            20314..22704
                     /gene="gyrB"
                     /locus_tag="WGQ85_000868"
     CDS             20314..22704
                     /gene="gyrB"
                     /locus_tag="WGQ85_000868"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0750326.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            22803..24188
                     /locus_tag="WGQ85_000869"
     CDS             22803..24188
                     /locus_tag="WGQ85_000869"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0750327.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(24259..24357)
                     /locus_tag="WGQ85_000870"
     CDS             complement(24259..24357)
                     /locus_tag="WGQ85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0750328.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(24402..>24773)
                     /locus_tag="WGQ85_000871"
     CDS             complement(24402..>24773)
                     /locus_tag="WGQ85_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750329.1"
                     /translation="VKEYQPEVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYDLVQTEYRRFKADKT
                     ENRNHKANSTFEYLHILEKTF"
BASE COUNT         5921 a         6620 c         6505 g         5727 t
ORIGIN      
        1 ccgtctaaaa agtagaatgc gctattttaa tggaaacggc ggggttttaa acggttctta
       61 tttgtttgtt ttgatttttg cttgggtaac aaatcccatc attcccacaa aaaaaatccc
      121 gtcattcccg cgaaagagga atccggtttt tgggtttcag ccattcccga taaatcgctt
      181 tagctctgcc gcaaccttaa aacggcacgg tacgaaaatg ccgtctgaaa cccagattgt
      241 caggcttcag acggcatttt ttgttcaggc ggttgccgct tcctctccga acacttcctt
      301 ccacaatttc ctgacattgc cgtaatggga cagtaattcg tcgcttactt cggttttttc
      361 cgcgtcgcgc agtttggtgt tgtgctgctg ccggcggtaa aaacggtagg cggtgcggct
      421 ttgtccggca agcgttttat caatcaaacc gcagtcggcg gcgatattca agagggcgat
      481 gttgccgtag ttgtccaaga gttgcggata ctggcgggca tgggcaagta tcagatattg
      541 gacgataaat tcaacatcga ccacgccgcc gcgtgcgtat ttgacgttgc tgtcggcagg
      601 cggatgggtg gggaacattt tttcgcgcat ttcgatgatt tcgcctgcca aggcggtttg
      661 gtcgcgttcg gcggtaagga tttcggtgcg gatgcggtcg aatgccgtct gaatttcggg
      721 tgtgccgcag atgaagcggg cgcgggtgag ggactggtgt tcccatgtcc atgcgttttc
      781 gcgctggtat ttttcgaagg cggcgatgct gtgggcgagg aagccggcgt cgccgttggg
      841 gcgcaggcgc aggtcggttt cgtagaggct gcctgcgccg gtggcggcgg aaagccagtt
      901 ggtcaggcgg cgggcgaggc ggctgtacac gtcgcctgcg tcggggtggg gatcgtcgta
      961 gagatagacg aggtcgaggt cggaggcgta gccgagttct ttgccgccga gtttgccgta
     1021 gccgatgacg gcgaattgcg gggtgtcgcg gtgttttttg ggcatatccg cccacgcgca
     1081 cgaaagggcg gcggcaatga cggtgtcggc gagggcggaa agctggtcgg agagggattc
     1141 taccgtccac aatccggcaa ggtcttggac ggcgaggcgg aagacttggg cgtgctggaa
     1201 gcggcgcagc atgtccattt gcgcttcggt gtcgccgcag caggctttga tgccgtctga
     1261 aagttcggcg gcgagtttgt cccaatcaaa cgccgtatct aataattgcg cgctgatgag
     1321 ttcgtccagc agaatcggat atttgttcag atacgccgcc acccacgagc tttggcccat
     1381 aatctctgcc agttgcgcca gggtttgcgg atgttcgttg aggaaggcga gataggcgga
     1441 tcggcggctg atgttttcga gaaaatccaa cagccgcatc aatgtatcgg tcgggttgct
     1501 ttgcgctgcc gccgcctgta cgaacagcgg cacaaccgca tcgaaacgcg gctgggcgtg
     1561 tgcggaaaga tggcggtatt tatggccgtg gcggatttgg tcgagccttg cggcgacggt
     1621 ttcggcatcg aacccgtgcg ccttcagacg gcatcgccgc ccttcttcgt cgggtttgtc
     1681 ctgccatgcc cattgccatt cgctgttgtc ttgcgtttgc tcttcgggtt cgctcaaaat
     1741 ttcgttgaac aactgattga ctttgttccg atgaacattg agaccgtctg aaaaagcgga
     1801 ataactgtcg aaacccatgc tttcggcgag cagttgccgc tgtttgggcg aggtcggcag
     1861 ggtttgggtt tgccgatcgt cccagtattg caggcggtgt tcgacatcgc gcaggaatcg
     1921 gtaggcggca agcagagttt cgacgttttc agacggcatg atcccggttt cggcaagttt
     1981 cttcagcgtt tcctgcgtgc ctttcagttg cagcgcacgc atttggccgc cgcgtatcat
     2041 ctggaaaatc tgggcgataa attcgacttc gcggatgccg cccgcgccga gtttgatgtt
     2101 gtccgccatg ccttttttgc tgacttcgct gcggatttgg cggtgcagct tacgcatcgc
     2161 ctcatacgcg ccgtaatcca gatatttgcg gaacacaaag gggcgcacca gtgctttgat
     2221 gtcgttcgga tacggcgtaa ccacgcgacc tttgcaccac gcgtagcgtt cccattctcg
     2281 cccctgtgta atcaaatatt gctccagcgc ggtttcgctc aataccaacg cgcccgaatc
     2341 gccgtccggc cgcagccgca tatcgacgcg gaacacctgc ccgtcggcgg tgatgtcgtt
     2401 cagcagcgca atcagtttct gcccgacttt ggtgaaaaac tcctgattgc cccgttcgcg
     2461 cctgccgtcg gtgtcgcccg attcgggata gacgaaaatc aaatcgatgt cggaagacac
     2521 gttcaactca tagccgcccg ccttgcccat cgccaccacg ctcaaatgct gcggcgattt
     2581 ggtataacgc ccgatcggcg tgccgtacat gtcccgataa taggcgtagg cgaaatccag
     2641 cgtggtattg acggcaaaat cggcaaacag cgtaatcgtg cgggttactt cgttcaaatc
     2701 gctgatgcgg tttatatcgc gcacgataat ctgcgacacc acataacggc gcaacccgcg
     2761 caactgccgc gccaattctt cctcgttttc ttccgcgcgg attttgtccc agtcggcaaa
     2821 ggcttggaaa tccgcttcgg tcaacacctt gtccagcatg ggcaggaaga tttcaagctt
     2881 gagtttgccg ttgtcgagct ggcgggcgag gaagagggaa tggcggcggg cggtgtcgag
     2941 gcggttgtcg gacatttcgg attccgtttg gaaggatggc aggaagatga tggcggattc
     3001 acttcaaatc aggacagggc gaggggatgc cgtaccggtt taaagtcaag ccactatatc
     3061 atagcaacct tcgatgccgt ctgaaacctt gccccgacat ccagacggat gcctgccgat
     3121 aaaaaacagc ggcaaatgcc gccgtttcct gttcacacct cgccacgctc ttttaaaata
     3181 gcataagccg agacctttgc aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg
     3241 ccaaaaaatt ttcaattgcc taaaaccttc ctaatattga gcaaaaagta ggaaaaatca
     3301 gaaaagtttt gcattttgaa aatgagattg agcataaaat tttagtaacc tatgttattg
     3361 caaaggtctc aagccgcact tttctcccgt gtggaatttt taaaaaacca tcactcttga
     3421 cgatttcatg cagctcctca cttgatttct ccgcatattc cattttcaac gccttatgct
     3481 cattgccggc ttcaatcatt ccctctccca ctgcttttgc cacaccattc caaaaaccca
     3541 taatcatgtc ctccaatcgt ttcaaataaa taaggtcgtt gtttctgtca agaaacaacg
     3601 acagtttata aactgtcgga aaacaaaatg ccgtctgaag gccggataat gcttcagacg
     3661 gcatcggatt gccggtttta accctgccct ccgccgttgt tcaaatagtg ttcccagtga
     3721 gaatataacc gggcaatatt ttcttcatag gtgtgtgcag aaggaatata accgtctttg
     3781 ggcggcatat acctgacaaa tgattctatt tcttctggat aatcaaaatc tgcataaatt
     3841 ttttcaactt cccccaaagg gtcgtcaaat tcttcctttc tatggaatac atcgcttaag
     3901 aggacgtaca gccaattttt aggggaataa cctctcattt ccgaagccaa atcttccaaa
     3961 acaggcttta attcaaaaat attggatttg ttggttaaag atagtttgaa cacacgttca
     4021 tcatgatctg aaagcgtcat ttttcgggca taagctgcca catcagccca acccaagtat
     4081 ttattcccat acccccacag aatatccttc caagaaagac ggactttttg cgcggttaaa
     4141 tcaaggttca ttttttactc cgattgcctg ccccgtcatt ccgccccttc cgattgacgc
     4201 ctgttacgaa aagtcttcca aatgacggct gattgcctct tcaacagtcg gatgccaatc
     4261 cataaatgaa gcctgcaatt cctctaaaac caactcattg aataaatcat ccaaattccg
     4321 ccaatccgtt ttttcatccg gatagatgaa atttgccata acgacagtat gtttgttttg
     4381 ggaataatcc gataaggcgc aaatcatcag ataccgcccc gaatgctcca gataaaaaat
     4441 tcgtttttcc aaaggaaacc ggttattccg ttgcccgttt tccatttttc atccttttta
     4501 acgtatcggc ttccgcgtcc ttcaaattga gttacctgcc ttccgccaag caaacgcccg
     4561 cgcccctttc atcatttgaa tatccgtttg accatattta ccagataact atcttcttta
     4621 tataaatcaa tcagcaaagg cacgataccc ggctcataaa ataattctgt cgtatcagat
     4681 gtttctttta acgcggaaat tatctccaac agttcatacc cgtatcgggg ataagcaaac
     4741 aaatagggga ctgcttcccg caaacatgaa aatgcttcgt aactttgcat ttcccatata
     4801 tgttcatctt taaaatcact caaataccat tcatccaatt cactttccgg atcggatatc
     4861 aatcggataa attcatttag ttcgtcctta aacatatcaa atccttaaat ataaaatctt
     4921 ttgattatcc gccgtatcct tattttccac atcatgaatt tcaacgccgt tcatattttg
     4981 cgttttccca agccgtccgc cacagtataa aattattttt tgtatcaatt gcttatatat
     5041 ttaacaactt accgccggac ttgctttggc tgcgtatcaa gattgtccgg ttcgcggcgg
     5101 gcgtttgctt tgtttcaaac agggcggcgc gtaagggatt ttcaaggggc gtcaggcgga
     5161 aaaaggaaat gaaaatgccg tctgaacggc aaaacgtgcg ttcagacggc attttgggcg
     5221 gatcgcgtca tcgcgccaaa gccttcaaac attctttgac caatgccgga cctttgtata
     5281 tcaatccgct gtacacctgg acggcagttg cgcccaagcg gattttctct gccgcatcct
     5341 tgccttccat aatgccgcct acgccgataa tcggcagctt gccgtctatg tggtctgcca
     5401 acagcttcaa cacccgatta cttttttcat gaacgggcag cccgctcaaa ccgccctgct
     5461 cgcctgcgag cggatggctg ccgagacttg atttgtcgat ggtggtattg gtagcgatga
     5521 tgccgtccat ttcgacggat ttgacaacgt gggcgatgtc ttcgatttgt gcttcatcca
     5581 aatcgggggc gattttgacg gcgagcggaa cgtatttccc gtatacagag gcaagctgtg
     5641 cctgtttgtt tttcaaagcc tcaagcaatg cgctcaactc gtcgccacct tgcagcgcgc
     5701 ggaggttttt ggtgttgggc gaggaaatgt tgacggtaat gtagcttgcg tgcgcgtagg
     5761 ctttttcaag gcagattaaa taatcatcgg cagcgttttc gatgggtgta accgcgtttt
     5821 taccgatgtt gatgcccaat acgccactga atttactttt ttcgatgttg cgtatcatgg
     5881 tgtcgatacc gtggttgttg aaacccatgc ggttgatgat gccttgatat tcgggaacgc
     5941 ggaagaggcg cggctgcggg ttgccgggct gcggtttggg cgttaccgtg ccgatttcga
     6001 tgaaaccaaa gccgagcgcg cccaatgcgt cgatgtattc gccgtttttg tcgagtccag
     6061 cggcaagtcc gacagggttg ggcaaatcca tacccatcaa ttttacaggt ttggtacggt
     6121 tgccggttac gggaatcaga cccaatttat aaaccgtgta gagcgcgtcg agcgtgaagt
     6181 ggtgggcttt ttcggcatcg agtgcaaaca ggatgcggcg ggcaagtgga tacatgacag
     6241 ggctttctgc ttcagatgga aatgccgcca ttttaaccga aatcccgcca aacatacggc
     6301 acacaggtca aacttgaacg acaaaacccg ccgcacccaa aaatgtacaa atcggcaaat
     6361 attgatataa tagtcattag atagaaaact ctattaaaag tcggtacaat accaccgatt
     6421 tatttccctt aaacaacctg ccgcaaggca taaaggaacg actgatatgt caaacatcga
     6481 acaacaagtt aaaaaaattg ttgctgaaca actgggcgta aacgaagccg acgtgaaaaa
     6541 cgaatcttct ttccaagacg acttgggcgc ggattctttg gacaccgtgg agctggttat
     6601 ggctttggaa gaagcattcg gctgcgaaat ccccgacgaa gatgccgaaa aaatcaccac
     6661 cgtccaactg gctatcgact acatcaatgc ccacaacggc taaccggtcg tcgcccgaca
     6721 caacagcctc tgctgcaccg cgcaatagag gcttttccct tatatggcaa actgtttgaa
     6781 cccctgccgc aaattacaga cggcatctgc acgacagccg catatgcccc cattccgaca
     6841 accaacagcg aggttatcat gagtcagaga agagtagtca ttacaggctt aggtcaggtt
     6901 tcccctgtcg gcaacactgt cgcagaggct tgggacaccc tgctcgccgg caaaagcggc
     6961 atcggcgcga ttacccgctt tgacgcatcc gacatcaaca gccgtgtcgc cggcgaagtg
     7021 cgcggtttcg acatcggaca atacatcagc gcgaaagaag cgcgccggat ggacgtattc
     7081 atccactacg gcattgccgc cgcattgcag gcaatcgccg attcgggctt ggacgatgtg
     7141 gaaaacctcg acaaagaccg catcggcgtg aacatcggtt ccggcatcgg cggactgccc
     7201 agcatcgagg tcaccggcaa agccgtaatc gaaggcggcg cgcgcaaaat caaccctttc
     7261 tttatccccg gttcgctgat caacctgatt tccggacacg ttaccatcct caaaggctac
     7321 cgcggcccga gctacgggat ggtttccgcc tgtaccaccg gcgcgcacgc catcggcaat
     7381 tccgcccgac tgatcaaata cggcgacgcg gacataatgg ttgccggcgg cgcggaaggc
     7441 gcaatcagca ctttgggcgt gggcggtttt gccgcgatga aagccctctc cacccgcaac
     7501 gacgaccccg ccaccgcttc ccgtccgtgg gacaaaggcc gcgacggctt cgttatcggc
     7561 gaaggcgcgg gcatattggt gttggaagaa ttggaacacg ccaaaaaacg cggcgcgaaa
     7621 atctacgccg aaatcgtcgg cttcggcatg agttccgatg cttaccatat caccgcgccg
     7681 aacgaagaag gccccgccct tgccgttacc cgcgcgctga aagatgccgg catcaatccc
     7741 gaagacgtgg attacgtcaa cgcgcacggc acgtccaccc ccttgggcga tgccaacgaa
     7801 accaaagccc tcaaacgcgc gttcggcgaa cacgcctaca aaaccgtcgt cagctcgacc
     7861 aaatccatga ccggccacct gctcggcgcg gcgggcggcg tggaggccgt gtacagcatt
     7921 ttggcgatac acgacggcaa aatcccgccg accatcaaca tttttgaaca agacgttgaa
     7981 gccggctgcg atttggacta ctgcgccaac gaagcgcgcg acgcggaaat cgacgttgcc
     8041 atttccaact ccttcggctt cggcggcacc aacggcacgc tggtcttcaa acgcttcaaa
     8101 ggctgattcc gcaaagtcgc cgccgacatc gaaatgccgt ctgaaaccgt ttcagacggc
     8161 attttcaaaa caccccgccc gttaccggca cttcgcggca cacaaggcag ccttataata
     8221 cgcaggcaaa cccaagccct gccggattgc gggacgaaac cctgccgtag cagaccgtat
     8281 ttccaatcat atattcaagg tgtccgcctt atgtttcaac acacaggacg acacataaag
     8341 caccgcccta tgtgtcgtcc tgatttggaa ggggttacgc cccctcccaa atagagtctg
     8401 attctaccgc cccgaaggac agatgtccga gtggcggggt tcaaccgaaa aggaaacacg
     8461 atgaaaatcg tttttatcac aacagtcgca tccagcattt acggtttccg cgcccccgtc
     8521 attaaaaaat taatcggcaa aaaccatcag gtgtatgcct ttgtatcgga gttttccgat
     8581 aatgaattgg atattatcag ggaaatgggg gttacacccg ttacctaccg ttcaaaccgc
     8641 agcgggctga acccgttttc cgatataaaa tccaccttcc tcatctttaa agaactcaaa
     8701 aaaatatcgc cggatttggt tttcccttat ttcgcaaaac ccgtgatttt cggcactttt
     8761 gccgcaaaac tggcaggcgt gcccagaatc gtcgggatgc tggaaggttt gggattcgca
     8821 tttaccccgc agccggaagg cataccgtta aaaacaaaaa tcataaaggg gattttgatt
     8881 gccttgtacc acattgccct gccgatgttg gaaagcctga ttgtattaaa ccccgacgac
     8941 aaagacgaac tgacggacaa atacggcatc aaaataaaaa acatccatat tttgggcgga
     9001 atcggtctgg attcgcggca atatccttat tccgaggcgg atattcccga tgaaaaagaa
     9061 cccgtaaaat tcctctttat cggcagattt ctgaaagaaa aggggattga tgattttatt
     9121 cgggcggcgg aacaggttaa ggacaaatac cccgatacgg tttttaccgc tttgggcgca
     9181 atcgacaaat cacgcggggg gggggggggc gatttggaac ggcttgccgc ccgcgatatt
     9241 atccgtttcc ccggttttgt gaacaatgtt tccgaagtga taaaagaaca tcatatattc
     9301 gtattgccgt cttattatag ggaaggcgtt ccccgaagca ctcaggaggc aatggccgtc
     9361 ggcagggcag tgattacgac ggatgtcccc ggatgcaggg aaacggtcgc cgacaaggtc
     9421 aacggcttcc tgatcgaacc ttggaatccc cgcatcttgg ccgaaaaaat gatttatttt
     9481 atcgaaaaca gggaagccgt ccgcctgatg gggaatgcaa gttatgcgat tgccaaagat
     9541 aaattcgatg ccgaaaaagt cgatttgaaa ttgctcgata ttttgaaggc gtaaacaagg
     9601 ctgcccgctt ttgagtttcg gtcatttctg ataaaccccc gtcattcccg cgaaagcggg
     9661 aatccaggtt cgttgagttt cggtcatttc cgataaattc ttgcaacttt gcgtttctag
     9721 attcccactt tcgtgggaat gacggcggag ggttgctgtt ttcccgacaa attcccgcca
     9781 tctaaaatct cgttatccat acaagaaccg aaaatcttgc cattctcaca aaaacagaaa
     9841 ttcaaaacca gaaatcccaa accctcggca ttcccgcgaa aacgggaatc caggttcgtt
     9901 gagtttcggt catttccgat aaattcttgc aactttgcgt ttctagattc ccactttcgt
     9961 gggaatgacg acggagggtt gctgttttcc cgacaaattc ccgccatcta aaatctcgtt
    10021 atccatacaa gaaccgaaaa tcttgccatt ctcacaaaaa cagaaattca aaaacagaaa
    10081 tcccaaaccc tcggcattcc cgcgaaaacg ggaatccagg ttcgttgagt ttcggtcatt
    10141 tccgataaat tcttgcagct ttgcgtttct agattccctc tttcgcggga atgacgggat
    10201 gcaggtttcc gcgcggacgg attcggcatt cccgctttcg cagaaatgcc ggcttttggt
    10261 ttgttgtctt tataagatta ttcggcaatt attctgcggt tttgcgtttg tgtgctgccg
    10321 gaatctcaaa ggattctctg tatttggcaa cggtgcggcg agaaacctcc atgccgcgga
    10381 aagccagcag gttggcgagt gcctcgtcag aatacggcgt gggtgtggac gaggcaaaac
    10441 cagcggcggg aaagggattg agcgaagcaa tgaggtcgag tgcggcttcg agtgtgccgc
    10501 tgtcggtttg gggcaggcgt ttttttattc gcgcgagggt ttggctgcgg ttgccgtcaa
    10561 tgctgtcgag ggcgtttcgg acgatatgca gggcggcggg tttggcagca cattcgcccg
    10621 atctttctat ctgcagtatc agcgattcgt tcaaatcggc ggcggccacg cctgccgggt
    10681 cgaatttttg caatgcagtc agggcttgtt tcagcattgc ttcatccaac atccactcca
    10741 agggcgtatg gtcgaggatg tcttcgatgc tgtcggtcag ataaccctgc tcgtcaagga
    10801 aatcgataag gatgtggacg caggcggctt cttggtcggg aagggggtgt tcgcacactt
    10861 gcgcgtgcag gtattgcttg aaatcttctt cgccgacgat gttggacagc atatcttcgc
    10921 cttcgtctcc gccgatttga cgcgcgggcg cggtgtaatg ggaaacccgg catcggaaaa
    10981 ttcatccgtg tctttgcgtt cgagcagggg attgtccgac agccagtttt cgacctcgcg
    11041 ttcaagttcg atacccgaca tctgcaatac gcgcaaagat tgttgcagcc gctggttgag
    11101 ctgctgggtc tgtttgagct ttattccgag taaggtcatg ataatgtgtt ggaaattgtt
    11161 attttcagcc tgtcggcgca aaaaaatgcc gcaaagcgtc attgcattat aaatggtttt
    11221 aatgagcggg ttcggattct gtctgataac aaaaaacaaa cgaaaatcaa aaaccgattg
    11281 cttataacaa tattaaatcg atttcatagt tttaatagcg aaaatcttgg cgtatagtcg
    11341 catccatagt ttttacaaaa ggaaaacaaa atgtcgattc aagaaattta ttgcaatcaa
    11401 gaaaccggtt acgaatacgc tttccgccaa atcgtactgt aagccgttta cccccatttg
    11461 accaacctga gaaaaggaac aagagcgatg actacctcca aatgccctgt aacccatctg
    11521 accatgaaca acggcgcgcc tgttgccgac aatcaaaaca gcctgaccgc cggtcctcgc
    11581 ggccctctgc tggcgcagga tttgtggctg aatgaaaaac tcgccgactt cgtgcgcgaa
    11641 gtcatccccg aacgccgtat gcacgccaaa ggttcgggcg cgttcggtac gtttaccgta
    11701 acgcacgaca tcaccaaata cacccgcgcc aaaatcttca gcgaagtcgg caaaaaaacc
    11761 gagatgttcg cccgtttcac caccgtggca ggcgaacgcg gcgcagccga tgcagaacgc
    11821 gacatccgcg gttttgcctt gaaattttat accgaagaag gcaactggga tgtggtcggc
    11881 aacaacacgc ccgtgttctt cctgcgcgac ccgcgtaagt tccccgacct gaacaaagcc
    11941 gtcaaacgcg acccgcgcac caatatgcgc tctgccacaa acaactggga cttctggacg
    12001 ctgctgcccg aagcactgca ccaagttacc atcgtgatga gcgaccgcgg catccccgcc
    12061 ggctaccgcc atatgcacgg cttcggttcg catacctaca gcttctggaa cgaagcaggc
    12121 gagcgttttt gggtgaaatt ccatttccgc acccaacaag gcattaaaaa cctgaccaac
    12181 gaagaagccg ccaaaatcat cgccgacgac cgcgaaagcc atcagcgcga cttatacgaa
    12241 gccatcgaac gcggcgagtt tccgaaatgg acgatgtaca tccaagtcat gcctgaagca
    12301 gacgcggaaa aagtacctta tcatccgttt gacttgacca aagtttggcc gaaaaaagac
    12361 tatccgctga ttgaagtggg cgaattcgag ttgaaccgca atcccgaaaa cttcttcgcc
    12421 gatgtggaac aatccgcctt cgcaccgagc aacctcgttc ccggtgtcgg cgccagccca
    12481 gataaaatgt tgcaagcccg tttgttcaat tacgccgacg cgcaacgcta ccgcttgggc
    12541 gtgaacttcc gccaaatccc tgtcaaccgt ccgcgttgcc ctgttcacag caaccagcgc
    12601 gacggacaag gacgcgccga cggcaactac ggcagcctgc cgcactacga acccaacagc
    12661 ttcggccaat ggcagcagca gcccgacttc gccgaaccgc ctttgaaaat caacggcgac
    12721 gcggcacact gggactaccg ccaagacgat gacgactatt tcagccaacc gcgcgccctg
    12781 ttcaacctga tgaacgacgc gcagaaacag gcattgttcg gcaacaccgc cgccgcaatg
    12841 ggcgacgcgc ccgacttcat caaataccgc catatccgca actgctaccg ttgcgatccg
    12901 gcatacggcg aaggcgtggc aaaagccctc ggactgactg tcgaagacgc acaagccgcc
    12961 cgcgcgaccg accccgcact cggccagccc ggtttgctgt aagggggcat tatgtggatg
    13021 gaaattgaag aaatcctgtc ccgcgccgtc cgctaccgga aataaccggg cataaaaatg
    13081 ccgtctgaaa cgtcgtttga ccgcttcaga cggcatttcc acaccccacc ccgcattttt
    13141 atatggtcgc gcatcgcagt gggacgtagg gtgttcgtag ggtgggcttc agcccaccaa
    13201 tcacaccaat cacaccaatc acaccaatcc gccacctcga caaatccgcc acctcgacaa
    13261 atccgacaaa tccgacaatc ttaccggtgc cggcaaacct atgccgtctg aagttggcgg
    13321 aggcggtggg ctgaagccca ccctacagcc cgccattccc gccccacagc caacttttcc
    13381 gcgtcattcc cgcgaaagcg ggaatctaga acgtaaaatc taaagaaacc gttttttccg
    13441 ataagtttcc gtaccgacaa ctctagattc ccgcctgcgc gggaatgacg aaatttttgt
    13501 ttttgatttt ttgtttttgg ggaatgacgg gatttgagat tgcgggcatt tatcgggtaa
    13561 aacggaaatt aagcgttgcg aaaatttatc cgaaatcaca gtaacttttc cgcgtcattc
    13621 ccgcgaaagc gggaatccag gttcgttgag tttcagccat ttctaataaa ttcttgcaac
    13681 tttgagtttc tggattcccg cctgcgcggg aatgacgaaa tttcagtttt tgattttttg
    13741 tttttgcggg aatgacggga tttgagattg cgggcattta tcgggaaaac agcaacctct
    13801 cctccgtcat tcccacgaaa gtgggaatcc aggttcgttg agtttcagtc atttctgata
    13861 aattcttgca gctttgagtt tctagattcc cactttcgtg ggaatgacgg gatacgggtt
    13921 ttcgtgcgga cagatccgtc attcccgcgc aggcgggaat ccggacccgt ccgcacggaa
    13981 acttatattt cgtcattccg ccaatcccac cccaccccca aaaaaaccaa aaaaaaccca
    14041 atctgacaaa atctgacaaa aaacgtcagc ccgaaactga caaaaattgt cacaattgac
    14101 aaaaaaacgt cagcccgccg caggccgcgc cgcgttgata taaaaaaata ccaataccaa
    14161 acagacaaat aaaaaaatat tctgtaaaat gccgagctga accccgtttt ggcacgccgc
    14221 ttgctggaaa gaaggcgaac cgaaacaaaa aagaccacgg tcaaatacat tacgggttta
    14281 cgtttgcggc agatgccgca aatttccaat caaacttaaa cattttcaaa caggagtcat
    14341 ccaaatgaaa gcaatccaaa aaggtttcac cctgatcgag ctgatgatcg tcatcgccat
    14401 cgtcggtatc ttggcagccg tcgccctgcc cgcataccaa gactacaccg cgcgcgccca
    14461 aatgtccgaa gccctgactt tggccgaagg tcaaaaaacc gcagtggtcg agtattattc
    14521 cgacaaaggc acattcccga aaaacaatgc ttccgcaggt attgccagtg cttctgagat
    14581 tacaggtaag tatgtggcta aggtagaagt ttcgggtgct accattactt ctacaatgaa
    14641 aagtcagggt gtaaacaaag acatcagcgg cgcaaccttg gtactggaag gcaaacaaaa
    14701 ctcaggctcg ttcgagtgga cttgtaaagc aggttctgta aaagcaaaat tcttgccatc
    14761 tatctgccgc taataaggac aatggtcggg tttgaccccg ccgtgaaaac aaatgccgcc
    14821 cgaaccgctc gggcggcatt ttctattccc ctttgccgct gtagggctgt agggtgaggc
    14881 gtaggtgcgt agggtgggct gtagggtggg cttcagccca ccattccacc aacccaacca
    14941 actccaccac atcaaggatt tttggcgaac cgttgggatt cggcgggttt cgagtttcgg
    15001 cggggattgg cggaatggtt ggtttgggtt gggtgggaat ggttggattg gcggaatggt
    15061 gggctgaagc ccaccctacg aaacacccta cgaaacaccc tacagcctac acatcagccc
    15121 tacgccctcc ctacccattt ccctgcaaat cccctaaaaa tcctcccgca cgccctgaaa
    15181 acaaggaata atacaggttt ccgcccatcg ggctaaaaag gggataaata tgtcaaatta
    15241 ccggagggac ttcacgcgcg gggcgcacta ttttttcaca ttggctttgc aagacaggac
    15301 gaaggattac ctgatacggc atatcgacag gctgcgcctc gcctatcgtg aaacgaaaac
    15361 acgttatccg tttgaaacgg cggcaatttg tattttgccg gatcacctgc atttcattat
    15421 gcggctgccg gaaggcgacc acgattaccc gatacggatt gcatatttga aaacccggtt
    15481 ttcccaactc atccccaaag agtgccgcgc ccccaacggg agtaaaacaa aaaaacgcga
    15541 agccggcatt tggcagcgca ggttttggga acacctgatc cgggacgaac aagatttaaa
    15601 caaccaccgg gattatattt atttcaaccc cgtgaaacac gggtatgcgg cgagtgtgaa
    15661 ggaatggcgt tattcttcct tcttcaaaga cgttgaaaaa ggcatttacc ctttggattg
    15721 gggagggtaa agctcccgcc gccgccaaaa tccgcaggac ggacgcgggg cagatgagtc
    15781 gggcgggacg tagggtgagg cgtaggtgcg tagggtgggc tgtagggtgg atgtgtaggg
    15841 tgggcttcag cccaccaatc caaccacctc cgccaatcca accattccca ccaacccaaa
    15901 ccaaccattc cgccaatccc cgccaaaaac cgaaaccgcc gaatcccgac ggtttgccaa
    15961 aaacccttga tgcggtggaa ttggcgggat tggatgagtt ggtttggcag aattggtggg
    16021 ctgaagccca ccctacagcc caccctacaa cccgccctaa gtcccaccta cgttccatcc
    16081 gacgcatccg ccctacggtt ccggattccc ctgcgaaccg ccccgcgcac gaaaactgct
    16141 ttacgcggcg tttttcgcgt tttgctgttt tgctataatc cgcccttttt aaggacgggt
    16201 gcggtatgag tctttacgct ttgctcttga ttgcgctggg gatgtcgatg gatgcgtttg
    16261 ccgtcgcgct ggcaaagggt gcggcggtca ggatgcctcc gcgcaaaatt gcagcaacag
    16321 ccttggtgtt cggcacggtt gaagcgctca cgccgctggc aggctgggta ggcggttttt
    16381 atgccaagcc gtttatcagc gaatgggacc attgggcggc tttcgtcctg ctgggcgggc
    16441 tgggtctgaa aatgatgcac gaagggttgt ccggcgaggc ggaggacgcg cgggaaaaca
    16501 aacgggaaag ctggtggttg acggttctga ctgcttttgg aaccagtatt gattccatga
    16561 tagtcggggt gggcttggcg tttatggagg taaacatcgc ctttgccgcc gcaatcatcg
    16621 gtatggcgac gacggtgctg gtggcggtcg ggctgacggt gggaagggct ttgggcgtat
    16681 tgttcggcag gcgtgcggaa tttgccggag gtttggtgtt gattgccatc ggtacatgga
    16741 cgctcttgtc gcacttgggg ctgatcgggt aaggactgcc aaaaaatgct gtctgaagcg
    16801 ttgtttgacc gtttcagacg gcatttttta ttttttgggc gtagggcgaa tccgtaaggt
    16861 gggatgtaag gtgggatgta gggtgggctt cagcccaccc tacgcccact gtttttgatt
    16921 ttttgttttt gcgggaatga cgggattttg gcttactgtt tttgtgaaaa tggcgaaacc
    16981 gcgcaaatgc tgatgccgtc tgaaacactg tttgattgtt tcagacggca ttttttgttc
    17041 acatcgatca agccgcgttg tcgaaaccgc tgtggcgcaa gagtgcgtct atgctcggtt
    17101 cgcggccgcg gaaggctttg aaggattctg ccgcgctgcg cgatccgccg acggcgagga
    17161 tttcctgcca aaagcgtttg cctgtggcgg cgacatcgtc gctttcttca aaggcggcgt
    17221 atgcgtccgc gctcaatact tccgcccacg cgtagctgta atagcctgcg gaatagccgc
    17281 ctgcgaagat gtggccgaag ctcaaggcga agcggttgta ttcgggcggc tggatgacgg
    17341 cgactttttt gcgcacgctg tctaaaacct gttgccagtt tttcagacgg ccttcgtcgt
    17401 cttcgctgta aatcatcata tcaaagaggg cgaactccat ttgccggacg aggaacatgc
    17461 cgcgttggaa gtttttggcg gcgagcattt tgtcgaagag ttctttcggc aggggaacgc
    17521 cggtttcttc gtgggctgac atttgtgcca agacattgta ttcccaaacg aaattttcca
    17581 taaactggct gggcagttcg accgcgtccc attctacgcc gttgatgccg gatacgccca
    17641 gttcgtccac ttgggtaagc aggtggtgca gcccgtgtcc ggtttcgtgg aagaggatga
    17701 ggatttcgtc gtggctcagg cgggcttccc tgccgccgac gggtggggcg aagttgcaga
    17761 cgaggtaggc ggtgggcagt tgcagcgtgc cgtctgaaaa acggcggcgg cctttgtagt
    17821 cgttcatcca cgcgccgccg cgcttgcctt cgcgggcgta gaggtccata tagacgccgc
    17881 ctatggtttt gccgttttgt tgcaattcaa aatagcgcac gtctttgtgc cagacgggga
    17941 cggttttttc ggtaaatccg atgccgtaga gttttttgat ttgggcgaac agtccgttta
    18001 atactttgcc gacggggaag tattttttga cttcggtttc gctgaacgcg tatttggctt
    18061 cgcgcagttt ttcgctggcg tagcccaagt cccacggttg caaatcggcg aggccgaggc
    18121 tttcgcgggc gaaggctttg acttcggcga ggtctttttc ggcgtagggt ttggcgcggc
    18181 gggcgaggtc gtgcaggaag tttaaaactt gttcgggggt gtccgccatt ttggttgcca
    18241 gcgacaattc ggcgtagttt ttgaagccga gcagtttggc ggtttgcagg gcgtttgcga
    18301 gcgtgcggtc gatgttggcg gtgttgtcga atttgccgtc gtctgacagt tcgctggcgc
    18361 gggtaacgta ggcgcggtag atttgttcgc gcagttcgcg gttgtcggcg tattggatga
    18421 cggcgaggta gtgtggaatc tgcaagccga ttttgtagcc tgttttgctt tcgctttgcg
    18481 cggcggcggc aaacatggcg agcgcgtctt cgggaatgcc ggcaagcggt gcggcatcgt
    18541 caaagtaaat gccgaacgcg tcggtcgcgt ctaggacgtt ttgggagaat ttggcggaaa
    18601 gttgcgcgcc ttcggtttgc agttttgcca gttctgcctg ctgttcgggc ggcagttccg
    18661 cgccgctgag gacgaaatcg cgcagatcgt ggttgagttt ggttttttgt gcgggggaga
    18721 gggtgtcgaa ttcgggggaa tttttgatgg ttttgaagcg gttgtacagc tcgatgtctt
    18781 gtccgatttc ggtgaagaag acggtgattt cgggcatcag ttcgttatag acggcgcgca
    18841 gttcgggcgt gtcggcgacg gagttgaggt gcgacaccac gccccaaatc ctgccgacgc
    18901 gttcggtgat gccggtcagg ggttcgacag tgtttgccca gccggtgtgc gtttgggctt
    18961 tgatggcggc gatttgttcg cgcgcttcgg cgatggcggt ttgcagggcg ggtttgatgt
    19021 cttcggtttt gatttgatca aaacggggtt cttcgcccaa atggagcagt gcgttgtcag
    19081 tcataagatg ggtttccttt gcgtgttgtg cttcagacag catatcggca atatgccgtc
    19141 tgaagccgga aatggggtcg gtcggggaaa gttaaagtgt ttcggagtcg gcgggatgat
    19201 ttaacaggcg gtattgtacg cgcaaaaatt cgtccataac cgcctgttgg atttccagcc
    19261 gctttgaaac gggggaggcg aagcggacga tgatgcggta tgccttgtcg tcatacggta
    19321 cgcgggtaac gcgcggtctg gcggcgggcg tgataaacag tttttccgcc tgcacgtttt
    19381 ccaaatgccg ctggatggcg gggatgtagg gcgcgcacaa gggctcgagt acggctttca
    19441 gacggcatac ggcctcatcc gaatccaaat ggatggggac ggggatttcg accgtatgga
    19501 tgacgtagtc gcccaaaata ttgtcgcggc gcacggggtg gcttaacaac aggctgttgg
    19561 ggaaagaaac ggtggttccc gcaagctgtc cgaccaaggg gttcgggccg acctgcatca
    19621 tcagcgtgtt caacaggttg atgtcgacca cgcgcccgcg caggccgttg atttcgatat
    19681 agtcgccgac cgagtattgc tgggtggcag accttaaaat actgcccgac agacacataa
    19741 tcagttcctt cgtcgccacg acgaccgccg ccgccaccgc aaacatcgac aaagccagcg
    19801 tttgaatttg cgccgaccag ataaatgcca gcgaaaacag caccaaaagc agcgttatat
    19861 tgcggctggc aaccaaaaac cgccgcttgc tttcgatgcc gaaatccgga tgccgtttga
    19921 agtggatatt caacagaagg gcgcgcgcca gcagcaaagc cgcaaccgcc gccacggatt
    19981 cgaccgcctc cgcacgtatc gggacggcac cgagccaagt gtccaacata ttccatattt
    20041 ccatttccgc gcccctgtcc gaaaagtata gggtgtagat tttagtggca aaaaaacgct
    20101 tttgccactt tacgggacaa tccccgacct gatgccggag gcaaacgtac cgtttcatct
    20161 tttcccggcg gcacactgat gcggataacg ctgaattagg cggcaaaatc ggctataata
    20221 cacaattata tatgtcgtcc cgcccgcttt ttctacaagg tgcggacttg ttttggcggc
    20281 atcgcaaaat cttttcaaaa tccggcaaaa aatatgactg aacaaaaaca cgaagaatac
    20341 ggcgccgaca gcattcaggt gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg
    20401 tacatcggcg acacgcagga cggcagcggt ctgcaccaca tggtgttcga agtattggac
    20461 aacgccattg acgaagcact cgccggacat tgcgacaaaa tcacggtaac gatacacgcc
    20521 gaccattccg tcagcgtcgc cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa
    20581 gaaggacgct ccgccgccga agtcatcatg accgtattgc acgcgggcgg taaattcgac
    20641 aacaacagct acaaaatctc cggcggcctg cacggcgtgg gcgtgtccgt cgtcaacgcg
    20701 ctgtccgact gggtaacgct gaccatctac cgcgacggca aagaacactt cgtccgcttc
    20761 gtgcgcggcg aaaccgaaga gccgctgaac gttgtcggcg attccgataa aaaaggcacg
    20821 accgtgcgct tcctcgccag tacggaaacc ttcggcaacg tcgaatacag cttcgacatc
    20881 cttgccaaac gcatccgcga actttcgttc ctgaacaacg gcgtggacat cgaattgacc
    20941 gacgagcgcg acggcaaaca cgaaagcttc gccctttccg gcggcgtggc tggattcgtg
    21001 caatacatga accgcaaaaa aacgcccttg cacgaaaaaa tcttctacgc gttcggcgag
    21061 aaagacggca tgagcgtcga atgcgcaatg caatggaacg acagctatca ggaaagcgtg
    21121 cagtgcttca ccaacaacat ccctcagcgc gacggcggta cgcacctgac cgcgttgcgc
    21181 caagtgatga cgcgcaccat caacagctac atcgaagcca acgaagtcgc caaaaaagcc
    21241 aaagtggaaa ccgccggcga cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa
    21301 ctgcccgacc ccaaattttc gtcccaaacc aaagacaaac tggtttccgg cgaaatcggt
    21361 cccgtcgtca acgaagtcat cagccaagcc ctgaccgact tcctcgaaga aaaccctgcc
    21421 gaagccaaaa tcatcaccgg caaaatcgtc gatgccgccc gcgcgcgcga agccgcccgc
    21481 aaagcccgcg aaatcacccg ccgcaaaggc gtaatggacg gcttggggct acccggcaaa
    21541 ctcgccgact gccaagaaaa agaccccgca ttgtcagaac tttatttggt cgagggcgat
    21601 tctgcaggcg gttccgccat gcaggggcgc gaccgcaaat tccaagcgat tttgccgctt
    21661 aagggcaaga ttttgaacgt ggaaaaggcg cgctttgaaa aaatgctcgc cagtcaggaa
    21721 gtcgccgctt tgattactgc gttgggtgca ggtatcggca aggaagaatt caatgccgag
    21781 aagctgcgtt accaccgcat catcattatg accgatgccg atgtggacgg cgcgcacatc
    21841 cgcaccctgc ttttaacctt cttctaccgc caaatgcccg agctggtcga acgcggctac
    21901 atctacattg cccagccgcc tttatacaaa gccaaatacg gcaaacaaga acgatatctc
    21961 aaagacgaat tggaaaaaga ccaatggctg ctcggtttag ccttggaaaa agccaaaatc
    22021 gtttcagaca gccgcaccat cgaaggcgaa gagcttgcca ataccgccaa acaattcctg
    22081 ttggccaaaa ccgtcatcga acaggaaagc cgcttcgtag acgaactcgt cctgcgcgcc
    22141 atgctacacg cgtcgcccat tgatttgacg tcgtctgaaa acgccgataa agccgttgcc
    22201 gaactttccg gtctgcttga cgaaaaagaa gtcgccctcg aacgcatcga aggtcatgaa
    22261 ggacaccggt tcatcaaaat cacgcgcaag ctgcacggca atgtggtggt gagctacctc
    22321 gagcctaagt tcctcaacag caaagcctat caaaccctta cccaaaccgc cgccgcactt
    22381 aaaggcatgg tcggcaaagg ggcgaagctc tacaaaggcg acaacgaata cgacatcgac
    22441 agctttgaag gcgctttgga catcttgatg agcgttgccc aaaaaggtat gtccatccaa
    22501 cgatacaaag gcttgggcga gatgaacccc gaacagctgt gggaaaccac gatggatccc
    22561 gccgtgcgcc gcctgttgaa agtgcgcatc gaagatgcca ttgccgccga cgaagtattc
    22621 gttaccctga tgggcgacga ggtcgaaccg cgccgcgcct ttatcgaaaa caacgcgctg
    22681 attgcccaaa atatcgacgc ataagtgccg ttttaaaaaa ggagacgggc atcgtgccgc
    22741 gtctcctttt ggtttgtcaa acggaagcca tgccgtctga aaccgccttt cggagcaaaa
    22801 ccatggtcag catttttgat attttcaaaa tcggtatcgg gccttccagt tcgcatacgg
    22861 tcggcccgat gaaggcagcc gccgcctttg cggcaggttt ggatgcacag gctgttcgca
    22921 tcgtcatcga catttacggc tcgctcgccc tgaccggata cggacacggt acatttgacg
    22981 cgctgatgct cggtttggaa ggcagcctgc cgcacgacat cccgcttgtc ggcattcccg
    23041 aacgcctcga acgcatccgc acgcagcaca tcctccggct caacgggcaa gaaatccgct
    23101 tcatccccga ccgcgacctg aacatactcg gcaatcaagt gctgcccaaa caccccaacg
    23161 gcctgcgttt taccgcctat gcttcagacg gcacggtatt gaatgaacag gtttattatt
    23221 cggtcggcgg cggctttgtc gttaccgaag aagattttga ccggcaggcg gaaacggaaa
    23281 aagccgttcc ctatccctat accagttgcg ccgaactgct tgcccgatgc cgtctgaacc
    23341 ggctcgacat ctccgaagtc gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag
    23401 ccgaaatccg ccgccgcgcc gctgccgttg ccgaggttat ggaaggctgc atcaaacgcg
    23461 gcttgggtgc ggacggcgaa ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc
    23521 ttgccgccaa gctcaaagtc ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga
    23581 tggtgtacgc catggcggtc aacgaagaaa acgccgccgg cggacgcgtc gttaccgccc
    23641 cgaccaacgg ggcggcaggc atcattcccg ccgtattgca ctatttccgc aagttcaacc
    23701 cccacgccac acaggaacgc gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc
    23761 tctacaagac caacgcctcc atttccggtg cggatgtcgg ctgtcagggc gaagtcggcg
    23821 tagcgtgttc gatggcggcg ggcgcatacg ccgaagtcat cggcggcacg cccaaacaag
    23881 tggaaaacgc cgccgaaatg gcgatggaac accatttggg gctgacttgc gaccccgtcg
    23941 gcggactggt gcaaatcccc tgcatcgagc gcaacggcat cgccgccgaa aaagccctca
    24001 aactcggcac gctcgcgctt ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag
    24061 tcatccgaac catgctgcaa accggccggg atatgaaggc gacctacaaa gaaacctcgc
    24121 ttgccggact cgccgccacg ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg
    24181 agtgctgagg atggacagaa acgaaaatgc cgtctgaaaa cagtttttta gacggcattt
    24241 catttatttc ttgcggcttt accgtttcag tgccagcgtc aacacgcccg ccgtaaccag
    24301 ccccaagcct atccattcct gcgtgttcgg gcgttcgtct aagaaaacca ccgccatcag
    24361 gaatgcaaac cggcgttgtt tgcagcatcc ggcaagaatt tttaaaaggt cttttctaaa
    24421 atatgcagat attcgaatgt cgaatttgcc ttatggttac ggttttctgt tttatctgcc
    24481 ttaaaccgcc ggtattccgt ttgaaccaaa tcatatttgc cgaaacgctc aaaaatctcc
    24541 cttacctgcc cgacagacat caagccttcg ttgttgtagc tgagaaaaat atattgaaag
    24601 tccgcatccc taatcaaagc ctcaaatacc ggaaccacat ccgattttga gcaagtccgc
    24661 gctaaaacac atcgtacagc gcaaaaccgg atgggaatgg caaaatccgg aaacaaacat
    24721 ttttcaaagc atacaaacaa aaaaatacca acctccggtt ggtattcctt aac
//
LOCUS       DAWFIT010000023        22852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFIT010000023 DAWFIT010000000
VERSION     DAWFIT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.023"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(652..2625)
                     /locus_tag="WGQ85_000872"
     CDS             complement(652..2625)
                     /locus_tag="WGQ85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0750330.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAATQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNRNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSIVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQGLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRKSGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2636..3197)
                     /locus_tag="WGQ85_000873"
                     /pseudo
     CDS             complement(2636..3197)
                     /locus_tag="WGQ85_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
     gene            complement(3194..3712)
                     /locus_tag="WGQ85_000874"
     CDS             complement(3194..3712)
                     /locus_tag="WGQ85_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0750331.1"
                     /translation="MPAIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLGTVDIA
                     ITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGIDLDDAK
                     RRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVNRIEWF
                     KAGAINVTEMPS"
     gene            complement(3663..3896)
                     /locus_tag="WGQ85_000875"
     CDS             complement(3663..3896)
                     /locus_tag="WGQ85_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ0750332.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            complement(3893..5038)
                     /locus_tag="WGQ85_000876"
     CDS             complement(3893..5038)
                     /locus_tag="WGQ85_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0750333.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(5028..6359)
                     /locus_tag="WGQ85_000877"
     CDS             complement(5028..6359)
                     /locus_tag="WGQ85_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0750334.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(6359..8530)
                     /locus_tag="WGQ85_000878"
     CDS             complement(6359..8530)
                     /locus_tag="WGQ85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ0750335.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            8685..9267
                     /locus_tag="WGQ85_000879"
                     /pseudo
     CDS             8685..9267
                     /locus_tag="WGQ85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9311..9580)
                     /locus_tag="WGQ85_000880"
     CDS             complement(9311..9580)
                     /locus_tag="WGQ85_000880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750336.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(9520..9885)
                     /locus_tag="WGQ85_000881"
     CDS             complement(9520..9885)
                     /locus_tag="WGQ85_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750337.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(9889..10266)
                     /locus_tag="WGQ85_000882"
     CDS             complement(9889..10266)
                     /locus_tag="WGQ85_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0750338.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(10331..11740)
                     /locus_tag="WGQ85_000883"
     CDS             complement(10331..11740)
                     /locus_tag="WGQ85_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ0750339.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(11743..11940)
                     /locus_tag="WGQ85_000884"
     CDS             complement(11743..11940)
                     /locus_tag="WGQ85_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0750340.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(11937..12587)
                     /locus_tag="WGQ85_000885"
     CDS             complement(11937..12587)
                     /locus_tag="WGQ85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0750341.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(12577..12999)
                     /locus_tag="WGQ85_000886"
     CDS             complement(12577..12999)
                     /locus_tag="WGQ85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0750342.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(12999..13397)
                     /locus_tag="WGQ85_000887"
     CDS             complement(12999..13397)
                     /locus_tag="WGQ85_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750343.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(13462..14370)
                     /locus_tag="WGQ85_000888"
     CDS             complement(13462..14370)
                     /locus_tag="WGQ85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0750344.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(14370..15380)
                     /locus_tag="WGQ85_000889"
     CDS             complement(14370..15380)
                     /locus_tag="WGQ85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750345.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTSGKLLPAQKEWAEGVLKQPGGL
                     AFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESE
                     GK"
     gene            complement(15611..16027)
                     /locus_tag="WGQ85_000890"
     CDS             complement(15611..16027)
                     /locus_tag="WGQ85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0750346.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(16155..17480)
                     /locus_tag="WGQ85_000891"
     CDS             complement(16155..17480)
                     /locus_tag="WGQ85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0750347.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(17467..19035)
                     /locus_tag="WGQ85_000892"
     CDS             complement(17467..19035)
                     /locus_tag="WGQ85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0750348.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSLQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTIASQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(19035..20657)
                     /gene="terL"
                     /locus_tag="WGQ85_000893"
     CDS             complement(19035..20657)
                     /gene="terL"
                     /locus_tag="WGQ85_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0750349.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVETQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            complement(20678..21184)
                     /locus_tag="WGQ85_000894"
     CDS             complement(20678..21184)
                     /locus_tag="WGQ85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0750350.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            complement(21186..21383)
                     /locus_tag="WGQ85_000895"
     CDS             complement(21186..21383)
                     /locus_tag="WGQ85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750351.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            complement(21380..21646)
                     /locus_tag="WGQ85_000896"
     CDS             complement(21380..21646)
                     /locus_tag="WGQ85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750352.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            complement(21658..21986)
                     /locus_tag="WGQ85_000897"
                     /pseudo
     CDS             complement(21658..21986)
                     /locus_tag="WGQ85_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21983..22300)
                     /locus_tag="WGQ85_000898"
     CDS             complement(21983..22300)
                     /locus_tag="WGQ85_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750353.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWVNSQAAVDGSE
                     GAR"
     gene            complement(22197..22616)
                     /locus_tag="WGQ85_000899"
     CDS             complement(22197..22616)
                     /locus_tag="WGQ85_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750354.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYNRALGYGN"
     gene            complement(22588..22824)
                     /locus_tag="WGQ85_000900"
     CDS             complement(22588..22824)
                     /locus_tag="WGQ85_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750355.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
BASE COUNT         4576 a         6516 c         5983 g         5777 t
ORIGIN      
        1 ctgccgagtt tggggtctgg attcccgcct gcgcgggaat gacggcggcg ggggtttctg
       61 ttttttccga tagattcctg ccgagtttgg ggtctggatt cccgcctgcg cgggaatgac
      121 ggcggcgggg gtttctgttt tttccgatag attcctgccg cgtttggggt ctggattccc
      181 gctttcgcgg gaatgacggt ggcggggggt cggttttttc cgatagattc ctgccgcgtt
      241 tggggtctag attcccgctt tcgcgggaat gacggtggcg ggggtttctg ttttttccga
      301 tagattcctg ccgcgtttag gggctggatt cccgctttcg cgggaatgac gaaagcggcg
      361 ggaatgacga agagtggcag gattggcggg gcataggttt cgggggattg gcggcgcggc
      421 cggtttcggt cggattgctt ggatcggcgg gctgaagccc atctctacag cccatctcta
      481 cagcccatct ctacagccca tctctacagc ccgtctctac agccatctct acagcccgtc
      541 tctacagccc gtctctacag ccatctctac agcccgtctc tacagccatc tctacagccc
      601 gtctctacag ccatctctac agcccgtctc tacagccatc tctacagccc cctagtcttg
      661 cagtgcctcg agcaagccgt ctcggcggcg gcggtttcgg ggtccggttg atgttcttcc
      721 actctttctt tgcggttctt cgttggtttc aggtatttcg gggcctatcg gggagctggc
      781 ggcgtttccc gaggcggctt tgccgatggc catccagtcg caactgcctt cgtaccagtt
      841 ggtaataatg gcggcggcgt ggttttttgc gcggataaac agccagttgt gttgccttcg
      901 atcgccggta acgcgtcccg aatggcgttc ggatacgaag catttgacgt tgccgtcggc
      961 aaaggcgacg gggaagacga cttcgttttc gatggggccg ccgcttcgtg tgaacctgta
     1021 gctgccggtc tgtatcattg tgccgtcggg gaatctgaca atatccacgc cgccgatggt
     1081 ttggcgtgtg aattgcgcgg caacggcggt gctgattttt tcgtccagcc cttggatttg
     1141 ggcggcggtg tgggtgtggg cgcggtcggc tttgtcttgc aaaccttgcg ccagcgtgcc
     1201 cgcgtccagc gcgccggcgt tgacggtttc gccgtgggac tttatccaaa acacggcttc
     1261 gcccaaggtg tcggcggctt tgatgcacag ttttaaaacc agggctttgg ggcggttttc
     1321 gtcgccgccc gttgccattt tgctgtccgg gcgcggggtt aaaaagccgt tgtcgcttaa
     1381 attttcgtcc gtccaagtcg atacgatcga gcttctctgc ctttcgttgt ggtcttcgta
     1441 accgagggct gccgcgtgcg ggtggtttac ccagtgtgaa aatactttgt ggacgtgccg
     1501 tttgatttcg tcttcctgct tcgttccgac tgccaagccg ttggcggcgt tgcggataaa
     1561 gcggtcttcc gcctgcggga cgttttggat gctgccgtat tttccggtca gcaggcggta
     1621 cagctcggga taagcggttt cggttacgcg cgtgcggatg tcgtcgaacg ccagccagcc
     1681 ggtcgggatt tggtcggacg gaaaccacgc ggtgatgccg atgtcggtac ggctcaaatc
     1741 gggcaggcgg ttgcggttgc ccaaggcgcg gtagaggtcg gggaaggtgt tttgcgcaaa
     1801 ggtcgtgccg ttggctttga gatagcctgc ggggtttcgg acggctttgg gaaaggatac
     1861 gatcgcgccg acggggatgc cgtccgaatt gagttttttc catgcggacc agttgtcgtt
     1921 tgcgctgctc gtttggtggc gttcgtacac gtcggacgtg taggcgggat aacccaactg
     1981 cctgcaccag ccgtgtttcg tgccggcgat gacttggata tggcaggcag tgttttcaac
     2041 gggcaggttg gagctgccgc ccgctgtcgg cagggaatag atgccgtctg ttttgagggt
     2101 gttcaaatcg ccccggaatg tttcgacttt gaaattgccg atgccgtatc cggacagtgt
     2161 gttcggtttg ccttcgatgt cttcggcaaa acgcggtttg ttggctttgt ggaagatgtc
     2221 gtggttgttg taggaaatgc ggttgtttgc gctgtgcagg cggagggtgt cgtcgccgaa
     2281 gacgatgtgg gcgttgtcgg catccgcgcc gatgtaggct ttgtggccgc cgttccagtt
     2341 cgtctggttg gcggataaat ggatgccgct ttggaattgg gttgcggcgg taaaggtttt
     2401 ttggccgccg atttcttggt tgccggtggt aactacagcc agtctattca gtgctgccag
     2461 taattgatta ggctgcgact tatccggctg gataccggcc gcagtcagca cggccagcag
     2521 ctcggattgc acttggttga gccaccacgc gggcaggatg gtgcccaatt cggacacgcc
     2581 gtcgccgtcg tggaaggtct tatcgggggt ttcgatgggg tgcatggttt agtccttatc
     2641 ggtaggtaaa tcggatagcg gtgtgggcgg gcttgaggcg gttgaacagg ctttcgatca
     2701 cggcatcgct gtaatcggtc agcctgtcgc ccgccgccga gataccggcg cggaatcggg
     2761 taatgcggtt gttgccgccg cgcacgttaa cgtgccacac ccacatgatt tcctgcggcg
     2821 caagacggtc gcccgcacgg ttgacaccgg cgcggaacgg ctgcggttcg tcgatttgga
     2881 tttgatagcc cgccgcctcg gccaaacgca caaaataagg aatactcaag ccgcctgttt
     2941 cgtttagctt ggccatgacg gccaacacac ggcgctggcg gtttttgccc gtaccgtcca
     3001 aacctaatac gcgctcccaa tcggccagca tttggccggc gctgcgcggg tcgacggcat
     3061 cggcaacgct ttgagccgat tccgctaccg catccagcgc tgcgccgtct atttctgcct
     3121 gcgcccgcac acgcggggca ttgcgggcat acgacacggg ggggcaacag accccgcaag
     3181 atgtcttgat agctcatgac ggcatctccg ttacattaat cgcgcccgct ttaaaccact
     3241 cgatgcggtt aaccgtatcg gcggcacggt tggccgtcgg cgcagtcaga cggcggtcga
     3301 tcacaccatc cacattgctg atagccgcct cgatttgcga cacagtcagg ccgtcgccgg
     3361 ggatcagggt gtcgaaatat tcttttaggg ccgtccgtat gcggcgcttg gcatcgtcca
     3421 agtcgatgcc gtcgagcttg acttgcacgg taacaggcac cgccgttacg gttggcttga
     3481 gtaccagcgc attttttgcc gttaccgggc gcatctcgtc gatataagcc tgtacgcggc
     3541 gcacagtttc ttccgacggc acaccgtcgg cggaggtaat ggcaatatcc accgtaccca
     3601 agccgcggcg cagcggataa acatatgcgc tggttacgcc gtcaacactc aacgcccagt
     3661 ttttatagtc gtaacggttg ccgcctgcgg gcggtcggcg gatggctggc aaagccgtat
     3721 tcctgcaaat gttcaaggtc ttgcagggtt tcgtcggcca agccgctcaa ctgcacgcgc
     3781 tgtatcggct cggacgacac caccagcgtg atttttcccc gaaaggccgc gcgcagggtt
     3841 tcgccgatat ttttagcagt ttgtgccgtt tttttcgcca atttactcaa actcattcaa
     3901 acaccgccgt ttctgcttgt tttttgccgc cttttttgcc cttatggctc acgcctttgc
     3961 gtttgccttt gcgcttgcgc gccgcctcgg cctttttggg gtaagcgtcg ggtgtccaaa
     4021 taccgtcctc tttgagccgc agctcggttt gcgtgccatc catgcgggat agcataaacc
     4081 gccgccccat cagaaaaaac accgcatcga taccgtgctc gtcgtcgatc acatgcacac
     4141 gctggccagg ttgccacaat acgccgtcgc gggttttatg gccgcccacg gttatcgtga
     4201 gtgtcaatcc ctccagccgc cagtcggcca gctgcttttt agcctgcttt tgcaatgcgg
     4261 ccaaattgtc ggcatcggac accaccaccg ttttagggcg gtgcagcgtc atcgtcgggt
     4321 ctttgtacac ccactttaaa tcgtgtttgg cgctgtcgcc gctgcggccg tgcgattgcg
     4381 ccaaaaaagt aacctcggaa aagcggttgt cggtatccca ctcaatgtcc atgcgctcga
     4441 tattgcggcg gctgtcggtg cggctccaac acaatgtcgc caccggcggg ctgctgtaat
     4501 ccgcaccgcc caccaccaac gtgccgtccg gctccagcca cggatgcagc ccgaccgagt
     4561 tggcaatatg ggttaatgcc tgccatacgg tttcgcccgg ctcgatgtcg attttgtcca
     4621 aagcggggtt gttttcggcc ttaagcacca ccgctttaat ctgcggccac ggcgcggcca
     4681 gctttttggc tgcatccaat accgtcatgc cctttacatt gagctgcggc gcggagcaat
     4741 ccaccaaaaa accggccaaa tcacgcccgc tcaagctcaa ctcgcggccg cccttgcttt
     4801 tgccgtggcg ctggctgccg atgatgcccg tcatcacgat ttgcccgtct atcactacct
     4861 cgcagctctc tccgcttaaa tcgggtatgg ccgcctccgg ccccaacctg ccgatgacaa
     4921 aatcgaagct gtcggcaggg attaaaaagt cgctgtcgat gtcgtagcgc tcccagtggc
     4981 ggtgctcttt accgcccacg cgcaccgaca cggcatagcc gtatgaatta ttttgcatag
     5041 ctgttgacca aagtgccgcg cttgataaac gcggggtggt ggatatgggg attgagccgc
     5101 accagctctg ctgcgcgggc tatatcgccg taaaactcgt gggcgatttg gtgtatcgta
     5161 ccgtcgattg gggcttggcg cacaatcagc ggcggctttt ggttgatgac cgccgcaacc
     5221 aacgcattca gacggcctgc cgccgcgcgc agggattctg ccgtttggta agcctcggta
     5281 tacacggcgt tggccgtcag cccaccagac tcggcagcag ccgtctgcac cgcccgtaag
     5341 gcggcaatct cggcctgcat acggcggcgc atggcgcggt taacctcaat caaatcgggc
     5401 gcggtcatct cttcgccatg cgcctcgatt aatgccgttg ccaccgacaa cagcgatgac
     5461 gtggataaca ggcgcaccgc ctgcgctacc ggctgcacct gtttggcggt taaacggttc
     5521 aggcggtttt gcaggccgtc tgaaaagcgg ccggtcagca aattatcagg gatagcggcg
     5581 gcacggtcgg caacagccgc agccccgtca aaccgctgtc gcggcgacca accggcatgg
     5641 tgcatggcat tatcggccaa acccgcctca cggcgtatgc cagtatctac catgaccgat
     5701 atatccgcaa acagcttggc cgagccgttt ttaaacgccg ctgcgctgta tccgccccga
     5761 tcgggaaagg caattttgtc caaatcaaac aaacggcgca ccgcaccaaa cgtacccgat
     5821 gccgcactcc aaatgcccag cgcgctgccc cataaagccg atacgcccgc atccaccgcc
     5881 aacaccgcat caacaaagcc gatagccgcc tcgcggtagg tgtcgatatc agcaatcaac
     5941 gcctcaagct cgaccaaaaa ggcgttttca aaaacaaaga tttcctgcgc ttcggccgcc
     6001 tcgcggaaag taatatcgat gcccgcataa tccacataat cggcctcatg tcggtaactc
     6061 catgatgccg caatcatgtt gtgcatccgc ccccaaacag ggtgcaccag cacgccgccg
     6121 cccggctgct ccagcgcatc cagcagcttt ttcagacggc ctgcatagcc cttgccccaa
     6181 aacaccgcat taatctgcac ctgccgcccg gtcatgccca tgtcttcaag gtcgataccc
     6241 tgcacaaacg gccgcgcatg ctcggccaat gccttgccgt tgctctcgtc caccacctca
     6301 atatcaaagc cgacgccctt gtaagatgcg tcctgcaata aggtatgcca tccgctcatt
     6361 attgacccgc tccacggccg aacatggcca cttgataccg cgatacctca ttcgcgataa
     6421 cacgtccgtc gatattcagg ctcacattgg tattgatggt ctgacttgcc gacgccatcg
     6481 cactttgcac ttgtgccaaa cctgttgtaa ctgcagccgt atcctgcgcc aatgctgcct
     6541 gatacgccgc cgtctgctgc tgaatggctg cctgatacgc cgccgtctga gttgaaaaca
     6601 acggagacaa catcttatcg gtaggctgcg ctgacggagc agccaaatac tgagggacac
     6661 caccccgcgc ctgttcccgc ttcggcgcag gttcaaatcg gataccgttg tttttcagac
     6721 gattaatcat ttcaggagtt gtcttggtac cacgcaaacc ctttggcaat gtgccgtcac
     6781 ccaaagactc ggaatacagc aacataccta acaaacccgc ccgtttaact aacgctggat
     6841 tattcatgag cccgctaccg gcagattttc cccatgacag caacttaccg cctgccgctg
     6901 tggcacctgc tgccacgccg cctgcggaag ccttacccca taccaacgct ttactaccta
     6961 catctttcag aaagcctgca cctttaccgc caccggcggt taataacatt gcggcagacg
     7021 ccgctgttgc cgccgtcgtc aatgcttgca gggctagagt ggcattaggg tattccgctg
     7081 ccagcttttt aaacgaggtt tccatatcca ccaatgttcc cgtcagactt tcccgcaaca
     7141 ttgccagcga ttcctgttgc tcttgttgcg ccgctgccga caacattcgc gcctcgttgt
     7201 tcttagcaat ttttccgtta tccaacgtta cgccagccaa cgaagccata tattcacgga
     7261 tttgcgtcat atccgttgca gccagcaaac cttgttttgc ctgcaaatca ggcagcagtt
     7321 gcgccaaaag cgcgccctta agcatatttg cctgctccgc cgccgtctta tcgcctgcag
     7381 ccgcgcgttt cttataatct tggtattgct tatcctttac tagcatggca tcggcaagac
     7441 gggacaacac ctgcactgcg ttttcgccgt tttgcttgcc ttgcacaacc gagcctatcc
     7501 aatcgacacc tttcttcggg tcatttggat ttgccatctt cttcagacga cctatcgtgt
     7561 aaggcgacag agttttactc aaaagatttt gcacattagt cgccgcttcg gcaggactgc
     7621 ccgatttatt cgccgcagat tgtaagagtg agagcaggta gtccaaaccg ccgacaccat
     7681 tcatccctgc ctgttgcgcg gcagggagca gactcggcag ctcccgaacc atatcccgca
     7741 cctcgaaagt gccgtctaaa cccgattgca agacgtgctc aagcccgagt tgcaggtctt
     7801 taccgctcat gccgccatct ttcagggttt taatcagttt tgccgtatcc tcgccactgc
     7861 cttctgaggc aagtgcaaaa gcatatgccg cctgcgcttc attcttggtt tgggcaaaat
     7921 tcagactggt ggtcatcatg ccgctgatta aatccaaagc cttatcgtgg gtcccgccat
     7981 ttttctcgac aagttcaagt gccaaatcct tgatctgttg cgcaccttca gttgcaatcc
     8041 acgctgccga tttactgtta tcctcaataa atgcctgtct ggacacgcgg ttgatgttct
     8101 catcaagctg ctttctgttg tccatagcag gcttaagcac cgtatacgct gccgcgccac
     8161 cagccaccgc agctgcaccg aatcttccga tttttcccat cttgtccgca aatcccgtgc
     8221 cctgtttcag ttccgcgttc agctcgcgaa ttcggttacg cgtagcgacc gccgcccgtg
     8281 ccaaatcatt ttgtgatgcc ttgccgctgc gcgccaaacg gttaaacgca gcctgtgtca
     8341 gcatgatttc gcgttgaatc tgtttttctg agcggatgcc ggtcaacgca taagtatggc
     8401 tttgccgttc aagttgcgcc cgcgatttcg cggtacgatc taattgtcgt tgagtatcag
     8461 ccagtagccg tctcgctccg tcatctcggg cggttaacac caacgacagt ttcatgttcc
     8521 cgtttgccat aaaaagcagc ctttaaacgt attaactcaa ggtcaattat cgtttaaagg
     8581 ctgtttaagg gaggcggtat gggcttcaac cccgttttcg tgctttattt gtttgttgcg
     8641 ttataatacc agaatgataa ccattttatt caaagggata ttttatgaat atgaaaaaat
     8701 tgatttccgc aatttgtgtt tcaattgttt tatcagcctg caaccaacaa tcaaaaacgg
     8761 cacaagccga agaacctgtc caaagtatcc aggctgctga ttgtaccgcc ccaatggaca
     8821 tcacagttga acaatatctc atcaatttgg agcaagcatt taaaactcag aacgtctcaa
     8881 caaaaatcca taataaaaat attgtcaaga ccgattgtgg ttatgacctt actttggtaa
     8941 tggattttgg ggcgattgcg ctcaaactgg acgagcagca aaaaattaga gctatctcag
     9001 taggctacat tttaaaaacc gacggagaga aaggacaaaa tctagtcaat aatgccataa
     9061 atggattaca cagtattcag gcagttctgt ctttaactac cacagacaaa ttgggcgaat
     9121 cggaagcagg aaaacaactt cgtcaaagaa tccaatcaga caggagcaac agcgcaaaaa
     9181 gacgttccgg cagatggtat tttatatagc gttgtttttg aaaaagaaac aaacaccatt
     9241 gcaataatcg gcagaaaaca accctaaccg tcaaataaaa aaaaggtcgt ctgaaactca
     9301 gacgaccttt ttattttccc tccggctttc tcagccgttt cgacacaatc actccctctt
     9361 ccttcggcgt ttttatgccc aggctcgaca ggatgtcttc aaaccatgcc gacagctcgg
     9421 cgtggcacat atcgcgcact gcgtccgccc ctatccccgc ctttgccatc aggatgaccg
     9481 cctgacggta gcttttgtgt gccgtcaggt agctgcgttt tactgctccg ccgccgacgg
     9541 cgcgtcgggg ctcgccgtag cggcggacgg ttttaggcac aaatcctcct gagccgccat
     9601 cacggtatgg tagtcttcgg tagagaggtt ttccatcagc cactgcgccg tcaggatgtc
     9661 ggatgggata ccttgggctt caatctgctg cgaccagtag gtcagcgtct ctaggacgtc
     9721 gcgttgcagc agttcggctt ttgtggcgtt ttcgggcagc tcgggcaagt catcaatcag
     9781 ggcgaacgcc gccagttcgc cgccgacggt cagtggcttg aggcgtaatt cagatacgac
     9841 tttgccattg taggtcagcc cgtgggtcag tttgacggta atcacgcatt actccttgat
     9901 tttgtgcaaa gcaatcatct caatgtcgat gcgtgcctcg ttatcgactt catattgctc
     9961 gctggtatcg acggtaaagc agtcgaggta ggaagtgcgg cgaccttcgt cgttgatggg
    10021 gtaaatcgtg attttcgcct tggtgatgtt gtcccagtcg atttccgtac cgtcgatcgg
    10081 aatggcggcg gtaacgctta atttgtgttc ggttacgccg tcacaataac cgttgactcg
    10141 gccgttgcgg ttcatcgttt tgaccggctt gcggcctgta gtggtttgcg gtttgatgct
    10201 cacaatctcg atatcgcggc cgttcacctc cataatcacc gcgccggtat aggtagcgtc
    10261 gctcatgctt tatccttttt taaggtttca gacggccttt aaacctgttt taaacgccgt
    10321 ctgaaaaggg ttacaaaatc aaatcaatgc gcccggcaaa gacgtgcagg ccgttgacca
    10381 catcggcggg gataatggca ttaacacggt tggggtcgtt ttgcgcacgc gccaccacca
    10441 gcttgccttt gttggcctcg gcgttttcga tgatttcggc ttggtcgagc ttaatcagca
    10501 cgtccaaaat ctcgctctta accttgggca gcaggcggtc gctcaatttg tcgcgcggaa
    10561 aacgcagggc aatgcgctct ttaacgctgc ggcgcacata atccagcgtg cgaatggtgg
    10621 taatgtcgag tagtgccggg tcgtcggtgt tgttggccga cttggtatag gttgatacgg
    10681 cacgcataat ctgcacgcgg ttgttgaccg ctgtgagcgg ggtcaagccg ttgtacagcg
    10741 cattgttgca ttctgcaaac cacggccatt gcgcatcagg tgtaacggcc agccctttga
    10801 tttccagcgt gttcagcggc ttggcagggt cttcttcaaa ggccaacacc gccgcataac
    10861 ctgcggcgat gatgccgttt ggctctaccg cacctttgta ccaagcacag gtaatgcggc
    10921 cgtcgttgat tttgccggta gcggtggtgg ccgtgctcaa ggccgcactc atacccaata
    10981 cgccgataca gccgcgctgc tcgatggcgt tgaatacgtt ggtaatatgg ttgctcaagg
    11041 ctttggcgtt ggcgtcatcg ctaaacgggc tgcaaataat gtgataatgc ttgcccgcca
    11101 ctttggacaa ggccgtggca atgtccgcat ttttggcacc gccggtaaag acattggctt
    11161 gataagtcag gccggtattg ccggtgctca cggttaagcc gctctcgttg ccgatctcgc
    11221 ctttgtgttt ggccgtcagc gtaacggctg cgccgctgcc ggatgcggta acggttacat
    11281 cggcggcagt gatggcggtt ttcagacggc ctgccactgt ggcggcggtc tcgccggtgt
    11341 taacggccgt gcttacctgc ttgccgccaa tcgtgatttc caccacgccc ggcgcggtgg
    11401 ccgtgccgga aagggtaacg gttgcggttg cctgcacgcc tgcgctgtgg tcggcaatac
    11461 cgataacggt caaatccaaa taagggttgt tggcaaatgc ttggcgcacc atcaaatgcg
    11521 ccagcgagcc ttgtccgaac aaatcggccg cctcggcatc gctaaatagt tgcaccggct
    11581 ctaaggcggg ctgtatgccc gcggtcagca tgggtgcaac catcaatacc ttttgcggat
    11641 tttgcggcaa accttgcacg gcattgcggg tgttaaattc aatatactgc ccgggcacgc
    11701 ggatgctgcc cggaatcgtg tcaaaatcaa tatggggcat cattactctc ccttagtatt
    11761 gcgggttttg gtatttgggg cggcatcctc aaccaccacc aaatcgccgt catcaatcat
    11821 gcggcggtaa tacaggctgt tgccgtccac ctccaccggc tcttggccga tatattcgtg
    11881 cgggttgtgt tcggtcggca cacgcaggcc atctgccgcc gttactttga ttttgctcat
    11941 tgcttatcct taagggttag gtttacaggt atttcatcgg cggattgcgg gtcgtaaatt
    12001 ttgccgtcca acccctcgaa atcaggccac ggctcgctca atgtaccctg atacttggta
    12061 aagatatggt tggggtcgtc gggattgtcg gtgcgttcgg ggtagcggtc attttccaac
    12121 ccgcaggtgt taaagcggat ggcatactca accgcatata tgcttactgc ggcgttttgc
    12181 accagcacat gattggcaat cgcgcgcacc gctttgggca ccaagccgcg gctatcggca
    12241 aaaccgagcc gctggccgtc aagtaggcgg cgaacagcgc ggattaaatc gttgctgccg
    12301 atttcgcggc tgtcgatgcc gccttgccgc tgcgcctgct cgttgcgcag attgcgggcc
    12361 gccaccatca ccacaaattc ggcggtatcc tgataacgtc cgcatacgcc gccggtgctg
    12421 gcaggctcaa ctttgctgcc gccatacgtt acccaaaccg caggcagcgt atggatttgc
    12481 cccgccaaat cgtcggcctc gccgttgtag cttttaaccg tgcgcaccat gcgacccaag
    12541 ccccgtttca gacggtctgt tatcgcttgc tcaatatcag tgatcacgcc cgaacacctt
    12601 atttttaccg tttacaaaca caataccgtc ttcgcccgcg gccacttctt cgccgctatc
    12661 atccacgccc agctgcactt cgcctttggc catagcacgc agcaggtcga gcacgtcgat
    12721 tttgtagcgg ttgcggattt cgtcggtaat caacacgccc tgagccgccg tcagacggta
    12781 gcgggcaatg tcgcagcaaa ggcgcaccaa gatgggcggc agatcctcaa aaggtcgtct
    12841 gaaccgcccc agatacgcgt cgatttcggc agtggcgtcc accagcgcgg tttgtgcgac
    12901 ctcgcggtca atcagcccct cgttgttgcg gtcggtgagc tgcaagactt ccagctcacc
    12961 gaaacgcgca accatatcct caaccgtcgc gtatgccatt actcgaccgc cttgcgttgc
    13021 agcataggct cggcgcagat tgccttccac accgcttcgc cgacttcggc gcgcttcact
    13081 tcacgccagc cgccgtcaaa cagcaggccg ccgcgccaaa attctttgcc gtctgcgccg
    13141 gtactgacga gcatcacatc gcggctgtcc gccaaagcgt cggcggcgcg ttgcgtatgc
    13201 tgcactttga gttcggcaag ttcggcggac agtgcctttt tgtcgtcttc ggctttttcc
    13261 aaggctgtgg tcagcatttc gacatcgttt cgggcggcgg caagctctgc ctgcacggcg
    13321 tccaattcgg ctttgatgtc ttcaaacgac ggggcggcgg tttcggcggt ttctggtttg
    13381 ttgttggttt ttgccatgat gactccttgt ttcagacggc ctttaaatag gtgttaaagg
    13441 tcgtctgaaa acggttcggg gttacaggtg gacgcaaacg tgcagtttca gacggccttt
    13501 gaaggtattg gtcgtgccgt tgattttgtc ggcttccaat aattcacggg ctttgtcttc
    13561 cagctcgggc ggaacgacca agaggctggg cttcacattc aacacatagc caccgtcggc
    13621 tttgattttc tgcattttga cgataatggc ggcgaggttt tcgcgggtca gcgcggtttt
    13681 ttccgccatg tgcatcagtt gccacaaacc gaaaccggca ttgcagcggc ggcgcgatcc
    13741 gtacaggtat tcgtcttcca taaacacgcg gtcggatttg gacgggtcga atttggtttc
    13801 aaactcggag tcggtgcggt tttggaaaat cagcggcttc agggtcttgg tgtcatcgac
    13861 gacgtagaag gtcggcgcgt cgttgtccgt accggtcgtc agattggagt gcggcgtatt
    13921 ctgacccgtg ccgtccgatt tctcgtaaac ggggtggtcg gtatcgaaga aatattggcc
    13981 gtcataaccg acagtggttt tacctttttt gagcagaccc cacaccagcg tatcgggcag
    14041 ggcggcggcg gactcgccca ttgccgccat catcgggcgg tacatgccga cttggtcgtc
    14101 ttcgatgtcc gtacgcgcta cgccgacggt tgcttcaaac tttttgttgt cgaggctcat
    14161 cgcctgcttg ttcatttttt cgatttggcg cgagccgacc cattcgcgca tttgggggaa
    14221 tttgccgagc cacgcataag tattggttgc cgtattggac ggaatggtca tcgcaacggc
    14281 ggtatagtcg ggcttgacgg attccaaacc cgtttgaaat tctttgcgga atgctgccgt
    14341 gattgcggtc aaaatcgctg atttgtccat tacttacctt cgctttcttt gatttttaca
    14401 aattcttcgc cggacatgcc cagcattttt gctgcggctg cctcttccgc agtcagtgcg
    14461 gcgacgcgtt cgtcgggtgc tttaccgccc gtttgcgagc ctgccagtgc agcgaccggc
    14521 tgggcgtttt caataaagcc ggtcaaaaat gccaagccgc ccggctgttt caatacgcct
    14581 tctgcccact ccttctgagc aggcagcaat ttgcctgaag tcagcgcggc gactttgctt
    14641 tgcagctctt gaaccactga gataggcgcg tatttagtca aatcaggctt ggcggtttgc
    14701 gccgtcaatg ccgcgatgcg gctgtctttt tccgccagct gcgcgaacac gtcggcagac
    14761 aatgccacgt ctttcggctt ggcttccacg agcgcggaca atgccgcctt cagttcttct
    14821 tcgcccgcat caggcagacc gaacagttgc tgtaacaact ctttcatagg gttttgctcc
    14881 gtttccggtt ttaaaatttg cgccgacgct gccgccagca cctcgtccat accgtccaac
    14941 gcggggaaat ttgtcagcgc ggcgtgaaaa attttgctta catatccctt tgtgtcatag
    15001 gaaaacacag cagagatgta gcgatactct tttgcggcaa ttgccgcagc cgccttgtcc
    15061 gtccactcca cttcggcaaa catgccttta ggcgtgaact ccagccaacg catccaaccg
    15121 gcggcaggtg caggttgtcc gtttttctct ttgtagagcg tctggtgttc ataatcgaca
    15181 accaactgat tgcgcgagct gttggccaac aacgcgacat catgaccgtt ttcttcggtc
    15241 agataccacg cagggacatc agtcggacga ccgtctactg cgcgaaattc gccatatggc
    15301 agcagttgga ttcgcccgtc ttttggctgc acctcgaaac tgcacacggc aagtagggtt
    15361 ttttgtgcat ttttggacat gtgtacctct ctcaatacgg tcattttgcc tatttgaaaa
    15421 aacaacaaag agtaggcgtg cttcaaaccc tcccgccatt tttttaaaac ctgtccgcgc
    15481 gatacacaat taatcgtttt caggggcttt ttagaagcgt ctaggattga tttttatatc
    15541 ttgggcaggg gtttgtatta cccgcataaa aaaacgcgct aaaaagcgcg tttttgaggg
    15601 ttttataaat tcacggtatc agacccgaaa aataatcctg cacatcgtcc atcaaagcct
    15661 gtttgtcgtc gtccgtcagc gtcaaaaatt cccgttgcgg aatccgaact ttgcggttgc
    15721 gccccgccat accgccgaag ttgtggatgg cggcatagac gatattcgta ccgacaaggg
    15781 ctgtatcgtt gtctgacagt gtggaaaaac tgtctttcag acgacccgaa tccgaaagcg
    15841 gcttgccgtc gcggtatttt agccccaacc atttcggacg gcctgcgtag cggaaattga
    15901 gcttgaccgc cgtgtgcatg gtttcggaca gtcggcgcat cagcaggtag cggttttcga
    15961 tcccgttgcc aagccgctcg atttggttta ggacgacaaa gatattgtct attttgacat
    16021 caatcatggt attatcctgc ctaggctact aaaaaagcgg tgaatttgtc tgccctccgc
    16081 agcccttatg cagtcctcgg actgggattg gtatccgtta cataagcgag agtgtgtcgg
    16141 gattgcagtc cgacttagta gccttttttc atttcatagc gtttcttact tttcacaatt
    16201 tctttttcat ccgccctgcg aaacgaatcc aaaaaaatcg aatcgtactg tggcagatat
    16261 ttcaaaactg ccatcaatct gtatttttcg ttcccctcca acggttttcc gagcaaaaca
    16321 aaactttctt ttcctctgct tttgtcataa aaaatatatt cgggctcatg aatcagatcg
    16381 gggagcatgg catataaatc tgttccaaac gattgcccct cccgactgtc cacctgtttg
    16441 accaatgtat catcagacag ccacaccgtc gctcgagtca tacctgccag ttcttgcatt
    16501 tccttgctca atacacccgc agcaaattta agctgtcttg atagcgcgtt tcggattttg
    16561 attttctgct ctttatcggg cttgccatca atattcaaac gttgcttgac ttcataaaac
    16621 tctttttcaa gctgtttaaa gctggtttta aaatccgcac cacccatttc cgctttggca
    16681 aattgatgcg ccaacgcacg gtcgtacttg tctaaatcgg ggcggtagtt cattcgtccg
    16741 gcgttgtaat caaatcctcg atcggtcgtg tacagactgc catccggtgc tttataggca
    16801 agggtcagat aagtatcgcc tttcttattg tagattttat gggtctcaac aagattgtcc
    16861 gacgtgcttt gcccgacaat ccgcccctgg cgttccacat cccgctccga cagcgcgatg
    16921 accgagcaac ggcagttgta gccgttgggc gggtaaaacg ttgcccaaaa cgggtcgtcg
    16981 tagcggtaca ccagcccgtc tattgccgaa tgcgccggac gggtgcggct gtcgcctacc
    17041 gcgtcataca tccaataagg tcgtgcatca atatttgcca tatatccttg atattgaccg
    17101 gcgttgtagg cagtttgcat gttggtacgg taaatcgtct ccaacctccg cggcgaaccg
    17161 aatacctcgc cggtggctgg gtcgatgata tccttaccgt tatgcccgtt cggatgcagc
    17221 cagcccttgt tgctgagcag attcaagata ccttttcgcc aatcgtcaaa cgactgtcca
    17281 ctttttgccg attcaaccat cgaagttttg atgtcgttga gcatatccaa gtcggtcatg
    17341 cgggcaatcg tatagacttt ggcaatttcg gaggctgtca gattgcggta gctctccgcc
    17401 gtaacctttt tactttccag ccactcgatt gccttttttg gcggcagact taaggcaaat
    17461 cccaaatcag gcgcgggcat ggtcttgtcc caaaatatcg ctgataaaca aggcctgctg
    17521 catataggta cgcagtttcg cgttgtccaa attcggataa agcgcattca gtgcggcatc
    17581 tgcctcctcg tagctgttgc aagcattaag tgcggcaacc gcctgacgca ccatcgggtt
    17641 gagctgagaa ttgaaatcgg gctcaaccag cgcgtcatcc aacgcgccgt ccaatatttc
    17701 ctgatgcctg cccgtagcct tagatggtac ggtgtgggcg gataaagccg ccaatgcagt
    17761 tctgtttatc ggattgtccg gtacctgccg caccaacaca gcctcaccct cctgcacatc
    17821 tggaatgacc agtttgtcgc gcacccagct ttcggggatt tgtacgccga catccaccag
    17881 tttcgggata gcgtcggcaa agaccgcgat gtctttcggc tcgcgcgtgt caaattcaaa
    17941 tttcggcacg cggtttgggt cggcattggg atagttgatt tgcaggaacg gtccgatgat
    18001 ttggcttgcg atggtttgcg ccacctgttt tgcgtccgac accagcaaat cgcggcgtac
    18061 ctcgttgtgg atattgccca gcgcgttggt gctggatttt ccgtccgcac cgctggttag
    18121 cgtttgcccc aaaatcagcc gcgccgccga tttttcgcac cagtcggcca tctgcaaaaa
    18181 cggattgctg gttgccatcg taccgcttgc agcgttgtgc agctcgattt ccataccttc
    18241 tggcatgatg cctgccgcgt tgtgaccgat ttccgccacc gctcgaagca gggtgttttt
    18301 ttcctctttg gttgcgcccg cgccgtattt gccgatacgg atgggcatgc cgtacagctc
    18361 caaaaactcg gcaaaatcgt ggacggcgta gtgtttgaac atatacagcc aggaaagcgt
    18421 gcggaaaagc ccgttgcgcg cctgctggag gctgcgcgat ttttgggtat gaacgaccca
    18481 gcccagcggc cacaacgctt cgccttccgg attttcgcgg gtacgcagca gcagcccgtt
    18541 gtctttgtcc catttgaacc agctttgcgg gcggtggata aagtttcggg gtaggtaaag
    18601 gccgtctgaa aatacccact cgacctccaa cgcagaaaat ccgtgcccta ccgcgtccat
    18661 caaatccata atcaggtctt cgagggtagg caggctgtcc atcatttcgt aggcttggtc
    18721 ggacagcttt tcttcttcgg gcgtcgcatt tcgcggcggg gcgacgcgcc agttgagcgt
    18781 cagcagcgcg cgtttgcgcg tccccatatt tgccgcgatg tcgctgtcgc gctcctcaat
    18841 gtccgcgaaa agctcgtgtt gggcgcggat gtcgccgctt tccgcatcct cgaagagagc
    18901 ccgcatcttt tgcggtgtaa taaaattgga cggatgctcg gcgataaccc gaccggtcgc
    18961 cgtaatttga gccacgtccg tctgcaatgc agcttcgggc ttttggattt tagttttatt
    19021 gttctttttt gccattatcg gctccattta ctttttatct cgtcatcaaa atcgttatcg
    19081 gtagggcttt gccattctat cggcacacaa ttggccaccg cccccgacca gagcatatgc
    19141 actgcatcag gaccatcatc atgatcagcc tttggaaaat ggcggaactg ctgtatcagc
    19201 gtctgttggc tctcattcaa cagaatcaaa ccgttcgcca tgtgaggctg taaagtctca
    19261 atccgcaaga gcttgtccga taccggcttg accgcccgcg ccgggacagg tattccacgc
    19321 gccgcgctgc gcttgaccag ctcatctttc aaaaattcct gaaactgaac cgtctcaaca
    19381 aaccacagtt tgcaacgata ttgacggtgc aatctgataa cgtcctcaat aatcaaatca
    19441 gggagacgtt ttttaatctg agtttccacg acatacagtt tgccggtttc acgttgataa
    19501 ccgccaatga taatcgccga cgggtcacgg ctcgcccccg ctttacccag cgacgggtcg
    19561 agcgcaccga aatacaccaa atcggacggc aggtcgttcc aaaacttcat cgacttggca
    19621 aacggcgcat cttcgccgct gaccgggtcg ttttgatatt cgctgtcaaa tgtcgcatga
    19681 ccgtcacgcg cacggatttt catcagcgcg agtacgccgc gagccgccca gcttgtttgc
    19741 gcaccgcgct ccatctcgtc tttattggcg agataaaacg cctcggccac agtctcgccg
    19801 tcgtttcgga aaagttcctc ccatctgtcc cacaaatcca tgcggtcggg ccagcgtttc
    19861 atcgccttaa acttaatacc gtgccaaaac gggttattca aagtgcggtt aagtacgctg
    19921 tcgtaatgca aaatcgtgcc gatatatatc acatcgtatt tctggccgac accgcccaaa
    19981 ggcaatacag tcttagtcag ccacgcattg agcttgtcgc gctgttcggg gttgcggact
    20041 tgctcgtcat tctcaatatc gtccaaaaca gttaagtcag gacggtaagg gccgtgacgc
    20101 aaaccgcgca gctttttacc gctaccggcc acttggactt taacgtcatt ggccgtcaca
    20161 atcgtaccgg cctgccatac gcggccctgc ccgcatactt ccggaaagtc ggttttcaag
    20221 cgcggattaa attccaattc cgccttgatg gcttcgagca tcgggtatgc ctgatctatg
    20281 ctgtccatca caataacggc ataatgtttt tggcctgtca caatacacca cagcgtaaac
    20341 aactgagtaa cctgcgtcga cttaccctcg ccacgcggcg cacccaccgc ctcattttcc
    20401 cctttggggg agcggataat ctccggcaga cggctgaata aaaacgcatg cagttcggat
    20461 ttttcagggg agcggatata atgcggaaag taggtgttga cgaaatactc gtaaccgcct
    20521 accgggtcaa acaccttggc acggcgtgca gcaatagcct tgggcgacgc atcgaagccg
    20581 tccacctctg cctcaataat ttggcggagt tgggcggcat attcggcaag tgactttaaa
    20641 aactctttgg acttcattca attaccttat gcagttgcta cctgtatttc ttttcaattt
    20701 ccacacccaa cggctcgacc agctcgacat aagcctgcaa gtgttgcggg tatcgctccc
    20761 tgaccacttc gccgaacaat tccaacacct cgattgccgt cgccagtttt gacgtttccg
    20821 gcatcacttt ggcgtttgcc gccacggtct tggtaaacgc gtcggacagg cttgtcaaca
    20881 gtttggcgcg ctcggacggc atcagccctt caatcgacgt gtcttgcaac attgtcatcg
    20941 ttgactggta ctgcaccaaa aaacccgcca gcagcgaacg gctcaagtct tcgatgccgc
    21001 cgcctgccaa ggtataggcg gcgcgcactt tgtcccaatc gtcgctgctt tccttggcgg
    21061 cgcgtttcca gctgcgggcg gtcgcaatcg ggatttcgca catcatcgcc gcgatttcaa
    21121 gcgtctgccc gtcgctgacg tacaatcggc gcagcttttc gcggatttcc ttcggatgcg
    21181 ccatatcagc ccccgaactt ggctctcagc agctcccagc cggtcgttac aatcacaccg
    21241 ccgagaccgc cgtaaaccgc cgcagatttc ttgcagtctt tcttaatttg ctgcaattcc
    21301 tcgtccattc gagcctgatt ggcgagcatg tcatcctgct tggcttcgat acgcgccaag
    21361 acttccaaaa tcgggtcgct catgatttgt ccgctttcct gtccaatttt tcgtttactt
    21421 tttccaactt gttttcaatt cgttctaagg ctgccgcaat attagtgctg tctgccttgg
    21481 catcttgctt ggtgtgataa gagagcttga ccgcgtgcag ctcctcttta aggtcgtcaa
    21541 tgcgcttgtc cgcctctttc agacggcctg aaatgccgtt gacccaaaac caaaacgccg
    21601 ccgttgcaat cggccacagg gttctgaaac caaattcaaa gtccatttaa aaccccttta
    21661 aaccggcaca tcgccaaata cgatacggac cgcgtagcct tcgggatgac gactcgccgc
    21721 ctcgaccttt tggccgtctg aaaagactga gtaatatttc cgcaaaatac caatcacatc
    21781 agcaggggcg tcgcggaaaa ctccacacaa aaggtcgtct gaaaatcctt atccatgcgt
    21841 accgcgtaat cgataccggc cttatccaac aaatcggaaa catgaatgac aaacggctct
    21901 tgctcgcgtg cgcggctcaa tcccagctct aaatccgcat ggcggcaggc gaccgtgcgt
    21961 tgcaccaact cacgataagt cgtcatcgcg caccctccga accgtcaact gccgcttgac
    22021 tgttgaccca gtcgcgccaa gcctgatttt ggttttcaag ttcggcaaca tagccgccaa
    22081 actcggcggc gtgttccaac agcgtggccg tcttgccgtc tttcggcgga ttcgggcgta
    22141 ccggcgcaac catcaatgcg gcaggcggtg tcggcatgac cgccttttcg acaaccttaa
    22201 tttccgtagc cgagggcgcg gttgtagagc tgcaagccgt gatggccaaa gccgtcaata
    22261 caaccgccgc ctgcattttt acggtcttga gtaaggacat tttcgatttc ctttttattt
    22321 tccgttttca gacggctgac ttctgcctgt tttttcgcca aagccatgcc gacggcgtgc
    22381 gccttgactt catatttttt agcttccgcg cgcgcctgtt ccagttcgcg ggcgtagttt
    22441 tgagccgaca acagcagggc ttgcgctttg tctttttcca tcttgtcaat gaccgcctgc
    22501 tgcttcgcaa aagccgactt gtagccttga tggtgcgaca ctgccagacc cgtgccgaca
    22561 agcgcgatga tggcaatcgg ttgccagtta ttcgccagca gtttcacgag attcattctc
    22621 gacctcctga cgtttgacgc tgaccaacga acgcgccacc gcatagccgc cgacaatgcc
    22681 caaatacacc gcccaaatct ccgccgacgg gtcgggcaac atcacaaact taaccgtccc
    22741 cgccgcgcag gcaacgtttg cccacagttt cgagtgcgac acattgcctg tcgctgggtt
    22801 tttaaaaata tccaaaatac gcattgctat tccacacttt tggtttgcag gt
//
LOCUS       DAWFIT010000024        22630 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFIT010000024 DAWFIT010000000
VERSION     DAWFIT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.024"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..808
                     /locus_tag="WGQ85_000901"
     CDS             <1..808
                     /locus_tag="WGQ85_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0750356.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMLDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKVIRGNV"
     gene            849..1379
                     /locus_tag="WGQ85_000902"
     CDS             849..1379
                     /locus_tag="WGQ85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0750357.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1390..1529)
                     /locus_tag="WGQ85_000903"
                     /pseudo
     CDS             complement(1390..1529)
                     /locus_tag="WGQ85_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1548..3143)
                     /locus_tag="WGQ85_000904"
     CDS             complement(1548..3143)
                     /locus_tag="WGQ85_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0750358.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WGQ85_000905"
     CDS             complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WGQ85_000905"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0750359.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WGQ85_000906"
     CDS             complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WGQ85_000906"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0750360.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WGQ85_000907"
     CDS             complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WGQ85_000907"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0750361.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WGQ85_000908"
     CDS             complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WGQ85_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0750362.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WGQ85_000909"
     CDS             complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WGQ85_000909"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0750363.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WGQ85_000910"
     CDS             complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WGQ85_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0750364.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WGQ85_000911"
     CDS             complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WGQ85_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0750365.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLNLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAVLTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WGQ85_000912"
     CDS             complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WGQ85_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0750366.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11592..12061)
                     /locus_tag="WGQ85_000913"
                     /pseudo
     CDS             complement(11592..12061)
                     /locus_tag="WGQ85_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12118..13494)
                     /gene="argH"
                     /locus_tag="WGQ85_000914"
     CDS             complement(12118..13494)
                     /gene="argH"
                     /locus_tag="WGQ85_000914"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0750367.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGSAPEQVRFQV
                     KRWREMSA"
     gene            complement(13513..14382)
                     /gene="galU"
                     /locus_tag="WGQ85_000915"
     CDS             complement(13513..14382)
                     /gene="galU"
                     /locus_tag="WGQ85_000915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0750368.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14410..15009)
                     /gene="rdgB"
                     /locus_tag="WGQ85_000916"
     CDS             complement(14410..15009)
                     /gene="rdgB"
                     /locus_tag="WGQ85_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0750369.1"
                     /translation="MSEKTEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYSTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVLHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(15002..15235)
                     /locus_tag="WGQ85_000917"
     CDS             complement(15002..15235)
                     /locus_tag="WGQ85_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750370.1"
                     /translation="MNRRKIYLLSIALFTLAFMLLVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15343..15876)
                     /locus_tag="WGQ85_000918"
     CDS             complement(15343..15876)
                     /locus_tag="WGQ85_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0750371.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15978..16436)
                     /gene="nudB"
                     /locus_tag="WGQ85_000919"
     CDS             complement(15978..16436)
                     /gene="nudB"
                     /locus_tag="WGQ85_000919"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0750372.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLAEGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16647..16723
                     /locus_tag="WGQ85_000920"
     tRNA            16647..16723
                     /locus_tag="WGQ85_000920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16681..16683,aa:Pro,seq:ggg)
     gene            16881..18767
                     /gene="mafB"
                     /locus_tag="WGQ85_000921"
     CDS             16881..18767
                     /gene="mafB"
                     /locus_tag="WGQ85_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0750373.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            18798..19217
                     /locus_tag="WGQ85_000922"
     CDS             18798..19217
                     /locus_tag="WGQ85_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750374.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            19254..20228
                     /gene="mafB"
                     /locus_tag="WGQ85_000923"
                     /pseudo
     CDS             19254..20228
                     /gene="mafB"
                     /locus_tag="WGQ85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            20239..20700
                     /locus_tag="WGQ85_000924"
     CDS             20239..20700
                     /locus_tag="WGQ85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750375.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            20726..20974
                     /locus_tag="WGQ85_000925"
     CDS             20726..20974
                     /locus_tag="WGQ85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0750376.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            21001..21420
                     /locus_tag="WGQ85_000926"
     CDS             21001..21420
                     /locus_tag="WGQ85_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750377.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            21498..21581
                     /locus_tag="WGQ85_000927"
                     /pseudo
     CDS             21498..21581
                     /locus_tag="WGQ85_000927"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21578..21820
                     /locus_tag="WGQ85_000928"
     CDS             21578..21820
                     /locus_tag="WGQ85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0750378.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            21898..22143
                     /locus_tag="WGQ85_000929"
     CDS             21898..22143
                     /locus_tag="WGQ85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0750379.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            22146..22535
                     /locus_tag="WGQ85_000930"
     CDS             22146..22535
                     /locus_tag="WGQ85_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750380.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5393 a         5104 c         6438 g         5695 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcagatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgctgga
      181 tgtggtgttt ttaaatgcag ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttaa tagggatata tcaagttatc tttattcctt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcagaat
      781 tttaaaagta atcagaggga atgtttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaaggat
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gaatcggcaa ccgcgtcttg gtcggtatgg gcagcaccgt tttggacgat gctgtgattg
     1201 aggacgaagt gatgattggc gcgggcagcc tcgttccgcc gcgcaaacgc ttggcgggcg
     1261 gctaccttta tatcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg acgggatgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaagcccttt tttcgggttt agacggcatt ttatcgccga ttgcttttta cagtttgacc
     1561 gaatgttcgc gcgtttcgtg gaacacgatg tccggccaac gttcttgcgt gagtcccaga
     1621 ttcacgcggt taggggcgag gtaggcgagg ttgccgcccg cgtcgatggc gaggttgccc
     1681 gcgttggctt tttcaaattc cgccagtttt ttcttgtcgt cgcacgatac ccagcgcgcc
     1741 gaccagatgg atgcgctgtc gaacacggct tctacgccgt attcgttggc cagccgcgag
     1801 gtaacgactt caaattgcaa cacgccgacc gcgcccaaaa tcaaatccgc gccgctcatc
     1861 ggtttgaaaa cctgtaccgc gccttcttcg ccgagttgtt gcaaaccttt ttgcagttgc
     1921 ttgattttca gcgggttttt gatgcggacg ctgcggaaca gttcgggcgc gaagaatggg
     1981 atgccggtaa acgtcagttg ttcgccttcg gagaagctgt cgccgatttg gatgttgccg
     2041 tggtttggga taccgataat gtcgccggcg taggcttctt caaccagctc gcggtcgtgg
     2101 gacatgaagg ttaccacgct ggaggcggcg atttcgcggt tgatacgcag gtgtttcatc
     2161 ttcataccgc gctcgaatct gccggagcag acgcgcaaga aggcgatacg gtcgcggtgt
     2221 ttcggatcca tattggcttg gattttaaag ataaatccgg aaaacttagc ctcgtccggc
     2281 tcgaccatac gtacggtcgc gtcgcgcggt ttcggcgcgg gcgcccagtc aattaatgaa
     2341 ttgaggattt cctgaatacc gaagttgtta atcgcagagc cgaagaatac gggcgtgagt
     2401 tcgccggcga ggaattcgtc gagattaaac tcgttcgatg ctgcctgcac caattcgatt
     2461 tcgtcgcgca actgttggat ttccaatgga aagcgttgtt ccaattcggg attttctatg
     2521 cctttgatga tgtcgaactc gtgcggcagg cgttcgccgc ccgcttcaaa gagatagatt
     2581 tcgtcgttca ggatgtggta tacgcctttg aagtttttgc ccataccgat cggccaggtg
     2641 acgggcgcgc agcggatttg caggatgttt tccacttcgt ccagcaattc cagagaatcg
     2701 cgtacttctc ggtcgtattt gttcatgaac gtaacaatcg gcgtatcgcg caggcggcag
     2761 acgtttaaga gcttgatggt ttgcgcttcc acgccttttg ccgcgtcgat gaccatcaac
     2821 gcgctgtcca cggcggtcaa aacgcggtag gtgtcttctg agaaatcttg gtgtcccggc
     2881 gtgtccaaga ggttgacggt gtggtcttta tagtcgaact gcatcacgct tgatgccacg
     2941 gaaatgccgc gctgcttctc gatgtccatc cagtcggaag tggcgaattt gccggttttt
     3001 ttgcctttta ccgtacccgc gctttgaatc gcacctgaaa acagcaagag tttttcagtc
     3061 aacgtggttt tacctgcgtc agggtgggag atgatggcaa acgtgcggcg gcggcgcact
     3121 tggtcgagga tttcttggga catggttttc tttgcaaaaa ggttcaggcc gcttttcaga
     3181 cggcccggac agtgtttgag acggcgaaat tgtacaaaaa aatgcctgat aattcaatgt
     3241 tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg tctttcagga cggcatcggc
     3301 ggtttcccac gcgctgccac gccagacttt gtaaaagcag ctttctcgcc cggtgtggca
     3361 ggcgatgccg ccgttttggg cgatgagcat cacaatggcg tcgccgtcgc agtcgaggcg
     3421 cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc ttcatccatt gtttttggcg
     3481 cgaacggctg taatagtggg caaagccggt ttcgacggtt ttttgcaggg cttcggcgtt
     3541 catccacgcc accattaaaa tacgtttggt ttcggcatct tgggcgatgg cgcaaaccaa
     3601 acctttttcg tcaaatttga cggcttcaag caggttttta tccatatttc ctttcagacg
     3661 gcatagtcga ggcggtcaga ggcgcacttc gatgccggct tcgcgcatag cgcgtttggc
     3721 ttcgcggatg gcgatttccc cgaaatggaa aacgccggcg gcaagtacgg catcggcttt
     3781 gccttcggtt atgccttcaa tcaggtgccg gacattgccg accccgccgg aggcgatgac
     3841 ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc aggttgaaac cctgtttcgt
     3901 accgtccctg tccataccgg taagcaggat ttcgcccgcg ccgcgttttt gcatttcgat
     3961 cgcccattcc accgcatcca aaccggtcgg attgcgcccg ccgtgggtaa agatttccca
     4021 gcgtgtgttt ccggggttga cggctttggc atcgacggcg gcgacgatgg cttgcgaacc
     4081 gaaaaatccg gcggcttcgt caatcaggtc gggacgggta acggcggcgg tgttgatgct
     4141 gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg gcaacggtgc gtacgccgcc
     4201 gccgacggtc agggggatga agacttgtcc ggcaacctct tcgatgatgt gcaggatggt
     4261 gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc aattcgtccg cgccttcgcc
     4321 gttgtagcgt ttggcggctt cgacggggtc gcccgcgtcg cgcaaaccga tgaagttcac
     4381 gcctttgacg acgcgcccgt cttttacgtc gagacagggg atgatgcgtt ttgccagtgc
     4441 cataatcgga tgcctttagt cgagggaatc tgccagttgc tgcgcttggg caaaatcgat
     4501 gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct acgccatgtt tttcggcggc
     4561 acacagggcg cggatgtcgt ccaagccggt cagtccgccg gaggcgatga cgggaatgcg
     4621 gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg ccgctcatca taccgtcgcg
     4681 cccgatgtcg gtgtagatga tgctgttgac accgtcgtct tcaaagcgtt ttgccaaatc
     4741 aattacatga tgcccggtta cggttgccca gccgtcgatg gcggccatac cgtctttggc
     4801 atccagcccg acgataatcc tgccggggaa ggctttgcac gcctcgcgca cgaagtcggg
     4861 gtttttgact gccgccgtgc cgataatcac gtcgtttaag cccaaatcca aatattgtcc
     4921 gatggttttc aaatcgcgta tgccgccgcc gagctgtacg gggatgtctt tggcgacggc
     4981 ggcaaggatg tctttgatgg cgggcaggtt ttgcggaaca ccggcaaacg cgccgttcaa
     5041 atctaccaga tgcaggcggc gcgcgccttg tttgaaccag tgcagcgcgg tttcggcggg
     5101 cgaatcggaa aagacggtcg cctcttccat cagcccttgt ttcaggcgga cgcagcgtcc
     5161 ttctttcaaa tcgatggcgg gtatcagcag cataattttt ctccttgtgc ggggccgtgt
     5221 ccggcttacc agtttaaaaa gtttttcaac atcgtcagcc cggcatcgtg gcttttttcg
     5281 gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga tgcaggcaaa cggggacggg
     5341 tagtcgcttt cgcccaatat ggtttcggga ttttcggggg cgaaatagta gctgtggacg
     5401 aagtaaaaac gcgtgtcttg gggaatatct ttaaacagcg ggtggttttg ggtttggcgc
     5461 acggtgttcc agcccatatg cgggactttc agacggcatc cctgcgggtc gcggaggtcg
     5521 cgctcaaagc gtctgacttt gccgccgaac cagcccaagc cgtcggtgtt tccttcttca
     5581 ctgtggtcga ataaaagttg cgcgccgacg cagattccga aaaagggttt gttttttaag
     5641 gcatctttga ctgcctcgtc caggccgccg cgcgtcagtg ccgccataca gtcgggcatc
     5701 gcgccctgcc cgggaaaaat gactttgtcg gcgcgggaca cgcgttcggg gtcgccgctt
     5761 aaaaagattt cggtattttt tccggcaagc tgccccgccg tccggacgga tttcaatacg
     5821 gaatgcaggt tgcccatacc gtaatcgata atggcggttt gcatggcttc ctcctctttt
     5881 tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa atgccgtctg
     5941 aaaacgggag tctgtcttca gacggcatag ggtttaaacc cggaaagccg tttgtcagcc
     6001 ttccatttgt tttgcctgaa cggcagtcag ggctatggtg aacacgatgt cttccaccag
     6061 cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct
     6121 taagacgttg gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg
     6181 gaagaccaaa acggttgcct gtcctgccac cgggctgccc ggggctttgg atttgcccac
     6241 gcccggcacg gttgccgcat catattgcag cgggccgtcg atggcgaggt ctgggcgttt
     6301 ttcccgggca agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt
     6361 gacggtggag taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc
     6421 ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca
     6481 gtcgccgaag acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct
     6541 tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg
     6601 aaccgcaccg gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac
     6661 cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag
     6721 ttcgcacatc ggtccgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga
     6781 gtcgggcagg ctgataccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag
     6841 caggacgcag cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg
     6901 ttctgcgcct tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt
     6961 gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc
     7021 tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc
     7081 aacggaggct gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa
     7141 gaactgtttg gcaaggttca cccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg
     7201 gacggcgaag acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag
     7261 gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg aggttggcgg catcgagtgc
     7321 ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt
     7381 ttcggcggtt gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg caaaggcttg
     7441 tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat
     7501 atatcctttc gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg catataggca
     7561 gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat
     7621 agcaaacaaa ttctgtttcc aacaagataa atatccgcag gtttgtggat gctgccgcct
     7681 ttcagagggt atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa
     7741 tgccgtctga cagggtgggg gcggaaggga ggttgagcgt gaggacggtt tgtccgaccc
     7801 ggagactgat ttcggtatgc cgcgctttgg gcgtggtttt gagaaccacg gcgtgaatgg
     7861 aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc cggacggatg agcagtgtgc
     7921 cgcgtgtgcc tgcgggtgcg ccgctttgaa cgggcaggcg gcccaatccg caatcggcgg
     7981 tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat
     8041 caaggtcggc aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct
     8101 gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga
     8161 aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc
     8221 gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg
     8281 cgagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc
     8341 gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt
     8401 gcgccgtctt gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt
     8461 gggggaacag cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt
     8521 tggtattttt cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt
     8581 caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa
     8641 agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt taaaaccggg gtgttttgaa
     8701 aacttttgga caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg
     8761 ttatttgaaa gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat
     8821 cagcgcgtaa ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt
     8881 ggagagtgtg tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag
     8941 tttgaggaag acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa
     9001 cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt
     9061 tgggagttgt tccaaggaag tccttagggt ggtttgcgcc atcggcaggt aaagcatgaa
     9121 ataggcaagg atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa
     9181 ataaaccaag gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct
     9241 gtctatccaa acggttaaaa aattgcgata gcggaccgat gcccaaacaa ggggcaaggc
     9301 acataatata gtcaaaaccg cacctaaagc cgatacgctt aaggaacgga taaaggcatc
     9361 aaatacggat acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac
     9421 gccaaagggg ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccaat
     9481 ctgaccgggg aggttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg
     9541 gtaaatcttg gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc
     9601 cattaaaaca gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg
     9661 gaaaatggcg gtggtaaaag tggggtagtt caaaatggat accgcgccaa attcgaccag
     9721 catatgcagg gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat
     9781 ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga
     9841 caggctgatg cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag
     9901 gctcataatc atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt
     9961 gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt
    10021 tttgccgaag aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga
    10081 aatcagggta acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg
    10141 gcggaacaac agttcgacgg cgcggttgat gccgacctgc cacgaacgca tagcgacata
    10201 taaaaaaggc agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg
    10261 tattttttta ggagacatag tgtttttcat cggcaaaacg ggcggacagt ataaatgtcc
    10321 acccgtttga caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg
    10381 cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg
    10441 gttccaaatt gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg
    10501 tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct
    10561 tgttttggga ggattttaac acggctgcgc cggaataggt aatgagtgcg ccgggatctc
    10621 tgtggcggac gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcgaaag
    10681 cgtgccagta gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa
    10741 gggcgacgga gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca
    10801 atgcggccgc ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg
    10861 aagtgggggc gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt
    10921 tttccaaatc tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc
    10981 tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga
    11041 tggtggaggc gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga
    11101 tttgttcgga atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg
    11161 caagctggtc gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg
    11221 catctgcaac ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca
    11281 gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca
    11341 tatcgatttt ctcctaatga atgagagtgt acaccttgtt aaaacataac ggtgtgtagt
    11401 gtattccttc ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc
    11461 ggttatggtt tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag gcggaagcgg
    11521 cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg aatgcttgag
    11581 aacccctctc tttaaaacag cgtctgaaca gggttgccgg aatagtattg ccatcccggc
    11641 agatacagtt tgtcggggtc ttgccaatat tgttcatcca gactgttcgg cagtgaggcg
    11701 gttttgttgt cgagatgttt tgttatatcc cacatttctt tcaggttttt accttccgat
    11761 tggaggtggc ggatttcttt tctcaatgcg tctgccgccc gatggatcag cattgcgctg
    11821 tgtatcgcgc cgattttttc agaacggaac aagtcttttc ggcggcggga aaaccccaat
    11881 tgcagacaaa ttgcagtatg ccgccgttgc cgatgtcggc ttccgcccgc caaatcaaaa
    11941 ccagctcctg ttcacgtccg tccatgcttt cgagtttgcc gtcatgctgt tcaaaaagtt
    12001 tgtctaccgc ctgccacatc atctgttcga agcggtcggc ttgggtatcc gtatcggtca
    12061 tggtgttctt gtcttttaaa aatgccgtct gaacatttct tcagacggca tcgggggtta
    12121 agctgacatt tcccgccagc gtttgacttg gaaacggact tgttcaggcg cgctaccgcc
    12181 caagtggttg cgcgcgttta agctgccttc gggtgtcagc acgccgtaaa cgtcgtcggc
    12241 aatcaaatcg ctgaaacctt gtaagacttc gagcggcagt tcgctcaaat cgacgccggc
    12301 ttcatcagca tggcgcacgg cttgggcgac gacttcgtgg gcatcgcgga aaggcatgcc
    12361 tttttttacc aagtaatctg ccaaatcggt ggcggtagcg aagccctgca tcacggcggc
    12421 gcgcatattg tcgggtttga cggttacgcc gcgcatcata tcggcgtaaa tccgcaacgt
    12481 gtcgataagc gtgtcggcgg tgtcgaacaa gggttctttg tcttcctgat tgtctttgtt
    12541 gtacgccaag ggttgggatt tcatcagggt aatcagaccg ataaggtgtc cgatgacgcg
    12601 gccggatttg ccgcgcacga gttcgggcac gtcgggattt ttcttctgcg gcatgatgga
    12661 cgaacctgtg cagaaacggt cggcgatgtc gataaagccg aaacgcggac tcatccacaa
    12721 aatcaattct tcggacaggc ggctcaggtg aaccataatc agcgaggcgg cggcggtgaa
    12781 ctcgacggca aaatcgcggt cggatacggc atcgagcgag ttttggcaga tttgctcgaa
    12841 acccagtaat tcggcggtaa tttcgcgccg aatcgggtag gtcgtaccgg caagggcggc
    12901 tgcgccgagc ggcatacggt tgacgcggcg gcggcagtcc gccatccgtt cgttatcgcg
    12961 gccgagcatt tcgacgtagg cgagcatatg gtgtccgaag ctgacgggct gggcgacttg
    13021 caggtgggta aagccgggca tgacggtttc ggcgttttgt tccgccaaat ccagcaatgc
    13081 cgtctgaagg ctttgaatca ggctttggat aacggtaatc tggtcgcgca gccaaaggcg
    13141 gatgtcggtg gcgacttggt cgttgcggct gcggccggtg tgcaggcgtt tgcctgcatc
    13201 gccgattttg tcggtcaggc ggcgttcgat gttcatgtgg acatcttcca aatcggacga
    13261 ccattcgatt ttgccgctgc ggatttcttc gaggatttcc gccatgcccc gacggatgtc
    13321 cgccaaatcg ccttcgtcca acacgccggt ttctttcaac atttgcgcgt gtgccagcga
    13381 gccttgaatg tcccattcgg caagccgtcg gtcgaaaccg atggaggcgg tgtattgttt
    13441 gacgagttcg gaaacgggtt cgttgaaacg tccggaccag gttttgtcgt gcataaggat
    13501 tccttgatgg ggttattcgg tgcggtattt ttccaaaagg cggcggaagg gttcgccggt
    13561 ttcggggtgt ttcaggccgt aggcgacggt ggcttcgagg tagcccagtt tgctgccgca
    13621 gtcgtagcgc gtgccttcaa aggggtgcgc caggacaaat tcgtgatcga gcagcttggc
    13681 gatgccgtct gtaagctgga tttcgttgcc cgcgccgcgc ggcagtccgg tcagtaagtc
    13741 gaaaatgcgc ggggtaagga tgtagcgtcc gacgacggca aggttggagg gcgcgtcttc
    13801 gggcttgggt ttttcgacaa tgccggtaat gcgttggaac tgtttgagct gttcggtttc
    13861 gacgatgccg tatgagccgg tttgcgatgc ttcaacggtt tctacgccca aaatgctgtt
    13921 gccgctgcgc ccgtacactt cgaccatttg tttgagcgcg cctttggggg catcaatcag
    13981 gtcgtcggca aggataacgg caaagggttc gtctccgatg gcggcgcggg cgcacaagac
    14041 ggcgtgtccc aagcccagtg cttccgcctg acggatgtag aggcaggtaa tgttcggcgg
    14101 caggatgttg cggacgtgtt ccaacaattt gtctttatgg cgcatttcca actcggtttc
    14161 gagttcgtat gccttgtcga aatggtcttc gatgctgcgt ttgttgcgtc cggtaacaaa
    14221 caccatttcc gtgcagccgg cttccacggc ttcttctacg gcgtattgga tcagcggctt
    14281 gtcgacgatg ggcagcattt ctttcgggct tgccttggtg gcgggcagga agcgtgttcc
    14341 catccctgcg acggggaaaa cggctttccg tatgggtttc attctttttc ctttgtattg
    14401 ttttgatgtt taaagggcga gtttgcgtaa gagttcggca agtgcctgcg cgcggtggct
    14461 ttcgcggttt ttgacctccg aatccaattc ggcggcggtt ttgccgtgtc cgggcagata
    14521 aaaatacggg tcgtaaccga aaccgttttc accggcggga acgccgttcc attgtccgtg
    14581 ccatacgcct tcggcaataa tcggacgcgg gtcgtcttta tggaggacaa aaaccaatac
    14641 gcagacgtag caacagcttt tgtcggcttt gccggcaagt tcggcggcaa gtttcaggtt
    14701 gttggcggta tcggatttgg gatggtcgcc cgcgtaacgt gcggaatgga tgcccggcgc
    14761 gccgtttagg gcggcggcac agatgccgct gtcgtcggcg agtgcgggca gcccgctgta
    14821 tttggcggca tggcgtgctt ttgccagcgc gttttcgaca aaggtggaat agggttcggg
    14881 gcattcgggt atgccgaatg cggattgcgg caatacggtg atgccgtaag gtttgaataa
    14941 gttgccgaat tctttgagct tgccggcatt gccgcttgcc aaaacgattt tttccgtttt
    15001 ttcagacata gcggttttcc tttgcggcgg attgggcggc gcgtagggat ttgtgccgca
    15061 ggtagagggc gagtgcgccg atttgcccga acagcgcgcc gaatgcgaaa aggaaggacg
    15121 cgacggcgaa ggctttgctg ccggcggtca gcagataagc acccaagagg acgagcagca
    15181 taaatgccag tgtgaacagg gcaatagaca acagatagat ttttcggcgg ttcatggcgt
    15241 tcggtcggaa acggtatgtt cggattatat ccgattggga cggtattccc tagaggctgg
    15301 aaaaatgccg tctgaaacgg cgttcagacg gcatggcggg cgttatgcct gtttgttcca
    15361 acgttcgatg gattctttga tgactttttt cgcttcttcc gcatcgcccc acgattcgac
    15421 tttggtcgta cctgcttttt tcaggtcttt gtaatggttg aagtggaact cgatttgttt
    15481 gatgagctgt tgcggcaaat cggacaaagt tttgtaggcg ttgccgttgt tgcggtcgtc
    15541 ggcaggaacg cagacgattt tgtcgtctac ttcgccgtcg tcaacgaatt tcatcacgcc
    15601 gataacgcgc gcttccaaga atacgccggt tgccaaaggt tgttcggtaa cgagcagcac
    15661 gtccaattcg tcgccgtctt cgtccaaagt ttggggaatg aagccgtagt tggtcggttt
    15721 ggcgaagatg gcgggttcga cgcggtcgag ttggaatgcg gcgagtttgc ggttccactc
    15781 gattttgtgg ttgctgccgg cggggatttc gttgacgacg ttgatgatgc cgccgtccac
    15841 atcgccgggg gtcaggattt ggttgaagtc tgccatttgg tttcctttgt ttgggaaagt
    15901 ttgaagtttg aaagtatagc acaaacgtcc ggctgaaaat gcgcccgatg ccgtctgaaa
    15961 gggtgtacgg gcgcgtgtta ccgtttgccc aaaaacctgc ccagttccag aatcgcgcgc
    16021 ctgttggacg gggaaaacac tttttccgcc gcttcttcca agccgaacca gccgtaggag
    16081 acgtgttcct cgggttgcag ggcgatgggc gtatcgcgcg ggatttcggc agagaagagg
    16141 tgttcgcggt tttcaaacac gccttttgga tagcggtgcc gccagtggtg gtagatttcg
    16201 taaaccgtgc tgtcgtgcca gtcttgaagc tgtccttccg ccagcagtat gccggtttct
    16261 tcccaaactt cgcgccttgc cgtttgggcg acggtttcgc ccggttcgag gctgccggtt
    16321 accgactgcc aaaatccttt cggatgcgtg cgttcgatga gcaggatgcc gccgtccccg
    16381 ctataaagga cgaccagtgc ggagacgggg tatttgagcg gttttgccat cggcatcttt
    16441 cggcgggctg cggtaatgaa ggggcggatt atagcaaacg ccgcacgtta tggcgtttat
    16501 ccttttccgt atcctttttt ctgcacggat gggacgcgcc ggtgtttgcc ggtaaatttt
    16561 ccgttgtgtc aaaaagataa gggcggttgt gattttaatg cttgccaaag cgtcgggcgg
    16621 aaactataat ccgaaactta tcgagtcgga gtgtggcgca gtctggtagc gcacttgcat
    16681 ggggtgcaag gggtcgaagg ttcgaatcct ttcactccga ccaaaaattc cgaaagccgc
    16741 tttcaaaagc ggcttttttg ccgtccgtat gattatgatg tagagtacgc ggcgacagac
    16801 attcaaatgc cgtctgaaaa ccgttcagat ggcatctctt tatcttagtt tcattccgta
    16861 ccatcttaag gaacatcaaa ttgggcattt cccgcaaaat atcccttatt ctgtccatac
    16921 tggcagtgtg cctgccgatg catgcacacg cctcagattt ggcaaacgat tcttttatcc
    16981 ggcaggttct cgaccgtcag catttcgaac ccgacgggaa ataccaccta ttcggcagca
    17041 ggggggaact tgccgagcgc agcggccata tcggattggg aaaaatacaa agccatcagt
    17101 tgggcaacct gatgattcaa caggcggcca ttaaaggaaa tatcggctac attgtccgct
    17161 tttccgatca cgggcacgaa gtccattccc ccttcgacaa ccatgcctca cattccgatt
    17221 ctgatgaagc cggtagtccc gttgacggat ttagccttta ccgcatccat tgggacggat
    17281 acgaacacca tcccgctgac ggctatgacg ggccacaggg cggcggctat cccgctccca
    17341 aaggcgcgag ggatatatac agctacgaca taaaaggcgt tgcccaaaat atccgcctca
    17401 acctgaccga caaccgcagc accggacaac ggcttgccga ccgtttccac aatgccggta
    17461 gtatgctgac gcaaggagta ggcgacggat tcaaacgcgc cacccgatac agccccgagc
    17521 tggacagatc gggcaatgcc gccgaagcct tcaacggcac tgcagatatc gttaaaaaca
    17581 tcatcggcgc ggcaggagaa attgtcggcg caggcgatgc cgtgcagggc ataagcgaag
    17641 gctcaaacat tgctgtcatg cacggcttgg gtctgctttc caccgaaaac aagatggcgc
    17701 gcatcaacga tttggcagat atggcgcaac tcaaagacta tgccgcagca gccatccgcg
    17761 attgggcagt ccaaaacccc aatgccgcac aaggcataga agccgtcagc aatatcttta
    17821 tggcagccat ccccatcaaa gggattggag ctgttcgggg aaaatacggc ttgggcggca
    17881 tcacggcaca tcctatcaag cggtcgcaga tgggcgcgat cgcattgccg aaagggaaat
    17941 ccgccgtcag cgacaatttt gccgatgcgg catacgccaa atacccgtcc ccttaccatt
    18001 cccgaaatat ccgttcaaac ttggagcagc gttacggcaa agaaaacatc acctcctcaa
    18061 ccgtgccgcc gtcaaacgga aagaatgtga aactggcaaa caaacgccac ccgaagacca
    18121 aagtgccgtt tgacggtaaa gggtttccga attttgagaa gcacgtgaaa tatgatacga
    18181 agctcgatat tcaagaatta tcggggggcg gtatacctaa ggctaagcct gtgtttgatg
    18241 cgaaaccgag atgggaggtt gataggaagc ttaataaatt gacaactcgt gagcaggtgg
    18301 agaaaaatgt tcaggaaacg agaagaagga gtcagagtag tcagtttaaa gcccatgcgc
    18361 aacgagaatg ggaaaataaa acagggttag attttaatca ttttataggt ggtgatatca
    18421 ataagaaagg cacagtaaca ggagggcata gtctaacccg tggtgatgta cgggtgatac
    18481 aacaaacctc ggcacctgat aaacatgggg tttatcaagc gacagtggaa attaaaaagc
    18541 ctgatggaag ttgggaggtg aaaacgaaaa aaggtgggaa agtgatgacc aagcacacca
    18601 tgttcccaaa agattgggat gaggctagaa ttagggctga agttacttcg gcttgggaaa
    18661 gtagaataat gcttaaggat aataaatggc agggtacaag taaatcgggt attaaaatag
    18721 aaggatttac cgaacctaat agaacagcat atcccattta tgaatagtaa tatttatgaa
    18781 aaattaggag attaatgatg aaaagaatta agtgcttttg tgataaattt ccatcaggag
    18841 atacatttag aatgtgtatc attctggatg actatgataa tagggttgat tattatgtag
    18901 gaatatatga ttacattacg tctaccttaa tgagcgatat ttactatcga tccacgattg
    18961 atgagcattt caagattata gaattaatag aaaataatcc aaatgaaatt tatgatgatg
    19021 gcggtggtca acaattttgc ctagaatttc atcatgataa ggtcattttt tatcacaatg
    19081 aatttgatga agaagatggt tatccagtat taagctgttc gctgcatact tttaaaactg
    19141 ctttaattgc ttggaatgct tttttgcaat tgcctaaaag tattcattcg gtggtggaga
    19201 ctgtgattga ggaataagca taattagctt aatgaataga atcagcgata tagaaaggta
    19261 gaatcagata tatcccacct aaaaattact ctccttcagc accgctacca aaaggaccta
    19321 ataatggata tagtgccggg aatttaagca aaaaagtcct gactttagtt tacataaacg
    19381 gcaaactcaa attcgggcca agcagtctgc cgtcttcggc attgaaagca cctgttccca
    19441 ttgtcggcaa ggctgaggct ccgcccggaa aagctgtgcc tgtcggcgaa gcccgcattc
    19501 cttcaaatcc aaaagcggta aaaggaacaa ccgttaccct gtcgcaggaa caacgcctag
    19561 ccaacggaga gctgataccg aaaggctctg tggtaacgtg gtcggacaat gcggtgaagg
    19621 ttgtgcggcc tgatgggaaa aagcggagat ttaatagtcg ggattgggaa cagaaggcat
    19681 tgccgaaacc gcaactaccg aaagattaca gagatgacat caacataggt ggaaataatc
    19741 ctagatttaa tcagcaacgg catgaaaaaa tcgaatccca caacccaaac agaatagagg
    19801 agatacgcca acgtaatgcc gtttcttctg cagaaaggca ggctgatgca ttaaaaagga
    19861 tgcaaacgtc tggccgcacc cgcctgcctg ggcagccaca aaaaggaaaa cccggcgctg
    19921 ctagagaaat gtcctcttca gacaatccga atcaaacagc cgaggctttt gcacgtcgat
    19981 atttaggcag gaaacctaca ctaatggaac gagtcgaagg tgaaaaatta tacggaggta
    20041 aatgtgtggg ctgttggaaa gcgcaaatgc cggacggaac ctttgtggtg tataggccag
    20101 caggtaatgc ttctatgacc aagggaacta ctgcgtctgt tgatattaat aacccaacat
    20161 taaataactt aaatattaat ttaaaaggca agccaacaga gttaaaattt aaattccctg
    20221 ctagataagg atgttttaat gaatttagag acagcagaaa atattcaatg cgaatttgag
    20281 tatttagcat tagatggtta ttttccaatg cattttgcat cgcatggtca aggaaacaaa
    20341 gattggcaat ttgcagttga attcatatat agattattaa tatgtcaatt agcaactctt
    20401 gaacctatca actttgaaac gaaaaatgat attttagact tttgccataa tttagcaaaa
    20461 caaagtcctt ttaataatga taatgaggtg tggtatcagg gggaaattgt attaacaaaa
    20521 aaagggatag atttaataaa ggaatatatt ccagaggcgt ttgaagagtg gaatggaaag
    20581 aaatttgaat taaacattcc tttcattaaa acattaaaaa atatatttgt taattatgag
    20641 gtagcatggg atgagaataa tccactattc cccattatat ctttaaacct cggcacctga
    20701 taaacatggg gtttatcaag cgacagtgga aattaaaaag cctgatggaa gttgggaaat
    20761 gaaaaaagga aggggcggaa aacctatgac aaatcattct atgttcccaa aagattggga
    20821 tgaggctaga attagggctg aagttacttc ggcttgggag aaaagaaagg aattagaagg
    20881 tcaaaaatgg agaggtacaa gtaaatctgg tgttgaaata gaaggattta cggaacctaa
    20941 tcgaacagct tatccacttt ataatagtaa atgaagtatt gattaaaaag gtgtttttaa
    21001 atgtataaaa taaaatgttt ttatgatcag tttagttctg gtgaattatt taactactgc
    21061 cagtttttag ataattcttc taatcaaaag ataaatacac gaggaacggt gtatcaatat
    21121 attatttatg ttttaacagg agacctttat ctacaaaaag acatagatga aaatttagaa
    21181 tttatccatc aagcagaaaa taatcccaat gaagtttatt caggaggtgg acaaggtttt
    21241 tgctgggata tttctgctga aaaagtagtt ttctatcaca acgaatttga tgaagaagac
    21301 ggctggcctg atttaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat
    21361 gcttttttgc aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa
    21421 gcataattag cttaatgaat agaatcagcg atatagattg gactgcaaat ccacgcttat
    21481 gcgctgtgcc atgattaaca ggaagagagc aacttggatg ggctagtagg gatggtaagc
    21541 atttaaatat atcaattgat ggaaagatta cacacaaatg actaatttaa aattagattt
    21601 ctactctgaa gttattataa aagattcttg ccctaatgat ttgttagaaa atggtgagac
    21661 tattaaagga aaaaaaggag tggtattagg tataagcgaa gaagatggta taatctatgg
    21721 ctatactatt ttactttttg atataaaata ttgtatatat atagataaga aatatattat
    21781 accgactgga aaaaaattct ctcgcgatga tttttattag ctaatttaag taatttaagc
    21841 aagtttttta gatactgaaa ctggtaatta tgtttatgta agcataagga gttcagattg
    21901 aaggattcta cagggcaaat gaggctggca accaaggatt tggcggaagc cattaaacga
    21961 ggagaagtac gtagttctgc ttttacaaca aagcaactaa aggcaatcga aaaaggagaa
    22021 tctaaaatac caaattacac ttggcatcat catcaagaca ctggaaggat gcaattgatt
    22081 aatgaagact tgcatcatga taccggccat atcggttgga gagcgatgag taaaggaaag
    22141 taactatgtg gaaaatcata aaagaggata gtaatgattt aggatttgca attaaatgct
    22201 tattctctca gtctattgat ttaaatgaat tcaaattatg gattgaacaa gtaatacgag
    22261 atatgcccat cgaggacatc ccgttttata tttttgattt ggcggatttt gatgggggaa
    22321 ttgccgatat tgacaatatt gtaggttttg tttcgagtta cagcctatca aaatcgaaaa
    22381 aaaatgcctt gacaggcatt gccttcttaa gggggataga tgtctatgat ccgcctattt
    22441 caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag aggtttcagc
    22501 atttctttcc gtttgtagag ctgcccccgc tttaaacagt caaaataccg tctgaaagcc
    22561 atttccgccg ctcagacggc attttcgccc ctttcgttta caaaccctta aaatccattt
    22621 acactcaaac
//
LOCUS       DAWFIT010000025        22247 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFIT010000025 DAWFIT010000000
VERSION     DAWFIT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.025"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGQ85_000931"
     CDS             complement(120..1034)
                     /locus_tag="WGQ85_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0750381.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGQ85_000932"
     CDS             1290..2759
                     /locus_tag="WGQ85_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0750382.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGQ85_000933"
     CDS             3011..3382
                     /locus_tag="WGQ85_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750383.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGQ85_000934"
     CDS             3455..4864
                     /locus_tag="WGQ85_000934"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0750384.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGQ85_000935"
     CDS             4910..5704
                     /locus_tag="WGQ85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750385.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WGQ85_000936"
     CDS             5892..6668
                     /locus_tag="WGQ85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0750386.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHSRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7326..9797)
                     /locus_tag="WGQ85_000937"
     CDS             complement(7326..9797)
                     /locus_tag="WGQ85_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0750387.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGQ85_000938"
     CDS             complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGQ85_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0750388.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10977..12257)
                     /locus_tag="WGQ85_000939"
     CDS             complement(10977..12257)
                     /locus_tag="WGQ85_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0750389.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGDIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12410..13129)
                     /locus_tag="WGQ85_000940"
     CDS             complement(12410..13129)
                     /locus_tag="WGQ85_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0750390.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGQ85_000941"
     CDS             complement(13200..13877)
                     /gene="radC"
                     /locus_tag="WGQ85_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0750391.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSRSVCSF
                     RQLGLMP"
     gene            complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGQ85_000942"
     CDS             complement(14000..15349)
                     /gene="gshA"
                     /locus_tag="WGQ85_000942"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0750392.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15609..17018
                     /gene="leuC"
                     /locus_tag="WGQ85_000943"
     CDS             15609..17018
                     /gene="leuC"
                     /locus_tag="WGQ85_000943"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0750393.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17115..17369
                     /locus_tag="WGQ85_000944"
     CDS             17115..17369
                     /locus_tag="WGQ85_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750394.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17431..18072
                     /gene="leuD"
                     /locus_tag="WGQ85_000945"
     CDS             17431..18072
                     /gene="leuD"
                     /locus_tag="WGQ85_000945"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0750395.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18241..19512
                     /locus_tag="WGQ85_000946"
     CDS             18241..19512
                     /locus_tag="WGQ85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0750396.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19528..20259
                     /locus_tag="WGQ85_000947"
     CDS             19528..20259
                     /locus_tag="WGQ85_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0750397.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20293..21363
                     /gene="leuB"
                     /locus_tag="WGQ85_000948"
     CDS             20293..21363
                     /gene="leuB"
                     /locus_tag="WGQ85_000948"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0750398.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21526..22089
                     /locus_tag="WGQ85_000949"
     CDS             21526..22089
                     /locus_tag="WGQ85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0750399.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5355 a         5857 c         5787 g         5248 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcggggacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc actccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac agaaggcagc
     4981 catcgccccc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gtccccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtcc ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acggcagttc ctcagtcagg cgtttgcgcg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcagatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctagt tgccggcgac ctgaatccag gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacagccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 taaccgtttc tccgaaaaca ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gaatgacgac ggtgttttgc cgtagccaaa
     6901 gcagaaaaac atccctattg tcatttcatc atgatatgac aataggatgt taaaaattaa
     6961 ttatcattat atgattgata tataagaaaa actgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc atacacagaa
     7081 ccggtatttg gaaaaaaaat aatccatatt gacatcatta ttccatttat cgcataaatc
     7141 cactttttaa ttttttttcc tcattaatat aatctcttat ttaataaaat caaataccta
     7201 gcaattcgat ctcaaatttt aaacgccaac gtgtttgagc ggtaacaatg catactttaa
     7261 gtttgtaatc cttcatcgtc tcatttttgt tttaatcgac tatacgtcta aagcaacgcc
     7321 gtccgtcatt gttcggacgg cattttttca gacggcattt tcccccgttt gtgaaggcgc
     7381 agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc tatccacggt
     7441 tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc cgcccatatc
     7501 aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg ggcgacggta
     7561 cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc cgtcccgccc
     7621 gctgcggcgg cggatacgtc tgcctgcgcc aaaacgggcg cgtcgttgat gccgtcgcct
     7681 atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag tttgtcctcg
     7741 ggcatggctt gggcgcggta gtgcgtgata cccagggcgc gggcggtttc ggcaacggcg
     7801 gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa ctgccacacc
     7861 gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg gaaaccgctt
     7921 tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt acctgaaatt
     7981 tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt gacggtcagt
     8041 tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcaat gtcggggacg
     8101 ctgccgcctg aaatgcggca gttgaggatg gcgcgcgcga gcgggtgttc ggactgctgt
     8161 tccaaacttt gcgccactgc cagcacttgg gcttcggtca tgctgccgag cagttcaatc
     8221 cggcggacgg cgggattgcc ttgggtcagc gtgccggttt tgtcgaaaat gatgtcggtg
     8281 gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc gcgcgccagc
     8341 gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca ggggcaggta
     8401 atgaccagca gggcgacggt aatccacaat gcggtgtgcg catcggcgta caacgtccag
     8461 ccgatgaaga ccgggacggc aagcagtagt tcgccgaata tgaaagacga ggcgtattgt
     8521 tccgccaact cggcggtgcg cggtttttgc gccaaggcgc ggtcgagcaa gcggacgatg
     8581 tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg gctttgcgtg
     8641 ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag gctctcgccg
     8701 gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc aacggggatg
     8761 gtttcgcccg gtttgaccag cacgatatcg cccgccttca atttgacgac agctgcctcg
     8821 caggtttcct gcgtatcggg gtaatcgggc atatgatggc aaaacgcagg aatcagcttc
     8881 accagccttt cggcagcatc gcctgcctta cggcgggcaa tgtgttccat aaagcgtccg
     8941 cccagcagga aaaacagcag catggctatg gattcgaaat acatcccctg ccccgcattg
     9001 gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc aatcggcgta
     9061 tccatgccgg cgcggcggtt tttcaagtcg cgcagcgcgc cttgataaaa cgggacggcg
     9121 caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat ttgcaggaaa
     9181 tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat cgtctgcatc
     9241 atccccagcc cggcaacggc gaggcggacg atgtattgtt tgcgttcttt ttggttggcg
     9301 gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct gattttcaac
     9361 agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg gtgcgtgctg
     9421 taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc gatcagccag
     9481 acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt gccgccgtgg
     9541 gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg gtcgaggatt
     9601 tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta ataactgccc
     9661 aagcccgcgt caataatgct ttgtgcgacc gcctgacagc cggcgcagca ggtttcgcgg
     9721 tcttcgtttt cgtaacggac agtcaggtgg aggtgttcgg gaacatccag cccgcagtgg
     9781 aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggcg gcacaagact
     9841 cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa atgcggacgg
     9901 cggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca gacggcagca
     9961 ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt tctcgcagta
    10021 cgttttccac gtcttcaatc tgctgtccgc tgttcggagc ggcggcatcg acgacgtgca
    10081 gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaaggcggaa atcagtttgt
    10141 gcggcagatc gctgacgaat ccgacggtat cggtcaggat gatgctgcat tcgggactga
    10201 tgtacagccg ccgcgccgtg gtgtcgagtg tggcgaaaag ctggtctttc gcatatatgc
    10261 ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag ccgaccagcg
    10321 caaacgtttt gattgtgccc gattcgcggg acttgcggcg cagggcgcgc tgttttttga
    10381 ggttggcaag ctgttttttc aaggcgttga ttcgatgggc gatcaatcgg cggtcggttt
    10441 ccagtttggt ttcgcccggc cctttcatgc cgataccgcc gcgctggctc tgcagatggc
    10501 cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg acttgcagcc
    10561 tgccttcctg cgtgcgggcg cggcgggcga aaatcgccaa aatcaaccct accctgtcca
    10621 atacgcggca ttggaggatt ttttccaaat tgcgttcctg cgtgggcgta agttcgtggt
    10681 tgaatacgac caaatcgatg ccgtctgcgg caactgcttc cgacagctcc gccgccttgc
    10741 ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc acgcgcacgg
    10801 aatcgccgcc cgccgctttg accagctcga cagcttccgc caatgccgtc tgaaaaccgt
    10861 tcagacgggt ggcactgctg cccgtgccat ctttgtccaa cattacgccc accagcatga
    10921 cgcgttcggg ttgcgcttgg gtactgccgt tgcggcctgt tctgcctgtc atgtattcaa
    10981 tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag cgtgatgcgg
    11041 ctgcccgcca ttccgtcaac gctgaagacg atgtcgcccg cctcgtgcct tcctatggac
    11101 aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa acaccagcgt
    11161 ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc gccgccgttt
    11221 acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc ctgcgctcgc
    11281 cgtccgttgc ggacatagtc gaataccgcc gtataaagcg tgccgtattc caaaggcttg
    11341 agcggaaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt gccggcggtt
    11401 aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact tttcatcgta
    11461 attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat ttcatattcc
    11521 ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgctgcc gacgggatag
    11581 gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac cgcaccgtta
    11641 tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc ttgggcgcaa
    11701 tgccgctcaa acctgccgtt gccctgattg aatacgagaa cggttttacc gttttcgcgc
    11761 acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg gtggtagatt
    11821 gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc ggacgattcg
    11881 gcggcttctt cttccgtgct gatgttttca tgcacgccgt tgtagagata cccggcaagg
    11941 cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg gtgttcgccg
    12001 tgtccgtctt cgggattgag cgtgaggtag cttgcgtgcc tgcgggcgga gttttccaaa
    12061 accggcgcgt gtgccagctt gtgcaaaccg atttgggctc ggatgcggtt gaggtagtgc
    12121 agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc gcccgcgggc
    12181 agggattggt tttgggtatg gtagaaaacg cctgccgccg aacccaacag gagcagccga
    12241 ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt ttatcaaaac
    12301 ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac ctttccccgt
    12361 gccgatgccg aacccgatgc cgtctgaacc ttcagacggc atcgggtgtt tatttgtcca
    12421 cccggatggg ggcaggttcg tattgtgtcg attcgtcgcc gtaatacagc acgccgagtt
    12481 tgatgggaat gcgtccctgc gatttgcggt gggcattgga atcgcgcaag gaataggcgc
    12541 aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc atgcgcgcgc
    12601 tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa ggcgcggcgg
    12661 cacggcggcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg cccagcgaac
    12721 tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca aaacgcatat
    12781 cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct ttggctttgg
    12841 caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa ggaatgccga
    12901 acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg tgcggatgga
    12961 tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc gcctccatca
    13021 cttcgccgct gcacggggcg caacaggaat gcagcaagac ttttttatgt ccgccgggcg
    13081 ggacgagtat ggggcggtcg atgtcggtaa cgatgcgtgt tgtgtccata gtttcaaata
    13141 gatgttctgt ttggtgcaaa cggctatttt atcagagccg ccgcatgtga aacagagtgt
    13201 cagggcatca acccgagctg cctgaacgag cagacgctcc gggaggttac gataaaatgg
    13261 tcgagcagcg acacatcgac cagcgacatg gcctgtgcca gccgccttgt gaacatgatg
    13321 tcttcctgcg aaggttcggg cgagccgccc ggatggttgt gcgcaataat caggctgtcg
    13381 gcatattcgt ccaatgccag tttgacgatt tcgcggatgt aaatcgtgtt ttccgcaacc
    13441 gtaccgcgcg acagctctct gaccgcaatc agttggtttt ggcggttcag cagcagcgcg
    13501 acgctgactt cgactttttc ctgccccaaa tgaaagcgta aataatcggc aacggtatcc
    13561 ggatcggaca ggacgatgct ctcctgcaat tcttccgcca atatccgccg cccgatttcc
    13621 ctgactacgg caaactgtgt gaaacttgcc gtccccatcc ctttgtatgc cgacagtttg
    13681 ccgacctccg cgctcatcag cctccccaaa ctgccgaact cctgcagcaa ataacgcgcc
    13741 aaatcgaccg cactcattcc gcgcgtgccg acgcgcagca ggattgccaa aagttcggca
    13801 tcactcaaag ccgccgcccc gcgttccaac agcttttccc tgggtctttc gccttccggc
    13861 cattgcttga tgctcatgtt ttcccttttt taaatagtct gagatgccgt ctgaaacaaa
    13921 tgttcccaca cgccaaatat ccgaaatgcc gtctgaaccg gatttccgct tcagacggca
    13981 tctgtatttc aaaacagtct tacgccgttt cttccaactc gattgaagcc gccagaagcg
    14041 acaggcgtgc cattacgccg taaacgtaga ggcggttgtg ttcgttgtcc acgtcgcagt
    14101 ctttttcaga gcgcggcata cccagcgagt cccattgttc gaatacgcgc ttgcaggctt
    14161 cgggcgcttc ttgggagttg tcgccgttgc cggtggggat gctgttggac agcggaacaa
    14221 acaccatacc gccggcgttg aggttttcgt ccgcaccgcg cccttcgtgt acgcggaaaa
    14281 agccgccgat gacgaagcgg tccatcatat acacgacggg ttcgcacacc gcgccgttta
    14341 aggtttcata agtataaatc ccttcttgga caatcacttc gctgacttcc aaaccttctt
    14401 tgactttcgc cattttattg cggtttttac ggttcaatcc gcgcacttcg tcggcggatt
    14461 tgacgctcat cacgcccatg ccgtaagtgc cggcatcggc tttgacgatg acgaaaggtt
    14521 tgtcggtaat gcccgattcg tcgtatttgg cttgaatttt cgccagcaca cgttctaccg
    14581 cttccgccaa cgcgtcttcg ccttcacgcc cttggaagtc caaaccgccg attttttcaa
    14641 aataagggtt aatttgccat tcgtcgatgc cgattaactt ggcaaattcg gcggtaactt
    14701 ggttgtacgc gccgaaatga tttgttttgc ggcgcgtcgt ccaaccgccg tgcaacggcg
    14761 gcaaaacggt ttggctgatg cctttgagga tgtcgggaat gccggcagac aaatcgttgt
    14821 tcaacaaaac cacgcaaggc gaaaagccgt ctgcaagatg gacgcggtcg cgggtacgca
    14881 ataaaggttc caacaggatt ttgtcgccca atgcagtttc aaattcggtc ggttcggtta
    14941 cttccggatt caggctgccc aagcgcactt cataccctgc cgaacgcaaa atctcgccga
    15001 gggcgtaaat gttttgcagg taaaacgtat tgcgcgtgtg gttttcggga ataatcaata
    15061 cggatttcgc cgtttcgcag gcgcgttgca ccgcatcttg cgcggcaacc gccgccagcg
    15121 ggataaagtt gggattcaga ttattgaagc cgccggggaa caaattcata tcgatagacg
    15181 aaattttgta accggcattg cgtatatcga ccgaaccgta aaacggcggg cggtgtacgc
    15241 tccactgcgt gcggaaccac gcctcgattt tggcgtgatt ggacaggatt tttgcctcaa
    15301 acgcctgaag ttgcgccgaa tgttcgggcg acatcaccgg taatttcatc tttcgtctgc
    15361 ctctgtgtgt tttttgacaa gatgcagatg ataatgcctt tgaaagaatg acgacaagta
    15421 ttctttcgta tcgaatcatg ctttagatat aactgtttac tgaaatggac aatacgtcta
    15481 attttttcgt taaaattgtt tgcaaaataa caccggctca aaaattagac aaaatttgtt
    15541 gcgcggtata aagaatacgt ctaaaatccg cccaatccgc attaccattt atccaaaaga
    15601 acaacatcat gacagcacaa accctctacg acaaactttg gaacagccac gtcgtccgcg
    15661 aagaagaaga cggcaccgtc ctgctctaca tcgaccgcca tttggtgcac gaagttacca
    15721 gccctcaggc atttgaaggc ttgaaaatgg cggggcgcaa gctgtggcgc atcgacagcg
    15781 tcgtctccac cgccgaccac aacaccccga ccggcgattg ggacaaaggc atccaagacc
    15841 cgatttccaa gctgcaagtc gatactttgg acaaaaacat taaagagttt ggcgcactcg
    15901 cctattttcc gtttatggac aaaggtcagg gcatcgtaca cgttatgggc cccgaacaag
    15961 gcgcgaccct gcccggtatg accgtcgtct gcggcgactc gcacacttcc acccacggcg
    16021 cattcggcgc actggcgcac ggcatcggca cttccgaagt cgagcacacg atggcgaccc
    16081 agtgcattac cgcgaaaaaa tccaaatcca tgctgattgc cgttgacggc aaattaaaag
    16141 cgggcgttac cgccaaagac gtggcacttt acatcatcgg gcaaatcggc acggcgggcg
    16201 gtacaggcta cgccgtcgag tttggcggcg aagccatccg cagcctttct atggaaggcc
    16261 gcatgacttt atgcaatatg gcgatcgaag ccggtgcgcg ctcaggcatg gttgccgtcg
    16321 accaaaccac catcgactat gtaaaagata aacccttcgc acccgaaggc gaagcgtggg
    16381 acaaagccgt cgagtactgg cgcacgctgg tgtctgacga aggtgcggta ttcgacaaag
    16441 aataccgttt caacgccgaa gacatcgaac cgcaagtcac ttggggtacc tcgcctgaaa
    16501 tggttttaga catcagcagc aaagtgccga atcctgccga agaaaccgat ccggtcaaac
    16561 gcagcggtat ggaacgcgcc cttgaataca tgggcttgga agccggtacg ccattaaacg
    16621 aaatccccgt cgacatcgta ttcatcggct cttgcaccaa cagccgcatc gaagacttgc
    16681 gcgaagccgc cgccatcgcc aaagaccgca aaaaagccgc caacgtacag cgcgtgttaa
    16741 tcgtccccgg ctccggtttg gttaaagaac aagccgaaaa agaaggcttg gacaaaattt
    16801 tcattgaagc cggttttgaa tggcgcgaac cgggctgttc aatgtgtctc gccatgaacg
    16861 ccgaccgcct cgcaccgggg caacgctgcg cctccacctc caaccgcaat tttgaaggcc
    16921 gtcaaggcaa cggcggacgt acccacctcg tcagccctgc catggcagcc gcagccgcag
    16981 tgaccggtcg ctttaccgac atccgcatga tggcgtaagt attttcaggc ggtctttaag
    17041 aatttgacgc ggccgtctga aatatgtccc gtaaggcaga aacaccttta atattcaaac
    17101 caaggagttt aaccatgaac aaacttttca ttaccgccct gtccgccctc gccttgtccg
    17161 cctgcgccgg cacttgggag ggcgcgaaac aagacaccgc ccgcaacctt gacaaaacac
    17221 aggccgccgc cgaacgcgcc gccgaacaaa caggcaacgc cgtcgaaaaa ggctgggaca
    17281 aaaccaaaga agccgtcaaa aaaggcggca atgccgtcgg acgcggcatt tcccatctcg
    17341 gcggaaaaat cgaaaacgcc accgaataac cgattcgcga tgccgtctga aaccgcttca
    17401 gacggcatcg tcagcacaag acgcaaaccc atgaaagcct ttaccaaaat taccgccatc
    17461 gtcgccccgc tcgaccgcag caacgtcgat accgacgcca tcatccccaa acaattttta
    17521 aaatccatca aacgcagcgg cttcggcccc aatgcctttg acgaatggcg ttacctcgac
    17581 cacggcgaac cgggcatgga caacagcaaa cgcccgttga accctgattt ctcgctgaac
    17641 cagccgcgtt accaaggcgc gcaaatcctg ttgacgcgta aaaacttcgg ttgcggctct
    17701 tcacgcgaac acgccccttg ggcattggac gactacggct tccgcgccgt catcgccccc
    17761 agcttcgccg acatcttctt taacaactgc tacaaaaacg gccttttacc tatcgtgttg
    17821 accgaagaac aagtcgatcg gcttttcaaa gaagttgaag ccaacgaagg ctatcagctc
    17881 tccatcgacc ttgccgaaca aaccctgacc acaccaagcg gcgaaacatt cacattcgac
    17941 attaccgaac accgcaaaca ctgcctctta aacggcttgg atgaaatcgg tctgaccctg
    18001 caacacgccg acgaaattca cgccttcgaa gaaaaacgcc gccaaagcca gccttggctg
    18061 tttaacggtt aagcctatgc cgtctgaaga ccgttcagac ggcattcctg ataacatata
    18121 attgctgcaa ctaatgtaaa cagaggggct ataaattgct ttcatctttc aagcgggctt
    18181 agcaacgctc taaattaatg caatacagct ctatttcaaa taaattttta gatttttaat
    18241 atgcaacaga ttaaatttat tgacttattt tccgggatga gcggtatcag gaaaggattt
    18301 gaacaagcct gtcggaaaca gtcggttgct tgcgaatgtg tttttacctc agaaatcaaa
    18361 ccggcagctt tggaagtatt gaagcaaaac taccctgatg aagtgccgta tggagatata
    18421 acgaaaattg aaacgggaga tattcccgat tttgacatct tgttggcagg cttcccttgc
    18481 caggcttttt ctttcgccgg aaaaagattg ggctttgaag atacgcgggg aacgcttttc
    18541 tttgatgtgg caaggatttt aaaggcaaaa aaaccaaaag gttttatttt ggaaaatgtg
    18601 gaaggattgg tgacgcacga tagaaaagat ccgacacaaa aaataggacg caccctgacc
    18661 gttattttgg aaaccttgga aacattgggc tactatgttt cttggaaagt tttaaatgca
    18721 aaagatttcg gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc
    18781 aaacccgact tgtcttttga aacaacccta agtccgaaat taaaaaatat tttggaatcg
    18841 ggactgccta ccgaaagcag tcctttcatc aaaaaattgc taaagaaatt tcccccgtcc
    18901 gaactgtacg gaaaatcagt aaaggacaaa cggggaggga aaaacaatat ccacagttgg
    18961 gatattgaat taaaaggcac agtaaccgaa gaagagaagc aattattaaa tatgcttcta
    19021 aaagaacgga ggaagaaaaa atgggcttca gaaatcggca tcgattggat ggatgggatg
    19081 cctttgacaa aagcgcaaat ttcaactttc tataaacacc ccaatcttca aaatattttg
    19141 gacagcctga cagataaagg ctatttggtt ttggagcatc cgaaacaaaa aattggcggg
    19201 cgaagaatca aagatgaatc cctgcccaaa ggctacaata ttgtttcagg aaaaaaatca
    19261 tttgaaatca ataaaatatt agacccaaat gatgttgcgc caaccttagt tgcaatggat
    19321 atggagcacc ttttcgtcgt tgacaacggc ggtttgcgca cacttaccgg aaaagaaggt
    19381 ttacgcttat tcggctatcc ggacgattat tcgtttgata ttcctaaaaa agacaaatac
    19441 gatttattgg gcaataccgt tgccgttcct gtgattaagg cggtatctga aaggcttctg
    19501 catactttat aaaccgatag ggataaaatg gtaaaactta ctgcacaaca aatatttgat
    19561 aagttgctga atgaggaaaa aatcttatcg gtcaatggtc aaattagatt tttcttgggc
    19621 gatgtggata ttatcgtcaa acaaaaagat gtcgtcggca acatcattca agaatggctc
    19681 ggcggatggt tgagaaaaag ggaaattgaa tttgatgttt caaccaatac ccaaatgccc
    19741 cccgatttct ttttaaataa aaaagaccgc agtagagaat tgttggaagt aaaggcgttc
    19801 aaccgaaatg ccggcccggg ttttgatatt gcagatttta aaatgtattc tgatgaaatc
    19861 attcataaac cctatatgct ggatgtagat tatttaatat tcggttacga tatggacgac
    19921 aacggcaatg taaccatcaa ggatttatgg cttaaaaaag tatggcaaat tacgagaagc
    19981 atggatgggt gggcaattaa tcttcaagtc aaaaaaggcg tggtgcataa aatccgcccg
    20041 ggtgtttggt acagcataaa taaaaagaat atgcctatgt ttgaatgctt ggaagatttt
    20101 gtttccgcaa ttgaagaaac cgtttatcaa aacccggcaa cacggcataa cgcatcctta
    20161 tggaaaaaga aatttgagga agcctataaa aaacactaca accgatcgat ttccatacct
    20221 cgttggcacg aaattgcaca caaatacaaa aagaaataac ccttcaacca tcaacccata
    20281 aggaaacttg aaatgaccaa acatatcgcc atcctccgag gtgacggcat cggtcccgaa
    20341 atcgtcgccg aaaccgtccg cgtactcgac aaactcatcg cccaaggctt ggacatcagc
    20401 tacgaatacg ccccattggg tggtgaagcc tacgacgaat acggccatcc ttatcccgaa
    20461 tttacgcaaa acctctgccg caaagccgat gccgtcctgc ttggtgcagt cggttctcct
    20521 caatacgaca acctcgaccg tccgttgcgc cctgagcgcg gcctgttggc aatccgtaag
    20581 gatttgaacc tgtttgccaa tttgcgtcct gctgttttat atcccgaatt agccaacgcc
    20641 tccacgctga agcctgaaat cgttgccggt ttggatattt tgattgtgcg cgaactcacc
    20701 ggcgacatct atttcggcga accgcgcggc atccgcgttc tggaaaacgg cgaacgcgaa
    20761 ggctacaaca ccatgaaata cagcgaaagc gaaatccgcc gtattgccca tgttgccttc
    20821 caatccgccc aaaaacgcag caaaaaagtc tgttccgtag gcaaagccaa cgttttggaa
    20881 accaccgaac tgtggcgcga aatctttgaa gaaatcggca aacaatatcc cgatgtcgaa
    20941 ctctcccata tgtatgtgga caacgccgct atgcagctcg tgcgcgcgcc caaacaattt
    21001 gacgtgattg ccaccggcaa catcttcggc gatattttat cggacgaggc ctctatgctg
    21061 accggctcca tcggtatgct gccttctgct tctttggacg aaaacggcaa aggcctgtac
    21121 gaaccgtctc acggctccgc tcccgacatc gccggacaaa acaaagccaa cccgctggcc
    21181 accatcctct cgctcgcaat gttgctgcgt tacagcctga acgacgaagc gcgcgcgcaa
    21241 caagttgaaa acgccgtcca aaaagtgctg caacaaggct tgcgtaccag cgacatttac
    21301 gaagaaggca caaaactcgt ttcctgctcc gaaatgggcg atgcgatact cgccgccttg
    21361 taaaaaaaca aagcgcggat cacaccgcgc tttttcatct gcccttttcc gcccgcaccg
    21421 ttcgtcaacc gcctgcaaac ccaccgttta ccggcaggaa atgccataaa tccctgccta
    21481 tgttaggata aacgccttta ccggcccaca caaaggatta tcatcatgaa aaaaatcatc
    21541 ttcgccgcac tcgcagccgc cgccatcagt actgcctccg ccgccaccta caaagtggac
    21601 gaatatcacg ccaacgcccg tttcgccatc gaccatttca acaccagcac caacgtcggc
    21661 ggtttttacg gtctgaccgg ttccgtcgag ttcgaccaag caaaacgcga cggtaaaatc
    21721 gacatcacca tccccattgc caacctgcaa agcggttcgc aacactttac cgaccacctg
    21781 aaatcagccg acatcttcga tgccgcccaa tatccggaca tccgctttgt ttccaccaaa
    21841 ttcaacttca acggcaaaaa actggtttcc gttgacggca acctgaccat gcacggcaaa
    21901 accgcccccg tcaaactcaa agccgaaaaa ttcaactgct accaaagccc gatgttgaaa
    21961 accgaagttt gcggcggcga cttcagcacc accatcgacc gcaccaaatg gggcatggac
    22021 tacctcgtta acgttggtat gaccaaaagc gtccgcatcg acatccaaat cgaggcagcc
    22081 aaacaataaa tcttgatgcg aatcagcaca aatgccgtct gaacgtcctt caaacggcat
    22141 ttgtgccgtc ttcatcaaac cgccccgcca acggtaagcc gatatggcgg agcacaatcc
    22201 gccgaaagat atttcagcgg caggcagggc ttgccggaaa gtccgga
//
LOCUS       DAWFIT010000026        22031 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFIT010000026 DAWFIT010000000
VERSION     DAWFIT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.026"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..303
                     /locus_tag="WGQ85_000950"
                     /pseudo
     CDS             1..303
                     /locus_tag="WGQ85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            357..908
                     /locus_tag="WGQ85_000951"
     CDS             357..908
                     /locus_tag="WGQ85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750400.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(1184..1875)
                     /locus_tag="WGQ85_000952"
                     /pseudo
     CDS             complement(1184..1875)
                     /locus_tag="WGQ85_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(2181..2738)
                     /locus_tag="WGQ85_000953"
     CDS             complement(2181..2738)
                     /locus_tag="WGQ85_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750401.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2982..3140)
                     /locus_tag="WGQ85_000954"
     CDS             complement(2982..3140)
                     /locus_tag="WGQ85_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750402.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(3137..3979)
                     /locus_tag="WGQ85_000955"
     CDS             complement(3137..3979)
                     /locus_tag="WGQ85_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0750403.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDSIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLTQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            4045..4188
                     /locus_tag="WGQ85_000956"
     CDS             4045..4188
                     /locus_tag="WGQ85_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750404.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            4218..5138
                     /gene="pyrB"
                     /locus_tag="WGQ85_000957"
     CDS             4218..5138
                     /gene="pyrB"
                     /locus_tag="WGQ85_000957"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0750405.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            5148..5606
                     /locus_tag="WGQ85_000958"
     CDS             5148..5606
                     /locus_tag="WGQ85_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0750406.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5671..6336
                     /locus_tag="WGQ85_000959"
     CDS             5671..6336
                     /locus_tag="WGQ85_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750407.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6399..7616)
                     /locus_tag="WGQ85_000960"
     CDS             complement(6399..7616)
                     /locus_tag="WGQ85_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0750408.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7758..8303
                     /gene="def"
                     /locus_tag="WGQ85_000961"
     CDS             7758..8303
                     /gene="def"
                     /locus_tag="WGQ85_000961"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0750409.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8390..9316
                     /locus_tag="WGQ85_000962"
     CDS             8390..9316
                     /locus_tag="WGQ85_000962"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0750410.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9395..10654
                     /gene="rsmB"
                     /locus_tag="WGQ85_000963"
     CDS             9395..10654
                     /gene="rsmB"
                     /locus_tag="WGQ85_000963"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0750411.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10620..11219
                     /locus_tag="WGQ85_000964"
     CDS             10620..11219
                     /locus_tag="WGQ85_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0750412.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            11219..13336
                     /locus_tag="WGQ85_000965"
     CDS             11219..13336
                     /locus_tag="WGQ85_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0750413.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            13329..14606
                     /locus_tag="WGQ85_000966"
     CDS             13329..14606
                     /locus_tag="WGQ85_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0750414.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14697..15884
                     /gene="dprA"
                     /locus_tag="WGQ85_000967"
     CDS             14697..15884
                     /gene="dprA"
                     /locus_tag="WGQ85_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0750415.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15910..16371
                     /locus_tag="WGQ85_000968"
     CDS             15910..16371
                     /locus_tag="WGQ85_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0750416.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16443..18749
                     /gene="topA"
                     /locus_tag="WGQ85_000969"
     CDS             16443..18749
                     /gene="topA"
                     /locus_tag="WGQ85_000969"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0750417.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPSRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18822..19517)
                     /locus_tag="WGQ85_000970"
     CDS             complement(18822..19517)
                     /locus_tag="WGQ85_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750418.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(19537..20088)
                     /locus_tag="WGQ85_000971"
     CDS             complement(19537..20088)
                     /locus_tag="WGQ85_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750419.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            20207..20585
                     /locus_tag="WGQ85_000972"
                     /pseudo
     CDS             20207..20585
                     /locus_tag="WGQ85_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            20674..21243
                     /gene="rsmD"
                     /locus_tag="WGQ85_000973"
     CDS             20674..21243
                     /gene="rsmD"
                     /locus_tag="WGQ85_000973"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0750420.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21366..21617
                     /locus_tag="WGQ85_000974"
     CDS             21366..21617
                     /locus_tag="WGQ85_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0750421.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            21714..21797
                     /locus_tag="WGQ85_000975"
     tRNA            21714..21797
                     /locus_tag="WGQ85_000975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21748..21750,aa:Tyr,seq:gta)
     gene            21828..21901
                     /locus_tag="WGQ85_000976"
     tRNA            21828..21901
                     /locus_tag="WGQ85_000976"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21860..21862,aa:Gly,seq:tcc)
     gene            21911..21985
                     /locus_tag="WGQ85_000977"
     tRNA            21911..21985
                     /locus_tag="WGQ85_000977"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21943..21945,aa:Thr,seq:ggt)
BASE COUNT         5474 a         5500 c         6042 g         5015 t
ORIGIN      
        1 attgtttata cggattgtta tcgtagctat gatgtattag atgtgagcga atttagccat
       61 tttagcttcg ctgaaacttc gttttcgtat caatcacagc acacattttg ctgaacgaca
      121 aaaccatatt aatggaattg agaacttttg gaaccaggca aaacgtcatt tacgcaagtt
      181 taacggcatt cccaaagcgc attttgagct gtatttaaag gagtgcgaat ggcgttttaa
      241 caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaacc gagtttatcc
      301 tagttatcta ggacagcccc ttattttttg ttcggcggct tgcgtggtcg ggtaaaatga
      361 aagttttgaa cggttggtcg gacaggaaga tgtggcgggt tttgagtgct ttgccgatag
      421 gcgtggtgtt ttttgatttg atctacggtt ttgtgttgaa tgtgttgcag ggtttggatt
      481 tgcagcgtgc cgtgccggat tcggaaggcg tgttggcggt tacgcccgat attgcattca
      541 acagtttgca gattgtcgcc aacggcggta tggcggcggt ggtctgtttc gggttggcgg
      601 ttgtgttttt gctcaaccgt tcggtgcggc ggcggcaggt gttggaaatc ggggtgttcc
      661 ggatgttggg gctggtggcg gtattggcgt tcagcgcgcc gtcggtgtgg gagtgggcga
      721 acgcgctgcc gctgctgctg aagggcgcgg acgtggtcaa tacggggaat gcgcgttatg
      781 tgctgacggc tttgtgtatg ccctttcctg cggtgtcgtg cgtcatcggg ctggtggggc
      841 ggttcaggct tcagacggca tcgggcaggg cggcaaagtc agggggtgcg ggcaaggcgg
      901 acggatagga cgcatttttc agcgggtgcg tcgagaagca gccgatgtgt ttggcagccg
      961 cagcttgggg ggtgtagtgc taatggcggt ttctttgctt ttatagtgga ttaacaaaaa
     1021 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     1081 caagtgaatc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
     1141 ttgttaatcc actatataaa ataaatgggc aaaaatcggt ttattatcgt ttttgccgca
     1201 tttggatttg ttctaccgta aaacgtgttt gacgaacggg attcttatta aaaaacatct
     1261 gatttctaac aaaatcagta ttttttggca cgatggctaa aatttttcct tccatttcgc
     1321 catcacgtgt tttccatgcg ctcaagaatt gtgatttgct cattgagacg tgccccagcg
     1381 atggatcagc cagcaaaaca gtttctccgt taataccgtt caataccgaa aaatggttgt
     1441 ttttacggta ttttaaatac acaattacag gaatttttag ttgtaccaac tgttcaaatg
     1501 gcaaagcata accttgtgct tcaaaaccca gttcgggcat tatgcgttgc atatcatcaa
     1561 aagaagcacg catttgggtt ttatccattt tgtctaagat ttccgcttca gaataatgtc
     1621 tgccataaaa attattcagt aacgtggcaa tcgaagccgc gccgcaagaa aaatccaaat
     1681 cttgttttac tatgccggaa tctcgccgtg ctttccaact ccgtacatgg atgttttggt
     1741 aagaagcggg gggtcaacaa acataggcca agcaaaaact atatttgggg cgaaaccaat
     1801 caaagccgca taatttatca atttataaag attttttatc ataatatgta tacgcggaat
     1861 aaacgcatga aacataaaaa aataaaatca tatgcaattt tattgcatga ataaatatga
     1921 ataaaataga taatgatggg agtaaatacg ccatgtattt tggaagttta aatttattaa
     1981 taataaaata atttatcgta gcgcaaataa atcccaaaat tggaacgatt agaaaaaaaa
     2041 ttaatacacc catcatcatg caaactctat attaataaat agctaactaa ttctattgtg
     2101 gaaattagtt agctaactaa aagttattaa tgattatttt cgagaattga ctgcattgtt
     2161 ggcagcattg gcaccaaaac ctagtgcatg aatacccggt ctccatgcca aattcccagc
     2221 caatccgcct ccggcagcag cagccagccc tgttttgccg ctactcctgt tgccgcaccg
     2281 attcctgccg cagtagccgc gccttgcgca gttcctaatt taccatgatt atacaaatta
     2341 gcaccatgat actcccatgc acctaatgca ccgccaaaag cagcggctgc aataatggga
     2401 acaaattcac cttgtgtttc tttcatttca gcctgtgata attgaattgc tttcacattt
     2461 tggctgtcaa aaacttggct gtctaaattt tgcgccatta caggtgtaat catcatagcc
     2521 attacagttg caattttcgt tgcgctggtt tgcacataaa taggattagc aaattcgctt
     2581 tgattgcgtt cagtgttgat gtagctaata ctgctttcta gtttgaattt acccttgtca
     2641 gtcaataatt cttccaaact taaaggtaag tcagcataag caatttggct ggcaacagtt
     2701 aaaataaaat ctattagaca tttgtgtttt tgcatcattt cgtttgattt tctagttttt
     2761 gagaatgata caaagttttt tacaaagtaa agagtcactc tgaaaaacat tttttcatta
     2821 taaatcaaaa tattgataga ataaatagcg agcatcgatt cacggtgcac tttagtgcaa
     2881 agcgtacaac gcgagcctga accaccagcc gcaacaggaa aagaaagccg atagagtgca
     2941 atgcttgcca acgtgcaagc gagcttgcaa gaacgcttgg ctcaacgaga gcaggcaaga
     3001 cagaaagcag aaagcaggat aggagcggta acgcaaaggt ctcgggcttt gatttcgccg
     3061 taaaccctgc tgccgccttg tccggaaagg gtgcaggcgg cgagtgccga cagggtgcag
     3121 atggggaggg gggttttcat ttggggtcgc aacggaagtg gtatgcgcag atttcaaaac
     3181 cgtttttgaa atacaggcgg tgcgcgtcgg cacggtcgtg gttgacgtgg acgttgaggt
     3241 ggattttggt tacccctgtt tccgcgccga ttttgcggac ttcttccaaa aggcgcgagg
     3301 cgtagccttt gcggcggctt tgcggcaggg taacgatgtc atcgatgtgg atgtggcggc
     3361 cgctggcgag ggtgcaggct tcgcggaagc cgcagacggc gacggcattg tgtttgcctt
     3421 cttcaaaaat acccagcagg cggtagcctt gggggcgttg gactttgttg atctgttcgg
     3481 taaagcggtt gatgtcggtc agggcggaac gcaaaacgct caaggctgca aaggcggtgg
     3541 cggtgtcgtc cgcgccgatt tcgcgcaaaa cgtaggatgc gcccgaggcg gtctgttcct
     3601 gtgctttctc ggcggcgtgt ttttcttcga ttgcctgtgt cagcatgacg tgttcgtcgg
     3661 cagggttgtt ttgtccgccc tgttcgcgtt cttcgagcag ggctttgcag tcgatgacgc
     3721 gcaggtcgtt gtcggcggca aagtccatca ggaagcggaa catttgggga ttgtcggttt
     3781 ccagtttttt atcgacggcg acgcagcgga tgctgtccac cagtatgggg cgcacccatt
     3841 tcgaatagga aagcctgtgg tctttggtga acgaggacag aatgccgcac aggcgttccg
     3901 cccagtcgct gggacggaaa atcttgccgg aactcgttgt gccgtggatg acgacttcgt
     3961 aggggttgca gactaacatg gcggcttcct gaaaagaaat gtctggctcg attatacctt
     4021 atgcttatgc aggcgtgttt ggatatgccg tctgaaaagt acgggattcg tgcggtaaaa
     4081 ctttgcggcg gcaaatgtgc gataatacgc gccgtattgc cgcttttgcg aagctgttcc
     4141 gcaaacatac gggcggcgtg gacgacgtat aaccggatac ccgcctgacg cgggtttttt
     4201 acggaagggg ggcaaaaatg cctaatccgc tttacagaca gcatatcatc tccatttcgg
     4261 atttgtcgcg cgaacagttg gaatgcctgc ttcagacggc attgaagctg aaggcgcatc
     4321 cgcgcggcga tttgttggaa ggcaaactta tcggttcgtg cttctttgaa ccgtccacgc
     4381 gcacgaggct gtcgtttgaa acggcggtgc agcggctggg cggcaaggtc atcggctttt
     4441 cggacggcgc gaataccagt gccaaaaaag gcgagacgct tgccgatacc gcccgcatca
     4501 tttccggcta caccgacgca atcatccaac gccaccccaa agacggcgcg gcgcgcgtgg
     4561 cagcggagtt ttcgcgcgtc cccgtcatca acgccggcga cggcaccaac cagcacccca
     4621 gccagacctt gttggatctg gtaacgattt atgaaacgca ggggcgtttg gacaagctca
     4681 aaatcgccat ggcgggcgac ttgaaatacg ggcgcaccgt gcattcgctt tgtcaggcgt
     4741 tgaaacgctg gggctgcgaa tttgcctttg tttcgccgcc cagcctcgcg atgcccgact
     4801 acattaccga agagttggac gaagccggct gccgataccg tatcctcggc agtttggaag
     4861 aggcggcgga atgggcggac atcctgtata tgacccgcgt ccagcgcgaa cgtttcgatg
     4921 aacaggaatt tgccaaaatc caaggcaaat tcaacctcga agcgtctatg ctcgcccacg
     4981 ccaaaccgaa cctgcgcgtg ctgcaccctt tgccgcgcgt ggacgaaatc catcccgatg
     5041 tcgatgccac gccgcatgcc tattatttcg agcaggcaac caacggcgtt tatgcgcgta
     5101 tggcgatatt gtcgctggtg ttgaacgaag aagtgtgagg aaccgatatg gaaaccccga
     5161 aactcagtgt cgaagccatt gaaaaaggta cggttatcga ccatattccc gccggcaggg
     5221 ggctgaccat cctgcgccag ttcaaacttt tgcactacgg caacgcagta accgtgggct
     5281 tcaacctgcc cagcaaaacc caaggcagca aagacatcat caaaatcaaa ggcgtgtgct
     5341 tggacgacaa agccgccgac cgccttgccc tgtttgcacc cgaagcagtg gtcaacacca
     5401 tcgacaattt caaggttgtg cagaaacggc atttgaacct gcccgacgaa atcgccgaag
     5461 tgttccgctg tccgaacacg aattgcgccg gccacggcga gccggtcaaa agccggtttt
     5521 atgttaaaaa acacaacggg cagacgcggc tgaaatgcca ttactgcgaa aaaacctaca
     5581 gccgggattc ggtggcggaa gcctgacgga ttcccttaaa ccgagcgggc ggcatttcgt
     5641 ctgccgcttg ttttgccaat ctgaaatgga atgatgatgc acgcttctgt ccaaagccgt
     5701 ttcgcaccga tactttatgt tttgattttc tttgccggtt ttttgaccgc gcaaatctgg
     5761 ttcaatcaga aagcctatac tgaagagctg cctccgcttc tgtccgcatt gtccgccgtc
     5821 gcgctggtgt ggctggcgtg ggcgttcgtg tcggcgcgtt caaaggctaa ggcggaaaag
     5881 ttctaccgcg aaaaaatgat acagaacgaa agcatacacc ccgtcctgca cgcttctttg
     5941 caacacttgg aacacaagcc gcaaatgctc gccctgctgg tcaaaaacca cggcaaaggt
     6001 atggcggagc aggtcaggtt caaggcagaa gtgctgcccg acgacgaaga cgcgcgcacg
     6061 attgccgccg agttggcaaa aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg
     6121 ggcgaaacct atggacgcgt gttcgccgat attttcgagt tgtcggcggc tttggaaggg
     6181 cgcgcgttca aaggaatgtt gaaactgacg gcggaatata aaaacatctt cggcgatgcc
     6241 tgccgttcgg aaacggcgtt ggagttgggc gcgctcaatc aggcgttgca ggagatttca
     6301 aaaacatcgg aaaagtccaa acggatattt tattgaagat ggaaaaatgc cgtctgaaac
     6361 ggaaggtgtt tcagacggca tttttgtcgg atgattaatt attcggagcg gttgaagcca
     6421 aacttcacgc ggctgcggcc ctgatccggt atattgtcca aatcgcgtcc cggattggcg
     6481 gcggtgtcgc ctacggaaat atcggagatg ttttccaaaa tgatggcgga cgacaggtgt
     6541 tcggaggtgc ggtaaaccat tgccaagccc acttcttcgg caggagtgga aatcagctcg
     6601 acggtatccc tgcttttgaa attgttggag aggtcgacct gcatcgtttt cttgcgtttg
     6661 tagaggctca aaaccgtgcc tttgtccaaa ccgtccgcct cgcctttgtc gatggtgatg
     6721 gtttgaaact ggccggcaat ccttgtgcct tcaaacacgg aaacgatttt agcctgaacc
     6781 gggcgggacg gttcgtgcgg catcatgttg aagcggtcgg tgtcttccgg cattttcatc
     6841 aggtagtcgc cctgctgtat ttcggaaatg gcggtttcga ccaccagcgg ctgtatcgaa
     6901 ggggtgcgca gcggggtaat caaaggatgg gtgcgggtat ggtattcgtt gtctttcggc
     6961 cgttctccag cctgtttcga gcgttgttcg aggacagagt cggtatagtc gagggagcgc
     7021 acgatgccgc tgaatgcgac ttcctgcccg aggaatttac ccgtatccgg atcggtgatg
     7081 tttttattga ttcggtaggt caggtagcga cccggctctt tcaggccttt ggtgtaaacc
     7141 ctggtgcctt tggtgtacag cagcctgcct tccgggcccg agagcaggcg cggcgcggca
     7201 gcggtttctt tgcgggaaac gatttgcggg tgccgcataa agatgcggta gaagttgaca
     7261 tcgatggcgg gaataccgta tccggacact tccttatccg gactcatttt gacgacgggg
     7321 atgccgtctg tctgttccaa gccgaggcgc ggttcgccgt caacgtggcg caacaccaat
     7381 acctggtccg gataaatcag gtcgggattg tggatttgat cccggttcgc gtcccacagg
     7441 cggccccatt gccacgggct gtacaggtat ttgcccgaaa tgccccacag ggtgtcgccc
     7501 tgtttgaccg tgtagcgttc cggcgcgttc gggcgcacct ccaaatttgc cgccaaagtt
     7561 tgtgttgaga atgccatacc tgccgcgcag agcagggtta taatacgacg ttgcataacc
     7621 gttcccctta tctgataaat ttcggtttgt cttgcttgat tgggttggaa aaagcggcgg
     7681 cagcccctcg ggatgtgccg cgtgataaaa aatgttccgc attttaacat cgaattatcc
     7741 gcaccatcac ggtaattatg aaaaacaggc ggcgtatccg ccgaaggaaa gagaaaatta
     7801 tggctttatt gaatatcttg caatatcccg acgagcgtct gcacacggtg gcaaagcctg
     7861 tcgaacaagt cgacgagcgc atccggaagc tgattgccga tatgtttgaa acgatgtacg
     7921 aatcgcgcgg catcgggctg gcggcgacgc aggtcgatgt gcacgagcgc gtggtcgtga
     7981 tggatttgac cgaagaccgc agcgaaccgc gcgtgttcat caaccccgtc atcgttgaaa
     8041 aagacggcga aaccacttac gaagagggct gcctgtccgt gccgggcatt tacgacaccg
     8101 taacccgcgc cgaacgcgtc aaggtcgagg ctttgaacga aaaaggcgaa aagttcacgc
     8161 tggaggcgga cggcttgctg gcgatttgcg tgcagcacga gttggaccac ctgatgggca
     8221 tcgtgtttgt cgaacgcctg tcgcagctca agcagggacg catcaaaacc aagctgaaaa
     8281 aacgtcagaa acatacgatt tgaccctttt gccgtgccgt ctgaacgctg caaagttttc
     8341 agacggcacg gtcttgtccg acaaccttac gcatacgcag gaatatgcta tgaaagtcat
     8401 ctttgccggc acgcccgatt ttgccgccgc cgccttaaaa gccgttgccg ccgccggttt
     8461 tgaaatcccg ctggtgctga cccagcccga ccgtccgaaa gggcgcggta tgcaactgac
     8521 cgccccgccc gtcaaacaag ccgcgctgga actcggtttg cgcgtggcac agcccgaaaa
     8581 actgcgtaac aacgccgaag ccctgcaaat gctcaaagag gtcgaggcag acgtaatggt
     8641 ggttgccgcc tacggtttga ttctgccgca ggaagtgttg gatacgccga aacacggctg
     8701 cctcaacatc cacgcttcgc tgttaccccg ttggcgtggc gcggcgccga ttcaacgcgc
     8761 gattgaagcc ggcgatgccg agacaggcgt gtgtattatg cagatggaca tcggtttgga
     8821 caccggcgat gtggtcagcg aacaccgcta cgccatccaa ccgaccgata ccgccaacga
     8881 agtccacgac gcgctgatgg aaatcggtgc ggcggcggtt gttgccgatt tgcaacagct
     8941 tcaaagcaaa ggccgtctga acgcggtcaa acagcccgaa gaaggtgtta cttacgccca
     9001 aaaattgcgc aaagaagagg cgcgtatcga ttggagcgaa agcgcggcgg ttatcgaacg
     9061 caaaatccgc gccttcaacc ccgtgcctgc cgcgtgggtt gagtatcagg gcaagccgat
     9121 gaaaatccgg cgggcggaag tggtggcgca acaaggcgcg gcaggcgaag tgttgtcctg
     9181 ttcggcggac ggtttggtcg ttgcctgcgg cgaaaacgcg ctgaagatta ccgaattgca
     9241 gcctgccggc ggcaggcgta tgaatatcgc ggcgtttgca gcaggacggc atatcgaagc
     9301 agggacgaag ctgtaaacct ttttcagacg gcatttgatc cgcaaacggg aatgccgcct
     9361 gaaaccatca gttgaagaaa gcgaatcaca taatatgagt atggcacttg cccaaaaact
     9421 tgccgccgac agcattgcgg cggttgccga aggacgcaac cttcaggacg tgttggcgca
     9481 aatccgcacc gcgcatcccg accttacggc gcaggaaaac ggcgcgttgc aggacatcgc
     9541 ctacggctgc cagcgttatt tgggcagttt gaaacatatg ctcgcgcaga tgctgaaaaa
     9601 gccgattgac aacccgcagc tcgaaagcct gcttttggcg gcgttgtacc aactgcatta
     9661 cacgcgcaac gcgccgcacg ccgtggtcaa tgaggcggtg gaaagcatcg cgaaaatcgg
     9721 gcgcgggcag taccgttcgt ttgccaacgc ggttttgcgc cgttttttgc gtgaacgtga
     9781 aaaattggcg tcttcctgca aaaaagacga tgtggcaaaa cacaacctgc cgctgtggtg
     9841 ggtggcttac ttgaaaaacc attatccgaa acactggcac aacatcaccg ccgcgctgca
     9901 atcgcatccg ccgatgacgt tgcgcgtcaa ccgccgacac ggcaatgccg aaagctattt
     9961 ggaaaaactg gctgcggaag gtatcgccgc aaaggcgttg gacgaatatg cggttacgtt
    10021 ggaagaagcc gtgccggtaa accgtctgcc cggtttttca gacggcattg tttcggtaca
    10081 ggacttcggt gcgcagcagg cggcgtatct ccttaacccg aaagacggag aacggatttt
    10141 ggacgcgtgc gccgcgccgg gcggcaagac ggggcatatt ttggaactgg cggattgccg
    10201 cgttaccgcc ttggacatcg atgcaggccg tctgaagcgt gtggaggaca atatcgcccg
    10261 tttgggcttt cagacggcat cggcggcgtg tgccgatgcg cgggatttgg cggcgtggta
    10321 tgatggaaag cctttcgacg caatccttgc cgacgtgccg tgcaccgctt ccggcgtggc
    10381 gcggcgcaat cccgacgtga aatggctgcg ccgtccgacc gacgcgctaa aaaccgcccg
    10441 ccagcaggaa gccctgctgg acgcactttg gcagacgctg aaacagggcg gcagaatgct
    10501 gcttgccacc tgttccgtgt tcgtcgaaga aaacgacggg caattgcaaa aattcctcaa
    10561 ccgccatgcc gatgccgaac cgatcgaatc gcgggtgctt ttaccgaaca aacaccaaga
    10621 tggcttttat tacgcgctta ttcaaaagca ttaaacaatg gcttgtgctg ctgccgatgc
    10681 tttccgtttt gccggacgcg gcggcggagg ggatagatgt gagccgcgcc gaagcgagga
    10741 taaccgacgg cgggcagctt tccatcagca gccgcttcca aaccgagctg cccgaccagc
    10801 tccaacaggc gttgcgccgg ggcgtgccgc tcaactttac cttaagctgg cagctttccg
    10861 ccccgataat cgcttcttat cggtttaaat tggggcaact gattggcgat gacgacaata
    10921 ttgactacaa actgagtttc catccgctga ccaaccgcta ccgcgttacc gtcggcgcgt
    10981 tttcgacaga ctacgacacc ttggatgcgg cattgcgcgc gaccggcgcg gttgccaact
    11041 ggaaagtcct gaacaaaggc gcgctgtctg gtgcggaagc aggggaaacc aaggcggaaa
    11101 tccgcctgac gctgtccact tcaaaactgc ccaagccttt tcaaatcaat gcattgactt
    11161 ctcaaaactg gcatttggat tcgggttgga aacctctaaa catcatcggg aacaaataat
    11221 gcgccgtttt ctaccgatcg cagccatatg cgccgtcgtc ctgttgtacg gactgacggc
    11281 ggcaaccggc agcaccagtt cgctggcgga ttatttctgg tggattgttg cgttcagcgc
    11341 aatgctgctg ctggtgttgt ccgccgtttt ggcacgttat gtcatattgc tgttgaaaga
    11401 caggcgcgac ggcgtattcg gttcgcagat tgccaaacgc ctttctggga tgtttacgct
    11461 ggttgccgta ctgcccggcg tgtttctgtt cggcgtttcc gcacagttta tcaacggcac
    11521 gattaattcg tggttcggca acgataccca cgaggcgctt gaacgcagcc tcaatttgag
    11581 caagtccgca ttgaatttgg cggcagacaa cgccctcggc aacgccgtcc ccgtgcagat
    11641 agacctcatc ggcgcggctt ccctgcccgg ggatatgggc agggtgctgg aacattacgc
    11701 caacgggttt acacagttgt ccctttacaa tgccgcaagc ggcaaaatcg aaaaaagcat
    11761 caacccgcac aagctcgatc agccgtttcc ggataaggcg cgttgggaaa aaatccaaca
    11821 ggcgggttcg gtcagggatt tggaaagcat aggcggcgta ttgtacgcgc agggctggct
    11881 gtcggcgggt acgcacaacg ggcgcgatta cgccttgttt ttccgtcagc cggttcccaa
    11941 aggcgtggca gaggatgccg tcttaatcga aaaggcaagg gcgaaatatg ctgagttgag
    12001 ttacagcaaa aaaggtttgc agaccttttt cctggcaacc ctgctgattg cctcgctgct
    12061 gtcgattttt cttgcactgg tgatggcgct gtatttcgcc cgccgtttcg tcgaacccgt
    12121 cctatcgctt gccgaggggg cgaaggcggt ggcgcaaggc gatttcagcc agacgcgccc
    12181 cgtgttgcgc aacgacgagt tcggacgctt gaccaagttg ttcaaccaca tgaccgagca
    12241 gctttccatc gccaaagaag cagacgagcg caaccgccgg cgcgaggaag ccgccaggca
    12301 ttatcttgaa tgcgtgttgg atgggctgac cacgggcgtg gtggtgtttg acgaacaagg
    12361 ctgtctgaaa accttcaaca aagcggcgga acagattttg gggatgccgc tcgcccccct
    12421 gtggggcagc agccggcacg gttggcacgg cgtttcggcg cagcagtccc tgcttgccga
    12481 agtgtttgcc gccatcggcg cggcggcagg tacggacaaa ccggtccatg tgaaatatgc
    12541 cgcgccggac gatgccaaaa tcctgctggg caaggcaacc gtcctgcccg aagacaacgg
    12601 caacggcgtg gtgatggtga ttgacgacat caccgtgctg atacacgcgc aaaaagaagc
    12661 cgcgtggggc gaagtggcga agcggctggc acacgaaatc cgcaatccgc tcacgcccat
    12721 ccagctttcc gccgaacggc tggcgtggaa attgggcggg aagctggatg agcaggatgc
    12781 gcaaatcctg acgcgttcga ccgacaccat cgtcaaacag gtggcggcat tgaaggaaat
    12841 ggtcgaagca ttccgcaatt atgcgcgttc cccttcgctc aaattggaaa atcaggattt
    12901 gaacgcctta atcggcgatg tgttggcatt gtatgaagcc ggtccgtgcc ggtttgcggc
    12961 ggagcttgcc ggcgaaccgc tgatggtggc ggcggatacg accgccatgc ggcaggtgct
    13021 gcacaatatt ttcaaaaatg ccgccgaagc ggcggaagaa gccgatgtac ccgaagtcag
    13081 ggtaaaatcg gaagcggggc aggacggtcg gattgtcctg acggtttgcg acaacggcaa
    13141 agggttcggc agggaaatgc tgcacaacgc cttcgagccg tatgtaacgg acaaaccggc
    13201 gggaacggga ttgggtctgc ctgtggtgaa aaaaatcatt gaagaacacg gcggccgcat
    13261 cagtttgagc aatcaggatg cgggtggcgc gtgtgtcaga atcatcttgc caaaaacggt
    13321 agaaacttat gcgtagcagc gatattttaa ttgtagacga cgaaatcggc atccgcgacc
    13381 tgctgtcgga aatcctgcag gacgaaggtt attcggtcgc attggcggaa aacgccgaag
    13441 aggcgcgcaa gctgcgccat caggcgcgcc ccgcgatggt gctgctggat atttggatgc
    13501 ctgattgcga cggcatcacc cttttgaagg agtgggcgaa aaacgggcag ctcaatatgc
    13561 cggtggtgat gatgagcggg catgccagca tcgataccgc cgtggaagcc accaaaatcg
    13621 gtgcgctcga ctttttggaa aaaccgattt ccctgcaaaa gctgctgtcc acggttgaaa
    13681 acgcgttgaa gtacggtgcg gcgcaaaccg aaacggggcc tgtattcgac aagctgggca
    13741 acagtgcggc gattcaggaa atgaaccgtg aggtaggggc tgcggtgaaa cgtgcctctc
    13801 ccgtactttt gacgggcgag gcaggttcgc cgtttgaaac ggtggcacgc tatttccata
    13861 aaaacggtac gccgtgggtc agcccggcaa gggtcgaata tctgatcgat atgccgatgg
    13921 aactgttgca gaaggcggag ggcggcgttt tgtatgtcgg cgacatagcc caatacagcc
    13981 gcaacatcca agccggcatt gcctttattg tcggcaaggc agaacgctgc cgcgtcaggg
    14041 tggtcgcatc gggcagcagg gcggcaggtt cagacggcat tgcctgcgag gaaaagctgg
    14101 cggaactgct gtcggaatcg gtcgtccgta ttccgccgct gcgtatgcag catgaagata
    14161 ttcccttcct gatacagggg attgcctgca atgtggcgga aagccaaaag attgcgcccg
    14221 ccgcattcag tgaagatgcg cttgccgtgc tgacccgtta cgactggccg ggaaatttcg
    14281 accaactgca aagcgtcgtt gcaacgctgt tgttggaggc ggacggacag gaaatcggcg
    14341 cggcggcggt ttcttccctt ttggggcaga atgtgcctgc cgagggagca gaagatatgg
    14401 tgggcgggtt taatttcaac ctgcccctgc gcgaattgag ggaggaggtg gagcggcgtt
    14461 atttcgagta ccacatcgcc caagaaggtc agaatatgag caaagtggcg cagaaagtcg
    14521 gtttggaacg cacgcacctt taccgcaaac tcaaacagct cggcatcggc gtttcgcgcc
    14581 gggcggggga aaaaaccgaa gaataggccc ggacggccgg ttttccggct gcgggctttt
    14641 gttttcagac ggcatttggt gcaaatgccg tctgaaatcg taaggggacg gattttatga
    14701 cagaggacga acgtttcgcg tggctgcaat tggcgtttac gccctatatc ggcgcggaaa
    14761 gtttcctgct gctgatgcgc cgtttcggca gcgcgcaaaa tgccctgtcc gcaccggcgg
    14821 aacaggtggc ggcactgata cggcacaaac aggcgcttga ggcttggcgc aatgcggaaa
    14881 aacgcgctct ggcgcggcag gcggcagaag cggcattgga atgggaaatg cgggacggat
    14941 gccgtctgat gctgcttcag gatgaagatt ttcccgaaat gctgacgcag gggctgaccg
    15001 cgccaccggt tttgtttttg cgcggcaacg tacaactgct gcacaaacct tccgccgcca
    15061 ttgtcggcag ccgtcatgcc acgccgcagg caatgcggat tgccaaagat ttcggcaagt
    15121 cgttgggcgg gcaaaatatc cccgttgtgt cgggtatggc ttccggtatc gatactgccg
    15181 cccatcaggg cgcactggag gcagaaggcg gcaccatcgc cgtgtggggg acgggtatag
    15241 accgcattta cccgccgtcc aataaaaacc ttgcctatga aatcgccgaa aaaggattga
    15301 ttgtcagcga gtttcccatc ggtacgcgcc cgtatgccgg caattttccg cgccgcaacc
    15361 gcctgattgc tgccctgtcg caagtaacgc tggtggttga agccgccttg gaatccggtt
    15421 cgctgattac cgccggcttg gcggcagaaa tggggcgcga agtgatggcg gtacccggtt
    15481 cgatagacaa tccgcacagc aaaggctgcc acaaactgat taaagacggc gcaaaattgg
    15541 tggaatgcct ggacgacatc ctgaacgaat gcccggggct attgcaaaat acgggtgctt
    15601 catcatattc tataaataag gatacgcccg acacgggccg ccgcgccgtt cagacggcat
    15661 acgccccgcc gcctgccgca aaaatgccgt ctgaaggggc ggcaggcggc acagccgtcg
    15721 gcggcatact ggataaaatg ggtttcgacc ccatccatcc cgacgtgctt gccggacagt
    15781 tggctatgcc tgccgcagat ttgtatgccg cactgttgga attggaattg gacggcagcg
    15841 ttgccgcaat gcccggcggc agataccagc gtatccgaac ttgaacgcac tttatattaa
    15901 ggaacacgaa tgaccgaagt catcgcctac ctcatcgaac atttccaaga tttcgatacc
    15961 tgtccgccgc ccgaagactt gggtatgctg cttgaagaag cgggtttcga tacgatggaa
    16021 atcggcaaca ccctgatgat gatggaagta ttgctcaaca gctccgaatt ttccgccgaa
    16081 cctgccgaca gcggcgcatt gcgcgtgtac agcaaagaag aaaccgacaa cctgccgcag
    16141 gaagtgatgg ggctgatgca gtatctgatt gaagaaaaag ccgtcagctg cgaacagcgg
    16201 gaaatcatca tccacgcgct gatgcacatc cccggcgacg aaattaccgt agataccgcc
    16261 aaagtgctga ccctgctgct tttatgggca aacaagagcg agctgcccgt gttggtcggc
    16321 gacgagctga tgagcgcgct tttactcgac aacaaaccca cgatgaactg aagcggcttc
    16381 agacggcccg cccgaggccg tctgaaacgc cggcatcaaa accaccatcc agagaacgac
    16441 aaatggcgaa aaacctatta atcgtcgaat ccccgtccaa agccaaaacc ctgaaaaaat
    16501 atttgggcgg cgattttgaa atccttgcat cctacggaca cgtccgcgac ctcgtcccca
    16561 aaagcggcgc ggtcgatccc gacaacggct ttgcgatgaa ataccaactc atcagccgca
    16621 acggcaaaca cgtcgatgcc atcgtcgccg gtgccaaaga agctgaaaac atctacctcg
    16681 ccaccgaccc ggatagggaa ggcgaagcca tttcctggca tcttttggaa atcctcaaat
    16741 ccaaacgcgg cttgaaaaac atcaagccgc agcgtgtcgt gttccacgaa atcaccaaaa
    16801 acgccgtgct cgatgccgtt gcccatccgc gcgaaatcga aatggacttg gtcgatgcac
    16861 aacaagcccg ccgtgctttg gactatttgg tcggctttaa cctttcgcca ttgttatgga
    16921 aaaaaatccg ccgaggtttg agcgcggggc gtgtgcaaag ccctgctttg cgcctgattt
    16981 gtgagcgcga aaacgaaatc cgcgcgtttg aagcgcagga atattggacg gtgcatctcg
    17041 acagccacaa aggccgcagc aagttcaccg ccaaactcgc ccaatacaac ggcgcgaaac
    17101 tcgaacaatt cgacctgtcg aacgaagccg cgcaagccga cgtattgaaa gaactcgaag
    17161 gcaaagaagc cgtcgttacc gccattgaaa agaaaaagcg cagccgcaat cctgccgcgc
    17221 cgttcaccac ttctaccatg cagcaggatg ccgtgcgcaa actcggcttc accaccgacc
    17281 gcaccatgcg taccgcccaa cagctttatg aaggtataga cgtagggcag ggcgcgatcg
    17341 gtctgattac ctatatgcgt accgacagcg tgaatttggc ggatgaagcg ttaaccgaaa
    17401 tccgccatta cattgaaaac aaaatcggca aagaatatct gccgagtgcc gccaaacaat
    17461 acaaaaccaa atccaaaaac gcccaagaag cccacgaagc catccgtccg acttccgtgt
    17521 accgcacgcc cgaaagcgtc aaacccttcc tgagcgcaga ccagttcaaa ctctatcaaa
    17581 tgatttggca gcgtaccgtc gcctgtcaga tgacgcctgc caaattcgac caaaccaccg
    17641 tcgatattac cgtcggcaaa ggcgtattcc gcgtaaccgg acaagtgcaa accttcgcag
    17701 gcttcttgag cgtttatgaa gaaagcagcg acgatgaaga aggcgaagac agcaaaaaac
    17761 tgcccgaaat gagcgaaggc gataaattgc ccgtggacaa actctacggc gaacaacact
    17821 ttaccactcc gccgtcgcgc tacaacgaag ccacgctggt taaagccctc gaagaatacg
    17881 gcatcggccg cccctcgacc tacgccagca tcatctccac gctcaaagac cgcgaatacg
    17941 ttacccttga gcaaaaacgc ttcatgccca ccgacacagg cgacatcgtc aacaaattcc
    18001 tgaccgaaca cttcgcccaa tacgtcgatt accacttcac cgccaaactc gaagaccagc
    18061 ttgacgaaat tgccgacggc aaacgccaat ggattccctt gatggacaaa ttctggaaac
    18121 cgttcatcaa acaagtggaa gaaaaagaag gcatcgaacg cgccaaattt accacgcagg
    18181 aacttgatga aacctgcccg aaatgcggcg aacacaaact gcaaatcaaa ttcggcaaaa
    18241 tgggtcgttt tgttgcgtgt gccggttatc ccgagtgcag ctacacgcgc aatgtcaacg
    18301 aaaccgccga agaggccgcc gagcgcatcg ccaaagccga agccgaacag gccgaactcg
    18361 acggacgcga atgtcctaaa tgtggtggtc gcctagtgta caaatacagc cgaaccggca
    18421 gcaaattcat cggctgcgcc aactatccga aatgcaaaca cgtcgagccg ctggaaaaac
    18481 cgaaagatac cggcgtccag tgtccgcaat gcaaaaaagg caacctcgtc gagcgcaaat
    18541 cccgctacgg caaactgttt tacagttgca gcacctatcc cgactgtaac tacgccacat
    18601 ggaacccacc cgttgcagaa agctgtccaa aatgcgcatg gccggtttta accatcaaaa
    18661 ccaccaaacg ctggggcgtc gaaaaagtct gcccgcaaaa agaatgcggc tggaaagaac
    18721 agattgaacc gcctgcgccg aaagagtgag attaggttgg tttgaaagag aaaaggtcgt
    18781 ctgaaaaatt ttcagacgac ctttgctttt ctgtgattgg tttatttgaa tccgcgtgtt
    18841 gttttaaagt ccgataaaat ccggttcatt tcaggcgcaa acaaggcgat gtaatcgtaa
    18901 gatagaccgc gactggcact gggatgggga aagcagacga cttcgcaatc ttcaaacgat
    18961 tggaatttga cattgaaacg tgtaccgtca aattcttttt gcaccgtctc cagcggtttg
    19021 gtctgcttac cgaccaactg ctcgaagcgt ggcagtacat tttggttgtt cagaaaatcc
    19081 gccaacctgc tgcccatgaa gaggatgact ttcggacgca gtttttcgat gtggtagaga
    19141 aaattatcga tgtgctcggg ttgtgtgaac ttgtcgggat tgtcgatagt gttgccctgt
    19201 gtagcagccc agttggtttg aaccagggat ttttcaaatg caccgcccaa tccattttcg
    19261 tctaaggggt gtccccacat ttcaaaccaa ttttttatcg tattgtcgta acgccacttt
    19321 tttgcctgct ctccgaaata gagggatttg tttgcaaatg tatggtcgat tttgttttca
    19381 gggagtttgt attcacctgc tacataagca gcctcatcgg ctttactcca accccattca
    19441 tagccacaaa tcattaagcc atgtttgtcg ttgtagcctt tgaacaggct gttgctcaaa
    19501 ttcaaatcct tcatcatgaa ctcttccttt taaaatttaa gagcgattga cttcaatgtt
    19561 tttagatggg gtggaaaaat ccttgtgtag gcaacataaa ttcaataaat ttcttgataa
    19621 ttcgaaacct actaatagcg cacctataaa agctttttca ttacgttcag catgacggtc
    19681 acgtcgttca tattttttac gcttgctgtt ccctgttatt acagctaagc caagtgatat
    19741 ggcgagaatt gcccaaacaa tagtacttaa taacaatttt ccccatacta tcaataagga
    19801 aagaaaaaac cttttagtat tagatcgata ggttataatc catgcccatg aaaatgcaat
    19861 aagagttata cataagatac aagctgacag gatttcttta tttttaatta aataacttag
    19921 agcgatgagg atgacaggtg tcaagaaaat aatagttaca tcccgatagc tataaaagaa
    19981 aactgcccta ttttgaatgt ggagatgtgc acagaatcca atatagctaa ggataatagt
    20041 taatataata aaaaaagacc accaagggtg aagagatagg aattccatgt tttccctttt
    20101 tttgtaaaaa ggaaaaaatc ttatctaata gttaattgcc taaccagcca gaagaagttt
    20161 aaaatctatc ccaataattc aaccatctat acagaaagtt cagcttatgg aaacccacga
    20221 aaaaatccgc ctgatgcgcg aattgaataa atggtcccag gaggatatgg cggaaaagct
    20281 ggcgatgtcg gcaggagggt atgccaaaat cgaacggggc gaaacgcagt tgaatatccc
    20341 gcgtttggag cagttggctc agattttcaa aatcgatatg tgggacttgc tcaaatcggg
    20401 cggtggtggg atggtgtttc agattaatga aggtgatagt ggtggcgata tttgcgttgt
    20461 atgcgtcggg tgatgtttcg atgaaaatag aatttttaaa aatggagttg aaacactgca
    20521 aagaaatgtt ggaacaaaaa gacaaagaaa tcgagctgct ccgcaagctg accgaaaccg
    20581 tttaaacaga tatgccgtct gaaaaaagtt ttcagacggc atattctttg acaggtcttg
    20641 tataataccg tttgaactta caggtttttg attatggcgg caggcaaaca taccaaacac
    20701 agcaaccggg tacgcattat cggcgggcaa tgccggggca ggaaattgag tttcacatcc
    20761 gccgacggac tgcgtccgac acccgacagc gtgcgtgaaa agctgtttaa ctggctggga
    20821 caggatttga cgggtaaaac ggttttggat ctcttcggag gcagcggcgc actcggtata
    20881 gaagccgctt cgcgcaacgc caaacgcgtg ctgatttcgg ataacaaccg ccaaaccgtg
    20941 cagaccttgc agaaaaacag tcgtgaactg ggtttggggc aggtgcaaat cgtctgttca
    21001 gacggcatcg catatttgaa gaccgtatcc gaacagtttg atgttgtctt tctcgacccg
    21061 ccgtttgcat ggcaggactg gcaaatcctg tttgatgcct tgaagccgtg cctgaacccc
    21121 cgggcattcg tctatctcga ggcgggtacg ctgccggata ttcccgattg gctgacggaa
    21181 tatagagagg ggaaatcggg gcagagtaca tttgaattaa gggttttcca agtggctgaa
    21241 taatatgcgc tttgataatc atttccgagt tgtaaacatt cgtttgcaac cgtccggttc
    21301 aaaaaaacct tgtgctataa tccgcgcccg cccggttttg ataatttagt ggaaaaggaa
    21361 aagaaatgtc gctttttatt accgacgagt gcatcaactg cgacgtatgc gaacccgaat
    21421 gccccaatga tgccatttcc caaggcgagg aaatttacga aatcaacccc aacctctgca
    21481 cgcagtgcgt cggacactac gatgagccgc agtgccagca ggtttgcccg gtggactgca
    21541 tcctgattga cgaagaatat cccgaaaccc atgacgagtt gatggcgaaa tacgaaaaga
    21601 ttatccagtt taaataaatt ctttttaaaa catcaaatta tgtctgtttt gaaataaaat
    21661 caaaaaaaaa cttgacggaa aagcaagccg ctaataaact aacgttctct tttggagggg
    21721 ttcccgagcg gtcaaagggg gcagactgta aatctgttgc gaaagcttcg aaggttcgaa
    21781 tccttctccc tccaccaaaa ttcttacttg gggcagtagc gagtaatgcg ggtgtagctc
    21841 aatggtagag cagaagcctt ccaagcttac ggtgagggtt cgattccctt cacccgctcc
    21901 aaacaattag gcccatgtag ctcaggggta gagcactccc ttggtaaggg agaggccggc
    21961 agttcaaatc tgcccatggg caccatctct cgattattca tttctttaag gcttagatat
    22021 ataggatatt g
//
LOCUS       DAWFIT010000027        21905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFIT010000027 DAWFIT010000000
VERSION     DAWFIT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.027"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(131..1087)
                     /gene="gshB"
                     /locus_tag="WGQ85_000978"
     CDS             complement(131..1087)
                     /gene="gshB"
                     /locus_tag="WGQ85_000978"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0750422.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1180..2868)
                     /locus_tag="WGQ85_000979"
     CDS             complement(1180..2868)
                     /locus_tag="WGQ85_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0750423.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(2980..3753)
                     /locus_tag="WGQ85_000980"
     CDS             complement(2980..3753)
                     /locus_tag="WGQ85_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0750424.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3796..4698)
                     /locus_tag="WGQ85_000981"
     CDS             complement(3796..4698)
                     /locus_tag="WGQ85_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0750425.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            complement(4847..5563)
                     /locus_tag="WGQ85_000982"
     CDS             complement(4847..5563)
                     /locus_tag="WGQ85_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0750426.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6132..6554)
                     /locus_tag="WGQ85_000983"
     CDS             complement(6132..6554)
                     /locus_tag="WGQ85_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0750427.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6672..7343)
                     /locus_tag="WGQ85_000984"
     CDS             complement(6672..7343)
                     /locus_tag="WGQ85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0750428.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(7359..7985)
                     /locus_tag="WGQ85_000985"
     CDS             complement(7359..7985)
                     /locus_tag="WGQ85_000985"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0750429.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8092..8910)
                     /locus_tag="WGQ85_000986"
     CDS             complement(8092..8910)
                     /locus_tag="WGQ85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0750430.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9169..9609)
                     /locus_tag="WGQ85_000987"
     CDS             complement(9169..9609)
                     /locus_tag="WGQ85_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0750431.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(9672..11078)
                     /locus_tag="WGQ85_000988"
     CDS             complement(9672..11078)
                     /locus_tag="WGQ85_000988"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0750432.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFAEAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            11248..12363
                     /gene="lptF"
                     /locus_tag="WGQ85_000989"
     CDS             11248..12363
                     /gene="lptF"
                     /locus_tag="WGQ85_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0750433.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLDLRNGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            12360..13430
                     /gene="lptG"
                     /locus_tag="WGQ85_000990"
     CDS             12360..13430
                     /gene="lptG"
                     /locus_tag="WGQ85_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0750434.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGLIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13740..16325
                     /gene="acnB"
                     /locus_tag="WGQ85_000991"
     CDS             13740..16325
                     /gene="acnB"
                     /locus_tag="WGQ85_000991"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0750435.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16467..17462
                     /locus_tag="WGQ85_000992"
     CDS             16467..17462
                     /locus_tag="WGQ85_000992"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0750436.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(17531..17632)
                     /locus_tag="WGQ85_000993"
                     /pseudo
     CDS             complement(17531..17632)
                     /locus_tag="WGQ85_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17686..18699)
                     /gene="ilvC"
                     /locus_tag="WGQ85_000994"
     CDS             complement(17686..18699)
                     /gene="ilvC"
                     /locus_tag="WGQ85_000994"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0750437.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(18779..19072)
                     /locus_tag="WGQ85_000995"
     CDS             complement(18779..19072)
                     /locus_tag="WGQ85_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0750438.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(19139..19630)
                     /gene="ilvN"
                     /locus_tag="WGQ85_000996"
     CDS             complement(19139..19630)
                     /gene="ilvN"
                     /locus_tag="WGQ85_000996"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0750439.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(19641..21368)
                     /gene="ilvB"
                     /locus_tag="WGQ85_000997"
     CDS             complement(19641..21368)
                     /gene="ilvB"
                     /locus_tag="WGQ85_000997"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0750440.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         5062 a         5978 c         5657 g         5208 t
ORIGIN      
        1 ataccgtctg aaaacggggc ggcggggtgt ccgtacggca ttaaccgcat ccctgccggc
       61 tgagggaaaa ccctgcctgc ccgaacaaac caaacggttg tgaagcaaaa gcctttcaaa
      121 cggcatcggt ttaacgtacc gaccacgcgg caacggcatc ggcaaacatt gccgccacgt
      181 cgaaaccttt ttgtttcata atttcttgga atccggtcgg gctggttacg ttgacttcgg
      241 tcaggttgct gccgataacg tccaaaccgg ccagcaggat gccgcgccgt ttgagttcgg
      301 gggcgagcgt ttcggcaatt tcgcggtcgc gtccgtccaa ttcctgcgcc acgccgcgtc
      361 cgcctgccgc aaggttgccg cgtgtttcgc cgttttgcgg gatacgcgcc aaagcatagg
      421 ggacgacttc gccgccgata atcaggatgc gtttgtcacc gtgtacgatt tcgggaatgt
      481 agcgttgcgc cataatggtg cgggaatcaa gccgcatcag ggtttcgagg atgctgccga
      541 tgttggggtc tttttcggtc aggcggaaaa tacccatacc gcccataccg tccagcggtt
      601 tgatgataat gtcgccgtgt tctttcaaaa atgcgcggac atcggcggaa cgggtcgtta
      661 ccagcgtggg cgcggtaaag cggctgaagt tcaaaatcgc cagtttttca ttgaagtcac
      721 gcatcgactg tccgctgtta aagactttcg cgccctgctg ttccgccagc gtcagtaatt
      781 gggtggcgta gaggtattgc atatcgaacg gcggatcggt acgcataatc acggcatcaa
      841 atgctttcaa tgccgtctga actttgtcgg cggcggcaaa ccagtttttg tcgtggtcgt
      901 cttgtgcgcc gagaaactca aaagcagacg catcagcaac caccagaccg tttttaaccg
      961 acaattcccc gctcaaggta tgaaacagcc gccaaccgcg ttttgccatc tcgcgcatca
     1021 tggcgtaggt ggtgtctttg taggttttga aacttgccat cgggtcggcg ataaagagga
     1081 ctttcatcat atttcctttc cggtgtgccg aatgtgccgc atttcgcggg caaaggggaa
     1141 atgccgtccg aacaatattc agacggcaag gatggggttt tacttaggct gccaagaatc
     1201 tttcagcgtg accgtgcggt taaacaccgg cgtgtctttg ccgtggtctt tacggtcggt
     1261 tacgaagtag ccgatacgct cgaactgcca acggctttct gccggcaaat ctttggcggc
     1321 aggttcggcg taggcggtga tttccttgac ggattccgga ttgaggaaat cggtgaacgg
     1381 caggtattcg ccgtcttcgc cgcgcacggc atcgggacgc tcgatggtaa agaggcggtc
     1441 gtacagacgg actttgattt cggcggcgtg ttcggcggaa acccaatgaa tcacgccttt
     1501 aactttacgg ccttctggat ttttgcccaa ggtgtcgtgg tcgatgctgc atttgagttc
     1561 aaccacattg cctgcttcgt ctttgacgac ttcatcgcac ttgatgacat agccgtggcg
     1621 caagcgtact tcgccgccgg gaatcaggcg tttgaagcct ttgggcggat tttcggcaaa
     1681 gtcgtcggct tcaatataga tggtttgtga aataggtact tcgcgctcgc ccatttcctc
     1741 gtggttcgga tggaacgcgg cacggcggct ttgggttctg ccggcttcaa agttggtcag
     1801 ggtcactttg agcgggttca acaccgccat caggcgtggg gcggaatttt ccaactcttc
     1861 gcgaatcgcg ccttccaaca cgctcatatc gacgatgttt tcagatttgg aaataccggc
     1921 gcgtttggca aacagacgca ggccttcggg cgtgtagccg cgtcggcgca taccggaaat
     1981 agtcggcata cgcggatcgt cccagccgga aacgtgtttt tccacaacca actgattcaa
     2041 tttccgtttg gaggtaatgg tgtacaaaag ctccaaacgg gaaaactcgt attggcgcgg
     2101 acgggtggca tgcggcgcgt ggatgttatc caacacccag tcgtacagcg gacggtgtgc
     2161 ttcaaattcg agcgtacaca aggaatgcgt gatgccttcg atggcatcgg agatgcaatg
     2221 cgtgtagtcg tacatcgggt agatacacca tttgtcgccg gtgttgtggt gatgggcgcg
     2281 gcggatgcgg tagatgacgg ggtcgcgcat attgatgttg cccgatgcca tgtcgatttt
     2341 caggcgcagg gttttgctgc cgtcggggaa ctcgccgttt ttcatgcgtg tgaacaggtc
     2401 gaggttttct tcgacgctgc ggtcgcggta agggctgttt ttacccgctt cggtcagcgt
     2461 accgcggtat tcgcgcattt cttcgggcgt caaatcatcg acatacgctt tgccgtcttt
     2521 aatcaaaccg acggcgtagt cataaagctg gtcgaaatag ttggaagcga aacgcggctc
     2581 gcccgcccaa tggaaaccga gccactcgac atcttctttg atggcgttga cgtattcgtc
     2641 gttttctttt tcggggttgg tatcgtcaaa acgcaggttg cacaagccgt cgtaaatata
     2701 cgccaaaccg aagttcaggc agatggattt ggcgtgtccg atgtgcaggt agccgttggg
     2761 ctccggcgga aaacgggttt ggacggcttc gtgtttgccg cttttgaggt cgtcttcgat
     2821 gatggtgcgg ataaaatggt tgtccgcaaa ttggtcttta ttgagcatag ttttctttga
     2881 acagatggct tcagacggca ttggggcggt tccttatgcc gtctgaagcg gtttgatgat
     2941 gtgtttattg tacccgactt gcgcgctttg acatagcgtt cagacggcat cggcaatcag
     3001 gcattccacc cccgcctctt tcagcatctt ctgcatcgcg gtatcgggca gccggtcggt
     3061 aaatactttg tcaaacgccg taatgtcgcc gagcctgacc agcgcgttgc tgcggaattt
     3121 actgtggtcc acgccgagga agcggacgcg cgcgttggca atcatcgcct gcatcacgct
     3181 gacttctttg tagtcgtcgt ccaaaagcga accgtcgctt tccacgccgt gcgtactcat
     3241 cacggcataa tcgactttga actggttgat aaaatcgacg gttgccacgc cggtaatgcc
     3301 gccgtccaaa gggcggacga ctccggaagt gatgatgacc gtgtaatccg tccgcgccga
     3361 aacgatggag gcgacgtgga tattgttggt aataatccgc aggctgccgc gccgtctcac
     3421 cagctccgat gccacggctt ccatcgtcgt gccgatactg acaaacagcg atgactcgtc
     3481 gggaatatgt tccgcaatca gccgggcaat ggcgtttttt tcgttttgac attgaatttg
     3541 gcggctggca ggcagcccct ccggcaagtt tccgcccgag gatgcgccgc cgtggtggcg
     3601 tttcaggctg ccagcatcct ccaattcgcg gatgtcgcgg cgtatcgtct gcggggtaac
     3661 gtccaatgcg gcggcaagct cgtccaccga cataaactga tgccggcgga caaggcttaa
     3721 aatctctccg tgcctttgga ttttcggttt catcgttttc tgcctccttg catcgggatg
     3781 ccgattttac cgcgttcagc ccaaagcgga aaacaccacc atcagaaacg gagcggcgat
     3841 attgaccacc acgccgaagc tgaccgctac cggtacggct tccaagccgc ccgcaccccg
     3901 aatcacgggc aatgtgaaat ccatactggt cgcgccgccg acccccactg ccgcatcggg
     3961 aaaacgcttc atcagcagcg gaataaatgc cagcgcgaac agctctcgtg ccaaatcgtt
     4021 caaaagcgcg atactgcccc ataccgcgcc gtaagcctcg gtcatcacca aacccgagag
     4081 ggaataccaa ccgaagccgg aagccatcgc caaacctttc acccacgaca caccgtctgc
     4141 cgatgcggca aacagcagcc cgcctgaaag agatgaaagc ataaaccaga ccgacaaccg
     4201 aatacccctg cggttgacca aaacctgccg caacgatacg ccgctgcttt tgagctgtac
     4261 gccgatgagg agcaccagca gcatcagaca atacataccc gcgttttcag acggcatcca
     4321 aatatcgcgc atcagtttgc cggatgcaaa tccgagcagc acgcatccga gctgccccac
     4381 actgcccgac acaccgaccg aaacgccctt ccctttcccc tttatccgcc acgggaataa
     4441 ctttcccaac actgccaaag caagcaggtt cgccccgacc gtacaaacaa acagccacag
     4501 aaccgtcaac gccatatcgt ccaaccgcga acccaaatcc tccacgcgcg acaacgagac
     4561 gccgatcagc agcagcacag catacaccaa gaccgatagc accttatcca aagcgggcag
     4621 gtaaggcttg ggcacacgga taaaaaatcc ggcaaacatc ggtatcaata ccgaaagcaa
     4681 cgtcatcagg ctgtccatct cctgacctcc tttattgccg catgatatat gcggtttaaa
     4741 aattgccgtc tgaaaattgc agatacccgc atccatattt cagacggcat caggttcgcc
     4801 attaaaaaac cgcctgaagg ttcaggcggc ttatccgctc cggcattcaa tcttccaaag
     4861 tcttttccaa acgctccata cagttgccca aatggcggcg caggattttg accacgcggt
     4921 tgcgcctgcc cgccagcagc aggtcgagga tttcgcggtg ttcggaatgc gtatgcgtat
     4981 tgatggcgtg tttttcctcg cgatgcacgc ccgccacggc gacaatcagg gaagaccgcg
     5041 cgcacagcgt gttcataatg tcgaacagca catcgttgcc caccaggcgc gccagttcga
     5101 cgtgaaaggc attggacagg cggttccagc caacgcggtc tcccctgccg gaggcctctt
     5161 cttcgcgccg tatcatcgca taaagcggct tgaggcgcgt ttccaaatcc ggcaaatctg
     5221 cgaggatatt caaaatcatc gtctccattt cgatgcgcgc attgaacaca tcctgcattt
     5281 ctttcaaatc gggaacgtgg acgaacgcgc ccctgttggg ttgcaaatcg acaatcttgt
     5341 cgtgcgccaa aagcgacagc gcgccgcgga cggtgttgcg cgaacacacc atctggcggc
     5401 aaagttcgga ttcggtcagc tttttgccag gcagcagcac ctgatcggta atgccgtcca
     5461 aaatcagggc gtaaacacgg aacagctccg aatcgtgccg ctcttcgaga atcagggaag
     5521 acgtggtcgg cgcatggata atgtcgtcgt tttcaaagtt catgatgttt tccgtatttt
     5581 tacgctttca aatttcttaa gatgttttaa ggcggctgtg tttcaaatcg tgtcggggaa
     5641 attaaagcat tgcacaaatt tattttatag tggattaaca aaaatcagga caaggcgacg
     5701 aagccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag caccttagag
     5761 aatcgttctc tttgagccaa ggcgaggcaa cgccgtactg gtttttgtta atccactata
     5821 attcaataaa ttaatatatg gcttaaaata acgggattct cgcctcccgc ccgcccgcag
     5881 aagcaggcgg gtatcatttt aaaacgcggc atttaaaatt tgaccgaaaa ttgttgacaa
     5941 tccggaatca agtctgcaca ataccccgac aagtccaagt attataaagg ctgaataaag
     6001 aggaaacagc aggcagatat attcgggagg tgcagtccga atatatctgc ttttttatgc
     6061 gcctccggat tgcctgccgc acctttccct tcagacggca tcagccgttt ccccataatg
     6121 ccgcccgatg cctatttatc tgccccggca atttcaaaac tgtgggtaat ctttgccgct
     6181 ttgcccaaca taatcgaagc cgaacagtat ttttcggcag acatctgaac ggcgcgctca
     6241 atggccgatt ctttcaaatc atgcccgaat actttgaaat ggatgtggat ttcggtaaac
     6301 acgcgcggcg catcgtccgc ccgtttcgcc gtaaccgtcg cacggcagtc agtcactttc
     6361 tgacgctgtt tttcggcaat catcaccaca tcgatgctcg aacagcccgc cacgcccaac
     6421 agcagcattt ccaaagggct gggcccgcgc ttggccgcac cttctgccgc cgacccctcc
     6481 ataacgacgc tgtgcccgcc ttccgttgtt ccgacaaagc acattccgtc tatccatttt
     6541 gatgtaacct gcatggtgtc attcctgaaa atagcgttaa aaccgctttg catatggcgt
     6601 tattgtaaac aatttcaagc ggcttatgca gaaatatgga caaaacggca aaaaaacgcc
     6661 tgaaaatcga tttacggttt ggctgcctgg ccgttgatct gcaccgattt gagtttcagc
     6721 gtataggttt tgccgtcgtc ggtatagccg atttgtgccg gaatattgtt cagggacggt
     6781 gcgaagaaat acattaccgc atcgtcgccg cgccgcaccc gatatttgac gacttcggtt
     6841 tccacgccgc ctatgctgta ttttcctgta cccgccttat tcaaaccgcc gacggaataa
     6901 agttttttgc cgttggtgat tttcagcccc ggggggagtt tcgcgtcatt tgccgccaac
     6961 tgccaggcaa gcgtgaacaa atccatagcc ttggggcttt gctcggtttt gctctcgccc
     7021 gctttgccgt aggttacgct gccgtcggcg aatttggctt ccgcatacag tttgcccctg
     7081 cgtatgtctc tatagtaggt agggtgcagg gtattgccga caaccgtacc gccggactcg
     7141 aaacggatat tgtatagcgg cactttaatc gtcgaaacga ttttgtaagc attgccgctg
     7201 cgttcaaatg tcatcgtggc gggaatgccg tagctgccgg aatagtgcag cacggcggat
     7261 tggggcagcc ctgccgcata cgcgcacggc agggcggcgg acaaaatggc ggcggaaaat
     7321 atatttttaa aagtcttcat catttgctcc cgcccggttt acgccgtcag gaaacgggcg
     7381 gcatcggcgt tttccgaatt tctgacgcgg tttccctcga taatcaggcg gccggcggca
     7441 aaatcggcaa cggctttcgg ataaagttta tgctcaacag ccaaaacccg ggcggcaaca
     7501 tcgtctgccg tatcgccgtc gagtatcggc acaatccctt gcgatacaat cgggccgcaa
     7561 tccagttcgg cagtaacgaa atggatggtg cagccggcaa cgcggcagcc cgcctccaaa
     7621 gcgcgttcgt gcgtatgaag tccggtaaac gagggcagga cggacgggtg aatgttcatc
     7681 agcctgcctt cgtaatgggc acagaactca ggggtcagaa tccgcataaa acctgccaaa
     7741 accaccaagt cgggttgata tgcgtcgatt ttttccatca tggcggtatc gaaggcaagt
     7801 cgtgattcaa agtttttatg attcaggctg tcggtcggaa taccctgacc ggccgcccat
     7861 tgcaaaccgg cagccgtttc gctgttgctc aacacggcgg caatacggac gttgggaatg
     7921 gcggcattga cgattgcctg catattgctg ccgcgtccgg aaatcaggat gacgatgttt
     7981 ttcataatgg tgcgcttttg aaagggatgc catctgaacc gctgtttggt ggtttcagac
     8041 ggcatttgcc gtaaaaatgc ccgaaaacct gtttcgggca tggattcgga cttaatttac
     8101 ttttttgatg tcgacttgag ccggctgctt ggcgggcgcg ttttcgggtg cgccgatttt
     8161 gaccagtttc acatcaaata ccaaagtggc gttcgggccg attttgtcgc ccgcaccctg
     8221 ttcgcggtag gcaaggttgg acgggatgta gaacgtggct tcgccgcctt ctttcagaag
     8281 ctgtacgcct tcggtccaac ccggaatcac ttggctcaaa gggaaggtga ccgggccgcc
     8341 gttggctttg ctgctgtcga ataccgtacc gtcaatcagg cggccttcgt attccacggt
     8401 aacgatgtcg tctttggtcg gctgtttgcc ttcgccctgt ttggtgattt tgtattgcag
     8461 gccggaagca gtggtcttca cgccgtcttt ggcggcattt tctttcagaa aggcttcgcc
     8521 tttttcttta ttggccttcg cgtccgcctt gtgtttttct acggctttag cctgttgttc
     8581 ctgaaggaat ttcatcatga cttcctgagc ctgctcttcg gtcattttga tttctttgcc
     8641 gtcatacact gcctgcatgg cttcggtaaa gactttcaaa tcgatttccg cgccctgttc
     8701 cttcatttgc ttcagggagc gtccgatgtc cacgcccatc gcatagcttg cctgctgcat
     8761 cgtgctgccg atcgaagagg tgtcgccctg cgcggaagaa gcggcggcag gttcggatgc
     8821 agatgcgggg gcggcttctt ttttgccgca ggcggaaagt gccaaagcgg cggaaagggt
     8881 cagtgcgctg attttgaaaa tggtgttcat gatggatctt cgctgtcgat aaggtcggaa
     8941 aaacgggatt atagccgagt ttgaatgttt caacacacag gatgacacat aaagcgtcaa
     9001 tcgtgtgttg ccctgttttg gaaaagattg aaccttccaa aataagtttt gattctaccg
     9061 ccccgaggga cagatgtcca agtggcgggg ttcaaccgat aaggaaattt taatcaaata
     9121 gaatcaagcc tgtttaaatt ttgtaaatgc ggcatttcag acggcatttt atgccttgcc
     9181 ctccatgccg tgatgttcga tggcaaaacc gcttcggcgg taggcggtaa agcgttcgcg
     9241 cgcgtcggcg agctcttcca ggctgttgcc gacgatttct aaaacgcgcg cgggcaagac
     9301 cggagcggtg ttccaaaaac cgtcggacag gttcaaaacg gccatgcctt cgggaattcg
     9361 gggcagattg ccgccgcagg caagcaggac ggatggttca gacggcatgg cttcttccgt
     9421 ttcccaaatt tcgtgcggaa taaaactctc ggcctcgtat tgccaaagca ttttgtccaa
     9481 ttcctgaagc tgcccgaacg aatcggacca caccagtatc ctgccgccgt cccgaatcgc
     9541 acgggcaatc aggcggcagg tgaaaatcgg aacttgggca acgtgcgtgt aaaaggtggc
     9601 tttcggcata ttgtttgagc atttggcagg acgatgccgt ctgaaaggct tcagacggca
     9661 ttgtggcaaa attaaagatt ccgcagatag ttcagcagca agggaacggg acggccagtc
     9721 gcaccttttt ccgctccgga tttccacgcc gtacccgcga tgtcaaggtg cgcccacggg
     9781 tagccttcgg tgaagtaaga caggaatgtt gcggcggtaa tcgtgcccgc gccgggcgtg
     9841 ccgatattgg ggatgtcggc aaagttggat ttgagctggt ctttgtaggt ttcaaagagc
     9901 ggcagttgcc acgctttgtc gtccacgttg taggaagcgg caagcaggct gtcgaccaaa
     9961 tcttgattgt tgcccatcac gccgctgaca tcgtgcccca aggcgacaat acacgcgccg
    10021 gtcagggtgg cgacgtcgat gacggctttg ggtttgaact gttcggcgta agtgagcgcg
    10081 tcgcacaaaa tcagacggcc ttcggcatcg gtgttcaaca cttcgatggt cagacctttc
    10141 atacttttca cgacatcgcc cggtttgttt gccgcgccgg aaggcatatt ttcacaagtg
    10201 gcgacgacgg caatcaggtt aatcggcagt tgcagtttga cggcggcgca gaaggtgctg
    10261 atgacggttg ccgcgccgca catatcaaac ttcatttcgt ccatgttcag gccgggtttg
    10321 agggaaatgc cgccggtgtc gaaggtaatg cctttgccga ccaataccac aggcgcggct
    10381 tctttgtccg ccgcaccgaa atagctcagt tcgaccaaat agggatcttc gacactgcct
    10441 ttggcgaccg accaaaacga acccatgttt tctttgatgt agtctttttc aatgattttg
    10501 gcgtgtgcgc ccaatttttc ggcttcggct ttggcggtgc gcgctaaaaa ttcgggcgtg
    10561 cattcgttgg gcgcggcgtt gcccaagtcg cggcagaggc tttgtccgta aacctgcgct
    10621 tcggcaacgc gcagggcttc tttgacggcg gcttcgtgtg cgctgtggaa cacggcttct
    10681 gcaaatttgg cgggcttggc ttctttttta tagcggtcga aacggtaggc ggcattgccg
    10741 aaagcaatcg caaacgcttc ggcaacggct gcggcttgcg cttcttcaaa ggcatgaacg
    10801 tccacattga ccgtttcctg attttgcgcc catttggcgg cttcggcagc ggccttgttc
    10861 aatgcggcgc ggccggtgct tttcagacag catacggcaa cagcctgcaa accgttgcct
    10921 gtcgggattt ttgtgtcggc aaaattttga ccttcttcaa gcgaagacaa aagggcaagg
    10981 gcggtcggat ggtttaattg cgcctcttcg gtgcagacaa ataactgtgc gcctgcctgc
    11041 tgttcctgca agatttcggt ttttgtgcta aattccacgt ttattctcct gattgagacg
    11101 gttgtcggta gttttcggac ggcctttcgc tcaaaagacc gtctgaagac ggctggcacg
    11161 attgtacccc atttgaagca ccgtctgaaa ccttgcgcgg acaatccgcc tgcgccgaac
    11221 cgcttaccgc cccctgaccg cgattctatg atttatcaaa gaaacctcat caaagaactc
    11281 tcttttaccg ccgtcggcat tttcgtcgtc ctcttggcgg tattggtctc cacgcaggca
    11341 atcaacctgc tcggccgtgc cgccgacggg cgtgtcgcca tcgatgccgt gttggcattg
    11401 gtcggcttct gggtcatcgg tatgacgccg cttttgctgg tgttgaccgc atttatcagt
    11461 acgttgaccg tgttgacccg ctactggcgc gacagcgaaa tgtcggtctg gctatcctgc
    11521 ggattggcat tgaaacaatg gatacgcccg gtgatgcagt ttgccgtgcc gtttgccgtt
    11581 ttggttgccg tcatgcagct ttgggtgata ccgtgggcag agctacgcag ccgcgaatac
    11641 gctgaaatcc tgaagcagaa gcaggaattg tctttggtgg aggcaggcgg gttcaacagc
    11701 ttgggcaagc gcaacggcag ggtttatttt gtcgaaacct tcgacactga atccggcatt
    11761 atgaaaaacc tgttcctgcg cgaacaggac aaaaacggcg gcgacaacat tatcttcgcc
    11821 aaagaaggca atttctcgct gaacgacaac aaacgcacgc tcgacctgcg caacggctac
    11881 cgttacagcg gtacgcctgg acgcgccgac tacaatcagg tttccttcca aaaactcaac
    11941 ctgattatca gcaccacgcc caaactcatc gaccccgttt cccaccgccg taccattccg
    12001 accgcccaac tgattggcag cagcaacccg caacatcagg cggaattgat gtggcgcatc
    12061 tcgctgaccg tcagcgtcct cctactctgc ctgcttgccg tgccgctttc ctatttcaac
    12121 ccgcgcagcg gacataccta caatatcttg attgccatcg gtttgttttt aatttaccaa
    12181 aacgggctga ccctgctttt tgaagccgtg gaagacggca aaatccattt ttggctcgga
    12241 ctgctgccta tgcacatcat catgttcgtc atcgcaatcg tacttctgcg cgtccgcagc
    12301 atgcccagcc agcccttctg gcaggctgtt ggcaaaagtc tgacattgaa aggcggaaaa
    12361 tgaacctgat ttcacgttac atcatccgtc aaatggcggt tatggcggtt tacgcgctcc
    12421 ttgccttcct cgctttgtac agcttttttg aaatcctgta cgaaaccggc aacctcggca
    12481 aaggcagtta cggcatatgg gaaatgctgg gctacaccgc cctcaaaatg cccgcccgcg
    12541 cctacgaact gatgcccctc gccgtcctta tcggcggact ggtctctctc agccagcttg
    12601 ccgccggcag cgaactgacc gtcatcaaag ccagcggcat gagcaccaaa aagctgctgt
    12661 tgattctgtc gcagttcggt ttgattttcg ccattgccac cgtcgcgctc ggcgaatggg
    12721 ttgcccccac gctgagccaa aaagccgaaa acatcaaagc cgccgctatt aacggcaaaa
    12781 tcagcaccgg caataccggc atttggctca aagaaaaaaa cagcattatc aacgtgcgcg
    12841 aaatgttgcc cgaccatacc ctgctgggca ttaaaatctg ggcccgcaac gataaaaacg
    12901 aactggcaga ggcagtggaa gccgattccg ccgttttgaa cagcgacggc agttggcagt
    12961 tgaaaaacat ccgccgcagc acgcttggcg aagacaaagt cgaggtctct attgcggctg
    13021 aagaaaactg gccgatttcc gtcaaacgca acctgatgga cgtattgctc gtcaaacccg
    13081 accaaatgtc cgtcggcgaa ctgaccacct acatccgcca cctccaaaac aacagccaaa
    13141 acacccgaat ctacgccatc gcatggtggc gcaaattggt ttaccccgcc gcagcctggg
    13201 tgatggcgct cgtcgccttt gcctttaccc cgcaaaccac ccgccacggc aatatgggct
    13261 taaaactctt cggcggcatc tgtctcggat tgctgttcca ccttgccgga cggctcttcg
    13321 ggtttaccag ccaactctac ggcatcccgc ccttcctcgc cggcgcacta cctaccatag
    13381 ccttcgcctt gctcgccgtt tggctgatac gcaaacagga aaaacgctaa accaatgccg
    13441 tctgaccccc ttcggacggc attggttttt attatcatat tcctgtagac agatagtcgt
    13501 tccctattat agtggattaa caaaaaccag tacggcgttg cctcgccttg ccgtactatt
    13561 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat acattacctg
    13621 tcataacagt tccatttttg ttaaaactag tctatgatag cggtacaaat attgtttaca
    13681 atatttaacg caaatcattt gcaacccgac aaaagaaaaa cagaaaaagg aacaaagaga
    13741 tgttagaagc ctatcgtaaa gccgccgccg agcgcgccgc cctcggcatt cccgccctcc
    13801 ctttgaacgc gcagcaaacc gccgatttgg ttgagctgct gaaaagcccg cccgcaggcg
    13861 aaggcgagtt cttggtcgaa ctgcttgccc accgtgttcc gcccggtgtg gacgatgccg
    13921 ccaaagtcaa agcctcattc ctggctgccg ttgccgaagg cagcgcgtcc agcccgctga
    13981 tctcccccga atatgcgacc gaactcttag gtacgatgct cggcggttac aatatccacg
    14041 ccttaatcga actcttggac gacgacaaac tcgcgcccat tgccgccaaa ggcttgaaac
    14101 atacgcttct gatgttcgat tccttccacg acgttcagga aaaagccgaa aaaggcaaca
    14161 aatacgcgca agaagttttg caatcttggg cagatgccga atggtttacc tcacgcgcca
    14221 aagttcccga aaaaatcacc gttaccgttt tcaaagttga cggcgaaacc aatacagacg
    14281 acctctcccc cgcgcccgac gcgtggagtc gtcccgatat tccgctgcac gcgctggcca
    14341 tgctgaaaaa cccgcgcgac ggcatcacgc ccgacaaacc gggcgaagtc ggtccgatta
    14401 aattgttgga agaactcaaa gccaaaggcc atccggttgc ttacgtcggc gacgtggtcg
    14461 gtaccggttc ttcacgcaaa tccgcgacca actccgtcat ttggcatacc ggcgaagaca
    14521 ttccgttcgt gccgaacaaa cgcttcggcg gcgtatgttt gggcggcaaa atcgcgccga
    14581 ttttcttcaa tacccaagaa gattccggcg cgctgccgat tgaagtcgat gtatctgctc
    14641 taaaaatggg tgatgtcgtc gatatcctgc cttatgaagg caaaatcgtg aaaaacggcg
    14701 agactgttgc cgagtttgaa ttgaaatcac aagtattgct ggacgaagtg caagccggcg
    14761 gccgtatcaa cctgattatc ggccgaggtc tgaccgccaa agcgcgcgaa gccctgaaac
    14821 tgcctgcctc tactgcattc cgcctgccgc aagcgcctgc cgaaagcaaa gccggtttca
    14881 ccttggcgca aaaaatggtc ggccgcgcct gcggtctgcc cgaaggacaa ggcgtgcgcc
    14941 cgggtactta ctgcgaaccg cgtatgacga cggtcggctc gcaagacacg accggcccga
    15001 tgacccgcga cgagttgaaa gacttggctt gtttgggctt ctccgccgat atggtgatgc
    15061 agtctttctg ccacaccgcc gcctatccga aacctgtcga tgtaaaaacc cataaagaac
    15121 tgcccgcctt tatttccacc cgtggcggcg tgtcactgcg tccgggcgac ggcgtcatcc
    15181 actcgtggct caaccgcctg ctgctgcccg ataccgtcgg caccggcggc gacagccata
    15241 cccgtttccc catcggtatt tccttccccg ccggctccgg cttggttgcc tttgccgccg
    15301 caacgggcgt aatgccgctc gatatgcccg agtctgtatt ggtacgcttc agcggcaagc
    15361 tgcaaccggg cgtaaccctg cgcgatttgg tgaacgccat cccgctgtac gcaatcaaac
    15421 aaggtttgct gaccgttgcc aaagccggta agaaaaacat cttctccggc cgcatcctcg
    15481 aaatcgaagg cctgcctgat ttgaaagtgg aacaagcctt tgaattgacc gacgcatccg
    15541 ccgaacgctc cgccgccggc tgtaccgtga agctcaacaa agagccgatt atcgagtaca
    15601 tgaaatccaa cgtcgtgttg atgaaaaaca tgattgccaa cggctatcaa gacccgcgca
    15661 ctttggaacg ccgcatcaaa gctatggaaa aatggctggc aaatcccgag ttgctcgaag
    15721 cggataaaga tgccgaatac gccgccgtga ttgaaatcaa catggacgac atcaaagagc
    15781 cgattatcgc ctgcccgaac gacccggacg acgtgtgctt catgtccgaa cgctccggca
    15841 ccaaaatcga cgaagtattc atcggttcgt gtatgaccaa catcggccac ttccgcgccg
    15901 cctccaaact tttggaaggc aaggcagaca cccccgtccg cctgtggatt gcgccgccga
    15961 ccaaaatgga cgcgaaacaa ttgtccgacg aaggacacta cggcgtactc ggacgtgccg
    16021 gcgcgcgtat ggaaatgccg ggttgctcct tatgtatggg taatcaggcg caagtacgcg
    16081 aaggtgcgac cgttatgtcc acctccaccc gcaacttccc gaaccgtttg ggtaaaaaca
    16141 cctttgttta cctcggttcg gcggaattgg cagcgatttg ctccaaactg ggtaaaatcc
    16201 cgaccgttga agaatatcaa gccaatatcg gcatcatcaa cgaacagggc gataaaatct
    16261 accgctatat gaacttcaac gaaatcgaca gctacaacga agtagccgag accgtgaacg
    16321 tttaatcccc gtcatccgta tgaagtaagg gattgaccgc aatgccgtct gaacaacctt
    16381 cagacggcat tgcaacattc cgctaaccct tctttccgca aacgctgcaa atacggcgtt
    16441 cacgccccca cataaaggaa acgacagtga acctgaaaaa ccgccatttt ctgaaacttt
    16501 tagacttcac gccggaagaa atcaccgcct acctcgacct tgccgccgaa ttgaaagccg
    16561 ccaaaaaagc agggcgcgag attcagcgga tgaaagggaa aaacatcgcc ctgatttttg
    16621 aaaaaacctc tactcggacg cgctgcgcgt ttgaagtcgc cgcgcgcgat caaggcgcgg
    16681 gagtgactta tttagagccg tccgccagcc aaatcgggca taaggaaagc atcaaagaca
    16741 ccgcccgcgt gttgggcagg atgtacgatg ccatcgaata tcgcggtttc ggtcaggaaa
    16801 ctgtcgaaga attggcgaaa tacgcgggcg tacccgtgtt caacgggctg accaacgagt
    16861 tccatcccac acaaatgctt gccgacgcac tgactatgcg cgaacacagc ggcaaacctt
    16921 tgaaccaaac cgcgtttgcc tacgtcggcg acgcgcgtta caacatgggc aattccctgc
    16981 tgattttagg ggcaaaattg gggatggacg tgcgtatcgg cgcaccgcaa agcctgtggc
    17041 cgtctgaagg cattattgcc gccgcacacg ccgccgccaa agaaaccggc gcaaaaatta
    17101 ccctgaccga aaacgcgcat gaagccgtga agaatgttga ttttattcat accgatgtgt
    17161 gggtcagcat gggcgagccg aaagaagtct ggcaggaacg catcgatttg ctgaaagatt
    17221 accgcgttac gcccgaactg atggcggcat cgggcaatcc gcaagtcaaa ttcatgcact
    17281 gcctgcccgc cttccacaac cgcgaaacca aagtcggcga atggatttac gaaaccttcg
    17341 ggctgaacgg tgtggaagtt acagaagaag tattcgaaag ccccgccagc atcgtgttcg
    17401 atcaggcgga aaaccgtatg cacacgatta aagcggtaat ggtcgcggct ctgggcgact
    17461 gacagaactg tgcctgttta aattcatccg caacacagat accgtctgaa cacgatgttc
    17521 agacggtatc catatatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc
    17581 gtactatttg tactgtctgc agcttcgtcg ccttgtcctg atttttgtta atccactata
    17641 aaaaaactgc ctacacgatg tgtaggcagt cccgtttgaa aacaatcagt ttttgtcttg
    17701 gtcaaccaat ttgttggcag taatccaagg catcatggca cgcagttgtg cgccgacttt
    17761 ttcaacttgg tggtcggcat tcaaacggcg gcgggcagtc atagacgcgt agttgacgtt
    17821 accctcttgg ataaacattt ttgcgtattc gccggtttgg atgcgtttca gagcattgcg
    17881 catggcttct ttgctggaag cattgaccac ttcagggccg gtaacgtatt cgccgtactc
    17941 cgcattgttg gaaatggagt agttcatatt ggcaataccg ccttcgaaaa tcaggtcaac
    18001 aatcagtttc atttcgtgca gacattcgaa gtaagccatt tcaggcgcgt aaccggcttc
    18061 ggtcagggtt tcaaaacctg ctttgatcaa ctcgaccacg ccaccgcaca atacagcttg
    18121 ttcgccgaac agatcggttt cggtttcttc gcggaaagtg gtttcaatca caccgccttt
    18181 ggtgccgccg ttggcagccg cataagacag ggcgatgtct ttggccttgc cggaattgtc
    18241 ttggtaaacg gcaatcagag aaggcacgcc gccgccgcgt ttgtattcac tgcgtacggt
    18301 atgacccggg cctttggggg caaccataat cacgtccaag tcggcacgcg gaacgatttg
    18361 gttgtagtga acgttgaagc cgtgtgcaaa tgccagcgtt gcgccttctt tcagattggc
    18421 tgcaacttcg gcgtgataga cggcaggcat ggtttcgtca ggcagcagca gcataacgac
    18481 atcggcttct ttggtcgctt cagcaacagt tttgacgaca tggccggctg cttcggcttt
    18541 tttccaagaa gaaccttggc gcagaccaat caccacgttt acacccgaat ctttcaggtt
    18601 ggcagcatgg gcatggcctt gcgaaccgta accgatgatg gcaacggttt tgcctttgat
    18661 cagggacaga tcggcatctt tatcgtaata gacttgcatt tgatttcctt taaggtaaat
    18721 ggttgtcgaa gccttaaaat gttgagcggc ttcggacggg ttaaacagag tgtgccgctt
    18781 aagcggcaac ttcattcatc aatacgattt ccaacgcttc ggttttgccg tcgacggact
    18841 ggacgaaggc ttggaaatgc gcgctggcat tgtgttcgtc aatagctgct tgggatttcc
    18901 aattttccac gaaaacaaaa cggttcggtt tgccgatttc ctgatggaga tcatagctga
    18961 tgttgccctc ttccgcacgg ctggctttga ccagttcttt aaactgtgct gccagtgttt
    19021 ctgtgtattc cggtttaacg gtaaccagtg cgacaatttt aatgttcgac ataaatctct
    19081 cctgccgttc gtttttcaga cggcattcaa ataccgtgcc gtctgaaagg ttacggcgtt
    19141 aaattttcaa aatacgctca ccgcgaccga tgccggccgc gcctgtacgt acggtttcca
    19201 aaatttgggc gcgtccgacc gtttccaaaa aggaatccag cttgtctgtc gagccggtaa
    19261 tttcaatcgt atagctgcgg tcggttacgt cgatgatgct gccccggtag atttcggtca
    19321 agcgtaaaaa ttcgtcgcgg tctttgccgg cggcacggac ttttaccaac atcagttcgc
    19381 gttcgacaaa acggctttcg ttcaaatcga ccactttaat cacttcaatc aatttattga
    19441 gttgcttggt gatttgttcg ataacctgct cgtcgccgtg tgtaacgatg gtcatacggg
    19501 acagggtttt gtcttcggtc ggcgcaaccg ccaaagaatc gatattgtaa tcgcgtgcag
    19561 agaacaaacc gaccacgcgg ctcatcgcac ctgattcgtt ttcgatcaga acagataaga
    19621 tatgtcgcat ttgcctctcc ttacgccttt ccgtccgcac gcatatgcgg cggaagtacc
    19681 atttcgtcca aacctttgcc gttgccgacc atgggcatca cattctgttt ctggtcggtc
    19741 aggaagtcga taaacaccag cctgtctttt tggttcaatg cttccaacaa cgcaccttcc
    19801 acatcagact tcttgtccac gcggataccg atatggccgt atgcctcggc aagtttgacg
    19861 aaatcgggca aagaatcgaa ataggtttcc gactctcgtc cgccgtaata tatttcctgc
    19921 cactggcgta ccataccgag ataaccgttg ttcagcgtaa tgacgttaac cggaatccga
    19981 tattggaaac aggtggacag ctcttggatg ttcatctgga tcgagccgtc gccggtaata
    20041 cagaatacgt cttgatccgg ggcggcaagt tttgcaccaa tcgcataagg cagaccaacg
    20101 cccatcgtac ccaaaccgcc ggagttgagc cattggcgcg gacgttcgaa gggataatat
    20161 tgagccgcaa acatttgatg ctgccctaca tccgatgtga tgattgccga attgccggta
    20221 atctcggcaa gcttctgaat cacatattgt ggcttgataa tttcgctgcc gttgtcaaac
    20281 cacaagcaat cgcgggaacg ccattcctct atggttttcc accatttgcc caaagcatct
    20341 tcagacggca cggactcttg tttttgccac agcgcaacca tctcggacaa aacgtttttc
    20401 acgtcgccga caatcggaat gtccgccttc acgcgtttgg cgatgctgga aggatcgaca
    20461 tcgatatgga taaccttctt cgccttctcg aaaaatttgg acggtacgga aaccacacgg
    20521 tcgtcaaaac gcgcacctac ggcaagaacg acatccgcat tctgcatggc aaggtttgcc
    20581 tcgtaagtac cgtgcatacc gagcataccg aggaattggc ggtcgccgga aggataagcg
    20641 cccaagccca tcagcgtacc cgtgcacgga gcacccgtca ttcggacaaa tcgggtcagc
    20701 tcttcagaag cattacccaa caccacgccg ccgccaaaat agacgaccgg acgtttggca
    20761 gatgccaaca tctgcacggc ctttttaatc tgaccgatat gcccttgaac aaccggttga
    20821 tacgaacgga taaaaatgtc ttcctgagga tagctgaatt tcgccatcgc ctgcgtaaca
    20881 tctttcggga catcaaccac cacgggcccc ggtcggccgc ttgcggcaat ttggaacgcc
    20941 tttttaatgg tttccgccaa ctcattgatg tccgtaacca ggaaattgtg tttgacgcac
    21001 ggacgggtaa tacccaccgt atcaacttct tggaacgcat ccgtaccaat cagggaattg
    21061 cctacctgcc cgctgatgac caccatcgga atcgaatccg tataggcagt agcaataccg
    21121 gtcagtgcat tggtaacgcc cgggccggat gtaaccaatg ccacgcccac cttaccgctg
    21181 acgcgcgcat acgcatctgc cgcgtgtact gccgcctgct cgtgacgggt cagaatgtgc
    21241 ttgaatttat tgagttggaa aagggcatcg tagatttcga taaccgcacc gccgggataa
    21301 ccgaaaacgt actcgacacc ttcggctttg agactctgca ctatgatttg cgcgcctgat
    21361 agctgcataa cgacctcttt tatacggttt caaaccaata gggacaaacc gctttgccac
    21421 agcacctgta atgcaattcc accaagcagc gatttagggt acgcgcattg ggggaacacg
    21481 gcaacagacg gattatccaa tcaattggaa aggaacacag agtttgtgaa aaagagtaga
    21541 aacgataacg caaaccgaca gttcaatcaa gaaaaatctt tcatctttta attatttttt
    21601 gaaagcagag aaattattga ttgattttaa aagaataaaa tcaggagtac cttttttgaa
    21661 agatggaaat tgttgacagt ttgtgtagga ggggcagatg tgaaaaaccc ttcttcgata
    21721 tcaagaattg taaaatttac agggtttcat cccaataaag actcgggata ttgattgaac
    21781 ttgattttat ttttgatata tcaaaaatat tcccaaccat acttcctgaa aatggctcat
    21841 tgcaccggac tgtattggac ggcattaaca gaaccaagag ggctaacaac gacttaatat
    21901 attga
//
LOCUS       DAWFIT010000028        20964 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFIT010000028 DAWFIT010000000
VERSION     DAWFIT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.028"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(347..1489)
                     /locus_tag="WGQ85_000998"
     CDS             complement(347..1489)
                     /locus_tag="WGQ85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750441.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASVANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1793..2056
                     /gene="rpsT"
                     /locus_tag="WGQ85_000999"
     CDS             1793..2056
                     /gene="rpsT"
                     /locus_tag="WGQ85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0750442.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2216..3364
                     /locus_tag="WGQ85_001000"
     CDS             2216..3364
                     /locus_tag="WGQ85_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0750443.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3507..4208
                     /locus_tag="WGQ85_001001"
     CDS             3507..4208
                     /locus_tag="WGQ85_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0750444.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            4266..6074
                     /gene="aspS"
                     /locus_tag="WGQ85_001002"
     CDS             4266..6074
                     /gene="aspS"
                     /locus_tag="WGQ85_001002"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0750445.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            6225..6509
                     /locus_tag="WGQ85_001003"
     CDS             6225..6509
                     /locus_tag="WGQ85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0750446.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6676..8568
                     /gene="speA"
                     /locus_tag="WGQ85_001004"
     CDS             6676..8568
                     /gene="speA"
                     /locus_tag="WGQ85_001004"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0750447.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8667..9590
                     /gene="speB"
                     /locus_tag="WGQ85_001005"
     CDS             8667..9590
                     /gene="speB"
                     /locus_tag="WGQ85_001005"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0750448.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9788..11254
                     /locus_tag="WGQ85_001006"
     CDS             9788..11254
                     /locus_tag="WGQ85_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0750449.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11461..12558)
                     /locus_tag="WGQ85_001007"
     CDS             complement(11461..12558)
                     /locus_tag="WGQ85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0750450.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGKGNLRLGN"
     gene            12781..13923
                     /gene="bioF"
                     /locus_tag="WGQ85_001008"
     CDS             12781..13923
                     /gene="bioF"
                     /locus_tag="WGQ85_001008"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0750451.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNIYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRQEVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13933..14580
                     /locus_tag="WGQ85_001009"
     CDS             13933..14580
                     /locus_tag="WGQ85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0750452.1"
                     /translation="MKTKFYDCQGEHLILYFAGWGTPPDAVNHLILPTNHDLLICYDY
                     QDLRLDFDFSSYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLICWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            14568..15350
                     /gene="bioC"
                     /locus_tag="WGQ85_001010"
     CDS             14568..15350
                     /gene="bioC"
                     /locus_tag="WGQ85_001010"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0750453.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            15570..17006
                     /locus_tag="WGQ85_001011"
     CDS             15570..17006
                     /locus_tag="WGQ85_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0750454.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            17009..18106
                     /locus_tag="WGQ85_001012"
     CDS             17009..18106
                     /locus_tag="WGQ85_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0750455.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            18118..18654
                     /locus_tag="WGQ85_001013"
     CDS             18118..18654
                     /locus_tag="WGQ85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0750456.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            18656..19300
                     /locus_tag="WGQ85_001014"
     CDS             18656..19300
                     /locus_tag="WGQ85_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0750457.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            19323..19682
                     /locus_tag="WGQ85_001015"
     CDS             19323..19682
                     /locus_tag="WGQ85_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0750458.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19775..19850
                     /locus_tag="WGQ85_001016"
     tRNA            19775..19850
                     /locus_tag="WGQ85_001016"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19808..19810,aa:Asn,seq:gtt)
     gene            complement(20349..20789)
                     /locus_tag="WGQ85_001017"
     CDS             complement(20349..20789)
                     /locus_tag="WGQ85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750459.1"
                     /translation="MNLFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(20731..>20964)
                     /locus_tag="WGQ85_001018"
     CDS             complement(20731..>20964)
                     /locus_tag="WGQ85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0750460.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
BASE COUNT         5288 a         4885 c         5363 g         5428 t
ORIGIN      
        1 ccgtgcggac gcgttcggat tcacgactgc gtgggaatga cgggatttca gtttgcggat
       61 tgatttgaag ttgcaaaatc ccaacggatc ggattttcgc tttcgcgttt caaagttacg
      121 gcgttatcgg aaaaacagaa aaccaaagct gcgaggattt atttaaaaca accgaatttc
      181 aacgaaccgg attttcgcct gcgccaggat gacggcggaa ggttttttgt cttttctgac
      241 agatgtccgc aatctgacat cccgaccgtg ggagcgacgg ttctatttca atccaatata
      301 aaatgccgtc cgaacgatgt tcggacggca tttttaatgc ttcaaatcag ttggtgccga
      361 ctttgatttt ctgccacagg ctgacagaca gttttttcgc atccgtgctc atttgcggca
      421 tcacaaaacc gtctttcata tcctgctcgt tcgggaagat ggaacgggtg ttcaccagct
      481 cggcaggcat tttttcgcgc gccggtttgc tggcgggggc gaaggttacg gcgatgccgt
      541 ttttcgccgc gatttcgggg tcgagcgtgt agttgatgta tttgtgggca ttggcgacgt
      601 ttttcgcatc ggcgggaatc agccaagact caatccagaa gcccatacct ttcggtgtca
      661 gcacttcgat gccgacgttg tttttcactt cttcggaacg tgctttcgcc aagttcaaat
      721 cgccgccgtt gcctgccgcc aggcagatgt cgccgcgcgc cagctcgtca atgatggacg
      781 ggctgaaacg tttgacatcc ggacggatag acttcaacac ttccgccgcc gccttcaaat
      841 cttcaggatt cgatcctttg ggatctttgc ccaagtagtt cagcaaaatc gggaacattt
      901 cgctcggggt gtcccacagg gcgatgccgc aggatttcag tttgtgggtg tattcaggtt
      961 tgaacagcaa atcccagccg ttttcgggca gcttgccgcc caaaagctct ttgcctttcg
     1021 ccgtaatcgc aatcgtgttc acgccggaga aataggggac ggcatactgg ttgcccgggt
     1081 cggcggtttc cagcattttc aagagttcgg gatcgatgtt tttatagttg ggaatcaggt
     1141 ctttgttgac tttttgatac gcgcccgcct cgatttggcg cggcaggaag gcgatgcccg
     1201 gcacaaccaa atcgtaaccg gatttgccgg tcagcatttt ggcttccagc gtttcattgt
     1261 tttcgtacaa gtcgtaagtg agcttcagat tgttggcttt tttaaagtct tcgaccgtac
     1321 tctcgtcaac atagttcgac cagttgtaga tgttcagagt atcggtggca gcggcttcgg
     1381 cattggcaac agacgcagcg tctgcttgag gttgcacggc gtttttttcg ctgccgccgc
     1441 aggctgccag agacagcgcg gccaaaacgg ctaatacgga ttttttcata cgggcagatt
     1501 cctgatgaaa gaggttggaa aaaaaagaaa accccgcgcc ccataaacac cccggcgcaa
     1561 ggtttgggca ttgtaaagta aatttgtgca aactcaaagt gatattggac tgatttccct
     1621 aaaaaattac cctgttttca aaagaggggg aaaacgtccg cccgattttg ccgttttttt
     1681 gcgctgtcag ggtgtccgac gggcggatag agagaaaagg cttgcatata atgtaaaccc
     1741 cctttaaaat tgcgcgttta cagaatttat ttttcttcta ggagattcca atatggcaaa
     1801 cagcgcacaa gcacgcaaac gtgcccgcca gtccgtcaaa caacgcgccc acaacgctag
     1861 cctgcgtacc gcattccgca ctgcagtgaa aaaagtattg aaagcagtcg aagcaggcga
     1921 taaagctgcc gcacaagcgg tttaccaaga gtccgtcaaa gtcatcgacc gcatcgccga
     1981 caagggcgtg ttccacaaaa acaaagcggc acgccacaaa agccgtctgt ctgcaaaagt
     2041 aaaagcactg gcttgatttt tgcaaaaccg ccaaggcggt tgatacgcga taagcggaaa
     2101 accctgaagc ccgacggttt cggggttttc tgtattgcgg gggcaaaatc ccgaaatggc
     2161 ggaaagggtg cggtttttat ccgaatccgc tataaaatgc cgtttgaaaa ccaatatgcc
     2221 gacaatgggg gcggagatga atacacggaa tatgcgctat attcttttga caggactgtt
     2281 gccgatggca tccgcttttg gagagaccgc gctgcaatgc gccgctttga cggacaatgt
     2341 tacgcgtttg gcgtgttacg acaggatttt tgcggcacag cttccgtctt cggcagggca
     2401 ggaagggcag gagtcgaaag ccgtactcaa tctgacggaa accgtccgca gcagcctgga
     2461 taagggcgag gcggtcattg ttgttgaaaa aggcggggat gcgcttcctg ccgacagtgc
     2521 gggcgaaacc gccgacatct atacgccttt gagcctgatg tacgacttgg acaaaaacga
     2581 tttgcgcggg ctgttgggcg tacgcgaaca caatccgatg taccttatgc cgctctggta
     2641 caacaattcg cccaactatg ccccgggttc gccgacgcgc ggtacgactg tacaggaaaa
     2701 attcggacag cagaaacgtg cggaaaccaa attgcaggtt tcgttcaaaa gcaaaattgc
     2761 cgaagatttg tttaaaaccc gcgcggatct gtggttcggc tacacccaaa gatccgattg
     2821 gcagatttac aaccaaggca ggaaatccgc gccgttccgc aatacggatt acaaacctga
     2881 aattttcctg acccagcctg tgaaggcaga tttgccgttc ggcggcaggc tgcgtatgct
     2941 cggtgcgggt tttgtccacc agtccaacgg acagagccgt cccgaatcgc gttcgtggaa
     3001 caggatttac gccatggcag gcatggaatg gggcaaattg acggtgattc cgcgcgtgtg
     3061 ggtgcgtgcg ttcgatcaga gcggcgataa aaacgacaat cccgatattg ccgactatat
     3121 ggggtatggc gacgtgaagc tgcagtaccg cctgaacgac aggcagaatg tgtattccgt
     3181 attgcgctac aaccccaaaa cgggctacgg cgcgattgaa gccgcctaca cgtttccgat
     3241 taagggcaaa ctcaaaggcg tggtacgcgg attccacggt tacggcgaga gcctgatcga
     3301 ctacaaccac aagcagaacg gtatcggtat cgggttgatg ttcaacgact tggacggcat
     3361 ctgaaccgcg tgttcagacg gtatatcaag ttgccgtgcc gtctgaagcc gccggcggtt
     3421 tggcggggcg ggataaaaat ttcgtttgaa tccgggcaaa cgcgtataat gtgcggtttg
     3481 tacggacttt gtcggaatca agcaagatga cggaacctgc ggccgaaggc ggtaaagctg
     3541 ccaaggcgtt aaaaaaatat ctgattacgg gcattttggt ctggctgccg attgcggtaa
     3601 cggtttgggt ggtttcctat atcgtttccg cgtccgatca gctcgtcaac ctgctgccga
     3661 agcaatggcg gccgcaatat gttttggggt ttaatatccc ggggctgggc gttatcgttg
     3721 ccattgccgt attgtttgta accggattat ttgccgcaaa cgtattgggc cggcagattc
     3781 ttgccgcgtg ggacagcttg ttggggcgga ttccggttgt gaagtccatc tattcgagtg
     3841 tgaaaaaagt atccgaatcg ttgctgtccg acagcagccg ttcgtttaaa acaccagtac
     3901 tcgtgccgtt tccccaatcg ggtatttgga caatcgcatt cgtgtccggt caggtgtcga
     3961 atgcggttaa ggccgcattg ccgcaggatg gcgattatct ttccgtgtat gttccgacca
     4021 cgccgaatcc gaccggcggt tactatatta tggtaaagaa aagcgatgtg cgcgaactcg
     4081 atatgagcgt ggacgaagca ttgaaatatg tgatttcgct gggtatggtc atccctgacg
     4141 acctgcccgt caaaacattg gcaggaccta tgccgtctga aaaggcggat ttgcccgaac
     4201 aacaataaag ccgccgttca gacggcattt tctgttttca gtttaaatca ataaaaggtg
     4261 attttatgcg taccaactat tgcggtctga ttagcgagca atacttagac caaaccgtta
     4321 ccgtcaaagg ctgggtacac cgtcgacgcg accatggcgg tgtgattttt atcgacctgc
     4381 gcgaccgcga aggcatcgtc caagtcgtga tcgatcccga cacgcccgaa gcgtttgccg
     4441 ctgccgattc ctcccgcaac gaatacgttt tgagcattac cggccgcgta cgcaaccgtc
     4501 ccgaaggtac gaccaacgac aaaatgattt ccggcaaaat cgaaatcctt gccaaagaaa
     4561 tcgaagtgtt gaacgctgcc gctacgccgc cgttccaaat cgacgacgaa aacatcagcg
     4621 aaaacgtgcg cctgaccaac cgcgttatcg acttgcgtcg tccggtaatg cagcgcaacc
     4681 tgcgcctgcg ttaccaagtt gctatgggcg ttcgccgcta cttggacgcg caaggtttca
     4741 tcgacattga aaccccgatg ctgacccgct ccacgcccga aggcgcgcgc gactacctcg
     4801 tgccgagccg cgttcatccg ggcgagttct tcgcgctgcc gcaatcgccg caattgttca
     4861 aacaactgtt gatggtggcg ggtttcgacc gttactacca aatcaccaag tgcttccgcg
     4921 acgaagactt gcgtgccgac cgtcagcctg aattcacgca aatcgacttg gaaacctcgt
     4981 tcttaaacga ggatgaaatc atggacatca ccgaaggtat ggcgaaacaa gtcttcaaag
     5041 acgcgctggg cgtggacttg ggcgacttcc cacgcatgcc ttactctgaa gccatgttct
     5101 actacggctc tgacaaaccg gacatgcgta tcaacttgaa attcaccgag ttgaccgacc
     5161 tgatgaaaac ggaagaattc aaagtcttcc gtggcgcagc cgacatgaaa ggcggccgcg
     5221 tggttgccct gcgtgtgccg aacggtgcga aattcagccg caaagaaatc gacgaataca
     5281 ctaaatttgt cggcatctac ggtgcgaaag gtctggcata catcaaagta aacgatgtgg
     5341 gcaacctttc caacggcgaa gacagcggcc tgcaatcccc gattgtgaaa ttcctgtccg
     5401 aaaacgccct gaaggaaatc attgcgcgta ccggcgcgga aaacggcgac atcatcttct
     5461 tcggtgcgga caaagccaaa gtcgtgaacg aagccatcgg cgcattgcgt atcaaagtcg
     5521 gcttggaaca cggcgcggaa aacggctatt tcacagacga atggaaacct ttgtgggttg
     5581 tcgacttccc aatgtttgaa tacgacgaag aagccgaccg ctacgttgcc gtacaccatc
     5641 cgtttaccgc accaaaagaa ggccatgaag acctgatggt ttccgacccg gcaaattgtt
     5701 tggcgcgcgc ctacgatatg gtattgaacg gctgggaaat cggcggcggc tctatccgta
     5761 tccaccgcgc agacgtgcaa gagaaagtgt ttgccgcgct gaaaatcagc cctgaagagc
     5821 aacaagagaa attcggcttc ctcttggaca acctgaaatt cggtgcgcct cctcacggcg
     5881 gccttgcatt cggcctcgac cgtctggtaa cgctgatgac cggagccgaa tccatccgcg
     5941 acgtgattgc cttcccgaaa acacaacgcg cacaatgcct gctgaccaac gcgcccaaca
     6001 gcgtggacga caagcagttg cgtgaattga gcctgcgcct gcgccaaaag gtggctgaaa
     6061 ccaaagaagc ataaggaaaa cagagccgtt tgacggctct gtttttttca gacggcattt
     6121 acgcttcttg acttccctct aattcaaacc taattttgct gtgttttaat ggcggtattg
     6181 aaaaacacat cttgttcaat caaccgataa aaaaaggact gaaaatgaaa aaactgttat
     6241 tggccgccgt tgtttccctg agtgccgcaa ccgcatttgc cggcgattct gccgagcgtc
     6301 agatttacgg cgatccccat tttgaacaaa accgcacaaa agccgtgaaa atgttggaac
     6361 agcgtggtta tcaggttcac gatgtcgatg ccgacgacca ttggggtaag cctgtgctgg
     6421 aagtggaagc ctataaagac ggccgcgaat acgacatcgt gttgtcttac cccgacctga
     6481 aaatcatcaa agagcagctc gatcgctgac tcctttgatg gcaagctgaa ccaaaatgcc
     6541 gtctgaagcg ttcagacggc attttgcctg ttcctcatca ggtatgaggc aggcttttct
     6601 tattaaaaaa atgacatttc acgctgattt gttataatca ttccttttca acacgacaga
     6661 cggagcaggt ttattatgcc tatccttacc atccgtgaag tgtgcaacat taatcattgg
     6721 ggcataggtt attatgatgt tgacgattcc ggcgaaatca tcgtccgccc caatccggcg
     6781 caacacaatc aaactgcttc actgcaaaaa ctgactgaag ccgtgcaaca aaaacatcag
     6841 gcgcgcctgc ctgttttgtt ttgttttccg caaatcctcg aacaccgcct ccgcgacatt
     6901 aaccgcgcct ttcagacggc acgggaagag tgcggctata agggcggtta ttgtttggtt
     6961 taccctatca aggtcaacca acaccgccgc gtcatcgaat cgcttatgtc aagcggacaa
     7021 ccgcatggtt tggaagctgg ttctaaagcc gaactgatgg cggttttggc acacgccggc
     7081 acccggcaaa cattaatcgt ctgcaacggc tataaagacc gtgaatatat ccgtttcgcc
     7141 ttgatgggcg aaaaactggg gcatcaggtt tatttggtga ttgagaagct gtccgaaata
     7201 caaatggtat tggaagaggc ggaaaaactc ggcatcaagc cccgtttggg tgtgcgcgcc
     7261 agactggctt cccaaggttc gggaaaatgg cagtcttcgg gtggggaaaa atcaaaattc
     7321 ggcttgtcgg cttcccaagt tttgcaactg gtcgatattt tgaaacaaaa aaacaggctg
     7381 gattgcctgc agcttttgca tttccatttg ggctcgcagc ttgggaacat ccgtgatgtt
     7441 gccacaggtg tacacgaatc ggctcggttt tatgttgagt tgcacaaact gggggtaaat
     7501 atccgctgtt ttgatgtagg cggcgggctt ggcgtggatt acgaaggaaa ccgcacacaa
     7561 tcggattgtt ccgttaatta cagcctcaac gaatatgccg ccacagtcgt atggggcatc
     7621 agtcaggctt gtctcgaaca cgggctgccg catccgacaa tcatcaccga gagcgggcgc
     7681 ggcattaccg cacatcacgc cgttttggtt gctaatgtta taggcgttga gcgttacaaa
     7741 ccgcgccggc tggatgcgcc atcgcccgaa gcgccgcgtg tgttgcacag tatgtgggaa
     7801 acttggacgg atatttccgc ctcgcgggaa aaacgttcct tacgcagctg gatacacgaa
     7861 gggcagtttg atcttgccga tgtgcataat cagtataatg tcgggctgtt gagtttggcg
     7921 caacgtgcgt gggcggagca actgtattta aatatctgtc atgaagtcgg cgaattgttt
     7981 aatgaaaaac accggtctca ccgaaccatt attgacgaat tgcaagaacg ttttgccgat
     8041 aagctgtatg tcaatttctc actcttccaa tctttgcccg atgcttgggg catagatcaa
     8101 cttttccctg tttgtcccat taccggtttg aatgaaccga ttgcgcgccg cgccgtgttg
     8161 ttggacatta cctgcgattc agacggtacg attgaccact acatcgacgg cgacggcatc
     8221 gccggtacga tgcctatgcc tgattatccc gaagaagagc cgccgctttt aggctttttt
     8281 atggtgggag catatcagga aatactcggc aatatgcaca atcttttcgg cgacactgcc
     8341 actgccgatg ttgttgtagg ggaagacgga caatttaccg tcatcgatta cgatgaagga
     8401 aacaccgttg ccgatatgct cgaatacgtt tatcaagatc cgaaagagct gatgaaacgc
     8461 tatcgcgaac aaatcgaaca ttcagacctt cctgcctcgc aggctatgtc tttcttaaaa
     8521 gaactcgaag cggggcttaa tggttatacc tatttggaag acgaatagac gcatcaaggc
     8581 atcggatatg ccgtctgaag cccgattttc ttcctcaaac accaatcacc acgaccgatt
     8641 gaaaccaatt acaaggaatc attacgatgc aatacagcac actggcagga caaaccgaca
     8701 actccctcgt ttccaataat ttcgggtttt tgcgcctgcc gcttaatttt atgccgtatg
     8761 aaagccaagc agattgggtc attaccggcg tgccttatga tatggcggtt tcagggcgtt
     8821 ccggcgcgcg tttcggtcct gaagccatcc ggcgcgcctc cgtcaacctc gcttgggagc
     8881 accgcaggtt tccatggaca tttgatgtgc gcgaacgcct gaacattatt gattgcggcg
     8941 acttggtttt ttcttttggc gacagcaggg attttgtcga aaaaatggaa gcgcacgccg
     9001 gcaaattact ttcttccggc aaacgctgtt tgagtttggg cggcgaccat ttcattaccc
     9061 tcccgttgtt gcgcgcccac gcccgctatt tcggcaaact cgcactgatt cattttgacg
     9121 cgcacaccga cacctacgac aacggcagcg aatacgacca cggtacgatg ttctataccg
     9181 cccccaagga aggcctcatc gacccgtccc gttccgtaca aatcggcata cgcaccgaac
     9241 acagtaaaaa attgcctttt actgtgttgt ccgcccctaa agtcaatgaa gacagtgttg
     9301 aagagaccgt ccgtaaaatc aaagaaaccg tcggcaatat gcccgtttac ctgactttcg
     9361 acatcgactg cctggacccg tcgttcgccc ctgggaccgg tacgcccgta tgcggcggct
     9421 tgagcagcga cagggcatta aaaatcctac gtgggctgac ggatctcgac atcgtcggta
     9481 tggatgttgt agaagttgcc ccctcttacg accaatccga cattaccgct ttggccggcg
     9541 ccacaattgc cttggaaatg ctttaccttc aaggtgcgaa aaaggactga acgtccggca
     9601 tcccccgggt tttcgccgtg ccgttcaaac ggcgtattca gtctaatgaa aattcaaata
     9661 ctgaaacaaa agttgcccgg agccgcatat cggaaagacg gtgaaatatc ggaatatatc
     9721 ttataaaaca attagttaaa tattattttt ccgatttttc gggacggtct tttttacgga
     9781 ggtcaatatg atgaaattgg gtttcaaacc gatacccctc gccattgccg cagtattgtg
     9841 cgccctggtt ttggcactgc ccgtacccga cggggtcaag cctcaggctt ggacgctgct
     9901 ggccatgttt gtcggtgtga ttgccgccat tatcggcaag gccatgccgt tgggcgcgct
     9961 gtcgattatt gccgtcgggt tggtcgcagt aaccggcgta accgccgaca aaccgggcgc
    10021 ggcgatgagc gatgcgttga gtgcgttcgc caatccgttg atttggctga ttgccatcgc
    10081 agttatgatt tcgcgcggtt tgctcaaaac agggctgggg atgcgtatcg gatatttgtt
    10141 tatcgccgtt tttggaagaa aaacgctggg catcggttac agtctcgctc tttccgaact
    10201 gctgctggct cccgttaccc cttccaatac cgcgcgcggc ggcggcatta tacatccgat
    10261 tatgcagtcg attgccggca gttacggctc caatcccgca aaaggcacag aaggcaagat
    10321 gggtaaatat ttggctttgg tcaactatca ttccaatccc atttcgtcgg ctatgtttat
    10381 tactgcaact gcccccaacc ctttaatcgt caacttgatt gccgaaaatt taggcagtag
    10441 tttccgtctt tcttgggggg cgtgggcgtg ggcaatggct gttcccggcg ttatcgcctt
    10501 tttcgttatg cctttgattt tatatttttt gtatccgcct gaaattaaag aaacgcccaa
    10561 tgccgttcaa tttgccaaag accgtctgag ggagatgggt aaaatgtcgg cagacgaaat
    10621 cattatggcg gtcattttcg gtatcttgtt gctgttgtgg gcagatgttc ccgcccttat
    10681 taccggcaat cacgctttta gtatcaacgc caccgccacc gcatttatcg gattaagcct
    10741 gcttttgctt tccggtgtat tgacttggga cgatgttttg aaagaaaaaa gtgcgtggga
    10801 tacgattatt tggtttggcg cattgattat gatggccgca tttttaaata aactcggact
    10861 gattaaatgg ttctccggag tgttggcgga aagtgtcggc ggtttgggcg ttagcggcac
    10921 ggctgcgggc gtaatcctcg tgcttgctta tatgtatgcg cattatatgt ttgccagtac
    10981 tactgcacat attaccgcta tgttcggcgc atttctcgct gctgccgttt cactgaatgc
    11041 ccctgcgatg ccgaccgcgc tgatgatggc ggccgcatcc aacattatga tgaccctcac
    11101 tcattatgcg accggtactt cgcctgtgat tttcggttcg ggctacacca caatgggaga
    11161 atggtggaag gcgggtttta tcatgagcgt agtcaatttt ctgatttttt tcgttatcgg
    11221 cagcatttgg tggaaagttc tggggtattg gtaagggaaa aataaaataa atttccaatc
    11281 tgtgtttatt tgattgggcg actattatcg tgaaatatgc cgtctaaagc cttcagatgg
    11341 catatttgtg cgcttgaatg ttgcagaaag cggcaggcgg cggtgtagga aaagccaaac
    11401 aaaaaccaaa ccgcctatca acttctgata aacataagca ttaaataatc agaaggttat
    11461 tcaattacct aaacgcaaat ttcccttacc taaacgcaaa tttccctgcc gtatcacatc
    11521 tattgaaaat aatacatcaa ccggctcgga agcagcctga tcaggtgttt ctacttgcgg
    11581 cgatgaatcg gcagccggtt cggtataggc agtcggcgtg ccgtcggatt gggtatttaa
    11641 atgcggttgt gcctcgggcg tgtgtacatc aggcacttcg ggcgtgcgtg tctcggatat
    11701 ttcggcagag ttggtttcct cagtttgttc aatgacttca gcttggctgt atgaggaaga
    11761 accctgtatc cacgccagcg atttgagcgg catcttcatc ttgccgtttt tgccgcaggt
    11821 caggcagacg gccgatccgg tgcggtcttt gagtacagca tcgactttct cgggcgcgat
    11881 ggttttaccc tgtgtttttg cccattgcgc aatacttttt ttcagagagg cgatgtcaag
    11941 gttgttctta ccgtaagggt cgcggaaggc ggcaagccac aggttgcgat ccgcatcttc
    12001 attgagaccc gccatttgat agatgacgga ggcgggcgag ccgctggcga tggttttggc
    12061 aaactcgagc gcgtcgggcg atttcatgcg gacgcagccg tgactccgaa ccccggggac
    12121 gctggccggc gcattggtcc cgtgtatgcc caaaccgagt ttggggtcgc ctaagcggac
    12181 aaaaaccggc cccaaagggt tgtccgggcc ggcggctatg gtttttacgc cgtcgccgcg
    12241 ttctttctgt atggatttgg ggatgtacca aacagggtta taggctttcg ccccgatttt
    12301 atgttcgcct agattggttt gcgtcatcgc ccgacctacc gcaacgggat aaaccttggt
    12361 cagtttgccg tcggtgtaga ggaacaggcg ttgctgaggg atgttaatga agacatgttg
    12421 accttgtgcg acgggggaga catcgggaat gacggtgttt gcgtatgaaa aaccgcttat
    12481 caatagtgca gcagtgcggc agattgtttt attcatatca aaatatggtg tgtgtccgat
    12541 aggttttcgg caaatcatac ctgaaaccgt accaatttgt gcgaaaatat gcgcttcggt
    12601 acagtgcgga cggattgagt gatggcaacg gaaacaaatg tcgcggaaat ttccgccttg
    12661 gattatgaag gcaggggtgt ggcaaaggtc ggcggcaaaa cggtttttat taaaagggca
    12721 ttacttgatt gtttgatgct gggttggttc aggctttaac tcaggaatat ttacatcata
    12781 atgaaggttt ttaaacaaca gcttgaacaa ctcggcgcgc aaaaccaata tcgttcgatt
    12841 ccggatttga ttcatcaagg gcggtatatt acgcgggaaa actgcaaaat gctgaatatg
    12901 tcgtctaatg attatttggg tttggcatca gatgaaaact tgcgccggtc ttttttgcag
    12961 caatacggcg gtaattttcc ctcttttacc agttcttcat cgcgtttatt aacgggcaac
    13021 tttcctattt ataccgattt ggaagagctt gtcgcacaac gtttccaacg ggaaagcgcg
    13081 ttattgttca acagcggcta tcacgccaat ctcggtattt tgcctgcttt gacgacgacg
    13141 aaaagtttga ttttggcaga taaatttgtt cacgccagta tgattgacgg catccgtttg
    13201 agccggtgtg cgtttttccg ttatcgtcat aatgattatg aacatttgaa aaatctgctt
    13261 gaaaaaaacg tcggaaaatt tgaccgcact tttatcgtta ccgaatctgt tttcagtatg
    13321 gacggtgatg tggcggattt gaaacagctt gtccaattaa aaaaacagtt tcccaatatt
    13381 tatctttatg tggatgaagc ccacgcaatc ggtgtttatg ggcaaaacgg attggggatt
    13441 gccgaacggg ataatttgat tgccgagatt gatttattgg tcggcacttt cggtaaagcc
    13501 ttagcctcgg tgggggcgta tgccgtctgc aaccaagtat tgaaagaatg tttgattaat
    13561 caaatgcgcc cattgatttt ttcaaccgca ttgccgccgt ttaatgtggc ttggacttat
    13621 tttatttttg aacgattgcc gcaattctca aaagaaagaa gccatcttga gcagttaagc
    13681 gcatttttac ggcaggaagt ggcgcatcgg acgcaaataa tgccgagcca aacctgtatc
    13741 gtcccctata ttttaggcgg gaatgaagcc gcccttgcca aagcggaata cctgcaaagg
    13801 cagggttatt attgcctgcc catcagaccg ccgacagtac ccaaaaacac atccagaatc
    13861 cgcctgtctt taacggcaga tatgacaacg gatgaagtgc ggcagtttgc ggcgtgcctg
    13921 taaggatatg atatgaaaac aaaattttac gactgccaag gcgaacattt aattctgtat
    13981 tttgcaggtt ggggaacgcc gcccgatgct gtaaatcatt tgattttgcc gacaaatcac
    14041 gatttattga tttgctatga ttaccaagat ttacgtttgg attttgattt ttcctcctat
    14101 cggcacatcc gtttggtggc gtggtcaatg ggcgtttggg cggcagagag ggcattgcaa
    14161 ggaataagac taaaatccgc aacggcagtg aatggcacag gtttgccttg cgatgataat
    14221 ttcggtatcc cttgcgccgt ttttaaaggc acattggaga acctcacgga aaatacccgt
    14281 ttaaaatttg aacgcagaat gtgtggcgat aaagcatctt ttgaagatta ccaacaattt
    14341 cccgcacgcc cgtttgacga aattcatcaa gaacttaccg cactttttgc gatgatcggg
    14401 caagataggc gtacagatct tatctgctgg acaaatgcct tggtcggatc gggcgataaa
    14461 atttttatgc ctgccaatca gcaccgatat tggacaccgc gttgcaccgt tcgggaaatt
    14521 gacggcggac attacctgtt ttcaagattc acccattggt cggcactatg gaatcactga
    14581 ctgccataaa taaatcgcgc attcggcagg ctttccaaaa agcattaaac gattatgacc
    14641 ggcacgcctt aatccaacaa aaaatgacga ttaatttaat gacgcatttg caagattatt
    14701 tgccggatat gccattggaa aacgtgttgg aattgggctg cggctcagga atgttgagtg
    14761 ccttgctgca aaaacagatt tcagcgaatt attggttatt taatgatttg tgcaatgtgc
    14821 agccccaact ggctgaaaaa ctgccgcaat cctttgattt ttattgcggc gatgcggaaa
    14881 actttccttt tcaacgacaa tttgacttaa tcgcaagcgc atctgccgtg caatggtttc
    14941 atcaacccga cgcttttatc acccattgca aaacaggctt gaaaacaaac ggattattgg
    15001 cggttgcaac ctttggcaaa gacaatttaa aagaagtccg ccaaattaca aatataggct
    15061 taaattaccc gactttatcc caatggcagg cttggttagc caaagatttt gagcttttat
    15121 ggtgtgagga ttttacggta atactagact ttgatacgcc gtcagatgta ctcaaacacc
    15181 ttaaatatac aggcgtaaca gccacgaacc aaaaaaattg gacaagaaaa aatctcaatg
    15241 gatttattgg cgattacttg tcggcgttcg gtatgccgtc gggcaaagtg cgcctgactt
    15301 atcatccgtt attttttatc gcgcgctact ctgcggcggg caggcaataa ggcagcttat
    15361 gggcaaagtt atttttatat cgggtattga tactgatgtg ggtaaaaggt aatatggcga
    15421 ggcttgtgca gaaggcatat tgttaaacgt taaattatgg tatgatttaa aacttacaag
    15481 tctatttcag taaatcgtta ataataaaag cggacaatgg ccgttgcagg cggtcagcta
    15541 tccgctcgtt acggtatcgg gagtatcctt tggaaaactc attaattatc ggtttggcac
    15601 tggcggtatt gctgatgctt ttggttatgc gtgcgaagca gggtaagaaa acaccgaagc
    15661 gcaaatccca aggtgtcggc aatacgcagg ggcagacacc cggaagcaat gattcggact
    15721 gggtcgatca gattggacaa tcggtttcag acggcacgca acccgactgg tcttggaacg
    15781 aaagtgccga gaccgcatcc gccgccgtat ccgcgcaaga agtcgatccg cttacggagt
    15841 atcaggttta taagcaattc ggttatcagg gcaaggctgc cgaatctttg gctgcctatc
    15901 tggacggcat tccggatggt gaagcgaaac ctgaaaacct tatccgcgag ctgctcgata
    15961 tcaatctcga agtgggggat gtcgatgttt tggcagacaa tctgcaaaaa tacggcaaac
    16021 tgattccttc cgaacttttg gcaaaatata tcgaacaggc attacagcgc gattcaaacc
    16081 atttgcgtat ccgcgtcttg gcggaagaag gtttgggatg gggtactcag gagattgaaa
    16141 aacgtgcgga aggcggttct gcgacggcag cttccgcatc gcccccgccg gatgccggcg
    16201 gtaaggctta tgaagccgaa gaaatcaagc gcatcccgat tgtgcggggc aaaaaagacg
    16261 tgtccggaat cagtcaagag gaaatcggtg cgattgccgg tttggtccgt gccgatcaag
    16321 gtgcgaaaat ccttaaagac aaagtcagct atgaaacggc atcgaaacaa tacgaccgtg
    16381 ccatccaaac ttccgaaaaa cctgcaaacc tgattatcga tgcgttgaaa ctcgattacc
    16441 aatacgcgga catagaccgt tttgccggac atttgtggaa actttaccaa acgttgggca
    16501 actacggcag gcaggttaaa gagcggatgc tggggtgggg gtacagcctg ggttaccatg
    16561 aggttttcga tgatttggaa aaagggccga acgaccggca aatcaaagac atcggtatgg
    16621 ggcacgggta tctgccgaaa aatatacaga aattcaaatc gcaacatcgg gatttggtgc
    16681 ttcaagattc ttcgttgatc aacaccggtt cgtctccggc agacgatgcg gttaaggaag
    16741 tagagtcgtt gctgatgtat ggtcagattg aagcggcaat ggatgtgttg gagcaggcgg
    16801 tattgaaata tcccgacgag tcccagcttt atattacgtt gatcgatatt tatgaacgta
    16861 ctgaagattg ggataggttg gggcagtttt taagggtatt gagggaacgt gcggacaggc
    16921 ttcctgaaga ggtcgttatg ctgatgagcc ggctgctgca gcgtatgaat caaaatatta
    16981 aaaaaataaa acggtacgga aaataaaaat ggaagttcaa ctgccgaaaa ttaaaacagt
    17041 acgcgtaatg ttggcgggga tgacggcgca gcaggaatcc gttttcaaaa tggcattcaa
    17101 aatgcacaat accacccgtt atgaaacagt atccccttca gacggcagtg ccgtgcccga
    17161 tttggttttg gcggataccg atgccgaggg cggttttgaa ctttggaaag agcttgccga
    17221 gcgttataag gatatacccg tcgccgtctg ttcggagaaa gttcccgatt ctgaagttcc
    17281 ctacctgccc aaaccgattc ggtttgaaac attgtttcct atgctccgca aattgttgca
    17341 gggcgagaat gtttatggga aatcgtttat tgcacccgca gaccggtcgg cgaaaaataa
    17401 cgggaatgtg cagcgtacgg ttacgatacg ccagtttaac ccgaataaag gattattggg
    17461 ggcgttgcgg tttgcggaaa agaacaggca ggacatcgct atcttgcatg gaaataagcc
    17521 ggtccttatt gttttcccct cgatacaacg ggttttgctg acagaaagtg tgcaaaaact
    17581 cgaagaattg tgcaaagacg aaaatttgca ggtcagctgc aagactgttc ccgataaccc
    17641 gcaatggcgc gaaaaggcta aagtaggcat tatgtcctgt atgtggcagt tttccatttg
    17701 gacggcgcag ggcaggttga tttatccgat ttctcccgat actccgttta cgttgaaatc
    17761 ttggccaaac ctgacccggt tggcaaatgt gccggggtcg atacgcttgt cggcatttct
    17821 gaccaaggca tccgtcaacc ttaacgtgtt gtataaagtg atgcctttaa acctcaatga
    17881 tattctgaat tatcttgcgg caacctatac aaccgggttt ttgtcggtag atttaaaaac
    17941 ggtttcacaa caggcatact ccgatatggc ggataaaata aatatcggag ccgattctgc
    18001 ctctgatagt gaaatgatga aaaaagcgga aaaaatcaca acaccatccc aatcccagtc
    18061 gcgcggtctt ctgcaaaggc tgatgaaaaa actgttgggc agctaagagg cggagagatg
    18121 agagaaaata aaattatttt cacaggacct gtcggcgtag ggaaaaccac tgccattgcg
    18181 gctatttcgg acgaagcact cgttcagacc gatgcttccg catccgatat gactttggat
    18241 aggaaaagga atacgacagt ggcgatggac tacggggcca tcagcttgga tgaggatacc
    18301 aaagtccatt tatatggtac gcccggtcag gaacggttca actttatgtg ggaaatctta
    18361 agccaaggca gtatgggttt ggtcttgctt ttagataatg cccgaaccaa tccgttgaaa
    18421 gatttggaat tctttttaca ttcgtttcga gggctgctgg agaaggcacc cgtcgttgtc
    18481 ggtattacca agatggatat acgctctcag cccggtatcg acgtgtatca caaatatctt
    18541 gcaaaacata atcttaatgt tccggttttt gaaattgatg cccgtaagga agatgacgta
    18601 aaacaattgg ttagcgcaat gttattttct attgatccgg gactggaggt ttaatatgga
    18661 atcaacactt tcactacaag caaatttata tccccgcctg actcctgccg gtgcatttta
    18721 tgccgtatcc agcgatgccc ccagtgccgg taaaactttg ttgcacagcc tgttgaaagc
    18781 ggatgcggac gaaatggtca gcagtgagaa gctgcttact tgggcggaca ccgccgacat
    18841 cgataccgct ttgaacctgt tgtaccgttt gcaaaaactc gaattcctct atggcgatga
    18901 aaacggtcat tcagacggca tcaatttgtc ggacgagcaa ttgccgttgc tgatggaaca
    18961 attgtccggc agcggtaagg cgttattggt cgatcggaac ggtctgtatc ttgccaacgc
    19021 caatttccat catgaggcgg cggaagagtt ggggttgttg gcggcagaag tcgcacagat
    19081 ggaaaagaaa taccggctgc tgattaagaa caacctgtat atcaacaata acgcttgggg
    19141 cgtttgcgat ccttccggtc agagcgaatt gacatttttc ccattgtata tcggttcaac
    19201 caaatttatt ttggttatcg gcggcattcc cgatttgggc aaagaggcat ttgttacttt
    19261 ggtaaggatt ttataccgcc gttacagcaa ccgcgtgtaa aacttgggag agaggagggg
    19321 ttatgcagca attattgatt tcaatccttg aagatttaaa caatacatct acggatatta
    19381 tcgcgtctgc cgttatctca accgacggat tgccgatggc gacaatgctt ccttcacatt
    19441 tgaattcgga cagggtaggg gcgatttctg ccactttgct tgctttgggg agtcgctcgg
    19501 tgcaggaact cgcctgcggg gaattggaac aagtgatgat taaaggaaaa tcaggctata
    19561 tccttttaag tcaggcgggt aaagatgccg tgttggtgct ggtggcaaaa gaaaccggca
    19621 gacttggttt aatcctattg gatgccaaac gtgcggcaag gcatattgcg gaagccatat
    19681 aacatataaa gattgcgggc ttgcagataa agtgcaatcg attgtcaatt tatattgaca
    19741 cgttcggtat ttctgtttta ttattcgcgc ttattccccg atagctcagt cggtagagcg
    19801 acggactgtt aatccgcagg tccctggttc gagcccaggt cggggagcca aatttcaaaa
    19861 ccctctaagt attttcttag agggttttgt tttaccggcg gtcagaaacg catttttgag
    19921 atgattgttt tgagatggaa taaaatcttt gcaaaattcc tttcgtgatg gttatgaaaa
    19981 aataggggct gtcctggaca actaggataa actcgatttt atagtggatt aacaaaaacc
    20041 agtacggcat tggctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
    20101 agtgaatcgg tttcgtacta tttgtactgt ctgcggcttc gtcgtcttgt cctgattttt
    20161 gttaatccac tatactaatt gagacctttg caaaattcct ttccctcccg acagccgaaa
    20221 tcccaacaca ggttttcggc tgttttcgcc ccaaacacct cctaattcta cccaaatacc
    20281 cccttaatcc tccccggata cccgataatc aggcatccgg gttacctttt aggcggcaac
    20341 aggcgcactt agcctgagac ctttgcaaat ttgtcggttt cggggtcgta ttggtagcct
    20401 cgtgcctgta tgtcttcttt gaaagtttcg tatacgtcat gggctaaaag ggctgttccg
    20461 acatagggaa ccgcccttgt gctgaatttt gcgcctaagc gggcaagttt gccgaccccc
    20521 gccaatacgc cggcgcggga aacgctggcg gttattttgg cgttgattcg ggcttttgcg
    20581 cccgtaggga tgtgctcaaa atttgccgct tctatgaatt tggcaaaact cttagaactt
    20641 gaatctaatt taaaaccact tgattttaaa ttattacttg attgaaattt aatttgtgat
    20701 ttttctaatc ttacaggttc agaataagaa ttactcgaac atagaattaa aatcagaact
    20761 atcggtgtac tcttgataaa caaattcatg aacagttttt ccgttctctt ctgattttct
    20821 tataaaaata acagattcat cagaaaaata aatcttccaa atattatgtg cgactctctt
    20881 atttaaaaaa taagagaagc tctctaaaat atcgtactct tttacgttat taacaaattc
    20941 ttcaaattct ttctgagcaa ttaa
//
LOCUS       DAWFIT010000029        20926 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFIT010000029 DAWFIT010000000
VERSION     DAWFIT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.029"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGQ85_001019"
     CDS             complement(446..3235)
                     /gene="ileS"
                     /locus_tag="WGQ85_001019"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0750461.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGQ85_001020"
     CDS             complement(3376..4296)
                     /gene="ribF"
                     /locus_tag="WGQ85_001020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0750462.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIEADMEAAKCW"
     gene            complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGQ85_001021"
     CDS             complement(4823..6118)
                     /gene="tyrS"
                     /locus_tag="WGQ85_001021"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0750463.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(6181..7971)
                     /locus_tag="WGQ85_001022"
     CDS             complement(6181..7971)
                     /locus_tag="WGQ85_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0750464.1"
                     /translation="MAAGRVSRCRYILCDFRLFDDDDSSPRNVGGGGRFSLKAFYIRR
                     IKRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQ
                     EEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFD
                     KYYLPHLRACEMLVGSLTAVWMRYRQQRNLAVGRCCAAAGALFSACILSACLFAYSEQ
                     TAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAF
                     MRYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFAQSVLWIYALPMLIL
                     GAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHY
                     KTFFDAVGKKEKWAATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPRYSKVV
                     LAMRWGSQMPENSRSLAYDAGFFKKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYI
                     LSSRIPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPANFQIGGLPVYS
                     DKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(8071..8175)
                     /locus_tag="WGQ85_001023"
                     /pseudo
     CDS             complement(8071..8175)
                     /locus_tag="WGQ85_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8427..9518)
                     /gene="ychF"
                     /locus_tag="WGQ85_001024"
     CDS             complement(8427..9518)
                     /gene="ychF"
                     /locus_tag="WGQ85_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0750465.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9631..11307)
                     /locus_tag="WGQ85_001025"
     CDS             complement(9631..11307)
                     /locus_tag="WGQ85_001025"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0750466.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEYGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            11482..11913
                     /locus_tag="WGQ85_001026"
     CDS             11482..11913
                     /locus_tag="WGQ85_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0750467.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(12144..12839)
                     /locus_tag="WGQ85_001027"
     CDS             complement(12144..12839)
                     /locus_tag="WGQ85_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0750468.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12857..13357)
                     /gene="hpaC"
                     /locus_tag="WGQ85_001028"
     CDS             complement(12857..13357)
                     /gene="hpaC"
                     /locus_tag="WGQ85_001028"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0750469.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(13472..13912)
                     /gene="nadR"
                     /locus_tag="WGQ85_001029"
     CDS             complement(13472..13912)
                     /gene="nadR"
                     /locus_tag="WGQ85_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0750470.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(14255..14764)
                     /locus_tag="WGQ85_001030"
     CDS             complement(14255..14764)
                     /locus_tag="WGQ85_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0750471.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14843..14918)
                     /locus_tag="WGQ85_001031"
     tRNA            complement(14843..14918)
                     /locus_tag="WGQ85_001031"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14883..14885),aa:Lys,seq:ttt)
     gene            15129..16208
                     /gene="apbC"
                     /locus_tag="WGQ85_001032"
     CDS             15129..16208
                     /gene="apbC"
                     /locus_tag="WGQ85_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0750472.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGATAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     repeat_region   16825..17063
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16893..16918
                     /rpt_unit_seq="ccgtcattcccgcgcaggcgggaatc"
     gene            17391..20521
                     /locus_tag="WGQ85_001033"
                     /pseudo
     CDS             17391..20521
                     /locus_tag="WGQ85_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
BASE COUNT         5008 a         5654 c         5562 g         4702 t
ORIGIN      
        1 cactatagat tcccacttcc gtgggaatga cggttcagtt gcattccgac aacaccgtaa
       61 tcttgaaatc cgtcattccc gcgcaggcgg gaatccagac ccctgacgcg gcgggaatct
      121 atcggaaatg actgaaaccc cgagattcta gattcccact ttcgtgggaa tgacggttca
      181 gttgcgttcc gacaacaccg caatctcgaa atccgtcatt cccgcacagg cgggaatcca
      241 gacccacgac gcggcgggaa tctatcggaa atgactgaaa cctcgagatt ctagattccc
      301 actttcgtgg gaatgacggt tcagttgcat tccgacaaca ccgcaatctt gaaacccctc
      361 cgccgttata aagacaaatc acggcacaaa aaatgccgtc tgaaatgctg ttcggcggtt
      421 tcagacggca tttgctcaaa ctttatcagg cgtaatggcg cgtttcgcct tctccgccga
      481 cattctctgc acagcgtttg cagacggttt catagcctgc aaccgcgccc acatcgcggg
      541 tgtagtgcca gcagcgttcg catttttcac catcactggc tttagcggca acggcaagtt
      601 cgctgcctac tttcacttct gctttagaca ccagcaaagc aaagcgcaat tcttcgccca
      661 aagcattcag atagccggcc atttcttccg gcgcggtaat ttcggcttcg gcttgcaagg
      721 acgaaccgac ggttttgtcg gcgcgcaaag gctcgatggc ggcggttacc gcttcgcggg
      781 cttcgcggat tgccgtccat tttttcacca gttcggcttc ggctttttcg ttgatggtcg
      841 ggaactcgtg ccaggtgtgg aagaggacgc tgtcttcttc gccgccgccg atgatgtccc
      901 acgcttcttc gccggtaaag cacaaaatcg gcgcaatcaa gagaaccaaa ctgcgcgtga
      961 tgtggtacag ggcggtttgc gcgctgcggc gggcgcggct gtcggctttg gtggtgtaga
     1021 ggcggtcttt caggatatcg aggtagaacg cgcccaagtc ttccgagcag aaagaaacaa
     1081 tgtctttcac ggcaaaatgg aaggcataac gcggatagta atcgcctgcc agacgctctt
     1141 gcagccgacg cgccaatacc acggcgtagc ggtcgatttc caccatgtct gcctgttgca
     1201 cggcatcttc aataggattg aagtcgctga ggttggcaaa caggaagctc aaggtgttgc
     1261 ggatacggcg gtagctttcg gttacgcgct tgaggatttc tttggaaatc gccaattcgc
     1321 cgctgtaatc ggtggatgcc gcccacaggc gcaggatgtc cgcgccgaac tcgttataca
     1381 cttcttgcgg cgcgacgacg ttgccgatgg atttcgacat tttgcgcccg ttttgatcca
     1441 ccacgaaacc atgggtcagc agctgtttgt acggcgcgcg acccattgat gaggcgcagc
     1501 cggtcagcat ggacgactga aaccagcccc ggtgttgatc gctgccttcg aggtacaagt
     1561 cagcaggcca gtccaattct tcgcgctgtt tcacaacgga ataatgggtc gaaccggagt
     1621 cgaaccatac gtccatcgtg tcggagagtt tgtcgtaatg ttcgcaatct tccgcgctca
     1681 acaattcgcc cttatcgagg gagaaccaag cctcgatgcc tttttcttcg atgcgttgcg
     1741 cgactttttc caaaagttcg gcagagttcg gatgcagctc gcccgtttct ttgtgaacaa
     1801 agaaagtcat cggcgtgccc caatagcgtt ggcgcgaaac cacccagtcg gggcggcctt
     1861 caatcatggc ttccaaacgc gcacgacccc atgatgggaa aaattcagtg tcatcaacgg
     1921 ctttgatggc tttatcgcgc agggttttac catcggcacc tgctttatcc ataccgacaa
     1981 accattggcc ggtcgcacgg taaatcaacg gcgttttgtg acgccagcag tgcgcgtagc
     2041 tgtgttcgat tttgctgctt gccaagaggt tgccgatttc ttccaaccat tgcaggataa
     2101 cgggattggc ctcccaaacg ctcatgccgg caacgcgcgg cgtttcactg atgtatttgc
     2161 cttcggcgtt gacggggttg taaagctcga tgccgtattt attgcagacg gcgtagtctt
     2221 ccaaaccatg agcaggcgca gtatgcacca aaccggtacc ggcgtcggtg gtaacgtggt
     2281 cgccgttgag catgggaata tcgcgttcga ggaacggatg attcatgtgc aggttttcca
     2341 gcttgtcgcc ggtggtttcg gcaagaatag caatgccgtc tgaaaaaccg taacgtttga
     2401 gcgcgtcttc tgccaaatct ttcgccagca ccaatttgcc tttgggcgta tcgattaatt
     2461 gatacaccac gtccgcaccg gcagacacgg cttggctcgc tggcagcgtc caaggcgtgg
     2521 tcgtccagat gacggcaaac gctttgccct cgatgcctgc caaaccgaat gcagcggcaa
     2581 gcgcagcggt gtctttaaac ggatagccca cgtcaatcgc aggcgatact ttgtctttgt
     2641 attccacttc ggcttcggcc aatgaagagc cgcagtccaa gcagaactga accggtttcg
     2701 cgccgcggta gagatagccg gatttgtaga tttcgccgag catacgcacg gtatcggctt
     2761 cggttttaaa atccatggtc aggtagggct tgtcccagtc gcccaacacg cccagacgga
     2821 taaagtcttt tttctggcgg gcaacttgtt cggcggcata ttcgcggcac aattcgcgga
     2881 aacgcgcttt gggcatgtct ttgccgtgca gcttttctac catcacttcg ataggcaaac
     2941 cgtgacagtc ccaacccggc acataaggcg cgtcaaaacc ggcttgggtt ttgctgcgga
     3001 taatgatgtc tttgagaatt ttgttgacgg cgtgaccgat gtggatgtcg ccgttggcat
     3061 acggcgggcc gtcgtgcagg atgaacttcg gacgaccttt ggcgatttcg cgcagttttt
     3121 ggtagcgttt ttgctcgtac cagcttttca gccatgcagg ctcgcgcttg gcaagattgc
     3181 cgcgcatcgg aaacgggctt tcgagcaggt ttacggtttt gctgtaatcg gtcatttttt
     3241 aatctctatt gttacaatat ttcggttcag acggcattgc gcctcaaaca gtatttcaca
     3301 acgggaaacc cctatgccgt ctgaacagtt aaaaggttga ttgtagccca atcggatggt
     3361 ttgtataagg tttttctacc aacactttgc ggcttccata tcggcttcaa tctgcctttt
     3421 cagttctgcc ataccgtcaa acttttcctc atcgcgcagt ttgtgcagga agcggacgtt
     3481 caaccgttgt ccgtacaaat cgccttgaaa gtcgaacagg tggacttcaa gcttttgaga
     3541 acagccgcta tcaacggtgg gattgaaacc gaagctcgcc acgccgcgcc gcgtgccgaa
     3601 tgcgccgtcc gcttcgacga caaacacgcc gccgagtgca taacggtggc ggggcaggcg
     3661 gatgttggca gtcggggcgt ttaaggtgcg tccgagtttt ctgccgtgca ccaccctgcc
     3721 gctcaagacg tagtcgtgtc ccaaaagttt tttcgcatag gcaaggttgc cgtctgaaag
     3781 ggcttgtcgc acggcggtac tgctggtgcg gatgtcttcg acgatgacgg aaggcgtacg
     3841 ctcggtctgc atatcgggct gttgtgccaa aagttcaaaa cagccttccc gccccgcacc
     3901 gaaacggaaa tcgtcgccga cgagcaaata acgcgtattc aaggtttgac gcagcaggcg
     3961 gtcgataaac ccttgcgcgg atatttcgga aaaattttga tcgaaacgta aaacccagac
     4021 ggcatcgacg cagcccgtcc cttccagcaa ttccagtttg gtacgcaggg ggctgatgcg
     4081 gcagggcggg gttttgcctg cgagggcggc aaaaaattct ttgggttgag gttcgaagat
     4141 gacggcaacg acgggcagtc cgcgcgtatc ggcttcgagg cggagttttt ggaggatgtg
     4201 tttgtgtccg aggtgtacgc cgtcaaaatt gcctatggtt acggcggccc cgtgcggaaa
     4261 gtcgggcgcg ttgtgccgcc ccagcctgat tctcattgtt atgttcgggt atgtggtgaa
     4321 acaggcggtc attgtaaacg gtattgcggt ttatagacag tgtgccgccc gttacgcccg
     4381 cccgacgcgg gaaaagtagg caaatttccc gcagccgaac gcgccaaacg cataaagcgc
     4441 gcagcaggcg cggtgctatg tgttgaaaca tcgccccaaa cctgatgcaa catcagtgaa
     4501 aatcgttctt ttttaaccag gtaaaccgag acaaacaacc ctccgccgtc attcccgcgc
     4561 aggcgggaat ccggacctgt ccgcacagaa acttatcgga taaaaacagt tgctcaaacc
     4621 ccgagattct agattcccac tttcgtggga atgacggttc agttgcgttc cgacaacacc
     4681 gtaatcttga aactcgtcat tcccgctcag gcgggaatct agaacgtgga atctaagaaa
     4741 ccgttttgcc cgataagttt ccgtgcggac aggtccggat tcccgcctgc gcgggaatga
     4801 cggcatttct gcggcaatcg gattatttcc aaaccaaaag cgcgtgattg cgtttgccgc
     4861 gccgaaggat ggtgtatttg ccgaaacgtt tgtgttcgtc ggtcagcagg caggcatcgt
     4921 cggggcgttc ggcggcgtgg ttggggttgt tggcttcggc aggtttgccg ttgagcaaaa
     4981 ccgctttgct gttcacaaag ccgcgcgctt ctttattgga ggatgccaaa ccggttttga
     5041 ccaaggcttc gacgacattg atgccgtctg aaacttcaaa tgcaggcagg ccgtcgaggg
     5101 cgagctgctc gaagtcgctt tcggtcaggc tgctttggtc ttcggcaaac aggctttcgg
     5161 aaatgcgctg cgcggcggca agggcttctt cgccgtgaat caggcgggtc atttcttcgg
     5221 cgaggatgcg ttgcgcttcg ggcttgctgc cgcttgcctg gtctttggct tcgacggcat
     5281 cgatttcttc gatggacagg aaggtaaagt atttcaggaa tttatacaca tcggcatcgg
     5341 cgactttcag ccagaattgg tagaactgat agggcgaggt ttttttcgcg ttcagccata
     5401 ctgcgccgcc ttcggttttg ccgaatttgg tgccgtctga tttggtaacc aaaggcaggg
     5461 tcaggccgaa cacttgtttt tgattcaggc ggcgggtcag gtcgataccg gcggtgatgt
     5521 tgccccattg gtcggagccg ccgatttcca aaaccgcgcc gtggcgtttg ttcaactcgg
     5581 cgaagtcgta gccttgcagc agggagtagg cgaactcggt gaaggaaatg cctgcgccgt
     5641 cgcggtcaat gcgctgtttg acggattctt tgttcagcat ggcgttgacg gagaaatgct
     5701 tgccgatgtc gcgcaggaag tcgaggcagt tcattttgcc gaaccagtcg gcgttgttcg
     5761 ccataatggc ggcgtttccg ccttcaaagc tcaagaaagg ggttaattgg ctgcggatgc
     5821 tttccaccca gccggcaacg gtttcggcgg aattcaagct gcgttcggcg gctttgaagc
     5881 tggggtcgcc gatcataccg gtcgcaccgc ccaccagtgc aatcggcgta tgccccgcct
     5941 gttggaagcg gcgcaatgcc aatacgggca gcaggtgtcc gatgtgcagg ctgtcggcgg
     6001 tcgggtcgaa gccgcaataa agggaaattt tttgttcgtt caacaaagcg tctaaggctt
     6061 cgatgtcggt ggtttgcgcg ataaggccgc gcgattgcag gtcttggatg acgctcatcg
     6121 gtctctttca aaaaaaatta gcggttttgc aaaccgccga ttgtaacaaa tttaagcgaa
     6181 tcaatggtta tggcgcgtat cgagaaaccg ttgtttttcg gaaaaacgct tcgccaattc
     6241 cgtgccgccg taagggttga tgtggtcttt gtccgagtaa accggcaatc cgccgatttg
     6301 gaaatttgcg gggatatagg cggcggcatc aataatatag acgttggggt atttggctgc
     6361 caattccctg atgcgtgcat tggctttcag agtgctttcg tcgtccgggc gcagggtttg
     6421 gcggtaaccc ggtatgcgtg aagacaagat ataggcgcgc tggacgttgt aagacgaggc
     6481 aaggttgtcc gccatcaggt aaacggcttg tttttcggac gagagtttat gcagcatacg
     6541 gtcgaatttt ttgaaaaaac cggcatcgta ggcaagggag cggctgtttt cgggcatttg
     6601 gctgccccac cgcatcgcca aaaccacttt tgaataccgg ggcaggtgtt cttcggcata
     6661 gcggtaaacg gcgcggcagg cggcccagtt ttggaatacg cgggacgcgt agccttccac
     6721 ataggcgcag gcgtcggcgg aaaccatagt ggcagcccat ttttcttttt tgcccacggc
     6781 atcgaagaat gttttgtaat ggtcggcgtg ggagtcgccc aaaaccagca gttccggctg
     6841 tttttccgta tccccccata ggcattgttt gccggtattg ttgtggcagg aggtgttgga
     6901 acgcgtcagc cccaagcggt cgtattgcgc cataaacggc agcctcatcg caaaaaatga
     6961 gcccgccccc aaaatgagca taggcaaggc ataaatccat aaaacggatt gtgcgaacga
     7021 accttgccat tttttaaacg gtttttcgat gcagtggtaa gaaaacaggg aaagcagcaa
     7081 tatcaggacg accgccgccg ccggcgaata aggcggcagg ttgtccgggc cgatatagcg
     7141 cataaaggcc aatatcggcc aatgccacag ataaagcgaa taggaaatca aaccggcggc
     7201 aacggtgatt ttccattgga aaaatttttt aagcgggtgt tcgtaatgat tgaaataaat
     7261 cagcgcggca acagccagac agggaatcaa agcggcgggc cctgggaaat aggcggtttg
     7321 ttccgaatag gcaaacaggc aggcggacag tatgcacgcg gaaaacaatg cgccggcggc
     7381 ggcacagcat ctgccgacgg cgagattccg ctgttgccgg taccgcatcc acacggcggt
     7441 cagcgatccg accagcattt cgcaggcgcg caggtggggc aggtaatatt tatcgaacgc
     7501 ggaaggcata aaggaagcgg caaggcttaa ggcgcacaat gcggcgagga agccgaactg
     7561 tacgcgcggg cttttgcggg cgacaagcag cagcaatatc ggaaagacaa agtaaaattg
     7621 ttcttcgacc gacaaagacc aaatgtgcag caggggcttt tcttcctgcg cgggatcgaa
     7681 ataatccttc ccccttgcaa aatacaggtt agaggcgaaa cccaaggcgg tcagcgcgga
     7741 tttccacaaa agaaagaaat catctttggt gaataaaaag aagccgcctg ccagcgttgc
     7801 cgccaatacg gcgaaaaatg cgggcagaat ccgcttgatg cggcggatat aaaatgcctt
     7861 cagggaaaac ctcccccccc ccccgacatt tcgcggtgaa gaatcgtcgt catcaaaaag
     7921 cctgaaatca caaagaatat atcgacaccg agaaacccgc ccggcagcca atccttttcg
     7981 atatggaaca cgatgacgga caagacggcg gcggcgcgca atgtgtcgat gtccgggcgg
     8041 tagggtaagg cttggctcat aatgttttta tagtggatta aatttaaacc agtacggcgt
     8101 tgcctcgcct tgccgtacta tctgtactgt ctgcggtttc gtcgccttgt cctgatttaa
     8161 agttaatcca ctatactcga aacgcggcgg cgcaaatgcc gtctgaaagg tcattttgta
     8221 cgggatattc ggaatgccgg acggattttt gcaacagcgg ggcaggcggt ttgttttgca
     8281 ggaatcgggg agggcaaatc ggaaatgcgg acgggatttt attttgatgc agccgcattc
     8341 cggcggtatg gaaacgccga aaaccaccaa aaccggcttc agacggcatt tctggcaaag
     8401 ccgtctgaaa acctgccgca tttgggttac acgttaaaca aaaagtgcat cacatcccca
     8461 tcctgcacga cgtattcctt accttccaca cgcattttgc cggcttcttt ggctttggct
     8521 tcgccgccga gcgagacaaa gtcgtcgtaa gaaatgactt gggcgcggat gaagccgcgt
     8581 tcgaagtcgg tgtgaatcac gccggcggct tgtggggctg tgtcgccttt gtgtatcgtc
     8641 cacgcgcgga cttctttgac gccggcagtg aagtaggttt gcagccccaa aaggtcgtaa
     8701 ccggcgcgga tcaggcggtt caggcccggt tcttccaagc ccatttcggc gaggaactcg
     8761 gctttttcac cgtcttccaa ctcggcgatt tcgctctcca tcgcggcgca cacggcgacg
     8821 acgggggcgt tttcttttgc cgccaattct ttcaggcggt cgaggtgcgg gttgttttca
     8881 aaaccgtctt cggaaacgtt gccgacgtac atggcaggtt tggcggtcag caggaacagc
     8941 ggtttgagca tcgcgcgttc ttccgcgtcc aaaccgaagg aacgcacggg tttgccttcg
     9001 tccagatgcg gcagcagttt tttgcacaaa tcgaccagct tttgcgcgtc tttgtcaccg
     9061 gaacgggcgc gtttttcttc gcggacgatg gctttttcca cacttgccaa gtcggcaagc
     9121 gccaactctg taccgatggt ttcgatgtcg gcaatcggat cgactttgcc ggaaacgtgg
     9181 acgatgttgt cgtcgtcaaa gcagcgcacg acattcacaa tcgcatcggt ttcgcggatg
     9241 ttggcaagga actggttgcc caagccctcg cctttgctcg cgcctgcaac caaaccggca
     9301 atatcgacaa attcgacgat ggcaggctgc attttttgcg gattgacgat ttttgccaat
     9361 tcggccatac gcggatcggg gacttcgacg atgccgacgt tgggttcgat ggtacagaaa
     9421 ggatagtttg ccgcttcgat acccgattgg gtcagcgcgt taaaaagggt ggatttgccg
     9481 acgttgggca aaccgacgat gccgcatttc aaactcatgt tttttcctga aaatagagaa
     9541 atttaacggc ggattatagc ataccgccgc ccgcgttccg aaaaatgccg tctgaaacgg
     9601 cttcagacag catccggttt caaaaaccgt tcagaacaag ccgtgaatca cgccgtgttc
     9661 gtccacatcg attttctcgg cggccggaac tttcggcaga cccggcatct tcatcatatt
     9721 gccgcacagc gcgacgatga agcccgcgcc ggcggaaacg gtgatgccgc gcacggcgat
     9781 gcggaagcct tcggggcagc ccaacagttt ggcgttgtca ctcaaagagt attgggtttt
     9841 cgccatgcag atcggcattt tgtccaagcc cagtttttcc agtgaagcga tttcggcaga
     9901 cgcttccgcg ctgaaatcaa catcttccgc gccgtacact ttttgggcaa tcgcacggat
     9961 tttgtctttg atgcccaact cgacatcgta ggcgaaaccg aagttattgg tttgactttc
    10021 aatggcgttg acgactttgc gcgccaaatc cgcgccgccc gcaccacctt tgccccacac
    10081 ttcggtcagg gaaacttcaa cgccgtattc ggcacaggct ttttcaatca tcgccaactc
    10141 ggcatcggag tcggacacga agcggttgag cgcaacgacg acgggcagtc cgaatacgtt
    10201 tttcaggttg gaaatgtgtt tcagcaggtt gggcaaacct ttttccaaag cgtctaaatt
    10261 ttcttcgccg aggttggcgc gttccacgcc gccgttatat ttcaacgcgc ggacagtcgc
    10321 cacgacaaca gccgcatcag gtttcaaacc ggcaaggcgg catttgatgt cgcagaattt
    10381 ttccgcgccc aagtccgcgc cgaagcctgc ttcggttacg gcgtaatcgg caaggtgttt
    10441 cgccagacgg gttgcggtta cggagttgca gccgtgggcg atgttggcga acgggccgcc
    10501 gtgtacgaag gcgggcgtgc cttcgatggt ttgcaccaag ttgggcttaa tcgcatcttt
    10561 aagcaatgcc gccatcgcgc cattcgcttt caaatctttg gcgtaaacgg ggctgccgtc
    10621 tttggcgtag gcgacaagga tgttgcccaa acgctctttc aaatcgctga tgtctttggc
    10681 aagacagaat accgccatca cttcggaagc aacggtaata tcgaaaccgt caggacgcat
    10741 cacgccgtca acaggcttgc ccatgccgtc gatgatgttg cgcaactggc ggtcgttcat
    10801 atcgaccacg cgccgccaca gcacgcgttt ggggtcgatg ttcaactcgt tgccttggta
    10861 gatatggttg tcgagcatcg cggcaagcag attatttgcc gcaccgatgg cgtgaaaatc
    10921 tccggtgaag tgcaggttga tgtcttccat cggcaaaact tgggcatagc cgccgcctgc
    10981 cgcgccgcct ttcacgccga acaccggccc taaagaaggt tcgcgcaggg caatcacggc
    11041 atctttgccg atgtggcgca acgcgtccgc caaaccgatg gttacggtgg ttttgccttc
    11101 gccggcagga gtcgggttga tggcggttac caaaatcagc ctgccttgtt tttgcggcag
    11161 tttaaacgct tcggcaggat tgattttggc tttgtaatga ccgtaaggct caatgttgtc
    11221 ggcgttcagg cccagcttgg cggcaatttc gccaatcggg cgcatggtgg aagactgggc
    11281 gatttcggca tcggttttga agctcatgat tttcctttag aaatgaggag ggacataccg
    11341 tctgaaagca tcaggcgaca aacaggtgga ttgaaaataa tatcaggcat attataacgt
    11401 tatccgcacc aaacccgcag tgaaattttt gacgctgcaa caaaaatacc gttcatattg
    11461 ttcacaatcc aaggagaaaa catgggcagc aacgcatggc tgttttgggc attggcatcg
    11521 gcaggcttcg cctcattgac cgccgttttc gccaaaatgg gcttgcaggg tatagattca
    11581 gatttcgcta ccttcatccg caccttggtc atcctcgccg ctttggtact gtttttgacc
    11641 tacaccggca aatggcaggg cgtgaacggc tttacggggc acaactggac attccttatc
    11701 ctatccggcc ttgctaccgg cgcatcttgg cttgcctact tcaaagccct gcaactgggt
    11761 aacgcctcgc aagtcgcccc cgtcgataaa ttcagcctgg ttctggtcgc gctgatggcg
    11821 gtggttttct tggacgaacg cccgaacacg caggaatgga taggcttggg gctggtaacg
    11881 gcgggcgtgt tggtgctggc gttgaaacgt taaaccgaat ccgccatacc gcctgaaacc
    11941 gggttttgac aatctgacgg gcggcggtct gaacaaagtt acccaataaa atccgacacc
    12001 gcgccgacgg caaaccgccg cgcttcagat ggcatttgtt ggacggaaag ccccgaacgc
    12061 cctattttca cgcctccacg aaaatatgcc ggacatccgg tcggaaccgt tcggcagccg
    12121 gctcggcaag ccaccgtttt atttcacttc caagcccctg caagggcttg agcctctttc
    12181 agacggcata ccgtgccgac atccagccac aagcccgtat gcttctgacc gctcacgcgg
    12241 ttttgccgca tttcgttccg caatacgggc gcgagtttcg ccacactgcc cgcttcgatt
    12301 ccgtcaaaca tttcaggacg gtaaataccc acgccgctaa atgtcaatcc gttgccgcca
    12361 tttacttccg gccgcacgct gccgtcgggc agcagggaaa aatcgccgtc aggatggtgc
    12421 gggggatttt ccaccagcca cagatgggcg gaaatgtgct cgggcaggga cgatgccgcc
    12481 tgaaacgcgg cggtaaaatc gatgtcggtc aacacgtcgc cgttgaccac caaaaacggg
    12541 gcatcgccca acagcggcaa tgcccgtgcg ataccgcctg cggtttccaa gccgccttca
    12601 ggttcgggcg aataggcgat gttcacgcca taagccgagc cgtcgcccaa agcctcttct
    12661 atctgccgac ccagccaagc gtggttgatg acgatttcgg taaaccctgc ctgcttcaga
    12721 cggcataggt gccaaccgat tagaggctta cccgccacat cgagcagcgg cttcggagtg
    12781 gtatcggtca aagggcgcat acgctcgccg cgtcctgccg ccagtatcat cgctttcata
    12841 tatatctgtc cgaatatcag tctaaagatc taaactgccg tctgaaataa agcagcgcgg
    12901 gacgtttgcc cacgcaattt ttaatttcgt cgagcctgac gtaaaacaca aaatgtgtgc
    12961 cgatttcatg ttggccgaca atatgcccgt gcagatgcgc caacgcgcct tctatttcag
    13021 gttgtccggt tttaccgtaa tgccaaatat gataggcaaa acgttcttcg ggtgaaagat
    13081 tggtcaatcc ggcaaaatgt tcggccacat cctgatgttc gtctgccaac gtattgatgc
    13141 acaggctgcc gttttccgac aggatcggaa tgattcgcgc actccggttg atgcacagca
    13201 tcacggtcgg cggctcgtcg gtaaccggcg cgaccgccgt cattgtaatg ccgtaacgcc
    13261 ctgccgcacc gtctgtcgtg atgacatgaa cgcctgccgc gcaagatgcc atcgcatcac
    13321 ggaacgaagt ttgaaaagtt ttctgcaaat ccgccatttt tcccctttaa actgtcccct
    13381 atataagaat gctgcacaca aggcaccccc catgtgcagc agttctgatt caaaaagccg
    13441 tcggtcggac atttccgcgc gttacggcgt attacgagtt caacgcatcc tcgattttgg
    13501 caagttctgc caacaggtct ttaagcagca gcattttctc gcggcccagc acttcctcga
    13561 tagcgtcgta acgctcgtcc acttcttcgc cgatttcctc atacagcttc tcgccctcgg
    13621 cagtcagctt cagaaaaaca cgtcgttggt cgttggaagg tttcaggcgg acaaccaaac
    13681 ccgctttttc aaggcgggtc aggataccgg tcaggctggg gcgcaaaatg cacgcctgat
    13741 tcgccaaatc ttgaaagtcc agcgtgccgt tttccgccaa aagacggata atccgccatt
    13801 gctgatcggt aatattcgcc tgattcagaa taggcctgaa ttgggtcatc agggcttccc
    13861 ttgcctgtat cagactgata ttgatagacg catgttttga ttgggtaggc attgttcaag
    13921 tctccaagtt atcgaaaatc aaactttcaa accgtcggga aagcctgtgg gcgtaatttt
    13981 tgatgcaacc gttatataac aaaacgaaca tatagcaaca atacgctata aaccgcatcg
    14041 gacgactggg tataaaagac tttaattccg ataatcctat ctaaaaatat tttaatagtt
    14101 atatcttagt ctattttccc tacaatctca acaaagtatt acatttactg tcgaaagaac
    14161 tttcttccag aaaaaacaaa agccgccgca tcagacgagc aaggtatatg ccgtttgatt
    14221 ctctacatag cgtaaagttt aacaaaaaca aaagttaacc gaaaacatcc gcctgaaaaa
    14281 ttcgtgcgcg caagccccaa taactgctga ttcccgtcgt ataatggacc attttcccat
    14341 ttttgaccaa aacgacggca ggcgttgcga caatccgcca agaccttgcc aaacccccgt
    14401 cctcatcgtt gacagtcgga aagcccaagc cgcgttttgc catatacgcc gccacttccg
    14461 ccgaactgcc ggaacgtacc gccacgccga cgaccggcac gccgtccgcc gccaaatcat
    14521 cgattatcgg cgactgataa cggcacacgc cgcaccagct tccccaaaaa tacaccagca
    14581 ccgtcctatc ccggctaaac tgtcccaaag tcagccgctg ccccgacagc agggtcaaag
    14641 gccgccctgc cgcgccggca ggttcgtcgg gcttgcgtac ccaatccaaa aacagcgaca
    14701 ccaataaaaa caccaatgcc gtctgaacgg caaatttgat gcccgaaagc agtttctttt
    14761 tcatacgctc tctcaaacgg tacgcccgcg caaccggcag gcaaacaaaa agccaagtct
    14821 caaaacttgg cttccggtta tctggtgggt cgtgagcgat tcgaacgctc gaccaacgga
    14881 ttaaaagtcc gctgctctac cgactgagct aacgacccga taagccgtgc attatacagc
    14941 accatcctac ctcgtcaagc aaattttaca ggcttaattg cagaccactg tttgcacggg
    15001 atattttgac aacggatttt cacaatccgc cgcataccgt gtaaaagttc gcacaaggaa
    15061 aagcaaaccg cccgaaatca atgtacactt tccgcccgtt tcccaaactg cacacagaaa
    15121 cacacattat gaacatacaa aacatccgca ccctcctcga caccgtcgcc gttccgaata
    15181 cggcacgcac gctcggcggc gaaaaggccg tccgttcggt cgaacagcgt tcagacggca
    15241 tccatatcgc cctgcatttc ggcttccctg tcgcgcacat cgccgccgcc gtcgccgatt
    15301 ccgtacaaga aatcctgatg cccgaaacag gcggcgcgca catccatctg tccatagaca
    15361 ccgaaatcgg cacacataaa gtccaacctg gcgttaccac catcaaaggt gtgaaaaaca
    15421 tcattgccgt cgcctccggc aaaggcggcg tgggcaaatc aacaaccacc gccaaccttg
    15481 ccgccgcaat ggcgcggatg ggcgcgcgcg tcggcgtgct cgatgccgac ctttacggcc
    15541 cgagccaacc gaccatgttg ggtgtggacg accgcaaacc cgatcagaaa aaccaaaaac
    15601 tcattcccgt cgaatcttca gacggcatac aggtcatgtc tatcggcttt ctcgtcgata
    15661 ccgaccaagc cgtcgtctgg cgcgggccga tggtcagcca agccttgcag caactgatgt
    15721 ttcaaagcga gtgggacgaa gtggactacc tgtttatcga cctgcccccc ggcacgggcg
    15781 acatccaact cacgctgtcc caacgcatcc ccgtaaccgg atcggtcatc gtaaccacgc
    15841 cgcaggacat cgccctgata gacgcgcgca aagccgtgga tatgttccga aaagtcaaca
    15901 ttcccatttt aggtgtattg gaaaatatgt ccgtccacat ctgctccaac tgcggacaca
    15961 gcgaagcact gttcggcaca gacggcggca aagacttggc ggcacgcctc aacgtccccc
    16021 tgctgggaca gcttccatta agcctgcccg tgcgcgaagc catggacgga ggggcgacgg
    16081 cgcaactgtt cgacgaacac cccgccatcg cccgaatcta caccgatgcc gcattccaaa
    16141 tcgccctggg cattgccgac aaaggcaaag acttcagcag ccggttccct aaaatcgtcg
    16201 tcgaataaag ccgcgtccga aaccgcaaca gcaatgccgt cccaagcccc gcgcctgccg
    16261 atatgccgtc attcccgcgc aggcgggaat ctagaacgtg gaatctaaga aaccgtgttg
    16321 taaaggcaga ccgatgccgt cattcccacg aaagtgggaa tctagaacgt aaaatctcaa
    16381 gaaaccgtta tacccgataa gtttctgcac cgacaggtct ggattcccgc ctgcgcggga
    16441 atgacggttc gggtatttct gacgatttag gcattcccga caaggtggat tttcaaggtg
    16501 ttgtataggg tgtaggagga ttcgtaaaag gtgagttata gggtgggctt cagcccaccg
    16561 attccaacga ttccaccaat cctacaccgt tcccatagac tcaaatcaac acagaaactt
    16621 atgcgccgtc attcccgcgc aggcgggaat ctagacctta gaacaacagc aatattcaaa
    16681 gattataaaa gacccgtcat tcccgcgtag gcgggaatct agaccattgg acagcggcaa
    16741 tattcaaaga ttatctgaaa gtccgagatt ctagattccc actttcgtgg gaatgacgcg
    16801 gtgcaggttt ccgtgcggat ggattcgtca ttcccgcgca ggcgggaatc cagaccttgg
    16861 gataacggca atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcta
    16921 gaccttagaa caacagtaat attcaaagat tataaaagac ccgtcattcc cgcgcaggcg
    16981 ggaatctaga acgtaaaatc taaagaaacc gtgttgtaac ggcagaccga tgccgtcatt
    17041 cccgcgcagg cgggaatcca gactgtcggg catctgcagc ggtttgctaa aaaacgcttt
    17101 accgtgatca gtgtgcaaag ttaaaatggg gaggtaagct ttccaaccag caattcggcg
    17161 ggcgcgggat cgggcggttt gccgaatccc gccgtttgcg gcgcgcctgc cgccgacggt
    17221 atcccgcgaa gcaagattta agggataaaa tatgttccaa cacgcagggc ggcacataag
    17281 gcgccgccct gattcggaag ggcttgcacc cctcccggac aaagcctgat cctgccgccc
    17341 cgaaggacgg atgcccgaag ggcggggggt ttgaccgaaa aggaaatacg atgaataaaa
    17401 ctttaaaaag gcgggttttc cgccataccg cgctttatgc cgccatcttg atgttttccc
    17461 ataccggcgg gggggggcgc aggcggaaac ccataactac gctattgtca tgaacgaacg
    17521 aaatcagctg caggtaaagc aggagggttc atattcaacc ttaagggaaa aagacaggaa
    17581 acgcaaattt atctatacga gccagagaaa cggggcgggc caacaaaaca attttatctc
    17641 attcgacaat accgataccc ttgtttccca acaaagcggt actgccgttt ttggcacagc
    17701 cacctacctg ccgccctacg gcaaggtttc cggttttgat gccgacgggc tgcaaaagcg
    17761 caacaatgcc gtcgattgga ttcataccac ccaggccggg ctggcaggct acgcctacac
    17821 cggcgtcata tgcagaagca accaatgccc ccaacttgtc tatgagacca aattttcctt
    17881 cgacggcatc ggtttggtaa aaaatgcggg cagcctggat aggcacccgg acccaagccg
    17941 cgaaaattcg cccatttaca aattgaaaga tcatccatgg ttgggcgtat ctttcaattt
    18001 gggcagcgag ggtaccgcca aagatggcaa ggtatccaac aaattggtat cttcttttga
    18061 tgaaaacaat agtaatagta atcaaaacct cgtctatacg acaaaaggcc accatatttc
    18121 ccttggcgac tggcagcgcg aacataccgc catggcctat tatctgaacg ccaaactgca
    18181 cctgctggac aaaaaaggga ttaaagatat cgcccaaggc aaaacagtgg atttgggcac
    18241 cttgagaccg cgcgtcgaga cgacaggacg aagctggcta gatttttggg ctacgtggaa
    18301 gattgaagat aaagggaaca ttacagtccg cctcggcctg ccggaagtca aagcaggccg
    18361 ctgcatcaac aaaccgaacc ccaatcccaa agcccaagcc ctttcgccgg cactgactgc
    18421 ccccgcgctg tggttcggac ctgtgcaaaa tggcaaggtg cagatgtatt ccgcttcggt
    18481 ttccacctac cccgacagtt cgagcagcca aattttcctg caaaaccttt cccgcaagga
    18541 tgacacaagc aaaccgggcc gctattccct caaacccttg agtacgtcgg agattaaaag
    18601 taaagagccg actttcacag ggcggcaaac cgtcatccga ttggatggcg gcgtacagca
    18661 gattaaactt caaggcaatg aggtcactag ttttaatgta aacaacggca acaacacttt
    18721 cggcattgtt aaggacttgg gcgtcgatcc tgatgccagc gagtggaaaa aagtgctgct
    18781 gccttggacg gttcgtgctt ccaatgatga caatcaattt aaaacaatta accaacaact
    18841 taaccaacaa aaaatccaat acagccaaag ataccgcatc cgcgacaacg gcaatcgcga
    18901 tttgggcgac atcgtcaaca gccccatcgt ggcggtcggc gagtatttgg ctacttccgc
    18961 caacgacggg atggtgcata tcttcaaaca aagcggcggg gacaagcgca gctacaatct
    19021 gaagctcagc tacatccccg gcacgatgcc gcgccaatat tttgataacg acacttccgc
    19081 tctcaaagac tccaccctcg ccaaagagct gcgcaccttt gccgaaaaag gctatgtggg
    19141 cgaccgctac ggcgtggacg gcggctttgt cttgcgccgc attacagatg accaagacaa
    19201 gcaaaaacat ttctttatgt ttggtgcgat gggcctgggc ggcagaggcg cgtatgcctt
    19261 ggatttgacc aaagccgaca gcaataaccc gaccgccgtt tccctgtttg atgtcaaaaa
    19321 tggcaataat ggcaagaata gcaataatag caataatagc gtgcaattag gctacaccgt
    19381 cggcacgccg caaatcggca aaacccacaa cggcaaatac gccgccttcc tcgcctccgg
    19441 ttatgcgact aaagacatta acaacggcga caataaaacc gcgctgtatg tgtatgattt
    19501 ggaaagcaac aacggtacgc cgattgcaaa aatcgaagta cccaacggca agggcgggct
    19561 ttcgtccccc acgctggtgg ataaagattt ggacggcacg gttgatatcg cctatgccgg
    19621 cgatcgcggc ggcagtatgt accgctttga tttaagcggc aacaacccga ccagttggtc
    19681 tgcacgcgcc attttcagcg gcaacaaacc gattacttcc gcgcccgcca tttcccaact
    19741 gaaagacaaa cgcgtggtta tcttcggcac gggcagcgat ttgagtgaag atgatgtgga
    19801 caatacggac gaacaatata tttacggtat ctttgacgac gatacggtgg cgaataacgt
    19861 aaatgtaaaa ctcagcggtt tgggaggcgg gctgctcgag caaaagctta gtgaggaaaa
    19921 taaaacctta ttcctgaccg attataagcg atccgacggc tcgggcgaca agggctgggt
    19981 agtgaaattg aaggacggac agcgcgttac cgtcaaaccg accgtggtat tgcgtaccgc
    20041 ctttgtaacc atccataaat atacgggtac ggacaaatgc ggcgcggaaa ccgccatttt
    20101 gggcatcaat accgccgacg gcggcaagct gaccaagaaa agcgcgcgcc cgattgtgcc
    20161 ggaagccaat acgaaggtcg cgcaatattc cggccataag caaaccgcca aaggcaaatc
    20221 catccctata ggttgtatgg aaaaaggcaa tgaaaccgtc tgcccgaacg gatatgttta
    20281 cgacaaaccg gttaatgtgc gttatctgga tgaaaagaaa acagacggat tttcaacaac
    20341 ggcagacggc gatgcgggcg gcagcggaac attcaaagag ggtaaaaaac ccgcccgcaa
    20401 taaccggtgc ttctccggaa aaggtgtgcg caccctgctg atgaacgatt tggacagctt
    20461 ggacattacc ggcccgacgt gcggtatgaa acgaatcagc tggcgtgaag tcttctactg
    20521 atttgcacgc gaaaatgccg tccgaaaggt tttcggacgg cattttttgc gtttttcggg
    20581 aggggcgggt tcgtaaaagg cgggctatag ggtaggcttc atctcgccaa tctcactgaa
    20641 tccatcaatt tccacaattc aattaaatac cgtcaaaccg atgccgtcat tcccgcgcag
    20701 gcgggaatct agaccttaga acaacagtaa tattcaaagg ttagctgaag ctttagagat
    20761 tctggattcc cactttcgtg ggaatgacgg gatttgagat tgcgacattt atcgggagca
    20821 acagaaaccg ctctgccgtc attcccgcgc aggcgggaat ctagacctta gaacaacagc
    20881 aatattcaaa gattatctga aagtccggga ttctggattc ccactt
//
LOCUS       DAWFIT010000030        20672 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFIT010000030 DAWFIT010000000
VERSION     DAWFIT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.030"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(110..538)
                     /locus_tag="WGQ85_001034"
     CDS             complement(110..538)
                     /locus_tag="WGQ85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0750473.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WGQ85_001035"
     CDS             complement(603..2351)
                     /locus_tag="WGQ85_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0750474.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WGQ85_001036"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WGQ85_001036"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0750475.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WGQ85_001037"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WGQ85_001037"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0750476.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WGQ85_001038"
     CDS             4754..4924
                     /locus_tag="WGQ85_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0750477.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WGQ85_001039"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WGQ85_001039"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0750478.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WGQ85_001040"
     CDS             6085..6279
                     /locus_tag="WGQ85_001040"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0750479.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WGQ85_001041"
     CDS             6481..7890
                     /locus_tag="WGQ85_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0750480.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="WGQ85_001042"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="WGQ85_001042"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0750481.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEYPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILVEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGQ85_001043"
     CDS             complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGQ85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0750482.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13173..13385
                     /locus_tag="WGQ85_001044"
     CDS             13173..13385
                     /locus_tag="WGQ85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750483.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(13664..14296)
                     /gene="mtrR"
                     /locus_tag="WGQ85_001045"
     CDS             complement(13664..14296)
                     /gene="mtrR"
                     /locus_tag="WGQ85_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0750484.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            14704..15942
                     /gene="mtrC"
                     /locus_tag="WGQ85_001046"
     CDS             14704..15942
                     /gene="mtrC"
                     /locus_tag="WGQ85_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0750485.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            15954..19157
                     /gene="mtrD"
                     /locus_tag="WGQ85_001047"
     CDS             15954..19157
                     /gene="mtrD"
                     /locus_tag="WGQ85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0750486.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19212..20615
                     /gene="mtrE"
                     /locus_tag="WGQ85_001048"
     CDS             19212..20615
                     /gene="mtrE"
                     /locus_tag="WGQ85_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0750487.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPNDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
BASE COUNT         5018 a         5088 c         5576 g         4990 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgt tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaccgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat caggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag
     7261 gttaaggaag tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac
     7561 ccgttcggca aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg
     7621 agcgtgttgg cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa
     7741 atcaagctgc ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc
     7801 ccgaccgcgc tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctaggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctt gtctatcgtc cgcagtggat agacgcgtgg cagcagggca
     8461 ggatactcag tttgggcgac gatgaaatct ggcagtccaa actgtggcgt tatcttgatg
     8521 acggcaggca gagcgcgccg caccgtgttg cgttgtggga aaagctgttg gaatctttga
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaac attgcgatgt gttcgtgttt gcactcaatc
     8701 cgagtgggat gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc ggtacggatg acggttcgat acgtatcgtc tccgcgcaca
     9001 gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaatatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa tatcgaatcc tatacgcctt
     9121 ttatcgaggc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cggacgtgaa aataagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgacatg gttgccgatt
     9361 tgaacgtccg ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaatccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg ttcggacgtt
     9541 ttgccgcctt cctcgaaacc ctgtcggata ttgtacggat atggcggcaa cctgcgacgg
     9601 ttgatgaatg ggtaggacgt tgccgaggtt tgcttgaaac attgttccaa gccgaagccg
     9661 atgaccaaaa ggcagtccaa aacctcgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccac atccgccgct
     9781 ttctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccc tttaaagtca tctgtctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgcgcccg ccgcgacgac gaccgctacc tcttcctcga agccctcatc agcgcgcgtg
    10021 aaatcctgta cctgtcctac atcgggcgcg acatccgcaa agacgaagaa ctggctccgt
    10081 cttctttgtt gggcgaactc atcgataccg ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaacctgata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc gccgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgatcaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gcttctggcg caaccctgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagcc gcaacacgcc gatcaaatcg ccgaaatcta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag caggacttcc aaaccgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag tcccaaactt cctccgctgc cctacgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttttgt tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 tggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaaaacctc gcttgccacc gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc taccacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc gctcgaacag cccctgtttg aaaacctcgt ccgtctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaaag gaagaggccg gagaagcctg acgggatgga aaggcaaatg
    11221 ccgtctgaaa acagcaaaaa ggttttcaga cggcattttg tttatcgtgt ttaaggtgcg
    11281 ggatagaatg tgggcgcgcc gggaccgacg ggcaggccca aaacaaatac ccaaatgcag
    11341 aataaggcaa tccacgcaat caagaagaaa gcggaatacg gcaacatcat agaaatcagc
    11401 gtacccacgc ccgcatcttt tttgtatttg atcaccgtcg ccataatcag cccgaaataa
    11461 ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc ggcttgaatg
    11521 acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc agttaccgcc
    11581 cattgcgcgg aggcggagcc tatcatcaga ttgataaaag cacaaattaa aataaaaccg
    11641 ataaacaaca cgctgccgcc caagccgact tcttttaaga acgtcgcccc tttaacggca
    11701 atatattgcc caatattcgt ccaattaaaa aatgcgacaa actgtgcggc aaaaaagatg
    11761 atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac gacttcctgt
    11821 tcgccgcgca aacttcgggt tacccggcca taaacaatgc ccggcagtgc aaacaacaag
    11881 aaaataaaaa caacaatcga ttttaaaaac ggcgaaccgg aaaccaatcc tgtttcagga
    11941 tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga taaggcaaca
    12001 aacaccacgc cagcccaaat taatccttta tattccaaag gcgtgatttc attggaatgc
    12061 cgaaagtctt tttcttcttg tgacaaatct gattgataag ggcccaattg cggttcgacg
    12121 attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggc taccataaaa
    12181 aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc cgcctgttgg
    12241 gtgatgcctg ccaagagcgg atcgattgtg cctaagaaca gattggccga ataaccgccc
    12301 gaaacgccgg cgaaagccgc agccagaccg gcaagcggat ggcggccgag ggaatgaaag
    12361 atgatggcgg acaaagggat taggacgaca tagcccaatt cagaagcggt attagataaa
    12421 atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gagattttgt gagcaataag
    12481 cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa taaagaaacc
    12541 aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt caggattttg
    12601 atcaaaccgt cagcatcgag caggctgaca acgtgaatca aaccgtcatc ggcacgtcct
    12661 ttcgcaccaa cagggcgcgg atcggggacg gatagtccga aatacgcacc ggcggcagag
    12721 gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg caacatattg
    12781 cccagccatt cgactgtgcg taaaaatcgt ccgtcccgtt gcgtatcggt ttgactcatc
    12841 tgcttctcct taaaaaaaat atattgagtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgtatatg tatataaccg taatagatta aaacagaaaa
    12961 tgcccacatc gttattctca taaaggcggt aatccgaaaa cttgacgtta aagccttatc
    13021 aggaatgact ggaactcaac aaagcggttt cccgctttcg cggaaatggc gggattttcg
    13081 gtttttgttt gcaaaaatgc cggagaaaaa tgcagtttac attttgaatg gtattgttat
    13141 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaattatgg
    13201 acaaatatga tccatggcat gaagatgatt ttgagtcgta tgaagacatt gccaaagatg
    13261 tatcgttgat gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13321 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13381 tttagtgaat atgagacctt tgcaaaaata gtctgttaac gaaatttgac gcataaaaat
    13441 gcgccaaaaa attttcaatt gcctaaaacc ttcctaatat tgagcaaaaa gtaggaaaaa
    13501 tcagaaaagt tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta
    13561 ttgcaaaggt ctcaatatgt atttttagaa gaaggctttg tgtcattttg atgccgtctg
    13621 aaagggtttg ttcgtttcag acggcattgt taccaaggct tgattatttc cggcgcaggt
    13681 cgggatggtt ttccaagttg tccatcatta tcccgatgat gcgcggggcg gttttgccca
    13741 aatcgaaacg ttcgcaggaa gagaaccacc gccaaatcag cccgtccaag gttgatttga
    13801 tgaagatgac cgctgtttcc ttgtccaaat cgtcagccaa atcctgattt tccaccgctt
    13861 cggtcaaaac ggcggtaatt ttctcgcgcc agattgcctg atgcttgcgg gcaatggcga
    13921 taacggcggc gttttgttcc gtgtgttcgc attttaaaaa caggatgttg tggaatttgt
    13981 agtagatgtc gttgctttgc agccgctcga aaaagtgcag cagcgtgtgg cggaataccg
    14041 cccaagaccc tccttcggca tcttcggcat cttgcgcgat gcagttttcg atgtcgtcgc
    14101 agatacgttg gaacagcgcg tcgaacaagt cttccttatt tttgaaatgc caatagagcg
    14161 cgccgcgcgt tacgccggcg gcttgggcga tttcgttgag cgaggtgcgc gcaatccctt
    14221 tgcggtaaaa ggtttccaag gcggcaagca tcaggtgttc tttggttttc aaggcttcgg
    14281 ttttggtttt tctcataatg gcgttttcgt ttcgggtcgg tttgatgagg gcggattata
    14341 aaaaagactt tgtaaccatg caatcgtgta tgtataatga aacccatgaa attgagacta
    14401 catctcaact ttgaaaacag gcataacctg ctttgcaacc cgttcgaaca ttcgggtatc
    14461 aaagcaggcg gtttttatat cctgaaatat agtggattaa caaaaatcag gacaaggcga
    14521 cgaagccgca gacagtacag acagatagta cggaaccgat tcacttggtg cttgagcacc
    14581 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
    14641 actataagaa tgcctgttga ttggcaaaac cctttacatc aaaccagaca gataggataa
    14701 ataatggctt tttatgcttt taaggcgatg cgtgcggccg cgttggctgc cgccgttgca
    14761 ttggtactgt cgtcttgcgg taaaggcgga gacgcggcgc agggcgggca gcctgctggt
    14821 cgggaagcac cggctcccgt cgtcggtgtc gtaaccgtcc atccgcaaac cgtcgcattg
    14881 accgtcgagt tgccggggcg tttggaatcg ctgcgtaccg ccgatgtccg cgcccaagtc
    14941 ggcggcatca tccaaaaacg cctgttccaa gaaggcagtt atgtccgtgc cggacagccg
    15001 ctgtatcaga tcgacagttc cacttatgaa gcaggtctgg aaagcgcacg cgcgcaactg
    15061 gcaacggctc aggcaacgct tgccaaagcg gatgcggatt tggcgcggta caagcctttg
    15121 gttgccgccg aagccgtcag ccggcaggaa tacgatgctg cggtaacggc gaaacgttct
    15181 gccgaggcag gtgtcaaagc agcacaggcg gcaatcaaat ctgccggcat taatctgaac
    15241 cgttcgcgca ttaccgcgcc gatttccggc tttatcggtc agtccaaagt ttccgaaggt
    15301 acgctgttga atgcgggcga tacgaccgtg ctggcaacca tccgccaaac caatccgatg
    15361 tatgtgaacg ttacccagtc tgcatccgaa gtgatgaaat tgcgccgtca gatagccgaa
    15421 ggcaaactgc tggcggcgga tggtgtgatt gcggtcggca tcaaatttga cgacggcaca
    15481 gtttaccctg aaaaaggccg cctgctgttt gccgatccga ccgtcaacga atcgaccggt
    15541 cagattaccc tgcgcgccgc cgtaccgaac gatcagaata tcctgatgcc cggcctgtat
    15601 gtgcgcgtgc tgatggacca agtggcggtg gataacgcat tcatcgtgcc gcagcaggcg
    15661 gtaacgcgcg gtgcgaaaga taccgtgatg attgtgaacg cccaaggcgg tatggaaccc
    15721 cgcgaggtaa cggtcgcgca acagcagggt acgaattgga ttgttacgtc gggtctgaag
    15781 gacggggaca aggtggttgt ggaaggcatc agtatcgccg gtataacggg tgcgaaaaag
    15841 gtaacgccca aagaatgggc gccgtctgaa aaccaagccg ccgcgcctca atccggcgtt
    15901 cagacggcat ctgaagccaa aactgcttct gaagcggaat aaggaaggca tcgatggcta
    15961 aattttttat cgaccgcccc attttcgcgt gggttatttc gattttcatt attgcggcgg
    16021 gtattttcgg cattaaaagc ctgcctgttt cgcaatatcc gtctgtcgcc gccccgacca
    16081 tcaccctgag ggccacttat ccgggcgcgt ccgcgcaggt aatggaagac agcgtgcttt
    16141 ccgtgatcga gcggaatatg aacggcgtgg aaggtttgga ttatctgtcc acttctgccg
    16201 attcgagcgg cagcggcagc gtgagcctga cctttacgcc cgataccgac gagaatctgg
    16261 cgcaggtgga agtgcagaac aagctttccg aagtattgag cacgctgccg gcaaccgtcc
    16321 agcaatacgg cgtaaccgta tccaaggcgc gttccaattt cctgatgatt gtgatgcttt
    16381 cgtcggatgt gcagtcaacc gaagagatga acgactacgc gcagcgtaat atcgttcccg
    16441 agttgcagcg tatcgaaggc gtggggcagg tacgcctgtt cggcgcgcaa cgcgcgatgc
    16501 ggatttgggt tgatcctaag aaactgcaaa actacaattt gtcgtttgcc gatatcggca
    16561 gtgcgctgtc cgcccagaac gtccagattt cagcgggttc tatcggttcg cttcccgccg
    16621 ttcgcggaca gacggttacg gctaccgtaa cggcgcaagg gcagttgggt acggcagaag
    16681 aattcggcaa cgtcatcctc cgcgccaata ccgacggttc taatgtttac ctgaaggatg
    16741 tggcaagggt cggactgggt atggaagact attcttcctc aacccgtctg aacggtgtaa
    16801 ataccaccgg tatggcggtg atgctgtcca acagcggcaa tgcgatggcg acggcaaagg
    16861 cggttaaaga acgcatggcg acgttggaaa aatactttcc tcagggtatg agctggaaaa
    16921 ccccttacga tacttccaaa ttcgtcgaaa tttcgattga aaaagtgatt catactttaa
    16981 tcgaagcgat ggtgctggtg tttgtcgtaa tgtatctctt cctgcaaaac atccgttata
    17041 cgctgattcc gaccatcgtc gtaccgattt cgctgttggg cggtttcgcc ttcatctctt
    17101 atatgggcat gtcgattaac gtactgacca tgtttgcgat ggttttggtc atcggcatcg
    17161 tggtcgatga cgcgattgtg gtggttgaaa acgtcgagcg cattatggcg ggtgaaggct
    17221 tgccgcccaa agaagcgacc aaaaaagcga tgggtcagat ttcgggcgcg gtcatcggta
    17281 ttaccgccgt tctgatttcc gtgttcgtac cgttggcgat gttcagcggg gcgacgggca
    17341 atatttacaa acagtttgcc ctgacgatgg cgtcatcaat cgcattctcc gccttccttg
    17401 cgctgaccct tacccctgct ttgtgtgcca caatgttgaa gacaatcccg aaagggcatc
    17461 acgaagagaa aaaaggtttc ttcggctggt ttaacaagaa attcaacagt tggacgcacg
    17521 gttacgaagg ccgggttgcc aaagtgctgc gtaagacttt ccgcatgatg gttgtctata
    17581 tcggcttggc ggttgtgggc gtgttcctgt ttatgcgcct gccgacttca ttcctgccga
    17641 ccgaagacca aggcttcgtc atggtcagcg tgcaactgcc tgcaggagcg acccaagagc
    17701 gcaccaatgc gactttggcg caagttaccc aactggcgaa aagcattcct gaaatagaaa
    17761 acatcattac cgtttccggc ttcagctttt cgggcagcgg tcagaatatg gcgatgggtt
    17821 ttgccatatt gaaagattgg aacgagcgta ccgcgcccgg cagcgatgcc gttgcgattg
    17881 ccggcaagct gacgggtatg atgatgggga cgcttaaaga cggttttggc atcgccgtcg
    17941 tcccgcctcc gattctggag ttgggcaatg gttcgggcct gagcatcaac ctgcaagacc
    18001 gcaataatac cggccatacc gcattgctgg cgaagcgcaa cgagttgatt cagaaaatgc
    18061 gtgccagcgg tttgtttgac cccagcaccg tccgtgctgg cggtttggaa gactcgccgc
    18121 agttgaaaat cgacatcaac cgtgccgccg cggcggcgca aggcatttcg tttgccgaca
    18181 tccgcaccgc attggcaagt gcgctgagtt cgtcttatgt cagcgacttc ccgaaccaag
    18241 gtcgtctgca acgcgtgatg gtgcaggccg acgaggatgc ccgtatgcag cctgccgata
    18301 ttttgaacct gaccgtgccg aacaaatccg gtgtcgccgt accgctttcc accattgcta
    18361 ctgtttcttg ggaaaacggt acggaacaga gcgtacgctt caacggttat ccttcgatga
    18421 agctgtccgc ttcgcctgca accggcgttt ccaccgggca ggctatggcg gcggttcaaa
    18481 aaatggttga cgaattgggc ggcggttaca gcttcgaatg gggcggacag tcgcgcgaag
    18541 aggcaaaagg cggctcgcaa accctgattt tgtacggttt ggcggttgca gccgtatttt
    18601 tggtacttgc cgcgctttat gaaagctggt cgattccgct ggcggtcatc cttgtgattc
    18661 cgttgggttt gatcggtgcg gctgcgggcg taaccgggcg caacctgttt gaaggactgt
    18721 tgggcagcgt tccctcgttc gccaacgaca tctactttca agtcggtttc gttaccgtga
    18781 tgggtttgag tgcgaaaaac gcgattttga ttatcgagtt tgccaaagac cttcaagcgc
    18841 aagggaaaag cgcggtcgaa gccgctttgg aagccgcccg cctgcgtttc cgtccgatta
    18901 tcatgacctc gttcgccttt attttgggcg tggttcccct gtatattgcc ggcggtgcaa
    18961 gttctgccag ccagcgcgcc attggtacaa ccgtattctg ggggatgctc atcggcacgc
    19021 tcttgtccgt gttccttgtt ccgcttttct atgtggtggt gcgcaaattc ttcaaagaaa
    19081 ctgcacacga acacgaaatg gcagtaaaac acgccgccga agcgggcatt accggttcgg
    19141 acgacagcca acattaagca accatgccgt ctgaacgccc acgggttttc agacggcatc
    19201 aggacttttt tatggatact acattgaaaa ctaccttgac ttctgttgca gcagccttcg
    19261 cattatccgc ctgcaccatg attccccaat acgagcagcc caaagtcgaa gttgccgaaa
    19321 cgtttaaaaa cgataccgcc gacagcggca tccgcgccgt cgatttgggt tggcacgact
    19381 attttgccga cccgcgcctg caaaagctga tcgacatcgc actcgagcgc aataccagtt
    19441 tgcgtaccgc cgtattgaac agcgaaatct accgcaaaca atacatgatt gaacgcaaca
    19501 accttttgcc cacgcttgcc gccaatgcga acggctcgcg ccaaggcagc ttgagcggcg
    19561 gcaatgtcag cagcagctac aatgtcggac tgggtgcggc atcttacgaa ctcgacctgt
    19621 tcggacgcgt ccgcagcagc agcgaagcag cactgcaagg ctatttcgcc agcaccgcca
    19681 accgcgatgc ggcacatttg agcctgattg ccaccgttgc caaagcctat ttcaacgaac
    19741 gttatgccga agaagcgatg tctttggcgc agcgtgtttt gaaaacgcgc gaggaaacct
    19801 acaagctgtc cgaattgcgt tacaaggcag gcgtgatttc cgccgtcgcc ttacgccagc
    19861 aggaagccct catcgaatcc gccaaagccg attatgccca tgccgcgcgc agccgcgagc
    19921 aggcgcgcaa tgccttggca accttgatta accaaccgat acccaacgac ctgcccgccg
    19981 gtttgccgtt ggacaagcag ttttttgttg aaaaactgcc ggccggtttg agttccgaag
    20041 tattgctcga ccgtcccgac atacgcgccg ccgaacacgc gctcaaacag gcaaacgcca
    20101 atatcggtgc ggcacgcgcc gcctttttcc catccatccg cctgaccgga accgtcggta
    20161 cgggttctgc cgaattgggt gggttgttca aaagcggcac gggcgtttgg tcgttcgcgc
    20221 cgtctattac cctgccgatt tttacctggg gtacgaacaa ggcgaacctt gatgtagcca
    20281 agctgcgcca acaggcacaa atcgttgcct atgaatccgc cgtccaatcc gcatttcaag
    20341 acgtggcaaa cgcattggcg gcgcgcgagc agctggataa agcctatgac gctttaagca
    20401 aacaaagccg cgcctctaaa gaagcgttgc gcttggtcgg cctgcgttac aagcacggcg
    20461 tatccggcgc gctcgacttg ctcgatgcgg aacgcagcag ctatgcggcg gagggtgcgg
    20521 ctttgtcggc acaactgacc cgcgccgaaa accttgccga tttgtacaag gcactcggcg
    20581 gcggattgaa acgggatacc caaaccgaca aataaccggt cgggcaataa aatgccgtct
    20641 gaaacgataa ggtttcagac ggcatttctt tc
//
LOCUS       DAWFIT010000031        20337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFIT010000031 DAWFIT010000000
VERSION     DAWFIT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.031"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGQ85_001049"
     CDS             complement(700..1284)
                     /gene="rnhB"
                     /locus_tag="WGQ85_001049"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0750488.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDTEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGQ85_001050"
     CDS             complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WGQ85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0750489.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRIFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDDIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAR"
     gene            complement(3572..4999)
                     /locus_tag="WGQ85_001051"
     CDS             complement(3572..4999)
                     /locus_tag="WGQ85_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0750490.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5159..6148
                     /gene="pdxA"
                     /locus_tag="WGQ85_001052"
     CDS             5159..6148
                     /gene="pdxA"
                     /locus_tag="WGQ85_001052"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0750491.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPTAEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKDLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGHL
                     GHEETDTIIPALKNLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(7032..9791)
                     /locus_tag="WGQ85_001053"
     CDS             complement(7032..9791)
                     /locus_tag="WGQ85_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0750492.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENSKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTSGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            10285..11277
                     /locus_tag="WGQ85_001054"
     CDS             10285..11277
                     /locus_tag="WGQ85_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0750493.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPAREFDIVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11325..12497
                     /gene="lpxB"
                     /locus_tag="WGQ85_001055"
     CDS             11325..12497
                     /gene="lpxB"
                     /locus_tag="WGQ85_001055"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0750494.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            12505..12751
                     /locus_tag="WGQ85_001056"
                     /pseudo
     CDS             12505..12751
                     /locus_tag="WGQ85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12753..13163
                     /locus_tag="WGQ85_001057"
     CDS             12753..13163
                     /locus_tag="WGQ85_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750495.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQESFN"
     gene            complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGQ85_001058"
     CDS             complement(13277..14086)
                     /gene="dapB"
                     /locus_tag="WGQ85_001058"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0750496.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(14096..14473)
                     /locus_tag="WGQ85_001059"
     CDS             complement(14096..14473)
                     /locus_tag="WGQ85_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0750497.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            14692..15126
                     /gene="fur"
                     /locus_tag="WGQ85_001060"
     CDS             14692..15126
                     /gene="fur"
                     /locus_tag="WGQ85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0750498.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            15196..15921
                     /locus_tag="WGQ85_001061"
     CDS             15196..15921
                     /locus_tag="WGQ85_001061"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0750499.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGQ85_001062"
     CDS             complement(16073..17104)
                     /gene="gap"
                     /locus_tag="WGQ85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0750500.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(17305..18144)
                     /locus_tag="WGQ85_001063"
     CDS             complement(17305..18144)
                     /locus_tag="WGQ85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0750501.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18293..20269
                     /locus_tag="WGQ85_001064"
     CDS             18293..20269
                     /locus_tag="WGQ85_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0750502.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVAKNA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
BASE COUNT         4006 a         4906 c         6359 g         5066 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaaaacggaa
      541 accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt ttttttttga
      601 ttttctattt ttcgcaggaa tgacggtttg ggttctttct ctttgaagtt gcgatgctga
      661 aaatgccgtc tgaagttcag acggcatttt tgtgccggtt taaaacaagg cctgctgcgc
      721 gagcaggttt ctgacggggg cgaagtctcg gcggtgttcg ggcagcacgc cgtattgttt
      781 gagggcttcc agatgctgct tcgtgccgta acctttgtgt ttgtcgaaac cgtattgggg
      841 atggcgttgc gccagtgcgt acatttctgt atcgcgtgcg gtctttgcca aaacggatgc
      901 ggcggagatt tcgatgattt tgctgtcgcc tttgacgacg gcttcggcag ggatgttcaa
      961 atgttcggga atgcggttgc cgtcgatgaa tattctttcg ggacgcacag ccaaaccgtc
     1021 aacggcgcgt ttcatcgcga gcatggtggc gtgcaggatg ttgaggctgg cgatttcttc
     1081 gggcgtggcg gcggcgacgt gccacgcggc cgcctgattt tttatcattt cggcaagcgc
     1141 gtcgcgtttt ttctcgctga gttttttgga gtcggtcagt ccgggcaggt cgaatgtttc
     1201 cggaaggatg acggcggcgg caaacacgct gccgactaaa ggtccgcgtc ctgcctcgtc
     1261 cacgccggcg gtcagtatgt gcatgatgtt tcctgtcggg atggtacgat gccgtctgaa
     1321 aagggtttca gacggcatcg cgccgatgtg tttatctcgc gtctttaaat ccgcgcttca
     1381 aatgcaccat cagcaatgcc actgccgcag gggttacgcc ggaaatgcgg cttgcttgtc
     1441 cgacggtttc gggtttgtgc tgattgagct tttgctgcac ttctgccgac aagcctttga
     1501 ctttgccgta atcgatatcg tcgggcagct ttaaggtttc gatgtcgcgg cggctgtcga
     1561 tttcttcgtt ttggcggtcg atatagcctt ggtatttgac ttggatttcg acttgttcga
     1621 tgacttcggc ggagaggttt tcagacggca tcgcgccttc gagcgtcatc agcgcggcgt
     1681 agtcgaggtt cgggcggcgt aacaaatcgt gcaggttggc ctcgcggctg agtttttgtc
     1741 cgaacacgcg gatttgttcg tcttcggcga gtttttgcgg tgtgtaccac gttgttttca
     1801 agcgttggat ttcgcgttcg acggcttcgc gtttttcgtt gaaaatgcgc cattgcgctt
     1861 cacccaccaa gccgattttg tagccgtctt cggtcaggcg catgtcggcg ttgtcttccc
     1921 tgagttgcag gcggtattcg gcgcggctgg tgaacatccg gtaaggttcg ttcacgcctt
     1981 tggtgatgag gtcgtccacc aatacgccga gataggcttg ttcgcggcgc agcaggagcg
     2041 gatcttgtcc gcggacgtat tgcacggcgt tcgcgcctgc gagcaggcct tgcgcggcgg
     2101 cttcttcgta gcctgtcgtg ccgttgattt gcccggcgaa aaacagtccc tcaatggttt
     2161 tggtttcgag gcttgctttg aggttgcgcg gatcgaagta gtcgtattcg atggcgtagc
     2221 cggggcgcag gatatgggcg ttttccaggc ctttcatgct gcgaacgagc gcgatttgaa
     2281 tgtcgaaggg caggctggtg gagataccgt tggggtagta ttcgtgcgtg gtcaggcctt
     2341 cgggctcgag gaaaatctgg tggctgtctt tatcggcgaa gcggttgatt ttgtcttcga
     2401 tagacggaca ataacgcggg cccacgcctt cgattttgcc ggtgaacatc gggctgcggt
     2461 cgaagcctga gcggatgatg tcgtgggttt gcgtgttggt atgcgtaatc cagcaggaca
     2521 cttggcgcgg gtgcatttcg gcgttgccgc gcacggacat gacgggaacg ggtgtgtcgc
     2581 cgggctgttc ggtcagttgg gaaaagtcaa tcgtgcgtcc gtcgatgcgc ggcggcgtgc
     2641 cggttttcag acggccttgc ggcagcttca attcgcgcaa acgtccgccc agcgatttgg
     2701 cggcggggtc gccggcgcgt ccgccctcgt agttttccaa accgatgtgg attttgccgg
     2761 acaaaaacgt gcctgcggtc aacaccacgg cgcgtgcttt aaactccacg cccatcgcgg
     2821 tgattacgcc tgaaatgcgg tcgccatcga gcgttacgtc ttcgacggct tgttggaaaa
     2881 ggtcgaggtt ttcttggttt tccaacattt cgcggatggc ggctttatat aggatacgat
     2941 ccgcctgcgc gcgcgtggcc cgtactgccg cgcctttgct ggcgttcaga cggcggaact
     3001 ggatgccgga tttgtcggtt gccaacgcca tcgcgccgcc gagcgcgtcg agttcgcgca
     3061 ccaaatgccc tttgccgatg ccgccgatgg aagggttgca cgacatttgt ccgagcgttt
     3121 caatattgtg tgtgagcaaa agcgtctgcg cgcccatacg ggcggcggcg agcgcggctt
     3181 ctgtgccggc gtgtccgccg ccgacgacga taacgtcgta ggttttgggg taaatcatgt
     3241 gggtcatgtg tgtattgcct gtcggtgttt cagacggcat ttatagtgga ttaacaaaaa
     3301 ccagtacagc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagtac
     3361 caagtgaatc ggtttcgtac tgcttgtact gtctgcggct tcgtcgcctt gtcctgattt
     3421 ttgttaaacc actatattca atatgccgtc tgaaaaacga aatggattca aaagtaaagg
     3481 gttgggattg tacgcttgtt cgccctgttt ttacagtgtg cggaaaggga aaagccgctt
     3541 cgcggggaag cggctccggt aagggcggga tttaccaaac gtcggatttg atacggcgtt
     3601 tcaggcccgg atgttcggaa agtttgaact cggggtcttt gcccattttc agcttggcgg
     3661 tgtaatcgcg cagcagcata aacgccaagg gcgagagcag caggatggcg acaaggttga
     3721 tccacgccat aatgcccatc gccatatccg ccatatccca gaccaaaggc acattggcaa
     3781 ccgcgccgaa atagacccac gccaaaacca gcatacggaa aacggcggta atcagccaat
     3841 ggcttttgat gaattggacg ttggactcgg cataggcata gttgccgata acggtggaaa
     3901 aggcaaacat aaacaggatg acggcgagga agcccgcgcc ccattgcccc acttggctga
     3961 caatcgccgc ctgcgtcaac gccgcaccgc tcaaatcgcc gtaaggctgt tggtaaatca
     4021 agatgatgaa ggcggtgcaa gaacaaacga tgatggtatc gacaaacacg cccagcattt
     4081 gaatcatacc ttgcgaaaca gggtgtttca cttcggcggc ggcggcggcg ttcggcgcgg
     4141 aacccatacc cgcctcgttg gaatacaggc cgcgtttgat gcccatcatc atcgtttgcg
     4201 aaatcagacc gccgagtaag ccgcctgctg ccgcatcgaa tttgaacgcg cccgaaaaaa
     4261 tctgaccgaa cacgtccgga atcatcggaa tattggtcaa aatgatgaaa agcgcgataa
     4321 agaggtacaa aaccgccatc aggggcacga cgatttccgc cgctttagat atgcgcctga
     4381 tgccgccgaa gataatcggc gcggttaaaa tcaccagggc gacgccgaca taatgaggct
     4441 cccaacccca tgccgctttg acggtatcgg cgatggtatt ggtctgaacc gcttcaaaca
     4501 caaagccgaa acagaaaatc aggctcaggg cgaacaacac gcccagccat ttctgcccca
     4561 gcccttgagt gatgtagtag gcagggccgc cccggaaatg gtggttgtcg tagtcgcgga
     4621 ctttaaagag ctgcgccagc gaagattcga caaacgccga actcataccg attaaggcgg
     4681 ttacccacat ccaaaacacc gcgcccggcc cgccgacttt gatggcgatt gccacgcccg
     4741 cgatattgcc cacacccacg cggctggcaa ggccggttac aaatgcctga aacggcgtga
     4801 tgccgtgagg gtcgtccccc tgtttgcggc cgccgagcat ttctttgatg ctgcgcccga
     4861 acaggcggaa ttggacaaag cccgtggtta cggtgaagaa aagccccgta cccaaaagca
     4921 tataaaccaa gtatgaccac atcggatcgt tgatggcgcc gacccagccg tgcagccatt
     4981 cggtaaagtt ctcgttcata tcgcttcctt aaagttgaaa cccgcacata ttggcggtat
     5041 gcaagcaggg tttaaatttt gtaaacgccc attctagcag attgtcaaca aaatcagaaa
     5101 aatttacatc gccgcgcggc tgcggcgtta gaatcgcatt ttgtttggag caaacacgat
     5161 gaaacagcct gtttttgccg ttacttccgg cgagcctgcc ggcatcggcc ctgatatttg
     5221 tttggacttg gcgtttgcac gcctgccctg ccgctgcgcg gtattgggcg acaaacacct
     5281 gttgcgcgcg cgcgccgaag ccttgggcaa aagcgtcgtc ttgcgcgact tcgatccgga
     5341 atcaggcggc gcggcatacg gcgagctgga agtgctgcac attccgaccg ccgaagcggt
     5401 tgaggcgggc aaactcaatc ccgccaacgc cgcctatgtg ctgcaacttt tggacaccgc
     5461 gctcgcaggc atttcagacg gcattttcga cggcatcgtt accgcgccgc tgcacaaagg
     5521 catcatcaac gacgcgcgcg caagcacagg ttttttcagc gggcacaccg aatatctggc
     5581 ggaaaaaagc ggcacggggc aggttgtgat gatgcttgcc ggcaaagacc tgcgcgtcgc
     5641 cctcgtaacg acccacctgc cgctgaaaga cgttgccgcc gccatcacgc aaccgctgat
     5701 tgaaagcgtc gcacgcattt tgcatcacga cttaaaacac aaattcggca tcaaaaatcc
     5761 caaaatccta gtcgccggac tcaaccccca cgccggcgag agcggacacc tcggacacga
     5821 agaaaccgac accattatcc ccgcattgaa aaacctgcgc cgcgaaggga taaacctcgc
     5881 cggcccgtat ccggcggata ccttgttcca accgtttatg ctcgaaggtg cggatgccgt
     5941 attggcgatg taccacgacc aagggctgcc cgtgttgaaa taccacagct tcggacaggg
     6001 cgtgaacatc acactggggc tgccctttat ccgcacctcc gtcgatcacg gcaccgcgct
     6061 cgatttggcg gcaaccggca gggcggattc cggcagcctg ataactgccg tggagaccgc
     6121 cgtcgagatg gcgcgcggca gcctttaaag atgataaaag acccgtcatt tccgcgcagg
     6181 cgggaatccg gtctgttcgg tttcagttgt ttttgggttt cgggtaattt ccaaatcgtc
     6241 attcccgcgc aggcgggaat ccagaccttc gggcgacggc aatattcaaa gattatctga
     6301 aagtttgagg ttctagattc ccgttttcac gggaatgacg aaaggttgcg ggaatccggc
     6361 ttgttcggtt tcggtttttt ttgaggtttc gggcaacttc taaaccgtca ttcccgcgca
     6421 ggcgggaatc caggcctttg ggcgacggca atattcaaag attatctgaa agtttagagg
     6481 ttctagattc ccgttttcac gggaatgacg gaatgttgtg ggaatccaga cctttgggcg
     6541 gcggcaatat tcaaagatta tctgaaagtt tgaggttcta gattcccgtt ttcacgggaa
     6601 tgacggaatg ttgcgggaat ccagaccttc gggcggcggc aatattcaaa gattatctga
     6661 aagtttagag gttctagatt cccgttttca cgggaatgac ggaatgttgt gggaatccag
     6721 acctttgggc ggcggcaata ttcaaagatt atccgaaagt ttgaggttct agattcccgt
     6781 tttcacggga atgacgaaag gttgtgggaa tctagacctt tgggcggcgg caatattcaa
     6841 aaattatctg aaagtttaga ggttctagat tcccgttttc acgggaatga cggaatgttg
     6901 cgggaatcat gggaatggag gaatgtttcg gtaatcacgg gaatggcgaa gtttcagacg
     6961 gcattgcagg tatccgaacc catgtaaaaa agaggttctg cggaacagaa cctctttttg
     7021 ccgccgtcgg ttcagccttg ccgggtttcg acttggatca tttcttcggc agggacggtt
     7081 gcgacttcag acggcttggg ctgttcggaa cggcgcaaac cgcgtccggc ttggacttcg
     7141 ggttgtgccg cccatgcctt caatgcggca gggtcggttt cgatcaggac gagtccgctg
     7201 gtttgtgcgg tttcccgtgc ctgttccgcc gcagccgtaa aggttgcggt ttcagacggc
     7261 atttcctgtg cttcggcttt cggtgtcgcg ccttcgggca ggatggcggc ggtggcacgg
     7321 cggatttttt ccgccgcatc ataaaccggt gcgtcgccgt ttgaaacggc gggagatgct
     7381 gtcggaagat ccctttctgc aaccggatcg gcaatgctga cggtaatcgg cgcgtttgcg
     7441 tcggtttcgc cgaaaacgtg cgcggcggcg gaacggactt tgtcggcggt gtcgtgaata
     7501 ttcaggtact gctcgatttt ggcggcagac ggaatattgc gttttttgcc gttttgacgg
     7561 cggtcgcgct gattgttgcg ctcgcggcgt tctttggcat ctcggctgtc gcgttcgcgg
     7621 cggttgcgtt cggatttggg cttgctgcct ttgtcttctg cggtatgcgg ttcggacggc
     7681 gtgttttccg ctgtctgaac ggttgtttcg gcaacggtgg cggtttccgg cgcggtttgg
     7741 ccgcgttcgc tgcggctgcc gttgcggcgg cgttttccgg tttgcacttc ggtttcggac
     7801 ggtgcggcat ctgcaacggt tgcggcaggc tgtatgttgc ggtcttggat ttccgcttcg
     7861 tcggcgcgtc cggcggcacg gtcgccgcgt tcgttgcggc ggcggttgcc gttgttgcgc
     7921 gtttcggctt tgtcggcacg cgcttcctgt ccggcagttt tgcctgccac ttcgcggact
     7981 tctactttgc tgccttcgcg tttgctgcgg cgcgggtttt ggcggcggtt gttggcgcgg
     8041 ctgccgctgc ggtttgccgt gctgcgtttt tcggaggttt cggcagcggg cgcggcttgg
     8101 gtttcgctgc cgccgaaaat gcgtttgagc caagctttga agctgtccca ccaggaggtt
     8161 tttttctcgg gggcggcagt cggggcgggg ctggtgtggc gcacgccttt gacggcgggt
     8221 tcgggacggg cggctttggc tttttcgccg ccgaacggtt tggcggattc gtcttcttca
     8281 ggctcggcga cgcgtttgta gctcggttcg ccgtcttctt ctacgtcgtc ggtgcggatg
     8341 cggttgattt cgtagtgcgg attttcgagg tggatgttcg gaatcaggac gacgttgaca
     8401 tccaaacgct cttccatcgc aaacagctcg gcgcgttttt cgttcagcag gaaggtggcg
     8461 acatcgacgg gcacttgtgc gcgcacttct ccggtgttgt ccttcatcgc ttcttcttga
     8521 atgatgcgta aaacgtgcag ggcggtggat tcgatgcccc gaatcacgcc ggtgccggcg
     8581 cagcgcggac aggcgacgtg gctgctttca cccaaagccg gtttcaggcg ttggcggctc
     8641 aattccaaaa gtccgaaacg ggagagtttg cccatctgca cgcgggcgcg gtcttttttg
     8701 agcgcgtcgc gtaggacgtt ttccacatca cgctggtgtt tggagttttc catgtcgatg
     8761 aagtcgatga cgaccaagcc gcccaagtcg cgcaggcgca tttgtcgggc gacttcttcg
     8821 gcggcttcca tattggtttt gaaggcggtg tcttcgatgt ccgcaccgcg tgtggcgcgt
     8881 gcggagttga catcgatgga gacgagggct tcggtgtggt cgatgacgat tgcgccgccg
     8941 gaaggcaggc tgacgctgcg agaaaacgcg ctttcgattt gatgttcgat ttggaagcgg
     9001 gaaaacagcg gcgtgtggtc ttcgtagagt ttcagacggc ctatattgcc cggcatgacg
     9061 tagctcatga actcggcaac ttggtcgtaa acttcttgat tgtccaccaa aatctcgccg
     9121 atgtcggggc ggaaatagtc gcggatggcg cggatgagca gcgagctttc cataaagagc
     9181 aggtaagggt cgtgatgcgc ttttcctgct tcttcaatcg cctgccagag ttgtttgagg
     9241 tagttcaagt cccattccaa ctcttccgcg ctgcggccga tgccggcggt acgggcgatg
     9301 atgctcatgc cgtttggaat gtcgagttcc gccatggcgg ctttcaactc ttgacgctct
     9361 tcgccttcga tacggcggga tacgccgccg ccgcgcgggt tgttcggcat caataccaga
     9421 tagcgtccgg cgaggctgat gaaggtggtc agtgccgcgc ctttgttgcc gcgctcgtct
     9481 ttttcgactt ggacgatgac ttccatacct tctttgagca cgtcctgaat acgcgccctg
     9541 ccgccttcgt agtcgcggaa atacgaacgc gatacttctt taaacggcaa gaagccgtgg
     9601 cggtcggttc cgtaatccac gaaacacgct tccagcgacg gctcgatgcg ggtaatgatg
     9661 cctttgtaga tattgccttt gcgctgttct ttgcccagcg tttcgatgtc caaatccagc
     9721 aggttttgtc cgtcgacgat ggcaacgcgt agctcttcgg cctgcgttgc gttgaataac
     9781 attcttttca tgatcacctc gtggacgggc ggcattcggg cgcgccggtc cggttcggca
     9841 ttccgtaagg ctgggttttc cgatgtcttc ggataaaacc ggtaatcagt ttttgagttg
     9901 aaaatccgca gggatgcacg ttccggagaa ccgtgtgcgg aagggtcgga tacagaaggc
     9961 tataaagatc gatgcggcgg tctgtctgcc gcgttccgaa cgctgcggtc ggaaaaatgg
    10021 gggccggctt cttcttgtta tcgtgatgcc tgtgttttgg gcggtttgcg tttgggactt
    10081 ggggcccggc tgccgtctta cttccgcgcc gaaacggcaa aatcaattca aacttgatta
    10141 cgttctgcgc ctgccggctg ggaacaggcg cagggaaaat gctttgcgga gtgcgttttt
    10201 aatataaaat tccgttttaa agtaaaccgt ttcagggggg cgcggcgggc gcgctttttg
    10261 ctgaaacgga tgttcggatt atagatgaaa acgcacgaaa taagcaaaga ttcggtcagc
    10321 ttgatagggg ttgccgaaca cgaggcgggt caacgccttg ataactatct gataaaaatc
    10381 ctcaagggtg tgcccaagag ccatatccac cgcattatcc gcgccggcga ggtgcggttg
    10441 aacaaaaaac gctgcaaacc cgacagccgt attgcggagg gggatacggt gcggattccg
    10501 cctgtgcgcg tggcggagaa ggaaatgccg tctgaaaggc gtgccgccgt gccggcgcgc
    10561 gaatttgaca ttgtttacga agacgacgcg ctgctggtga tcgataagcc ttccggcgtg
    10621 gcggtgcacg gcggcagcgg cgtgagtttc ggcgttatcg aacagttgcg ccgcgcccgt
    10681 ccggaggcgc gttatctcga actcgtccac cgtttggaca aggatacgag cggcttgttg
    10741 atggtggcga agaaacgcag cgcgctcgtc aaacttcacg aagccatccg taacgaccac
    10801 cccaaaaaaa tctaccttgc gctgggggtg ggcaaactgc cggacgacaa tttccatgtc
    10861 aaactgcccc tgttcaaata taccggcgca caaggcgaaa agatggtgcg cgtcagtgcg
    10921 gacgggcagt cggcgcatac ggtgttccgt gtgttaagcc gtttttcaga cggcattttg
    10981 cacggtgtcg ggctgtcgca cctgactttg gtgcgggcga cgttgaaaac ggggcgcacg
    11041 caccaaatcc gcgtccacct gcaatctcaa ggctgtccga ttgcgggcga cgaacgctac
    11101 ggcgattatc aggcgaaccg tcgtttgcag aagttgggtt tgaagcggat gtttttgcac
    11161 gcgtccgagc tgcacttgaa ccatccgctc acgggcgagc cgctggtgtt gaaggcggag
    11221 ctgccgccgg acttggcaca gtttgcggtg atgttggaaa acgggacgaa aatgtgaacc
    11281 ccgatgccgt ctgaagcctt cagacggcat cgggacgtga aagtatgtgg ggacagacga
    11341 atatggttga taaaaaaagc cctttgattg ccgtcagtgt cggcgaagcg tcgggcgacc
    11401 tgttgggggc gcacctgata cgcgccatcc gcaagcgttg tccgcaggcg cgttttgtcg
    11461 gtatcggcgg cgaactgatg aaggcggaag gtttcgagag cctttatgat caggagcggc
    11521 tggcggtgcg cggctttgtc gaagtggtca ggcggctgcc ggaaatttta cggatacgca
    11581 gggggctggt acgggatttg ctgtcgttga aacctgatgt ctttgtcggt atcgatgcgc
    11641 ccgattttaa tttgggtgtg gcggaaaagc tgaaacggtc ggggattccg accgtgcatt
    11701 atgtcagccc gtcggtgtgg gcgtggcggc gggaacgtgt gggcaaaatc gtgcatcagg
    11761 tcaaccgcgt gttgtgcctg ttcccgatgg agccgcagct ttatctcgat gcgggcggac
    11821 gtgcggagtt tgtcggtcat ccgatggcgc agcttatgcc cttggaagac gaccgtgaaa
    11881 cggcgcggca aactttgggc gtggatgccg gcatccccgt attcgccctg ctgcccggca
    11941 gccgcgtcag cgaaatcgac tatatggcgc cggtgttttt tcagacggca ttattgttgt
    12001 tggaacgcta tcctgccgca tgcttcctgc tgcctgccgc aacggaggcg acgaagcggc
    12061 gtttggcgga agttttgcag cggccggagt ttgccggatt gccgctgacg gtaatcgaca
    12121 gacagtctga aacggtgtgc agggcggcgg atgcggtgct ggtaacgagc ggtacggcaa
    12181 ctttggaggt ggcgctgtgc aagcgtccga tggtcatcag ctacaagatt tcgccgctga
    12241 cttatgctta tgtgaaacgc aaaatcaaag tgccgcatgt cggcctgccg aatatcctgt
    12301 tgggtaagga ggctgtgccg gagctgcttc aacatgatgc cgtaccggaa aaactggcgg
    12361 cggcgttggc ggactggtac gaacaccccg ataaggttgc cgcgctgcaa caggatttca
    12421 gggcgttgca cctgctgttg aaaaaagata cggcggattt ggccgcgcgc gcggttttgg
    12481 aagaggcggg atgttgagcg gttaatggat tatcttcttg aagcagcacg tattacaaaa
    12541 aaagggggga gaaattgtga ttaatggcac atcaaacaat aagtatttaa gaggaattcc
    12601 aaatgaagga gaactggctc gagtgggatt aaggttaaaa tataatgggc cgttaaagga
    12661 agagtttaaa caactgaaat ttcataagag tagtagaacc aacttggact caaaaaatct
    12721 taaactcatt gtttttgaaa aggtaaaata atatgacaac ttataccatt ccaaaaaaag
    12781 attatcaatt tctgtatata tatgagggca ctctattaaa ctatactttg aaaaacgatg
    12841 aattccatat catcgtccag aatgtggatt atccggactt tcctcaagag attcctacac
    12901 caaattatac agactgggta aaaattaaat tcaagcagtt cagctatctg aaatttgtct
    12961 atggatacgc tacaaagagc caagatataa atatcgataa tacattggaa cttggcgaat
    13021 taaagcagga tgatgaaatc ttggattatg gaggtgcgct ggaattgata ggcggtaggt
    13081 atgattttcc gactgctttt aggatagata tagtttgccg ggaaatagag ttagaatttt
    13141 tagatcagga gagttttaat taaacgagcc gtaacttgtc atgccgcgca agcaacttta
    13201 ttgatatgcc gatacggagc ctgttggcaa aatgccgtct gaacaatatc ttttcagacg
    13261 gcattttgta tgggggttaa cggttgttca gcccgagtac gtcctgcata tcgtacaaac
    13321 ccgttttgcc gttgacccaa actgcggcgc ggacggcacc ggcggcaaag gtcatgcggc
    13381 tgctggcctt gtgggtgatt tccacgcgct cgccgtcggt ggcgaagagg gcggtgtggt
    13441 cgccgacgat gtcgcctgcg cggacggtgg caaagccgat ggtcgacgga tcgcgcggac
    13501 cggtgtggcc ttcgcggccg taaacggcgc attgtttgag gtctctgccg agcgcgccgg
    13561 cgatgacttc gcccatgcgt aacgcggtgc cgctgggggc atcgactttg tggcggtggt
    13621 ggccttcaat gatttcgatg tcgtagcctt cgtttaatac gcgtgcgacg gtgtcgagga
    13681 tgtggaaggt gaggttgacg ccgacgctga agttggcggc gaaaacgatg cctgtttgtt
    13741 cggcggcagt gtggatagcg gctttgcccg catcgtcgaa gcctgttgtg ccgatgatga
    13801 tgttgacttg tttttcaacg catttttgca ggtgtttgag ggtgggctcg gggcgggtga
    13861 agtcgatgag tacgtcgctt tgtgcgagaa cggcgtcaac gtcgtctgaa atggcgatgc
    13921 cggttttgag tccgacggcg tagcctgcgt ccagtccgag ggcttctgag cctgagtgtt
    13981 cgagcgcgcc ggaaaggacg gtgtcgggat ggttgttgac ggcttcaacc aatacgcgtc
    14041 ccatacggcc gtttgcgccg gcgatggcga ttttgagcgg tgtcatgtgt gttccttatt
    14101 gtttgtctgc gttttgtttt acgcggaggg cttcggcggc attttgcagg gcgttgcctt
    14161 cggtgcggac gagcacgccg ttttcaaaat agacggtcag attgcttcgg tctttgatga
    14221 tgccgttgcg ggaggtgttg aaggtatagt cccagcggtc ggtatggaat gcgtcgcgca
    14281 gtatggggct gccgagcagg agcaggactt ggtctttggt cataccgggg cggagggcgg
    14341 caacggcgcg ggggtcgatt tcgttgccct gtatgacttt gagcttgtat gaggggaaca
    14401 gcgaggcgcg ttcggcactg cacgcggcaa ggccaaggag ggcggaaagg gcgaggatga
    14461 gggttttgtt cacggaaatg cctttctgtg caaatcggga tgggtagtgt aacactgctt
    14521 gaatatttta taaaagcgaa cgataatcat acgattaagc ggtatccgcc ctgtccgcgc
    14581 atcggccgcc ggtgcggttt tactattgca aactgctatg gtgcgatagt gggcaaacag
    14641 gccgaaattg cgtattataa cgtctattgt tttacagggg tattgaatat tatggaaaaa
    14701 ttcaacaata ttgcacaact gaaagacagc ggtctgaagg ttaccggccc gcgtttgaag
    14761 attttggatt tgttcgagac gcattcggaa gagcatttga gtgcggaaga tgtgtaccgc
    14821 attttgttgg aagagggtgt ggaaatcggt gtggcgacga tttaccgtgt gctgacccag
    14881 tttgagcagg cgggtattct gcaacgccat cattttgaaa cgggcaaggc ggtttatgag
    14941 ttggacaaag gcgaccacca tgaccacatc gtctgcgtga agtgcggcga ggtaacggaa
    15001 ttccacaatc ccgaaatcga agccctgcaa gacaaaatcg ctgaggaaaa cggctaccgc
    15061 atcgtcgatc acgcgcttta tatgtacggc gtgtgcagcg actgtcaggc caagggcaaa
    15121 cgttaaatcc ggacggtttg ttgttcagac ggcattcatg attttggatg ccgtctgtgt
    15181 ttttggagaa ctgccatgcg tattccgctg cttgcccctg acaattatgc ctttcccgat
    15241 cctacctatg ctttggcccg gtgcgacggg ctggtcggcg tgagcggcga tttggatgcg
    15301 gggcggctgc tcgaggcgta tcggaacggc gtgtttccgt ggttttctcg ggacggttgg
    15361 tttttttggt atgcggtcgg cccgcgtgcg gtgattgttc ccgaacgtct gcacgttccg
    15421 cgctcgctgg cgaaaacgct gcgcaacggc agctatcggg ttgcggtcaa cggctgtttt
    15481 gcggaagtgg tcgcgcattg tgcggcagcg gcgcgcccga atcaggacgg aacttggatt
    15541 gcgcccgagt ttcagacggc atatttgaag ctgcacgaaa tgggacacgc gcattcgttc
    15601 gagtgccatt atcccgatga aagcggtgaa acgaggttgg cgggcggctt ttacggcgtg
    15661 caaatcggca gggtgtttta cggcgaatcg atgttcgcat tacaaccgga tgcgtcgaaa
    15721 atcgcgtttg cctgcgccgt gccgtttttg gcggatttgg gtgtggaact gatagactgc
    15781 cagcaggaca cggaacatat gcgccgtttc ggttcggagc tgctgccgtt tgcggatttt
    15841 gccgaacgtc tgcggatgtt gaacgccgtg ccgttgaaag aggaaatcgg gcggcgcgaa
    15901 gtggcgtgca aggggctttg atggcggctt atgctccggt caggttcaaa tatggtggat
    15961 taaaattgga aatggcgaca gccgggcaaa atcactgcca aaaaatgaaa aatgccgtct
    16021 ggaacttgaa aacatcgggt ttcagacggc attttgtttg acggtttatt gcttatttga
    16081 gcgggcgcac ttcaagtccg aacatacggc gtgcggtgtt cagcatttgg cagctgaagc
    16141 cccattcgtt gtcataccat gcgaagactt taatcatgtt gccgtcaacg actttggtca
    16201 gtgttgcgtc gaagtggctg gcttcggtgg tgtggttgaa gtccatggaa accaagggca
    16261 gggtgttgta gcctaaaacg cctttgagcg cgcctgcttc cgaggcggct ttcatcagtg
    16321 cgttgatttc ttcgattgtc gtgtcgcgcg cggcttggaa gttcaaatct accaatgata
    16381 cgttgacggt cggcacgcgg atggcaagcc cgtcgagttt gtctttcaat tcgggcagta
    16441 ccagtccgac ggcttttgcc gcgccagttt tggtcggaat catgttttcc acgccgctgc
    16501 gggcgcggcg caggtctttg tggcgcacgt cggtaacggt ttggtcgttg gtcagcgcgt
    16561 ggatggtggt catcgcgcct ttgacgatgc cgacgctttc gctcaacact ttggcaaccg
    16621 gcgagaggca gttggtggtg caggaagcgt tggaaacgac ggtcatgtcg gcggtcagga
    16681 tgctgtcgtt cacgccgtac acgacggttg catcgacatc gtcgccgccc ggtgcggaaa
    16741 tgaggacttt tttcgcgccg ctttccagat gaactttggc tttttctttg ctggtgaacg
    16801 cgccggtgca ttccatgacc aaatcgacac cgagttcttt ccacggcagt tcggcagggt
    16861 tgcgggtcga gaagaagggg attttgtcgc cgttgacgat gaggttgccg ccgtcgtggg
    16921 atacgtcggc ttcaaagcgt ccgtgcacgg tgtcgaattt ggtgaggtgg gcgttggttt
    16981 caaggctgcc gctggcgttg acggcgacga tttggagttg gtcttgaatc tgataatcgt
    17041 agatggcgcg caaaacctgg cggccgatgc gtccgtagcc gttgatggcg actttgatgc
    17101 ccatggtttg ttcctttgtt gaggggttgg gtagattttc ggggcggatt atagcaaatt
    17161 tgtagtggcg tgtaattaat attttattga aaacggcgcg gccggaaggg tgggcggtaa
    17221 gatgcggacg gcacgggtgc ggcggacgga gagcttgata aaatgccgtc tgaaacggtt
    17281 ttcagacggc atatcaggga agggtcagga ggcggtattc tgtgcggctt cctgtttggc
    17341 tttgtattgt ttgagatatt cgagggcggc ggctttttcg ctgtcgctgc cgtatttcat
    17401 atcgcgttgg gcgcggcgca actcggcgcg ttcgcgggct tcggctatct gtttcgcctg
    17461 atagtctttg cggttgtcgg cggcggcgag gcggtcttgt tgggtttgcg cttttgccat
    17521 ggctttggcg atgaggtcgg cagggttaaa cgccggtttt ttcggtgtgt cgggtgtttg
    17581 cggacgcgcg ttgcggacgg cggcttcgcg ttcggcaagc atggccttgc gttcgtcggc
    17641 ttcgcgctgt ttgcgttcgt tgcgtttgag gtagcgcgca cgcgcgtgtt cggcggcggc
    17701 aaaacggctg tcggcggaca ggctgaagcg gcgcgcgcgg ggcaggacgg tgtcggcaac
    17761 gggctgcata tggatgcagt cgacggggca gggggcgacg cagagtccgc agccggtgca
    17821 ttcgtcggcg atgacggtgt gcataagttt gcccgcgccc ataatggcat cggcagggca
    17881 ggcgcggatg caggcggtgc agccgataca ggcggtttcg tctatccggg cgagtgcttt
    17941 ggcttgggtt ttggcaggtg cgacaagggg tttgccgagc agggcggaaa tgtcccgaat
    18001 gacggcttct ccgcccgggg cgcagaggtt gtacgcttcg cctgttgcga ctgcctgtgc
    18061 gtagggcagg cagccgtcgt agccgcattc gcggcattgg gtttggggaa gcaggcggtc
    18121 tatggcggcg gctgtggcgg tcatgtcggt gtgcggctca aaatcgaaag ggcgtatttt
    18181 agcagaattg tatgccgcgc ccgtttcgga tggtgcgcgg tgttttgtta taatacggcg
    18241 gcgtatgccg tttcagacgg catttttctg tattttcctg ttcggacggc ctatgaacga
    18301 attttcgctt gcccctattg tggttgtttt gctggtgtcg gtcattacgg tgatcctgtg
    18361 ccgcaagttc aacattccct ccatgctggg ctacctgctg gtgggctttt tggcggggcc
    18421 cggtatgctc agcctgattc cgaaaagcca tgcgacggat tatttgggcg aaatcgggat
    18481 tgtgttcctg atgttcagca tcggtttgga gttctcgctg cccaagttga gggcgatgag
    18541 gcggctggtg tttgggctgg gcggtttgca ggtcggcgtt acgatgctgt cggtaatggg
    18601 catactgatg ctgacgggcg tcccgttcaa ttgggcgttt gccgtgtcgg gcgcgttggc
    18661 gatgtcgtcc acggcgattg taagccggat tttgtcggaa aagacggaat tggggcagcc
    18721 gcacggtcag atggcgatgg gcgtgctgct gatgcaggac atcgccgtcg tgccgctgat
    18781 gattctgatt cccgcgctgg cgggcggagg ggacggaaat atttgggcgg ccttgggttt
    18841 ggcgtttgca aaaatgctgc tgacgctggg gctgctgttt ttcgtcggca gcaaaattat
    18901 gtcgcgatgg ttcaggatgg tggcaaaacg caaatcgtcc gaactcttta tgatcaatgt
    18961 gctgctggta accttgggtg tggcttatct gactgagctg gaaggtttgt ctatggcgtt
    19021 gggcgcattc gttgccggca tgctgctttc ggaaacggaa taccgtttcc aagtcgaaga
    19081 cgacatccgc ccgttccgcg atattttgct cggctttttc tttatcacgg tcggcatgaa
    19141 gctggacatt caggcattga tcggcggctg gcggcaggta ttgatgctgt tggcaatgct
    19201 gctggtgttg aaggcactgg ttgtgtttgc cattgccttc aaaatgaaac attcggtcgg
    19261 cgacagcctc aaaacggctt tgtatctcgc gcagggcggc gagttcggct tcgtgatgct
    19321 ggccattgct gggcagcttg atatggtttc gccagaatgg gaacaggcgg cgacggcggc
    19381 ggttctgctg tcgatgatta tcgcgccctt cctcttgggc ggcagcgatg cactggtcgg
    19441 gcgtttggtc aagtcaagct gggacatgaa gtcgctcgat ctgcacagta tgctggtaga
    19501 aaccatgagc aagtccgacc atgtgctgat tgtcggcttc ggcaggggcg ggcagacggt
    19561 cggacgcgtc cttgcccaag aggatattcc gtatttcgcg ctcgacttgg acatcgcgcg
    19621 cgtgcaggtt gccaaaaatg ccggcgagcc ggtgtcgttc ggcgatgcga aaaggcgtga
    19681 agtgttggag gcggccggtt tgggacgggc gaaaatggtg gtggttacgc tcaacaatat
    19741 gcacgaaacg caacacgttt tagacaatgt gctgtccatg tatcccaata tgcccgtata
    19801 tgtgcgcgcc accaacgacg attatgtgaa aacgtttacc gatataggtg cggaagaagc
    19861 cgtgtcggac accaaagaaa ccggactcgt gctggcaggc tatgcgatgt tgggcaacgg
    19921 cgcgtcgtat cggcacgtct atcagacgat ggcaaatatc cgccacagcc gttatgccgc
    19981 gttggaggga ctgtttgtcg gtagtgatga tgaggcagga ttcggcgaaa atggcgaaac
    20041 cgtccgccac gcctttcctt tggctgcaga agcatatgcc gtcggcaaaa cagtcggcac
    20101 gcttccgatg gcggcttacg gcatcaaact cttgttcgtc cgccgccgca ccggtcggat
    20161 tgaaaacccg gatgcctcgt ttacattgga aggcggtgac gtgttggtgg tcgcaggcaa
    20221 aaaagaagaa attatctctt ttgaaaactg gagtttgcag ggaatataaa tgaaatgccg
    20281 aaataaggct tgcgccattt ccggttattt ggtttaataa cgctttcgca aatcgca
//
LOCUS       DAWFIT010000032        20152 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFIT010000032 DAWFIT010000000
VERSION     DAWFIT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.032"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(153..353)
                     /locus_tag="WGQ85_001065"
     CDS             complement(153..353)
                     /locus_tag="WGQ85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750503.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(367..921)
                     /locus_tag="WGQ85_001066"
     CDS             complement(367..921)
                     /locus_tag="WGQ85_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750504.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1006..1506)
                     /locus_tag="WGQ85_001067"
     CDS             complement(1006..1506)
                     /locus_tag="WGQ85_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750505.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1910..3103
                     /locus_tag="WGQ85_001068"
     CDS             1910..3103
                     /locus_tag="WGQ85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0750506.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(3168..4412)
                     /gene="clpX"
                     /locus_tag="WGQ85_001069"
     CDS             complement(3168..4412)
                     /gene="clpX"
                     /locus_tag="WGQ85_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0750507.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4603..4974
                     /gene="rbfA"
                     /locus_tag="WGQ85_001070"
     CDS             4603..4974
                     /gene="rbfA"
                     /locus_tag="WGQ85_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0750508.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            4985..5509
                     /locus_tag="WGQ85_001071"
     CDS             4985..5509
                     /locus_tag="WGQ85_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750509.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            5567..6487
                     /gene="truB"
                     /locus_tag="WGQ85_001072"
     CDS             5567..6487
                     /gene="truB"
                     /locus_tag="WGQ85_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0750510.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            6807..8953
                     /locus_tag="WGQ85_001073"
                     /pseudo
     CDS             6807..8953
                     /locus_tag="WGQ85_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            8943..11723
                     /locus_tag="WGQ85_001074"
     CDS             8943..11723
                     /locus_tag="WGQ85_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0750511.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(11867..13039)
                     /locus_tag="WGQ85_001075"
     CDS             complement(11867..13039)
                     /locus_tag="WGQ85_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0750512.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            13329..13775
                     /gene="iscR"
                     /locus_tag="WGQ85_001076"
     CDS             13329..13775
                     /gene="iscR"
                     /locus_tag="WGQ85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0750513.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13804..15018
                     /locus_tag="WGQ85_001077"
     CDS             13804..15018
                     /locus_tag="WGQ85_001077"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0750514.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            15279..15665
                     /gene="iscU"
                     /locus_tag="WGQ85_001078"
     CDS             15279..15665
                     /gene="iscU"
                     /locus_tag="WGQ85_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0750515.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15752..16072
                     /gene="iscA"
                     /locus_tag="WGQ85_001079"
     CDS             15752..16072
                     /gene="iscA"
                     /locus_tag="WGQ85_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0750516.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(16032..16253)
                     /locus_tag="WGQ85_001080"
     CDS             complement(16032..16253)
                     /locus_tag="WGQ85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0750517.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            16335..16835
                     /gene="hscB"
                     /locus_tag="WGQ85_001081"
     CDS             16335..16835
                     /gene="hscB"
                     /locus_tag="WGQ85_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0750518.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16933..19683
                     /gene="gyrA"
                     /locus_tag="WGQ85_001082"
     CDS             16933..19683
                     /gene="gyrA"
                     /locus_tag="WGQ85_001082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0750519.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19840..>20152
                     /locus_tag="WGQ85_001083"
     CDS             19840..>20152
                     /locus_tag="WGQ85_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750520.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGKVEADESYFAERQNHINGIGNFWNRAKRHLRKFDGIPKAHF
                     EL"
BASE COUNT         5662 a         5059 c         4705 g         4726 t
ORIGIN      
        1 cttttccgct ttggcaacga ttgaaaattt ccgtaaattc aaatatctag attccttcct
       61 gcacgggaat gacacggaag ggtttcagat gcagggtggg cattcctgcc caccaaatcc
      121 cgcccttgca acggtgggca agaatgctcg ccctacggct tgactgttcg atatgatgcc
      181 gtctgaaaac ccaacggcgg catgacaatg ccgccctgcc aacgcacgta aatcagaatt
      241 gccatcccga catcaaacgc ttggaaacaa aatgccgtct gaaaatcaaa cggcaacata
      301 acaatgtccc taacaaatgc aaaaatgccg tctgaaagct cttcagacgg cattggtgcg
      361 ccgggtttac cgcctcctgc cgaaaccgcg catagcgggg cggcggtaat tggcgggcgg
      421 gcgcgttgtc gggcggtaac gctgcgcctg cgccgcctgt tgttttgcac ggaggctgcg
      481 cgtgttcaaa tccctgctgg tgcgcgcatt gggatgtgcg gactgatggt aggcggcacg
      541 cgcgcgccgg gcgacgggac tgtcttggtt gcctgcccgt gtgaatttgt ttgccagcgc
      601 gttgccgata aacgcgcctg ccgccgcgcc gaccaggctt tgcagcagcc agcttcctgt
      661 cgattggtcg taaatatact gctgcccgtc tttaccggta acgggttgcc cgttgttgcc
      721 gtttgcctgt gcttcggcag gaatggtgtc tttgactgct tcgggagtca gttggtaaac
      781 cgtatcgtct gcctgctgtg cgagctgctg ttgcagggct tcaatctgtt tctgctgctg
      841 ttcgagccgc gcctgcgtgt cgtcttggca ggcggcgagt gcgaatgttg cgataagcgc
      901 ggaggcgatg atttttttca tgtgtgtcct gtttgggtgg aaaatcggtt ttattgtatc
      961 gccgtcggga attttggcaa gcattctgcc gacaaatcgt gatgtttaca ggggcagggt
     1021 gtgcagtttg cggacaaatg cgaggctgtt ggcgactggg ttgcctttgt tttcgacttc
     1081 gtgttcggtt tctacggtca gcaggcggcg gtttttgtgt ttgttcaggc tcaattcggc
     1141 ttgtgcgggt gtgaaaaaca ggcggtgttt ttcggcaaag cggcgggcgc ggctgttggc
     1201 ggttttcaaa atctgaagca gcgatacgtc cagatggaag cgtcgcacgc ccaatacgag
     1261 aatccgtgcg gcaaaaaaat cggcggcatc ggtaaactcg gcggggctgc tgcggctgaa
     1321 gtcgtgccgt tcggcggcta tcagggcggc aacagtgcgg atttgcggaa tcatcgattc
     1381 gctgatgagt gtgtcgtccc gccctgcatc gagaagcatg ggggaaaaat cctgtgcatc
     1441 atcgacaata atgctacaag tgtgcagggt ttcgttttgt gcggcggttt ggggcattgc
     1501 attcatggtc attttcctga ttctgtcgtg tgttgccgaa tcgggcgacc tgtgtgaagg
     1561 taacaaaaaa gccgccccgt tttcgagcgg cctgttttgc gtatgggatg gatttcaagc
     1621 aagcgcaaaa aagtaccgca cgtctgtgtg gtaccaatag caataagcgg ttgtaaattt
     1681 tttgccttgc atgatgaaat gccgtctgaa gataaaaata ttggggagat tctaaatcaa
     1741 aacgctgccg cgcctcaagc attttatcga aatttttttg atttttcacc tatccgattg
     1801 aaaatatttc ggtttatttt taccgctgcc cgatattgtc ggcaatttcc ctttatctgc
     1861 tttgaaaaac ggtgcataat cccgagcaaa accgcaatca ggagcaatta tgcaaaacta
     1921 tctgaccccc aatttcgcct ttgccccgat gattcccgaa cgcgcttcag gcagccgcgt
     1981 ttgggatacg gaagggcgtg aatatattga tttttcaggc ggtatcgccg tcaatgcgct
     2041 gggacactgc caccctgccc ttgtcgatgc tttaaacgcg cagatgcaca agctgtggca
     2101 catttccaat atctatacga cgcgtccggc gcaggaattg gcgcaaaaat tggttgcaaa
     2161 cagttttgcc gacaaggttt ttttctgcaa ctcgggcgcg gaagcgaatg aggcggcgtt
     2221 gaagctggca aggaaatatg cgcgcgaccg tttcggcgga ggaaaaagcg aaatcgtcgc
     2281 ctgtatcaac agtttccacg gacgcacgct gtttaccgtg tccgtcggcg gccagccgaa
     2341 atacagcaag gattatgcac ccctgccgca aggcattacg cacgttccgt tcaacgatat
     2401 tgccgcgctg gaagctgccg tcggcgaaca gacctgcgcg gtcatcatcg agccgataca
     2461 gggcgaaagc ggcatcctgc ccgccactgc ggaatatttg caaaccgcgc gccgtctgtg
     2521 cgaccggcac aatgcgttgt tgattttgga cgaagttcaa accgggatgg ggcatacggg
     2581 caggctgttt gcctatgaac attacggcat tgttcccgat attttgagtt cggcaaaagc
     2641 cttgggctgc ggctttccga tcggcgcgat gctggcgaca gaaaagattg ccgccgcctt
     2701 ccaaccgggc acgcatggct cgactttcgg cggcaacccg atggcgtgcg cggtcggcag
     2761 ccgcgccttc gacatcatca acgcgccgga aaccttgcac aacgtccgca gccaggggca
     2821 gaaacttcag acggcattgc tggatttggg caggaaaaca ggcttgttct cacaggttcg
     2881 cgggatggga ctgctgctcg gctgcgtgtt ggacgcgcct tatcgcggac gcgcatccga
     2941 aataaccgcc gccgccttga aacacggcgt gatgattttg gttgcgggtg cggacgtatt
     3001 gcgtttcgcg ccttcgctgc tgttgaacga tgaggatacg gcggaaggtt tgcggcgttt
     3061 ggaacacgtg ctgacggaat ttgccgccgc aaaccgcccc taggcatcaa aaacaaaaaa
     3121 ccgcttcgga aacgtggttc aacgttccga agcggttttg tttgccatca ggactcgaac
     3181 accaattccg gttccctgcc ctcttcgatg actgccttac cgaccaccac ttttttcaag
     3241 ccttgcaaat caggcaggcg gtacatcgta tcgagcaggc agcgttcgac gatggacctc
     3301 aggccgcgcg cgccggtttt gcgctccatt gcctgacgcg cgatggaacg caatgcgcct
     3361 tcttcaaatt ccaactcgac attttccata ccgaacaagg cttgatactg cttgaccaaa
     3421 gcatttttcg gctcggtcaa aatattaatc agcgcgtcct catccagttc ttctagtgtt
     3481 gcaatcacgg gcaaacgtcc gattaattcc ggaatcagac cgaatttaat caagtcttcc
     3541 ggttcgacga tgccgaacag cttggtaatg tcggcatttt cgtccttgct gtgaacggac
     3601 gcaccgaaac cgataccgcc tttttcagta cgctggcgga tgactttttc caagcctgca
     3661 aacgcgccgc cgcagataaa caggatgttg gtggtatcga cgttgataaa ttcctgattc
     3721 ggatgcttgc ggccgccttg gggcggaacg ctggccactg taccttcaat cagtttcaac
     3781 aaggcttgtt gcacaccttc gccagatacg tcgcgggtaa tcgacgggtt gtcgcttttg
     3841 cgtgaaattt tatcgatttc gtcgatatag acaatgccgc gctgggcttt ttcgacatcg
     3901 aaatcacatt tgcccaacag tttggtaata atctgttcga catcttcgcc gacataacct
     3961 gcttcggtca gcgtggttgc atctgccatc acgaacggca catccagttt gcgcgccaaa
     4021 gattgcgcca gcagggtttt acccgatccg gtcgggccga taagcaggat gttggatttc
     4081 gacaattcga cattagctcc ggctttcggg tggcgcaggc gtttgtaatg gttgtacacc
     4141 gacaccgcca aggctttctt ggcttgttcc tgaccgataa cgtggtcgtt gaggttggcg
     4201 acgatttcgg cgggcgtggg cagcttgccg gattcttccg gctcccctcc ggcactttcc
     4261 gaaggcgtgc cgtcatttcc gtcttcatgc aatatttcaa tacagtttga gacgcattcg
     4321 tcacagataa aggcgttttc gccctcaatt aaatgtttga cgtgtgattt ggattttccg
     4381 caaaaggaac aggtacggtt ttcgttggac atggctttct ttacaatgta tgcgttacag
     4441 aaaacggcac gtgccgttcg ggttgccaag tataataact atatccgttc ttatcaatgt
     4501 attaccttaa aatcccgccg attaggctat aatacgccct ttcgcaaccg ccccggcggc
     4561 aaaaatgccg tctgaaacca aatctgaaat ctgaggatat tcatgagaaa accccaacgc
     4621 ggctacgccc gccaagaccg tgtcaaagaa caaatcatgc gcgagcttgc cgaactcgtc
     4681 cgtaccggac tgaaagaccc ccgcgccggc ttcatcaccg tcaacgaggt cgaagttacc
     4741 cgcgattaca gccacgccac cgtgttctac accgttttaa accaagatgc gcgcgaaatt
     4801 acggaagaag tgctggaaca cgcacgcgga cacctccgca gcgaattggc caaacgcatc
     4861 aagctgttca aaatccccga gctgcatttc aaatacgacg aatcgttgga acgcggtttg
     4921 aacctgtccg cccttatcga tcaagtggcg gcggaaaaac cggttgaaga ctgatactgc
     4981 taaaatgaac aatctgattc ccgaacattt agccgcctat gcacatagtg acagcctgca
     5041 aattgaaggc gtgcatcgtt gcttttcatt atcctgccaa ggtagagata ctttccacat
     5101 ccgttactat ggagagcctt ttgatggatt gatgaccgat actgataagg ctccggtaaa
     5161 aattgtggca gtagaagctg taagcggcga tgaaatcgta ttgtttgatg gcgcggaaca
     5221 tggctataac gctatgtttt gcgacaaata tagtccaaat caaaagcaaa acagaacgtt
     5281 aactgatttg gatgagtata cttaccgagt tctgatccat ctttattaca atatagacta
     5341 tgaagatgaa tatgaagatt tcgtcaattc tgaaggacaa gtccccttaa ttgatggtcg
     5401 aatcattagt tttgactcat taaaacgaaa tggctttgat gcaatcagcg ttgatctaat
     5461 tgatgaaaaa cactctgttc gtgaattatt gaatgaagaa ttaagctgac acgcaggccg
     5521 tccgaaccct ttcagacggt ctgaacatta atattcccta attaatatga ccaataaacc
     5581 cgccaaacgc ccggtcaacg gcgttcttct tctcgacaaa cccgaaggcc tttccagcaa
     5641 caccgccctg caaaaaacga ggcacttgtt ccgcgccgaa aaagccggac ataccggcgt
     5701 gctcgatcct ttggcaaccg ggcttttgcc cgtctgcttc ggcgaagcga ccaagttcgc
     5761 ccaatatctg attaatgccg acaaagccta caccgccaca ctgaaactcg gcgaagccag
     5821 cagcacgggc gatgccgaag gcgaaatcgt tgccaccgcc cgcgccgata tttccttagc
     5881 cgaatttcag acggcctgcc aagcactgac aggcaacatc cgccaagtgc cgccgatgtt
     5941 ttccgcgctc aagcacgaag gtaaaccgct gtacgaatac gcccgcaaag gcatcgtcat
     6001 cgaacgcaag gcgcgcgaca ttaccgttta cgccatcgat attgccgaat ttgacgcgcc
     6061 caaagccgtc atcgacgtac gttgcagcaa aggcacctat atccgcaccc tcagcgaaga
     6121 catcgccaaa cacatcggca cgttcgccca cctgaccgcc ctgcgccgca ccgaaaccgc
     6181 cggctttacc atcgcccaaa gccacacgct cgaagccttg gcaaacttgg acgaaacaga
     6241 acgcgacagc ctgctgctgc cctgcgacgt attggttgcc cattttcccc aaaccgtttt
     6301 aaacgattat gccgtccata tgctccgctg cgggcagcgg ccgcgtttcg aggaagacct
     6361 accttccgat acgccggtac gcgtttacac ggaaaacggc cgctttgtcg gtctagcgga
     6421 atatcaaaag gaaatatgcc gtctgaaagc cttgcgcctg atgaacacgg cggcatccgc
     6481 cgcctgaacg acggttaaaa atacgggctg tgtttgaata atgtgttgat atagtggatt
     6541 aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc
     6601 gtcgccttgt cctgattttt gttaatccac tatatttccg caaaatcacg acacactcgg
     6661 acacccgccc cgcttatcgc aactttgcga acgcccccgg aaacagcaaa gacatcaaat
     6721 aattgatttt attagaatct atttgcaaag ccatttgccg ttacacaaga atggcacatt
     6781 aaaataactg atgaggattt ataacgatga agacagacat tcaaaccgaa ttaacccaag
     6841 ccctactacc acacgaaaaa gtatgggcga acgaagaaaa aaccatttta gccaaaaaca
     6901 tcctgttgga tttggtggaa aaaaccgacc cgaccattat cggtttgtta ttgagtaatg
     6961 atgagttaaa acgccatttc tttgtggaag tgaatggtgt gctggtgttt aaattgcagg
     7021 atttccgttt tttcttggac aaacacagcg tcaataattc ctacacaaaa tacgccaacc
     7081 gcattggttt gacggacagc aaccgctttt tgaaagacag cagtgatatt gtgttggatt
     7141 ttccgtttaa agattgtgtg ttaaatggcg gacaaagcac cgaggaaggc gaagaaattt
     7201 attttaaacg caataacagc cagccagcca attatacacc aaattaaccc gaaaaagaca
     7261 agaaatcttt tttaatcaaa cgcttgcttt tgatgaaatt gaccgacttt ttgacgcaaa
     7321 agcattctca aaattctctc gctataccgc agacggcaaa caagcggttg gcgaaatcaa
     7381 acgccattca gacggcacac cggccgaaaa tcttattatc aaaggcaata atctgattgc
     7441 cctgcattcg cttgccaagc agtttaaagg caaagtgaag ctgatttata ttgatccgcc
     7501 ttattatttt gttaaaaaga aacaacaaga tagttttggt tataacacta actttaaatt
     7561 atctacttgg cttacattta tgaaaaatcg tttatcaatt gccaaagaat tattaactga
     7621 tgatgggatt attgttattt ccattgatga tgacggaaat gcttatttaa agattttgtt
     7681 agatgaaatt tttggatttg aaaattttat cggaaattta cccactatta tgaatttaaa
     7741 aggtaataat gatgaatatg cttttgcagg cactcacgaa tacacactag tctttgctaa
     7801 aaataaagat aaatctactt tttatgaatt tccaatagat gaagataatt ttttagagaa
     7861 atgggaagaa gatgaaattg ggttttataa aaaaggagct ccaatgcgag caacaggaac
     7921 cgaagataaa agagaagata gacctgaaat gttctatcct tttttggtaa aaaataacac
     7981 agttagtaca attacagatg aagaattttc tcaaatctat aataaagatt tagaagtatt
     8041 caatgatgat tttattcaaa aactaaaaga gaaatacgaa aatcttggat acaattttat
     8101 tttacctatt gctaataaac aatgggggcg ttggcgttgg gggtattcaa taaagaataa
     8161 ggcaaggctg cagacggata ttattgttag ccaatctaaa aatggaattt ccttgtataa
     8221 aaaacaaaga cctgaattag atgatttacc tacaaaaaaa cctaaaacta ttttttataa
     8281 acctgaatac agtagtggta atggaactga acagatgaaa aatctttttg gtgaaaaggc
     8341 atttaaaaat cctaaaccag aagaacttat ccaagatttt attaccatca caaccaacga
     8401 aaacgacatc gtcctagact accatttagg cagtggcaca accgccgccg ttgcccacaa
     8461 aatgaaccgc caatatatcg gtattgaaca aatggattat attgaaacgc ttgccgttga
     8521 acgcttgaaa aaagtgattg atggcgagca aggcggtatt tccaaagccg tgaattggca
     8581 aggtggtggc gagtttgttt atgccgaact tgccccattt aacgaaaccg caaaacaaca
     8641 aattttggct tgcgaagatt cagacggcat caaaacgctg tttgaaggtt tatgcgaacg
     8701 ctatttcttg aaatacaacg tcagcgtaaa tgaatttagt caaatcattc aagagcctga
     8761 atttcaatcc ttggcattag acgaacaaaa acaaatggtg cttgaaatgt tggatttaaa
     8821 tcaaatgtat gtttcattat ccgaaatgga tgatgagcaa tttgcaggtt gcttaaatga
     8881 tgacgataaa gcgttaagcc gtgaattcta tcaatcagca aaaaatcaag cggagaaaaa
     8941 agatggcgaa taataaaacg ttatttgatt gggtggaaga ccgcaaatca acgctggaag
     9001 aaatagaaca gacagatttt ttcgcgctgc ctgaatttgt agaacgcaat ttaaaatatc
     9061 cgttttttga atggcaaaaa tcggctttag aaaactttgt gatttttgac cgcgcttcaa
     9121 aattaaagga tttccctgac atcaaaaacc gccctaccca tttgctgttc aatatggcaa
     9181 caggtgctgg caaaacgatg atgatggcgg cgttgatttt gtattatttt gaaaaaggtt
     9241 atcggcattt tttgtttttc gtcaatcaaa ataatatcgt ggataaaacg gaaaataatt
     9301 ttattgaccc ggcgcacgca aaatttttat ttaccgagaa gattttgcaa ggtgatacgg
     9361 taattcctat tcgcaaagtg gaaatgttca gcccacattc agacggcatt gaaattaaat
     9421 ttaccagcat tcaaaaactg tataacgata tccacaccga gcgagaaaac caaaccacat
     9481 tggcagattt gcacaaattg aaccttgtga tgctgggcga tgaagcacac catttaaacg
     9541 cacaaaccaa aggcaaaaaa caagacgagt tggatttgga agtggaatta aaagccaatg
     9601 caggcaatgc cgaaattgaa cgcaaaggct gggagcatat ggttttggaa ttgttgctca
     9661 ataaaaatgg caatcccggc caaaatgtgc tgttggaatt taccgccacg ctgcctgaaa
     9721 atgctgacgt gcaacaaaaa tatgcggaca aaatcatcac aaaatttggc ttaaaagagt
     9781 ttttgcaaaa aggttatacc aaagaaatca atttggtatc cagtacgctg aataagaaag
     9841 agcgggtgtt gcacgcttta ttatttgctt ggtatcgcca tcaaatcgcg ttgaaatacg
     9901 gcattgccaa tttcaagcct gtgatgttgt ttagaagtaa gacgattgat gaatcaaaag
     9961 cggattattc ggcattttta aattgggcag aaaatgtaca agcggatgat tttgagtttt
    10021 tagatacgtt ttcaggcagc ctgaataata gcgacaacgc caacgaacaa ggcaaaaccc
    10081 gaaccgaaca agccctaaaa tttatgcagg aaaatggctt tgagtttgca catttggcaa
    10141 attgggtgaa acaaaattat caaaaacata atgtgattat taccaactcc gaaaccaaca
    10201 aaaacaaaac tgaaaaaacc gacagcgaaa ccgaaaaatt gctaaacaat ttggaagcgg
    10261 ctgataatcc aatccgtgcc atttttacgg tagatagatt aaccgaaggt tgggacgttc
    10321 tgaatttgtt tgatattgtg cgtttgtatg aagggcaaaa tggcggtggt tcaaataaaa
    10381 aatcaggcaa aacggctgcc gctaccgtat cggaaaagca gttgattggt cgtggcgtgc
    10441 gttattttcc atttgcgttt gaaggtaaac agccgaataa acgcaaattt gacaacgaca
    10501 tgcaacacga attgcgtatc ttggaagaat tgttttatta cacgcacgat gagcaatccc
    10561 gctatatttc agaactgaaa aatgagttgc gaaaagacgg ttatttgcct gaaaaagacg
    10621 atgataaggt gttggcaaca tttaaactca aatctgaatt tgccgataat aaggatttta
    10681 gagagttgtt aatttgggcg aataaaaaaa tccccaaccc aaacgccaaa agcaataatg
    10741 cagacagcct gcaagccaat ccgcaaacgc tttcattcca agttcacggt aatcaactgt
    10801 tgcaggaaac gcaatttaca gccgatgaaa atgatgaaac agcccgacaa atcggcacac
    10861 aaaataattt tactcaaacc ataaaaatga gtgaaatgga gcggcacatt ttcaataaag
    10921 cattacatat caaagggaaa aatagtcaat ctttattcca tttcaaccgt ttgcaaagca
    10981 agctcgacat tcaaaatcgc aatgaattgc aaaataactt gttgaaagac tggcaaattg
    11041 aatttttagg attagggcaa gacaaacagg ttcgcttgga cgataaactt gcaggctgcc
    11101 tgaaaatctt ggaaatggtt gaaaaacatc tgaatgaaag tgatatgccg tttatcggta
    11161 caaaagaatt tacacctaaa aaattatggg aaattttcgg cacgccaaaa caaaaatggg
    11221 tcaaaaaaga tgatgtaaaa acgactattg ccacgcaaaa tgattggtat gtgatgaata
    11281 attttgccgg aacgggtttg gaagaagcgt taattcaatt tatttcagcg cgtttgggcg
    11341 atttgaaatc taaatatgac gttcatttaa tccgtaatga agaagtgttt aaattgaata
    11401 actttgccga tggtgaagga tttatgccgg actttatttt attgctgaaa gataaacaaa
    11461 aatcttcttc caatggcgtg aaggactttt tgcattacca aattttcatt gagcccaaag
    11521 gtgagcattt agtggaaact gatggatgga aagaagaatt tttagaagcc atcactgtgg
    11581 aatatgggat ggataagatc ctgcaaaaag atacaccgca ttatcgtttg atcggtttac
    11641 cgttttttac tgatcatcag aaaaatggac aatttacgga gtcattccct ttaggtgcgg
    11701 catcgcttga aaaaatgaag tagtgcattg caggcaaccc cgtttgacaa aacttccttt
    11761 acaaaagggc gttttgtcag atatttaatc aacacattat taaaatacag ccaaatttta
    11821 atgccgtccg aaccctgtgt tcagacggca tcgtattttt cagtatctaa accgtttccc
    11881 tgccccaatc tttgcctctc aaaatcgaag catcgacatc ttgaatatcg cggtgtcccg
    11941 taaacgccat agatatatcc atttctttat acaggatttc cagcgcacgg gttacgcctt
    12001 cttcaccata cgcacccaaa ccatacagaa acgcccgacc tatcattgta cctttcgcac
    12061 ccaaagccca cgccttcaaa atatcctgac cgctgcggat gccgctgtcc atccaaactt
    12121 cgatgtcgct gccgactgca ctaacgatgt cgggcaaggc tttgatggcg gacacggtat
    12181 cgtcgagctg gcggccgccg tggttggaaa cgaccaatgc gtccgcaccg cttttcgctg
    12241 ctttttccgc atcttcaggt tccataatgc ctttgataat cagcttgcca ccccacaaat
    12301 ctttaatgcg tgcaacgtcg tcccagctca ggcgcgggtc gaattgttcg gaagtccatg
    12361 aagacagcga ggacaaatcg ccgacgtttt tggcgtgtcc aacgatattg cggaacgtgc
    12421 ggcgttccgt gttcagcatt ttcatacacc attcgggctt ggtcgccaga ttgattaaat
    12481 tggcgatggt cggtttcggc ggcgcggaca ggccgttttt gatgtctttg tggcgttgcc
    12541 ccaaaacctg caaatcagcg gtcaatacca atgccgaaca tttggcatcc ttcgcgcgct
    12601 taatcaggtt ttccataaac tcgcggtcgc gcatcacata aagctgaaac caaaatggtg
    12661 cggaagtgtt ctcggcaacg tcttcaatcg agcagataga catcgtggac agcgtaaacg
    12721 gaatgccgaa cttctccgcc gcccgcgccg ccaaaatttc accgtcggcg tgtgccatac
    12781 ccgtgaaacc cgtcggcgca atcgccaccg gcattttcac atcctgcccg atcattttgg
    12841 tctccaggct tcggccttcc atattgacca ataccttttg tcggaagcgg atgtctttga
    12901 aatccgaagt gttttcacgg taggtagttt ctgtccacga acccgaatcg atgtaatcgt
    12961 aaaacatacg cggcattttg cgcttggcaa cgcggcgcaa gtcttcgatg caggtcattt
    13021 tgctcaaatc acgtttcatt tgtcgccccc tgaatacctg aataacttta tatgaaatag
    13081 ataatgtata tcaatattga ttataaggca aatcatttca acatttactg cacccgccgc
    13141 cgctcccact tcaagcggca taaggtttag aattcaaaaa taatatatta aaatcaaaat
    13201 attaaaaatc aatcaatctg ccgatttaaa cagccaatcg cgcaatccgc ccccatactt
    13261 gactgaaaca ctcagatatt ggacaattcc gcccactata aaaaaagccg acacgggcaa
    13321 ccaccaccat gagactgacc accaaagggc gttttgccgt taccgctatg ctggatttgg
    13381 cgatgaacgc gcaaaccggc gccgtcaaac tcagtgccat cagcgaacgc caaaacatat
    13441 ccctctccta tctcgagcaa ttgttcggca aactccgccg cgccggactt gttgaaagcc
    13501 tgcgcgggcc cggcggcggc tacatcctcg ccgcaccggc ggcacgcatc aacatcgccc
    13561 aaatcatcgc cgccgccgaa gaccggctgg acgcaaccca atgcggcagc aaagccaact
    13621 gccaccacgg cgcgccctgc ctgacgcacg acctttggga gaatttaaac aaaaccatca
    13681 acgactacct cggcagcgtt accctgcaaa gcatcatcga acagaaaaac aacggcgacg
    13741 gcagccgcgt cgtccaattt acacacatcc attaaataac acccgaaaaa gaaagagcaa
    13801 accatgaccg tcaaaacccc cgtttacctc gactacgccg ccaccaccac cgttgacaaa
    13861 cgcgttgccg aaaaaatgat tccctatctg accgaaacct tcggcaaccc cgcctccaac
    13921 agccacgcat tcggctggga agcagaagag gccgtcgaaa aagcccgcgc cgacatcgct
    13981 gccctgatta atgccgaccc caaagaaatc gtcttcacca gcggcgcgac cgagtccgac
    14041 aacctcgcca tcaaaggcgc ggcaaacttc tacaaaacca aaggcaaaca cctcattacc
    14101 gtcaaaaccg aacataaagc cgttttagat acaatgcgcg aactcgaacg ccaaggtttt
    14161 gaagtaacct acctcggcgt tcaagaaaac ggtttgattg atttggaaga actcaaagcc
    14221 gccatccgcg acgacaccat cctcgtttcc gtaatgtggg caaacaacga aatcggcgtg
    14281 gtgcaagaca ttcccgccat cggcgaaatc tgccgcgaac gcaaaatcgt cttccacgtc
    14341 gatgccgcac aagcctgcgg caaagtgcct gtcgatgtcg aagccgccaa aatcgacctg
    14401 ctctcgatgt ccgcgcacaa agtgtacggc cccaaaggca tcggcgcgct gtacgtccgc
    14461 cgcaaacccc gcgtccgcct cgaagcccaa atgcacggcg gcggccacga acgcggcttc
    14521 cgcagcggca cattgccgac ccaccaaatc gtcggtatgg gcgaagcctt ccgcatcgcc
    14581 aaagaagaac tcgaacaaga tatggcgcac taccgcaaac tgcgcgatat tttcctcaaa
    14641 ggtatcgaag gcatcgaaga agtctatatc aacggcgacc tcgaacatcg cgtcccgaac
    14701 aacctaaacg tcagcttcaa cttcgtcgaa ggcgaaagcc tgattatggc agtgaaagaa
    14761 ctcgccgtat ccagcggctc cgcctgcacc tccgcctcgc tcgaacccag ctacgtcctg
    14821 cgcgcattgg gcagaaatga cgaattggcg cactcgtccc tgcgcatcac cttcggtcgc
    14881 atgaccactg aagaagaagt gcaatttgcc gccgaactga ttaaatccaa aatcggcaaa
    14941 ctgcgcgaac tgtcgccgtt gtgggaaatg ttcaaagacg ggattgattt gaactcgatt
    15001 gaatgggcgg cgcattaaag cgtaccaaca tgccatccga accttcagac ggcattccaa
    15061 aaacaaagca atcaagagaa aaatatgaac gaacaagatt tagatttgga caacctcgac
    15121 aacctgcttg acgatttcga cggcgttacc gtggaaggcg gcgtcgattc ggaaaacgac
    15181 gacggctgtg aaggcggagc gtgcaaaatc taactcaggc cgtctgaaaa ccggcaaaca
    15241 aaccacttaa atcatcaaaa cattaaggaa accacatcat ggcatacagc gataaagtaa
    15301 tcgaccacta cgaaaatccc cgcaacgtcg gtactttcga caaaaacgac gaatctgtcg
    15361 gcaccggcat ggtcggcgca ccggcctgcg gcgacgtgat gcgcctgcaa atcaaagtga
    15421 acgatgaagg catcatcgaa gatgcgaaat tcaaaactta cggctgcggt tccgccatcg
    15481 cttcgtccag cctgattacc gagtgggtta aaggcaaaag cctggatgac gcgctggcaa
    15541 tcaaaaacag cgaaatcgcc gaggagttgg aattgccgcc ggtaaaaatc cactgctcca
    15601 tcttggctga agatgcggta aaagcggccg ttgccgacta ccgcaaacgt caggaaaaca
    15661 gataaagccc ttcagacggc atcgtcccgc aatgccgtcc gaaccacccg ccgcttcagg
    15721 tccgtccggg gcggtacaac aaggaagaaa tatgattacc cttaccgaga atgccgcaaa
    15781 acacatcaat gactatctcg ccaaacgcgg caaaggcttg ggcgtacgct tgggtgtgaa
    15841 aaccagcggc tgctcgggga tggcgtacaa ccttgaattt gtcgacgaag ccgatggcga
    15901 cgacctgatt ttcgaaggac acggcgcgcg catttatatc gatccgaaaa gcctggttta
    15961 tctggatggc acgcaagtcg attacaccaa agaaggtttg caggaaggtt tcaaatttga
    16021 aaaccccaat gtcaaagact cctgcggctg cggcgaaagc ttccatgttt aagacataaa
    16081 aatggcggga ccgtatcaaa accgtcccgc cattttttcg cttcctgcct gttgtagctg
    16141 cctttgcctt ttcttttccg ttccaccttg tgtcggaaca aatcagattt cactaaggct
    16201 tttaaagcat tgtcgcgtat tttgccttta ttgtgctgca ctttgccgcc catattcagt
    16261 cctttcgttt aagaagcggc agattataag gcaaaaacag ttttctgcca aaatcttaca
    16321 tttatcatcc tactatgtcc caatatttca ccctcttccg gattgaaccc gctttcgata
    16381 tcgacaccga aaacttggaa caaacctacc gcgccttggc cgcccgtttc catcccgata
    16441 aattcgcttc agcttccgcc tttgagcaaa agcaggcagt gatgatgtct tccaccatca
    16501 acgatgccta ccgcaccttg aaaaacccca tcgaccgcgc cgcctacctg ctgaaaacat
    16561 cgggcatcga tgccgacgcg ccggagcata ccgctttcgc ccccgaattc cttatgcagc
    16621 aaatggaatg gcgcgaaacg ctgatggagg cacgggcagg caacgacctt gaatccttga
    16681 aaaatctcga caacgaaatc cgcgacgaac aagaaaaact gttctgcggt ctgaaacagt
    16741 cgtttgcccg acaagattac gacacagccg cacaacaagt ccgccaaggc aggtttctcg
    16801 acaaactccg caacgaaatt tcctcggcat tataatccgc accgtgtttc agacggcgta
    16861 accgccgcac cgttccgcgt caaaatatgc taaaataagc aacaattttt tgccatacga
    16921 aacattgaaa ccatgaccga cgcaaccatc cgccacgacc acaaattcgc cctcgaaacc
    16981 ctgccggtaa gccttgaaga cgaaatgcgc aaaagctatc tcgactacgc catgagcgtc
    17041 attgtcgggc gcgcgctgcc ggacgttcgc gacggtctca agccggtaca ccgccgcgta
    17101 ctgtacgcga tgcacgagct gaaaaacaac tggaatgccg cctacaaaaa atcggcgcgc
    17161 attgtcggcg acgtcatcgg taaataccac ccccacggcg ataccgccgt atacgacacc
    17221 atcgtccgta tggcgcaaaa tttcgctatg cgttatgtgc tgatagacgg acagggcaac
    17281 ttcggatcgg tggacgggct tgccgccgca gccatgcgct acaccgaaat ccgcatggcg
    17341 aaaatttccc acgaaatgct ggcagacatt gaggaagaaa ccgtcaattt cggcccgaac
    17401 tacgacggta gcgaacacga gccgcttgta ctgccgaccc gtttccccac actgctcgtc
    17461 aacggctcgt ccggcatcgc cgtcggcatg gcgaccaata tcccgccgca caacctttct
    17521 gataccgtca atgcctgcct gcgcctgctc gatgcacccg acaccgaaat cgacgaactg
    17581 atcgacatta tccaagcccc cgacttcccg accggggcaa ccatctacgg cttgagcggc
    17641 gtgcgcgaag gctataaaac aggccgcggc cgcgtcgtta tgcgcggtaa gacccatatc
    17701 gaacccatag gcagaaacgg cgaacgcgaa gccatcgtta tcgacgaaat cccctatcag
    17761 gtcaacaaag ccaagctggt cgagaaaatc ggcgatttgg ttcgagaaaa aacactggaa
    17821 ggcatttccg agctccgcga cgaatccgac aaatccggta tgcgcgtcgt tatcgagctg
    17881 aaacgcaacg aaaatgccga agtcgtctta aaccaactct acaaactgac tccgctgcaa
    17941 gacagtttcg gcatcaatat ggtggttttg gtcgacggac aaccgcgcct gttgaacctg
    18001 aaacagattc tctccgaatt cctgcgccac cgccgcgaag tcgttacccg acgtacgctt
    18061 ttccggctga agaaggcacg ccatgaaggg catatcgccg aaggcaaagc ggtcgcactg
    18121 tccaatatcg atgaaatcat caagctcatc aaagaatcgc ccaacgcagc cgaggccaaa
    18181 gacaaactgc ttgcgcgtcc ttggcgcagc agcctcgttg aagaaatgct gacgcgttcc
    18241 ggtctggatt tggaaatgat gcgtccggaa ggattggctg caaacatcgg cttgaaagag
    18301 caaggttatt acctgagcga gattcaggca gatgctattt tacgcatgag cctgcgaaac
    18361 ctgaccggcc tcgatcaaga agaaattgtc gaaagctaca aaaacctgat gggtaaaatc
    18421 atcgactttg tggatatcct ctccaaaccc gaacgcatta cccaaatcat ccgcgacgaa
    18481 ctggaagaaa tcaaaaccaa ctatggcgac gaacgccgca gcgaaatcaa cccgttcggt
    18541 ggcgacattg ccgatgaaga cctgattccg caacgcgaaa tggtcgttac cctgacacat
    18601 ggcggctata tcaaaaccca gccgaccacc gactatcagg cgcagcgtcg cggcgggcgc
    18661 ggcaaacagg cggctgccac caaagacgaa gactttatcg aaaccctgtt tgttgccaac
    18721 acgcatgatt atttgatgtg ctttaccaat ttgggcaagt gtcattggat taaggtttac
    18781 aaactgcccg aaggcggacg caacagccgc ggccgtccga ttaacaacgt catccagttg
    18841 gaagaaggcg aaaaagtcag cgcgattctg gcagtacgcg agttccccga agaccaatac
    18901 gtcttcttcg ccaccgcgca gggaatggtg aaaaaagtcc aactttccgc ctttaaaaac
    18961 gtccgcgccc aaggcattaa agccatcgcg ctcaaagaag gcgactacct cgtcggcgct
    19021 gcgcaaacag gcggtgcgga cgacatcatg ctgttctcca acttaggtaa agccatccgc
    19081 ttcaacgaat actgggaaaa atccggcaac gacgaagcgg aagatgccga catcgaaacc
    19141 gaaatttcag acggcatcga agatgaaacc gccgacagcg aaaacgcact gccgagcggc
    19201 aaacacggtg ttcgcccgtc cggtcgcggc agcggcggtt tgcgcggtat gcgcctgcct
    19261 gccgacggca aaatcgtcag cctgattacc ttcgcccctg aaaccgaaga aagcggtttg
    19321 caagttttaa ccgccaccgc caacggatac ggaaaacgca ccccgattgc cgattacagc
    19381 cgcaaaaaca aaggcgggca aggcaatatt gccattaaca ccggcgagcg aaacggcgat
    19441 ttggtcgccg caaccttggt cggcgaaacc gacgatttga tgctgattac cagcggcggc
    19501 gtacttatcc gcaccaaagt cgaacaaatc cgcgaaaccg gccgcgccgc agcaggcgtg
    19561 aaactgatta acttggacga aggcgaaacc ttggtatcgc tggaacgtgt tgccgaagac
    19621 gaatccgaac tctccgacgc ttctgtaatt tccaatgtaa ccgaaccgga agtcgagaac
    19681 tgaaaatcat ctcccgatgc cgtctgaaga ttcagacggc atttatttta tccctcatcc
    19741 gtcatccagc ttctcacaat atagcggatt atagtcaatt aaaaacaagg ggctgtccta
    19801 gataactagg gaaattcaaa ttaagttaga gttgccccta tgagaaaaag tcgtctaagc
    19861 cggtataaac aaaataaact cattgaactg tttgtcgcag gtgtaactgc aagaacggca
    19921 gcagagttag taggcgttaa taaaaatacc gcagcctatt attttcatcg tttacgatta
    19981 cttatttatc aaaacagtcc gcatttggaa atgtttgacg gcaaagtaga agcagatgaa
    20041 agttattttg ctgaacgaca aaaccatatc aatggaattg ggaacttttg gaaccgggca
    20101 aaacgtcatt tacgcaagtt tgacggcatt cccaaagcgc attttgagct gt
//
LOCUS       DAWFIT010000033        19715 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFIT010000033 DAWFIT010000000
VERSION     DAWFIT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.033"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGQ85_001084"
     CDS             complement(60..1118)
                     /gene="dinB"
                     /locus_tag="WGQ85_001084"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0750521.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYHQVSAQIHTVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1171..1272)
                     /locus_tag="WGQ85_001085"
                     /pseudo
     CDS             complement(1171..1272)
                     /locus_tag="WGQ85_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1366..4456)
                     /locus_tag="WGQ85_001086"
                     /pseudo
     CDS             complement(1366..4456)
                     /locus_tag="WGQ85_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            4730..5509
                     /locus_tag="WGQ85_001087"
     CDS             4730..5509
                     /locus_tag="WGQ85_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0750522.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(5718..7130)
                     /locus_tag="WGQ85_001088"
     CDS             complement(5718..7130)
                     /locus_tag="WGQ85_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0750523.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDVFKAKFKTDRKNIKEIG"
     gene            7221..7297
                     /locus_tag="WGQ85_001089"
     tRNA            7221..7297
                     /locus_tag="WGQ85_001089"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7255..7257,aa:Arg,seq:acg)
     gene            7315..7389
                     /locus_tag="WGQ85_001090"
     tRNA            7315..7389
                     /locus_tag="WGQ85_001090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7348..7350,aa:Glu,seq:ttc)
     gene            7945..9120
                     /locus_tag="WGQ85_001091"
     CDS             7945..9120
                     /locus_tag="WGQ85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0750524.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9330..9878)
                     /locus_tag="WGQ85_001092"
     CDS             complement(9330..9878)
                     /locus_tag="WGQ85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0750525.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(9881..11377)
                     /gene="ccoG"
                     /locus_tag="WGQ85_001093"
     CDS             complement(9881..11377)
                     /gene="ccoG"
                     /locus_tag="WGQ85_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0750526.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            11905..13884
                     /gene="tkt"
                     /locus_tag="WGQ85_001094"
     CDS             11905..13884
                     /gene="tkt"
                     /locus_tag="WGQ85_001094"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0750527.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYDWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVTWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            14025..15413
                     /gene="fumC"
                     /locus_tag="WGQ85_001095"
     CDS             14025..15413
                     /gene="fumC"
                     /locus_tag="WGQ85_001095"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0750528.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(15478..16401)
                     /locus_tag="WGQ85_001096"
     CDS             complement(15478..16401)
                     /locus_tag="WGQ85_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0750529.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAVGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            16573..16713
                     /locus_tag="WGQ85_001097"
     CDS             16573..16713
                     /locus_tag="WGQ85_001097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750530.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(16773..17297)
                     /locus_tag="WGQ85_001098"
     CDS             complement(16773..17297)
                     /locus_tag="WGQ85_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0750531.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(17301..18683)
                     /locus_tag="WGQ85_001099"
     CDS             complement(17301..18683)
                     /locus_tag="WGQ85_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0750532.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(18794..19417)
                     /locus_tag="WGQ85_001100"
     CDS             complement(18794..19417)
                     /locus_tag="WGQ85_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0750533.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         4840 a         5684 c         4719 g         4472 t
ORIGIN      
        1 ccatgccgtc tgaaaaccgt tcagacggca tattttttgg acggcgcgcg taaagcggtt
       61 tacgcccaca aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata
      121 aggcggaacg cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc
      181 agcagagctg cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg
      241 aaatcgtagg tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt
      301 tgccgccaca agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg
      361 tcttcgggca gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg
      421 cgttcgtccg taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt
      481 aagagttcgc cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc
      541 atttttttca gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa
      601 aatgccatga ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac
      661 gcgattttcg ccagaaattt gttcggcgcg atgcctgcgg atgcggtcaa acctgtttcc
      721 gcaaaaatgg cggcacggat ttctttggca acttcgctgg cgtaagggat gtttttgaaa
      781 ttacgggtaa cgtcaagata ggcttcgtcc agcgacaggg gttcgattaa atcggtataa
      841 cgtctgaata cagtatgaat ctgcgcggaa acctgatggt acaagtcgaa atgcggcggc
      901 acatacaccg cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc
      961 ccgaactgcc gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg
     1021 acaaccaccg gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcatagaac
     1081 gcgtccatgt cgatgtggat gattttgcgt gaagacatcg gttctgctga ggataaaagg
     1141 gatattctac tgccggcatc gggcaaattc caaatatagt ggattaaatt taaaccagta
     1201 cggcgttgcc tcgccttgtc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     1261 atttttgtta atccactata tacgccctga taggcctgcc tccataaaaa tgccgtctga
     1321 aacataccct gtttcagacg gcatccgcaa aactacggtt tccaattaaa actgccaatc
     1381 cagtttcatg ctgacagtgc gcggctctcc atagaagttg tttgcgccgc gcgtacggtt
     1441 gtagttgttc tcaaaataag tgcgtccgtt taagttcgta ccgatgaggc tcaatttggc
     1501 gtgtttgccc aattcgtaac ggacgaaacc gtctatcagc ccgtagccgc cctgcctgat
     1561 gttatacaga ctgcttgtgc cgctttgtgc ggacacgccg ccgccgacgg tcagccccgt
     1621 attcggtata tggaagctcg ttccgaaacg gaatatgtgc acgggtgtga aattgctgaa
     1681 gttgtacggg tctgcgcctg tgtttttggc gaggcgttcg gcgttgactt cggcggcgtt
     1741 tttgtagcgg ctcttgttgt aggtgtaacc cgcaaagact ttccaatctt cgttcaactc
     1801 acccgacaac tcgaattccg cacccctgct gaccactttg cctatcggtt tggcaacggt
     1861 ttggaacgac ccctgcttgc cgcctgctcc gggaacatag ccaaaatcga cgaccgtgcg
     1921 gtttttctgt tcgaggtaaa acaatgcgaa cgaagcattc agccgtcctt gcaagaacgc
     1981 gcctttccag cctacctcat agtttgtgcc gaccaaaggc ggtaaaacgg ttttggcact
     2041 gacatcgaca ttatcctgct gtttgaagat tttggtataa cttccgtaaa tactctgttg
     2101 cggtgtcaaa tcataggtaa tgcctgcata gggcgtcaat ttatgacctt gcatcttggc
     2161 cgtataatgg tcctgatccg ccttaatgct cgatgccgtc tgaaaatcgc ttgccggctg
     2221 cccatagcgg acaggcatat ctttggtttg cgaggtttca tagcgcgtgt agtgcagccc
     2281 gcccaaaaga tgcagtcggc cggttacgtt gaaacgcgtg ccggcagtca gcgaatgggt
     2341 tttgttggtg ttgaggtatt tggcgtagtt atacagcgca ggaacatgat cgtcggccac
     2401 tttgacggtt ttccaaaccg gcaccgtacc ggaaaaaccg gtaaaggcag gcgagccgtc
     2461 gggattggtc tcctgaatct tgttgccttt ttcgtccagc tcatatacat cgacatatac
     2521 cggtgtccgg ctgccgctgt attcgtcata gtaatatacc tgtttgcctt cggcatcgag
     2581 cttgggctcg gtttttattt tcttggcgtt cctgcattct tcggcataaa cggtacggtt
     2641 gcctttttca tcgtacgcct tccaatcggg ttctttatgc cccctgacca aaggagacga
     2701 caaatcgccg tccggctcct cctgacaact tcccgcatac acgccgtgcg ttgcccccgt
     2761 attcggacgt actctgtggc ggcgttcgta gatttccaga tattcggaac gtatcttttc
     2821 atcgccgtag gcatagccga caaagaaatc gtgttcccgc ccgaacagcc catatgtgcc
     2881 ggtcaggtca agtttgattc cccattggcg gtcgtctttg gtatgccgca acggcatata
     2941 actgtatcgt cggttggcgg tagccttacg attaaaggaa gagtcataca ggctgtttgg
     3001 aaaacgttgc gccgcattat taaagatgcc ctccttcgca agggctttat cgacaaattc
     3061 cgccttgtcg gcatcaacgc ccggatcccc cccaagaacc ttgacagata aagtccagcg
     3121 caaaaggggc actcatacac tcgtcaaaac cggctttgcg ttctgcggca cggcggctgc
     3181 gatactgttc gaaagcagta ttatcgaaac ggtttttaac aaaatcgtct ttgcgctccc
     3241 ggtattcctt ggcggtttca tcacgatatg ctttcagttt ctccaatgcc ttatctttcg
     3301 gctcgaacgg gatgacttcg tttttttcag tcaaaaagcc taccgcatcc tcacccgaca
     3361 aacccgccgc atgttcgttt ttcagaaaaa actgccccac cttcgcatcg gattcattct
     3421 tggtataaga cacttcggca ttgagctgcc aaccgttgtc aaacacatgt ttgaaacctg
     3481 agaaaaggtt gtatttgtca gcactcaacc gcgaccaatc ctcccccaaa taagtgttgc
     3541 gcggcagttg caaaggccgg ttgcaggcag gcgttgaact gaacggggca gttttctgat
     3601 tttcacagga caaaataatg cccgaaaaat caggaacctc cctactcttc tgatacatgc
     3661 cgcccaaagt aagcacactg ctgtcgcccg catcggcttc ggcaatgccg taaaccatat
     3721 gtttcctgcc ccaaacgcgg tctttaaacg attttttata ctcttccgca cccaccaacc
     3781 ttccgcgtaa ggtattcgcc ttattcaggc tgcctgaaac atccaacact gcacgccggc
     3841 tgccgcgatg gtcggcggtc agctctccgg tatgtttgaa agaagcggta ggtcgcttac
     3901 ggatcaaatt gacggttcca cccggctctg aattggattg ggtcaacccc gttgcacccc
     3961 gtacaacttc aatatggtca taaaccgcca aatcggtact cggagacacg tcgattttcg
     4021 ccgtatatcc cgaacggcct gcaacattga cggtaatacc gtcttcacca atctgatcaa
     4081 tatagaaacc gcgtgacaaa aaccgcgtct gcaagcctga atcgcgcaca acgttgacac
     4141 ccgtcgtgtt tttcattgcc tcttcaagcg tatgcaccgc cttatcgtca aggcggctgc
     4201 gcgtgatgac gctgaccgac tgcggcgtat ccttgcccgc aatcctcata cctgtggcgg
     4261 tggacatccg atctatcgta taagaacggg tcttctcggt cttgcccgac agagcatgag
     4321 agccgcgtac atcgactgta tccaaactga cggtattgcc gtctgaaaca ggcacaacac
     4381 cgtctgcaaa agaaccaccg taagccgata acagcataac ggtcagaatt ttaagtgaaa
     4441 aatgattttg attcatagag acctctgtaa tatgcaagtg tgcaaatcgt ccaaaggccc
     4501 tcacaactgt tttgattttt tatattaatt gaaaaaaagt aattctcaat taaatttata
     4561 gatgggattg tattcccatt ttgacaaaaa acaaactgct ccttaccgtt tatttcaaaa
     4621 aacgataata ttgtattgaa aaatatccga atttaaatat agaccgtcaa tgcggaaaaa
     4681 aacacccaaa ttggctataa tcccgacaaa cacactcaag gacaacaaca tggcagcctc
     4741 acccgaagca aaattcactg aagaaaagat tttgtgggtc aaacaccaca cgccgaaact
     4801 catcactttc gccatcagcc gtcccgaatc ctaccgtttt aaagcgggac agttctcccg
     4861 actcggtttc tacgaagggg aaggctttat ttggcgtgcc tactccatcg tttccgcaga
     4921 atatgccgac acgctcgaat attttgccgt actcatccaa gacggcccca tgtcggcccg
     4981 tttcgccaaa atgcaacagg gcgacaccat cctgctcgat aaaaatgcca ccggcttcct
     5041 cctgcccgaa cgcttccccg acggcaagga tttggtgatg ctctgcaccg gctccggcat
     5101 cgcccccttc ctttccattc tcgaacaacc cgaaatccgg cagcgtttcg ataccgtcaa
     5161 cctgatacat tccgtatctt ttcccgaaga attgattttc aacgaccggc tcgccgcatt
     5221 gtccgaacac cccctggtag gcgaatacgg acactcgttc cgtttcgtcc ctgttaccac
     5281 ccgtgccgcc aacccctcgg gcttaagcgg aaaacgcatt ccggaactct taaaaaacag
     5341 cagcatcgaa caggcgctgc ataccaagtt caccccggaa tccacacggt ttatgatttg
     5401 cggcaacccg gaaatggtca aagacacttt ccaaacgcta ctcgacatgg gttacgccat
     5461 gcaccgcaac cgcattcccg gtcaaatcat gatggaaaac ggcttctaaa aaccaccctg
     5521 cttgtccgat gccttcggat ggaagggcaa accgacacgg cacgaaaacc gcgtcggcaa
     5581 aaatgccgcc tgaaaccttc agacggcatt ttttcggata catacaaacg gcaacacatt
     5641 ccaacccaaa ccgattaggc aatcaacacg gtgacggctg tttacatact tgccggcttt
     5701 caccaaccga tatcgattta accgatttcc ttaatatttt tcctgtccgt tttaaacttc
     5761 gccttaaaca catccggcaa atctttatcg aaataccaaa gcccgtcacc catttccaat
     5821 gcgccgccca ttccatgcag aacaactttt tcccccgtta aaggatcagt ttcggtaatt
     5881 tctacctcat gcatttttcc ccaatccaaa ataggcaact tgtgtgcaaa cgccaaaacc
     5941 tgttcgtcag aacggccgca cgccttatcg catcggcctg aaacatatac gggacaaggc
     6001 tcatcctctg cataaccgtc cggaaggata ccggctgagg caggacacaa tccgtatgtt
     6061 tccgcccacg acgacaaagc ccgtctcgcc gcctttttat tggcaaataa tccggtaggc
     6121 ggattatccg tcacaccgtt tttcaaagcc gctgttttcg cattcaacat gccgtctgaa
     6181 cctttttcaa acctgacggt cgtaaatgtt ttaagcagat ttttggcaga tacataacaa
     6241 cccgaatgat aacgtccgac caattccgct ttaatcttat atgcatgcag gctgcccaat
     6301 gcaggaaaaa aacggacttc ctccgtattg caccaatcaa acggggcttt tccggaatcc
     6361 aataaagccg aaacctcgct atatacccgt tcaaacgtac cgatgtaatt tactttccct
     6421 ccgccgtcga aacaagccag cacccccata ccgtcaggca aaccgtacaa ctgttccctc
     6481 aaccgttcgg gcagcgcggc aggcagcggt ttcggattca tcaaacggaa acactgcctg
     6541 atccatgcct caaccccatg ttccgacaaa ctgtattcca aataatcaca caatgccgac
     6601 acatccgcca tcgcacgatg cctgtcttct acaacaatcc ccaacctttc gatgatactg
     6661 tccaggctgt gcttgtaaaa ttgcggatac agacaccggg acagttgcac actgcacaaa
     6721 gcaggcgatg aaaacccgat gcccgcacga tgaaactcat gctttaaaaa cgtatagtcg
     6781 aaacggctgt tatgtgcaac cagcacacaa cccttcaata ccgaaaacaa ctcgccggca
     6841 atctctgcaa aaacaggcgc atcggcaacc atgccgtctg aaatccccgt cagccccgcc
     6901 acaaactgcg gaatcggttt ttgaggatta accaaccact catgcctcac cacccttccc
     6961 tgctcaaact tgaccaaagc cacttcggtt accctgtctt catacagatt gccgcccgtc
     7021 gattccaaat cgaccacggc aacaggcatt ccaaaccgta aaaatacctt ttccagcaag
     7081 ggccagcggg aagcaacaat cattttattc tctttaaatt caaacaacaa accgatattt
     7141 tacactttta aggcatttca tccaacaaaa caattgacag aatccgatga ttaccctaaa
     7201 attcaaatct ttcttgcagt gcacccgtag ctcagttgga tagagtatct ggctacgaac
     7261 cagagggtcg ggcgttcgaa tcgctccggg tgcgccagta agaaaataca atatgcgccc
     7321 atcgtctagc ggttaggaca tcgccctttc acggcggtaa ccggggttcg attccccgtg
     7381 ggcgtgccaa attcccaatc cctgagatta tccctcgggg atttcttatt acctcgacaa
     7441 ctcgttacca tatctttacc ccccttcatc agactctcag acgtaatcga atcatattca
     7501 aacctttgcc atgcaaaccg atatcacgta accggatgcg gtgtccgtcc aacattttac
     7561 ccgattgaaa cgcctgatat attgcacccc atcaacgtgg cattactttt cttaacaatc
     7621 ccctttgaca gcaactgact agggcttttt tatgccatca tcaaatttat agtggattaa
     7681 ctttaaacca gtacggcgtt gcctcgcctt tccgtactat ttgtactgtc tgcggcttcg
     7741 tcgccttgtc ctgatttttg ttaattcact ataatatttt ctctcccgat tgaaacaggc
     7801 gtaacagaat atgcccgaag ctccggctgc tttcttgttt accgccgcga tatttagagt
     7861 ataataccaa atttgagcaa tagttctaaa acagttagaa ccatttttca tgagcctgac
     7921 tgattcgtac actcggagaa actgatgcag aatatttttg accctttggt tattcgtgga
     7981 aaatccctta cccccatcgt gcaaggcggt atgggggtcg gtgtttccgc atcgggttta
     8041 tccagcgcgg tggcacgtga aaacggtatc ggaacgattg ccagtgtgga tttgcgccac
     8101 cttcacgaag acctactcgc cgaatcacaa atcaatccga gtgaagagaa atatacatct
     8161 ttgaactgta ccgcattaga cagggaaatc caaaaagcca aaagcgcttc agagggaaaa
     8221 ggactgattg cggtcaacgt gatgaaggcg gtcaaagacc acgccgcgta tgtccgccag
     8281 gcttgcgaat caggggcgga tgcggttgta atgggtgccg gtctgccttt agacctgccg
     8341 gaaatgaccg agggctatca taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc
     8401 ggtattaata tcgtcttgaa acgttggatg aaaaaaggca tattgcccga tgcgattgta
     8461 gtcgaacatc ctgcccacgc ggccggacat ttgggtgcat caaccgttga aggcgtaaac
     8521 gatgccaagt tcgacttcaa acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta
     8581 gggctggaaa gcgaaaaaat cccgcttatt cttgcgggag gcatggcaaa ttttgaaaaa
     8641 gtcaaaaccg ccctaaagaa ctggggagca tccgccgttc aaatcggtac ggcttttgcc
     8701 gttaccgaag aaggagatgc acaccttaac ttcaaaaaaa cgctcgccgg tgcggaaact
     8761 gaaaaagtag tcgaatttat gtctgttgcc ggtttgccgg cgcgcggtgt ccgcaccaaa
     8821 ttcctagaca gctacatcaa gcgtgaaagc aaacttcaga caaacgccaa agccgacccg
     8881 cgccgctgta cccaaggttt aaactgccta accagttgcg gtctgcgcga cgggctttcc
     8941 aaagccggac aattctgcat tgatatccag cttgccgccg cattccgtgg agaagtagat
     9001 aaaggcctgt tcttcagagg taaagacccg ctgcccttcg gcaatgccat ccgcaccgtc
     9061 cgcgagacga tacaatatct gctgacgggg agcgaacctg ttgcaacgct cggacgctga
     9121 catcaggtag gatttgattt gcaaacaaaa tgccgtctga agcccttcag acggcatttt
     9181 tcaggctgca tttcggaatg gtgttaaaaa ataaacggga taagatacat ttattttcgt
     9241 ccgataaatc agaccatcac gccgaataat caaacaatgc ctgcccgaca ttgcatctgg
     9301 caaaccaatg caatgaaaac acggcttttt tatttgcttc cggcattatt gaaaagttta
     9361 tccatcgggg tcagatccac cgcattgcct tggctggtaa tccatttgtt ctcgacgcgc
     9421 cacacgcctg ccgcgtcctc cacgcgcaca taccagtggt tggtcggcga aagggttttg
     9481 aacaccgcct catattccgc cctgccgttc tgcgcgctgc cgacgggctt gagggcgacg
     9541 gtttgatcgt ccgccttgcg ggtcgggtgc atcagcagca ggttcaaagg ctgtttgccg
     9601 tcaaactcgc cgccgacaaa cacttttgcc gcattcatat cgggggaaat gagaacctgc
     9661 accccgatat gccgtctgac agcctcttca tcccgatgaa gctggatgtc gatatgtttg
     9721 ccgtctttat aataatcgtc cgtaaccaaa tctgtcgcgt gctgctgtgc gacaaaaaac
     9781 atagcgacgc tggcgatgac gacaaaaatc ggccccgcca tcaagatcca cggccagacg
     9841 tgtttgtacc aaggtttgat tggagtgttt tgagacacgg ttattctccg ataaaggttg
     9901 catcttcttc caagacgacc ggcttgccgt cgggcgcgcc gctttcgcgg tattggaagg
     9961 taaattcgat agggtggctg cctttgtccg cgtattccgg aatggtggac acttggacgg
    10021 gaagggttac cgtttcgcgc ggggcaacct tgataccgcc ttcgggcagc ccggtcaggg
    10081 cgatttcgtc aaagcctttg acacttgcgg taatgagctg ttctttttca cttttgttga
    10141 tgatacgcag gctgtatgcg ttttccagcc agcctttggc gttttcgcgc accagtacgc
    10201 cacggtcttt caaaatatcg acctcgacca ttttgcgcgt ggacaaaccg gccaggaagg
    10261 caatgataac taacgccaac accgcgccgt aacctgccac gcgcggtctg agcagccgtt
    10321 ttttaatgtc tttttcagaa tattcgtgtt ccagcgcgct ttcggtcgta taacggatta
    10381 atccgcgcgg atagcccatt ttgtccataa tctcatcgca cgcgtcgata caggcggcgc
    10441 agccgataca ttggtattgc agaccgttgc ggatgtcgat gccgacgggg cagacttgga
    10501 cgcacatcgc acagttgatg cagtcgccca aacccgcctc ttccttattg accgttttct
    10561 tgcgcgcgcc gcgcggttcg ccgcgttccg cgtcataaga aacaatcagc gtgtccttgt
    10621 cgaacatcgc gctttggaaa cgtgcatacg gacacatatg caggcatact ttttcacgca
    10681 taatgtgggc gaagaagaag gtcataaagc cgtaaaacgc tgcggcaaac atcgcgccgc
    10741 cacctgctgc tccagtgaat aaatcgggaa cgaactggcg gatagggaca aaccagcctg
    10801 caaacgtgat gcccgtccac gcgcagacaa ggaaaatcag caggtatttg gtggctttga
    10861 tgcggatttt agtgaaattc cacggcgatt tttccagttt cagccgtttg tttctatcgc
    10921 cttcgaccag gttgtcaatc cacagcataa tttcggtgta aaccgtttgc gggcaggaat
    10981 agccgcacca cagtcgccct gcaatcgtcg tccaccaaaa cagcccgaag gcgcaaatca
    11041 tcagcagcaa ggcaaggtaa atcaaatcgc ccacccccaa cgacaatccg aaaatgaaga
    11101 aatgccgttc ggggatattg aaaacgacgg cctgcctgcc gctccagttg aaccacggaa
    11161 tgacgtaaaa cacaaactgc gtcgccaata cggcggcgat acgcagtttg gcgaaccgtc
    11221 cttccgcctt tttgggatgg atgcgttcgc cttcgggatg gatttgaatc acgctggctc
    11281 gcggatcgaa tgtttttttt gctttcggcg cggcttttgt ttgttcggac gtgccgattc
    11341 cggatgccgg actgccggct tggttttccg tggtcattct gcattcctta gatttttgat
    11401 tgatggtttg cccgttaccg ccgccgtttg cttttcagac gtcatttttc ttgtttttta
    11461 aggcgttgtg tttcaagttt tgagaaaatc cgtttttccc aaaatatatt tccgctattg
    11521 tacaacttta tgcgccgtcc ggatgtatgg ggcggataca tttcccatcc gcatcaaaac
    11581 gcctggattt taccttaccg cccgaacaaa atccgaatac ggttaaaaaa aaagactaaa
    11641 aaaaccgaca cccccatatc ggcagaaccg acggcgcaag ctcataaaca aacgctatcg
    11701 acaatccggc acacaatcta taacttttta tttcaaaagg aataatggca ggcttcgccc
    11761 gcaaatcgaa aatccttccc cgcctgtccc ctgccgccgc cttcccacgc gtccgccctt
    11821 ttcttgaaag cataagcgaa tcgggcgata atcaacgctt tccgattatc cacttatctg
    11881 aaacaccagc aaggaaaata caaaatgtct caactggcaa acgcaatccg cttcctctcg
    11941 gccgatgccg ttcaaaaagc caattccggc caccccggcg cgcctatggg tatggcggaa
    12001 atggcggaaa cattgtggac gaaattcctc aatcacaacc ccgccaaccc caaattctac
    12061 aaccgcgacc gcttcgtcct ctccaacggc cacgcgtcta tgctgttgta cagcctgctg
    12121 cacctgaccg gctacaatct gagcattgaa gacttgaaaa acttccgcca gctgcacagc
    12181 aaaacccccg gccaccccga atacggctac accgacggcg tggaaaccac gaccggcccg
    12241 ttggggcaag ggattgccaa cgcggtgggt atggcattgg cagaaaaaat ccttgccgcc
    12301 gaatttaata aagacggttt gaacatcgtc gatcattaca cctacgtctt tatgggcgac
    12361 ggctgtctga tggaaggcgt atcgcacgaa gcctgttcgc tcgccggcac cttgggcttg
    12421 ggcaaactga ttgttttgta tgatgacaac aatatttcca ttgatggtaa agtggacggc
    12481 tggtttaccg aaaacatccc gcaacgcttt gaaagctacg actggcacgt cgttcccaat
    12541 gtaaacggtc atgacaccgc cgccattcaa gccgccatcg aagccgcacg tgccgaaacc
    12601 ggcaaaccgt ccatcatctg ctgcaaaacc ttaatcggca aaggcagtgc caacaaagaa
    12661 ggcagccaca aaacccacgg cgcacctttg ggcgcggacg aaatcgaagc cacgcgcaaa
    12721 catttgggct ggacttaccc cgcctttgaa atcccgcaag aaatttacga tgcgtggaat
    12781 gccaaagaac aaggcgcgaa actggaagcc gactggaacg aactgttcgc gcaatatcaa
    12841 gccaaatatc ctgccgaagc cgcagaattt gtgcgccgta tggataaaaa gctgccggac
    12901 aatttcgatg aatacgttca agccgcattg aaagaagtgt gcgccaaagc cgaaaccatc
    12961 gccacccgca aagccagcca aaacagcatc gaaatcttgg caaaagagct gcccgaactg
    13021 gtgggcggct ctgccgacct gaccccgtcc aacctgaccg actggtcaaa cagcgtttcc
    13081 gttacccgcg acaaaggcgg caactacatc cactacggcg tgcgcgagtt cggtatgggc
    13141 gcgattatga acggtttggt attgcacggc ggcgtaaaac ccttcggcgc gactttcctg
    13201 atgttcagcg aatacgagcg caacgccctg cgtatggctg cgttgatgaa aatcaaccct
    13261 gtatttgtgt tcacccacga ttccatcggt ttgggcgaag acggcccgac ccaccagccg
    13321 attgagcaaa ccgccaccct gcgcctgatt ccgaatatgg acgtatggcg gccgtgcgac
    13381 accgccgaat ccttggtcac ttgggcagaa gccgtcaaag ccgccgatca cccgtcctgc
    13441 ctgattttca gccgtcaaaa cctgaaattc caagcgcgca gcgagcaaca actgaacgac
    13501 atcaaacgcg gcggctacgt catcagcgaa gcacaaggca acgcccaagc cgtcatcatt
    13561 gccaccggtt ccgaagtcga gctggctttg gaagcgcaaa aagccctcgc cgcgcaaaac
    13621 atcgccgtgc acgtcgtttc catgccgtcc accaacgtat tcgaccgcca agacaccgcc
    13681 tatcaagccg ccgtcctgcc cgaaagcctg ccgcgcatcg ccgtagaagc cggacacgcc
    13741 gacggctggt acaaatacgt cggactgaac ggcgcagtcg tcggcatcaa ccgcttcggc
    13801 gaatccgccc ctgccgattt actcttcaaa gcattcggct ttaccgtgga caatgtggtc
    13861 gatacagtga aatcagtgct gtaaccccac tcctaaacaa atgccgtctg aacgcttttc
    13921 agacggcatt tttttgccct cccgccttga tgcttcctca ttccaccaat ctccattata
    13981 atatttgcga atcactctta ttcacatttc aaaaggagaa acgtatgaac actcgcaccg
    14041 aacacgacac catgggcaat gtcgaagtcc cgtccgaagc ctattggggc gcgcagaccc
    14101 agcgcagccg caacaatttc aaaatcggcg gcgaaaccct gccgcagccg ctgatttatg
    14161 ctttggcgtt ggtgaaaaaa gccgctgccg cgaccaatgt ttccctcggc aggattaagc
    14221 cggaacaggc ggatttgatt acgcaggcgg cggatgatgt gttgaacggc aagctcgacg
    14281 gacagtttcc tttagtggtt tggcagaccg gctccggcac gcagtccaat atgaacatga
    14341 acgaagttct ggcgaaccgc gccaacgaaa tcgccggtac gggtttggcg gcgtatcagc
    14401 ccgtccatcc caacgaccat gtgaaccacg cgcaatcgac caacgacgca ttcccgaccg
    14461 ctatccacgt tgccgccgcg attgaaatca accgccacct catccccgcc gtaaaagccc
    14521 tgcgcgacac cttggacaaa aaagcccaag ccttcgcccc tatcgtcaaa atcggccgca
    14581 cccacttgca ggacgcgacg ccgctgactt taggccaaga attttccggc tacgtttccc
    14641 agctcgacca cggcttaggc cgtctgaacg acgcgcttaa agacttgtat gaacttgctt
    14701 tgggcggtac ggcggtcggc acgggtttga acagccatcc cgaatacgcc gaaaaagccg
    14761 ccgccaaact cgccgaattg tccggcttgc cgtttgtcag cgcgccgaac aaatttgaag
    14821 ccttgggcgg acgcgatgcc gccgttgccg cttcgggcgc attgaaaacg ctggcggcaa
    14881 gcctgaataa aatcgccaac gacatccgct ggctggcaag cggcccgcgc tgcggtttgg
    14941 gcgaaatcaa aatccccgaa aacgagccgg gttcgtccat catgccgggc aaagtcaacc
    15001 cgacccaatg cgaagcgatg accatggtgt gctgccaagt gttcggcaac gacgttacca
    15061 tcggtatggc gggcgcgtcg ggcaatttcg agctgaacgt ctatatgccc gtcatcgcct
    15121 acaacctctt gcaatccatc cgcctgttgg gcgacgcgtg caacagcttc aacgaacact
    15181 gcgccgtcgg cattgaaccc gtaccggaaa aaatcgacta tttcctgcac cattccctga
    15241 tgctcgttac cgcgttaaac cgcaaaatcg gttacgaaaa cgccgccaaa gtcgccaaaa
    15301 ccgcctacaa aaacaacaaa tcgttgcgcg aaaccgccgt tgagttgggc ttgctgacgg
    15361 gcgaagaatt tgacgaactg gtcgttcctg ccgatatggt tcatccgcgc taatctttcc
    15421 ctcaaataaa atgccgtctg aaacctcgtt cggacggcat tttccgttgc ctgcgaacta
    15481 gcggcgtttg aacagcctgt cccccaccgc cgccgtaacc gcacccccga ccacgaccag
    15541 tgcgccggca taacccaaac cgttcatatc cggcgcggca aaagtatcag gcatcacata
    15601 atgcccgagc aaagaaaata ttacggtaaa cacggggagc aaggttgtta ccgcgctgac
    15661 tttggaagcc tcccaatgtt tcaacgcctc gccgaacgag ccgtaaccga ttaacgtatt
    15721 caagcagcaa tacgcaaaac aaacccacgc caacgtaccg tccaaacttc cgatgtgtgc
    15781 cagttcggca aacggcagga acacggcggc acttgccgca taaatcaaca gcagaatctg
    15841 ttgcggcccg aattgcgccg acagcagctt ttgcgccacg gcataacaca cccatgccat
    15901 actgcctacc gcacacagca acacgccctt cgcatacgcg cccaaacccg acaactcgcc
    15961 gaatttatcg ttaaaaaaca taagcaaacc ggcaagcagc aaaaccaagc cgattttctg
    16021 agcggcagtc atccggtctt taaacaccaa cacaccgaca acaatcatcg taaacggcga
    16081 aatctgccac aaaacctgcg tcgtggtcgg cgaaatataa tgcagccctt gggcaatcag
    16141 cacaaagttt gccgaaatgc ccgccacgcc gagcagcagc agcctgaatg agcaccaaga
    16201 aaaatcccgc cacttcggca gccgcccgcc caatgccagc aaaacaaaca ataccgccgc
    16261 cgccacggta aaacgcaccc acaccagcgt cggcgcatcg acaaacttca atacctgccg
    16321 cacggcaatc ggcagcgttc cccacgtcat cgccgccaaa agtgccaacg cgaagcctag
    16381 gagcggcctt tggttttcca tcctgatttt cctattttta aacaaccgta ttgccggacg
    16441 atgccggttt gccgcatcgg gcaatgatgg ttcaagcgtt tgacgtttga ttccaaccct
    16501 ttgatttcaa acaaaccggc tgaagcccga ctattgcttc gcgctatttg gaaacaccgc
    16561 ctgaatttta aaatgccgtc tgaaaacagc ggggacgtgc ggcaggcatc actgcctcaa
    16621 aacgcccgaa cggcgcaatc gcaacgttcc gcccaacgca aggggcggca acaagccggc
    16681 tcggatgcaa aaaagagaaa ccctgccccg taaggtttaa ggtttctccg tcctttatga
    16741 tttccctctg cgaggatgtc cggccgtaaa attcagaacg ggatgtcgtc gtcaatatcc
    16801 tcgaccgggg cggcggcagg cacgggttgg cggcgcggcg cggctggtgc ttcttgggga
    16861 tgggacggcg cgtcggaggc gggctgccgg ctttgctgtg cggggcgttg gtaagcctcc
    16921 tgactctgac cgtaaccttc ctcgtaaggc gcaccgccgc tgttttcatt gcgcccgccc
    16981 aacattttca tttcgttggc gacaatatcg taagcggtgc gttcgatgcc gtctttgcct
    17041 tggtatttgc ggctttggat tctgccttcc agataaacca gcccgccttt tttgaggtat
    17101 tgcccggcaa tttccgccag tttgcggtac atcgtgatgt tgtgccactc agtacgctct
    17161 acacgttggc cgttgcggtc gttccaagtt tcgctggtgg cgacgctgaa attacacacc
    17221 gcctcgccgt tgggcatata gcgcacttcg ggatcgcgtc cgaggcggcc gataaggata
    17281 actttgttca atgacatttt ttaaactcct gtgatgattt tttcagcggc agcctgatcg
    17341 aaacccttct gcaacacttt gagatagacg gtctgcccgt cgaaactgaa accgatgtct
    17401 tccacaccct caagctccga caaggcgcgg tataacactt cctgattgac ctgccacacg
    17461 ccgccgacag ggtaactgag gtttttgacg ggcttgggcg caggcgataa aacggcaatc
    17521 accagccaca gcagcatcaa tatgctgcaa aaggcaaaca cgccggaaaa gccgtatttt
    17581 tgaaacagca aaccgcctgc cacgccgccg gcaaacagtc cgagcgactg catcgtgttg
    17641 tacacgccca tcgccgtacc cttcagatcg gacggcgcga ttttggaaac catagacggc
    17701 aggctcgctt ccaacacatt aaaaccgata aagtaaacaa ccaaataagc ggtaatcaag
    17761 cctaccgagc gcataccgga cagcaaaccg agctgtgccg ccgcgatgca ggcgatgccc
    17821 aagataaaaa cctgtttgag cttgttgcgc gtctcgccga cgataatcag cggaaccatc
    17881 accaccaagc ccgtaatggt cgagggcaga tagactttcc aatgctggat tttttccaaa
    17941 ccgagctgcg tcatcgcaaa aggcagcgcg gtaaacaatg ccatttgcgc ggcgtgcagg
    18001 gcgaaaatgc cgaaatcgag cgtcagcagc ctacggtttt tcaaaacttc gcctatgcgc
    18061 gaaggctgcg cctgcgtatc ttcgtgcagc ttggaaacct ccgggtcggg agtcatccac
    18121 gccaccacgc cgatactggc aacggtcaaa ataccggtca gcagaaacaa gccgcgtacg
    18181 cccaccgcgt cggcaatcac aggggcaacg acgaggctga ccgaaaacgt cagaccgata
    18241 ctcaaaccga tcatcgccat cgcgcgggta cgtacgccgt cgcgcgtcaa atccgccagc
    18301 agcgcggtaa ccgccgcact gaccgctccc gcaccctgta tggcgcgtgc ggcgaccagc
    18361 atgggcagcg tatcggcggc ggcggcaagg aagctgcccg ctgcaaacac aatcagcccg
    18421 acataaatgg ttttcttgcg cccgaacttg tcggaagcga tgcccaaagg cagttgcagc
    18481 agagcctgtg tcagcccgta aatgcccatt gccagcccga ccagcgtttt gttgccttcc
    18541 gcgccgggca gcgaggcggc atacaccgcc aatacgggca gcacgaggaa catacccagc
    18601 atacgcagcg cgtacacgcc ggaaagcgtc gtactggcgc gccattcgtg cggaaacatt
    18661 tggatgcggt tatctcttgc catcatattt tttcagacgg catcaacagt tgcaatgccg
    18721 tctgaacttc cagtgaacag attttcggat tatacaggat tcgccgtatt tcggttgcgg
    18781 cgcgggttca aaatcaacgc cactgccagc ggttgcgcca cgcgcccaaa acggcgttcg
    18841 gatatttatt gctgcccaag ctgccgttaa agcgggcaag cgcgcggacg atgttgcctt
    18901 tttcaagatt ccggtaatgg cgcaggatgg tacagccgta acgcaggttg gtgcggatgt
    18961 cgaacaggtt gtgcgccggt ttgccgatgt agtttttcca aaacggcata acctgcatca
    19021 ggccgcgcgc gccgacaccg ctgattgcat actggcggaa cgcgctttcc acctcaatca
    19081 gccccaacac aatctgcgta tccaaaccgg cccggctgct ttcgtactgg atattgacca
    19141 gcagcctgcg ccgctcctcc tcctcgggga cgaaccttgc caaacgtgcc gacatggcag
    19201 acaaccaacg ttcgccctct ttcggattgt caaacaccag cctcggcgga ttgacgctgc
    19261 cgacagaact cctcatcacg gaagccacat cgtcggcaag cgtttcctca cgttgcgcgc
    19321 cggcgtgcgc cagaggactg agcaacaacg caccggcggc acacaacagg cggcggcgtt
    19381 gcagattaac gggtagggta tcggtcggtt ttttcatagg gaacgggggc gcgtccggac
    19441 gtttcagacg gcattaaata ttcaaacaga cataattgct ttcaatgcga aaaaccgcgc
    19501 acaaaatcca agcgcggcat atcgccctgc ccttttcggg caaaccttga ttttaccgcc
    19561 ctcaagaacg cttgtccaaa caggcacagg caacaccacc cgggcatttt ccgttttcac
    19621 cggttatccg tcgtccggat tatgcagcag caccatcagc gcatcacgct tttcgggcgg
    19681 cagcaggcgg agacctttgc aaaaatagtc tgtta
//
LOCUS       DAWFIT010000034        19651 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFIT010000034 DAWFIT010000000
VERSION     DAWFIT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.034"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(58..1227)
                     /locus_tag="WGQ85_001101"
     CDS             complement(58..1227)
                     /locus_tag="WGQ85_001101"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0750534.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1410..1520)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1573..2469
                     /locus_tag="WGQ85_001102"
     CDS             1573..2469
                     /locus_tag="WGQ85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0750535.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2513..3577)
                     /gene="tsaD"
                     /locus_tag="WGQ85_001103"
     CDS             complement(2513..3577)
                     /gene="tsaD"
                     /locus_tag="WGQ85_001103"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0750536.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3693..4880)
                     /gene="ccsB"
                     /locus_tag="WGQ85_001104"
     CDS             complement(3693..4880)
                     /gene="ccsB"
                     /locus_tag="WGQ85_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0750537.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4873..6825)
                     /locus_tag="WGQ85_001105"
     CDS             complement(4873..6825)
                     /locus_tag="WGQ85_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0750538.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINRKDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGDGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7102..7725)
                     /locus_tag="WGQ85_001106"
     CDS             complement(7102..7725)
                     /locus_tag="WGQ85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0750539.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7931..8560
                     /locus_tag="WGQ85_001107"
     CDS             7931..8560
                     /locus_tag="WGQ85_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0750540.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8681..11077)
                     /locus_tag="WGQ85_001108"
     CDS             complement(8681..11077)
                     /locus_tag="WGQ85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0750541.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11229..12344
                     /locus_tag="WGQ85_001109"
     CDS             11229..12344
                     /locus_tag="WGQ85_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0750542.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12347..12946
                     /locus_tag="WGQ85_001110"
     CDS             12347..12946
                     /locus_tag="WGQ85_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0750543.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12947..13594
                     /locus_tag="WGQ85_001111"
     CDS             12947..13594
                     /locus_tag="WGQ85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0750544.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13612..14157
                     /locus_tag="WGQ85_001112"
     CDS             13612..14157
                     /locus_tag="WGQ85_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0750545.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14176..16461
                     /locus_tag="WGQ85_001113"
     CDS             14176..16461
                     /locus_tag="WGQ85_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0750546.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQTAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17639..18151
                     /locus_tag="WGQ85_001114"
     CDS             17639..18151
                     /locus_tag="WGQ85_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0750547.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18231..19310
                     /gene="aroB"
                     /locus_tag="WGQ85_001115"
     CDS             18231..19310
                     /gene="aroB"
                     /locus_tag="WGQ85_001115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0750548.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5249 a         5607 c         4508 g         4287 t
ORIGIN      
        1 ctatataaaa atgccgtctg aactgttcag acggcatttt tgattttcaa accggaatta
       61 cagccccgct gccgccctca atgcagcagc tttgtcggtg cgctcccaag tgaactcagg
      121 ttcttcgcgg ccgaaatgtc cgtaagcggc ggatttactg taaatcgggc gcaagagatc
      181 gagcatttgg acgatgcctt tggggcgcag gtcgaaatgt tcgcgaacta aggtaatcag
      241 tttttcttcg ctgattttgc ctgtgccgaa agtatcgatg gaaatcgaag tcggttcggc
      301 aacgccgatg gcgtaggaaa cttgaatttg gcattgggtt gccaagcctg cggcgacgat
      361 gtttttcgcc acataacggc aggcgtaggc ggcggaacga tccactttgg acgggtcttt
      421 gccagagaat gcgccgccgc cgtgcggagc cgcgccgccg taggtatcga cgatgatttt
      481 acggccggtc aaaccgcagt cgccttgcgg gccgccgatg acgaaacggc cggtcgggtt
      541 gatcaggtat ttggtttcgt cggtcagcag ttcagacggc aaaacgggct tgataatctg
      601 ttcgatgacg gcgtttttca gctcttcata accgatttcc gggtcatgtt gcgtagagag
      661 gacgactgta tcaatgcgtt ttaccttacc ggtttcgctg tcgtaaacca cggtcaactg
      721 cgcttttgcg tcgggacgca gccacggcag gcggccgtct ttacgcaatt cgctttggcg
      781 ttgcatcagg cggtggctgt aatagatggc aaacggcatc agggtcgggg tttcgtcgca
      841 ggcataaccg aacatcaaac cttggtcgcc tgcgccttgg ttcaagtcga taccttcgcc
      901 ttcgtttacg ccttgggcga tgtcggggga ttgttggtcg tagtacacgc cgaccgcgca
      961 gccgttggca tcaaagccca gctcggagga gttgtagccg atgcgtttga tggtttcgcg
     1021 tgcgactttg atgtagtcca cttgggcggt ggtggtgatt tcgcctgcca atacgcacaa
     1081 gcctgtgttg accaaagttt ctgcggcgac acgcgctttg gggtcttgcg ccaagatggc
     1141 atccaaaatc gcatcggata cttggtcggc aactttgtcc ggatggcctt cggataccga
     1201 ttcggaagta aacagatatt cgctcattga gattttcctt gaaaaacaaa ccatcttctt
     1261 cagacggcat gttgtatgaa cataatgtcg acagcgggaa atatagcaaa atttccctat
     1321 tcataccatt cagttgagaa atattcccat ttgaatggca ctttggaatc tctgcccgta
     1381 cgtttcttac aggcaaaaaa attcccgcat caagcgggtt tggattgctc tggtgagcca
     1441 catcggcttt tcaaccgtcc accttacttt tccttttgaa aagcaggttg gcatggaatt
     1501 cccaactctt aatgcagcgc ggatggtagc agaaaagcct ttccgccgca atacttccct
     1561 ttttcagacg gcatgtttcg tttacaattc gggctgtttc cccctttgcg aaccgccatg
     1621 cacatcctgt tgaccgccct gctcaaatgc ctctccctgc tgccactttc ctgtctgcac
     1681 acgctgggaa accggctcgg acatctggcg ttttaccttt taaaggaaga ccgcgcgcgc
     1741 atcgtcgcca atatgcgtca ggcaggcatg aatcccgacc ccaaaacagt caaagccgtt
     1801 tttgcggaaa cggcaaaagg cggtttggaa cttgcccccg cgtttttcag aaaaccggaa
     1861 gacatagaaa caatgttcaa agcggtacac ggctgggaac atgtgcagca ggctttggac
     1921 aaacacgaag ggctgctatt catcacgccg cacatcggca gctacgattt gggcggacgc
     1981 tacatcagcc agcagcttcc gttcccgctg accgccatgt acaaaccgcc gaaaatcaaa
     2041 gcgatagaca aaatcatgca ggcgggcagg gttcgcggca aaggaaaaac cgcgcctacc
     2101 agcatacaag gggtcaaaca aatcatcaaa gccctgcgtt cgggcgaagc aaccatcgtc
     2161 ctgcccgacc acgtcccctc ccctcaagaa ggcggggaag gcgtatgggt ggatttcttc
     2221 ggcaaacctg cctataccat gacgctggcg gcaaaattgg cacacgtcaa aggcgtgaaa
     2281 accctgtttt tctgctgcga acgcctgcct ggcggacaag gtttcgattt gcacatccgc
     2341 cccgtccaag gggaattgaa cggcgacaaa gcccatgatg ccgccgtgtt caaccgcaat
     2401 gccgaatatt ggatacgccg ttttccgacg cagtatctgt ttatgtacaa ccgctacaaa
     2461 atgccgtaac gaaaataaaa atgccgtctg aacaatttca gacggcattt tgtcatctga
     2521 cgatttccga cagcggccag cgcggacgga cattgaacgc accgacctcc ctgcccttgc
     2581 ctaggtgcat cgcaccggca aaggcaatca tggcaccgtt gtccgtgcaa tatgccgtcg
     2641 gcgggaaaaa cacgctgact ttttcggacg gatgtttcgg cttgcctttg ggggtcggga
     2701 tttgcaccgt catattgccg aaagtttcac ggagcttgcg gtttgcaccg accccgccgg
     2761 cgaccactac ggttctgaac cctgtctgca acagggcttt tttcactttc gccgccaaca
     2821 catcgactac cgcatcttga aacgcacggc agatgtcgtt gcgtgtctgc tcaggaatgt
     2881 catccgcccc gttttccgcg cgcactttct cgacggcggt caatacggcg gttttcaaac
     2941 ctgaaaaact catctgcaaa tcgtcggaat gaatcatcgg gcgcggaaaa acaaacgctt
     3001 cgggcctgcc cgattccgca agttccgaca gtttcgcacc gcccggatac ggcaagccca
     3061 gcagtttcgc cgttttgtcg aatgcctcgc ccgccgcatc atcgacgctc tcgcccaaaa
     3121 gcgcgtagtc gcctatgccc ctgaccgcca taatctgcgt atgcccgccc gaaaccaaca
     3181 gcgcgacaaa aggaaagtcg ggtttttcct ccgccaacag cggcgacagc agatgtcctt
     3241 ccaaatgatg gacggggatg acgggcttgc ccaatgccaa agccagtgca ttggcgtagc
     3301 tcgaacccgc cagcagcgca ccgcccaaac caggcccctg cgtaaaggca accgcgtcaa
     3361 tgtcgccata cgatgcgcct gcctgcgcca aacagccttc agtcaacgga acaaggcggc
     3421 ggatatggtc gcggcttgcc aattccggca caaccccacc gtattcggcg tgcattgcca
     3481 tttgagtgtg caggcagtgc gctcgcaatc ctcgttccgt atcgtaaagc gcaacgccgg
     3541 tttcgtcgca agaagactcg attcctaata ccaacatggt ctgatgccgt taaaaactga
     3601 aaaacgtatt ttagcggatt tcggcacgac tgccgtatcc caaaaacgga acatgccgtc
     3661 tgaagccctt cagacggcat cgtcgcaccg tatcaaagcg ttccgtaaga atgcagcccg
     3721 ctcaaaaaca tattcacgcc gataaaagca aatgcggtta cgaacaaacc gataatcgcc
     3781 caccacgcca gcactttgcc gcgccaaccg gcaaccagcc gcaagtgcag ccaaacggcg
     3841 taattgagcc agacgatgaa cgcccacgtc tctttcggat cccaactcca atagcgtccc
     3901 caagcatctg ccgcccacag cgcacccaaa atggtggcaa tggtaaagaa cagaaagccg
     3961 acggcaatcg ccttatacat cacctcgtcg atcaatgccg acggcggcag ccacagtttt
     4021 ccgccttttc cttccgcacg cagggaaacc agttcggcaa taccgagcat cgcggaaatg
     4081 caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga ttttcatcca ccaagactgg
     4141 agcgcgggaa tcagcggctg gatggtatgc gcctcgcggg acacgctgta ccacaagaca
     4201 aatccaacca cgaccgccat aaagccgaac acgaagccgc ccaatttctg tatggcgaac
     4261 ttaccttcat aataaagata catcagcgcg gtaatcacca aaaacaggat gaacacttca
     4321 taaaggttgg aaaccggaat atgccccgca tcgggacgga gcagatagct ttcgtgccaa
     4381 cgtaccagca gaccggtaaa gcctgctacg gcagacaccc atgcaaacac ggttcccata
     4441 cccaacagcg tgttggtcgg cacatttttt acgcttgcca aaaccgcgcc cgaaatatag
     4501 gcgaacaggg cgaaaaagac aaaggcgcac tgccacataa tcgccgactg gctgctgagg
     4561 aaataccgca acaggaaaat ctctgccgat ttgatgttgc cgccatacag accgacggcg
     4621 gcataggcaa gcaatacgct taaaggaaca aaccagcgca tcggtttgaa aaaccaaccc
     4681 aaaaacacgg caataccggc actcgcccac aacatgaccg tttcgtaaat gtccatatgc
     4741 ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa ccagcacggc aaatacccaa
     4801 tcccaaagat tcagattgct gatcaaagac ttctgaatca gcagctcgtg ttccggaagg
     4861 gttttatagt gttcagtcat gattcaagtc cttgccgagc cgttgcagac tctcgacgtg
     4921 ttttggaaat tccttctgca aatcccgttc gctgcgggcc gaagacatgg caaaacggat
     4981 tttgccgtct gaaaacaata cccacgcccg tttttcgcgc acataaaaca tcaataccgt
     5041 acccaatacc aacagcaccg agccgagata gaccaaaagc gcacccgggg aacgggtcat
     5101 ctgcaaaccc gacgaacgca cctcggaaaa cccgtcaagc tggagcagca tgggcgcggg
     5161 atattcggtc aaacccgtgt acgcatccat actgtgcagc aggaaacggt tccgcgcttc
     5221 atcctgctgc cattccggca agccgtaccg gcgtatggtt tcatccaaag cagcgttcat
     5281 cacgccgtaa agcatttcgt agaaatagcc ctgcatctta tcctgctgct ctttcgggat
     5341 attggacgta ataaattcgt ccaatcccaa atagcctttt tgtgcaaaga tgttcagcgt
     5401 gttttccgca gccagcatga attgttcgcg gatttcggca ggtgcgcctt tggttgcgtc
     5461 ggcaaccaga cgtttgcgcc cgtccccatc tttcaaaaac tcacgcaacg ccataaaggt
     5521 gtccgctttc aactgcttgt ccaaggggat acgcagccag cggtattgct gctgcaagcc
     5581 gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc aaaaccggca gcatatagtt
     5641 tttatattcg accgcctgcc ctgccgcatc acggatacgg taaacaatgg aagggccgat
     5701 attggtgtat tttttacctt cctgagtaac ggcgcggaca tcgttcagcg tggatttcag
     5761 gcttttttcc cgttccgcgc cctcgctcat gtcctccaca ttcatagaag tgaactgatc
     5821 gaactcaaga cgatatttgt gtttgccaat ttccaacgga aactggtgta tggatgttgc
     5881 cttcaacacg acaggctcgc gcgaagcatc acccaaattc cacgccttga atgtcaaatc
     5941 cgaaccgccg tcggcaaaac tcgcctgata aatcgtgatg ccgtgcaagg tcaaaggatg
     6001 gttcacgcgg atggtgcgct cgagtttctc accggttgcc ttgtccgtca cttcaatatc
     6061 gctggcgaaa tcgcgcggca taccggtatt gtaaaaatcg atatggaatt ttttcagttt
     6121 gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg gcattcagga aaaccacatc
     6181 cgcactctgc ccctcggaaa tattgacgtt gcccctaaat gagagattgg acgcacccaa
     6241 aatactttcg ggcttgaaat ccttggcata aaccgcctga ttgtccggaa caatccgacc
     6301 ggtcagcata cccagtttca acagcaggtt actgtctatc aacccgccca ggcaaatgac
     6361 aatcaaagca acatgggcaa agatatagcc ccatttgttc attgtgcctt ttttggcggc
     6421 aatcagaacc gacccgtctt tacggttaat ggtttttccc tgaaaacctt gtacttccag
     6481 ataacgtttg gcaacctcgg gcgcaatttt tacatccaac agcgaagaat ggcgcatcgc
     6541 cgccagagat ttttctttaa ccttttcccg aaaagacttc atttcgcgcc agaacggcgg
     6601 cacattgcga atcaggcaca aactggtaga aaccaccaaa aacatcatga taacgacaaa
     6661 ccatgccgaa gcatagacgt catacagtcc cagaaaacca aaaatctgcg cccaaaaagg
     6721 tccgaatttg accaaataat ccgtctgcgg ctggttttgc tgcaacaccg taccgataac
     6781 cgatgcaata cccagcagac tgagcaaagc gaccgcaaag cgcatggagc tgaaaaaagc
     6841 gaaccacgga cgggaaagaa gtgggggaga tctacgggat ttactcattg tgtgtttatt
     6901 ccgccatcag gaatatggga aagcagaatt gggcaaacag aaaacaacgt cccgattcta
     6961 ctgtcttgat gctttttatt tcaagacaat gaagacagcc tgcatcgatt ccaacggttg
     7021 cgattgaaaa aacttatcgc agaattgcct gaagccgtct gaaaactttt cagacggcct
     7081 ctaaaacaga ctattgcgga attaacgcaa accttggata aagttggcga ccgctttcaa
     7141 atcttcttca gacatacggt ttgcaatatc ttccatgatg gtatttttac gctgaccgga
     7201 cttgtaggca ttcatctgtt caacaatata tgcctgatgc tgaccgccca aacgcggata
     7261 agcctgaatt tcgcttccgc ctcccggcat acccgcaccg ctcggaccgt ggcaggacat
     7321 acacgccggc acttttttat cgctcaaacc gccgcgatag attttcgcac ccaattcggg
     7381 attttcctta ggattggctt caccggattt gggctgctgt ttggcataga atgcggatac
     7441 gttcaaaata tcctgatcgc tcaaattcat taccaccggt ttcatcacag ctgccgaacc
     7501 gtgggtgcgt ttaccgtcgc ggatgccgat ggtttgatga tagatgtaag cagtatgctg
     7561 tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg tctgctgcat ggcaagccgc
     7621 acaaaccgtt gcggcaacct gtttgccttt ttccacgtct gctttgggag atgcggaaac
     7681 cgcaccggca gccaaaacaa aggccaataa agtcaatcgt ttcatggagt gctcctgatt
     7741 acagcattgg ataacgcaac aatgctcttt ttatattcaa atacgggatt tttgacccga
     7801 ttaaaaccga tgattctgca aacgtgttat tctatactaa atttacatta aattaccact
     7861 gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga caaacggcgg cggaaaacaa
     7921 ggatatgccc atgaaccttt ttcaaaacgc caaattcttc acgacggtaa accatctcaa
     7981 agacctgccg gacacaccgc tcgaaattgc ctttgtcggc aggagcaatg ccggaaaatc
     8041 cagtgccatc aataccctga ccaaccatgt ccgtcttgcc tacgtttcaa aaacacccgg
     8101 acggacgcag catatcaact tcttcgagct gcagaacggc aattttatgg tcgatttgcc
     8161 cggctacggt tatgcccaag tccccgaagc agtacgcgca cattgggtca atctgctcgg
     8221 cgactatctg caacagcgca aacagcttat cgggctggtt ttgattatgg atgcccgcca
     8281 tcctttaaaa gaactcgaca tccgtatgct ggactttttc cacacgaccg gcagaccggt
     8341 tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac gaacagataa aaaccctgtc
     8401 ccaagtcaaa aaactgctca aaccttattc cgacaggcaa aacatcagcg tacagctgtt
     8461 ttccagcctg aaaaaacaag gtattgacga ggccaaccga actgtcggaa gctggtttga
     8521 cgcagcagat gccgccgctt cctctccaaa ggaaaactga ccccaattat acggaaaccg
     8581 tattcccccc acttgaccga ccgcaaacat ttaaaaaatt gccactgcca aatctaaaat
     8641 gccgtctgaa agacttttca gacggcattt tgcggagtct ttaaaacaga gaatccaact
     8701 gctgctgttt ggaaccagta ttactcggaa gcaccggcgt ttcctgcata tcttggcgga
     8761 cttcgtcatc cgccgcctgc cgtccgcctt ctgccgcgcc cccgtcatct tcttttgccc
     8821 gtcgggaagg ttgcggcgca ataccgctgt tgtccagcgt caagcccgga tcggttacca
     8881 tacgttcctt catatagtat tcgccattgc tgctgaccac accttcaggc attttcatcc
     8941 ccttgccctg ctttcctttc aacgcaaaac gcatatagtc cacccaaacc ggcaccgcaa
     9001 tcgtaccgcc gtagccgaca cgccccatac tcttaggttt gtcgaagccg atatatacgg
     9061 cagtaaccac atcagggtta aaaccgacaa accacgcatc cttattgtca ttggtcgtac
     9121 ccgttttacc ggcaatatcc gttcttccca acgcagctgc cccccttgcc gtaccaacac
     9181 ggaccacatc ctgcataatc ttatacataa tataggcatt gcgcggatcg attgcctgag
     9241 gcgcattttg cccggccacc aaaggttgca tttgggcgcg caacctgccg tctctgtcat
     9301 aaatcttatc gattacgtgc gaagaaaccc tatatccgcc gttcgcaaat acgctatatg
     9361 cctccgccac tttcaacggc gttgtctcgc ccgtacctaa agccatagac aggcttgccg
     9421 gcagctcgga cgacctgaag ccgaaacgcc ggatatactg ttgcgcgtaa ccgacaccga
     9481 tagacatcaa aatacggatg gaaaccatat tcttggaagc cgtcagagcc tgtctcaaag
     9541 taatgtagcc ggaatatctg ccgtctgaat ttttaggtgt ccaaaccgaa ccgttcggcc
     9601 ctttccccgg cagggaaatc ggcgcatcgt taaccactgt ggacgcggtc atccccttag
     9661 ataatgccgc cgaatagaca aacggcttaa aggtcgaacc cggctgccgc attgcctgaa
     9721 cggcacgatt gaatgttttg ctgtgaaaat cataaccgcc gaccagcgcg cgcacagctc
     9781 cggtttttgc atccagcgaa accaaagccc cctgcagcaa cggctcttga accaccgccc
     9841 aacgcccgcc gttgtttttg acacggatga ccgcgcccct gcggatacgg tcctccccca
     9901 ttttttcatt attgaccgcg cgggccgcaa aacccaaggc gcgcctgtca agcgtaaccc
     9961 gcctgccgcc gggcagctgt atgacgacat ttttcttttt agtcacatcc aacacaacgg
    10021 cgggaaccat tttatcgacg gtatagagtc ccgacagata ctggctgaca gtctcctcga
    10081 catcttcact cttactcaaa tcgatatagt tttccgcacc gcggtagctg ctgccgcgat
    10141 cgaaattccg tagagccttg cgcaatgcct cggttgccac cttctgatga tcggcgcgga
    10201 ccgtggtata aaccttaaaa ccctgcgtat aggcatcttc accgtatttc tcatacagtt
    10261 cctgacgcac catttccgcc acatataaag cactctgatc gattttccga acaaaccgct
    10321 cgtaatgcag ttcctcattc aacgcctgat cgcgctgttg cacggtaatc atcttctcct
    10381 cgagcatatt gttcaaaata tacttctggc gcaacttggc acgttctgga ttaacaatcg
    10441 gattataggc agacggagcc ttgggcagtc ccgcaagcat ggcggcttcc gccaaagtca
    10501 aatctcggac attcttattg aaatagattt gcgcggcaga tgcaaaacca taggcgcgct
    10561 gaccgaggta aatctgattg aaatacaact cgaggatttt gtctttgctt aaagactgct
    10621 cgattttata ggcaagcaac acctcattga atttgcgtgt gaacgttttt tcactgctca
    10681 aataaaaatt tttcgccacc tgctgcgtaa tcgtactcgc acccgactgc acgctgccgg
    10741 acacgacatt gccgacggca gcgcgggcaa caccccaaac atccaccccc caatgccggt
    10801 aaaagcgttt atcctcggcg gcgataaccg cattccgcaa cacctctggg aaatcgccga
    10861 tttttgtaaa ttcgcgccgc tgctccccat acataccgat gacttcccca tccgccgaat
    10921 aaatagtcaa cggcatttta ggctggtaat gctgcaaaga atccaaagac ggcagtttcg
    10981 gatacgttac caaaatagca atggcaacca aacccactcc aaatacacaa aacccaaaaa
    11041 ccaaaccaaa acaagtcgtt aaaatctttt taatcatagc tgaataataa tttaccatta
    11101 ttggtattaa ataaagtaaa atagcaaccg atttctacaa agcacggttt caatgtgcaa
    11161 agaacaagga atccattacg gataccgaaa cggttactca ctgtacaaat aaagcaggaa
    11221 ctttcatcat gcgcttgttt aaaagcttga aaaaccctaa aaaaacagat gccaagctcc
    11281 ctaaaaaatc ttcgggactc aataaccgcg cggcaatcgg catcgatatc gaccagcatt
    11341 ccatcaaaat ggtccaattg tcaggacgta gtttaaacca aattcaattg gaaaaatacg
    11401 tcattgccaa attaccaaag aatatcattc aaggcaataa agtccaaaat tacgatcaac
    11461 ttgttacata tttgcaacaa gcctatgcca aactgggtac ttcgtgcaaa aacatcatcg
    11521 cgtccgtccc gcaaaatctg gcaaccatcg aacaattgac ctacacagac aaagatgcag
    11581 aattagacct gcaggggttc gtggagtcct ccatctccga agtcagctcg atatcgctcg
    11641 aagaagccaa ttacgactat caggtcttgt cccaatcggc cgccggcgaa gctgtgttgg
    11701 ccgtcgcatc gagaaaggat gaaatcgaac ccctgattga cgcattcaac gccgccggta
    11761 tgaaattatc cgcgcttgat gtggacattt tcggacaata caacgcctac gcgctatgga
    11821 taaaccattt cgcccccgag cttgcagccg aaaaagtcgc cattttcggc gtatatgccg
    11881 cacagaccta cgccttggtc atccaagacg gaaaaatcct atacaaacag gaaacctccg
    11941 tcagcgaaga acagctcaac caactcatcc agcgcaccta tcaggtaaca gaagaaaaag
    12001 cggaagaaat catcaactcc ccgcaaaaac cttccgatta ccaagaaagc gtggcaaact
    12061 atttcaacca gcagattacc caagaaatac aaagggtctt gcagttttat tacaccacgc
    12121 agaccgcaga cgatatgacc gacatcaagc atatcctgct gaccggggaa gcggcgcgcc
    12181 aggaaggcat cgcccaaacc gtcgcctcac aaaccaatgc agatgtacaa tgcgtccatc
    12241 ccgcgcgtta ttttgcggac aacctcaaaa cagacaaaca acaattcgaa cttgatgcgc
    12301 cgacactgac cagggcgttc ggtttggcgg tacggggatt ataattatga acaatttaat
    12361 caaaatcaac ctcctcccct acagggaaga gatgaacaag cgcaaacagc agcagtttaa
    12421 aacgctgatg tacggtgccg tgctgacggg cgttgccgcc gttgcggcaa cctacctgtt
    12481 tatcgacaat atgatcaata accagtcgga aagaaacacg ctgctggaaa cctccatcgc
    12541 acacttggat accgagctgt cggaaataca aaagctcaaa caggaaaaag atgccttcct
    12601 gattaagaaa aacaaaatcg aggagctcca gctcaaacgc ctccaagccg caaaaatcct
    12661 cgacagcctg aatgaggccg tccccggaag cacctacctg acctcgctgg atgccgttac
    12721 cgccgactct tatcggctca gcggcaggac atccagcgac aaccgcgttg ccgccatgat
    12781 gagggcgatg cccaataccg gcatattcaa gcaacccgaa ttgttaagca tcaagaaaaa
    12841 caattcgcat caagaattta cccttcaggc aacattacaa cccatcgtaa aggcggccga
    12901 atccaaagag aatccggctt cgggaaacgc acaggaggca aactgaatgg cttctaaatc
    12961 atctaaaacc aacttggatc tcaacaacct tcacctgctc aaccttcctg ccaggctttt
    13021 tatcgccctg ctggccgttg ccgccgtgct ggggctcggt tatgccggat tgttcaaaag
    13081 ccagatggaa tcccttgagg aatacgaagc aaaagaaacc gaactgaaaa acacctacaa
    13141 acagaaaagt atcgacgcgg ccagcctgaa caacctgagg gacgaacttg cctcaatccg
    13201 ctctgccttc gatatcatgt tgaaacagct gccgacagat gcagaaattc ccaacctggt
    13261 tcaagagctt catcaggcag gttcgagcaa cggcctgcgc ttggacagcg ttatgcccca
    13321 acctcccgta gatgacggcc ccatcaaaag attaccctat tccatttcca ttaccggaaa
    13381 ttacgaacag atcagccaat ttacccgcga tgtcggcagc ctctcccgaa tcattaccct
    13441 tgagtcgctg aaaatcgccc aatctccgga aaacggcggc aatcctgacg gcaagagcag
    13501 catcctgaac ctcagtgcca ttgccaccac ctaccaagca aaatccgtag aagagcttgc
    13561 cgcagaagcg gcacaaaatg ccgagcaaaa ataacttacg ttagggaaac catgaaacac
    13621 tatgccttac tcatcagctt tctggctctc tccgcgtgtt cccaaggttc tgaggaccta
    13681 aacgaatgga tggcacaaac gcgacgcgaa gccaaagcag aaatcatacc tttccaagca
    13741 cctaccctgc cggttgcgcc ggtatacagc ccgccacagc ttacagggcc gaacgcattc
    13801 gacttccgcc gcatggaaac cgacaaaaaa ggggaaaatg cccccgacac caagcgtatt
    13861 aaagaaacgc tggaaaaatt cagtttggaa aatatgcgtt atgtcggcat tttgaagtct
    13921 ggacagaaag tctccggctt catcgaggct gaaggttatg tctacactgt cggtgtcggc
    13981 aactatttgg gacaaaacta cggtagaatc gaaagcatta ccgacgacag catcgtcctg
    14041 aacgagctga tagaagacag cacgggcaac tgggtttccc gtaaagcaga actgctgttg
    14101 aattcttccg acaaaaacac cgaacaagcg gcagcacctg ccgcagaaca aaattaagaa
    14161 gaggattact ccattatgaa taccaaactg acaaaaatca tttccggtct ctttgtcgca
    14221 accgccgcct ttcagacagc atcggcagga aacattacag acatcaaagt ttcctccctg
    14281 cccaacaaac agaaaatcgt caaagtcagc tttgacaaag agattgtcaa cccgaccggc
    14341 ttcgtaacct cctcaccggc ccgcatcgcc ttggactttg aacaaaccgg catttccatg
    14401 gatcaacagg tactcgaata tgccgatccc ctgttgagca aaatcagtgc cgcacaaaac
    14461 agcagccgtg cgcgtctggt tctgaatctg aacaaaccgg gccaatacaa taccgaagta
    14521 cgcgggaaca aagtttggat attcattaac gaatcggacg ataccgtgtc cgcccccgca
    14581 cgccccgccg taaaagccgc gcctgccgca ccggcaaaac aacaggctgc cgcaccgtct
    14641 accaagtccg cagtatccgt atccaaaccc tttaccccgg caaaacaaca ggctgccgca
    14701 ccgtttaccg agtccgtagt atccgtatcc gcaccgttca gcccggcaaa acaacaggcg
    14761 gcggcatcag caaaacaaca gacggcagca ccagcaaaac aacagacggc agcaccagca
    14821 aaacaaacca atatcgattt ccgcaaagac ggcaaaaatg ccggcattat cgaattggct
    14881 gcattgggct ttgccgggca gcccgacatc agccaacagc acgaccacat catcgttacg
    14941 ctgaaaaacc ataccctgcc gaccacgctc caacgcagtt tggatgtggc agactttaaa
    15001 acaccggttc aaaaggttac gctgaaacgc ctcaataacg acacccagct gattatcaca
    15061 acagccggca actgggaact cgtcaacaaa tccgccgcgc ccggatactt taccttccaa
    15121 gtcctgccga aaaaacaaaa cctcgagtca ggcggcgtga acaatgcgcc caaaaccttc
    15181 acaggccgga aaatctccct tgacttccaa gatgtcgaaa tccgcaccat cctgcagatt
    15241 ttggcaaaag aatccgggat gaacattgtt gccagcgact ccgtcaacgg caaaatgacc
    15301 ctctccctca aagacgtacc ttgggatcag gctttggatt tggttatgca ggcacgcaac
    15361 ctcgatatgc gccaacaagg gaacatcgtc aacatcgcgc cccgcgacga gctgcttgcc
    15421 aaagacaaag ccttcttaca ggcggaaaaa gacattgccg atctgggcgc gctgtattca
    15481 caaaacttcc aattgaaata caaaaatgtg gaagaattcc gcagcatcct gcgtttggac
    15541 aatgccgaca caaccggaaa ccgcaatacg cttgtcagcg gcaggggcag cgtgctgatc
    15601 gatcccgcca ccaataccct gattgttacc gatacccgca gcgtcatcga aaaattccgc
    15661 aaactgattg acgaattgga cgtacccgcg caacaagtga tgattgaggc gcgtatcgtc
    15721 gaagcggcag acggcttctc gcgcgatttg ggcgttaaat tcggcgcgac aggcaagaaa
    15781 aagctgaaaa atgatacaag cgcattcggc tggggggtaa actccggctt cggcggcgac
    15841 gataaatggg gggccgaaac caaaatcaac ctgccgatta ccgctgccgc aaacagcatt
    15901 tcgctggtgc gcgcgatttc ctccggtgcc ttgaatttgg aattgtccgc atccgaatcg
    15961 ctttcaaaaa ccaaaacgct tgccaatccg cgcgtgctga cccaaaaccg caaagaggcc
    16021 aaaatcgaat ccggttacga aattcctttc accgtaacct caatcgcgaa cggcggcagc
    16081 agcacgaaca cggaactcaa aaaagccgtc ttggggctga ccgttacgcc gaacatcacg
    16141 cccgacggcc aaatcattat gaccgtcaaa atcaacaagg actcgcctgc gcaatgtgcc
    16201 tccggtaatc agacgatcct gtgtatttcg accaaaaacc tgaatacgca ggctatggtt
    16261 gaaaacggcg gcacattgat tgtcggcggt atttatgaag aagacaacgg caatacgctg
    16321 accaaagtcc ccctgttggg cgacatcccc gttatcggca acctctttaa aacacgcggg
    16381 aaaaaaaccg accgccgcga actgctgatt ttcattaccc cgaggattat gggtacggcc
    16441 ggcaacagcc tgcgctattg atgcgtcaaa ataagggcat atgttttacg gcatatgccc
    16501 tttctttatg ctttttgccg cgaccgaaat gccgtcattc ccgcgcaggc agaaatccaa
    16561 cttgtccggt tccggttgtt tttcgtctcg taacttttga gccgtcattc ccgcgcaggc
    16621 agaaatccag tccgttcagt ttcggtcatt tccgataaat tcctgttgct tttcatttct
    16681 ggattcccac ttttgtggga atgacggcgg agaggttttt gttgtttcgg agaagtttct
    16741 gcaaccctag aatctcgtta tttccacaaa aaacagaaac ccgaaacagc aacttaaaac
    16801 ctcgtcattc ccgcaaaagc gggaatccgg tccgttcagt ttcggtcgtt tccgataaat
    16861 tcctgctgct tttcatttct agattcccac tttcgtggga atgacggcgg agaggttttt
    16921 gttgtttcgg agaagtttct gcaaccctag aatctcgtta tttccacaaa aaacagcaac
    16981 ccaaaacggc aacttaaaac cccgtcattc ccgcgcaggc gggaatctag gtctgtcggt
    17041 tcaggacctt atcggataaa acggtttctc caaccctgcg ttctagattc ccactttcgt
    17101 gggaatgacg ggatatgggt ttccgtgcgg acgtgttcgg atttccgcct gcgcgggaat
    17161 gacggcgaca gatgcccaac ggtctttata gtggattaac aaaaatcagg acaaggcgac
    17221 gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgtttca gcaccttaga
    17281 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
    17341 agtattgata aacatattat cttcaatata ttcaattgga taattgttta cctaagcaaa
    17401 gataattgcc ttttcctgac aaataagtga aatcaacgga ttgtcaaaac acagcctgaa
    17461 ataaaaaacc tccctgattt cttttatttt tccttaaaat cagaaaggtt cgggatggtc
    17521 gggttatttt tccaaacgta ccgctgccct gccgatttcg tataaaattc cgccgtaacc
    17581 cgacaagccc gaaccctgtc gccccgaaag gcggggtgtc aaacattaag gaattgtgat
    17641 gaaaaacttt aacggcaaac tcatcctcat cggactgatg ggcgcgggca aaaccacgct
    17701 gggccggcaa atggcgcagc ggctggatta ccgtttttac gacagcgatc acgaaatcgc
    17761 cgtagccgcc ggcgttccca tccccaccat atttgaaatg gaaggcgaac agggattccg
    17821 ttcgcgcgaa accgccatac tcaaaaagct ggttatcctg ccccatatcg tcctgtccac
    17881 cggcggcggc gcggtgttaa aagaagaaaa ccgcgccctt atccgcaaaa gcggcacggt
    17941 cgtctatctg cacgccccgc ccgaaaccct gctcgaacgc acgcgctgcg acaacagccg
    18001 tcctttgctg caagttgccg atcctttggc gaaattacgt gaactctacg ccgcacgcga
    18061 ccccgtttac cgccaaaccg ccgactttac cgtagaatcg gcaaactgcc gggaaaccgt
    18121 gcaaaccctg ctcaaacgct tatcccgata aaccggcata tgcgccgcgc ccagaaaacc
    18181 aagccgcgcc ccgcccggcg ggccggcggt tcaaacttta aggaacaaca atgaaaacac
    18241 tgaccgtaca caccccgtcc cacagctacc ccatctttat cggcaacggg ctgctgccgc
    18301 aggcaggcag cctgctcaaa ccgcatttgg gcaaacgcgc cgccatcatc gccaacgaaa
    18361 ccgtcgcccc gctctacctc ggcacgcttc agacggcatt ggatgcggca ggcgtatccc
    18421 atttcagcat catcctgccc gacggcgagg cgcacaaaaa ctggcagacg ctcaacctca
    18481 tctttgacgg gctgatgcaa aaccgcgccg aacgcaaaac cacattaatc gcactgggcg
    18541 gcggcgtgat cggcgacatg gtcggctttg ccgctgccac ctaccagcgc ggcgcaccgt
    18601 tcgttcaaat accgaccaca ctgttgagtc aagtcgactc atcggtgggc ggcaaaaccg
    18661 ccatcaacca cccgctcggc aaaaatatga tcggcgcatt ctaccagccg caggcggtgc
    18721 ttgccgactt ggacacgctg cacaccctgc ccgtccgcga attgtccgcc ggtatggcgg
    18781 aagtcatcaa atacggcgcg ctcggcgaca tcggtttttt cgaatggctc gaacaaaaca
    18841 tatccggact gatgacgctc gatcgggaaa aactcgccca agccgtgtac cgctgctgcc
    18901 aaatgaaggc agacatcgtc gcccaagacg aaaccgaaca gggcatacgc gcatggctca
    18961 acctcggaca caccttcgga cacgccattg aaaccgagat gggttacggc acttggctgc
    19021 atggagaagc catcgccgcc ggctgcgtgt tggcggcgcg tttgtccgaa caactgggca
    19081 aaacctccgc cgcagatacc gcgcggctcg ccgccctgct cgaagccgct ggactgccgt
    19141 ccgcaccgcc cgtgtttgcc tttgaaaaat ggctcgaaca tatgagccac gataaaaaag
    19201 tcagcggcgg catcatgcgc tttatcggtc tgaaccggct gggcgaagcc aacatcaccg
    19261 aaattaccga cacggacatc ctccgccgca ccctgcaacc gtatctctga tttcctctgc
    19321 cgatgtgctg ccgcgcgggt ttgacgcacg atgatgattt tccatcatct ttctccgcaa
    19381 aagcgggaat ccagtccgtt cggtttcggt cgtttccgat aaattcccgt tgcttttcat
    19441 ttctagattc ccactttcgt gggaatgacg gcggagaggt tttggttttt tccgataaat
    19501 tcttgaggca ttgaaattct atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc
    19561 agacagtaca aatagtacga aaccgattca cttggtgctt cagcacctta gagaatcgtt
    19621 ctctttgagc taaggcgagg caacgtcgta c
//
LOCUS       DAWFIT010000035        18798 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFIT010000035 DAWFIT010000000
VERSION     DAWFIT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.035"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGQ85_001116"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGQ85_001116"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0750549.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTSGLAGMAKG
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WGQ85_001117"
     CDS             complement(1906..2343)
                     /locus_tag="WGQ85_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750550.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGQ85_001118"
     CDS             complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WGQ85_001118"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0750551.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAQAAAPAPAAAPAPTAVAAPAPSAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGQ85_001119"
     CDS             complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WGQ85_001119"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0750552.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGGGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEAERFNRLNPLETAKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRSFFEVDRYNVAVAALSA
                     LADQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7532..9244
                     /locus_tag="WGQ85_001120"
     CDS             7532..9244
                     /locus_tag="WGQ85_001120"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0750553.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGSVGFAGKVYADFVTEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDAVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNA"
     gene            9307..9963
                     /locus_tag="WGQ85_001121"
     CDS             9307..9963
                     /locus_tag="WGQ85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0750554.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRRKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGQ85_001122"
     CDS             complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WGQ85_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0750555.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10421..10969)
                     /locus_tag="WGQ85_001123"
     CDS             complement(10421..10969)
                     /locus_tag="WGQ85_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0750556.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10971..11531)
                     /locus_tag="WGQ85_001124"
     CDS             complement(10971..11531)
                     /locus_tag="WGQ85_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0750557.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11859..13664
                     /locus_tag="WGQ85_001125"
     CDS             11859..13664
                     /locus_tag="WGQ85_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0750558.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13784..15268
                     /locus_tag="WGQ85_001126"
     CDS             13784..15268
                     /locus_tag="WGQ85_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0750559.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSSIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15597..17624
                     /gene="uvrB"
                     /locus_tag="WGQ85_001127"
     CDS             15597..17624
                     /gene="uvrB"
                     /locus_tag="WGQ85_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0750560.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            17692..18456
                     /locus_tag="WGQ85_001128"
                     /pseudo
     CDS             17692..18456
                     /locus_tag="WGQ85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245990.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(18621..>18798)
                     /locus_tag="WGQ85_001129"
     CDS             complement(18621..>18798)
                     /locus_tag="WGQ85_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750561.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYN
                     GIDCKSFPLFLKEC"
BASE COUNT         4709 a         4672 c         4984 g         4433 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccaccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgttcgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctca actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtctactt tgcggccttt agccatgcct gccaaaccgc
     1261 tggtcagacg ggaaaccacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggggca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ctgcttcggc tttaggggcg gcagccgcac cttcggcttc gacgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agactgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaacagc ttcgccaaga atacggtgaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca acgggcacat caggcgcggg gcaaactctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 cggcgtgaaa cctgtgccgc cgatgccgcc caagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcgcg ggcttttttg gacaattcgg tcaattcttg
     2761 gctgatttgt ttcaagcctt tttgatctac gtctttgatg acgggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaggt tgtcgccatc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccagagga gacagtttca cgccttcgcg ttcccattct ttgttcagtt gtttgcggaa
     3001 ttcttccagt tcggtcatat ccgcttcttc gtgtacggta acgtgcggaa tcacaaccca
     3061 gttgcgggac aggttttgac cggaaatttt cttaatgcgg gacaattctt taacttcgac
     3121 attaccgaat ttggagaagt ccactttagg ccagggcagc aagtccagac cgccgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc accgccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcga cagcagtcgg
     3421 agccggggcg gcagccggtg cgggtgcggc ggcttgagca ggggctgctg cggcagagcc
     3481 ggcggtttct acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     3541 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     3601 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc actttgattt caacggcgat
     3661 tacatccaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     3721 tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag gagcggcttc
     3781 ggcagcggcg gcaccggttt caacggtcag aataacgccg ccttcagaga ttttatcgcc
     3841 cactttaact ttaacttctt taacaacacc ggctgcatca gcaggtacat ccatagtggc
     3901 tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca atagtgtcgc ctactttgac
     3961 ttctacggcg atgatgtcga cgttttcatg accgccgata tcagggactt tgatttctac
     4021 gatactcatt tgagttcctt taatattttc agtttgatgc cgtctgaaaa tttgtttcac
     4081 acggcatcgg tacagtttgt tcaaaccatt caagcattcg gttttcagac ggcctgatgt
     4141 ctgaatgaag caaacaggcc gtctgaaaca tcaattagcg tttccagcta ggagctgaat
     4201 cggctttgat accatatttt tcaatggctt gttgaacggt ttctttgctg actttgcctt
     4261 gatcggccaa tgcgctcaat gcagcaacgg caacgttgta gcggtcaact tcgaagaagc
     4321 tacgcaggtt ggcgcggctg tcggagcggc cgaagccgtc agtgcccaag acgtggtaat
     4381 cgttcgggat ataggcgcgg atacggtcgg catagctgcg gatatagtcg gtagcggcaa
     4441 tcactgggcc gtcatgacct tgcagttgag aagtaacaaa cggtactttt gcagtttcta
     4501 aaggattcag gcggttgaaa cgttctgctt cgacagcgtc gcgatgcaac aggttgaaag
     4561 atgggcaaga ccaaatgtct gcttccacac cgaagtcggc tttcagcagc tcggcaccgg
     4621 caatcacttc ttgcagaatc gtaccggaac ccatcagttg gactttcttg tcgcctttgc
     4681 cgccggcttt cagcaggtac atacctttga ggatgtcttg ttccgcacct tccggcatat
     4741 ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct tcattattgg
     4801 catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg aaagtcgggt
     4861 cataagatac gcagttcgga atcaaatctg cttgaatatg gctgtggccg tcttcgtgtt
     4921 gcagaccttc gccgttcagc gtcgtacggc cggcagtacc acccaacagg aagccgcgtg
     4981 cgtgcatatc acctgccgcc caagccaagt cgccgatacg ttggaaaccg aacatagagt
     5041 agtagatgta gaaaggaatc atcgcaaagt tgctgttggc gtagctggtt gcagcagcga
     5101 tccagtcagc cattgcgccc ggttcgttaa taccttcttg caagatttga ccgtcaacgg
     5161 attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga cctttcggat
     5221 tccaaatacc gtattggcgg aacatgcctt ccataccgaa agtacggctt tcgtcgggaa
     5281 cgataggtac gacgcgtttg ccgatttttt tgtctttcaa caaggttgac aaaatgcgga
     5341 caaatgccat agtggttgag aattcgcggt cgccgctgga tttcagttgg gtatcaaatg
     5401 cggacagctc cggcacttcc aaaacttctt gggtaggttt gcgttgcggc aggtagccgc
     5461 ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca
     5521 ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc
     5581 ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg ttctgacctt
     5641 cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg gtaggtttgc
     5701 cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg tcgtggccgc
     5761 cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgct ttcagttcgg
     5821 gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt
     5881 cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct ttcgccaaga
     5941 ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg ccggcaaagt
     6001 tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc aagcgttgca
     6061 gattgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc agtgcgattg
     6121 caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat actttacggc
     6181 ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc tgataaatcg
     6241 ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg ggcaagaggt
     6301 gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag ttgttcagct
     6361 ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg gcgacgtgac
     6421 cttggaagaa gaccaaatcg ccttcttcgc ctccgccttt ggctttccaa aagtggttga
     6481 aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca cccagttcca
     6541 aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgga
     6601 tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc gaaacggtat
     6661 tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg cagtatttga
     6721 ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg acggagctta
     6781 acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg gtggacatag
     6841 tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac ccctttattt
     6901 tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga aaaaaataat
     6961 ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa acttgaaaaa
     7021 aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg caaataacca
     7081 tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag tctttttgat
     7141 ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat atcgaatcat
     7201 tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt tgtggataac
     7261 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
     7321 tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt caatatgatg
     7381 tttctggtca tgtttttgcc gtctgttatg ggatttgtcc cgcgcttggg gcagggggtg
     7441 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
     7501 tttgaatctg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
     7561 tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga ttcgcgccgg
     7621 cctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc tgcgcgtgtt
     7681 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagttgct
     7741 gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg agttttacgg
     7801 taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg gcccgacctg
     7861 cgaggaagtc atcgccgaca tcgtgcgcaa agaaatcaac agctacaaac agctgccgaa
     7921 aaacttttac cacatccaaa ccaaattccg cgacgaagtg cgcccgcgtt tcggcgtgat
     7981 gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc catgccgact acgcctcgct
     8041 tcagacgacc tatgatgcca tgtatgacgc ttactgccgc atcttcactc gtttgggttt
     8101 ggcattccgc cccgtcgccg cagacaccgg cagcatcggc ggtacgggtt cgcacgagtt
     8161 tcaagtgttg gcggaaagcg gcgaagatgt tattgcgtac agcgacactt ccgattacgc
     8221 cgccaatatc gagttggcac cgaccttgcc gcttaaaggc gaacgcgccg ctgctcaggc
     8281 tgtgttgacc aaagtacata caccaaacgt caaaaccatt gagtctttgg ttgaattcct
     8341 gaatattccg gttgaacaaa ccctcaaatc catcgtggtc gaaggcgaaa acgaaggcga
     8401 aatcgtctta ttactgttgc gcggcgacca tgagttcaac gacatcaagg cagaaaaact
     8461 ggcgggcgtg aaatcgccat tgacgatggc aagttctgcc gcgattgtcg aacagttcgg
     8521 ggcaaacggc ggctcgctgg gttcggtcgg ctttgcaggc aaagtttatg ccgatttcgt
     8581 cactgaaaaa ggcgcggact gggtcatcgg tgcaaacgaa gacggctacc actacaccgg
     8641 cttcaacttc gggcgcgacg ctgccgagcc tgaatttgtc gatttgcgca atgtcgtcga
     8701 aggtgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
     8761 acacgtcttc cagttgcgcg acaaatacac ccaagccatg aacgtgagct tcctcgacaa
     8821 caacggcaaa tcgcaaatca tggaaatggg ctgctacggt atcggcatca cccgcgtcgt
     8881 tgccgccgcc atcgagcaga acaacgacga aaaaggcatc atctggacga aggcgatggc
     8941 gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacgccg tgcgcgaagc
     9001 cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggatgaccg
     9061 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg ttgggtattc cgcaccgcat
     9121 cgtcatcggc gaccgcgctt tgaaagaagg caatgtcgaa tacgccgaac gccgcaacaa
     9181 cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt gtaacagctt cattaaatgc
     9241 gtaagcttgc tcgaatgccg tctgaaaata atttcagacg gcatttttac ttagggggaa
     9301 agtgaagtgg cttcagtatt tttagaagga gaggcagtcg aggtcggcaa tattttctgt
     9361 atcggcagga actatgccgc acatatcgaa gagttaaaaa acgaaatacc ttccgaacct
     9421 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
     9481 gagtttagta gggatgtgca gtttgagtgc gaactcgtgc tgctggtcgg taaggattca
     9541 gacggcacgg gcgagggtaa agatattttg ggatgtgtcg caggatatgg tgtagggctg
     9601 gaccttacgg cacgggatat ccaatgccgt cggaaggaaa aagggctgcc ctggctgaag
     9661 gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg cagaatcggc
     9721 aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggat
     9781 accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc agattacggt
     9841 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
     9901 ggggacaatt tggcattgga gctggacggc ttggtgcgcg cctctttcac ggtagggtgt
     9961 taaccgcgcc gcttaaccct cacgcccgtt gaaacattcc gccggaccgc cctcgaaaaa
    10021 accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgccgaa
    10081 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    10141 atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg acaggtgcgt
    10201 catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat aacgcggctg
    10261 ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc cgccggtaac
    10321 ggtgctcaaa ggatgggata gccctaattc cgcgtctcct tgtgccacgc cgatacgcgc
    10381 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    10441 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    10501 aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt cagccacgca ttgtcggcaa
    10561 gttcgcgttc gagctggcct tttccccagc ttgaatagcc tatgctgatc aaggctttgt
    10621 caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtt agcgcgatat
    10681 tgtctgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg acataacctc
    10741 gctcgacttg caccggcccg cccatcatca cgctgtcgtg ctgcatccgc atggggatgt
    10801 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    10861 tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    10921 cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata tcagcgtttg
    10981 tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt gccggaagta
    11041 tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac cgcgcttaag
    11101 gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc gaaggttttg
    11161 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct cgcaccgtgt
    11221 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    11281 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    11341 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    11401 ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact tgcccgtccg
    11461 attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga cagcagtagc
    11521 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa ggcggcaggg
    11581 cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt atgcggctta
    11641 atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat atgacgcaca
    11701 atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa gattgggtaa
    11761 acggcatttt cagacggcat aactgacagt ataatccaaa cgtccggctg ctttcgtgca
    11821 gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa ccccttctgc
    11881 ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg gcacacaacc
    11941 gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa gcggcggaag
    12001 gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc ggcaaagagg
    12061 cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa gcagagaagg
    12121 aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa tcgaaggcgg
    12181 aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac gcaaaaacac
    12241 agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca gggcaatccg cgtaagggcg
    12301 gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac aagaacggca
    12361 aagcagtgaa acaggataaa aaatacaggg aagagaaaaa tgccaaaacc gattccgacg
    12421 aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa gccctgctca
    12481 aacaaagcga aggaatgctg cttcatgtca gcaattccct caaacagctt caggaagagc
    12541 gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc aaccttgctt
    12601 ccgtcaaccg caaacagcgc gaggcttggg acaagttcca aaaactcaat accgagctga
    12661 accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc gtatcgggga
    12721 actataaaaa cagccagccg aatgcggttg ccctgttcct gaaaaacgcc gaaccgggtc
    12781 agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc ctccaatcgg gaagttgtca
    12841 aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc aacaatgagc
    12901 ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa cagggtgtaa
    12961 ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc aaagatgccc
    13021 gaaaactgct ggaacagaaa gggaacgagc agcagctgaa caagctcttg agcaatttgg
    13081 agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa ttggctgaag
    13141 ccaggctggc ggcagccgaa aaagccagaa aagaagcggc gcagcagaag gctgaagcac
    13201 gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa catccaagcg ccttcggtta
    13261 tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg acgtttgaaa aaaccggttg
    13321 acggtgtgcc gaccggactt ttcgggcaga accggagcgg cggcgatgtt tggaaaggcg
    13381 tgttctattc cactgcaccg gcaacggttg aaagcattgc gccgggaacg gtaagctatg
    13441 cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga tcacggcgag aactacatca
    13501 gcatctatgc cggtttgagc gaaatttccg tcggcaaggg ttatatggtc gcggcaggaa
    13561 gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga ggggctttac ctgcaaatac
    13621 gttatcaagg tcaggtattg aacccttcga gctggatacg ttgatcggat tgccgaaacg
    13681 gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc cgtgaagttt gccgtacctg
    13741 tattttccgg tttttggaaa tcaagagaga aagttaaaga agaatgtcga aacctgtttt
    13801 taagaaaatc gcactttata ctttgggtgc aatcagcggc gtggccgtca gtctggcggt
    13861 gcagggtttt gccgccgaga aggaccggcg ggataacgaa gtcctgccgg tgcaatccat
    13921 ccgcacaatg gcggaggttt acggtcaaat caaggcaaac tactatcagg acaaacccga
    13981 tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc ggtttggatc cgcattccga
    14041 atatatggat aaaaaaggtt atgccgagat aaaggagtcc accagcggcg aatttggcgg
    14101 cttggggatg gaaatcgggc aggaagacgg atttgtcaaa gtggtttcgt cgattgagga
    14161 cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc attgtgaaaa tcgataatgt
    14221 ttcgacgcgg gggatgacag tcagcgaagc ggtgaagaaa atgcggggca agccgggtac
    14281 gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag ccgatagtcg tcaacctgac
    14341 ccgtgccatt attaaagtga aaagcgtccg ccatcacctg atcgaacccg attacggcta
    14401 tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc gtcaataccg ccgcaaaaga
    14461 gctggtaaag gaaaataaag gaaaaccgct caaggggctg gtgttggatt tgcgcgacga
    14521 ccccggcggg cttttgaacg gcgcggtcgg cgtgtcggcg gcgtttttgc cgcctgatgc
    14581 gctcgttgtc agtacgaaag ggcgggaagg tacggaaggc tttgcgttaa aggcgcgtcc
    14641 cgaagattat atttttaaag ccggcacgga tcctttggcg ggtattcctg ccgagttgaa
    14701 aaccataccg atgacggttc tgattaattc gggttcggct tccgcgtcgg agattgtcgc
    14761 aggtgcattg caggatcata aacgcgcggt tatcgtcggt acgcagagct tcggcaaagg
    14821 ttcggttcag actttgattc ctttgtccaa cggcagcgcg gtcaagctga caacggcact
    14881 gtattatacg ccgaacgacc gttctattca ggcgcagggg attgttcccg atgtcgaagt
    14941 aaaagataag gaacgcattt ttgaaagccg cgaggcggat ttggtcggac acatcggcaa
    15001 tcctttgggc ggcgaggatg tgaacagtga aacccttgcc gtgccgcttg aaaaagatgc
    15061 ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag gacgaggatt tgtcttcaag
    15121 gcggattccc aaccctgcca aagacgacca gttgcggaaa gctttggatt tagtcaagtc
    15181 gcccgagcag tggcagaagt ctttggggct ggcggcgaaa aagccggttt caaataaaga
    15241 taagaaagat aaaaaagata agaagtaggt tttgacgatg ccgtctgaaa ggatttcaga
    15301 cggcatatta tgtttgaaag gacaggttat gagcgatttt ttaccggaat ttgaaccgtg
    15361 cggcatttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
    15421 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta tacttgggta
    15481 gcgattgggt ttcagacggc atacattcag gatttcaccg ccttatgccg tctgaaagac
    15541 gtttccgacc catccctact acacgctttg tccgcaaacg catcacaggc aagattatgg
    15601 aagtcatccg ataccccgat tcccctttca aactccacca acccttcccg cccgcaggcg
    15661 accagcctac cgctattgcc ggcttgctcg aagggctttc agacggcctt gcctaccaaa
    15721 ccctgctcgg cgtaaccggt tcgggcaaaa cctacaccat ggcgaacgtc atcgcccaaa
    15781 gcggccgacc cgccatcatc atggcgcaca acaaaaccct tgccgcccag ctttatgccg
    15841 aaatgcgcga gtttttccct gaaaacgcgg tggaatattt cgtctcctac tacgactatt
    15901 accaacccga agcctatgtg cccagccgcg acctgttcat tgaaaaagac agcgcgatca
    15961 acgaacatat cgagcagatg cgcctttccg ccaccaaaaa cctgatgacg cgcgatgacg
    16021 tgattatcgt tgccactgtg tccgccattt acggtatcgg cgacccgact gagtatcaac
    16081 aaatggtgtt gtccgtcaaa gaaggcgaca ccatcgagca gcgcgacatc atcgccacgc
    16141 tcgtttccat gcagtacgaa cgcggcgatt tggatttcaa acgcggcagc ttccgcgtgc
    16201 gcggcgacgt gattgacgtg taccccgccg aaagctccga aaacgccttg cgcatcagcc
    16261 tgtttgacga cgaaatcgac cgcctcgata tgttcgaccc gctttcaggc agcctgcacc
    16321 aacgcgtcgg ccgctacacc gtcttcccgt ccagccacta cgttaccccg cgcgacaccg
    16381 tattgcgcgc ctgtgactcc atcaaagaag aattgcgcga acgcattgat ttctttacca
    16441 aagaaaaccg tcccgtcgaa caacaacgca tcgaacagcg cacccgcttc gacctcgaaa
    16501 tgctctacga aatgggcttc tgtaaaggaa tcgaaaacta ctcccgccac ttctccggca
    16561 aaaaagaagg cgaaccgccg cccacgctga tggactacct gccaagcaac gccatcatgt
    16621 tcatcgacga aagccacgtt accgtaaccc aaatcggcgg tatgtacaaa ggcgacgcat
    16681 cgcgcaagca aaacctcgtc gattacggct tccgcctgcc ttccgcccgc gacaaccgcc
    16741 cgctcaaatt ccacgaattt gaaaaagtca tgccgcaaac cgtcttcgtt tccgccaccc
    16801 ccgccaaata cgaagaagaa cacgccggac aagtggtcga acaagtcgtc cgccccacag
    16861 ggctggtcga ccctcaaatc atcatccgcc ccgtcgccac ccaagtcgac gatttaatga
    16921 gcgaaatcaa cgaccgcatc caaaaaggcg aacgcgtact cgttaccacc cttaccaaac
    16981 gcatggcgga gcaactcacc gactattaca gcgaactcgg catcaaagtg cgctacctgc
    17041 acagcgacat cgacaccgta gagcgcgttg aaattattag agatttacgg ctcggattgt
    17101 ttgacgtact cgtcggcatc aatctcttgc gcgaaggcct cgacatccca gaagtctccc
    17161 tcgtcgccat cctcgacgca gacaaagaag gcttcctgcg ctcccaccgc agcctgattc
    17221 aaaccatagg ccgcgctgcg cgcaacgtga acggcgtcgc gattctgtac gccgacaaaa
    17281 tcaccgactc tatgaaagcc gccatcgacg aaaccgaacg ccgccgtgaa aaacaaatta
    17341 aattcaacga agaacacggc atcgtgccgc aacagattaa aaaacaggtc aaagacatca
    17401 ttgacggcgt gtaccacgaa gaagacagcg gcaaaggccg tagacaaggc aaaaacaaag
    17461 ttaaagtcgg cgaaattcac aacgaagaag acgcgattaa agaaatcgcc aaactcgaaa
    17521 aagccatgca acaggcggct agggatttgc agtttgaaga agcggcggtg ttgagggata
    17581 ggattaggaa tattaaagag aatttgttgt ttggggcaga gtaaaggagc ttgatttttt
    17641 aattagcgaa gatacaataa cgtcatctat tatttttata ggaaagtaac tatggcagag
    17701 caactacagc tgattcctcg tgttgaaagc ggtgaaatta tcccacagag aatgagtgac
    17761 ggatatatca atgcgacagc attgtgtaaa tctgtaggta aatcttacag tgattataga
    17821 caattacagt ctacaaatca tttcttaaat gaattgaaag cctagacggg attatccgaa
    17881 caacaattga ttcaacagag aattggtgga gaaccttctt tacagggctc ttgggtacac
    17941 ccttatttag caattaattt agcacagtgg ttatcccctg catttgctgt aaaggtatct
    18001 acttgggttc atgaatggat gtcaggtaaa gtcaatgctt ctccttccca ttccgcgcat
    18061 atccaaagat acttgcaaaa tagaggaaaa attccatata cgcatttttc aatgcttaat
    18121 gaaattacgc taaatttaat tgctcctttg gaggaacaag gttatgtatt acctagtcag
    18181 ttagttccgg atatttcaga aggaagatta ttttgtagat ggctgaggga aaatagaaac
    18241 gttgatccga ataacttccc aacatataaa catgaattcc ttgatgctcg gagacccgtg
    18301 gaggccaaat tatatccaat agaatactat caagatttta aatggcactt taatgaagta
    18361 tggttaaaag aaaaagctaa acaatatttt tcagaacgtg atgcagcaat tttgccttat
    18421 ttaaccccgt taatgttacc agcaagcgag tcgtagcaag ccgtagcctg cattaccaac
    18481 caccgcgtgc gtagctacgc catacaccct acctgcgggc gggacaaact ttaaggggct
    18541 gtactagatt agccctaaat cccataccaa tcccgcagga ttttaagctg ttgagacggt
    18601 gtgccgaagt taaatcgaaa ttagcattct ttcaagaaca gtggaaaaga tttgcaatcg
    18661 attccgttgt attttcgcaa gacgcgtttt gcctgattcc aaaaattctc aatgccgtta
    18721 atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg gataaaaccg
    18781 ctcacgtcca acttgtcg
//
LOCUS       DAWFIT010000036        18704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFIT010000036 DAWFIT010000000
VERSION     DAWFIT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.036"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..190)
                     /locus_tag="WGQ85_001130"
     CDS             complement(<1..190)
                     /locus_tag="WGQ85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750562.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGFMPSEGSDGIGAGKSE"
     gene            complement(208..765)
                     /locus_tag="WGQ85_001131"
     CDS             complement(208..765)
                     /locus_tag="WGQ85_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0750563.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNREVA
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(841..2475)
                     /locus_tag="WGQ85_001132"
     CDS             complement(841..2475)
                     /locus_tag="WGQ85_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0750564.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2549..2878)
                     /locus_tag="WGQ85_001133"
     CDS             complement(2549..2878)
                     /locus_tag="WGQ85_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750565.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WGQ85_001134"
     CDS             complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WGQ85_001134"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0750566.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMSDYNGTGFSVMEMSHR
                     SDMFLSILHHAEQDLRQLLNIPSNYKILFLQGGATTQFNMAVLNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4349..4780
                     /gene="rimP"
                     /locus_tag="WGQ85_001135"
     CDS             4349..4780
                     /gene="rimP"
                     /locus_tag="WGQ85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0750567.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NLIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4808..6310
                     /gene="nusA"
                     /locus_tag="WGQ85_001136"
     CDS             4808..6310
                     /gene="nusA"
                     /locus_tag="WGQ85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0750568.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6322..9210
                     /gene="infB"
                     /locus_tag="WGQ85_001137"
     CDS             6322..9210
                     /gene="infB"
                     /locus_tag="WGQ85_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0750569.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9421..10815)
                     /locus_tag="WGQ85_001138"
     CDS             complement(9421..10815)
                     /locus_tag="WGQ85_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0750570.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10889..12229)
                     /locus_tag="WGQ85_001139"
     CDS             complement(10889..12229)
                     /locus_tag="WGQ85_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0750571.1"
                     /translation="MLNPSQKLVELVRILEEGGYIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWHEGT
                     WFTGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLAEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12373..12999
                     /locus_tag="WGQ85_001140"
     CDS             12373..12999
                     /locus_tag="WGQ85_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0750572.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSI
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13063..14481)
                     /locus_tag="WGQ85_001141"
     CDS             complement(13063..14481)
                     /locus_tag="WGQ85_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0750573.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTVFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRIIYDDYL
                     MQKKARQDLSYQYEFYEFHDKG"
     gene            14808..15536
                     /locus_tag="WGQ85_001142"
     CDS             14808..15536
                     /locus_tag="WGQ85_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0750574.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKIRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVANDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15595..16083)
                     /locus_tag="WGQ85_001143"
     CDS             complement(15595..16083)
                     /locus_tag="WGQ85_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0750575.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGIEPCVLCISQRLC
                     VLATALCAVIVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPPCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGIALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16772..17236)
                     /locus_tag="WGQ85_001144"
     CDS             complement(16772..17236)
                     /locus_tag="WGQ85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0750576.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17461..18519
                     /gene="alr"
                     /locus_tag="WGQ85_001145"
     CDS             17461..18519
                     /gene="alr"
                     /locus_tag="WGQ85_001145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0750577.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIKLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         4898 a         5158 c         4741 g         3907 t
ORIGIN      
        1 cttctgattt cccggcaccg atgccgtctg aaccttcaga cggcatgaaa cctttgcctg
       61 cgtacttggt gcgctggtcg cctccgaaca tggcgcgaca cccgacattt ccgccgaacg
      121 catcgggcgt ttcatgaatc cggtttaaaa cgcatggaaa aatgccgtct gaaagccttt
      181 cagacggcat ttcgcttgag attccgttta ccaatggctg acaaacgctt ccaaatcggt
      241 attcttgggc ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc cgatgatttt
      301 atccttatcc gcacatccga aagcctcacg caacaggggg ctgttgaccc acatccccgt
      361 aatccagaca ttgtcgaaac cttgcgccgt tgccgccagt tgcagcgcat acgccgcaca
      421 acccgccgtc agcatctgct cccattccgg tttcggcttg gcgacttccc ggttgggcgc
      481 aaacgttacc ccgatgacca tcggcgccat attgcccact ttttccgcct ttttcatcgc
      541 atcgtcgccg aaattcaatt cggcaaccgt ttgctttaac acatcgcgaa aacgttgcaa
      601 tcctacctcg ccttgaatca cgataaaacg gaaggggcgc atattgccgt gatcgggaac
      661 ttgggttgcc gcctgaaata tttgttccaa ctccgccgca tcgggggcgg ggggcttcag
      721 ctttttggaa gatcggcggt tcgtcaataa ttttaaagca tccatatcgt tatactccgg
      781 tcatcggtcg ggtgttcgga cacatatgcc gtccgaaggc ttcagacggc atatccggca
      841 tcagcgcgga cggcggcagg ctgccaatat atccatttcc ttccgatagg tttgactgtt
      901 ggaaatgtcc atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc
      961 cgccgctttt tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga
     1021 aaaccacata accatcggga tatgcgtctg cccggaaggc gcgatggcgt aaggcgcggc
     1081 gtgcaggtac atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatgcaccac
     1141 gctttccaaa tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actggtccac
     1201 atacaaaacc gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt tgttgatttc
     1261 gttggtgtcg caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt cgtaatacgt
     1321 cggcccgtgg ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag
     1381 gacttcgtcg aacttagtca gcaggatatt gtcgaggcac tcgccgttgc ggcagtattc
     1441 gggcaggttg agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc
     1501 ggaatcgttt tccaaccagg taacttccac gccggcgcgc tgcacgatgt ccagcaggtt
     1561 gtcttggtgt tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca
     1621 cggcagggag tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc
     1681 atcgccgcgc gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt
     1741 ggcggcacgc gtggtctcgc ccacgaccag caccacgaaa cggcgcaggc tgccggccgg
     1801 ccggttttgc acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc
     1861 tttgtatttc gacacgcccg cgccgatgaa attagacggc acaatcagat gggttactga
     1921 tttattgttg cggaaaaacg aggcgtaatc ctgatattgc aacattgcga tgcccaacgc
     1981 gcacaaaaag gaaacggcgg caagcacaag gcgcgtcaaa agctccttat accaaacgcg
     2041 gtatttaacc ttgacggcga tatacgccag cgcgggcaat acgcccaaac atacaatcca
     2101 cagcacatag cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt gcaagacatt
     2161 attcaacatc gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc
     2221 actgataacc aatatcaacg gaatcaatac cttatgcacg aaaggcaggg caatgacgtg
     2281 aaaaacgaaa ttacttaaaa aaaacagcac caccggcatc gtatagagga agatatccgc
     2341 cccggtgccg ttaaaaggat gaagctcgac aactttggca aaaaaggcgt aattcaatac
     2401 cagcgaggaa tacagggaaa ggaaggcaat cagcgcggaa gagccgagct tcggcctcag
     2461 gttcggtttt atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggcatttgg
     2521 aatccggatt attgaaaaag attcttaatt ataaggcaac ggagcaaagc agggcaagaa
     2581 aacggcggct gtgcgggggg ttccgcccgc cattcaaacg tccggcagac ataaaaacat
     2641 cgtaagcaag attcgaaccg gtctgcaacc gcccctgcca gaaaaacggg caaagcattt
     2701 catattggaa aaacccggcc gcgccgccga cgggacagtc cggcacaaac agcatcacgc
     2761 ccggattgaa aaggacggat agccgccggc agcacaatct taccacctcc aaaacgccgc
     2821 cggagaacgc cgaaacagcc gatgccgtct gaagccgctt cagacaacat cgggacatca
     2881 accgtaacgc cgttggaaat cgcgcataaa atctgccaaa gcccgcacgc cttcaagcgg
     2941 catcgcatta taaatgctgg cacgcatacc gccgacggtt ttatagccct taagcaggca
     3001 caagccctgc aattcggctt ccagcacaaa acggcggtca agctcctcat cccccgtttg
     3061 gaacacgaca ttcattttag aacgcgcatt cggacggata cggttgatat agaaaccgcc
     3121 gctgccatct atcgtctcat acaaggtttg cgccttcagc cgattgaccg cttcaatttt
     3181 tttcacaccg ccctgcgcct gcagccagcg gaacaccagc cccgacatat aaatcgcgta
     3241 agttgacggc gtgttgtaca taccgtcgcg gttcaaatgc gaacggtagt tgaacacatc
     3301 gggaatatcg ttcggacaac gctcgagcaa atcctcacgc acaatcacca ccgtaacccc
     3361 tgccggcccg atgtttttct gtgcgcctgc gtaaatcagt ccgtagtcgg caacatcaaa
     3421 ctcacgcgac agaatctcgc tggacatatc gcacaccaac ggcggcatgc cgtctgaaag
     3481 gcgaggcact tcacggtatt gcagcccgtt gaccgtttca ttgacggcaa aatggacaaa
     3541 cgccgaatcg ggtgcaacat cccacgtttc cacaggcggc aggtcgagat agtcgaactg
     3601 ctcgccgcca tgcgccgcca aacggatttc cgtatcggtc aaacggctca tctgttcata
     3661 agcgatacgg ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac cgtgtgccag
     3721 attcaaaacc gccatattga attgcgtggt cgccccgccc tgtaaaaaca ggattttata
     3781 gttggacgga atgttcaaaa gctgccgcag gtcttgttcg gcatgatgca ggatgctcaa
     3841 aaacatatcc gaacggtggc tcatttccat cacggaaaaa cccgtaccgt tgtaatccga
     3901 catttcctgc cgcgccgttt ccaacacggc ttcgggcaat acggcagggc cggcggaaaa
     3961 attgtaaatc ggataaagag acatgatgca gccttgattc tgaacaataa cccgcctgat
     4021 tttaggcttg cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg ccccgcttca
     4081 tgccgcccga acacggaaaa caacatcaat atattgattt acaaatataa aaatcatgct
     4141 agcgacaaat agatacattt gttttgtcaa caatattcac gatttcccat tacaaacctc
     4201 ccttacaccc gcttttttcc gtcccaaaaa cacaaaataa atcaacactt tcatttctcc
     4261 gcaaaagcgg ttataatcac gccgattttt caactttgac gaaaaatggc cacggggcca
     4321 tttttttatt atgtacatag ggagcagcat ggatatccaa accatcctcg aaaaaaccct
     4381 gcccggcctg ggctacgaac tggtcgattt cgaactgacc gcgcaaggaa cattgcgcgt
     4441 gttcatcgac aaagaaagcg gcattaccgt cgaagactgc gcaaccgtca gcaaccactt
     4501 gagccgcgtc ttcatggttg aagacatcga ctacaaaaac ctggaaattt ccagccccgg
     4561 actcgaccgc cccttgaaaa aagccgccga cttcgtgcgc tttgccggtc agaatgccaa
     4621 aatcaaaacc cgcctgccga tagacggtca gaaaaacctt atcggtaaaa tcgaaggctg
     4681 cgaaaacgat accgttaccg tatccttcga cggcaaaacc gtacaaatcg aattgggcaa
     4741 catcgacaaa gcccgtctgc gccccgaatt caaattctaa aacacaacaa tattggagat
     4801 gttcaaaatg agtcgtgaaa tgttacagct ggcagaagca ctggcaagcg aaaaaaacgt
     4861 tgatgcggaa gtcgtcttcc aagcactgga attcgccctg tctaccgccg ccaagaaaaa
     4921 ggcagaccgc gaacacatgg acgtgcgcgt ccaaatcaac cgcgacaccg gcgaatacca
     4981 aaccttccgc cgctggctga ttgtcgccga tgaagactat acctatcccg atgtcgaaaa
     5041 aaccatcgag gaaatccaag aggaaattcc cggcactacc atccaaatcg gcgaatacta
     5101 cgaagagcag ctgcccaacg aaggcttcgg ccgccaagcc gcgcaaaccg ccaaacaaat
     5161 catcctgcaa cgcatccgcg atgccgagcg cgagcagaat ctgaacgagt ttctcgccgt
     5221 caaagaagac atcgtgtccg gcacggtcaa acgcgtcgaa cgccacggca tcatcgtcga
     5281 agtcgttgcc ggcaaactgg acgcgctgat tccgcgcgac caaatgattc cgcgcgaaaa
     5341 cttccgcagc ggcgaccgca tccgcgccct cttcctgcgc gtcgaagaaa tcggcaacac
     5401 cggccgcaaa caagtcatcc tgagccgtac ttccggcgat ttcctcgtca aactgtacgc
     5461 caatgaagta cctgaaattg cagacggcat gcttgaaatc cgtgctgtcg cccgcgaccc
     5521 gggccagcgt gccaaagtcg ccgtcaaagc caacgaccag cgcatcgatc cgcaaggcac
     5581 ctgtatcggc gttcgcggtt cgcgtgtcaa tgccgtcagc aatgaattgt ccggcgagcg
     5641 catcgacgtt gtcctttggt cgcccgagcc tgcccaattc gtcatgagcg cgctctcacc
     5701 cgccgaagtc agccgcatcg tcatcgacga agacaaacac gccgtcgatg tcatcgttgc
     5761 cgaagaccag ctcgcgctcg ccatcggacg cggcggtcaa aacgtgcgcc ttgcttccga
     5821 cctgaccggc tggcagctca acatcatgac ctctgcagaa gcagacgagc gcaatgcggc
     5881 agaagatgcc gccatccgcc gcctgtttat ggattacttg aacgtggacg aagaaaccgc
     5941 cgacgtactg gttcaggaag gttttgcaac cttggaagaa gtcgcctatg ttcctgccgc
     6001 cgaactgctt gccattgaag gatttgacga agaaatcgtc gatatgctcc gcaaccgcgc
     6061 ccgcgatgcc atcctgacca tggcgattgc cgccgaagaa aaactgggcg aagtgtccga
     6121 cgatatgcgc aacctcgaag gcatagatgc cgatatgctc cgcagccttg ccgaagcagg
     6181 cattaccacc cgcgacgact tggcagagct tgctgtggac gaactgattg aaatcaccgg
     6241 tgtaaacgaa gaaaccgcaa aagccgtcat cctgaccgca cgcgaacact ggtttaccga
     6301 agacaaataa agggggtaca gatgagtaac acaaccgtag aacaatttgc cgccgagctg
     6361 aaacgccccg tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag caaaaacagc
     6421 ggcagcgatt ccctgacgct ggacgacaaa cagcttctga acgcctacct gaccaagaaa
     6481 aacggcagca acggcggcac catcagcatc cgccgcacca aaaccgaagt cagcaccgtt
     6541 gacggcgtaa aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc ttctgccgaa
     6601 gaattggcag cacaggtaaa agccgcccaa acccaagccg cacctgtccg gccggagcag
     6661 acggcagaag acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga agcccgtgcc
     6721 aaggcagaag cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa agccaaacct
     6781 gccgcgcaga aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc ggaaaccaaa
     6841 cccgccgaag aaagcaaagc ggaaaaagcc caagccgaca aaatgccgtc taaaaaaccc
     6901 gtcgagccca aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa aggcaaagat
     6961 gccaaaaaac cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa
     7021 gaacaggcgc aacgcgatga agaagcacgc cgtgccgccg cacttcgcgc ccaccaggaa
     7081 gccctgttga aagagaaaca ggaacgccag gcacgccgcg aagccatgaa acaacaggca
     7141 gaacaacagg caaaagccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct
     7201 gccgagaaac cgcaggcagc cgcgccggcc gtcgaaaaca aacctgtcaa tccggcaaaa
     7261 gcgaaaaaag aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc
     7321 aaaggcggaa aaggcgggcg cgaccgcaac aatgcacgca acggcgacga cgagcgcgta
     7381 cgcggcagca aaaaaggcaa aaaactcaaa ctcgagccga atcaacacgc cttccaagca
     7441 ccgaccgaac ccgtcgttca tgaagttttg gttcccgaaa ccattaccgt tgccgatttg
     7501 gcgcacaaaa tggcggtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg
     7561 atggtaacca tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc
     7621 ggacacatcg gcaaacccgc cgccgccgac gatcccgaag ccttcttgga cgagggcgcg
     7681 gaagcggtgg aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggacacgtc
     7741 gaccacggca aaacctcgct gctggactac atccgccgtg ccaaagtggt acagggcgaa
     7801 gcgggcggca ttacccaaca catcggtgca taccacgttg aaaccccacg cggcgtgatt
     7861 accttcttgg atactccggg tcacgaagcg tttaccgcca tgcgcgcgcg cggcgcgaaa
     7921 gcgaccgaca tcgtgattct cgtggttgcc gcagacgacg gtgtaatgcc gcaaaccatc
     7981 gaagccattg cacacgccaa agcggcaggc gttccgattg tggttgccgt caacaaaatc
     8041 gataaagaca ctgccaaccc cgaacgcatc cgccaggaac tgacccaaca cgaagtgatc
     8101 cccgatgatt ggggcggcac ggttcaattc atcgacgttt ctgcgaaaaa aggaacaaac
     8161 atcgacgcgc tgctcgaagc agtcttgctc gaagccgaag tattggaact gaccgcacct
     8221 gtcgatgcgc ccgccaaagg catcatcgtc gaggcgcgct tggacaaagg ccgcggcgcg
     8281 gttgccacat tgctggttca aagcggtacg ctgaaaaaag gcgacatgct gctggccggt
     8341 acggcattcg gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc
     8401 ggcccgtcca tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac
     8461 gcgatggtat tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa
     8521 taccgcgacg tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat
     8581 atgggcgaaa cccaggccca atctttgtcg gtcatcatca aggcagacgt gcagggctct
     8641 tacgaggctt tggcgggcag cctgaaaaaa ctgtccacag acgaagtgaa agtgaacgtg
     8701 ttgcacagcg gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc
     8761 gcattcatta tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat
     8821 gaaaacgtgg aaatccgcta ctacaacatc atctatgatg ccatcgacga cgtgaaagcg
     8881 gcgatgagcg gtatgctttc tcccgaagag aaagaacagg ttaccggtac ggtcgaaatc
     8941 cgtcaggtta tctccgtttc caaagtcggc aatattgcag gctgtatggt taccgacggc
     9001 gtggtcaaac gcgattccca tgtccgcctc atccgcaaca acgtggttat ccacacgggc
     9061 gaactggctt cgttgaaacg ctataaagac gatgtaaaag aagtccgcat gggcttcgag
     9121 tgcggcctga tgattaaagg ctacaacgaa attatggaag gcgaccaact cgaatgtttc
     9181 gacatcgtcg aagtggcacg caccctgtga ttttgatgcg gaaaaatgcc gtctgaagcg
     9241 ttcagacggc atacgaaacg ggttctgtat catacagaac ccgttttttg tcgcaaatcg
     9301 gcttcagaca gccctcttgc cttatcccga tttgaatctg acttgccata caaacaggct
     9361 tcagacggca ttatagtgaa ttaaatttaa accggtacag cgttggctcg ccttgccgta
     9421 ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt aaatttaatt cactatattt
     9481 tcccgacagg cggatttgaa gtttctccac atatccggcg cgttcggcgc ggctggtttc
     9541 ggggcggtgc gtattgagcg acgaccattt ccaatgactg cgggctttgt tgagttcggg
     9601 cgggagtctg gcggcgtccc acggggcttt gcggctgtgc agctcgatat ccgactgtgc
     9661 cgcgtgtccg cgcgtttgca ggacgtggag caaatcgagg gcgcgggcgg cgagcagggt
     9721 caaggtttca gggtcggtgt gcagggtttg gcggccagcg agtttgtcgg aaatggtgcg
     9781 ggtattgggc aggatgccgc ccaaaaagcc gccgattgcc gtacccaatc cgagcgagcc
     9841 gccgagtgtg gcgatgtcca gccccaagcc gatgagcgcg ccagttgccg cgcccgtgcc
     9901 ggtgcggatg ccgtattgtt tgagcaattc gctgtcgaac gggtcttggc ggaaggcttg
     9961 cggcatccag tcgccgccgt cgatttcgct gtggtagaaa cggtaaaggg caaacagccg
    10021 ctgctgcatc tgccgttcga gttggcgtat ttccgcctgc atggtttgca gcacggtggc
    10081 ggtatcctcg ttttcgtcca cttcctgcct gaaggcggcg gcatcaatta aaaagtcggc
    10141 gatttcgcgg cgcgcttcgc cgtccagccg ctgccattcg cgccggcgca tggctgtcag
    10201 gcggtcaagt gtgctgcgtt cgggcaacat ggtggcgagg ttttcccaca ggcgtagttc
    10261 gccttcaaaa tcaaaggcga cggtgtcgaa ccctgcgaaa acgtgcaggt ttctcctcgc
    10321 cagcatggtt gtccacgatt cgggaggctg tccgccggta aagttgaaca cgggcataac
    10381 cggtttggca caccatgaaa ggatggtcaa ctcgtccctg tatttgtcga ggacgggttc
    10441 gcgcgcgtcg atgacgtaca ttgccatatc gctttgcaag acttgccgta agactttggc
    10501 ttcctgattg aaatcatggt gcgcgccgtg gctgccgaga aactgttgca gccgttcgat
    10561 gccgtctgaa cgattgtccg tatggttttc cagccattcc agcacgccgc ccgcgtcttc
    10621 gagtccgggc gtgtcgtaca ggaaaaccag cgtgtctgcg ccgtcgctga tggcggcttc
    10681 ttcgacatga cgcgtggtcg atggggcgtt tctgacttcg ccgaaaccgc tgtcgcgcaa
    10741 aagggtacga aggagcgagg ttttgccggt attggtatgc ccgacgacgg cgagggaaag
    10801 gggttgtttg ttcatgatgt ttttgaagaa tggattttca gacggtcttt tttcagaatg
    10861 gcggcttaac agaacatttc aagtgagttt attggtcttt caaacggcct tcctgcgcca
    10921 ccctgtcagg ctcaagccac gccgcgccgc attcggtcag cgcgttacgc caatgttcca
    10981 gcttttccgc aaggtcgtct gaaagcccct gttccgccag aagctgcacc accgcgccgc
    11041 cctgcgccgc ttccgagagc cggacaatct gccgcaacac gccccggtcc ggcacagttt
    11101 gggcgcgcac gccgataagc agttgcgccg gtttctgctt cagctctgtc tccagcgcgg
    11161 caacctgttc ccgattggcg gcaacgccct tatccagcca ttcctgcgcc agcctgcccg
    11221 taaaccatgt cccttcgtgc cactcggtct ccagcatgac cgcccatttc ggcgcatcgt
    11281 tcaagacgat tttcggcgaa acggcggaca cggtttcccg acgcgtatcc gcatcggtaa
    11341 ttttgttctg ccagcggcgg atgaccgcct gataataggg cttttccaaa tccaatccgt
    11401 tttcgcttgt tttcaaaagg attttgcata ccgcccaagc caagaggcgc ggcaggatgc
    11461 cgtagcaggc gatactgccg accagcagcc ccgaccaagc ccgcgcatcg gcaatattgc
    11521 cgttcagacg gccttcgatg accgcccgcg catcaggcac gggaaaaccc agtttcgcag
    11581 gcagccatgc caacatttcc accgcgcgta ccgaagcggc attgctcaac agcgtgcttt
    11641 cccagttgaa cgtatattgc cgcaccaaaa gcagcagcaa taccgacacc agcattccga
    11701 gcagcgtgca gagccacagg ctgtgcgacg ttgcgcctat tttccaacgt accgaaggtt
    11761 gccgccactc gtccgcatac agccgcaaca ccgcctgatt gacagggtct ttgccccgaa
    11821 accacgtcgc cggactgctg aaaaaacgcc ccactttcac gcgcaggaac aacgttgcca
    11881 accatactgc taacatcagc gtattcatgc ccaacacgcc cgccaaaatc aggaagaaat
    11941 tcagcccctg attgtccatc agaagataag tgcctgaaaa tccggcggta aacatcatcg
    12001 atgctgccac cacccacaac cagaacgacc ccacacgcac acgttccaac gtgtcccgca
    12061 gcatacggtt cctgtcgatc atctccgccc gacggatgat tttttcctcc gtactgccgt
    12121 ccacgcggcg caaagcctcc gtcgcctgca cgggatcgcc gctgaaaata tagccgccct
    12181 cttccaaaat gcgaaccaat tcaaccagtt tttgggaagg attcaacata aaatgccatc
    12241 tgaaaataaa aaaacagatt ttaacacacg cattttcaag aatattcaca gtgtaggcaa
    12301 agagtaaatc ccacacagag gcaaaagtat cggcgtaaac tgactgcctc tactttcccg
    12361 aaagattgtg cgatgtatac aggcgaacgc ttcaatactt acagccattt gagcggtttg
    12421 attctggcgg cggcaggttt ggcgctgatg ctgctgaaaa ccataggaca cggggacggc
    12481 taccgtatct tcagcgtatc gatttacggc atcagccttc ttctgctcta tttgagttcc
    12541 tcgctgtacc acggaattgc agccggaaaa ctgaaaagca ttttgaaaaa aaccgaccac
    12601 tgcatgattt atgtgctgat tgccggaagc tacacaccgt ttgcactggt ttctttgaga
    12661 aacgggccgg gctggacggt attttcactg tcctggctgc tggcggctgc aggaatcgca
    12721 caagaactca ccatcggacg gaaaagcgaa aaacgtctgc tgtctattgt gatttatgtc
    12781 gtcatgggtt ggatggtctt ggcggtaatg aaatccctga cagcctcact cccaccggca
    12841 ggactggctt ggctggcggc aggcggtatg ctgtacagtg tcggcattta ctggtttgta
    12901 aacgatgaaa aaatccgaca cgggcacgga atctggcatc tgttcgtatt gggcggcagc
    12961 atcacccaat ttgtcagcgt gtacggttat gtaatctgaa tgccgtctga aaagcaaaac
    13021 ctcccgttcc tgaagattgg gaggttttct ctttgccgga catcagccct tgtcgtggaa
    13081 ctcgtagaat tcatactgat aggacaaatc ctgtctcgcc tttttctgca tcaggtagtc
    13141 atcataaatg atgcggattt ccttacgcag caagaataag gcaatcaggt tggggataac
    13201 cataaagccg ttgaacatat cggacaggct ccaaaccaaa tcgactttgc cgagcgtacc
    13261 caaaacaatg gcaagcagaa ccaatgcgcg atagatgccc aagtgtcttc ccctgaaaag
    13321 aaaacggatg ttggactcgc cgaaataata ccaaccgata atggtcgtaa aggcgaagaa
    13381 ggtcagacac acggcaagca attgcgaacc gaagcccgga aatgccttgt taaaggcaaa
    13441 ttgagtaacc gccgcgccct gttcgcccga aaggttggca tcggtcagca ggataatcaa
    13501 tgcagttgcc gtacatacca aaatcgtatc gataaacaca ccgacaaatg ccgccatacc
    13561 ttgctgcaca ggatgcttca catccgcagt cgcgtgggcg tgcggagtcg aacccatacc
    13621 tgcttcgttg gaaaacagac cgcgcgccac gccgaaacgt atcgcttcgc gcataccgat
    13681 acccgcagca ccgcccaaaa cggcttcggg attgaaggcg gcggtaaaga tgtggttgaa
    13741 catcggcaca atatggtcgg aaaattcaaa caggataacg acggcgcaca aaatataaac
    13801 aaccgccata aacggcacga caaactgggc aatattggca atacggttca cgccgccaat
    13861 cacaaccatg cccgcaagga cggcaagcac aataccgact gccaaagaag gcacatcaaa
    13921 tgcaatggta acggcagaag cgatggagtt tgcctgagtc gcattgccga taaagcccaa
    13981 cgcgacaatc agcgcgatgg agaaaaagcc cgacaggaaa cgcgccgcgc ccctgccgat
    14041 tttcggagtc agaccgtgcg taatataaaa cgccggtccg ccgatgtatt tgccgtggct
    14101 gaccacgcgg tatttctgcg ccagcagtgc ctccgcaaaa accgtggaca tccccaaaac
    14161 ggcagaaagc cacatccaaa aaatcgcgcc cggcccgcct gcggtgatgg cggtcgccac
    14221 gccggcgaca ttgcccgtac cgatttgcgc ggatatggca actgccaacg cctgaaactg
    14281 cgataaagac ttgtcgtctt tatcgccttt ggcaaacaag ccgccgaata cggatttgaa
    14341 tcccgcgccc agcttggtaa tctgcggcgc accgagatac agcgtaaaaa acaggccgat
    14401 acccaaaagc gcgtaaatca gcaggtagtc ccaaaggaac cgattgactg tacccaccag
    14461 aacagacaat atattttcca taaaataaac cttatcttac gattaaaatg actgccttcc
    14521 aaaagacatt ccaataagga aacacggcga gcagaccgta tttgccacaa cagatgcctt
    14581 aaattgtcaa caatcgggga gaagctgcgc catcataccg taaaatatcg ttaattaaaa
    14641 catttcttta tttttaagcg gaaagcggag gaaatcgctt tcagacggca tcgacaacgg
    14701 cacggcataa aacaggatat tttgggtact tgcaacttat gttaaaatgc cgaccgtaaa
    14761 aaatctgaca aaaacagatt aattatttga aataagaaag gaaatttatg gcaggccata
    14821 gcaagtgggc aaatatccag cataaaaaag cccgtcagga tgccaaacgc ggcaaaatct
    14881 tcacccgttt aatcaaagaa atcaccgttg cggcgcgtat gggcggcggc gatcccggtg
    14941 ccaacccgcg cctgcgcctg gctttggaaa aagcagccga aaacaatatg cccaaagaca
    15001 atgtgcaacg cgccatcgac aaaggtacgg gcaacttgga aggcgtggaa tacatcgagt
    15061 tgcgctacga aggctacggc atcggcggcg cggctttgat ggtggactgc ctgaccgaca
    15121 acaaaatccg caccgttgcg gacgtacgcc acgcgtttac caaaaacggc ggcaacttgg
    15181 gtaccgacgg ctgcgtggcg ttcaacttcg tgcatcaggg ctatttggta tttgaacccg
    15241 gcgttgacga agacgcgctg atggaagcgg ctttggaagc cggtgcggaa gacgtggttg
    15301 ccaacgacga cggttccatc gaagtcatta ccgcgccaaa cgattgggcg ggcgtaaaat
    15361 ccgctttgga ggcggcaggt tacaaatccg ttgacggcga cgttacgatg cgcgcccaaa
    15421 acgaaaccga actctctggc gacgatgccg tcaaaatgca aaaactgatt gacgcgctgg
    15481 aagacttgga cgacgtgcaa gacgtttaca cttccgccgt attgaatttg gactgatacg
    15541 cagcacagca gacatacaat aaaatgccgt ctgaaccttt cagacggcat tgatttattt
    15601 agcccttgcc cacgcccacc aaaccgtcag gacaaccgcc agaaaatacg ccgcgctcca
    15661 aacaggcaaa gcaattccca gcagataatc cggttcggca caatttccga acccgcgcac
    15721 gacaggctcg aaccaatcaa acaaaggcca gcctttcaag cggaacgtcc acggcgcgcc
    15781 gcatggagga gccgtacccg gcggcagcga ctgcagccac aactgatatg ccgcaacaga
    15841 aatacccgta acggccggaa tgctgataaa cactgcgcct gacagtccgc ccaccctgcc
    15901 cttgggctta cacgccagca caattaccgc acataatgcg gttgccaaga cgcacaaccg
    15961 ctgactgata cacaaaacgc aaggctcaat acccaaaaca tactgtgccg ccaaagagcc
    16021 ggcaaatgca cagaccgaaa cggcaaacag cagccaaacg gcttttctaa ataacggggt
    16081 cattttctca acacaccaat caaaataccg atatgccgat tttgctggat atatcccgag
    16141 atggcaaggg acaaaacggc gatttgcccg cacaatgccc acaaaaaata catccggagg
    16201 atttgaattt cagcaaattt agcgaatcaa aagtttattt caatgaaatc atatgatttt
    16261 tttgaataag cggattgatg ggtttttgaa ggaatttgtt accggatagc catcgggcaa
    16321 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcggataa
    16381 gaaacggcgg ataattcccg ccgtcgagtt gcttgatgca gcttgatttt tctcctctat
    16441 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcattttttt atctccgctt
    16501 tttttgaaat ttaattactt ttattgtttg aaattccatt ctttaagaaa tttaacaaga
    16561 aaaagcttgc tttttatgac tgatagcggc ataatttgac ccgttagctg gttcgagtag
    16621 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctatcgaatg
    16681 tgtgcatttt tttatctaaa acgacaacat gccatacgtt ttgttgttat atttcattca
    16741 ggtgtcaaac tgcatagcca gcctgaaata ttcaatccaa atccaaaacc ggattttctt
    16801 tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt tgcaatagga
    16861 tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta atcagataat
    16921 cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc acttcttttt
    16981 tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca aaaaccaata
    17041 aaggtttgcc caacagatgg ggattgagat gggcgtgata gccggaaata taatgctccc
    17101 gctccaaacg gcgtacccgt tcggtaactg gagtagtgga caatcctact ttctcggcaa
    17161 gctccgtcat cggaatacgg gcatttttct gcaggatctt aaggatacgg aaatcaatct
    17221 tatctaactc tttcatttgt ctttacctta ttgtttatta ttgattttaa tggttgggat
    17281 gctgataagg aaagacaatt atatcaggaa aacgcaagcc tcctttccct atggcaaacc
    17341 caaccccacc tccgaagagt aaggttcttt aatattttaa aagccttgcc gttggattat
    17401 aatcccccaa ccgatttctt aattttgcta ataaacactt gtttggtaag gaatgaattt
    17461 atgcgtcctt tgaacgtgca gatcaggttg ggcaacctta ggcacaatta tcggattttg
    17521 aaggaaatgc acggaggcaa gctgttggcg gtagtgaagg ccgacgcata cggacacggt
    17581 gcggtcagat gtgctttcgc gctggcagac ttggcagatg gtttcgccgt ggccacaatc
    17641 gatgagggaa tcaagctgcg ggagagcggc attacccatc cgattgtcct tttggaaggc
    17701 gtatttgaag catcagagta cgaagcggtc gaacaatact cgctctggtc ggcagtcgga
    17761 aaccaatggc aacttgaggc tttgctttcc cgtcattgga aaaaacctgt caaggtctgg
    17821 ttgaaaatgg attcgggaat gcaccgtacc ggttttttcc ctcatgatta cgcttcggca
    17881 tatgcggcat tgaagcagtc ggaatatgtg gacagtattg tcaaattctc gcatttctcc
    17941 tgtgcggacg aacccgaaag cggtataacg gaaatacaga tggaagcatt cgatttgggt
    18001 actgaagggc tggaaggcga agaaagcctt gccaactccg ccgctatttt gaatgttccc
    18061 gaagcacgca gggactgggg gcgagccggc ttggcgttgt acggcatttc cccgttcgga
    18121 ggcagcgatg acaggctgaa acccgtgatg aggctttcca cccgtatttt cggcgaacgc
    18181 gttttacagc cgcactcccc tatcggttat ggcgcaacat tttataccag taaatctacg
    18241 cgcgtcggcc tgattgcctg cggttatgcg gacggttatc cgcgccgcgc cccaagcaat
    18301 tcccccgtcg ccgtcgacgg taaattgacc cgggtcatcg gcagggtctc tatggatatg
    18361 atgaccatcg agctggacgc ttcgcaagaa ggtttgggac acgaggtcga actgtggggc
    18421 gatacggtta atatcaatac cgttgccgaa gcggccggaa ccatccctta cgaattgatg
    18481 tgcaatatca aacgtgcaaa attcacttat atcgagtaat caagtccaaa caaaaatgcc
    18541 gtctgaagcc tttcagacgg cataaatgcg ttttgacaaa atcagtatag tggattaaca
    18601 aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa ggcgaggcaa
    18661 cgctgtactg gtttaaattt aggggctgtc ctggataact agga
//
LOCUS       DAWFIT010000037        17071 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFIT010000037 DAWFIT010000000
VERSION     DAWFIT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.037"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(27..653)
                     /locus_tag="WGQ85_001146"
     CDS             complement(27..653)
                     /locus_tag="WGQ85_001146"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0750578.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGQ85_001147"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGQ85_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0750579.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGQ85_001148"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGQ85_001148"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0750580.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WGQ85_001149"
     CDS             complement(3056..3553)
                     /locus_tag="WGQ85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0750581.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGQ85_001150"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGQ85_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0750582.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGQ85_001151"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGQ85_001151"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0750583.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WGQ85_001152"
     CDS             complement(5863..6309)
                     /locus_tag="WGQ85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0750584.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WGQ85_001153"
     CDS             complement(6314..6673)
                     /locus_tag="WGQ85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750585.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WGQ85_001154"
     CDS             complement(6746..7474)
                     /locus_tag="WGQ85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750586.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGQ85_001155"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGQ85_001155"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0750587.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8623..9333
                     /locus_tag="WGQ85_001156"
     CDS             8623..9333
                     /locus_tag="WGQ85_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0750588.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVLFPFAGRDLTLTRLPSTSRTYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            9416..10618
                     /gene="trpB"
                     /locus_tag="WGQ85_001157"
     CDS             9416..10618
                     /gene="trpB"
                     /locus_tag="WGQ85_001157"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0750589.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWAEFRCDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGQ85_001158"
     CDS             complement(10797..16271)
                     /gene="iga"
                     /locus_tag="WGQ85_001158"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0750590.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAKLAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAAAQPQAQAAAQPQAQAAAQADAVSTNTNSALSDAMASTQ
                     SILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGID
                     RSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLR
                     SRLQTQQKANFNRTSIQTGFTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKV
                     SSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQ
                     YSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         3468 a         4187 c         4646 g         4770 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcgactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 accgcttccg ctccggtgat gcggacggct tcgccgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccagcagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg gtggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtcgaact ggcggcagaa tgtgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggcacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg atttttttgg cgcgggcaat atcgacggcc
      541 cggctgctgc cttggaaaaa gaccagcccg acggcatctg cacctgccgc tacggcggcc
      601 gatgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacgat gcgtttgcac cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgttcaaaac
      781 cacttcgtcg ccttcgcgcg ctttaatcag gcaacgcgcc aacggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacaggccga cggtcgcgcc gaaaaatact tggtcggtcg cttcgcgcaa
      961 ttcgggatcg acgacgacgg cggactccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag atagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttgttga ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcgga ttgagtgctt tgacgacggc
     1501 ttccaaatcg ctcaaatttt ggaacacgat gccgtctgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgctcgttgg caatcaactc ttcgcgcgtg ggcatatcaa tgccgtacac
     1621 attgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgcccgccgc
     1681 gcgtaccatt tcgacgattt cgcggctggt cgtcccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctgtccgg gcataataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 accctcgcgg tagggtttgt cgagatggac ggcaagctcc atcgcgctgg ggcggctggt
     1921 gtcggggatg ggcatcacaa cgtcgatgcc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccagcgac acgcccatat ccaagcgcga ttggtaaacg gatacgccgt caatcacaga
     2041 gtcggggcgg gcaaaataaa catattcaaa aaggcagggg ctgagtttgg cacggtcgct
     2101 gcattggcgg gcaatcattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 gtcgcgttcc aaatcgtagg taagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggattttctg ccttcgctgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacgccgt atgcgccgcg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactt cgcggcgcaa ttcgtgcgcg aatacgttga gcaagacttc
     2461 ggaatcggag ctggtgttga cgtggcgtag gtgtttgttg cacacgtttt cataaagttc
     2521 ggcagtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaacggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgcccgc gttgcccgca gtaggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgtgtg cggaacactt cgcgcaccat
     2701 gcctttgcct ttgtgcatat ggaaggtacc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagagaat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccgg agtttccgcc gtgccgccgc tatggttcaa cacggcttcg gaaagcgata
     3121 cgaaaaacgg cagtgtgtaa gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca aacattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgccgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaagtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ccgtctgaaa
     6301 ggcgcgcaca gccttacatc atgttgtcga acacgggggt aatgttcaat tccgcttctt
     6361 ccatgtccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttatcga tgtcccaacc tgccagctcc gcctcgaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggctcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggatat gctgtctgaa
     6721 cgttcggttt tcagacggca tagcatcaat gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat cgtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgttgct gctggccgcc ggaaagttgg gaagggaagg
     7081 cgtctttttt gtgtgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctctgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt aatgttttcc aacacggtca
     7201 ggtgcgggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcttca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gctacgtatt tttccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tactcgaaat cgtccgccct gccgatgcgg
     7861 tgtttcaccg gaatcatgcg gacgacggcg gaatcgactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgataatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttccttttc ggttgaaacc ccgcccttta
     8461 gggcggtaga atcagactct atttgggagg ggcgtaaccc cttccaaatc aggatggcac
     8521 atagggcggt gctttatgtg tcgtcctgtg tgttgaaaca taaatgtgtt tacagtatcc
     8581 gtttgatgtc ggcattgtaa ccgaaaacgg cagggcgtga taatgctgtt tgaaggcttg
     8641 ccgtgtttgg cggtttggtg caaaaaccgg ctgtctgccg ttttgcctgt tggaggattg
     8701 aacgtgtctg aaaatctgct tgaaatcgaa acccatccct tcgattccgt gttgccgccg
     8761 aaggctgcgg tcatgatgat ggggacgttt ccgcccaagg aagacaaacg cgcgatgcag
     8821 tttcattatc cgaatttcca aaacgatatg tggcgcgttt acgggctggt gtttttcggc
     8881 gatgcggatt atttcaaagt gccgtccaac ggggaggctg cgcactctga aaaagcgttt
     8941 gatgccgaga aaatcaaggc gtttttgcac gaacggggga ttgcgtcctg tccgaccgtt
     9001 ttgaaggcgg tacgtcagca cggcaatgcg tccgacaagt ttttaaaggt agttgaaacc
     9061 gtcgatttgg cggcggtgtt ggcaaaaata cccgagtgcc gccatatttg tacgacaggc
     9121 ggcaaggcga cggaaatcct gctcgatatt cagggtggcg gcatcaaaat gccgaaaacg
     9181 ggcgaaaccg tgctgtttcc gtttgccgga cgggatttga cgctgacgcg cctgccttcg
     9241 acttcgcgca cctatccttt gagtttggcg aaaaaagcgg cggcgtatcg ggcgtttttc
     9301 gaaatggcgg gcttgtgtga aaagcagtta taattgccga caatttcccg ttcggacggc
     9361 atgtttgaaa aaacggaaat gccgtttgaa aacctgaagc acaaggaaga atccgatgaa
     9421 gaactaccac gcgcccgacg agaagggctt tttcggcgaa cacggcgggc tttatgtttc
     9481 cgagaccctg attcccgcct tgcaagagct ggcggatgcc tataaggcag cgaaaaacga
     9541 tcctgaattt tgggcggagt ttcgctgcga tttgaaacat tatgtcggca ggcccagccc
     9601 cgtttaccac gccgcgcggt tgtccgagca tttgggcggc gcgcaaatct ggttgaagcg
     9661 cgaagacttg aaccacaccg gcgcgcacaa agtcaacaac accatcggtc aggcactgtt
     9721 ggcaaaacgc atgggtaaaa aacgcgtcat cgccgaaacc ggcgcgggtc agcacggcgt
     9781 ggcgagtgcc accgttgccg cacgcttcgg tatgacttgc gacgtgtata tgggcgcgga
     9841 cgacatccaa cgccaaatgc ccaacgtgtt ccgtatgaaa ttattgggcg cgaacgtggt
     9901 cggtgtagaa agcggcagcc gcacgctgaa agacgcgatg aacgaagcca tgcgcgaatg
     9961 ggtcgcccgc gtggacgaca cgttctacat catcggtacc gccgccggcc ccgcgccgta
    10021 tcccgaaatg gtgcgcgatt tccaatgcgt gattggcaac gaagctaaag cgcagatgca
    10081 ggaagccatc ggcagacagc ccgacgttgc cgttgcctgc gtgggcggcg gatcgaacgc
    10141 catcggtttg ttccacccgt atatcggcga agaaaacgtg cgcctcgtcg gcgtggaggc
    10201 tggcggtttg ggcgtgaaca cccccgatca cgccgcgccg attacttcgg gcgcaccgat
    10261 tggcgtattg cacggtttcc gcagctatct gatgcaggac gaaaacggtc aggttttggg
    10321 tacgcactct gtttccgcag gcttggatta ccccggcatc ggcccggaac acagccatct
    10381 gcacgacatc aagcgcgtcg aatacactgt tgccaaagac gacgaagcac tcgaagcctt
    10441 tgacttgctc tgccgcttcg agggcatcat ccccgcgctc gaatccagcc acgccgttgc
    10501 ttgggcggtg aaaaacgcgc cgaaaatggg caaagaccaa gtgattctgg tcaacctatc
    10561 cggtcgtggc gacaaagaca tcaataccgt cgcgaagctc aaagggatta aactgtaacc
    10621 tcgtccgtct gatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttgccgta
    10681 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
    10741 atgccgtctg aagcctgagt tcagacggca ttttattttg ctatgaattt agtattttag
    10801 aaacgaatct gtattttaat ttgtccggat ttttgttttt ccaattgttt tccttttgta
    10861 atactgccat ttacgtttaa tgtaacatta cggtacagta acgcggcacc tgctgaatat
    10921 tgctgttgat tatctgcttt atagacgaag gaattaccgc ccacattcac gccgcctttg
    10981 ccataattgg caaagtaagc tgcagataac aagggtttta cggtaaggtt gccgacttta
    11041 aaccgataag caaaatccag tccggccgtt agtgttttca ctgccataga acttacttta
    11101 acactgtcgt tacccaactt gtaatctgca gatgacaggc ggctgtaacg gattcccgca
    11161 ctggggacaa tctcgaattg attgattttc agcgtattgc ccaaagtaaa gccggtttgg
    11221 atgcttgttc ggttaaagtt tgctttttgc tgcgtttgta accggcttct caagctgcct
    11281 gcaccaatat cgccggccac ataccaagca tcatttaaat aatacttacc ataaaggttg
    11341 gcttgcacaa aagtattttt gccgctcgcc tgatcaaaag tatgctgact gtcagagtaa
    11401 gtcaatacgc cgcctatctg catattttcg gacaagctgc ggtcaatgcc gatttgtgtt
    11461 tgcgtgcgtt tcgaactaaa ccggcgatat tgtgcggaag cataatcacg gccataaccg
    11521 atgttcgaca tccaaacgct gtttttttcg gcatcagcac gtgatttttg tgcaatgtgc
    11581 cgtgttaatg aagcacctgt atccaacaag atagattgcg tgcttgccat tgcgtcagat
    11641 aaagccgagt tggtattggt gctgactgca tcggcttgcg cggcggcttg ggcttgcggc
    11701 tgagcggcgg cttgggcttg cggctgagcg gcggcttggg ctcgcggctg agcggctctt
    11761 ggttgcaaac tcactgtctc aactttttca tgaagttccg tattgtcttg aggctgtttg
    11821 tctgatgtgt caaccgattc ggatacatct tcatcttcca acgcatccaa ggggatttct
    11881 tcataatcat tttcatattt ttcatgaagt tccgtattgt cttgaggctg tttgtctgat
    11941 gtgtcaactg attcagatac attttcatct tccaacgcat ccaaggggat ttcttcatag
    12001 tcattttcat accagtccgg attatgcaag gccctgggtg ctgcgtaagc tgacgaatca
    12061 aatgatggcg agggcggtgc cggcagagta gatctgcgtc cgcgacgttt cggacgattt
    12121 tgggaagctg ccatataatc ctgaggtgcg gcacggcgtt ttcggcgttg cggctgggat
    12181 tgggctgctt gacggtgctc ttcttcctca gcttttcgtt tcgccgattc tgccgcttct
    12241 cgatctgttt cggctttttg ttttgccgac aactctgctg cttggcgttc cgcttcttga
    12301 cgacgtgcaa gttcggcggt ctggcgagct tcttgctggc gttttgctgc ttcggctgct
    12361 gccctttgct tggcaagttc ggcggttttg cgttcggttt ccgctttttg tttgacagct
    12421 aactctgcag cttttcgtgc ttcttcctgt tgacgcgcaa gggctttttg ttggcgtgcc
    12481 gccaatgctt gggcttcagc tgcggctttt tggtttgccg acaattctgc cgctttgcgt
    12541 tctgcttcct ggcgatgtgc aagttcggca gcttggcgtt ttgcctcttc ggcttctgct
    12601 tttcggcgcg cagccaatgc ttgagcctca cgttcggctt ctgccctttg ttttgccaac
    12661 aactctgccg ctttgcgttc ttcctgctgt cggcgcacaa gttctgcttg ggcttgggca
    12721 atttccacat tgttttgctg taccgaacgg tgtccgcggc gtttaggacg gtcttgggaa
    12781 gctgccatat aatcctgcgg tgcggcacgg cgtttgcggc gttgcggctg ggattgggct
    12841 gtttgacggc gttcctcttc cccgaccctt tgttttgccg acaactctgc cgcttcgcgt
    12901 tctttttcat gccggcgtgc aagctctgca gcttggcgtt ttgcctcttc ggcttctgct
    12961 tttcggcgta ccgccaatgc ttgagcctca cgttcggctt cgactttttg ttttgccgac
    13021 aactctgccg cttcgcgttc tttttcatgt cgacgtgcaa gttcggcgct gctgcgttct
    13081 tgttctgctt tttggcgggt cgccagctct cttgcttcgc gttcggcttc tgctttttgt
    13141 tttgctaact ttgccgattt acgctctgct tctgcttgct gacgcttcac ttgctccgct
    13201 tttgcttgct ggcgttttgc ttcttcggct tgatttgcct gcgggctagg cggtgcgacg
    13261 acgatatttt gaggcttggc aatttgtgca ccgtccgttt gtgttgcctt ttgtgcttga
    13321 gaagccgtgt ttgtggcagg agacggggcc ggtttgactc ggcggcggtt ctcggcataa
    13381 ggattgtaca atcgggtaat gccattttct gttttgattg tataacgcaa tgcgcctaaa
    13441 tctacatggt tattcgccaa ggaaacagaa aggcgggagc ggtcttgtac ggatgatgca
    13501 tcaaagagat tcagcccttc ctgattgggt tcgcctgttt tatcttgaac atggagctga
    13561 taatgaccgg atgcggattc cttcacaagc actttatccc caagattttc cgccaagtgc
    13621 gtcagataat gaaaatgccc gttaccggat aaatgattga ttttgagcgt gtggtattga
    13681 tttgcacttt gcgcatcgga ggcattgttc aaatgaatat ggctatccgc caatgacaga
    13741 ttgtgtactt ggctgtcgcc agtcaaatgc catttgctat gttggtttag gctgacacgg
    13801 ctgtttcctt gcccttgaat ttgcccccat aatgcagcct tgcccaagac caatgccgca
    13861 ttctggttca gattcacatt gccgttaatc tgtgtcgctc caaagctgtt taaagcctta
    13921 tcggataagt tgcctgtgtt gcaggtaaca taaccggtat agtccgagcg cacgcaaacc
    13981 tcatcgccgt ttttgtaacc caaatttact ttggcgttgt ctgtcgcggt gatgttggcg
    14041 gtaatgttgg atacatttct accggaagag aatgatgcgg attgattaac cgcaatttct
    14101 gcggctttga atgtgcggtt tatccagtcg tcttcaaata cgacttcatt gtttttggag
    14161 aaatgtgcgt cttttcgggc tgaagatttg ttcacaaaat ctcttgcgtg tggtgttgga
    14221 cgacctgata acaagacatt gccttgagtt acgcttattt ttccgtttaa attagtgccg
    14281 cctgttaaca agaaacggtt ttgcgcgctt ttgccgttga aattaaggtt taatgcaccg
    14341 ttatgtcctt ttccgttttc ttcgccaaag aaaccgctaa aaccgctaat acgctgattg
    14401 tttttgtggt tcatcgcgtt tttcttggct tcttcttgcg tatatcccat aaagacccag
    14461 tcgttatttt ctgttacacc gttctcgggc atcggtgcgt tcacgctgcc gccggatttt
    14521 agggcgtaat aacgatagtt tttgtaataa aggtctttgc cttgcggaat cgggcgcctt
    14581 ggtcggtagt aataactata atcatcttcg tcatagtcat tttgtatgct atgtaccgaa
    14641 aggctgttcg gattggtaat taaagattta cccgttagcg tgattgtgga ggcgtggctt
    14701 gtgttgtggt tgacaatgcg cgcgccttca tccacgttgc ggatgtgttc aaaagtcaag
    14761 tcattgccat tggcatccaa acgaccgcca cggaaaccga aatataggtt atcgggatta
    14821 atctgatttg aactatttaa taccaatgta ccgcgtccgc tgacaatgcc gacttgggag
    14881 aaagcctgga cttttttgtc ggcatcggct tgttgattca gaataaccgt accgtcgccg
    14941 acttttaatt gcccttggtt aacgcctgtg ccgtttattt ctaatgtgcc tttgccgatt
    15001 tttgccaatc tgtcgccatt cggatttttg acttgccaaa cgactttttt gccgtcggta
    15061 acatcaatcc ccgcacctag ccaagtgatg tcattattta tacctttgac tgtgtaatcg
    15121 cctttgaaaa acagaccgcc cgcgccttgg ttgatgtttt gatccaatac caaagtgccg
    15181 ttgtcttcaa aggtaacatt ctgtccgttg tttgcatcct tttcattgcc ggcaagcctt
    15241 accgctgtag aaccaatctt gctgtttgtg ccaaaagtaa tgttccaatg atgttctcca
    15301 ttacctttga tggtgccggc gttatcgcgt tgtttgattt tatctgcaaa ttctttttta
    15361 tagatattcc attcttgcca agagtttttt tgatagcctg cccaataatc gtaagcacct
    15421 aaaaagaccc agcgtttttc ttgtttatca taagcaaata atggtgaacc gctatcgcct
    15481 aacacagcat agttagtcaa tgcgttttga gataaaactt ctttgagttt ttctggggaa
    15541 tgatgttttg aattatcacc gaagccaatc aagccttctt gatttagatt cgatgtgaca
    15601 ttcacgtctt gataaggcgt acctgcaatg gcataacgat aagctcgtga aagctctgtc
    15661 atattgtagc ggctgttata ttcaaattgc gtacctgctc caactcgcac aaactcagaa
    15721 aaacggtttt tatctttata ggtttcaacg ccgccgcctg cactggttgg tgcaataggt
    15781 gcgacttctg ttacgaattt attaaggcgt gccatgttgt agtcttcaag acgaccttga
    15841 ttaccgtgat gccaattttt atttggttcg tagtcatttt gtgcaactga acgatattcg
    15901 ttttcatcat tgctaacatc taagtgccca ttgtgatgcc cgtaataaga gatttcgtct
    15961 ccttttacat gtttgacgct gacggcatac tggggatcga tgacggttaa tgtacgtctg
    16021 ttgacatctg caacgctaaa atcaatcatc ggtacgttgg ataatgcgtt gccgatgttt
    16081 tgaccttgtt tgtttttcac tgataaatcg gttgcgccga caaaaaattt gcctttgttt
    16141 tctgcaaagt cacggaatat ttgataatcg acatcgtctc tgaccaatgc cgcttctgag
    16201 tatggcgtaa gggcataggc aagaaagatg gataaggata tggcgttaat tttaaaacgt
    16261 ttggttttca taaggtttta ccgttttaag ggtgataatg ttttgtattt tacgccggtt
    16321 tggcggtgtt gactattttt tgttgtgtga atcattaatt ttggtgtttt attgccaatt
    16381 taaaaaaaga atcccgatgt ttttatttcc gcttcctttg ttctgttatt caagcgaagg
    16441 cgggaagccg gttttcgggg ttcggttctt ccgttaaatt tctgcggctt tttgtttttg
    16501 gattcccgct tttgcgggaa tgacgggatt ttaggtttct gattttggtt ttctgttttt
    16561 gagggaatga cgggatgtag gttttcttaa ccctgcgtcc tagattcccg ctttcgtggt
    16621 aatgacggga tgtgggttcg tgggaatgac gcggtgcagg tttccgtacg gatggattcg
    16681 tcattcccgc gcaggcggga atccagacct tagaacaaca gcaatattca aagattatct
    16741 gaaagtccga gattctggat tcccactttc gtgggaatga cgaaaagtgg cgggaatgac
    16801 gaaaagtagc gggaatgacg aaaagtggtg ggaatgatgg ttcgggcatt ccttaaatcc
    16861 cccgtgtatc gctgtaaatc ttagagatgg cggaatatag cggattaaca aaaaccagta
    16921 cagcgttgcc tcgacttagc ttaaagagaa cgattctcta aggtgctgaa gcaccaagtg
    16981 aatcggttcc gtactgtttg tattgtctgc agcttcgccg ccttgtcctg atttttgtta
    17041 atccactata aagaccgtcg ggcatctgcg g
//
LOCUS       DAWFIT010000038        16589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFIT010000038 DAWFIT010000000
VERSION     DAWFIT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.038"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            209..1273
                     /locus_tag="WGQ85_001159"
     CDS             209..1273
                     /locus_tag="WGQ85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0750591.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WGQ85_001160"
     CDS             1414..1737
                     /locus_tag="WGQ85_001160"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0750592.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WGQ85_001161"
     CDS             1809..2132
                     /locus_tag="WGQ85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0750593.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGRQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WGQ85_001162"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WGQ85_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0750594.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WGQ85_001163"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WGQ85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0750595.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WGQ85_001164"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WGQ85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0750596.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WGQ85_001165"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WGQ85_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0750597.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WGQ85_001166"
     CDS             4530..4883
                     /locus_tag="WGQ85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0750598.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WGQ85_001167"
     CDS             4873..6687
                     /locus_tag="WGQ85_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0750599.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLSIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WGQ85_001168"
     CDS             6877..7554
                     /locus_tag="WGQ85_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0750600.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WGQ85_001169"
     CDS             7568..8974
                     /locus_tag="WGQ85_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0750601.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WGQ85_001170"
     CDS             9092..11518
                     /locus_tag="WGQ85_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0750602.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     HAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNDTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WGQ85_001171"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WGQ85_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0750603.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WGQ85_001172"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WGQ85_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0750604.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WGQ85_001173"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WGQ85_001173"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0750605.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WGQ85_001174"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WGQ85_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0750606.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13785..14510
                     /locus_tag="WGQ85_001175"
     CDS             13785..14510
                     /locus_tag="WGQ85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750607.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14534..15409
                     /locus_tag="WGQ85_001176"
     CDS             14534..15409
                     /locus_tag="WGQ85_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0750608.1"
                     /translation="MNLYDLLLAPFAEFDFMCYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15486..16400
                     /locus_tag="WGQ85_001177"
     CDS             15486..16400
                     /locus_tag="WGQ85_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0750609.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQNLVGANQDTHAYHMTSGDIKKIRSAKLVLINGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
BASE COUNT         4335 a         5288 c         3640 g         3326 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagcaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cagacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac gcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag
     2641 ggtaatctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctattagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtagag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacggcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctaccgcgtc atccaactgc ctgccggcat cgacgaatcc gttaaagccg
     4021 gcgaaacacc tttcgcttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt cccttgcttg ggaactgtgg attgcaccgt
     4621 tacgcccggg cggttcgtgg cttgcgctca aaaccctgcc cctctgcctg ccgctttcag
     4681 gcatcctgaa gaaaaaaatc tacacctacc aatacagctc catgttgatt ctgatttatt
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatccggc agaacaaatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgccc gtatccggtg cgcgccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aggcagcaac cgtcgcccga cttttaccac gatgctgcag ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cagcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc tttatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa taccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagctg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgcctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc acgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcaaccg tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag ccctcgaagc cgccgacccc gccctaatcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgtc caattccaat tcggcagcca
    10501 ctccctctat ggcgacatcc tgaccgcacg ttgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac cgtcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcacacccca aaacactgtc
    10981 cctaaaaaac gacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaatcgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcacacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccctttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa cgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatc cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacagcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcacggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt actcgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcttccc aagctgcaaa agtcatttac ctcagccccc
    12601 aaggaaaacc gctgacacac ccaaaagcgg cagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acgctatgag ggaatagacg aaagactgct gcaaaccagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcagtttaa
    13021 tcccaaagga atcccgcatc ttgaataaaa tcctacaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggtc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgcc ccgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatct acctttctta
    13921 aatccttgat gggattgcag cccatcgaca ctggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcaat ccgacatcga ccgctcgcag cctatgaccg
    14041 ttttcgactt ggcggcaatg gggctgtggt atgaaatcgg ttttttcaga ggaataaatg
    14101 ccgttcaaaa acaacgcgtt cacgaatctt tggaacgcgt cgggatgcgg cagtttgccg
    14161 gccgccaaat tgcccacctt tccaacggac aattccagcg tgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctgg acgaaccttt caacgctgtt gatgcacgga
    14281 caacctacga gcttctcgac gtgttgcaaa aatgccattg cggcggacac gctatcattg
    14341 ccgttttgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcgtcataa atgccgtctg
    14521 aaaccgaaaa accatgaacc tctacgacct gctccttgcc ccctttgcag aattcgactt
    14581 catgtgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac ccgtcggcgt
    14641 attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg ccgtcttgcc
    14701 cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg gtttgggcgg
    14761 cgtagccgca ggcatgctga tggcactgct tgccggactt gttagccgct tcaccaccct
    14821 gaaagaagat gccaactttg ccgcctttta tctcagcagc ctcgccatcg gtgtagtcct
    14881 cgtcagcaaa aacggcagca gcgtcgattt gctgcatctg cttttcggct ctgtacttgc
    14941 cgtcgatatt cctgccctgc aactcatcgc cgccgtatcc accctcacac tgcttaccct
    15001 tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgttcc tcaaatccgt
    15061 cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca tgaacctcgt
    15121 atccggcttt caagccctcg gaacactcat gtccgtcgga ctcatgatgc tgccagccat
    15181 taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg ttctgacagc
    15241 cctgctgtgc ggcttgagcg gactgcttat ttcctaccac atcgaaattc cctccggtcc
    15301 cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg gcaaagaagg
    15361 cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac cccgcaagcc
    15421 gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat cacaggagaa
    15481 cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc gccgcaactg
    15541 ccgcccccct tccggttgta accagtttca gcattttggg cgacgtagcc aaacaaatcg
    15601 gcggagagcg cgtatccata caaaatttag tcggcgccaa ccaagatacg cacgcctatc
    15661 atatgaccag cggcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg attaacggct
    15721 tgggacttga agctgccgat gtgcaacgcg ccgtcaaaca aagcaaagta tcctataccg
    15781 aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac catcacgacc
    15841 acgatcatga ccacgaagga caccaccacg accacggcga atatgacccc cacgtctgga
    15901 acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg ataaaggccg
    15961 accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg cagctcaaaa
    16021 aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa cgcaaagtcc
    16081 tgaccgggca cgatgccttt tcctatatgg gcaaacgtta ccatatcgaa ttcatcgccc
    16141 cgcaaggcgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc atcatccgac
    16201 aaatcaaacg cgaaggcatc aaagccgtat ttaccgaaaa tatcaaagac acccgcatgg
    16261 ttgaccgcat cgccaaagaa accggcgtca acgtcagcgg caaactgtat tccgacgcac
    16321 tcggcaacgc gcccgcagac acctacatcg gcatgtaccg ccacaacgtc aaagccttga
    16381 ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg caaaaggccg
    16441 tctgaaaatt atagtggatt aacaaaaatc aggacaaggc gacaaagccg cagacagtac
    16501 agatagtacg gtaaggcgag gcaacgctgt actggttttt gtgaatccac tatattgcta
    16561 gaagtgcacc gattaaggtt aattaaaat
//
LOCUS       DAWFIT010000039        16208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFIT010000039 DAWFIT010000000
VERSION     DAWFIT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.039"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..69
                     /locus_tag="WGQ85_001178"
     CDS             <1..69
                     /locus_tag="WGQ85_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0750610.1"
                     /translation="LRFAIREGGRTVGAGVVSSVIA"
     gene            76..151
                     /locus_tag="WGQ85_001179"
     tRNA            76..151
                     /locus_tag="WGQ85_001179"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Trp,seq:cca)
     gene            266..544
                     /gene="secE"
                     /locus_tag="WGQ85_001180"
     CDS             266..544
                     /gene="secE"
                     /locus_tag="WGQ85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0750611.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            546..1082
                     /gene="nusG"
                     /locus_tag="WGQ85_001181"
     CDS             546..1082
                     /gene="nusG"
                     /locus_tag="WGQ85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0750612.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1183..1617
                     /gene="rplK"
                     /locus_tag="WGQ85_001182"
     CDS             1183..1617
                     /gene="rplK"
                     /locus_tag="WGQ85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0750613.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1617..2312
                     /gene="rplA"
                     /locus_tag="WGQ85_001183"
     CDS             1617..2312
                     /gene="rplA"
                     /locus_tag="WGQ85_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0750614.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2542..3042
                     /gene="rplJ"
                     /locus_tag="WGQ85_001184"
     CDS             2542..3042
                     /gene="rplJ"
                     /locus_tag="WGQ85_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0750615.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3100..3471
                     /gene="rplL"
                     /locus_tag="WGQ85_001185"
     CDS             3100..3471
                     /gene="rplL"
                     /locus_tag="WGQ85_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0750616.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3662..7840
                     /gene="rpoB"
                     /locus_tag="WGQ85_001186"
     CDS             3662..7840
                     /gene="rpoB"
                     /locus_tag="WGQ85_001186"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0750617.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7992..12167
                     /gene="rpoC"
                     /locus_tag="WGQ85_001187"
     CDS             7992..12167
                     /gene="rpoC"
                     /locus_tag="WGQ85_001187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0750618.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12458..12715
                     /locus_tag="WGQ85_001188"
     CDS             12458..12715
                     /locus_tag="WGQ85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750619.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12712..12861
                     /locus_tag="WGQ85_001189"
     CDS             12712..12861
                     /locus_tag="WGQ85_001189"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0750620.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            13040..13411
                     /gene="rpsL"
                     /locus_tag="WGQ85_001190"
     CDS             13040..13411
                     /gene="rpsL"
                     /locus_tag="WGQ85_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0750621.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13529..13999
                     /gene="rpsG"
                     /locus_tag="WGQ85_001191"
     CDS             13529..13999
                     /gene="rpsG"
                     /locus_tag="WGQ85_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0750622.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14018..16123
                     /gene="fusA"
                     /locus_tag="WGQ85_001192"
     CDS             14018..16123
                     /gene="fusA"
                     /locus_tag="WGQ85_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0750623.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4298 a         3599 c         4204 g         4107 t
ORIGIN      
        1 ctgcgctttg cgattcgcga aggcggtcgt accgtgggtg ccggcgtggt ttcttctgtt
       61 atcgcttaag tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg
      121 gttgggggtt cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt
      181 taattttttt gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag
      241 acagatgcta tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa
      301 agtggatcaa ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt
      361 ttttgcatat ttctcaaatt cttggttcga attcaaaaag gtggtttggc ctaagcgtga
      421 agatgctgtc agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat
      481 ctatgcggca gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg
      541 ttgagatgtc gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc
      601 aacgaatatt ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc
      661 tggtgcctgt agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa
      721 agtcatatcc tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg
      781 taaaaagcac cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga
      841 ttagtcagag agaggctgaa attattttac agcaggttca gaccggcata gagaagccga
      901 aaccaaaagt tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg
      961 attttaacgg ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc
     1021 agatatttgg tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact
     1081 gatttttata cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc
     1141 ggaaatggtt ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc
     1201 ggctatatta aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct
     1261 gctttgggtc agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc
     1321 caaggtatgg agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca
     1381 ttcacatttg tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg
     1441 caaaaaggta gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg
     1501 gaagaaattg ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc
     1561 cgtactatag caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg
     1621 ctaaagtatc taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg
     1681 caattgatga agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg
     1741 ttgacgtatc tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt
     1801 cagtcgttct gcctaaaggc accggtaaga taactcgcgt agctgtattt actcaaggtg
     1861 caaatgcaga agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg
     1921 ctgaaatcaa agcaggcaat ctgaactttg atgtcgttat tgcttctccc gatgcaatgc
     1981 gtattgttgg tcagttgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag
     2041 taggtactgt tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtac
     2101 aataccgtac agataaagca ggtatcgttc atgcaacgat tggtcgtgct tctttcgctg
     2161 aagctgactt gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg
     2221 ctgccgctaa aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta
     2281 ttcgcgttga tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt
     2341 ttctgggctg cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat
     2401 cgtaagattt aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt
     2461 gcttgtaaga tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt
     2521 ataaacagtg ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag
     2581 gaaattagcg cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc
     2641 agtgtttcca gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc
     2701 gttctgaaaa atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc
     2761 gatcaaatgg ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa
     2821 gtgttgcacc aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat
     2881 ggcgaagtaa tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag
     2941 ctgttgtcca aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg
     3001 gctgctttgg cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta
     3061 atcaattatt ttttaataca atatttggag taaaatagca tggctattac taaagaagac
     3121 attttggaag cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt
     3181 gaagaaaaat tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt
     3241 gctgccgatg ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggtgatcaa
     3301 aaagtcggcg tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa
     3361 gacatcgttg acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa
     3421 gacatccaaa aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct
     3481 tcttatgaag gctggcagtt ttctgccagc cttattttgc ttcttaaaat aagcattaag
     3541 tattgtttac atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca
     3601 gattgatgcg tgccgttgct ttcagacggc ctattattga aaattacttt tcggagtgta
     3661 tatgagctat tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa
     3721 tgttttggaa gttcctttct tgctggcaac ccaaattgat tcttatgcga agtttttgca
     3781 gctggaaaat gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat
     3841 tttcccgatt gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg
     3901 cgagcctttg ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt
     3961 gcgcgcgcgt atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga
     4021 agttcgtgaa aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt
     4081 tgtgattaac ggcacagagc gtgtgattgt ttcccagttg caccgttcgc ccggcgtgtt
     4141 tttcgagcat gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat
     4201 cattccctac cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt
     4261 ccgtatcgac cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa
     4321 caatgagcaa atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg
     4381 tgttcaaacc gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt
     4441 ggataaagaa ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg
     4501 tgatattacc aatgcaggcc tgacccgttt ggatgtagaa ccagaaagcc tgctgggcaa
     4561 agcattggct gccgacctga ttgattcgga aaccggcgag gtattggctt ctgccaatga
     4621 tgaaattaca gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac
     4681 cctttacatc aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga
     4741 gactgccggc cggcaggcgg cgcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc
     4801 gcccactgaa gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta
     4861 cgatttgtcc cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc
     4921 cgaagcccaa caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc
     4981 cgacaaaggc ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt
     5041 cgagttgcgt aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt
     5101 acgttcggta ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg
     5161 tgccgtaaaa gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat
     5221 taatgcaaaa cctgtttctg ctgctattaa agaattcttc ggctccagcc aattgagtca
     5281 gtttatggat cagaccaatc ccttgtctga agtaacccat aaacgccgcg tatccgcatt
     5341 gggtccgggc ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac
     5401 ccactacggc cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa
     5461 ctcactgtcc gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg
     5521 cgttatcgac ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg
     5581 ctatgtgatt gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt
     5641 ggttacctgt cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat
     5701 ggacgtggca accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca
     5761 tgatgacgcg aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt
     5821 gcgtcctgaa aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc
     5881 tactgcaatc gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgcgtcgt
     5941 cgttcgtgtt catgacgatg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt
     6001 ggttaaattt acccgttcca atcaatctac taacatcaac caacgtccgg ctgtcaaagc
     6061 aggcgatgtt ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga
     6121 attggctttg ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga
     6181 agactcgatt ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat
     6241 tgaggaattg aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga
     6301 tattccgaac ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat
     6361 cggtgcggaa gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac
     6421 ccagctgacg ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt
     6481 aaaagatact tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt
     6541 cttcactcgt gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgagtt
     6601 gaaacgctac cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga
     6661 ccgtatcgag cgtatgattg tcggtcagaa agccaacggc ggcccgatga agctggccaa
     6721 aggcagtgaa atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga
     6781 tatccgtctg accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca
     6841 acaaaaacgc gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg
     6901 cgacgaattg caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg
     6961 tctgcaagcc ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat
     7021 tctgccagtg gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa
     7081 cccattgggc gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg
     7141 ggcagcaaaa ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg
     7201 cgagttgcgc gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga
     7261 tgccctgact gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt
     7321 cgcctctcct gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgc tgaacttggc
     7381 ttatccgagc gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat
     7441 cacgctgtat gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat
     7501 gcactatctg aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc
     7561 gtacagtctg gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt
     7621 cggcgagatg gaggtttggg cattggaagc atacggcgcg gcatacacgc tgcaagagat
     7681 gttgactgtg aagtctgacg acgtgaacgg tcgtaccaaa atgtacgaaa acatcgtcaa
     7741 aggcgaacac aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat
     7801 tcgctcactg ggcttggata tcgatttgga acgctactga tacgggtttc agacggcata
     7861 aggggagctg ttctgcaggt atgcggggca gccgacaatg tttaaaaacg aaatgccgtc
     7921 tgaaaacact gtacctctat ccatatcgaa aatccgccat gcggtaaaaa tacttccttc
     7981 aaggagcaaa aatgaatttg ttgaacttat ttaatccgtt gcaaactgcc ggcatggaag
     8041 aagagtttga tgccatcaaa atcggtattg cctctcccga aaccatccgc tcatggtctt
     8101 atggcgaagt caaaaaacct gaaaccatca actaccgtac gttcaaacct gagcgtgacg
     8161 gtttgttctg tgccaaaatc tttggcccgg tcaaagacta cgaatgcttg tgcggaaaat
     8221 acaaacgctt gaaatttaaa ggtgtaactt gtgaaaaatg cggcgtggaa gtaaccctgt
     8281 ccaaagtgcg ccgcgaacgc atgggccata tcgaattggc tgcgcccgtc gcacatattt
     8341 ggttcttaaa atccctgcct tcccgcttgg gtatggtgtt agacatgact ttgcgcgata
     8401 tcgaacgcgt attgtacttt gaagcatttg tggtaaccga tcccggtatg actccgctgc
     8461 aacgccgcca attgctgact gaagacgatt actacaacaa gctggacgaa tacggcgacg
     8521 atttcgatgc caaaatgggt gcggaaggta tccgcgaatt gctgcgtacc ctgaatgtag
     8581 cgggcgaaat cgaaatcctg cgccaagagt tggaatcgac cggttccgac accaaaatca
     8641 aaaaaatcgc caaacgcttg aaagtattgg aagctttcca tcgttccggt atgaaactgg
     8701 aatggatgat tatggatgtg ctgccggtat tgccgcctga tttgcgtccg ttggttccat
     8761 tggatggtgg tcgttttgcc acttccgatt tgaacgattt gtaccgccgc gttattaacc
     8821 gtaacaaccg tctgaaacgt ctgttggaac tgcatgctcc tgacatcatc gtccgcaacg
     8881 aaaaacgtat gttgcaagaa gcagttgact cgctgttgga taacggccgt cgcggtaaag
     8941 ccatgaccgg cgccaacaaa cgcccgctga aatcattggc agacatgatt aaaggtaagg
     9001 gtggccgctt ccgtcaaaac ctgctgggca aacgtgtgga ctactccggc cgttccgtga
     9061 ttaccgtagg cccgtacctg cgtctgcacc aatgcggttt gccgaaaaaa atggctttgg
     9121 aactgttcaa accgttcatt ttccacaaat tggaaaaaca aggtttggcc tctaccgtta
     9181 aagcagcgaa aaaattggta gagcaagaag taccggaagt atgggacatc ttggaagaag
     9241 tcatccgcga acatccgatt atgctgaacc gtgcgccgac cctgcaccgt ttgggtattc
     9301 aagcgttcga acctatcttg attgaaggta aagcgattca gttgcaccca ttggtgtgtg
     9361 ctgcgttcaa cgccgacttt gacggcgacc aaatggcggt acacgttcca ttgagcttgg
     9421 aagcacaaat ggaagcacgc acgctgatgc tggcttcaaa caacgtattg tctcctgcca
     9481 acggcgaacc gattatcgta ccttcccaag acatcgtatt gggcctgtac tacatgaccc
     9541 gcgaccgtat caatgccaaa ggcgagggca gcctgtttgc cgatgtgaaa gaagtgcatc
     9601 gcgcatacca taccaaacag gttgaactgg gtacgaaaat taccgtacgt ctgcgcgaat
     9661 gggtgaaaaa cgaagcaggt gagtttgagc ctgtcgttaa ccgttacgaa acaaccgtcg
     9721 gccgtgcatt gttgagcgaa atcctgccga aaggcctgcc gtttgaatat gtcaacaaag
     9781 cgttgaagaa aaaagaaatt tctaaactga ttaacgcatc gttccgcctg tgcggcttgc
     9841 gcgatacggt tatctttgct gaccacctga tgtacaccgg tttcggattt gcggcaaaag
     9901 gcggtatttc cattgccgtt gacgatatgg aaattccaaa agaaaaagcg gccttgctgg
     9961 ctgaagccaa tgccgaggtt aaagaaatcg aagaccaata ccgtcaaggt ttggttacca
    10021 acggcgaacg ctacaacaag gtggtcgata tttggggtcg tgccggcgat aagattgcta
    10081 aagcgatgat ggacaacttg tccaaacaaa aagttatcga ccgtgacggc aacgaagtcg
    10141 atcaagagtc attcaactcc atttatatga tggcggactc cggtgcccgt ggttctgcag
    10201 ctcagattaa acagttgtcc ggtatgcgtg gcttgatggc aaaacctgac ggctcgatta
    10261 ttgaaacgcc gattacctca aacttccgtg aaggtctgac cgtattgcaa tactttattg
    10321 cgacccacgg tgcgcgtaag ggtttggcgg ataccgcatt gaaaaccgcg aactccggtt
    10381 acctgactcg tcgtctggta gacgtaactc aagatttggt cgttgttgaa gacgattgcg
    10441 gtacttcaga cggctttgtc atgaaggcag tggtacaagg cggtgatgtg attgaagcat
    10501 tgcgcgatcg tattttgggt cgtgttaccg cgtctgacgt tgtcgatccg tcaagtggcg
    10561 aaaccttggt tgaagccggt acgttgctga ctgaaaaact ggtggatatg atcgaccaat
    10621 ccggtgtcga tgaagtcaaa gtccgtacgc cgattacttg taaaacccgt cacggcctgt
    10681 gtgcacactg ttacggtcgt gacttggcac gcggcaaact ggttaacgcc ggtgaggcag
    10741 tcggtgtgat tgctgcacaa tccattggcg aaccgggtac ccagttgacc atgcgtacgt
    10801 tccacatcgg tggtgcggca tcccgtgcgg cagcagccag ccaagtggaa gccaaatcca
    10861 acggtacggc acgattcagc agccagatgc gctacgttgc caacaacaaa ggcgagttgg
    10921 ttgtcatcgg ccgttcttgt gaagtcgtga ttcacgacga tatcggccgt gaacgcgaac
    10981 gccacaaagt accttacggt gccatcctgc tggtacaaga cggtatggcc attaaagccg
    11041 gtcaaacctt ggcaacctgg gatccgcata cccgtccgat gattaccgaa cacgcaggta
    11101 tggtgaaatt cgaaaacgtg gaagagggcg ttaccgttgc caaacaaacc gatgatgtaa
    11161 ccggtttgtc cactttggtg gtgattgacg gtaaacgtcg ttcctctagt gcttccaaac
    11221 tgctgcgtcc gactgtgaaa ctcttggacg aaaacggcgt ggaaatctgt attcccggta
    11281 cgtccactcc ggtttcgatg gcattccccg ttggtgcggt gattaccgta cgcgaaggtc
    11341 aggaaatcgg taaaggcgac gtattggcgc gtatcccgca agcctcttcc aaaacccgcg
    11401 acattaccgg tggtctgccg cgcgttgccg aattgtttga agcacgcgtg ccgaaagatg
    11461 ccggtatgtt ggcggaaatt accggtaccg tttccttcgg caaagagacc aaaggcaagc
    11521 aacgtctgat tgttactgac gtggacggtg tagcatacga gaccttgatt tccaaagaga
    11581 aacaaattct ggtacacgac ggtcaagtgg taaaccgcgg tgaaaccatc gtggacggag
    11641 ctgttgatcc gcatgatatt ctgcgtttgc aaggtatcga agcactggca cgctacattg
    11701 tccaagaggt gcaagaggtt taccgtctgc aaggtgtgaa gatttctgat aaacacatcg
    11761 aagtcatcat ccgtcaaatg ttgcgccgtg tgaacattgc ggatgccggc gaaaccgggt
    11821 tcattaccgg agagcaggtc gaacgcggcg atgtgatggc ggccaatgaa aaagctttgg
    11881 aagaaggcaa agagccggca cgttacgaaa acgtattgct gggtattacc aaagcctccc
    11941 tgtccaccga cagtttcatt tctgccgcat cgttccaaga aacgacccgt gttctgaccg
    12001 aagccgcgat tatgggcaaa caagacgaac tgcgcggttt aaaagagaac gtcatcgtcg
    12061 gccgcctgat tcctgccggt accggtttga cttatcaccg cagccgtcat cagcaatggc
    12121 aaggggtaga gcaggagact gctgaaaccc aagtaacgga tgaataatct tcggcgcgtt
    12181 tattcaatac aaaaccgcaa gctttaagct tgcggttttt ctttgtccga ttaaggcaaa
    12241 aacaagcgtt ttcgtcattt tgaggcgtgt ggattattcc ttaggtattt tcgggccgga
    12301 gaccaacgag gtggcgggtg tcgtcggtac gtccggagac caaaataact ttgccaagga
    12361 tgttggtttc ggcggtcaaa aaagtagcgt cttaatgttt tccatttaaa caaatgtcgt
    12421 ctgaaacttc agacggcatt tcctttaaaa aataaatatg aaacccagaa atctcttttt
    12481 tgcaggctgc ctgctgactt cggcgacgtt tgccgaggat atcggcgtac ctgtcgaact
    12541 gattaacgtc ggtaatcgga ttgcgatgcc gtctgaaggg gaaagcctcg ccctcctgcc
    12601 gtttgccgag gatgtaccgc cggttcgcga tgcaatgccg tctgaagttc ctaaaagcgc
    12661 ggcaggcggc gatgttcggg gtgaccggat gggaatgccg attaacatcg gatgagcgcg
    12721 gctttatggc ataaaaaact gtcgtggaaa ggatttacac cccaaataaa tttccgttac
    12781 aacaagatca acagcaatat gcccgccttt tattcgcgca gcagcaagga atggtttgtc
    12841 agtatagaaa aaacgtattg acagtatttt ctccagccgt ccgactgatt gtgagggatg
    12901 tcggtaaata tttatcggca aacaagaaaa tcatctttct tcttgtcgtt atgcttgact
    12961 gtatacttgc aataaaaata taattccact cttgccgaca tggtgtcggc aagtatttaa
    13021 ctcaacagga cgagaaaata tgccaactat caaccaattg gtacgcaaag gccgtcaaaa
    13081 gcccgtgtac gtaaacaaag tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg
    13141 cacccgtgta tacacgacta cccctaaaaa acctaactct gcattgcgta aagtatgtaa
    13201 agtccgcctg accaacggtt ttgaagtcat ttcatacatc ggcggcgaag gccacaacct
    13261 gcaagagcac agtgtcgtat tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg
    13321 ttaccacact gtacgcggtt ctttggatac tgcaggtgtt aaagaccgta aacaagcccg
    13381 ttccaaatac ggtgctaagc gtcctaaata attactggga cttaaatagg cacgtcggcc
    13441 gcctaagctg aacaacggcc gagtaagtga atactcaatt gggtattcat gggaatagac
    13501 ccgactgaat agattaaagg aaattaaaat gccaagacgt agagaagtcc ccaagcgcga
    13561 cgtactgcca gatcctaaat tcggcagcgt cgagttgacc aaattcatga acgtattgat
    13621 gattgacggt aaaaaatccg ttgccgagcg tatcgtttac ggtgcgttgg aacagattga
    13681 gaaaaaaacc ggcaaagtag caatcgaagt atttaacgaa gccattgcaa acgccaaacc
    13741 tatcgtggaa gtgaaaagcc gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt
    13801 tcgtccttca cgccgtttgg ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg
    13861 tggtgagaaa tccatggacc tgcgtttggc aggcgaattg attgatgcgt ccgaaggccg
    13921 tggcggtgcg ttgaaaaaac gtgaagaagt acaccgtatg gctgaagcca acaaagcatt
    13981 ctctcacttc cgtttctaat tttgaaaggc taataaaatg gctcgtaaga ccccgatcag
    14041 cctgtaccgt aacatcggta tttccgccca tatcgatgcg ggtaaaacca cgacgacaga
    14101 acgtattttg ttctataccg gtttgaccca caagctgggc gaagtgcatg acggtgcggc
    14161 tactaccgac tacatggaac aagagcaaga gcgtggtatt accattacct ctgctgccgt
    14221 tacttcctac tggtccggta tggcgaaaca attccccgag caccgcttca acatcatcga
    14281 caccccggga cacgttgact ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg
    14341 cgcggtaatg gtttactgcg cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg
    14401 gcaagccaac aaataccaag tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg
    14461 tgccaacttc ttccgcgttg tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc
    14521 tatcgtcatt ccggttggtg cggaagacaa cttcagcggt gtggttgatt tgttgaaaat
    14581 gaaatccatc atttggaatg aagtcgataa aggtacaacc tttacctatg gcgatattcc
    14641 tgccgaattg gtcgaaactg ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga
    14701 agccagcgaa gaactgatgg acaaatactt aggcggcgac gagctgaccg aagaagaaat
    14761 cgtaggcgcg ttgcgtcaac gtactttggc aggcgaaatt cagcctatgc tgtgtggttc
    14821 tgcatttaaa aacaaaggtg ttcaacgtat gttggacgca gttgtagaat tgctgccagc
    14881 tcctaccgat attcctccgg ttcaaggtgt caacccgaat accgaggaag ccgacagccg
    14941 tcaagccagc gatgaagaga aattctctgc attggcgttc aaaatgttga acgacaaata
    15001 cgtcggtcag ctgaccttta tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt
    15061 attgaactcc gtaaaaggca ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc
    15121 agaccgtact gaaatcgaag aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa
    15181 agacgttact accggtgaaa ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat
    15241 ggaattcccc gagccggtaa tccatattgc cgttgagccg aaaaccaaag ccgaccaaga
    15301 gaaaatgggt atcgccctga accgcttggc taaagaagac ccttctttcc gtgtccgtac
    15361 agacgaagaa tccggtcaaa ccattatttc cggtatgggt gagctgcact tggaaattat
    15421 tgttgaccgt atgaaacgcg aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc
    15481 ttaccgtgaa actatccgca aagccgttaa agctgaatac aaacatgcaa aacaatccgg
    15541 tggtaaaggt caatacggtc acgttgtgat tgaaatggaa cctatggaac cgggtggtga
    15601 aggttacgag tttatcgatg aaattaaagg tggtgtgatt cctcgcgaat ttattccgtc
    15661 tgtcgataaa ggtatccgcg atacgttgcc taacggtatc gttgccggct atcctgtagt
    15721 tgacgtacgt atccgtctgg tattcggttc ttaccatgat gtcgactctt cccaattggc
    15781 atttgaattg gctgcttctc aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct
    15841 gcttgagcca atcatggcag ttgaagtgga aaccccggaa gaatacatgg gcgacgtaat
    15901 gggcgacttg aaccgccgtc gcggtgttgt attgggtatg gatgatgacg gtatcggcgg
    15961 taaaaaagtc cgtgccgaag tacctctggc agaaatgttc ggttattcga ccgacctgcg
    16021 ttctgcaacc caaggccgcg ctacttactc tatggagttc aagaaatatt ctgaagctcc
    16081 tgcccacata gctgctgctg taactgaagc ccgtaaaggc taatcaggca aataggccgt
    16141 ccgaaaggct gaaatgattt ttcagacggc attgttcttt aatcgatctt taatgtaaag
    16201 gaattagc
//
LOCUS       DAWFIT010000040        15938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFIT010000040 DAWFIT010000000
VERSION     DAWFIT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.040"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..79)
                     /locus_tag="WGQ85_001193"
     CDS             complement(<1..79)
                     /locus_tag="WGQ85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0750624.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGQ85_001194"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGQ85_001194"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0750625.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGQ85_001195"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGQ85_001195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0750626.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGQ85_001196"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGQ85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0750627.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQSERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WGQ85_001197"
     CDS             4804..5868
                     /locus_tag="WGQ85_001197"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0750628.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5888..6850
                     /locus_tag="WGQ85_001198"
     CDS             5888..6850
                     /locus_tag="WGQ85_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750629.1"
                     /translation="MPSEPPFGRYLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTTFCCLSLTAALILTACSSGGGGVAADIGAGLADVLTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTALQTEQEQDLEHSRKMVAKRRFKIGDIAGEHTSFDKLPKDVMATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAVAYIKPDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGEKAQEVAGSAEVKTANGIHHIGLAAKQ"
     gene            6990..7667
                     /gene="tsaB"
                     /locus_tag="WGQ85_001199"
     CDS             6990..7667
                     /gene="tsaB"
                     /locus_tag="WGQ85_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0750630.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AVASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRTADIALPEGHVFSDG
                     IGNAFALENRPPFAGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTVKEQ
                     AERKART"
     gene            7664..8104
                     /gene="rimI"
                     /locus_tag="WGQ85_001200"
     CDS             7664..8104
                     /gene="rimI"
                     /locus_tag="WGQ85_001200"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0750631.1"
                     /translation="MNIRRPSPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8098..8832
                     /locus_tag="WGQ85_001201"
     CDS             8098..8832
                     /locus_tag="WGQ85_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0750632.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            8894..9535
                     /gene="pyrE"
                     /locus_tag="WGQ85_001202"
     CDS             8894..9535
                     /gene="pyrE"
                     /locus_tag="WGQ85_001202"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0750633.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9609..10043
                     /locus_tag="WGQ85_001203"
     CDS             9609..10043
                     /locus_tag="WGQ85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750634.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10040..11350
                     /gene="argA"
                     /locus_tag="WGQ85_001204"
     CDS             10040..11350
                     /gene="argA"
                     /locus_tag="WGQ85_001204"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0750635.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11446..13461
                     /locus_tag="WGQ85_001205"
     CDS             11446..13461
                     /locus_tag="WGQ85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0750636.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEARELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNNCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13575..14531)
                     /locus_tag="WGQ85_001206"
     CDS             complement(13575..14531)
                     /locus_tag="WGQ85_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750637.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14767..15732
                     /locus_tag="WGQ85_001207"
     CDS             14767..15732
                     /locus_tag="WGQ85_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750638.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         3550 a         4844 c         4369 g         3175 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacaac cggtttcata cacatctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaacc
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctta tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg caacgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatatc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caataccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt atagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccgta ttggcgcatg gtgtgcatct ggtttttgcg tccggtaagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgttg
     3421 tacacatagt gcagggcgat ggtcagctcg accgcaccta agttgctggc gaaatgaccg
     3481 ccggtttgac cgacggattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagtgct
     4261 ttgggtacgc gctcggactg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggca gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaaccggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat ttacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatacctgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgccggt
     5881 aaacaaaatg ccgtctgaac cgccgttcgg acggtatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagta aacctgtgaa ccgaactacc ttctgttgcc tttctttgac
     6061 cgctgccctg attctgaccg cctgcagcag cggagggggc ggtgtcgccg ccgacatcgg
     6121 tgcggggctt gccgatgtgc taaccgcgcc gctcgaccat aaagacaaag gtttgcagtc
     6181 tttgacgctg gaccagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct taatacgggc aaattgaaga acgacaaggt
     6301 cagccgtttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caagtgtaca aacaaagcca ttccgcctta accgcccttc agaccgagca
     6421 agaacaagat ctagagcatt ccaggaagat ggttgcgaaa cgccggttca aaatcggcga
     6481 catagcgggc gaacatacat cttttgacaa gcttcccaaa gacgtcatgg cgacatatcg
     6541 cgggacggcg ttcggttcag acgatgccgg cggaaaactg acctatacta tagattttgc
     6601 tgccaaacag ggacacggca aaatcgaaca tttgaaatcg ccggaactca atgtcgatct
     6661 ggccgtcgcc tatatcaagc cggatgaaaa acaccatgcc gtcatcagcg gttccgttct
     6721 ttacaaccaa gacgagaaag gcagttactc cctcggtatc tttggcgaaa aagcccagga
     6781 agttgccggc agcgcggaag tgaaaaccgc aaacggcata caccatatcg gccttgccgc
     6841 gaagcaataa ccattgtgga aatgccgtcc gaacacgata atttaccgtt cggacggcat
     6901 ttttgcgcca taccgcccta ggacacgacg gcacggcagg caagccggtt tcactatata
     6961 atgccgtctg aaccaacgag aaccacacca tgcaagccga tttcaaccgc cccgtcctcg
     7021 ccgtcgatac cggaacttcc tatttgtcgc tcgcgctgcg tgccgacggc gaaacccgtc
     7081 tgttccatca ggaagccggc agccgccagt ccgaactgat tctgccggaa atccgcacgc
     7141 tgtttcacga tgcgggcatt accgccgccg atttgggggc gattgtttat gcgaaaggcc
     7201 ccggcgcgtt taccggactg cgtatcggta tcggcgtggc gcaaggtttg gcaacgccgt
     7261 tcaatacgcc tttaatcggc atccccacgc tcgatgccgt cgcctcgctg ccgccgccgc
     7321 aaagctgcat actcgccgct gcggacgcac gtatgggcga agtgttttat gcgtggttcg
     7381 acacgctgaa ccgccgccgt ttgagcgatt atcaagtcgg taggacggca gacattgccc
     7441 tccccgaggg acacgtcttt tcagacggca tcggcaatgc gttcgcgctg gaaaaccgtc
     7501 cgcccttcgc aggcaaaccc gatatgccga ccgccgccga ttttctcaat ctggcggaag
     7561 aaggcggttt tcccgccgtc gctgcggcac acgccgagct gctctacgtc cgcaacaaaa
     7621 tcgccttgac tgtcaaagaa caggcggaac ggaaagcccg aacatgaaca tccgccgccc
     7681 ctcaccgtcc gactgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg
     7741 gacgcaacgc caatttgagt ccgcactggt ttcgccgtcc gaacaggttt tccttgcgga
     7801 aaaagacggc gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact
     7861 gcacctgatt gccaccgcgc ccgaatgccg ccgccaagga attgcgtccg ccctgctcga
     7921 atattggtac gcacacctgc ccgaaggcac gcaacgcctg ctgctcgaag tccgcgcagg
     7981 caacaccgcc gcacaggcac tgtacgcggc gcacggcttc agcattgcgg gcaggcggaa
     8041 aaactattac cgtacagccg acggtaaaac cgaagatgcc gtcttgatgg agaaaatatg
     8101 ttaagcgcgc gctacctcca cctgcacgaa gctttgggtt taggcccgat gtggctgaaa
     8161 caggccgccg ccgtcctgcc gcccaaaaac acacccgcac cctcggcaca ggcacgtcct
     8221 caaaccgtcc gcgccgcccc gatccgccct tcccaacccc ataacggtca ggcacggctc
     8281 gaaacgatga aagcgttgga aaccgccgcc gtacatacgc gcaaacccgc gcctgaaacc
     8341 gaaacgcctc tgcccggcct ttcagacggc atcgcccccg ttcccgccgc ttcgggcatc
     8401 accaagcttg ccgtcgtcag cctttgccca ccgatcgagg atgcggttta cgggcaactg
     8461 ttccacggca aagcaggtgt tctgcttgac aatatactca aagccgtagg actggatgcc
     8521 gcttatgtac acaagaccag ctgggtgaaa accgccgccg tcggcaaccc gatgccgtct
     8581 gaacaggccg tcgcgaatgc gctgggtcaa atcgccaggg aactcgacgg ctgccgtgcc
     8641 cccgccgtcc ttttcctcgg gcaggctttt gtccagcctg aacggcaaac gatgattgaa
     8701 actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgctcggct tttacgccaa
     8761 cccgaactta aagcccgaaa ctggcaggtg ttgaaacagt tgaaacacgc cttgcaggca
     8821 ggcggcagtt gaagcaggcc gcacggggcg gtagaatcgc aactgcgtcc caatatctga
     8881 cagaaagcac aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa
     8941 tgttttgaaa ttcggcgaat tcaccaccaa agccggacgg cggtcgccct atttcttcaa
     9001 tgccggcctc tttaacgacg gcttgtccac gctgcaactg gcaaaattct atgcacaatc
     9061 catcattgaa agcggcatcc gattcgatat gctgttcggt cccgcctaca aaggtattat
     9121 tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta
     9181 caaccgcaaa gaagccaaag accacggcga aggcggcgtg ttggtcggcg cgccgcttaa
     9241 agggcgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat
     9301 caaactgatt gaagcggagg gtgcaacccc cgccggtgtc gccatcgcgc tcgaccgcat
     9361 ggaaaaaggc acgggtgaat tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc
     9421 cgtcgccccc atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt
     9481 cggacagttc ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca
     9541 aagcatatgc cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag
     9601 gaaataccat gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa
     9661 cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag
     9721 ccaaagacca tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt
     9781 cggatgtcaa actcgttcac cgcatcggca cgcgcgccat tggcaagaaa cagatttccc
     9841 gcgacgaaat cgccggcatc ctcaacggcg gtacaaccct gcacgatacg ccgcccgcaa
     9901 ccgccgccac ccctgccgcc gcaccgcagg ttgccgtacc gcccgccgcg cctgcccgtc
     9961 aggaagggtt caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc
    10021 tttcctacct cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc
    10081 cgtcccctac atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct
    10141 gctcgaaggt gataccttaa acaagctcgc cgccgacatc gggctgttgt cgcaactggg
    10201 catcaggctc gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca
    10261 aggccgcacg ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca
    10321 ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt
    10381 ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg
    10441 tccgataggt gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga
    10501 caccgccgcc ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg
    10561 acattcctac agcggcaaga ccttctatct cgatatgctt caaaccgccg cctccgtcgc
    10621 cgtctcgctt caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc
    10681 cgacggcacg ctcgccgaaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc
    10741 cggcggcgaa acgcgacggc tgatttcgtc caccgttgcc gcgctcgaag gcggcgtgca
    10801 tcgcgtccaa atcctcaacg gtgcctccga cggcagcctg ctgcaagaac tcttcacccg
    10861 caacggcatc ggcacatcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag
    10921 cggcgacatc ccgcacatcg ccgccctcat ccgcccgctg gaagaacagg gcatcctgct
    10981 gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga
    11041 cggcaacctg tacggttgcg ccgccctgaa aacctttgcc gaagccgatt gcggcgaaat
    11101 ctcctgcctt gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc
    11161 ccacattatc gataaggcgc gcagtatggg cataagcagg ctgttcgccc tctccacaaa
    11221 taccggcgaa tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga
    11281 aacgcggcgc aaagactacc gcagcaacgg acgaaactcg catattctgg tacgtcgcct
    11341 gcaccgctga ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg
    11401 gcatttcccc gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc
    11461 gacccctacc gccattttga aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa
    11521 gcgaatgccg aaacgcgcgc gcgtttttta aacaacgaca aggcgcgcgc gctttcagac
    11581 ggcattttga atcaaatgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg
    11641 cggatgtacc atttccatca ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgcacg
    11701 gcggcgacct accgttcggg ctatccggag tggaaaatcc tgttttcagt ggcggacttc
    11761 gacgaattgc tcggcgacga tgtatatttg ggcggcgtgt cgcacttggt ggaaaagccc
    11821 aaccgcgcgt tgttgacact aagcaaatca ggcggcgata cggcgtacac gctggaagtg
    11881 gatttggaag caagggaatt ggtagaaggt ggttttcact ttccggcagg caaaaaccaa
    11941 gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg cttgggacga acgccagttg
    12001 accgaatcgg gctatccgcg tgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa
    12061 agcctgcctg tgtatcaaat cgataaagat gcaatgatgg tgaacgcgtg gcgttatctc
    12121 gatccgcagg gttcgccgat tgatttgatt gaagcgtcgg acggttttta caccaaaacc
    12181 tatttgcagg tgtcggccga aggcgaggcg aaaccgttaa acctgcccaa caattgcgat
    12241 gtggtcggct atctggcggg gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg
    12301 aaccaaagct atccgagcgg tgcgctggtg gcggtgaagc tgaatcgggg tgaactcggg
    12361 gcggcgcagc ttttgtttgc gcctgatgaa acgcaggcat tggaaagcgt ggaaacgacc
    12421 aagcgttttg tggtggcgag cctgctggaa aacgtacaag gccgtctgaa agcgtggcgt
    12481 tttgccgacg gcaaatggca ggaagtcgaa ctgccgcgcc tgccttcggg cgcgttggaa
    12541 atgaccgacc agccttgggg cggcgacgtg gtttaccttg ccgccggcga tttcaccaca
    12601 ccgctgacgc tgtttgcgct ggatttgaac gtgatggaac tgaccgtcat gcgccgccag
    12661 ccgcagcagt tcgattcaga cggcatcgaa gtgcggcagt tttgggtgat gtcgtccgac
    12721 ggcgaacgca tcccttattt ccacgtcggc aaaaacgccg cgcccgacac gccgacgctg
    12781 gtttatgcct acggcggttt cggcgtaccc gagctgccgc attatctggg cagcatcggc
    12841 aaatactggc tggaagaggg caacgccttc gtgttggcga acatacgcgg cggcggcgag
    12901 ttcggcccgc gctggcatca ggcggcgcag ggaatcagca aacacaaaag cgtcgatgat
    12961 ttgcttgccg tcgtgcgcga tttgtccgag cgcggcatca gttcgcccca acacatcggt
    13021 ttgcagggcg gcagcaacgg cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa
    13081 agcataggcg cactggtgtg cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc
    13141 tccgccggtt caagctggac agacgaatac ggcaatccgc aaaaatacga agtctgcaaa
    13201 cgccggttgg gcgaattgtc gccgtatcac aatctttcag acggcatcga ttatccgccc
    13261 gcgctcatca ccaccagcct cagcgacgac cgcgtccatc ccgcccacgc gctcaagttt
    13321 tacgccaaac tgcgcgaaac ctcgccgcaa tcttggctct actcgcctga cggcggcggc
    13381 cataccggca acggcacgca gcgcgaagcc gccgacgaac tcgcctgcgt gttgctgttt
    13441 ttgaaagagt ttttgggcta agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt
    13501 cgtctgaaac tgctttttca gacgaccttt tttaatggtt gtttcaaatc aaaatatcta
    13561 tgccgccggc cccatcagca cttcttcaca tccgaaggca aaaatccgta atgccgtctg
    13621 aacgcttcgt tgaaccgtcc cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg
    13681 gtgctgccgg attcgatgag ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg
    13741 gcgacggttt cgccggtttg cgctttgaaa tagcgtttca ggtagcattc gttcagtccg
    13801 acgcggcggg cgatttcggc gatggtcagc ggacgggcga attcgtgttg caggatgtcg
    13861 gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc
    13921 aataatcggg cggagagttc cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc
    13981 gattcgaacg ggcgttgcag cagtgggcag gccgccgccg tcagtgccgc cgcgttttgc
    14041 gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc
    14101 aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac
    14161 atgccgctgt tgtcgggcag cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc
    14221 cagatttcgc cggcagacgg gcggtattcc ctgccgccca tttgtaaccg gttctgcccc
    14281 gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg
    14341 cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac
    14401 atttccagat gcaaaccggg ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg
    14461 tccaacgccc agccggattt atcggagcgg acataggttt ggtactggcg gtagatggcg
    14521 gcggtgttca tgattggata ggaacgagtt gtctaacaaa tgaattaaat aggaattatt
    14581 accaataatc aagcgcaggg attggttgaa acggaaaagg tcgtctgaaa gggtgtttca
    14641 gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg ggaattttgc gttttgcggc
    14701 gtggtttctg caggttgttt gcttaataat aaacattctt attcgtatgc aaaggaaccg
    14761 cacaccgtga aaccgcgttt ttattgggca gcctgcgccg tcctgctgac cgcctgttcg
    14821 cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg catctgccgc cacactgacc
    14881 gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac
    14941 gactgggcgg cgttggatac gctgaccgaa ttgggcgtga atgtgggcgc aaccaccgcg
    15001 ccggtgcgcg tggattattt gcagcctgca tttgacaagg cggcaacggt ggggacgctg
    15061 ttcgagcccg attacgaagc cctgcaccgc tacaatcctc agcttgtcat taccggcggg
    15121 ccgggcgcgg aagcatatga acagttggcg aaaaacgcga ccaccataga tctgacggtg
    15181 gacaacggca atatccgcac cagcggcgaa aagcagatgg agaccttggc gcggattttc
    15241 ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg
    15301 cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt cggttacggg caacaaggtg
    15361 tccgccttcg gcacgcagtc gcggttggca agttggatac acggcgacat cggcctaccg
    15421 cctgtagacg aatctttacg caacgagggg cacgggcagc ctgtttcctt cgaatacatc
    15481 aaagagaaaa accccgattg gattttcatc atcgaccgta ccgccgccat cgggcaggaa
    15541 gggccggcgg ctgtcgaagt attggataac gcgctggtac gcggcacgaa cgcttggaag
    15601 cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg tcgcgggcgg ctcgcggcag
    15661 ttgattcagg cggcggagca gttgaaggag gcgtttgaaa aggcagaacc cgttgcggcg
    15721 gggaaagagt agggagtcgt ctgaaaacgg agtttccgaa ggaagcgggg ggtttctgcg
    15781 aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa
    15841 gcaaacctac ggtttgccgc cctctcccta gccctctccc acagggagag gggattgggg
    15901 tgcaggctgc ctttaaggtt taggcaaatt tttaactt
//
LOCUS       DAWFIT010000041        15811 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFIT010000041 DAWFIT010000000
VERSION     DAWFIT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.041"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            98..1123
                     /locus_tag="WGQ85_001208"
     CDS             98..1123
                     /locus_tag="WGQ85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0750639.1"
                     /translation="MKYKALSLLPLVAALAACAGGGVAEPHVPVSIPTATPLPKGEVT
                     LSSDNGKIENINTTSTGSTSVISIQERAVTKNYFGVESQEKSFIFKTPGGAQYTLSSY
                     ADPITVSYSSPDFKIPDHHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIG
                     PNGEIDLFAGGFPVGKTPPPAFSYGSSTPETKGTGKITYQVWGIRVRNGQFVTSSYTP
                     PKNSGYTFNPTNTPVLSFITANFNSNKLAGKIIGNSDYGPDVEIKEAQIDGLSFSGDA
                     TSGGKTGKLEGKFFGKFNSSYDSDTSIGGKITFDGDRSLDTVFGGVSYKKELESTTDR
                     ETTHLTK"
     gene            1154..3583
                     /gene="hupB"
                     /locus_tag="WGQ85_001209"
     CDS             1154..3583
                     /gene="hupB"
                     /locus_tag="WGQ85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0750640.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENDGYVFDPTNPSPRRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESRS
                     KYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDNPKYTTAIQGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAKN
                     WELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
     gene            complement(4190..5824)
                     /gene="groL"
                     /locus_tag="WGQ85_001210"
     CDS             complement(4190..5824)
                     /gene="groL"
                     /locus_tag="WGQ85_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0750641.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5917..6207)
                     /locus_tag="WGQ85_001211"
     CDS             complement(5917..6207)
                     /locus_tag="WGQ85_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0750642.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            6436..7089
                     /locus_tag="WGQ85_001212"
     CDS             6436..7089
                     /locus_tag="WGQ85_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0750643.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            7302..8837
                     /locus_tag="WGQ85_001213"
     CDS             7302..8837
                     /locus_tag="WGQ85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0750644.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            8834..8926
                     /locus_tag="WGQ85_001214"
     CDS             8834..8926
                     /locus_tag="WGQ85_001214"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0750645.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(8986..10206)
                     /gene="lysA"
                     /locus_tag="WGQ85_001215"
     CDS             complement(8986..10206)
                     /gene="lysA"
                     /locus_tag="WGQ85_001215"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0750646.1"
                     /translation="MTLFCEQVPYPRLAEALGTPLYVYSQSALTEAFEHYQTAFAALS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IKFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLIEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(10217..10387)
                     /locus_tag="WGQ85_001216"
     CDS             complement(10217..10387)
                     /locus_tag="WGQ85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0750647.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSTPQSAPPTQK"
     gene            10458..10781
                     /gene="cyaY"
                     /locus_tag="WGQ85_001217"
     CDS             10458..10781
                     /gene="cyaY"
                     /locus_tag="WGQ85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0750648.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDNRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            10808..11824
                     /locus_tag="WGQ85_001218"
     CDS             10808..11824
                     /locus_tag="WGQ85_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0750649.1"
                     /translation="MNTRPFYFGLIFIAIIAIFANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGTLLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            11850..12269
                     /locus_tag="WGQ85_001219"
     CDS             11850..12269
                     /locus_tag="WGQ85_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0750650.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALSINDYIHAVYDFVEQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            12291..12797
                     /gene="luxS"
                     /locus_tag="WGQ85_001220"
     CDS             12291..12797
                     /gene="luxS"
                     /locus_tag="WGQ85_001220"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0750651.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            12943..15738
                     /gene="polA"
                     /locus_tag="WGQ85_001221"
     CDS             12943..15738
                     /gene="polA"
                     /locus_tag="WGQ85_001221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0750652.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         3784 a         4287 c         4029 g         3711 t
ORIGIN      
        1 cgatttttgc accgacccac aaaaccagtt gacatcatat aacatttaaa ctgataatca
       61 aaactattat taacacatca tcaagattga gtatgcgatg aaatacaaag ccctgtcctt
      121 actgccgctt gtcgctgccc ttgccgcctg tgccgggggg ggggtagccg aaccgcacgt
      181 ccccgtgtcc atccccaccg ccacgccgct gcccaaaggc gaggtaacgt tatcaagcga
      241 taacggcaaa atcgaaaaca tcaacaccac cagcaccgga agcacatccg tcatttccat
      301 tcaagagcgg gcggttacaa aaaactattt cggtgttgaa tcacaagaaa aatcattcat
      361 cttcaaaaca cccggcggcg cgcaatacac cctttcatcc tacgccgacc ccataaccgt
      421 cagctactcc tcccccgatt tcaaaattcc cgaccaccac gcagggcagc ggcttgccga
      481 cggcagccgc atctttatct gctgcagcga ctccggggct acttcgtatg cggaaattac
      541 caaacaagat tatatgaaat tcggggcgtg gataggtccc aacggcgaaa tcgacctctt
      601 cgccggcggc ttccccgtcg gcaaaacgcc acctcctgcg ttctcctacg gcagcagcac
      661 acccgaaacc aaaggcacag gcaaaatcac ttatcaggtt tggggcatcc gcgtcagaaa
      721 cgggcaattt gttacttcct cttatacgcc gccgaaaaac agtggttata cgttcaatcc
      781 taccaacacc cccgtccttt cctttatcac cgccaatttc aacagtaaca aactggcagg
      841 caaaatcatc ggcaacagcg attacgggcc ggatgtggaa attaaagaag cacagattga
      901 cggattatcc ttttccggcg atgccacctc cggcggcaaa accggcaaat tggaaggcaa
      961 gttttttggc aaattcaaca gcagctatga cagcgacacc agcataggcg gcaaaatcac
     1021 ctttgacggc gaccgctccc tcgataccgt attcggcggc gtgagttata aaaaagaact
     1081 tgaaagcacc accgatcgag agaccaccca tctcacaaaa taataattaa tccgctttta
     1141 aaaaggtttt cttatgccca tccccttcaa acccgtattg gctgccgccg ccatcgccca
     1201 agcgtttccc gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc tgaacgaaat
     1261 caccgttacc ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc gccgcaaaac
     1321 cttagacaag ctcttggtca acgacgaaca cgacctggtg cgctacgacc ccggcatttc
     1381 cgtcgtcgaa ggcggcaggg cgggttctaa cggctttacc atacgcggcg tggacaaaga
     1441 ccgcgtcgcc atcaacgttg acgggctggc gcaggcggaa agccgctctt ccgaagcctt
     1501 ccaagagttg ttcggcgcgt acggcaactt caacgccaac cgcaacacct ccgagccgga
     1561 aaacttttcc gaagtaacca tcaccaaagg cgcggactcg cttaaatccg gcagcggcgc
     1621 attgggcggc gcagtcaatt accaaaccaa atccgcaagc gattatgttt ccgaagacaa
     1681 gccctaccat ttggggataa agggcggcag cgtcggaaaa aacagccaaa aattcagcag
     1741 catcaccgcc gccggcaggc tctttggttt ggatgcctta ttggtttata cccgccgctt
     1801 cggcaaagaa accaaaaacc gctcgaccga gggcgatgtc aaaattgaaa acgacggata
     1861 tgtctttgac ccaaccaacc cctcgcccag aagatacctt acctacaaag ccacaggggt
     1921 tgcgcgctcc caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt tcaagctggg
     1981 ctacaatttc aacgaccgca accgcatcgg ctggattttt gaagactccc gcaccgaccg
     2041 ttttaccaac gagctgtcta atttgtggac gggtacgacc acgtctgccg caacgggcga
     2101 ctaccgccac cgccaagacg tgagctaccg ccgccgctcc ggcgtcgaat acaaaaacga
     2161 attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat
     2221 gaacacttgg acttgggaca tcccgaaaaa ttacgatttg cgcggcatca acagtgaagt
     2281 ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa
     2341 acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttgggcg gcggcagagg
     2401 ggacaatgcc aactcggatt acagctattt cgtaaaactg tacgacccca aaatcttcac
     2461 ttccaaccaa gccaaaatca cgatgctgat cgaaagccgg tcgaaatata aatttgccta
     2521 ttggaacaat gcgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg
     2581 ctacgacaaa aacagcagca gcgcgaaaga caatccgaaa tacaccaccg ccatccaggg
     2641 gcagattccc catttgggtt cggaacgtgc gcacgccggc ttcagctacg gcacggggtt
     2701 cgattggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc
     2761 gccgacttcg gacgaaactt ggctactgtt cccacacccc gatttctacc tgaaagccaa
     2821 cccaaacctg aaagccgaaa aagccaaaaa ctgggaattg ggtctggcgg gcagcggcaa
     2881 agcgggcaac ttcaagctct cgggcttcaa aaccaaatac cgcgacttta tcgaattgac
     2941 gtatatgggc gtttcgtcag acgataaaaa caaccccaga tacgccccgc tttcagacgg
     3001 cacggcattg gtcagctcgc ccgtttggca aaaccaaaac cgctccgccg cctgggtgaa
     3061 aggcatagag tttaacggca cgtggaacct cgacagtatc ggtttgccca aagggctgca
     3121 caccggcctc aacgtcagct acatcaaggg caaggcaacg caaaacaacg gcaaagaaac
     3181 gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc
     3241 caaacgttgg ggcatcaacg cctacgccac gcgcaccgcc gccaaaaagc cgtccgacac
     3301 cgtccacagc aacgacgact tgaacaaccc gtggccttat gccaaacaca gcaaggccta
     3361 tacgctgttc gacctttccg cctacctcaa catcggcaaa caggttacgc tccgcgccgc
     3421 cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca gcatccgcga
     3481 gttcggcacg gtcaaccgcg ttgacaacaa aacccacgcc ggcatccaac gctttacctc
     3541 gccgggcagg agctacaatt tcaccatcga agccaagttc taagctcctg atgtgccaat
     3601 gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc
     3661 ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg
     3721 tcattcccgc gactctccgt cattcccacg gaagtgggaa tctagaaacg caaagctgca
     3781 agaatttatc ggaaatgact gaaactcaac gaacctggat tcccgcctgc gcgggaatga
     3841 cgaagctatc cgcacagaaa cctgcaccac gtcattccca cgaaagtggg aatccagaac
     3901 gtaaaatctg aagaaaccgt tttatccgat acgtttccgc accgacagac ctagattccc
     3961 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
     4021 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaaca acaatgcaaa
     4081 gaacggtgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaccgc
     4141 acggcgttga ccgtgcggct tgttgtctga aggcttcaga cggcattgct tacatcatgc
     4201 cacccatacc gcccataccg cccatgtcag gcatagcagg tttttcttca gggatttcgg
     4261 caatcataca atcagtggtc agcatcaggc cggcgataga tgcggcgtgt tgcagcgcgg
     4321 aacgggttac tttggcgggg tcgagtacgc ccatttcgat catgtcgccg tattcgccgg
     4381 agcctgcgtt gtaaccgtag ttgcctttgc cttccaacac tttgttcacc accacgctag
     4441 gttcgccgcc tgcgttggca acgatttggc gcagcggaga ctcaacggcg cgcaagacga
     4501 tttgtacgcc tgcgtcttgg tcggcattgc cggtgtgcag gttttccaga gcggcacggg
     4561 cgcgcaacag ggcgacaccg ccgcctgcaa ccacgccttc ttcaacggct gcgcgggtag
     4621 cgtgcagcgc gtcttccacg cggtcttttt tctctttcat ttcgacttcg gtcgcggcac
     4681 cgactttgat cactgccacg cctcctgcca atttggcaac gcgctcttgc agtttttctt
     4741 tgtcgtaatc gctggtcgcg gtttcgattt gttggcggat ttcggcaaca cgcgcttcga
     4801 tttgggctgc gtcgccgaag ccgtcgatga tggtggtgtt ttctttaccg atttcgatgc
     4861 gtttggcttg acccaagtcg tccaaagtcg ctttttccag agacaggccg acttcttcgg
     4921 aaatcaccac gccgccggtc aggatggcga tgtcttgcaa catcgctttg cggcggtcgc
     4981 cgaagccagg ggctttgacg gcaacggttt tcaggatgcc tcggatgttg ttcacgacca
     5041 aagtcgccaa ggcttcgcct tctacgtctt cagcgataat caacagcgga cggctggctt
     5101 ttgccacttg ttccaaaaca ggcagcaagt cgcggatgtt gctgattttt ttgtcgaaca
     5161 acaatacaaa cggattgtcc aaagcagcga tttgtttttc agcgtcgttg atgaagtaag
     5221 gagacaagta gccgcggtcg aactgcatac cttcaaccac gtccagctcg ttttccaaag
     5281 atttgccgtc ttcaacggta atcacgcctt ctttgccgac tttttccatc gcttcggcga
     5341 taatcgcgcc gacttgttcg tcagagttgg cggaaatcga gccgacttgg gcgatttctt
     5401 tagaagtgtc gcaaggtttg gcgatgtttt tcagctcgtc aaccaaagcg gcaacggctt
     5461 tgtcgatacc gcgtttcagg tcggtcgggt tcataccggc ggtaacgtat ttcatacctt
     5521 cggcaacgat ggattgcgcc aatacggtgg cggtagtcgt accgtcgccc gccacgtcgt
     5581 tggttttgga cgcgacttct ttcaccattt gcgcgcccat attttcaaac ttgtctttca
     5641 attcgatttc tttggcgacg gttacgccgt ctttggtgat gtgcgggccg ccgaatgcgc
     5701 ggtcaaccac cacgttgcgg cctttgggac ccaaggttac gcgtacggcg tttgccagaa
     5761 tgttcacgcc gtttaccatt ttttgacgga cttcattgcc gaactgtacg tcttttgctg
     5821 ccatttcaat tctccaaaaa tcattaaaac tgtctgataa aaccgtttat gccgtctgaa
     5881 ggcggtttgc cgtttcagac ggcatcgtgt ccgtatttat ttttcaacga tgccgaaaat
     5941 atcttcttcg cgcattacca acagctcttc gccgtcggct tttacggttt ggccgctgta
     6001 tttgccgaag atgattttgt cgccgacttt gacatccagc ggacggcgcg cgccgtcttt
     6061 accgattttg cccgcgccca cggcgatgac ttcgcccatg tcgggttttt cggcggccgc
     6121 acccggcaaa acgatgcccg atgcggtttt ttcttcagct tccaagcgtt tgacgacaac
     6181 gcggtcgtgt aaaggacgga tggtcatatt tatgctccga taaaatgatt tgaaaacaat
     6241 catctgcccg aacggttcgg acagattgaa gtggaaaccg ggctgccgcc tgccggcccg
     6301 cccgtataag tcggcaaaat tagggtgtgt gcgggcaaat tcaagtgagg cggaaaaaat
     6361 ttatttccgc cgttttttat agtggactaa atttaagggg ctgtactaga ttagcagata
     6421 tgttaccctc gaaatatgaa gataacgcac tgcaaattaa agaaaaaagt acagaaagaa
     6481 ctgctccgtt tttttgtact cgaagttacc gcccgttctg ccgccgatat tttgggtatc
     6541 catcccaatt cggcagcact gttctaccgt aaaatccgca cggttatcaa ccatcatttg
     6601 gccctggctg ccgatgaggt ttttgaggat cctgtcgagc cggacgaaag cgatttcggc
     6661 ggacggcgta aaggcagacg tggtcgcggt gcggcaggaa aagtggttgt cttcggcatt
     6721 ctgaaacgca acggacgggt ctataccgtt gtggtggata atgccaagtc tgaaacgtta
     6781 ctccctgtca tcaaaaagaa aatcatgccg gacagtattg tttataccga tagtctgagc
     6841 agctgcgaca agttggacgt gagcggtttt atccattacc gcatcaacca ttccaaggaa
     6901 tttgcagacc gtcagaacca cattaacggc attgagaatt tttggaatca ggcaaaacgc
     6961 gtcttgcgaa aatacaacgg aatcgattgc aaatctttcc cgctgttctt gaaagaatgc
     7021 gaatttcgat ttaacttcgg cacaccgtct caacagctta aaatcctgcg ggattggtgt
     7081 gggatttagg gctaatctag tacagcccct aaaatttttc gttttcaagc cttcaccgct
     7141 tgccatcagc gttaaatttt tttacgataa gcacatagat tgtaaacaat cggccacaag
     7201 ccggtttgtt ttttcagaag acattatccc tgtcagacgc tgtttctata tatttcgcct
     7261 ataatggctt gtttttaata aataattcaa gaggtatcaa cgtgtctgat tccaagacga
     7321 aagaacgcgc cacattcggc acgcgccgcg cgtttatgat tgccgccatc gggtccgccg
     7381 tcggcttggg caatatttgg cgtttcccct atattgcttt tgaaaacggc ggcggcgcgt
     7441 tcatcctgcc ctatctggtc gcgcttctga cggcgggcat cccgctgctg ctgctcgatt
     7501 atgccatcgg ccaccgttac cgtggttctg cgcccttggc tttccgccgc ctcggacgat
     7561 ggtttgagcc ggtcggctgg tggaacgtga tgaccaatat cgtcatctgc atctattacg
     7621 cggtaattat cggttgggcg gcaagctata cctattattc ggtcaacgcc gcctggggtg
     7681 cggatccgca gggttttttc tttaaggact tcctgcaaat ggcgggcccg gaagccttgg
     7741 gtttggattt tgtcggcaaa gtcgccggtc ctttggcggg cgtgtgggtt tttaccgccg
     7801 ccattatggc gttgggcgtg caaaagggcg tggcgcgcgc ctcgtcgttc tttatgccgc
     7861 tgcttttggt gatgtttttg attatggtcg gcatttcact aaccctgccg ggtgcggcaa
     7921 agggcttgga cgcattgttt acgcccgact ggtcgaaact cgccgattcc aaggtctggg
     7981 tggcggcata cgggcagatt ttcttttcgc tttccatctg cttcggcatt atggttacct
     8041 attcttctta tttgaagaaa aaaaccgact tgggcggaac ggggctggtg gtcggttttg
     8101 ccaacagcag ctttgaactg ctcgcgggca tcggcgtgtt tgccgcattg ggctttatgg
     8161 cgcaggcggg cggtaaggcg gtcaacgagg ttgcctcagg cggcatcggt ttggcgttta
     8221 tcgcctttcc gaccattatc aaccaggcac cgatgggctg gctgatcggc atattgtttt
     8281 tcggttcgct ggtgttcgcc ggcgttacgt cgatgatttc catccttgaa gtgattgtgg
     8341 cggcgattca ggacaagctg aacatcgggc gcgtcaacgc cacgctgctg gtctgcattc
     8401 cgatgggcat tgtttccacg ctgctgttcg gtacggcgac ggggctgccg gttttggacg
     8461 tgatggacaa attcgtcaac acctacggca ttgttgccgc cggctttgtt tatgttgccg
     8521 ccatcatcat cagcggcagg ctgccggaat tacgcaagca cctgaacgct ttgtcctcca
     8581 tccgcatcgg cggcttgtgg acggtctgcg tcgtggttac cgtcgtgatg ctcggctata
     8641 tgctgtttaa agataccagc ggcctgatgg agaaaaatta cgaaggttat ccggatggtt
     8701 tcctcagtat tttcggctgg gggatgtcgg cggcgttggt cgtgttcggg ctgctgctgt
     8761 cgttgctgcc ttggaaacac ggtcaggatt tcaacgtcaa agacgaacac gaacatgaac
     8821 aaggagaaga aaaatgagta cttccgccat tgtgatgatg attgtctcaa tcgtgataat
     8881 ctggggaggg ctgctgcttt ccctgttaag gctgccgaac gagtaagcct ttagagcgtt
     8941 aaaaatgccg tatgaaccgc ttcagacggc attttgatgg tcggcttaca ggcaggcgag
     9001 ttcgtttgcc atttgctgtt ccaaggtttc gcgccggcgg atgagtcggt attcgttgcc
     9061 gtccaccaac acctctgccg cacggttgcg cgcgttgtaa ttgctcgcca tactggctcc
     9121 gtatgcgccc gcgctgcgga taagcagcaa atcgccttct tcgcaggcga tggtgcggtc
     9181 tttgccgagg aagtcgccgg tttcacaaat cgggccgacg atgttggcgg tcagcggcgc
     9241 gatgtttttt ggttctaccg cttcgatgtg gtggtaggca tcgtatagtg cggggcgcat
     9301 caaatcgttc atcgccgcat cgaccatcac aaagtttttc tcttcgccgt gtttgacgaa
     9361 ttcgacacgt gtcaataacg cgcctgcgtt gccgaccaag ctgcggccgg gctcaaggat
     9421 gagtttcaga cggcgtgtcc ccatcagttt ttgaaccgcc cgggcatacg cgcccaagtc
     9481 gggcacattt tcgtcttggt aaacgatgcc gacgccgccg cccaagtcca aatgttccaa
     9541 aacaatgcct tcggcggcaa gagcgtcaac caaaatcaaa atgcgttcgc aggcttcgat
     9601 gagcgggctt aagtcggtca gttgcgagcc gatgtggcag tcgatgccga tgattttcaa
     9661 attgggctgt tgtgcggcat ggcggtaggc ttcgagcgcg tcggcgtagg cgatgccgaa
     9721 tttgttggct ttcagtcctg tggagatgta gggatgggtt ttcgcatcga catcggggtt
     9781 gacgcgcagg gagacgggcg cggttttacc caaacgcgcg gcaacttttt gaatgcggtc
     9841 gatttcgggg atgctttcca tattgaagca ttttacgccg gcattcagcg cgaacttgat
     9901 ttccgcctcg cttttgccta cgcctgaaaa tatggttttt gctgcatcgc cgcctgccgc
     9961 caaaacgcgt gccaattcgc cgccggacac aatgtcaaaa ccgctgccca gcgaggcgaa
    10021 gtgtttgatg atgctcagat tgccattggc tttgacggca tagcagacga gtggggaaag
    10081 cgcggcaaag gcggtttggt agtgttcaaa cgcttcggtc agcgcggatt ggctgtacac
    10141 ataaagcggc gtgccgagtg cttcggcaag gcgggggtag gggacttgtt cgcaaaacag
    10201 ggtcatattt tcgttttcat ttttgggttg gtggggcgga ttgcggtgtg ctttgaagct
    10261 gcaaaccggt ttggattacg ccgaaacgcg ccttgtcgcc ttctttgggc aggtagaggt
    10321 cgcctttgta accgcaggcg gaaagcagga gggcggttgc cgccgcaaaa aatacgccgt
    10381 atttcatcgg taaacttcct tcataagcgc gaatgtggca agattcggca tcttaaacaa
    10441 aaaacacgca aaaagccatg atgaccgaaa gcgagtttat ccgcgcgagc gaagcattat
    10501 ttgaacacat cgaagaccaa atcgacgaaa acggctggga tttcgactgc cggtttgccg
    10561 gaaacgtcct gaccatcgaa gccgaaaacg gcacgcaaat catcgtcaac cgccacacgc
    10621 ccaaccaaga attatggatt gccgccaaaa gcggcggcta ccatttcgcc gaacaaaacg
    10681 gcaaatggct ggcaacgcgc gacaaccgcg atttttacga cgttttaaac gaagccctga
    10741 gcgcggcttc gggcgaagcg gtaaagattg ccgaattgta atttgagtgt tatcacggag
    10801 agaaaaaatg aacacacgtc ccttttattt cggacttata tttatcgcga ttatcgctat
    10861 atttgctaac tatttaggaa acactgattt ttcccatcat tatcatatca gtgctttaat
    10921 tattgctatc ttgctgggaa tggcaatcgg caataccatt tatccgcaat tttcaacaca
    10981 agtggaaaaa ggcgttttgt ttgccaaagg cacgcttctt cgcactggca ttgtgctgta
    11041 tggtttccgc ctcacttttg gcgatattgc cgatgtagga ttaaatgcgg ttgtcactga
    11101 tgcaatcatg ctaatttcaa ccttcttttt taccgtactt ttaggcattc gttatctaaa
    11161 aatggataaa caattggttt atctcactgg ggcaggttgc agcatttgcg gtgcggcagc
    11221 ggttatggcg gcagagtctg ttaccaaagc agaatcccat aaagtttcag tggcgattgc
    11281 catagtggtc attttcggga cgcttgctat ttttacttac cccttgttct acacgtggtc
    11341 acaagattta attaacgccc atcaattcgg tatttatgtt ggttctagtg tacacgaagt
    11401 ggctcaagtg tatgcgattg gggaaaatat tgatcctatc gtggcgaata ctgccgtcat
    11461 ttccaaaatg atccgagtga tgatgctcgc ccccttttta ttaatgcttt cttggttatt
    11521 aacacgtagt gatggggtat cagaaaatac ctcacacaaa attacaattc cttggtttgc
    11581 tgtacttttt attggtgttg ccatttttaa ttcttttgat ttattaccaa aagaactcgt
    11641 gaaattattc gttgaaatcg attctttctt attaatttca tcaatggctg cgcttggctt
    11701 aacgacgcac gcaagcgcaa tcaaaaaggc aggattaaaa ccgtttgttt taggaatact
    11761 aacttattta tggctagtgg ttggtggatt tttagtgaac tatggaatat caaaattaat
    11821 ataaaattca ctaaagagag cgttacccaa tggcacaatt accgctatat ctgacttctg
    11881 aaatcaaaga ctttactgtc ggcacgccta aagttttgga atcattttcc aaacatatcc
    11941 cttatggtgt tgtctttgaa gacgacggcg acacgggcta cttctatgcc gcttcgcaag
    12001 acgggattct agatgccttg cacatctaca atgtcgaaga tgtatccgac aaacatatcc
    12061 ccaatcatgt cttgatttta tgggatgatg cctgcaccat agccgcattg agtatcaacg
    12121 actacattca tgccgtctat gattttgtcg aacaggcagg atattgccgc aacggcttcc
    12181 ctgaaacagg cggcgaatgg gtgaaagtcg aaaaccgcgt cttggacgat gaattgctgg
    12241 acaaaatctt atcccgaaaa tctacataac ctactcaaaa ggataacaaa atgcccctgt
    12301 tagacagttt caaagtcgac cacacccgta tgcacgcccc tgccgtacgc gtggcgaaaa
    12361 ccatgaccac gcccaaaggc gataccatta ccgtattcga cctgcgcttt tgcgttccta
    12421 acaaagaaat cctgcctgaa aaaggcatac acacgctgga gcatttgttc gcaggcttta
    12481 tgcgcgacca cttaaacggc aacggcgtcg aaatcatcga catttccccg atgggctgcc
    12541 gcaccggttt ttatatgagc cttatcggca cgccttccga acagcaggtc gccgatgcgt
    12601 ggctggcttc gatgcaggat gttttgaatg tcaaagacca aagcaaaatc cccgagttga
    12661 acgaatacca atgcggcact tatcaaatgc actcgctcgc cgaagcgcaa caaatcgcgc
    12721 aaaacgtgtt ggcgcgcaaa gtggcggtga acaaaaatga agagctgacg ctggatgaag
    12781 ggctgctgaa cgcctaatcc gccaaaaatg ccgtctgaac aagggtttca gacggcattt
    12841 gccttttccg ttataatctg gggttgtgcg ggggcgggtt ttaagccggc atcgtccttc
    12901 cctatttttt tctgtccctt atcggtttta agcgggtttt ttatgtccaa cagacctaca
    12961 ctcctcctcg ttgacggatc gtcctacctc taccgtgcgt atcacgcgat ggggcaaaac
    13021 ctgaccgcac ccgacggtgc accgacgggt gcgctgtatg gcgtattgaa tatgttgcgc
    13081 cgtttgcggt cggaatatcc gcacgattat tgcgcggtgg tttttgatgc gaaaggcaaa
    13141 aatttccgcc atcaaatgtt tgaagaatac aaggcgacgc gcccgccgat gcccgacgat
    13201 ttgcgtccgc aggcggaagc tttgccggat ttggtgcgcc tgacgggctg gccggtattg
    13261 gtgattgggc aggtggaagc ggacgatgtg atcggcacgc tggcaaaaca gggggcggaa
    13321 catggtttgc gagtcattgt ttcgaccggc gataaggaca tggcgcagtt ggtggatgag
    13381 cgcgttacgc tggtgaacac gatgagcgga gaaacgctgg atattgaagg cgtgaaggca
    13441 aaattcggcg tgcgccccga ccaaatccgc gattatctcg cgctgatggg cgacaaggtg
    13501 gacaacgtgc cgggcgtgga aaaatgtggc ccgaaaacgg cagtgaagtg gctggaagcc
    13561 tacggctcgt tgcaaggcgt gatagaacac gcttcggaaa tcaagggcaa agtgggcgag
    13621 aatctgcgtg ccgccctgcc gcgcctgccg ctgtcgtatg atttggtaac gattaaaacc
    13681 gatgtggact tgcacgccga gctttcagac ggcctcgaaa gcctgcgccg taccgcgccg
    13741 aaatgggcgc agctggttgt cgatttcaaa cgctggggct tccgcacctg gctgaaggaa
    13801 gcggaaagcc gtatgcacga agcggcggac ggcgatttgt tcggcagcga tacgataggc
    13861 gaacaggcgg cgttggatat ggaaacgtcg tctgaaaggc tacctgaaaa agccgttgcc
    13921 cccgaaaagc tggattatca ggccgttacc accgaagcgc agtttgccac tttgttagac
    13981 aaattgtcgc aggcggaaaa aatcggcatc gacacggaaa ccacttccct agacgcgatg
    14041 aacgccgcgc tggtcggcat cagcatcgcg ttccaagcgg gcgaagcagt ttacatcccc
    14101 gtaggacaca gcctgaccgc cgcgccagaa cagcttgatt tgcaagacgt attaggccat
    14161 ctgaaaccgt atttggaaaa tcctgccctg aaaaaaatcg ggcaaaacct caaatacgac
    14221 caacacgttt tcgccaatta cggcatcgcc ctgaacggca tcgccggcga cgccatgctc
    14281 gcttcctaca tcatcgagag ccatctcgga cacggcttgg acgaattgtc cgaacgctgg
    14341 ctcggcttgg aaaccattac ctacgaatcg ctgtgcggca aaggcgcgaa gcaaatcggt
    14401 tttgccgatg tcgccatcgg gcaggcgacc gaatacgccg cccaagacgc cgatttcgcc
    14461 ctgcgcctcg aagcgcacct gcgcgcgcaa atggacgaaa aacagcttga aatgtatgaa
    14521 aaaatggagc tgcccgtcgc gcaggtattg tttgaaatgg aacgcaacgg tgtgcaaatc
    14581 gaccgcgccg aactcgcccg ccaaagcgcg gaactcggcg ccgagctgat gaagctcgaa
    14641 caagaagcct acgccgccgc agggcagccg ttcaacctca actcgcccaa acagctgcaa
    14701 gaaatcctgt tcgacaaaat gggcatcccg acaaaagggc tgaaaaaaac cgccaagggc
    14761 ggcatttcca ccaacgaggc cgtgctcgaa cagctcgcgc ccgactaccc cttgcccaaa
    14821 atcatcctgc aaaaccgcag cctggcgaaa ctcaaatcca cctacaccga caaactgccc
    14881 gacatgattt ctccccggga taaccgcgtg cataccacct acgcccaagc cgtcgccatt
    14941 acaggacgct tggcaagcaa caaccccaac ctgcaaaaca tccccatccg caccgccgaa
    15001 ggccgccgcg tgcgccgcgc ctttaccgcg ccaccgggca gcgtcatcgt ttccgccgac
    15061 tattcccaaa tcgaactgcg catcatggcg cacctctctg gagacaaaac cctcattgcc
    15121 gcattccaaa acggcgaaga cgtacaccgc cgcaccgccg ccgaagtgtt cggcactgcg
    15181 cccgaaaacg tctcgcctga gcaacgccgc tacgccaaaa ccatcaactt cggcttaatc
    15241 tacggtatgg ggcaatacgg ccttgccaaa tcattgggca tcgacaacct ttccgccaaa
    15301 aactttatcg accgctactt cgcccgctac cccggcgtcg ccgaatacat gcagcgcacc
    15361 aaagaacaag ccgccgccca aggcttcgtc gaaaccctgt ttggccgccg cctctacctg
    15421 cccgacatcc gcaacaaaaa cgccaacgcc cgcgccggag ccgaacgcgc cgccatcaac
    15481 gcccccatgc aaggcaccgc ctccgacctt atcaaacgcg ccatgataga cgtgtcccgc
    15541 tggctggttt cagacgacct gaagagcaaa ctgattatgc aggtgcatga cgaactggtg
    15601 ctggaagtcg ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg
    15661 aaagtgggcg gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat
    15721 tgggaagagg cacattgatt gaaaggtgtt attatgctat ctttatttaa ataaaattta
    15781 atttttggta tattttttct aaatgttcct a
//
LOCUS       DAWFIT010000042        15718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFIT010000042 DAWFIT010000000
VERSION     DAWFIT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.042"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            101..220
                     /locus_tag="WGQ85_001222"
     CDS             101..220
                     /locus_tag="WGQ85_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0750653.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            233..1096
                     /locus_tag="WGQ85_001223"
     CDS             233..1096
                     /locus_tag="WGQ85_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0750654.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGQ85_001224"
     CDS             complement(1147..2199)
                     /gene="sohB"
                     /locus_tag="WGQ85_001224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0750655.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2303..2392)
                     /locus_tag="WGQ85_001225"
     tRNA            complement(2303..2392)
                     /locus_tag="WGQ85_001225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2356..2358),aa:Leu,seq:taa)
     gene            complement(2495..2568)
                     /locus_tag="WGQ85_001226"
     tRNA            complement(2495..2568)
                     /locus_tag="WGQ85_001226"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Cys,seq:gca)
     gene            complement(2594..2669)
                     /locus_tag="WGQ85_001227"
     tRNA            complement(2594..2669)
                     /locus_tag="WGQ85_001227"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Gly,seq:gcc)
     gene            complement(2691..2766)
                     /locus_tag="WGQ85_001228"
     tRNA            complement(2691..2766)
                     /locus_tag="WGQ85_001228"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2731..2733),aa:Gly,seq:gcc)
     gene            complement(2790..2865)
                     /locus_tag="WGQ85_001229"
     tRNA            complement(2790..2865)
                     /locus_tag="WGQ85_001229"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2830..2832),aa:Gly,seq:gcc)
     gene            complement(2891..2966)
                     /locus_tag="WGQ85_001230"
     tRNA            complement(2891..2966)
                     /locus_tag="WGQ85_001230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2931..2933),aa:Gly,seq:gcc)
     gene            complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGQ85_001231"
     CDS             complement(3046..3609)
                     /gene="pgsA"
                     /locus_tag="WGQ85_001231"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0750656.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3715..4138)
                     /locus_tag="WGQ85_001232"
                     /pseudo
     CDS             complement(3715..4138)
                     /locus_tag="WGQ85_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4262..4597)
                     /locus_tag="WGQ85_001233"
     CDS             complement(4262..4597)
                     /locus_tag="WGQ85_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0750657.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            4726..5631
                     /gene="mtrA"
                     /locus_tag="WGQ85_001234"
     CDS             4726..5631
                     /gene="mtrA"
                     /locus_tag="WGQ85_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0750658.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(5693..6181)
                     /locus_tag="WGQ85_001235"
     CDS             complement(5693..6181)
                     /locus_tag="WGQ85_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0750659.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6328..7230
                     /locus_tag="WGQ85_001236"
     CDS             6328..7230
                     /locus_tag="WGQ85_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0750660.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7271..8401)
                     /locus_tag="WGQ85_001237"
     CDS             complement(7271..8401)
                     /locus_tag="WGQ85_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750661.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKVG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPSHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            8606..11230
                     /gene="alaS"
                     /locus_tag="WGQ85_001238"
     CDS             8606..11230
                     /gene="alaS"
                     /locus_tag="WGQ85_001238"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0750662.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            11292..12023
                     /locus_tag="WGQ85_001239"
                     /pseudo
     CDS             11292..12023
                     /locus_tag="WGQ85_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12732..15143
                     /locus_tag="WGQ85_001240"
     CDS             12732..15143
                     /locus_tag="WGQ85_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0750663.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAHRTFQWTSQAAGKAAVH
                     CIIVGFRQKPPMPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPIHCE
                     PDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLWFHG
                     VSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGNYLI
                     IPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAGRLK
                     SDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQEAG
                     LPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFELYRK
                     AAAIA"
BASE COUNT         3999 a         4265 c         3730 g         3724 t
ORIGIN      
        1 ggaggattag gggggtattg gggtaaaatt agtggatatt tgaaacgaaa acagccgaaa
       61 acctgtgttt gggtgtcagc tgtcgggagg gaaaggaatt ttgcaaaggt ctcacaaagt
      121 atttgccaca ttcggcgaca agcgcacaac ccggctgttt gccgatttgg atgcactggc
      181 tgaagtgatg gaaaaaacaa tctcggaaaa taaaaaatag gggggcaaat atgtggaaaa
      241 tgttgaaaca catagccaaa accaccgcaa gcgattgatt ggcacatttt ttctggtcgg
      301 actggaaaac cttttgatgc tggtgtatcc ggtgtttggc ggctgggcga ttaatgccgt
      361 gattgcgggg caggcgtggc aggcgttgct gtacgctttg gttgtgcttt tgatgtggct
      421 ggtcggtgcg gcgcggcgga ttgccgatac gcgcacgttt acgcggattt ataccgaaat
      481 cgccgtgccg gttgtgttgg aacagcggca gcggcaagtc ccgcattcgg cggtaactgc
      541 gcgggttgcc ctgtcgcgtg agtttgtcag cttttttgaa gaacacctgc cgattgccgc
      601 gacatccgtc gtatccatat tcggcgcgtg catcatgctg ctggtgctgg aattttgggt
      661 cggcgtgtcg gcggtgggca tacttgcgtt gtttttatgg cttttgccac gttttgccgc
      721 catcagcgaa aacctgtatt tccgcctgaa gaacagcttg gaacgcgaca accactttat
      781 ccgaaaaggc gacgagcggc agctggaccg ccattacgga ctgcttgcgc gcctgcgtgt
      841 gctgatttcc aaccgcgaag ccttcggcta tctctgcgtc ggcacggcga tgggtatttt
      901 gttcggcttt gcttttgtga tgatgacgct caaaggctac agcagcgcgg ggcatgtcta
      961 ttcggtcggc acttatctgt ggatgtttgc catgagtttg gacgacgtgc cgcgattggt
     1021 cgaacaatat tccaatttga aagacatcgg acaacggata gagtggtcga aacggaacat
     1081 caaagccgga acttgaaaaa tgccgtctga acacgcttca gacggcattt ccatccgttc
     1141 ggcaaactac atcacatccg cccgccggtt gacaagtttg gcaaacaact tttcaacaga
     1201 agcttccgcc tgcaaaccaa tgcgctggat caggctttgc ttctcctgat atttcacttc
     1261 gataacctgt ttgttttcaa acgctttcaa caacaaatca tcactggtcg aaatctcgtc
     1321 aatcaagttc aacgccaacg cctgccgacc gaaccaatgc tcgcccgttg ccacttcctc
     1381 aatatccaat tgagggcggt tctcgctgac aaactgcttg aacaactgat gcgtttcctc
     1441 cagttcctgt cggaatttct gtttgccctt ttccgtattt tcacccataa aagtaaccgt
     1501 gcgcttaaat tcgcccgccg tcatcacatc cacatcaata tcatgttttt tcaacaggcg
     1561 gtggatattc ggtacttccg ctacaacacc caccgaaccg acaatcgcaa acggagcgga
     1621 aacaatttta tccgccacac acgccatcat ataaccaccg ctcgccgcca ccttatcgac
     1681 ggcgacggtc agcggaatat tgcgttcgcg caaacgccta agctgcgaag ccgccaaacc
     1741 gtaaccgtga accacgccgc ccggactttc caatctaagc agaacctcat cttcaggctt
     1801 ggcaatcaaa agcaccgccg taatctcatg acgcaaggat tctacggcgt gtgcatacaa
     1861 atcgccgtca aaatccaaca caaaaaggcg ggatttttgc gtttcggaag atttctcccc
     1921 accctccttc aaacgctttt tctctgcttt ggcttccgcc ttttcctttt tcttttcctc
     1981 tttttcctga tgttttgcct cttccccgct taaaaagaat gcttcaaacg attgccgctg
     2041 ttttttataa ttttccgaaa aatccgtcag tacgacactg ccgctttccg actgtttctt
     2101 actctgtacg atagccaaca caatcagcgc aattgcgccg aacacggtaa gcagttcgag
     2161 caggaaaata ccgtaattca gtaaaatttc tttccacatt gattggattt cctcttgttc
     2221 aggcatgaac atgtcaatat tgtccatcac cgtccgacag ataaaaaaat aaccgcttgg
     2281 agcggcattg tcattttcag cttggtgccc ggagccggaa tcgaaccggc acgggatgtt
     2341 tagtcccgac ggattttaag tccgttgtgt ctacctattt caccacccgg gcatttgtga
     2401 aaggtggagg cgggggcgcg gattttaacc ggcctatatg aagattgcac tcctcatagc
     2461 ataaacactc tgccaccccg ccgtagtacg ataatggagg cgagagtcgg aatcgaaccg
     2521 gcgtagacgg atttgcaatc cgctgcataa ccactttgct atctcgccct aaaactggct
     2581 tatctaaaaa acttggagcg ggaaacgagt ctcgaactcg cgacctcaac cttggcaagg
     2641 ttgcgctcta ccaactgagc tattcccgcg cgttcaaaca tatcggtttt tggagcggaa
     2701 aacgagtctc gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctat
     2761 tcccgcgttg atatgtttga aataaaactt ggagcgggaa acgagtctcg aactcgcgac
     2821 ctcaaccttg gcaaggttgc gctctaccaa ctgagctatt cccgcaatga ttgtgggaaa
     2881 atgaaatttt tggagcggga aacgagtctc gaactcgcga cctcaacctt ggcaaggttg
     2941 cgctctacca actgagctat tcccgcccga tttcattctc caacatcgaa gagacgcaat
     3001 tattatggat tctgtttttg ccgtcaagct atttttatgt ttttttcagg cgatttcttt
     3061 ccacgccatt ttcagataat acagcatcga ccagactgtc agcaaagatg cgataaacat
     3121 caatacattg ccgataacaa caagattaaa tccgtaaaaa tcgggaatat tcagcagcag
     3181 caggaaaatt gccagcattt gcgcggcggt tttgaactta ccgacggtgg cgacggcaac
     3241 gctgttcctt ttgcccattt gcgccatcca ttcgcgcaat gcggaaatgg taatttccct
     3301 gccgataatg atcatggcaa acaaaacata ggtccggtcg agtttgacca gtaaaagtag
     3361 cgatacggca accatcagct tgtcggcgac gggatcgagg aaggcgccga aatcggaagt
     3421 ctgtttccac agtcttgcca aaaatccgtc aaaccagtcg gttaaggcgg caacggcaaa
     3481 aatgacggcg gcggtgagat taatcgtttc ctccgcgaac cacggaaaag gcaggtaaaa
     3541 aagggctgtc atgacaggaa tgagcaagac cctcaaccat gtgaggaaga tagggagatt
     3601 ccaaggcatc ggttttctct gtgcagactg taaagttgtg attataacgg ttatcctcat
     3661 aacccaaaac gtaaaattgc tgcatgggca ttcccccgcc ccgccaatct gttttcacat
     3721 tcttttcaaa cgcaggaaaa tggcgggcaa taaaagcaaa atacccagtt tcaggcagaa
     3781 aacggcaggt tgtgccaaca cttcgacaag gcggtcttcc gtgcgggcaa aatctttatt
     3841 gcttatagac actgccactg ttgcggtatt ccaacagaac gccgtttaaa aaacctttgc
     3901 cgacggtttc gcttaaaacg gctctaacct gctccgccct gatggttctg ccgatattgc
     3961 cgcctgtgca caaactgtcg aacccatagc aggaaagccg gtaatgctgc ccgtctgcat
     4021 ccagtttgat tgcccgtccg ctgcggttgg gggcggtaac ggtcaattcc gcatattcga
     4081 atgttttttc ttgttcgtga aatgccgtca ggtaaggtgc aataaaaacg gcggacaaca
     4141 gcagacagct tatggcggca aaccataccc agcgataata tattgtttac agtattggga
     4201 agcataacag acaaaatgcc gtctgaaata ttttcagacg gcatttctta tccgaaacgg
     4261 attatttttg cgtttcaacc gcttccaatg cacgcagggc gtaagtgtaa gcggcaccgg
     4321 cattcagggc gatggcggtt gccaatgcgc ctgcgatttc gctgtcggtc gcaccggctt
     4381 tggcggcagc ggcggcgtga acgctgatgc agctctcgca acgagtggta atggcaacgg
     4441 cgatggcaat cagttcgcgc gttttggcat caagtgcttc ggcggcggcg gcctgttcca
     4501 atgcgccgta ggcctgcagc attttaggat gcgccttacc cagctcgccg aacgattttt
     4561 taaccaatgc ggtatgttct ttccaatctt taaacatttt cttttccttt ctcttgcgtt
     4621 taaccctgat acgcgcttgc gtatctgttt tcgatgtgcg tattattgca attattcagt
     4681 tgtgtttctc gtttaatcat ctcattttat ggttcaaaaa gatttatgga cattctggac
     4741 aaactggtcg atttcgccca attgacgggc agtgtggatg tgcagtgcct tttgggcgga
     4801 caatggtcgg tacggcatga aaccttgcaa cgcgaaggat tggtacacat tgttacatcg
     4861 ggcagcggct atctctgcat cgacggcgaa acttccccgc gtccggtcag tacaggggat
     4921 attgtatttt tcccgcgcgg cttgggtcat gtgttgagcc acgacggaaa atgcggagaa
     4981 agtttacaac cggatatgcg gcagcacggt gcgtttacgg tcaagcagtg cggcaacgga
     5041 caggatatga gcctgttttg cgcccgtttc cgctacgaca cccacgccga tttgatgaac
     5101 gggctgcctg aaaccgtttt tctgaacatt gcccatccga gtttacagta tgtggtttca
     5161 atgctgcaac tggaaagcaa aaaacctttg acggggacgg tttccatggt caacgcattg
     5221 tcgtccgtcc tgctggtgct tatcctgcgc gcctatctcg aacaggataa ggatgtcgaa
     5281 ctctcgggcg tattgaaagg ttggcaggac aaacgtttgg gacatttaat ccaaaaggtg
     5341 atagacaaac cggaagacga atggaatgtc gacaaaatgg tggcggctgc caatatgtcg
     5401 cgcgcgcaac tgatgcgccg tttcaaaagc cgggtcggac tcagcccgca cgcctttgtg
     5461 aaccatatcc gcctgcaaaa aggcgcgttg ctgctgaaaa aaaacccgga ttcggttttg
     5521 tcggtcgcac tgtcggtagg ctttcagtcg gaaacgcact tcggcaaggc gttcaaacgg
     5581 caatatcacg tttcgccggg tcaataccgg aaagaaggcg ggcaaaaata aatcggggct
     5641 tcaaacgcaa atgccgtctg aaaaggcttt catacagcat ttgcgtaccg cgtcatttca
     5701 agggctgcat cttcatcact tccatcaaaa agttggtaaa tgcggggttg ttgggtttga
     5761 catccatatt tttccaacgc tgctgccagc cgcgcaaggc attctggata tacagcttgg
     5821 actgttccgt attgattgcg ccccgctggc tgtctatcgc cgaacgcagg tagatttcat
     5881 acatactgtc atcgacggca ttgcgtccga ccaggcgttt tctgaagttg ttcagatatt
     5941 gcgccgcctg aaccttggtc attttaccga tacccacctg atagcccaag cgcgtcgctt
     6001 catcgctgat tttggcaaca tccgtccaat gcgaagaggc aaggcggaaa ccttttgcag
     6061 gtgcttccgt tttgacggta ttgataggat tcacggggat ttccgtcaat gtgggcacat
     6121 aaatagactg gcagccggaa agaactgcca caatggaaag agggataagg tattttttca
     6181 tgcccccatt ataatcaagt ttgccttgag aaaacaaatt gttcggcaag aaaaataaaa
     6241 tttcggcatc agaagcaggc aaaaacacat tccacaagcc ttgccgcaag gtttacaatc
     6301 cgaccgtcct tatcgcaacg accgtttatg gataccgcaa aaaaagacat tttaggatcg
     6361 ggctggatgc tggtggcggc ggcctgcttt accattatga acgtattgat taaagaggca
     6421 tcggcaaaat ttgccctcgg cagcggcgaa ttggtctttt ggcgcatgct gttttcaacc
     6481 gttgcgctcg gggctgccgc cgtattgcgt cgggacacct tccgcacgcc ccattggaaa
     6541 aaccacttaa accgcagtat ggtcgggacg ggggcgatgc tgctgctgtt ttacgcggta
     6601 acgcatctgc ctttggccac cggcgttacc ctgagttaca cctcgtcgat ttttttggcg
     6661 gtattttcct tcctgatttt gaaagaacgg atttccgttt acacgcaggc ggtgctgctc
     6721 cttggttttg ccggcgtggt attgctgctt aatccctcgt tccgcagcgg tcaggaaacg
     6781 gcggcactcg ccgggctggc gggcggcgcg atgtccggct gggcgtattt gaaagtgcgc
     6841 gaactgtctt tggcgggcga accctgctgg cgcgtcgtgt tttacctttc cgtgacaggt
     6901 gtggcgatgt cgtcggtttg ggcgacgctg accggctggc acaccctgtc ctttccatcg
     6961 gcagtttatc tgtcatgcat cggcgtgtcc gcgctgattg cccaactgtc gatgacgcgc
     7021 gcctacaaag tcggcgacaa attcacggtt gcctcgcttt cctatatgac cgtcgttttt
     7081 tccgccctgt ctgccgcatt ttttctgggc gaagagcttt tctggcagga aatactcggt
     7141 atgtgcatca tcatcctcag cggcattttg agcagcatcc gccccactgc cttcaaacag
     7201 cggctgcaat ccctgttccg ccaaagataa aaaatgccgt ccgaacatcc ttcagacggc
     7261 atatcgggct ttatttcccc gccttcacat cctgccactg gcgcaccata aacttcaatg
     7321 ccgccggctg gataggcacc atgataaagc tgtttttcaa atcctcctcg gttgggaaaa
     7381 tcgtattgtc gtttttaaat tcgtcttcca tcagctcacg cgccggcttg ctcgaaggcg
     7441 cataagtaac gaaattgccg tttttcgccg acacttccgg gtcgaggaag tcgttgatgt
     7501 atttgtgcgc gttggcgacg tttttcgcat ctttcggaat cacgaaagaa tccacccaaa
     7561 tccccacgcc ctctttgggc atcatcacgc ggattttttc cttgccgccc gcttcttcgg
     7621 cacggcgttt ggcgatgttc aaatcgccgc cgaaaccgat tgttacgcag gtatcgccgc
     7681 gcgccaaatc atcgataaag ccggacgaag taaagcgttt gatattgggg cggtttttct
     7741 tgagcagggc ggttgcctcc ctgatgtctt ccgtattgct gctgttcggg tttttaccca
     7801 aatagttcaa caccatagga tagatttccg ccgcgctgtc taaataactg ataccgcatt
     7861 gtttgagttt ggacgtgtat tcggggtcga acaccaaatc ccactggttg tccggcagct
     7921 tgtccgtacc caaagccttt ttcacgcgtt cggtattgat ggcgaaggta tttgtccccc
     7981 aataaaacgg cacggcgtat tcgtggctgg gatcgacccc gtccatcagc ctcatcattt
     8041 cgggattgag gtgtttataa ttgggaatca gcgatttatc gattttctga tacgcaccta
     8101 ccttaatctg cctgcccaca aacgcattgg acggcgcgac aatgtcgtaa ccggattttc
     8161 cggtcagcac cttgctttcc agcgtttcat cgctgtcgta cacatcataa gtaaccttga
     8221 tgccgttttt cttttcaaaa tcggcaacgg tttccggatc gacgtattcc gaccagttgt
     8281 aaattttcaa tacgtttcgg ttttccgccg gcgccggttt ttcggcaggc ggtttgtccg
     8341 aaccgccgca cgctgcaagc agcaaagcag tcaggacggc gagtggcaga tgtttggcca
     8401 ttatcattcc ttgcatatcg ggttggagaa agcggtcatt atagccgata ttggcaacag
     8461 ggcttcagac ggcattcaaa atcccgccac acccttccga aaaccgccgc ttccatagct
     8521 agaaacaggg atttgcggta agataccgcc gttcgttttc cctgctttta ccatgacaag
     8581 acatttgaga gacattgaaa aaattatgaa aacctccgaa ctgcgccaaa aattcctaaa
     8641 attttttgaa accaaaggcc acaccgtcgt ccgctcttcc agcctcgtgc cgcacgacga
     8701 cccgaccctg ctgtttacca acgcgggcat gaaccagttt aaagacgtat tcttaggttt
     8761 cgacaaacgc ccgtacagcc gcgccaccac cgcgcaaaaa tgcgtacgcg caggcggcaa
     8821 gcacaacgac ttggaaaacg tcggctacac cgcccgccac cacaccttct ttgaaatgat
     8881 gggcaacttc tccttcggcg actacttcaa gcgcgacgcg atccacttcg cttgggaatt
     8941 tctgacttcc cccgaatggc tcaatatccc caaagacaaa ctgttggcga ccgtttacgc
     9001 ggaagacgac gaagcctaca acatctggtt gaacgaaatc ggtatgccgt ccgagcgcat
     9061 cgtccgcatc ggcgacaaca aaggcgcgaa atacgcgtcc gacaacttct ggcaaatggg
     9121 cgacaccggc ccttgcggcc cctgctccga aattttctac gaccacggcg aagaaatttg
     9181 gggcggcatt cccggcagcc ccgaagaaga cggcgaccgc tggatcgaaa tttggaactg
     9241 cgtattcatg cagttcaacc gcgacgaaca aggcaatatg aatccgctgc ccaagccgtc
     9301 cgtcgatacc ggcatgggct tggaacgcat ggccgccgtt atgcagcatg ttcacagcaa
     9361 ctacgaaatc gacttgttcc aagacctgct caaagccgtt gcccgcgaaa ccggcgcagc
     9421 tttcagcatg gaagaaccaa gcctaaaagt catcgccgac cacatccgct cctgctcatt
     9481 cctgattgca gacggcgtat tgccttccaa cgaaggccgc ggctacgtat tgcgccgcat
     9541 tatccgccgc gccgtgcgcc acggttacaa actgggtcaa agcaaaccgt tcttccacaa
     9601 actggtggcc gatttggtca aagagatggg cgatgcctac cctgaattga aagaaaaaca
     9661 agttcaaatc gaagaagcgt tgaaaaacga agaaagccgt tttgcccaaa ctctggaaac
     9721 cggtatggct ttgttggaaa acgcgcttgc caaaggcagc aaaaaactcg acggcgaaat
     9781 catcttcaaa ctctacgata cctacggttt cccatacgac ttgactgccg acatctgccg
     9841 cgaacgcaat atcgaactgg acgaagcagg cttcgagcgc gaaatggaag cccaacgcgc
     9901 acgcgcacgc gccgcacaaa gcttcaaagc caacgcccaa ctgccttatg acggtcaaga
     9961 caccgagttt aaaggttata gcgaacgcca aaccgaatcc aaagtcctcg ccctctacaa
    10021 agacggcgag caagtcgacg aattgaacga aggcgacagc ggcgcagtcg tcatcgactt
    10081 taccccgttc tatgcagaat ccggcggcca agtcggcgat gtcggctata tcttctcagg
    10141 cgaaaaccgc tttgaagtac gcgataccca aaaaatcaaa gcggccgtat tcggtcaatt
    10201 cggcgtacaa acttcaggcc gtctgaaagt cggcgacagc gttaccgcca aagtggacga
    10261 cgaaatccgc aatgccaata tgcgcaacca cagcgcaacc cacttgatgc acaaagccct
    10321 gcgcgatgta ttgggcagac acgtcgaaca aaaaggctct ttggttaccg ccgaatccac
    10381 ccgtttcgac atttcccatc cccaagcggt aactgccgaa gaaatcgccg aagtcgaacg
    10441 ccgcgtcaac gaagccgttt tggcgaacgt tgccgtcaac gccgccatta tgagcatgga
    10501 agacgcgcaa aaaaccggcg ccatgatgct cttcggcgaa aaatatggcg acgaagtacg
    10561 cgtattgcaa atgggcggtt tctctaccga attgtgcggc ggcacgcacg tttcacgcac
    10621 cggcgacatc ggcctcttca aaatcatcag cgaaggcggt attgccgcag gcgtgcgccg
    10681 tatcgaagcc atcaccggcc tgaacgcact caaatgggcg caagagcaag agcgtttggt
    10741 gaaagacatc attgctgaaa ccaaagccca aaccgaaaaa gacgtactgg cgaaaatcca
    10801 agcaggcgcg gcacacgcca aagcattgga aaaagaattg gcacgcgcca aagccgaact
    10861 cgccgtccac gcaggcgcaa aactcttgga cgacgcaaaa gacttgggcg cagccaaact
    10921 cgttgccgcc caaatcgaag ccgacgcagc cgccctgcgc gaaatcgtaa ccgacctgac
    10981 cggaaaatcc gacaacgccg tgattctttt agcggcagta aacgacggca aagtctccct
    11041 gtgcgccggc gtatccaaac cgttgaccgg caaagtgaaa gcaggcgatc tggttaaatt
    11101 tgcagccgaa caagtcggcg gcaaaggagg cggccgtccc gacttggcgc aggccggcgg
    11161 cacggatgcc ggcaaattgc ccgaaatgct ggtaagcgtg gaaagctggc tttgtcagaa
    11221 attaagttaa atttaaatct taataagcca tttgagtctt gttcaaatgg cttatatttt
    11281 ggattgttag aatgaatact aaaacagagt tacaaaaact tttagaagag gatatatcaa
    11341 ctttaacaga aacactaatt tgtgcagatg ctcttcctcc aagatatgta cgtagcattg
    11401 caactcctat tgtccgtcga tggttaattg acaaacaatt aaatattctc gcaaaagaaa
    11461 taggtttaac tatagaattg cccatccttg atacaagttt ggtatttgaa aaattgtcaa
    11521 ccttagagaa taaagttaat ttttatatgg ctggtggagt ttatttaggc ggagaattta
    11581 ttagctctat atatcattca tcacaagaat tttctggtga accaataatc tatgctgaac
    11641 caaatataat tttatgtcct gctgaaaaat ttttaacttt aaaacgagtt ttccataatg
    11701 gaaatatttt taatatgaat cagataatta cttttttatc caataaacaa ggaggagtac
    11761 attttgataa aaattacgat aaatataaaa cttgacaagt tgctatagaa aaagcagcaa
    11821 attttttaaa actcggtaat ccatataatg aagataaatt atctcttagt gaagaacatg
    11881 atactatttt agtagtttta ccattagaaa aaggatatga atggaattgt cttgagatag
    11941 aagtactatc cgctgctcaa tctctagcaa atatctattg caacaaagtt cgcctcatag
    12001 atggtcatgt ttggaaggaa taaaagaaaa agcatagttg gtaattttta gttacgtagg
    12061 gtgggcttca gcccaccgtt ttttcatttt tgattgttga ttggtgggct aaagcccacc
    12121 ctacggttac taaaatcccg tcattacctc gcaggtggga atccggtttg ttcggtttcg
    12181 gttttttagg gacagtgccg caacattaaa tttccagatt cccactttcg cacaaataac
    12241 agcgcaaata gggcaaagat aaggatatgg gtttttttct ttgaatttac tatacaattc
    12301 gcccgtcccc gttatagaaa cccaagccga tgaaaaccct gctccaactc caaaccgccg
    12361 cacaaaactt cgccgcctac tacaaagacc aaaccgacga acgccgcgag aaagacacct
    12421 tctagaacga atttttcgcc attttcggca tcgaccgcaa aaacgtcgcc cacttcgaat
    12481 accccgtcaa agaccctgcc gacaacaccc aattcgtcga tatattttgg gaaggcatct
    12541 tccttgccga acacaaatcc gccaacaaaa acctgaccaa ggccaaagag caggcggaac
    12601 gttatttaca ggaaatcggg cgcaccaagc cctccgcgct gcccgaatat tacgccgtca
    12661 gcgattttgc ccatttccac ctttaccgcc gcgtacctga agaaggcgca gaaaaccaat
    12721 ggcagttccc tttggaagaa ttgcctgaat acatcacgcg cggcgttttc gacttcatgt
    12781 tcggcatcga agccaaagtc cgccaaattc aagaagaagc caacattcaa gcggcggcga
    12841 ccatcggcag gctgcacgac gcgctcaaag aagaaggcat ttacgaagaa cacgagctgc
    12901 gcctcttcat cacgcgcctg cttttcctct tttttgccga cgacagcgcc gttttccggc
    12961 gcaactacct tttccaagac tttttagaaa actgcaaaga agccgacacg ctcggcgaca
    13021 agctcaatca actctttgaa tttctcaaca cacccgacca aaagcgcagc aagacccaaa
    13081 gcgaaaaatt taaaggtttc gaatacgtca acggcggtct tttcaaagaa cgcctgcgca
    13141 ctttcgactt cactgccaag cagcaccgcg ccttaatcga ctgcggcaat ttcgactggc
    13201 gcaacatcag tccagaaatc ttcggcacgc tcttccaatc cgtcatggac gcgcaagagc
    13261 ggcgcgaagc gggcgcgcac tacaccgaag ccgccaatat cgacaaagtc atcaacggcc
    13321 tttttttaga aaacctgcgt gccgaatttg aagccgtcaa agccctcaaa cgcgacaaag
    13381 ccaaaaaact cgccgccttc taccaaaaaa tccaaaacct gcaattcctc gaccctgcct
    13441 gcggctgcgg caacttcctt atcgtcgcct acgaccgcat ccgcgccctt gaagacgaca
    13501 tcatcgccga agccctcaaa gacaaagcag acggcctgtt cgacagcccg tccgtccaat
    13561 gccgtctgaa acagtttcac ggcatcgaaa tagacgaatt tgccgtcctc atcgcccgca
    13621 ccgccatgtg gctcaaaaac caccaatgca acatccgcac acaaatccgc ttcgacggcg
    13681 aagtcgcctg ccatacgctg ccgctcgaag acgccgccga aatcatccac gccaacagcc
    13741 tccgcacacc ttggcaggcg gcggactaca tcttcggcaa tccccccttt atcggctcga
    13801 cctaccaaac caaagagcag aaaaacgacc tcgaaagcat ctgcggccat atcaaaggct
    13861 acggcctgtt ggattacgtc tgcaactggt acgtcaaagc cgcaggcatc atggcgcagc
    13921 atccccaagt tcagacggca tttgtttcca ccaattccat ctgccaaggc cagcaggtcg
    13981 aaatcctctg gggcagcctt ttaaaccaag gcatcgaaat ccactttgcc caccgcacct
    14041 tccaatggac gagccaagcc gcaggcaaag ccgccgtcca ctgcatcatc gtcggcttcc
    14101 gccaaaagcc gccaatgccg tctgaaaaaa ccctctacga ctatcccgac atcaaaggcg
    14161 aacccgaaaa acacgccgta gccaacatca atccttatct gatcgatgcg cccgatttga
    14221 ttatcgccaa gcgcagccgt cccatacatt gcgaacctga tatggtcaac ggaagcaaac
    14281 cgaccgaagg cggcaacctt atcctttcaa ccgccgaaaa agatgccctg attgccgccg
    14341 aacccttggc ggagcaatac atccgcccct ttatcggcgc ggatgagttt ctcaacggca
    14401 aaacccgttg gtgcctgtgg tttcacggcg tatccgatgt caaacgcaac cacgacctga
    14461 aacaaatgcc ccaagttcaa gcccgtattc aggcggtcaa aaccatgcgc gaagccagca
    14521 gcgacaaaca aactcaaaaa gatgcagcaa ccccgtggct ttttcaaaaa atccgccagc
    14581 cttcagacgg caattatctg attattccga gcgtgtcgtc tgaaagccgc cgtttcatcc
    14641 ccatcggtta tctgtcgttt gaaacagttg tcagcaatct ggcatttatc cttccaaacg
    14701 ccaccctcta ccacttcggc atcctcagct ccaccatgca caacgccttt atgcgtaccg
    14761 ttgcaggtcg tctgaaaagc gattatcgct actctaatac cgtcgtgtac aacaacttcc
    14821 ccttccccga aagctgccgg ttgccgtctg aaaacgaccg ccccgacccg ctccgcgccg
    14881 ccgtcgaagc cgccgcccaa accgtcctcg acgcgcgcgg acaataccgc cgagaagcgc
    14941 aggaagccgg tttgcccgag ccgaccctcg ccgaactcta tgcgcccgac gcaggctata
    15001 ccgccctcga caaagcccac gccaccctcg acaaggcagt cgataaagcc tacggctaca
    15061 aaacaggcaa aaataccgac gacgaggcag aacgcgtcgc cttcctgttc gagctgtacc
    15121 gcaaggcggc ggcaattgcg tagtagttgc gtagtagttg cgtaggatag ttgcgtaggg
    15181 tgggcttcag cccaccaact cccaccaact cccaccatct tcaccctaaa accccgtcat
    15241 ctccgcaaaa gcaattgcgt agggtgggct tcagcccacc gtttttcttc attttcggtt
    15301 gttgattggc aggctgaagc ccaccctgcg gttacaaaaa tcccgtcatt cccgcgcagg
    15361 cgggaatcca gacctttcgg ggcggaaact tatcggataa aacgatttcc cctgcatcct
    15421 ggattcccac tttcatggga atgacgggat ataggtttcc ctacggacgt gttcagattc
    15481 ccgccttcgc gggaatgacg gcggagaagt ttctgtcttt ccgataactt accgtaactt
    15541 aaaatcccgt cattaccgca aaagcagttg ctacggttac aaaaatcccg tcatccccac
    15601 gaaagcgaaa atcctgcctg tcggatttcg gttttttcgg gcgtttcggg aacttataaa
    15661 tcgtcattcc cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat tcaaaggt
//
LOCUS       DAWFIT010000043        15654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFIT010000043 DAWFIT010000000
VERSION     DAWFIT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.043"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(16..771)
                     /locus_tag="WGQ85_001241"
     CDS             complement(16..771)
                     /locus_tag="WGQ85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0750664.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WGQ85_001242"
     CDS             complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WGQ85_001242"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0750665.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2481..4736
                     /locus_tag="WGQ85_001243"
     CDS             2481..4736
                     /locus_tag="WGQ85_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0750666.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5000..5704)
                     /locus_tag="WGQ85_001244"
     CDS             complement(5000..5704)
                     /locus_tag="WGQ85_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750667.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            5920..6183
                     /locus_tag="WGQ85_001245"
     CDS             5920..6183
                     /locus_tag="WGQ85_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0750668.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6261..8306
                     /locus_tag="WGQ85_001246"
     CDS             6261..8306
                     /locus_tag="WGQ85_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750669.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDVWVGDGHSFKAKIRHPMGYLFTPEVTMIVDGCSGAVVGW
                     SVALSETAVAVADALRHGMTHFPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFT
                     GIPGNPQGRGKIERLWQTITIPLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGK
                     ELSKAQEAALSAAPTWAQFIADLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYR
                     METDGMRVKTDVLSPLELDFMYRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVA
                     YDIHDADHVIVKDMQGKVICKAVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDL
                     IEAQRQSAAPIIEQQPDFGEFLKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8348..9262
                     /locus_tag="WGQ85_001247"
     CDS             8348..9262
                     /locus_tag="WGQ85_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0750670.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9338..9580
                     /locus_tag="WGQ85_001248"
     CDS             9338..9580
                     /locus_tag="WGQ85_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750671.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9570..9869
                     /locus_tag="WGQ85_001249"
     CDS             9570..9869
                     /locus_tag="WGQ85_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750672.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            9872..10024
                     /locus_tag="WGQ85_001250"
     CDS             9872..10024
                     /locus_tag="WGQ85_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750673.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10040..10204
                     /locus_tag="WGQ85_001251"
     CDS             10040..10204
                     /locus_tag="WGQ85_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750674.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10185..10400
                     /locus_tag="WGQ85_001252"
     CDS             10185..10400
                     /locus_tag="WGQ85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750675.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10397..10711
                     /locus_tag="WGQ85_001253"
     CDS             10397..10711
                     /locus_tag="WGQ85_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750676.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            10830..11207
                     /locus_tag="WGQ85_001254"
     CDS             10830..11207
                     /locus_tag="WGQ85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750677.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11191..11646
                     /locus_tag="WGQ85_001255"
     CDS             11191..11646
                     /locus_tag="WGQ85_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750678.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11618..11812
                     /locus_tag="WGQ85_001256"
     CDS             11618..11812
                     /locus_tag="WGQ85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750679.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            11805..12416
                     /locus_tag="WGQ85_001257"
     CDS             11805..12416
                     /locus_tag="WGQ85_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ0750680.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12422..13072
                     /locus_tag="WGQ85_001258"
     CDS             12422..13072
                     /locus_tag="WGQ85_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750681.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13073..13273
                     /locus_tag="WGQ85_001259"
     CDS             13073..13273
                     /locus_tag="WGQ85_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750682.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13289..13597
                     /locus_tag="WGQ85_001260"
     CDS             13289..13597
                     /locus_tag="WGQ85_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0750683.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNG
                     "
     gene            13673..14107
                     /locus_tag="WGQ85_001261"
     CDS             13673..14107
                     /locus_tag="WGQ85_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ0750684.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14104..14550
                     /locus_tag="WGQ85_001262"
     CDS             14104..14550
                     /locus_tag="WGQ85_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750685.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14598..15239
                     /locus_tag="WGQ85_001263"
     CDS             14598..15239
                     /locus_tag="WGQ85_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750686.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15371..>15654
                     /locus_tag="WGQ85_001264"
     CDS             15371..>15654
                     /locus_tag="WGQ85_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0750687.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTV"
BASE COUNT         4038 a         3457 c         4492 g         3667 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgcgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccaata gacgcgccgc tgcaaaacat ttgcgcattg gcatttccgg aggaaagcag
      181 gctgctgttg aaatcttccg tccattccca aataagtccg tgcatatcat aaacgcccca
      241 gtagttggga cggcccttgc cgacgtcatg caagcctttt cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gtaaacttca tatcatgcct
      781 cctgacaggc agttaaaatt gatttttcag acggcatttt aaacaaaaag ccgcgaatgc
      841 cgtctgaaga atcaatcttc cgaaaggaaa gattggttgt taaaaaacca ctgccgtgcg
      901 taattaagta cggcggcagt ggttcgtttg cctaaaaatg ggtgttcaat ttagtaatcg
      961 ctttttgcag gagcagaggc tgcaggagca gaagctgcgg gagcggaagc agcaggagct
     1021 gcaccgttac cggcgtaagc ggtatcactc aatttttgag tcatgatttc agggttttct
     1081 gcaccctcta ctttcaattg acccagtgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcaaccaaag tgtagctgcc cgggatgtcg actttgaatt cgacgatggc agagccgccg
     1201 gcaggaacga tggtgctttg tacgttttcg ttaatcagtt tgccgccttc aacataaact
     1261 ttgtcgaaga tttcaccgat tacgtggaag gaagacacca agttaggacc accgttacca
     1321 acgtacatac gtacagtttc gcctgctttg gctttcaggg cgttgtcgcc ggcgatagca
     1381 cctacgtgac cgttaaatac aacgtattca ggttgttcgg cgatggcttt gtccatatcg
     1441 aacggttgca ggccttgcgc gccttttttg cctttggtgt agaagtcgcc ttggacgatg
     1501 tagaactctt tatccacttt cggcagacct tctttaggct cgaccaaaat cagaccgtac
     1561 ataccgttgg cgatgtgcat accgaccggc gcgacggcgc agtggtagat gtacagaccc
     1621 ggttgcaggg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcggcc
     1681 gcgccgccac cttggccggt agctgcatgg aagtcaacgt tatggggaac ggtagaagaa
     1741 ggattgttag agaattctac ttctacggta tcaccttcgc gtacgcgaat catacggccc
     1801 ggaacgtcgc cgtcaaatgt ccagtagcgg tattccaccc cgtcgtccat tttcatggtt
     1861 ttttcgacgg tttccatttt tacgcgtact ttggcaggat agtcgcggtc gattgcggga
     1921 ggtacttcag gagcgtgggt ggtaactgca tcgatgacgg gcagttcgcc tgcaggcgtt
     1981 tcggcggcgg tttgtgcggc ggagcttgcg gcttcggcgg aagcggcagg ggtttgcgcg
     2041 gcaggttcgc caccgcaggc ggctaaagca aacatagaag cgataattgc ggctaaggct
     2101 tggcgtttca taatgttttc cttttgtaag aaaagtaggg agattggtta agaactggtg
     2161 tgattatgcg tttgccccat caccgacatt ttgatttaag tcaaataatt cataaaatat
     2221 gaacttaaaa tgatacttct aatactttat agctactaaa aattacaata aataggctga
     2281 tttgcttaag ggcttcggca aatcaggcaa aggcagggta gttgccgact aaagaataat
     2341 gatagttatt atcatatatg tatttgtttt atatgtgagt ttctttgaac aaatgtttat
     2401 ttgcgcggta aaccgtgcta caatctttaa cattcatatt ttgtgaattt taatccacta
     2461 tattaaaagg agctctcaaa atgggacagt acaagaagct gtggtacttg ctgtttgccg
     2521 ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat aagaaagccc
     2581 cgccttaccc cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata
     2641 ttttggcagg tcagtctgcg tggcagagta ccggcggtat ggaagtcggt tcgattctgg
     2701 gtcacggcgc atatcaggct ccggactgga cggcggactg gctgcatcgt gagttgtccg
     2761 cttggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa gtttcccctg
     2821 aagaacaagc cgttctgaaa acccgactgg ctgacgaata ccgcaaccaa agccgtatta
     2881 aagaagacgg cagcgtcgtc atcagcgata cgcgcgcgaa agccatcgaa agcatcctgc
     2941 cttattacca cggtgtttac ggcgacgatc ccgcgctgca aaccacccgc gaacactttg
     3001 caatgaaaaa caacacattg ccaagtcagg aagcgcgtga aaaactgttc gacttcttct
     3061 tctggacttc ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca
     3121 actggccgca cgagcctttg attaacaatg taccgactac tgaaaactac atgtggtcgt
     3181 ttaccagtgt tgtactgctc ttgatgggca tcggtttgtt gatgtggggt tattccttcc
     3241 tgaccaaaca cgaggaagtg gaagtgccgt ctgaagaccc gatttccaaa atccaactga
     3301 ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggttgccctg tttgtggtac
     3361 aagtattgct gggcggtctg actgcgcact acaccgttga aggtcagggt ttctacggca
     3421 ttgacgaggc gttgggcttt gaaatgtccg actggttccc ctacgccctg acccgtactt
     3481 ggcacatcca atccgccatc ttctggattg caaccggctt tttgacggcg ggtttgttcc
     3541 tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtaaacttcc
     3601 tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga
     3661 cgcacatcct tccgccggaa tttaacttct ggttcggaca ccaaggttac gaatacctcg
     3721 atttgggacg tttctggcaa ctcctgctga tggtcggcct gctgttgtgg ctgttcctga
     3781 tgttgcgctg cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa
     3841 tctttgtcgc ttctatggtc ggtgtgggcg tattctacgc accaggcctg ttctatggcg
     3901 aaaaatcccc gattgccgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg
     3961 aaggcttctt tgaagtattt gccactgccg cttttgcctt tgtcttctac aatatgggct
     4021 ttgtccgccg cagtaccgcc acggcctcta ctctggccgc tgccgccatc tttatgttgg
     4081 gcggcgttcc gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg
     4141 caatcggtgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg ggccgtgaag
     4201 catacgagca ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc
     4261 tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggtgta ttcggtttcc
     4321 tgattaaccc gccgatttcc ctgttctata tccaaggttt gaatacttct gctgttcatg
     4381 cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg
     4441 cccgttattt gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggc
     4501 tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gttgccggtt ggcgtgattc
     4561 aggcatacgc ctccatcacg cacggtctgt ggtatgcccg cagcgaagag ttcctgcaaa
     4621 tggaaatcct cgataccttg cgttgggtac gtacggcagc tgacttggtc tttatcggcg
     4681 gtgcaatctg cgttgccatc caagctacta aaatcgtatt cggccgcgac aaataagttt
     4741 tgcggtatga aatgagcaac ttatggggta ggtattattt tgacaccttt agaaagaaag
     4801 caaaagaagg cgaaaatttt aaaggaaaca aaaatttgaa cggaaattta ggaaaaagcg
     4861 gctttgacac ctttaggtgc aaagtaggta gaaagtgata taactttgat accttttttg
     4921 cacggttttg atgaggtttt gggttgattt tggttttatt tgaattgaac ggcgtttgga
     4981 atatccaaac gccgtttttt tagttgacgg tacggccata ccattctacg cgtccgatga
     5041 tggctacatt ttggttgtcg tcggttaaat ctatttcgaa aggctcataa accggattgg
     5101 ctgatttgac gagcagcttg ccgggtagtt tttggatttg tttgacaaac aggtgattat
     5161 cgatgcggat aacgtacagg ccgtcgcgcg gggtggtttc ggcgtgattc actaggatgt
     5221 tgtcgccgtg gttgagtacg ccctccatcg aatcgccctt tacggcgatg acggacagtt
     5281 tgtcaagctg gcgggtaacg tagttttcta tccaataacg cctgaatgcc atgcagaata
     5341 agggcttgtc gtcttctgcg ggatagccgt gccctgcggc ggcgtagacg tcgtaacgcg
     5401 ggataaagac gaactcgtct aagttgatgg ggttgccgag ggtatcggta gccgtaccgt
     5461 cggtatgggt acggacttcg accgcttggc ctttattcat gtatggcgag ccttggccgg
     5521 taagaagcca gtttaaatcg caaccggtta cttcttgaat tttaatcaga tagtctgctg
     5581 ttggaatggc accgtctttc cagacacggc taaaaccgga ggcagacata tcaatcttat
     5641 tgtagaagtc ggcgggtttg gcattgtccg gccacaaaga tttcaaacgc tctaaaaagt
     5701 ccatgattgt ccttttgctt agaatttcgc atttaagcaa aaatacactt cttttgctta
     5761 aaattaattc gtcaaataat caaatagatg gctaatattt tgcttttttg cgttatattt
     5821 ctaagcaaaa gagttgcaat aacctttttt gcttagtatt attcactcac ttaatcaatt
     5881 aacgaagtaa atcacattac tgaatggaat aagtcaatta tgcaaaaaaa cgcaacgccg
     5941 aaaaactggc accgcgcgga tattgttgct gctttgaaaa agaaaggctg gtcgcttcga
     6001 gcactttcaa tagaagcggg gttgtcgccg aatacgctta gaagcgcact ggccgcccct
     6061 tatcttaagg gagaaaggat tattgccgct gcaatcggag tggaaccgga agagatttgg
     6121 cccgaacggt atgcagatcg gaatttaaaa cctgttttcc ccaaaagagt agttaatgga
     6181 taagtggttt tcgatttagt tccttaaatg ctaaaggtta tacgcattta agtaaaacac
     6241 aaaaagaaag aaatagcaaa atgttgattt cagcggttga gttggcgggc ttgcaacttc
     6301 caaagctgcc gaacagtaga caaggcatcg agtaccacgc caaaaagaat aattggccgt
     6361 ttgaagaggt tgtaggacag gctcgaggcg gcaagcttaa gaaatatttg gtttccgccc
     6421 tcccttcgga gattcaggcg gccatacagg aaaaacaggc ggcaacattg ttggccaagg
     6481 tgccgatgtt gccggcagaa gtaaaaaaac cgcttcggca aaataaaaag atgcggcagt
     6541 tgggtttgat accgtgcgag gaaggtttgg cgcggttgga cgacaggcag acggaaacgg
     6601 cccacgcgcg ttgtgcgatt gtcgcctatg tattgccgct gcatgaattg gcgggtatgc
     6661 cgattaagaa ggcggtggcg tttgtggcag ctgaagcggc agcaggcagg ttgccggagg
     6721 atgtggccaa gctgattccg ttggcgaatg cgcggaacaa cggcgaacgc agtttaagcg
     6781 aaccgacttt ataccgatgg gtgcgggctt accgtgcggc atcggacagt atgagccgcc
     6841 tcttggcact tgcgccggtg aaaacacggg aaaagacgcc gctgctggcg attgactggc
     6901 tgccttactt cttgatgttt tatcagcggc cgaacaagcc gacgatgatg gcggcggcga
     6961 agaaactggc gcaatggtat ctggaacagg gaaaaatcga gcagatgccg agctatgacc
     7021 agattcaaac agtgatgaaa cggttgccgg aacatatgaa ggagcgcggc aggcgcacgg
     7081 ggtcggctta taaggctttg ctgccttaca ttgatagaga ttggacggcg ttgaaaccga
     7141 acgatgtctg ggtgggcgac ggccacagct ttaaagcaaa aatccggcat ccgatgggct
     7201 atctgtttac gccggaagtg acaatgattg tggacggttg cagcggtgcg gtcgtgggtt
     7261 ggagtgtggc gctgtcggaa acggcggtag cggtggcgga tgctttgcgc cacgggatga
     7321 cgcatttccc gccacctttg ggttattact cggataacgg ctcgggtgaa acgggcgata
     7381 tgctggataa ggaaacgacg ggtattttgc cgcgtatcgg tatcgagcat tttacgggca
     7441 ttccgggcaa tccgcagggg cgcggcaaga ttgaacggct gtggcagacc attacgattc
     7501 ctttggccaa gcagtatgcg acttatcagg gcaaggatgc ggatgccgaa acattgagaa
     7561 aggtatcgaa tgcgctggcg agtgcgcaga aggtgcagaa aaagggcaag gaactgagca
     7621 aggcgcagga agcggcgctg tcggcggcac cgacttgggc gcagtttata gcggatttgg
     7681 aagaagcggt acgccgatac aactttgaac acgagcaccg cagcctgccg aaaaatccgg
     7741 aagcgggacg gcattttacg ccgatgaaat attacgaata ccggatggaa acggacggca
     7801 tgagggtgaa aacggatgta ttaagcccgc tggagttgga ttttatgtac cgtccggagg
     7861 aagaacgcat ccccgaccgc ggagcggtgt ctctgcacaa taatacttac ttccaccaag
     7921 aattgctgga ttacagcggt cagaaggtac gggtggctta cgacatccac gatgccgacc
     7981 atgtgattgt gaaggatatg cagggcaagg tgatttgcaa agcggtattc aacggcaaca
     8041 aacgggcggc atttgcggaa acgcggatgg aacaactggc cgaacgccgc cgcaaaggcc
     8101 aggccaaacg tctgcaagac aagatggatt taatcgaggc acaacgccaa tcggccgcgc
     8161 cgattatcga gcagcagcct gatttcggcg aatttttgaa gctggaaacg gaaagcggcg
     8221 ggttggtaga ggtggaaaac aggccgtctg aaagcggcag gggcaagaag aagttacgcg
     8281 attttttatg ggaagacgcc ggttagtaaa acggcacatc tttgacactt tttaaggaaa
     8341 acgaaaaatg attgagaaat taagacaatt tctggaaacc agcggcatga gccaaaacaa
     8401 ggcggccgac cgcatgggcg tatcgagaag tgcattgtcg ggctatttga acggcaaata
     8461 cgacggcgat attgcgggga tggataagaa agccgcgctg tttcttgaac aggaaggcga
     8521 ccgggcggaa ttgaaaaagc tggatattcc ctatgttgaa acgggcacgg ccaagaagat
     8581 gaaaggttgg ctgggtttgg cggcatggct ggggcagctc ggtattgtgt atggcggtgc
     8641 gggcttgggt aaaaccacgg tattgaaaca atatgcggcc actaacccgc tggctttgtt
     8701 gattgagccg gatacaggct atacggcaaa ggtgttgttg caggaaatct gccatgcact
     8761 ggatttaagc gagagaggca atatccatga gctgacggag cggattatca actgcctgaa
     8821 gcgtgataag aagagccgca gcccgcgtga tgcacaccgt attttgctga ttgacgaggc
     8881 ggaacagtta ccgacacgcg cactggagag cctgcggcgg atacatgata aatcgggcgt
     8941 ggcggtggcg ttggtgggta tgccaaagct gctgctgaat ctgaaggggc cgaacagtga
     9001 attcaagcag ttgttttcaa gggtatcggt caagatggaa ttgggcgaga tgctgccgga
     9061 aacggattta aaacagattg cggcggcagt gttgaagacg aacgatgagg cggtattgca
     9121 gaaggtggtg aaaacggcca aaggcaatgc gcggaaactg tcgaagctgc tgctgattgt
     9181 ggactatttg ttgcaggtta accctgatgt tgatttggat gatgatgtaa tcgaacacgc
     9241 ggaaacctat ttaatccact aaacctttga cagataaggc aataattttt ttaccttgtt
     9301 attttgttaa gttattgaaa ttaaaggaaa actaaaaatg gaaaatttga aaatgcggca
     9361 tctgctcgcc ataccggcga ttgtgattgc ggtaacggcg tgtgcgaacc gctgcaccga
     9421 gccggtgccg gcgtggagcc tgtatcagaa cgagaccagt cagcagcggc tgatgcggga
     9481 aaaacagaat gcggctgact ttgcggtatt gcaggcagaa aaagcctatg aacggatgag
     9541 cgataaagag cggatgaaag gggtggttta tgaaaactga gacgaaaatc tgcaaaaaat
     9601 gcggagaaga gaggccgttg ttccgtttca aaaaagacga tgcgtatgcg ggcgggtata
     9661 ggcctgtatg ttacgagtgt ttaaacgcca gagcaaagga actgaaggca gtccgagaca
     9721 gtatggcgga aagaaagatt atctctaaag gcatcagcat aaatggaggg aaaacgcata
     9781 tggaatttcc tgaagaacct gaatttatga aaaacaaata ctggaccgtt acaaaaacgg
     9841 attatagaag accattaaaa ggactttaac catgagtaaa cggttggcac gacggaagat
     9901 gccgaaacac gaattgaaac gggttaaagc gttcgccctg aaacaggcgg tcgagaagat
     9961 tcgggcacgg tggggcgaga aagcgatacg gcttgcctca acagacaaca aacagactga
    10021 ttaaacaagg aataagaaaa tgatgaatga aatgaatgcg gtagcggctt tgttggttat
    10081 tgccatattg ctggcggcgg taatgactgt gttggtcgag atattggtcg agcaacgggt
    10141 taaggaacgg ctggggaaac tgctggataa ggcgtgccga ccggatgacg ggtggcagga
    10201 atgataagga aggaaatgtg atgaatatgg tactgacaat ttatttttgg ttggtggggc
    10261 tggtctgttt tgcccgttcc gccaaagaga cttattacgc ggaatcttta tacggtctgc
    10321 tgactgggct gttgggggtg atgtgttcgg cttttgtggt gtcagtgtcg gtggcaaggg
    10381 tgatggggtg gttgaaatga atcaattgaa aatgacgatc gccaaacctg agacggaaga
    10441 ttttgaagat gcctgggcat ttatcagaat gcttaatttg gtgacttatg atttgaaccc
    10501 tctgaaaact gataccgacg gcgaatacga atatttggcg gatgaagaca aatctgatgt
    10561 attagatgcc gttgtcgaaa aatttaacga atgtagtctt gagtggatgt tgtcggcgct
    10621 gcaagcactg atgtcgccgg agatggggat tatcaaccaa gattcggata cgttggagtt
    10681 gcatcccaaa ctgaaaggcg gtacggaatg aaagtgcgct gccctacctg cggtgcggtg
    10741 atgagcttag atgtattaat cgcccatgat gatgcagcga agctctgatt gccctgacag
    10801 gcatttcaga cgaccttttt aaggcggtat tgcggtatct gacgctgttt cgccccgccg
    10861 aaaaggattt aagttttaac cgagtttcaa agctcgtcgg cgagattgcg ccgatgatac
    10921 gggacggaaa gattgtccgc aaccgcaaaa cttatccggc cccgcgcgag gcttggattt
    10981 gggcggcaac gcgatgcctt gaggcacggg acgcgggaaa actgacaccg ccactgacca
    11041 gccacggtta tttgttggaa aacattacgt tttggtcgcc tgaaaagacg gcgggaacgg
    11101 cggttttgcc ctctccccaa ccatctcaca cgagagaggt ggcaagcacc aaattgagga
    11161 gcgggttggg cggcttgatg gagtggtcaa atggaggaaa acaatagctg gctgaaaaaa
    11221 gcaatcgcgc agggtttcat gatgctcgcc gccctaaacc tcaaaggccg ccctgcctcg
    11281 gcggatttga cggcagtcgc cgaactttgg ttggtcatac taagcggccg gtcgtggcag
    11341 ccggagcatg acgggatcag gatacaggca gcctttaggg ctatcgcggc gtcctcgtca
    11401 gagtggccaa accctgccga ccttatcaag cacctgccac cgggcgaagt caggatggtg
    11461 ccgaggctgg aaaagaagca ctgcccgacg gaatacggca aagagcaggc cgccgaactg
    11521 aaaaagattg tcggcagatt gaaaaacgca ccctgcatga atagagattg gatacacggg
    11581 caacgccacc ggtcggtgga cgaatgtaaa aggatttatg ccgaaaggca gaagggaaat
    11641 aaatagccaa atgagtacgt tttttgacaa tcaggaatcc gacttaataa acgaatttct
    11701 gacggaatac tgggaagagt ttgaaaaatt ctccgccaac aacggatttg atgacgattt
    11761 attgaagcaa atatcaatgg aattggaagg acataggaac cattatgagt aacttagaca
    11821 tgaaacaata caaacaggat gccaaaggca atctcgtgcc gctggccaat attaaagaaa
    11881 tcgacctgct gcgcgacgag ctggtgcagg aaattgccgc caaagcccgc gcggtacagg
    11941 ataacttgat ggcgttcaaa cgcgaggcga tggacgatat cgcagcgttt gtacagttga
    12001 gtgccgaccg ctatgacgta tctgtcggcg gtaagaaagg caatatcagc ctgcacagct
    12061 ttgatggagc gtaccgcgtc aaccttgcca tacaggacac gttggtattt gacgaaggtt
    12121 tgattgccgc caaagccctg attgacgagt gtatcaacga atggacggaa ggcagtcgca
    12181 cggaattgaa aacactgatt aacgcggcct tccaagtgga caaagaaggc aatatcagca
    12241 ctgcccgcgt cctcggtctg cgccgcctgc aaatcacgga tgaaaaatgg caacgggcga
    12301 tggatgcgct ctccgacagt ttgcaggtgc atatcagcaa accgtttgtg cgggtatatc
    12361 agcgcggcga ggatggggag tatcagttaa tgaatttgga tgtggcgaag gtgtaggaaa
    12421 aatgaacgca aatataatga tttatttgat tgaagttaat gatgggccgg ttgcaaaagc
    12481 actgcgaagt tttcagcagg acaaggccgc aatgcggcag gcgtggcttg attgggcgcg
    12541 agaacacttg ccgtcagacg cgctaatgcg ggaatggagc gatgggcaag tggcagggtt
    12601 tgcgttccct tcaggcatac ccgatggatg gaaaaagccc aataagaacg gaatttgttg
    12661 gccacggcaa aacaacccaa tcctcaaaac catgccactt aacaaaagat ttaaacgccc
    12721 cgaagagtat ttggaagaag ttggcatcac cgcaccaaca atgatttttg aaaagaatag
    12781 cgacggagag acttgggcat cttggggtat cggtaatttc tttaatcctg ttcagttcgt
    12841 ttgggcggga ttggaggagg acgcgccaaa gggcgtggta actcctgatt atgcctacga
    12901 gcttagagaa ggggctaaga gaatcagaaa cggctgtacg atgcagccgc ccgaagattt
    12961 tgattggcag aatctgttac cgggctgccg tgtcattcct cggtatgagt gggattactt
    13021 ggttgggaaa tggcaggaaa cacggaatga tgccgaagaa caggaggctt aaatggcaaa
    13081 aatcattatt gaaatcgaag acctgcccga gggcaccgac attaatttta agggcgacct
    13141 tccggcggcg gatgcaaaag acaaaacagg tgcgcagcaa acggctgtgt taatcagcaa
    13201 gatgattcag gcggcgcaga tgatggcatc cccatccgta aacattaggc cgtctgagac
    13261 tgcaaatcca taacccgcgc ggcacggtct gccgcattta aatctaaata ggagtcaaaa
    13321 agtgaataaa tccgaattaa tccaagccat cgccgacgag gcggaattga gcaaacgcgc
    13381 tgcggcggag tttgtcaatg cgtttgtcag cgtggtaacg caagaactga aagacggaaa
    13441 cgacgttacg ctggtcggct tcggcacgtt ccacgccgcc caatccgccg agcgtcaggg
    13501 acgcaatccg aaaacgggcg aaccgctgac catcgcggcg cgcaaaacac ccaagttccg
    13561 ctcgggcaag gcgttaaaag atgcggtaaa cggttaaacg tctgaaaccc caaatccaag
    13621 cggatttttg aaaagtccgc ttcagtttga agttttagcg acgaggaaaa ccatgatgaa
    13681 cacaaacgaa cagtacaaac gcaaggggct gattgcgaaa atcaaaatcg cgcaaagcca
    13741 gcttgggatg gaagacgatg tttaccgtgc gatgctggcg cgtgtgtgcg gcaaaacatc
    13801 ctgcacgcag atgaatctag ccgaattgca ggcggtggcg gcggaaatga agcggatggg
    13861 ctttaagcag accgcaccga agggcaaagg tatccgcccg catttgacac aggaccgtgc
    13921 cgcgctgttg aacaagctgg aagcgctgtt gacggtgggc gataaaagct ggcagtacgc
    13981 cgacgggatg gccaaaagga tgttcggcaa ggatttggta cgatttttga cacccgagca
    14041 gctatacaaa ctggtgcagg cgctgcaaat ccatatcaac aaagcgaaaa aggcggcaga
    14101 gcaatgatca gggtggatga agaagatttt gaggcggtgc gccacctgct gcccgaaagt
    14161 gtgctggcac tgattacgat catcgggctg acagaaacgg tggagctggt gaaaaactta
    14221 ggcggcacga cctacccgtt gcggcaggga cgcaccaaag gcagcgaatc gcggctggct
    14281 tatttggagg aaatcgtcgg cggtgcggcg atggaaaaaa tggtggaagc actggccccc
    14341 tgcgatttgt ttattccgaa atgcgagcag gctttgctgg aattgcgtga ccgccatatc
    14401 cgccgccgct tcgatgcgca gacgggtaaa ggcgtaccgg cttatgaggc ggtgaacgat
    14461 ttggctttgg cgcacgcgct gaccgaccgc catatctggc ggattctgaa gaagccggat
    14521 aatgagtgtg agcaaggcgg gttgttttaa attataatgc cgtggggata acacggcatt
    14581 ttttatggga aatcaagatg aagacgtttt atgttctgat gttggccgca atgcttgcgg
    14641 cttgcggcgg acaagaaaac aagcctgctg ccggcgaaag tcaaaccgat aatcgggcgc
    14701 agcaatatca gaatcagcaa aaacagattg ccgcacgttt ggctgatgaa gaatatttca
    14761 tcggtgaaaa caatttggaa caaatccgtc tgcacgccaa tttgaaagag cgcgctcaaa
    14821 agctgttgtc tttattggcg caggccgaca aggaaagccg tgtgtgggtt ttgcccggcg
    14881 atgcagccaa aatcaaagaa ttcaatgcgg ctttcgcggc ggtggcaaaa tctgccgagg
    14941 aatctttcgg cggccctttt cttgcggata aggcggggct ttatcaatgt accgctgcgg
    15001 ccaatgcggc ttacgattat tttttcgccc ggcagagaaa ggatgcgctg accgaaaatt
    15061 accgtcaaca atacacggac aacatagctg cctgccaagg gcaaatccgc accccgccag
    15121 ccgcccaagc gacagtgtat gcgcgtaaag gtatcaggct gccgttgaat aattgtttgc
    15181 cggtattggc cggagatgaa gaatttgata cctacacctg cccgatggaa gtaaaatagg
    15241 ccgtctgaaa cccagcccga gagcaaaagc cctcgggctt tttgctgcct gtctgacatg
    15301 gggcaggcgc ggtgccaaat aagcggacag gataatcaag gctcgataac agactaagca
    15361 ggacgaatct atgagcaaga ttattgtgct gaccgcaggc cacagcaaca ccgacccggg
    15421 tgcggtcaac ggaagcgacc gtgaggcgga cttggcgcag gatatgcgca atatcgtggc
    15481 tgctattttg cgcgatgact acggtttgac tgttaaaacc gacggcacgg gcaaaggcaa
    15541 tatgccgctg cgcgaagcgg tcaagctgat tcgcggctcg gatgtggcga ttgagtttca
    15601 caccaacgct gccgtcagca aagcggcgac aggcatcgaa gccttgagta ccgt
//
LOCUS       DAWFIT010000044        15325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFIT010000044 DAWFIT010000000
VERSION     DAWFIT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.044"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..149
                     /locus_tag="WGQ85_001265"
                     /pseudo
     CDS             1..149
                     /locus_tag="WGQ85_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            441..1595
                     /gene="obgE"
                     /locus_tag="WGQ85_001266"
     CDS             441..1595
                     /gene="obgE"
                     /locus_tag="WGQ85_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0750688.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1678..2727
                     /locus_tag="WGQ85_001267"
     CDS             1678..2727
                     /locus_tag="WGQ85_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0750689.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3415..4836
                     /locus_tag="WGQ85_001268"
     CDS             3415..4836
                     /locus_tag="WGQ85_001268"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0750690.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGVASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4919..5698
                     /gene="xth"
                     /locus_tag="WGQ85_001269"
     CDS             4919..5698
                     /gene="xth"
                     /locus_tag="WGQ85_001269"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0750691.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIATSGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5685..6029
                     /locus_tag="WGQ85_001270"
     CDS             5685..6029
                     /locus_tag="WGQ85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750692.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            6039..6569
                     /locus_tag="WGQ85_001271"
     CDS             6039..6569
                     /locus_tag="WGQ85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0750693.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWRAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6662..7777)
                     /gene="asd"
                     /locus_tag="WGQ85_001272"
     CDS             complement(6662..7777)
                     /gene="asd"
                     /locus_tag="WGQ85_001272"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0750694.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAKVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7953..8534
                     /locus_tag="WGQ85_001273"
     CDS             7953..8534
                     /locus_tag="WGQ85_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0750695.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8596..9450)
                     /gene="folD"
                     /locus_tag="WGQ85_001274"
     CDS             complement(8596..9450)
                     /gene="folD"
                     /locus_tag="WGQ85_001274"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0750696.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9673..9750
                     /locus_tag="WGQ85_001275"
     tRNA            9673..9750
                     /locus_tag="WGQ85_001275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9708..9710,aa:Pro,seq:tgg)
     gene            9775..9851
                     /locus_tag="WGQ85_001276"
     tRNA            9775..9851
                     /locus_tag="WGQ85_001276"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9809..9811,aa:Arg,seq:tct)
     gene            9879..9954
                     /locus_tag="WGQ85_001277"
     tRNA            9879..9954
                     /locus_tag="WGQ85_001277"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9912..9914,aa:His,seq:gtg)
     gene            10089..10595
                     /locus_tag="WGQ85_001278"
     CDS             10089..10595
                     /locus_tag="WGQ85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0750697.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10592..12370
                     /locus_tag="WGQ85_001279"
     CDS             10592..12370
                     /locus_tag="WGQ85_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0750698.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDEQGVLHLETAEGKQTVVSGEISLRPDNRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12483..13307
                     /locus_tag="WGQ85_001280"
     CDS             12483..13307
                     /locus_tag="WGQ85_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0750699.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14070..14858)
                     /locus_tag="WGQ85_001281"
     CDS             complement(14070..14858)
                     /locus_tag="WGQ85_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0750700.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15072..15266)
                     /gene="thiS"
                     /locus_tag="WGQ85_001282"
     CDS             complement(15072..15266)
                     /gene="thiS"
                     /locus_tag="WGQ85_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0750701.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3694 a         3868 c         4219 g         3544 t
ORIGIN      
        1 caagcaaaac gtcatttacg caagtttaac ggcattccca aagcgcattt tgagctgtat
       61 ttaaaggagt gcgaatggcg ttttaacaac agtgagataa aagttcttgt tccattttaa
      121 aacaattagt aaaaccgagt ttatcctagt tatctaggac agccccaaaa cttattatat
      181 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      241 agaaggtgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      301 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      361 cgaaattctg ttcggacggc atcaaaacac acgccatgcc gtctgaaccc ccatttgaaa
      421 ccaacaaaca agagcattga atgaaattca tcgacgaagc aaaaatcgaa gtcgccgcag
      481 gcaaaggcgg taatggcgca accagtttcc gccgcgaaaa attcgtaccg cgcggcggcc
      541 cggacggcgg cgacggcggc aaaggcggca gcgtttgggc agaagccgac gaaaacacca
      601 acaccctcgt cgaataccgc ttcgtcaaac gctaccaagc caaaaacggc gaaaaaggcc
      661 acggttccga ccgttacggc gcaggtgcgg acgacatcgt cctcaaaatg cccgtcggca
      721 cccttatccg cgacctcgac accggcgaaa acgttgccga cctcacttat cacggacagc
      781 gcgtctgcct tgccaaaggc ggcaaaggcg gcttgggcaa catccacttc aaatcgtccg
      841 tcaaccgcgc cccgaaacaa tccacccccg gcgaagaagg cgaagcccgt tccctgcaac
      901 tcgaactcaa agtcctcgcc gatgtcggct tattgggtat gcccaacgcc ggcaaatcca
      961 ccctgattac cgccgtatcc gccgcacgcc ccaaaatcgc caactacccc ttcaccaccc
     1021 tgcatccaaa cttaggcgtg gtacgcatcg acgaaaacca cagcttcgtg atggccgaca
     1081 tccccggcct gattgaaggc gcggcagaag gcgcaggcct cggccatcgt ttcctcaaac
     1141 acttatcacg taccggcctg ctgctgcacg tcgtcgattt ggcgcccttc gacgaaaccg
     1201 tcaaccctgc cgaagaagca cttgctatca tcaacgaatt gcgcaaatac gatgaagaac
     1261 tttacggcaa accgcgctgg ctggtgctga ataaactcga catgcttgac gaagaagaag
     1321 cccaaacgcg cactgccgcc ttcctcgaag ccgtcggctg ggactacccc aaacccgacg
     1381 accgtttcca atttgacatg gaaaccccgc gcctcttcca aatcagcgcg ctgacccacc
     1441 aaggcacgca ggaattggta caccaaatca accaatacct gaccgagaaa aaacgtatcg
     1501 aagctgaaaa agccgaagcg gagaaagcag cggcaaatgt tgaaattatc gaacagcagc
     1561 ctaaaacgga tacgggcgtg tttaagccgg agtgaaaaaa gaaagggttt cagacgatat
     1621 ttgttctaca aaaggttgtt tgaaacaatt ttaaagcaag attgaaatgg attaaaaatg
     1681 tcatctaacc aatatatcca gtcgcttgag ttgacaaact ttaccgttct tccaaacgat
     1741 acttttgaat tttctgaaaa tctcaatgtt attgttgccg aaaacggttg tggcaaaaca
     1801 catttgttaa aaattttata cagcctgcta gaagtaacat ctaacaccaa aaacagatta
     1861 ctgaaaacag aattgcaaaa aagttttgcc gataaattac tgaatgtttt ccgtcccgat
     1921 tccttgggca ggttgagcaa acgtttgcag gggcgcgggc gtacggaaat cgttttaaaa
     1981 ctgcaaaacg gcactacgca tagtagtctg aatttttcca gtaactcatc atcgcaagtc
     2041 aatgttcaaa gcatcggatt gaaagaaaat gaatatactc cgacacctat cttcttaccc
     2101 agccgcgaat taattacact ttgcccttgg ttcacttctt tgtatcaaaa ccaaagcatc
     2161 cctttcgaag aaacttggtt tgatacctgt atgcaattaa atcacccgct tgccaaaggc
     2221 cccagagaaa caaaaattcg agaattgctc gaacctattg agaacgcgat gggaggaaaa
     2281 gtatctgaag aacaaggaag gttttatcta tcgctgacaa atacgggcgg taagattgaa
     2341 gcacctttgg tagcagaggg tttgcggaaa tttgtcatga ttgcccggtt aatcgcaaca
     2401 ggggcattgc tggataaagg ctatctattt tgggatgaac cggaagctaa tctgaatcct
     2461 aaattgatca aagtggctgc ccgtattatt tggtctttat ctcaacaggg tattcaggtt
     2521 tttattgcaa cacactctct attcttattg agagaattgg aacttttgaa gcttgaaaat
     2581 aacacggatt tacctgtacg ttttttctcg ttgattgctt ccgatgatgg aacaaaagtg
     2641 gagcaaggga acagtattaa tgatttgaac acattgatat tgttagatga aaacattatg
     2701 caggcagaca ggtatctgaa tatttgaggt taagctatga ctacaatcac agaaaagaga
     2761 ttaacctttg cttttcctga agattattac gttaccaagt atgacgaatg ggaacattat
     2821 aaaatttttc agaacagttg taatttacgt aataaaattg atacaaatga aaagggaaag
     2881 aacgggataa atcaaagtgt agatgacgat aatggcagct caggtgtaga tataatagcc
     2941 cttcatgagt cgacactttg gctaatcgaa attaaagatt attaccgact tgggcttgag
     3001 ccaaatgccc aatcaataga tgaaaaactt tctgacctac catatttaat cgcaagaaaa
     3061 attcgagatt cattggcagg attggtttct gctaaattta aagcagaaaa acaggaagaa
     3121 aaagattttt ctcgcttggc tttagattgt aacgaaataa aaatagtcct tcatattgaa
     3181 atgcctagta taagaagtaa actttatccc tcgtcatcag atttagcaaa cttattaaaa
     3241 gataagttta aattatcaga atttacaaaa aattttgcaa actgttatgc agaaccaatt
     3301 ttcacaaata tcagccatat taataatccg caactacgta atgttccttg gtccgtatct
     3361 acaggaacag aacagaaatt atcatccgag caacaaagat taatccataa tcccatgacc
     3421 accatctaca acacccttac ccgccaaaaa gaaccctttg cccccatcga ccctgaaaac
     3481 gtgcgtatgt acgtttgcgg catgactgtt tacgattatt gccacttagg ccatgcccgt
     3541 gtgatggttg tgttcgacat gattgcccgt tggttgcgtg agtgcggcta tccgctcact
     3601 tatgtacgca acatcaccga catcgacgac aaaatcattg cccgtgcggc tgaaaacggc
     3661 gagaccatcg gcgagctgac tgcgcgtttc attcaggcta tgcacgaaga tgccgatgct
     3721 ttgggtgtgt tgcgtcccga tatcgagccg aaggcgacgg aaaacattcc gcaaatgatt
     3781 gccatgattg aaaccctgat tcaaaacggc aaggcatatc ctgccgcaaa tggcgacgtt
     3841 tactacgccg tgcgtgagtt tgccgcttac ggacaactgt cgggcaaatc gttggacgac
     3901 ctgcgcgcgg gcgaacgcgt ggaagtggac ggtttcaaac gcgatccgct tgattttgta
     3961 ttgtggaaag cggcgaaagc aggcgaaccg gcgtgggaaa gcccgtgggg caacggccgt
     4021 ccgggttggc acattgaatg ctctgccatg agtgaaaacc tgttcggcga taccttcgac
     4081 atccacggcg gcggcgcgga tttgcaattc ccgcatcatg aaaacgaaat cgcccaaagc
     4141 gtcggtgcca gcggtcatac ctgcggccac gaccacgcac aaacccacca cggtcaaagc
     4201 attgccagcc acgtcaaata ttggctgcac aacggcttta tccgcgtgga cggcgaaaaa
     4261 atgtccaaat cactgggcaa cttcttcacc atccgcgaag tgttgaaaca atacgacccc
     4321 gaagtcgtgc gcttcttcat cctgcgcgcc cactaccgca gcccgttgaa ctattccgac
     4381 gcgcatttgg acgacgcaaa aggcgcgttg acgcgtttgt atacgacttt gaaaaatacg
     4441 ccgcccgccg atccgatgcc gtctgaagcc ggcgatgact acacgcgccg cttttatgtt
     4501 gcgatgaatg atgattttga tacggtgaaa gcggtggcgg tgttgttcga acttgccggc
     4561 gaagtgaaca aaaccaataa cgcgcaactg gcaggccgtc tgaaggcttt gggcggcatc
     4621 atcggactgc tgcaacgcga tccgaccgag ttcctgcaag gcggcgtggc ttcagacggt
     4681 ctctccaacg aagaaatcga agacttgatc gcccgccgca aacaggcgcg ctccgataaa
     4741 aactgggcgg aatccgaccg catccgcgac cttctgaacg aacacaaaat cattttggaa
     4801 gacaacgccg gcggcacgac ttggcggcgc ggttaaaatc catcgaaaag ggtaaatgtg
     4861 ccgcattttg cccttttttg ttcagacggc atttttattg tgagaactta tttttattat
     4921 gcttaaaatc atttccgcca acgtcaacgg catccgctcc gcctacaaaa aaggttttta
     4981 cgaatacatc gccacatcgg gcgcggacat tgtctgcgtg caggaactca aagcgcagga
     5041 ggctgatttg tctgccgata tgaaaaatcc gcacgggatg cacggccatt ggcattgcgc
     5101 cgagaagcgc ggttacagcg gcgtggcggt gtacagcaaa cgcaaacccg acaatgtgca
     5161 aatcggtatg ggcattgaag aattcgacag ggaagggcgg tttgtgcgtt gcgatttcga
     5221 caggttgagc gttatttcgc tttacttgcc cagcggcagc agcgcggaag aacgccagca
     5281 ggtgaaatac cgtttccttg atgcgtttta ccctatgctc gaagcgatga aaaacgaagg
     5341 acgcgacatt gtcgtttgcg gcgactggaa catcgcccac caaaacatcg acctgaaaaa
     5401 ctggaaaggc aatcagaaaa attcgggctt cctaccggaa gaacgcgagt ggataggcaa
     5461 ggttatccac aagctcggtt ggacggatat gtggcgcacg ctttatcccg atgtgccggg
     5521 ctacacgtgg tggagcaacc gcgggcaggc gtatgcgaaa gatgtcgggt ggcgcatcga
     5581 ttatcagatg gttacgcccg aacttgccgc caaagccgtg tccgcacacg tttataaaga
     5641 tgaaaaattt tccgaccatg cgccgctggt catggagtat gactatgctg ccgaataaag
     5701 ttttgggtaa atatgattgg aatgtggacg gaaaaacagg aataggcgca gcatgggttg
     5761 tcgctgcgtt tatcctgccc atattggtgt gggcgatatt tatgttgtcg cggatgcaag
     5821 gctggcttgc gccgaccaag gcaaacccga catgggcgtt ggtgtggctg ctgatctgcc
     5881 tgccctgcct gctgattgcg tccaaatgtt tgggttggaa aggctggcgg ctgttggcaa
     5941 acgttcttgc ctgcctcgcc gtgtgcgctc tgttgagtgt gcctgccgcg ctgctgattg
     6001 cgttcacgct gcaagacctg ctcaaatagg cgcggacaat ggaaagtttc gagcttgagg
     6061 atttgacgct gtggctggta cgcgatgcgg atgaagcgga aatgtggata gaccgctggg
     6121 cgatcagtta tcccgtcgtg cagatgtccg aagcgtcggc cggtcaaagc ataggggaat
     6181 ggcgggcagg gcttcagacg gcatttgaac gcatacgcgg caaatacgtc gccgttgtcg
     6241 cacacggtgc gggcgcggcc gcatttttgg cgtggctgta tcaggcagac atcctgacac
     6301 ggaagaaaat tgccaacatc atccttgtac cgcagcgtcc cgatattttt cccgacgatg
     6361 cggaacacac tttccaacgc gtccgctgtc cctgccgtgc cgcattggtt gtacccgaac
     6421 acggcggcgt gccgcacggt tgggcgcaaa aacaggcgga tttgtggaac gcccgcctgt
     6481 tggtttcccc gcattcgggc agtttgaacg gcatgctcgg cggctggcag tggggtatga
     6541 agctgatgca ggaaatgttg ctggcgtgaa cgcttgcctt atcggaattt cggttaactg
     6601 gggttgcaag gaaaatgccg tctgaaacag gcttcagacg gcatttccat tcaaacaggg
     6661 cttacaggct gcccaacacg atacgcagca cgcggcgcaa cggctcggca gccccccaca
     6721 agagttggtc gccgacagtg aacgcgctga tgtattcgcc gcccatctcc agtttgcgga
     6781 tacggccgac aggaacagac agtgtgccgg taactttggc aggagtcagc tcgtgtatgc
     6841 tggcttcttt ttcgttaggg atgactttca cccagtcgtt tgcacctgcc aaaatcgctt
     6901 cgatttcgga aacaggcagg tcttttttca gcttcagggt gagggcttgg ctgtggcagc
     6961 gcatagagcc gatgcgtacg cataagccgt cgattacggt cggattgtcg ctgcggccga
     7021 ggattttgtt ggtttccaca ccgcctttcc attcttcttt agattgaccg ttgcccaaat
     7081 ccacgtcaat ccacggaatc aggctgccgg cgagcggtac gccgaagttg gctttcggat
     7141 agtcttcgct gcgcaggaaa tcggacactt tgcggtcgat gtcgagaatc gcgctggaag
     7201 gatcggcaag ctcgtccgct actttggcgt gaatcgcgcc catgccgctg atgagttcgc
     7261 gcatgttttt cgcacccgca ccggaagcgg cttggtaggt catgctggtt gcccattcga
     7321 ccaaatcgtt ttggaacaga ccgcccaaag ccatcagcat cagggaaacg gtacagttgc
     7381 cgccgatgta gtttttcacg ccgtttttga ggccgttgtc gatgacgttg cggttgaccg
     7441 ggtcgaggac gataatcgca tcgtctttca tgcgcaggga tgacgccgcg tcaacccagt
     7501 agccgttcca gccgctgtcg cgcaggggtt ggaagacgga tttggtgtaa tcgccgccct
     7561 gacaggtaac gatgatgtcc atttttgcca attcggcaac atcgttggca tccaacaatg
     7621 ttttggctgc ctgaccgaaa tcaggggctg cgccgccgac gttggaagtg gtaaagaaaa
     7681 acgcttcagg aatgtgggcg aagtcgtttt cttctttcat acgctgcatc aaaaccgaac
     7741 cgaccatacc gcgccagccg acgaaaccta ctttcattgt gttgctcctt gaggaaaaat
     7801 cgttaataaa attatgtatg gcgtaaagga agcgttttaa cgccgtattg ggaaaatgta
     7861 aagttcttta tcgggctttc tttaagtatt ggcgttttcg gtgtcgtggc ggtagaatcg
     7921 gggtcagtta agaacaatta tcaggaacgg atatggaaga aaaaaacgac tacaccgacg
     7981 cggcatccga caaccgcaac gggcaggaaa ttgaagtcgg aattgccggc gcaggcgacc
     8041 ggatacttgc cgcgctgctg aaccaattgt ttaccttttt ggttttgttg gtgccgtttg
     8101 ccggactgat tgctttcgcc atcaagaatg aaggcaggat tggcggcagt gaggaaatat
     8161 tcgggctgct tttgggcatg acttcctttt gggtaggact ggcgggcatt ttggtttata
     8221 ccgtcgttca aatctactat atgagtcggg acgggcagtc attgggtaag aagatcatga
     8281 gaatcagggt gttgaaaacc gacggccgca atccgggttt tgtcggcacg gttttggtac
     8341 gcgaaatcgc atggtcggtt ttggttgcca ttattgccgc cgttatcggt cttgcagtag
     8401 gtgacaacgg agaaaacgcc atcaacctgc tggcattcct tgccaacttt gtcctgctct
     8461 ttatggtcaa acgcgaccgc cgcacgcttt acgacatact ggcggatacg gttgtcgtca
     8521 agctgcccaa ataaacggac acggcaaaat gccgtctgaa agcctttcag acggtatttt
     8581 tatcttcaga accgctcaag catcgtgcag tgaagccgcg tgcagggtgt tttccatcaa
     8641 tgtggcaatc gtcatcggac ccacgccgcc gggaacgggc gtaatcatcg ccgcccgttc
     8701 ttttgccgtt tcaaattcca cgtcgccgca caggctgcca tcgtccaaac ggttgatgcc
     8761 cacatcaata acgaccgcgc caggtttgat ccattcgcct ttgacaaagt tcggaatgcc
     8821 tacgccgacc accaaaatat cggctccggc aacctcgtct gtcagatttt cggttgcgct
     8881 gtggcagacc gttaccgttg cgcgcgccag cagcagttcc aaagcctgcg ggcggccgac
     8941 gatattcgac gcgccgacca cgaccgcttt tttccccttc ggatcaatgc cgtaagcttc
     9001 caaaagcgtc atcacgccct tgggcgtaca cgggcgcatc agcggcattt tgaccgccag
     9061 cctgccgaca ttgtaaggat ggaagccgtc cacgtcctta tccggcgaaa tacgttccaa
     9121 aaccgcctgg ctgtcgaggt gcttcggcag cggtagctga accagaatac cgtccacttc
     9181 ggaatcggca ttcaggcggt cgaccagtgc cagcagttct tcctgcgatg ttgattcggg
     9241 cagctcgtaa gacagtgatt tgatgccgca tttttggcag gcggttttct tgttgcggac
     9301 ataaaccgcg ctggcagggt cgcctccgac caaaaccacg gccaggcaag gcatgtgcag
     9361 attgtcctgt tggcgttgcg ccaccgcttc ggcaaccgcc tgcaggcgtt tttgcgaaac
     9421 ttctttacca ttgatcagtt gtgccgacat atccgtcctt ttgttaaaca ggtagaaaat
     9481 gctgaaaatt atcgcatttt ccgtttgttt ttacagcagg atgccgaaaa taaatttgcc
     9541 ctgaaataaa atacggctgt ttggacggat taaatatttt ttcaaatcaa aaaactatat
     9601 ttttcatctt ggtgtcttga aaataaatat tgacgcatct ttccctgagt tgtatagttc
     9661 gcttcttcaa gtcggggcgt agcgcagccc ggttagcgca tctgctttgg gagcagaggg
     9721 tcgtgagttc gaatcccacc gccccgacca atcaatcagc acgaatcggc atgagcgccc
     9781 gtagctcaac cggatagagc accgaccttc taagtcgggg gttacaggtt cgattcctgt
     9841 cgggcgtgcc aagaatttga ttgaaaaccg tttttatggt ggctgtagct cagttggtag
     9901 agccccggat tgtgattccg gttgtcgtgg gttcgagccc catcagccac cccagataaa
     9961 ggcccgtact tttcggtacg ggtcttttct cgtttgcgcg tactgtccgc gccgccggct
    10021 ttgcctttcg catatgcttc tgtatcatgt aggttttctg tcttttatgg tttttgtcgg
    10081 agactttgat ggttgacgct tggtctgttc ctgattttga atcgaaaatc tgtccgcccg
    10141 aggcgttggc ggcgcgtttg gcgttgttgc cgcgcccgct ggtgtttacc aacggctgtt
    10201 tcgacatcct ccacaggggg cacgttactt atctggcgca ggcgcgttcg atggggggcg
    10261 cgttggtgct ggcgttgaat accgatgctt cggtgcggcg tttgggcaag ggcggcgacc
    10321 gcccgattaa tcctttggag aaccgtgccg ccgttgccgc cgcgttggaa agtgtggatt
    10381 tggtaacgtg gtttgacggg gatacgccgg cggcgttgat tgaggcggtc aaacctgaga
    10441 ttttggtcaa gggcggcgat tgggctgcgg ataagattgt cggtgcggca gaaacgttgg
    10501 cgcgcggcgg tcaggtgttt tcaattccgt ttctgcacca gacttcgaca acgaagactt
    10561 tggcaaaaat ccgtgcggca gagggcggaa aatgacggtt ttgaagcctt cgcactggcg
    10621 ggtgttggcg gagcttgccg acggtttgcc gcaacacgtc tcgcaactgg cgcgtatggc
    10681 ggatatgaag ccgcagcagc tcaacggttt ttggcagcag atgccggcgc acatacgcgg
    10741 gctgttgcgc caacacgacg gctattggcg gctggtgcgc ccattggcgg ttttcgatgc
    10801 cgaaggtttg cgcgagctgg gggaaaggtc gggttttcag acggcattga agcacgagtg
    10861 cgcgtccagc aacgacgaga tactggaatt ggcgcggatt gcgccggaca aggcgcacaa
    10921 aaccatatgt gtgacccacc tgcaaagtaa gggcaggggg cggcaggggc ggaagtggtc
    10981 gcaccgtttg ggcgagtgtc tgatgttcag ttttggctgg gtgtttgacc ggccgcagta
    11041 tgagttgggt tcgctgtcgc ctgttgcggc agtggcgtgc cggcgcgcct tgtcgcgttt
    11101 gggtttgaaa acgcaaatca agtggccaaa cgatttggtc gtcggacgcg acaaattggg
    11161 cggcattctg attgaaacgg tcaggacggg cggcaaaacg gttgccgtgg tcggtatcgg
    11221 catcaatttc gtgctgccca aggaagtgga aaacgccgct tccgtgcaat cgctgtttca
    11281 gacggcatcg cggcggggaa atgccgatgc cgccgtgttg ctggaaacgc tgttggcgga
    11341 acttgatgcg gtgttgttgc aatatgcgcg ggacggattt gcgccttttg tggcggaata
    11401 tcaggctgcc aaccgcgacc acggcaaggc ggtattgctg ttgcgcgacg gcgaaaccgt
    11461 gttcgaaggc acggttaaag gcgtggacga acaaggcgtt ctgcacttgg aaacggcaga
    11521 gggcaaacag acggtcgtca gcggcgaaat cagcctgcgg cccgacaaca ggccggtttc
    11581 cgtgccgaag cggcgggatt cggaacgttt tctgctgttg gacggcggca acagccggct
    11641 caagtgggcg tgggtggaaa acggcacgtt cgcaaccgtc ggtagcgcgc cgtaccgcga
    11701 tttgtcgcct ttgggcgcgg agtgggcgga aaaggcggat ggaaatgtcc gcatcgtcgg
    11761 ttgcgccgtg tgcggagaat tcaaaaaggc acaagtgcag gaacagctcg cccgaaaaat
    11821 cgagtggctg ccgtcttccg cacaggcttt gggcatacgc aaccactacc gccaccccga
    11881 agaacacggt tccgaccgct ggttcaacgc cttgggcagc cgccgcttca gccgcaacgc
    11941 ctgcgtcgtc gtcagttgcg gcacggcggt aacggttgac gcgctcaccg atgacggaca
    12001 ttatctcggg ggaaccatca tgcccggttt ccacctgatg aaagaatcgc tcgccgtccg
    12061 aaccgccaac ctcaaccggc acgccggtaa gcgttatcct ttcccgacca caacgggcaa
    12121 tgccgtcgcc agcggcatga tggatgcggt ttgcggctcg gttatgatga tgcacgggcg
    12181 tttgaaagaa aaaaccgggg cgggcaagcc tgtcgatgtc atcattaccg gcggcggcgc
    12241 ggcaaaagtt gccgaagccc tgccgcctgc atttttggcg gaaaataccg tgcgcgtggc
    12301 ggacaacctc gtcattcacg ggctgctgaa cctgattgcc gccgaaggcg gggaatcgga
    12361 acatacttaa aaaatacacc ctttttcaac acggggcggt atccgtccga atccgtccgg
    12421 attctgccgc ccgacagact gccgcccagg cgggcggggt ttcaaacatt gaggaaattc
    12481 taatgaaatg gctcttcacc cttttggcgg tactcaatat tgccgtattc ggcggcacgg
    12541 tgggctataa attggccgtt aaagcgacgg gcggcgtgcc ggaaaaccgg gccgtcgaaa
    12601 acacgccccc cgcaacgccc gccgccgaca atgcggcggc atccgtggag gatacggcgg
    12661 cactgctcaa accgggcgac atcctgaccg aagagcaggc ggagcagttg cgcttgaaga
    12721 aagaagcgga acagaaaaaa ctgagggaga aaaaacagcg tgaagaaaaa gcccgccgcg
    12781 aaaaactcgc cgccgaaaag gcgcaggcgg aacgcgaaaa cggcgcggcg gatgccttat
    12841 gcgccgcgca ggcaagcctc acgatggacg aagatgacta ccaccgcatc aaaggacttt
    12901 tgggcaaatg gtcgcacgtt gccagcagga gcgtcgaaaa acgcaccgcc aaagccaaac
    12961 ctgccgacaa aacctaccgc gtcgtcctgc ccgtttccgc cgatgccgaa aatcaggcgg
    13021 cggagctgtc tgccaaaggt ttcaacccca taccgtttga cggcgcattg agtttgggtg
    13081 tcggcaacag ccgggaaaac gcccaagccc tgcaaaaccg gcttgccggc gccggattcg
    13141 gcggggcgca tattgtcgaa cactttgccg aagccgacag gcaggacgat tctttgagcg
    13201 tgtcgcgtat gacggttttg tttaccggcg tgaatgccgc cgatgcggac gaaatccgta
    13261 aaatcacgtc cctatacggc aaactgaacc tcaagtcttg caaataggcg cgaaagccgg
    13321 acgataaacg ccaaacccac cgcgaaggtg ggtttggcgt ttcggttttg agtatctgat
    13381 atataaaaag ggaatgtatg tctcgtatat gccgactcca agtgtgaaag tgatgatggg
    13441 gaaatatggt gtttaatggt tcggaacgga cggcttccga gtggttttga ttatacgcta
    13501 ataataataa ttatcaataa aaatttaaaa ataaatgcag attttttgta atacgatgaa
    13561 aaataaacaa attaacctgt ccgccgatgt ttgtgattcg ggtcggcaaa ctttcattgt
    13621 gtcggacaaa tcggggtcgg cagtcatagc tgagggcttc gtgcctgacg gaaataaggt
    13681 tgcccgtgtt tgaacatccg tccgattggg cgcggacagg gttgtcattc gggggtgttt
    13741 gagcgtggat tcggggatgc gaaagcggta aaaccgttgc ggatgaaggc ttacggacgg
    13801 ctttaggtat ttttaagtgt attcaaacaa caacaaaacg cttcttctat tgccgtattt
    13861 agggcttgcg cggcagcctg cacaccgtgg aggccgagtg tttcgttggt tgcggcatcg
    13921 ggacgggcgg caaggttttc cggccggttt ttccattttt tccgacaaag ccgtgatgcc
    13981 ggggaatggc ggtttgtccg tttcggaccg ccgccgtttt gagccgcgat gccgtctgaa
    14041 ccgttcagac ggcatttttg ccgccttttt caatattccg ccgaatgcca aaacggctgt
    14101 ccgactgtcg gcgtgctggc ttgcgctttg tcgcgtgctt cgaccggttc ggcttcaaat
    14161 gccagccgtc cggattcgac ggcgagtgcg aaggcgcgtg ccatattgac cggatcgccg
    14221 ctgcgggaaa cggcagtatt caaaagcacg ccgtcaaaac cccattccat cacttgtgcc
    14281 gcctgtgagg gcaaacccaa gcccgcgtcg ataatcagcg gcgtgtcggg caggcgttcg
    14341 cgcaggacgt tcaacgcgta ggcgtgaacc gcgcccaaac ccgtgccgat cggagccgcc
    14401 cacggcatta acgcctgaca gcccgcgtcg agcaggcggc ggcaggcaat caggtcttcg
    14461 gtgcaataag gcagcacttt gaagccgtct ttaatcagga tttccgccgc ttcgacaagt
    14521 tggaacacat ccggctgcaa ggtgtcgtca tctccgatga gttccaattt tatccaatcg
    14581 gtttcaaaca cttcgcgcgc catttgcgcc gtcgttaccg cttcctgcac gctttggcag
    14641 cctgccgtgt tcggcaggac gggaacgccg gtttcttgaa gcagcgacca aaacccctga
    14701 ccgtgcgcct cgccgccgct tcccgcgcgg cgcagcgaga cggtaatcat cgcaggctgg
    14761 gcggtttgga tggattgttt gaggatttcg ggggtcgggt aggcggctgt gccgagcagc
    14821 agccgcgaag ggaaagtttc gctgtacagg gtgagcataa tgggttcctt tgtatagtgg
    14881 attaacaaaa accagtacgg cgttgcctcg tcttagctca aagaaaacga ttctctaagg
    14941 tgctgaagca ctaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct
    15001 tgtcctgatt taaagttaat ccaccataac gttgttttgg ggacaggggt cgtctgaaaa
    15061 ggcaaaaccg cctagccgcc gaccaccggc cgcacgatat cgattttgtc gttttcgttt
    15121 aaaaccgttt ccgcatacgc gcctttgggg acgaaaacgg tgttgaccgc cacggcaaag
    15181 ggcttttgcg gcgcggtttg ggcgatgagg tcggcaacgc ttgtgccgtg aagttcggcg
    15241 ggtccgccgt ttaagatgat gttcatggtt tctcgatgtt tctgtattta tagtggatta
    15301 acaaaaacca gtacggcgtt gcctc
//
LOCUS       DAWFIT010000045        15233 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFIT010000045 DAWFIT010000000
VERSION     DAWFIT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.045"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            91..813
                     /locus_tag="WGQ85_001283"
                     /pseudo
     CDS             91..813
                     /locus_tag="WGQ85_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            complement(1020..1502)
                     /locus_tag="WGQ85_001284"
     CDS             complement(1020..1502)
                     /locus_tag="WGQ85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0750702.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(1655..1816)
                     /locus_tag="WGQ85_001285"
     CDS             complement(1655..1816)
                     /locus_tag="WGQ85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750703.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(1890..2351)
                     /gene="recX"
                     /locus_tag="WGQ85_001286"
     CDS             complement(1890..2351)
                     /gene="recX"
                     /locus_tag="WGQ85_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0750704.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(2419..3126)
                     /locus_tag="WGQ85_001287"
     CDS             complement(2419..3126)
                     /locus_tag="WGQ85_001287"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0750705.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            3533..4798
                     /locus_tag="WGQ85_001288"
     CDS             3533..4798
                     /locus_tag="WGQ85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0750706.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(4856..5662)
                     /locus_tag="WGQ85_001289"
     CDS             complement(4856..5662)
                     /locus_tag="WGQ85_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0750707.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            6007..6216
                     /locus_tag="WGQ85_001290"
     CDS             6007..6216
                     /locus_tag="WGQ85_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0750708.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            6305..7519
                     /locus_tag="WGQ85_001291"
     CDS             6305..7519
                     /locus_tag="WGQ85_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0750709.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(7606..10185)
                     /gene="clpB"
                     /locus_tag="WGQ85_001292"
     CDS             complement(7606..10185)
                     /gene="clpB"
                     /locus_tag="WGQ85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0750710.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALIQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            10446..11456
                     /gene="trpS"
                     /locus_tag="WGQ85_001293"
     CDS             10446..11456
                     /gene="trpS"
                     /locus_tag="WGQ85_001293"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0750711.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            11462..11803
                     /locus_tag="WGQ85_001294"
     CDS             11462..11803
                     /locus_tag="WGQ85_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750712.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHTNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            12124..12489
                     /locus_tag="WGQ85_001295"
     CDS             12124..12489
                     /locus_tag="WGQ85_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750713.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(12698..14206)
                     /gene="ppx"
                     /locus_tag="WGQ85_001296"
     CDS             complement(12698..14206)
                     /gene="ppx"
                     /locus_tag="WGQ85_001296"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0750714.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(14322..15054)
                     /locus_tag="WGQ85_001297"
                     /pseudo
     CDS             complement(14322..15054)
                     /locus_tag="WGQ85_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3461 a         3812 c         4166 g         3794 t
ORIGIN      
        1 gtcaaaccgg caggatatgc cgcaccgaaa gccgcagccg taaaaagcag gcccgccgta
       61 ccggttgccg cgcaaccgcc cgtacagtcc gcacccgtcg acatcaacgc ggcgacgcat
      121 actattgtgc gcggcgacac ggtgtacaac atttccaaac gctaccatat ctctcaagac
      181 gatttccgtg cgtggaacgg catgaccgac aatatgttga gcatcggtca gattgttaaa
      241 gtcaaaccgg caggatatgc cgcaccgaaa acctcagccg tagaaagcag gcccgccgta
      301 ccggctgccg tgcaaacccc tgtgaaaccc gccgcgcaac cgcctgtgca gtccgcgccg
      361 caacctgccg cgcccgctgc ggaaaataaa gcggttcccg cgcccgcccc gcaatctcct
      421 gccgcttcgc cttccggaac gcgttcggtc ggcggcattg tttggcagcg tccgacgcaa
      481 ggtaaagtgg ttgccgattt cggcggcaac aacaagggtg tcgatattgc aggaaatgcg
      541 ggacaacccg ttttggcggc ggctgacggc aaagtggttt atgccggttc cggtttgagg
      601 ggatacggaa acttggtcat catccagcat aattcttcct tcctgaccgc atacgggcac
      661 aaccaaaaat tactggtcgg cgaaggtcag caggtcaaac gcggtcagca ggttgccttg
      721 atgggcaata ccgacgcttc tagaacgcag cttcatttcg aggtgcggca aaacggcaaa
      781 ccagttaatc cgaacagcta tatcgcgttc tgactttctg cctgaaatca agttggaaat
      841 gccgtctgaa aggttttcag acggcatttt tgtttttggc ggctgtgttt tcaaatcatt
      901 cggaagggac ggggcatata aatttgtttg actgtgtttg ggtatttgct tgatttcatc
      961 cgtttgcagt agaaacggat ggcgcgatgt gcctgtcttt cagacggcat agtccgccgt
     1021 cagcagccgc aggacatgtc ttcagaggct tgcaggctga tgtctctgcg ttttttggct
     1081 ttttcgtagc ggcagcgttg gcggtcggtc aggatttcca gcggagggac agggacgggt
     1141 ttgccgtctt tgaccgcaac catcgtgaag tagcagctgt tggtatggcg gatttcgccg
     1201 gtgcggatgt tttgtgcttc gacacggatg ccgatttcca tcgaggtgcg tcccgtgtag
     1261 ttgacagcgg cgtaaaaggt aaccaaatcg ccgatgtgga tgggttcttt aaacaggact
     1321 ttgtcaaccg acagggtaac gcaataattg ccgctgtaac ggctggcgca ggaataggcg
     1381 acttggtcga gcaggagcag gagttcgccg ccgtgtacgt tgccgctgaa attggcggtg
     1441 tccggcatca tcagttcgga cataatgagt tcgtgggaag gcagttggcg ttgttgtgtc
     1501 atagtgtacc tgttttaaga aagggtgttt tgaaatgggt gcggggaaat tttagggaat
     1561 ggggaagtgg aattcaagct aaaaaaagct atgatgggat agtctgccga aatggaccgc
     1621 cgatgccgtc tgaagccttc agacggcatt ttgtttagga aagccaaaaa cggaagacca
     1681 ggaaaaacac gagtgccaat acgcccgccc aaacgacgag catgaatcca gcaactgccc
     1741 gtcccatacg gtaaaccggg tttttgcgtt cgtcttcgat gggggaggtg tggtgttttt
     1801 cgggttgtcc ggacattttg ttctcaatcg gaaaagttgg aaacgggcgt aaggttatgt
     1861 tgccgtgcaa aaagattcaa gaaggaaggt cagcagccgt cttcccaatt ttcgtcccaa
     1921 gcgtgtttca atgccgtctg aacggtatcg gcatcgaatc cgcgataggc gaggaaacgc
     1981 gcctgttttt gtttttcttt gaggtcggcg gcggggtgtt tgaatttttt acgcaacacg
     2041 gctatggcgg ccagtttttc gcttgagcgg tcgggaagca ggttgcggct ggtttcttca
     2101 tctatgccct gttgcgccaa agcctgtttc agcctcaatg aaccgtgttt gcggcttttg
     2161 ctgcggatat aggcttcggc atagcgcaaa tccgactgcc agttgcgttc ggcaaattcg
     2221 tttaacacgt tttccaactc ctcttcgctt tcggcgtgcg gtgcaagttt gcgtttcaga
     2281 ccgatgcggc tgagttcttg gcgcgagagg atgtccatcg cgcgggcgcg tagggatttt
     2341 tgcggtttca tggcggcggt ttcggcgtgg ggcaggtttc agacggcata gcggcgcaaa
     2401 ccggagccgt ccgaatgcct actcttcttt gtttttctga ggttgcaggt tttcgtaaat
     2461 ttcgggcagc gagccgataa tccagtcggg gcgggtcgca tcgtcttgcg agagcagcgt
     2521 catatcgccg taaccgaagg taacgccgac gctcaggcag ccggcggctt tggcggcgat
     2581 gatgtcgttg cgcgagtcgc cgaccataac catgtttgca acatcgatac ccaaaacttc
     2641 ggcggcgtgc cgcagcggca gggggctggg ttttttctcg ggcaggctgt cgccgccgag
     2701 tatcaggctg aagtagtcgg caagtccgag ctgtttcaac agttcgacgg caaggatttc
     2761 gtttttattg gtaatgactg ccagcgggat gcccaaagat ttgagcaatg ccagcccggt
     2821 tgcggtttcg ggataggggc gggtgaagac gctcaaatgg tcgcggtagt atttcatata
     2881 ggatacgaaa cctttttccc acagttcgga atcggcttcg cggtcgcggt cgttggtgag
     2941 gacgcggtga accagtttgc cgatgccgtc gcccacatag ctttcgacca ctttggcggg
     3001 cagcggtttc ataccgagtt gttccaacat cgcttctgcg gcggcggcaa ggtcggggac
     3061 ggaatcgcac agtgtgccgt ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc
     3121 attcataatc tgatttcctg tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg
     3181 cttctaatgt atagattgtt aacaatcgca acaggttttg caggcggtgt tttcggattt
     3241 cggggcggaa tcgggcaagg gtttgaaatc ccagcgatgt tccaattaaa tttttaaaaa
     3301 ataaaaggat gcctatatct ttatttaatt tgacatttat ttttcggatg cgcgcgctgc
     3361 tttttcaggc ggagtccggg ctgattccgt gcggcttgaa aaataaaatc atatatagat
     3421 taatcaaatc gggcttatgc cgactacacg ctattacatt ttgtcgggaa attggttata
     3481 gtaatgagcg aaatgcacgc ccctgaaaat ctttttgtga aaaggaagca aaatgtctga
     3541 agcccttgca aaagaaaccc tcaatccgtt tgaaatcgcg cgcaaacagg tcaaaaccgc
     3601 ctgcgaccgg ctgaaaaccg atccggccgt ttatgaaatc ctgaagagtc cgacccgcgt
     3661 gttggaagtc aatttccccg tcaaactcga cgacggcacg gtcaaaacct tcacgggcta
     3721 ccgttcgcaa cacaacaacg ccgtcggccc ctataaaggc ggcgtgcgct tccatcccgg
     3781 tgtgaatctg gacgaagtca aagccttgtc aatttggatg accatcaaat gctgcgttgc
     3841 gggtattcct tatggcggcg gcaaaggcgg cattaccttg gatccgcgcg attattccga
     3901 ggcggaactg gaacgcatcg cccgcgctta ttccgaagcc atttctccgc tgatcggcga
     3961 gaaaatcgat attcccgccc ccgatgtgaa caccaacggc aaaatcatgt cttggatggt
     4021 tgatgcctat gaaaacgtgg tgaaacattc tgcaccgggc gtatttaccg gtaaaccggt
     4081 tgagttcggc ggctctttgg cgcgtaccga agccacgggt tacggcgtga acctcgccgc
     4141 cgtccaagcc ttggaaaaac tgggcaaaga cgtgaagggt gccacttacg ccattcaagg
     4201 tttcggtaat gtgggttacc acacaggtta ttacgcgcat caatccggcg cgaaagttgt
     4261 tgcggtttct actgtcgatg ttgccattta caacgaaaac ggtttggata tggaagccct
     4321 gttcaaagag ttccaagaaa aaggcttcat caccaacgaa gccggttacg gcaaggaaat
     4381 taccaacgcc gaacttttgg ctttggatgt ggacgtactc gccccttgcg cgctggaaaa
     4441 tcaactgact tctgaaaacg ccggtaaagt ccgcgcgaaa atcgtggtcg aaggcgcgaa
     4501 cggcccgact acgcccgaag ccgacgtgat cctgcgtcaa aacggcgtat tggtcgtgcc
     4561 cgatattttg gcgaactgcg gcggcgtggt ggtatcttat tttgaatggg tacaaaatct
     4621 gcaaggctat tattgggagt ttgacgaagt tcaggaaaaa gaaaccgtcg tcctgcgccg
     4681 tgcgttccgc gatatatgga acttggcgca agaatacgat gtcgatttgc gaaccgcgtc
     4741 ttacatgatg agtatccgcc gtgttgaaaa agcgatgaag ctgcgcggtt ggtattgatg
     4801 tttgaacaaa aaatgccgtc tgaagccttc agacggcatt tttgtgcagg cgggattaat
     4861 ggtgcgaatg gccgatttgg aaggttaaag tcgagtagtt cgcctgtttt tggcacacgc
     4921 tttgatcggg gaagtcggct ttgtgttcga cattggcttt ccagaagcct tggcgcaagg
     4981 ggatgatgtc cacttcgcct ttgtcgtctg tgctgtcgga gaaagcctgt gcttcggttt
     5041 tgtgcgtttt gctgcggtcg ctggtgtcga agccgtcaaa ggtggcggta acggtggcat
     5101 tgggcagcgg ttcgccacgg aacagaacgc ggactttgaa gcgttcgcct acgtgaatgt
     5161 tggcgggatt gtccagcggg acgatttcca agttttgtcc gaccggtttg gtgatgatgg
     5221 cggtgtccgc gctttcgtgt ccgacgttga cgatgttttt gccgaacatt cgggtttgtt
     5281 cgcaatagct tgcgtcaggc atttctttga tgcccgcctg tttccagcct gctttgtttt
     5341 ttgaccagaa agtaggctga tattcggcga tgacgaggta actgccgtcc ttaacgggac
     5401 ggttgcttcg gtactggtag ttgtatgtgc cgcgttgaat catgttttcc ttgccttttt
     5461 cggtaaccag ctgcatcggt ttgctgaaga tgtgcaggcg gtctttggcg atgggttcga
     5521 gttcgggaaa ttcgccgtag cccaagtcgg ctttaaggta ttcgccgccg tgcgtgtggg
     5581 cggtttcgac ccagacgcgg tgggcgtggg cggatgtggc aaatagggcg gaaacggcga
     5641 gcagtgtcaa tgttttcttc atggtgcagc ctttgtctcg tgtgggtcaa agattttgtt
     5701 atgttataac aaaataggta tcgtgtgaag acggatttgc tgtctgaaaa ctgtcttttt
     5761 gtatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa agagaacgat
     5821 tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact gtatgcggct
     5881 tcgtcgcctt gtcctgattt ttgttaatcc gctatatcga tttgaatttt tcagaaaatg
     5941 aagcggacgt ttgggcggag gcaacttgtt tgataagata gcaatatttc aaaacggaga
     6001 aagatcatgc cttatgtcaa tattaaagta accggcggca aggaagcacc gactgccgcg
     6061 caaaaagcgg aactgatcgg cggcgtaacc gaattgctgg cacgcgtgct gggcaaaaat
     6121 cccgaaacaa cggttgtcgt gattgatgaa gtggataccg ataactgggg aataggcggc
     6181 aaaagcgtca gcgaacggcg caaagagggc aggtaaaaag cctgaaaatc tcggtttgat
     6241 gctttaaatt ccgcgtgaaa aagagtacat tcccacccat tgcccaaaat ttacggaaca
     6301 catcatggat aaatttccca agtctgcaaa gctcgatcac gtctgttacg acatacgcgg
     6361 gccggttcac aaaaaagccc ttcagttgga agaggagggc aataaaatcc ttaaactcaa
     6421 tatcggcaac cctgcgccgt tcggctttga agcccctgat gaaatcttgg tcgatgtcat
     6481 ccgcaacctg ccgacttcgc aaggctattg cgattctaaa gggctgtatt ccgcccgcaa
     6541 agccattgtt cactactatc agaccaaagg tttgcgcgat attacggttg atgatgtcta
     6601 tatcggcaac ggcgtgtccg agctgattac gatgtctatg caggcattgc tcaacgacgg
     6661 cgacgaaatc ctgattcccg cgcccgacta ccctttgtgg acggcggcgg caacgcttgc
     6721 gggcggtacg gtacgccatt atctgtgcga cgaagaaaac ggctggttcc ccaaccttgc
     6781 cgatatggaa gccaaaatca cgcccaaaac caaagccatc gtcgtcatca atcccaataa
     6841 tccgacaggc gcggtgtaca gcagggaaat cctgttggaa atcgccgaac tggcgcgcaa
     6901 gcacggtttg attattttcg ccgacgagat ttacgataaa atcctttatg acggcgcggt
     6961 tcaccaccac atcgccgcgc ttgcccccga ccttttgacg gtaacgttca acggtttgtc
     7021 caaatcctac cgcgtcgccg gcttccgcca aggctggatg gtgctcaacg ggccgaaaca
     7081 tcacgcaaaa ggttacatcg agggtttgga tatgctctcg tctatgcgcc tgtgtgccaa
     7141 tacgccgatg cagcacgcga ttcagacggc attgggcggc tatcagagca tcaacgaatt
     7201 tattttgccc ggcgggcggc ttttggaaca gcgcaacagg gcgtgggaac tggtcagcca
     7261 aatccccggc gtgtcctgcg tcaaaccgat gggtgcgatg tatatgttcc cgaaaatcga
     7321 taccgaaatg taccgcatcc gcgatgacat gaaattcgtt tacgatttgc tggtgcgcga
     7381 aaaagtcttg ctggtacagg gaacggggtt taattggatc aagcccgacc atttccgcat
     7441 tgttacgctg ccttacgtcc atcagattga agaggcgatg ggcaggctgg caagattcct
     7501 gcaaacctac cgccaataag gggacgggct gtctgccgag gataaaaaat gccgtctgaa
     7561 acggagattc ccgtttcaga cggcattttt aacagcagga acgaatcagg caaatttcag
     7621 tctgtcgccg tcggcttcca ccctgatttc gctttcgggc gcatagtttc cggcaagcag
     7681 ggcttttgcc agcgggtttt cgatttccga ctggatggcg cgtttgagcg gacgtgcgcc
     7741 gtaaatcggg tcgaaaccgg ctttggcgat gatgtccagt gcggcatcgg aaacagccag
     7801 gcgcaggttt tgtttttcca aacgtttttc caagcctttg agctggattt tcgcaatgtt
     7861 gcggatatta tcctgatcca gtccgtggaa cacgaccact tcgtcgatgc ggttgatcat
     7921 ttcggggcgg aaatgttctt tcacatcttc catcacaact tcttttaccg cttcatagtc
     7981 ttgagtaccc atttgttgga tatgttggct accgatgttg gaagtcatca caatcacggt
     8041 gtttttaaaa tctaccgttc tgccctgtcc gtcggtcaaa cgaccgtcat ccaatacttg
     8101 cagcaggatg ttgaacacat cgggatgggc tttttccact tcgtccaaaa gaattacgct
     8161 gtacggtttg cggcggactt gttcggtcag gtagccgcct tcttcgtagc cgacatagcc
     8221 tggaggcgcg ccgatgaggc gggcaacggc gtgtttttcc atatattcgg acatatcgat
     8281 acgaatcaga tgatcttcac tgtcaaacag gaagcctgcc aatgctttgc acaactcggt
     8341 tttacccacg ccggtcgggc ccaagaacag gaagctgccg taaggtttat tcgggtcggc
     8401 aagaccggag cggctgcggc ggatggcgtc ggacacggca cgcacggctt cgtcttgacc
     8461 gactacgcgg cggtgcaaca cttcttccat tttcagcaac ttgtcgcgtt cgccttccat
     8521 catttttgac acaggaatgc cggtcatacg ggaaaccact tctgcgattt cctctgcgcc
     8581 gacattatta cgcaagagtt tgtttgccgg ttttgtgctg tccgtatctg cccgttcggc
     8641 ggctgcacgc tgtttttcca aatgctccaa atcttcatac atcaattttg aagccagtgc
     8701 caaatcgcct tgccgttttg cctgttcgat tttaattttg acttcgtcaa tttgtttctt
     8761 aatattagca gcaccgtctg aaattgcttt ttcggctttc cagatttcgt ctaaatcggc
     8821 gtattctttt tgcagaccgt tgatttcctc gtctatcagt tccaaacgtt ttttgctggc
     8881 atcgtctttt tctttttcaa cgtgcgcctt ttccatccga agctgaatta gacggcggtc
     8941 gattttgtcc attgcttccg gcttggtttc tttttccatc ttgacacggc tggcggcttc
     9001 gtcaatcaaa tcaatcgctt tatcaggcag gaagcggtcg gtaatataac ggtcgctcaa
     9061 ctccgctgcg gcaacgatag cagggtcggt aatatcgata ccgtggtgga tttcataacg
     9121 ctcctgtaaa ccgcgcaaga tagcgatggt gtcttccaca ctgggttcgc cgaccaatac
     9181 tttttggaaa cggcgttcga gtgccgcatc tttttcgatg tattggcggt attcgtccaa
     9241 ggtggtcgcg ccgatgcagt gcaattcgcc gcgtgccaaa gccggtttca acatattgcc
     9301 cgcgtccatt gcgccgtcag ttttgcctgc gccgaccaaa gtatggattt catcgatgaa
     9361 aatcagggtg ttgccgtcgt ctttcgccaa atcgttcaat acgcctttca agcgttcttc
     9421 aaattcgccg cggtatttcg cgccggcaat caaagccgcc aaatccaaaa ccagcaggcg
     9481 tttgttacgc agggattcag gtacttcgcc gttgacgata cgttgcgcca agccttcaac
     9541 aatagcggtt ttacccacac ccggctcacc aatcagcaca gggttgtttt tggtacggcg
     9601 ttgcaatacc tgaatcgcgc ggcggatttc gtcgtcgcgg ccgataacgg ggtcaagttt
     9661 gccgtcgcgg gcgcgttgag tcaggtcaag tgtgtatttt ttcaaagcat cgcgttggtc
     9721 ttcggcattc gcatcattca cgttttgtcc tcctcgtacc gcgtcaatcg cggcattgat
     9781 attttgttcg gtcgcgccgg cttctttcaa aattttgccg gtcgcatcgt tctgctgaat
     9841 caaggcaagc aggaaaagtt cgctggcgat ataggcatcg ccacgtttgg ttgctgcttt
     9901 gtccatcagg ttcaacaccg cctgcaattc gcggctgggc agaatatcgc cgccctgacc
     9961 ggacactttc ggcaggctgc tcaaatgctg ctgcaaacgc tgtttcacct gcggcacgtt
    10021 cacgcccgaa tgcgccagca acgcggcggc tccgctgttt tggtcgtcaa gcagggcttt
    10081 taacacaaag cctgcttcta gatagctgcc gtccgcagcc aacgccaaac tctgagcttc
    10141 tgcaagggct tgttggaatt tggcggttaa tttgtcgtat cgcatttttg tttccttttc
    10201 aaaatgtccg ctgtcgaagc ctatatgtgc ataattgtgg ataactcaag ttctgttttc
    10261 tgtttttcta tatttaattc gatatatcat tgaatttaaa gtatataaaa atgtataata
    10321 atgtgtataa ctatatcttc ttaatatgga aaagtctgtt gtcggctgga tgtaggtggc
    10381 aaatcgggta taatcggcgc atctttttcc ctttcagacg gcattgatgc cgcaaggaca
    10441 tttttatgag caaaaaacga gttctgaccg gcgtaaccac caccggcatc ccgcatctgg
    10501 gcaactacgt cggtgctatc cgccccgccg tccgcgcggc gcaaaaccct gataccgaat
    10561 ccttcctctt cctcgccgat taccacggta tcatcaaatg ccacgagccg gagatgattc
    10621 accaatccac ccaagccgtt gccgccactt ggcttgcctg cggactcgac cccgagcgca
    10681 ccaccttcta ccgccaaagc gacattcccg aagtgatgga attgaactgg attctgacct
    10741 gcatcaccgc caagggtttg atgaaccgcg cccacgccta caaagccgcc gtgcaggcaa
    10801 atgcggaaaa cgggcaggaa gaccccgatt tcggcgtgga aatgggtttg ttcagttatc
    10861 cgattctgat gactgccgat attctgatgt tcaacgccaa cgaagtcccc gtcgggcgcg
    10921 accaaatcca acacgtcgaa atggcgcgcg acatcgccgg ccgcttcaat caccgcttcc
    10981 gagaactctt caccctgccc gaagtgaaaa tcgacgaaaa cgtcgaactc ttggtcggtt
    11041 tggacggacg caaaatgtcc aaatcctacg gcaacaccat tccgctttgg gaaaacgaca
    11101 aaaaaaccca aaaatcggtc aacaaaatca tcaccaatat gaaagagccg ggcgagccga
    11161 aacagcccga cgaaagccca ttgtttgaaa tctacaaagc cttctccacg ccgtctgaaa
    11221 cggtggaatt tacgaaaatg cttgccgacg gcctggcttg gggcgaggca aaaaaacttt
    11281 tggcggcgaa aatcaacgcc gaactcgccg aaccgcgcga acgctacaac gagctgaccg
    11341 ccgacccttc gcaaatcgaa gagattttac aggcaggtgc gcaaaaagcg cgtaaagaag
    11401 cacgcgaatt gttggacaaa gtacgcgatg cggtcggcat ccgcccgttg aaataaaccc
    11461 gatgccgtcc gaacctctta aaggcttgcc ggaacaaaac gtcgtccggc tgatcggcaa
    11521 gcataccaac gacttggaag ccgtcgtcgg caaatgtatg gaaaccgacg gaaagggcgc
    11581 gccttcgggc tgggcggcaa acggcgtgtg ccataccttg tttgccaaac tggtaggcaa
    11641 tatcgccgaa gacggcggca aactgacgga ttacctgatt tcgcattccg ccctgcaacc
    11701 ctatcaggca ggcaaaagcg gctatgccgc agtgcagaac ggacgctatg tgctggaaat
    11761 cgacagcgag ggggcgtttt atttccgccg ccgccattat tgaggtattc ggacatcccg
    11821 gaatatattt gggtttttca aaccctgcag gaaaaagtcc gcaccgacga gaaacgcaaa
    11881 aggaggggga tatgtgttac aattttccga actgttttca taaaatagtt ttcggacgtg
    11941 tttcaatatg gcattgatgc cgccttattg ttcgagaaaa aaaaccttta tatttaaata
    12001 taatgggttt taactaaacg ggaaaccgtt ttctctccgg ccgatgggca aaatcagccg
    12061 attgatggaa cacggtccga tttttaagca aaaccttaat caacaactca agaaggataa
    12121 gacatgaaaa aattattgat tgccgcaatg atggcggctg ccttggcagc ttgttcgcaa
    12181 gaagccaaac aggaggttaa ggaagcggtt caagccgttg agtccgatgt taaagacact
    12241 gcggcttctg ccgccgagtc tgccgcttct gccgtcgaag aagcgaaaga ccaagtcaaa
    12301 gatgctgcgg ctgatgcaaa ggcaagtgcc gaggaagctg taactgaagc caaagaagct
    12361 gtaactgaag ccaaagaagc tgtaactgaa gccaaagaag ctgtaactga agcagctaaa
    12421 gatactttga acaaagctgc cgacgcgact caggaagcgg cagacaaaat gaaagacgct
    12481 gccaaataat ttgttgcttt ggcaaaaatg acgagatgcc gcctgccggc aacccaaacg
    12541 aaaccgcctg aagattttca ggcggttttt gagttgtatg ccccttgttt tttacgcctg
    12601 cataaccgtt ccgagcagat gtgccgcgca tatcgggatg ttgcggacag ggaaatgccg
    12661 tctgaacatc attcagacgg catttgcccc gcaaggttca gacggcctcg actttgaacg
    12721 gcatattgat tttttgccat tggacgcttt catattccaa tgcgtcggca atcaggggat
    12781 ggcgttccag ccattccctg tcaatacgca ggatgaagcc gcaactttcc gtgtccgtac
    12841 gcaactgcat atttttcggg aaagacaggt cttggcgcga acggcagaac agggcggcaa
    12901 ggcgcagcga caaaacggca taccacaaca tttcgttggt gccgataatg ccgcccattt
    12961 ttttcatatc gccgcgatga ccgatgacga gctgggcgag tatggtctgt tctttgcgtg
    13021 agaaacccgg catatcggcg ttttcgagga tgtaggcgga atgcttgtga tagccggtgt
    13081 gggcgatgtc caaaccgatt tcgtgcagcg cggcggcgcg tccgagatac tgttgccaca
    13141 aggcaagctc ttgaactgta acgtttttag cgtggcagag gctgtccata aaggtttgcg
    13201 cggtctcggc ggtgcgtttc gcctgattga ggctgacgtg gtagcggtgt tggaactcgg
    13261 caaccgtttg tccgcgcata tcttcgttta aaccgcgccc gatcaaatcg taaaacacgc
    13321 cgtcgcgcag ggcggcttcg gttacggtca tcctgtcgag tttcatttcc tcaaacgccg
    13381 ccatcatcac ggcaagtccg ccggcaaaaa cttcgatgcg ttccggtttc aggttttcaa
    13441 atttggcttt tttgaccgaa ccggcttcga tgatgcgttc ggagagggcg cgcatacctt
    13501 tgtaggtaat gtccgcctct tggggcattt cggcggcaag cacgtcgcgg atggattttg
    13561 ccgaacccga tgtgccgacg gcgaaatccc agccttcgcg cctcatattt ttgctgatgc
    13621 gctggatttc gttgcgggcg gcggaaatgg cggcttggaa gtctttggcg gtgattttgt
    13681 tttggaagaa gcgcaggctg taggttacgc agcccaaggg caggctttcg gtaatgtcgg
    13741 gattcagcgt cgaaccgatg acaaattctg tcgaaccgcc cccgatgtcg ataaccagca
    13801 ttttgccgcc gccggggggg agggtgtgga tcacgccggt ataaatcagc cgtgcctctt
    13861 cgcgcccggc gatgatttcg atggggaaac ccagtgccgc ttcggctttg ggaaggaaat
    13921 ctgcgatgtt tttggcgaca cggaatgtat tggttgccac ggcgcgtacc tgttcagggc
    13981 ggaagccgcg caggcgttcg ccgaattttg ccagacagtc caaagcctgt tcttgggaag
    14041 cggcactcag gtttttctgt tcgtccagtc cggcggcgaa gcgcaccatc tgtttgaacg
    14101 aatcgatgac tttcaactgt ccgttgttgt tttcgcaaat ctggaggcgg aaactgttag
    14161 aacccaaatc gacggaggca aggacgtttg cgggggtggt ggtcatggcg gataccggtg
    14221 ggggaaaaac gcaatgttac tctgacggcg caggcgttga caataaatga tgcggcggtt
    14281 tttgattctg cccacggatg ttgccgacgg catttttgcg cttatttgaa atccttttcc
    14341 acgctcatga aaatctgcgt gtttttgcgt gtgtaaaaac ttttcatatt gctgtcgatt
    14401 ttcagatagc ggaaattgag ttgcggcgta aagcccttcc aagagatttt gtcgtgccac
    14461 aacgacaggt ttgcctgata ttcgtggtct ttgcgcggga agcggtacac aatggtcccg
    14521 ggtgcgtcaa acatcctgcg ggtatagcgc aggtttgccc gcagacccaa gcccgccgtc
    14581 gaacgttttg actgcgccga cacgcaaacc cttgcggacg gaagcctgtt ccgcctcttt
    14641 cgtcatgttg tgcgaccagt ccgcaccgcc gtaaagcagc cagtctttcg gcgcggaata
    14701 catcagcgtc gcgcccgcca gcggcatatg gctgtcgtat cgggcggcgg tgcggtcttc
    14761 ctgataacgc ttccacatat tgcccgcgtt tagtgtcaac cgccagcgtt cgcccaagcg
    14821 ttgggagaaa tcggcattga agccgccgac gaaattgtat cggctgccgc ctaagaggtt
    14881 ttgcccgaca aacggcacga tgccgaacga gcgcgttacc gaacgatttt tatagccgag
    14941 cgacaggtgc aggcactgtt cgctgaaatc tttgttatcc caataatgca cgccgccgcc
    15001 gctgatgccg ccgtagagga aatgatgccc gcccgcattg atttcgcgcg acacgcctaa
    15061 gccctagcgc aaaccgtgtg ccttttgcgg caggctgtcg gcggttttcg tccagcttct
    15121 gcccgcaaat tcaatcgttt ttttggacga agcgttgttt atagcggatt aacaaaaatc
    15181 aggacaaggc gacgaagcct cagacagtac agatagtacg gaaccgattc act
//
LOCUS       DAWFIT010000046        15080 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFIT010000046 DAWFIT010000000
VERSION     DAWFIT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.046"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(664..948)
                     /locus_tag="WGQ85_001298"
     CDS             complement(664..948)
                     /locus_tag="WGQ85_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0750715.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            complement(1537..2802)
                     /gene="ftsY"
                     /locus_tag="WGQ85_001299"
     CDS             complement(1537..2802)
                     /gene="ftsY"
                     /locus_tag="WGQ85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0750716.1"
                     /translation="MFSFFRRKKKQETPAPEEAQIQETAAKAESETAQVIENIKEDVN
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            2948..4516
                     /gene="msrAB"
                     /locus_tag="WGQ85_001300"
     CDS             2948..4516
                     /gene="msrAB"
                     /locus_tag="WGQ85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0750717.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(4618..5103)
                     /locus_tag="WGQ85_001301"
     CDS             complement(4618..5103)
                     /locus_tag="WGQ85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0750718.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(5129..5977)
                     /locus_tag="WGQ85_001302"
     CDS             complement(5129..5977)
                     /locus_tag="WGQ85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0750719.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(6071..7072)
                     /locus_tag="WGQ85_001303"
     CDS             complement(6071..7072)
                     /locus_tag="WGQ85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750720.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            7131..7844
                     /locus_tag="WGQ85_001304"
     CDS             7131..7844
                     /locus_tag="WGQ85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0750721.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            7837..8109
                     /locus_tag="WGQ85_001305"
     CDS             7837..8109
                     /locus_tag="WGQ85_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0750722.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            8164..9534
                     /gene="glmU"
                     /locus_tag="WGQ85_001306"
     CDS             8164..9534
                     /gene="glmU"
                     /locus_tag="WGQ85_001306"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0750723.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            9610..9938
                     /locus_tag="WGQ85_001307"
                     /pseudo
     CDS             9610..9938
                     /locus_tag="WGQ85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
     gene            complement(10010..11275)
                     /gene="efeB"
                     /locus_tag="WGQ85_001308"
     CDS             complement(10010..11275)
                     /gene="efeB"
                     /locus_tag="WGQ85_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0750724.1"
                     /translation="MSTNNPTQPTRRTLFKTALAAGVGGAIGGYLGGKGLGETAERTA
                     ESQHSPQAYPCYGKNQAGIVTPQQAFSIMCAFDVTAQNAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(11419..12585)
                     /locus_tag="WGQ85_001309"
     CDS             complement(11419..12585)
                     /locus_tag="WGQ85_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0750725.1"
                     /translation="MRKFNLTTLSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            complement(12582..13421)
                     /locus_tag="WGQ85_001310"
     CDS             complement(12582..13421)
                     /locus_tag="WGQ85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0750726.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            13634..14959
                     /locus_tag="WGQ85_001311"
     CDS             13634..14959
                     /locus_tag="WGQ85_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0750727.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDQHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3540 a         4454 c         3773 g         3313 t
ORIGIN      
        1 aagtgggaat ccagaatctc taaagcttca gctaaccttt gaatattact gttgttctaa
       61 ggtctagatt cccgcctgcg cgggaatgac gggtctttta taacctttga atattgctgt
      121 tatcccaagg tctggattcc cgcctgcgcg ggaatgacga atccatccgc acggaaacct
      181 gcaccgcgtc attcccgcga acctacattc cgtcattccc acgaaagtgg gaatctagaa
      241 tcccggactt tcagataatc tttgaatatt gctgttattc taaggtctgg attcccgcct
      301 gcgcgggaat gacggtagag cggtttctgt tttttccaat aaattcctaa aactcaaaat
      361 ttcatcattc ctacaaaaac agaaaaccaa aaacagaaac ctaaaatccc gtcattccca
      421 cgaaagtggg aatccaggac gcggaatctc aagaaaccgt ttacccgata agtttccgtg
      481 ccgacagacc tagattcccg cctgcgcggg aatgacgggt cttttataat ctttgaatat
      541 tactgttgtt ctaaggtcta gattcccgcc tgcgcgggaa tgacggttta gaagttgccc
      601 gaaacctcaa aaaaaaaacc gaaaccgaac aagccggatt cccgcctgcg cgggaatgac
      661 gggctaaata atatcaaacc ataaatcctg ccaagaaaca ttattttctt caatcagttg
      721 caatttccaa gccctgttcc atttcttcaa ctgtttttcc cgagtaattg cactctccat
      781 cgtaggatgc agttcatacc aaaccagcat agtaacgttg taccgtgatg taaatccctc
      841 aatcaaatgc tccctatgtt ggtaaatacg ttgcaccaaa tcagatgtaa cgccaatgta
      901 taacgtgcca ttacgttggc ttgctaaaat ataaaccgca ggctgcatat aatacccttt
      961 tgaattattt caatttatat tcccgcgaac accatcccgt gattacttta acccttcgtt
     1021 attcccatag ctttccatca ttcccgcaac tcttcgtcat tcccgcgaaa gtgggaatct
     1081 agaacgcaaa atctaaagaa accgttttac ccgataagtt tccgcaccga caaacctaga
     1141 ttcccgcctg cgcgggaatg acggcggagc ggtttctgtt ttttccgata aattcctaaa
     1201 actcaaaatt tcatcattcc tacaaaaaca gaaaaacaaa atcagaaacc taaaatcccg
     1261 tcattcccac gaaagtggga atctaggacg cggaatctca agaaaccgtt tacccgataa
     1321 gtttccgtgc cgatggttct agattcccgc ctgcgcggga atgacaggtc ttttataaac
     1381 tttgaatatt gctgttatcc caaggtctgg attcccgcct acgcgggaat gacgggtctt
     1441 ttataaactt tgaatattgc cgttatccca aggtctggat ttccgcctgc gcgggaatga
     1501 cggcatcggt ctgctgtttt cggacggcat ttcggctcaa tccagcagtg cgtccacaaa
     1561 cgcgcgcgcg tcaaacgggc gcaaatcgtc tatgccttcg cccacgccga tatagcggac
     1621 ggggacgggg cggtcggaag caagcgcggc gaggatgccg ccttttgccg tgccgtcgag
     1681 tttggtaacg ataagccccg tcagccctaa tgcgtcgtca aaggctttga cttggttgac
     1741 ggcgttttgc ccgatgttgg catcaagcac gacgatgatt tcgtgcggcg cgtcgggcat
     1801 ggctttttgc agcacgcgtt tcactttttt gatttcttcc atcaagtgaa gctgcgtggg
     1861 caggcggccg gcggtgtcgg caagcacgat gtcgatgccg cgcgctttgg cggcttggac
     1921 ggcatcgaag cacacggctg cggaatcgcc cgtggtttgt gaaatcacgg ttacgttgtt
     1981 gcgttcgccc caagcttgaa gctgctcacg ggcggcggcg cggaaggtgt cgccggcggc
     2041 aagcagcaca gatttgccct gcgcttggaa atatttggcg agtttgccga tagacgtggt
     2101 tttgcccgcg ccgttgatgc cggcaagcat gatgacaaac ggctctttgg tttcgggcaa
     2161 gaccaatggt ttttccagag gcttaatcag gtcatacaag gcttctttca acgcgccgcg
     2221 caattcgttg ccgtctttca gccctttgag gctgacgcgg tcgcgcacgt ctttcatcag
     2281 gtattcggtg gcttccatgc ccatgtcgct ggtaatcagc acggtttcca gctcttcgta
     2341 caaatcttcg tcgatttgtc cgccgccgaa cacgcccgcc agcgatttcg ccattttgtc
     2401 gcgcgatttg gtcaaacctt gtttcaaacg cgccgcccaa ccgagcttgt gttcttcagt
     2461 tgtcgcaacg gcttcttgaa cttgcccgac agcctcgccg acggtttcgg caacggcttc
     2521 ttttgccgct tcgacttgtt ccgctgcttt ttccgccgct tcctctgctt cagacagcat
     2581 ctcggcaacg gtttccttta cctgttcaac cgcaccgctg acggtttcaa cggcagattc
     2641 gacctgccct ttgacgcttt ccgccaaaga attaacatct tctttaatat tttcaattac
     2701 ttgagcagtt tcggattctg cttttgctgc ggtttcctga atttgagcct cctctggagc
     2761 cggcgtttcc tgttttttct tgcgacggaa gaagctgaac attggatttt ccttttaatt
     2821 ttagaaactt gaaatagggc gtattgtagc gtattttacg cggcagggtt gtctgaaaat
     2881 ccgggctgta aggtttcggc atctcaaacg tctaatcatg caaaccgtcc acacaggaaa
     2941 catcaaaatg aaacaccgta ctttcttttc cctttgcgcc aagttcggct gcctgcttgc
     3001 gctgggtgct tgttcgccca aaatcgtcga tgccggagcc gcgaccgtgc cgcacacttt
     3061 atccactttg aaaaccgcgg acaaccgccc cgccagtgtt tacttgaaaa aagacaaacc
     3121 gacgctgatt aaattttggg cgagctggtg tcctttgtgt ctgtccgaat tgggacagac
     3181 cgaaaaatgg gcgcaagatg caaaattcag ctccgccaac ctgattaccg tcgcctcccc
     3241 gggctttttg cacgagaaaa aagacggcga ctttcaaaaa tggtatgccg gtttgaatta
     3301 tcccaagctg cccgtcgtga ccgacaacgg cggcacgatt gcccaaagcc tgaatatcag
     3361 cgtttacccc tcatgggcgt taatcggtaa agacggcgac gtgcagcgca tcgtcaaagg
     3421 cagcatcaac gaagcgcagg cgttggcgtt aatccgcgac ccgaatgccg atttgggcag
     3481 tttgaaacat tcgttctaca aacccgacac tcagaaaaag gattcagcaa tcatgaatac
     3541 acgcaccatc tacctcgccg gcggctgctt ctggggcttg gaagcctatt tccaacgcat
     3601 cgacggcgtg gttgacgcgg tatccggcta cgccaacggc aacacgaaaa atccgagcta
     3661 tgaagacgtg tcctaccgcc atacgggcca cgccgaaacc gtcaaagtga cctacgatgc
     3721 cgacaaactc agtctagacg acatcctgca atatttcttc cgtgtcgttg atccgaccag
     3781 cctcaacaaa cagggcaacg acaccggtac gcaataccgc agcggcgtgt actacaccga
     3841 ccccgccgaa aaagccgtca tcgccgccgc cctcaaacgc gagcagcaaa aataccaact
     3901 gcccctcgtt gttgaaaacg agccgctgaa aaacttctac gatgccgagg aataccatca
     3961 ggactacctg attaaaaacc ccaacggcta ctgccacatc gatatccgca aagccgacga
     4021 accgctgccg ggcaaaacca agaccgcccc gcaaggcaaa ggcttcgatg cggcaacgta
     4081 taaaaaaccg agtgacgccg aactcaaacg caccctgacc gaagagcaat accaagttac
     4141 ccaaaacagc gcgaccgaat atgccttcag ccacgaatac gaccatttgt tcaaacccgg
     4201 catttatgtg gacgttgtca gcggcgaacc tttgttcagc tccgccgaca aatatgattc
     4261 cggctgcggc tggccgagct tcacgcgccc gattgatgca aaatccgtta ccgaacacga
     4321 tgatttcagc tacaacatgc gccgcaccga agtgcgcagc cacgccgccg attcgcactt
     4381 ggggcacgtc ttccccgacg gcccgcgcga caaaggcgga ctgcgctact gcatcaacgg
     4441 cgcgagcttg aaattcatcc cgctggaaca aatggacgcg gcaggctacg gcgcgttgaa
     4501 gagtaaagtg aaataagccg caccgccgcc taacccgaca aaataccgtc tgaaacctgc
     4561 aacgtttcag acggtatttt ttatccggcg gggattctgt ccagacggag attttgttta
     4621 gacggcatcg ccgccgtttt caatcagccc cgccaaccgt tccaatgcga aggcgaccgc
     4681 ctgcgcacgg acggattcgc ggttgccgtc aaaacggcgc attgcttcgc aacttccgcc
     4741 tggaaaggca aacccgaacc aaaccgtgcc gacgggtttg ctttcgctgc cgccgcccgg
     4801 gccggcgatg ccggaaatac cgacggcgta atccgcctgc gccacggctt tcgcgccgcg
     4861 cgccatctca tagacggttt ggcggctgac cgcgccgtgt tcgagcaggg tttcgggcaa
     4921 cacgcccaag cggtcttctt tggctttgtt gctgtatgtt acaaaactct ggtcgaacca
     4981 ttgcgaactg cctgcaacgc ttgtgaatgc ggcggcaagc attccgcccg tacaggactc
     5041 ggcacagctt acggtttgac gttttttcgt caggtttcgg gcgatggtgt gcagcgcgtc
     5101 catttcccac tcccctttca gacggcgttt aagaattgat gatgtgtatg tcgcgttgcg
     5161 ggaacgggat gttgatattg actttgcgga ggttttcgac cacttgttcg ttcaagtcgc
     5221 attgcagcgt ccagcggtct gcttcgtttg cccaagccca taatgtgatt tcgatggcat
     5281 tgtcgcccaa ggcggtgatg taggcggcgg cctgccgctc ttcgttttga acgctcaagg
     5341 ggtgttcgac ggcggctttc aacaccgcct ctttcgccac tttcaaatcg cagttgtaat
     5401 cgacgccgac tatcacttgg gcgcggcaca gcggcagtgt ggaacggttg acgatgctgt
     5461 tgcccatcac cacgctgttg ggcagcacga cttcttcgtt gtcggtcgtc cgcaaagaag
     5521 tctgcaccat tttaatctct cggacatatc cttcaaaacc gccgacgcgg ataaaatcgc
     5581 cgactttgaa cgggcggaac aggataatca gcgcgccggc ggcaaaattg gacagctggt
     5641 ctttcaggga caacgccacc gccaaacccg cgccgccgat taaggcggtt acggatgttg
     5701 tggaaacgcc caatctgccc aatgcggcaa taatcaccaa aatcaataag ccgatattgg
     5761 caacattaca caaaaaacta atcagcgtgg catcgacctg cgcgcgcgtc atcgccgccc
     5821 tcatcacagc gacaatgcgt ttcgccgccc attttccgac caaaaaaata agcagcgcgg
     5881 cggcaaggtt cagcccgaac gcccacgcct tttcagccag atgctcccaa ccggaaacac
     5941 tgatcaggtg taaaaaatca aattgtttga agtccattgt tttcctcttg atcgaacatc
     6001 cgccccgtgc ggcgtaatcg gcacaggtgt aaaaatgccg tatgaagccc tgcggggcgg
     6061 tatgtttgtt ttatttcaaa accgttctaa tccaacggga aacccatccg cgctggttcg
     6121 catcaatatc gcgctgcgcg ggggcggtgg tgtgcgtcgg ggcttgggcg aagcggaaca
     6181 cgtcgggcag gcgcgtgttt ttgacggcgg ggtaaaccca catttcggac ggaaccgcct
     6241 gctgcacttc ccgactttgc agccattgca ccagttttgc cgccagcttc ggctgtttcg
     6301 cgcccttcaa gactgccgcg ccttcgacct ggcggaatac gccgcctttt aaaaacaggt
     6361 tgcccgtcgg cggctcgctg tatttgcctt tggaaaaata cacttccgcc gccgggctgg
     6421 cggcataacc gaccaccagc ggatacgcgc cgccgttgtg cgaaaagtcg gtgtaatacg
     6481 cctcgctcca gcctttggcg accttcacgc cgttctgccg catctgtgcc caccatttga
     6541 acgcgccttc ttcgcccaga ccgctgatgt tcgccatcag gaagcccagc cccggggacg
     6601 acgtggcggg agacggcacg accaataggt ttttatattc ggggcgggtc aaatcctgca
     6661 gggtttgcgg cagaggaagc tttttgcctt caaaccattt tttgtcgtaa ttgatggaca
     6721 catagccgta atcgaccgcc aaagccgaag gcagcccgac cgcgacgggg gcggatccgg
     6781 gttgcgccgc cgccaaaatg cccatttccc gcgccttgcc gatattggcg ttgtccaagc
     6841 cgtacaccgc gtcggcgatc gggttggcgc ggctcaaaat cagtttgttg agcatttcgt
     6901 tcgcgccgcc cgcctgaata atcgacacct tcgcatcgtt tgcccgctcg aagcgcgcaa
     6961 tcagcccttt gggcaggctg aacgacttat gcaccgccag cctgacttcc gtctgcgcct
     7021 gcaggtatgc cgaaaccgcc agcagcggca gcagccaaat tttccgtttc attccgagtc
     7081 ctcctctatt cgctgtaaaa taacattcta acaaattttc acggttcaaa atgcaagaaa
     7141 acccgaccgt gtggctgttc gacctcgaca acacgctgca caatgccgat gccggcatct
     7201 tcacactcat caaccgcgcc atgacacgct atatggcacg ccgcctcaaa ctctccgaat
     7261 ctgccgcgtc cgacctgcgt caagactatt ggcaccgcta cggcgcaacg ctcgccggac
     7321 tgcaaatcca ccatcccgaa atcgacatcg ccgaattttt gcgcgaaagc catccgatcg
     7381 atgcaatcct gaccaggctg cacggcatgg cggatacgga aaacacccta tgccgtctga
     7441 acgggcgcaa ggcggttttt tccaacggcc cgtcgtttta cgtccgtgcc gttgtcggcg
     7501 cactcggttt ggaaaaccgt ttcgacgcac tgttcggcac ggatgatttc gggctgctgt
     7561 acaaacccaa tccgcaagcg tatctcaatg tctgccgcct tttggacgta ccgcccgaat
     7621 gctgcattat ggtggacgac agcgcggaca acctgcatca ggcaaaagca ttgggcatga
     7681 aaaccgtccg gttcggtgca aaagtgcacg acctgccctt tatcgatgct tccgtaagcg
     7741 atatggcgca actggctcgg tatgcagaaa ctttgtcaga acaccgccaa aatcattaca
     7801 atactcgcac aacccccgaa aatacgaaag aatgttatgc gtaaaacctt ccttatcctg
     7861 atgactgccg ccgccctttt gtcgggctgc gcgtgggaaa cttatcaaga cggcaacggc
     7921 aagaccgccg tccgtcaaaa atatcccgcc ggcacgcccg tttattacca agacggcagc
     7981 tactcgaaaa atatgaacta caaccaatac cgtcccgaac gccatgccgt gttacccaac
     8041 caaaccggca acaacgccga cgaagagcat cgccaacact ggcaaaagcc caaatttcaa
     8101 aaccgataaa cccaccctat gccgtctgaa gccctttcag acggcattgc acaggaaaac
     8161 gctatgccgc aaaacacttt aaacatcgtc atcctcgccg ccggcaaagg cacgcgcatg
     8221 tattccaaaa tgcccaaagt gctgcactgc atcggcggca agcccatggt tgagcgcgtt
     8281 atcgacaccg ccgccgcact gaatccgcaa aacatctgcg tcgtcatcgg acacggcaaa
     8341 gagcaagttt tgaatactgt caaacgcgat gtcgtttggg ttgaacaaac cgaacagctc
     8401 ggcaccggcc acgccgtcaa aaccgccctg ccccaccttg ccgccgaagg ccgcacgctg
     8461 gtgctgtacg gcgacgttcc tttaattgac gttgaaaccc tcgaaaccct gctcgaagcc
     8521 gcaggcaacg aagtcgggct gttgaccgac gtgcccgccg acccgacagg cttggggcgc
     8581 atcatccgcg acggcaacgg cagcgtaacc gccatcgtcg aagaaaaaga cgccgatgcc
     8641 gcccaaaaag ccgtgaaaga aatcaacaca ggcatcctcg tcctgcccaa cgccaaactg
     8701 gaaaactggc tgaacagcct ctccagcaac aacgcacaag gcgaatacta tctgaccgac
     8761 ctcatcgcca aagccgttgc cgatggtatt aaagtccatc ccgtccaagt gcgcgcctcc
     8821 tacctcgccg ccggcgtgaa caacaaacgc cagctcgccg aactcgaacg catcttccaa
     8881 accgaacagg cgcaagaatt gctcaaagca ggcgtaaccc tgcgcgatcc ggcacgtttc
     8941 gatttacgag gccgtctgaa acacggacag gatgtcgtga ttgatgcaaa ctgtatcttt
     9001 gaaggcgaaa tcgagctcgg cgacaacgtc gaaatcggcg caaactgcgt catcaaaaac
     9061 gccaaaatcg gtgcaaacag caaaatcgcc cccttctccc acctcgaaga ctgcgaagtc
     9121 ggcgaaaaca accgaatcgg cccatacgcc cgtttgcgtc cgcaagccaa actagctaac
     9181 gacgtacacg tcggcaactt cgtcgaaatc aaaaacgccg ccatcggcaa aggcaccaaa
     9241 gccaaccacc tcacctacat cggcgatgcc gaagtcggca gcaaaaccaa cttcggcgcc
     9301 ggtacgatta ttgccaacta cgacggcgtg cacaaacaca aaaccgttat cggcgacgaa
     9361 gtccgcatcg gttcaaactg cgtcctagtc gcccccgtta ccctcggcaa caaagtaacc
     9421 acaggcgcag gcagcacgat tacccgcaat atcgaagaca acaaactcgc cctcgcccgc
     9481 gcccgccaaa ccgtcatcga aggatgggtg aggccggaaa aagacaaaca ataaagccat
     9541 gccgtctgaa gccggtttca ggtttcagac ggcatcccaa aaacaaacat ccgataagga
     9601 cggaaaacca tgtcattacc cccatgcccg caatgcgcct ccgaatacac ctatgaagac
     9661 ggcggacaat acatctgccc cgaatgcgcc cacgaatgga atgaaaccga atccgccgcc
     9721 gaccttgcgg ctcaagtgcg cgatgccaac ggcgcggcac tgcaaaacgg cgataccgtc
     9781 atcctcatca aagacctcaa ggtaaaaggc agctcgatgg tgatcaaaca aggtacaaaa
     9841 gtcaaaggca tacgcctgca ggaaggcgat cacacatcgg ctgcaaaatc gacggcagcg
     9901 cgatgaattt aaaatccgaa ttcgtcaaaa aagcctgacc gcccaaaaac aagaaacgcc
     9961 gtccgaaccc gtttggcaag gttcggacgg cgttttttat atggcggatt tatacgccca
    10021 gcagcccttg ccccaaaaag ccgccttttt ccacgccggg caagacgaag aaatagccgc
    10081 cgccgaaggg gctgatgtat tcttccagcg gttcgccgtt gaggaggttt tgcacgaaga
    10141 tgaatccgtc ggcaaggttt gcctgatagc agacgaacac cagtccgaca tcaagctgtc
    10201 cgcttgaggc gagtccgcgc gaatagctgt aggcgcggcg gaagaggcgg tgttttttga
    10261 ggaattcggg atcgcgcgga ttcgccaggc gtatatggct gtctttgggc gtggtattcc
    10321 cctcggggtc tttggcaaaa tccggttggt cggcttcttt tttgccgtcc atcggcgcac
    10381 cgctgtattt gcgccgcccg aaaatgtcgg tttgctcttg aagcggcgtc ctgtcccaaa
    10441 actcgacaaa gtggcggata aggcggactg cctgatagct gccgtttttc gcccactccg
    10501 gttcgtcgag gctgttggcg gccacacccg tccacaaaac ctcgtcggcg gttttggggt
    10561 cggaaacttt ggggttgccc gtgccgtccc tgaagcccaa caggttgcgc gccgccatcg
    10621 cgccgggttc ggatttgggc tgccacccgt cgatactcca acggataacg gcggtttgga
    10681 cggtgtgttt gatgatgtcg cgcagggcgg cttggcaggt ttcgggggta aaggcacaga
    10741 tttgtaggct caaatcgccg tcgcaccagc ttttttgcag cttatcgttg gagaagtcgc
    10801 gcatttcctg caaatgaatc ggttttttgt ctttgagtcc gaatcgtccg tcaaacaggc
    10861 tgctgcccac ccccacggta acggtcaatc cgtcggggtt gaaggctttg cccaaaatgc
    10921 cgctgccggc cggcggaagt ttgtcgtcgc cgtcttggta ttcaccgcct tgagtgagaa
    10981 actcgatacg ggcagtcagc gtgcggaaca ggttttccag ctgcttggcg ttttgcgcgg
    11041 taacgtcaaa ggcgcacatg atggaaaatg cctgctgcgg cgtaacgata cctgcctgat
    11101 tctttccgta acagggatag gcttggggcg agtgttggct ttcggcggtg cgttcggcag
    11161 tttcacccag ccccttccca ccgagataac ctccgattgc gccgcccacg ccggctgcga
    11221 gtgcggtttt aaagagggtg cgcctggtcg gttgtgtcgg gttgttcgta ctcatgtttt
    11281 tatcctttat ttaaactcgg tatccgatac ggcggaacgc gggaaacgga aacctccgcc
    11341 tttttcaata cctttccggt tttgcagata aaatttcaga cggcatctgt cgctaaatgc
    11401 cgtctgaacg cttaaggctt atttcaagcc gagtatgccg cgaagttggg caaggtcttc
    11461 ggcaagcgcg ttaatagggg cctgtaacgc tttgcggtcg gcttcgctca gcttgtcgta
    11521 ggtttcaaaa ccgtctttgg tgcggtattt cgccagaatt tcgttgacct gtttgaagtt
    11581 ggtatcggtt ttttccaaca aggctttgtt tttggcctca atcagcggac ggaacaaatc
    11641 gacgattttt ttcgaaccgt ccacattggc ttggaagtcg ctcaaatcgg tgtggctgta
    11701 ccggtcttct tcgccgctga ttttactgcc cgccacttct tcaatcagtt cggacgcgcc
    11761 gccgaccacc ttgcccggag gaaacgccaa tgcgtcgatt tctttttgca gggcttcgac
    11821 atcggtcatc agtttcgctg caatttcctt cacgccggac acgtcttttt ccacccaaag
    11881 ggcgtattcg atacggtgaa agccggtaaa tccggcatct ttcgcgccgt ctttgaagtc
    11941 gtcttcacgc gcatcgatga cggggtcgag ttcgctgaaa agctcggcaa tcggttcgat
    12001 gcgttcgtaa tggacgcggg tgtcggcaaa cagggatttc gccttttcaa tgtcgcctgc
    12061 tttgacggct tcggtaaagg ttttggtttt cgccaccagc tctttaacct cgccttgaac
    12121 gtaggcttta tagtcggcga gcggttggga cagtttttcc aaatccgctt cgttggcggt
    12181 gtctttaaag ccgctgtcgg ttaccaccag cttgccgcgc ggattggtca aaagaccgca
    12241 agtcatttcg tattcgcccg gcaacagggt gacggtcatt ttatcggaaa gtccgggggc
    12301 gatgttttcg cgctcgtcca ccaccatcac gcctttcagg atttcccatt cgagcttgcg
    12361 gccgctgttg tttttaatat tgaacacaac ctgtccgctc ggcacggtca gttccatcgg
    12421 ttcgcaggca ttgtcgttga cggcgatact gaccgaaccg ccctcgttgg cggtttgcgc
    12481 ctcaccggac gcggccggcg cagctttctc cgcctccggc ggctggcacg cggtcaaacc
    12541 taaggcaagc atcacggaca atgtggtcaa attgaatttt ctcatttcag ctcctcttta
    12601 cgggttaaag tttcagacgg cctgctgccg cacaaaaacc aagttatgac gggaataagg
    12661 tacagcagcc aaaccaaggc ctcgccctgc gtcggatggt cggtatagcc gaaaaatccg
    12721 ccgagcagca cacctaacgg actgtcttcg tgcaaatatt ttgatgagtc gaacacaatg
    12781 tcctgaagcg cgttccaaat gcctgcttcg tgcagcgcgc gcagcgagcc ggcaagcagg
    12841 ccggcggcaa cgacaatcaa aaacgccccc gtccaacgga aaaacttcgc cagattcagg
    12901 cgcatcccac cctgataaat caacgcgcca atcacggcgg cagccaaaac ccccgctacc
    12961 gcaccggccg gcatctgcca cgtcgggctc tgtttgaata cggcaagcag gaaaaaaacg
    13021 ctctccaaac cttcgcgcgc cacggcaaga aacgccatac cgaccaaggc ccatccttga
    13081 ccgctgccac ggttcaaagc cgcctgtaca gaatcctgaa gctgccgctt catcgaacgc
    13141 gccgcctttt tcatccataa aatcatataa gtcagcatcg cgacagcaac caaaccgata
    13201 atgccgacga cgaactcctg ctgcttctgg ggaatctcgc ccgttgccga atggatgccg
    13261 tatccgatgc ccaaacacat caaagaagca aggacgaccc cgaaccagac cttaggcatc
    13321 agtttggaat gtccggactg tttcagaaaa ccggcaacga tgccgacgat gagcgcggct
    13381 tcgataccct cgcgcaacat aattaaaaaa gcgaccagca taaacgcgaa cgaacaagga
    13441 tgatgaataa tatattatcg gaatattttc attgcttgta aatacaaatg caagttattt
    13501 ttatctgcag taccgcgcgg cggaaagttc cgcaggctgc agctgcgccc tgtgttaaaa
    13561 tcccctctcc acggctgccg caacgccgcc cgaaaccatc ttttttttat tactgccggc
    13621 aacattgtcc attatgaaaa aatacctgtt ccgcgccgcc ctgtgcggca tcgccgccgc
    13681 catcctcgcc gcctgccaaa gcaagagcat ccaaaccttt ccgcaacccg acacatccgt
    13741 catcaacggc ccggaccggc cggtcggcat ccccgacccc gccggaacga cggtcggcgg
    13801 cggcggggcc gtttataccg ttgtgccgca cctgtccctg ccccactggg cggcgcagga
    13861 tttcgccaaa agcctgcaat ccttccgcct cggctgcgcc aatttgaaaa accgccaagg
    13921 ctggcaggat gtgtgcgccc aagcctttca aacccccgtc cattcctttc aggcaaaaca
    13981 gttttttgaa cgctatttca cgccgtggca ggttgcaggc aacggaagcc ttgccggtac
    14041 ggttaccggc tattacgagc cggtgctgaa gggcgacgac aggcggacgg cacaagcccg
    14101 cttcccgatt tacggtattc ccgacgattt tatctccgtc cccctgcctg ccggtttgcg
    14161 gagcggaaaa gcccttgtcc gcatcaggca gacgggaaaa aacagcggca caatcgacaa
    14221 taccggcggc acacataccg ccgacctctc ccgattcccc atcaccgcac gcacaacggc
    14281 aatcaagggc aggtttgaag gaagccgctt cctcccttac cacacgcgaa accaaatcaa
    14341 cggcggcgcg cttgacggca aagcaccgat actcggttac gccgaagacc ccgtcgaact
    14401 ctttttcatg cacatccaag gctcgggccg tctgaaaacc ccgtccggca aatacatccg
    14461 catcggctat gccgacaaaa acgagcatcc ctatgtttcc atcggaaaat acatggcgga
    14521 taagggctac ctcaaactcg gacaaacctc catgcagggc attaagtctt atatgcggca
    14581 aaatccgcaa cgcctcgccg aagttttggg tcaaaacccc agctatatct ttttccgcga
    14641 gcttaccgga agcggcaatg acggccctgt cggcgcactg ggcacgccgc tgatggggga
    14701 atatgccggc gcagtcgacc agcactacat taccttgggt gcgcccttat ttgtcgccac
    14761 cgcccatccg gttacccgca aagccctcaa ccgcctgatt atggcgcagg ataccggcag
    14821 cgcgattaaa ggcgcggtgc gcgtggatta tttttgggga tacggcgacg aagccggcga
    14881 acttgccggc aaacagaaaa ccacgggtta cgtctggcag ctcctaccca acggtatgaa
    14941 gcccgaatac cgcccgtaaa ccggaaaaca atgccatctg aagcccatcc ctaggcaatc
    15001 gggcgcatta aggctaagtt ttgcgatacc gcaggcacaa aattcctttc ttatttgacg
    15061 gaattatagt ggatcaaatt
//
LOCUS       DAWFIT010000047        14911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFIT010000047 DAWFIT010000000
VERSION     DAWFIT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.047"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(94..3579)
                     /gene="smc"
                     /locus_tag="WGQ85_001312"
     CDS             complement(94..3579)
                     /gene="smc"
                     /locus_tag="WGQ85_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0750728.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEVSAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVRALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTRQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEERKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLTYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGITSELENLSPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIKRELAQLAEE
                     QTVLQHTSDGLADDIAILQEAAAELEYQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVQTLDEEYIAVQEKLAQIQAQGREQYAKVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3828..4550
                     /gene="lpxH"
                     /locus_tag="WGQ85_001313"
     CDS             3828..4550
                     /gene="lpxH"
                     /locus_tag="WGQ85_001313"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0750729.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4820..6406
                     /locus_tag="WGQ85_001314"
     CDS             4820..6406
                     /locus_tag="WGQ85_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0750730.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6575..6778)
                     /locus_tag="WGQ85_001315"
     CDS             complement(6575..6778)
                     /locus_tag="WGQ85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0750731.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6894..7391
                     /locus_tag="WGQ85_001316"
     CDS             6894..7391
                     /locus_tag="WGQ85_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0750732.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNWETFA
                     HIGQA"
     gene            7473..8666
                     /locus_tag="WGQ85_001317"
     CDS             7473..8666
                     /locus_tag="WGQ85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0750733.1"
                     /translation="MFFKHIEAALADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKHLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="WGQ85_001318"
     CDS             complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="WGQ85_001318"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0750734.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10124..10639
                     /gene="ybeY"
                     /locus_tag="WGQ85_001319"
     CDS             10124..10639
                     /gene="ybeY"
                     /locus_tag="WGQ85_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0750735.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10641..11465
                     /locus_tag="WGQ85_001320"
     CDS             10641..11465
                     /locus_tag="WGQ85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0750736.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11594..12973)
                     /gene="nhaC"
                     /locus_tag="WGQ85_001321"
     CDS             complement(11594..12973)
                     /gene="nhaC"
                     /locus_tag="WGQ85_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0750737.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13264..14487)
                     /locus_tag="WGQ85_001322"
     CDS             complement(13264..14487)
                     /locus_tag="WGQ85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0750738.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3509 a         4198 c         3845 g         3359 t
ORIGIN      
        1 gtaccgattt aaatttaatt cactataccg tctgaaaacc tgtatcgcaa cacgtcatta
       61 aaaacggcaa gattcagccg ttctgcaacc cgttcaaacg gcttccgcca tttccaacgc
      121 ttgtttgatg tccacggcga cgacgcgcga tacgcctttt tcctgcatcg ttacgccgac
      181 gagctgctct gccatttcca tcgtcaggcg gttgtgggag atgtagagga actgggtttg
      241 cgccgacatt tctttgacca ggttgcagaa gcgcgaggtg ttggcgtcgt ccagcggggc
      301 atcgacttcg tccaaaaggc agaacggagc ggggttgagg ctgaacagag cgaacacgag
      361 gctcatggcg gtgagggctt tttcgccgcc ggagaggagg tggatggtgc tgtttttctt
      421 gccgggcgga cgcgccatga tggatacgcc tgcggtcagg aggtcgtcgc ctatcatttt
      481 gagggtggct tcgccgccgc cgaacagggt ggggaagaag gtttggactt tgccgttgac
      541 ggcgtcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
      601 ttccaaaagg gtgatggctg cctgcacgtc ttcgctctgg ctgcggtagt agccgtcgcg
      661 ttcgcgcgct tcttcgagtt cttgcagggc ggcgaggttg acggcgccga gggcttcgat
      721 ttgccgggtg aggctgccta tgctgctgtt caatactttc ggcgactctt tcgccaacgc
      781 ttcgagcgcg tccaaatcgg cggcacgttc ggtcaggttt tgatggtagc gtttggcgtt
      841 aatcagggct tcctgctgct gcaacagggc ggtttgggtg gctgcctgaa gctgtggcaa
      901 ctgggtttgc agggtttgta ctttagcgta ttgttccctg ccctgcgcct gaatctgcgc
      961 gagtttttcc tgcacggcga tgtactcttc atccaaagtt tgtacggctt cggtcaactc
     1021 gtcgagtttt atgtgctgct cgtcgttttg gaactcggtt tcataggcga gggcaagctc
     1081 ttgctggcgt tcctgccaat caaaggtttg ctgttcaagt tgggcaattt gctgccggta
     1141 attttgtttt tgctggttga gcttgtggac ggcgacttcg gcaagcccgt attggcggtt
     1201 ggcttccagc agggcaagct gcgcctgttt cagacggcct tgctgctctt ggcggctgtg
     1261 cgcggcggtt tgctgttggt attcgagttc ggcggcggct tcctgcaaaa tggcaatgtc
     1321 gtctgcaagg ccgtcggacg tgtgttgcaa cacggtctgc tcttccgcca actgcgccag
     1381 ttcgcgcttg atgtgttcgc ggcggatttg cccttggttg gtacgcgcca gaagttcggc
     1441 ggcgcgttgc tgcgcctgac tgtattggcg cgtgtgctgc tgttgctgct gcatcaggtt
     1501 tttgtgctgc acttcggacg agcgcacggc ggcttccgcc tgtttgaacg cggcttcggc
     1561 ggcggaaagt tcgggggaga ggttttccag ttcggatgtg atgccgtcga ggcgcgcttt
     1621 ttgggcaatc aggctttcct gcgcgggttt ggcatagagc aggacgctga ctttgtcgac
     1681 ctgatggcct tcgggcgtga gccagatttg gtgtgcgccc aaatcgcttt gatgcgcgag
     1741 ggcgtaagtc aaatcgggcg cgcacaatac gccgtcgagc cagtggtgca atgccgtctg
     1801 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
     1861 gccgccagag agaccgtctg aaagccacgc cgcctgcccc tgcggcaaag gttcgggcgg
     1921 tacgaaacct tgcggcacgg cgcgggcgtg caggcgttct gcaaggatga cggacaaggc
     1981 gtgctgccac tcggcaggcg cggtgatgtg ttgccacagc tgcggcgcgg cggcgtggtc
     2041 ggttgcctgc cagaagtcgg cggcttcctg ctgttgcgac aggatttgcg acaacgcctg
     2101 ctgctgcgcc tgcaaggtga tgtgttgctg cttcaggctt tggaagcggc ttgaggccgt
     2161 ctgaaacgcc tcgcgggcgg cgtgtaaggc ttcttcggcg gcgatgattt gttcttcgta
     2221 atgctcttgc cggttttgca acagggcggc ggcttcctgc gcggcggcgg tttcggcttc
     2281 gtcgggcagg ttcagggctt ggttttcctg tttcagacgg cctttgcgct cttcgtgccc
     2341 ggcaatcgtt tgtccggtat gggcaagctg ctgctgcttc aacgccagtt cgcggcggat
     2401 gcggtttgcc tcgtcctgcc gggtttggaa ggcggcgttg agcgtggctt gggcttcttc
     2461 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccattccg ccaattcggt
     2521 ttgcttttct tcaacctgca actcgttttc ttcaagctgt acgcggattt gctgctgctc
     2581 ctgccggatg cgctgcaact gcgcctgtgc cgcctgcttg tcgcgttcga tgcgttggtg
     2641 caggttttgc cggtggcgga tttgttcttc caaacgggca atctgctcgc gcaacacgcc
     2701 gcgtttgttg cccagttcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaaggc
     2761 gcgtacttcg tcgtttaacg cctgaacctg agcggcggtt tcgtcctgct gcgcctgcaa
     2821 agattgatgc tgcgcggtcg ccttgtcggc ggcggcaagc gattgctgcc attgggcgta
     2881 atcgagcaaa tcctgttgct ggttcagctg cgcggtcagg gatttgtagc gttcggcggt
     2941 ttccgcctgt ttctccagct tttccacctg acgcgctaac tcgttctgca aatcgcccaa
     3001 acgctgcaaa tgctcgcgcg tgtctttcag acggccttcc gtctccttac ggcgttcctt
     3061 atatttggac acgcccgccg cttcctcgat ataggcgcgc aattcctccg gccgcgcttc
     3121 gatgatgcgc gaaatcatcc cctgctcgat aacggcataa ccgcgcgcgc ccacgcccgt
     3181 acccaaaaac aaatcggtaa tgtcgcggcg gcgcacggtc tgattgttga tgaaataagt
     3241 cgattcgccc tgacgcgtca gctgccgctt gatgctcacc tcggcatact gcccccacgc
     3301 cccctgcaaa ctgtggtcgc tgttgtcaaa taccagctcc accgaagccc tcggcgcagg
     3361 acggcgcgtc gccgcaccgt taaaaatcac gtcctgcata ctctcgccgc gaagctgctt
     3421 cgccgaaacc tcacccaaca cccagcgcac cgcgtcaatc acattcgact tgccgcaacc
     3481 gttcggcccg ataaccgcga caagctgccc cggaacatga atcgtggtcg ggtcggtaaa
     3541 agatttgaag ccggagagtt tgatgtgggt caggcgcata atggtcggaa aaaataaaaa
     3601 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
     3661 ccgcatcccg cacccgcgat gccgtctgaa gccttcagac ggcattttta acgcgcccga
     3721 accccggttc cgccaatgcc cgatgcttgg cgcggcattt gccgtattgc cccaccgccg
     3781 ggcgcgttat aatccgcccc gacatccgcc cgattggaaa cctgcctatg aaacccgcct
     3841 atttcatttc cgacctgcat ttgagcgaaa agcagcccga actgaccgcg ctgttgctgc
     3901 gttttttacg ttcttccgcc gccaggcagg cgcgggcggt ttacattttg ggcgatttgt
     3961 ttgatttttg ggtgggcgat gacgaagttt ccgagttgaa tacttcggtt gcgcgtgaaa
     4021 tcaggaaatt gtccgacaaa ggcgttgccg tgttcttcgt caggggcaac cgcgacttcc
     4081 tgatcggtca gaatttttgc cggcaggcgg gcatgacgct gctgccggat tactcggttt
     4141 tggacttgtt cggctgcaaa accctcatct gccacggcga cactctgtgt acagatgaca
     4201 gggcatacca acgtttccgc aaaatcgtgc atcggaagcg gctgcaaaaa ctgttcctaa
     4261 tgctgcccct gaagtggcgc acgcgccttg ccaccaaaat caggcgtgtc agcaaaatgg
     4321 aaaaacaggt caagcccgcc gatattatgg atgtcaatgc cgcctttacc gcgcggcagg
     4381 tacgcgcctt cggtgcggaa aggctgatac acggacacac ccaccgcgag catatccatc
     4441 acgaaaacgg ctttacccgc attgttttgg gcgactggca taacgactat gcttcaatcc
     4501 tccgcgtgga cggggacggc gcggtattcg tgccgctgga aaaatgctga aaatgccgtc
     4561 tgaagccttt cgggcggctt ttttttacac gcccctttcc cacactccct ccgccgcatc
     4621 atcaccatcc ttaacccgca ttaatgggcg ttatcgataa aatgcttgac cgattacata
     4681 agattacgta aagtgtgcaa acgcataaca attggtctta ccaatttatc ttttaaccaa
     4741 aatatcgggt gtacgggatt tgaacgtctt tccaaacctc gacgcgcctt atgatgcaaa
     4801 tgcctaaaaa ggattcgtta tggcactttt cctcagcata ttccccatcg tcctgctgat
     4861 ttggctgatg gtgaaaaaaa acagtatgcc ctcctacgtc gcgctgccga ttaccgcagt
     4921 gctgatttac gccatcaaac ttttctactt cgacgatgcg ggcatgctgc tcaacgccac
     4981 cgccgcttcc ggcctcgtca aaacgctcac gccgattacc gtgattttcg gcgcgattat
     5041 gttcaaccgt atgatggaaa ccacgggctg catcgatgtc atccgcaaat ggctggcgac
     5101 catcagcccg aaccccgtag cgcaactgat gattatcggc tgggcttttg cctttatgat
     5161 tgaaggcgca tccggcttcg gcacgcctgc cgcgattgcc gcgccgattc tgatgagctt
     5221 gggcttcaac ccgttgaaag tggcgatttt cactttggtg atgaactccg tccccgtctc
     5281 tttcggcgcg gtcggtacgc cgacctggtt cggtttcgca ccgctgaacc tgagtgccga
     5341 agacatcctc gccatcggca ggcagaccgg cgtaatgcac ttcttcgcag gtttcgtcat
     5401 ccccgtcatc ggcttgggct tcatcgtacc ttggtctgaa atccgcaaaa acttgggctt
     5461 cgtcgccatt gccgtcttct cctgcaccat tccttatgtc gcattggcga tggtcaacga
     5521 agaattcccg tcgctcgtcg ccggcgcgat cggcctgatg gtgtccgtat tcgccgccaa
     5581 ccaaggctgg ggcttgagca aagaccacgc caaagacccg aatgccgaaa aagtgccgtt
     5641 cgcccaagtc gccaaagcac tcgccccttt gggtatgctg atcggcatgc tggtggttac
     5701 gcgcatcaaa cagctcggca tcaaaggcat tttgaccagc aaagaagaat ggttcagctt
     5761 ccaactgccg tttgatttgt ccaaaatcac cgtcagcgac tccctgacga ttactttcgg
     5821 caatattttc ggacaagatg tcagcgcgtc ttaccaaacg ctgtacgtcc cggcttggat
     5881 tccgtttgtg ctgaccgttt ggatttgcat cctgctgtat aaaaccaaat tcaaagatgc
     5941 ctggacgatt tatgccgtaa ctttcaatca aaccaaaaaa ccgctgcttg ccctgatggg
     6001 cgcgctgatt atggttcagc tgatgctggt cggcggcgac aattcgatgg tgaaaatcat
     6061 cggtaaggaa tttgccgcaa tggcgggcga acactgggtt tatttctcgc cgtatctggg
     6121 cgcgattggc gcgttcttct ccggttccaa caccgtgtcc aacctgacct tcggcccgat
     6181 tcagcagcaa atcgccttgg ataccggcct gtccgttacc ctgattctgg cgttgcagtc
     6241 cgtcggcggc gcgatgggca atatggtgtg cctcaacaac atcatcgccg tatgtaccgt
     6301 attggatgtg aaaaattccg aaggtgcgat tatcaagaaa accgttatcc cgatggcgat
     6361 ttacggcgtg attgccgtcg tcgcggcaat gattttcttc ctctaggcga aacgccacca
     6421 tccatagcgg cagcacgcgg ggaacggaaa ccgccgttcc ccgtctgggc ggcgcgtcgg
     6481 gcagaatccc ggtgccggaa caagcccgcc ccgccaaaca aatgccgtct gaaaccggaa
     6541 aaggcttcag acggcatttt tccgccgctt gcgttcaggc gtaatgatga tgcggcatct
     6601 tgccgtcaag gcggtagcgc gtgccgcaat acgggcaggc aacgctgccc gattctcctt
     6661 cgcacaaagg caaaaacact ctcggatgcc cgttccactg ctcgttgtcg ggtcccgagc
     6721 aatacagcgg cagattttcc ggcaacacgg aaatttcctg cagattcaga ttgtccattt
     6781 gattttcctt tgcgtggttg gcgtgcgccc attttacgcc atcggcaggc taaaggatat
     6841 tttcggcgca aagccgcaat ccgctataat ccccactttt cagacggcat accatgactg
     6901 cgcttaccct tccggaagac atccgccaac aagaaccatc cgttctgctc tataccctcg
     6961 tttccgccta cctcgaacac accgcccaaa ccggcgacga atccctctcc tgcctgtccg
     7021 acgaccagca cacgctgacc gcattctgct acctcgacag ccaagtcgaa gaaggcggct
     7081 tcgtgcaact catcgcctcc ggttacggcg aatatatttt ccgcaacccg cttgccgaca
     7141 gcctgcgccg ctggaaaatc aaagccgtgc cgaaagtctt ggacaaagcc aaagccctct
     7201 acgaacaaca cggcgaaacc atcgaaacgc tcgccgacgg aggcgcagac atcgaatccc
     7261 tgcgcaaaca gttccccgac tttgaagaat gggacggcgc atactacgaa gccgccgaac
     7321 aagacctgcc cctgcttgca gaacacatcc tgtcaaactg ggaaaccttc gcccatatcg
     7381 ggcaggcgtg attgcgtctg tttccagccc gtgtaaaaca gcgtaaaatc ggcaaacccg
     7441 aatgaccttc cgtccccatc aaggagcaag ctatgttctt caagcacatc gaagccgccc
     7501 tcgccgatcc gattctcggt ttgggcgaag cattcaaagc cgaaacccgc cccgaaaaag
     7561 tcaacctcgg catcggcgtt tataaagacg catccggcgc gacacccatt gtcaaagccg
     7621 tcaaagaagc cgaaaaacac ctgttggaaa gtgaaaccac caaaaactac ctcaccatcg
     7681 acggcgttgc cgattacaac gcgcaaaccc aaatcctgct gttcggcaaa gaccacgaaa
     7741 tcatcgccag ccgccgcgcc aaaacagcgc aaagcctcgg cggtacgggc gcattgcgta
     7801 ttgcggccga gtttgccaaa cgccagttga acgcgcaaac catctggatt tccaatccga
     7861 cctggccaaa ccacaacgcc atcgccaagg cggtcggtat ccaagacaaa ccttatcgtt
     7921 attatgatgc cgccaaacac ggtttggatt gggacggcat gattgaggac ttgagccaag
     7981 cgcaaaaagg cgacatcgtc ctgctgcacg gctgctgcca caatcctacc ggcatcgacc
     8041 ctacgcccga acaatgggaa actttggcaa aactttctgc cgaaaaaggc tggttgccgc
     8101 tgtttgactt tgcctaccaa ggcttcggca atggtttgga agaagatgcc tacggcttgc
     8161 gcgtgttctt gaaacacaat acagaattgc tgattgccag ctcttattcc aaaaacttcg
     8221 gtatgtacaa cgagcgcgtc ggcgcgttca ctttggtggc cgaagatgaa gaaacagcag
     8281 cccgcgccca cagccaagtc aaaaccatca tccgtacctt gtattccaac ccggcttcac
     8341 acggtgcgaa caccattgcg ctggtgttga aaaatgatga tttgaaagca caatggattg
     8401 ccgaactcga tgaaatgcgc ggccgcatca aagccatgcg ccaaaaattt gtcgggttgc
     8461 tcaaagccaa aggtgcaagc caaaactttg atttcattat caaacaaaac ggtatgttct
     8521 ctttcagcgg cttgactccc gagcaagtcg accgcctgaa aaacgagttt gccatttatg
     8581 ccgtccgctc cggccgcatc aacgtcgccg gcattaccga cgacaacatc gattatctgt
     8641 gcgaaagcat cgtgaaagtc ttgtaaaaca aaagcgcata cgaaatgccg tctgaacctt
     8701 aaccagttca gacggcatct gttttttgtt aaaaaacaat aaaccataac aatttcaaac
     8761 aagctaaaaa acatctataa aatcaaccca atatgatgaa ctcaaataaa aaatacgaat
     8821 atcatcattc tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct
     8881 ttccgccgtc attcccgcgc aggcgggaat ccgggaaccc aatgctgcaa gaattgatta
     8941 gaaacaactg aaaccgagca aactggattc ccgcctgcgc gggaatgacg aaataaggcg
     9001 agtaaaactg atgaaacaaa ttgattaaaa acaataactt atattatttt atttaaattt
     9061 tcgataaaat caattttccg tattcaatac tgctccaatc aattcccgcg caccgtcgtc
     9121 cgccaatttc ttagcgacag cgcgtccgag cgcgtcggca tattcggcag gggcttgcgc
     9181 gtccgcctgc aacacaaccg aaccgtcggg gtgtccgacc agtccgcgca aggtcagcaa
     9241 gccgttttct tccgtgcaat atgcggccag cggcacttgg cagcttccgc ccaaagcgcg
     9301 cgccaaagct cgttcggccg taacgcaggc gtgtgtggta tcgtggttta gcggttttaa
     9361 aacctcatac aaatcttcgc ggtgcgcggc aatttcgata cccaatgcgc cttgtccggc
     9421 ggcaggcagg ctgtcggatt ccgacaaaat catgcggatg cgtccgtcca atttcagacg
     9481 ctgcaaaccg gcggcggcca aaataattgc gtcgtattcg ccgttatcga gtttggacaa
     9541 acgggtttgc acattgccgc gcaaaggttt gataagcaaa tgcggatagc gcgcgcgcaa
     9601 ctgggcttcg cggcgcaggc tggatgtacc gacgacagcg cctttgggca tttcttccaa
     9661 acgcgcgtat tggttggaca caaacgcgtc aaacggattg gcgcgttcgc cgatggcggc
     9721 aagcgcgaaa ccttcaggca aatccatcgg cacgtcctta atcgaatgca ccgccaaatc
     9781 ggcgcgcccg tcatataaag cctgttccaa ctctttgaca aacaagcctt taccgccgac
     9841 ttttgacaaa gttttgtcca aaatctgatc gccgcgcgtg gtcatgccca aaatctcgac
     9901 ttcgcaatcg ggatacagag ccttcagacg gccttggata tgctttgcct gccacatagc
     9961 aagcaggctt tcgcggctgg cgatgacgag ttttttcggg ttcatgttca atgcgttcaa
    10021 agaatggaaa cggcgtgaag tctatcatat tttgaacgtt tcttcagacg gcaccgtcag
    10081 gtctatgtta tatttacacc cacaaaacac gaaacccgcc acaatgaaac gcaccaaaaa
    10141 ataccctttc ttaacgttgc agcggcaacg tttccatttg aactttgaaa acgcctcttc
    10201 cgccgccggc atcccagccg aacgcgattt ctaccgctgg gcgtggtctg ccttgaaaaa
    10261 tgaataccgc cgcgccgaca tcagcctgat tcttctggac gaagaagaag cccgagccta
    10321 caaccgcgac taccgcggca aagattacgc caccaatgta ttgagtttcg cgctcaacga
    10381 aggcgaaatc ctgccctgcc aagtttcgga aaaactgtac ggcgatttga ttatctgccc
    10441 gcaagtggtt ttgaaagaag ccgccgaaca gggcaaaacg cccgaacggc attttgccca
    10501 cctgaccata cacggcgttt tgcacctgat gggctacgac cacatcgaag acgatgaagc
    10561 cgaaataatg gaagccgaag aaatccgcct gatgctggcg gcaggcttcc ccaaccccta
    10621 ccaagaggac gaatattaaa atggacggta cgcagtcgaa acccaagttt ttcgaacgcc
    10681 tgatctcccg acttgccggc gagcccgact ccgccgaaga cgtattgacc ctgctgcgcc
    10741 aagcgcacga gcaggaagtt tttgatgcgg atacgctttt aaggttggaa aaagtcctcg
    10801 atttttccga tttggaagtg cgcgatgcca tgattacgcg cagccgtatg aacgttttaa
    10861 aagaaaacga cagcatcgag cgcatcaccg cctacgtcat cgataccgcc cattcgcgct
    10921 tccccgtcat cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc
    10981 tcaaatatat gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt
    11041 tcgttcccga aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc
    11101 acatggcgat tgttatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca
    11161 tcatcgagca aatcgtcggc gaaatcgaag acgagtttga cgaagacgat agcgccgaca
    11221 atatccacgc cgtttcttcc gaacgctggc gcatccatgc agctaccgaa atcgaagaca
    11281 tcaacacctt cttcggcacg gaatacagca gcgaagaagc cgacaccatt ggcggcctgg
    11341 tcattcaaga gttgggacat ctgcccgtgc gcggcgaaaa agtccttatc ggcggtttgc
    11401 agttcaccgt cgcacgcgcc gacaaccgcc gcctgcatac gctgatggcg acccgcgtga
    11461 agtaagcacc gccgtttctg cacagtttag gatgacggta cgggcgtttt ctgtttcaat
    11521 ccgccccatc cgccaaacat aaaaatgccg tctgaaaccg ttgcaagttt cagacggcat
    11581 ttcgcttatc cgcttatttt ttgctcaaag tcagccccgt ccaaccgaat aacagggtca
    11641 aagccaaact caaatagcag aaaaaggcat acggcagata ttcccaaacc ggcacgccca
    11701 gcgcgtggct gatgaacacg ccgcatacgc tccacggtac gagcgggtta atcaccgtcc
    11761 ccgcatcttc cagcgtccgc gacagattgc gggaatgtag tccgagcctg tcgtaaacgg
    11821 gtttaaacgt ttctccagaa agcaggatgc tcaaatattg ttcgccaatc aggaaattaa
    11881 ccccgaccga agtcatggca acgctgaacg tcgcgcgtcc ggcattcgtc aagaaggtac
    11941 ggatggcctc caacagggaa ggaatcacac cgagcgcaaa caacagtccg cccaaactca
    12001 tcccgagaat cacgatggtt tgcgtgaaaa acatactttc caaaccgccg cgcgaaatca
    12061 gtttgacaac atctttaaac gcttcgcctt cgagtttgta gccgccgtaa aaccacgcac
    12121 cgagctgacg cagatcgggc gtgctgtgca gatacgttac agcaacggca accatgacgg
    12181 taaagagcat ggcgacgacg gcgttgatgc gcatcaatgc caaaatgacc aacagcgcaa
    12241 acggaatcag cgaatagccg tgcaccaatc ccgtggcttc aagctggctg cggaaggatt
    12301 cgacgctgtt caaatcctgc gcggcgacat tcggcaaaag ccaaagcatc agtgccgcac
    12361 taatgagcca cgcggggatg gtggtgtaca tcatattttt gatgtgctca aacaagtcga
    12421 tgccgacgat ggacgcggaa atacccgtcg tatccgaaag cggggacatt ttgtcgccaa
    12481 aaaatgcgcc cgaaacaatc gcgcccgccg tcatcgccat atcggcctga aacgccgccg
    12541 ccatccccat aaaggcaacg ccgacagtgg cgcaggtggt caggctgctg ccgatggaca
    12601 cgccgatgac ggaacacagc gcgaaggcgg aaaaataaaa ataagtcgga gaaatcagcc
    12661 cgaaaccgta atacatcagc gtcggaatcg caccgctcat catcagtgcg ctgaccatca
    12721 gcccgatgaa gaaaaacagg taaatcgcgc ccataccctg attcaacgcg cctatcatgc
    12781 cctgctgcat atcgttgtat ttcaaaccgc gcgccaagcc gtacaaaatc agcacgacga
    12841 tggcggcaat aatggacata tgcggcagcc actccaatga aatgatggta tagcccatcg
    12901 cggcaatcag agcgacgacg acggcaagtg cctcaccgcg cggcatatcg agtaaggatt
    12961 tgaaagcaaa catgattctt tgcccgattt gatataaagt taacggattc taacacacca
    13021 taccgaatcc gcccaacgtt ttcagacggc attttagcgt tttacaaaag ccggacagaa
    13081 acaaaacggc aattttctca atttaaatta atttttttga aatataattt tttatttttt
    13141 tgtttgttgg catataagac ttttatggat tgaagtatta aaacctcggc acacacgccg
    13201 caaacaccgg tacgcaaaaa tgccgtctga aagatgttca gacgacattt ctcaagaaaa
    13261 agattatttg tccgccctat atccgtacac cgcaaaaaac acgatataca gataacagat
    13321 gacggaaacg acaaacgaag acatcagggt gtaagtatct gccacccagc cctgcacgac
    13381 aggaacgacc gcaccgccga caatcgcggt acacagtaca ccggaagccg cgttggtaaa
    13441 ttttcccaat cctttggttg ccaaagagaa aatcgtcggg aacataatcg agttgaaaaa
    13501 accgatggca agcagcgacc acatcgccac atccgcattg ccgctacccg tcgccatcgc
    13561 gacggcaagc agtacgaccg cagccgatgc gttaaacgcc aaataacggt tgggcgcgaa
    13621 tttcgccatc accgccgaac cgaggaaacg tccgaccatc gcgccgcccc aatagaacga
    13681 caggtaatgc gcggcagaag catgatccag ccctttcaga taacccaata cgttgaccat
    13741 caacgaaccg atagacacct ccgcgccgac atagcagaaa atgcctgccg taccgaacac
    13801 gagatgtttg tattgccata cgctggtttt gccgtcgtgg ttgtgcgcgc tttcctcggc
    13861 ggcaattttg cgcgcgtcgg gcagccggat cattttcacg aaaacggcaa ggataatcag
    13921 cagccccgcc agtcccaaat agggaatctg tacggaagaa atctgttccg ccttgctgac
    13981 ggtttgggtt gcgtccgcca gaatcaggaa cgcgccgatt tgcggcgcaa tggtcgtacc
    14041 caaagcgtta aacgcctgaa ccagcgtcag tgttgccgat tccttgccgg gtttcgccag
    14101 cagggtaaca taaggattac cggcgacctg aagcagcgtt acgccggaag ccaaaataaa
    14161 caacgcgccc aaaaataccg cgtaagaatg gctacccgca gcaggataaa acagcaggca
    14221 tccgaccgcc gtcagcagaa agccgccgat aacgccgttt ttgtagccga ctttgccgac
    14281 aaaagccccc atcgggatgg acatcaccgc ataggcggta aagaaacaga attggatcag
    14341 catcgcctga acgtaagaca ggtcgaaaat ttctttcaaa tgagggataa ggatgtcgtt
    14401 catgcaggta ataaaaccca tcatgaagaa cagcgtggtc aagacgacca atggggacgt
    14461 atgattgttt tgtgattgcg cagacatgat ttgcctttcg tggtgtcgga atattacccg
    14521 tttcaagaaa atagaaattg acatttctta acggcaatgc aaatcatact gatatttgaa
    14581 cattttgcaa actttatcgg atgcaggcaa tgcgccgatc tgaaattcaa gcagcagaaa
    14641 ttaatatttt acagttgctt gcatagattt taatagattt taaaaaattc cggcggactt
    14701 gcaaaaaccg ttcccaataa aatgaaaatc cgccgccgta tccggaaaat tcggaaatgc
    14761 cgcatcgtgg catcaaaacc ggagcggaaa gaatgtcgta tgcccgacaa aaaaggggca
    14821 tacggctgtg ccgtattttt tgcaccgtcc tccgcccttc ggcgggggcg tgaaaatata
    14881 gtggattaac aaaaatcagg acaaggcgac g
//
LOCUS       DAWFIT010000048        14884 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFIT010000048 DAWFIT010000000
VERSION     DAWFIT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.048"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(148..843)
                     /locus_tag="WGQ85_001323"
     CDS             complement(148..843)
                     /locus_tag="WGQ85_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0750739.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1014..1673)
                     /locus_tag="WGQ85_001324"
     CDS             complement(1014..1673)
                     /locus_tag="WGQ85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0750740.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1871..3983)
                     /locus_tag="WGQ85_001325"
                     /pseudo
     CDS             complement(1871..3983)
                     /locus_tag="WGQ85_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4169..4774)
                     /locus_tag="WGQ85_001326"
     CDS             complement(4169..4774)
                     /locus_tag="WGQ85_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0750741.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4823..5221)
                     /locus_tag="WGQ85_001327"
     CDS             complement(4823..5221)
                     /locus_tag="WGQ85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0750742.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5329..6240
                     /locus_tag="WGQ85_001328"
     CDS             5329..6240
                     /locus_tag="WGQ85_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0750743.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6230..7531
                     /locus_tag="WGQ85_001329"
     CDS             6230..7531
                     /locus_tag="WGQ85_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0750744.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELNKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7594..7890
                     /locus_tag="WGQ85_001330"
     CDS             7594..7890
                     /locus_tag="WGQ85_001330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750745.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7924..10074
                     /gene="dinG"
                     /locus_tag="WGQ85_001331"
     CDS             7924..10074
                     /gene="dinG"
                     /locus_tag="WGQ85_001331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0750746.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10110..10724
                     /locus_tag="WGQ85_001332"
     CDS             10110..10724
                     /locus_tag="WGQ85_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0750747.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10819..12687)
                     /locus_tag="WGQ85_001333"
     CDS             complement(10819..12687)
                     /locus_tag="WGQ85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0750748.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12768..14138)
                     /gene="purB"
                     /locus_tag="WGQ85_001334"
     CDS             complement(12768..14138)
                     /gene="purB"
                     /locus_tag="WGQ85_001334"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0750749.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14210..14842)
                     /locus_tag="WGQ85_001335"
     CDS             complement(14210..14842)
                     /locus_tag="WGQ85_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0750750.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3547 a         4118 c         3835 g         3384 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccgct atatattctg atttaaaacc ataaggcttt
       61 aagcaatcat ctcttctata aagcctaaat acaaaaggcc gtctgaaatc ctattttcag
      121 atggccttta ctcattcaat cctcaactta ttgcggcttt ttctgctctt cgcgtacttt
      181 atccaccaac tggtcaatcg tggtcatacc ggactgccag tctttaaatt caacttggta
      241 tttgccgccg acaatcacag tcggtgtgcc gctgatttgg aatttattgg tcaactcttc
      301 catttgagcc gcacgcgctt ggctttcagg agcctcaaat gcagccaata cttttttgcc
      361 gtcaaacgct gtttgctcgg acagccattt tttcagggta tcggtatcgg ccagattgat
      421 tttttgatta accatcgcat cgaaaatatg gctgttggct ttatctgatt caccggccat
      481 ttccactgcg gccgccaaac gtgccaaagg tttcatttca tcaccccaca cgacatgctc
      541 ccggcgcata taggtatcgt ctttaaacgt tttgatgtgc tcgctcaaga ccggctcaag
      601 atgggcgcaa tgcgggcaga agtagccgaa aaattccaat acttcgattt taccggcctg
      661 ctgttgcgga ataggcgtag acaatacagt gtagttcaca ccttcgttca actcagcagg
      721 ggctgccgga gcagatgagc tgctttgggc gctgtctgcc ggaacactgg tttcagcctg
      781 tttgctacaa gcggccaatg ccaacagggt caatgaagtc aaagctaagg ttttcagttt
      841 cataggtatc tcttgtgtgt cagattaatg tgcggatttt atggcatttt attgaaggcg
      901 tgttcgattt gagtgaaaaa atgtgtttag ttttatttca gcctttataa aggccgtctg
      961 aaatatggct tagataatta gccttatttt cagacggcct ttgttctagt aatttacggt
     1021 ttcggacatt ttgtcttcaa tccgtcgatg tgttccatta agtcaggtgg gacatgattc
     1081 cacataggat caagaataaa atcgatacct tcccgtctgg ctaatttggc tgctgggaca
     1141 aaatcggaat caccagaaaa caagatgatt ttatcggctt gtttttttaa ggtaatagat
     1201 gaaatatcca agcctatacg catatctaca cctttttgtt taacatcgag cacgacatcg
     1261 ttttccgtaa tttgagttga cgggtttttc agaagatttt tcatgacttg gggttttaat
     1321 atccatgctg tattgtccga cagcttgcct aaacgtaacg ctaatttacg cttcttcttc
     1381 aattcggtat gcagattgca acgaaatatg gcttccggag tctttgaaaa atctatggca
     1441 cgatgagata cgggattgtg cattttcttt tcaaacggca cgcagtcgta aaagaaaatt
     1501 ctatataaat catccatttg atctgtgccg ttgattttat ctttcagatg cagcatcgaa
     1561 tatcggaaag ccaaatcagc aattcttttt gcatcgtgtg gattttgaga caggcctaat
     1621 gaccgtaccc gcttaataaa aaatgcaccg tcaatcaaaa tggcggtttt catattgctt
     1681 cccctatatt tgtcaaagat ataaaaaagc ccttgggatc ggcatacccg ctatgattta
     1741 atgggtaatg cgtactgcca agggcactat ttctttttgg attatactga tgtagataaa
     1801 gcctgtcaaa aagttcgtat gctgaattta cggattatca ggattttcag acggcctttg
     1861 cctgatttca tcagaaacgg taatttaccc gggcagtata gccgcgcgga ttacccggca
     1921 tagagtccga acgccaatat ttttgattga acagattggc tgcggcaaag gtaacgttaa
     1981 catttttatg gttccagcca agcatggcat caactcgggc aaagcctgga agcgtagtca
     2041 cttctttatt tcttgagtcg taaccgtagc gtttgcctgt accggttacg ccgatttcgc
     2101 cgtagaggtt ttcggtcggg gtataacgga aaaacaggtt gccggtaacg ttgctggtgt
     2161 tattcaaatg gatgcccact cggtcgggat tttctttgtc ttcaacgact ttcgcctgca
     2221 tcacgcccaa cgaaccgcgc agatagagtt tttttgggga tgatttgccc gatggcggac
     2281 aattccacgc cgcgcgaacg gtgtttgccg ctaaccgcat aaatataagg gttgtttttt
     2341 ggatcggggc ggtagcggat attgaagcgt tcgatttggt aggcagacaa cgtagtgctg
     2401 aggcggtcgt ccagccaact gcttttcacg ccggtttcgt attggcgggt gtactcgggg
     2461 tcggcgttga acacggcgga agacaacgta tcgatgctca aatagccgcc gcgtccgcca
     2521 taaggcgcga agcctttgtt atacgaggcg taaagtgtgt ggacgggatt gatgttccac
     2581 actgcgccga tgttggggct gaacgagtgt ccgctgtatt ggcggctgct gccggtgagt
     2641 ttgttttcgg aattaaaggt gtatttgtcg taacggccgc cgaggacgaa tttcaaatcg
     2701 ggcgtggcgg agaagatgtt ttgcacaaag atgccgtagg agtcggcttt gtggcggttt
     2761 tgggtcagaa taggctgcaa tctgcccgaa gccggccagc ttgcgcggtc gtaggggttg
     2821 atggaggcgg aaaaggcgct gctgaaaccc aatgtcgggt tgcggtgttc gcggctgtaa
     2881 tccatgccta cggtcaggtg gttttcaaaa cggccgatgg tgtagtcgcc gttgagcgtt
     2941 aagttggacg acagggtttt gttgtcggtc tgctgccagg cgtagttacg tttgattaag
     3001 ttgccatttt cgctgcctgc atagaaatga tcaaaatcct gcgccgccgt gcggtgggcg
     3061 agctgccatt gggcacgcca tttgtcgttg aaggcgtatt caaggtcgga acgccaaact
     3121 tgcagcttgt ctttgacaaa atcgttccgg tgggcgaacc ccatgcggta aggcagtccg
     3181 aagcggtcgt acacggactt ggtcggactg cggtcgggcg tgcgctccac attgtcgtag
     3241 gtgtattgcc ccgtccactt caagccgttg tcgagtttga cggtaatgct gggcgaaacc
     3301 atgacatttt tgctgtctat gccgctgcgg aacgaattgg cgcgcccgac ttcgccggtg
     3361 agacggatgg cgacgttttt gttcagcact tcgttgatgt ccatattcag gctgcggttt
     3421 gcccacgaac cataaaccgt accgatatta cggctttgtt tgaagttggc gtatttgctg
     3481 accatgttga tgacaccgcc gccgttggta cgcccataaa gcacggagga cggacctttc
     3541 aggatttcca cgcgctcgat gttggcggtg ctacggcgca cctgcccgct ttcgcgtacg
     3601 ccgtcgcggt aaatatcaga tgcgtcggct tgaaagccgc gcaggaaaat gctttcgccg
     3661 cgcatatcgt aggcggcgtc gatgccggca ttgccttcga ggatggaact caaatcgttc
     3721 gtgccgtaat ttttgttttt ctggatattg agcgtatcga tggtttgcgg cgtttctttg
     3781 atgagctgtc cgttgcgggt aacggcggct tcgtcgtagt tgatgtagcc tttgagtacg
     3841 ctggtgtcgg actgtccgac cacggaaacg gtaggtagcg tggcggtgta atgttcacca
     3901 ttgtcctgcg tatcggcggc agcaacaggg aaggaagcaa taatcagcgt gggtaataaa
     3961 gccaaatgaa atggtatttg catttttata ctcaatttaa caaaacaacc gaattatatt
     4021 gcctcacgga ggaaatgaga ataatttctt ttaactatat tgaacatgat atttgtaaac
     4081 aaaggtctca gaatgcggaa aactcgccgc ctgatactga aaaatgccgt ctgaacaggg
     4141 ttcagacggc atttttttga ccgcgaaatt atgcgccgaa cactttcaaa cgttctgcaa
     4201 ctggttcaaa ggtcttttca cctgccgccc cttcaatacc gccgataacc atttgtgcgc
     4261 gcaacaacca gttttcgggg atattccacg ctttggcaat cgccgcatcg ggcaagggat
     4321 tgtaatgttg caggtttgca cctacgccga ccgcggcaag tgtcgtccaa acggcatact
     4381 gcaccatcgc gttcgcctgg tccgcccaaa cgggaaagtt ggcggcataa gcagggaact
     4441 gctcctgcaa acctttgacg acattttgat cttcataaaa caaaatggtt gccgcacccg
     4501 ccttaaacag gttcaatttt tgcgcggtcg gttcaaaact gtcggcaggc acggcggcac
     4561 gcagcgcgtc ttcgacaaat tgccacacct tatcatgctc ttcgccaaac agcacgacca
     4621 cgcgggcaga ttgggaattg aacgaagaag gtgtgtgcaa aacggcgtgt tcgacgattt
     4681 ggacaacttc atctttgccg acgggcagat ttttatttaa cgaataaatg gaacggcggc
     4741 tttcggcagc ctgttgcaga gattgacggg tcatttcttt tcctcaaaaa cattcaacaa
     4801 aatacacttt aggcagtccg attcaggctt tacgcttgaa aaacagcctg tccagcgacc
     4861 atgctccgcc gcccgccgcc gcgatataga ggaatacgaa gcagaacagc actgcggact
     4921 cgccgccgtt ggcaatcggg aacaaagcat ttccggaagc gtgcgccata aaataggcaa
     4981 ccgccatctg gccggacaaa acaaacgcgg cagggcgcgc aaacaggccc aacaccagca
     5041 aaatgccgcc gacaatttct aaaataccgg caagcagcaa cagcccgccg ggcgaaccgc
     5101 tgcccatttc aatggggaag gcgaagattt tcgacgtacc gtgcaacaaa aacaggtagg
     5161 cggttacgat acgcaaaaca gaaagcaaaa ccggttggat acggttgcag caatcggaca
     5221 taaaagctcc tttgatattt gtcttcattt caggcggtag agtatagatg tttccccttt
     5281 tcaaatatat actgctttta ttgatacaac ttttccacac aagaaaatat ggacaccctg
     5341 ttcagcctca aggttttccg ccaagtcgtc caaagcggcg gcttcacccg cgccgccgac
     5401 gcgctcggca tctccaccgc aatggcaagc aaacacgtca gccacttgga aaacaccgtc
     5461 caagccaaac tcctgcaccg caacagccgc aacctcagcc tgaccggagc cggggaagaa
     5521 tactaccggc aatgcagtta cgcgctcgac acgctcgacg atgccgcgca aaaagccgcc
     5581 ggagggacgg aaaaaccgca ggggctgctg cgcgtaacga tgccgctgtg gtttgccggc
     5641 agccagatat gcaactggct ggcggaatac cgcgaacgtt atcccgaagt ggcattagaa
     5701 ctgattttgg acaaccgcca cgtcgatttg attgccgaag gcgtggattt ggcgttgcgc
     5761 gtttcccaaa ccctgtcccc gtcgctgatt gcgcgcccac tggcggaaat cgaatttgcc
     5821 ctgctcgcct cgcccgattt cctgaggcgc aacggcgtgc cggaaacgcc ggaagaagtg
     5881 gcggggctgc ccgccgtcct gccgacctac accaaccagc agaaactcga cctcacccgc
     5941 aaatcggacg gcaaaaaata ccggcttgaa ctgacccccg tcatccgtac cgacaacacg
     6001 ctgatgatgc gcgaaatgat taaggcgggc gcgtgcatcg gttatcagcc gctttgggcg
     6061 gcggaacacg atttgcgctg cggctcgctg gtgaggctgc tgcccgaata taccgtcccg
     6121 accgaccgcc tgaatgccgt ttatgcggac agggcgttct taagcgcgaa agtccgcagc
     6181 ttcatcgatt ttctgaacga aaaaatcgcc agcaggaaag gctgccgaaa tgccgtctga
     6241 aaccgcccgc cccctttatg cggacacgtg cgccgcacac acgctcgtct ggttccgcca
     6301 aaacctccgc atccgcgaca acgccgcctt atgcgccgcc gttgccgaag gtttgcccgt
     6361 tatcggtatt tggattgacg atgccgaaac agacaaccct cgccgcgccg cgttctaccg
     6421 ccaatccgcc gccgaactcg cccaagggct tgcagggcgc ggcatcccgc tctacacggc
     6481 ggcatcgcct gccgagctcg tccggctcgc cgtccgcctc aatatccgcg ccgtcatcgc
     6541 cgacgaatcc catacttttg ccgacaaact cgccgacaac gccctttggc atgaattgaa
     6601 caaacacggc atcgcgttaa ccttcgtcaa tgaccgcgcc gttttcggca aaaccgacct
     6661 gatacccgac ggcggcacgg cacacaccga tttcgaccgc taccgcgaag tatggctcga
     6721 ccgcttttcc aagcagcccc ccgccggtcc ggacctattc gcggcatacc gccaaccctt
     6781 ccccgaaaac ctttccgccc cgccgcctgc cgccctttca gacggcatct tcctgccgca
     6841 aaacagcggc gaaacggcgg cttggcggca gtggcggcgg tttctcgaac aggcggattc
     6901 ctactccgtt ttaaaggatt tcccctcgcg caaacacact tcgctgatgg gcgcgtattt
     6961 gagtgccggc tgcatctcgc cgcgcctgct cgcgcgggaa agcctcgaac gccgtctgaa
     7021 cgcgtgggcg gacaacatca tccgccgcga ttttttcctt caacttgcct tgcagcacac
     7081 ggatgacgac ccttcagacg gcaatcctga acacaccctg cgcctgacgc tttggcagca
     7141 gggccggacc ggcattccga ttatcgatgc cgcgatgcgc tgtttgcaca aaaccggcag
     7201 cctccacccc gccctgagac gcttgagcgc ggattttttc tgccacgttt taaacctccc
     7261 ccgccgcgaa ggcgagatat ggtttgcccg acagctgacc gatttcgatg cagcaatcaa
     7321 ccaaggcaac tggcggcttg ccgcctcacg gcacacctgc cccgacattg ccgccgccgc
     7381 acacaagacc gaccccgacg gcacctttgt cagacggcac attcccgagc ttgcccacct
     7441 gtccgccgac accgtccaca cgccttggcg gtttgcctgt tcggtcgata cccacggcta
     7501 tcccgcccat cctgtcgccg gtattcccta agcggtacgg caacctaaaa caaatgccgt
     7561 ctgaacttcc gtttcagacg gcattgcgtc cgaatgactc aatatcttcc gtttttttca
     7621 ctttcaatca tcaggtatgc ccaaatcacc gccacccaag ccgccaccgt tcccatcgtc
     7681 ggcaaaaaat gctgccaatc cgtcatcggc ggtacagggc aacggccgca ttcgatttcc
     7741 gcgccgccca acaaccggta cagcagcata tccgcaatac aaagcaacaa cgggtatttc
     7801 aaacgctgca accaaaaaac caaccacgtc cgcacgattt acctcccccc attccgacaa
     7861 cgatattgta ccgcttccgt gcaatgttaa aataacccca cttctaccgc cgtacaaaac
     7921 gccatgctca ccgatttaga aaaaaacgcc atccgcgacc attaccaaaa catcggcaaa
     7981 aacctgcccg gtttccgtcc gcgtgcttcg cagcgggaaa tgattgcggc ggttgccaac
     8041 gctttttcgc ggacgttgac gcgtgaagaa ggcgaagaag cacccaaacg tgagggcgag
     8101 agcattgccg tgatcgaagg cccgaccggc gtgggcaaat cgttggccta ccttttggcc
     8161 ggcggcatca tggcgcaaac gcgcggcaaa cggctgattg tcagcagcgc gacggtggct
     8221 ttgcaggagc agttggttga ccgagatttg ccgtttctgg tcgaaaaaag cggtttggag
     8281 ctgacctttg cgcttgccaa agggcgcggc cgttatcttt gtccgtacaa actctatcaa
     8341 ctgacgcaaa acaacgccca gcaaaacctg cttggttttg aagcgccgga agtgttgtgg
     8401 gacagcaaac ccaagcccga agaattgaac ctgctgcgcg acatcgccga cgaattttcc
     8461 gcccgacggt tcaacggcga ccgcgacacc tggccggaaa aaatcgatga cgcgatttgg
     8521 ctcaaagtta ccaacgaccg ccacggctgc ctgaaagccg cctgtcccaa ccgcgccgaa
     8581 tgcccgtttt acctggcacg cgatatgttg gaaaccgtcg atgtcgtcgt tgccaaccac
     8641 gatcttctgc ttgccgacat cagtatgggc ggcggcgtga ttctgcctgc gcccgaaaac
     8701 agtttctatt gcatagacga agcgcaccac ctgcccaaaa aagccctcag ccgttttgcc
     8761 gccgaacatt catggaatat tgccgtttgg acgctggaaa aactgccgca actgaccggc
     8821 aagattgccg cgctgaccga taaagccgaa cttgccaacc ttgccgacga agccgcctca
     8881 tccttactcg acagcctgca cgaatggcag ttccatttgg cggaagagcc gtctttgagc
     8941 ttggggccgt ctgaaaacga caggcgtacc aacagcgaac cgacttggct gtgggaagac
     9001 ggcaaaatcc ccgaaggcct cgaaaccacc gtttccaaca ccgccattgc cgcacgcagc
     9061 ctgctcaaac acgttatcgg gctgaacgat gcgctttccg ccgcgcgccg cgaaaaagaa
     9121 caagacagcg cgctcatcga ccgcctgacc ggcgagttcg gcctttttat ctcccgtatc
     9181 gaacaaatta gtgcggtttg ggatttgctc tccaccgttc ccattgaagg cgaagagccg
     9241 ttggcgaaat ggataacccg ccgtgccgac gataaaaacg actatatttt caacgccagc
     9301 cccatcagca gcgcgtccca ccttgccaac agcctgtggc ggcgggcggc aggcgcagtg
     9361 ttgacctccg ccaccctgca atccttgggc aactttaacc tgatgctgcg ccaaaccggg
     9421 ctgcaatggc tgcctgaaac caccaccctc gccctcaaaa gcccctttga ctttgaaaaa
     9481 cagggtgaac tctacatccc tcccatatac gccagcccca aagaccccga agcccacacc
     9541 gccgccatca tcgagtggct gcccaagctc atttcgcccg gcgaagccat cggcacgctc
     9601 gtcctgtttt cctcgcgcaa acaaatgcag gatgtcgcct tacaactgcc tgacgactat
     9661 ctgccgctct tgctcgtaca aggcgaattg cccaaagccg ttctcctgca aaaacaccac
     9721 cgggccatag aagaaggcaa agccagcatc atcttcggac tcgacagctt tgccgaagga
     9781 ctcgacctgc ccggcaccgc ctgcgtgcaa gtcatcatcg ccaaacttcc cttcgccatg
     9841 cccgacaacc ccatcgaaaa aacccaaaac cgctggatag aacagcgcgg cggcaaccct
     9901 ttcatcgaaa tcaccgtccc cgaagccggc atcaaactca tccaggccgt cggccgcctc
     9961 atccgcaccg aacaagacta cggccgcgta accatcctcg acaaccgcgt caaaacacag
    10021 cggtacggcc aacaattatt agccggcctg ccaccgttta aaaggatagg gtaaacctac
    10081 cgccctgccc gcaacagaaa ggaaaaatca tgaacttcac ccgactgctc aaccaagtct
    10141 taagcacggt tcaaaaaaaa ggcaacacct tctccgacag cccgctcaat tcattcggcg
    10201 gaggcgcgct ggttgccggt gtcgcctcca tgctgctgaa cggtaaaaac cgcaaaacca
    10261 tcaccaaaat cggttcgacc gccgctttgg gctacctcgc ctaccggggc tatcagatgt
    10321 ggcagcaaaa caaagggcgg gcaaccgtaa cacaaagcga tttccaacct gccggagaaa
    10381 ccgaagaaac atacagccgc accgtattgc gcaccatgat agccgccgcc gcttcagacg
    10441 gcatgataga cgaagccgaa cgccggacta tcgaacagga aagcggcaca gaccccgaaa
    10501 ctgctgcatg gctcgccgcc gaataccgcc tgcccgcaag catcgaagac atcgccgccg
    10561 ccgtcggcaa cgatgaggcg ttggcggcgg aggcctatct ggcggcaaga ctggtatgtg
    10621 ccgatttgtc gcggaaagaa accgtcttcc tcgcccgcct gtcgcaggct ttggaactgg
    10681 atgacaatct ggtggaaaac ttggaaaggc aattggggtt ttgattcaaa attccctccc
    10741 gcaggggaag gaaaataaaa accgccgccg ccccgaaagg caaacggcgg cacaaacgat
    10801 atcggacggc aaggcaggct actgcaatgc gccgtcgcga gaagatttaa gcaggcgttc
    10861 gtgtttgtga aattcccgcc ccatataata agaaccgaaa taggtcaggt ggtcttggtc
    10921 gccgtaaaga tagcggccgt atatttcgac cgtgtttttg ggcaggtatt tttgtgcgtc
    10981 cacccaatgc acattgggaa tatctttaat caaatcaaag accgcctgat tgctcttgcc
    11041 gatgtcgccc atagcctgaa tggggcggag atattggttt gcggcaaatc ttttcaattt
    11101 ttcctccctc aggggcgaac ggctgattga tgtgttgttt gcaaaaacat agacgggttt
    11161 gacggcggct atccttttga cggtttccct gaatcgggct gggaacccgg gtattaggaa
    11221 ggattgcgct tcaaatctcg gcacgggctg gccgcccatc ctcaaatcat agaattgggc
    11281 aatgaaaacg gcttcggctt tttcaacttc atcccggtat tttcgacata acgggttgtc
    11341 tgccagcttc tcatctaccc aaaccaaaca ctccgaatcg agggacagga ttttggcttt
    11401 ccacccttcc cggctgccga cataatccag aaacccccgc aggtgtccgg cgtgcgagtc
    11461 gccgagggtc aggacggttt ccggaaaatg attttccgca gcaaggggcg cgccgggcaa
    11521 cgggcggagg tgttcctgtt tcaatatccc ccttgcgtac aggttgtaac cgacaagtat
    11581 cagggacggg gcgagataga ggcagaaaaa tgcctttttg aaggtcatct tccgttttct
    11641 aagcggctgt tcaatcaaat aataactcaa cagggaaaat ccggccgtca acgcggcaac
    11701 cgccgatacg gcaggcagtc cgagctgttt gtcgcctgta atgtaatggg cgaaagcaat
    11761 aaaaatccaa tggtacaggt atagggaata agagattttg ccgacaaata cgatggggct
    11821 tgccgacagg atgcgggtcg gaagtgtccc gtattgcata ctccggataa gcagtgccgt
    11881 cagcaggcag ggaaggagca gggtcattcc cgggataaac ggattgtgtt tgtcaatcac
    11941 gaacaggcag gcaagcaatg cgccgaagca gagtgatgaa agcaactgcc gttttccatt
    12001 tgctgtttgc cgtctgccgt tttgcgtttg cccgtaaacc gccagcagcg aacctgccaa
    12061 cagctcggga aacctcagtg tcgaaaggta ataagtattg ggttggttga gaatatcggt
    12121 ataaaacccg cttggcaaaa acgatgtggc agtcaaaatc agaaatagga tgatgctgat
    12181 gttacgcagc acccgtagcg attttgtttt tttgcagcaa aatatcagca aaagaggata
    12241 caggaggtaa tactgttcct ctactgccaa agaccagata tgcagtacgg ggttctcgtc
    12301 ggcactcaaa tcgaaatacc cctgctgaaa ccccagataa atattggaca agaaaaccgc
    12361 agaaagctcc acggttttcc gcatttggtt gaaatcttcg taaaggaaga tttgagaggc
    12421 aatcaccgaa gccagcgaca cggccgcaat aaaagcagga taaatccgct taatcctgcg
    12481 ggtataaaaa tcccggaaag aaaaagaacc gttctgtatt tcagaaagaa tgatgccggt
    12541 aatgaggaat cctgagatga caaagaaaat gtccaccccc aggaatcctc cgggcagcca
    12601 gcggttattc aggtggaaaa tcatgacgga tagcacggcg acggcccgca atccgtcaat
    12661 ttccggtctg tatcggacag cttgcataaa tatcgcccct gtatgtcccg ttatcttaaa
    12721 aatgccgtct gaacgcgcgt tcagacggca tcggtttcag taaacgctca aatccgtttc
    12781 gccaacgctt cagccttgcc cacatacagc gcgggggtca gctcaagcaa tttggctttg
    12841 gcttcggcgg ggatttccag caatccgata aagcctttca gcacttcggg cgtgatgccg
    12901 cctttgccgc gcgtcaggtc tttcagcttc tcgtaaggat tggccacgcc gtaacggcgc
    12961 attacggttt gaatcggctc ggcgagcagc tcccaagtgg catccaaatc ggcggcaagc
    13021 gcggcggggt taggttcgag cttgttcaga ccgcgcaggt gggcggcgaa acccaacacg
    13081 gcatagccca cgcccacgcc catattgcgt aagacggtgc tgtcggtcag gtcgcgctgc
    13141 caacgggaaa tcggcagttt ttccgccaaa aaacccaata cggcgtttgc cataccaagg
    13201 ttgccctcgg agttttcaaa gtcgatgggg ttgactttgt gcggcatggt ggaagaacca
    13261 acttcgcccg ctttgacttt ttgtttgaag taccccaaag aaatgtagcc ccaaacgtcg
    13321 cggttaaagt cgatcaggat ggtgttgatg cggctgaggg tttggaagaa ttcagccatg
    13381 tagtcgtgcg gctcgatttg gatggtgtag gggttgaagg tcaggccaag gctgatttcg
    13441 acgaagttgc ggcagtgggt ttcccaatcg acgtcgggat aggcgaccat gtgggcgttg
    13501 tagttgccga ccgcgccgtt gattttgccg aggaactctt gcgcctgaag gcttttaaac
    13561 tggcgttgca ggcggtacac gacattggcg gtttctttgc ccaaagtggt cggcgtggcg
    13621 ggctggccgt gggtgcggct catcatcggg acggcggcaa ggtcgtgcgc catagcggtc
    13681 agtttttcga tgatttctgc cagtttgggc agcaaaacag cctcacgcgc ttcttgcagc
    13741 attaaagcgt gggacaggtt gttgatgtct tcgctggtgc aggcgaagtg gatgaactca
    13801 ctcacggcgg cgacttccgg cacttcggca aaacgttttt tcagccaata ttcaatggct
    13861 ttgacatcat gattggtcgt ggcttcgatg gctttgacgg cggccgcgtc ttccaatgaa
    13921 aagttttcaa tcaccgtgtc gatttcggca agcgtttcgg cactgaaggg cggcacttcg
    13981 gcaatcttcg gctcggcggc gagggctttg agccagttta attcgacttt gacgcgcgct
    14041 ttcatcaggc cgtactctga gaaaatcggg cgcaatgctt caacggattg ggcataacgg
    14101 ccgtctaaag gggaaagcga ggcgatgggg ttgatcattt cggcatcctg ttcgggaaag
    14161 acatcaaaaa ttgttaaatt gtttgcaatt atacatcact ctcaggacgc tatgccgtct
    14221 gaagcccgtg ttccgacaga tacgcaacga tttcgtcttc atcgtccgat atggcgaaca
    14281 aagaggctgc cccttcggaa atcagaccgc gcgccaaaag ctgcgcgttt atccactccg
    14341 ccaagcccga ccaaaacgcc tttccgacca aaacaatcgg acgcggcggc actttgcccg
    14401 tctgcaccaa ggtcaggatt tcaaacaatt cgtccagcgt cccgaagccg cccggcatca
    14461 cgacatatgc ttgggaatag cggaaaaaca ccgccttgcg ttcggcaaaa cgggaaaacc
    14521 gcaaggcgat gtcctgatac ggattcggtt tctgctcgtg cggcaaaacg atgttcagcc
    14581 ccaccgaaac cgacttcccg gcaaacgcgc ccttgtttgc cgcctccata atccccggcc
    14641 cgccgcccga aatgacggca atgcccgaat ccgacagccg ccgcgccaga cggcaggaga
    14701 acgcataatc cgcatgattc tgcggcgtgc gcgcgctgcc gaagatactg actgccggaa
    14761 acacgcccgc caatgcttcg tctgcctgcc tgcgttcggc atcataacgt gcctgctccg
    14821 gcacacggtt tgtattctcc attccatcct ccgttcaaaa acagcgattg tacaccgtca
    14881 aaaa
//
LOCUS       DAWFIT010000049        14822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFIT010000049 DAWFIT010000000
VERSION     DAWFIT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.049"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGQ85_001336"
     CDS             complement(210..1622)
                     /gene="trkA"
                     /locus_tag="WGQ85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0750751.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1720..3243)
                     /locus_tag="WGQ85_001337"
     CDS             complement(1720..3243)
                     /locus_tag="WGQ85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0750752.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            complement(3550..4356)
                     /locus_tag="WGQ85_001338"
     CDS             complement(3550..4356)
                     /locus_tag="WGQ85_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0750753.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4408..4659)
                     /locus_tag="WGQ85_001339"
     CDS             complement(4408..4659)
                     /locus_tag="WGQ85_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750754.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4759..5520
                     /locus_tag="WGQ85_001340"
     CDS             4759..5520
                     /locus_tag="WGQ85_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750755.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDIRKILNDIKYELKYKR"
     gene            5818..6987
                     /gene="metZ"
                     /locus_tag="WGQ85_001341"
     CDS             5818..6987
                     /gene="metZ"
                     /locus_tag="WGQ85_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0750756.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALESAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7073..7279
                     /locus_tag="WGQ85_001342"
     CDS             7073..7279
                     /locus_tag="WGQ85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0750757.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7378..7490)
                     /locus_tag="WGQ85_001343"
                     /pseudo
     CDS             complement(7378..7490)
                     /locus_tag="WGQ85_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8412..9164)
                     /locus_tag="WGQ85_001344"
     CDS             complement(8412..9164)
                     /locus_tag="WGQ85_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0750758.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9268..9735)
                     /locus_tag="WGQ85_001345"
     CDS             complement(9268..9735)
                     /locus_tag="WGQ85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0750759.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9787..11304)
                     /locus_tag="WGQ85_001346"
     CDS             complement(9787..11304)
                     /locus_tag="WGQ85_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ0750760.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGQ85_001347"
     CDS             complement(11325..13628)
                     /gene="parC"
                     /locus_tag="WGQ85_001347"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0750761.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     AIAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEAEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            13924..14607
                     /locus_tag="WGQ85_001348"
     CDS             13924..14607
                     /locus_tag="WGQ85_001348"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0750762.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
BASE COUNT         3117 a         3682 c         4382 g         3641 t
ORIGIN      
        1 tgaatattgc cgctgtccaa tggtttggat tcccgcctgt gcgggaatga cgatttggaa
       61 attacccgaa acccaaaaac aactgaaacc gaacagaccg gattcccgcc tgcgcgggaa
      121 taacggtggg ggacgatgcc gtctgaagcc cacctaccat tattccgctt ccataccgct
      181 tggcggcaat gtgtccggaa tggggcggtt tatccgaaaa agcccatttt gacttggatg
      241 agtttttcca gttcgttcag gatgcgccgg cgcgagacga aaaagatgat gtggtcgccg
      301 tcttggatga cggtttcggt atggtgtccc ataatggttt cgccggttcc ggcgcggacg
      361 acggcggcaa tgtggcagcc ttcgggccat ttgatgccgc tgatgcgcct gccgatgatg
      421 gcggaagttt ttttgtcgcc gtgtgcgacg acttcgatgg cttccgccgt gccgcgccgg
      481 atggggtgga cggcaacgat gtcgccgcgc cggatgtggg cgagtatcga gccgatggtg
      541 atgaggtggg gggagacgac gatgtcgatt ttgttgcctt cgagcaaatc gacgtagctt
      601 gagcggttga cgatgccgat gacgcgcttc gcgccgaggt ttttcgccaa aagggcggac
      661 ataatgttgc tttcgtcgtc gttggtcagg gcgcagaata cgtcgatttc gtcgatgtat
      721 tcgttgtcga gcagggtttc gtcggttgcc gaaccttgca ggacgagggt gttgtcgagg
      781 ttttcggcta tccattcggc acggcgcggc cggcattcga tgattttgac gttgtatgcg
      841 tgttcgagct gcttggcgag acggtagccg atgttgccgc cgccggcaat catgatgcgg
      901 cgggtgctgg tttctttggg gcgcaattcg ggtatgaccg cgccgatgtt ttcggcggcg
      961 gcggcaaata ggatttcgtc gccttcgatg atgacggttt gcggcgcggg gacgatgagg
     1021 cggttgttgc ggtaaacggc gcagatttgg cagtcggccc cgtcgggcaa atcttgggcg
     1081 atgtcggcaa tgctgcgtcc gacaagcagt ccgccgcgcc gcgcctgtat gatgaccatc
     1141 cgcacgcggt cgtctgcaaa acgtaaaacc tgcaatgcgc ccgggcagtc tatcaggccg
     1201 gcaagctgtt cggtaacgag ctgttcgggg ctgatggttt cggttatgcc gaatatggaa
     1261 aggctgccgt tttcgttgtt ttcgagcttg gggctgaggt attcgaggta ttcgctggaa
     1321 cggacgcgcg cgatgcggcc ggggatgttg aacaggtcgg cggcaacttt gcaggcgacg
     1381 atgttggttt cgtcgctgcg ggagagcgcg agcagcaagt cggcatcttc cgcgccggcg
     1441 cgttctaatg tgaaggggga tgcgccgttg ccgaaaacgg tttggacatc gaggcggctg
     1501 cctgtttcct gcaatgcttt ttcgtcgatg tcgataacgg ttacgtcgtt gttgggtatg
     1561 gcggcaaggt tttgtgcgac ggtagaacct acctgtccgt tgcctaaaat gaggattttc
     1621 acggtgtggg tcgccgagtg atgaaatggt ggattgtacc gcaaccaggg gcggaaatgc
     1681 cgtctgaaag cagattttgt gttcagacgg catgacgcct caagatttga cggggatgat
     1741 gccgattttc gcctgccatt ggggcggggc ggtggcgtgg atggattcgc ctttgctgtc
     1801 tacggcgacg gttacgggca tgtctttgac ttcaaattcg taaatggctt ccatgcccaa
     1861 ttcggggaac gccaagactt tggaagattt gatggctttt gccacgagat acgccgcgcc
     1921 gccgactgcc atgaggtaca cggctttgtt gtcggcgatg gcttcgcagg tggccacgcc
     1981 gcgctcggat ttgccgatca tgcccaagag gtcggtttgt tcgagcattt ggcgggtgaa
     2041 tttgtccatg cgggtggctg tggtcggacc tgccggaccg acgacttcat cgccgaccgg
     2101 atcgacgggg ccgacgtagt aaatcaggcg gttggtgaaa tcgacgggca attcttcgcc
     2161 tttgttgagc atatcgacga ggcgtttgtg tgcggcatcg cggccggtga ggattttgcc
     2221 gttcaacagc aatacgtcgc cggttttcca gcttgccact tcttctttgg tcagcttgtc
     2281 gacatcgacg cgtttgccgt tgtcggggct gtaagtcaaa tcgggccagt cttcgacgcg
     2341 cggcggcgtg agttcgacag ggcctgagcc gtccaattca aattcgacgt ggcgggtggc
     2401 ggcgcagttc ggaatcatgg caatcggttt ggaggcggcg tgggtcgggt aatcgaggat
     2461 tttcacgtcc aacacggtgg tcagaccgcc caagccttgc gcgccgatgc ctagcgcgtt
     2521 gactttttca aagagttcga ggcgcagggc ttcggtggtg gacaattccg cgccggacgc
     2581 ggctttttcc tgcaattctt ggatgtcgat gtggctcatc agggattctt tcgccatcaa
     2641 cacggctttt tcgggcgtac cgccgatgcc gatgcccaag atgccgggag gacaccagcc
     2701 cgcgcccatg gtcgggatgg ttttcaatac ccaatcgacg atgttgtcgg aaggattgag
     2761 catggcgagt ttggatttgt tttcagagcc gccgcctttt gccgcgcagg ttacttcgac
     2821 tttgtcgccc ggcacgatgc tcatatggat gacggcgggc gtgttgtctt tggtattttg
     2881 gcgtttgccg gcggggtcgg cgagaacgga agcgcgcagc gtattgcctt cccaagtgta
     2941 ggcgcggcgc acgccttcgt taaccatttc ttccacgctc atatccgcat cccactgcac
     3001 atccataccg actttcaaaa acacggttgc gataccggta tcttggcaga tggggcggtt
     3061 gttttccgca cacatacggc tgttgaccaa aatctgcgtc atcgcgtctt tggcggcggg
     3121 attttcttcc ttctgccacg ccttataaag cgcgtcgatg tagtctttcg gatggtagta
     3181 gctgatgaat tggaaggcat cgcagatact ttggataaag tcttcttgct tgatgacggt
     3241 catgattgtg ttccttttca aggttgtggc ggtaagtgtc tgctgctgtt ttaagcaacc
     3301 tttttgaagg ctgtttttta tcaggttgaa atagtgccag cgtttgtacg gtaaagacaa
     3361 tacataattc taatctcttg attatcaatt tgttttaata aaatactgtt ttactcggat
     3421 tttgatattt gtaatctttg cctgttgctt tgcggtaggg agaaatcagc aggcagttca
     3481 ttgtaggccg attgatgaac aatgccgtct gaaagggctt cagacggcat tgtgttcgga
     3541 cgggcgggct tattttgcgg cctgtccgtc ttcttttgca aaatatttgg cgtaaatctt
     3601 gtcgtattcg ccgctttcgc gtactttttc caacgcatcg ttcagcattt tgacggttgc
     3661 ttcgtcgcct ttgcgtaccg cgatgccgta gtgttcggtg gtgaagtcgg gcagggtaac
     3721 gaagtccatc cctttggtcg gattgttttt cacataattg gcgatgaccg cgctgtcgct
     3781 gactacggaa tccaagccgc cgttttccag ttctttgata atcaggggaa cgttttcaaa
     3841 gcgcgcgatt ttcgggttgt cgttgcccaa gagtttggat acggagaaat cgcccgtgta
     3901 gccggttacc acgccgactt tgttcatgtt tttcaaatcg tcggaagaag atattttttt
     3961 gcctttcgga acgaggacga cttgggtgat ttcaaaatac gggtcgctga agtccataga
     4021 ctgtttgcgg tcgtcggtaa tggttacgcc cgacatcaca acgtccgcat cgccgttgtt
     4081 caaggcgggg aaaaggctgt cccacggctg gtgtttgaat tcgattttaa aattgcccgc
     4141 cttcgccatc gcgttcatca aatccacatc gaaaccttcg acattgcctt tcgagtctaa
     4201 agattcaaag ggggcaaact cggcgttgga agccacgcgg tacactttgt cggggtttgc
     4261 ggcgggcgcg gcggcatctt tgccctgacc gccgcaggca gacagcgcga gtgcggaaca
     4321 ggcaagggcg gcggcaatcc attttttcat attcatcaaa attcctttcg gtaatcaaaa
     4381 agcctaaaaa atcagtttaa cacaaaatta acgttctttt tcacggaaaa acgcataagt
     4441 aacgtcgtgg aacaaatcgg tatgggggct gtcctcatag ctcaattctg ccaaggcgtc
     4501 ctcaaaagca agctgaatcg actcgaccgc gatttcggcg gtttcggcgg gaagggcgcg
     4561 gattaaggct ttgaatgcgg tggacaggac gtggttctgc atacgcagca cgtcgttcgc
     4621 ctcttccaga tattccaaac gctgttcgat gctgcccatg atgtatcctc gcgaatctgt
     4681 taaaatgcgc cattatatca aaccgcgctt tgggcgcggg tttgtctgcc tgcaaagatt
     4741 aagaaccgga agccgaaaat ggatgcgaat aaagagccga attgggaaaa acgtttggaa
     4801 aacctgcgtc agacgcgccg tatgaaggca gtgccgactt acgcgccgct gtcccgccct
     4861 tctgaaaccg gaacggacgc gtctgtgccg aaactggacg atgcaagccg aaaccgtgtg
     4921 ttaaaggcgt atctgaagaa atggcaggag gagcacaatg ccgccgttgc cgattgcggg
     4981 gaagaaatcg aaaccgaccc gatgatcgtc cttcaggaaa actggctcaa cgcgcaggct
     5041 tccctgcaaa tgtcggtttc ggaaaaacac atcgaatcgc ggcggagaat ccggtttgat
     5101 gccaaaaaag acagtgccga atttgaaacc ggacagattg aaggcgggga aaacgccgat
     5161 gcggcagaat cgggtacggc ggcagacggg gttgcggaaa acggcgaagg cggtgcggaa
     5221 gaagcgttga tgcccgtcca aatcaatatc ctgaacccga aagccgtcaa ccggcgcgaa
     5281 gtgttctgcc tgtccgaaca ggagctgacc gagcggctga tcaaacgcct gcgcccgcat
     5341 ctgaccgata ccgtcaacgg catcatccgt acggcggtgc aaaagcagat ggcggttttt
     5401 acctaccagc tccagcagat gctgcacgag caggcgggtg cggtagtcga agacgtgttg
     5461 gaacacgata tccgcaaaat cctcaacgac atcaagtacg agttaaaata caaacgctga
     5521 ttgccatgcc gtctgaaagc cgtttcagac ggcattttcc tgcccgtccg acttgaaagc
     5581 ctcatggaac aggcgcaaaa tattaatata gtggattaac aaaaatcagg acaaggcgac
     5641 gaagccgaag acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
     5701 gaatcgttct ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat
     5761 aaattaaaca acgggcgcac gctccgttgc cgaacggata aaaccgagga atcaccgatg
     5821 agcaaaaaac tccatccgca aacgctcgcc atacgcggcg gcaaagaaca aaccggatac
     5881 cgcgaacaca atcaggcatt gttcctgacc agcagtttca tgtgggacaa cgcccaacac
     5941 gctgccgatt tgttttcaaa aaaaatcaaa ggcttcactt atacccgtac cgccaacccg
     6001 accaccgccg cctttgaaaa acgcatcgcc gctttggaaa gtgcggaacg cgcggtcgcc
     6061 acttcgaccg gcatgtccgc gattcaggcg gcgtttttta cctttttgca ggcgggcgac
     6121 catgtgattt ccagccgcag cctgttcggc acgaccgtcg gctttatcaa taacatcgtt
     6181 accaaattcg gcatcgaagt gagccacgtg tcgccgactg atataaacga atggaaagcc
     6241 gccgtcaaag ccaacaccaa actgctgttt ttggaaacgc cgtccaatcc cttgggagaa
     6301 gtggccgact tggaagcctt ggcggaattg gcgcacggca tcggcgcgat tttggtggtg
     6361 gacaacagcc tgctgtcgcc catcggctct cagcctttga agcacggcgc agatatttcc
     6421 gtttcttccg caaccaaagc catagacgga cacggacgcg tgatgggcgg cgtgttggcg
     6481 ggttcggaag aactgatggc gcaggccgcc gtttactgca actcttgcgg gctggcgatg
     6541 tcgccgttta acgcgtggca gttgttgagc ggcgtggaaa ccctgtcgct gcgtatggaa
     6601 aaacagttcg acaacgccct gaaaatcgcg caatggctgc aagcgcagcc gcaggttcaa
     6661 gccgtgtatt acaccggatt gcccgaccat ccgcaggcgg cgcttatccg caaacagcaa
     6721 aacggcggtg gcatcgtcat cggctttgaa gtagccgacc aagaagccgc gtggaaagtt
     6781 gtggacggcg tggaactctt ttcccgaacc gccaacctcg gcgacgtgcg ctcgaccatc
     6841 acccatccgt ggacaaccac gcacggcagg atgcagcccg aagaaaaact cgccgccaac
     6901 atccgcccgg gactggtgcg cctgtcggtc ggtttggaat atgttggcga tttgattgac
     6961 gatttgaaac aggcactcgc acgctgattg cgtgtttcag acggcattgg acagcagatg
     7021 ccgtctgaaa cccttatcgc gtatggcata cacatcaaca ataggaaaaa atatggatga
     7081 agcagttttt gccgattggg cgttgaaaat ctgcctgacc ggcttgatta tttttttggg
     7141 ttttatcgtt tggaatttgg gcaaggagtc caaagcgggc aaattcggca tcgccatttt
     7201 gtttttggtt ttgggcttgg gcgtgttcgg ttttgtgttc aaagagttat tgattaagtt
     7261 tttggtattg ccgaaatagg tttgcggcct gtcaaaatgc cgtctgaaat cataaaaagt
     7321 aatctgcacc ctaaaagttt gatttaaccg ccaactgatt aaggtgcgga tttttatgac
     7381 aaaatatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt
     7441 actgtctgcg gcttcgtcgc cttgtcttga tttaaattta atccactata aaaatcatct
     7501 ttccgcagtc attcccacgg acgcgaaagt ctgtcttttt gaatcccgga taattttaca
     7561 tcgtcattcc cgcgcaggcg ggaatccaaa tcattgggta gcggcaatgt tcaaaagtcg
     7621 tctgaaaaat cagaagttct agattcccgt tttcacggga atgacggaat ttcagacggc
     7681 atcctcccgc cccgtcattc ccgcgcaggc gggaatctag tccgttcggt ttcggtcatt
     7741 tacgataaat tcctgttgct tttcatttct ggattcccac ttccgtggga atgacggagg
     7801 agcggataaa ttctcgcaat ctaaaacctt gtcatttcca taaaaaacag caacccgaaa
     7861 cagcaactta aaaacagcaa cctgaaacct cgtcattccc gcgcaggtgg gaatccagat
     7921 cattgggtgg cggcaatgtt caaaagtcgt ctgaaaaatc agaagttcta gattcccgtt
     7981 ttcacgggaa tgacggaatt tcagacggca tctgcccgcc ccgtcattcc cgcgtaggcg
     8041 ggaatctagt ccgttcggtt tcggtcattt acgataaatt cctgttgcct ttcatttcta
     8101 gattcccact tccgtgggaa tgacggcgga gcggataaat tctcgcaatc taaaacctcg
     8161 tcatttccat aaaaaacagc aacccgaaac agcaacctga aaccttgtca ttctcgcgca
     8221 ggcgggaatc tagtccgttc ggtttcggtc atttacgata aattcctgtt gcttttcatt
     8281 tctggattcc cacttccgtg ggaatgacaa actttaaggt gtgatgattt atccaaaaca
     8341 gtcgaaacgc aaaaaccggt ttctcgtttg cataagaacg gcaaattttt cggtgtcttg
     8401 ttttatgggc gttatccctt gtccgtcccg tagggcaggt aaacgtcgaa gcgggtgctt
     8461 ttgcctgtgt attggtaggc agggtcttgt ccggcaaggt gttcgccgag gcgggggcgt
     8521 ttgacgacga cgcgtttttt tgctgtttgg cgtgcggtat gcaggagtgc cgcttcatct
     8581 tgcgcttcgc cgacgagccg gtggaagtag gtcatttctt ttttaacggc ggcacttttg
     8641 cggcgttcgg gatacatggg gtcgagatag acgatgtcgg gtttgccttg tgtttggaca
     8701 agtgcgggca tttgttcggc ggcgttgccg aaatggaggt tgatgtgcgc ggcggtgttt
     8761 tgcgtttcgg gattgaggag ggcgcggcgg atgccgtctg aaagcaggca ggcgacggcg
     8821 ggatgttgct cgaaggcggt aacggccagc ccgagcgagg cgaggacgaa gctgtcgcgc
     8881 cccaatcctg cggttgcgtc ccaaacggtg gggtgcgcgg tgtggttgac ggctttggcg
     8941 atgagttcgc ccccgccttt tgtgcgccgg tattgtgccg cgccggaggc aaaatcgacg
     9001 atgacgctgc ttttttcccc gacaagcctg aggctgacgg tatcgtgttc ggcaaggagg
     9061 taactgcctt gttcgggcgg ttgggaaacg ggcacaaggg ggaatgcccg tatcagggtg
     9121 cggacggttt cggtggcggt gttgtcaata aggatgtcgg tcatggtgtt ttgtgtccgt
     9181 tcgggcgtgg ggcgaacccg ctttcccgtg ccgtttcaga cggcattttt tcgggtgggc
     9241 ggagatggtg cggatgccgt tttgtcgtca gccgatgttg aggcgttcca tacggtagcg
     9301 catggaacgg aagctgatgc ccaagcgttt ggcggcctgc gtgcggttgc cttcggtttg
     9361 tttgagggtt tgttcgatga tgtcgcgttc gatttggtcg agatagtcct gtatctgcat
     9421 ggtatcgggg tcgaacgcgc cgcaacgcaa cgcttcctgt gcctgatcgg cgttgccgaa
     9481 ggcggtgatg acggcaaccg gcgtatcgag caggcggctg ttgatgtgtt ggacgacttc
     9541 cagccccgag ccgtccggca tacgcatatc ggtcaggacg agcgaatagc gttggctgtc
     9601 gagcttgttt ttggcttcgg caacgcctga cgcggtatgg acgcgcaagc ccattttcat
     9661 cagggtcatt tccatcaggt cgagaatgtc ggtttcgtca tcgacgacga ggacggggtg
     9721 ttgcagttca gtcattggtt ttttccggta atgtgagttc gaaacatttg gcttccggta
     9781 ggtaggtcaa atcgccgaaa ttggcgtgcg ccagttcgcg ggcgacatac agcccgaggc
     9841 cggtgccgtt ttccgccgtg gtgtaaaagg gttcgaacag gtgttcctgc acttcgggcg
     9901 gtcggcaaag aggatacaga cggtgttttt ttgcgcgggg cggatggtga cggaaatcga
     9961 gccgggctgt ttgcggctgt gccgccacgc gttgttggcg aggttccaca taatttgccg
    10021 caggtgggcg ggatcgaaat aggcggtcgg gctgccgcct tgaatgtccg gacggatgca
    10081 gtcggcggca tcgggatggc cgagcaggaa ctcttgtttg aattcttccc aaaacggtat
    10141 caggccgatg gtttcgcgtt cggttttgtt gcgcttgttg agcgaggaaa tgtcttcgag
    10201 cattttgtcg atgcggcaga tgttgccgtc gatgattttg cacaatttgg cgttgaacgg
    10261 atctgccgcc cccgcttcca tattttcgcg cagcaggtcg ttggcgtggc ggatggcgga
    10321 catcgggttg cggatttcgt gggcgaggtt ggcggtcagt tgtccgagcg cggcaagttt
    10381 gacggacagg gcttctgcct gaatttcgct ttgcgggcgg atgtagagga tgagcagctt
    10441 gtccgaccct ttgttgaccg gaacggcgcg gatgccggcg ttcaggccgg gtgtgccgag
    10501 gacgtattcg aaagtgtgcg gagaggcttg atcccacagt tcggcggcag aatcggacag
    10561 accggcgcgc cgtccgattt cgagcgcggg gaaaaggtct ttcgccttcc ggttgaacag
    10621 caccgccagc ccttcggcat tgatgacgac gacagcttcc tgaacgcgtt cgagtacggt
    10681 ttggctcaag cctttgatgc ggtggtaggc gatatggctg tcgtaggcga gttttccggc
    10741 acggtcgatg tatttgaccg acagtgaggt gaagatggcg gaaagatagg aggcggcgac
    10801 aaggatgaag gttgccgagg cggttcgggc atccgatatg agcgggtatt tgccgatatc
    10861 gccgtcggca atggcgttga atatcagcag gatggcggcg tagctggaat agagcagggg
    10921 gtagcgcccg tagctgagca ggcaggagca gacgacgaag ggcaggatca ggatgccgaa
    10981 gccggaatcg atgccgccga acaggtaggt cagcacgccg atcatcgtga tgtccgccac
    11041 tgcgctgaaa cgcggcattt tcagcgactg ccattgccaa tgcggatgga taatggaaaa
    11101 gaaaatcagc cagcaggcaa tggcgagcca aacataaaac gcccacgccg tccacgaagc
    11161 gtgcaggttc gcacggctgc cggtttccag cccgaggatg tgcataatca tcagcgggaa
    11221 aacgatggcg acgcggatga tgttgatcag attgggaatc cggtctttga gtttttcaag
    11281 ttcgcggggg ttggaaatta ccataattat gtgcggaatc gggtttattt cggagaagaa
    11341 agctgtttca ggctgcccga tatgggcaat agtttgcctt ttttgccgcg ttttgcctca
    11401 agcagggaga tggggatgcg gtctttgtgc gctgcgccgc gcctgccttc gctttcaatc
    11461 aggatttctg cctcggaaga aacggcggta tgcgtcatcg attcgccggc gtttaatccg
    11521 atgatttgca gtcctttgcc ttttgccata attttcaatt cgccgatggg gaaggcgatg
    11581 gcgcggtttt gactggtggc ggcaatgatt ttgcaatcgg ggttgatgaa cgaggaggca
    11641 tagacggcaa cgggcggcag gacggtttcg ccactgtctg cggtcatgac cactttgccc
    11701 gctttcacgc gcccgaccat atcgcccagt ttggcaatga agccgtagcc gccgctgctt
    11761 gataataaat aatgttgttc cggcaatcct gtcaacatcg caacgggttt cgcgccgttt
    11821 tgcaactcga tcaaggatga aaccggcacg ccgtcgccgc gtccgccggg aatttcggcg
    11881 gcatcgagtg tataggttct gcccaatgaa tcgaggatga cgacgggtaa aaccgttctg
    11941 ccttcaaggg tttgtttgag gcggtcgcct tctttgaacg cggtttggct caaatcgaga
    12001 ttgtgtccgg cacggctgcg tatccagcct ttttccgaca gaatcagtgt gatgggttcg
    12061 tcggcggtgg tttgtgtcag cacggcgcgt ccggcctctt ccaccagcgt gcggcgtgca
    12121 tcgccgtatt gtttcatatc cgcctgcatc tctttgataa tcagcttgcg cttttcgttt
    12181 tcatcgccca aaaggatgtt cagacggcct tgttcttcgc gcaattcgtt caattctttt
    12241 tcgagtttga aaccttccaa acgtgccaac tggcgcaggc ggatttccaa aatgtcttcg
    12301 gcttggattt cggtcagccc gaacgccgcc atcaaatcgg ctttcgggtc atctgattcg
    12361 cggatgactt taatcacttc gtcgatgtgc agaaagactt tcagacggcc ttcgaggatg
    12421 tgcagccgtt tttccacttg gttcaaacgg aatttcagac ggcgtgttac ggttacggtg
    12481 cggaaatcca gccattcctg caaaatcgtt ttcaggtttt tctgcgcggg gcggttgtcc
    12541 agccccatca tcaccaagtt catggacaca ttgccttcca gcgaagtttg cgccatcagc
    12601 gtgttgatga aggtatcggt atcgatgcgg ctggatttcg gttcgaacac cagtcgcacg
    12661 ggatgttcgc cgtcggactc gtcgcgcacg cggtcgatta aatccagcat cagctttttg
    12721 gtgttgagct ggtcttggtt gagctgtttc ttacccgctt tcggtttcgg gttggtttgc
    12781 tcttcgattt cggcaaggat tttggcggaa ttggcgttcg gcggcagctc ggttacgatg
    12841 acgcgccact gtccgcgcgc caatttttcg atttcataac gcgcacgcac gcgcacgctg
    12901 cctttgccgg tttcgtaaat acggcgcaat tcgtccgccg gcgtgatgat ttgaccgccg
    12961 ccggcaaaat caggtgcggg aatgtattgc atcaggtcgg cggtttccag cgtcggcttt
    13021 ttcaacaacg caatcgccgc ctgcgtcact tcgttcaaat tgtgcgacgg aatctcggtc
    13081 gccatgccca ccgcaatgcc tgacgcgccg ttgagcaaca ccataggcag gcgggcgggc
    13141 aggtgcagcg gttcgtcaaa cgcgccgtcg tagttcggca caaaatccac cgtcccctga
    13201 ttgatttcgg acaacagcaa ttccgcaatc ggcgtcagcc gcgcttcggt gtaacgcatc
    13261 gccgccgccc cgtcgccgtc gcgcgagccg aagttgccga tgccgtcgat taagggatag
    13321 cgcaaggtaa aatcctgcgc catccgcacc atcgcctcat aggcggaact gtcgccgtgc
    13381 gggtggtatt tacccaaaat ctcgccgacc acgcgcgccg atttcaccgg cttcgccccc
    13441 gccgtcaaac ccatatcgcg catggcaaac aaaatgcgcc gctgaacggg cttttggccg
    13501 tctgaaactt caggcagcgc gcggcctttg accacgctca tggcgtattc gagataggcg
    13561 cgttcggcgt atcggccgag catcagcgta ttggaatcgg tatgtggagc gtgcggttgc
    13621 atatttatcg tatttccttt tcggttgaac ccccgccact tggacatctg tccttcgggg
    13681 cggtagaatc agactttatt tgggaggggg caaccccttc caaatcaggg caacacatag
    13741 ggcggtgctt tatgtgtcgt cctgtgtgtt gaaacattgt gtatgcggaa tgtaaagcaa
    13801 agggcattat tgtaaatcaa aacggcaaac catagtgggc gcgtgtctga atctgaatca
    13861 aatacgggaa tgtgaaaata tgttataaat aaggctttcc cactttcaca cattggagac
    13921 gatatggaat tggtatttat ccgccacgga caaagcgaat ggaacgcgaa aaacctgttt
    13981 acaggctggc gcgacgtgaa gctgagcgag caggggcttg ccgaggctgc tgccgccggt
    14041 aaaaaactga aagaaaacgg ctatgagttc gacatcgcct tcacatccgt cctgacccgc
    14101 gcgattaaga cctgcaacat cgttttggaa gaatccgacc aactgttcgt accgcaaatc
    14161 aaaacatggc ggctgaacga acgccactac ggccgactgc aaggcctgga caaaaaacaa
    14221 accgccgaac aatacggcga cgagcaagtc cgcatctggc ggcgcagcta cgacaccctg
    14281 ccgccgcttt tggacaaaga cgatgagttt tccgcacaca aagaccgccg ctatgcccat
    14341 ctgcctgccg atgttatccc cgacggcgaa aacctgcaag taacgctgga gcgcgtattg
    14401 ccgttttggg aagaccaaat cgcccccgcg attttgagcg gcaaacgcgt cttggtggcg
    14461 gcgcacggca actccctgcg cgcgctggca aaacacatcg agggcatttc cgacgaagac
    14521 atcatgggct tggaaatccc gaccggtcag ccgttggtgt acaaattgga cgacaatttg
    14581 aaagtcatcg aaaaattcta cctgtaaggg tgtgaaataa aaaatgccgt ctgaaggctt
    14641 gagcgttcag acggcatttt tgacggtaaa cgtagcaact gctttcgcga gaacgacggg
    14701 attttaggtt tttgtttttc tgttttcgtg ggaaggatgg gttttaggtt gcggtcattt
    14761 atcggaaaag cagaaaccgc tccgccgtca ttcccgcgca ggcgggaatc tagaacctct
    14821 ga
//
LOCUS       DAWFIT010000050        14721 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFIT010000050 DAWFIT010000000
VERSION     DAWFIT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.050"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            136..2016
                     /locus_tag="WGQ85_001349"
     CDS             136..2016
                     /locus_tag="WGQ85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0750763.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQVQAADIDTDLLWEVCGGEEFAAEAIAEEYYGHTPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAETLKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPGDKMEQVIMQRLSTVYFPNGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(3023..4048)
                     /locus_tag="WGQ85_001350"
     CDS             complement(3023..4048)
                     /locus_tag="WGQ85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0750764.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALK
                     PGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(4153..6561)
                     /locus_tag="WGQ85_001351"
     CDS             complement(4153..6561)
                     /locus_tag="WGQ85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0750765.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            6631..7635
                     /locus_tag="WGQ85_001352"
     CDS             6631..7635
                     /locus_tag="WGQ85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0750766.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(7692..8390)
                     /locus_tag="WGQ85_001353"
     CDS             complement(7692..8390)
                     /locus_tag="WGQ85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0750767.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            complement(8684..8791)
                     /locus_tag="WGQ85_001354"
                     /pseudo
     CDS             complement(8684..8791)
                     /locus_tag="WGQ85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8849..10387
                     /gene="murJ"
                     /locus_tag="WGQ85_001355"
     CDS             8849..10387
                     /gene="murJ"
                     /locus_tag="WGQ85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0750768.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            10519..11475
                     /locus_tag="WGQ85_001356"
     CDS             10519..11475
                     /locus_tag="WGQ85_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0750769.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            11526..12308
                     /gene="trpC"
                     /locus_tag="WGQ85_001357"
     CDS             11526..12308
                     /gene="trpC"
                     /locus_tag="WGQ85_001357"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0750770.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            12374..14674
                     /gene="recQ"
                     /locus_tag="WGQ85_001358"
     CDS             12374..14674
                     /gene="recQ"
                     /locus_tag="WGQ85_001358"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0750771.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLGMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSICETAACRRVLLLKHFGEASEPCG
                     RCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIQRNGHDKLS
                     TFGIGSEQTDKEWRSVIRQCISLGYLTVNVEQHQALQLTEAAKKVLKGETGVMLRPLK
                     RDKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHSVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         3098 a         4155 c         4131 g         3337 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctcccc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggtgc aggcggcgga catcgacacc gatttattgt gggaagtatg cggcggagag
      361 gaatttgccg ccgaagccat cgccgaagaa tattacggcc atacgccgac caaaaccgag
      421 ctggcggcga ctttgattgc gctttacgcc gcaccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacaac aggacgcgca aatcgacgct tgggcggaaa ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcttacacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctccgccta cgaactggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg ttttcccgac cttccgcttc ctgaaatgcc cgacctgccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttggg caacggcacg aagcgcgtcg gcatccacat tgccgcgccg
      961 tcgcttgccg ttaaaccggg cgacaaaatg gaacaagtca ttatgcagcg gttgagtacg
     1021 gtttattttc ccaacggcaa aatcactatg ctgcctgaaa actggatagc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcgga aatccagatt cttcggtaca gaaacttatc ggataaaacg
     2281 gtttctttag attctacgtc ctagattccc gcctgcgcgg gaatgacggc ataggggttt
     2341 ccgttttccc gataaattac cacaacccaa aatcccgtca ttcccgtaaa agcgggaatc
     2401 cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt cattcccacg
     2461 caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc cgcgaaagcg
     2521 ggaatccaga ccgttcggct tcagtttttt ggtttcgggc aacttctgca tcgtcattcc
     2581 cacacaggtg ggaatctagg tctgtccgca cgggaactta tgcgccgtca ttcccgcgaa
     2641 agcgggaatc cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt
     2701 cattcccaca caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc
     2761 cgcgaaagcg ggaatccaga cccttcggca cagaaactta tcggataaaa cggttgcttt
     2821 agattctacg tcctagattc ccgctttcgc gggaatgacg gcatagaggt ttctgttttc
     2881 ccgataaatt cctgttactt ttcgttgcta gattcccgct ttcgcgggaa tgatgaatga
     2941 cggtgtaaaa gtaactcgaa atccaaaaag ccataccgcc cggatttttg ccgatcggta
     3001 tggctttttg cttcaaaccg ccttagcgga tgtcgacata cgcgccggaa tgcaggtcac
     3061 gcaacaggtt gacggttgcc tgttcggctt tttgttggaa gatgtattgc cgcacggaat
     3121 tgcggatacg ttcctgaggt gtgccggcat cgcgcacttc gttcaatttg atgatatgcc
     3181 agccgaattg ggtgcggacg ggcgcgccga cctgtccggg tttgagcgcg tggacggctt
     3241 cttcaaaggc gggaaccatc acgccgtcgg caaaccagcc caaatctccg ccgttgcccg
     3301 cgctcgcgtc ttgcgaatat tggcgcgcta ggctggaaaa gtctgtgccg ctgcgggcct
     3361 ctccgtagat tttgcggatg gtgctttccg cgccgacggc ggcgttttcg ctgtcggctt
     3421 taatcaggat gtgttgggcg cggtattggc gcaacggtgc gccttcgggc agggtgatgc
     3481 cttgtttttg cgcctgctcg aggaaggcat cgatttcagc ttcgctcacg cggctgttct
     3541 gcatcactgc ctgctggcgg actttttcgg caatgatgtt gtcggcaaaa tcgcggcgtt
     3601 gggcggggct gaggtttttg agggcggaat tttttgcgac gacggcatcg atttccgctt
     3661 cgcttgcttg aatgttgcgg cgtttgcccg cctgtacaat cagggattgg ttgacaagct
     3721 gcatcagcac ctgtcgggac agctcggatt cgcttatctg cgcgtctttg ggcaggttgg
     3781 ctttggcttc ggcaacggct tcggcaagcc ggcggcgcgt gatgacttcg ttgtcggcaa
     3841 cggcggcaat gccgtctgaa aagcggatac cgccctgctg ttgtgcgggt gcggcttctt
     3901 tttgcgccgt ggcggcaact ttggcaactt tggcagcttt ggcagctttg gcagctttgg
     3961 cggatgcggt ttttgccttt tgcggtgcgg cgtggacatc ggctgctgcc agcaatgcgg
     4021 cggcaatcat cagggctttg attttcatca ttctttcctt atcgtttgaa tcctgccttt
     4081 gatggcgggt ttggggttgt cggtggaatg ccgtcttgtg cgggaacggt gctcctgccg
     4141 ggtttccgac ggtcagggcc gtttgttgcg tccggcggaa agagagtggg cggggatata
     4201 gccgggaacg gcgacatcca tcctgtctgc ggggtttctg ccgacactgc tgaggtcttt
     4261 caactgaagt gagaaaaaga cagcgttttt gtaggtgttt tcgccggtaa cgtagcgttg
     4321 ggcgtacacg cccgcgcccc agcagccgca actgcttttg tattccgcac ccgccagcac
     4381 ctctatcggt tttttggctt caaaaccgta gttgtaacgg acgacggccg acaggttgcg
     4441 cgtcagcggc cattgtgcgg acaggtcgag ctggctgagt ttgtcgtaaa aataggaacc
     4501 gtcggacttc aggtagattt tttcgttgcg cccgtatttg tagcgggcgt tcagcacttt
     4561 gccctgtgcg ggacggtagc ttgcaccgac ggcgtagttc tcggcgcgtt tgtcgttttg
     4621 gttgtagtgg atgctgctgt cgaggatgaa gcggctgccg atgccgctgg aggcgaatgc
     4681 cacccagtcg gaacggctgc gcggtttttt gccgacactg ccgtcaagca tgactgcgtc
     4741 gtttttgaag tagaatttct gcccgatgcc ggcgcggaaa cgctcttccc ccgtcgcgcc
     4801 gtccaaaata cggctttgca cggcggcgga aaggctgttt gcggtgttaa tcctgtcgtt
     4861 gccgtaatag aggttttcac gaaaaagctg cccgtagccg aagctgcttt ccgacgaatc
     4921 gaaattgggc aggtcgtttt gggatttggc aggaatatag ttgtagaaca ggcgcggctc
     4981 gagggtttgc aggactccgc cgccgaacat ccgcgtattg cgttcgaagg tcatgccgct
     5041 gtcgatgttg acgatgggca gagtgcggct gacgcgtcgg gcttcttggc tgccgaagcg
     5101 gttgaggctg taataggtgg cgtgcagtcc gagtttggga cggacgtaac cccagctgtt
     5161 gctgaaatcc catttgatgt cgggatagac gacgaggcgg ctgccgtctt ggcggctgtc
     5221 gtggctgaag cgggtaaatt gggcggacac gccgatttgc gccctgccgg tgtttttgcg
     5281 ccaatcggcg gaaaggcgcg gcatcagggc atacggtttg tctttgtagc cgctttggtt
     5341 tgccagcgtc tggtatttca gaaccgaaag gccggcattc aggctgccgc ccgccgccct
     5401 gccgccataa tccagccata cacggcggtt gaggttgacg ttgccggcga tttctttgtt
     5461 gccgtaaaag tcgcggtagt agccgctgtc ggagacttgg ttgaaatcga cacccgcctg
     5521 aagcgtgtcg gaaatgtcgt gccggtgctg ccatttcgcc tgatagcggt tattcctgcc
     5581 gcttttcttg tcgtgcggca gccaggtcag gtcggactgg ccggcataat ccggccgcag
     5641 gtagcgtacc tgcccgtcaa agaccgcgcc gcgttcgccg atcacgccgg gcgcgaacgt
     5701 ggcatcgaga ttgggggcaa ggttgaaata atagggaacg gaaagggaaa cgccgtccga
     5761 accggcggac agtgagggaa cgagcaggcc gcttttgcgg ttgccgtcaa gcgggaagtc
     5821 cgcccaaggg gtgtagaaaa tgggaacgcc gccgaacacg aaggcggcgt gtttggcaac
     5881 gcctatgcct ttttcccgat cggcttcgac ggaagcggcc ttgacatacc agccggcatc
     5941 gccggcggaa caggtgttga attgggtttc cgtcagtttg taatgccctt cgcccaacat
     6001 ttcggcggtg cggctgacgc tttgcagccg ccgtccgccg tgttcggttt ccatacggac
     6061 gttgtgcgct tccccggtct gctgctcgag attgtaggtc agggtttcgc cccgaatcag
     6121 cgtaccgtcc tgttggaggg cgaaccggtc gcctgcggta acggtgtcgc ccgactggtc
     6181 gtaatccgcc caatcggcat tgagggtcgt ccgattgcgt tcgacgacga cgttgccttc
     6241 ggcacgcacc tgcacctgcg actgtccttc catcctgtcg gcaacaatgc gcgtatagtc
     6301 ttcggggatg gatgcttcgc cgctgccttg gacggcggct tcggttctct cggggctgcc
     6361 gctttcgtta ctgcaaaaca ggcaggtcga accgaggctc aggctggtag gctgtatggg
     6421 ttcggacacg ctccgaacgc ttcctccggc ggtcggattg tccgtttcct ccgccgcaac
     6481 ggcatcggcg gcggcgcaat gcgtgccgaa acagaagccc aatgccagca ccagtggttt
     6541 gagtgaaaat aaacgagcca aaatcgcccc tcaagtcggt ttgccagtta gaatagcgtt
     6601 tattgtaacc tgaaatgctt tagtactgtt atgcaacggc aaaccgaact gaaaaattgg
     6661 cttcagacca tctatcccga acgggacttc gatctgtcct tcgcggcggc ggatgctgat
     6721 ttccgccgct atttccgtgc aacgttttca gacggcggca gcgtcgtctg tatggacgcg
     6781 ccgcccgaca agatgagtgt cgcgccttat ttaaaagtgc agaaactgtt tgacatggtc
     6841 aatgtgccgc agatattgca cgcggacacg gatttggggt ttgtggtatt gaacgacttg
     6901 ggcaatacga cgtttttgac cgcgatgctt caggaacagg gcgaaacggc gcacaaagcc
     6961 ctgcttttgg aggccatcgg cgagttggtc gaattgcaga aggcgagccg taaaggggtt
     7021 ttgcccgaat atgaccgtga aacgatgttg cgcgaaatca acctgttccc ggaatggttt
     7081 gtcgcaaaag aattggggcg cgaattaaca ttcaaacaac gccaactttg gcagcaaacc
     7141 gtcgatacgc tgctgccgcc cctgttggcg cagcccaaag tctatgtgca ccgcgacttt
     7201 atcgtccgca acctgatgct gacgcgcggc agaccgggcg ttttagactt ccaagacgcg
     7261 ctttacggcc cgatttccta cgatttggtg tcgctgttgc gcgatgcctt tatcgaatgg
     7321 gaagaagaat ttgtcttgga cttggttatc cgctactggg aaaaggcgcg ggctaccggc
     7381 ttgcccgtcc ccgaggcgtt tgacgagttt taccgctggt tcgaatggat gggcgtgcag
     7441 cggcacttga aggttgcagg catcttcgca cgcctgtact accgcgacgg caaagacaaa
     7501 taccgtcccg aaatcccgcg tttcctcaat tacctgcgcc gcgtatcgcg ccgttatgcc
     7561 gaactcgccc cgctctacgc gctcttggtc gaactggtcg gcgatgaaga actggaaacg
     7621 ggctttacgt tttaaaccca atcaaaatgc cgtctgaaaa ccaagtttca gacggcattt
     7681 tccaaacggg cttagtgcgc ggctttttgt tcttcgcgta ctttgtccgc caaaaggtcg
     7741 atggtgttca taccggactc ccagtcggca aattcgactt tatatttgcc gccgacgata
     7801 accgtgggcg taccgtcgat ttggaaggtt tcggtcagct cctgcatttt gtcggcgcgc
     7861 gcctggcttt cgggagattc gtaagcggca aggacttttt tgccgtcaaa ggcggtttgt
     7921 tcgcccagcc attttttgag gacttccggc tcttgcagct tgattttttg gttgaccatc
     7981 gcatcgaaaa tatggctgtt cgccacatct ttgctgtcgg cggcagccat atcgacggcg
     8041 gcggcgaggc gtgcgagcgt cagcatttct ttctgccaga cgacgtgttc ggtacgcagg
     8101 tacatatcgt ctttaaaaga cttggcgtgt ttgcttaaaa caggttcgag gtgggcgcag
     8161 tgcggacaga aatagccgaa aaactcaagg acttcgactt tgcctgcctg ctgttgggga
     8221 atcgggttgg caaggacagt atagttttgc ccttcgacca gccctgccgg ggcggcggct
     8281 gccgaagcgg caggcgcgct gtcggcgggg acgctggttt ggactttgct gtcgcacgcg
     8341 gcaagtgcga acagggcggc aacgccgagg gcgaggtgtt tggatttcat acggctctcc
     8401 gtgatgttgg aaacagatgt tggaaatcaa tcggaatccg gctattttat tgcatttccg
     8461 cgtgttgata cagtttgcct ccggaaaagg acgttttcgt ttggggaaac cgcttcggac
     8521 ggcatcaaac ccgatgccgt ctgaagcggt ttctgtcgta caatacgcgc cgtcgcccca
     8581 gacggatacg gctgttgggg aactaatgat gttccaacac acaggacggc acataaagca
     8641 ccgccctatg cgttgccctg gttcggaagg gttacacccc tttcaaatat agtggattaa
     8701 atttaaacca gtacagcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg
     8761 tcgccttgtc ctgatttaaa tttaatccac tatcaaagtc tgatcctgcc gccctaaagg
     8821 gcggggtttc aaccgaaaag gaaatacgat gaatatgctg ggagctttgg taaaagtcgg
     8881 cagcctgacg atggtgtcgc gcgttttggg atttgtgcgc gatacggtca ttgcgcgcgc
     8941 attcggcgca ggcatggcga cggatgcgtt ctttgtcgcg ttcaaactgc ccaacctgct
     9001 tcgccgcgtg tttgcggagg gggcgtttgc ccaagcgttt gtgccgattt tggcggaata
     9061 taaggaaacg cgttctaaag aggcgacgga ggcttttatc cgccatgtgg cggggatgct
     9121 gtcgtttgta ctggtcatcg ttaccgcgct gggcatactt gccgcgcctt gggtgattta
     9181 tgtttccgca cccggttttg ccaaagatgc cgacaaattt cagctctcta tcgatttgct
     9241 gcggattacg tttccttata tcttattgat ttcactttcc tcttttgtcg gctcggtact
     9301 caattcctat cataaattca gcattcctgc gtttacgccc acgttcctga acgtgtcgtt
     9361 tatcgtattc gcgctgtttt tcgtgccgta tttcgatcct cccgttaccg cgctggcttg
     9421 ggcggttttt gtcggcggca ttttgcaact cggcttccaa ctgccctggc tggcgaaact
     9481 gggttttttg aaactgccca aactgagttt caaagatgcg gcggtcaacc gcgtgatgaa
     9541 acagatggcg cctgcgattt tgggcgtgag cgtggcgcag atttctttgg tgatcaacac
     9601 gattttcgcg tcttatctgc aatcgggcag cgtttcatgg atgtattacg ccgaccgcat
     9661 gatggaactg cccggcggcg tgctgggggc ggcactcggt acgattttgc tgccgacttt
     9721 gtccaaacac tcggcaaacc aagatacgga acagttttcc gccctgctcg actggggttt
     9781 gcgcctgtgc atgctgctga cgctgccggc ggcggtcgga atggcggtgt tgtcgttccc
     9841 gctggtggca accttgttta tgtaccgtga attcacgctg tttgacgcgc agatgacgca
     9901 acacgcgctg attgcctatt ctttcggttt aatcggtttg atcatgatta aagtgttggc
     9961 gcccggcttc tatgcgcggc aaaacatcaa aacgcccgtc aaaatcgcca tcttcacgct
    10021 catctgcacg cagttgatga accttgcctt tatcggcccg ttgaaacacg ccgggctttc
    10081 gctcgccatc ggtttgggcg cgtgcatcaa tgccggattg ttgttcttcc tgttacgcag
    10141 acacggcatt taccggcccg gcaagggttg ggcggcgttc ttggcgaaaa tgctgctctc
    10201 gctcgccgtg atgggcggcg gactgtgggc ggcgcaggct tgcctgccgt ttgaatgggt
    10261 acgcgccggc ggaatgcgga aagcggggca gctctgcatc ctgattgccg tcggcggcgg
    10321 actgtatttc gcatcactgg cggctttggg cttccgtccg cgccatttca aacgcgtgga
    10381 aaactgaccg atgccgatat cctttttcgg caaacgcttt tccacgccta tattcgggcg
    10441 cgcatccccc acgtccgcac cagccggacg ggctgccgca tcatcaatgc cgtctgaaaa
    10501 caagaaaacc gataccttat gattttgaca ccgccggaca cacccttttt cctccgcaac
    10561 ggcaatgccg acacgattgc cgccaaattc ctgcaacgct ccgcacctgc ataccgccgc
    10621 gagctgcttc ccgacagcac gggtaaaacc aaaaccgcct acgacttttc agacggcatt
    10681 tcgcccgatg cgccgctggt cgtgctgttt cacggtttgg agggcggcag tggcagccat
    10741 tacgcggtcg aactgatgct cgcggtacgc gatcggggtt ggaacggcgt agtcgtccat
    10801 ttccgcagct gcggcggcgt agcgaacacc gccccggtgt tctaccactt gggcgatacc
    10861 gccgaaattg cctttacttt ggacacgctc gccgcgcgtt accgtgaaat atacgccgtc
    10921 ggcgtatcgc tgggcggcaa cgcgctggca aaatatttgg gcgaacaggg cgaaaacgcg
    10981 ctgccgcaag ccgccgccgt catctccgca cccgtcgatg cagaggcggc aggcaaccgc
    11041 ttcgacagcg gcatcacacg gctgctctac acgcgctact tcctccgcac actgataccc
    11101 aaagcacggt cgctccaagg ttttcagacg gcatttgccg cagggtgcaa aacactgggc
    11161 gagtttgacg accgtttcac cgcaccgctg cacggctttg ccgatcggca cgactactac
    11221 cgccaaactt cctgcaaacc gctgctcaaa cacgttgcca aaccgctgct cctgctcaat
    11281 gccgtcaacg accccttcct gccgcccgaa gcgctgcccc gcgcagacga agtgtccgaa
    11341 gccgttaccc tgttccagcc gacacacggt ggtcatgtcg gctttgtcgg cagcaccggc
    11401 ggcaggctgc acctgcaatg gttgccgcag accgtcctgt cctatttcga cagcttccgc
    11461 acaaacaggc gttaacggtt tgatgctaat attccccctt tccccagacg aatacggaac
    11521 acgacatgac cgacatcctc aataaaatcc ttaccgccaa ggcacaggaa gttgccgccc
    11581 aaaaagccgc cgtcaacgcc gaacacatcc gcgcacttgc cgcagaagcc gcgcccgtcc
    11641 gcagctttat cggttcgata cgcggcaaac accgcctcaa cctgcccgcc gtcattgccg
    11701 aaatcaaaaa ggcaagcccg agcaaagggt taatccgtcc ggacttccga ccgcccgaga
    11761 ttgcacgcgc ctatgaaaac gccggggcgg cgtgtttgtc cgtactgacc gacgaaccct
    11821 atttccaagg ttcgcccgaa tacctcaaac aggcgcgcga agccgtatcg ctgcccgtgc
    11881 tgcgcaaaga cttcatcatc gacgaatacc aggtttatca ggcgcgcgcc tggggggcgg
    11941 atgccgtcct gctgattgcc gccgcactgg aacgggaaca attggaacgc tttgaagcgg
    12001 tggcgcacga attgggcatg accgtcctgc tcgagctgca cgatgaaagc gaattggaaa
    12061 aatgccgcaa cctgaccacg ccgttgtggg gtgtaaacaa ccgcaacctg aggacttttg
    12121 aagtctccct cgaccaaacc ctgtcgctgc tgcccgcgct ggaaggcaaa accgtcgtta
    12181 ccgaaagcgg cattacaggc aaggcggatg tggaatttat gcaatcgcgc ggcgtgcata
    12241 ccttcctgat cggcgaaacg tttatgcgtg ccgacaatat tgaggcggaa gtaggcaaac
    12301 tcttctaaat cccgatttca gacggcattc cctttcttta accgacacac cctttaccgg
    12361 acacggaacc atcatgcacc gaccgaccgc caaacagatt ctgcacgaag tatttggtta
    12421 tcccgaattt cgcggcaggc aggaggctgt catcaatact ttggcaggcg gcgggagttt
    12481 gacggtgctg atgccgacgg gcgggggcaa gtctttgtgt taccagattc ccgcgctgat
    12541 gcgcgaaggc gtggcggttg tcgtatcgcc gctgattgcg ctgatgaacg accaagtggc
    12601 cagcctgcat gtggccggga ttgaagcagc ggcagtcaac agtggtacat cggcagatga
    12661 ggcgcgcgag attgccgaca agcttgccca aggccgtctg aagctgcttt atgtcgcacc
    12721 ggaacgccta gttaccgacc gctttttacg ttttctcgac caacaaaccg tcagcctgtt
    12781 tgccattgat gaggcgcatt gcgtcagcca gtggggacac gatttccgcc ccgaatacca
    12841 acagctcggc atgcttgccg aacgctatcc gaacgtcccg cgcatcgccc tgaccgctac
    12901 cgccgatgcc gccacgcgcg ccgacatcaa gcattatctg cacttggacg acgcgcccga
    12961 atttgtctcc agctttgacc gcccgaatat ttattatcag gttatcgaaa aaaacaacgg
    13021 caaaaaacaa ctgcttgaat tcatccgcaa agaaatgacg gggcaaagcg gcattgtgta
    13081 ttgcttaagc cgcaaaaagg ttgaagatgt ggcgcggttt ttgcgtgaaa acggattaaa
    13141 cgcgattccg tatcacgccg gtttgggtat ggacgtgcgc gaggaaaacc aacgccgctt
    13201 tacgcatgaa gacaatatta tcgtggtggc gaccgtggcg ttcggcatgg gcatagacaa
    13261 acccgacgtg cgctttgtcg cccatctcga tatgccccaa agtgtcgaac atttctatca
    13321 ggaatcgggg cgtgccggcc gggacgggct gcctgccgtg agctggctgt gttacggctt
    13381 gaacgattgg gtgttgctgc gcgaacggat tgccgaaggc aacagcgacg aggtgcaaaa
    13441 gcaaatcgaa atgcaaaaac ttgatgccat gctttctatc tgcgaaaccg ccgcctgccg
    13501 ccgcgtgctg ctgctcaaac atttcggcga agcatccgaa ccctgcggcc gttgcgacaa
    13561 ctgcctgcat ccgcctgtgc ggtttgacgg cacggtgttg gtgcaaaaat tactcagctg
    13621 cgtgtaccgc gccggacaac gttttgccgc cggttacatc accaaccttt tgcgcggcaa
    13681 aagcgatgat tggatacagc gcaacggcca tgacaagtta tccacattcg gcatcggcag
    13741 cgaacagacc gacaaggaat ggcgcagcgt catccgccag tgcatcagcc tcggctatct
    13801 caccgtcaat gtcgagcagc atcaagccct gcaactgacc gaagcggcaa aaaaagtcct
    13861 caagggcgaa accggagtga tgctgcgccc gctcaagcgc gacaagcccg ccgtccgcac
    13921 cctcaaagac aactggctgc gtaccgaacg cgaagaacgc ctgtggcagg cattgcgcgt
    13981 ttggcgtatg aaacaggcag aagccgaagg catccccgcc tatatgattt tcggcgacaa
    14041 aaccctgcgc gaccttgtcg aaaaaatgcc gcaagacctc aacggtctgc acgacatcta
    14101 cggtttgggc gaagccaaaa ccgaacgttt cggacacggc atactcgaag tctgccgcaa
    14161 cgccgccggc ttcagccgcg acgcggtcat ccgtccgcaa accgaacgcg aacaacaact
    14221 gcgtcaaaaa ctcgaagcct ggcggtatga acaggcaagg gcggaaaact gcgccctgca
    14281 ttccgtcctc tccgacgaaa gccttgccga tatgcttgcc gatacgcccg aaaccgaaac
    14341 cgacctcgaa ggcgtacacg gcttgggcag cgtacgcgcc gccaaatacg gacgggacat
    14401 cctcgccgtc tgccgtccgt tttcagacgg catcgatgaa gccgccaaac acaaacgccg
    14461 cctgatgcgc gccctgattc aatggtgcaa cgaaacggca aaacacgaac agtccgaacc
    14521 ctaccgcatt ctcagcaaag ccgccctgcg cgccattgcc gccaaacagc cggaaggttt
    14581 ggcggagctt gccgccgtat acggcgtagg cgaagaaaaa gccgcacgtt acggtgcggc
    14641 ggtgttggcg gtattggaac gaaatgccgt ctgaagcccg ttaccaagtt tcagacggca
    14701 ttgcctctat ttaaaaattc c
//
LOCUS       DAWFIT010000051        14336 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFIT010000051 DAWFIT010000000
VERSION     DAWFIT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.051"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1007..1342)
                     /locus_tag="WGQ85_001359"
     CDS             complement(1007..1342)
                     /locus_tag="WGQ85_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0750772.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(1423..3552)
                     /gene="dnaX"
                     /locus_tag="WGQ85_001360"
     CDS             complement(1423..3552)
                     /gene="dnaX"
                     /locus_tag="WGQ85_001360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0750773.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALRNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSVQTAEKEAAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(2324..2363)
                     /gene="dnaX"
                     /locus_tag="WGQ85_001360"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(3647..5623)
                     /gene="mutL"
                     /locus_tag="WGQ85_001361"
     CDS             complement(3647..5623)
                     /gene="mutL"
                     /locus_tag="WGQ85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0750774.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQKDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHEITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNVLLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5767..5952
                     /locus_tag="WGQ85_001362"
     CDS             5767..5952
                     /locus_tag="WGQ85_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0750775.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5963..6631
                     /locus_tag="WGQ85_001363"
     CDS             5963..6631
                     /locus_tag="WGQ85_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0750776.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            6631..7347
                     /locus_tag="WGQ85_001364"
     CDS             6631..7347
                     /locus_tag="WGQ85_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0750777.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWLSL
                     IQTYPGSPAAKRAAAAIRKR"
     gene            7395..7880
                     /gene="purE"
                     /locus_tag="WGQ85_001365"
     CDS             7395..7880
                     /gene="purE"
                     /locus_tag="WGQ85_001365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0750778.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(8063..9517)
                     /locus_tag="WGQ85_001366"
     CDS             complement(8063..9517)
                     /locus_tag="WGQ85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0750779.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9514..10215)
                     /locus_tag="WGQ85_001367"
     CDS             complement(9514..10215)
                     /locus_tag="WGQ85_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0750780.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(10212..10991)
                     /locus_tag="WGQ85_001368"
     CDS             complement(10212..10991)
                     /locus_tag="WGQ85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0750781.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            11276..12682
                     /locus_tag="WGQ85_001369"
     CDS             11276..12682
                     /locus_tag="WGQ85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0750782.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            12794..14320
                     /locus_tag="WGQ85_001370"
     CDS             12794..14320
                     /locus_tag="WGQ85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0750783.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2904 a         3864 c         4014 g         3554 t
ORIGIN      
        1 ttttgcccga cggggtgaaa aatacagttg ctacagcccg acctacgccc gctttgcctc
       61 tatcctgcgc ccttttatgt gcaacacttt gcacttgctg aacaaaattc aaacgaccct
      121 ttatatcaaa tgcaaaaaat atgccgtcat tcccgcgaag gcgggaatcc agacccctcg
      181 gcacggaaat ttatcgagta aaacggtttc tcagattcta cgttctagat tcccgcgttc
      241 gcgggaatga cggtcggggg tttccgtttt ttccgataga ttcccgtggt ttttcggtta
      301 ctagattccc gcgttcgcgg gaatgacggc ggcggggggt tctgtttttt ccgatagatt
      361 cctgtggttt ttcggttact ggattcccgc gttcgcggga atgacggggt gtaagtttct
      421 gctcccacgg ggctggattc ccgttttcac gggaatgacg aagtttcaga cggcatttaa
      481 gcggtacgga tgtgtaaata atggtagggt gggcttcagc ctgccgattc ccatcgattc
      541 tgccgttttt accgtttccg ccgaatcctg caaactgatg ccgtcattcc cgcgaaggcg
      601 ggaatccaga cccctcggca cggaaattta tcgagtaaaa cggtttctca gattctacgt
      661 tctagattcc cgcgttcgcg ggaatgacgg ggtgtaagtt tctgctccga cggggttgga
      721 ttcccgcttt cacgggaatg acgaagtttc agacggcatt taagcggtac ggatgtgtaa
      781 ataatggtag ggtgggcttc agcctgccga ttcccgctat tcttgccgtt tttgcgttct
      841 tatcattctc actgttttta ccgttcacgc cgaatcctgc aaactgatgc cgtcattccc
      901 gcgaaggcgg gaatccagac ccgtcggcac ggaaatttat cgagtaaaac ggtttcttag
      961 attctacgtt ctagattccc gcctgcgtgg gaatgacggt cggggatcag cggaagaagt
     1021 cgcccactcc gggggggagt ccttgggcga atgcgcccat tgttttgttt gcggtttctt
     1081 cggctttgcc acgggcggat ttgagggcgg cgaggatgag gtcttcaagc atttctttgt
     1141 cgtcggcggc ttcttgaatc aaatcggggc tgatgtcgat tttgcgtact tcgtgcgcgc
     1201 aggtcattgt gattttgacc aagccgttgc ctgcttcgcc ttcgatttcg gtttcggcaa
     1261 gtttggcttg cgcttttttc atattttcct gcatttgctg cgcctgtttc atcaggccgc
     1321 ctaatccggc ttttccgaac atactgaata ctcctgtttg tgtttaaatg aatgagggaa
     1381 acggcaatgc cgtccgaagg gttcgggcgg cattatatct gtttatggcc ggtttgccgc
     1441 caattccagt gattcgggct gccattgcgc gccgaatgct tggaggattt tttgtgcggc
     1501 ggggtcggct tcgagcaatg cttgtgcttt ttgcctgtcc tcggcttgga cgcgcttgtc
     1561 ctgcatcgcg ggggtttctc tgccggattc gtcctgccag tcttgggttt gcagggtgag
     1621 ttgcagcccg taggcttggg caagggtgtc gcggattttg tcgagacgtt ttttgtcggc
     1681 ggtggcgcgt gcttcggcgg tcattgccaa aaccatcagg ccggtgtcgg gatggtattc
     1741 cgtccacgcg gagtgttgcg ccggcatttg cgccgcgccg agtttgcggg cgaaatgccg
     1801 gacgatggct gcccagtttt cggtggaaaa ctcgggcggt ggggcggacg gtgtgttgtt
     1861 tccgccgatg ccgtctgctt ctgcttcttc gtccgcgcct ccgcctgcgg tatcggcagg
     1921 ggcggcgtgt tcccaatcgg gcggcgggat ttccgcgttg tcttctacgg ggcagtcatc
     1981 atccggaaag ctgtaaccgt tgaaaggttt tgcaggagct tcgtgcgcaa atgcttccgt
     2041 ttcaagggct tcattattcg gtgttgtctg aatggggttt tcagacggca cttcggacaa
     2101 gggggcattg gtttcgttgt cggctgcctt gttcttggaa acttggtttt cgggcggcgt
     2161 ttcggcttcg gatgccgtct gaatgctttt tgccgacgtt tgcgccgtgc cggctgcggc
     2221 ttgggcttcg tctggcgcgt cttcccaagg cggaacatcg ttgttttctt gattggaaac
     2281 gggttcggcg gttttgcctt cagacggcat tgccgctgcg gatgccgtct gaacggatgt
     2341 ttgggcggtt tccgcttcag ggcgcggttg gggctttttt gcggcggctt ccttttcggc
     2401 ggtttgtacc gatggggatt gaagttcggt attttcaatc acggcgtttg catcacacga
     2461 agctgccgcc aagggcgcaa acgccagcat acgcagcagg gtcatcataa agccggcgta
     2521 ttcgtcgggg gcgaggccca agtcgcgttt gccgtggacg gcgatttggt agtaaagctg
     2581 gatttgttcg ccacctatgg tttgggcgag gcggtgcaaa atatcggaat cggggtcgtc
     2641 gtgcgccaag gcattcggca ctgcctgtat cagggcgagt tgttgcagca gtatggcgag
     2701 ttcgcccaag gcgttgtcaa agccgacggc acacgccgcc atttcccgcg ctttggcggt
     2761 cagggctgcg ccgtcttggt tgatgatgcc tgtcagcagt tcgtaaaggt attgtttgtc
     2821 aaccgcgccg atcatttggc ggacatcgtt ttcggcaact ttgcccgaac ccagggcgat
     2881 ggcttggtcg agcaggctta aggcatcgcg catcgatccg gcggcggcgc gtcccaaaag
     2941 ttgcagggcg gcgggttcgt aggcgatttt ttcgctgtcg aggacgtggg cgaggtggtc
     3001 ggcaacctgc tgcgcggtca tattgcgcaa gacgaattgc aggcagcggc ttaatacggt
     3061 aacggggact ttgtgcggat cggtcgtggc aaggatgaat ttgacgtgtt cgggcggctc
     3121 ttccagcgtt ttgagcatag cgttgaacgc gcttttggaa agcatatgca cttcgtcgat
     3181 gatatagact ttgtatttgc cggcagtcgg cgcgtattgg gcgttttcca agacttcgcg
     3241 gatgttgtcg atgcctgtgt tggaggcggc gtcgatttcg agcaggtcga catagcgtcc
     3301 ggcatcgatt tgggtgcagc tttggcacac gccgcaaggt tcgccgtgtt gcgcgttttc
     3361 gcagttgagg cttttggcaa ggatgcgggc gatggtggtt ttacctacgc cgcgcgtacc
     3421 ggtcagcagg taggcgtggt gcagcctgcc ttcgtccagc gcgttgcgca aggctttgac
     3481 gacgtgttcc tgaccgacta agtcggcaaa ggttttcgga cgccattttc gggcgagaac
     3541 ttgataggcc atgtttttct cttggtttcg gtcgtgatgt ttctgtcggt gcttcggaat
     3601 gccgtctgaa cggcggtctc gggcggcgta ttctagcact ttcggcttat tgtccgcgca
     3661 agaacagtgc gtccaattct ttcaaagtca gtttgaccca cgtcggcctg ccgtggttgc
     3721 actggttgct gcgcggcgtg ttttccatat cgcgcagaag gacgttcatt tcgggcaggg
     3781 taagccggcg gccggcgcgg atcgagccgt ggcaggacat ggtggcgagg atgcggtttt
     3841 cgtgttcctc gatggtttgg ctgctgccga cttgggcgag ttcgcccaat acgtcgcggg
     3901 cgagggagac gacatcggat ttgcccaaca ttgcgggtgc ggcgcggacg gcgagggtgt
     3961 tgccgcccat atcggataat tccagcccga agcctgccag cgtttcggca taatcggcaa
     4021 gggcggcgca ttcttcgtgg gacgcggcaa aggttacggg aataagcagg cgttggcttt
     4081 gcagcctgcc gttttcctga cgctggcgtt tcattttctc gtagttgacg cgttcggcag
     4141 cggcgtgcat atcgatgagc agcaggctgt cttcggcttg ggcaagaatg tagatgccaa
     4201 gtaattgggc aatggcgaaa ccgagcggcg gcagttcgga ttgggagggg atgccgtctg
     4261 aaagcggcgt atctgtttgg ggagcaggcg tttcagacgg catattgccg aaacgtgcct
     4321 gctcgaatcg gcttaactca aggtcgatgt cgtcggtttt tttgtaaagc tcggcatagg
     4381 tgttcattgc cgcgcggctt tcgcgcaggg acaggctgcg ttgttgcggc gcacgcgcgg
     4441 cttggtaggg cataggcgcg gttttgcctg atgcaccaaa ggcattgcgc gtatcgggtt
     4501 tgttgcctgt cgggtagtcg gatgcgcgat taaacagatt ttcgctgtcg ttttcagacg
     4561 gcattggtgc aggcgtaact cccgtgattt cgtgcaacac ttcgcccgcg ttgccgacgc
     4621 tttcggtcag gtcggcgcgc gtgtcggcaa gggctttgtt gagcgtgtgg aacacgagtt
     4681 ggtgtacctg ctgactgtcg cggaagcgga tttcggtttt ggtcgggtgg acgttgacat
     4741 ctacggcttc gggcggcagg tcgagaaaga ggacgaaagc aggtgtcagc gcgttgtgca
     4801 atacgtcgcg gtatgcctgc ttgacagcat ggagcatgac tttgtcgcgg acaaaacgat
     4861 ggttgacaaa gcagtattgt ttgtcggttt tgcctttggc gaaagtcggc ttggcaatcg
     4921 cgccatagag ccgcaatgcg ccattgccgc tgtcgatttc caaggatgcc gtctgaaagt
     4981 catcgccgac aatggcggca atccgttcat gcaggctttg tgcagggagt ttgaacactt
     5041 gtttgccgtc gcgtttgagt gagaaggcga tgtgcggatg cgccagagca agtcgttcga
     5101 gcatggtggc gcagtgggcg tattcggtgt tttccgattt gaggaacttg cgccgcgcgg
     5161 gggtattgaa gaagagttcg gcagcttcga tggtggtgcc gacggggtgg gcggctgccg
     5221 tagggctgct gagtttgccg tcttcggctt tgacttgggt cgcgtgcgaa ctgtcttttt
     5281 gacggctggt cagggtcagg cggctgacgg aggcgatgct tgccaagcct tcgccgcgaa
     5341 agcccatgct ggcaacgtgt tccaaatcgt ttaaggtttt gattttgctg gtggcgtggc
     5401 ggtggagcgc gagttcgatg tcgtccggat ggataccgct gccgttgtcg ctgacgcgaa
     5461 tcaggcggat gccgccgccc gccagttcga cttcaattgc tgttgcgcct gcatcgatac
     5521 tgttttcaac gatttctttc agggcgttgg cagggcgttc gaccacttcg ccggcagcga
     5581 tttggttgac aagatggtcg ggcagggcgg cgattcggga cataaggcgg gcttccgttg
     5641 caaaaaacgt cttattctat aataaaaccc cttatctttc tgcccgtatt cttgataaaa
     5701 gccgtttatc ccgtttggaa aataccagta taatcacgcc atattccgta aaattggagc
     5761 acaaagatgt atcactacca atccgatgcc acacaattcc tcaaccgcct gattgaagaa
     5821 aaacctgagt tggaacagca gcgtttggaa aacagggggc ttttgtggga tgtcgaactc
     5881 aatcccgaag aacaggaaaa cttcgaggcg gcaaaagtag cgaaaaaacc ttatacctat
     5941 taccaagact gaacccgccg aaatgacgac ccatctgtcc aatgtcgcac cggacctgca
     6001 aaactatctg aacagcatcg gcgaacccga acatcccgtt ttgacgcggc tgcgtgagaa
     6061 gaccgagcat caccgtatgg gcaaaatggc gattgcgcgc gaacaggcgg cggttttggt
     6121 ttggctggca aagctgattc gcgcggaaaa atatctggaa gtcggcgtgt ttaccggata
     6181 cagcagcacc gcgcttgcat tggcgttgcc agaacacggg cggattaccg cctgcgacat
     6241 caatgtaacc tttaccgata cggcgcgtca ggtttggaac gaggcaggtg tggcacataa
     6301 aatcagcctg cacctgcaac ccgcattgct gacattggat gatttgattg tacagggtga
     6361 agccggaagc tacgatttgg cactgataga cgcagacaaa ccgcccacgc cgcaatattt
     6421 cgagcgttgc ctcaaactcg tccggcaagg cggcatcatt gccattgaca atattttgtt
     6481 gaacggaagg gtgatgcgcg aagcggcttc cgacgcgccg cccagtgtcg gcatcctcaa
     6541 agatttcaat caaaacctgc cgaacgaccc gcgcatcgtc cccatcaccc tgcccgtcgg
     6601 cgacggtttg accctgcttc tgaaaaaata atgaagacca aattaccgct ttttatcatt
     6661 tggctgtccg tatccgccgc ctgttcttcc cctgtttccc gcaatattca ggatatgcgg
     6721 ctcgaaccgc aggcagaggc aggtagttcg gacgctattc cctatcccgt tcccactctg
     6781 caagaccgtt tggattatct ggaaggcaca ctcgtccgcc tgtcgaacga agtggaaacc
     6841 ttaaacggca aagtcaaagc actggagcat gcgaaaacac acccttccag cagggcatac
     6901 gtccaaaaac tcgacgaccg caagttgaaa gagcattacc tcaataccga aggcggcagc
     6961 gcatccgcac ataccgtcga aaccgcacaa aacctctaca atcaggcact caaacactat
     7021 aaaagcggca ggttttctgc cgctgcctcc ctgttgaaag gcgcggacgg aggcgacggc
     7081 ggcagcatcg cgcaacgcag tatgtacctg ttgctgcaaa gcagggcgcg tatgggcaac
     7141 tgcgaatccg tcatcgaaat cggagggcgt tacgccaacc gtttcaaaga cagcccaacc
     7201 gcgcccgaag ccatgttcaa aatcggcgaa tgccaataca ggcttcagca aaaagacatt
     7261 gcaagggcga cttggctcag cctgatacag acctatcccg gcagcccggc ggcaaaacgc
     7321 gccgccgcag ccatacgcaa acgatagttt ttccattttt ccgcttttta ccccatccgc
     7381 accaaggagg atttatgata caaatcggca tcatcatggg cagcaacagc gattggcccg
     7441 ttatgcggca ggcagcgcag tttcttgaag agttcggcgt agaatatgag gcgcgcgttg
     7501 tttccgcaca ccgcaccccg gatttgatgt tccaatacgc cgaaaccgca cgggcgcgcg
     7561 gcatcaaagc catcatcgcg ggcgcgggcg gtgcggcgca tttacccggt atggttgccg
     7621 ccaagaccac cgtccccgtt ttgggcgtac ccgtccccag caaatacctg cgcggcgaag
     7681 attcgctttt atcgattgta caaatgccca aaggcgtacc cgtcgccaca ttcgccatcg
     7741 gcgaggcagg cgcggcaaat gccgcattgt tcgccgtttc catgctggcc aacgaaaacc
     7801 ccgaactggc gcaaaaactg gcagacttcc gagccaaaca agaacagact gttttgaata
     7861 tggaattgga acagatttaa aacccgccgt ccgcgaatat atagtggatt aaatttaaac
     7921 cagtacggcg ttacctcgcc ttgccgtact atttgtactg tctgcggctt cgtctccttg
     7981 tcctgatttt tgttaatcca ctatacaaat gccgtctgaa gccctgtttc gggtttcaga
     8041 cggcattttt gcgaacaact ttttatgcct gttctttttg gcgcattttt tctgccatca
     8101 gttcgtgcag ggttttcttc gccggtttca tcggcacgcg gttttgcgtc caacccaact
     8161 gtttgcgcgg ggtcaggttg cggaacttgg tggctgccca accgaaggcg cggtaggttt
     8221 tgctgccgct gaaaataccg ttgaatgtgc gccacgccat ttgttcgccg aaggtatgcg
     8281 atgcgccttg ccctcggatg gggtgcggca cggtttcggt cggcgaacgt tgcgcttcaa
     8341 cgcgcaaacg ctgcatttgt tcggtaatcg ggatgcgtac cggacagact tccacacacg
     8401 caccgcacat ggtacaggcg gtcggcagat cgcgggtggc atcaagaccc aacaggtgag
     8461 gggaaataat ctcgccaatc gggccgggat aggttgtgcc gtatgccgcg ccgccgatgc
     8521 gggtataaac cgggcaatgg ttcatacacg cgccgcaacg gatacattgc agggtgcggc
     8581 gcatttggtc ttcggcataa gcctggctgc ggccgttgtc gagcagaacc aagtgcattt
     8641 cttgcggacc gtctaattcc tcactgcggc gcggaccggt aatcatgttg aaataagtgg
     8701 taatgttctg accgatggca gaacgcggca gcagactgta caagggtgga atatccgaca
     8761 gcttggcaac tactttttca atgccggtaa tggcgatatg cacgggcgga acggtggtac
     8821 tcaagcgacc gttgccttcg ttttccacca gacacagcgt acctgtttca gcaacggcga
     8881 agttcacgcc actcaaaccg acatcggcag tgctgtaaat atcgcgcagg gctttgcggg
     8941 cgaagccggt cagttggtcc acatcgtctg taagcggtgt gccgaggttt tggtggaaca
     9001 gttcgctcac ctgttctttg gttttatgga ttgcaggcat cacgatatgg gtcggttttt
     9061 cgcctgccat ttggacgatg aactcgccca agtcgctttc taccgcttta atgccttttg
     9121 cttcaagata atggttcagc tcgatttcct cgctgaccat cgatttgcct ttgaccatca
     9181 gcttgccgtt tttggctgtg atgatgtcgt ggataatttg gcaggcttcg gtcggggttt
     9241 ctgcccagtg cactttcacg cccaacttag tcaggttttc ttccagctgc tccagcaggg
     9301 ctggcaattt agacaatgag cgctgacgga cgtgttcgca caaatcgcgc aggctttgca
     9361 gctcttcttc gtcggtcaaa acggctttgc gtttggtcat cagcatatcc atcgcggtac
     9421 gcaggctttt gcgcaaaggc ttgtcttgaa gggaaattgc ggcgttttgc ttgaaagttt
     9481 ccggcttcat gtgaaatttg atggtttgcg tagtcatgcg ttttcctcca aatcggcagg
     9541 ggaaatgtgg tcgggcagga tggcgaggat gaccaaatcg cgcgggccgt gcgcgccgta
     9601 agcaagcgtc agttggatgt ctgcggtttt ggacgggccg gaaatcagga atacattggt
     9661 cggcataccg ttttccacca gtttttcgcc ttcgacggca ttatgaaact cgttgtacat
     9721 cttggacgta tcgaacaggc agaaatgcac gggcggaacg aggcttaaag tacgcggttc
     9781 ttcggggctg gaaaacagca tcagcgtgcc ggtgcgggcg atgccgcatt gcgcgccgct
     9841 gaagcccgca tcgatgttcg tgaaaaactc ggttttccaa gtatcgattt cgcgctcgaa
     9901 ggcaatcggt tcgatattgc tgtccgccaa tgcggcacgg gcaatttgtc cgtgttcggt
     9961 cgccaagggc agcaggatgt ttttcaaacc cttgccttct gccgcttcgc ggaaaacctg
    10021 catccaattg cttttcgtca cccaataaat ttcggttttg accgcacgca tagcggcagc
    10081 ccaatgtttc agacgctcaa cttcgctgcc ccaagaaaca cccatttcac ggtaataatc
    10141 aaaaaccgca ggttcttcca tcggcaatgc gtcggctttt ttcagttttg ccaaaatatt
    10201 ttcacgcgcg ctcatgcttt gcctccggtg cgttccaaca agaaggatgc gatatgtttc
    10261 ggacgcggca tatccggctc gtccttggcg attttgccgc cgatgttcat catacagccg
    10321 cagtccgcgc tgatgatttc ggttgcgccg gtttctttca gcgcggcgac tttgtctgtt
    10381 accattgcgc cggaaatatc ggcttgtttg acggagaatg tgccgccgaa gccgcaacat
    10441 tcgctttcgt ggtcgtggac gatgcgttcg acgttttcca taccgtcaat cagttgccag
    10501 cctgaaagat ggacattcat ttcgcggcgg gcggcgcagg aagtgtgaac ggcgactttg
    10561 agcggttcgc ccttgtcttc gggtttgaaa ccgatggcaa gcaggaaatg ggtaaactcg
    10621 atgatgcggc cggcgcaatc cacagccctt tcctcgtact cgctgccttt aaacagcgtc
    10681 ggccagtggt gtttcatcat gccgccgcac gagccggacg gcacgacgat cggccagttt
    10741 tcagggaaaa ggtcgagttg tgctttggcg acatcgaagg cttcggtcgg atggccggat
    10801 gaataggcag gctggccgca gcagctttgc gccatcggga aatggacgcg tatgccctgc
    10861 tgctcgatta gggtaatggc atccatgcct gcttcgggca taaaaagatc aaggacgcaa
    10921 gtgccgaaaa aatagacatc ggtcggattg ctgtcgtatc ggatgatttt tgggggaatg
    10981 gttgcgctca tgttttttga taacgggaaa cccgtttttc tctgtagaaa ggtaagcgtt
    11041 tactttaagt aattgactgt tgcgggtcaa gtctaatttt aaaaaataat ccggtttttc
    11101 ttacaaactg cccccataac gcttactgta ccttaatctg atggttttcg ataataatta
    11161 tcattacaat gtaatgccgg ttcgtttgct tgtgaacatt caagatgccg actctgacgg
    11221 cattcagaca gcatctgaaa acaataacgg cacaaggatg gggtacaatc gccctatgga
    11281 aaaaaacacc ctatccgccc gcgcaccatc cccttggctg ccgcttctgc tggcaattgc
    11341 catttttatg cagatgttgg atgcgaccat tttaaatacc gcactgcctg aaattgccgc
    11401 cgacctgaat gagtcgcctc tggatatgca actggcagtt atttcctaca cgctgacggt
    11461 tgccctgctg attcctttga gcggttattt ggcggacagg ttcggaacga aaaaagtctt
    11521 tttcggttcg attgccgttt ttatgctcgg atcggcattg tgcgccgcat cgggttcgct
    11581 gtttgaattg acgctttccc gtgtcgttca gggcatcggc ggttcgatgc tggttccgat
    11641 accgcgtctg accatcttgc gtgtgtacga caagtccaag ctgctcaatg ccatcaatta
    11701 tgcggttatg cccgcattaa tcgggccggt tttagggcct ttggcgggcg gttatttggt
    11761 cgaatacgct tcgtggcact ggattttcct gctcaacctg cccatcggtc tgctgggttt
    11821 catattggga cgcaacatca tgcccgatat taaaggcagt aatatctctt tagacttcaa
    11881 aggttatctg attttttctg ccgccgcgtg cctcttgtta ctttcggcag aaagcctgtc
    11941 gcacgcgctg cctccgtatt ttgcactgtt gccgctgtgc ggcggactgc tgtttgcacg
    12001 ccgttatttc cgacatatga aaaccgcgtc caaaccgatt tattccgccg acctgtttct
    12061 gatacgcact ttccgtctgg gactggcggg caatctgttc agccgtctcg gcatcagctc
    12121 gattcctttt ctgatgcccc tgatgtttca aatcgctttc ggcttcggcg caagcctgtc
    12181 gggttggctg gtcgcacccg tcgccctgtc ttcgctgctg gtcaaaccgc tgattgcacc
    12241 gctcatgaaa cgtttcggct accgcacggt actgctttgg aacaccaagc tgcttgccgc
    12301 cttcatcatg ctgctcgccc tgcctgacgg aaactcgccg ctgtggattt gggttttcct
    12361 ctcgctggcg atcggcgcgt gcaactccct acagttttct gccatgaaca cactgaccct
    12421 cgccgatttg cgcccgcaac aaacaggcag cggcaacagc ctgatggcgg tcaaccaaca
    12481 gcttgccatc agcatgggca ttgttgccgg cgcattaatc cttaaaaact ggacatttct
    12541 gataccggct tcttcaggtc tgcattccgc cttccgtatg accctgctca gcatcggcgg
    12601 catcaccctt gcatcatcgc tggttttcaa acggctgcac gtttcagacg gcaccaacct
    12661 gacacggaac acaccgtcct gaagcggtcc acacgcaaaa cttttacccg tttcaacgtt
    12721 tggattatga taccgcactt ccatgcgcgc caaccccaaa acacaggcaa tgccgtctga
    12781 aaccatatcc ctgatgaaaa cacgcagcct aatttccctt ttatgcctcc ttctctgttc
    12841 atgttcttca tggttgcccc cactggaaga acggacggaa agccgtcatt tcaatacttc
    12901 caaacccgtc cgcctggaca acatcctgca aatccggcac acccctcata ccaacgggct
    12961 atccgatatc tatctgttga acgaccccca cgaagccttt gccgcccgcg ccgcccttat
    13021 cgaatctgcc gaacacagcc tcgatttgca atactacatc tggcgcaacg acatttccgg
    13081 caggctgctg ttcaacctcg tgtaccttgc cgcagaacgc ggtgtgcgcg tacgcctgct
    13141 gttggacgac aacaacacgc gcggattgga cgacctcctg ctcgccctcg acagccatcc
    13201 caatatcgaa gtgcgcctgt tcaacccctt cgtcttacga aaatggcgcg cactcggcta
    13261 cctgaccgac ttcccccgcc tcaaccgccg catgcacaac aaatccttta ccgccgacaa
    13321 ccgcgccacc atactcggcg gacgcaatat cggcgacgaa tacttcaaag tcggtgagga
    13381 caccgttttc gccgacctag acatcctcgc caccggcagc gtcgtcggcg aagtatcgca
    13441 cgacttcgac cgctactggg caagccattc cgcccacaac gccacgcgca tcatccgcag
    13501 cggcaacatc ggcaagggtc ttcaagcact cggatacaac gacgaaacgt ccagacacgc
    13561 gctcctgcgc taccgcgaaa ccgtcgaaca gtcgcccctc taccaaaaaa tacaggcagg
    13621 acgcatcgac tggcagagcg tccaaacccg cctcatcagc gacgaccctg caaaaggact
    13681 cgaccgcgac cgccgcaaac cgccgattgc cgggcggctg caagacgcgc tcaaacagcc
    13741 cgaaaaaagc gtctatctgg tttcacccta tttcgtcccc acaaaatccg gcacagacgc
    13801 actggcaaaa ctggtgcagg acggcataga cgttaccgtc ctgaccaact cgctacaggc
    13861 gaccgacgtt gccgccgtcc attccggcta tgtcaaatac cgaaaaccgc tgctcaaagc
    13921 cggcatcaaa ctctacgagc tgcaacccaa ccatgccgtc cctgccacaa aagacaaagg
    13981 cctgaccggc agctccgtaa ccagcctgca tgccaaaacc ttcattgtgg acggcaaacg
    14041 catcttcatc ggctcattca acctcgaccc ccgttccgca cggctcaata ctgaaatggg
    14101 cgttgttatc gaaagcccca aaatcgcaga acagatggag cgcacccttg ccgataccac
    14161 acccgaatac gcctaccgcg ttaccctcga caggcacaac cgcctgcaat ggcacgatcc
    14221 cgccacccga aaaacctacc cgaacgaacc cgaagccaaa ctttggaaac gcatcgccgc
    14281 aaaaatccta tccctgctgc ccatagaaag tttattatag aaatatagtg gattaa
//
LOCUS       DAWFIT010000052        14211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFIT010000052 DAWFIT010000000
VERSION     DAWFIT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.052"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(228..551)
                     /locus_tag="WGQ85_001371"
     CDS             complement(228..551)
                     /locus_tag="WGQ85_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0750784.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WGQ85_001372"
     CDS             complement(632..1288)
                     /locus_tag="WGQ85_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0750785.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1390..1893)
                     /locus_tag="WGQ85_001373"
     CDS             complement(1390..1893)
                     /locus_tag="WGQ85_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0750786.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2077..2850
                     /locus_tag="WGQ85_001374"
     CDS             2077..2850
                     /locus_tag="WGQ85_001374"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0750787.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2857..3207
                     /gene="secG"
                     /locus_tag="WGQ85_001375"
     CDS             2857..3207
                     /gene="secG"
                     /locus_tag="WGQ85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0750788.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3221..3306
                     /locus_tag="WGQ85_001376"
     tRNA            3221..3306
                     /locus_tag="WGQ85_001376"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Leu,seq:gag)
     gene            4107..4475
                     /locus_tag="WGQ85_001377"
     CDS             4107..4475
                     /locus_tag="WGQ85_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0750789.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4472..4783
                     /locus_tag="WGQ85_001378"
     CDS             4472..4783
                     /locus_tag="WGQ85_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0750790.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5120..6090)
                     /locus_tag="WGQ85_001379"
                     /pseudo
     CDS             complement(5120..6090)
                     /locus_tag="WGQ85_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(6113..6226)
                     /locus_tag="WGQ85_001380"
     CDS             complement(6113..6226)
                     /locus_tag="WGQ85_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0750791.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
     gene            complement(6356..7147)
                     /locus_tag="WGQ85_001381"
     CDS             complement(6356..7147)
                     /locus_tag="WGQ85_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0750792.1"
                     /translation="MNLFFDTELGRQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEKKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            complement(7379..10111)
                     /locus_tag="WGQ85_001382"
     CDS             complement(7379..10111)
                     /locus_tag="WGQ85_001382"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0750793.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADTSKADLEASALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            10381..10896
                     /locus_tag="WGQ85_001383"
     CDS             10381..10896
                     /locus_tag="WGQ85_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0750794.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10903..11346
                     /locus_tag="WGQ85_001384"
     CDS             10903..11346
                     /locus_tag="WGQ85_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0750795.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11515..13572
                     /gene="ppk1"
                     /locus_tag="WGQ85_001385"
     CDS             11515..13572
                     /gene="ppk1"
                     /locus_tag="WGQ85_001385"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0750796.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13805..>14211
                     /locus_tag="WGQ85_001386"
     CDS             13805..>14211
                     /locus_tag="WGQ85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750797.1"
                     /translation="MKITHCKLKKKVQKELIRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDKSDFGGRRKGRRGRGAAGKVVVFGILKHNGRV
                     HTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLS"
BASE COUNT         3531 a         3991 c         3494 g         3195 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcagac tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa accaagcaaa atgccgtctg aagccccaaa
      601 cccgctttca gacggcatcg caatccgaac atcaaaactg aaaaccacca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgcccctgcg cgtaatcaaa agcgcgcgtt gaacaggctt gcgtcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacttcagg cacaaggttt accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcaacccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 ccgcatcgat atcgtccgaa attaccctgc ccgccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctccagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaagtcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg ttccaacaat
     1321 agcaggcatt ataaaaccac actcccgccg cgccaacttc ggcacgcgcc gcccgcccct
     1381 ttccctcact caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ctcgcccaaa tacaaagcat ctgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgcgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gtccacatcc ccgacgacca ccgtcccgcc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgtctcg tccgccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacaa cctgcccgaa acaacattcc aaaccgcccc cgcagccgcc
     1981 cgccccaagt tgcgaacccg ccgcatatcc actaaacttc acattgcaac gcgccacacg
     2041 cggcagacag aaaaaacacg acacggagca aaaaagatgt atcaccaaat cggaatgtgg
     2101 gatcaaaaat gggtcatcgg caactggaaa atgaacggcc ggctccagaa caacaattcg
     2161 ctcatgcacc gcttccgcat cctccccacc gccgaacgcg tcctcatcgg actcgccgcc
     2221 ccgaccgttt acctgctgca actgcacaac gccatgcaaa tcgtcttaaa caaccgcatc
     2281 ctcacctgcg cccaagacgt gagccgcttc cccgacaacg gcgcgtacac cggcgaagtg
     2341 tccgccgaaa tgctcgccga caccggcaca gacatcgtcc tcatcggaca ctccgaacgc
     2401 agcctttatt tcggcgaaaa aaacgaaatc caacgccgca aaatggaaaa cgtcctcaac
     2461 gtcggactca tcccgttatt gtgcgtcggc gaaagcctcg aagagcgcga agccggcaaa
     2521 gaacacgaag tcatcgccca tcagctttcc atcctgcaag ggctggatac caaaaacatc
     2581 gccgtcgcct acgaacccgt ctgggcgatc ggcaccggca aagtcgccac cgtcgaacag
     2641 attgccgata tgcacgcatt catctacaaa gaaatcttgt ctttgtgcgg aagcgatgtt
     2701 aaaatccgcg tcctttacgg cggaagtgtg aaagcggaca acgcggccga catcttcgca
     2761 gtaccttatg tggacggcgc actcgtcggc ggcgcgtcat tgtcgtacga ctcctttacc
     2821 gccatcatca gtgccgcaca aaatgcgtag aaaaatatgg aagccttcaa aaccctaatt
     2881 tggattgtta atataatttc cgctttggcc gtcatcgtgt tagtattgct ccaacacggc
     2941 aaaggcgcgg atgccggcgc gactttcgga tcgggaagcg gcagcgcgca aggcgtattc
     3001 ggctctgccg gcaacgctaa cttcctcagc cgctcgaccg ccgttgcagc aacatttttc
     3061 tttgcaacct gcatggctat ggtgtatatt cacacccaca cgacaaaaca cggtttggac
     3121 ttcagcaacg tacaacaaac tcagcaagca cccaaacccg taagcaatac cgaaccttct
     3181 gcccctgttc ctcagcagca gaaataacag tttttcaaat gccgacatgg tgaaattggt
     3241 agacacgcta tcttgagggg gtagtggccg taggctgtgc gagttcaaat ctcgctgtcg
     3301 gcaccaacac acaaaacgct tagaaattct aagcgttttt ttcgtttaat cctgcctgat
     3361 gccatcaacc aatcccaatc aatacaaggt tttcaggctg attgttatcc tgccgccccc
     3421 ccttcctgac cgtacaatcc cgtccaatcc gccctaattg aactaacctg attttttacg
     3481 gcatccgaaa ataccgcgaa aaatacgtga aaataaagct gaacgaccac caaatcagga
     3541 atgccaagcg ggaaatagct tgcggggaat actgacgcac cgtagggaat gagggggaaa
     3601 ccgtccaaga tgcagagcgg ttttttttgg gtttttggaa aaaacctata ctatagtgga
     3661 ttaaatttaa actggtacgg cgttgcctcg ccttgccgta ccggtttaaa tttaattcac
     3721 tataaaataa gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg gtaacgaatc
     3781 ggtaacaact tttggggttt tccggttttt caccgtcttg gcagtgggag cgtagcggaa
     3841 tgaaaagcca aaacgcacgg aaccgcgcct attttgagca ggaatggcgg ttaaaccatt
     3901 tggttatata tgggggcata ggaagacagc gaaacgcgct aaaaacgcaa tttgagccat
     3961 taaaagcgat tgattaaaaa ataatcaggt tagccgccag tgtttcaggc ggcataaacg
     4021 gagaaattgc ggggcataaa aaaggcagct tgccgtgttg tctgtctctg gtataattcc
     4081 aagtatcact aatcaacggc tacacaatgc ggatattcaa aaaccaatgg atagtgaaat
     4141 ttgccaagaa gcacaaaatc aacgattccg agctgctgga agcggtagag cgggcggata
     4201 acgggctgat agacgcagat ttaggcggcg gtgtgattaa gcagcgcata gcaaggcaag
     4261 gacaaggcag aagcggcggc tatcgcagcc tgatattgtt caaacaggca gatagggcat
     4321 ttttcgttta cgcctttgcc aagaacgaca gggaaaacat ttcggataaa gaactggacg
     4381 tttaccgaaa agccgccgca tattatctga aatacacgcg ggcagagctg gcggctttga
     4441 aagaagacgg cattatcacg gagatagaat catgaaatac aaaagcgagg cattagccgc
     4501 cattcatgaa atgatggaag gggcttacaa catcggcgca atcgacaaaa agactatgcg
     4561 cggctttgac aagtcatgcc tgaccgaaat caaaccgttg agcggcggag acatcaaggc
     4621 aatcagggag aaggaggcac tatcgcaagc cgctttcgcc atctatctca acgtgggaaa
     4681 aaatcacgtt tcggcttggg agcggggcgt taaaaagccg agcggcgcgg cgttgaagct
     4741 gctgaccatc gtcaaaaaca agggtatcga agccattgcg tagccgactt ggcaaacggc
     4801 aaaatcagca agttcacaat agacgcgctg ctgaatatgc ctgccaaaac aggcaagacc
     4861 gccgaactga atatcagggc gtagccgcat aaatgccccg accgcatcaa accaagccga
     4921 aacggcggcg gtgcagacgg catagcccga cagcaaggca cggggatttt tttgcgcccg
     4981 ttgcaggggg attggattta agcggcgcgg acttgaaggc aaaacgggcg gggcgcagaa
     5041 ctgtttaaat gccgtctgaa tctcaaacgg attcagacgg cattttggaa caacggctca
     5101 aattctcgat ccccttccct taacgccgac gttttttatt aacgcgcccc ttatttctga
     5161 cactttgctc ataaaccggc ataacggtcg gcaacaaccg ttttagattt tctatacggg
     5221 cattgtttgt cggatgagta gaggtaatag cataaataaa gccgttttgg tcgttttcct
     5281 gattcatttt ttcccaaacc ctgacagcgg ccgccggatg atagcctgcc tgcgccatca
     5341 acatcattcc cccctcatcg gcttcttctt ccaagctgcg gctataaggc aaggtaatac
     5401 cgtacgttcc caaaatatcc atacccaatc cgaccaattc cggattagta tccggttttt
     5461 tgtctaatat aatctgcgtg cctatctgcg ccgccgtatt ggtcaagatt tgctgcccga
     5521 ccttattttt accgtgttca tgcagggcgt gcgtcatttc atgtcccata atggcggcaa
     5581 tttcgtcatc ggtcagcttg agtttgtcga ctatccccgt ataaaacgcc attttcccgc
     5641 cgggcatcgc ccacgcgttc agctcatcgt ttttgaaaac cgtcattttc cagtcaaacc
     5701 tatggccggt attatttgcc gcatcggcat aaggcaacat acgtcgaaat actgcctgca
     5761 ccctacgggc tgttctggat gtggtatcga cattgccggc agacttgttt aactcaaccg
     5821 ttttcatata atcttcggca gccgcagcgt tcattgtggc ggaatcatga ccgtaaacat
     5881 cagcaacgac cgcacaacct cccaatatcg aaatgactgc cgacaggcag actatccgtt
     5941 taaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     6001 ggaaatttca tattaggctt accccttaaa aaattaaatt taaaaaatgc cgtctgaatc
     6061 taaaacggat ttcggacggc atcttaacat tgtttgatgt ttttaaaaag atttacacca
     6121 cgatgctcgc cagtctgccc ggtacggcga tgattttctt ggcaggatta ccttctatga
     6181 atttcactgc gccttcggag gcggccggtt tgtcggaatc acgcataaat tgccaataat
     6241 tccccgactt ttttacggct gctgcctttt gcggcaatat tgcgctgaac ttcaactgtt
     6301 ttcagaatag cagaagaata aatatccctt gtgaattcag tatcatgatt tgaaattaaa
     6361 acgccttgga agtcgggggt aatttgtcga tttttttgta aagtccgcga ccaatgaatt
     6421 cgatcgtatt ttggtcgcgc aaaatttgca actgttggcg gattttgtct ctgatatggt
     6481 tgttttgggg aaattggatg gatagttttt tttcaaattc atacatttgt gacaatgtga
     6541 attcttcggg gagttggtcg atacatttca taacagccaa aagccagcct ttgcgctccg
     6601 cattttggtt gcgtaaaaac aggttggatt gccatttttt cagaacggtt tcgggttcga
     6661 taatgcggga atcgtctatt aagaatattt ttccgctttc aggcaaaggg gcgagattga
     6721 tggaacacat aatgtggttc ggccggtttt taataccttt atttctggga ataatcatat
     6781 ccggcgtgat gaaatgtttg ggtacaagca ccaattgccg tatggagtaa tccgcttttt
     6841 tatatgcaag aaagaaaaag ttggggttgg tatctgaccg gatgcgctcc agcatggtgc
     6901 gatatgcacc gtcaggcacg ctgttgccta tggttttttg atttttactc tttaattcat
     6961 attgctcgtg gcaatttggg caaaagaggt ctgcaacagg tttgttattg gcaaatctct
     7021 gcatcggctt gcttccgcaa caggggcagt agccgttttt ttccagccaa gcctcgctca
     7081 ttacacggat tttgtgggtt gctttatttt gttgccttcc caattcggta tcgaaaaata
     7141 aattcatgtt ttggattttg agattaagtt tgagtggatt aacaaaaatc aggacaaggc
     7201 gacgaagccg cagacagcag ggcaaggcgg ggcaacgccg taccggttta aagttaatcc
     7261 gctatatttg aggtctgtca tgcggacaac tccatctgcc ttaaaaaggc cgtctgaaac
     7321 cctgtttcca agtttcagac ggcctttcct aattacagca gtcaatgccg aatcggtttt
     7381 acaccacgat gttcaccagt ctgcccggta cgacgatgat tttcttggca ggcttgcctt
     7441 ccatgaattt caccgcgcct tcggtggcga gtgcggaggc ttcgaggtcg gctttggagg
     7501 tgtcggcggc gacggtgatt ttgccgcgca gtttgccgtt gacttgaacc atgacttcga
     7561 tttcggattt gaccaaggcg gcttcgtcga ctgtcggcca gcctgcttcc cacagtttcg
     7621 caccgttcaa ttcgctccac agggtttcgc agatgtgcgg cacgatgggc cacagcaggc
     7681 gtacggcggc ttccaatact tcttgggcga cggcgcggcc ttgttcgcca ccggtgtcgg
     7741 ttttgtcgta ttggttgagc agttccatca cggcggcgat ggcggtgttg aactgctgac
     7801 ggcggccgta gtcgtcgctg actttggcga tagtggaatg cagtttgtgg cgcaggtctt
     7861 tgagttcttt agacaaaccg tcttggctgc ctgcgaacgc gttgactgct tcgccttgct
     7921 tcaagtattc gtaaacggta cgccacaggc ggcgcaggaa gcggtgcgcg ccttcgacgc
     7981 cgctgtcgct ccattcgagg gactgttcgg gcggtgcggc gaacatcatg aacaggcggg
     8041 cggtgtctgc gccgtaggcg ttaatcagtt cttgcggatc gacgccgttg tttttggact
     8101 tggacatttt ttccgtgccg ctgatgacga cgggcagtcc gtcgactttc aggacggcgg
     8161 aaacagggcg gcctttgtcg tcaaaagtca gctcgacatc ggcggggttg atccaatctt
     8221 tgccgccttt gtcgttttcg cggtagtagg tttcgcaaac gaccatgcct tgcgtcagca
     8281 ggcgttcaaa cggttcgtca acattgacca aaccttcgtc gcgcatcagt ttggtgaaga
     8341 aacgcgcgta caagaggtgc aaaatcgcgt gttcgatgcc gccgatgtat tggtcgaccg
     8401 cgccccagta tttcgcggcg gcaggatcga ccatgccgtc tgaaaatttt ggcgacatgt
     8461 agcggaagaa gtaccagctt gattccatga aggtgtccat ggtgtcggtt tcgcgttttg
     8521 ccgcgccgcc gcagcatggg caggtggttt cgtaaaactc gggcattttt gccagcggcg
     8581 aacccatgcc gtcgggtacg acgttttcag gcaaaacgac cggcaattgg tcggcaggga
     8641 cgggtacgtc gccgcattgt tcgcaatgga cgatgggaat cgggcagccc cagtagcgtt
     8701 ggcgcgaaat gccccagtcg cgcaggcggt attgggtttt cggttcgcca gctccgatag
     8761 cttgtaattt agcggcgaca gcatcaaatg ctgtctgata attcatgccg tccagttcac
     8821 ctgagttgat taagaccatg aagtcatctt tgactccata ccatgcttgc catttattag
     8881 gcataaacgg caaatctctt gaatcggaac cactaccatc accataacca gcaccacatc
     8941 cggaaccgcc accaaaaccc atagcttctt cttgctctgg cgaaagtccg attgttacgc
     9001 gttgtaagac ctgttttttc ggcaggctgt atttggcggc gaactcgaaa tcgcgttcgt
     9061 cgtgcgccgg aaccgccatt accgcgccgt cgccgtagcc ccacaataca tagttggcaa
     9121 tccacacttc cagcttgtcg ccgttgagcg ggttgacgac gtagcggccg gtcggcacgc
     9181 cttttttctc catcgtcgcc atatcagctt cggcaaccga accggctttg cattcggcaa
     9241 taaatgcctg caattcgggt ttgtcggcgg ctgcggcggt tgccagcgga tgctcggcgg
     9301 caacggcaac ataagtcgcg cccatcagcg tgtcgggacg ggtggtataa acttgcagga
     9361 atttcgcgta atcgccttcc aagccttgtt tgctgtcgtc tgaaacggcg aagcgcacgg
     9421 tcataccgcg agatttaccg atccagttgc gctgcatggt tttgacttgt tccggccagt
     9481 gttccagctt gtccaagtcg ttgagcagtt cttcggcgta atccgtgatt ttgaagtaat
     9541 acatcgggat ttcgcgtttt tcgatcaatg cgccggaacg ccagccgcgt ccgtcgataa
     9601 cttgctcgtt ggcaaggacg gtttggtcga ccgggtccca gtttaccgtg ccgtttttgc
     9661 gatagacgat gcctttttca aacagcttgg taaacagcca ttgttcccag cggtagtatt
     9721 cgggtttgca ggttgcggtt tcgcgcgccc agtcaatcgc aaaacccagg cttttgagct
     9781 gggttttcat gtattcgatg ttatcgtacg tccaagcggc aggggcgacg ttgtttttca
     9841 tcgccgcgtt ttccgccggc atgccgaacg cgtcccaacc cataggctgc atgacgttga
     9901 agccgtttaa aagtttgaag cggctcaata catcgccgat ggtgtagttg cgcacatgcc
     9961 ccatatgcag cttgccgctg ggatagggga acatggagag gcaataatat ttgggtttgg
    10021 aagcgtcttc ggagacgttg aaaatacggg cgtcgtccca ttttttctgc gccgcaggct
    10081 cgatggcggc gggttgatat tgttcttgca tagtcattct gttttcgctt aaaaacgttg
    10141 gaaaaataaa gtcggcatca attataacag gttgccggaa gcggcgaatc ggcagattgc
    10201 cggcaggatg cgtaaattcg cacgcgcatt attccgtatg ccgtacaaat acaccgcgtt
    10261 tattgatacg cacgtttttt atgctaatat tacaaaccaa aatcaaatgt ttaaaactct
    10321 cctgatgcgg ctcttccgaa caaaaggcag acgggcatcg ggtaaaagag gattctgcat
    10381 atgaaaatca aacaaatcgt caaaccgggc ttggcagtat tggcggcggg cgttctgtct
    10441 gcctgcgcaa ccaaaagcaa cgtcaaagcc gacggcacga ccgacaatcc ggttttcccg
    10501 aaaccctatt ccgtaacgct cgacaacaag cgcggcacat tcccgactta tgacgaactg
    10561 gatcagatgc gccccggcct gaccaaagac gacatctaca aaatcctggg ccgcccgcat
    10621 tacgacgaag gtatgtacgg cgtgcgcgaa tgggattacc tgttccactt ccataccccg
    10681 ggcgtaggta tcgaccctga aaacacttcc ggcgtagaag atgttactac ctgccaatac
    10741 aaagtgattt tcgataaaga caaatttgcc cgcagcttct actggaaccc cgtcttcccg
    10801 aaagatgccg cctgtccgcc gcccgcaccc aaagccgagc cgcaagtcat catccgcgaa
    10861 atcgtgccgg caaaaccgaa acgtatccgc caataatccg acatgccgtt ccgcctgttt
    10921 ttagggatat tatgcggcct gtcaatggtt gcccccgtat atgcacaggg gcaaccggat
    10981 gcggtcaacg cttatattca aaagaaaaaa gtcatcgtaa acacatccaa agcggaactc
    11041 tgttttgccg atgacaggca gtgccacccc gtcctcatcg gtgttgccac gcccaagggg
    11101 acgttcgggc tgacgctgaa cagtaccgac aagcctggat acggcggcga agtcatcggt
    11161 ttcaagcagg agggtgattt tcttttcgcc ctgcaccgcg tttggaatca gataccgtcg
    11221 gaaaggcgga acgaacgcat cgcctccccg tccgtgtccg acaggattat gaccaacggc
    11281 tgcatcaacg tcagcgatgc ggtgtacgaa aaactgcgtc attattttgt gttggaagtg
    11341 atttgaaaca gacggatacc gcacgcgccg gtatctgttt tcacattgcc ccgatgcctg
    11401 aaacaggctg tccgccacgt catgccgtct gaaaccggcg cagatgccgc caagccttca
    11461 gacggcattg cctgcccgct ccgaccgaac aacaaccatc tttgggagaa ccttatgccc
    11521 gaacaaaacc gcatcctctg ccgcgaacta agcctgctgg cattcaaccg ccgcgtgttg
    11581 gcacaggcgg aagacaaaaa cgtccccctt ttggaacgcc tgcgcttcct gtgcatcgtg
    11641 tcgtccaatc ttgacgagtt tttcgaagtc cgcatggcgt ggctcaagcg tgaaaacaag
    11701 ctgcaccccc ggcgcaggct ggacaacggc aaaatgccgt ctgaaaccat cgccgacgtt
    11761 accgaagcgg cgcgctccct gatacggcac cagtacgacc tgttcaacaa cgtccttcag
    11821 cccgagttgg cgcgggaaag catccatttt taccgccgcc gcaactggac gggcgcgcag
    11881 aaaaaatgga tcgaagacta tttcgaccgc gaactgctgc cgatcctgac ccccatcgga
    11941 ctcgacccgt cccacccctt cccgcgaccg ctgaacaaat cgctcaactt cgccgtcgaa
    12001 ctcgacggca cggacgcatt cggcaggcct tcggggatgg cgattgtgca ggcaccgcgc
    12061 atcctgccgc gcgtcgttcc cctgccgtcc gaactgtgtg gcggcggaca cggcttcgtc
    12121 ttcctgtcgt ccatcctcca cgcccacgtc ggcaaactct tcccgggcat gaacgtcaaa
    12181 ggctgccacc agttccgcct gacgcgcgac agcgacttga ccgttgacga agaagacctg
    12241 caaaacctcc gcgccgccat ccaaaacgaa ctgcacgacc gcgaatacgg cgacggcgtg
    12301 cggctcgaag tcgccgacac ctgtcccgcc tacatccgcg actttctgct cgcgcaattc
    12361 aaactgaccg ccgccgaact ctatcaggtc aaaggcccgg tcaacctcgt gcgcctcaac
    12421 gccgtccccg acctagtcaa ccgccccgat ttgaaatttc ccacacacac gccgggcaga
    12481 ctgaaagcct tgggcaaaac cgcgtccata ttcgatttgg tgcgccaatc gcccatcctg
    12541 ctgcaccacc cctaccaatc gttcgacccc gttgtcgaaa tgatgcgcga agctgccgcc
    12601 gaccccgacg tgcttgccgt caaaatgacg atttaccgca ccggcacgcg ttccgaactc
    12661 gtccgcgccc tgatgaaggc ggcactcgcc ggcaaacaag taaccgtcgt cgtcgaactg
    12721 atggcgcgtt ttgacgaagc caacaacgtc aactgggcga agcagctcga agaggcgggc
    12781 gcgcacgtcg tgtacggcgt gttcggctac aaagtccacg ccaaaatggc actggtcatc
    12841 cgccgcgaag acggcgtact caagcgttac gcccacctcg gcacgggtaa ctaccaccaa
    12901 ggcacatcgc gcatctacac cgacttcggc atcattaccg ccgacgaaca aatcaccgcc
    12961 gatgtgaaca cattgtttat ggaaatcaca ggcttgggca aacccgggcg gctgaacaaa
    13021 ctctaccaaa gcccgtttac cctgcacaaa atggttatcg accgcatcgc acgcgaaacc
    13081 gaacacgcaa aagccggcaa accggcgcgg attaccgcca agatgaattc gctcatcgaa
    13141 ccgaccgtca tcgaagccct gtatcgggca agcgcggcag gcgtacaaat cgatttgatt
    13201 gtgcgcggta tgtgcacctt gcgcccgggt gtaaaaggct tgtccgaaaa catccgcgtc
    13261 cgctccatca tcggcaggca gctcgaacac gcgcgcgtgt attgcttcca caacaacggc
    13321 gcggacgata cctttatctc cagcgcggat tggatggggc gcaacttctt ccgccgcatc
    13381 gaaaccgccg cgccgattac cgcgcccgaa ctcaaaaaac gcgtcatccg cgaaggtttg
    13441 gaaatggcat tggcagacaa tacgcacgcg tggctgatgc agcccgacgg cggctatatc
    13501 cgcgccgcgc ccgccgaggg cgaatccgaa gccgacctgc aaaacgattt gtggacactg
    13561 ctcggaggct gacccgcacc gccccaatca aaaaccatgc cgtctgaaac ctttccgttt
    13621 cagacggcat agttttacag caatctaaac agggcggacc ggagtcaaaa acacaccttc
    13681 gccattcctg cacaagcact tcccctatac gctcccaacc ccaagccgcc gcattccaga
    13741 cggcattata gtggattaaa ttttaggggc tgtactagat tagcagatat gttaccctgg
    13801 gaatatgaag ataacacact gcaaattaaa gaaaaaagta cagaaagaac tgatccgttt
    13861 ttttgtactc gaagttaccg cccgttctgc cgccgatatt ttgggtatcc atcccaattc
    13921 ggcagcactg ttctaccgta aaatccgcac ggttatcaac catcatttgg ccttggctgc
    13981 cgatgaggtt tttgagggct ctgtcgagcc ggacaaaagc gatttcggcg gacggcgtaa
    14041 aggcagacgt ggtcgcggtg cggcaggaaa agtggttgtc ttcggcattc tgaaacacaa
    14101 cggacgggtc cataccgttg tggtggataa tgccaagtct gaaacgttac tccctgtcat
    14161 caagaagaaa atcatgccgg acagcattgt ttataccgat agtctgagca g
//
LOCUS       DAWFIT010000053        14177 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFIT010000053 DAWFIT010000000
VERSION     DAWFIT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.053"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..82)
                     /locus_tag="WGQ85_001387"
     CDS             complement(<1..82)
                     /locus_tag="WGQ85_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0750798.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHII"
     gene            complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGQ85_001388"
                     /pseudo
     CDS             complement(83..1126)
                     /gene="galE"
                     /locus_tag="WGQ85_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGQ85_001389"
                     /pseudo
     CDS             complement(1119..2121)
                     /gene="dcm"
                     /locus_tag="WGQ85_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2152..4425)
                     /locus_tag="WGQ85_001390"
     CDS             complement(2152..4425)
                     /locus_tag="WGQ85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0750799.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKVSDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4489..5139)
                     /locus_tag="WGQ85_001391"
     CDS             complement(4489..5139)
                     /locus_tag="WGQ85_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0750800.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5136..5933)
                     /locus_tag="WGQ85_001392"
     CDS             complement(5136..5933)
                     /locus_tag="WGQ85_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0750801.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5933..7096)
                     /locus_tag="WGQ85_001393"
     CDS             complement(5933..7096)
                     /locus_tag="WGQ85_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ0750802.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGQ85_001394"
     CDS             complement(7111..8274)
                     /gene="ctrA"
                     /locus_tag="WGQ85_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0750803.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8493..9611
                     /gene="wecB"
                     /locus_tag="WGQ85_001395"
     CDS             8493..9611
                     /gene="wecB"
                     /locus_tag="WGQ85_001395"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ0750804.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            9613..11250
                     /locus_tag="WGQ85_001396"
     CDS             9613..11250
                     /locus_tag="WGQ85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ0750805.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            11323..12066
                     /locus_tag="WGQ85_001397"
     CDS             11323..12066
                     /locus_tag="WGQ85_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0750806.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            12068..12931
                     /locus_tag="WGQ85_001398"
     CDS             12068..12931
                     /locus_tag="WGQ85_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ0750807.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            12976..13995
                     /gene="galE"
                     /locus_tag="WGQ85_001399"
     CDS             12976..13995
                     /gene="galE"
                     /locus_tag="WGQ85_001399"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0750808.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
     gene            14111..>14177
                     /locus_tag="WGQ85_001400"
     CDS             14111..>14177
                     /locus_tag="WGQ85_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ0750809.1"
                     /translation="MRKILVTGGAGFIGSAVVRHII"
BASE COUNT         4184 a         3206 c         2846 g         3941 t
ORIGIN      
        1 gaatgatatg gcggacaacg gcagagccga taaagcccgc gccgccggta acgaggatgg
       61 tttttttgtt tgcggtttgc atttaatccc catatccgtt ggggttgcgg ctgacccaac
      121 gccacgaatc ctccatcatt tgcgcaaggc tgcgtttggt ttcccaacct gtctcttgtt
      181 tggtatggga cgggtcggca aaggaacacg ccaagtctcc ggcgcgtcgg ggttggattt
      241 gatagggaat atttaagccg gatgcggctt caaaggcacg gatgatttcc aaaaccgaat
      301 aggcgcgccc cgaacccaag ttgaacaaat gtacgccggc aacaccgcct ttcgccttca
      361 ttgccgcgat atgcccttct gccaaatcca tcacatgaat atagtcgcgc attcccgtac
      421 cgtccggggt cggataatcg ccgccgaata ccgacagttg cggcagcctg cccgaagcca
      481 cttggcagat atagggcaaa aggttgttgg gaataccgtt gggctgttca ccgatcaagc
      541 cgctttcgtg cgcgccgatc gggttgaaat agcgcaacaa aatcacgctc caacgcggat
      601 cggccttttg gatgtcggtt aacatccgtt ccaccatcgc tttggatgcg ccataaggat
      661 tagcggtatc gcccgggcgc atatcttccg tatagggcat tttacgtatc atggcgcgac
      721 gcattaaagt aaacgccaaa gcgcgtttat actggttatc cagtttccct atatttttac
      781 gttacaaatc aagttgttgg tattcatcgt gaaaatagaa tttttcggca taacgctgac
      841 tcataaaacc ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg
      901 tttcatggtt gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt
      961 tagtaatcac acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa
     1021 atacatctgc aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt
     1081 tctctttatt accgatatag ttaattttcg gatattgctt attcattttc actcattttt
     1141 aaaatacttt cagcaatagc ttttgccaat aaaggcggta cggcgttacc aacctgctgc
     1201 tgctgggcaa ttttgctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct
     1261 aattcacgaa cggttaacgc ccgattctgt tcatagtgaa aaactttgcg catatctcct
     1321 gtaatacaaa cggctggttt tgttgctgtt gtaacggatg tatttacgga tatcacctgt
     1381 tttcggacgt aatggttcag gaatatcgtt acggttacct ccatttttaa caaatgccat
     1441 tttttctaac atttgtgccg aatgattcat agcttcatga tttgcaacgt gtggattgct
     1501 ttcgccagca gccagttttg gaaaatgtcc tattgctgat ccaacagtct gatgggaaat
     1561 ctgcaaaggt tcgggaaagg aaattttgcc tttatccctc ctcccgataa atatcactcg
     1621 gctacgtatc tgaggaacac cgaaatcggc tgcactcagt atcttacatt ccaccgaata
     1681 accgatattc tgaaatgctt gaataatctc aatacgtgtt ttacctgaat tgtgtgtata
     1741 gagtcgcgct acattttcca taacaaaaaa atatggttgg acaattttaa ctattcggac
     1801 aaactcttta aataaatggt tgcgtgggtc atctgtaaat gtccgtccaa tctttcctgc
     1861 catactaaaa ccttgacaag gtggtcctcc aataatcaaa tcaactgctt gtccgttaag
     1921 acaattgatt aaatcttgtt cggttagtgt ggttaaatct ttttgcagta attgatgatg
     1981 ggggaagttg gtacggtaag tctgacaata atcagactcc atttcaacag aaagcaattg
     2041 ttggaatccg gcttgttcaa aacccaagga taggcctcct gctcctgaga aaaggtcaat
     2101 ataggtaagt ggtgtgtgca taagtcaaaa tccataaact cttcgattat tttaccgctt
     2161 cagcttcgca aacgcatccg ccatcgccga attggctggg gcgcggtcgt tgcgttgggg
     2221 tttgcggccg gctgtccgtt cgcggctgcg gttttcagac ggcattttat gttttgcgcc
     2281 gcccggttcg tcatccaagc gcatggtcag cgcgatgcgt ttgcgtgcag catcgacttc
     2341 cagcactttc actttcacca cgtcgccagc tttcaccact tcgcgcgggt cttggacgaa
     2401 cttgttggac agggcggaga tgtgcaccaa gccgtcctga tggacgccga tgtccacgaa
     2461 cgcgccgaag ttggcgacgt tggaaaccac gccttcgagt atcataccga cttgcaagtc
     2521 gctgatttcg tggatacctt cggcaaacga tgccgtctga aactcgccgc gcggatcacg
     2581 gccgggtttt tccagttcgg acaggatgtc caaaatggtc ggcaggccga agcgttcgtc
     2641 ggtgaagtcg gacactttga tttgcttcac gcgctcgcgg ttgccgatga gttcggcggc
     2701 gctaatgcct tgttgcgcca gcattttggc gacgacggga taggcttcgg ggtggacggc
     2761 gctcgcgtcc aacggctctt taccgccgtt aatccgcaaa aagcctgccg cctgctcgaa
     2821 ggttttttcg cccaaacgcg gcactttcag caattttttg cggctgtcga acgcgccgtt
     2881 ttcatcgcgg taggcgacga tgttttgggc aagagtttga ttcaaaccag aaatccgcgc
     2941 caagagcggg gcggaggcgg tattcacgtc cacgccgacg gcgttcacgc agtcttcgac
     3001 caccgcgtcc agcgatttgg cgagccggct ttggttcacg tcgtgctgat actggcccac
     3061 gccgatggat ttggggtcga ttttgaccag ctcggcaagc gggtcttgca gcctgcgggc
     3121 gatggacacc gcgccgcgca gggaaacgtc caagtcgggg aactcgcgcg ctgccagttc
     3181 ggacgcggaa taaatcgacg cgccggcttc ggacacgacg attttatgca gccccatttt
     3241 cgacatttca cgcaccagtt cgcccgcgat tttgtcggtt tcacggctgg cggtgccgtt
     3301 accgatggcg atgagcttca cgccgtgctg tttaatcagg cgcgacagcg ttgccaacat
     3361 attgttttct tgatgcaaat agacgatgac ggtatccagc agcttgccgg tgtcgtccac
     3421 cacggcgcat ttcacgccgt tgcggtagcc ggggtcgaga cccagcgtgg tcagccgtcc
     3481 ggcgggcgca gcaagcagca agtctttgag attgcgggcg aacacggtaa tcgcgtcggt
     3541 gtcggcggct tctttcaaac gattgagagc ttcaagttcc aacgacaaaa agattttcgc
     3601 gcgccaagtc aggcgcacgg tgtcgcgcag ccatttgtgg ccgtctgaaa ctttgaagcg
     3661 gcgggcgatg atttgctcgt attcgctttg ctgcgtaatc ggcgtgtcgt cgggctgata
     3721 tttgagcgcg atgttcaaca cgccttcgtt gcgtccgcgc aaaaccgcta gcgcacgatg
     3781 gctgggcatc gcacgaatag gttcgcggtg gtcaaaataa tcgctgaatt tttcgccttc
     3841 ggtttctttg cctccaacaa cttgcgcgtg gatttcggct tcgttccaca gcttgtcgcg
     3901 cagcgtgccg atgagttccg cgtcttcggc aaactgctcc atcagaatcg cacgcgcgcc
     3961 gtccaacgcg gctttggcat cggggacgtt ttcgttcagg tagccttgtg cggcggcttc
     4021 cacgtcctgc gactgctcgg caagcaacac gtccgccagc ggctgcaaac cgtgttcgcg
     4081 cgcgatttgc gctttggtgc ggcgtttggg tttgtagggc agatacaggt cttccagcgc
     4141 ggttttgtta tcggcggctt cgatttgcgc cctgaggtcg tctgaaagct tgccttgctc
     4201 ttcaatgctt tttaaaacaa cggctttgcg ctcttccaac tcgcgcagat attgcagccg
     4261 ctcggcaagc cggcgcaact gcgtatcgtc caacccgccc gtcgcttcct tgcggtagcg
     4321 ggcgataaac ggcacggtcg cgccgtcgtc caaaagctcg acggcggcgg tgatttgcgc
     4381 ggcagtcgcg gagagttctt gggaaagaat ttgagtaatg ttcatcaata aaattccaac
     4441 ggacaggccg tctgaaattt cagacggcct gatttaaaaa caatcgcttt aaggcagcga
     4501 attataatat tcgtaggctt tgtccatatc ttcaaactgg tacatatgcc ctttttccag
     4561 caccatcgca ttatcgcaat attgcttcat ggcgctgtgg ctgtgcgaca ccaagatgat
     4621 ggaacggtct ttgcgctttt caaacaactc gtacttacat ttatcggcaa aacgcgagtc
     4681 accaactgca atcacttcgt caatcaaata gcaatcaaac tctaccgcca acgacaaagc
     4741 aaatgccaaa cgcgccttca tacctgaaga ataacgcttg accggctcgt aaagatattg
     4801 ccccaactcg gaaaactctt cggtaaagtt cttcacataa tcgatatcga cattgtaaat
     4861 ccggcagatg aaacgcaaat tgtccatacc ggtcagactg ccttgaaacg caccggagaa
     4921 tgccaaaggc caagaaatac tcattgtccg cttgatttca cccgtggtcg gcggctcaac
     4981 gccactgatc aaacggatga gcgtcgattt acctgcaccg ttgcggccga gaataccgat
     5041 tttctcgccc ttctccattt tgaagctaat atcgtgcaag actgtccgcc aaccttggcg
     5101 ggtcagatag cgtttggaaa cgtgttcaac tgaaatcatt gcggctcaac ccctttgctg
     5161 aatctcgcca ccaccgccaa accaagcagc agcaaaacca agttacacaa caaaatatac
     5221 caaggatttt cgtaagtcgt gacgctgtcg ccaaaatatc cggcacggaa catttctgta
     5281 ccatgcacca tcggaatcat cagcacatag tgttgcaact gctgaggcag gttgtgtacg
     5341 aagaaaaaca cgccggacaa aggcatcatc acaaaactga tggtactcca aactttgcca
     5401 aacggttcaa aatgaaacgc caccgaacag atgaccagcc ccaatccaac ggcaaacata
     5461 gccatcaaaa gccaagccaa caacatatag aaaatatctg ccggtacatc aatccaaccg
     5521 atgataacca atgcgaacat aatcacgacc tgcgcaatgg tggcgccggc aatctccaac
     5581 aacatacgcg caaaaatggt atccaatacg cgtacatttc ggtgatacag caggcttgtg
     5641 ttggcagaaa tcgaaccgat agcatgatta gaagcattac gccacatcat catcatcgga
     5701 tagcctgtca gcgtgaatgc cacgatattc aaagctgaca cgttgtgcat acggaaaaac
     5761 ttccacatca acaccataac caaagtcagc agcaaaggtt cgacaaacaa ccacaaaaaa
     5821 ccaatgttat tacgaccgta gcgagtaata atttcacgca ttaagagtgc gccgattacc
     5881 cttttttgga taagcagcga ctccaaaaac gatgttttat gtagctcttt catcagtttt
     5941 tatgctcacg gatacttgca gacagcaaca cgacaatgcc atagacaatc aaaccgatga
     6001 caaacgttgc cacgatgttg tacaaacggt ttggctcata tgccaagtca ggtttgttcg
     6061 gctgggaaat cacttccaaa tacaattgct gacgatctgc ttccgctttt gcactttcca
     6121 aagaagtcat cgccgcagcc aattgttttt cggccaattc gttttccaaa tacacgcgtt
     6181 gatattccgc cgcctgattg gacaacgaac cctcgccacc gccggaaatc gctttcattt
     6241 gttgcgagat ttctttacgc aaactcttct cacgcgcaat caaacctgga atttgcgggt
     6301 tttcaggcgt aacggctttt acctggtcca gctgggtctg aatgacaatc aactcgtcct
     6361 gtaatttgga taccaaaccc atttgcacat cggattgcgc cttcaagtcg aaaataccgt
     6421 tagacacacg gaattttgtc aattggacag aagcctcttt aacttgctct tcagcagaat
     6481 tgaccacttc ctgagcataa cgaatcgtat cctgacgcgc acgttcgttc agctggttaa
     6541 tcaatacctc accctgttta agcaaagcat cattgatctt ttgagattca ccggcattaa
     6601 acgatgtaac gctcaaattg gaaatgcctg agacagagtc aaaatggatg gataccttat
     6661 cacggtagta ttgataaaac gcctcatcct cgccacgcag gccaaaacca ttaaaacggc
     6721 tgaaaatatc gccttctttt tcataaaaat cgcgaatggg cattttctta cgcaacgcat
     6781 ccaaagccga acgcgactgc atatattcct gcacggtata aatatcgtct tgcgcacggg
     6841 caaaacctgt gccctgcaaa atggcaccca ggccattgag agaagattgg cttttaggcg
     6901 agcgcaccac aaagctcgat tgcgacgtaa aacgatcgga agcgaagaag ccgaaataca
     6961 ccaacgaaat taccgtaggg ataatcaccg ttacccaaaa taaagggctt agctttttaa
     7021 tccaactttt ctttttcggc tttttacgct cggctttggt ttcagtggca actgccacag
     7081 gaagttgctc agacatctta attactcaca ttaattagtt aaattattaa tactgttcgc
     7141 gccactggta accggcgaga acacaaacga caagaatttc tgcacttcag ccaacggcgc
     7201 attcgacaca tacaatacat ctttattctt cacaggaaag cgctgcatag aaaatagcga
     7261 atgcgcatca gccatattca cacgatatac cgttggaatc tctgcctcac tgccataacc
     7321 ttgagcaatc catttatcct gacgttctgc cggcaattcc accaatggcg tatagcggaa
     7381 cacaaacaca ccacgcgcat cagaacggcg atcttgcaaa ccgcccatac ggccaatggc
     7441 ttcagaaagc gataagcctc tggctgaaaa accgatttct tgtgttctcc ccaccgcacc
     7501 catagacgta aaggtatagg gattggtaat catggtaacc acatcaccgc gacgcagcaa
     7561 aatattttgt cgcggatttg caactaaatc ttccaaggca acagttcgta ctacattgcc
     7621 acgtgtcagc tgcacattcg tatcctgcac atttgccgtt gaaccaccta ccgcagccac
     7681 cgcatccaac acacgctcac cggctgccgt caacggcata cgcacactat tgcctgcgcg
     7741 aatcaccgat acatttgccg cattattctg caccaagcgc accatcactt gcggctgatt
     7801 ggccattttt ttcaggcggc ctttaataat ttcctgaacc tgaccaggcg ttttaccgac
     7861 caccgaaata tcgccaacaa acggcacaga aaccgtacca cgtgccgtca ccagttgctc
     7921 cggcaacttg gtttgttgcg cactacccga gcccatcgaa gaaaggccac caccaaacaa
     7981 taccgctggc ggcgcttccc aaatcataat atccaataca tcaccaatat ttagcgtacc
     8041 ggtagaagca taaccatcgc caaactgggt aaaagactga ttcacctgag ctttatacaa
     8101 agactgcgct acagcatgat tgacgtcaat caactctact tcaggaattt gagcttcaga
     8161 ctgttgcccc aatgagacaa ctttttttgc acttgggcca gaggagggaa tagcactaca
     8221 tgcacttccc aaaattaaaa aagaaattac aatacaaaac tttaacttaa gcataaaata
     8281 aaaaatctca ttaagtatga ttgtttttaa ataaatttaa aacctaccag agatacaata
     8341 ccactttatt ttgtagaaca caaacgtgta taatatataa cataaacatc atcttcgaaa
     8401 taatattggg gcttaggaag caaaatcatc aaaaaacgtg ataagctcct aatattttta
     8461 acacattact atattacaca taggatattc caatgaaagt cttaaccgtc tttggcactc
     8521 gccctgaagc tattaaaatg gcgcctgtaa ttctagagtt acaaaaacat aacacaatta
     8581 cttcaaaagt ttgcattact gcacagcatc gtgaaatgct agatcaggtt ttgagcctat
     8641 tcgaaatcaa agctgattat gatttaaata tcatgaaacc caaccagagc ctacaagaaa
     8701 tcacaacaaa tatcatctca agccttaccg atgttcttga agatttcaaa cctgactgcg
     8761 tccttgttca cggagacacc acaacaactt ttgcagctag ccttgctgca ttctatcaaa
     8821 aaatacctgt tggccacatt gaagcaggcc tgagaactta taatttatac tctccttggc
     8881 cagaggaagc aaataggcgt ttaacaagcg ttctaagcca gtggcatttt gcacctactg
     8941 aagattctaa aaataactta ctatctgaat caataccttc tgacaaagtt attgttactg
     9001 gaaatactgt catagatgca ctaatggtat ctctagaaaa actaaaaata actacaatta
     9061 aaaaacaaat ggaacaagct tttccattta ttcaggacaa ctctaaagta attttaatta
     9121 ccgctcatag aagagaaaat catggggaag gtattaaaaa tattggactt tctatcttag
     9181 aattagctaa aaaataccca acattctctt ttgtgattcc gctccattta aatcctaacg
     9241 ttagaaaacc aattcaagat ttattatcct ctgtgcacaa tgttcatctt attgagccac
     9301 aagaatactt accattcgta tatttaatgt ctaaaagcca tataatatta agtgattcag
     9361 gcggcataca agaagaagct ccatccctag gaaaaccagt tcttgtatta agagatacta
     9421 cagaacgtcc tgaagctgta gctgcaggaa ctgtaaaatt agtaggttct gaaactcaaa
     9481 atattattga gagctttaca caactaattg aataccctga atattatgaa aaaatggcta
     9541 atattgaaaa cccttacggg ataggtaatg cctcaaaaat cattgtagaa actttattaa
     9601 agaatagata aaatgtttat acttaataac agaaaatggc gtaaacttaa aagagaccct
     9661 agcgctttct ttcgagatag taaatttaac tttttaagat atttttctgc taaaaaattt
     9721 gcaaagaatt ttaaaaattc atcacatatc cataaaacta atataagtaa agctcaatca
     9781 aatatttctt caaccttaaa acaaaatcgg aaacaagata tgttaattcc tattaatttt
     9841 tttaattttg aatatatagt taaaaaactt aacaatcaaa acgcaatagg tgtatatatt
     9901 cttccttcta atcttactct taagcctgca ttatgtattc tagaatcaca taaagaagac
     9961 tttttaaata aatttcttct tactatttcc tctgaaaatt taaagcttca atacaaattt
    10021 aatggacaaa taaaaaatcc taagtccgta aatgaaattt ggacagattt atttagcatt
    10081 gctcatgttg acatgaaact cagcacagat agaactttaa gttcatctat atctcaattt
    10141 tggttcagat tagagttctg taaagaagat aaggatttta tcttatttcc tacagctaac
    10201 agatattcta gaaaactttg gaagcactct attaaaaata atcaattatt taaagaaggc
    10261 atacgaaact attcagaaat atcttcatta ccctatgaag aagatcataa ttttgatatt
    10321 gatttagtat ttacttgggt caactcagaa gataagaatt ggcaagagtt atataaaaaa
    10381 tataagcccg actttaatag cgatgcaacc agtacatcga gattccttag tagagatgaa
    10441 ttaaaattcg cattacgctc ttgggaaatg aatggatcct tcattcgaaa aatttttatt
    10501 gtctctaatt gtgctccccc agcatggcta gatttaaata accctaaaat tcaatgggta
    10561 tatcacgaag aaattatgcc acaaagtgcc cttcctactt ttagctcaca tgctattgaa
    10621 accagcttgc accatatacc aggaattagt aactatttta tttacagcaa tgacgacttc
    10681 ctattaacta aaccattgaa taaagacaat ttcttctatt cgaatggtat tgcaaagtta
    10741 agattagaag catggggaaa tgttaatggt gaatgtactg aaggagaacc tgactactta
    10801 aatggtgctc gcaatgcgaa cactctctta gaaaaggaat ttaaaaaatt tactactaaa
    10861 ctacatactc actcccctca atccatgaga actgatattt tatttgagat ggaaaaaaaa
    10921 tatccagaag agtttaatag aacactacat aataaattcc gatctttaga tgatattgca
    10981 gtaacgggct atctctatca tcattatgcc ctactctctg gacgagcact acaaagttct
    11041 gacaagacgg aacttgtaca gcaaaatcat gatttcaaaa agaaactaaa taatgtagtg
    11101 accttaacta aagaaaggaa ttttgacaaa cttcctttga gcgtatgtat caacgatggt
    11161 gctgatagtc acttgaatga agaatggaat gttcaagtta ttaagttctt agaaactctt
    11221 ttcccattac catcatcatt tgagaaataa gttaaattat gaagaacctt tgagtgcaat
    11281 tcgaaggttc ttcattcata ttattcatat tttggagaaa ttatgttatc taatttaaaa
    11341 acaggaaata atatcttagg attacctgaa tttgagttga atggctgccg attcttatat
    11401 aaaaaaggta tagaaaaaac aattattact ttttcagcat ttcctcctaa agatattgct
    11461 caaaaatata attatataaa agatttttta agttctaatt atactttttt agcattctta
    11521 gataccaaat atccagaaga tgatgctaga ggcacttatt acattactaa tgagttagat
    11581 aatggatatt tacaaaccat acattgtatt attcaattat tatcgaatac aaatcaagaa
    11641 gatacctacc ttttgggttc aagtaaaggt ggcgttggcg cacttctact cggtcttaca
    11701 tataattatc ctaatataat tattaatgct cctcaagcca aattagcaga ttatatcaaa
    11761 acacgctcga aaaccattct ttcatatatg cttggaacct ctaaaagatt tcaagatatt
    11821 aattacgatt atatcaatga cttcttacta tctaaaatta agacttgcga ctcctcactt
    11881 aaatggaata ttcatataac ttgcggaaaa gatgattcat atcatttaaa tgaattagaa
    11941 attctaaaaa atgaatttaa tataaaagct attacgatta aaaccaaact aatttctggc
    12001 gggcatgata atgaagcaat tgcccactat agagaatact ttaaaaccat aatccaaaat
    12061 atataaaatg cgtaagatta cttttattat ccctataaaa cagtctttaa taaaacctga
    12121 ttgctttata cgcctctttt ttaatttatt tttgctaaaa aaattctcaa gtaaatacgg
    12181 attttctata ttagttgcag acaacagtaa cttcctttgg aaaaatatta ttaaattaat
    12241 tacaaaattt tacaaatgta attatattag tattaaatct cataatactt tttatacgcc
    12301 tgctaaaatt aaaaatgcag ctgccatcta tagttttaat accttgaatt caaattacat
    12361 tttattctta gatgttgacg ttttattatc ggaaaatttt attcaacatt taataaaaaa
    12421 aacaaaaacc aatatcgcct ttgattggta ccctgtttca ttcttaaaca aacaatttgg
    12481 gattataaat tttatattat tctcatataa aggtaatcta aatatagaag aatcattcat
    12541 tatacaaaca gggtttgtaa ctggcttaca attatttaat tctgattttt tctacaaaac
    12601 agctggatac aatgaaagct ttcttggcta tggctgtgaa gatattgaaa tgattcacag
    12661 agcaacatta ttattaaata ttagacctgc ctttaatgaa aatcatcaat attttacaga
    12721 tgatagagga tatatgcctt ctaaattaac cggatttcga aattattttt attatttgaa
    12781 aagagatgaa ttttcaaact tacagataac tcctaaacat ttctggcata agcgaaaaaa
    12841 taaatcaaaa tatctaaaaa atagatatca aaatgatgta aaaatgattc agattatgaa
    12901 agattttgat cgaaaatttc taaaaaatta acgagctgtc ttgcccatat gaatcctgat
    12961 tactttaatt taattatgaa aaatattctc gttaccggcg gcaccggttt tatcggctcg
    13021 cacaccgttg tttctttgct gaaaagcggc catcaagtcg tgattttgga taacctatgc
    13081 aattccagca tcaatatcct gccacgcttg aaaacgatta ccggccaaga gattccgttt
    13141 tatcaaggcg atatccgcga tcgtgagatt ttgcgccgta tttttgcgga aaaccgcatt
    13201 gattcggtga ttcattttgc cggcttgaaa gcggtgggtg aaagtgtggc cgagccgatg
    13261 aaatattatg ataataatgt ttccggcagc ttggtgttgg cggaagaaat ggcgcgtgcg
    13321 ggcgtgttta gcattgtgtt cagttcttcg gcgacggttt atggcgatcc gggcaaagtg
    13381 ccttataccg aggatatgcc actgggcgat accaccagcc cttacggcac atcgaaatca
    13441 atggttgagc gcattctcac tgatattcaa aaagccgatc cgcgctggag cgtgattttg
    13501 ttgcgttatt tcaatccgat tggcgcgcac gaaagtggtt tgattggcga gcagccaaac
    13561 ggcatcccga ataatttgtt gccttatatc tgccaagtgg cggcaggcaa actgccgcaa
    13621 ttggcggtat ttggcgatga ctaccctacc cccgacggca cggggatgcg tgactatatt
    13681 catgtgatgg atttggcaga aggccatgtc gcggctatgc aggcaaaaag taatgtagca
    13741 ggcacgcatt tgctgaactt aggctccggc cgcgcttctt cggtgttgga aatcatccgc
    13801 gcatttgaag cagcttcggg tttgacgatt ccgtatgaag tcaaaccgcg ccgtgccggt
    13861 gatttggcgt gcttctatgc cgacccttcc tatacaaagg cgcaaatcgg ctggcaaacc
    13921 cagcgtgatt taacccaaat gatggaagac tcatggcgct gggtgagtaa taatccgaat
    13981 ggctacgacg attaagttga cctgatacag gccgtctgaa agagatgttt tcagacggcc
    14041 tctttatctg aaaaacacac attctgtctg ctataatctg tttatatttt ttggctatcc
    14101 tctgaaattt atgagaaaaa tccttgttac cggcggtgcg ggctttatcg gctctgccgt
    14161 tgtccgtcat attatcc
//
LOCUS       DAWFIT010000054        13035 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFIT010000054 DAWFIT010000000
VERSION     DAWFIT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.054"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(963..1631)
                     /locus_tag="WGQ85_001401"
     CDS             complement(963..1631)
                     /locus_tag="WGQ85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0750810.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGQ85_001402"
     CDS             complement(1628..2296)
                     /gene="hda"
                     /locus_tag="WGQ85_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0750811.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2401..2979)
                     /locus_tag="WGQ85_001403"
     CDS             complement(2401..2979)
                     /locus_tag="WGQ85_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0750812.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            complement(3201..3413)
                     /locus_tag="WGQ85_001404"
     CDS             complement(3201..3413)
                     /locus_tag="WGQ85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750813.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(3580..4293)
                     /locus_tag="WGQ85_001405"
     CDS             complement(3580..4293)
                     /locus_tag="WGQ85_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0750814.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFAVESLTEL
                     SNDDLQKTYAAVSTKKRKK"
     gene            4526..4777
                     /locus_tag="WGQ85_001406"
     CDS             4526..4777
                     /locus_tag="WGQ85_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750815.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4779..6756
                     /locus_tag="WGQ85_001407"
                     /pseudo
     CDS             4779..6756
                     /locus_tag="WGQ85_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6823..6900)
                     /locus_tag="WGQ85_001408"
                     /pseudo
     CDS             complement(6823..6900)
                     /locus_tag="WGQ85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6971..7885
                     /locus_tag="WGQ85_001409"
     CDS             6971..7885
                     /locus_tag="WGQ85_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0750816.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASVSMYLNGT
                     YADKGGNYETIETKIEAFLEMQESKARREELVLGFVSTKTTRRIAEVMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            8210..8449
                     /locus_tag="WGQ85_001410"
     CDS             8210..8449
                     /locus_tag="WGQ85_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750817.1"
                     /translation="MSHLFCERKTKWIGLAFWLLLFGVVLVGTMLHSRSKPKVSAAKL
                     EMSRRELLADLEAKARVEQYEAMSTEEKMKGIVYE"
     gene            8552..8725
                     /locus_tag="WGQ85_001411"
     CDS             8552..8725
                     /locus_tag="WGQ85_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750818.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            8718..8885
                     /locus_tag="WGQ85_001412"
     CDS             8718..8885
                     /locus_tag="WGQ85_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750819.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8949..9176
                     /locus_tag="WGQ85_001413"
     CDS             8949..9176
                     /locus_tag="WGQ85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750820.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            9191..9709
                     /locus_tag="WGQ85_001414"
     CDS             9191..9709
                     /locus_tag="WGQ85_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0750821.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(9869..10084)
                     /locus_tag="WGQ85_001415"
     CDS             complement(9869..10084)
                     /locus_tag="WGQ85_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750822.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(10088..10686)
                     /locus_tag="WGQ85_001416"
                     /pseudo
     CDS             complement(10088..10686)
                     /locus_tag="WGQ85_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(10818..11018)
                     /locus_tag="WGQ85_001417"
     CDS             complement(10818..11018)
                     /locus_tag="WGQ85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750823.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(11219..11404)
                     /locus_tag="WGQ85_001418"
     CDS             complement(11219..11404)
                     /locus_tag="WGQ85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750824.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(11677..11844)
                     /locus_tag="WGQ85_001419"
     CDS             complement(11677..11844)
                     /locus_tag="WGQ85_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750825.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            12081..12323
                     /locus_tag="WGQ85_001420"
     CDS             12081..12323
                     /locus_tag="WGQ85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750826.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            12367..12558
                     /locus_tag="WGQ85_001421"
     CDS             12367..12558
                     /locus_tag="WGQ85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750827.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            12555..12971
                     /locus_tag="WGQ85_001422"
     CDS             12555..12971
                     /locus_tag="WGQ85_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750828.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         3475 a         2641 c         3438 g         3481 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 aggctccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatt actttcctag agtaagggta
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtaaat gcagagtggg tggaaacact cactttatgg tttgctacgc tctgttcaat
      661 tagcaacccg ataacccaat atagataata gggtaattaa tctaatctaa tttgtcagca
      721 tccgttaatt tattgcaaaa taaagtattg aattatgtcg ggtgcaaatg acgaaatata
      781 agtttccgtg cggacggatc aagattccca ctttcgtggg aatgacggtg gaaagattgt
      841 tgtttttccc gatgaattcc tgtgtttttt gtttttccgg ataaattcct gtggctttga
      901 gttttttgga tttcagcctc aatgccgtct gaacgccgaa tcgggcttca gacggcattg
      961 cgtcatttga aattcaaaac cggccagcct ttttctttgg cttctttttc cagctcggca
     1021 tcggggttga cggcgacggg ttcgtcgacg aggcgcagca gcggcaggtc gtttttggag
     1081 tcgctgtaaa aataggtttt gccgtagctt tcgagcgttt cgccgcgttc ggcgagccat
     1141 tggttcaggc gggtgatttt gccttctttg aggctgggcg tgccgatgta attgccggtg
     1201 tagcggccgt cagaaccggt ttcgagttgt gtgccgatga tgttggtgat gccgaaaagg
     1261 tggcagacgg gggtgatgat gaactcgttg gttgaggaaa tcacaagggt ttcgtcgcct
     1321 gccatttggt ggctctgcac cagcatacgc tgcataggcg agatgtgggg gatgatgtat
     1381 tccgccataa attcgcggtg aaactctgcc agctcttctt tgctgtaacg agcgagcggg
     1441 gcaaggtgga atttgaggaa tgcgtcgatg tcgaggcaac cgttttggta gtcgcggtag
     1501 aatttttcgt tttgcgcttc ggtttcggca gcgtcaacca agcctttttt gatgaggtat
     1561 tgcggccagg cgtggtcgga atcggtgttg atgagggtgt tgtcgaggtc gaagatggcg
     1621 aggtttttca ttgggtttcc tgttgtttca aaagctggcg caaaagcggc agggtgatgc
     1681 gtttgcccat tgtgacggcg tagttgtcca gcgtgtcgag catcatcatc aggctgtcca
     1741 tatcgcgccg ccagtgtttg agcaggtatt cgaaaatttc ggaatcgacg gttacttggc
     1801 gtgccgccgc catactggcg agcgcgtcga ttttttcttg gtcggttaag ggcttgactt
     1861 cgtaaacgag gcagtacgcc atacgcgtcc gcaaatcttc gcggatgacg agctgctggg
     1921 gcgtgtattc cgaaccgagc agcaaaaagc ctttgccgct gttgcggaag cggttgaaga
     1981 tggaaaaaag cagggcttgt tcttcgttgc ccagtttttc gacttgatcg acggcgaggt
     2041 attccgcctc gaacgcggca tcggtcagcg gcatggaggc ggcatcgata taggcggcgt
     2101 ttttgccggc ttcgagcgcc tgtgcgaccc acgcctgcaa aagatggctt ttgcccgcac
     2161 cttcttcacc ccagacatag ataaactgtc cgtgtttgtg tcggaggaca tagaccagtt
     2221 ccgcgttttc cgtgccgagg aatttgtcga aactcggata gtcgtgtgcg gcaaagtcga
     2281 aaataagctg gttcacggtt cggcattccg aggggtggta aacgggttta ttgtacgttg
     2341 ttttcgcgtg ctttatcaat ttgaacgatg ccgtctgaaa acggcttcag acggcatcgt
     2401 tcaaccgcag gcaacgttgc cgacatcgag gcgcatattg tggaacgcgt tgagcgtgct
     2461 tcggtgtccg atgctgatga tgatgctgtc gggcagtttt tgctttaagg cgcggtagag
     2521 cgcggcttcg gccggttcgt ccaaggcggc ggtggcttcg tcgagcagga cgattttggg
     2581 cttggaaagc agggcgcgga cgaaggcgac gcgttgcagt tcgcccaggg agagtttgtg
     2641 ctgccagtcg tctgttttgt ccaatttgtc aatcagataa cccaagcggc aggtgttcat
     2701 ggcttcgatt agctccggat gctgtttgtc gatgtcggga taacaaaccg cgtcgcgcag
     2761 gctgccctgt gccgtgtacg ggcgttgcgg caggaagagg atgtcttgat gcggcggacg
     2821 gctgactttg ccgctgctgc cgaacggcca aagcccagcc agcgcgcgca gcaacgaagt
     2881 tttgccgcaa ccgctcgggc cgcgtatcag cagggaatcg ccgcttttga gtttgatgtt
     2941 gatgccgctc aacaggattt cgccgttgtg gcggaacagg gcgacgtttt cctgtggggg
     3001 actgttagtt tttgcacaag gaacaaatag agtaaaaaaa cgctgaaatc ttcggaagac
     3061 gtggatttcg gcgttttttt gtatccggaa aagttacgcc agctttttca caaaaccgcg
     3121 ccggaatgcg cggttttctg tttaaagctg acgagattag ggaattttta aaactgtttt
     3181 aagaggtttt taaaatggat ttaatcaata ctccggccat accactcaac acggcctatg
     3241 atggcgatgt cgtcttgggt attactcaaa tctatttcaa acggtgcgta acgtggattt
     3301 tcagacgtta caagcagttt gcccggtata cgttgcacac gtttgacaaa gaggtcattg
     3361 cctatacgca agacatatag gccgtcgcgc ggctcggttt cggcgtggtt gattagaatg
     3421 tttataaaag gctttaacta gaaactgaac atggaacttg tataatagtt ccatgtttga
     3481 aaaaatcttt taatatcata catctaaaaa aacaaaacat ctgaagacca taagctaaac
     3541 atgaaacttg aatttaaagg cggagtttca tgtttaagcc tattttttcc gttttttagt
     3601 cgatactgct gcatatgttt tttgcaaatc gtcattactt aattcagtca ggctttctac
     3661 tgcgaaattt ttttcgaggt aacttttcaa ccaatcttca agctcaagtt gttttacatt
     3721 taatttgata taagcgtatt tcttttttcg ccagtcggga tcgttgttct ttgatgttgc
     3781 tgtattgctc aacctaccca gccattttct gagataagtt tctgctttgt cgtaatcgct
     3841 caatgcgatt agtttgtaac ttggaacttt acagtgagca ttcagactag cccaaattgc
     3901 acggattgat tttggcgacc gtttggcaat ctcttctgct gcggcaactt gtttaacaag
     3961 ttgttgcagc tttgaagctt gctcaagcgt tatatgttcc ttgccgggtt ttgaatcgac
     4021 acgggttctg atgacgtgat tttgggtatt aatttgatga acaaccgaac cagatgaggc
     4081 gataacagcg ttactgacgt tgccgttaat ctgattctca acgtttgatt tgccaatagc
     4141 gtcgtccaat tttttattaa ggtcgtctga aacagcattt aaatttgccg aatgaatgcc
     4201 tgtaaagaca aattgaatat ccacccctaa ttggacagca gcaagtaaaa agtccgcagg
     4261 aatgttgacc tgcccgcttt catacagtct caaactttct gcactgcatc cagtttgctc
     4321 agcaaaattc tttcgagagt atccaagtcg gcttctctct ttaactaaat tcaatgctaa
     4381 atcaatgcgt tccataaaac accgcaaaat agtacccaat atatttggta taccaaatat
     4441 attgggtata ttatccaggt aacagttaag gcaggcatta aagcctgtaa ggtaagtaag
     4501 taatggattc tatcatggag gcattatgca agcgaacgaa atctataaat ccttagaaga
     4561 aaaaaatata tcggcaagaa tgttggcttc ggctttaggg gtaactaacc aatctgtttc
     4621 tgaggtcata agaaatggac gaggaagcaa aagaattgcg gaggcaatcg cgaaagtgat
     4681 tcaaaaagat ttagttgaag tttttccgca ttacaagaaa acagattgtc gagacaaaaa
     4741 gatagctgaa ttgaaagaga tgctcggagt gggttgaaat ggaatatatg gatctcaaaa
     4801 gcctgcttaa ttttgggttg ccaaatatgc caagaacagt aatagggctg agaaaaaaaa
     4861 gcgcaaaggg atggttggca aacgagaagg cgtcaaggta agggtggtgg tgttgaatat
     4921 gccctaccta ttgaaatccg agcagccatc atgaaacggc agtcggacga gctggcggaa
     4981 aagatgccga aaatgttgcc ccaagtcaga ccggggacgg cgatgtcggc tcaggcactg
     5041 gctgaagcgg ccaagctgtt gaacgagaaa caacggtcgg tggcggatgc gcgatgtgcg
     5101 gtggtggcgg cggtattggg gattaaatac caatacggtt gctctgcaaa ggctgcggtg
     5161 gctcagtttt tgggcttgct ggcagaaggt aaattggacg cggtcacgct tgggaacttg
     5221 gaaaaggcca atgaccgcag ccggtcggca aaggttggcg aacgtacttt agacggctgg
     5281 atttctgctt atttgaaagc ggaaaacgcg acggagcggt tggttgcttt ggctccgaag
     5341 acgacgaagg cggtcaagcc gattgagagc tacggttggt tgccgatgtt tatgcagttt
     5401 cacaacatcc cttcggctcc gaaactggcg cacagctacc gccggtttgt gcagtgggcg
     5461 gaagcggaaa atatgccggt caatgatgtg cctaacttga gtatggtgcg gcgcgtttgg
     5521 gacaagctgc cgatgattat gcaggagcgt ggcaggaaaa cgggggcggc ttataaatcg
     5581 ctgctgcctt atgtgaaacg tgattggggg gctttgaagc cgaacgatgt ttggatcggc
     5641 gacggccaca gctttaaggc gaaggtggcg catccggtac acggcaggcc atttaagccg
     5701 gaagtgacgg tgattattga tggttgtacg cggtttgtgg tcggtttttc ggtctctctt
     5761 gctgaaagtt gtgtggcggt atcggatgct ctgcgtatcg gggtcaagca ttttggtttg
     5821 ccgattatct attactcgga taacggcggc ggccaaaccg gcaagacgat agaccatgaa
     5881 atcacgggta ttacgtcccg actgggtatc cgccatgaaa cgggtatcgc gggcaacccg
     5941 caagggcgcg gcatcatcga gcgatggtgg aaagacaatc tgattgagat ggcgcgccag
     6001 tatgagacgt ttgcgggcgc ggggatggac agcagcacga agaacctgat gtaccgcaag
     6061 atggaaagtg cttttaatgc tttggaaaaa ggcaaggatt tgacggagga acaacagaaa
     6121 tatttgaaaa aactgccgag ctggtcgcgt ttcattgcgg atgtggtcaa atgtatagac
     6181 gaatacaaca accgcccgca cggcgagctg ccccgacatc ctgacggcgg gcattatacg
     6241 ccgaaggctt atcgggaaat gaggctggaa caggacggta tcgcgccgga tatgttgtcg
     6301 gcgcaagagc tggcgacgat gtttatgccg caggaagtgc gaaaggtaca gcgcggttgg
     6361 ctggatttgt tcaacaactc ttatttctca accgagctgg cggagtatca caaggacgag
     6421 gtacgggtca gctacgattt gagcgatgcg tcggcggtca atgtgtttga tatggacggc
     6481 aagtttatca ctaaggcgca ggccaacggc aatacccgcg aggctttccc gacggctcgt
     6541 atcgaccaac tggcagaaaa acgccgaaaa ggcaaaatca agcgggcgga aaatgcaatc
     6601 aagcttgcaa acgcggaagt caatccggca ctggaacagg cggcagtttg ggacgagctg
     6661 ggacatttgg gcggaaacgt catcgaggcg gagtatgcgg tattgccgaa aacgggtacg
     6721 gacgacggga ttttcttgtt tgaggcggat atgtaaagga aaacatgatg gaaaaacaga
     6781 aaaatgcggc gttttcggcc gagcttgttg aaaaattgaa actcaagcga gcccttgggc
     6841 ggattcaacg atttcaagca aagattcaag gtgttcccgc tgaacggaat caggctcaaa
     6901 tttctaaatc gggaaactct ttcatttcag acggccttta aaggttgttt aaaactcaag
     6961 gatattaaaa atgaaacaaa ttaatcaagc attgcaacaa aaactggttg aatttaaaga
     7021 aaaatcaggt atgaaccaga cccaactggc acgcggtatc gatacttcgc cggcatccgt
     7081 cagtatgtat ctgaacggca cttatgccga taaaggcggc aattatgaaa ccatcgagac
     7141 gaaaatcgag gcgtttttgg agatgcagga aagtaaggcg cgacgtgaag agctggtgtt
     7201 gggttttgta tcgactaaga cgacccgccg aatcgcggaa gtgatgcgcg atgcgcacga
     7261 aggcggcgaa atagtggtga tctacggtca ggccggtttg ggcaagactc aggcggtcaa
     7321 aaactactgc gagaaaaacc ctgcggccat cttgattgag gctaatccga gctttacggc
     7381 tttggtcttg atgcgcaagt tggcaacggc ggcgaaggta tcggcgatgg gcagcctgaa
     7441 tgatttgttt gagtctgtat ctgaccgcct gcgcgattca ggccgtctga ttgtagttga
     7501 tgaagcggaa aacctgccgt tacgcgccct tgaaattgta cgccgtctgc acgatgagac
     7561 tggctgcggc ttggtgttga gcggtatgcc ccgactggtt gcgaatttgc gcggtaagca
     7621 tggcgagctg gtgcagcttt acagccgcgt gtcggttgcg ctgaatttgg gcgaatcttt
     7681 gccggatgac gaactctttg agattgcgaa agcggctttg cctgatgcgg acgaagaaac
     7741 gctcttggaa ctggttaaac atagtaacgg caatacgcga cggatgagca agttgatgcg
     7801 cggtgcggta cgcacggcaa acaaaaacgg catcaagatg caggccggta tcgttaagaa
     7861 atacagctcc ctgattatcc gataagaaag gccgtctgaa atgagacaag aatatgcggt
     7921 acacgccggg gtctatgagg acacttggca cgattatgaa acccataaac ggaggaagat
     7981 ttggcgcggc aagcgcaaag agggagttgc cctctccccc cagccctctc ccacggggag
     8041 agggagcgaa gggggcgcag acggcagggt tcggagttgc cctctccccc agccctctcc
     8101 cacagggaga gggagcgaag gcggtgcgcc ggttagggcg gattgggtac gcaataattt
     8161 ttttgcctta agaaaatatt aattaattga tttataagga aatatgaaaa tgtctcattt
     8221 gttttgcgaa cgaaaaacca agtggatcgg tttggctttt tggttgttgt tgtttggggt
     8281 ggttttggtg ggcacgatgc tgcacagccg ctctaagccc aaggtgtcgg cggcaaagtt
     8341 ggaaatgtcg cgccgcgagc ttctggcgga tttggaggca aaagcccggg tcgagcaata
     8401 cgaggcgatg agtacggaag aaaaaatgaa agggattgtt tatgagtgat aagccattga
     8461 gccatgcggc gaaaaaagag gctttggatc gggcggtcaa ggaaatccgc gcgaaatatg
     8521 gcgataaggc gattgtgaaa ggatgtgtga aatgagtttc ggacgacgta atacggattg
     8581 gcaggcttgg ggacaacacc gcaggcgtgc gacggcgcga atggcgcaaa aaagccggga
     8641 gcgcgaaatc gaggaatatc aggcgcagtt tgaagataag gacggcaaag gtcgtctgaa
     8701 aacgggagga aacaaagatg aatgaaaaag atttgatcga atggctcgaa gaccgtggcg
     8761 agctgatggt catgaagaag gacggcgaag gctttgtgat tgcagcgcgt gcgccggacg
     8821 ggatgtggaa gactgccgag gcggaaactt tggctcaggc gataacttta tgggaggaag
     8881 tgtgatgttt aagtttttga gaaaccgcgc gaaagttgaa gcaattttaa acaagaaagg
     8941 aaagtgaaat gaaagtgtat gtttttaaaa ttagtaatga aaacggtaag ttaaagatag
     9001 aactccccga aatcccaatg ggtaagcaaa ttgacgaagt tgatttgatt gctgggctaa
     9061 ccacggaatt tattgcaagt atgttacgtg atgcccaaaa agatcgtcgc aaattcgtaa
     9121 tcgacgcatc aaatcaattg gctgcaatcc agacatatca aaaaatcttc aattaaggaa
     9181 ggaagagaaa atggctaaaa cccgaatcaa acagcccgcc atcgaagcgg cacaagacaa
     9241 aacggaagtt actgcattta tccgccaaat cggcgacttg cagcgcgaag tcaaacgcct
     9301 ggaaaccgaa gccggagaca aaaaagcggc tatcgaagaa gaatatgccg ccaaagccgc
     9361 gccgatgtgt gccgaaatca tgagcctgac cgaacgtgtg gccgcctact gcgaagcgca
     9421 taaggacgag ctgacggaaa atggtaaaac caaaaccgtg gactttacta ccggcctgat
     9481 taaatggcgc attcgtccgc catccgtcaa ggtaacgggc gtggccgccg tcttggcgtg
     9541 gctctcggaa aaatccgcct ttgccgagtt tgtccgcact aaaaaggaaa tcgacaaaga
     9601 cgccatcctg aatcaaaaag agcgtttttc agacggccaa gtgccgggga ttaagattgt
     9661 gagcggcgtg gaggattttg tgattgagcc gagcgaacaa attctataaa atcaacagcc
     9721 tgatttcaat tcagtgtgtt gaaacgtgat tgagcctacg gagcaggagc ttgcctagcc
     9781 ggttcaaata ggctttaacc catgattaaa ggccgtctga aatggggttt aaaacctgtt
     9841 tcagacggcc ttttttcata ccctagattc aggctgcttt ctcttcctgc tcgtacacgg
     9901 cgttgtagaa agcggcggac agcttgtcgg ctttctcgga ggcggtttca atcacggcag
     9961 acaaaccgtc ttcgattcct tccatgtcca tatccaaaac tttcagatgg ttgagcgtga
    10021 acaccagcag attcagggct ttgaggctgt cttggtcgaa agtcagagta taggtggtat
    10081 tcatggctta accctccaat cccaaagact gttgttgcac caccattttg ggcttgggga
    10141 catattccaa gaaacccaaa tcgttgagtt ttttgagacg gtaggaaacc gcgccggggt
    10201 tcatgtccaa gagtttgcct atttcggtca ggttcaagcc catactgcgg tagcgcagca
    10261 gcgcgagcat ttcgggcgcg gcttggaaat aggcgtcttc caatgcatcg atgcggtata
    10321 gcacggcatc aggcagggct tttgcctgtt tctccatttc gataaaatat cggcgggctt
    10381 ggcgtccttt gtcgttgcgc tccaccatgc acagctcttt tgccatgtcg agggataaat
    10441 aatacccctg aacagtttta tcacgctgtc caaagaaacc tgcttcggtg cggacaattt
    10501 tgtccacgcc gataaaatca agggcttgtg taaatccgta ttcttcaatg cgacgctgaa
    10561 tccatttagt tccacaccta agaacttatg caggtcatga gcatcaacca gtgcttgtgt
    10621 ttgattgtcc aattgacctg aaacagttgg gatgagttgg gtattcatgg taaaattatc
    10681 tttcatttca aggttgagat gacaaggaaa agaacgctct aacgttgctt ttccactatc
    10741 cccgataagc tgtcacttat cggggatttc catatcttgt gttgcacaag atatggaaat
    10801 tttaaattat ggtaattcta tttgtcaata ttcttttttg ctttcttttt gaatttttcc
    10861 gattccatat ctcctctaat tttcgatttt acccagtttg aaaaatctaa attttcagaa
    10921 aatttaatta aatcaagttc tttttcggaa ttgaacgata ctgttttcac tactcgcttg
    10981 agtgcgtatt tttcttgagc ctttttaatc gagtccataa tcttccttta aataggtttt
    11041 aaaacacgat tatggtaata ctaaaaacca ttttaatcaa gatgtattac aacaaaaagc
    11101 cgtctgaaat ggggtttaaa acctgtttca gacggccttt tttcatgccc tacataaaga
    11161 gagttgctgc tctgctttcc acctgttccg ccgatgtttt cccatcggcg gaattttctt
    11221 atttcatttt ttccttcatg actgatttaa cccagtttga aaagtcggga atttcttttt
    11281 gcataaaatc caccaactct ttctctcttt ccaaatggaa agaaactgtt ttcatcagcc
    11341 gcttttggtc atactttcgc tgatattcag cttgttttgc ttttagcgtt ttttcatcag
    11401 gcataggcaa tccttgattt ttaaaatcgt tgtagtaaaa tggcttgcag aaaggttggc
    11461 gaaaggggcg cggtgttctg ccaccccttg tgtttaatca acttgcttag taagccggac
    11521 tacagcttaa aagcagaatg atgaaaaaaa caactttcgc cagttttgtc atttccacaa
    11581 gcctcctttc tatccgataa gcccccgaag ttgccgcttc ggggctttgc tttatcagat
    11641 aggcgtatta tctagtattt ctagaattta gtcaagctat tttttgattt tttctttgat
    11701 gattgattta acccattggg aaaaatcgat attttgggca tattccaaaa gttctttttc
    11761 cgtttcggca ttgaacgaga ctttcttaat cacccgtttc tgctcgtaac gctttcggtt
    11821 ttctgccagt ttttcatcaa ccataggcaa actccttgat tttttaaacc gtattttgta
    11881 agattgggac tggggacagc ggcgaccgcc cccagctggt cagtctttag taggcattag
    11941 cacttgccaa catgaaaact gcgaccaaga tgagaatttg aatagtgcgt ttattcatct
    12001 tctttctccc agttgagccc cgcttcggtg gggcttttcc cgtatcggcc ctcactacgc
    12061 cgatgaattg aattataaac gtggacacgt taaaagtcaa ccctttcccc aacaaaatca
    12121 aagaaaaatg ccgtctgaaa acagctcgaa tgctgtttca gacggcattt tttgttgtcg
    12181 ggcatcaatg ccctgccatt tgtgcggcgt tgcggttatc aagatatggt attttagtgt
    12241 gtatttttag aagaaattta ccatatatag tattaaaata ttatacggtt gttttatatg
    12301 gatttgattt tcttgaaaaa taaatcccat atgctaatat ttgcactatt tgggcgaggt
    12361 tgaaaaatga ataccgtcaa agaatatacg gcagtccgcg aacggctact caatgctgcc
    12421 gattatttgg aagaagtccg taaagaccgt aagacgggca atattgcgtc cgtggaattt
    12481 gtgccgccga aaataggggc tcggggttac ggcaaattca aagtccgtta caaaacctta
    12541 gtggcggttg atttatgagc ggggaaaaat ccgagaatct gccggccgat gagcagcaga
    12601 aaatgcttca ggagttttta gaacttcaaa aacaggatat tgcagtcaaa cagaaggagt
    12661 tggaaagccg taaggacgaa atccaatctc acgaacgcat tgcactggct accatcgaag
    12721 cgcaaaaaca gggtgaacag caacatggtg agattttcaa agaggtacac aaacgccgtc
    12781 tgaatgccgt gattgctatt tcttttctga ttgccgccgt gctgattact gcgttatggc
    12841 gcgataagga cagcgtcgct attgaaattg tcaaaatcgg cggggcggta gctttgggat
    12901 attttgcagg aatcaacaga ggtaaagcgc aaaccttgga aaagcaacgg cgcgaaaaag
    12961 acagtgatta gattgtagta aaaaaggtcg tctgaaacgt tttggcgaaa ctcgcaaagc
    13021 tcgttttcag acggc
//
LOCUS       DAWFIT010000055        12993 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFIT010000055 DAWFIT010000000
VERSION     DAWFIT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.055"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..548)
                     /locus_tag="WGQ85_001423"
     CDS             complement(<1..548)
                     /locus_tag="WGQ85_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0750829.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIPV"
     gene            complement(580..963)
                     /locus_tag="WGQ85_001424"
     CDS             complement(580..963)
                     /locus_tag="WGQ85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0750830.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1332..2036
                     /locus_tag="WGQ85_001425"
     CDS             1332..2036
                     /locus_tag="WGQ85_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0750831.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2100..2666)
                     /locus_tag="WGQ85_001426"
     CDS             complement(2100..2666)
                     /locus_tag="WGQ85_001426"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0750832.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2732..3148)
                     /locus_tag="WGQ85_001427"
     CDS             complement(2732..3148)
                     /locus_tag="WGQ85_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750833.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3200..4099)
                     /locus_tag="WGQ85_001428"
     CDS             complement(3200..4099)
                     /locus_tag="WGQ85_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0750834.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4130..5587)
                     /gene="der"
                     /locus_tag="WGQ85_001429"
     CDS             complement(4130..5587)
                     /gene="der"
                     /locus_tag="WGQ85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0750835.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQTAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5741..6370)
                     /locus_tag="WGQ85_001430"
     CDS             complement(5741..6370)
                     /locus_tag="WGQ85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0750836.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6371..7666)
                     /gene="hisS"
                     /locus_tag="WGQ85_001431"
     CDS             complement(6371..7666)
                     /gene="hisS"
                     /locus_tag="WGQ85_001431"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0750837.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YRQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7793..8335)
                     /locus_tag="WGQ85_001432"
     CDS             complement(7793..8335)
                     /locus_tag="WGQ85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0750838.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8731..9267)
                     /locus_tag="WGQ85_001433"
     CDS             complement(8731..9267)
                     /locus_tag="WGQ85_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750839.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9435..9635)
                     /locus_tag="WGQ85_001434"
     CDS             complement(9435..9635)
                     /locus_tag="WGQ85_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750840.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(9750..10169)
                     /locus_tag="WGQ85_001435"
     CDS             complement(9750..10169)
                     /locus_tag="WGQ85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750841.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(10227..10592)
                     /locus_tag="WGQ85_001436"
     CDS             complement(10227..10592)
                     /locus_tag="WGQ85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750842.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(10574..11119)
                     /locus_tag="WGQ85_001437"
     CDS             complement(10574..11119)
                     /locus_tag="WGQ85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750843.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(11282..11553)
                     /locus_tag="WGQ85_001438"
                     /pseudo
     CDS             complement(11282..11553)
                     /locus_tag="WGQ85_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12115..12587)
                     /locus_tag="WGQ85_001439"
                     /pseudo
     CDS             complement(12115..12587)
                     /locus_tag="WGQ85_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12676..12900)
                     /locus_tag="WGQ85_001440"
     CDS             complement(12676..12900)
                     /locus_tag="WGQ85_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750844.1"
                     /translation="MMKNKYILTKAAIGITAISIFYLLNQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
BASE COUNT         3310 a         3072 c         2978 g         3633 t
ORIGIN      
        1 accggtattt tcttaccgta ttctgcgatt gcctgtcgga aaatgccgat caacctgcct
       61 ataacggcat tttcgccaaa ttcgttcaga cagttttccc taagtcgggc aggttcaaaa
      121 tcagagtggt gttcatacat tttgatgagt gcgtcggcaa gggcatcgtc gtcgtcaaca
      181 ggaaccaaat atccgttgcc gtctgaaaca atagattccg caccgccgca gcgtgtcgca
      241 atgacgggca atccttggga cagtgcttcg atatagacca cgccgaaggt ttcggtgcgg
      301 ctggcaagga cgaatgcgtc gctgttcctc atcaaatcca agactgcttc gggctgcaat
      361 gcgcccaaaa atgtaacggc atgggtaatg cccaagtctg ccgcctgctg tttcagccgc
      421 tgttcttcct gtccgctgcc gccgatgttc aggcgcagtt gcgggcattg tgccaacgcc
      481 cgggcaaagg cagtgagtag gacatcgtgt cctttgagac ggcgaaggtg cgagacggtg
      541 cagaacacga aatgcggttt gttgtttttt tctctttgtt caaacggtcg gttgaatatt
      601 ccgcccagta tgttggggga ggtactgcca ttcgcagccg tatttgtgtt gcaggacgtg
      661 tgcgaaatgg cggctgacag cgagcagtgc ggaggcgtgc gccgctgctt tttccatcgg
      721 ctgccattgg tggggacgga tcagaccgcg cgtgatggtg ctgctgtgtt ccgtgacgac
      781 ataggggatg ccgtattttt gggaaatttt ttgggcgagt atgccggcat agttcatgcg
      841 gtgggcgtga atcaggtcgg gcagcccgtt ttcgcggatg tagtgtttga aagctttcaa
      901 acccgcacac acccagcgga tgcagtcgat gtcgatgaac ggaaagcggg ggaagaaata
      961 tatgccgtgc catgcataga tgtccaaacc gctttgccga tattttgcaa aaccgtaagg
     1021 ccggtcagga tgcttgctgt ttctttccgc aagtagcgga acatcggtgc aagcacggcg
     1081 gttttgatgc ctttcctctg caatgccagt gcctgatttt gaaaaaaatc ccgtccacat
     1141 cctgttcgga ttgcggatac catgagggga tgacgaggac gtgcaagggt tcgggcatag
     1201 tgggattccg tatcggaaag gcggttatta taagacagac gcagaccgaa tatttaaatt
     1261 gttgccttac gctaatgcaa tttagcgcgc cgatatgcta gattggcggt tttataagta
     1321 aggatacgga tatgttgcgt tctattttgg cggcttccct gctggcggta tcttttccga
     1381 cggcggctga agcattgaat tacaatattg tcgaattttc tgaatcggcg ggtgtcgagg
     1441 cggttcagga tacaatgtcc gcacgtttcc aagtgacggc ggaaggacgg gacaaaaatg
     1501 ccgtcaatgc cgagtttgtt aaaaaattca acaatttcac cagaaaatca aaaaatggta
     1561 gctttaaaac cgaattggta tcgcgcagtg cgatgccgcg ctatcaatat accaacggca
     1621 gacgcattca aacaggttgg gaggagcgtg cggaatttaa ggtcgagggt agggattttg
     1681 atgcgttgaa ccgttttatt gccgatgttc aggcagatgc cgcgttggaa tatacggatt
     1741 tccatgtgtc gcgcgaacgc cgcaacgagg tcatcgatca ggtcagcaag gatgccgttt
     1801 tgcgtttcaa ggcgcgtgcc gaaaagttgg cgggcgtttt gggtgcgtcc ggttataaaa
     1861 tcgtcaaatt gaatttggga cacatcggca gccatatcgc gggaggggga gctgctcagg
     1921 caaaaatgct tcgtgccatg ccgatggcgg caagcgtcaa tatggagggt gcggattccg
     1981 ccgcgcctgg tgtggaggaa atcagcatca gcgtcaatgg gacggttcag ttctgatttg
     2041 aggtgaacgg caaatgccgt ctgaaacccg acgataaggg ttcagacggc atttatattt
     2101 caggctttgg gcagggtaac gccggtttgc cccatatatt tgccgttgcg gtctttgtat
     2161 gaggtttcgc agatttcgtc gctctcgaag aagaggactt gcgccacgcc ttcgcctgcg
     2221 tagattttgg cgggcagggg ggtggtgttg gaaaattcga gggtaacgta gccttcccat
     2281 tccggttcga acggggtaac gttgacgata atgccgcagc gggcgtaggt ggatttgccc
     2341 aagcagacgg tcaggacgtt gcgcgggatg cggaaatatt cgaccgtgcg cgccagtgcg
     2401 aaggaattgg gcgggatgat gcagcagtcg tcttcaacgg taacgaagtt tttcgggtcg
     2461 aagtttttgg gatcgacgat ggtgctgttg atgttggtga agattttaaa ttcgtttgcg
     2521 cagcggatgt cgtagccgta gctggacgta ccgtaggaga tgatgcgttt gccgtcggct
     2581 tctttgattt ggttcggctc gaaagggtcg atcatgccga attcttcgct cattcggcgt
     2641 atccatttgt cggacttgat gctcataatg ttttccttgt ttcttgcagt gttcggacaa
     2701 agcattgggg gatgccgtct gaaaacgggg cttatttgtt tttgggcagc ttcacttctt
     2761 taatcatgcc gttttcgcat ttcataatga agagggtttc gccgttttgc gagacggtaa
     2821 atctgccgtg tgccaagcct tttttgaatg tgcccgagag taccatgttg cggaatttgg
     2881 tactgtcgga attgaacggt tcgataaata tttcgcggtt ggcggcaacg gtataaacgc
     2941 cttgtccgtc gaatttgcca tttttaaacg aaccggtata gttgcgcccg tcttggcagc
     3001 gccatgtgcc tttgccggcg ggtttaccgt ctttgccgac atcgccttcg taggtgcagc
     3061 cgggttcttg ataggaagtc agaacggcgg acgcggcggg gagggcgaac atcatggcgg
     3121 gcagtaggaa tgcgagatgt ttaagcataa gggttattcc attggatttt ggctgacggt
     3181 ataaaagccg tctgaaaaat caatcttgcc agccgcccaa ataggaaacc agctcttcca
     3241 acatgatgct gacggattcc gccatcagga tttgcgaggc gaaggcgaga ccggcggcat
     3301 cgtcgccgtt gctttcggct tcttcctgca atacgtcgag gtattggatg cgcttgagtg
     3361 tgaagtcttg cgtgaggata aaggcaattt gttcgcgcca caccaagccc agttgggtaa
     3421 cggttttacc gtttttgacg tgttgaacca cttcgtcggc ggttaaatct tgtttggata
     3481 ctttgacgac gggaacaata tcgcccgtgc ctttgagttc gcaatcgctg tccaattcaa
     3541 aaccgccttc gcaatgccct tgcaacagcc agccggtcat cagggaagag ggcgattgtt
     3601 tggtattcgg cagcgaggct tccaaaccgc ccaaagcttc gcgcagcttg gtcaggatgt
     3661 tttccgcttt ggcggaagcc gcgttattga cgagcaggta gccgtggcgg gtgttaaaca
     3721 ccgcttctgt acggctgctg cgggtaaacg ctcggggcag caggtcgtct gtaatttgct
     3781 ctttaagctc ttgtttttct ttgcgaccga cattgcgggc ttcattgttt tggatttccg
     3841 ctaccttctc ttccaaaata tcgcggatga cgccggcagg caggactttt tcctcttttt
     3901 tcagggcgac gcgcaaggta aagtcggcag ggaaaacgag ttccggggag aatgaaaccg
     3961 gtgcggtaaa gccttcgctg aaccagtcta agccttggca atgggtaaat tcagcttcag
     4021 caagtttgtc ggcaagtacg tccgcctcag gcagcttttc tttgttgagc ggataaaaac
     4081 taatctgctt gaaccacata atgtttcctg ttgtttgaaa tgtcgggaat tatttgctga
     4141 attgtttttt cacactgact ttggttttct tcttgaagcg gtttttctct tctttgcggc
     4201 cttcgcgttt ctcaatacgg ttgctcaggc tgacgcggcg cagcggtttt ttcttcggtt
     4261 tgtcgtcggc gttttcatac gggttttccg aaacattgta ttgaatccgc aacggcgtgc
     4321 cttgcaggtt gaaggctttg cggaacgttt gggtcagata gcgcgtatag ctgtcggaaa
     4381 tcgcgtgcag cgaattgccg tgtaccacaa ttacgggagg gttcatgccg ccttggtggg
     4441 cataacgcat tttcggacgc accaagccgg cacgcggcgg ttgctgacgc tcgattgcgc
     4501 tttgcaggac gcgcgtgatt ttcggcgtcg gcatcttaat catcgccgcg ttgtaggcag
     4561 tctgaatgct gtcaaacaaa ccgtctatac cgcgctcttt caatgcggaa ataaagtgga
     4621 acttggcaaa atcgaggaaa tacagtttgc ggttgatatc gcgtttcact tgctcgcgac
     4681 gttcttcgct gatgccgtcc catttattga ccgccaccac caaagcacgg cctgcctcca
     4741 aagcgaaacc tgcaatcgtc gcatcttggt cggcgatgtc ctgctgcgcg tccaatacca
     4801 aaacagcgac gtttgccgct tcaaccgcct gcatcgcttt gataacggag aatttttcca
     4861 ctgcttcatc cactttgccg cgacggcgca cacctgcggt atcgatgatg gtaaacggtt
     4921 tgccttcgcg ctcgaaatcg atatggatac tgtcgcgcgt cgtacctgcc atatcgaagg
     4981 cgatgacgcg ctcttcgccg agaatggcgt ttaccaaggt tgatttaccg acatttggac
     5041 gaccgataac ggcaaaaaca ggatgtttcg cctcttcctc ttctttttca ggctcaggga
     5101 aggtttccaa aatatcttca atcagataat acacgccgtc accgtgcgca cctgaaataa
     5161 cataagggtc gcccaaagca agttcgtaga actcggcggc aagcacggcc ctgttgccgc
     5221 cttctccttt attcacggcc aaataaacgg ggcgcgggct ttggcgcaaa cggtcggcaa
     5281 tgattttatc ttgcggcgtc agaccggtac ggccgtccac caaaaacaca accgcatcgg
     5341 cttcatcgac agcctgtaag gtttgttttg ccatttcgtg caaaatgccg ctgtccacaa
     5401 ccggctcgaa accgccggta tcgatgacca aataaggttt gctgccgact ttgccgtgtc
     5461 cgtaatggcg gtcgcgggtc aggccgggca ggtcgtgtac gagtgcgtct ttggtgcgcg
     5521 tcaaacggtt gaataaagta gatttgccga cgttggggcg gccgacaagc gcgatggttg
     5581 gtttcatgat tgagtctttc tgtgtcaagt gccgttcggg agaactgaac acgagcaggt
     5641 gtccgttgga cacggtaaat aggttcttca cggcaagagg ccataaagag ggagggtaaa
     5701 atgccgtctg ataaataatg gacggcattt cagacggcat ttatttcagc gaatcaagtt
     5761 tcatttgaac caattcgcga ccgacagaat cttgaggcat tttttctaaa gcctgtccgt
     5821 agttttttaa ggcttcctgg ctttttccct gtgcggcata gacatcgcct ttggtttcca
     5881 tcagcagggg ggcgaagtcc gcttcaaccg gcgtgtcgag tgcggcaagc gcggcatcgt
     5941 attttttttg ttgcaacaac acaacgccca gacgctgcgc cgccaacgcc tggatcaggc
     6001 tgtctttttg gttggacaat acccatttca aatggccttc ggcaacatcg taacgctgcg
     6061 cgtcaaattc ggttgctgcc gccatcagcg tggcttgggc ggcggaaatg gaatgggggt
     6121 agctttgttg gagcttggcc aattcggcat tgatttcgct ttgcggggct ttgttttgcg
     6181 ccttttccac gatgtttgcc agcaccgccg ccgcttcctg attttgggaa gccgcacggt
     6241 tttggtaaac cgtgtatccc aagtagccga gtgccgccaa aatcagcacg gcaaacagcc
     6301 atttgcccgt ggttttccaa aaatatttaa agttgtccaa ctcttgttgt tcttccaaat
     6361 gggctgccat ttatgcgttc ttccattgtt gtaaagtgtt tgtcaaatca gcggcggcga
     6421 cggtttgctg accgtgtgcg ccgttcatat ctttgagcgt aaccgtaccg ttcgccagtt
     6481 cgtcttgcgc gacaatcagg gcaaagcgtg cgccgctgtt gtcggctttt ttcatttgcg
     6541 ctttcaggct ctgatagccg gaatgctgca tcacattgaa accttgcgcg cgcaaggctt
     6601 gtgcgtattt catcacctgc aagtccgccc cttcgccttg gtgcattgca tagacatcag
     6661 gcgcggcgtt tacttccagc gagccgtatt cgctcaccaa aagcagcagc cgctcgatgc
     6721 ccattgcaaa gccgatggac ggcgcaggct taccgccaag ttcctcaatc aaaccatcgt
     6781 aacggccgcc gccgcacaca gtcgcctgcg cgccgagttt gtcggttgtc cactcaaaaa
     6841 ccgtctgatt gtaataatcc aaaccgcgaa ccaagcgcgg attttcaata tattggatac
     6901 ccaaaccgtc caacatcgcc ttgaagcgtg catagtggtt ttgcgaagcc tcgcccaaat
     6961 aatccaccaa acgcggcgcc gcgttgcaga tttcctgcaa atcagggttt ttcgtatcca
     7021 aaacgcgcaa aggattggtt ttcagacggc gtttgctgtc ttcatccaat ttatcttcat
     7081 aacgggtcag atattcaacc aatgccgcac ggtgtgccgc gcgttcctca cggttgccca
     7141 aactgttgat ttccaaagtc agatattcgc ggatacccaa tttttcccat aagtcggcag
     7201 acatcgcgat gatttccgca tcaatatccg gaccttcaaa acccaaagct tcgataccga
     7261 cctgatggaa ctgacgataa cggccttttt gcggacgctc gcggcggaac atcggtccca
     7321 tataccacag cttttgcggg ctgttgtaga gaagattgtg ttcgaccacc gcacgcagac
     7381 aggaagccgt accttcagga cgcaggctca aactcaaaga atcgtttgaa tcggaaaagg
     7441 tgtacatttc cttgccgacc acgtcggttt cctcgccgat ggagcggaca aacaaaccgg
     7501 tttgctcgac aatcggcgta cggatttgcc gataaccgta agcgcgcgtc caacggttta
     7561 ccgtatcctc aaacgcctgc caaaacgcag ccgtcagttt gaaatctttt tgctcgacag
     7621 gcagaaggtc gttcatacct ttgacggatt ggattttttg tgccatttca agtaaaaatg
     7681 cttaaatcaa attgcgggcg attatagcgg atttttaagg gcttgtgagg atggagatgg
     7741 tttgcagacg ggtaaaaatg ccgtctgaaa cagttttcag acggcatttg acttactctg
     7801 cacgtgcttg cctgatttgt ccgactgcaa actctgtctg ccgcttggga tgatgtgtga
     7861 aaaacaattt atttgaaatt gtctcggctt ttttcggcac gaccgccaaa atcttacctg
     7921 ccaaatttcc ctcacgggtt tgccaagcat ccaaaaactg cgccctgctc attgaaacat
     7981 gacccagcga cgggtcggca agcaaaaccg tattgccgtc tatcccgcgc aataccgaaa
     8041 aatggtcgtc tttgcggtat ttcagataca cgatgacggg gattttcaac tgtgcgagct
     8101 gctcgaaaga cagggcatag cctttcgctt caaaacccaa atctggcata atgcgccgca
     8161 tatcctcaaa cgacgcgcgc atctgctcct tatccagctt tttcaacact tcttcttccg
     8221 tcagcgtttg cccgtaaaaa ttgttcaaaa gcgtcgccac cgaagccgcc ccacaggaaa
     8281 aatccaaatc ctgctttaca atattgaaat cccgcctttc tttccaactc tgcactttga
     8341 tttttccgta aacaacagga ttatcgttaa acatcggtgc agcattcaaa cgataagata
     8401 aagaaacgac aacacacgcc aacagaaaaa catatttgaa cttcatcata ttgtccacat
     8461 aaagggcagc ctgaaaatct ttcaggctgc ccttgtcaaa ttattcctag ctttcggctt
     8521 ttttggcaaa ccaaacaatc cgattacccg cataatactt tccatttatt gaaatccgac
     8581 aagccgcgcc caaaaaatgc catgcactgc cgatttccgc agtaatcttt gtatcgtttt
     8641 cttcaaattc caaatattca cccaataata aacttgaaac agaacgcgtg ggcagcaagt
     8701 gcccacccta cgcctattca aataatttga ttaaataaag aataaagaaa atgcatcagg
     8761 aacaaaatta taatctgcca cctgactcac accgctttta aagtaagggg catcaaaatc
     8821 aaaaccgcaa aaaaaataat ttttgcattg atttttaata gatttaaaat ttgaatatag
     8881 tgtttctcca gatttaaaaa gtttatcttt atctatatat ttgatgcttt ccctatccaa
     8941 aataaatatt tcaaacatta aaaaatcatt acatgaccaa gccaaatcat aaatttgatt
     9001 caaatgggta tcataaagat aaaaataata aggtttggga acaggtaaaa tatttaatgg
     9061 aaaaggagga ctaattttct taatatctga tgactgatat ccatatcttt ctatattctt
     9121 aaaaatttca tctttcttaa gataacaaga cattctgaca atcacaacct gttcatcgga
     9181 tatattatct atttgcatgg cgcgtataac acgccatgcc tgattaaaat tagtctccct
     9241 taccttagaa tctattatct tttccaaaga tgaaaatgcc ccttatcttg aactctccag
     9301 ttagaacctg gaagtttcgt acctcttaaa tgtgtattaa tatttcttat agtttcatta
     9361 aaatgccacg cgctgccgat ttcaacggta attttcgtac cgctttcttc aaattccagg
     9421 tattccccca ttagctaacg caaagaagca gacgccattt cggcttcgtt atgataaacc
     9481 cgccttccgt tgatatagac ttccgcccct gtccgctcca aattccaaaa attccgtact
     9541 gaaatttcca tatcccgata ttgtgcagac catgtttttt cgaaggtttt cataaaattt
     9601 cctatacctg tccaatcggc acatatcaat tgcattatta tagtggatta acaaaaatca
     9661 ggacaaggca acgaagccgc agacagtaca aatagtacag aaccgattca cttggtgttt
     9721 cagcacctta gagaatcgtt ctctttgagc taaggtgagg caacgccgta ctggtttaaa
     9781 tttaatccgc tatacatctc aatacgataa atattcctta agtcaaaatg caagcctgac
     9841 cgtaccttaa ctgtcaaaat tttattattt tttattgatt ttaaaacaat ttctgcaaaa
     9901 ttctcttcgc tttctctctt ttttagaagc acataagaaa aaataaaact tccccgatta
     9961 aattcataaa tatgtttcaa ccattcgcct cctctttctg taagacaaga ttcagtttca
    10021 ttcttcctta ttgtataaat atttccttca caaaatctga aataaatcca taaatccatc
    10081 ttatccataa ttaaagaaaa agtttcacct cgagattttg tcaacaattc gcaaggttgc
    10141 gatgttgcaa tcaaatagcc gaaagacatt ttttacctca tacatggtcg aaatcagttt
    10201 ctgttagttc agaatccatt ttttcgtcaa caactgaatc cgcatttttg aattaacgtt
    10261 ttcatcagct gccgtttatc taaaccggca ggttcagttt caaaataagc cttatatgaa
    10321 gactgtaagc atttcagaaa aagatcatca gaagacatat ctgccgaatc aaatacaact
    10381 gttttgattt tggtacttac ccaaaaccct ttttgctctt tttctactat acggaaattc
    10441 agaatatttc caaccgaatc aaaagcacgg taaacatcat ccatcaaatc ctgcggctct
    10501 attttctttt ccaattccga caatccttga aatatatcca aagacacatc ttcaaataga
    10561 aaaaaaggag gagttagaag cggtttttcc atgatctgtc cgtagatttt gattcccaag
    10621 ggcgatgacg accaattccc tgtccaggca aagtcttgcc cgtattatcc gtaactcgac
    10681 gatgataatg gggaaatttt ccaataggat gacctgttct attaccgaaa ggggctatcc
    10741 gcatattatt gccgatttta atctcacgtc catatttagc aaaggaaaca acctttcctg
    10801 cggcgcctac accaccagga attgcgccta atccgccagc aatagcaaca tctctaacag
    10861 aagctggtct gcctgtcgtt gcataactaa aaccatgctg tgtccacata ccaatggcag
    10921 caccacccaa gatagccaat ggaagaaacg ccctctcagt ctccttcatc tccttttgag
    10981 aaagttccgc caactgcatc ggcgcatctg cccgcgtgtg gaacacttgg tcttcaaatg
    11041 cctgattgtc caagccgttt gccattgcgg gggcaatcat agacagcatc attacggctg
    11101 cggtgatttg ttttttcata ataactcctt tggattacaa ggttggaaaa tcaaagccct
    11161 gcttagaacg tatgttgcac acccaatttc aataggtaaa tcagattgca aatccagcaa
    11221 tttgaatatt gtcattgttc cgtgcaaaag ggatttttat tgatgagttg tgtactgggt
    11281 ttcagcttgg ctttttagat aatcttttca caagaacata catcaatact gtccaaaaaa
    11341 aagatttgat aaatatattg caacatattt tattccacct ccatccgaaa cagaaaccaa
    11401 tatattttta attaatacat agctgattat tacatttatt attcctataa tatataagga
    11461 tcttgccaaa attttttttg attttatttt aggaatatct cttatccatg ctaaaataca
    11521 gcccaatgtc gaaaagaaaa taacagcaaa cactacctta cctcctctca cttccaacca
    11581 aaccgatagc aggtttggtt ggaagttggt ttatttatta tttaacggcg aatgtcagtg
    11641 ttcttacccg tagaacctgc ataaccattt atgccaccag caacagtccc cactgctaca
    11701 cctcgcatac cattaatcag aattcttcct attttttcga atccactatt tcttccaaag
    11761 atgcagcaaa cgcaggttga gcctagaccc aatctacgtt tgctgccgca tctgatccct
    11821 attgtttctt atccttacat cttcctgcct tgtcaatcaa ataaagacag aagactatac
    11881 aaaaactgac cgacatactg aaaataccta tccccttcca tgcaatctcc gcaccattga
    11941 cccaatagat tagactgaga aacaacaaag ccacagtata aatcaacgta aaaaatattg
    12001 cgtaaagcac aataaaggga acttttatct cacggttgtt ttttataata tattcagcaa
    12061 cttgatttcc gaatatacct gataaaaaat atagtattag atcatccatt tcgtttatct
    12121 tctatgtttt cccattgcgg cgctagaaga ctgatttaat gctgcaccat ttgcacgaat
    12181 aactgcatta ggaatagcat ttcccccttt ccaagcagaa cgggcaaaaa cactagtagt
    12241 aatccctgca ccgcgcaaca tcgcactgct atatccgccg cctatcatac caccagcggt
    12301 tgcagccaag gtacttcttg ttgaagcaaa ttgccctgtt ttaattttag aaatgccgtg
    12361 attagcccaa gcactaattg cccctcccat caaagcacct gccacaatag gaagaaacgc
    12421 cccctcagtc tccttcatct ccttttgaga aagctccgcc aactgcatcg gcgcatctgc
    12481 ccgcgtgtgg aacacttggt cttcaaatgc ctgattgtcc aagccgtttg ccattgcggg
    12541 gcgattatag acagcatcat cgtggcttca gtgatttgtt ttttcataac aaatttttag
    12601 ggaggagata atgttccatc aaaatcctcc tgaaaggctg cctgaaattt ccaagcagcc
    12661 tttttgattg cttaattaat cggatagtgc gacaaatata aagtgggcgg caattattgc
    12721 cgtaacggca gccataactg ttttattttc ttcaaaccaa agcaagttga gaaacataaa
    12781 catcaagaaa tatgatggtt cttcagtttt gccgctgctt tcttgattta gaagataaaa
    12841 tatagatatg gcggttatgc cgattgctgc ttttgttaaa atatatttgt ttttcatcat
    12901 cttgtatggt ttttcatgca agtttcaaca ataaaattcg gaataagata gagagatgtt
    12961 gatatatcag ataaatatat agtggattaa att
//
LOCUS       DAWFIT010000056        12920 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFIT010000056 DAWFIT010000000
VERSION     DAWFIT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.056"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            733..858
                     /locus_tag="WGQ85_001441"
                     /pseudo
     CDS             733..858
                     /locus_tag="WGQ85_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1009..2052)
                     /locus_tag="WGQ85_001442"
     CDS             complement(1009..2052)
                     /locus_tag="WGQ85_001442"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0750845.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(2049..2342)
                     /locus_tag="WGQ85_001443"
     CDS             complement(2049..2342)
                     /locus_tag="WGQ85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750846.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            2596..3750
                     /locus_tag="WGQ85_001444"
     CDS             2596..3750
                     /locus_tag="WGQ85_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0750847.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWQRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQEKHEEVKKLIYQLIELDFLELL"
     gene            3759..3965
                     /locus_tag="WGQ85_001445"
     CDS             3759..3965
                     /locus_tag="WGQ85_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750848.1"
                     /translation="MKNWKQFTFFVILVIACYQLLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            4170..4529
                     /locus_tag="WGQ85_001446"
     CDS             4170..4529
                     /locus_tag="WGQ85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0750849.1"
                     /translation="MKRKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDSTNYC"
     gene            4590..5306
                     /locus_tag="WGQ85_001447"
     CDS             4590..5306
                     /locus_tag="WGQ85_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0750850.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYALDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            5371..5826
                     /locus_tag="WGQ85_001448"
     CDS             5371..5826
                     /locus_tag="WGQ85_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0750851.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYE"
     gene            6549..6851
                     /locus_tag="WGQ85_001449"
     CDS             6549..6851
                     /locus_tag="WGQ85_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750852.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            7135..7374
                     /locus_tag="WGQ85_001450"
     CDS             7135..7374
                     /locus_tag="WGQ85_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0750853.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            7435..9180
                     /gene="hrpB"
                     /locus_tag="WGQ85_001451"
     CDS             7435..9180
                     /gene="hrpB"
                     /locus_tag="WGQ85_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0750854.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEEGK
                     SDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVH
                     KTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQ
                     YQSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAY
                     LNRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQ
                     FFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPR
                     HQFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGF
                     QTANTVYGFNLNYSF"
     gene            9289..12240
                     /locus_tag="WGQ85_001452"
                     /pseudo
     CDS             9289..12240
                     /locus_tag="WGQ85_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
BASE COUNT         3671 a         2835 c         3132 g         3282 t
ORIGIN      
        1 tttttgacac attttcatga tttcaaatcc cgttattccc aagtggacgg aaacccgata
       61 atgggaagcc gcagagactt atcgggaaaa cggcaagccc tccgccgtca ttcccgcgca
      121 ggcgggaatc tagaaataaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
      181 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
      241 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcacgg caatgatggg
      301 attttaaatt gcaggtattt accgggaaaa acggcaagct ctccgccgtc attcccgcgc
      361 aggcgggaat ctagaaatga aaaaccacgg tgttatcgga aatgattgaa accggagaaa
      421 tgacggaatc gaagcgtgtg gaaatgtgtc ggaaatcgct gaagccggat aaactagatt
      481 cccgcttttg cgggaatgac gggattttag gtttttggtt tgttttcgcg gcaatgacgg
      541 gattttaggt ttttggtttg ttttcacggc aatgatggga ttttaaattg caggtattta
      601 ccgggaaaaa cggcaagccc tccgccgtca ttcccgcgca ggcgggaatc tagaaatgaa
      661 aaaccacggt gttatcggca atgactgaaa ccggagaaat gacgaaattg aagcgtgtgg
      721 aaatgtgtcg gaaatcgctg aagccggata aactatagtg gattaaattt aaatcaggac
      781 aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac gctgtactgg
      841 tttaaattta atccactata gattcccgct tttgcgggaa tgacggtatt ttagattttt
      901 gttttcgcgg aataatgagc caaaagccgt ccgaacccaa aaaaaccgtc gctttcagac
      961 ggcatcaaaa aaccgcaggc acgatgcctg cggtttgccg tttgcgcttc aggggagcag
     1021 ggggattgcg cccaagccgt gtgtttcctt cagtccgaac atgatgttca tattttgcac
     1081 cgcctgaccc gccgcgcctt tgacgaggtt gtcgatgacg gaaaggacga cccacacatc
     1141 ggattgcgcc gcctgttgga tgctgatgcg gcagaggttt gcgccgcgca cgctgcgggt
     1201 ttcgggtgtc gaaccggcgg gcaggatgtc cacgaacagg ctgtcgcggt aatagtcgcg
     1261 caggatggtt tcggggcaga tgccgtctga aaggtggagg taaacggtgg cgtgcatacc
     1321 gcgtatcatc ggcgcgaggt gcggcgtgaa cacgaatcct tcggcgatgc cgtcctgaag
     1381 cccggcgatg gtctgcctga tttcgggcag gtggcggtgt ccagctatgc cgtatgcttt
     1441 gaagttgtcg ccgacttcgc acaacagcga accgacattg cctttcctgc ccgcgccgga
     1501 cacgccggat ttgcagtcgg caatcagcgg cataccgggc ttcagacggc attgccgcaa
     1561 cagcggcacg agcggcaggg atacgcaggt cgggtagcag ccggggttgg cgacgaggcg
     1621 tgcctgtgcg acggcttcgc ggttgagttc gctcaatccg tacacggctt gggaaacgag
     1681 gtcgggggcg gcgtgggtca tgccgtacca gtgttcccag gtcggaatgt cccgtatgcg
     1741 gaagtcggcg gaaaggtcga tgacgcgcac gccctgttca atcaggcgcg gcgcgtcttt
     1801 catggcgatg ccgttgggcg tggcgaagaa gacgatgtcg cattgttcca aacctgcctc
     1861 gtcgggcgtt tggaaggcga ggccgtacac gccgcgcaaa ctcggaaagt aatcggcaac
     1921 tgcagttccc gcttcgctgc ggctggttac ggcggcgact tcgacatcgg gatgggcggc
     1981 aagcaggcgc agcagttcca cgcccgtgta gcccgtcgcg ccgacaatgc cgactttgat
     2041 ttttttactc atggtttttc ctttgtgtgg ttggcgggta tgccgtccga acgctgtccg
     2101 acggcttgtc cgacggggtt ttgattggcg gcggaaggac ggtacacaaa gcgtttgaaa
     2161 agcgtgtcta atttaatggc atccgcctct gtttttaaaa cccagccctg ccgtccggaa
     2221 taaacataac cgtaggcgcg aaagtcggca ggatatttcg gtgcctgccc caaacccaaa
     2281 ccgtatgcgg cgaaagcggt ggtcgtggag aaggaaagca ggaggaggac gagggttttc
     2341 ataaggttcc aaaccgaacg gggcgatgaa gcccgattat agcaaaaggt cgtctgaaaa
     2401 ccgttttcag acgacctttt atttaattac aataaaaatg ttaacaacaa aaaacaaacc
     2461 gcttttttcc gtttggagac aaatttttaa ctaaaattgt caacaattcc ttgacgcaca
     2521 cacaaaaaaa acttcaatat ttgtaaatat atgtaacatt ataaatttta aatattttct
     2581 gagtaagggg aagtaatgag catccatctc gacttcggca ttagtcctaa aacgttccga
     2641 cagacttatc tgtatcaaaa gcccaagctc tttaaaggag cggttcggaa tctcgaatcc
     2701 gcatcttgga aagatatcaa cgagatatac caacgagcag acccaaccgc accgctgttt
     2761 catctgcgta aaaaaggcgc aatcgttcct aaagaagaat acgtcgaaag tttcgacgat
     2821 ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct acgaacatat gaagaatggt
     2881 gcgtcgttag tctataacca tattaacaac gagccgtttt cagaccatat cgcccgtcaa
     2941 gtcgcccgct ttgccggcgc acatactatt gttagtggat atcttgcttt tggcagcgac
     3001 gaatcttata aaaaccattg ggacacccgc gatgtgtatg ccatccagct tttcggcaag
     3061 aaacgttggc aacttactgc ccctgatttc cctatgccat tgtatatgca acagactaaa
     3121 gatactgata tttccattcc tgaacatatc gatatggata ttatccttga agcaggtgat
     3181 gtcctctaca tcccgcgcgg ttggtggcaa agacctatcc cgctcggctg tgaaaccttc
     3241 cacctcgctg tcggtacctt tccgcccaac ggctataatt acctcgagtg gctaatgaag
     3301 aaattcccca ccatagaaag tctgcgccac agtttctcag actgggagca agataggacg
     3361 cgtatcaacg atactgccgc acaaattgct gccatgattg ccgaccccgt caattacgaa
     3421 gccttcagtg aagacttcct cggcaaagaa cgtaccgata ccgcttttca tctcgaacag
     3481 ttcgcgaatc ccaacgctac tccgctttca gacgacgtca ggttgagact aaatgccaat
     3541 aatttggata cgttggaaaa gggatatttg attgggaatg ggatgaagat aagcgtagat
     3601 gagttgggga aaaaagtgtt agaacatatc ggtaagaatg aaccgttatt gttgaaaaat
     3661 ctactggtta acttcaatca ggaaaaacat gaagaagtta agaagttgat ttatcagttg
     3721 atagagttag attttctgga acttttgtga gggattctat gaaaaactgg aagcaattta
     3781 catttttcgt aatattagta atagcttgtt atcaattgct atatttttta tcagatatgt
     3841 ttcttctcga ctacattaac aaatatagtt ggaatttgaa ttttattcag ggtactttga
     3901 attttttttc aatatatctg ccatatgttt ttgttagccg aatttttagg aataccaacc
     3961 aatgaaagac taggctatga aatcaaaatt tgtgtataag ttactggtgc ttgcaaagta
     4021 cctgtatatt ttaatttgta tatcaatacc aatcgttttt atttatgaaa gctaccaagg
     4081 agatgatgtt aattttatga tttatattcg tgcttttatt attttttcca tattgggtgt
     4141 attgactcaa tattctatgt ctaatttttt tgaaaaggaa aaataacatc attgaactat
     4201 ccattgagaa tctggagcta atttatggcg gaggcttagg ggagagaatc cttgaaggag
     4261 gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg tattggtctg ggtgtcggta
     4321 tagtttctgg tggtattcct gccggaccgg ctattattct cggtggcaca gttggtgctg
     4381 ctgttggtgc tgtaactggt atgggtgttg cagcatatga gtattaccaa gaaactcagc
     4441 ccaaagggac agtaacggtc ggtccgattt ctgcttttgc tgtggacaaa tcaggaaacg
     4501 gctacactga cagcacaaat tactgctgat atttctcatc gtccagacaa tctctagggg
     4561 tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc caagcaaccc ttcttccgtc
     4621 ccgaagtcgc cgttgcccgc caaaccagcc tgacgggtaa agtgattctg acacgaccgt
     4681 tgtcattttc cctatggacg acatttgcat cgatatctgc gttattgatt atcctgtttt
     4741 tgatatttgg taactatacg cgaaagacaa cagcggaggg acaaatttta cctgcatcgg
     4801 gcgtaatcag ggtgtatgca ctggatacgg ggacaattac agcgaaattc gtggaagatg
     4861 gagaaaaggt taaggctggc gacaagctat ttgcgctttc gacctcacgt ttcggcgcag
     4921 gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt gaagaaaacg ttggcagaac
     4981 aggaactggg tcgtctgaag ctgatacacg ggaatgaaac gcgcagcctt aaagcaactg
     5041 tcgaacgttt ggaaaaccag gaactccata tttcgcaaca gatagacggt cagaaaaggc
     5101 gcattagact tgcggaagaa atgttgcaga aatatcgttt cctatccgcc aatgatgcag
     5161 tgccaaaaca agaaatgatg aatgtcaagg cagagctttt agagcagaaa gccaaacttg
     5221 atgcctaccg ccgagaagaa gtcgggctgc ttcaggaaat ccgcacgcag aatctgacat
     5281 tggccagcct cccccaagcg gcatgagaca gaacaaagct ggcttgaacg caccattgcc
     5341 gatatttctc aagaagtttt ggattttgaa atgcgctctg aacaaatcat ccgtgcagga
     5401 cggtcgggtt atatagcaat accgaacgtc gaagtcggac agcaggttga tccttccaaa
     5461 ctgctcttga gcattgttcc cgaacgtacc gagctatatg cccatctata tatccccagc
     5521 agtgcagcag gctttatcaa gccgaaagac aaggttgtcc tacgttatca ggcatatccc
     5581 tatcaaaaat tcgggcttgc ttccggcagt gtcgtatcag tagcaaaaac ggcactgggc
     5641 agacaggaat tgtcgggatt gggcatggta tcctccgatt tggcgaagag caacgaacct
     5701 gtttatctcg tgaaaataaa acccgacaaa ccaaccatca ctgcatacgg tgaggaaaaa
     5761 ccgctgcaaa tcggcatgac gttggaagca gacatcctac acgagaaacg gcgattgtac
     5821 gaatgagacc tttgcaataa cataggttac taaaatttta tgctcaatct cattttcaaa
     5881 atgcaaaact tttctgattt ttcctacttt ttgctcaata ttaggaaggt tttaggcaat
     5941 tgaaaatttt ttggcgcatt tttatgcgtc aaatttcgtt aacagactat ttttgcaaag
     6001 gtctcgaatg ggtattggag ccgatttata gtatatcggg caaactgtaa gacttgaggc
     6061 tgaaagataa agaagttgtc tgaaaccttt tcagacgacc agaagacaat atataaaaac
     6121 acaaacttca aaactgcctg aaaccatccc cttttacagg cactccgttt gagacctttg
     6181 caataacata ggttactaaa attttatgct caatctcatt ttcaaaatgc aaaacttttc
     6241 tgatttttcc tactttttgc tcaatattag gaaggtttta ggcaattgaa aattttttgg
     6301 cgcattttta tgcgtcaaat ttcgttaaca gactattttt gcaaaggtct ccgtctgtaa
     6361 aagcaaggat agggcatctg cccttttgat tgtttgatta acgatacaag gagtttcaaa
     6421 atgagagttt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact
     6481 atttgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc actatatgtg
     6541 ttcatgaaat gacttgggtc ggaggctcag gtaatgcgca acaaagttca tattattgcg
     6601 aaatttgcga atctgcaggg cttaacgata cgggaaatcc tgataaatct ttaggattgc
     6661 caaacaatac gttcagtaat ccgcctggtt ggggagctac aatcggagct ttagcaggta
     6721 gccgcatagg gatgcctgaa tttggtacgt ttgcgagcca tgccattgaa aatttcgact
     6781 ggtcatggta tcgacgttat agggaaattg ccgaaacgat tgaacgagaa tattcaggcg
     6841 gtttgcctta atagttgagg aggtcatgat gtttgccaaa cattatcaat tcatcgcact
     6901 cggcatcatg ctgcttcttt atatgttgat tctctatacg accgattttt ccaatctgac
     6961 gtattggatg ctgtttttta tctgttttat tacaggaaaa atattagctc gtttgttaga
     7021 gaaaagcttt aaataaaata gcagctagtc gcaaaaggtc gtctgaaacc ttttcaggcg
     7081 gcctttctaa aatacatcca acttcctaat ccctattttt caaaaaggaa atctatgccc
     7141 catctgcaaa acctgtcttt gggcttaaag aaaaagctgc ctgttatcct gcaaacagaa
     7201 atatcagaat gcggcttggc atgtctggcg gctgtggcgg gatttcatgg tttccatacg
     7261 aatttacgcg cactgcgttc aaaatactgt ccgagacctt tgcaaaattc cccaaaatcc
     7321 cctaaatgtc ttggtgggaa ttttggggaa ttttgcaaag gtctcattct ataactgtaa
     7381 atacttttaa atttatgaca aaatagtaaa tattgctaaa ataatattga tgtcatgaaa
     7441 ttttttcctg ctccatgtct gttggttatc ctggctgtca taccccttaa aaccttagct
     7501 gccgatgaaa acgatgcaga acttatccgt tccatgcagc gtcagcagca catagatgct
     7561 gaattgttaa ctgatgcaaa tgtccgtttc gagcaaccat tggagaagaa caattatgtc
     7621 ctgagtgaag atgaaacacc gtgtactcgg gtaaattaca ttagtttaga tgataagacg
     7681 gtgcgcaaat tttcttttct tccttctgtg ctcatgaaag aaacagcttt taaaactggg
     7741 atgtgtttag gttccaataa tttgagcagg ctacaaaaag ctgcgcaaca gatactgatc
     7801 gtgcgtggct acctcacttc ccaagctatt atccaaccac agaatatgga ttcgggaatt
     7861 ctgaaattac gggtatcagc aggcgaaata ggggatatcc gctatgaaga aaaacgggat
     7921 gggaagtctg ccgagggcag tattagtgca ttcaataaca aatttccctt atataggaac
     7981 aaaattctca atcttcgcga tgtagagcag ggcttggaaa acctgcgtcg tttgtcgagt
     8041 gttaaaacag atattcagat tataccgtcc gaagaagaag aaggcaaaag cgatttacag
     8101 atcaaatggc agcagaataa acccatacgg ttcagtatcg gtatagatga tgcgggcggc
     8161 aaaacgaccg gcaaatatca aggaaatgtc gctttatcgt tcgataaccc tttgggctta
     8221 agcgatttgt tttatgtttc atatggacgc ggtttggtgc acaaaacgga cttgactgat
     8281 gccaccggta cggaaactga aagcggatcc agaagttaca gcgtgcatta ttcggtgccc
     8341 gtaaaaaaat ggctgttttc ttttaatcac aatggacatc gttaccacga agcaaccgaa
     8401 ggctattccg tcaattacga ttacaacggc aaacaatatc agagcagcct ggccgccgag
     8461 cgcatgcttt ggcgtaacag gtttcataaa acttcagtcg gaatgaaatt atggacacgc
     8521 caaacctata aatacatcga cgatgccgaa atcgaagtgc aacgccgccg ctctgcaggc
     8581 tgggaagccg aattgcgcca ccgtgcttac ctcaaccgtt ggcagcttga cggcaagttg
     8641 tcttacaaac gcgggaccgg catgcgccaa agtatgcccg cacctgaaga aaacggcggc
     8701 gatattcttc caggtacatc tcgtatgaaa atcattactg ccggtttgga cgcagccgcc
     8761 ccatttattt taggcaaaca gcagtttttc tacgcaaccg ccattcaagc tcaatggaac
     8821 aaaacgcctt tggttgccca agacaagttg tctatcggca gccgctacac cgttcgagga
     8881 tttgacggtg aacagagcct ttccggagag cgaggtttct actggcagaa cactttgggc
     8941 tggcatttcc atccgcgcca tcaattctat ctcggcgcgg actatggtcg ggtatcgggc
     9001 gaaagtgccc aatatgcctc gggcaagcgg ctggcgggtg cagtggccgg cttcagaggc
     9061 agccataaag taggcggtat atttgcttat gacttatttg ccggcaagcc gctgcacaag
     9121 cccgaaggtt ttcagacggc caacaccgtt tacggcttca atttgaatta cagtttctaa
     9181 cctctgaatt ttttactgat atttagacgg tctttcctta tcctcagacc gtcaaacttt
     9241 acctacgtac ttggcgcgca gtacgttcat cttcaaaatg gaatagacat gaataaaggt
     9301 ttacatcgca ttatctttag taaaaagcac agcaccatgg ttgcagtagc cgaaactgcc
     9361 aacagccagg gcaaaggtaa acaggcaggc agttcggttt ctgtttcact gaaaacttca
     9421 ggcgaccttt gcggcaaact caaaaccacc cttaaaacct tggtctgctc tttggtttcc
     9481 ctgagtatgg tattgcctgc ccatgcccaa atcaccaccg acaaatcagc acctaaaaac
     9541 cagcaggtcg ttatccttaa aaccaacact ggtgccccct tggtgaatat tcaaactccg
     9601 aatggacgcg gattgagcca caaccgctat acgcagtttg atgttgacaa caaaggggca
     9661 gtgttaaaca acgaccgtaa caataatccg tttctggtca aaggcagtgc gcaattgatt
     9721 ttgaacgagg tacgcggtac ggctagcaaa ctcaacggca tcgttaccgt aggcggtcaa
     9781 aaggccgacg tgattattgc caaccccaac ggcattaccg ttaatggcgg cggctttaaa
     9841 aatgtcggtc ggggcatctt aaccaccggt gcgccccaaa tcggcaaaga cggtgcactg
     9901 acaggatttg atgtgcgtca aggcacattg accgtaggag cagcaggttg gaatgataaa
     9961 ggcggagccg actacaccgg ggtacttgct cgtgcagttg ctttgcaggg gaaattacag
    10021 ggtaaaaaac tggcggtttc taccggttct cagaaagtag attacgccag cggcgaaatc
    10081 agtgcaggta cggcagcggg tacgaaaccg actattgccc ttgatactgc cgcactgggc
    10141 ggtatgtacg cagacagcat cacactgatt gccaatgaaa aaggcgtagg cgtcaaaaat
    10201 gccggcacac tcgaagcggc caagcaattg attgtgactt cgtcaggccg cattgaaaac
    10261 agcggccgca tcgccaccac tgccgacagc actgaagcat caccgactta tctctccatc
    10321 gaaaccaccg aaacaggcgc agcaggcgca tttatctcca atggtggtcg gatcgagagc
    10381 aaaggcttat tggttattga gacgggagaa aatatcagct tgcgtaacgg agccgtggtg
    10441 cagaataacg gcagtcgccc agctaccacg gtattaaatg ctggtcataa tttggtgatt
    10501 gagagtaaaa ctaatgtgaa caatgccaaa ggctcggcta ctctgtcggc cggcggtcgt
    10561 actacgatca atgatgctac tattcaagcg ggcagttccg tgtacagctc caccaaaggc
    10621 gatactgaat tgggtgaaaa tacccgtatt attgctgaaa acgtaaccgt attgtccaac
    10681 ggcagtattg gcagtgctgc tgtaattgag gctaaagaca ctgcacacat tgaagcgggt
    10741 aaaccgcttt ctttagagac ttcaaacgtt gcttctaaca tccgtttgaa caacggtagc
    10801 attaaaggcg gaaagcaggt ggtattgatg gctgatgacg acattcaggc aaaagcctcc
    10861 cacctgaatg cgtccggcaa tctgtatatt catgcaggta aggatctgga tttgaatgcc
    10921 gataaagact tatcaacaca aagtatcagc ctgagggcag acaacaccgc cctcatcagc
    10981 agcaacggta acaccttgac tgcagaaaag aatctggata ttcaggcagg tagcttgagt
    11041 gtgcgtcaaa gtaatctgca atccagtggc ggtaatgtgc agatgagcgc gactaaaggc
    11101 aacatcagct tgaatcagag ttggataaat gccagtcaga atattgatac ggcagcactt
    11161 caaggcaata ttatttcaga cggtctgact gctgttgccg aagttggacg cgtatctctt
    11221 cttgccaacg gcaatgttga tttcaacggt ctaaatacct tgattgcgga aggagacatt
    11281 aatgctggtt cagtcggtaa aggccgtctg aaaatggaca ataccgatat ttatgcttct
    11341 gcaggcgatg tgaaactggt tgccggaggt caattagatt taggcaacgg caccgttaac
    11401 ggcggtcata tcagcttaga cagcaataaa ggcagcatgg tggtgcagaa tgtacacctg
    11461 aatgcccgcg catcactgaa agtggatgcg gatcaaactt taaccattaa taacagcaag
    11521 ctcaattccg atcacaatac ccagattaat acaaatcatg gtcatatgac gcttaatcag
    11581 cttgatgctc attcacgtcg tcatatgagt atcagtgcac agggtaaagg caaaggaaaa
    11641 gacagcggtc aaattttaca aaacgaccag caaaacagta aaagtacttt ggcggcagat
    11701 ggtgtattgt cattgaacag cagcgcatta caggttttgg acaatactac cctgcgcggt
    11761 ggcgcgataa acatcaaagc tggcggaggc atcatcaaac gggggcatat cgattgggaa
    11821 acccaagata cggcaactat gcgttcagcg gaactgaaac cactgtccgg tatgatgtcg
    11881 atagaatcgg gcggtgataa ccctttaacc gttgaacccg gtaaccgtat cgtttctgca
    11941 ggtgatttgg ctgtgaaaca caacggcaca ttccaaatca gtgccagagc aggaaataac
    12001 ggcaatccga gtgcgcaaac agccagcgtt tcagctaaag gcaatatcgg gattgtggcg
    12061 ggagaggtgg atattgatgc agcaaatatt gccgcaggga aagatttggc tttagtggca
    12121 actaaaggga atatttcact aaacagtatt aggaatacgt ttagtaacta tcaattgaaa
    12181 acagataaac acaatatcac acagcaactt actgatgtag agcaggagct tagtaagtta
    12241 acgagcgatc ctaaatatcg taaagcacag gatcttccac aaatgttgag gcgtaaatat
    12301 aagaggcgtg acaaagtatt cggtgatagt gaggccagat tacgtggttt acgagcaaag
    12361 ataaatgctg ctgatgaggc ttgggcagaa cgccaatccc cagtaaaagc gttacttgaa
    12421 cgtaaacaat tgttacaaca agccctttta acagtttccc aaccgggtag tggtcacgaa
    12481 aaccaaggta gcacccttag cggtcaaaat atcaaactgc tcgctgcagg tggtatccgt
    12541 atccagggct ccaaagttgc tgccacccaa caggcaaata tccaagctgc aggattttta
    12601 cctgcaccag ccgcagagga actgcaagaa ggtcgacttc aatcagccat tgacatcagc
    12661 ggtgtacttg atacttttga atatggtcag caaggcagcg ataaatacgg ctatgccatt
    12721 ttcagcaggc catctgaaat ttcaggaaaa acaggtgtta ccctctctgc accaaatgcc
    12781 aatgagaaca gccgcatcag tctgagtgcg gcaaacatcg aagctgaaaa cggcaaaatc
    12841 aaaattcaat cttacggtga ccaatattac tatgcgagac agagcgaact ctataccttt
    12901 gaacgccgca gctacaaaac
//
LOCUS       DAWFIT010000057        12906 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFIT010000057 DAWFIT010000000
VERSION     DAWFIT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.057"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(95..403)
                     /locus_tag="WGQ85_001453"
     CDS             complement(95..403)
                     /locus_tag="WGQ85_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750855.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(387..689)
                     /locus_tag="WGQ85_001454"
     CDS             complement(387..689)
                     /locus_tag="WGQ85_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0750856.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(763..3126)
                     /locus_tag="WGQ85_001455"
     CDS             complement(763..3126)
                     /locus_tag="WGQ85_001455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0750857.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAGTAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPQDAPVGVNIREYLDLDDTVFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQSVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSAAQFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTEHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAVNKLVQEIGVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3162..3680)
                     /locus_tag="WGQ85_001456"
     CDS             complement(3162..3680)
                     /locus_tag="WGQ85_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750858.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIVP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3687..4751)
                     /locus_tag="WGQ85_001457"
     CDS             complement(3687..4751)
                     /locus_tag="WGQ85_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0750859.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4928..5920)
                     /gene="pheS"
                     /locus_tag="WGQ85_001458"
     CDS             complement(4928..5920)
                     /gene="pheS"
                     /locus_tag="WGQ85_001458"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0750860.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6266..6625)
                     /gene="rplT"
                     /locus_tag="WGQ85_001459"
     CDS             complement(6266..6625)
                     /gene="rplT"
                     /locus_tag="WGQ85_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0750861.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6638..6835)
                     /gene="rpmI"
                     /locus_tag="WGQ85_001460"
     CDS             complement(6638..6835)
                     /gene="rpmI"
                     /locus_tag="WGQ85_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0750862.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6982..7503)
                     /gene="infC"
                     /locus_tag="WGQ85_001461"
     CDS             complement(6982..7503)
                     /gene="infC"
                     /locus_tag="WGQ85_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0750863.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7521..9434)
                     /gene="thrS"
                     /locus_tag="WGQ85_001462"
     CDS             complement(7521..9434)
                     /gene="thrS"
                     /locus_tag="WGQ85_001462"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0750864.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9487..9563)
                     /locus_tag="WGQ85_001463"
     tRNA            complement(9487..9563)
                     /locus_tag="WGQ85_001463"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9527..9529),aa:Val,seq:gac)
     gene            9742..10857
                     /gene="tgt"
                     /locus_tag="WGQ85_001464"
     CDS             9742..10857
                     /gene="tgt"
                     /locus_tag="WGQ85_001464"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0750865.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            10867..11949
                     /locus_tag="WGQ85_001465"
     CDS             10867..11949
                     /locus_tag="WGQ85_001465"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0750866.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12140..12608
                     /locus_tag="WGQ85_001466"
                     /pseudo
     CDS             12140..12608
                     /locus_tag="WGQ85_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(12605..12895)
                     /locus_tag="WGQ85_001467"
     CDS             complement(12605..12895)
                     /locus_tag="WGQ85_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750867.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3285 a         3416 c         2850 g         3355 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactataa ttatttttta gcgtgtaaaa
       61 caaaaccggc tgcatacctg caaccggcct caaatcagca caattcctta tccaaatccg
      121 ccaacagctc tttcagaccg tctccgattt cctgaagcgt aaccggacgg ttgccgtccg
      181 aacccgactc cgcaccttct gcatacggtg caaacgtgct ctgcaatttc aacagtttca
      241 ccacatccaa acgggtgtag cgttcgccgc cgtaaccgac aaccacaccg taatcatgct
      301 gccattgcgc aaaaccatag gggctgattt gcaacagtcc gcacaactcg tccagcgtga
      361 aatagcgttt tgcgggaacc ttcaaatcaa cgttgtttgt catagtagtg ttccaccatg
      421 cttttgagtt tctggctggc atggaaagtt accacgcgac gggcggtaat cggcacttcc
      481 tcgccggttt tcggattgcg gcccgggcgt tgcggcttgt cgcgcaactg gaaatttccg
      541 aaaccggaaa ttttgatttc ttcgccgctt gccaaagtgc tgcggatttc ttcaaaaaag
      601 agttcgacga tttctttggc atcgtttttg gtgacgttgc tgactttatc caccaaaata
      661 tcggccagtt ctgctttagt tagtgtcata tgattacctt ctgttgtaat agttgtggat
      721 tattaccaat ttttatttaa aattcaagcg aatatttatt ttttaactgc gaagccgcgc
      781 ccctgccgcc gttgccgcgc caatcagttt tccgataagc ggctcgaccg cctcatccgt
      841 cagcgtgttt tccatatcct gcaaaatcac tttgaccgcc acgctcttca tcccttcggg
      901 cagacccgtg ccgcgataca cgtcaaacac gccgatttcc tgtaccaact tgtttaccgc
      961 gcctttcaag acaagcagca aatcatcatg gctcatagct tccggcatca caaacgccaa
     1021 atcgcggcgc accggctgga atttcgatac ggcccgatag cgcgttttcc cgcattccaa
     1081 cacggccgcc atatcgattt caaataccag cggcgcttgc ggcaggtcgt atttttgcag
     1141 ccatttcgga tgcagttcgc cgacaaagcc gatgactttg ccgtctgaaa cgatattggc
     1201 ggcgcgtccg gggtgcaggg cgggatgttc ggttttgaca aactcgactg ctttgttttt
     1261 caacagattt tccacatccg ccttgatgtc gtaaaaatcc gcattacgcg ttttctcgcc
     1321 ccattgctcg ggcatcgccg caccgtacca caaaccgccg atacgttcgt tttgaacaaa
     1381 ttgagcagcc gaacccttgc cgaacacgcg ggcgatttca aacacgcgca cgcggttttg
     1441 cttgcggttc aagttgtttt gcagaatttc caccaagccg ccgatgagcg tggaacgcat
     1501 cacggcatac tgcgccgcca gcgggttttg caggcggatg gggtcggcgt tggcggcaaa
     1561 atcttgttcc cactgctcgt caacgaaggc atagctgacc acttcgcggt agccgcgcgc
     1621 cgccatttcg ttgtaaacgg caaaacgcgg acggcgtgtt tcgggcagtt ccagcatttt
     1681 cagacggcct gacgtgtaat cgtcggggat gttttcatag ccgtaaacgc gtccgatttc
     1741 ttcaatcaaa tcagcctcaa tttcgatgtc gaaacggaag ctcggcgcgg taacgcggaa
     1801 gccttccgcc gttttctcgg gctgcaaacc caagtgttgc aaaatggttt ccacctgttc
     1861 ggcagggatg tccacaccca acacggtttt cagacggccc aaacgcaatc cgacctgctt
     1921 cgcttcaggc aattcgcctt gcgcttccac catttctcct gccgcaccgc cgcagatttg
     1981 caacaccaat tcggtggcgc gttcaatggc atccgcctgc aaacggtaat ccacgccgcg
     2041 ctcaaagcgg aacgaagaat ccgaaccgaa accgtactgg cgcgatttgc cggcgatgat
     2101 ttcgggcgca aaccaagccg cttccagcac gatattttgc gtgccgtctg aaaccgcgct
     2161 cgccgcgccg cccattaagc ccgccaaact caacacgcct ttttcgtccg ccacgaccag
     2221 cgtattttca gacagggaaa cggttttctc gttcaggcat tccagcgttt ccccttcgcg
     2281 cgcgcggcgg atgtgcaggc tgccggaaag tttgtcggca tcgaaaacgt gcatcggctg
     2341 gccgatttcc agcattacat aattgccgat gtccaccagc acggaaatac tgcggatgcc
     2401 gctgcgctcc aaacgttgtt tcatccaatc gggagtagcc gctttcgcat tgacattttc
     2461 aataacgcgg ctgataaaac ggccgcaatc ggcaggcgcg ttaatctgca cggactgttt
     2521 tcgactgccc gtgatcggcg cggtatggat ttcgggctgc ctgaacgcgc accccgtcaa
     2581 tgcggacact tcgcgcgcaa tgcctttgat gctcaggcag tcggcacggt tcggcgtaat
     2641 tttcagcgta aacaccgtgt cgtccaaatc caaatattcg cgaatgttta caccgacagg
     2701 agcatcttgc ggcagaatgt gcaggccgtt cacaccgtcg tcgggcagac cgagttcgtc
     2761 ggtggaacac agcatcccgt tcgacaccac gccgcgcatc ttggtcggct tgattttgaa
     2821 attgcccggc aacaccgcgc ccggcaacga acacggcact ttgatgcccg ctttcacatt
     2881 cggcgcaccg cacacaatct gaaccaactc acccgtaccc gcatcgactc gggtaacgtt
     2941 caaacggtct gcatcaggat gtttttcaac agattttact tcggcaacaa ccacgcccgc
     3001 aaaggcaggc gcggcagtcc cggcttcttc cacttccaag ccggacatcg ttaacaggtg
     3061 ttccagctta tcggcggaaa gttcggtatc ggcttgggtt ttcagccatg agtaggagaa
     3121 ttgcatgtta tgttctcaat taactaatta actttagatt ttcaagagct agctaaacct
     3181 ttttgaatgg ctaagtccct attaattact tctacagaaa tgttccataa agaaatcaac
     3241 cgcaaagtat ctttcttatt aaattcttgc tttattaatt cattcaattg cccaagataa
     3301 ttaagcggca catctgggat aaccagtaat aatttgattt ctatgctacc ccattgcaaa
     3361 tttgaaaaag cactaggcac aattgtttct ttgatatctt ctctgcctaa tattcccaaa
     3421 atttcaacat ttaatgcatt acaccatttt tgataaatct ctgaaatata ttcatcaaaa
     3481 cgctcaggag atctttcttg atttggaata ctttgttttg cctcaacaaa taacaaatta
     3541 tcttcttgca gatagataaa ctctactgtc ttaatacctt cttgccttct cttccggata
     3601 tatggttcta tataaaacag gttatttttt actagattaa aagttatacc tgattcagtg
     3661 agatttatta tttcactcat aaaaacctaa ccaatccatg ctgtttccgg agataaaaca
     3721 cctatttctt ctctgaacaa tcgaatagac tcaccaataa tttcattatc cggcatgcta
     3781 ttttgtaaat tttcctgcac ccaagtagtc tgttcctttt ctttgccagg cataaaaaca
     3841 ggaatacttt cactatgctt tctagctaat aaacacagct tcttgataac aaaatatgaa
     3901 tgcgtactca taaaaatctg tattcccaat ttggatttag ataaggaaaa cagaatttct
     3961 agaaattgat taattgcttt cggatgtaga ttagcttctg gctcatcaat aaataagatt
     4021 gatccaggac tcaaaaaccg atttcctaat agagtgtcta atatcccaat cttttttacc
     4081 ccctctgctg tagaattgat agagaaagtt tgtctatctt tttcatatac ccatgccttc
     4141 tttttatcat tgtagctaac ataaccgcca aacaatgctt ctagatcctt tctagcttcc
     4201 gctgcctcag ggtaattcct cccttttgtt ggtttaatat ccaatgcttt taccaaatca
     4261 atgtaagtag catcaaaacc aaatctcttt tcttcctctg attttttaat tacatcaaat
     4321 agtgatagaa cttctttggg tggtaaaaaa atagagttag cgtttattct gggagaaagt
     4381 tcgtatagct ccggtttaat tagctttttg gtagttctac caaaagaaaa caaaactgaa
     4441 gaactatctt ctaaagtcat acttaactta agaggccgtt cgtttggatt ttcttttcct
     4501 cttgtaacta aatcaccaat ttcctctact tggaacgtcc aatatagctt atctcgcaat
     4561 tcctcagaaa aatcacgaat attctttcct ttatgagctt cctcatgact ttttaaaaca
     4621 ctatataatg cctttaaaat aaaagttttc cctttgctat tttctgcaat aattaaattt
     4681 attttcccta aattatcagc cttcaataag ctaatcggcc cgaaattttc aagccaaaat
     4741 gatttaatca tataatcccc ctaatatata tcaacaatat ttgtacaaga cacattgtga
     4801 aaagacagca accacagcac catagtaaac gcatagcagg cggataatgg agcgacatag
     4861 cgtttcatga tttagctttc tgaggtaggc acaggctact tttttcattt ttaggcagcc
     4921 tacacaatca ttttacaaac tgcttcaaaa aattcaaatc gttatcaaaa aacaagcgca
     4981 aatcgttcac gttgtaacgc agcatcgcga aacggtcgag accgataccg aaagcgaaac
     5041 cggtgtattt ttcgggatcg atattcacat ttctcaacac gttgggatgc accatgccgc
     5101 agccgccgac ttccagccat ttgccgtttt cgcccataat gtcgatttcg gcagacggtt
     5161 cggtgaacgg gaagaaagac gggcggaaac gtacttgcaa atcatcgcgt tcaaagaagc
     5221 gacggataaa atccgtgaac actgctttca aatcggcaaa agttacgccc tcttctaccc
     5281 acaaaccttc cgcctgatgg aacataggcg agtgcgtggc atcgctgtcc acacggtaaa
     5341 cgcggccggg ggcgataatg cggatgggcg gctctttttt atcgagcata tagcggattt
     5401 gaatcgggga agtgtgcgta cgcaaaacat cgccgttttc aacgtaaaac gtatcctgca
     5461 tcgcacgggc aggatggttt gcagggatgt tcagggcttg gaaattgtga aaatcgtctt
     5521 cgatttcagg cccgtccgcc acttcgaaac ccattccgtg aaagagttcg accacacgtt
     5581 gcaaggtcag ggttacggga tgcaggctgc cgccttcctg agcgcgtccg ggcagggtaa
     5641 tatcgagggc ttcggcggca agtcgggctt gcagcttgac ttcgttgagg gcttcgcgtt
     5701 tggcattaaa agccgtctga aaccggtttt tgcattcatt gatatgcgca cctatggttt
     5761 tgcgctcttc aggcgacatt tgccccaaag ttttcagaag tccggtcaac tcgccggttt
     5821 taccaagata acgggctttg atttgttcta gagcgttgaa gtcttgcgca gcttctactg
     5881 cggcaatgcc ttctgcaacg atgcggttta cattttccat aatatcaagt ctgtcaatta
     5941 atgatatacc acctgaaaca acgaggccgc ctgtatagcc tgacatatca gactcttcag
     6001 acgaccttat tttcttcacg gtcaatccat tggataaatt ttctgtatag tggattaaat
     6061 ttaaaccagt acggcgttgc ctcgccttgc cgtactatct gtactgtctg cggcttcgtc
     6121 gccttgtcct gatttaaatt taatccacta tattttaacg caaatttatt taaaaaaata
     6181 gactttaaag aaagaaaatc aatcacatag aatttctgca aacaaagaaa aaaggaagcc
     6241 gcagcttcct tttcaatttt ttggattaag cagccaaagc agctttggct ttttcaacca
     6301 attgtgcaaa agcggcttta tcgaacacgg ccaaatcagc caatactttg cggtcgattt
     6361 caatagaggc gcgtttcaga ccgttcataa atttgctgta agacaacccg ttttcacgcg
     6421 cacctgcatt gatacggaca atccacaatt gacggaattg gcgtttgcgt tggcggcggt
     6481 cacggtacgc gtattgaccg gctttcatta ccgcctgctt ggcaacgcgg taaacgtttt
     6541 tacgacggcc gcggtagcct ttggctaacg cgaagatttt ttggtgacgg gcacgagcgg
     6601 taacaccgcg ttttacgcgt ggcatattct aaactcctta agcgtagggt aacattttag
     6661 caacagaagc caaatcgcga tcatttacca tagaggtacc gcgcagttgg cgtttgtttt
     6721 tggtggtctt tttagtcaag atgtggcgtt tgaacgcatg agcgcgtttc acaccgccgt
     6781 tacccagtac tttaaagcgt tttttcgcgc tagacttggt tttcatttta ggcatgggaa
     6841 aactccattc gttatcggat aaggcattag ggggttttaa aacatcagtt tcaaacactt
     6901 gaacccacaa taccacggtt ttttgccgca attgaaaact tactccgaag cggcaatcgg
     6961 agtaagcgga gaattatagc tttatttttt cttcggcgca atcatcatca ccatttggcg
     7021 accttccatt ttgggaaagg actcgatttg cgccacttca gccaaatctt cttttacacg
     7081 ttccaaaagt tgcgcgccga gttgctggtg agccatttca cggccgcgga aacgcaatgt
     7141 cactttgact ttatcgccgt cggcaaggaa gcggttaatg ttgcgcatct tgatttgata
     7201 atcgccctca tccgtacccg gacggaattt gatttccttg atttgcacct gcttttggtt
     7261 tttcttggct tcgtcgcgtt tcttggcctg ctggtatttg tatttaccgt aatccatcag
     7321 tttgcacaca ggcggtttag cagttgggga aatctctacc aaatcgacat cctgcccttc
     7381 ggccatagcc aaagcttcac gaactgaaac gacaccaagc tgttcgcctg actcactgat
     7441 taaacgcact tctttggcgg taatttcgcc gttgattcgt gcttcgcgtt cttgagcgat
     7501 gatgaatact cctataaaaa ttaatgattg acgagggcat cagtgatttc ttgctgcaat
     7561 tgcgcaatga aatcatccaa atccaaagaa cccaaatctt ctgctttgcg gcgtaccgcc
     7621 actttgtttt cctgcttctc cttatcgccg acaacgattt gataagggaa acggtattgg
     7681 ctgttgtcgc ggattttgta accgattttt tcgttacgca aatccaactc ggcgcggaat
     7741 cctgccgcct gcaatttggc agccacttcc cgacaataat ctgcctgatt ttcggtaata
     7801 ttcataatta ccaattgaac cggagccaac cataacggga atgagcctgc atggttctca
     7861 atcagaatgc cgataaaccg ctccaaagaa cctaaaatgg cgcgatgcaa cataacagga
     7921 cgcgcacggt cgttgttttc agttacatat tcggcattca aacgctccgg caatacgaaa
     7981 tccagttgta atgtaccgca ttgccaagaa cgacccaagg catctttgac atgatattcg
     8041 attttaggcc cgtaaaacgc accctcgccc ggcaattcgc cccattccac gccgcaggca
     8101 gtcaatgcct cgcgcaaacc ctgctctgcc ttatcccaca cgtcatctga acctgcacgt
     8161 ttttcagggc gaagagaaag cttgacggat acatcatgga aaccgaactg tttgtagatg
     8221 cgaaccaaca attcattgaa cgcacgagcc tcgctgacga tttgatcttc ggtacagaaa
     8281 atatgcgcat catcctgcac aaaaccgcga acccgcatca gaccgtgcag cgcaccgctc
     8341 ggctcattgc ggtggcaaga accgaattcc gccaaacgca tcggcaaatc tcgatacgaa
     8401 cgcaaaccgt tgttaaaaat ttgaacatga cccggacagt tcatcggttt aaccgcatat
     8461 tcgcgttttt ccgaactggt tacgaacata ttgtctttgt agttgtccca atggccggat
     8521 ttttcccaaa aggttttatc catgatttga ggcgttttga cctctttata accggcggca
     8581 ttcagctctt tccgcatatg ctgctcaatc acttgccaca aagcccagcc tttgggatgc
     8641 caaaacacca tacccggtgc ttcgtcttgc aagtggaaca aatccagttg tttgccaagc
     8701 ttgcggtggt cgcgcttttc ggcttcttcg atacgttgaa tataggcttt caactcgtct
     8761 tttgtcgccc aagccgtacc gtagatgcgt tgcagcattt cattgttgct gtcgccgcgc
     8821 cagtatgcgc ccgccaactt ggtcagtttg aagtttttca ggaaacgggt attcggaacg
     8881 tgcgggccgc ggcacatatc gacatattcc tggtgatgat acatccccat cgcttccact
     8941 tcgggcatat cgtcaatcag gcgcagtttg tattcttcgc cgcgctcttg aaaaatttta
     9001 atcgcctccg cacgcggagt catgattttg accacatcat agtcttgggc aatcaattct
     9061 ttcatacgcg cttcaatggc ggcaacatct tccggtgtaa acggtttttc cgtggcgatg
     9121 tcgtaataaa agccctcttc aatgacgggg ccgataacca tttttgcatt aggatagagt
     9181 tgcttgacgg catgcccgac aagatgcgcg caggaatggc ggatgatttc gatgccttcc
     9241 tgatctttcg gagtaatgat ttgaacagca gaatcttcaa caatcgggtc gcacgcatcg
     9301 accaatttgc cgtttacctt gcctgccacc gccgccttcg ccaaaccggc accgatagac
     9361 gcagcaattt gagccacggt aacgggggat tcgtattggc ggactgagcc gtccggcaag
     9421 gtaatattca acatcaaacg ctccgaaaga ttaaaaaata acggcataaa tgccgttatg
     9481 gggatttggt aggcacgatt ggattcgaac caacgacccc caccatgtca aggtggtgct
     9541 ctaaccaact gagctacgtg ccttcaaaga attttcgcat tttatcgagt cgttttaatt
     9601 tttgcaagag gtatatgcat ttctttatgc aacattgtgg caagatttgg ggatgtcgtc
     9661 tgaaaaaccg tgggaattca tttataatgc cgtcttttaa aacatatttc gacccctgtc
     9721 gcccaactga cattcaatct tatgcttaaa tttactttac acaaaaaaga cggatacgcc
     9781 cgacgcggca cgctggagtt gaaccacggc aaaattgaaa cgccggtatt tatgcctgtc
     9841 ggtacttacg gttcggttaa ggcgatgaac ccgcaaaacc tgcacgacat caaggcgcaa
     9901 attattttgg gcaacactta tcatttgtgg ctgcgtccgg gcttggaggt cgtcgaacag
     9961 tttggagggc ttcacggctt tatcggctgg gacaaaccga ttttgaccga ctcgggcggt
    10021 tttcaggttt tttctttgtc ggatatgcgc aagctgacgg aagaaggctg tacgttcaaa
    10081 agcccgatca acggcgacaa gctgttttta tcgcctgaaa tttccatgaa aatccaaacg
    10141 gttttgaact cggacatcgc gatgcagctg gacgaatgca cgccgggcga agcaacgcgc
    10201 gaacaggcgc gaaaatcgct gcaaatgagc ctgcgctggg cggaacggag caaaaaagcc
    10261 tttgaagatt tgaaaaaccc gaacgcactg ttcggcatcg tgcaaggcgc gatgtatgaa
    10321 gatttgcgcg aagagtcgct gaaaggcttg gaagagctcg atttccccgg cttggccgtc
    10381 ggcggcttgt ccgttggcga acctaagccc gaaatgtacc gcatgttgcg cgccgtcggc
    10441 cctatcctgc cggaacacaa acctcattat ttaatgggcg tcggtacgcc tgaagactta
    10501 gtgtacggcg tggcacacgg cgtggatatg ttcgactgcg tcatgcctac tcgcaatgcg
    10561 cgtaacggct ggctgtttac ccgttttggc gatttgaaaa tcaaaaacgc caaacacaag
    10621 ctcgacaagc gtccgataga cgaaagctgc acctgctacg cctgccaaaa cttcagccgc
    10681 gcctacctgc accatctgca ccgcgccggc gaaatcttgg gcgcacagtt gaacaccatc
    10741 cacaatctgc atttctacca agtcattatg gcggaaatgc gcgaagccgt cgaacaaggc
    10801 aaatttgccg actggcaagc acaattccac gaaaaccgcg cacgcggcac agactgactc
    10861 cctgcaatgc cgtctgaagc cgcttcagac ggcattttgc cgcgttaccc tacccgcact
    10921 atttttcaga aaccgatcat gctcccattc cttccagaac cctccctttc ctatacgcaa
    10981 caaaaccgta ccgccgtttt gctgttgaac ctcggcacgc ccgacgcgcc gaccgcgcag
    11041 gcggtacgac cttatctgaa gtcctttctg accgaccggc gcgtcgtcga actgcccaaa
    11101 tggctgtggt atcccattct gcatggtctg gtactgacgc tccgccccaa aaaaagcgcg
    11161 cacgcctatg aaaaaatctg gtttaaggaa ggttcgccct tagaagttta cacagcacgc
    11221 caagctgccg cgcttgccaa acgtatgccc gacctcatcg tccgccacgc catgacctac
    11281 ggcaatcctt cggtggcaga cgtattgtcg gaactgaagg cgcaaggcgc gggcagactg
    11341 ctggtcatcc cgatgtaccc gcaatatgcc gcttccagca gcggagcggc agtagataag
    11401 gtgtgcgaac aattactgct gcaacggaac caaatgagcg tccgcaccgt ttcgcgcttt
    11461 tatgacgata ccggctacat tgatgcgatg aaaaaccaca tcctccgata ttgggcggaa
    11521 cacggacgtg ggaaaaaact gatgttgagt tttcacggcg taccgcagaa gcactacgac
    11581 ctcggcgacc cctatcccga cgagtgccgc cacaccgcca aactgcttgc cgaagcactg
    11641 gaactgaccg aagaccaata tgtcgtatcg ttccaaagcc aattcggcag ggcaaaatgg
    11701 gttacaccga gcacgcagga tttgttcggc aaactgccca aacagggggt aaccgagctg
    11761 gacgtattct gcccgggctt tttggcagac tgtttggaaa ccatggaaga aatcgccctg
    11821 atggggcggg aacagtttta tgaagcaggc ggcaaaagct accgctacat cccctgcctc
    11881 aacgacaatc ccgactggat agacgcactc gtcgcacttg ccgaagaaaa ccttggcggc
    11941 tggcgttgaa actgtttgac ccatgtctaa aatgccgtct gaacgccgtg ttcagacggc
    12001 attttcccgc tactttagcg tatgatggca tctatgctgg acaacacatc aagtgcaacc
    12061 ttactaccga ttcggcaaga aaatctttca cccgctacgc aaaatcaaca acaaggtttc
    12121 aaacattaag gaaacaccaa tgaacacaac ccgactgccg accgccctcg tcttgggctg
    12181 cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg acaaaagggc aaaaagtgta
    12241 cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa ggccgcggaa ccatgtttcc
    12301 gccgctctac cgctccgact tcatcatgaa aaaaccgcag gtgctgctgc acagcatggt
    12361 caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc tacaacggat tcatgcccgc
    12421 cactgccatc agcgatgcgg acattgccgc cgtcgccact tatatcatga acgcctttga
    12481 caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag gcaaaaaaca aaaaaaaact
    12541 aaacagacaa aatgccgtct gaaaccggca atccggcttc agacggcatt caaatcaaac
    12601 ctttctaacg cggtactttc agccccatag acccgatatt gccgtccccg tcaacctcct
    12661 tcgcggtcag cataaagccg ccgatgtcaa tacggccgca ctcgaccggc tcctgactgt
    12721 cggaacgctt atcgaaaagc tcggcaaggc tcaaaccctc ttcgcccgct tccagcctca
    12781 aaccgtaagc aagtgccaaa tcacccgaac gtgctgccgg caaaacgaca aactcaccga
    12841 agaaatcaaa atactcacgg acgctaatac ccgtctcggt aaagtatttc gccattttat
    12901 agtgaa
//
LOCUS       DAWFIT010000058        12246 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFIT010000058 DAWFIT010000000
VERSION     DAWFIT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.058"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WGQ85_001468"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WGQ85_001468"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0750868.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WGQ85_001469"
     CDS             complement(1334..3490)
                     /locus_tag="WGQ85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0750869.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGVNIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WGQ85_001470"
     CDS             complement(3583..3789)
                     /locus_tag="WGQ85_001470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0750870.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGQ85_001471"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGQ85_001471"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0750871.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WGQ85_001472"
     CDS             4593..5192
                     /locus_tag="WGQ85_001472"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0750872.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKINIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WGQ85_001473"
     CDS             complement(5238..6026)
                     /locus_tag="WGQ85_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0750873.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDTVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVRALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WGQ85_001474"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WGQ85_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0750874.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WGQ85_001475"
     CDS             7840..8316
                     /locus_tag="WGQ85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0750875.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8921..9307)
                     /locus_tag="WGQ85_001476"
     CDS             complement(8921..9307)
                     /locus_tag="WGQ85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0750876.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9304..9552)
                     /locus_tag="WGQ85_001477"
     CDS             complement(9304..9552)
                     /locus_tag="WGQ85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0750877.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            complement(9922..10245)
                     /locus_tag="WGQ85_001478"
     CDS             complement(9922..10245)
                     /locus_tag="WGQ85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0750878.1"
                     /translation="MITRPAQSDLVRIYNFLNDLGAERQANTVMRLLKNSFVSTQSSP
                     SNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFRENAYHL
                     SFPFL"
     gene            10243..10350
                     /locus_tag="WGQ85_001479"
                     /pseudo
     CDS             10243..10350
                     /locus_tag="WGQ85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10356..10643)
                     /locus_tag="WGQ85_001480"
     CDS             complement(10356..10643)
                     /locus_tag="WGQ85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750879.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVEGLGGDESKDMPECRK"
     gene            10707..10835
                     /locus_tag="WGQ85_001481"
                     /pseudo
     CDS             10707..10835
                     /locus_tag="WGQ85_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(11025..11768)
                     /locus_tag="WGQ85_001482"
     CDS             complement(11025..11768)
                     /locus_tag="WGQ85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0750880.1"
                     /translation="MQADEASGRQDAEEGLQGDDPTAHPPQDQTPQSAADNQPKTGVF
                     GEKYFDGTGEITALVIGGETIDLTKADEQVSRRNHPSLMSDFDKVMVSQNQYKHLIYG
                     AAKLKDGSYTLFVQGKPTEQLPVGNATYNGKVLHFRKAYENNWFNDSAYQAAGTFTAN
                     VDFSQRTVTGKIDTKDPSGMGMQEFTASIDGSGFQGKWKSDNDQGDISGKFYGQDGAE
                     MAGRYDYKNQDNRKDGFGVFAGKKEADAR"
BASE COUNT         3107 a         3279 c         2730 g         3130 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tcctcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggta tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccagacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gaactgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacgcaaaaa ttcaaaaaag ataaaaatgc
      961 caaacggtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtaatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcaatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa agcgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttggatg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcccgac acggtagttc tgcccattca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatac
     1861 cgacgttgta cagcacttcc tcaaatgaag tctgtttgtc gttgagatcg gcaaggtatt
     1921 tttctttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg cctgacacgg cgaaattcaa ccatgcagga ttgggtttgg
     2101 catgttcgga tgtaataatt tcgacggaat cgccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taaggaccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cacacccacc agccgctggc cgaatgcgcg caacactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagtgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcattgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagaccgtc caccatctcg gcaatcgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acacagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caattggatg cgtaatgtat ggttcgccac ttttccgggt ttgtccgtca tgagcgtgaa
     3361 atgcataggc aacggccttc tcgagctcaa cctgttcttc aggcttgaga taggaagcgg
     3421 tacggaaaag caaggtgcgt gcttcggcag ttaaatggtc gtaaggtgcg gtgggttgag
     3481 ggacaggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttgag acgcgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacag cttcaggcag cgcatctcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaatcacgt cataacctgc cgccgccaac gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga atacgtctgc gtattccaaa aaagcctcct
     4261 gtgcgataag cgactcaaac tcttctttgg aaacaaagtg ataatgtacg ccgtttgctt
     4321 cgccttcacg cggcgggcgc gtcgtgtgcg acacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaatggaca cccagtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gtgcggaata cttccgcctt ttggtcgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcaacatcgt tgccggtttg gacgcacgcg gcttcattat cggcgcggca ctcgcctacc
     4861 agctcaatgt cggtttcgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acgatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagccgcc gccattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggat gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc tggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttcagacagc atttccacat cgttcaaacc gtcgccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcacgcacca cgcttctgat gccgtctgtt
     5461 ttcgacattc ccgcaggcag cagatcgacc gcttcctcgt gccagcgcac cgttttcaag
     5521 ccttcccgtt ccacaatatc cgaccagggc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgctgcaagg cgcggcacac gcattccgac agcgcggaca cggcgatccc ctctccgccg
     5701 acaaacgcat aatccatacc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgcg cacagttttt ccgtgcagca gcgcaaactg cccgtttatc
     5821 gttaccacgg tatccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatgcctt tgccgcgcaa agccgccacc
     5941 gccgttttca cggaaggacg caaggtatcc gtatatttgc ggtacagcgt atcgtcaata
     6001 tcgaaaaaca cgattttggg attcatcata ttctctctcg cactcaaact gccgcattgt
     6061 atcccaatca ggcaaatact tgataaatcc ttataaattt tccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcacac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cggaaagggt tacacccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactttt attaccggca ggcggtctcg aacgtatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaat aacgaatttg
     6481 ccaaactcga cgttctcgag caaggcatta aagaagcgca tgatcgcaac aagaaattct
     6541 tccttaccgt caatacgctg ccgcacaact ccaaactcaa aaccttcgtt tccgatatgg
     6601 agcccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggc ttgattatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcatcca cggcgcattg tgcatcgcct actcaggccg ctgcctgttg tcgggctatt
     6901 tcaaccaccg cgaccccaat caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagt gatgcaggcg atgcccagct tctgcaaggt tttaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaagtcttc ctgattgaag agtccaaccg cccgggcgaa atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaattccaa agaccttcgc ggcattgaag
     7201 tggttgaaaa actcgccaaa atcggcgtgg acagtctcaa agtcgaaggc cgcaccaagt
     7261 cgctttatta tgttgcacgt gtcgcccagt cctaccgcaa agcgattgat gatgccgttg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggcctcgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaaa cccaagacta tcaaaactac ctgaccggcc
     7441 actctattgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac gaaaacggct
     7501 gggcgaccat cgaagtcaaa aaccgcttcg ccgtcggcga ttcactcgaa atcatccacc
     7561 cgggcggcaa ccaaaccatc aaattggaac aaatggcccg caaaggccag cctgtcgatg
     7621 tcgcacccgg caacggcatt caggtcaaaa tccccgatat gcagggcaaa gaaaaagccc
     7681 tcatcgcacg cgtattgaac ccctgagcca caatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc acagcaaaca aggacatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgctggcagc ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccgaca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgctgcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accgattgca aacggcagga atctggttac acggcaagca
     8101 gcaaacagcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc cggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcaaccgct gcaaggagga caatacgact gtcaggcagg ctcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcgacca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag tcgcagacag tacagatagt
     8521 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     8581 gaggcaacgc cgtactggtt tttgttaatc cactataaaa cacagccata tatattaagt
     8641 acctgctctt tatgcgcttt tttaggaaag gaagatcaaa cggttcaata gcgaagtctt
     8701 gcccaaattt gatggacagg atatagaata catgcgtgca taccacaccc tgccaaagtt
     8761 gggagatgca cttaaggttg cacttgtggt cgccaactat gcctatacac gcggatttcc
     8821 cgcgtttata ggccgcgacg gaagtctggc ttttaagcga agacaataac cgaaaagacc
     8881 gcctcggcgg tctttcttgt gtacacagtt tttatatttg tcagcttgat gcattgacaa
     8941 ctctaattcc atattgcgga atatattcat cgacagtcat cagttcaaag ccttccgctt
     9001 gggtttgtgc aatcaacatc ctatcgaaag ggtctttgtg tatttccgga aggcttccag
     9061 cctgttttgc atgaaacaga cctataggca gcatttcaaa atcctcttct tgaagcacat
     9121 caaaaaactc ttccggtaat ttcaacaacc ccttgttctg cttgatggaa atttcccaaa
     9181 tacttgctgc actgacaaag atcgcatttc tcggattttc tatcagtttg cgtgcagata
     9241 tccccagttt cttgtcatcc aacaaccacc acagcaaagc atgggtatca agcagaatct
     9301 ttctcacaga gccgactcct caaaaaataa agctgccgtt tcattgtcat cctcaagaat
     9361 acgtgaaata tccgtatttt ccatatgact gaattttttc aaccttcctg catttcgtgc
     9421 cggtttttca ataccggtta gttggacgca aggcttacct gccttcgcaa taataacgat
     9481 ttcccctgct tctgctcttt gaatcaattg actcaaattg gtttttgcct gatgaatatt
     9541 tgcttgaaac ataacacttc tcatgattag ctaacttgac taatatacat cattaccaag
     9601 attttgggaa tctcattaca tatatttgat tatatccgcc gttttattca caccttgcta
     9661 tttagtaaca atcttcggca accaaaaatg ccgtctgaaa catttttcag acggcatttt
     9721 taatcccctt gcctgattgt gcggcagatt caaatcgggg cactccatac ttccatatct
     9781 gcaccgttca aacatcccgc ctgcggaacg gaatcaaagc ggcagtccgg ttatgttctt
     9841 gaaggcggtt tatccgaacg tgccgcaata aagccgaaac gcattgcccc gcacaaggga
     9901 aagccgccaa accaaatgac gttataaaaa aggaaagctc aggtggtagg cattttcacg
     9961 gaaatgcctc accgtcaaaa tcaatatttg cttacctgat ttatcgtgcc aaaacaaata
    10021 gctataaccg cttttgccat agggcacgat tacttccctt acattttgca aagtttcccc
    10081 cgccaccgac aaatcataag gtttcccatt actcgggctg ctttgtgtag aaacaaaaga
    10141 atttttaagc agccgcatca ctgtatttgc ctgacgttcc gcacccaaat cattcaaaaa
    10201 attataaatc ctgaccaaat cagattgagc ggggcgtgta attatagtgg attaaattta
    10261 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg
    10321 ctgtactggt ttaaatttaa tccactatat caacttcact tgcggcattc gggcatatcc
    10381 ttcgattcat cgccacccaa gccctccacc cagtccgcaa actcattaag cgtaatatgc
    10441 aatcctgttt cccgataatc ctgaaaagcc tgcatcgccc gcttatcgta ggcttgtttg
    10501 gctttcaagc gttctatatg accatccacc gcatccaata cgatactgtg cacactgccc
    10561 ccgaaatccc gggccagctc ttgtaaaacg gctttgcggt atcccagttt gacaccggtt
    10621 acttcctgat tggtaagcat cattattttc ccataaatca aacgtctgac acggcattat
    10681 aaacacaatg cggcatctgc cgccaccttt gcggacgcag tgttaccggc ttccagagct
    10741 acttcgacaa gcagccgctg caaggcggac aatacgattg tcaggcaggc tcgttccacg
    10801 tccgcgtggt tatgcgcgcc aatgtcgtcc gttaacatat cggcaaccaa aaaatgccgt
    10861 ctgaaacatt tttcagacgg catagtccat aaatcaaatc agtaatacag atgatggttc
    10921 aattaggatc gcttctgcag gcaggtcaac acatcaaagt gttgaccgat accctgtccg
    10981 aaggaaaccc gaactgttcc cgactgtgaa acataggata aatatcaacg ggcatctgct
    11041 tcttttttgc ctgcaaatac gccgaagccg tcttttctat tatcttgatt tttatagtcg
    11101 tatctgcctg ccatttccgc gccatcttga ccataaaatt tcccggaaat atcgccttga
    11161 tcattatccg atttccactt accttgaaat ccacttccat cgatacttgc agtaaattct
    11221 tgcatcccca tacctgaagg atctttggta tcaatttttc ctgttacggt tctctgtgag
    11281 aaatcaacat ttgccgtaaa cgttcctgca gcttgatatg cgctatcatt aaaccaattg
    11341 ttttcgtatg ctttcctaaa atgcagcacc tttccattgt atgttgcatt acccaccggc
    11401 aattgttctg tcggcttacc ctgtacgaaa agagtatatg agccatcttt taatttagcc
    11461 gccccataaa tcaggtgctt atattggttt tgagaaacca ttaccttatc aaaatcactc
    11521 atcaaactgg ggtgattcct acgtgatact tgctcatcag ctttcgttaa gtcaatggtt
    11581 tccccaccaa tcaccaacgc cgtgatttct cccgtaccgt caaaatattt ctcgccaaac
    11641 acgcctgttt taggttggtt atcggcagca ctttgggggg tttggtcttg gggaggatgg
    11701 gcggtcgggt cgtcgccttg caacccctct tccgcatcct gcctgccact tgcttcatca
    11761 gcctgcatcc cgccttgccc cacctcatca gaattttcgg ctttcgacgg cgggtttgca
    11821 tcttcggcat tcatcgcagg ctcagtatgt tcggttgttt cagaaggagg acttgcctgc
    11881 atccccgtat cttcgcttac agcgttttct tccgtaccat cttgagattc tgaagttgaa
    11941 gaaggttgtg ttggatcagt ttctttagct tcttttcctt ctgatgtatc ttctttaggt
    12001 gccggtttga tttcttctcc tgcttctttt ggtgttgact ctgattccac tgttccttca
    12061 ggtgttgatt ctgttgaagt ttccacatgt tggggcgcat cattttgcac tccgccatca
    12121 gcgcctgcct gcgcggtttc cccttctccc tgcgtatctt taggcggtat ttctgttttg
    12181 gattctcttc catccccgcc cgaagcagtg cctgcagtat cagcacccgg ggcagggctt
    12241 accgta
//
LOCUS       DAWFIT010000059        12200 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFIT010000059 DAWFIT010000000
VERSION     DAWFIT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.059"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(337..975)
                     /locus_tag="WGQ85_001483"
     CDS             complement(337..975)
                     /locus_tag="WGQ85_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0750881.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(972..1343)
                     /locus_tag="WGQ85_001484"
     CDS             complement(972..1343)
                     /locus_tag="WGQ85_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0750882.1"
                     /translation="MKLLFIPLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(1612..2034)
                     /gene="queD"
                     /locus_tag="WGQ85_001485"
     CDS             complement(1612..2034)
                     /gene="queD"
                     /locus_tag="WGQ85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0750883.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(2041..2559)
                     /locus_tag="WGQ85_001486"
     CDS             complement(2041..2559)
                     /locus_tag="WGQ85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0750884.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(2587..3246)
                     /gene="queC"
                     /locus_tag="WGQ85_001487"
     CDS             complement(2587..3246)
                     /gene="queC"
                     /locus_tag="WGQ85_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0750885.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            complement(3431..3625)
                     /locus_tag="WGQ85_001488"
                     /pseudo
     CDS             complement(3431..3625)
                     /locus_tag="WGQ85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            3790..5016
                     /locus_tag="WGQ85_001489"
     CDS             3790..5016
                     /locus_tag="WGQ85_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0750886.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(5037..5318)
                     /locus_tag="WGQ85_001490"
     CDS             complement(5037..5318)
                     /locus_tag="WGQ85_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0750887.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(5397..5531)
                     /locus_tag="WGQ85_001491"
                     /pseudo
     CDS             complement(5397..5531)
                     /locus_tag="WGQ85_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5530..5988)
                     /locus_tag="WGQ85_001492"
     CDS             complement(5530..5988)
                     /locus_tag="WGQ85_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750888.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(6552..6755)
                     /locus_tag="WGQ85_001493"
     CDS             complement(6552..6755)
                     /locus_tag="WGQ85_001493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750889.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSKPKEGAK"
     gene            complement(7319..8254)
                     /locus_tag="WGQ85_001494"
     CDS             complement(7319..8254)
                     /locus_tag="WGQ85_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0750890.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEISPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(8257..8877)
                     /locus_tag="WGQ85_001495"
     CDS             complement(8257..8877)
                     /locus_tag="WGQ85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750891.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIRQVLDQLPD
                     GSPAKAAVFKANKNGTLKTAIAGVDRQTGKAVILPVKVPSKTNIRR"
     gene            complement(8971..9333)
                     /locus_tag="WGQ85_001496"
                     /pseudo
     CDS             complement(8971..9333)
                     /locus_tag="WGQ85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(9429..9701)
                     /locus_tag="WGQ85_001497"
     CDS             complement(9429..9701)
                     /locus_tag="WGQ85_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750892.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(9889..10377)
                     /locus_tag="WGQ85_001498"
     CDS             complement(9889..10377)
                     /locus_tag="WGQ85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750893.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVNFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKQDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(10374..>12200)
                     /locus_tag="WGQ85_001499"
     CDS             complement(10374..>12200)
                     /locus_tag="WGQ85_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0750894.1"
                     /translation="IKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVL
                     SAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEG
                     MTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATT
                     GVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAA
                     VTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNL
                     GDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAA
                     VGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVEN
                     NSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRH
                     DVQTYRQYYNQLRESRGFAVEPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGY
                     FRVDSYDPATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGTVISNVPSTPTTIRG
                     RKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT         2926 a         2859 c         2855 g         3560 t
ORIGIN      
        1 gttttaggca attgaaaatt ttttggcaca tttttatgcg tcaaatttcg ttaacagact
       61 atttttgcaa aggtctcaga atgctgtttc agacggcatt tcgttttccc taaccctgcc
      121 atttgcgcgc actggcacag agtatcggca aggttcaaaa gaccatcgga atttaggaag
      181 ccgggcttgt acccaacggc ggcatcttat tcagtaaggc tccgcctcaa tcaggcagcg
      241 gaaatgtggg cagtttcacg cggaagcggc acacttttcc catatgtcaa acatcggtaa
      301 aaccgtacgc cgtccctttg agttacactg cttaaactac tctattcccg cccatttgtg
      361 cgtctgcacg ctcaactgcc aatgcaccgg cgcgtcgggg cgactgttta aaataccgat
      421 ttggcggatg gtgtcgtaga tgttcatcgc accgtcttgt tcgcagggcg aaaggtagta
      481 atgatgcgcg cggattttgc gttccatatt ttcacagaac gcaacgacat cgccgtcggc
      541 aacaatccgc acttcgtcgg ctgtttcgat acagcttttt tcgtatttgg cggcatagca
      601 ggctttgggg ctggtggcga catagtcgat ttgcggcggc gcgggattga gtccgttggt
      661 ttcgatacag aggaaataac cttccgcctt gagcatgtcc agcagcatat cgagatgcgg
      721 ctggatggtc ggttcgccgc cggtgatgat gatgttgcgg gcggcatagg ctttcagacg
      781 gcctaagatg tcggacaggc tcatcatgcc gaatgtcaaa taatcggtat cacaccagcc
      841 gcaggcgagg ttgcatttgc ccaagcggac gaaaacggcg ggcatgcccg tgttccagcc
      901 ttcgccttgc aggctttcaa agatttcgac gatgcggtat tggggatttt cgggggcgac
      961 gctgattttt ttcataccgc caacaccgcc gccgctgcca aaaatgcggg caaaccttgt
     1021 ttgacgagta tgcctttgtt gccggaagag aacgcgcccc acgcggcggc aatcaggacg
     1081 aagccgagaa acagtaccgt tgcgccgtac accgcgttat ccggagcggc aaaccgcgac
     1141 cacaccagcc cgatgccgag aaagccgtta tacagccctt ggttgccgaa cagggtctgc
     1201 acgcgatttt gttccataaa ttcataaggc agcttgaacg tttccgccgc tttttcgctg
     1261 ggaatctgcg tcatttcaag ccaggcgatg tagaaatgtt cgacggcgac gaagaggact
     1321 agggggatga agaggagttt catggatatt ccttttaatg ggtgtatttg atatggaagg
     1381 tcgtctgaaa aaacggggaa tccgtacagg agaaaaaacc gttttacggt aaattcaaat
     1441 aaggacgctc taaaccgtcc tttccgcgca ggcgggaatc cagagatttg gaattgctgc
     1501 aatttttaaa tattcctgat aaatcaaggt ctggattccc gcctgcgcgg gaatgacgcg
     1561 gttgggcatg tcttattgga atttactata tcgatacgtt caagatattc cctactcccc
     1621 ttcatactcc gcacacgatg tcggcgtttc ccacaatttc acgcggcaca cgttcagccc
     1681 cgcgtttttc agacggtcgt acatttcgac cgccatattt tcggcagtgg tgcggcaggg
     1741 caggcgcagg gttttcatgt tccagccctc caaaagcgcg gcgatttggc attcgcggct
     1801 gttgccgccg tggtagatga aggcgtggtc gaaggggtcg gtaatgtgtt gtttgacaat
     1861 ggctttcaag tcggtaaagt ccatcaccat accgtctttc gcgccgcctt tgataatgcc
     1921 gtctgaaacg gtgatttcga gtttgtaggt atgcccgtgc aggttttggc atttgccgtc
     1981 atgcccgtcg agcatatgcg aggagtcgaa ggtgaagatt ttggtgattt tcatgggttc
     2041 tcaaatgggc aaatagccct gaaagccgat ttcggcgggt ttgccgtgcg gattcggggt
     2101 caggcggata tggaaattgc cgatgcggtc gagcgcaagc aggttgtcca cgtcttttaa
     2161 gttgtcttta tgctgctgaa catagctgtc ggtcaacagg gtttgcaggg ctttttgttt
     2221 ggcttcggca ggcgtggcgg cggcgaacaa cccgaaagcg tgcgcttcgg cgagctgccc
     2281 tgcgcgatag ccgccgacat tggcaaaata caggcgcggg gcgttttcag acggcatttt
     2341 cgttttgggc gtttcggata cggcgatgtc gtaaccgtcc gcccattcga caacctgcca
     2401 gccgtcgatg tggattttgt ccgcatcgcc gaaccacgcg gctttgagtg cggggacggc
     2461 ttcacggtag tcgtcgcaca cggcaaattg gatgtcgtgc acttcgatat tcgacctgcc
     2521 ggcattgccg ccgaggtaaa acatatggag cttgggcata tcgtattcct tatggtttgc
     2581 gcgtgttcag acggcctttt tactttccag atattccgcc agcccgcgtt cgcgcaagat
     2641 acagctcggg cattcgcggc agccgccgac gatgccgtta tagcaggtgt gggtttgctc
     2701 gcggatatag tccagcgcgc ccatttcgtc cgccaacgcc caagtttgcg ccttggtcag
     2761 atacatcagc ggcgtgtgga tttgaaaatc atagtccatc gccagattaa gggtaacgtt
     2821 catggatttg acaaacacgt cacggcagtc gggatagccg gagaagtcgg tttcgcacac
     2881 gcccgcgatg atgtgccgta tcccctgccc tttggcgtaa atcgcggcat agagcaggaa
     2941 aagcgcgttg cggccgtcta caaaggtatt cggaacgccg ttttcggcag tttcgatggc
     3001 ggcggtgtcg tccatcaggg cattgtgcgt aatctgccgc atcaggctca agtcgagtac
     3061 ggtttgtttg acgcccaaat cctgcgcaat ccagcgggca cgttccagct cgacggcatg
     3121 gcgttgcccg tattggaaag taatggcttg gacgttttcg cgcccgtagg tttggattgc
     3181 ctgaatcagg caggtggtcg aatcctgacc gcccgaaaag atgaccaagg ctttttggtt
     3241 tgacatatcg aatcctagtt ttggtgacgc gggaggtttt cgaaccgcga agttggacag
     3301 taaagacgcg gattatacag gcaaacgcgt tcagacggca tctgaacgcc atgccgtctg
     3361 aacacgcggc ttatgccgac ttgatggcga gttccgccaa gcgtttgctt tgtgcgaagg
     3421 cgatattgtt ttcttcggcg gtcgggacgg ggccgctgtc gggaatatct ttttcgacgg
     3481 cttcgcagac ggcggacact ttggggacgg tgcgcaatac ggcttcgcaa ccttcaacgc
     3541 tgtcgatgcc gcgagcgatt cggcgggcgg gattgcgggt gctgccgttt tggaaatagt
     3601 agaggacgag gattttgagg gggatttggg ttcatgggcg gattccgtta aaatggttcg
     3661 gtcttcagac ggcataacag ttgttccgat gccgtctgaa acagccgcag ttcaggcatt
     3721 tgttcctgac ggcaaaattg gctataacat cgggcatcaa tgcccaactt actgaccggg
     3781 caagaaatta tgaccttttt acaacgtttg caaggtttgg cagacaataa aatctgtgcg
     3841 tttgcatggt tcgtcgtccg ccgctttgat gaagaacgcg taccgcaggc ggcggcaagc
     3901 atgacgttta cgacactgct ggcactcgtc cccgtgctga ccgtgatggt ggcggtcgct
     3961 tcgattttcc ccgtgttcga ccgctggtcg gattcgttcg tctccttcgt caaccaaacc
     4021 attgtgccgc agggcgcgga catggtgttc gactatatca atgcgttccg cgagcaggcg
     4081 aaccggctga cggcaatcgg cagcgtgatg ctggtcgtta cctcgctgat gctgattcgg
     4141 acgatagaca atacgttcaa ccgcatctgg cgggtcaatt cccagcgtcc gtggatgatg
     4201 cagtttctcg tctattgggc tttactgacg ttcgggccgc tgtctttggg cgtgggcatt
     4261 tcctttatgg tcggctcggt acaggatgcc gcgcttgcct caggtgcgcc gcagtggtcg
     4321 ggcgcgttgc gaacggcggc gacgctgacc ttcatgacgc ttttgctgtg ggggctgtac
     4381 cgcttcgtgc caaaccgctt cgttcccgcg cggcaggcgt ttgtcggggc tttggcaaca
     4441 gcgttctgtc tggaaaccgc gcgttccctc tttacttggt atatgggcaa tttcgacggc
     4501 taccgctcga tttacggcgc gtttgccgcc gtgccgtttt ttctgttgtg gctgaacctg
     4561 ttgtggacgc tggtcttggg cggcgcggtg ctgacttctt cactctccta ctggcaggga
     4621 gaagcgttcc gcaggggctt cgactcgcgc ggacggtttg acgacgtgtt gaaaatcctg
     4681 ctgcttctgg atgcggcgca aaaagaaggc aaagccttgc ctgttcagga gttcagacgg
     4741 catatcaata tgggctacga cgagttgggc gagcttttgg aaaagctggc gcggcacggc
     4801 tacatctatt ccggcagaca gggttgggtg ttgaaaacgg gggcggattc gattgagttg
     4861 aacgaactct tcaagctctt cgtttaccgt ccgttgcctg tggaaaggga tcatgtgaac
     4921 caagctgtcg atgcggtaat gatgccgtgt ttgcagactt tgaacatgac gctggcagag
     4981 tttgacgctc aggcgaaaaa acagcagcaa tcttgagtat ttttgaaact gtatttttat
     5041 cctaaagaca ttttcctatc tgctgattcc accgtttcct ttctgtgtgc cgccataatt
     5101 tttatgatag acaagccatt caagtttgca ttgactgctt tttcattggc aatatccaaa
     5161 tggctggtcg cttcatctaa aaataggatt ttcggttcgc aatataaggc ccgcgccaat
     5221 acgatgcgtt gtttttgtcc gcctgacagt gcgctgccca tatcgccgat taaggtttca
     5281 tagcccatag gcatggcgga gatttcttcg tgaatcatag caaggcaggc gcaatgttcg
     5341 atttttcctc tatccggttc tgtatcgaaa aaacaagggg ctgtactaga ttagccctaa
     5401 atcccacacc aatcccgcag gattttaagc tgttgagacg gtgtgccgaa gttaaatcga
     5461 aattcgcatt ctttcaagaa cagcgggaaa gatttgcaat cgattccgtt gtattttcgc
     5521 aagacacgtt tagtctagag tctgtatatt acatttattt ttagggtctg ctagccaatt
     5581 tcttgttccc ttcattattt tatcttctga aagaaaatta tttttttcca tgctattaat
     5641 attaatgata tgatttttat ttaaaataat ttttccatat aaaatttccg gtttgtaata
     5701 gcaagagtct gaagcaaatc cttgcccgtc aatataagaa aaataaatgt tatcatcctc
     5761 cttataatat tctaaaacca atccggatat tattttatca aaattccctt catttccatc
     5821 tttcatgact aaaatataat tagcaggcca acctttatcg taaacatatt caaactgata
     5881 gttcccgaaa ctctcgatat ccgaactaaa aaagaagaaa agcaaattaa tagccaatac
     5941 aaaaacaaat aagaacaaaa atagcaaaat tttcaactta gttaacaata tttacctctc
     6001 ctttaaattc aatcctgaaa ggtacccctt accctgggct accaattata gtttccatat
     6061 ttctaaatat tgtttttaca ttactttttt ctccccccaa aggaatgcat tttaaaatca
     6121 tgcttttcag gtgctaatcg atacttacca ttaacatctt taatcacaga tatatttcca
     6181 tgtatagccc aacgtgaaaa atctgagtat tatatacagt tataccttac tgaaacaaag
     6241 ctagctgtac atatctgtac catattttta ctaaaaacaa aaaatattta taattattaa
     6301 ggataagata aaaaccccca acgccagatt aattcttaat aatttcaaat aaaaaatatt
     6361 tgagattttt tattttccaa ataatatatt gaaatattta aagttaaggt agcttgattt
     6421 aattagaaaa tcaaaaaaac cattaattaa tttttcttct ttaaatattt tatatgatat
     6481 tataaaatta aataacattg cgaaaacata tgaaatcatc aaaataccaa atatatagtg
     6541 ggaatgcatt attatttagc tccttctttt ggctttgaca aattgacagt ccattgatat
     6601 ccaatgcttg catttgcaat tgctgacgca cctgcccctg ttttccctgt tagtatcaat
     6661 ggttcttttc ttgcaagatg tgcacctgca caaccaccta atactaaacc accacctatt
     6721 gtaaatcctt cctgttgtat ctctaatgat aacattttct tctcgtgccg cccttaatac
     6781 agattgtgga attggattga cctgagcagg aacttctaaa ataagttttc ctgtaagttt
     6841 tcttcctctt agcggagcag gtgtacttgg aacattggaa atgacaaagt gagtgcgcaa
     6901 agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct aaatgcgccc
     6961 gctgccgcct aaaaggcagc ccggatgcct gattatcggg tgtccgagga ggattaaggg
     7021 ggtatttggg taaaattagg aggtatttgg tacgaaaaca gccgaaatcc tgtgtttggg
     7081 tttcggatgt cggggaaggg cttttttgca aaggtctcaa ctcatgttat tgcaaagatc
     7141 tcatgccttg ttttgccagc aggttgagtt cttctccggc aatcagcgtg gtaaagcccc
     7201 gccacaacac agatctatgt actgtggaag ggcttcaatt tatagctttc gaaaatcttt
     7261 cagctttaag acggcctaaa gttgtaatct ataaaacagg agctcagagt tttacggctc
     7321 actctcgctt cgcgttacca aataccagct tatcggtttt gaacgtatag taatctatcc
     7381 aatcctgcag atattcatac gggtaagaca agtcatactg tttcataaaa tcgaagatgt
     7441 cttgatattc gtctgccggc agcggatcgt aaacaatcaa atccctcggt gagatttctt
     7501 gatctatgcc caaatcgaaa ccgtgcatgg atgcgatttt ggcgtaggta acgatttgcg
     7561 gctgcaggta gaaatcgaaa taggacaatg tttctttggc agccatacgc gcggtttttt
     7621 tatcgaaaag cggctctaag gcggctttca ttgcctcggc atcctgttcg gcgaaagcga
     7681 ggaagaagcg gtaatcgtac aaccgctttt gcagccattt gctaggggtg ggatgcgcca
     7741 agactttctc gctacgttgt ttcaaccgat caagctgctt accctccacc atcagcagag
     7801 tattgtaaat catatgccgg ttgaggtcgt agcggtttat aaaggcttct gtatcgttgg
     7861 cgatgttgtc gatattgcgc accagaaact cacgcagctg tgggctgtcg ctcatcagca
     7921 tcagaaacat cggattttga atgttgagca tgtcacaggg aaagaagaag ggttcagggt
     7981 ctgtagaatt tacactcagc aaaaccccca gctttcctgc aacataagca tattgcttga
     8041 atttcttaag atcatgttca aatagatagg catgggaagc tgcagctcta acgtccatga
     8101 aaatatgttg catagctgca agcggaacgc cttttctttc atctacataa tctatagagt
     8161 caaggcaacc gctattgaaa ttagcagtat tgcctatgat tacattagta atatgctcat
     8221 accatttttg ggtggtcatc atattgtgcc ccattgttat ctccttatat tggttttaga
     8281 aggaactttg acaggaagaa taacggcctt acctgtttga cgatcaacgc ctgctattgc
     8341 tgtttttaat gtgccgttct tatttgcttt gaagacagca gctttagcgg gactaccatc
     8401 gggtaattga tctaaaactt gtctaatcca atcctcactc atttgcgtat atccacccgc
     8461 accattcgga ttcagctgta ccgtaccgtt cctaatctgc ttactttcta caatcaaaac
     8521 gacactacca tcggcagcct gccatacatg atcaaaaccg ttatttccgc cgtatttgcc
     8581 gcccgaaagc actctgaagc cattttgttt agctaaagag tcaaataact gctcggtcat
     8641 tttacctgtc gtgtcccttc cttcgccaat agctttcagc aattgtttgt cagttgctgc
     8701 agtaaccctt aattcagttt gaccgatttt gattttggtt ttgctgacat agccctgtgc
     8761 agtggcaacg gttgctgcct tgtccaaggc ttttttcatg ccttgtaaat tttttgccgc
     8821 tttcgctact ttataggcct gtatcgattc accgataccc ggaaccacac ccagcaaagc
     8881 aaacaggtga tcggcagcgg tctgtgcttg tacaaaactc tgtatatcac cgataattgg
     8941 aataaatcct gtctgaaaaa gcaaaacttc tcgatattct tcaggatttt cacgtatcca
     9001 tttttgttgt tccttttgaa attcatcaca ctttgcacca acgcatctag gatacaaagt
     9061 attattctcc accgctatct gtgccgcatt cgccgctgta ttcacatccc cgcctacaac
     9121 ggcagacgca gtgccggcaa ccagttttga ataggcagta atcttcgctt tagctttttc
     9181 gatttcggtc gcactcatac gactgaaatc agtattctta accaaagcct caccaacaat
     9241 ctcaccgact gcagcgccta tcgcaccatc ctgacacttg cccttattcg ccgccgctgc
     9301 cgcacagccc gctatggcat gggcaatctt gtgggtggta tatcgctgcc agccctctct
     9361 gttgataatc ttgcgtatga ttacgccgtt tgatggctaa tcgtacctac gcaggcttaa
     9421 aatttccatc aatccatatc atttgagttt tcatcttcat aatatggatg atcaaacaaa
     9481 tctccttccc aaatacattc actcgttata ccttgggatt ccatgaaagg aacataagaa
     9541 taaaaatctt tttcatattc tccataaaat atattcatac ctttgagcca tactgcatcg
     9601 cattcatcac acagaataat ttcatcatga agatatttag gtttcgaatg gtagagatag
     9661 ccttgttgac aagcaggaca cattttcatt ctcttattca tgattattcc atataatcaa
     9721 tttatttttg tgggtaagca atagtgattt gattgctgcc tttagtctgt acaacaatac
     9781 gtatatagca atctgataat ttcctggttt ttatcagctt tatcctcaga ttccaaaccc
     9841 aataataaaa gccgtttgaa atttttttca gacggcctta aaacttaatt aaataatgtc
     9901 cggatttaac accaaatcat attcaccaaa ctttggaaat agaattacct tatctttttg
     9961 ggaatataat tctgtttttt catatcgctc attattaata aaatcttgtt tcaaaacata
    10021 gcaatcatcc gtattttcat tgaagaaata agtatatcct tctgctaagt catgagattc
    10081 caaagttata taatctctcc atacagccat agaaagaaaa taataattcg aattggaata
    10141 tttttgaaat tgatgggtta aataatccct tccaaattta tcgaaaaaat ttaccgaaaa
    10201 aaaacctact tcctctcgca aggtaattac atattcaggt tgtgtcgccg aacctattac
    10261 ggcagtatat aactcccctt taagatgttt ttcctcagct tgtttctcag aaaaaggctt
    10321 atgaagtctc ttataaccat aagaccactg ctcacaataa aaaatatctt tcttcatttg
    10381 taaatccttc ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca
    10441 gattgtggaa ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagcttt
    10501 cttcctctta tcgtagtagg tgtacttgga acattggaaa tgacagtacc agggctatat
    10561 tttctaacag cctccttgat ataaccaatc cccgtacttt cttggatttg agaaaattgg
    10621 gtaaattttc ttgaaataat ttcccttgtc gcaggatcat acgaatctac cctaaaatac
    10681 cccgtcgccg atttaggatt ggctacataa agctcattat aagggtattt tgatgacatg
    10741 atacggttaa attcattgcc gttgtttatc ctgattctat aaattggttc aacagcaaag
    10801 cctctggatt cccttaattg attataatat tgcctgtatg tttgtacatc atgtcttgtc
    10861 cacggctctc caggagtcct cagaatagca atcccgttaa atttcggatc cagccttcgg
    10921 atttgattgg taatggcctg gatttcaaga atggcatatt catgttccaa ttcttgtcgc
    10981 ggagtccaac gtatatttac cctcctgcga gctaaaagac tattattctc cactgccaca
    11041 gtagccgcat tcgccgccgt attcacatcc cctttaacca atgccactgc gctgcctgcg
    11101 ataatctgcg agtaggctat gactttttgg cgttcttggg gtgacagttt gcctacatcg
    11161 cgtccgtcca acagggtttc tcccaccatc tcgccgactg ccgcgccgat tgcgccgtcc
    11221 cgacatttgc ctttatttgc taccgccgat gcacagcctg ctacggcatg ggctatcttg
    11281 tgggcaatgt agtcttcgct gagattaaat ttgattttgc tcgctacttc tccgtgtacg
    11341 gtactgacta tcgcacccag tgcggcatcg cccaagttgt ctttcaggct gccgccgttg
    11401 atggcggtat ggacacttgc ggcagcggtg ctgttgatca ggttagcggt cagtttgcct
    11461 gctgcgggac tgtgaaaatg tttgctgacg gcttcggctg cttgggtgtt cagcccgctt
    11521 atgccctgca gtacgcctgc ggttacggcg gcggtggcgg cctgtctgac ggtgctgctt
    11581 ttgcccagtt ctttcagggt atggtttatg tctcctttgt tgttgatgag ggaaactgcg
    11641 gcttggctgg cgagactggc cagtgcggct ttgcctgcgg ctgtggtgat agctgcagct
    11701 gatgtgcctg ctgctactcc ggttgtggct gccgttcctg ctgctactcc agttccggct
    11761 gccgttcctg ctgcggctcc tcccgctcct gcgcctgccg cgcccgccgt tccggcggct
    11821 gccggggcgg acagtgcacc gtaggtcaat acggttacga cgataacgac gacagctgct
    11881 gctgcgggtg tcatgccctc ctgtttgtag tcccatttat cgtaagcaag ctgcacctga
    11941 ttccagttga tgtttttcgc aacttgaagt tgtttcaaat aagcatactc gggctgcttg
    12001 gtgagggttt cgatttgggt tttcagattg cctttcggaa tgtcgacaat gtaaccgccg
    12061 ggtgcggaca gtacgggcgc aacgggaccg gtgaaactcg gcaattgcaa ggtttcgatg
    12121 ttactgcccc gtcctgcctg tttttgccat agagtagatt tgctgctgct cacggtttct
    12181 gtgtggatgc tgcttttgat
//
LOCUS       DAWFIT010000060        11969 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFIT010000060 DAWFIT010000000
VERSION     DAWFIT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.060"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            141..575
                     /locus_tag="WGQ85_001500"
     CDS             141..575
                     /locus_tag="WGQ85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0750895.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WGQ85_001501"
     CDS             complement(624..1967)
                     /locus_tag="WGQ85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0750896.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WGQ85_001502"
     CDS             complement(1972..2625)
                     /locus_tag="WGQ85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0750897.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WGQ85_001503"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WGQ85_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0750898.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WGQ85_001504"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WGQ85_001504"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0750899.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WGQ85_001505"
     CDS             4571..5503
                     /locus_tag="WGQ85_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0750900.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WGQ85_001506"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WGQ85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0750901.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7068..8078
                     /locus_tag="WGQ85_001507"
     CDS             7068..8078
                     /locus_tag="WGQ85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0750902.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGQ85_001508"
     CDS             complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WGQ85_001508"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0750903.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9061..10110
                     /locus_tag="WGQ85_001509"
     CDS             9061..10110
                     /locus_tag="WGQ85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0750904.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPNDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(10179..11720)
                     /locus_tag="WGQ85_001510"
     CDS             complement(10179..11720)
                     /locus_tag="WGQ85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0750905.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3073 a         3622 c         2969 g         2305 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacggc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cgttatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct tccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaactcaa atgcgaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgatgtgaac aaatactgcc gttgagcagc ctcatcgaaa accccatgcc
      601 gtctgaaacg gcacggggtt tgtttatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcacctag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcaccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttggc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg tttggcggcg
     1201 ttgcacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcgcgttcc
     1261 aaatcggcat cgggcatcac gataaaggcg ttgctgccgc cgagttccaa gacggttttc
     1321 ttgaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgtcga gccggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccaaaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagctgga
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttccctgca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatag aaatggcaat cagcaacacc aaaattccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccatcaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 agcctgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg accaggccgg tccgatggcg caaaccgccg
     3781 aagactgcgc gattctgttg aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgaacgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggcgtgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttcag acggcattgc
     3961 aaaatactat caacctcctc aaaacccaag gcgcggaatt ggtcgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agtaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcggc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agttaaacgc cgcatcatga
     4201 tcggcactta tgtactgtca cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcatcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctt atggcggaaa aactgttcgg
     4861 cacagacatt ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgaccttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg tgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt accgacatcg aaagcaaaaa acgctcgatg taccgcaagc gttatgaaat
     5401 gctggacaac atggttgcag agatgaagga cggtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgctccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccggcg tggtggaatg tgctttgccg ggcgtactgc cggtaatgaa
     5701 ccgagaagtc gttgaaaaag ccatcaaatt gggtttggct ttggatgcga aaatcaaccg
     5761 gaaaaacgtg ttcgaccgta aaaactactt ctatcccgac ttaccaaaag gctatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgcgccca tatggaagaa gacgcgggca agtccgtaca
     5941 tgaaggcttg aacggcgcga ccggtatcga tttgaaccgc gccggcacgc ctttattgga
     6001 agtcgtatcc gaacccgaaa tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt
     6061 gcacagcttg gtaacctggc tgaacatttg cgacggcaat atggcggaag gttcgttccg
     6121 cgtcgatgcc aacgtatccg ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcagaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaacgatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagac gcgcacgact accgctactt
     6361 ccctgacccc gatttgctgc ccgtcatcat ttcagacgac cggttgcaaa aagcaaaagc
     6421 agaaatgccc gagctgccaa aagaaatggc ggcgcgtttc gtggcggatt atggcgtgag
     6481 cgactacgac gcgcgcctgc tcactgccag ccgcgtgcag gctgcctatt ttgaagaagc
     6541 cgctaaagag agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cacgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cacgcctcgc
     6661 cgctctggta ggcaaaatcg ccgacggcac attaagcagc aaattggcga aaaaagcctt
     6721 tgaagccatg tgggctgaac ccgaagcctc cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgataccg gcgcgattga agcgatggta gacgaagtgc tggcaaacaa
     6841 tgcaaaagcc gtggaacagt tcaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 gcaagtgatg aaggccagca aaggcaaagc caatcccgcg caggttcaag agctgattaa
     6961 agccaaactt gcctgataag caagatgccg tctgaaaccc aaatccgacg ttcagacggc
     7021 attttcacag tctgaccaca ttaaaacagc ataaaaaaca atattccatg aaccacacca
     7081 ttacactgcc cgaccaaacc acctttgccg ccggcgacgg tgaaaccgtt ttagccgccg
     7141 ccgcccgtca aaacctcaat ctgccccatt cctgcaaaag cggtgtctgc ggacaatgca
     7201 aagccgaact ggtcagcggc gatattcaaa tgggcggaca ctcggaacag gctttatccg
     7261 aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa agcgatatca
     7321 gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcactctg cccgcacgca
     7381 tcgaaaatat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg cccaaagccc
     7441 cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc aacgtcagcc
     7501 gcagctactc catcgccaat ccgcccgacc aagaaggcat tttggaactg cacatccgca
     7561 ggcgtgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa gtcaaagaaa
     7621 aaggcattgt ccgcgttaaa ggcccgctcg gttcatttac cctgcaggaa gacagcggca
     7681 aacccgtcat cctgctggca accggcacag gctacgcccc catccgcagc atcctgctcg
     7741 accttatccg ccaagacagc ggccgcgccg tccatttcta ctggggcgcg cgtcatcaag
     7801 atgatttgta tgccctcgaa gaagcacaag ggttggcatg ccgtctgaaa aacgcctgct
     7861 tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc cacgtacaag
     7921 acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc tgcggctctc
     7981 cggctatgac cgaacaggca aaaaatctgt ttgtgcaaca gcataagctg ccggaaaact
     8041 tgtttttctc cgacgcattc acgccgtccg catcataatt ccccggtata aaaaagattc
     8101 aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgta aaaaaatgcc
     8161 gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca aggcgacagc
     8221 cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg cagcctgctc
     8281 ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc ccaatgcggc
     8341 ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc caccaatacc
     8401 gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg gctctgatac
     8461 gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac acgcccttcc
     8521 acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa tggatgagca
     8581 tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc ttcagaatta
     8641 acttccttca gcagcaccat acggctgttg ggtctgccgc gtccgtcgac cgccgccaca
     8701 ttgaccgccg tcggctcgtt aacctgtgcg cgtaccgcct cgtccaacca ccgctcgaac
     8761 tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct gtaatcttcc
     8821 cgaatattgt gcaaatccat ttaccgctcc tcttgttatt tgaaagattc tacccccgca
     8881 cgcaaaccga tttcaaccgt tgcacaaact ttgtccccaa agccgcagcg acgatttcat
     8941 ccgcaaaacc gccgcatcag gtacaatatc gaaccgtccg actgaggacg gcattttatc
     9001 aacccgtcct gccgcatacg ccgcagaaga accgctttat caagcgagtt aggaaaaatg
     9061 atgtccaaac agcccaccag caaacgccaa tggcgcgacg gcgcagcccc gtctgccaag
     9121 aaaaccgcca aaccgttcaa aagcaaagcc cgtcccaaag acgaaacgga caaaaccgca
     9181 tcccaacctt acggacaaaa agcttcagac ggcatcaaac ctcaaaacct ccccaaacag
     9241 cgcgccgcca aagccaaaaa actcgtcgtc cgcaatccca accaaaaaat tatggaacac
     9301 gcgcgcgatt taaaagaacg ccgcagcgac ctatcgcgca tggaacccga acgcctgcaa
     9361 aaagtgcttg ccgcgtccgg cgtcggctcg cgccgcgaaa tggaagaatg gatcaacaac
     9421 ggctgggtaa cggtcaacgg caaaaccgct caactgggcg acaaagtcac ccccaacgac
     9481 cacgttaccg tcaaaggcag catcatcaaa ctcaaatggg ccgaccgcct gccgcgcatc
     9541 atcctatatt acaaacaaga aggcgaaatc gtttcccgcg acgacccgca aggccgcgtc
     9601 agcatattcg accgcctgcc gcaggccgcc agcagccgct gggtcgccat cggacgcttg
     9661 gacatcaaca ccagcggact tctgattctc accacctccg gcgaactcgt ccaacgtttc
     9721 gcccacccca gcttcgaagt cgaacgcgaa tacgccgtgc gcgtactggg cgggctgacc
     9781 ggcgaacaaa tgcgcgtcct caccgaagaa ggcgtgatgc tcgaagacgg cttggcaaaa
     9841 gtcgaacgca tccgcgaaca aggcggcgaa ggcgcgaaca aatggtacaa cgtcgtgatt
     9901 aaagaaggcc gcaaccgcga agtgcgccgc atttttgaaa gccaaggact caccgtcagc
     9961 cgcctcgtgc gcgtcggctt cggccccatc ggactgccca accgcctcaa acgcgggcag
    10021 ttctacgaac tcaaccccgc cgaagtcgcc aacatcctca aatgggcgga aatgctgctg
    10081 ccgggcgaat accgccgcaa aaaagcctaa acccgccaaa acacaaaaat gccgtctgaa
    10141 acatctgctg tttcagacgg cattttgttc gggcgttttc aggagaaaag gtcgagtgct
    10201 ttgacaaaga tcatcaccac gccgtaggcg agcggtatga cggccaatgc ccaacgccac
    10261 cagaccgata tgccgccgcc ggaaactttt tcgcccacaa ggtaagcgtc ggatatggcg
    10321 gtttcgtcat cggggttgcc gctgtgtgcg gcggttttga tgtctttttc gtggtgtttt
    10381 tcgtgtacgg atttgacggc gaggttgcac aacaaaccga taatcagcag acccgccatg
    10441 atgtacatgg ttacgctgta tgcctgtgcc gccggtatgc cgctgtcgat ttggctttgg
    10501 cgtatgtaat tgaccagtac cgggccgatg acggcggcgg ttgaccaggc cagcaggatg
    10561 cgtccgtgaa tcgcgccgac ctgataggtg ccgaacaggt ctttcaggta ggcgggaatg
    10621 gcggcaaatc cgccgccgta catggaaata atcacgcaaa agccgatgat gaacagggct
    10681 ttgctgccgc cctcgccgat ggagggaacg gcgaaataca gcagcgaacc gagtacgaag
    10741 aagatggtgt aggtgttttt gcgtccgatt ttgtcggaaa cgctcgacca caaaaagcgt
    10801 ccgcccatgt taaacaggct caggaggctg acgaagcctg ccgccgcacc tgcgccgact
    10861 gccgcctgcc tgcctatgga ggtttcggaa aagagttcct gaatcatcac ggatgcctga
    10921 cccaatacgc cgatgccggc agttacgttc aggcacaata cccagaacaa cagccaaaac
    10981 tgcggcgttt tcatggcttg ggacacgttg acatgattgc tgctgaccag cttgttttgc
    11041 gttttcggcg cggtatagcc ttcaggtttc cagccgtcgg caggtacgcg gatggtaaac
    11101 gcgccgaaca tcatcagtgc gaggtaaagc agacccaata cggcgaaggt ttcggcaacc
    11161 ccaaccgaag cagcgtttga aaaggtgttc atcagtgata cggaaagcgg cgaggccagc
    11221 attgcgccgc caccgaaacc cataatcgcc aaaccggtcg ccatacccgg cttgtcggga
    11281 aaccatttca tcagtgtgga aaccggcccg atgtagccca aacccaagcc tacgccgccg
    11341 atgacgccgt tgcccaaata gagcaggaag aggttgtgcg tacgcacgcc gaatgcggat
    11401 acgaagaagc ccaggctgaa acagcaggcg gcggcaaata tggctttgcg cggccctacc
    11461 cgttccatcc acgtaccgaa cagggcggcc gacgcgccca gcatcgcgag tgcgatactg
    11521 aaaatccaac ctacggtcgt cagcttccaa tctccggccg ccgattcggt tatgccgata
    11581 agtttggtca gcggcgcgtt gaatacggaa taggcgtaaa tctgcccgat ggcaaggtgt
    11641 accgccaatg ctgcgggcgg tacgagccaa cggttgaaac ccggcttggc aatgcttgcc
    11701 tcacggtcta aaaacttcat aacatcctct ttctgtcagt tgaaaaataa aatttcattt
    11761 gctcaatgga aacttattga aaattataaa aaaatatcgg gtcgggtttt tatccgcccc
    11821 aagatgcgcc gtctgaaaca tttcgggtgt acggaaaggt ttctgttttt tccgacaaat
    11881 tcctgcggct tttcgcttcc ggattcccgc ttttgcggga atgacgaatt aaagattatc
    11941 ttaaggtcaa gggactggat tcccgcttt
//
LOCUS       DAWFIT010000061        11961 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFIT010000061 DAWFIT010000000
VERSION     DAWFIT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.061"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGQ85_001511"
     CDS             complement(224..1561)
                     /gene="ribB"
                     /locus_tag="WGQ85_001511"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0750906.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1783..2796)
                     /locus_tag="WGQ85_001512"
     CDS             complement(1783..2796)
                     /locus_tag="WGQ85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0750907.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGQ85_001513"
     CDS             complement(2952..3545)
                     /gene="ribA"
                     /locus_tag="WGQ85_001513"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0750908.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3538..4212)
                     /locus_tag="WGQ85_001514"
     CDS             complement(3538..4212)
                     /locus_tag="WGQ85_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750909.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4366..4450
                     /locus_tag="WGQ85_001515"
     tRNA            4366..4450
                     /locus_tag="WGQ85_001515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4400..4402,aa:Leu,seq:tag)
     gene            4971..6005
                     /gene="purM"
                     /locus_tag="WGQ85_001516"
     CDS             4971..6005
                     /gene="purM"
                     /locus_tag="WGQ85_001516"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0750910.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(6479..7135)
                     /locus_tag="WGQ85_001517"
     CDS             complement(6479..7135)
                     /locus_tag="WGQ85_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0750911.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGRSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(7132..8904)
                     /locus_tag="WGQ85_001518"
     CDS             complement(7132..8904)
                     /locus_tag="WGQ85_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0750912.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            9110..9724
                     /locus_tag="WGQ85_001519"
     CDS             9110..9724
                     /locus_tag="WGQ85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0750913.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            9887..10504
                     /locus_tag="WGQ85_001520"
     CDS             9887..10504
                     /locus_tag="WGQ85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0750914.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            10512..11375
                     /locus_tag="WGQ85_001521"
     CDS             10512..11375
                     /locus_tag="WGQ85_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0750915.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(11388..11822)
                     /locus_tag="WGQ85_001522"
     CDS             complement(11388..11822)
                     /locus_tag="WGQ85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0750916.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
BASE COUNT         2891 a         2861 c         3255 g         2954 t
ORIGIN      
        1 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
       61 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      121 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      181 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      241 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      301 ggacgcgcag ttttttgacg ttgaggccga cgaggatttg tgcgccgatg ccgtagcttt
      361 tgctgtccca tttgtaggct tggtttgcac ctttgggcag ggtgcggtcg agcagggatg
      421 cgccgtcttc ggcgcggtgt aagaggatga cgacgccgct ttcggcttgt tggatgcgct
      481 caagggcttt gggcagcgac catgaatgac gcgggttagc ttggatgaag tccatgacgc
      541 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggagggcgtg cctttgacga
      601 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
      661 acggggtttg tacaggcgca ttgcccatgt cttcaagcag gctttcggta cggctgcggt
      721 attcgatgag gtcggcaatc gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
      781 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggaggcag
      841 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
      901 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
      961 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1021 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1081 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1141 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1201 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1261 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1321 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1381 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1441 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1501 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1561 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     1621 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     1681 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     1741 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     1801 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     1861 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgtct tcgcgccgaa
     1921 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     1981 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2041 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2101 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
     2161 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
     2221 caattgcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2281 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2341 ctccaccagc gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2401 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2461 ttgttgtaaa tcaaagagaa tccccccccc cgcgtttttt gaacggttgg tatcggccca
     2521 ataatcgtcg ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt
     2581 gcgggcatcc gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc
     2641 tgcatattcg tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat
     2701 ttcataatcg gcaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc
     2761 cacattgtaa atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga
     2821 tgtttgcgcg tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta
     2881 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
     2941 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
     3001 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
     3061 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
     3121 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
     3181 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
     3241 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
     3301 tctgtctgta cggccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
     3361 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
     3421 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccat ttgcctcttc aaagccgtgc
     3481 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
     3541 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
     3601 agccaaggca agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc
     3661 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat
     3721 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg
     3781 tacatccgag gcaacattca tccagccgct ttgcgccgaa cggcaagcca gcgaaacctt
     3841 gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgcc gggacaggca
     3901 aaccagccga aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac
     3961 gcctttacag cgtgaaaaca ccgaatccaa agccatgaaa agccgtttca gaccttcctc
     4021 attcccgctg ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa
     4081 aaccgaacgc tcaatcgaag tcgtccccat atccattacc gtctgcgccg tcagatacgc
     4141 cgtccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     4201 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     4261 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     4321 accttgcaca aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga cgtggcgaaa
     4381 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     4441 gtccgcacca ataatgataa ataaattata tagttaatgc ttatcgggac aaattagtgc
     4501 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     4561 acacgcagaa cggtacataa agtgccgcca tttgttccgc accgaatcgg aaacgggtgc
     4621 ttccgcccgc aatcagattc actgccccta aatgccgggg tttcaaccgg aaagaagaaa
     4681 atattgattt tgatgatggc atcaaaaaac cccttccgcc cgtctgccaa aaggaatggt
     4741 taagaaaagc aatgcgtctt ttgatggaac gcgatatata aggaattgag gggaagggca
     4801 ggcgggtttc tgcccgttaa tcctgcggac ggtcccgcca aacccgacac gcgcgggcat
     4861 atggcggatt gtttacaata ttgtctaaaa atctgaacct gcaaacgtga aataaggtag
     4921 aatacgcccc gttattttac cgtccaatct ccatttacca aggaaaaacg atgagtactt
     4981 cattgagtta ccgcgatgca ggtgtcgata tcgacgcagg cgaccaactg gtcgaaaaca
     5041 tcaaaccgtt tgccaaacgc acgatgcgcc cggaagtatt gggggatttg ggcggcttcg
     5101 gcgcgttggt cgaaatcggc aagaaatata aaaatcccgt attggtcagc ggtacggacg
     5161 gcgtgggtac caagctcaaa cttgcctttg attgggataa acacgatacg gtgggcatcg
     5221 accttgttgc aatgagtgtc aacgatattt tggttcaagg agcagaacct ctgtttttct
     5281 tggattattt cgcctgcggc aaattggatg ttccgcgcgc gaccgatgtg atcaaaggca
     5341 ttgcacaagg ttgcgaagaa tccggctgcg ccctgattgg cggggaaact gccgaaatgc
     5401 cgggtatgta tcccgtcgga gaatacgatt tggcgggttt tgctgtcggc gtggtggaaa
     5461 aagagaatgt cattaccggc cgcagcatcg gcgcgggcga tgtggtattg ggtttggctt
     5521 ccaacggcgc acattcaaac ggctattccc ttatccgtaa aatcatcgaa cgcgacaatc
     5581 ccgatttgga tgccgagttt gataatggca aaaccttgcg tgaggctgtt attgcgccga
     5641 cccgtctgta tgtgaaaccg attcttgccg ctttggaaaa atttaccatt aaaggtatgg
     5701 cacacattac cggcggcggc attaccgaaa acgtgccgcg cgtgttgcct gaaaacacgg
     5761 ttgcacaaat cgatgctaaa tcgtgggaat tgcccaagct cttccaatgg cttcaaaagg
     5821 cgggcaatgt ggaaacccaa gaaatgtacc ggacctttaa ctgcggcatc ggcatggtcg
     5881 ttattgttgc tgccgaagat gccgatgcgg ttcagggtct cttgggtgaa caaggtgaaa
     5941 cagtttaccg tttaggtttg atccgtgagc gtcagggaga cgagcatcaa acccaggttg
     6001 cctgattgct tctatagtga aatgccgtct caaacatggg tttggatggc atttttatag
     6061 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
     6121 ggcgaggcaa cgccgtactg gtttttgtta atccactata tattgattga tatgagggtg
     6181 ggttacacat gcttaaacga tataagccat tgtatattta tgggattcgg aatggaacat
     6241 tttaccctgc attagtattt ccgcttttta ttatgccgtc tgaaaatatc atgatgatgc
     6301 gttttcagac ggcatagctg atattgcagt atcagatagg ggcaaacgga aaacactaaa
     6361 ccgaacaata ttactgctat gccaagcgta cttatggggg cataagctta catgcttggt
     6421 ctgttatccg aatgaccgta cggggattaa accccctaat atgccgtctg aacccgcctt
     6481 attccagcac gggttggggg acgttgtgcc ggatggcgta aacagcggcc tgcacccggc
     6541 tgctgaggtt gagtttgcgg agcaggtttt gaacgtggac tttgacggtg gattcggcaa
     6601 gatcgaggtg gcgggcgatg attttgttgc tgcgtcctgc ggcgagatag cccaagattt
     6661 ccagttcgcg aggggtaagt gaggagagtg cctgtgtccc ttgggcaggt tggggggaaa
     6721 tcaggctttt aacgagtttg gcggtcatct cgggcgagaa tacattatcg ccttcggcgg
     6781 ctttgcgtat gctttcgagc agaaagtcgg cgttgatgtt tttcagcagg tagccgcgcg
     6841 cgccgatgcg catacattcg gtcaagtcgt cgttgtcttc ggaaacggtc agcataatca
     6901 ctgcctgctg cggattgatg ctgatgattt gggagagtgc ttcgcgtccg ttcataccgg
     6961 gcatatcaag gtcaagcagg acgacatcgg gttggagccg gttgatcatt ttaatgcccg
     7021 agaggccgtc tgcggcttcg ccgatgacct caaaatcatg ttggcgcgac aaaagggatt
     7081 taatgccgct gcggaagagg gtatggtcgt ctatcaggat aattttaatg gtcatttcaa
     7141 gctttcttca gatacacccg ccaatgagac ggtggttccc tgttggggtt gggaatagat
     7201 tttcaatacg gcgcggatac gtttggcccg ctcctgcatg atgtgcagtc cgacatggct
     7261 gcccgtgggt tctcctattt tctccgtgtc gaaaccttgt ccgttgtctt ggatggtcat
     7321 ggtaaagcgt ccgccgtgtt cggaaagggt gaattttaca tgggtggcgc gggcgtgttt
     7381 gcggatgttg gacaggcttt cctgcaggat aaaaatcatt tggagctgcg cttcctgagg
     7441 cggcaggaac gaaccgtttt cccaggcggt ttcgaccgtt atcccggttt gttgcgtaaa
     7501 gcgggcgaat aggtcggcaa cggcttcggg aaattcttta ttgctgattt tggtacggaa
     7561 gttgagcagc agttcgcgga catcttcata acattcctgc acgcctgttt tgatgaagcc
     7621 gatgttttct gcggcttcct cccgtttgtt ttcggcaaag gcggtttcca gcatctgtac
     7681 ctgtaggttt aggaacgtta atgcttgtgc gatgctgtca tgtaatcctt gcgcaatcag
     7741 gttgcgttcc tgcaatactg caagcaggcg tttttcttcc tcctgttttg tgccggcaag
     7801 cgatacgccc aattgcctgc ctagtgtttg aagcaggatg cggtcgtctt catcaagaga
     7861 aatgccgttt ggaaagctga gcaacagcct gcccaatgtt tcgttctggt actcaatggg
     7921 gaagatttcc tcatggtact tccccaaatc cgaagctgct gtgccgcaat ccgcatgatg
     7981 aatggaaaca taaacatcgg attcgccgtc caaacaaact ctgccggaat ctgctcctac
     8041 ggcgggcagg atacggttta gaaaatgttc tgcagcctgt tgcggtatgt aggattggtg
     8101 caggtcccgt gtagtttggt acagcagggt caggttttga ttttgttttt cgagactgcg
     8161 tgtctgctcg gcgacttgtc cttccaaatc atcatataaa attttcaacc tgccgcccat
     8221 ttgattgaaa caacgcccga cctgtttgaa ttccggcgta ccgccttcgg gaaccggaat
     8281 atcgaaacac ctccgtccga tgcgttccgc accttccctt aacgcctgca gcggccggat
     8341 aacccaaatc tggtgccaaa acagcatcag tacagacgac accagcgtca tcaacataat
     8401 tgcccattga aaacgcctga gccaccatgt gtttttttcg ttggcatttt ccaatgcctg
     8461 caaaaacagt tcgatgtttc cggcaaagcg gtagagatcg acctgagtcg gtcgccggta
     8521 ggactggagc ggggggagga tgtgtgcctg ccaatctata atcagcatgg attgtatcaa
     8581 atcataagca agaggggtgt ccgaaggaat cagcggatgg atggcatcac tttgtgaaat
     8641 acgattcagg cttttttcaa attcgtcaac ctgattgtct atttgggagc ggggagatcc
     8701 ttcacccgcc atgtatgcca gacggtatgc ctgcattctc aagttgcccg cctcttcgat
     8761 gacggaggcc gcgttttcca gacgcaaaga gagcagcagt gtcaaaacga cagacaatgc
     8821 cgccaacccg acccacactc cggtcaggag tttcaggcgt agggaaaggc tgatgccgtc
     8881 tgaaaaacgg gctggcagta tcatgctcgg cgaaaaattg ttccaaataa tgcaaacaat
     8941 atcattcttt ggaattagat acaactgctc agaaagaatt ggttaaggaa aacttaatcc
     9001 gcaccgcttc agtgttaaat catgttatga taaaattcag ataatatgaa tttatttgcc
     9061 gtctgcttca gacggcattt tgagtcattg ggaacggcag actgcaatca tgaaaacctc
     9121 gaacttttcc aagagaacat ccgcaatgaa aacttttgcc ctgatactgg ccgacggtca
     9181 ggcgagccgt atgggaggcg aggacaaggg gcttgctctt ttggggggta aggcactgat
     9241 agatcatgtc atcgacaggg tcaggccgca ggtcagccat atcgccatca gcaccaaccg
     9301 gaatttggaa gaatatgctc gaagaagtcc gcatattttt ccggacgcac ggcagtggca
     9361 gcatttcggc ccgctttcgg cattgtgtac cgcagccaac gatttgcagt tggcgactgc
     9421 cgactggctt ttggttgtgc cgtgcgatat gccgtatctg ccggacgatt tggtggcgag
     9481 gtttgaaacc gtgtcgaaac gcacaccgtt gtgcaatgcg tattatgtgg aaacgccgat
     9541 aacgatgcac tacaacatta tgtatatccg cccgcagatt ctgcaaagcg cgattcccta
     9601 tctgttttcg ggtatgaaaa cattaagaag ctggttgcag cagcaaaggg cgcgaccggt
     9661 cagattcgag tttgacgggc attttgccga cttgaacacg caaactgatt tgcaggaggg
     9721 ataagggtaa gaccgccgac cggcgtggaa aggaaaggtc aagccgtacc gggcggtttt
     9781 tgcccgaatc ggaggcataa tgctgtctga aggcatttca gacggcattt ttcgcgggga
     9841 gatgctacaa tttgcaccat ttttaccgac acagggaaac aggattatgt ttacaggcat
     9901 tgttcaagga ttgggaaaac tgacggcaat ccaccgcccg tcggaggcat ttcagactta
     9961 tgtcgtcgag cttccgcaag aggcggcgga caatctgcaa cgcggcgcat cggtcgccaa
    10021 caacggctgc tgcctgacga ttaccgaaat cgaaggaaac cgcgtcagtt tcgatttaat
    10081 ggcggaaact ttggcaaaaa ccaatttagg gctgctgaag gaaggcgatt gcgtcaacat
    10141 cgaacgggcg gcgcgtttcg gagacgaaat cggcggacac gtcatgagcg gacacattat
    10201 ggcaaccgtg cctattgtcg aaatcgaacg ggacgggtac aaccgcacgg tttggttttc
    10261 gctcccgcac gaactcaaac cctatatcct gaccaaaggg ttcgtcggct tggacggttg
    10321 cagcctgacc ataggcaaag tcgaagacag ccgtttcaat gtccatttga ttcccgaaac
    10381 tttggaacgg acgctgttcg gcagcagaaa ggtcggcgac agaatcaaca tcgaaatcga
    10441 tccgaatacg caggcaatcg tcgataccgt cgaacggctg atggcgcaaa gatatgcaaa
    10501 gtgaggtaga gatggaattg aacgaatttc tcgataaagc ctatgccgtt ttgcggcgtt
    10561 tggatgccgt gcttccgccc gaacccgggc atacggattg gaacgcgctt gccttccgct
    10621 ggcagagtgc gggcaaaaaa ggttttttgg aacacttgcc cgatccgcac accttcccct
    10681 tggcaaggct ggcgggagtt ggcaggcaaa ccgaattgct ggtgcgtaat accgaacaat
    10741 tcattgccgg cagacccgcg aataacgtat tgatgagcgg cgcgcgcgga acaggcaaat
    10801 cctcgctggt caaagccctg ctgcacgaat atgcggataa gggattgcgc ctgatcgaag
    10861 tcgataaaag cgatttaatc ggcctgcctt acctgttgac gcttttgaaa gaacgtccgg
    10921 aaaaatttat cgtattttgc gacgatttgt cgtttgaaag cggcgatgaa acctataagg
    10981 cattgaaaac cgcgttagac ggcggtctgt ctcaacgttg cgccaacgtt ttggtttacg
    11041 cgacttccaa cagacggcac ctgatgcccg aatattttga cgagaatgcc ggtacgacgg
    11101 ggatgcgggg ggaaatccat cagaaagaag cggtggaaga aaaaatatcc ctgtccgacc
    11161 gattcggatt gtggctcagt ttttatccgt tcgatcaaaa cgattatctg gcggcggtgc
    11221 aaaattggct ggaagatttc ggcgtgccgt atgatgaaac cgcacagatg gcggcattgc
    11281 agtgggcgca gacgcggggc agccgttcgg gacgttccgc gtggcagttt gcctgcgact
    11341 gggcgggcag gctgccgaaa caacgggcgt tgtgagcgtt tcggggatta tttgaggcag
    11401 gaagaaagcc acatttgctg ctgttgcggt gtgaggatat ggaagaaccg gtgttggatt
    11461 tccaattcgt ccaccgcaaa atccataccg gacaaatagc ggctttcgac ataatcgcgc
    11521 gcctcgttcc gattaaaaac atccgaggaa atgatttcga cgacagaccg gcggcggctg
    11581 tgttcggaat gcataacctt caaacgcgcc ctgtcgcccg ccattttgaa ggcggcgcgg
    11641 attttacgca gttcattgtg ctgaccctgt gtcagtccga gccggcgtat gtcgcagttc
    11701 ggttgaaaat cgttcagggg gatataagcc gtggcggcgt gggaaagctg gcaggaaagc
    11761 aaagccatac ttaaaaaaga ggcggcaggt ttggcaaaac ggcagggagc aggcagtggc
    11821 acggcttttc ctcttgttgg aaattttagg aaatatacta gattgacgtg tgaaagatgg
    11881 tattatcgcg gttaactttt gttaataatg cgtacgtatg tccggcaggc agacataacg
    11941 gcggaatcgg aacgtatagt g
//
LOCUS       DAWFIT010000062        11039 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFIT010000062 DAWFIT010000000
VERSION     DAWFIT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.062"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WGQ85_001523"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WGQ85_001523"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0750917.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WGQ85_001524"
     CDS             complement(1612..2487)
                     /locus_tag="WGQ85_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0750918.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFLLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2489..3211)
                     /locus_tag="WGQ85_001525"
     CDS             complement(2489..3211)
                     /locus_tag="WGQ85_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0750919.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WGQ85_001526"
     CDS             complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WGQ85_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0750920.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPRDLNILNPKTIQAAV"
     gene            3788..4150
                     /locus_tag="WGQ85_001527"
     CDS             3788..4150
                     /locus_tag="WGQ85_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0750921.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKKDAATA"
     gene            4185..5048
                     /locus_tag="WGQ85_001528"
     CDS             4185..5048
                     /locus_tag="WGQ85_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0750922.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATIKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5146..6105
                     /gene="ttcA"
                     /locus_tag="WGQ85_001529"
     CDS             5146..6105
                     /gene="ttcA"
                     /locus_tag="WGQ85_001529"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0750923.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6199..9138)
                     /locus_tag="WGQ85_001530"
     CDS             complement(6199..9138)
                     /locus_tag="WGQ85_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0750924.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYSDNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9151..10842)
                     /locus_tag="WGQ85_001531"
     CDS             complement(9151..10842)
                     /locus_tag="WGQ85_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0750925.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2654 a         2955 c         2599 g         2831 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtgt attggggcat ccacactctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaag gcttgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccgtc
      421 cgatgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggatacga aaaaggccgc tacgacatcg tcaatccgat
      541 ggatcacgtc aacgccagcc aatccaccaa cgatgcctac cctaccggct tccgccttgc
      601 cgtgtattac agtatcggcg aattgctcga caaactgact gtcctgaaaa acgcctttgc
      661 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccgatg actgccggcc aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcaccc gccaactgct gttggaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tgacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtgcc ttgacctccg cccaataaaa caggcaggcg atggaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaaa aacagtgcgc ccgaaagcgt
     1741 ccccaaaaca atcacgccga tattcatacc ggcaaaaacc agcgtgggat tgtccttcat
     1801 catttggtgc gcggtgatgt aggttacgat gttcatgaag ttcaaaccgc ccaagagtat
     1861 gccgccgacc acactctcaa cgcgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcaaaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 taccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3241 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc tgggacggat gccgataatg
     3301 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3361 taaatatcca actttgtccg gatgccgaac ggcgttgtgc cgccgaccag atagcccgtc
     3421 cacttgtttg cctgtacggg cgtggcgggt tcaatgtgtt tcgcacccaa atgccgcgcc
     3481 agattgcggg ttgaaatctg cttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3541 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa tagtcgggca
     3601 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3661 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3721 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3781 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3841 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3901 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3961 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     4021 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagctcg
     4081 gctcgcccgt ccggagtggt gcgtcgaaat caaaataacc gccgtgaaga aagatgcggc
     4141 aaccgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4201 cctttttcgg cgtggtacgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4261 tttccaacga catcaataat cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4321 ccaaaggccg cgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4381 ttatggcgca agacttgctt gaagcaacga tcaaacgcct gcggatgttt gtcctgcgcg
     4441 ctaaagtcgt ttttgaaatc cttgaagatt acgccgtcga cgcggaattg gaagcatccg
     4501 ccgaaccgct tgccgcgcaa gaacccagtc tcgtctttac cgccgaatgc gtttcagacg
     4561 gcatctgcac cgtcgtcctg ccccatcggg gcattttgca catcgccccc aaaaacgcct
     4621 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgcagcggtt
     4681 acccgtggat atgcgcggct accaaagaaa ccgccgtcgc ccaaatgctc aaccagcaca
     4741 tcatcggcgg cgtacacttc aaaaaaggct gctatccggg acaggaaatc attgcccgtg
     4801 cccagtatcg cggtcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4861 aagcaggaac cctgctgacg gcagacggcg aagagaccgg catcgtactc gacagcgttc
     4921 aagattcgga aaactttacc gcccttgccg tgattaaatt ctccgccgcg caaaaagaac
     4981 tgaccgcccc aaacggcagt atcttcaaag cggttcatcc cttctttaaa accgaaaatg
     5041 cagaataatc cctctaatgc cgtctgaacc tttttcagac ggcattttaa aaccgcccct
     5101 gttttcggtt acaatgccgc atccccaatt tttcaaacca ccgccatgtc caaaaaaacc
     5161 aaacaagaac tggaaaacaa caaactcagc aaacgcctgc gccacgccgt cggcgacgcg
     5221 attaacgatt tcaacatgat cgagccgggc gacaaaatca tggtctgcct ctccggcgga
     5281 aaagacagct atgccctctt ggacatcctg cgccagctcc aagccagcgc gccgattgat
     5341 ttccaactgg ttgccgtcaa tctcgaccaa aagcagccgg gcttccccga agaagtatta
     5401 ccgacctatc tcgaaagcat cggcgttccc tacaaaattg ttgaagaaga cacctactcc
     5461 accgtcaaac gcgtattaga cgaaggcaaa acgacttgtt cgctgtgcag ccgcctgcgc
     5521 cgcggcatcc tctaccgcac tgcaaaagaa ttgggctgca ccaaaatcgc cttgggacac
     5581 caccgcgacg acatcctcgc caccttgttt ttaaatatgt tttacggcgg caaactcaaa
     5641 gccatgccgc ccaagctggt gagcgacaac ggcgaacaca tcgtcatccg tccgctggcg
     5701 tatgtaaaag aaaaagactt aatcaaatac gccgaattaa aacaattccc catcatcccg
     5761 tgcaacctct gcggctcgca acccaacctg caacgccaag tcatcggcga tatgctgcgc
     5821 gattgggaca aacgctttcc cggccgcatc gaatcgatgt tctccgccct gcaaaacgtc
     5881 gtcccttccc accttgccga taccgaactc ttcgactttg ccggattgga acgcggtcaa
     5941 accctgaaac acggcggcga tttggcgttt gacagcgaaa aaatgcccga acgtttttca
     6001 gacggcagcg aagaagacga aagcgaaatc aaaatcgcgc cgcaaaaagc cgaacgcaaa
     6061 gtcatcaaca ttctggcgaa caagccgaaa acctgcggcg cgtaaccccc cgccgtcaaa
     6121 aatgccgtct gaagcccgtt tggcgtttca gacggcattt taaaatcccg aagcagacgg
     6181 cttccaaaca tttgtttctc agtgtccgtt ttcagaatgc ccgcctgcgg gcatattttg
     6241 cccgatcagt tcggctatcc tctcgccgtc aaactgcgtt ttgaacacca ccttgatttc
     6301 tttggaaatc tcggcaaaca gcttttcggc atgtttgatt ttccgctctt cgctttttcg
     6361 caaatcttcc gccccgtcag tccctttggc ttcaatcaca aagttcagga tctcgccgct
     6421 tttggttttc acgatatagg caaaatcggg cgaatacgtg ccgccgccgg caacagggat
     6481 tttgatggag tttctcggta ttttggtaaa tacggttacg ccttcaattt ggttgttggc
     6541 gacattttca tgttctatat ccgaatcgta gaaaatctcg tcgaagagat agccggcggc
     6601 aggccggtgc tccgtatctt caaatctgcc caaatctgct tttttcaccg cgcgcggttt
     6661 gccgtcttta ttggtaaatt tggtcggatg gattttgctg ccgacaagcc ggtaatccag
     6721 ttcgaattta tggaaggaat gatgaagcaa aaaccggttg aagccgtttt tgatttgggc
     6781 gatggtttgc atattcaaaa aatcgccaat gttcagttcg ttgcggatgc ggtaaaacgc
     6841 ctgatgcaaa gtctgcatac ggatttttgc cgtttgtgcc agtttttcca gaaactctcg
     6901 gtaagtcatt gtgttgaaac ggataaaatc ttcatcttca atactgtcta tgcggcggga
     6961 aagcataagc ccgttgttga tataagcttc gtttaccgcc gtgcgtatgc ctgcctgcgg
     7021 gaatttggcg gcgttttcac gcaaataggc ggtaaataaa tcgacaaatt cggcttcatc
     7081 cttgattttg tactgcaaaa cggctttatg gtgaatcagc tcccacaagg ccttgagttc
     7141 ttcatatttg ccttcgcgca tgatgatggt gtctttgccc tcgtctttgg cgttgctgac
     7201 tttgcctttg tccaaacctt tggggaaggc ttcgggatag gcggctttta atttgtcata
     7261 gccgtctttg gtaaagtttt cattgtcgtc aatgatgccg tctgaaaaca gttggtttac
     7321 caataccagc ggtttgatat cggggtattt ttgcaatatt ttttgtttca gctcttcggt
     7381 aaactttttg gagatttctt cctgaaaaga attatcgttg atttcgccga caagctgctt
     7441 cacaaagtct ttttcgctgc tatcgacaaa ataattcagt ttgtacggca catcgcgcac
     7501 ccgcgccatc agctcgttta ccggcaggcg caggccgcgt ccgacttctt gcagcttgga
     7561 agtcgtgctg ccgctggaac gcagtttgca aatctggaaa acgttgggat tgtcccagcc
     7621 ttcgcgcagc gtccatttgg aaaaaataaa gcggcgcggg ttgtccaaag acaacagttt
     7681 ttccttatcg tgcaggattt cattgatttc ctgctcgatt ctatcgtcgc tgtctgtatt
     7741 gtctttggaa aaataaccgc cgtggcaggc ggatacatcg tccaacgtct tttgcaggta
     7801 atcgcggtaa aacgggtcgc tttccgtttt caggcggcgt gccgcttccg cgtgaatcca
     7861 gctttcaaat ttatctttca ggctgcctga aagctcgttg ccgctgcggt agcccgcaat
     7921 atcatcaata aaaaacagcg tcagcggctt gattttgggc tgtggcgcgc gttctgccaa
     7981 aagcgcgcgt tccagcttga aatgttcggc aaccgcccgc tgcatcatcg catcctgcac
     8041 cgtttgcgaa taggaataag ggttgatgac ggcacccgtt ttcaactcca agccgttgct
     8101 taacaccacc acggttttat tcattttgtc gattttcaaa tccgaaatag ccggatggat
     8161 ttgcgccaaa tcttcgcctt ttgccagttt gaacgtctgc tttttgtcct tttcgtttaa
     8221 ttcaaatttc gcttctttgc cgcccgacga caccagtttt accgccgcat ccatgccgcc
     8281 ctgcatttct tcctgaaaca cgcgcacgcc tttgaccagc ccgtcgttaa acgcgtctac
     8341 tgccgtcaaa cggtaaagca agttgtaata ttcatcgtta aatgtcgcac cgtagcgcaa
     8401 aatatattgc ggttttaagc gtttgatatt gccccacgtt ttcgcgctct cccgggtcgg
     8461 gaatttatgc ggttcgtcca caatcacaaa cgggcgcacg gcggccaatg catcaacggg
     8521 attgtcaaac aaatctttca atgccttgtc gcccgtatcg ttcatggacg acgaattaac
     8581 catgcccgcg ttaatcagca gcacatggat ttcctttttg ttttccgctt tgacaaattg
     8641 ctcaatcgtt atgggcgcat tggacttttt gcccttattc tttttcgcgc tttccaccac
     8701 ataggttttc aggcgcacgc cttcataatc gccgccgaaa tcctgttcaa aatgctctgc
     8761 caaagccttg ctttgcaaaa actgctgtgt tcccgcctta atggacaaag tcggcacgac
     8821 cacgataaat ttgaacacgc ccagccaacg gtgcagctcg aacatggttt gtgtgtaggt
     8881 ataggttttg cccgtgcccg tttccatgga aatatcaagg atattttggt cgtccgaacg
     8941 gtcggggaat cggccgtcta taccgttttg gctttgcact ttcaggatat tgtctgagta
     9001 ttgttttgca gcaaacaaaa gttcgggatt ttcgtctgcc gcccgatatt tgggcgttgc
     9061 cccgtcaaac acgcccaaaa ccgccgaaac cgcccgcatt tggtgcggct ggtttttctc
     9121 gtaattaaaa ccgctcatga attgcctccg tcaaaccctg ataaccacat tcaactcaat
     9181 ctctttttta ttggcataac cgcgaaccgc ctggtcaagt tcgtgctgca tggcgcttgc
     9241 catattgctg ccgaatacaa tcacgcggtt gggattgaaa tccgcatcgt cgtccagctt
     9301 gcggataaac gccaacaaat cggcggaagt aaaaccggca ttcatcagat acagccgttt
     9361 ttcgcacaga tacgccgtgt aagcccctaa ccgcacaggc tcaaccggcg tggtcagtgc
     9421 cgccccgtca tacagcgtcc aggtggtcag aagcgtttgc agctgttctt cgcttaattc
     9481 atcgttaagc ggcaaatccg gttgttcggg cgaaaaatcc ttgtccggat gctgcctgaa
     9541 attgtctgcc gtttgaaaga ttttgaagcc cgaatcgccc gtgtaatcgg gatgttcgac
     9601 gcggattttg gcggcggctt tttctatgcg ggctttggtg atgtcgaaga tggtcgggta
     9661 gcctgcttta cgggcttcgg atttttcagc ggttttttcg ggaagctgta cacagatata
     9721 gcggcggtta ccgttttgtc cttcggcgtt aagctgcatc acggcgtggg cggttgtgcc
     9781 gctgcctgcg aagaagtcta ggattaggtc agtttgattt cccttgaaag ttgcagaaag
     9841 aactaatctt ttcaataaat ctaatggttt tggtgtttca aaaggagaat aaccttttcc
     9901 agtttcaaac aactcttcta attcacgaat accatcttta gtaagatttt tacttatatt
     9961 tccaatattt aagatgctat gaaatttgga aaaatcatcg gatacttttc taagttttcc
    10021 aataattgtt ttcccataag acttcggaat taagatatat tcattatttt caatatcttt
    10081 atcaattttg tttttaaact caattccttt tatttcaata atatcttcgt aatgcaagtt
    10141 cccatatttg ccaaactgtt cgcgaacggc atcttcctgt atccaatata attcatcatt
    10201 gattacaatt tttttgccgc gtatatcttt tggtcttttt agattgtcaa aatgagtaat
    10261 atctttcgca taaactaaaa cagtttcatg cccatttaca acatattttg cattaccacc
    10321 accttctgat gtattaacaa ttaaatcttt aataaaattc ccttccccaa acacttcatc
    10381 acacaacaac ttcaactgcg ccgcttcgtt atcgtcaatc gagataaaaa tcacgccgtc
    10441 gtcctttaac agttcgcggg cgatatacag gcgcggatac ataaaggtca accatgcact
    10501 gtgcgagttt gagcctttgt cggtgaaatc taaaatccgc gcggcttcgt cttcgtcaat
    10561 attggctaag caggcaagtt cggctggtgt gaatttgcgg tcgtcctgat agacaaagcc
    10621 gtctgatccg gtgttgtagg gcgggtcgat gtaaatcatc ttcacgctgt ttgtgtaggc
    10681 gttttttaag tgtttcaaca cttctagatt gtcgccacga atcagtaggt tttggctgcc
    10741 tgcattttcg ggtttggcgt tgtgcgtctt gtcttcactt atcagggttt cgggcggcag
    10801 gttgcgaagc aggcgagcat atgatttgcc cagccagttc atttcgtaaa attcgcgtcc
    10861 gatgtcggtc tgtggcgcaa tttcggcttg taatctgtcg ataaggaaat ttccgtctgc
    10921 gtcaaaacag gcgggaaaca gttttttgag ctgttcgagt tgggtagagt tggcggtaat
    10981 gccgtctgaa gtgtagattg cctcggtgtt caccccggct gtgtcgatta gatcagggg
//
LOCUS       DAWFIT010000063        10949 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFIT010000063 DAWFIT010000000
VERSION     DAWFIT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.063"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..104)
                     /locus_tag="WGQ85_001532"
     CDS             complement(<1..104)
                     /locus_tag="WGQ85_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0750926.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPI"
     gene            291..473
                     /locus_tag="WGQ85_001533"
     CDS             291..473
                     /locus_tag="WGQ85_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750927.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(535..1536)
                     /locus_tag="WGQ85_001534"
     CDS             complement(535..1536)
                     /locus_tag="WGQ85_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0750928.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGGVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1613..5446)
                     /locus_tag="WGQ85_001535"
     CDS             complement(1613..5446)
                     /locus_tag="WGQ85_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0750929.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSNGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDETAVQTAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKERWDWLLANLNAPLADYKARYGAS
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLQIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5707..5803
                     /gene="ffs"
                     /locus_tag="WGQ85_001536"
     ncRNA           5707..5803
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGQ85_001536"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5970..7661
                     /gene="dld"
                     /locus_tag="WGQ85_001537"
     CDS             5970..7661
                     /gene="dld"
                     /locus_tag="WGQ85_001537"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0750930.1"
                     /translation="MSASQLLSRLTQTVGEKYIITAPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKIGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7793..7900
                     /locus_tag="WGQ85_001538"
                     /pseudo
     CDS             7793..7900
                     /locus_tag="WGQ85_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7956..8327
                     /locus_tag="WGQ85_001539"
     CDS             7956..8327
                     /locus_tag="WGQ85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0750931.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8482..9030
                     /locus_tag="WGQ85_001540"
     CDS             8482..9030
                     /locus_tag="WGQ85_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0750932.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9165..10256
                     /locus_tag="WGQ85_001541"
     CDS             9165..10256
                     /locus_tag="WGQ85_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0750933.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10714..10884
                     /locus_tag="WGQ85_001542"
     CDS             10714..10884
                     /locus_tag="WGQ85_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0750934.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2527 a         2986 c         2980 g         2456 t
ORIGIN      
        1 tcgatcggct gccaatcaat cacctggtcc aacttcaata gcgggaagcg gttgatgtgt
       61 ttggcaatca tggcttgggc ggtttgccgg aagaaggtgc tcatgagaaa tctcctaaat
      121 gtcttggtgg gaatttaggg gattttgggg gattttgcaa aggtctctat ttgtactgtc
      181 ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc gtgcgtttgt
      241 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      301 atgtaatcgg cttttgctcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgtcggcg
      361 cattcggtat gcaggaagac gataccccgc aatcggatta tgaaaaacgt ttgggcttgg
      421 gtgcgaagct gaaaaacaaa aatacgccgg atacggaaga aaagcagcaa taaagatatg
      481 aaatgccgtc tgaaagattt tcagacggca tttgctttga agcatgggag acagtcaaac
      541 atttatctca tgctcaaatg cgttcaggat tcggcggact tcatccgcat ccttaaccgt
      601 tcggacgcgg tcaaacagcg tttgcgccgg gtcgaacgtt tttttcatca tgcccaacca
      661 ttgcttaaga cgggcgatcg ggtatttatt gtttgcctct tttgtcaggc acagctcgaa
      721 aaactgccgt atccatttag aaacttcggc aaaatccgtg tctttgaccg gcccgccgtt
      781 ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accccgccgc gaccgagcat
      841 cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc tgaaaacgtc
      901 gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc attcccaatg
      961 cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc cgcatgcgcc
     1021 cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt aaccgagccg
     1081 catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt tgacgatgtg
     1141 gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt tgacggtggg
     1201 cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg cttccagcgc
     1261 attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg gcgtgccgga
     1321 aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg atcgggaatg
     1381 cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc caatacgcgt
     1441 cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga tgattcgggt
     1501 tttttgtttg ggttcggttg tttgtccgtc aatcatgatt cgggtaggta tttgattcaa
     1561 acaatgccgt ctgaaggctt tcggcgttca gacggcattt ccgtgtccgt tgttacagca
     1621 atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc cagttttcgc
     1681 cgaggatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg ttgtcgtcgg
     1741 tgtagcggct caagccttgc aggcaggctg ggcaggaagt aagcattttg acgggttcac
     1801 cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaat ttgacttggg
     1861 tggcgatatc gggtcgtttg acggcgaaca taccggactc gccgcagcag cggtcgctta
     1921 agacgacttt ctgccccatc aggctgctgg ccatttgggt ggcgttcatg gttttgatgg
     1981 gggtgtggca ggggtcgtgg tagaggtatt gctgaccttg cacgccgtcg agtttcacgc
     2041 ctttttcgag caggtattcg tggatgtcga tgatgcggca gccggggaag atttcttcaa
     2101 agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg gtcttaatgt
     2161 cgaggtagtt gagggtgttt gccatacggt ggaaagccac gcggttgttg gtgctcattt
     2221 cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac atatagccgg
     2281 gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca agtccgattt
     2341 ggctgaacag gcgttccgaa ccgcaacccg ggaagtagaa cacggcttcg gcatcttcgg
     2401 gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc aataaggagc
     2461 gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg ataacctgtt
     2521 ctttaattgg ggctttgccg acggttgcct tgggttcggc tttttgcttt ttcgtgccga
     2581 tctgaagaag cttgccgatt ttgtaggcaa agttttgcgc tgggaagccg gtctgtatca
     2641 tggcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac gccataccca
     2701 tagctgcggc aggcgcaaat cgtttgtggc cggaatcggc aaggtagttg cggacggcta
     2761 cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg tggcacacgg
     2821 tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg ccgcggcggg
     2881 tttgttcttc gtataagaag gcttcggtca gcaagcccac gccgaggatt ttgttgcgcg
     2941 gactgtacag caggttggca cgcggaacgt gagtagagca gacgggtttg catttgccgc
     3001 agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtttgatttt tccataatca
     3061 gcgattccgc gcccaacagc tcgaaggacg gcgtgtaggc gttgcgtaag tccgagcctt
     3121 tcatcagttt gtggcggttg aaggtgtgtt tggggtcgac ttggtttttg tagtcccaaa
     3181 acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt gatgccgatg ccgtgttcgc
     3241 ccgaaatcac gccgccgagc gaacgggcga ttttcataat gcgttcgact gagcggtatg
     3301 ccgtctgaag catttcggca tcgtctgagt taaccggaat attggtgtga acgttaccgt
     3361 cgccggcgtg catatgcagg gcgacaaaga cgcggctgcg tacggttttg gcgtggattt
     3421 ttcccaagcc ttggataatt ttggtgtcgg ttttgccgct gaagatttca gaaagcggct
     3481 tcattacgtc cgctttgaca gacacgcgca ggcggaaatc gcggaatgcg gtaaagcagc
     3541 tttcattgtc tttggcttcg ggcgcggcgt ggacggatgc gccatagcgg gctttgtagt
     3601 cggcaagcgg cgcgttcaaa ttggcgagca accagtccca acgttctttg acggcggaaa
     3661 cgtgggcaag ggcgtgtttg ccgcgttctc ccaaaagttc ggcggtcggc aggtccgtac
     3721 ccattttgtc gatggggagt ttgcccgaca gatattgctc caaggcggcg cagagtttga
     3781 gtttgttttg gatagaaagc tcgatgttga tgcgttcgat gccgtctgaa tactcgccca
     3841 gccgttccag cggaatcacc acgtcttcgt tgattttaaa ggcgttggtg tgtttggcga
     3901 tggcggcggt gcggctgcgg tcgagccaga aggttttacg cgcttcgggc gacacggcga
     3961 taaagccttc gccgtcgcgt gcgcgggcga gttcgcagat gtgttcggcg gctgtctgta
     4021 cggcggtttc gtcgtcggaa accacgtccg ccagcaagac cattttcggc cgccccttgc
     4081 ctgccgcttt ggtggcgtag ccgacagcgc ggacataacg ccagtccaaa tgctccaaac
     4141 cggccagtcg tacgctttca tgggcaagca aaaagtcgcg gatttcgaca atagatggcg
     4201 tggcggtggc aaccgtgccg aaaaactcca tacacacggt gcgcgtgtgt ttcggcattt
     4261 tgtgcaatac gaaggcaacg ctggtgatga tgccgtccgt gccttctttc tgcacgccgg
     4321 gcagtccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg cggaacttgt
     4381 ggccggggat ttccaagcgt tcggttttaa cgatattgag gccgtttgaa tccagcgtgt
     4441 gaacgtcgaa cacggcggtt tcttcatcgt ggattttgcc gaaattgtgg cgcacgcgct
     4501 cgatgcgcag ccattcgcct tgagggttga ccatctgcca gtaggcgagg ttgtccaacg
     4561 ccgtccccca cagcacggct tttttgccgc ccgcgttcat cgccacattg ccgcccacgc
     4621 aggacgcgtc ggcggaagtc ggatcgacgg cgaacaccaa acctgcctga tgcgcggttt
     4681 cttccactcg tcgggttact acgcccgctc cgcaccggat aatcggatgc ttgccgtcca
     4741 agcccgccag ctcaacgtat tcaacaccac gatgcttgtc gagtttttcg gtattgatga
     4801 ccgcgctgtt cgcgtccaaa ggtaccgcgc cgccggtata acccgtgccg ccgccgcgcg
     4861 ggataataac caagtccaat tcgattaagg cgcgcaccaa aggcgcgatt tccgcctccg
     4921 tgtcgggatt gacgacgaca aaaggatatt cgacgcgcca gtcggtcgca tcggtaacgt
     4981 gcgttacgcg cgccaatccg tcaaacatga tgttgtgcgg cttggtaatt ttgcttaagc
     5041 gttccaaaat ttgatggcgt ttttggatgg tttcatcaaa actgctatca aaacgctcca
     5101 ctgctttttc tgctgcggca accaacacat cgacttgccg attatcgtcg cggcgtttgc
     5161 ggatttcgtt caacctgtgg cgcatttcgc gtaccagcgc ggcgcggcgt ttcgggtgct
     5221 ccagcaaatc atcgaccaga tacggattgc gcacgaccac ccaaatatcg cccaatactt
     5281 caaacagcat tcgtgccgaa cgcccggttt tacgctgtcc gcgcaggtct tgcaggattt
     5341 gccacgcttc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag gatgtatagt
     5401 tgtaggggat ttcccgaata cgctgcgggg cggtagtcgt ggtcatggtg tgtccgtgtt
     5461 tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgggttt gaaaaggcga
     5521 taatgtagtt caatttttga tatttttcaa tgccgaaaca ggaaataccc ttatagaaac
     5581 aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta ggtggtgttt
     5641 ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat aatgccgttt
     5701 ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc ggaagccagc
     5761 agcccactcc ggtgcgccag tgccgggggt ttggcccgcc gccctatttg aaacgccgga
     5821 gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg ttcagacggc
     5881 attttcttta ctttgccata acgtataact acaatgtgaa aacaattcca atgtgcaatg
     5941 cacctgccga caattttaag gaaactgcca tgagcgcatc acaattactg tcaagactga
     6001 cccaaaccgt aggcgaaaaa tacatcatca ccgcccccgc gaagaccgaa caataccgcc
     6061 aaggctaccg cttcggcgag ggcagggcgt tggcggtggt gcgccccgga accattctgg
     6121 aaatgtggaa aattctgcag gcctgcgtcg aagcggacgt gattgtgatt acgcaggcgg
     6181 cgaataccgg tttgacgggc ggctcgaccc ccgacggcaa cgattacgac cgcgacatcg
     6241 tgattgtgaa caccatgcgg atgaacatca tccaaaccat caacaacaac gaacaagtcg
     6301 tctgcctgcc cggctcgacc ctgaaccagc ttgaattgct gctgaaacct ttggggcgcg
     6361 aaccgcattc ggttatcggc tcttcctgca tcggtgcgtc cgtcttgggc ggcgtgtgca
     6421 acaactccgg cggcgcattg gtgcagcgcg gccctgccta caccgaaatg gcactgttcg
     6481 cccaaatcaa cgaagaaggc aggttggaac tggtcaacca cttgggcata gacttgggcg
     6541 acacgcccga agaaatcctg accaacctgc aaggtcatca ttatcaaaac aaagacatca
     6601 aacaagacgc gggcaaaggg cacgaccacg cctattgcga acacgtccgc caagtggacg
     6661 aaccgaccgc cgcgcgtttc aatgccgacc ccgcccgcca ttacgaagcc tcgggctgcg
     6721 cgggcaaact gatggttttt gccgtccgtt tggacacctt cccgcaagaa aaacaaaccg
     6781 ccgtgttcta catcggcacg aacgacatca acgagctgac cgacatccgc cgcgccgcct
     6841 tgggcgaatt tgaaagcctg cctgtttccg gcgaatacat ccaccgccac gctttcgaca
     6901 ttgccgacgt gtacggcaaa gacacgtttt acgtcatcaa aaaattcggt acgcaccaac
     6961 tgccgaaatt atttgacttg aaggcgcgcg tggacaggtt cggcaaaaaa gccagcttcc
     7021 tgcccaaaca tttttccgac aaggccatgc agttcgtcag caaatacctg cccgaccacc
     7081 tgcccaaatc catgcgcgat taccgcgaca aatacgaaca ccacctgatt ctgaaaatag
     7141 gcgggaaagg cgtggatgag gcgcgcgcgt tcttaaaaga atattttgca caccacagcg
     7201 gcgcgttttt cgagtgcaac gccgaagaaa cccaagccgc gatgctgcac cgtttcgccg
     7261 tcgcctccgc cgccatccgc taccgcgccg tgcatgatga cgaagtggaa gacttggtcg
     7321 cgctcgacat cgccctgcgc cgcgacgatc gcgactggtt tgaaaaactg ccgtcggaaa
     7381 tcgacaataa aatcatccat aaactctact atggacattt catgtgccac gttttccatc
     7441 aggattacat catcaaaaaa ggcaacgact gcatggcgtt ggaacacgaa atgctgcatc
     7501 ttttagacca acgcggcgcg caatatcccg ccgaacacaa cgtcggtcat ttgtatgaag
     7561 ccaaacccgc gctcaaacag ttttaccgca aacttgaccc gaccaacagc ttcaacccgg
     7621 gcgtaggcaa aaccagcaaa aagaaaaact gggcggaata agcgcgtccg ctttgaaggc
     7681 aggcaatgcc gtctgaaggc aaaacgccgt tcagacggca ttgtcgggtt tgaagttccc
     7741 cgtcgggtaa tcgctttcaa accgtatgcc gacacacggg cgcgcccctc ccaaatatag
     7801 tggattaaat ttaaatcagg acaaggcaac gaagccgcag acagtacaga tagtacggca
     7861 aggcgaggta acgccgtact ggtttaaatt taatccacta taaagtctga tcctaccttc
     7921 ccaaagggca gggtttcaac cgaaaaggaa acacgatgaa accatacaaa atctacaccc
     7981 atcccgccct gccgccccaa gccgtcaaac aaggctggtc atggccgggg ctcttgttcg
     8041 gcacgctgtg ggcgtgtttc aaaagaatgt ggggtttggg gctggggctg acgggcgcga
     8101 tattcgtgct ggctgtgttt gcccaactgg tttacggcga cacgcccgcc acagactcgg
     8161 cgtttaacgt attgggcttg gcggtttccg tctggttcgg cgcaaagggc aacagccttt
     8221 atgcacgcca ccttttatcg cgcggctata ccgaattgcc cgaaacggtt gaggcagcca
     8281 atccccaagc cgcattggcg caatatttcg ggcgcggggg ccgataagcg cgttttcttt
     8341 tccatcgtgc cgtctgaaaa atgttcagac ggcatttctt ataacctttc gtttgcagca
     8401 cataaccaat cagtctttcc tattccgttc cgaaatgcat accatgcgcc cgtacccaat
     8461 atttgcgaac aaggaaagaa aatggcacgt ttaaccgtac acaccctcga aaccgccccc
     8521 gaagccgcca aagcgcgcgt cgaggcggta cttcaaaaca acggctttat ccccaacctt
     8581 atcggcgtat tatcaaacgc ccccgaagcc ttggcgtttt accaagaagt cggcaagctc
     8641 aacgccgcca acagcctgac cgccggcgaa gtcgaagtaa tccagattat tgccgcccgc
     8701 accaaccaat gcggcttctg cgtggcaggg cacaccaaac tcgcaaccct gaaaaaactc
     8761 ctttccgaac aatccgtcaa agccgcgcgc gctttggcgg caggcgaatt tgacgatgct
     8821 aaactcggcg cgctcgccgc ctttacccaa gccgtaatgg caaaaaaagg cgcggtatcc
     8881 gacgaggaac tcaaagcatt ttttgatgcg ggctacaacc agcagcaggc agtcgaagtc
     8941 gtgatgggcg tagccttggc aactttgtgc aactacgtca acaacctcgg acaaaccgaa
     9001 atcaaccccg aattgcaggc ttacgcctga tacggcaaac cgcccgaata tcgggcggtt
     9061 tttcaaaaac accccctcaa acaaaaacaa gccgcctaag cggcagggaa gcagccgcgc
     9121 atcaggcgcg cccccgattt ccccgcaaac cttaaggaac aaagatgaac gcccaaaccc
     9181 tgattgccaa cgttgccgaa ttcgtcaaaa ccaagctcaa acccatagtg gacgacatcg
     9241 accgcaaagg atactacccc gaagcattta tgcgcgaact cggcgcaatc ggtggattcg
     9301 gcgcagtcgg tacagaagcc gaaggcggca acggcttggg tttggcaacg caaatcgccg
     9361 tattgcgcga aatcggcaaa gaatgcggcg caacctcttt cagtgcgtgg tgtcaggcgg
     9421 cttgcgcgtg gtatctgcac caaacgccca accgggccgt caaagacaaa taccttgccg
     9481 acatcctgca aggcaaagta ttggcgggta cgggcatgtc caataccgtc aaacaccttg
     9541 ccgacatcga aaaacacaac ctccaagccg agcgcgtgga gggcggctac acagtcaacg
     9601 gcgcgctgcc gtgggtgtcc aacatcggcg aagaccacat ttgggcgaat accgcccaaa
     9661 tcggcggcgg ctacgtcatg ttcatcacag gcggacaatg ggaaggcgta agcctgcaaa
     9721 actgccccga attttgcgcc ctcgaaggca cacgcacctt cagcctgaac ttcaaagacg
     9781 tatttatccc cgacgaagac atcatcgccg cgcccgaaca gtttgccgac tacatccaaa
     9841 gcatcaaagc aggctttatc ctcctgcaaa tcggcatcgg cgcgggcgtg atagacggca
     9901 gcctcggcat catccgcctc gccaacgtcg tcaacgccga agtcaaccgc tatctcgacg
     9961 acggctacga cagcctcaaa acaaggctgg acggcgcgtg ggcagaaacc gaacggctcg
    10021 ccggattggc ttggagcggc acgcccgaca accttaccac cctcaagctg cgcgaagccg
    10081 ccgccgtact gacacttgcc gccgcccaat ccgccgccct gcattccggc gcaaaaggct
    10141 acctgatgcg gagtcccgcc caaaggcgcg tccgcgaagc aatgtttgtc gccatcgtaa
    10201 ctcccgcgat caaacacctc cgcaaagaaa tagcggcaat cgaagccgca aaataaacag
    10261 gcggaaaaaa ccgaaacccc catcgcgtca ttcccgcgaa agcgggaatc cagcccccaa
    10321 cgtaacagga atccatcgga aaaaacagaa accctcaaac cgtcattccc gcaaaagcgg
    10381 gaatccagcc ccccctaacg caacaggaat tcatcgaaaa aaccgaaacc tccaaaccgt
    10441 cattcccacg gaagtgggaa tccagtaatc gaaaaaccac aggaatctat cgaaaaaaaa
    10501 ccaaccccaa aaaaaccggg cggataccgc accattctcc caaaccctga tttatagcgg
    10561 atgaacaaaa atcagggcaa ggcggcgaag ccgcagacag tacaaatagt acgggtgctt
    10621 cagtacttta gagaatcgtt ctcttcgagc taaggcgagg caacgccgta ccggtttttg
    10681 ttaacccact atacaaaaaa caaggaaaca caaatggcgc aatatatgtg cggcccctgc
    10741 ggctggattt acgatgaaga acacggcgac cccgaacacg gcatcgcccc cgggacaaag
    10801 tttgaagaca tccccgacga ctggaaatgc cccgaatgcg gcgtgggcaa agaagatttc
    10861 tacctgttgg atttcgtgat atagcggcag ccgcaccctt agggaaaacc ccgtcatccg
    10921 gcgttccgcc tgcggcggca gggaacacc
//
LOCUS       DAWFIT010000064        10727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFIT010000064 DAWFIT010000000
VERSION     DAWFIT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.064"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(293..505)
                     /locus_tag="WGQ85_001543"
     CDS             complement(293..505)
                     /locus_tag="WGQ85_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750935.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(517..798)
                     /locus_tag="WGQ85_001544"
     CDS             complement(517..798)
                     /locus_tag="WGQ85_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750936.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(836..1099)
                     /locus_tag="WGQ85_001545"
     CDS             complement(836..1099)
                     /locus_tag="WGQ85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750937.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1127..1700)
                     /locus_tag="WGQ85_001546"
                     /pseudo
     CDS             complement(1127..1700)
                     /locus_tag="WGQ85_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1675..2019)
                     /locus_tag="WGQ85_001547"
                     /pseudo
     CDS             complement(1675..2019)
                     /locus_tag="WGQ85_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2012..2887)
                     /locus_tag="WGQ85_001548"
     CDS             complement(2012..2887)
                     /locus_tag="WGQ85_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0750938.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2932..3222)
                     /locus_tag="WGQ85_001549"
     CDS             complement(2932..3222)
                     /locus_tag="WGQ85_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0750939.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3234..3413)
                     /locus_tag="WGQ85_001550"
     CDS             complement(3234..3413)
                     /locus_tag="WGQ85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0750940.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3527..3904
                     /locus_tag="WGQ85_001551"
     CDS             3527..3904
                     /locus_tag="WGQ85_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0750941.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVSKGAEKKAAENTQVSATHKTA"
     gene            3933..4178
                     /locus_tag="WGQ85_001552"
     CDS             3933..4178
                     /locus_tag="WGQ85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750942.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4412..5380
                     /locus_tag="WGQ85_001553"
     CDS             4412..5380
                     /locus_tag="WGQ85_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750943.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5377..5859
                     /locus_tag="WGQ85_001554"
     CDS             5377..5859
                     /locus_tag="WGQ85_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750944.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5949..6452
                     /locus_tag="WGQ85_001555"
     CDS             5949..6452
                     /locus_tag="WGQ85_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0750945.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6561..6893
                     /locus_tag="WGQ85_001556"
     CDS             6561..6893
                     /locus_tag="WGQ85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750946.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            6890..7300
                     /locus_tag="WGQ85_001557"
     CDS             6890..7300
                     /locus_tag="WGQ85_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0750947.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            7315..8037
                     /locus_tag="WGQ85_001558"
     CDS             7315..8037
                     /locus_tag="WGQ85_001558"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0750948.1"
                     /translation="MNCCWGLSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPR
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGQ85_001559"
     tmRNA           complement(8358..8720)
                     /gene="ssrA"
                     /locus_tag="WGQ85_001559"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8783..9388)
                     /locus_tag="WGQ85_001560"
     CDS             complement(8783..9388)
                     /locus_tag="WGQ85_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0750949.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(9866..10051)
                     /locus_tag="WGQ85_001561"
                     /pseudo
     CDS             complement(9866..10051)
                     /locus_tag="WGQ85_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10139..10727)
                     /locus_tag="WGQ85_001562"
                     /pseudo
     CDS             complement(10139..10727)
                     /locus_tag="WGQ85_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216327.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2558 a         2494 c         2728 g         2947 t
ORIGIN      
        1 gcgcccgctg ccgcctaaaa ggcgaccgga tgcctgatta tcgggtatcc ggggaggatt
       61 aagggggtat ttgggtaaaa ttaggcggta tttggggcga aaatagacga aaacctgtgt
      121 tggggtttcg gctgtcggga gggaaaggaa ttttgcaaag gtctcagagt gagtttattt
      181 tggggcggcg gcgggtcggg ggcaagcggc gtggggcttg gttgtggttt ttaggtttgg
      241 gggtgggtaa aaaatgccgt acccggcggg caggcttcgc actctgaagg tttcagacgg
      301 cgtttgtttt ttctgtccaa tcgaggaact gccgccattt ttccagcgac atatcggtcc
      361 gccgcgtttc ggtatcgggt ttggcttccc agcggcctac ctgtatgtag tgcttcaccc
      421 cgacgatttg cgccaactcg gcctgtgtca gtttgcagcg gttgcgcagg gtgcgaaggt
      481 tgtaaggcgt gtagccgagt tccatgtcgt tgcggtttat tttgtttcgc atattttttt
      541 gactgcccgg cggcaggttt cggtaaggac ggcggcaaat cgggcttcgt ctgccggttt
      601 tccgtcgaaa aaaataaaat cgtaaagggt gatgccgttt tcggaatggc ggtaatgccc
      661 gcccgggcat tcgctgtctg tctgcctgtg gattttggca atcaggcggg gatagcggca
      721 atgggtaatg aaacggcttg cgccgtcttg cccgataatc cattcggggt agcggttgaa
      781 tagtgcggct ctgttcattt tgttcggggg ataaagcccc tcgcggggct tgtggtcagg
      841 caaatttgaa tatcagtgcc aacacggcgg cgatggcggt aaccagcccg gtggccgcca
      901 tcatgggata ccatcgggat tcttgggcta tttttacgga ttcggcgttg attttgtgcg
      961 cgtctgcaat gattttggcg atttcggcat ctacttttct cagactggag tgtttcattt
     1021 cttgttcgat tatgttcatc gtacttcctt tcgtttttgg cggttgccgc cgcttgtcgg
     1081 atggtaggat gtctgccatg tgtatatatt tatatattga taccttttag gttttattgc
     1141 aagtgttttg ggcggcggct tcgtatgctt ggcggtggcg gcggctgtac cattcggcga
     1201 gttcgcgccg ctcttggagg cggtagctgt cggcggtcag gtaatggtgc aggcttgaga
     1261 agccggcgcg ttggagggcg gcgcggctga tgtggccgag ggcgaggtct gttgtgaggg
     1321 tgtcttttcc ggcggtcagg ctgatgttgg tgaagaggtg gcggaatccg tgcattgtgt
     1381 gtttggattt gccgggggtg ctgccgtcat agcccagtcg tcggatggcg ttgtgggcga
     1441 atttgatgct gatgtggtcg ggatgggggg gcgggtttgc gccgtgggcg gatgcagggg
     1501 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1561 tccgacagtg ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1621 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1681 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1741 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1801 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1861 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1921 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1981 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     2041 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     2101 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2161 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2221 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2281 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2341 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2401 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2461 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2521 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2581 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2641 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2701 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2761 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2821 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2881 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2941 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     3001 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     3061 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3121 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3181 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3241 agtcccagct tcttggcaat ttcatgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3301 accaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtt
     3361 tggccgtttt gggcaaaatt ttcttttagt ttttcaacag tatagccata gatagccctt
     3421 tcccattgtg ataaaatagt gaactattta tgcactagaa attaattagt gcattaccaa
     3481 tgcgtgaatt atgagatttt aaaatctctt ttgcaaggat tattttgtga tttctaaatc
     3541 tctttttggt aatcgtttga aagaaaaaag aaagttttta gggcttaccc aagcacaagc
     3601 tgctgaaaaa gcaggtattg agcgcgaaac atggggcaaa tacgaacgtg gcgtatttat
     3661 gcctagtgga gatgtattgc tctctttttt gaatatggga atagatgtga gcagcttatt
     3721 tgctgctgaa cagggaatta ggccgtctga aaattctgaa attagtaagg aagagtcaga
     3781 gttattaggg tattaccgcc aagccagcga taacggcaaa tttgtcattc tcagtgtgtc
     3841 aaaaggcgca gagaaaaaag cagccgaaaa tactcaagtt tcagccacgc ataaaacagc
     3901 gtgattttta aatccaaaat ggagacagtc ttatgtttga ctttgaacaa caaatcaaat
     3961 ggggtgagcg agcggaagaa atcgttaaag aggcagccac ccagaacaat atcgaaattc
     4021 ccgaaccttt ggcttcggca ttagctaagg cggtaaaagt gcattatcta agccaagctg
     4081 gtgtgttttc tttggtagaa gcctatgcag acacggtaaa tccaactgaa aaagaagtag
     4141 attaccaagc aatcggcaaa gagctatttg aaaaataaaa aatgccgtct gaaattttca
     4201 gacggcattt aggacactcc gacagtcggg ctgctccggt gttctgaaac ggatattaag
     4261 caattgaagc ggccgtgcat tttaaaacgc attaaaaact accccgacat acaggtcatc
     4321 gggcaggtgg ttcaggtgtt gaaagatttg aattgatgcc gtctgaattt attttttcga
     4381 cacagatatt aaaagagaaa ggactgtaca aatggcaaaa aaaactacta aaactattca
     4441 ttatttacgt gcgcagcaaa accatagctt gttcgattta gaggtagcat taagaagggt
     4501 tttgtcagct gctcctactg ttcaagatac acaaattgaa caatataatc agcttaccca
     4561 aatcatgcat agaactttgg atcctaaaga tgtaaaaggc aattcaattg gtggattact
     4621 gatacacatt ggcagcggta caaaagatga acatatccgc actatgagca atagacctgt
     4681 acagcaagat gatcatggtg gaacacaagc tcctccaagt ggatattcct ttttgcgtaa
     4741 agaggctttt ttgtatattg ttggacacca tgtaattttt tgcggacatg gttttttgtc
     4801 agcatcaacg gttgcttctt atttaagttt acttagcaat aaattgagag agaacaatcc
     4861 ggatattgtt caatttaata ttgaattcaa agcagtaggc aattgcgaca agttgtcatt
     4921 gatacaacaa catggggtaa aaagtattct tttagatgct tcggcatatc aattatccag
     4981 aaacagactg tatcaagata gccgttcaac cattgctaaa gcattaggga aagttggtag
     5041 tgtatttaca agcgaattga gtgatgagga attggaagca caatccgaaa ttcatatcaa
     5101 tttagaagtc ttgctaaacg gtaattcgag agccagtatt gaagcgcaat cgctgatgca
     5161 ggagcaggca gaggaaatta ttgatgatga aacggttaat caaggatttt ccattaccac
     5221 gcaacaaggt gaagtgattc agccctctga tgtaaaactg tctaaatctg ttagaatagg
     5281 acgatacgat gaagccaact cgttactccc tgactcggca tttacagcaa tcagtgaata
     5341 tttcctagaa ctccaaagca gaaacttaac tgaacaatga aaaaatatct gcccttacta
     5401 cgttttttgt tgattgcttg tatatcagca tttttagcat ggaaagggca accttttgtt
     5461 catgggaacg agaaggccgt tgatttaatt attaacgtat ttgcaatctt agccggtttt
     5521 ttaattgcca ttatgacatt gtttagtgat atgaggtttg atgaagatgc aaattggcga
     5581 caaattcaaa ttcgtgaagg cgtacaagaa caacggtata taaaacattc cttacttttt
     5641 tacacatatc ttgccgtatt agtttgtgtt tttattgtca tcttgttggc tcataaagaa
     5701 gaatacaaaa atggaccagc cattttttgg cttgaacgga gctatttatt tttagcctgt
     5761 atttccattt tttattctgt attcttacct gggaatttaa ttaaaagccg aaaagaagag
     5821 tttaaaaaat taatggaaaa gaagaaaccc aaaatctaga acgagtttta acccccttta
     5881 aaatcccatt cagacgacct tttctaaaat ccctgtattg atttttaatt caatacaggg
     5941 atttttccat gtcagacgaa ttcaaccgat ttatcgaacg cgtcctctcc cacgagggcg
     6001 gttacgtcaa tcatcccaaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgca
     6061 ccgcacaggc aaacggctac aacggctcca tgcgcgccat gacgcgtgaa caggcaatcg
     6121 gcatttaccg taaagcgttt tgggagcgtt accgcgccga ccaaatgccc gaagcggtcg
     6181 cttttcaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctcc
     6241 aacgcgccgc aggcgtgccg gacgatggaa tcatcggcga aatcagcctc aaagccatca
     6301 attcccttcc cgaaaacgac cttttattgc ggttcaacgc cgagcgtctg gtcttttata
     6361 ccaagctggg tacgttcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
     6421 tgattcacgc cgccgcagac aatgccgatt gaggagcaaa ccatgtcaaa aaagtccatt
     6481 ctcgccctga tggccgcagc catgcagccc gatttcagcc acagcgacct aggcattcgc
     6541 tacgccatgc cgactcaggg atgttggacg caagcccacc gcaagagcgg ggtagccgcc
     6601 gcgaaacgcg cagccaaaaa aaagcgtcac aaataaccgc ctttttccga tggctgggcg
     6661 gcttggtctc taatccggcc acagggaaaa tcagccatac caaactttgg gcaaacgtcg
     6721 ccgcagccgc catgacttgg aagttcgtgc aggcggcgga cgcgcccgaa tggctttggt
     6781 gggcatacgg cgcgatggtc ggcgggtatg cattaatcaa acgcggcatc gccgtcgtac
     6841 cgcagttggc ggaagtcaaa aaatccgcaa atcaggaagg ggggcggcaa tgattgaatt
     6901 tgtccgagcc aaaaaacggc tgctttgggc atttgtgctt ttgcttgtgt ggacgtgcgg
     6961 ttaccgatac gccgccgaca aggccgaagc ggaacaaacc gccctgattg ccacctatcg
     7021 gcattcttct atggttgcgg cggaacaata tgccttgcag cttaaaaaag cgcaggacga
     7081 aaggcagcgg tggtacgact tttcccaaaa acaaggaaga aagcccgtga aaaaacagta
     7141 tccgccgcaa acgaaaaaag ccggctatct gaaaaccaag gaagaactgc ttgcggaatt
     7201 ggcttgcctt aaagcggaaa tggttgccct aaaaaagccc gatgccttaa tccatgggaa
     7261 agaagtgcgg cagaaagaac gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac
     7321 tgctgttggg gattgtcctt ctattaccaa ttggccgtcc aatcggcaga agacaaatat
     7381 gccgatttga aacggcatat ccatgatatt tatcgacgac ataagggaag atacggctac
     7441 cggaggattg cggcagccat ccgtcacgca ggaacaccgg tcaatcacaa gaaagtcagc
     7501 cgtctgatgg cgaagacggg gctgaaggca gtgatacggc ggcgcaaata ccgctcgttc
     7561 aaaggagaag tcggcaaaat tgcgccgaat atcctgcgac gctgtttcca tgcagaaaag
     7621 ccgaatgaga aatgggtaac ggacgttacc gagttcaatg taggcggaga aaagatatac
     7681 ctttctccga ttatggattt gtttaacggg gaaatcgtca gttaccgtat tcaaatccgc
     7741 ccgactttcg atttggccgg cgagatactg aaaggtgcgc cggagaaacc gagatcgtct
     7801 gaaaagccga tactgcattc ggatcaaggt tggcaatatc agatgttttt ttatagtgga
     7861 ttaaatttaa atcaggacaa ggcgatgaag ccgcagacag tacaaatagt acggcaaggc
     7921 gaggcaacgc cgtactggtt taaatttaat ccactataca acaacgatag aatcaagttg
     7981 aaattaaaag ggctgagcct tgttcagtac agaattcagt ccctgaaagc cgcttgatta
     8041 aactgtccga ctttttgggg tcagttcggc ttcggcattt ttttatccgt ttgggggtaa
     8101 cttgtttgga aagctgcaag cttataaata aaggattaca tttaagtttt gggtaacctt
     8161 tttaaaaaaa tgcgtgatga cttttgcatt cttaaggcgt tttttggggt aattcgtgaa
     8221 aagagttacc ccaaaagtta ccccataaat ggcgaaaact caagcatacg ccagcatatt
     8281 caaggcaaca aaaaagcctt gaaactgttg aagttcaagg cttttttgtg tttcaggata
     8341 ctgctgaaaa tagggtatgg tggaggcggg gggaatcgaa cccccgtccg aaagtcctct
     8401 acaaagcgtt ctacatactt agttgtgtct atttgaaaat cttatttcca tcatgccgac
     8461 caacaggcct tttggaaacc agttacctta aatctcattt cctgccaagt aacccggcag
     8521 gaaaccagtc aatgtaagat gacgttgcgg tggctttcgc cacacagccc attgaccgac
     8581 tgctgcaacg gctagcctta agcggctaaa gcgtaagttt cgtcgtttgc gactatttga
     8641 attcagtgtt ttacgggaat ctgagacccc ggtatgcccg catctgcttc gcaacccccg
     8701 tcgaaaccaa ggtcgccccc aaaaatggtt tgcaaattat acggatattg tgcggtgctg
     8761 ccaagtctgt cggagaaatt tgtcagtctt gctgccttaa tttgcgtttg agcaggatgc
     8821 ggacgcagcc gtcgttgcct tcccggggtt cggcgtaggc gagtacatcg gggtgttgca
     8881 tcagccagtt tcgggtcata tttttcagaa cgggtttgta gcctttggaa cctaatccgc
     8941 tgccgtggat gatttcgccg catacgccgc gtttttgggt gaatgcgatg aattcgttga
     9001 ggactttttg ggcttcttcc tgtgtgtagc cgtgcaggtc gacatcggta acgacgggat
     9061 agtatccgtt tttcaggcgt tggatgtcgt tttttccctg tccgtttttg ctgaagctgg
     9121 cgggcgggtc gttgtatgtg ctgccgatgt agaagtagtt ttcttcgtcg gcgcggttgt
     9181 ctttgggacg gactttgatg ggggttttgt cgggcggcgc ataatattgc tgccggtttt
     9241 ttaatgggga gacttgtccg actgcttgtg aaaaatcgaa atcctgttct tgtttttgct
     9301 tgtttttttc ggctgcctgt ttttctgccg cttctttttg ggcttgtttg cccagttgtt
     9361 tgaggatgtt ttggaagtcg gtattcatat tttttcctgt tatttgtccg atggctgttt
     9421 cgggcggggt tttaatttgc cggaatgttt gccaatcggg ggaggatgat tttgttgccg
     9481 tcgtatgttt tttgaaagtg tgattgtata tcaaaaagaa atgcggcaac cgtcggcagt
     9541 gttgattgcc ggaaatgcgg gccggtcgaa ccgatatgcc cgaacgcctg ataaagtttt
     9601 aaaaacctgc cttgcgaagc aggttaatgg ttttgccaat tttgaattgc cggaaacgcg
     9661 aaacacggaa atctgatgtt ttataaatgt tccgatacga actgcaaaat attggttgag
     9721 acctttgcaa aattcctttc cctcacgaca accgaaaccc caacacaggt tttcggctgt
     9781 tttcgcccca aatatctcct aattttaccc aaataccccc ttaatcctcc ccggataccc
     9841 tataatcagg catccgggct gccttttagg cggcagcggg cgcacttaac ctgttggcgg
     9901 ctttcaacag gttcaaacac atcgccttca gatggctttg tgcactcact ttaatcagtc
     9961 cgaaataggc tgcccgcgca tagcggaatt tacggtgcag cgtaccgaag ctctgttcga
    10021 ccacataacg ggtcttcgat aaataccggt tgagaccttt gcaaaattcc tttccctccc
    10081 gacagccgaa acccaaacac aggttttcgg ctgttttcgc ctcaaatacc tcctgatttt
    10141 acccaaatac ccccttaatc ctccctggat acccgataat caggcatccg gtcgcctttt
    10201 gggcggcagc gggcgcactt agcctgttgg cggctttcaa caggttcaaa cacatcgcca
    10261 cgggtcttcg acaaataccg gtcgcgtttg gtttgcgttt ccgtcagcgg acggttgcgg
    10321 caggctttgc gcataatgcc gtcctgcaac tgatgttctt ccagatgttg ccggttttcc
    10381 gcactgtcat agcctttgtc ggcatagacg gtcgtacctt tgggcagtcc ttccaacaaa
    10441 ggcggcaggt gtttgcactc atgggcattg gcgggggtga tgtgcagttt ctcgatatag
    10501 ccttccacat cggtacgggt atgttgtttg taaccgagtt tgtagaggcc gtttttcttg
    10561 atccaacggg catcgctgtc cttactcggt gtggtttggc cgctgatttg tccttcctca
    10621 tcgacttcta tagcctgacg ctgtttgctg ccggcggtct gaataatggt ggcgtcaacg
    10681 acggcggcgg atgctttctc tacttttagg cctttttcgg tcagttg
//
LOCUS       DAWFIT010000065        10689 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFIT010000065 DAWFIT010000000
VERSION     DAWFIT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.065"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..384)
                     /locus_tag="WGQ85_001563"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="WGQ85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(494..781)
                     /locus_tag="WGQ85_001564"
                     /pseudo
     CDS             complement(494..781)
                     /locus_tag="WGQ85_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(889..1098)
                     /locus_tag="WGQ85_001565"
                     /pseudo
     CDS             complement(889..1098)
                     /locus_tag="WGQ85_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1095..2150)
                     /locus_tag="WGQ85_001566"
     CDS             complement(1095..2150)
                     /locus_tag="WGQ85_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0750950.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(2256..2738)
                     /locus_tag="WGQ85_001567"
     CDS             complement(2256..2738)
                     /locus_tag="WGQ85_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0750951.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(2785..3921)
                     /locus_tag="WGQ85_001568"
     CDS             complement(2785..3921)
                     /locus_tag="WGQ85_001568"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0750952.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            complement(3918..4463)
                     /locus_tag="WGQ85_001569"
     CDS             complement(3918..4463)
                     /locus_tag="WGQ85_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0750953.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(4460..5935)
                     /locus_tag="WGQ85_001570"
     CDS             complement(4460..5935)
                     /locus_tag="WGQ85_001570"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0750954.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(6046..6411)
                     /locus_tag="WGQ85_001571"
     CDS             complement(6046..6411)
                     /locus_tag="WGQ85_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750955.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(6432..8342)
                     /locus_tag="WGQ85_001572"
     CDS             complement(6432..8342)
                     /locus_tag="WGQ85_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0750956.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(8361..9005)
                     /locus_tag="WGQ85_001573"
     CDS             complement(8361..9005)
                     /locus_tag="WGQ85_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0750957.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(9169..9987)
                     /gene="opc"
                     /locus_tag="WGQ85_001574"
                     /gene_synonym="opcA"
     CDS             complement(9169..9987)
                     /gene="opc"
                     /locus_tag="WGQ85_001574"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0750958.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            complement(10576..>10689)
                     /locus_tag="WGQ85_001575"
     CDS             complement(10576..>10689)
                     /locus_tag="WGQ85_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0750959.1"
                     /translation="ARAAYFGLIKVSAQSHLKAMCLNLLKAANKLSAPAAA"
BASE COUNT         2312 a         2352 c         3031 g         2994 t
ORIGIN      
        1 catagacggt cgtacctttg ggcagtcctt ccagcaaagg cgacaggtgt ttgcactcat
       61 gggcattggc gggggtgatg tgcagtttct cgatatagcc ttctgcatcg gtacgtgtat
      121 gttgtttgta accgagtttg tagaggccgt ttttctttgt ccaacgggca tctttatcct
      181 tactcggtgt ggtttggccg ttgatttgtc cttcttcatc gacttctata gcctgacgct
      241 gtttgctgcc ggcggtctga ataatggtgg cgtcaatgct ttctctattt ttaaaccttt
      301 ttcggtcagt tggcggttaa tcagttccag caattcggac agggtgtcgt cttgcgccag
      361 ccagttgcgg tagcggcata aagtatagtg gattaacaaa aatcaggaca aggcgacgaa
      421 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt ttttgttaat
      481 ccactatact gctcagttcg tcgaaacggc aaaacaggtt gaaatcgatg cgggtaatga
      541 ggctgtgttc gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga
      601 acatggacaa cagcgggtag gcgggacggc cgcggtggtc tcgaaggtaa cgggtttttt
      661 gacggttcag gtactgctcg atcggctgcc aatcaatcac ctggtccaac ttcaatagcg
      721 ggaaacggtc gatgtgtttg gcaatcatgg cttgggcggt ttgttgaaag aaggtgctca
      781 tgagaaatcc cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg
      841 tctctggttt tgtttctgac aggcaaaagc actgtttatt tggctgtcaa aaggatggtt
      901 aaggaaagtt atgcgcccct gaagcgggcc ccagataagg atggttgcgc cgacggcttc
      961 agacggcatt ttggcggcgg tgttgggttt tgtatccggt ttgccgttgt gtcctgtgat
     1021 gatgattttg ggcgcggttt ttctgttttg atgtgtgaaa tgccgtctga aaggcggttc
     1081 agacggcata gcggtcattt ttgtgcggtc aggcggtcga atatgccgcc gtcggcgaag
     1141 taggttttca tgatgttgtc ccatccgccg aatttttctt cgggagagaa ggtgtctaag
     1201 tctgggaagt cggctttgtg tcttgccaat acttcggggt tgcgggggcg caggtagagt
     1261 gaggcggcga gttcttgcgc cggttcgctc caaaggtatt cgagataggc gcgggcggtt
     1321 ttttgcgtgc cttttttcgc gacgacgctg ttgacgacgg cgacggggct ttcggcggaa
     1381 atggtgtagc tcggatagac gatttcaaac tgtccttggg tcagtttttt gctgacgtag
     1441 ttggcttcgt tttcaaaagt gatgagtacg tcgccgatgt tgcgttgtgt gaaggtggtg
     1501 gtggcggcgc gtccgccgtt ttcaaaaacg ggggtgtttt tgaggatgga tgcgacgagt
     1561 ttttgggctt cctgttcgtt gccgttggtg gttttcagac cgtaaccgta tgcgccgagg
     1621 aaggcgtagc gtccgttgcc cgaggttttg ggattggcga tgacgatgtt aacgccgtct
     1681 ttggcaaggt cgttccaatc gcggatctgt ttggggttgt tttttcggac aaggaaaacc
     1741 atagtgctgg tgtagggcgc ggcgtggtcg gggagggctt gttgccagcc tttttctacc
     1801 agtccttttt tttcgagcag gtcgatgtcg gaggattggt tcatggttac gacatcggct
     1861 tgaaggccgt tggctacgga taatgcctgt ttgctggagc cgccgtggga ctgttggatg
     1921 ctgacggatg tgccggggtg ttcggattgg tatgttttga taaataaggg gttgtattct
     1981 ttgtaaaaat cccgtgccac atcgtatgag gcgttgagca gggtaatgtt ttttccgtcg
     2041 gattcggtat tggccggggc attttgtccg gacggatggt ttgaatcggc tgcggggctg
     2101 caggcggtga gcagggctgc ggtatagagt gccggtgcgt aggttttcat atgcttgtcc
     2161 tgtcggttgg tagatggggc aactttatac ggctgtctgc gcttgtggaa ataatgtttg
     2221 atttgaagat tatcagtttt ggttataagg acggatcaga ggtgtttccg catcagttcg
     2281 catttgattt tgatgctggg gtcaagctgc aatactgccg aaccgagcga ttcgtagcgg
     2341 ttgagaaggt aaaacgggac ggagttgagc gatgcgtaga gtttcagaaa gctcaaaccg
     2401 gatttgtggg cgatggtttc cgcctgatga agcagggcag tgcccagtcc gaggttgtgg
     2461 aacagggggt ggacgtaaag tgcatcgagt tgtgcttctt ggcagtcgat ttggaaaaat
     2521 ccctgtatgt tgcctttgta ttcggcaacc caaagtgctt tgtcgggatc ggaaatggtc
     2581 ggcaggtagc tttctgtgtt taacaagcct tcccatactt ttagggcgtg ttcgttgtag
     2641 ctgaggatgc aggtgtattg gacggagtgc aggtggactt tgaagatgtc tttgcagtct
     2701 tgcacggtgg cggggcgtaa cagtgtcagc aggttcatgg cagtatgtcg gcggcttcag
     2761 acggcatctg tgccgttggt cggattatag ggactgatgc agtttttttg cttcttgaaa
     2821 tgcggtgtcc gaatcggtgc ttaaaacggt aaagtgtccc attttccgcc ctttgtgcgc
     2881 ggtttttttg ccgtaaaggt gcaggtgcgc gtccggccgg ctttgcaggg gaaaccaatc
     2941 cggttcgccg ccgtcttcct gccaaacgtc gcccaaaata ttcgccatac agcaagaact
     3001 cagcaatttg gtgtcagcag gtggcaggtt gcacatcagg cggacctgtt gctggaattg
     3061 gtctgccgcg caggcgtcga cggtatggtg gccggaattg tgcggacgcg gcgcgatttc
     3121 gttgacgacc aattcatgcg tgtcgccgac aacaaacatt tctaccgcca atacgccgac
     3181 gtagttcaat tcatcggcca aacgctgcgc catttgtcgc gcctgttgct gaatgtcggc
     3241 actcagtcgg gctgggacga tggagtaggc gaggataccg ttttcgtgaa tgttttcggc
     3301 aggatcgaaa gtttgcacgt tgtcattgtt cagacggcat acgataacgg aaatttcgcc
     3361 gcgcaagtcc accatttttt ccaaaacgca atccacgccg cggtgttcgg caaacgcggc
     3421 tttgagttca tccaccgttt tgacgcggat ttggcctttg ccgtcatagc ccaatgtagc
     3481 ggttttcagg atgccgggca gaaattgtat gctttcttca gtgatgtctt cggctttgca
     3541 aattgcttga tacggcgcgg tttgcaggcc tgctttgcgt atccatgcct tttcctgaat
     3601 gcggttttgc gcgatggcaa cgcagtcgcc gctgggggaa acattggtat gtttggcgag
     3661 aaaacgcatc gcatcggcat tgacgttttc gaactcggtc gtaacagccg cacattttgc
     3721 caattcttcc aaagcggttt ggttgtcaaa cggcgcacac aaatggcggt cggcaaattc
     3781 cgctgccggc gcattcgggt tgggatcgag tacggttact ttgtagccca tggttttggc
     3841 agcaacagta aacattctgc ctaattgtcc gccgccaaga atgccgagca tggcgggcgg
     3901 agaaagagat atgtttttca tgctgactct tcaaattgta caagttgata gctataacta
     3961 attcttgacg gatgtcttgt atcgctggaa ttaccagttt cagaaataca gaatactttt
     4021 tccataaatt tttctgcttt tagaaattcc agtattctgt ttttttcatc cttataagca
     4081 ctgcggtctg taccccatgc aagaataatc atatcagcat cttccaaaca tcctttaaat
     4141 ttggaaaaat cggtttgggt gtttgcccta attcctgttt gctgtgtaga gtaactggaa
     4201 aaaatattta acattttgaa gttggtaaaa ccgtacatat ccaagaaacg tgcaagttgg
     4261 gtaagggttt tgtcgcttct ttcatcattt gctttacttg gattaattcc tatagcaaca
     4321 gctgagaaat ttttaggatt ttctgtgttg ccgctccatc taaccgttag gatttctcga
     4381 cttttttcat tatctgtata gagaccgtct ttggtagtcg gtctgagtgt ttggcgaagc
     4441 tcataaagtt tttcataagt catttatcca acccttcctg caccatctgc gcggcgcgta
     4501 taacggctcg ggctttgttt tgtgtttcct gccattcgga ggccggatcg gagtcggcaa
     4561 ccacgcccgc gccgctttgg acgtatagcg tgttgttttt tactacggcg gtgcggatgg
     4621 cgattgccaa atccatgtcg ttgttgaaac cccatacgcc gacggcaccg ccgtagatgc
     4681 cgcgtttgct cggttcgact tcttcgatga tttccatggc gcggactttg ggtgcgccgg
     4741 agagtgtgcc ggcagggaag gtagcggcga ggatgtccat gttggtcatg ccgtctttca
     4801 gacggccttc gacgttggaa acgatgtgca ttacatggga gtatttttca atcaccattt
     4861 tgtcggtaac tttgacttcg ccggttttac tgatgcggcc gacgtcgttg cgtcctaagt
     4921 caatcaacat gacgtgttcg gcgatttctt tggcatcgct taacaaatct tgttcgttgg
     4981 caaggtcttc ggcgggggtt ttgccgcgca ggcgcgtgcc ggcgatgggg cggacgatga
     5041 catcgtcgcg ttcgcggcgg acgaggattt cgggcgagga gccgacgatg tggaaatcgc
     5101 cgaaatcgta gtaaaagaga taaggcgaag ggttgagcgt acgcagggcg cggtagaggg
     5161 cgagcgggct gtcggtgaat tccatgctca tgcgctggct ggggacaacc tgcatgcagt
     5221 cgcctgcgaa gatgtagtct ttgattttgt taacgcaggc tttgaacggc tcttcgccga
     5281 attcgctgac ggcttcggtg tgtttgctgc cgagcgagag cgggatggcg cagctttggc
     5341 gcaactgggt gcggatgtct tcgaggcgtt cgcgggcgcg ttcgtagccg tcgggctgcg
     5401 acggatcggc gtaaacgacg agatggattt tgccgctcaa attgtcgatc accgccaatt
     5461 cttgcgacag catcagcaag atgtcgggcg tgccgagcgg gtcggctttg gtggtgtttt
     5521 tcaggcggtg ggcgaagtgt tcaaaattgt agatggtttc gtaaccgaag tagccgacca
     5581 gtccgccggt aaagcgcggc aggcttggga tttcgggtgt tttgaagcgg ttgtggaagg
     5641 cttcgataaa gggcaggggg ttgccgtcgt gttgctcgac aatttcgccg ttttgataaa
     5701 catcgacgtg tttgccgctg gctttgagat agtggctgca aggcaggccg ataaaagaat
     5761 agcggccgaa acgttcgcca ccgacaacgg attcgagcag gtaggtatag gggcggttgg
     5821 cgagtttgag atagagggaa agcggcgtat ccaaatcggc aaggagttct tgcacgagcg
     5881 ggatgcggtt gtagccttgg gcggcttggg cttggtattc ttgtttgctg atcatttctg
     5941 ctttcccaaa gggcggtttc ggacggcgcg gcaacgggcg cgagtatagc attttatcgg
     6001 aattgttgac agtctgaccg gagatgccgt ctgaaaagga cgggcttacg gctttgtttt
     6061 gcgctgccgg tattgtttcc atttaaacag ataacctgcg gttacgggaa tcagggctaa
     6121 gatggttttg aacgtccatg atgcatacca aggtaaagaa ccgggtgcga ccgattgatt
     6181 cagatacggc caccaacagg cagtcaggca gccgagcagc agcggccaga tatgccggct
     6241 gaggtttttt tgccaaagga ggaatgatgc ggcagaccaa agcagggtaa gggcagtgat
     6301 ttttaacacg gtataagcat ccgctccaaa cagggcggcg agaaaatacg taccggcaat
     6361 ccagaggacg gcaagtatca ggacaatgat ttcttcaggt cgtttgaaca tttttttctt
     6421 cctgtttgat ttcagacggc attgccgttc tgtttggttt ccagcagctc ccagcgttcc
     6481 agcttttcca aaagcagcat ttcgatttct tcggcgcggc tttgcagtgc gccggctttt
     6541 tcgtaatctt tgaagatttc gggatcggaa agctgcgcgt tgatttctgc ctgttcggct
     6601 tccaaggcgg cgatttcgtc gggcagggcg tcgagttcgc gctgttcttt gtaggacagt
     6661 ttgaccgtgc ggttggcttt gggtttttct ttcaccggtt cggcatcggc tgttttgggt
     6721 gcggattccg tctgaatctt cgcttcgcgt gattttgcgt cgatgtagtc ctgatagccg
     6781 ccgatatatt cttttaacct tccatttcct tcaaaaataa tgctttgggt aatcacgtta
     6841 tcgaggaaca tacggtcgtg tgagacaagg aataccgtgc cttgataatc gcgcagcaaa
     6901 tcttcaagca gctcttgggt atcgatatcc aagtcgttgg tcggttcgtc caaaatcaaa
     6961 atattagcag gacgggtaaa gagttttgcc agcaaaaggc ggttgcgttc gccgccggag
     7021 agcgatgaaa cgggactttg tgcgcgggct ggatggaata agaaatcttc cagatagctc
     7081 attacgtgtt ttttcttgcc gccgacttcg acgtaatcgt tgccttgacc gagcgtgtaa
     7141 aacacggtgt cgttttcgtt caacgtgctg cggaattggt cgaaataggc aacttcctgc
     7201 ttactgccga tacggattct gccgtaggtc ggctgcaatt cgcccaaaat cagcttgagg
     7261 aaggtggttt tgccgatacc gtttgggccg attaagccga ttttgtcgcc acgctgcaag
     7321 atggcggaga atttgtccat gatgactttg tcgccatagg taaacgaggc atgttccagt
     7381 tcggcaatga ttttgccgct tttctcgccg ctgtcgagct tgaaattgac ctgcccttgt
     7441 acgttgcggc gttcggcacg ctggcggcgc agttcttcca aacggcgcac gcggccttcg
     7501 ttgcgggtac ggcgcgcttc gatgcctttg cgtatccacg cttcttcctg cgcgtggaat
     7561 ttgtcgaaga ggcggttgtg ttccgcttcg actgccaact cttgcgcttt tttctcgcta
     7621 tatttggaga acgagccggg ataggaacgc aaaataccgc gatcgagttc gacaatccgc
     7681 gtggcgatat tgtccaaaaa acggcggtcg tgggtaatca caaccaggct gccttcaaac
     7741 gctttgagca ggttttccaa ccaaataatc gcgtcgatgt ccaaatggtt ggtcggttca
     7801 tcgagcagca atacgtcggg cttctgcacc caagcctgcg ccaaggcgac gcgctttttc
     7861 tgaccgccgg agaggttgcc gattttttcg ttttccggca aaccgagttc gcccaaagtc
     7921 tgcttcaccg ccgcatccaa cttccagccg tccttcgctt cgatctcaag ttgcaattcg
     7981 ttgagctctt tcaataaggc ctcactcgaa ccattttcca actcatggct gacatgatga
     8041 taacggcgca ataaatcgcg aatttcgccc aaaccttcgg caacagtatc aaatacggtt
     8101 gcttccttat caaaaaagga ttcctgcggt acataaacga ttttgaggtt gttttggaca
     8161 ataatctgcc catcatcaag cttttgcacg ccggcgagga tttttaaaaa cgaagactta
     8221 cccgcaccat tacgaccgat taagccgatt ttttcgcctc tgtcgagttg gaaggaagtt
     8281 ttgtcgagca gggcgacgtg gccgacggca aaggaagcgt tttctacaga taatatattc
     8341 atgatacaaa ttctcaacag ttaccgtttg gattttaccg caagtttggc gcgggcaatt
     8401 tcaaccgcac ccggcaggac ggaaacaata atgatgccgc ccatcaccaa gcccagattg
     8461 ttttttacga cggggaagtt ggcaaagaaa tagcccgcgt aagaaaacag gataacccac
     8521 aacaagccac cgatgatgtt gtagcggata aatttggcat agtgcatttt ccccataccg
     8581 gcgacgaagg gggcgaaggt gcggacgatg ggcataaaac gggcaatgat gatggttttg
     8641 ccgccgtgtt tttcgtaaaa acggtgggtt ttatcgagat attcacgtcg gaagatttta
     8701 gaatcgggat tggcgaacag cctgccgccg aaatatttgc cgacggtaaa attgagcgcg
     8761 tcgccgagta tggcggcaag gcttaataat gcaaccatca aatgaatatc cataccgccc
     8821 agcgcggcaa taccgccggc ggcaaacagc agcgaatcgc cgggcagtaa gggcgtaaca
     8881 atcaggccgg tttcgcaaaa aacaatcaaa aacagaatcg cataaatcca cacaccgtat
     8941 tgcgccgaca gcgcgagcag gtgttggtcg atatggagga tgaagtcgat ggcggaggca
     9001 agcacggcgc gttcctaaaa aaaacaaacc gcgtatttta accgattgga aaaatgccgt
     9061 ctgaaatttt cagacggcat cggctattca aattcatttc acgtaaaaac cgcaaaccaa
     9121 aatagtttgc ggtttggcat ttaaagtgac aatgatgatt tcaaatcatc agaattttat
     9181 gccgacgcgc aagccgtatt cacgaatact ggttttcggg atggtgagcg atacgtcgcc
     9241 actcttggtt gttacactaa actcgccgga ttctttgtaa gtgcgttgtt tgtagaacgg
     9301 ccccgcctcg atgctggcgg attcgcccag ttttttacca atatttgcac ccaatccaaa
     9361 gccccagccg ttggtttttt gattgatgtc ttttttgaga ttggtaacgc cggtgtttaa
     9421 tttatagcgg gaattgaggt caaatttcac ttcagaccaa gggttgatat accagccgtt
     9481 acccagttgg gaaagcaaat ccgcgtgaac tttggctaac cacgactgac ggctgctgtg
     9541 aagcgtatgc ttggtggttt taatgctgtc ttttgaagat tcaaaaccca agccggcacc
     9601 cacacggaaa tttaaagaat cacttaacgt ttgggtgtag gtgtagcctg tgtaaagatc
     9661 gatacggttt tcaggaacgc cggtgggcag ttttacattt ttagtcttac ccagcttgtt
     9721 ctcatctgtt tccaaattaa taatattttt tttgctgcgc ccgaaaccgg cttccaagcg
     9781 gatgccttgg ttggcatcaa aaggaatatc agcacgtacg ccgatgtgtt tggcagcttt
     9841 gtgtttttct ttcaggaaag cacgagttga agaaatggaa gagaggtcgg tgtggacggt
     9901 aaactcatta gcggtttgaa gctcttgtgc agcggcggca gtaccggtca gggcaatcat
     9961 ggcacatgta aaaactgttt ttttcatagt taaaatctcc aaaatttgga ttgtagtcgg
    10021 atatggtaac ataacgtaaa taatcgttac gcttacaatt atattcttaa gctttcgggg
    10081 gggggggatt ttacgtatat taataaaaat taacaaatag ttatttgttt acaacgaatt
    10141 gttattctca cttggttttc tgttttttat gggaatgacg aaattttagg tttctgattt
    10201 cggttttctg ttttaaggga atgacgagac ttgagatggc ggcatttatc gggagcaact
    10261 gaaaccactc tgccgtcatt cccgcgaaag cgggaatcta ggttcgtccg gtttcggtta
    10321 tttccgatag attcctgccg cgttgggggt ctggattccc gcctgcgcgg gaatgacgaa
    10381 tttcaggttg ctgtttttgg ttttctgttt ttgtgaaaat aacaggatga gatctttgca
    10441 aaattccttt ccctcccgac agccgaaacc caaacacagg ttttcgtcta ttttcgcccc
    10501 aaatacctcc taattttacc caaatacccc cttaaccctc cccggatacc cgataatcag
    10561 gcatccggtc tccttttagg cggcagcggg cgcacttagc ttgttggcgg ctttcaacag
    10621 gttcaaacac atcgccttca gatggctttg cgcactcact ttaatcagtc cgaaataggc
    10681 tgcccgcgc
//
LOCUS       DAWFIT010000066        10425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFIT010000066 DAWFIT010000000
VERSION     DAWFIT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.066"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..297)
                     /locus_tag="WGQ85_001576"
     CDS             complement(<1..297)
                     /locus_tag="WGQ85_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0750960.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGQ85_001577"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGQ85_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0750961.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGQ85_001578"
     CDS             complement(1660..4875)
                     /gene="carB"
                     /locus_tag="WGQ85_001578"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0750962.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VNTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4882..5430)
                     /locus_tag="WGQ85_001579"
     CDS             complement(4882..5430)
                     /locus_tag="WGQ85_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750963.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5433..5996)
                     /locus_tag="WGQ85_001580"
     CDS             complement(5433..5996)
                     /locus_tag="WGQ85_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0750964.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6848..7237)
                     /locus_tag="WGQ85_001581"
     CDS             complement(6848..7237)
                     /locus_tag="WGQ85_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0750965.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7810..7935)
                     /locus_tag="WGQ85_001582"
                     /pseudo
     CDS             complement(7810..7935)
                     /locus_tag="WGQ85_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGQ85_001583"
     CDS             complement(7981..9114)
                     /gene="carA"
                     /locus_tag="WGQ85_001583"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0750966.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2384 a         2677 c         2653 g         2711 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacggcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcctt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgtttcaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca atctccgctt
     1381 acgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg tcataaaact gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgaaaccgc
     1621 aaaacggttt cagacggcat tttcaacctg attcaggcat cagttgcggt ttttcaaacg
     1681 cccgtgcagc tcttgaacgc tgtacacgcc cagatggtct cggcttttcg cgccttcgct
     1741 catcgcttcg ccgccggcag tagtggtgta ttgcggcaca cgttgctgca atgcgctttg
     1801 gcggatgatg tggctgtcgg acacggattg cggatcgctg gaaacggtat tcacgaccag
     1861 tgcgatttcg ccgtttttca gcgcgtcgcc gatgtgcggg cggccttcgg ggactttgtt
     1921 gatggtctgc acaattagcc cgtgttcggt caggtattgc gccgtgccgc gcgtggcgca
     1981 gatgccgtag cctaaggctt ggaagttttt ggcggttttg atgacgcgtt ctttgtcttc
     2041 ttcgcgcacg gagaggaaga ttttgccggt cgggttgagg cgttcgcctg cgccgagttg
     2101 ggctttgtag taggcttcgc cgaagcttgc gcccacgccc atgacttcgc cggtggagcg
     2161 catttccggt cccaaaatcg tgtccacgcc cgggaatttg atgaatggga acacggcttc
     2221 tttaacggca tagaaatcgg ggacaacttc tttttccacg ccttgttctt tcagggaaat
     2281 acctgccatg cagcgtgcgc cgactttggc gagcggcacg ccggtggctt tggagacgaa
     2341 gggcacggta cggctggcgc gcgggttcac ttccaacacg aacaccacgc cgtcctgcac
     2401 ggcaaactgc acgttcatca gaccgaccac gcccagcgcg tacgccatcg ctttggtttg
     2461 gcggcggatt tcgtcttgga tttcttcgct taaggagtag ggcggcagcg agcagccaga
     2521 gtcgccggag tgaatgcctg cctgttcgac gtgctgcata atgccgccga taaccacgtc
     2581 tttgccgtct gaaacgcagt ccacatccac ttcaatcgcg ttgttgagga agaaatcgag
     2641 caacacgggg ctgtcttcgg aaacctgcac ggcttcgcgc atgtattttt gcaaggcttc
     2701 ggcagagtgg acaatctgca tcgcgcgtcc gcccaaaaca taagacgggc gcacgaccag
     2761 cggatagccg atttcttcgg ctttgacgag tgcttcttct tcgttgtggg cgatgcggtt
     2821 gggcggttgg cgcagaccta agtcgttcaa cactttttgg aagcgttcgc ggtcttcggc
     2881 ggcgtcgatg ctgtcggcgg acgtgccgat gatgtttacg ccgttttcaa ccaacgcgtt
     2941 ggcgagtttc agcggggttt gaccgccgta atgcacaatc acgccccacg ggttttcggt
     3001 gcggacgatt tccaacacgt cttccaatgt cagcggctcg aaatagaggc ggtcgctggt
     3061 gtcgaagtcg gtggacacgg tttctgggtt gcagttgacc atgatggttt caaagcccga
     3121 ttcgcgcagg gcgagtgcgg cgtgaacgca gcagtagtca aactcgatgc cctgaccgat
     3181 gcggttcggg ccgccgccga gaatcatcac ttttttacgg tcggaaggac gggattcgca
     3241 ttcctcttcg taagtggagt agaggtaggc ggtttcggtg gcaaactcgg cggcacaggt
     3301 gtcgacgcgt ttgtaaaccg gatgcagctt cagcgcgtag cggtgttcgc gaacttcttt
     3361 ttcgcttacg ttcaacaatt gtgccaaacg tttgtcggag aagcctttgc gtttcagacg
     3421 gcgtagggcg gcgaaatcca aatcactcaa aatgccgtct gaaaccgctt tttcttcctt
     3481 catcaagtct tcgatttgcg ccaggaacca agggtcgatg gcgcagattt cgtggatttc
     3541 ttccagcgtg aagcccgcgc ggaacgcgtc tgccacaaac agcatacgtt cggggccggg
     3601 gtttgccagt tcgcggcgga tttccgcttt gtcggagctt ctcggattga agccgcacaa
     3661 gcctgtttcc aagccgcgca gggctttttg gaagctttcc tgaatcgtgc ggcccatcgc
     3721 catcacttcg cccacggatt tcatctgcgt ggtcaggcgg tcgtctgcgg cagggaattt
     3781 ttcaaatgcg aaacgcggga ttttggttac cacatagtcg atggaaggct cgaacgacgc
     3841 gggggttttg ccgccggtga tgtcgttgcg caactcgtcc agcgtaaagc cgaccgccag
     3901 ttttgccgcc accttcgcaa tcgggaagcc cgtcgctttg gaagccagcg cggacgaacg
     3961 gctcacgcgc gggttcatct caatcacaat catctcgccg ttttcagggt tcaccgcaaa
     4021 ctgcacgttc gagccgcccg tgttcacgcc gatttcgcgc aataccgcca acgaagcgtt
     4081 gcgcatgatt tggtattctt tgtccgtcaa cgtttgcgcc ggcgcaaccg taatcgagtc
     4141 gcccgtatgc acgcccatcg ggtcgaagtt ttcaatcgag cagatgatga tgcagttgtc
     4201 gtttttatcg cgtacgactt ccatctcgta ctctttccag ccaaggacgg actgctcaat
     4261 cagcagctcg tgcgtgggcg aggcatcgaa accgcgttcg caaatcgcca aaaactcgtc
     4321 tttattgtag gcaatgccgc cgcccgaacc gcccatggtg aaagaaggac gaatcagggt
     4381 agggaagccg acctgttctt gcgccgccaa agcttcgttc atggtgtggc agacaaaaga
     4441 tttcgggcaa gataaaccga ttttttccat cgcctcctta aagcgaccgc ggtcttctgc
     4501 tttgtcgatg gcgtcttccg tcgcaccgat caactcgaca ttgtatttcg ccagcacgcc
     4561 gttgcgcgcc aaatccagcg cacagttcag cgcggtctga ccacccatcg tgggcagaat
     4621 cgcatcgggc cgctccttgg cgataatctt ctccaccgtc tgccacataa tcggctcgat
     4681 gtaggtaaca tccgccattt cagggtcggt catgatggtg gcggggttag aattcaccag
     4741 aatgacttta tagccttctt cacgcaaagc cttgcaggcc tgtgcgcccg aatagtcaaa
     4801 ttcgcaggcc tgaccgataa cgatagggcc ggcgccgatg ataaggatgg attttaggtc
     4861 ggtacgtttg ggcatgggtg gttactcttg aattaaagaa ggttgttttg ttacatagct
     4921 tttaagaaaa gtatttccct tctctaaaat tgaagctaaa acatcatctt ctatgttaga
     4981 taaaatattc tctaaatctg cgcttgattt aattttaaag aattcttttg tttgagaatg
     5041 gtatacaaga gaaggtttat ttagctgtaa aagttctgct atattattta atgaaccaat
     5101 actcttacca tcccataaaa taaagccata atcagcaatt tctgccattt tcttatcttt
     5161 tgctgtataa aatacccttc cagttccttt gctgtctact tggacaaact gccaattccc
     5221 cacattattt cggtaaattt tgccagaaaa gtaaatatgc acattagcat aatcttgttc
     5281 ttgtagaaat ttttggatag ctttatcagc cccattagca tctccaataa caatatcaaa
     5341 attattgcta agaatattgt taattctttc tcgtatttgt ggatttagtc gtgaaatgct
     5401 gcgtgaacca gaaaagaaaa tagtgcgcat ttttatcctc tagtctttgt cattgctaaa
     5461 acaattacag atctagcatt ttttgaaaac aatttttcag ttgatatttc caatgttgct
     5521 ccgctatcaa ataaatcatc aattactaaa atattcttat tggatagatc tatattgtca
     5581 attgtgatgg aattattcag tatttctagt ttttctgatt tttcttcaat atttttgagt
     5641 ggtgtgtgtg agctagattt tcttaaaata ggagaatatg gaatattcaa gcgatcactt
     5701 agttctttgg caattaattg aactggttga ttaatacgct ctgttgtaaa aggggcggga
     5761 acaattaaat ttatagattc taaaccacta aatttttgta aaatatagtc taccagcaaa
     5821 gaaacatttt gggtttgatt acgatatttt aattgataaa cccattctcc aatcacactt
     5881 ctcttgctat caaagtgagg atgccctaat tcatcataac ctaacaaaat acttttttgc
     5941 atatgatggt ctaaagcaaa tccttttgtc cagttaccat ttagttctat cgccatttca
     6001 ttgactcctt gtcaaaatta agtataacga gcaatcctag caagccgtag cccgcatgta
     6061 gggtatgtgc ggtacgcacg cacgcgttct tcattttccc tgtaacccca acccaccgca
     6121 tgcgcgcctt gcggcacaca ctctgccgac ggattcaaag gtcgtctgaa aaaacgctcg
     6181 cggcgtttgt tacatctgcc ttgcgataaa ggctgcctga aaaattccca ctgttgatat
     6241 ttcgggcatt ctttctcaaa ccgcctgcaa ccgcaaaatc cgctgcaacc tgccccctcc
     6301 cccgtggggg agggctgggg agagggcatt ccccgaattt cggcaacctt tcccaacaac
     6361 tcaaccaccg aaatacaagc cttgtggctt gttgccctct ctctagctct ctcccacggg
     6421 agagaggacg gagtggctgt agggctaagg gttctgcaag gaaacaggtt gtgcaggttg
     6481 ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc aagtcgcttt tcaaaccgcc
     6541 tgcaaccgca aaatctgctg aaacctactc cctcccccgt gggggagggt tggggagagg
     6601 gcattccccg aatttcggca acctttccca acaactcaac caccgaaata caagccttgt
     6661 ggcttgttgc cctctctcta actctctccc acgggagaga ggactgggcg gttgttgggg
     6721 ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc agacggcctt
     6781 tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa tcaaaatcag
     6841 ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg atttctgtca
     6901 gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg gtaaagccca
     6961 agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg tgctgccctc
     7021 cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata tagtttccca
     7081 tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg tgctgccaca
     7141 attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg cgcaagatgg
     7201 ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt gttgtcgtgc tgtttgctgc
     7261 cctctctcta actctctccc acggggagag aggacggggc ggttgttggg gttaaagggg
     7321 ctgtaaggaa aacaagtcgt tcaggccact ttttactttt cagacgacct gcaatcaacg
     7381 gttgtaaatc taagttgcct ttcaaatcgc ctgcaaccgc aaaatctgct gcaaccttct
     7441 ccctcccccg tgggggaggg ctggggagag ggcattcttc gaatttcggc aaccttcccc
     7501 aacaccctaa ccgcccaaac acaagccttg ctgtttgttg ccctctctct aacgctttcc
     7561 cacagggaga caggacgggg cggctgtagt gattaagggt tttgcaagga aaacaaggaa
     7621 aacaagttgt acaggctgtt ttttattttt caagcgacct gaaatcaacg cttgtaaatc
     7681 caagtcgctt ttcaaactgc ctgcaaccgc aaaatctgct gcaacctacc ccctcccccg
     7741 tgggggaggg ctggggagag ggcattttcc aagttgcggc aatctttccc aaatccctta
     7801 gtcttctaaa atacaagcct tacggttgtt gccaaatgaa acttcttcca tacccaaaac
     7861 ttgcgtgtaa aacgcaatgg ttcggtcaat gtcggcaacg gtcagtacca gatggtcgag
     7921 tgcgctaatc ttcatttccc gctttctttt tcaggcggcg gcagtgccgt ctgaaagcca
     7981 ttatgcccgt tttgccgctt tcatattgcc aatgaatttg tcaaacaaat agccgacatc
     8041 ttgcggaccg gggctggctt cggggtggcc ttggaagcag aacacaggtt tgtcggtcag
     8101 ctcgatgcct tgcaaagtgt tgtcaaacaa ggatttgtgg gtaatgcgtg cgttagcggg
     8161 cagggtgtcg gcatcaacgg caaaaccgtg gttttggctg gtgatgacga ctttgccgct
     8221 gtccaaatct tgcacagggt ggttcgcacc gtggtggctg aagcgcattt tcagggtttt
     8281 cgcgccgata gcgaggctga tgagctggtg tcccaagcag atgccgaaaa tcggtttgcc
     8341 gctttccatc agtttttgca cggcttcgat ggcgtaggtg caaggctcgg ggtcgccggg
     8401 gccgttggac aggaacacgc cgtcgggatt gagtgccaac acgtcttgtg cgctcgtttg
     8461 ggcggggacg acggtcaggc ggcagccgcg cgaggcgagc atacgcagga tgttggtttt
     8521 cacgccgaaa tcgtaggcga cgacgtggta aggctgttcg tcaggggtaa cgaaaccctt
     8581 gcccaattcc cattcgcctt ccgtccattc gtaagtttcc gtgcaggaaa cttcttttgc
     8641 caagtctttg ccgaccatac tgccgaacgc ggcgatgagt tcttgcgctt tttcgattgt
     8701 ggcatccgca ccggtcagaa tcgcgccgcc ttgcgcgcct ttttcgcgca acagcgtggt
     8761 caggcggcgg gtatcgatgt cggcgatggc gacggttttg ttgcgtacca gatagtcgtg
     8821 caggctttcg gaggcgcgga agttgctgtg caagagcggc aggtcgcgga taatcaggcc
     8881 ggaggcataa acgctgcggc tttcttcatc ttcggcgttg gtgccggtgt tgccgatgtg
     8941 tgggtaggtg agggtaacga tttgtttgca gtaggacggg tcggtcagga tttcctgata
     9001 gccagtcatc gaagtattga acacgacttc gccggaagtc gaaccttcgt aaccgattga
     9061 tgtgccgtgg aatacgctgc cgtcagcgag gacgaggagg gcgggggtgc tcatgatggg
     9121 aatcctgttt ttaataaaat tcttgacaat gccgtctgaa gggacttcgg cagggggcat
     9181 ccggtcggaa aaatccggtc aaaaaaaaac acgccgcgca agatagacgc gtaacgtgct
     9241 tttcgggcaa gtgcctatac cctgaaagca gcatatttta agacgaaaca tacccgattt
     9301 caagcgcaaa cgcggcaggc gggctgatat gttcgcgcga aaacggaaat ccgtggcgta
     9361 aaaccgcccg ctccgcgccc actatgcccg ttacgcattt ccccacgtcc gtccggcgaa
     9421 actatgggaa tacccgaatc gtcaggaata cctgaatcgt caggaatacc cgaatcgtca
     9481 ggaatacctg aatcgtcagg aatacctgaa tcgtcaggaa tacccgaatc gtcaggaata
     9541 cccgaatcgt caggaatacc cgaaccatca ttcccgcgca ggcgggaatc cagacctgtc
     9601 ggcacagaaa cttatcgggt ataacggttt cttgagattc cgcattctag attcccgcct
     9661 gcgcgggaat gacggcatcg gtttgacggt atttaattga attgcggaaa ttgacggatt
     9721 cagtgagatt ggcgggatga agcctaccct atagcccgcc ttttatagcg gattaacaaa
     9781 aaccggtacg ggttgccccg ccccggctca aagggaacga ttccctaagg cgcccaagca
     9841 ccaggcgaac cggttccgta ccatttgtac tgcctgcggc ccgccgcctt gtcctgattt
     9901 ttgttaatcc gctatacgaa cccgccccct cccgaaaaac gcaaaaaatg ccgtccgaaa
     9961 acctttcgga cggcattttc gcgtgaaccg tcattcccac gaaaacagaa aaccaaaatc
    10021 agaaacctaa aattcgtcat tcccacgaaa gtgggaatcc aggactcagg acctgagaaa
    10081 cctttttacc cgataagttc ccgtaccgac cggtccggat tcccgcctgc gcgggaatga
    10141 cgcgtttcat ttgcccccat cccgaaaacg caaacaatac cgtctgaaag ctctgtaggg
    10201 agggcttcag cccaccgatt ccatcaatcc cacatttccg ccgttttcac cgaatccgcc
    10261 ccttttcaca atccagtcct acgcaactga accgtcattc ccgcgcaggc gggaatccgg
    10321 acttgtcggc acggaaactt atcgggtaca acggtttctt gagattttac gtcctagatt
    10381 cccgcctgcg cgggaatgac ggcggagcgg tttctgtttt ttccg
//
LOCUS       DAWFIT010000067        10377 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFIT010000067 DAWFIT010000000
VERSION     DAWFIT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.067"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGQ85_001584"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGQ85_001584"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0750967.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIELVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECSCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WGQ85_001585"
     CDS             complement(1457..1846)
                     /locus_tag="WGQ85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0750968.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNKYFNVTLSKVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WGQ85_001586"
     CDS             2072..3250
                     /locus_tag="WGQ85_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0750969.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERAMGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WGQ85_001587"
     CDS             3316..5250
                     /locus_tag="WGQ85_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0750970.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKNISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5553..6335)
                     /locus_tag="WGQ85_001588"
     CDS             complement(5553..6335)
                     /locus_tag="WGQ85_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0750971.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6474..8663
                     /locus_tag="WGQ85_001589"
     CDS             6474..8663
                     /locus_tag="WGQ85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0750972.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPRAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8709..9782)
                     /locus_tag="WGQ85_001590"
     CDS             complement(8709..9782)
                     /locus_tag="WGQ85_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0750973.1"
                     /translation="MRPFFVGATVLAILGALVFFISPGAVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLSAQTAGFTSLAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVLLLFMAAVSRAFLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(9839..>10377)
                     /locus_tag="WGQ85_001591"
     CDS             complement(9839..>10377)
                     /locus_tag="WGQ85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0750974.1"
                     /translation="VDMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHT
                     HTHFDGKAQVAEQIKGIGSITTATLMAMLPELRRLSHKRIAGLAGIAPHPRESGETKF
                     KSRCFGGRSAVRKALHMATVAATRFEPLIRDFYQRLLSEGKPYKAAVTACMRKLLTIS
                     NARMRDYFAENDTAENGI"
BASE COUNT         2388 a         2702 c         3012 g         2275 t
ORIGIN      
        1 gacgtgttgc gataagggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtatcgcca
      121 cgtcaaatca gtggtgatga ccgcagctgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccaatt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tttcatcgtt gatgtcgatg gcgacaacgt
      601 gcgcgccgaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctc gaagcttgcg caggatccaa gccttctggg actttgaccg
      781 cgtaatcggc actcacgata cagtgggtcg ccatgccgcc gtcggcagta tagccagcgt
      841 tcaatacaga gcggcacaag gtttcgcggc cggtattgca gtattcgcaa gaaccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca tcttggaaag
     1321 gcacggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaaaatgc cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 ggtcggcctg aagggtaaag tggtcggggc tgactttgct taaggtaacg ttgaaatatt
     1621 tgttttgttt cagtttgttt ttgtcgtagg ttttaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcggcga cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaag cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgaccgctaa agaagatttg gaaagcgcac aggatgcgct
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggctcta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcagcagga aagcggcgaa cgcgccatgg gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaaaac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggctgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgagc tggcggcatt gcgccgcgaa cgcttcggtt tcatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggacaac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccataagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatt gccgccaacg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgctgctgac catgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaacgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ttcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tccgccatgt ccgtcatcgg cgcggtcgcc
     5161 tgctcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ttgaagctgc
     5281 aggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tctagattcc cacttgcgtg ggaatgacgg cggtgtgttt cgtattgttc agtttattat
     5401 tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg ctgtactggt ttaaatttaa
     5461 tccactataa ctttcagtgc ggattggttt tcagacggca tttcccgcaa aaaaggcttg
     5521 gaattttcca agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc
     5581 agttggggca tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc
     5641 gtgccgttga agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag
     5701 atgctgccgc cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg
     5761 tcgggctgac accataagat ttgcgccttg cgcgcggcat cggggtgcag gccggcaatg
     5821 ggcatcataa agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt
     5881 ttgcagaacg gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc
     5941 acttctttga tggctttgtc caaaggcagg gaggcgaagt cgattttgtt caaatcggcg
     6001 gcgcgttctt cggtcaggtt tttgcgcgtg tcgatgttga tgagttcgcc gacgaacata
     6061 tagccgcctt cggcatcggt gtagataatc tgcctgccgc tgacgacgac ttcgtaaatg
     6121 cctttgaccg gtgtttcgct gacgctcaac actttcaaat cttgggcgga ataggttttt
     6181 tccaaacgcg ctttcaaaga ggcggcaacg gatttgccgg cggactcggc tttgacggcg
     6241 ggttcggcgt tggcattgga aacgggcgtt tgcccgcaag ccagcagtgg gaggacggta
     6301 aagggggtca agattttgat taacttggtt ttcatataaa gatgattgcg cgtgttggaa
     6361 aagcggaatt gtatcaaatc tctgttgcgc ctgcattgcg cctaggctca atttatcgtc
     6421 tgaaaatagc ttccgcctgt taaaatacgc aaaaaaatga tttgcctgcc tgtatgatct
     6481 accaccgcat cgccgtaaac gtgccgcttt cagacggcct tttgacttat tcccattccg
     6541 atccgcttcc tccgggaacg cgggtgcttg tgcctttccg caataaaacc gtggtcggga
     6601 tggtgtggga aacggatatt gcgcccgata tggatacggc gcggattttg agcgttcgga
     6661 cggccttttc ggacgagccg ctgctgcccg aaagctggcg tgatttgttg gcgtttacgt
     6721 cgcgttatta ccactatccg acggggcagg cggtgtttgc cgcgctgccg cagggtttga
     6781 aggaaacgcg cgcggtggaa atgccgcagc cgccgttgtt ttacgcgctg aacgaagcgg
     6841 gcagggcgca aacgccgccg ctggcgcggt tcaacaaaaa agcggctctg tgggacgcac
     6901 tgctgtcggg cgggatgacg atggcggcgt tgaagcaggt aaacgcgcag gcggcgaaat
     6961 tgattgagga ttgggctgaa caaggctgga ttgaaacgac ggaagcggcg aagcccgttt
     7021 taaggtcgtg taatgggcag gcttcgcact ctgaatttgt attaaatgct gaccagcaga
     7081 aggcttccga tgccattcag acggcattgg gacgcttcca gccgtttctg ctgtacggca
     7141 tcaccggcag cggcaagacc gaggtgtatt ttgatgcgat ggcgaaagtg ttggctcagg
     7201 ggcggcaggt gttgtttctg ttgcccgaaa tcaacctcac gccgcagctt ttgaagcggg
     7261 tggaaaaccg ttttgccgac gtgccgaccg ccgtgttgca cagtcagatg gcggcaggca
     7321 agcgcacgca ggattatctg cgcgcgatgt tagggcaggc gaagctggtg atcggcacgc
     7381 ggctggcagt gttcacgccg atggatgatg tcgggctgat tgtggtcgat gaggaacacg
     7441 acggctcgtt caaacaagac aacgaattgc gctatcatgc ccgcgatttg gcggtgtggc
     7501 gggcgaagca gggcggctgc cccgtcgtgt tgggcagtgc cacgcccagc ttggagagct
     7561 ggcacaaggc gcaaagtggc gcgtaccgcc tgctgcaact gaccgaacgc gcccatgccg
     7621 ccgcgcaact gccgcaagtg gacattctca acgtaggccg tctgaaactc gacaacggct
     7681 tctcgccgca agccttgcag cttttgaaac agaactttga agcaggcggt atgtcgctgg
     7741 tgtaccttaa ccggcgcggc ttcgcgcccg cgctgttttg cggcgactgc ggtcatacct
     7801 tcggctgccc gaactgctcc gccaaaatgg tgctgcacca acgcgcccgc caactgcgct
     7861 gccaccactg cgaccaccgc gaacccatcc cgtacaaatg ccccgactgc ggcaaccaag
     7921 acctgaccgc cgtcggccac ggcacgcagc gcgtcgaaga aaccctgcgc gccttcctgc
     7981 ccagggccgc cgtcgtccgc gtcgacaggg acagcacggc gcacaaaaac gactgggcgg
     8041 atttgtaccg ccgcatcgcc gacaacgaaa tcgatattct ggtcggcacg cagatgctcg
     8101 ccaaaggtca tgatttcgcg cggctcaacc tcgttatcgt attgaacgcc gacggcagcc
     8161 tgtacagcgc ggatttccgc gcgccggaaa ggctgtttgc cgagctgatg caggtgtccg
     8221 gcagggcggg gcgcgccgac aagcccggca aggtgctgat acagacccaa ctgcccgaac
     8281 atcccgtctt cgccgccgtc aaagcgcagg actacgtcgt gtttgccgaa aacgaattga
     8341 acgaacggca aatgttcgcc atgccgccct tcggttttca gaccgccgtc cgcgcggacg
     8401 cgccgcgtgt tgccgatgcg ctggaatttc tcaatgccgc caaagaaacc ctcgccccgc
     8461 tgttgcccgc aagcgtttcc cagtttggcg ccgcccccat gctgatggtg cgcctcgccg
     8521 aacgcgaacg cgcgcaaatt ttcctcgaat ccccgtcccg acaggatttg caccgtgccg
     8581 tgagtttgtg ggtgcaggtg ttgcagcaaa acagggacgg caaaatcaga tggtcggtgg
     8641 atgtcgatcc gcaggaggct tgattatcgg caatacgatg ccgtctgaaa ccgtttcaga
     8701 cggcattttt attccggatc gtctgtaaac gcgttcgccc gaaagatcgg tacgaatgtc
     8761 agcaggtaaa gcacgaacac ggcggcggtc agaatcgcgg ggacggtgat gaagaatatt
     8821 ggattcacgt tcatcaagaa agcacgcgag acggcagcca taaagagcag gaggacggcg
     8881 atgcggcaga gtttcgggta gtcgagcttg gtaaagccgc tgtgccacag tccggcagtc
     8941 agccacacca tcatcacgct gcccatcatg ccaccgaggg taatcaggtg caggggcgcg
     9001 gaggcgggca ggttttgtaa tttcgccgcg cctgtccaca aatagcctgc ggcggcaaag
     9061 agttggagca ggtaataagt gcggacgtag tgtttgcgca ggagttcgtg atggtgaagc
     9121 tcacgcagct tggcaagcag gataaagccg acggcgagcg aggtaaaacc ggcggtttgc
     9181 gcagacagcc aaagttcggc ggcggcgtgc aggagcagga aggtaatggc gatgttttta
     9241 tagacgacat tggggatgaa tactgggtct ttcagacggc attctttcag ggcttccgcg
     9301 cccaaaagaa tactgacgcg cacggatacg aacatcaccg ccgccatatt tagatgcact
     9361 tgcgcgcgca acaggttcaa atcgccgctg acggcatatg ccgtctgaaa aacagtgaac
     9421 gcggcaagta acattagcag ggcgaagttg tcggtgtttc ggtctagcca aatcagccgg
     9481 gcgcagaaca gcagcaacac cagccaatag gcggcgacga aaaacgaggc agtttgcggc
     9541 gaaaagggca gtatagcgga tgcggcgagc aataatgccg ccatcaaagt cgcgacaggt
     9601 ttcaggttac ccgaaaaacc cgtccagtcc aacaaagccg cagtcaggaa accaccatat
     9661 gccgcaggta gcatgagttc taagaagatt tggcggtgca ggacgacggc accggggctg
     9721 atgaaaaaca ccaacgcgcc gagtatggca agcactgtcg cgccgacgaa aaacgggcgc
     9781 atagcaactg tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgttt
     9841 agataccgtt ttcggcggta tcgttttcgg caaaataatc acgcatccgg gcattcgata
     9901 tcgtcagcag tttgcgcata catgccgtaa cggcagcctt atacggctta ccctcggaca
     9961 gcaggcgttg gtagaaatcc cgaataagcg gttcaaaacg tgtcgctgcc acggtagcca
    10021 tatgcagtgc cttacgcacc gcagaccttc cgccaaagca gcggcttttg aatttggttt
    10081 ccccgctctc cctcgggtgc ggggcaatgc cggccaaacc cgctatccgt ttgtgcgaca
    10141 gccgcctcaa ttcgggtagc atcgccatca gcgtagccgt cgttatcgaa ccgatgcctt
    10201 tgatttgctc cgccacttgg gctttgccgt caaaatgcgt gtgggtgtgg tcgtcgattt
    10261 gtttgtccaa ttcgtcaatc agccggtcaa aatgggcaat cagttgtttg acgcttccga
    10321 cttgcgtttc gtgaacctga tgcagacggt ttttctcggc agtccgcata tccacca
//
LOCUS       DAWFIT010000068        10256 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFIT010000068 DAWFIT010000000
VERSION     DAWFIT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.068"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(27..350)
                     /locus_tag="WGQ85_001592"
     CDS             complement(27..350)
                     /locus_tag="WGQ85_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0750975.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(338..577)
                     /locus_tag="WGQ85_001593"
     CDS             complement(338..577)
                     /locus_tag="WGQ85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0750976.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(700..1497)
                     /gene="truA"
                     /locus_tag="WGQ85_001594"
     CDS             complement(700..1497)
                     /gene="truA"
                     /locus_tag="WGQ85_001594"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0750977.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRQAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2302..2487)
                     /locus_tag="WGQ85_001595"
     CDS             complement(2302..2487)
                     /locus_tag="WGQ85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750978.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(2598..3290)
                     /locus_tag="WGQ85_001596"
     CDS             complement(2598..3290)
                     /locus_tag="WGQ85_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0750979.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(3287..4030)
                     /locus_tag="WGQ85_001597"
     CDS             complement(3287..4030)
                     /locus_tag="WGQ85_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0750980.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGQ85_001598"
     CDS             complement(4059..4622)
                     /gene="gmhB"
                     /locus_tag="WGQ85_001598"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0750981.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            complement(4619..5191)
                     /locus_tag="WGQ85_001599"
     CDS             complement(4619..5191)
                     /locus_tag="WGQ85_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0750982.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGGLTNPTVPSKYTNVKKSSVR
                     IGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKIL
                     ELEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            5361..7013
                     /locus_tag="WGQ85_001600"
     CDS             5361..7013
                     /locus_tag="WGQ85_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0750983.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            complement(7083..8324)
                     /locus_tag="WGQ85_001601"
     CDS             complement(7083..8324)
                     /locus_tag="WGQ85_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0750984.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            8476..9204
                     /gene="ubiG"
                     /locus_tag="WGQ85_001602"
     CDS             8476..9204
                     /gene="ubiG"
                     /locus_tag="WGQ85_001602"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0750985.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            9241..10158
                     /gene="thrB"
                     /locus_tag="WGQ85_001603"
     CDS             9241..10158
                     /gene="thrB"
                     /locus_tag="WGQ85_001603"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0750986.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
BASE COUNT         2184 a         2417 c         3078 g         2577 t
ORIGIN      
        1 gaacacaatc cattcagact attcaatcag gcaaacatct cctgcaatac tgcaaacagt
       61 ttttcagccg tactgttgtc taaatttcca agatgtttga ccaatccggc tttatccaca
      121 gccctaatct gttcgggcaa aagcaaaccg tctttatcct gaaagcggac attgacgcgg
      181 aacggggcag gacggcttcc gctcgtcata ggaacgatca gcacagtctt gagatagttg
      241 tgtatttcag gaggagatac tacgacacaa ggacgtgtct ttttgatttc gcttcctaca
      301 gtcgggtcta aggagaccag atagattccg ccgcgtacta ccatatccat tctttatccg
      361 cttcgttttc aatttcggaa aaaaaatgct cctgctcggt ttcgacaagc attgcggcag
      421 cttctgccca tcctctgcga acggtaggac agcttaaaat aatattgccc ttttcaactg
      481 taacagccaa gctgtccact gcccctattt gacccaataa tgatttgggc agaatcactc
      541 cttgcgagtt tcccattttg cgtatgttga gaatcatatg cgtaccatat ttaccagttt
      601 atgtaataac aatgttatta ccttgatgag gtagtgtcaa tattgaaaaa gattgcctga
      661 cagtttgtcc gatttcaaaa tctccgcgac aagaatgttt taaagccatt cggggatttg
      721 ggggcggatg atgccgtatg cctcgggata gtcgacgccg gtcaggtaaa gcccgtcggg
      781 catgaaggtc ggtggggctt tgaggcggct gcgttcttga atgagggcgg cgaagccttc
      841 gacgctgagc ctgccgctgc cgacataaac gagcgcgccc atgatgttgc gtaccatatg
      901 gtgcaaaaag gcgttgccgt gcaaatcgag gcggacgagt cctgcgcttt gggtaaggtc
      961 ggcgcggtaa atggttttga cgggggattt tgcttggcac tcggcggcgc ggaagctgga
     1021 gaagtcttgt tcgccaatca aataggcggc agcctgccgc atcggttcga tgtcgagttt
     1081 gaggtgtgtc cagcctgccc tgtttttgag caggggggaa cggacggggg cggattcgag
     1141 caggtagcgg tagtgccgtc cgtatgcgtc aaatcgtgca tgaaattcgg gggcgacctg
     1201 tcgggcgtgc aaaacggcaa tgccttcggg caggtgggca tttacgccgc gtatccatgc
     1261 ctgttgggga cgggcggcag ttgtgtcgaa gtggacgact tgggcggtgg catgcacgcc
     1321 ggtgtcggtc ctgccggcaa cggtggtgga aaccgcttcc cctgctattt gggcgagcgc
     1381 ggtttccaat gccgcctgaa cggtcggtac gccgtcagcc tgtttctgcc agccgtaaaa
     1441 gcggctgccg tcataggata gggttattgc ccagcgttgt ttttgtgcgg tatccatcgg
     1501 atttgggatt cggataaatg ttcagactgc attgtatcgc agattttgca gggaaacggc
     1561 aaacgcccag ggcgagcggc gttgtttggg gagttgttgg ggggggggat gcagttgcta
     1621 cgaatcgcta tcctgtgaat tcaccctgtc aggagtgccc gaatcgtcat tcccgcgcag
     1681 gcgggaatct aggacgtaaa atctaaagaa accattttat agtggattaa caaaaaccag
     1741 tacagcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcatcaag
     1801 tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     1861 taatccacta tatccgataa gtttccgcac cgacaaaact agattcccac tttcgtggga
     1921 atgacgggat gcaggttcgt gggaatgacg cgaacagaaa cctcaaatcc cgtcattccc
     1981 gcgcaggcgg gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtct
     2041 gggattctgg attcccactt tcgtgggaat gacgggatgt aggttcgtgg gaatgacgcg
     2101 gtgcaggttt tcttaacccc gcgttctaga ttcccacttt cgtaggaatg acggcggtag
     2161 atttggcaga tgcggcggat tcggcaggtc tcaacccatc ctacaatcca ccctgaccgc
     2221 cttttacgaa tccgccgcca tcctgggaaa cgcaaaaaaa tgccgtccga aaacctttca
     2281 gacggcattt tcgcgggcaa atcagtaaaa gacttcgcgc cagcttaagc gtttcatacc
     2341 gcacgtcgga ccgggcgggt ttcgggtttc ggaagagcgg tgtgaaatga aacgcggcag
     2401 acggcgcgtg tgccgccatc ctgccccgaa ccggcggcaa agcgtcatcg cggtttgaat
     2461 cgggcgggcg cgtgcctgtt ccggcacgga tgccgtctga aagcggcggc agcggtatgt
     2521 gtcgccggct gtgatgctgc tttccggatg tccgtttggg aggaggtctg aattggcgga
     2581 tgcctgcccg ggccgcgtca gcctaaggcg aaaagttccc tgccgtggat tttgagccac
     2641 tgtttcgctt cgggcgtgta gggtgcgaag cggcgggtca gtgcccagaa cgcgcggctg
     2701 tggtcggcgt gggcgaggtg gcagagttcg tgtatgcaga catagtcggc aacgtattcc
     2761 ggtgcgccga ccagccgcca gttgaagcgt atgcctgtgg ttttgcggca cacgccccag
     2821 aaggttttgg cagaggtcag cgaggagggg gcggggaaca gttgtgtggt gcgggcgtgg
     2881 cgttcgaggc ggggaatcag gtaactgtgc gcctgccgtt ccaaaaagtc ccgcagcagc
     2941 gcaagctgtt tttcgggtgc gccttcggga acacggattt cagacggcat cagcaggatt
     3001 tgcgtgtctt gatgggcggt gagggcaagc tgtctgccgt ggaagaggat ggattcgggc
     3061 agccggtttt cggcagtttg cggcggcggt gttttcgcca gtgtttgccg caggacggct
     3121 tcgttttcat acagccagcg gtttagagcg gagacggaga agcagggtgg gacgctgatg
     3181 cggacggtat gtgtgccggc ggggcggata atcaggtttt tcttggcacg gcgcttgatt
     3241 tcgacggtca gttccatgcc gtctgaaagg gtgtggacaa aggcggtcat gcggtttcag
     3301 acggcatttt ggcggcaaac gggcctgcgc cggaaatgag cggctgctgc gtttcgatga
     3361 ggtgttcgca ttttcccatc aattcggctt cgctgccgct tgcgtgcggg atggtcggac
     3421 agatgacgac ggtgatttcc cccggatatt tcagaaagga gtttttcggc caaaattcgc
     3481 cgctgttgag ggcgacgggg acgatgtcca tctcaaacat tttcgccatg cgcgcgccgc
     3541 cgagtttgta tttgccgcgt tttccgggcg caaggcgtgt gccttcgggg aaaatggtaa
     3601 tccaatagcc ttcgtttttg cgcgccaacc cctgttttat gagctgctcg ttggcttcgc
     3661 ggcggttgtt gcggtctatg cctatggttt tgaccagttt caagccccag ccgaaaaagg
     3721 ggattttgaa caactcgcgt ttggcaacgt aaacctgcgg cggaaaaatg tcctgaaggg
     3781 cgagcgtttc ccagccgctt tggtgtttgg cgcagatgac ggcggggcgg tcggggatgt
     3841 tttccgcgcc gatgatgcgg tatttgagcc cgacgatgtg tttgagcgag aggttgagga
     3901 ttttgaccca gacccgcgcc atcttgtgcg ccccgtctcg gaaaggcgag gcgagcagca
     3961 taaagggaaa gaggaaaatc agggtggaac agagtatcag ccagtaaatc aggttgcgga
     4021 tgatgagcat ggttttgcct tgtcggaatg cggtatgttc agtcggcttg cggtgcggcg
     4081 ttttcctgca tgatgtattg tgagaaatcg agcaaggtat cgaaaacctg tgtgtgttcg
     4141 ggtaattcgt gtccgtgttg ggagagcgtt tttttgcctt ttccggtcag aaccagcgcg
     4201 ggttttccgc cgacggcatc gattgcctgc aaatcgcgca ggctgtcgcc gaccagccag
     4261 gtttccgagg cttgggcgtt gaagcgtccg atgatgtctt caatcatacc cggtttgggc
     4321 ttgcggcagt tgcagttgtc ggcatcggtg tgcgggcaga accagatgcc gttgatttcg
     4381 ccgcctgcct gacggacgag gcggtgcatt ttggcgtgca tttcggtaag gttttgaacg
     4441 gtaaaatatt tgcgcccgat gccggattgg ttggtggcaa cggcgacggt gtagcctgcc
     4501 tgcgtcagaa atgccaccgc atccatgctg ccttcgacag gtatccactc gtcaacggat
     4561 ttgacgaagt catcgcggtc ctgattgatg acgccgtcgc ggtcgagaat gatgagtttc
     4621 atcgcgtttc ctttgggttg gggcgtgtcg gggatgggat tataggacat aaatctagat
     4681 taaattctgc tacaagatgt ttctctaact ccaatatttt atcttgtgat ctgcgcttca
     4741 gtggtttaca atctaccccg acataaatag tccacggctc aagatttttt ctcactatag
     4801 cccctgctcc aacagcaacc ccatcaccaa tagtaacgcc tggtaatact gttacatttg
     4861 ttcctaaaat tgcatgtttt ccaattctaa cactagattt tttaacatta gtatatttag
     4921 atgggactgt tggattggtt aacccccccc cccccataaa atcatcgcta ccggtaataa
     4981 ttctacaccc tgcgcttaat cctgaaaaat gatccatata aaattctcca ccaccaataa
     5041 tactggtaaa tgatgatata tgaacaaaac ttcctaaaat acttttctct ccacaattaa
     5101 aaaatacaaa atcatcaatt tgagaataat ccccaattga taaattttct tttttaataa
     5161 tctttgcacc atcgtatatt ttataattca ctttttagtt cctctaaaag ggctcattgt
     5221 aacttcacca ttttcagtga ttccatctcg aataattata ctgaaatatc ggcagtgatg
     5281 cgcctgtgct gcattataat gcacgctttc gataataatt cgcaacggct caggtatgcc
     5341 gttttgagga gtggggcggg atggttgcct atgttttcct gtttttgttt gtaacggcgg
     5401 cgttggtgct gattatcagg tcgcactacc gctggacgta ttttttcgca tccgcgctgt
     5461 ttgtcttttt ggcgggcggt atgctgatgt tgacggctca gtggcaacgt gccttgaatt
     5521 tcgcttcggt ctggtttgtg gtgctgatac tgttccacag gctgaaaatc cattattaca
     5581 aacagccgct gttgatttcc gactttttgc tgattgccga ctggcggaat tgggaaacgc
     5641 tgttccatta taaggaagcg gttatcggta tggcggggct gctggctttg gcggcatatg
     5701 cggttttcgg ctggagcggt gcggattctt tggatgtgcc gtggcggtgg gcaggcgcgg
     5761 ttttgtttgc ggcggcgttc gtgtcgatgc ggcatttttc caaacatccg ggcgcggtaa
     5821 agacgtggct ggattcgctg ccggacgacg ggcgcgacgt gtttttgaac ctgccgatgt
     5881 cctgtcgggc ggtgtttttc caagtacccg tgttcgaggg cgacggggaa gcgtttgcca
     5941 ggcagatgcc gtctgaaacc cggccgtgcg gcatgtcgga tgaaaaaccc gatattgtcg
     6001 ttaccctgat ggaatcgacg ctcgatccgc actgtttcga ctttgccgcc gccaagattc
     6061 ccgatttgaa aatgttcgga cggcaggaag ataccgtatt ttcctcacct ttgcgcgtgc
     6121 atactttcgg cggcgcaacg tggaagtccg aatttgcgtt tttggcgggt gttccgtcca
     6181 cggatttcgg cgcgttggca agcggcgtgt tttactcggt cgtaccgcat ttgcagaccg
     6241 gttttgtccg caacctgcgc gaacacggtt atttttgcgt ggcactctcg ccatttacca
     6301 agggcaacta caatgccaag gcagcatatg accatttcgg ctttaatctg atgttccagc
     6361 cgcaagattt gggctatccc gcgccgatgg gcaaaaacct gtggcatatt tccagtgagg
     6421 aaatgatgca gtatgcgcgg atgattttgg aaaaacgcca tcccgatttg gaaaacgtgc
     6481 ggcagccgat gttcgtatat gtgctgacca tgaaggagca cgggccgtat cggacggata
     6541 ccgacaatgt gtttgatttg gatgcgcccg atttgaatgc gaaaaccgta tccgcgctta
     6601 acgactacat cgggcgcatt gccgatttgg acaaagcggt ggaaagtttc gaccgttatc
     6661 tgcacgaacg cggcaaaccc tttgttttcg gttatttcgg cgatcatcag gtaccgtttg
     6721 agggcgtgtc cgtcaggaag aaatgggatt acgcgcagcc ggattatgta acgcagtttg
     6781 ccgtcaggag caatattgcc ggcggattcg tacagcggca gaatttcctc gaccttgcct
     6841 ttgcaggcgg cgtgctgatg gaggcggctg gtctggaagc caaagacggc ttcatgcgtg
     6901 cgaatatggc gatgcgcggt ttgtgcggcg gagggttgga agactgcccg aactgggagt
     6961 tggtcggaaa ttaccgcaac tatctgtacg acgttttgaa aattgcccgt tagctttttg
     7021 ccgcatggct gcaacggttg cgaaaatgcc gtctgaaaac tcgttcagac ggcattttgc
     7081 ctttatcctt taaatacggg gacgagttcc atttggctca atacctgtgc ggcgatgttg
     7141 acgatgccga aaaggaagac ccaaaccatc agccacaagc cgccgtaaac tttataggtt
     7201 ttgcctgcgc cgaatttttt gcgcgaacgg tagagcagca tggcggggat gatgcctgtc
     7261 cagacggttg ccgccaggcc gacgtagccg atggcggtaa caaagccggt ggggaagagc
     7321 aggcaggaaa ttagaggcgg caggaaggtc agcgcggcgg ttttggtgcg gccggacacg
     7381 ctgtcgttcc atttgaagat gtcggcgatg tagtcgaaga gtccgagcgt tacgcctaaa
     7441 aacgaggtgg cgatcgccat ataggaaaac agggacaata ttttgtccat attgccggtt
     7501 tgggcgaatt tggacagggt ttcaatcagg acggagactt gcccttcggc ggcaatcacg
     7561 ggggcgaact cgttgcgcgg caggttgcct tggatggcgg tttgccagag gacgtaaatt
     7621 accagcgcaa tcagtgtgcc cgtccagatg gatttagcca ctttgggcgc gtcgccttta
     7681 aagtatttga gcaggctgga gacgttgccg tggaaaccga atgacgcaag gcagacgggc
     7741 agggcggtgg cgacataaat ccagtagttg gtgccggtag gggcttgggt gtcgaagagg
     7801 acgggcagtt tggcatcggc aatcaggccg ccggttgccc aaataaaggt taataccatg
     7861 ccgccgatga ggacgctggt gaatcggtcg accaagcgtg cggatgccca tacgcaaaag
     7921 gcgagaatgc cgaagaagac gagttgtccg acggtgagtg aaacattgcc gcctgccgcg
     7981 ctgcctaagc ctttggcggt caggtcgccg ccgacgaaga tataagcgta agtaagcagg
     8041 tataaaacga aggcgacggc gatgccgttg atgatgttcc agctccgtcc gagcaggtct
     8101 ttaaccatgg tgtcgaagct cgcgccgtgg gggtagtggg tgttgacttc caaaatcatc
     8161 aggccgctgg agagcatgga aaaccaggtg tacagcaaca cggccagcga gccggtaaac
     8221 catacgccgg atgtggcggt cgggttggcg agcatacctg cgccgatgac cgtgccggcg
     8281 ataatcatcg cgccgccgaa cagtgaaggg gttttggtgg gcatattttg tctttctgcc
     8341 agaaaaagcg agccgccatt atgccgtaaa gtgtaaggat ttgtaaggta tttgcgccgc
     8401 gccgcccgaa aaggctttca gacggcattg cgttccatag tataatcttg ggttttggag
     8461 tgggcggttc gtcagatggg agggaaaatg tccgacaaaa aatataatgt cgatgagggg
     8521 gaaatcgcca aattcagccg gattgccgac aaatggtggg acaagtcggg cgagttcaaa
     8581 accttgcacg acatcaatcc gctgcggctg gattatatcg acgggcacgc ggatttgtgc
     8641 ggcaaacggg ttttggacgt gggctgcggc ggcggcatct tggcggaaag tatggcacgg
     8701 cgcggcgcgg cgtttgtaaa gggtatcgac atggcggagc agtcgctgga aaccgcccgc
     8761 ctgcacgcgg ctttgaacaa tgtcgccgat atcgaatacg aatgtatccg cgtggaagac
     8821 cttgccgagg cggaaccgca ctcgttcgat gtggtaacgt gcatggaaat gatggaacac
     8881 gtccccgatc ccgccgccat cgtgcgtgct tgtgccaatc tggtcaaacc ggacggcatg
     8941 gtgttttttt ccaccatcaa taaaaacccg aaatcgtacc tgcatctgat tgtggcggcg
     9001 gaatatctgt tgaagtttgt ccccaaaggc acgcacgact ggaaaaaatt catcgcgcct
     9061 gccgagctgg cgcggatgtg ccgtcaggca ggcttggatg tggcggatac gaagggtatg
     9121 acttaccatg tgttgtcgca aacttatgcc ctgtgcgatt cgaccgatgt gaattatatg
     9181 tttgcctgcc gtccggcgtt ctgacggcgg gtttgcccgt ttttgagcaa gtgagttgat
     9241 atgtctgtct ataccagtgt ttccgatgat gaaatgcgcg gcttcctgag cggttacgat
     9301 ttgggtgaat ttgtttccct gcagggcatc gcgcagggga ttaccaacag caattatttt
     9361 ctgacgacga cttcgggacg ttatgtgctg accgtgtttg aagtgttgaa acaggaagag
     9421 ctgccgtttt ttctggagct taaccggcat ttgagtatga agggcgtggc ggttgccgcg
     9481 ccggttgcgc gcaaagacgg ccggcttgat tccgttttgg cgggcaagcc ggcttgtttg
     9541 gttgcctgcc tgaaaggttc ggataccgcg ctgccgacgg ctgagcagtg tttccatacc
     9601 ggcgcgatgt tggcgaaaat gcaccttgcc gccgccgatt tccctttgga aatggaaaac
     9661 ccgcgttaca atgcgtggtg gacggaggcg tgcgcccggc tgctgcccgt cctgtcgcaa
     9721 gacgatgccg cgctgctgtg ttccgaaatc gatgcgttaa aggacaatct cggcaatcat
     9781 ctgccttcgg gcatcatcca tgccgatttg tttaaagaca atgtgttgct tgacggcggt
     9841 caggtatcgg gtttcatcga tttctattat gcctgccggg gcaattttat gtatgacttg
     9901 gcgattgcgg tcaacgattg ggcaaggacg gcggacaata agttggatga ggcgttgaaa
     9961 aaggtgttta tcggcggtta tgagggcgtg cgccccttga gtgccgaaga aaaggcgtat
    10021 ttcccgaccg cccaacgtgc cggctgcatc cgtttttggg tgtcgcgcct gttggacttt
    10081 cattttccgc aggcgggcga gatgacgttt atcaaagacc cgaacgcgtt ccgcaacctg
    10141 ctgttgagtt tggattgagt gcgtccggcg tttgacagaa atgccgtctg aaagggtttc
    10201 agacggcatt tttatggctg attaaaacga aaatgagacg atagccgggt attttc
//
LOCUS       DAWFIT010000069        10239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFIT010000069 DAWFIT010000000
VERSION     DAWFIT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.069"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(65..1207)
                     /locus_tag="WGQ85_001604"
     CDS             complement(65..1207)
                     /locus_tag="WGQ85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0750987.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1256..1678)
                     /locus_tag="WGQ85_001605"
     CDS             complement(1256..1678)
                     /locus_tag="WGQ85_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0750988.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1671..2480)
                     /locus_tag="WGQ85_001606"
     CDS             complement(1671..2480)
                     /locus_tag="WGQ85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0750989.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2562..2939)
                     /locus_tag="WGQ85_001607"
     CDS             complement(2562..2939)
                     /locus_tag="WGQ85_001607"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0750990.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3139..3906)
                     /locus_tag="WGQ85_001608"
     CDS             complement(3139..3906)
                     /locus_tag="WGQ85_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0750991.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3916..4695)
                     /locus_tag="WGQ85_001609"
     CDS             complement(3916..4695)
                     /locus_tag="WGQ85_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0750992.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPT
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVKIIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4694..4801)
                     /locus_tag="WGQ85_001610"
                     /pseudo
     CDS             complement(4694..4801)
                     /locus_tag="WGQ85_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4847..5575)
                     /gene="pdxJ"
                     /locus_tag="WGQ85_001611"
     CDS             complement(4847..5575)
                     /gene="pdxJ"
                     /locus_tag="WGQ85_001611"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0750993.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5599..6390)
                     /gene="recO"
                     /locus_tag="WGQ85_001612"
     CDS             complement(5599..6390)
                     /gene="recO"
                     /locus_tag="WGQ85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0750994.1"
                     /translation="MSEHRINHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGQ85_001613"
     CDS             complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGQ85_001613"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0750995.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACLTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7590..8822)
                     /locus_tag="WGQ85_001614"
     CDS             complement(7590..8822)
                     /locus_tag="WGQ85_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0750996.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9342..10196)
                     /locus_tag="WGQ85_001615"
     CDS             complement(9342..10196)
                     /locus_tag="WGQ85_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0750997.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2423 a         2760 c         2686 g         2370 t
ORIGIN      
        1 tatcggccgg atattaaaaa aagccgtctg aacatttttc agacggcatt tttgtacact
       61 ttcctcaacg gttcgatccc tgcaccatat caatcaggaa caggcggcaa tcgaggttgc
      121 cgttataaag cggaattttg cgttttggcg acaggcgcat gaatttgggc atttccctgt
      181 cgccggtaaa catgccgacc cgccagccgg catagtgttt tttcaaccac gtacccaact
      241 gcggataaag cgcctgcaaa gcgtggactt cctcaaggcg cacgccgtag ggcggattgg
      301 acaccataat gccgttttcg ccgttcggtc ggacggactg cgcgtcggca acgccgaagg
      361 aaacgatgtc gtccacttcg gcactgcgtg cgttgtccaa tgccgtctga acgatgcggc
      421 ggtcgctgtc gctgcctgca atcggggcgc ggacggggcg ggtttgcgct tcggcgcggc
      481 ggcgcaaatc cgcccacagc gttttatcga aattttgcag tttttcaaaa ccgaagcggc
      541 gcatcatgcc cggtgcgcgg cctgtggcaa tccaagcggc ttcgatagca atcgtgccgc
      601 tgccgcaaaa cgggtcttga aacggctgcg tgccgtcgta gcctgccgag agcagcagtc
      661 cggcggcaag gttttcgcgc agcggggctt cgccggtatc caggcggtag ccgcgtttga
      721 acagggcttc gcccgaagtg tcgataaaga tttcgacatt gcgttcgttc aaaaaggcgt
      781 ggatgcggac atcgggcgcg gctttgtcca cgctcggacg tgcgtcgtaa atgtcgcgga
      841 aagcgtcgca gacggcatct ttgacggtca gtccgacaaa ttggaggctt ttgacgttgg
      901 cacgctttgc ctcgattttg actttgaacg tctgctgcaa ggtaaaccag ttgaaccagt
      961 tgatgttttt ggcaagtttg taaatgtcgc gctcggttcg gtatgtgcct ttggtcaggc
     1021 gtagcaggat gcggccggcg gtacgcgagt gcaggttggc ggcgtaaacc tgttccaatc
     1081 cgccccggca ggaaacgccg ccgtcaaacg cttgaacatc ggtacagccg aggctttcga
     1141 gttcttgcga taaaacggtt tccaagccgc gcgggcaggt ggcgaaaagt gtatataaag
     1201 tattcatagc gtgtcctttc cggacggatg ttccggcaat ggcggattga aaggtttatt
     1261 cggcggtaaa tgccgtctga aatgtccggc tgccgattgt aatgtccgcc gtaaaatcgc
     1321 gcctgccttc gacacagatg ggcaggcgga tgcgtactgc ctgccaagtc cccgacggtt
     1381 gccgctcgaa catatagcgg ttgaaaccca tatccatatt tttcatactg aagctgatgc
     1441 tgacctgttc cgtgccgacg ggcgcgtgtt cgatataaat atcaaacggt tttttggttg
     1501 aaacggcggc ggcgcggacg cggcttccgt ccggcagcgt gcaaccctcg gtcaaatcgc
     1561 attgcgccgc cacggcttgc ggctgctgcg cctgccacca ttgcaacaaa acgagcttga
     1621 cggcggcaaa ggcaatcagc agcagtgcgg caagcagtaa tttacggttt ttattcatga
     1681 ttcttcccta ttgtttcatc ccttaccacc acgtgcgccc ccaatccgag ccatagtttt
     1741 ccataacgtg caaccgtctg tccgtttgct gcaagtacga gcggcacgga tacgccctgc
     1801 cgcagggctt ctgccgcctc ttgtgcctga acatcatcgc acacgcgcca aacgaccgca
     1861 tccgcgccct tttcctgcgc ccgccgccat tgttcgcggg tctgcaccac catccacacg
     1921 ctgtcggcgt gttcgggtct gtcctgccat tgcgccgcct ccatcaaaac gttcgcaccg
     1981 cttccctctg ccgaacccaa aggcaggacg cggtacgcgc ccatactgtt ttctccgtgc
     2041 aaacccgttt tcaggctgcc gtacaaacgg cgcgggacgg acagcgaacg caaaagcgcg
     2101 ccgttggcgg gcagcatggg ggcaacggta aaatcacccg ccttctgcca agaccattcc
     2161 tgcccttcgc gggattgcgg tttgcccgtc cattggtcgg ggttgaccca taggaatttc
     2221 aggcagacgc gggcgtgttc gtaggaatgg attttggtca gccaaggcgt ggcggcgagg
     2281 atgcggatgc cgagttcttc ttcaaactcg cgttgcaagg cttggaagtc ggtttcgccc
     2341 gcttcgacct tgccgccggc aaattcccaa tatccggcat agggtttgcc ttcggggcgc
     2401 gagctgagca ggtagttgcc gtctgaatcg agcaggatgc cggcaacgac gcggataagg
     2461 ggtcgggtgt cttgaatcat ggcgggtacg cggttggtcg gtcggagtgc aaatgaggga
     2521 cgggcaatgc cgtcaacggg tttgccgcat ttcagacggc attatttttc ggcaacgaca
     2581 aacgccaata cggtgtcttc ttcgtcgctc atgctgaggc tgacgcggct gatgccttgt
     2641 tcctccagcc atttggacag ggcggggccg tagaaaaatt cgggcttgcc caatgcgtca
     2701 tgcccgatgc cgatgttgcg gaaggaaacc gcgccgcgta tgcccgtgcc gacggctttg
     2761 gcaaaggctt ctttggcggc aaagcgtttg gcgaggtagt tgacgggttt gcccgcttgc
     2821 ggaaattcaa gcagctcttc cggagtgagg atgcgcccgg caaacgcctg tccgaatttt
     2881 ttgtttaagc ggatgatgcg cttgagggaa acaatgtctg tgccgatgcc gtaaatcata
     2941 tttgcgctcc ttcgcccttg gttgcgggta gtgatgaatg tgatgatgaa tgccgtcaat
     3001 ttcttggggc gttaaaatgg cgtgaatatc cacttttggg gttttgtcgg taacaatttt
     3061 cactgtaacc ttttgcattt gaaattctaa aacatcgtgc aaattattta ataaggagtt
     3121 aaggcattta ataatgaatt aaagtttgat taaacccgaa tgcccgattt tcagagccaa
     3181 taccaataaa acaataaaaa ttaacatttt aaaaaaattt gggctaatct tagtccttaa
     3241 ccgaattcca acatacaatc caataacaga caatacggac agtaaaaata ttaaaccgta
     3301 ttcactctta tttaataacc aatactggtc tcttagcata tatatttgaa caattttcgc
     3361 caaaagatag catagattgc ttgattttac gatacgattt ttattttctg tttcgctaag
     3421 caaaaatatt aacaatatgg gagacatggc attggttgaa ccgccgatga tgcctgccaa
     3481 aaacccaaaa agaaccatat tcttattatt ggcaactact tgaatatttt ttgcttttgc
     3541 acatacattt aaaataccat tgacagaata atacaatgta atgattgcca tcagtaaaag
     3601 cagccaagac actggaagta tcaaaagcaa cttcacccca aaaatgctgc caacgacgct
     3661 gccgatagca agcaatttat aggtttttaa ataataaaca atctcttgcc aaaaaccttt
     3721 tttgttattg ctgcatagaa ccaacaagct cattaacagg cttggtaatg ccaccaaggc
     3781 aacaacctta gacaatggca tgttaaaagc caatgcggtt gtaccgagca tcggaaatcc
     3841 catgcctgta attccgtgca gtattgcggc agcaacaaaa acgatagatt gcattatttc
     3901 ttgcataacc gtcctttata cagtaattct tgctctaata taatgcctgt ttttatccgt
     3961 ataatcagtt ccggcatgca agccattgac attgtcaata ataatcaaac catcatcttg
     4021 tgcagaaaat tcttgaatat cactcgcatt ttttataatc tttacaagat aatcaagtgc
     4081 aagtaccatt tcctttgata ctttatttct attttcttca atacctttat agatacaatc
     4141 acttctaaat ctaatcattt gagtattgac cgataagata ttaccccatc tcacaccttg
     4201 ttttttatca aatgatgttg gtgttttaaa tggatataaa ttgcccgtta atgtttttaa
     4261 gtgtttttta ccagtttctg ttttagataa ctgattgaca atatctgatg aacttaaaaa
     4321 tagggaatta cctccatcat tggctgattt tactacatac ataaccaaat aactttccgg
     4381 gttttcacta aaagatgaat cactatgaag tggacattct ccaacatctt ccgagaaagt
     4441 tatatcatta tttattacat agtcatcgcc gcgatattta atatcccaaa atatagattt
     4501 aaaaccatcg tctttgtggt ttgtcagcat tcctaattga gatagaaagt caaataattt
     4561 cttgtttctc cggctgtcat caagatggtt tagattaaga ccgcgaacaa tacaataccc
     4621 aatacgctgt actgtattgt gaatttcttc tatcaaacta ttattgtttg cagataaaat
     4681 atacttttga ttcatatagt gaattaaatt taaaccagta cggcgttggc ttgccttgcc
     4741 gtactatttg tactgtctgc ggcttcgccg ccttgtcctg atttaaattt aatccactat
     4801 aacatatccc tgtcgtgaaa tgctgtctga aagggtttgc ctgcccttac ggcagcagcc
     4861 ttgccctgaa catcgcctcc ttcatctggc gcacggcttc gggcagtccg aggaagaggg
     4921 cttgggcaat cagcgaatgc ccgatgttca gttcgcggat ggcgaggatt tgggcgatgg
     4981 gggtaacgtt gtgtatggtc agtccgtgtc cggcgttgac gaccaagccc aaatcgccgg
     5041 caaaatgcgc gccgttttgc agacgctcga attgcttgag ttgttcggcg tggctgcgcg
     5101 cgtcggcata cgcgcctgtg tgcagctcga caacgggcgc gccgacatca taggcggctt
     5161 ggatttgcct gtcgtcggca tcgataaaga gagagactcg gatgcctgcg tcggtcagga
     5221 ttttggtgaa cccggcgatt ttttcctgtt gcgccaatac gtccaagccg ccttcggtcg
     5281 tgatttcctg acgtttttca ggcacgatgc acacgtcttc cggcatcact ttaagcgcgt
     5341 tttcgagcat ttcttccgtc aacgccattt caaggttcag gcgcgtgcgg atggcgtttt
     5401 tgacggcaaa cacatccgcg tctttgatgt ggcggcggtc ttcgcgcagg tgcatggtaa
     5461 tcaaatccgc accgtgcgtt tcggcaacca gtgccgcctc cacggggctg ggataagtcg
     5521 tgccgcgcgc attgcggacg gtagcgatgt ggtcgatgtt gacacctaaa agcataatct
     5581 ttccttttat ttctgccttc agacggcatt tgaagccgtg ccgtccgaag tcgggacggt
     5641 ttcccgggcg gtttctttgc ggtcaaactg ccgtatctgt tccaacacct gccgcgattt
     5701 cagcccctcg ggcaacaggt ggcggataaa aagccgtgtg attttcaatg cctgttgcag
     5761 gctttcggca gtgcggaaac tgccttcgcg caaatcgatc aggctctgcc cttcgacaat
     5821 tgcgccggct tcgtgcgaaa gcgcgtctgc gtcctcgcat acgggcatga ccgcctcttc
     5881 gggcatcagc cggtaggttt tgtccgccaa aatatcgccg cccgtgccgt cggcgtgcaa
     5941 atcgggggca acgcccaagg cattgagcag cttccactcg aagcggcgca aatcggcaat
     6001 atggttggct tcacggcaca ccgcctccat cacttttgcc aacgcgtcgt acagttcggg
     6061 catcggatct tcgcgcgcgg tcagtttcaa caccagttcg ttcacataca gaccgctgaa
     6121 caacgccctg ccctgcggct gccgccatcc gccgacccat tcggcacggt gcagggtttt
     6181 gagttcctga ctgccgtacc acgacgcact gacagggacg aacggaacca atacgccgcg
     6241 cagctcgctc tgccgtttgc gcgcgcttct tgccaaaagt gccacacgcc cgtaacggcg
     6301 gctgaaggct tcgacccgca ggctgctttc gcgccagggc gatgatgcca acaggaagac
     6361 gggttcgtgg ttgatgcggt gttcggacat catgcctgaa atgccgtctg aacgctgccg
     6421 ccgctacaaa acggcggtcg ggtacgaacc gatgactttg acgaacgaag cgcgttcgcc
     6481 caagcgttcc aatgccgtct gaatctgcgc gtcccggcgg tgtccttcga tgtcgatgaa
     6541 gaacaggtat tcccacaaaa cggatttgct cggacggctc tcaaacttgg tcatggaaat
     6601 acccgactcg gtcagcggtt gcagcagcga ggcaaccgca ccggcgcggt tgggcgcgga
     6661 aacggccagc gaagtcttgt cgctgctgct tgcaccggtt tcgtgatgtc ccatcaccaa
     6721 gaagcgcgtg gtgttgttcg gttcgtcttc gatgcactcg gcaaccatat cgagtccgta
     6781 gatttccgcc gccgtgcgtc cggcgatggc ggcaaccgta ccgtcgtccg attcggcaac
     6841 cagccttgcg gcttcggcat tgctggacac ggcaatccgt tcggcgttgg gcaggtgtct
     6901 gcccaaccag tcgttgcact gcgccaacgc ctgcgcgtgg gaaaagactt tggcaatgcc
     6961 ttcggtgctg ccgttgtttt tacgcaaaag gttgtggtgg atgcgcaaaa cgacttcgcc
     7021 gcacgcctgc aacgcggtaa cggcaagcaa atccaacgtg cgcccgaccg agccttcggt
     7081 cgagttttcc acgggggcga ccagatagtc tgcctgacgg gtttcaacct gcttgaagca
     7141 gttgtctatg gtcagacacg ccatggtgtg cgcggcgtgt ccgaaatgct taatcgccgc
     7201 ctgctgggta aacgtgccct gcggccccag ataggcgatg gtcagcgggc gttcgacggc
     7261 gaggcactcg ctcatcactt cccgaaacag gcgtgctacc gattcgtcgg gcagcgggcc
     7321 tttgttcaaa tcctgaatgc ggcgcaacac ggcgacctcg cgctccggac ggtatactgc
     7381 gcccgtgcct ttcagctcgc cgatggcgtg ggcgtgttgc gcgcgttcgt tgagcaggcg
     7441 caggatttcg gcatcgatgg tgtcgatggc gttgcggtgg ggaaggagga gttcgtcgat
     7501 agtttgggac ataatttcgc ctgaacagta atttatcgtg cattctaaca tcaaatgcca
     7561 tctgaaacgg tttcagacgg catttgatgt taaaggtatt cgctctgttc gttttctttc
     7621 cacgcacgat gtgagcagag ccacagaagc gcaatgccgc aagaggtgga cgcggtcatg
     7681 gttaccgcca tcacggttgc cgaaccgttg tgcaagaagg tcgccgccat acccacgccc
     7741 gcgccgatta aggactggaa cacgcccaac acggcgttcg cgctgccgcc ctcttctttg
     7801 aaataagaca taaagcatgc ctgcgtgttc gcaccgacca agccctgcgt gccgacggaa
     7861 aacatcacgc acgcaaccag cagccaaaac gggggcaacc cgaaaaacag cacggcggcg
     7921 agttgggaca ggttggcggc aaattggacg acaatccccc atagcaggat gctttgcgga
     7981 tgcgcgccgg ttttgagccg ccacgcggta atgcggttga aaaacatcat cgtgatgatg
     8041 ttgagtgcaa acgcccaagc gtattgatga ggcgtaacac ggtaaagctg ctggtacacg
     8101 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
     8161 taacccatcg cggcacgggt tttcaatacg cgtttgaacc gcccagccac cagcccgaac
     8221 acatcccgtc cgattttgcc tccgacggca ggcttgggca ggaaatactg caccaaaccg
     8281 agcagcacca gcgaatacgc cgccagaaaa acaaaaatcg cctgccagcc acccaagccc
     8341 tgcaacagtg cgccgaccat gggtgcgacc agcggcacaa ccatcaaaat gatgccgata
     8401 agggcaaaca tctgggcggc tttgcgtccg gaataataat cgcgcaccat tgcgccgacg
     8461 atgaccacag tcatgcccgc accgaatgcc tgcacgacgc gcaggttgag gagctgttcg
     8521 gcactcgaag caaatacgat ggcggcaacg gcaaggcagt agacggccag tccggtcagc
     8581 gcgacgggtt tgcgcccttt gatgtcggac accgaaccgc cgaccacctg tccgaacgcc
     8641 gtgccgaaca taaacaaact caggctctgt tcgatgcggt ggacatccgc gttcagcgac
     8701 tgcgccattt cgggaatcgc gggcaggtag gcatcgatgg aaaacggcat cagcgtaacc
     8761 agcatcgcca tcaaaaccgc catcagtttt tcgctcattt caggataatg ggcagaaggc
     8821 ataaattctc gtccgattcg caaacaaacc gcccggcggc ggaaaacaca atacgcggaa
     8881 acggaaaatt ttaacacacc ggcacaaaca ggcggcaaca aaagccccgg caaaaccgga
     8941 cgaaccggaa cgatatagtg gattaacaaa aaccagtaca gcgttgcctt gccttagctc
     9001 aaagagaacg attctctaag gtgcttaagc acccagtgaa tcggttccgt actatctgta
     9061 ctgtctgcgg cttcgtcgtc ttgtcctgat ttttgttaat ccgctataaa atcccgtcat
     9121 tcctgcgcag gcgggaatcc ggacatccaa cgctgcagca atttgtcgga aataaccgaa
     9181 actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgat gcaaagatgg tcgttatttc
     9241 ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga gatggaaaga
     9301 tttcagccta ttcggcaatc atcaaatacc gcttcccaac tttatttttg atgcgacagc
     9361 cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga cacgggcgac
     9421 aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc gagcgaaatc
     9481 aacagtcccg aaacgttggc aggaacacgg ctcatcccct tgttccacag ccaataggcg
     9541 taccagccgc accccaaacc caaatacagc agcgacaata ccatcccgac gctccagtcc
     9601 acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga tgcggcggca
     9661 atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg cgtcggacgc
     9721 atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa ccagccgact
     9781 tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa tgccgccgcg
     9841 ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc gacaaacacc
     9901 atcagcagtg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt gtatttcaac
     9961 ccgacaaact gaagcagcag ggtcagcaca tagttgacga acgacacaat cagcaacggc
    10021 ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag tgcaggcagc
    10081 gcggcaatta gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc atagacatat
    10141 ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta aaacatagtg
    10201 agtgataaag tcagatggtc atacaaaagt atagcggat
//
LOCUS       DAWFIT010000070        10212 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFIT010000070 DAWFIT010000000
VERSION     DAWFIT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.070"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(62..136)
                     /locus_tag="WGQ85_001616"
     tRNA            complement(62..136)
                     /locus_tag="WGQ85_001616"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Glu,seq:ttc)
     gene            complement(154..230)
                     /locus_tag="WGQ85_001617"
     tRNA            complement(154..230)
                     /locus_tag="WGQ85_001617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Arg,seq:acg)
     gene            complement(340..2013)
                     /gene="recN"
                     /locus_tag="WGQ85_001618"
     CDS             complement(340..2013)
                     /gene="recN"
                     /locus_tag="WGQ85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0750998.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEECLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2197..2799)
                     /locus_tag="WGQ85_001619"
     CDS             complement(2197..2799)
                     /locus_tag="WGQ85_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0750999.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2900..3841)
                     /gene="ylqF"
                     /locus_tag="WGQ85_001620"
     CDS             complement(2900..3841)
                     /gene="ylqF"
                     /locus_tag="WGQ85_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0751000.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3922..4776)
                     /gene="rapZ"
                     /locus_tag="WGQ85_001621"
     CDS             complement(3922..4776)
                     /gene="rapZ"
                     /locus_tag="WGQ85_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0751001.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4757..5719)
                     /locus_tag="WGQ85_001622"
     CDS             complement(4757..5719)
                     /locus_tag="WGQ85_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0751002.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5723..6172)
                     /gene="ptsN"
                     /locus_tag="WGQ85_001623"
     CDS             complement(5723..6172)
                     /gene="ptsN"
                     /locus_tag="WGQ85_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0751003.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6357..7247)
                     /gene="ubiA"
                     /locus_tag="WGQ85_001624"
     CDS             complement(6357..7247)
                     /gene="ubiA"
                     /locus_tag="WGQ85_001624"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0751004.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7277..7753)
                     /locus_tag="WGQ85_001625"
     CDS             complement(7277..7753)
                     /locus_tag="WGQ85_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0751005.1"
                     /translation="MEHLFGKWLPDLPTPISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7768..8415)
                     /gene="bioD"
                     /locus_tag="WGQ85_001626"
     CDS             complement(7768..8415)
                     /gene="bioD"
                     /locus_tag="WGQ85_001626"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0751006.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8412..9713)
                     /gene="bioA"
                     /locus_tag="WGQ85_001627"
     CDS             complement(8412..9713)
                     /gene="bioA"
                     /locus_tag="WGQ85_001627"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0751007.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESSLKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2373 a         2791 c         2590 g         2458 t
ORIGIN      
        1 aatgagaaat gagaaatgag aaatgagaaa tgcctccgat ataatcggag gcattttgaa
       61 ttggcacgcc cacggggaat cgaaccccgg ttaccgccgt gaaagggcga tgtcctaacc
      121 gctagacgat gggcgcatat tgtattttct tactggcgca cccggagcga ttcgaacgcc
      181 cgaccctctg gttcgtagcc agatactcta tccaactgag ctacgggtgc agcacaatca
      241 cttgttcaag tgattaagaa agcgtgataa tacggattta tctgagttct gtctattgtt
      301 ttagactttt atttttagtt attttttaac tgattgattt taaaataaac tatttttcga
      361 cgcaagttgc agcaattctg ccgcatgttg ccgcgtcgta tcggtaatga cttctccgcc
      421 caacatacgg gcaacctctt cgacccgttg gattttatcc aatatactga tttcgctgac
      481 ggtttgctct ccctcgctgt gcttgcgcac ccgccagtgg ttttcgccgc aggatgcgac
      541 ttgcggaaga tgggtaacgg caagcacctg atgttttctg cccaacgaac gtaatgcctt
      601 gccgaccatt tcagccactc cccctccaat accggtatcg acctcatcaa aaatcagggt
      661 gggaacttgg gtatattggc tggcaacaac ctgtaaggca aggctgatac gcgccaattc
      721 gccgccggag gcaactttat tcagcggacg gggcggattg cctttgttgg cggcaacttg
      781 aaattgaacc tgctctaaac cgtgtgccgt cggcgacgaa ggcaacagga cgatgtcgaa
      841 acgtgcacct ttcatggcaa ggtgttgcat atgctcggtc gtttcgccgc tcaaacgctc
      901 tgccgcctga tggcgcatgg cagaaaggat gtgggcagct tcctgatatt cggcaaaatt
      961 gtgggcaaca ttatgctcga gcgcgtccaa atcggcggca gcttgcaggc tttgcaggca
     1021 ctcttcgatt tctgccaact tggcaggcaa ctcttcaggt tcgatccggt atttccgcgc
     1081 catccccatc agttcgccca tacgctgttc ctgtgccgcc aattcattgg gattgatgtc
     1141 gctccaacct gccacatcgc gcatattggc actgatttcg cccaattcgg cttcgatgct
     1201 tgccaacata ttcaggctct cggcaaagcg cggctcgatg ttttgcagat tggccaatag
     1261 cttttgacat tgatagatat ggcgttggat gccgttgtcg ccgtcaatct tgcttccgac
     1321 ttcttcggca gcctgcaaca gctcggcaga atgggcaagg ctgtcgtggc tttggctgag
     1381 ggcttcccat tcgccttgtt taatgtccaa ctgattcagt tcgttaaact gccattccag
     1441 acgctcccgc tcgataatga cggcatcggc gtgttcctgc gcctcttgga gggctttttt
     1501 cgcattggcc caattttgat aaagctgcct gacggtttcc gcctgcgccc tgctgcccgc
     1561 aaatgcgtcc aacaattcgc gctgagcggc ttcctgatta agcgaatgat gggcgttttg
     1621 cccgtggatg tcgataagct ggctgccgac ggctttgagt tgcgccaagg tagcggcctg
     1681 attgttgata aagctgcggc ttttgccttt ggcatcgata atgcggcgga tactgagttc
     1741 ttcttctccg tcgtttaaaa gcccctgttc atacaattct gcttttaaaa caggtaaatg
     1801 ggaaatatca aacaacgccg acaactgcgc ttcttttgcg ccgctgcgga cttggctgta
     1861 atcggcttta tcgcccaaca gcagaccaat cgcatccaaa gtaatggact tgcccgcgcc
     1921 agtttctccg gtcaatacgg taaagccgct ttgaaaatcc agattcagat tttcgacaat
     1981 gacaaaatca cgcaaagaaa gtgttagaag catcgtgagg attccttagc aattctaccg
     2041 acaaaagcaa tatcaacttt agatatacga attataacta agatttttta aattaaaaat
     2101 aagtttttcc ttaattttgc ttagtgcttg tttttatctg cttatttgtt gaaatgccgt
     2161 ctgaagcaat gtgcgtttca gacggcattt ggaatttcag ttgggcaggg tatcggacgg
     2221 tacggcttcc ggctcttctt catccgtagg cggcatttcc attaaatcgg gtaaaaccag
     2281 ttcgcccagt tcggtcagcg gcggaagttc ttccagaccg tccaagcgca aatcgctgag
     2341 aaaagttgcc gttgtcgccc acaatgcggg ttttcccaat gtgtcccgat gtccgatgac
     2401 ttcaatccac ccccgatcct gcaaggtctg catcacgttc tgcgacaccg ccacgccgcg
     2461 tatgccctcg atgtcgccgc gcgttacggg ctgctggtag gcgataatcg ccagtgtttc
     2521 catcacggcg cgggagtaac gcggcgcacg ctgttcttgc aggctgccca gccgctcgaa
     2581 tgccgtctga acaatctgaa aacgccagcc ctcttgcgta tgcaccagtt gcaacgccct
     2641 atcctgccaa cgcgttttca actgcgccaa cacatcaatc agtttgtcct gcgacagtgg
     2701 cggcacacac aattcgcgca tagatttttc ggtcagcggt tcggtttggg tcagcagtgc
     2761 ggcttcaatt agtgcgtcgg gagaaatttt gtcggtcata cgggtatccg tgctgaaacg
     2821 gcatgggttt ggatatgccg tctgaaatcg gttggagtag agagaagctg cctgaaaaat
     2881 atttttcaga cggcattctt tatgcttccg aaggcttctg ccctttacgc tctgcttttc
     2941 tggcttcgcg tatggctttg agttcggctt ctttctgacg ggcttttttg agccaagttt
     3001 cccattggtt cggcgtttcg agggtgattc tgccgatttt gccttcacgg aagtcggtga
     3061 ggatgttttc ggcggctttt tggtagttga tccgtccgcc gctgaggact gcgccgcgtt
     3121 ttttggctat ccattcgagc caaacgtttt cgtcccagtg gctgctgggg tctttgtcgg
     3181 cttggtagcg ttcttgcaac atagggaggt agtggcggcg gaggtagtct aaaagttcga
     3241 gggcgacttc ttcttcgtcc aacgcgttgc gtccgactgc gccgccggcg gcaaggttgt
     3301 agccgccttc ttcgacgatg attttcggcc acagcattcc gggggtgtcg tagagccaga
     3361 agtcatcggc gaggaagagg cgttgttcgg ctttggtgat gccgggttcg ttgccggttt
     3421 tggcggattt tttgcctatc atgccgttga tgagggtgga cttgccaacg ttggggatgc
     3481 cgcagatgag gacgcgcagg ggtttatcta tgccttggcg gtggggaatc atggcacgac
     3541 aggcttgggt aattttgccg tgtgcgcctg tttcggagga atcgagggcg atggcgcagg
     3601 tgtcggggcg gctgttatag tgttcgagcc agattttggt gcgctcgggg tcggcaagat
     3661 cttgtttgtt gaggatttta agtttaggtt tgcctttgga aagttgggca agcagggggt
     3721 tttcgctgga ggcgggcatt cgcgcatcca gcatttcaat caccatgtct acgcttttgg
     3781 cgcgctcggc gatggctttt ttcgctttgt tcatatggcc gggaaaccat tggattgcca
     3841 tgtctttcct ttcttctcaa tatttgaaat gccgtctgaa acagaaaatc gggtttcaga
     3901 cggcatcatc gttcaacggg attagcggct cggcaggttt tgcgcctgtc tgtgccgtat
     3961 cagcaattca taacgccctt tcaaccttcg ggcgagtttt tcgacaacat agacggaacg
     4021 gtgctgcccg ccggtacagc cgatggcgac ggtaacgtag ctcctgcttt catcctccaa
     4081 acgcggcagc caatgcgtca gaaatttttc gatgtcgtca accatttcct gcacaagcgg
     4141 ctgtccgtcc aaataatccc aaacgggctt gtccataccg gtgtaaggcc tcaactcggg
     4201 atcgtaatac gggttgggca gactgcgcat atcgaacata aaatccgcgt tgttcggcac
     4261 gccgtatttg aacccgaagg actccaaaat caccagcagc cctgtacgtt cgaccttcag
     4321 ccactgccgg actgcatggc ggagctgttg ggcattcatc ttggaagtgt cgatacaata
     4381 ggcgatttct ttaagcggga acagccattc ccgttctttc tttaagcttt ccaacaaggt
     4441 catatcctga ttgctcagag gatgtcctcg cctggtttcg gaaaaccggc ggaccaacac
     4501 gctttcttcc gcctcgacaa acaaaacttc aaccctgtgc cccagtccgc gcagagaggc
     4561 aatctgttcc cgcgcctgtc cgatgtcgat gccggaacgc acatcgacgc tgaccgccaa
     4621 ttcagtttcg tccgcacgct cgatatgata cgacaccagc gcgggcaaca tttccaaagg
     4681 caaattgtcc acgcagaaat aacccgaatc ttccatttgg cgcagtgcga cggatttgcc
     4741 cgaaccggac aacccgctaa tcaggacgat tttcatgatt ctgttcgttt tctttaagtt
     4801 gcgtctgatg gcgttccaaa aattcgcgcg tactgtcctt accgcgcaac tgcaaaatgt
     4861 aattgcgtac cgccgcctca accaaaacgg cgaggttgcg tccgacggcg acgggcagcg
     4921 taaccgaacg gacgttgacg ttgaggatgg attcggtttc ggtgcggata ctcaaccggt
     4981 cgagctgctt catatactcg tcgtctgcct cgaccaaatt gataatgagt tgcaggattt
     5041 ttttggggcg gatggaagtt tcgccgaaaa tatggcggat attgagtatc cccaagccgc
     5101 gcacttccaa aaaatcacgc agcataggcg aacaacgccc ttccaatgtt tccggaccga
     5161 tgcggaacag ctcgaccgca tcgtcggcaa tcaggctgtg gccgcgcgaa atcagttcca
     5221 acgccaattc gctcttgccc aagccggaat gtccggtaat cagcacgccg atttcaaaca
     5281 catcgagaaa tacgccgtgt ttgacggacg atgccgccag ggtgcgttgc aggtaaatcc
     5341 gcaacacgtc catcagatag gggctttcga gtttggaagt cagcagtgga atatcgtttt
     5401 tatggcaata gtcgcgcagc cccggggaaa ccggcaagcc gtttgccaca ataaccaaag
     5461 acatagaaat gtcgaacagg tcgccgaact gataacccgt ttcccccgat tcgagacggt
     5521 tcagatattc cgattccgcc aaaccgacca cttggatttg gttggggtgg atgaaattca
     5581 ggtgtccgac caaagcgagg acgggcttgt ccgcctctac gccgatacgg ttgtccgcac
     5641 ccgaattgcc ggcggcccag gcaagttgta acttgtattg gttgtcgtca aacagtcgac
     5701 ggacagagat actgggcata ttttattctt cggtcaggat ggcgcgggct tcttccgcag
     5761 aagaaaccgt catcagcgat tcgcggatgc ttttttggga aaacttgccg gccagtttgg
     5821 acaatacctc caaatgctcg cccgttgcat tctcaggaac cagcaagata aaaatcaggg
     5881 aaaccggctt gccgtccggt gcgtcaaatc cgacgggttc gcgcgtgcgg atgaacgcgc
     5941 ccgtcgcctg cttcacgccg gcatgacggc cgtgcgggat ggcaacgccc tgccccaaac
     6001 cggtcgaacc gagtttttca cgggcaaaaa gacattcgaa aacatcagca tgggacaatg
     6061 aggattcgcg ttccaaaagc aggcccgctt cctcaaacag ccttttttta ctgcccacct
     6121 ccatatccaa aacaatatgg gacaaaggca aaatttcgcc gataaggctc ataagcttct
     6181 cttttcagac atcgcaaaac agaaagattg taccgactgc tagggcaaat ctcaatcccg
     6241 catacggtac gggctgacat aacacagcgt tttaaaaaca tattttaacg cttttcggca
     6301 cagatagaaa tgccgtccaa agcagtttac ggctcttcag acggcattgc cctgccttat
     6361 ttcgcgaaaa acgtatgggc aaaaatggcg gtaaaccaca cccaaccaat tctgttgttt
     6421 gccaaaaacg tttcaaaaca gatttgccgg acgcggcttt tgatggcggc atattggcgg
     6481 tattgcagca gcaggacgat ggggattgcc gtccaatatg cccaagccgc gccgataacc
     6541 gcacccaata ctgccatcag cagggtaaag ccgccatgac acagcataac agcggcgatg
     6601 tcgtaacgcc cgaatgtgac ggcggaggtt ttgatgccga ttttcaaatc gtcttctttg
     6661 tccgccatgg cgtaaacagt gtcgtaagcc agtgtccata aaacattggc ggtaaaaagt
     6721 atccatgctt caacggggac gctgttgccc actgcggcaa acgccatcgg gataccgaag
     6781 gaaaaggcaa gcccgagata gagttgggga atcggaaaaa aacgtttggt aaacgggtaa
     6841 gtcagcgcaa gaaacagcgc gggcaggctc atcagccaag tcagatgatt cagcggaatc
     6901 aggcacaatg cggcaagcag gcacaaaaat gccgtcagca gcagcgcttc ttttttcttg
     6961 accctgccct gtgcgaacgg acggttttta gtgcgctcga cagccccgtc aaaatcgcgg
     7021 tcggcaaaat cgttgatgac gcagccggca ctgcgcatta aaaacgtacc gattgtaaac
     7081 gccgccaata ccgccaaatc gggaatgccg tctgaagcca gccacaatgc ccagtaggtc
     7141 ggccacagta aaagcagcgt cccaatgggc ttgtccgccc gcatcaggcg caggtacaca
     7201 tccaaacggt cggacagtcg taaaaataaa ggggatttag gattcatatt gccgcacagc
     7261 ttgaaaaaac ggtattttat ccgataaagc gtttcagttc gggcagaaaa tactcggtca
     7321 gcagcatttc ctcgccgtga cgggaaaacc gagaacgccg cgcggcaaag taccgtccgc
     7381 atccttcgcc ggaaacggca aactcaaacg ccgaacgcgc cccttccaaa tcggcttgaa
     7441 acagacgctc gcccaaaggg cgcgtgccgc agtccaaaat gttttgccaa aacgccgaac
     7501 cgatacggca ctcgctcctt gccgcgacaa cagggatacc gtccagcttc aacaaaactt
     7561 cgcgcaccag cctccctccg cattccgtct ccaattcgcc cagtttcagc agttccaccg
     7621 aaaatgtatg cggcaaggcg cgcaatgcgg cggtcagcga ccgggtgtgc agcagccgca
     7681 ccatcggcag gctgatgccg tctgaaatgg gggtgggcaa gtcgggcagc catttcccaa
     7741 ataggtgttc catatttttc ccaatcttta taccgcgtct gtttttgcca actccatcca
     7801 ttccgcttcg gaaaaatcgg ctttcagacg gcatttcaag tagttcaggc tgtcttgggc
     7861 gatgtgtgcg tcgcggctgt cgtggatgtg gttgaacacg aggctgtgca gacggatgcc
     7921 gtattgtttg agcgcggcga aactgagtaa agtgtggttg atactgccca gccgtccgct
     7981 ggtaacgagg atgacgggat agccttgctg acggatataa tcaatggtta acaggttttc
     8041 cgtcagcgga accatcaatc cgcccgcgcc ttcgaccaag acgatttcgt attgcgccgc
     8101 cagttgctgc gtggcggtgc ggattttgtc caagtccaaa gccctgccgt caagacgggc
     8161 agcgagatga ggcgaagctg gataactgaa gatttcaggc atggtcagcc cttgtttgtc
     8221 ggcttcctgc atgggtttac ccatgatttt acgatggacg gcgatgtcgt cggcaatgtt
     8281 ttggcaaccg gtttgcacgg gcttttgcgt aatcacgctt ttgccctgct gcaacaattg
     8341 ttttgccaac acgccggtgg cgacggtttt gccgatgtcc gtgtctatgc cgctgacgaa
     8401 gtaaacgcct ttcatttgct gtgttccttc aagatttgca cggttttgtc ggcaagtttg
     8461 gtcaaaacgc cgtctgaaat gatgtaaggc ggcatcagat acaccagcct gccgaacggg
     8521 cgcacccaaa tgccctgcgc cacgcaatcc gcttgaaaac gcgccatatc cacgcctttt
     8581 tccagctcga tcaccccgat ggcacctaaa acgcgcacgt ctttcacgcc gcgaatgtcc
     8641 cacgcggctt tcagacggcc ttttaagctg ctttcaatgc ggcggatatt tgcctgccag
     8701 tcttgagaca aaagcagttt gaccgaagcg caggcaacgg cacacgccag cgggtttgcc
     8761 ataaacgtcg ggccgtgcat aaacacgccc gcttcgccgc gcgaaatcgt ttcggtaact
     8821 ttttgcgaag tgattgctgc cgccagcgtc atatagccgc cgctcaaacc cttgccgatg
     8881 cacataatat ccggcacgac ctccgcgtgt tcgcaggcaa acatcttgcc cgtgcgcccg
     8941 aatccggtgg cgatttcatc aaaaatcaaa acgatatcaa actcgtcgca caaatcgcgc
     9001 aatccgcgaa gatactgcgg atgataaaaa tacatgccgc ccgcgccctg cacgaccggc
     9061 tctaaaataa aggcggcaat atccgcatga tgcacttcaa acaaggcgcg gacaggctgc
     9121 aaatccgccc cgtcccattc atcgtcgaaa cggcttttcg gattatcgac aaaataacgc
     9181 tgcggcaacg cgctgccgaa aatatggtgc atccccgttt ccggatcgca gacggacatc
     9241 gcgttccaag tatcgccgtg atacccgcgc cgtaccgtcg caatattctg cttcgccgtc
     9301 aacccccgcg cctgctggta ttgcaccgcc atcttcaacg cgacttccac cgaaaccgaa
     9361 cccgaatccg cataaaaaat acgatccagc ccctgcggca aaatcccgac caacaatttg
     9421 cccagctcca ccgccggctc atgcgtcaaa ccgccgaaca tcacatgcga catctgtttc
     9481 atctgcgtct caaccgcctg attcaaaacg ggatgattgt aaccgtgtat cgcacaccac
     9541 caagacgaca tcccgtcaat cagccgcgtg ccgtccgcca attcgataaa caccccttct
     9601 gcacgtttga caggataaac gggaagcgga tcggtcatgg aagtataggg atggagcaga
     9661 tgggtacggt cgaaatcaag caatgatgat gtgtgttgat gttcagacgg cataagtttc
     9721 tctcttttct tattgctgca ttcaaacgca aaacgcgtat tctactccga cagaccgctt
     9781 cccacacctt tccatccgtt tcgggcgtaa aaccacgcaa caaatcgtcc gcagtataag
     9841 cgcacaccgt ttcgcattcc ccaagcccga ttggaatcag acgacccaac gcccaatatt
     9901 gtttcacaca gcctccgccc aacgcttcaa tcagtcatag acaaaacggc aatccatacg
     9961 atacagatca tagcaacagc catcgcaaca gcgttagcaa aatcagggga ctccgacata
    10021 ggcgcatagc atctaccaat gcacagctcc tcattcggct ctatgaatac catacccatc
    10081 acaaaatcca ccgccaaaac caggcacggc ttcttatact tatgatagat ttccaccatc
    10141 ctgccccata tatcccaaac attcataccg catatcccgc aggcaacaaa ttccgattga
    10201 aggttacagc cc
//
LOCUS       DAWFIT010000071        10130 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFIT010000071 DAWFIT010000000
VERSION     DAWFIT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.071"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..382)
                     /locus_tag="WGQ85_001628"
     CDS             complement(<1..382)
                     /locus_tag="WGQ85_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751008.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKFNGIPKAHF
                     ELYLKGYERRFNNSEIKVQISILKQ"
     gene            complement(688..1947)
                     /gene="rho"
                     /locus_tag="WGQ85_001629"
     CDS             complement(688..1947)
                     /gene="rho"
                     /locus_tag="WGQ85_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0751009.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2167..4551)
                     /gene="ppsA"
                     /locus_tag="WGQ85_001630"
     CDS             complement(2167..4551)
                     /gene="ppsA"
                     /locus_tag="WGQ85_001630"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0751010.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4962..5783
                     /locus_tag="WGQ85_001631"
     CDS             4962..5783
                     /locus_tag="WGQ85_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0751011.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(6023..6685)
                     /locus_tag="WGQ85_001632"
     CDS             complement(6023..6685)
                     /locus_tag="WGQ85_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0751012.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6698..7525)
                     /locus_tag="WGQ85_001633"
     CDS             complement(6698..7525)
                     /locus_tag="WGQ85_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0751013.1"
                     /translation="MKPDIYALLELALLSDDPDEKGQLTDEAFAAVQNIDGAEANAPP
                     LDFRHAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            7802..8425
                     /gene="lolA"
                     /locus_tag="WGQ85_001634"
     CDS             7802..8425
                     /gene="lolA"
                     /locus_tag="WGQ85_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0751014.1"
                     /translation="MMKPHKLFQFLAVCSLTAAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKT
                     AQDRAIGGSPAAILSDKAALEAGYALEEDGSSGGIDYVLATPKKNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGSLNTKPGLSRGAFKFTPPKGVDVLSN"
     gene            8698..9837
                     /locus_tag="WGQ85_001635"
     CDS             8698..9837
                     /locus_tag="WGQ85_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0751015.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9969..>10130
                     /locus_tag="WGQ85_001636"
     CDS             9969..>10130
                     /locus_tag="WGQ85_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0751016.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT         2278 a         2564 c         2836 g         2452 t
ORIGIN      
        1 attgttttaa aatggaaatt tgaactttta tctcactgtt gttaaaacgc cgttcgtacc
       61 cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt aaatgacgtt
      121 ttgcccgatt ccaaaagttc tcaattccat taatatggtt ttgtcgttca gcaaaataac
      181 tttcatctgc ttctacttcg ccatcaaaca tttccaaatg tggactgttt tgataaataa
      241 gtaatcgtaa acgatgaaaa taataggctg cggtactttt attaacgcct actaactctg
      301 ctgctgttct tgcagttaca cctgcgacaa acagttcaat gagtttattt tgtttatacc
      361 ggcttagacg actttttctc ataggggcaa ctctaactta atttgaattt ccctagttat
      421 ctaggacagc cccaaattta tacaaaaatg agcgcggttc ggcgcaacct tgaatcaagt
      481 tcccgcatcg gttttcattg ccggtacgga tgcgttcaag ccggttttgc aaaggccgcg
      541 ctttcggcaa gcggacacgg acactgccga cggttgcgcc gttaacgggg ggagggagga
      601 gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc gattttcagg cttcagacgg
      661 catttcgcat taatgcgggc ggcgcgttta tttgccgcgc atcagttcaa agaaatcgtc
      721 gttgttttta gaggctttga ttttcccgtt taagaactcg gtagcttcga tttcatccat
      781 cgggtgcagg aacttgcgta agagccacat acgttgcaac tggtcgttcg gcacaagcag
      841 ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg gggaagaggc gtttttccgc
      901 catacggcgg tcgaggtgca attccatatt gccggtgcct ttgaattctt cgtaaatcac
      961 atcgtccata cggctgccgg tttcaaccaa tgcggtggca atgatggtca gcgaaccgcc
     1021 ttcttccacg ttgcgcgctg cgccgaagaa acgtttgggg cggtgcagcg cgttggcatc
     1081 gacaccgccg gtcaggattt tgcccgaggt ggggacgacg gtattgtagg cgcgggcaag
     1141 gcgggtaatc gaatccagca ggatgaccac gtcttttttg tgttctacca tacgcttggc
     1201 tttttcaagc accatttcgg caacttggac gtggcgttgc gccggttcgt caaaggtgga
     1261 ggaaaccact tcgccgcgga cggaacggct catttcggtt acttcttcgg gacgctcgtc
     1321 aatcaagagg acgatgagct cgacttcagg atagtttgcg gtaacggcgt gggcaatatt
     1381 ttgcaacatc acggttttac cgcttttggg cggggcaacc aagagggcgc gctgaccttt
     1441 gccgatgggg gaaatcaggt cgatagcacg tccggtcagg ttttcttcgg actttaagtc
     1501 gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc aggttttcaa acaggatttt
     1561 atggcggcat acttccgggt ggtcgccgtt gatggtatca agcctgacca gggcaaaata
     1621 gcgttcgttg tcttttggga cgcgcacgct tccttcgatg gtgtcgcccg tatgcaggtt
     1681 gaagcggcgg atttgggtgg gtgagacata gatgtcgtcg ggaccggcaa gataggacgt
     1741 gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt tcaagcgtgc cggagcaggt
     1801 gaaaccctcg ccttttttca tcatctggcg gacgatggca aatacgaggt cttgtttgcg
     1861 gaatcggttg gcgttttcga tgccgtgttc ttccgccaat tctaagagtt tggaaatgtg
     1921 cagggtttgt aattcggaga cgtgcataat aatgatgtat tttgaagagg aaaaagacag
     1981 gcagatgccg tctgaaagaa gaagctgacc gttgccggtt gctcggggaa gggggaattg
     2041 taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg gggcatcggc agaaaatgcc
     2101 gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt ttcaggctcg ggggtatggg
     2161 cattgattat ttgttcaatt cattcgccaa atacagccaa gtttcgatga cggtatccgg
     2221 gttcagggaa acgctttcga taccttcctc aaccagccat ttggcgaagt ccggatggtc
     2281 ggacgggcct tgaccgcaga tgccgacata tttgttctgc ttgcggcagg cggagatggc
     2341 aaggtgcagc atcactttga cggcagggtt gcgttcgtca aacgattcgg ataccaagcc
     2401 gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg ttcgagccga tggagaagcc
     2461 gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg ctcggcagct cgcacatcat
     2521 aatcaggcgc aggccgtttt tgccgcgttc caagccgttt tctttcaggg ctttgacaac
     2581 ggcttcggct tcgcccaaag tgcggacgaa cggaatcatg atttcaacgt tggtcagacc
     2641 catttcatcg cggacgcgtt tcaaggcttt gcattccaag gcgaaacagt ctttgaagtt
     2701 gtcggcgaca taacgcgccg cgccccggaa gcccaacatc gggttttctt catgcggttc
     2761 gtatacgttg ccgccgacca ggttggcgta ttcgttggat ttgaagtcgg acatacggac
     2821 gatggtttta cgcggataaa ccgatgcggc caatgtcgcc acgccttcgg cgattttatc
     2881 gacgtagaag tcgacagggg acgcgtaacc ggcgatacgg cgggtaattt ccgcttttaa
     2941 ttcgtcgtct tgtttgtcaa attccaacaa ggctttcggg tggataccga tttggcggtt
     3001 gatgataaat tccatacgcg ccaagccgat gccttcgctg ggcaggttgg cgaagctgaa
     3061 tgcgagttcg gggttgccga cgttcatcat gactttgacg ggtgctttgg gcatattgtc
     3121 caaggcgaca tcggtgatct gtacgtccaa caggccggca tagatgaagc cggtatcgcc
     3181 ttcggcgcag gatacggtca cttcctgacc gttttccaag agttcggtcg cattgccgca
     3241 gccgacgacg gcaggaatac ccagttcgcg cgcgatgatg gcggcgtggc aggtacggcc
     3301 gccgcggttg gtcacgatgg cggaagcacg tttcatcacc ggttcccaat ccgggtcggt
     3361 catatcggta acgagtacgt cgccggcttc gacggaatcc atctcggaag catctttaat
     3421 caggcgtacc ttgccctgac cgactttctg accgatggca cggccttcgc ataatacggt
     3481 tttatcgccg ttgatggcga agcggcgcag gttgcggctg ccttcttcct gggatttgac
     3541 ggtttcgggg cgggcttgca ggatgtagag tttgccgtcc aagccgtcgc gtccccactc
     3601 gatgtccatc gggcggccgt agtgtttttc gatggtcagt gcgtaatgcg ccaactcggt
     3661 gatttcttcg tcggtaatgg agaagcggtt gcggtcttct tcggggacat cgacgttggt
     3721 taccgatttg ccggcttcgg ctttgtcggt aaaaatcatt ttgatgtgtt tcgaacccat
     3781 ggttttacgc aggatggcgg gcttgcccgc tttgagcgtg ggtttgaaca cataaaattc
     3841 gtccgggttg accgcacctt gtacgacgtt ttcgcccaga ccgtaagagg aggtaacaaa
     3901 gacgacttga tcgtagccgg attcggtgtc gagggtgaac atcacacctg atgcgccgct
     3961 gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg acgatatcgt gttcgaagcc
     4021 tttgtggaca cggtaggaaa tggcgcggtc gttgtacagg gaggcgaata catggtgcat
     4081 cgcttcttta acgttttcca atccgttgat gttcaagaag gtttcctgtt ggccggcaaa
     4141 tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt acggcaacgg aaatgtccgc
     4201 gccgccggca tcggcaacca ttttgttcca tgccgcttcg atttcggcat cgagctgttc
     4261 ggggaagggt gtgtccaaaa tccattggcg gatttctttg ccgacgcgtg ccagttcggc
     4321 aacgtcttcg acatccaatt ttgccagtgc ggcggaaatg cgttcgctca gaccgttgtg
     4381 tgcgaggaat gcgcggtagg cttcggctgt ggtggcaaag ccgccgggga cgcgaacgcc
     4441 tttttcggtc agctgactga tcatttcgcc cagcgaggcg tttttaccgc ccacgcgttc
     4501 aacatctgtc atacgcaggt tttcaaacca aattacgtag ttgtcggcca tttgtgtgtc
     4561 caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct tattttaagc gattcgttcc
     4621 tctgctgtca tgtgttttat ccgttttaaa atcatgatgc cgtctgaaaa attgcggttt
     4681 cggcgtgtgt agcggtttga aacttgcagc cggtatactt ctttttttgg gtattttctt
     4741 tgtaaaacag gtggtttgaa taggttaatg ttttttctgt ttgatttttt tgtttatttt
     4801 ttaaaatttt ctgccaaaaa atactttata taaataattt tatttcaaaa ttatattgtg
     4861 tctgtttggg tgtaatccga ggtaggcgtg ctgcgggtgc tttccttgtg tctgccgctg
     4921 ctgttatgat gatattttta ttctgtattt aaggatggaa gatgagcagt ccgcgccatg
     4981 tgttttacat ttccgaccgt accggtctga ctgccgagaa tatcggcgag gcgttgctga
     5041 accagtttgg caatctgtcg ttcaaacgcc atacgcatcc gtttgtcgat acgccggaaa
     5101 aggcgcgcgc ggtggtggag aaggtcaatc ggagccggca ggaaaacggt cagcgtccga
     5161 ttgcgtttgt cagtgtggtt gatgacgaaa tccgtcggat tatcaaaggg gcggatgctt
     5221 ttcagattaa tttctttgag acttttttgg gactgttgga gaaggaactc aataccgaag
     5281 ccacggcatc cgagcagggg catcacagta tcggtaatac gaagcgttac gatgcgcgta
     5341 tggaggcggt aaatttctca ttgaaccacg atgacggggt cagcgataag aaccttcagg
     5401 aagcggatgt aatcttgatg ggtgtatcgc gttcgggcaa aacgccgacc tgcctttacc
     5461 tcgccctgca atacggcatc cgtgcggcaa actatccgct gattcccgac gatttggaat
     5521 cggccgatct gccgcgtatg gtcaagcctt atagggataa gctgttcggg ttgaccatcc
     5581 agccggaacg tttgcaggcc atccgccaag agcgccgccc gaattcaact tatgccaaaa
     5641 tcgatacatg ccgcagcgag gtggcagacg cgcagagtat gttcagacgg catgggattc
     5701 cgtttgcgaa tacgacggat aaatcagtgg aagagcttgc cgtacacatc cttcaggcgt
     5761 gcaagctcaa acgcaggttt tgacgggttt tgattcggtt tgaaggtgga aatgccgtct
     5821 gaaatcaggt ttcagacggc agttttatag tggattaaca aaaatcagga caaggcgacg
     5881 aagccgcaga cagtacaaat agtacggagc cgattcactt ggtgcttcag caccttagag
     5941 aatcgttctc tttgagctaa ggcgaggcaa cgctgtactg gtttagattt aatccactat
     6001 atgtttgtgg ggcggatatt tttcagggct gtattttgtc cagacattcg agcagatcga
     6061 gtggcgtgcg gatgtggaaa tccgcctgcc atgagccggt atcgtcttcg ggagcgatgt
     6121 agccccattc ggcgaggacg gttttcatgc cggcgttgcg gcctgcttgg atgtcgcgtt
     6181 cggcatcacc gacgtagagt gtgtgttgcg ggtcggcgtg gatttgtccg cacgcataca
     6241 gcatgggttt gatgctgggc ttgggttcgc cgcaggtgtc gccgctgacg acgacggcgg
     6301 gtgggatgat gaagccgagt ttggggacga gtttgtcggt gaagcgcatg ggtttgttgg
     6361 tgatgatgcc ccatttgatg ccgcgttggt tgagttcggc aatgagttcg ttcacgccgt
     6421 caaagagctc ggtgtcttgg gcgtagcggc ggtcgtattc ctccagatat tcggtgcgcc
     6481 atcgggcata gtcggggtgg tcgggggtga tgcctgcgcc gagtttgatc agtcctgccg
     6541 cgccgtggct ggcttgggtg cggatctcgt ccatgctttt tgcaggtagt ccgtggcgag
     6601 cgagcagggt gttgagtgcg cctccgaggt cgagggcggt gtcggcaagt gtgccgtcga
     6661 ggtcgaagag tacggcgtgt atcatgtgtg ttccttttca gacggtattt ttgttctgtt
     6721 tcaaaccctg ctcgaaatct tccagcatat ccaattcaaa gcggctgaag cccgcttttt
     6781 cgcgcgcttc gatgttcaca tagccgcgga agataaacat atcgtaacgg gtaatcagac
     6841 tgcggaacag ggcgacaggt tccaaaccgc gttcgcggca gaggtgttga taccaatggt
     6901 tgccgatggc gacatggccc acttcgtcgc ggtaaatgat gtccaatacg ccgcaggttt
     6961 ccgaatcgcc gcgctgcgcc accttcgcgc gtatccccgg cgtaacgtcc agcccgcgcg
     7021 cttccaacac gcgcggtacc aacgccatac gcaacagcgg gtcgtaggcg gttttgtacg
     7081 ccatatccca caaatggttg tgcgcctcga aactgccgta atcgaagccg aaagcgcgca
     7141 atctgtcgcg catcaggcgg aaatggtaca cctcttcctt cgccactttc acccagtcgc
     7201 ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc gtccaaagcc agattgacgg
     7261 cgttgaattc gatatgcgca atcgcgtgca gcatcgccgc atagccttct gccgtgttca
     7321 ttttgcgtgg cgtcagttgc gacggcgcga ccaaaacagg cttgtccggc cgtcctgcgt
     7381 ggcggaagtc cagcggcggc gcgtttgcct ccgccccgtc aatgttttga acggcggcaa
     7441 acgcctcatc cgtcagctgt cctttttcat cggggtcgtc tgaaagcagg gcaagttcca
     7501 gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg tttggaaaac aaatattata
     7561 gcgtttaaaa aaaacaagat gaggcatata atctccgcaa ttcggcattc cgcgcccaaa
     7621 ccgtcaaata acccggcaca catttaaagg gcggcgtggc acgcctgcct tttctcgggg
     7681 gcaaaaaaat cagccgtcgg aaaacgcggt ttgcaaaatg ccaaccgccc gtcccaccgc
     7741 ccgtatgatt gttttgccac gccgatactt tacgccacac ccgttccaac aaggaaaaat
     7801 aatgatgaaa ccgcacaaac tgttccaatt cctcgccgtc tgctccctga ccgccgccgt
     7861 cgcttccgca caggcgggcg cggtggacgc gctcaagcaa ttcaacaacg atgcagacgg
     7921 tatcagcgga agcttcaccc aaaccgtcca aagcaaaaag aaaacccaaa ccgcgcacgg
     7981 cacgttcaaa atcctgcgtc cgggcctttt caaatgggaa tacaccaaac cttacaggca
     8041 aaccatcgtc ggcgacggtc aaaccgtttg gctctatgat gttgatttgg cgcaagtgac
     8101 caagaccgcc caagaccgtg ccatcggcgg cagccccgcc gccatccttt ccgacaaagc
     8161 cgccctcgag gcaggttacg cccttgaaga agacggctcg tccggcggca tcgactatgt
     8221 tttggcaacg cccaagaaaa acaacgccgg ctaccaatac atccgcatcg gcttcaaagg
     8281 cggcaacctc gccgccatgc agctcaaaga cagcttcggc aaccaaacct ccatcagctt
     8341 cggcagcctg aacaccaagc ccggcctctc gcgcggcgcg ttcaagttta ccccgcccaa
     8401 aggcgtggac gtgttgagca actgatgccg tccgccccga tgccgtctga acgccgccga
     8461 ggcttcagac ggcattttta cgcaggcgga acaatgtccc gcattaccgc ccgatcgggc
     8521 atcggaacgg caaacccgtg aaaattaacg gttgcgcccc ggccgttttt gccgtttaat
     8581 gcaaaccttg ctgcactaag ggccaagaaa gccgaccggc cgcccccaca gcttccgatg
     8641 caggcggccc ggccgtccct gcaatgtttt ttatttttga acgaaaggtc gaaaaccatg
     8701 aaaaaaacac tggtggcggc ggcaatcctg agcctcgcct tgactgcgtg cggcggcgga
     8761 agcgataccg ccgcccaagc cccctccgcc aagcccgaag ccgaacaatc gggcaaactc
     8821 aatatctaca actggtcgga ttatgtcgat cccgaaaccg tcgccgcctt tgaaaaagaa
     8881 accggcatca agacgcgttc cgattattac gacagcaacg aaacactgga ggcaaaagtc
     8941 ctgaccggca aatccggcta cgacctgacc gcgccgtcca tcgccaacgt cggccggcaa
     9001 atcaaagcgg gcgcgtatca gaaaatcgac aaggcgcaaa ttccccatta cggcaacatc
     9061 gataaagatt tgttgaaaat gatggaagcc gtcgatccgg gcaacgaata cgccgtcccc
     9121 tatttctggg gcatcaacac cttggcaatc aacacgcggc aggtgcaaaa ggcattgggt
     9181 acggacaagc tgcccgaaaa cgaatgggat ttggtgttca aacccgaata caccgccaaa
     9241 ctcaaatcct gcggcatcag ctatttcgac agcgcaatcg aacagattcc cttggcgttg
     9301 cactatttgg gcaaagaccc caacagtgaa aatcccgaag acatcaaagc cgccgtcgat
     9361 atgatgaaag ccgtccgggg cgacgtgaaa cgcttcagct cttccggcta tatcgacgat
     9421 atggcggcgg gcaacctgtg tgccgccatc ggttacggcg gcgatttgaa cattgccaaa
     9481 acccgtgccg aagaagccgc aaacggcgtg gaaatcaaag tattgacccc gaaaaccggc
     9541 gtgggcgtgt gggtggattc ctttatgatt ccgcgcgacg cacaaaacgt tgccaacgcc
     9601 caccgctata tcgactacac gctccggccc gaggtggcgg cgaaaaacgg cagcttcgtt
     9661 acctacgcac ccgccagccg ccccgcgcgc gagctgatgg atgaaaaata cacctccgat
     9721 gcatcgattt tcccgaacaa agaactgatg gaaaaaagtt tcatcgtatc gcccaaatcc
     9781 gcagaatccg ccaaactggg cgtgaagctg tggcaagggc tcaaagcggg caaataaccg
     9841 gactccctgc cgtctgaaac ctttcgggcg gcaggaaacg gcgcgtccgt tatcaaacag
     9901 ggggcgtttc ccctcctgcc ggttatgatt gggttaagat taaaatgatt tagtaaaatg
     9961 agaaagatat ggatttaagt atcgtagttc ccatttataa tgtcgaaagt tatttggaag
    10021 cgtgtttaag ttccatagaa cctatattaa gtaatgaaaa tgtcgaactt atccttgtga
    10081 atgacgggtc aaaagacgga agtgaagata tatgttataa atatatagat
//
LOCUS       DAWFIT010000072        10090 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFIT010000072 DAWFIT010000000
VERSION     DAWFIT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.072"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(135..1352)
                     /locus_tag="WGQ85_001637"
     CDS             complement(135..1352)
                     /locus_tag="WGQ85_001637"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0751017.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAIADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1553..2281)
                     /gene="rph"
                     /locus_tag="WGQ85_001638"
     CDS             complement(1553..2281)
                     /gene="rph"
                     /locus_tag="WGQ85_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0751018.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNSKVICTA
                     SVDENVPSFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALHIAVGKLISDGILTENPIRE
                     AVAAVSAGIVGGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLEELG
                     KLIGLAEKGIRELVRYQQNALSAA"
     gene            complement(2363..2827)
                     /locus_tag="WGQ85_001639"
     CDS             complement(2363..2827)
                     /locus_tag="WGQ85_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0751019.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3044..3820
                     /locus_tag="WGQ85_001640"
     CDS             3044..3820
                     /locus_tag="WGQ85_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0751020.1"
                     /translation="MSNNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIRHLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVECKQPFVLSGGAGGQKDP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4065..4919
                     /locus_tag="WGQ85_001641"
     CDS             4065..4919
                     /locus_tag="WGQ85_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0751021.1"
                     /translation="MLPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4960..6168
                     /gene="pncB"
                     /locus_tag="WGQ85_001642"
     CDS             4960..6168
                     /gene="pncB"
                     /locus_tag="WGQ85_001642"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0751022.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6260..7978
                     /gene="argS"
                     /locus_tag="WGQ85_001643"
     CDS             6260..7978
                     /gene="argS"
                     /locus_tag="WGQ85_001643"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0751023.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8073..8448
                     /locus_tag="WGQ85_001644"
                     /pseudo
     CDS             8073..8448
                     /locus_tag="WGQ85_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8544..9962
                     /locus_tag="WGQ85_001645"
     CDS             8544..9962
                     /locus_tag="WGQ85_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0751024.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2326 a         2973 c         2545 g         2246 t
ORIGIN      
        1 ttaatccact ataaatttaa tccactatat tttttgttcc aaagtcaaaa tatgccgtcc
       61 gaacattcgg gcggcagaca aaacggcact gcccgataaa ggcagtgccg ttgtccgttt
      121 caaaccgtga aacatcagcc caaattaaag gctttatgca ataccctggt tgccagttcc
      181 atgtattttt catcaatcaa tacggaaact ttgatttcgg aggtggaaat catttggatg
      241 ttgataccct cttcggcgag cgtgcggaag attttggcgg ctacaccgac gtgcgaacgc
      301 atacccaaac cgactgcgga gactttgcat acggtgtcgt cgccatcaat agaagccgcg
      361 ccgatactgt cttggcgttc cgacaggatt tccaaagtct gcttgtaatc gccgcgcggt
      421 acggtaaagg aaaaatcggt tgtgccttcg ctgccgacat tttggataat catatcgact
      481 tcgatgttgg catcggcgat cgcgcctaaa atctgatagg caacgccggg tttgtcgggt
      541 acgccgcgca cgttgatgcg ggcttggttt ttatcgaatg cgataccggt tacggcagct
      601 ctttccatgt tgtcgtcctc ttcaaaggta attaaggtgc cgttgccgcc gtcttgcagg
      661 ctgctcagta cgcgcaggcg cactttgtac tttccggcga attctactga acggatttgc
      721 aagactttcg agccgaggct tgccaattcg atcatttctt caaatgtaac cgtatccatg
      781 cggcgcgctt cgggtacgac gcgggggtcg gttgtgtaaa cgccgtctac gtcggtatag
      841 atttggcatt cgtccgcttt gagggctgcg gcaagcgcaa cggcggaagt gtcggaaccg
      901 ccgcgtccga gcgtggaaat atcgccttca ctgctgatgc cttggaagcc ggcgacgatg
      961 acgactttgc cggcggcaag gtcggcacgc attttttcgt catcaatgct ttcgatgcgg
     1021 gctttggtgt gggcggtatc ggttttgagg gcgacctgcc agcctgtgta gcttttggca
     1081 tccacgccga tgtctttcaa tgccatcgcc aaaaggccga tggttacttg ttcgccggtg
     1141 gacaaaacca cgtccaattc gcgcggatcg gggtgctcct gcatttcgtg cgccaacgcg
     1201 accaggcggt tggtttcacc gctcatggcg gatacgacga cgacgatgtc gtgtccttcg
     1261 gcgcgggctt tggcgacacg tttggccacg tttttaatgc gttcgggcga gcctacggat
     1321 gtgccgccgt atttatgtac gattaacgcc atgtttcgtg ctttcttgtg tagggttggt
     1381 cgggcagcat atttgctgga aaagcggcta ttattactat tttttacatg aagttcaaga
     1441 acggactgcg ctttcccgcc tgccgtttga cagcggtcag cgaaaaacct gttctttcag
     1501 atttttgaca aaatgccgtc tgaacggttt tcagacggca tacggacagt aattaggcgg
     1561 cggataacgc attttgctga taacgcacca gctctcttat gcctttttcc gccagtccga
     1621 tcagcttgcc caattcttcc aaactgaatg gcgcgccttc cgccgtacct tggatttcga
     1681 tgattttccc cgatgcggtc ataacgatat ttacatcact gtcgcaaccg gaatcttcag
     1741 gataatccaa atctaaaagc ggcactccgc cgactatgcc tgcggatacg gcggcaacgg
     1801 cctcgcggat gggattttcg gtcaatatgc cgtctgaaat cagctttccg acggcaatgt
     1861 gcagtgccac aaatgcgccg gtaatcgaag ccgtgcgcgt accgccatcc gcctgaatca
     1921 catcgcagtc aatcaagatt tgtcgttcgc cgagtttttc catatccacg accgcgcgca
     1981 gcgaacgccc gataaggcgt tggatttctt gtgtgcgccc ggactgtttg cccgccgaag
     2041 cttcgcggcg catccgggaa gcggtggacg caggcagcat cccgtattcc gccgttaccc
     2101 agccttggtt tttaccgcgc agaaacgatg ggacattttc atccacggaa gcggtacaaa
     2161 tcactttgct attgccgcat tcaataaggc acgaaccgtc cgtatgcggc aggaaatgag
     2221 gggtgatttt gatatcgcgc aggctgtcgg cggcgcgtga ggtgcggatg taatcaggca
     2281 tactgccctc ccgttaaaaa cagataaatt aaaaagcctt aaatatgaaa aatcacattt
     2341 aaggccttca aactgaaaat ttctacgcct cttcggcttt gctgcggata atcaaaagcg
     2401 gcaggtggct ttggcgcatt accgtttcag caaaactgcc cattaaaagg tgcatcagcc
     2461 cggtacgtcc gtgcgtaccc aacaccagca ggtcggcacc gttttcatcg gcataatcaa
     2521 ccaaatcctg cgccatttca cgcgcaccct tattggcaac cagcaggtgt ttgacggtat
     2581 tttccacacc cagttcccgg gcggtgcgct cggcggcatc caaaacttcg ttgccttgcg
     2641 cgacggcggc ggcttcgtag ctttcgtgtt gcaaaaattc gggggcgagc gccatatatt
     2701 cggcaggatt ggcaacgtgt accaaagtca gacgcgcacc gttgaccccg gcaagctcgg
     2761 cggcatgttt cagggcattg atggacgttt cactgccgtc aacggcaaca accaaatgtt
     2821 tgtacatatc gtattctcct tttgcaccgc ctcgcggtgc cctcttgtcg gatgggcgca
     2881 gggacagttt gcgctgtttc attatagacc cgccgtcggg ctttatacaa cagccgaaca
     2941 gcccgaccgc tttccagtat aatatgccgc ttccgtgcag tcaggcattt tttgccggct
     3001 ttcgttcact ttttgatttg acgcaatctt gcaggattcg accatgtcca acaacgcttt
     3061 gacctcttcg cgacgattcg gcggcatcgc cagactctac ggagactccg ccttggcgca
     3121 cttttcacag gcacacgtct gcgtagtcgg cgtgggcggt gtcggctcgt gggcggtcga
     3181 ggctttggcg cggacgggca tcaggcattt gactttgatt gatttggaca acgttgccga
     3241 atcgaatgtc aaccgccagc tgcacgccct gaccggcgac ttcggcaaag caaaagttac
     3301 cgccttgcgc gaacgcatta cacaaatcaa tccgcaatgc gaagtatttg aaattgaaga
     3361 tttcgttacc gaagacaatt tgccggaata cttcggaaaa ggttttgatt tcgtcatcga
     3421 cgcgatcgac caagtgcgcg tcaaagcggc aatggcggct tattttgtgg aatgcaaaca
     3481 accgtttgtc ctcagcggcg gcgcgggcgg acaaaaagat ccggcgttga tccaaaccgc
     3541 cgatttgagc cgcgtaaccc acgacccgct gcttgccaac ctgcgctaca ccttgcggaa
     3601 acgctacggc ttcagccgcg atacgaaagc caaaatgcgc gtgccgtgcg tgttctcaac
     3661 cgaaaacatc acgccgccgc aatcgggcgc ggcgtgttct accgatgccg ctccgcaagg
     3721 cttgtcgtgc gccggctacg gtgcaagcat gctcgttacc gcttcgttcg ggctatattg
     3781 cgcacaggcg gcggtggaac acatcgcggg caaaaaataa gcaatgccgt ctgaaacagg
     3841 attcagacgg catttgaaca aacgcggcaa tcaaactgtc ggttcagaaa aaccaacatc
     3901 ggcacgccta aaaccgtatt cacaatctac tccttttcaa aacatttgca tttaaaagcc
     3961 gttataatgc cgtctgaaca tctgcccgac cacattatgc gtgaatgccg gcagattgtt
     4021 ttcttttgta aacttatatt aaaatccact taccgattca cgccatgctg cccatccctg
     4081 ccccatctgc accatccgag cacactgtcg catgggtatt cggtcaaccc gttaccgatt
     4141 tgccgcagga tttgtttatc ccgcccgacg cgctgaaagt cgtattgggc agttttcaag
     4201 gccctttgga tctactgctg tatctgatcc gcaaacagaa tatcgacgta ctggatattc
     4261 cgatggtaaa gattaccgag cagtatctgc actacatcgc ccaaatagaa acctatcagt
     4321 ttgatttggc ggcggaatat cttttgatgg cggcaatgct gattgaaatc aaatcgcgcc
     4381 tgctgctgcc gcgtaccgaa accgtcgaag acgaagaagc cgacccgcgt gccgagttgg
     4441 tgcgccgcct gctggcttac gaacagatga agctggcggc gcagggtttg gacgcgctgc
     4501 cccgagccgg acgggatttc gcgtgggctt acctgccgct ggaaattgcc gtcgaagcca
     4561 agctgcccga agtctatatt accgacttga cgcaagcgtg gctgggtatt ttgtctcggg
     4621 caaaacacac gcgcagccac gaagtaatca aagaaaccat ctccgtgcgc gcgcaaatga
     4681 cggcaatcct gcgccgtttg aacggacacg ggatatgcag gtttcacgac ctgttcaatc
     4741 ccgaacaggg cgcggcttac gtggtcgtca acttcatcgc actgttggag cttgccaaag
     4801 aaggtttggt cggaatcgta caggaagtcg gtttcggaga aatccgaatc agcctcaatc
     4861 atgagggggc gcattcagac ggcatttccg gcacacgggg cgggcgcgat gtgttctaat
     4921 acgccccaag ccgccaccaa aaatcgggag acacgccata tgaccggcat catacattcg
     4981 ctgcttgaca ccgacctcta caaattcact atgctgcaag tggttctgca ccagtttccg
     5041 cagacgcaca gcctttacga attccgctgc cgcaacgcct cgaccgtcta tccgcttgcc
     5101 gacatcaggg aagacttgga agtcgaactc gacgcgctct gccaactacg cttcacccac
     5161 gacgaactcg actacctccg tagcctgcgt ttcattaaaa gcgactttgt cgattatctc
     5221 gaactcttcc agctccaacg ccgctttgtc gaagtcggca cagacgataa aggccgtctg
     5281 aacatccgca tcgaaggtcc gatgatacag gcgatgtttt ttgaaatctt catcctcgcc
     5341 attgtcaacg aactttactt ccgccgcctg gaaacccctg cagtcataga agaaggcgaa
     5401 cgccggcttc aagccaaagc cgcgcgcctc aaagaaatcg ccgccgcaca aaaccccgac
     5461 gaaccgccct tcctgatttc cgacttcggc acgcgccgcc gctacaagct cgcgtggcag
     5521 gaacacgtca tccgcaccct gctcgaagcc gccccaagca tcgtgcgcgg caccagcaat
     5581 gtctttctcg ccaaaaaact cggcatcacc cccatcggca ccatggcgca cgagttcctc
     5641 caagccttcc aggccctcga cgtacgcctg cggaatttcc aaaaggccgc gctcgaaagc
     5701 tgggtgcacg aataccgggg cgatttgggc gttgccctga ccgacgtggt cggtatggat
     5761 gccttcctgc gcgatttcga cctctatttc gccaaacttt tcgacgggct gcgccacgac
     5821 agcggcgacc cttacgtttg gggcgacaaa gcctacgccc actatcaaaa gctcaaaatc
     5881 gacagccgca ccaaaatgct gaccttctcc gacgggctgg acatcgaacg ctcttgggca
     5941 ttacaccaat atttcaaaga ccgcttcaaa accggcttcg gcatcggcac caacctgacc
     6001 aacgatatgg ggcatacgcc tttgaatatc gtcttgaaac tggtcgaatg caacgggcag
     6061 tccgtcgcca agctgtccga ctctccgggc aaaaccatga ccaacaacag caccttcctc
     6121 gcctacctgc gccaagtgtt cgacgtaccc gaacccgaaa cgccgtaaac cggcagaaaa
     6181 agcgcacaat tcctgtttct gccgcataaa atcttttaaa ataccgcctg atttgaattt
     6241 aaccgaaaga ccgaacttca tgaacctaca tcaaaccgtc gaacacgaag ccgccgccgc
     6301 ctttgccgcc gcaggcatcg ccggcagccc cgttgttttg cagccgacca aaaatgccga
     6361 acacggcgat ttccaaatca acggcgtgat gggtgcggcg aaaaaagcca aacaaaaccc
     6421 gcgcgaactg gcgcaaaagg tcgccgacgc attggcaggc aacgccgtca tcgaaagcgc
     6481 ggaagtcgcc ggtcccggtt ttatcaacct gcgcctgcgc cccgaatttc tcgcgcaaaa
     6541 cattcagacg gccttgaacg acgcgcgttt cggcatagcg aaaaccgaca aaccgcaaac
     6601 cgtcgtcatc gactattcct cgcccaatct ggcgaaggaa atgcatgtcg gtcacctgcg
     6661 ttccagcatc atcggcgaca gcatttcgcg cgtgttggca tttatgggca acaccgtcgt
     6721 ccgtcaaaac cacgtcggcg actggggtac gcagttcggc atgttggtcg cttatttggt
     6781 cgagcagcaa aaagacaatg ccgcgtttga gctggcggat ttggagcagt tttaccgcgc
     6841 cgccaaagtg cgctttgacg aagatcctgc cttcgccgac accgcgcgcg aatacgttgt
     6901 gaagctgcaa ggcggcgatg aaaccgtttt ggcattgtgg aaacagtttg tcgatatttc
     6961 gctctcgcac gcccaagccg tttacgacac gctgggcttg aagctgcgcc ccgaagacgt
     7021 ggcgggcgaa tcgaagtaca acgacgattt gcagccagtg gtcgatgatt tggttcaaaa
     7081 aggtctggcg gtcgaagacg acggcgcgaa agtcgtattc ttggacgagt tcaaaaacaa
     7141 agaaggcgaa cccgccgcgt ttatcgtgca aaaacaaggc ggcggcttcc tttatgcttc
     7201 caccgacttg gcgtgtttgc gctaccgtgt cggcacgctt catgccgacc gcctgctgta
     7261 cgtcgtcgac caccgccaag ccctgcactt tgagcaactc tttaccactt cccgcaaagc
     7321 aggctatctg ccggaaaacg tcggcgcggc atttgtcggc ttcggcacga tgatgggcaa
     7381 agacggcaaa ccgttcaaaa cacgcagcgg cgacactgta aaactggtcg atctgctgac
     7441 cgaagccgtc gaacgcgccg ccgctttggt gaaagagaaa aatcccgaat tgggcgcgga
     7501 cgaagccgcc aaaatcggca aaaccgtcgg catcggcgcg gtcaaatacg ccgacttgag
     7561 caaaaaccgc accagcaatt atgtgttcga ctgggacgct atgctttcgt ttgaaggcaa
     7621 caccgccccc tacctgcaat atgcctacac ccgcgtgcaa agcgtgttcc gcaaagccgg
     7681 tgaatgggac gcaaccgcac cgactgtttt gagcgaaccg ctggaaaaac agcttgccgc
     7741 cgagctgctg aaattcgaag acgtgctgca aagcgtggcg gacacggcgt atccgcacta
     7801 cctcgccgcc tacctctatc aaattgcgac cctgttcagc cgcttctacg aagcctgtcc
     7861 gatactcaaa gccgaaagcg caagccgcaa cagccgcctg caactggcga agctgaccgg
     7921 cgacacgctg aaacaaggct tggatttgct gggcatcgat gtgttggacg taatgtaaaa
     7981 ccgcaccgcc cgattgcgga caacagcctc gccatcctta tccgaatctg aaaaaagcgg
     8041 cgcgatacac cgtatccgcc gcccctccca aaatgcgaaa caaacaaacg ccaagcaagc
     8101 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaaaaaatt
     8161 ataaccccct tcctgccgac gcacgcactt tccgcgcggc gcattcccct tttcccgccc
     8221 ctcaaatccg ccttttcttc aggcagggtt tcagcccgcc tcttttccct gttttccttt
     8281 ccccgacacg cgtgcgcgcc ccctgccgca ctgtgctgca ctttcgcgcc cggacggcat
     8341 cgttccgcca tccggttctc tgttttaaat acccctgttt cagaaagaaa tgcagatgtt
     8401 tcaacacaca ggacgacaca taaagcaccg ccctatgtgt tgccctgatt tggaaggggt
     8461 tacgcctccc aaataaagtc tgatcctgcc gccccgaagg acagatgtcc gagtggcgaa
     8521 gtttcaaccg aaaaggaaat acgatgaata ttcacaccct gctctccaaa caatggacgc
     8581 tgccgccatt cctgccgaaa cggctgctgc tgtccctgct gatactgctg gcccccaatg
     8641 caatgttttg ggttttggca ctgctgaccg ccaccgcccg cccgattgtc aatttggact
     8701 atcttcccgc cgcgctgctg atcgccctgc cttggcgttt cgtcaaaatt gccggcgtat
     8761 tggcgttttg gctggcggtt ttgtttgacg ggctgatgat ggtgatccag ctcttccctt
     8821 ttatggacct catcggcgcc atcaacctcg tccccttcat cctgaccgcc cccgcccctt
     8881 atcagataat gaccgggctg ttgctgctgt atatgctggc gatgccgttt gtgttgcaga
     8941 aagccgccgc caaaaccgac ttccgacaca ttgccgcctg tgccgccgtt gtggcggcag
     9001 ccggctattt cactggccat ttgagttact acgaccgggg gcggatggcc aatatcttcg
     9061 gcgcaaacaa cttctactac gccaaaagtc aggcgatgct ctacaccgtc agccagaatg
     9121 ccgactttat taccgccggc ctggtcgatc ccgtcttcct ccccttgggc aatcaacagc
     9181 gtgccgccac gcatctgaac gagccgaaat ctcaaaaaat cctctttatc gtcgccgaat
     9241 cttgggggct gccggccaat cccgaacttc aaaacgccac ttttgccaaa ctgctggcgc
     9301 aaaaagaccg tttttcggtt tgggaaagcg gcagttttcc ctttatcggc gcgacggtcg
     9361 aaggcgaaat gcgcgaactg tgtgcctacg gcggtttgcg cgggttcgca ctgcgccgcg
     9421 cgcccgacga aaaatttgcc cgctgcctcc ccaaccgttt gaaacaagaa ggttacgcca
     9481 cctttgcgat gcacggcgcg ggcagttcgc tttacgaccg cttcagctgg tatccgaggg
     9541 cgggctttca agaaatcaaa accgccgaaa acctgatcgg taaaaaaacc tgcgccattt
     9601 tcggcggcgt gtgcgacagc gagctgttcg gcgaagtgtc ggcatttttc aaaaaacacg
     9661 acaagggact gttttactgg atgacgctga ccagccacgc cgactatccc gaatccgaca
     9721 ttttcaacca caggctcaaa tgcaccgaat acggcctgcc cgccgaaacc gacctctgcc
     9781 gcaatttcag cctgcacacc caattcttcg accaactggc ggatttgatc caacgccccg
     9841 aaatgaaagg cacggaagtc atcatcgtcg gcgaccatcc gccgcccgtc ggcaacctca
     9901 atgaaacctt ccgctacctc aaacaggggc acgtcgcctg gctgcacttc aaaatcaaat
     9961 aacaacaatg ccgtctgaac gcaccaacag ccttcagacg gcattttgca gacagaccga
    10021 cccttcaagc ccactttttt catcatctcc gataaattgc tttgtatagt ggattaacaa
    10081 aaaccagtac
//
LOCUS       DAWFIT010000073         9975 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFIT010000073 DAWFIT010000000
VERSION     DAWFIT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.073"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            54..581
                     /locus_tag="WGQ85_001646"
     CDS             54..581
                     /locus_tag="WGQ85_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0751025.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            669..1724
                     /gene="plsX"
                     /locus_tag="WGQ85_001647"
     CDS             669..1724
                     /gene="plsX"
                     /locus_tag="WGQ85_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0751026.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            1860..2111
                     /locus_tag="WGQ85_001648"
     CDS             1860..2111
                     /locus_tag="WGQ85_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0751027.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2362..2553
                     /locus_tag="WGQ85_001649"
     CDS             2362..2553
                     /locus_tag="WGQ85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751028.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            2573..2776
                     /locus_tag="WGQ85_001650"
     CDS             2573..2776
                     /locus_tag="WGQ85_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751029.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            2803..3765
                     /locus_tag="WGQ85_001651"
     CDS             2803..3765
                     /locus_tag="WGQ85_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0751030.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3812..4183
                     /locus_tag="WGQ85_001652"
     CDS             3812..4183
                     /locus_tag="WGQ85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0751031.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            4209..5135
                     /gene="fabD"
                     /locus_tag="WGQ85_001653"
     CDS             4209..5135
                     /gene="fabD"
                     /locus_tag="WGQ85_001653"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0751032.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            5274..7139
                     /gene="msbA"
                     /locus_tag="WGQ85_001654"
     CDS             5274..7139
                     /gene="msbA"
                     /locus_tag="WGQ85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0751033.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKQVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            7239..8804
                     /gene="guaA"
                     /locus_tag="WGQ85_001655"
     CDS             7239..8804
                     /gene="guaA"
                     /locus_tag="WGQ85_001655"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0751034.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            8866..9612
                     /gene="fabG"
                     /locus_tag="WGQ85_001656"
     CDS             8866..9612
                     /gene="fabG"
                     /locus_tag="WGQ85_001656"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0751035.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(9874..9975)
                     /locus_tag="WGQ85_001657"
                     /pseudo
     CDS             complement(9874..9975)
                     /locus_tag="WGQ85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2536 a         3073 c         2380 g         1986 t
ORIGIN      
        1 ggtttttgtt aatccgctat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
       61 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
      121 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
      181 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
      241 acgacagcga caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctcgacc
      301 ccgttccaga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
      361 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
      421 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
      481 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
      541 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
      601 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
      661 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
      721 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
      781 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
      841 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
      901 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
      961 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaaa
     1021 ccattcccgg catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg
     1081 ttaccctcgc cctcgacctt ggcgcgaacg tcgactgcac gcccgaacag ctcgcccaat
     1141 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgtg
     1201 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     1261 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     1321 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     1381 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     1441 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     1501 tcgatccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa
     1561 gccacggcgg cacagacgaa accggtttcc gctacgcact cgaagaagcc taccacgaag
     1621 ccaagtccgc cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactcg
     1681 aaactgccaa agccgtccaa aacgaaaatg ccgacggtct gtaacacaca cgatgccgtc
     1741 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
     1801 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa
     1861 tgaagacatt agaaaaacgg atgaaagctc tagacaaaca gatgatgaag ttcggaaaat
     1921 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
     1981 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
     2041 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
     2101 cgtccgatta accaataaag ccaatgggtt gataaacatg aaaacatcga cggtcgtttt
     2161 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
     2221 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
     2281 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tatctatttt
     2341 gaaaaaggat attatttgcc aatgattcaa acaattttag aagacgggga tattgaagtc
     2401 aaaaacctta aaacagaaaa ttacagcggg aaggcaaggg aaatcttggg agattcctac
     2461 cctattgagc atatctctaa aaatatttct acaattcaaa atataatttc tgaatttatg
     2521 atacaaaaac aaacaccggc accaataatt tgatggaaaa ccaacaatca aaatgccgtc
     2581 tgaagcatgt ttggttttca gacggcattt ccttcgctta aaacagcgta tcggcaaccc
     2641 cgccctgcct gtccacggca atctgcatct gaaaaccatc tgtatcccaa accacacccc
     2701 catccctgtt tccgtcattt gcaccctgtc cgtattgggc aatcatcggt ttttcgctta
     2761 caatagccga atctgaacca actatccaaa aaggccgttc ccatgcagta tgccaaaatt
     2821 tccggcacgg gcagctatct tcccgccaac cgcgtcagca atgacgacct tgcccaaaaa
     2881 gtggatacct cggacgagtg gattaccgcg cgcacgggca tcaaattccg ccatattgca
     2941 gccgaaaatg agaaaaccag cgatcttgcc gccgaagcag cccgccgcgc actggatgca
     3001 gccggattag acagcggcga aatcgattta attattgtgg cgacggcaac gccggatatg
     3061 cagtttcctt ctaccgccac catcgtccaa caaaaactgg gcatcaccaa cggctgcccc
     3121 gcgtttgacg tacaggcggt gtgtgcgggc tttatgtacg cgctgaccac ggcaaacgcc
     3181 tacatcaaaa gcggcatggc aaaaaacgcg ctggtcatcg gcgcggaaac tttcagccgc
     3241 atcgtcgatt ggaacgaccg cacaacctgc gtattgttcg gcgacggcgc gggcgcggtg
     3301 gttttgagcg cggcggacaa accgggcatc atccacagca aactcaaagc cgacggcaat
     3361 tatctgaaac tcttaaacgt ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg
     3421 tacatttcga tggacggtcc cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc
     3481 gccgatgacg ttatcgaaga agccggttat accgccgctc aaatcgactg gatcgtgccg
     3541 catcaggcaa accgccgcat tatcgaatcg accgcgaaac atttaggttt gagtatggac
     3601 aaagtcgtcc tgaccgtcca agaccacggc aacacatccg ccgcatcgat tccgctggct
     3661 ttggatacgg gcatccgcag cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc
     3721 atcggcggcg gtttcgcgtg gggcgcggtg ctgttgcaat attgaacccg atgccgtctg
     3781 aaacaggctt tcagacggca tttcccatat catgaagcgg caggctttct tcaaaccaat
     3841 tgcgtgtgcg gcatttctgt ctgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta
     3901 cgcaatattg tccctctatg cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg
     3961 gggaaaacgc cgcattcccg aacaccgcct gctgctgcct gccttgttcg gcggttgggc
     4021 gggcgcatac ttgggcagca ggatattcag gcataaaacg gcgaaaaagc gttttgttgt
     4081 gctgttccgt ctgactgttt cgggcaatgt cctggcgacc tgcatcctga ttgattattt
     4141 cgttccgccc gaactttttg tcaaactcgg gcaacatctc tgacaatcca accaaacaaa
     4201 ggaataccat gtcttttgca ttcttctttc ccggacaagg ttctcaaagc ctcggcatga
     4261 tgaacggctt tacagggcag gccgtcgtca aaaacacatt tgacgaagcc tccgccatat
     4321 tggggcagga cttgtgggcg atgattaacg gcagcgatgc cgaaatcatc ggtcaaaccg
     4381 tcaataccca acccatcatg ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag
     4441 cgggtggcaa aacgcctgcc gccgttgccg gacacagcct cggcgaatat accgcgcttg
     4501 tcgccgccgg cgcattggat tttgccaacg cggtcaaact cgtgcgccta cgcgcagaac
     4561 tgatgcagtc cgccgtaccg caaggcgtgg gcgcaatggc ggcaattttg ggtttggaag
     4621 atgaacaggt aaaacaaatt tgcgccgaat ccacacaagg cgaagtggtt gaagccgtca
     4681 acttcaactc gcccggacaa gtcgtgattg caggcaatac cgccgccgtc gagcgcgcta
     4741 tgcttgccgc caaagaagcc ggcgccaaac gcgccctgcc gctgcccgtg tccgtacctt
     4801 cccattgcag cctgatgaaa cccgccgccg acaaactcgc cgaagccctg aaaaccgttg
     4861 aaatcaagca gccgcaaatc cgcgttatcc acaacgccga cgttgccgcc tacgatgatg
     4921 ccgacaaaat caaagacgcg ctcgtccgcc agctttacag ccccgtacgc tggacggaaa
     4981 ccgtcaacgc cctcgtttca gacggcattg ccgaatccgc cgaatgcggc ccgggcaaag
     5041 tgttggcggg cttggcaaaa cgcatcaaca aagccgccgc gtgcagcgca ctgaccgatg
     5101 ccggacaggt tgccgccttt atcgaaacgc actgacttcg ttccgcaaaa agcagcctgc
     5161 atgcctcagg ctgcttttca cgtccgaacg gcaaacagcc gcatatttgc gctataattg
     5221 atgacgacca aaccaccgac agcggctgcc gttgcagttc ccgccccacc gacatgatag
     5281 aaaaactgac tttcggactg tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg
     5341 cgtacgtccg cccctacaaa atccgcatcg ttgccgccct gattgccatt ttcggcgttg
     5401 ccgccaccga aagctacctt gccgccttca tcgcccccct gattaaccac ggcttttccg
     5461 cacctgccgc gccgcccgag ctgtctgccg ccgccggcat catttccacc ctgcaaaact
     5521 ggcgcgaaca gtttacctat atggtttggg ggacggaaaa caaaatctgg accgtcccgc
     5581 tctttctcat cattctcgtc gtcatccgtg gcatctgccg ctttaccagc acctatctga
     5641 tgacttgggt ctccgtgatg accatcagca aaatccgcaa agatatgttt gccaaaatgc
     5701 tgaccctttc ctcccgctac catcaggaaa cgccgtccgg caccgtactg atgaatatgc
     5761 tcaacctgac cgaacagtcg gtcagcaacg ccagcgacat cttcaccgtc ctcacgcgcg
     5821 acacgatgat tgttaccggc ctgaccatcg tcctgcttta cctcaactgg cagctcagcc
     5881 ttatcgtcgt cctgatgttc cccctgctct ccctgctctc gcgctactac cgcgaccgtc
     5941 tgaaacaagt catttccgac tcgcaaaaaa gcataggcac gatgaacaac gtgattgccg
     6001 aaacccatca gggacaccgc gtcgtcaagc tgttcaacgg gcaggcgcag gcggcaaacc
     6061 ggttcgacgc gatcaaccgc accatcgtcc gcctcagcaa aaaaatcacg caggcaacgg
     6121 cggcacattc cccgttcagc gaactaatcg cctcgatcgc cctcgccgtc gtcatcttca
     6181 tcgccctgtg gcaaagccaa aacggctaca ccaccatcgg cgaatttatg gcattcatcg
     6241 tcgcgatgct gcaaatgtac gcccccatca aaagccttgc caacattagc atccctatgc
     6301 agacgatgtt cctcgccgcc gacggcgtat gtgcatttct cgacaccccg cccgaacagg
     6361 acaaaggcac gctcgcaccg cagcgtgtcg aagggcgcat cagcttccgc aacgtcgatg
     6421 tcgaataccg ttcggacggc atcaaagccc tcgacggctt caacctcgac atcagacaag
     6481 gcgaacgcgt cgccctggtc ggacgttccg gcagcggcaa atccaccgtc gtcaacctgc
     6541 tgccccgctt tgtcgaaccg tctgccggca acatctgcat agacggtatc gacatcgccg
     6601 acatcaaact cgactgcctg cgcgcccaat ttgccctcgt ctcccaagac gtattcctgt
     6661 ttgacgacac cctgtttgaa aacgtccgat atagccgtcc cgacgcgggc gaagccgaag
     6721 tcctatccgc cctccaagcc gctaacctgc aaagcctgat cgacgcatca ccgctcggac
     6781 tgcaccagcc catcggatcg aacggcagca acttatccgg cggacagcgg caacgcgttg
     6841 ccattgcccg cgccattttg aaagacgcgc cgatattatt attggacgaa gccaccagcg
     6901 cattggacaa cgaatccgaa cgcctcgtcc aacaggcgct cgaacgcctg atggaaaacc
     6961 gcaccggcat catcgtcgcc caccgcctga ccaccatcga aggggccgac cgcatcatcg
     7021 taatggacga cggcaaaatc atcgaacaag gcacacacga acaactgatg tcccaaaacg
     7081 gctactacac gatgttacgc aatatctcaa acaaagatgc cgccgtccgg acggcataaa
     7141 caaaatgccg tctgaaatgg tacaatcgcc ccgacccttt cagacggcat catatccgcc
     7201 gacccatccg attatcttca atcactgtaa aacccattat gacccaagac aaaatcctca
     7261 tccttgactt cggttcgcaa gttacccagc tcatcgcccg ccgcgtgcgc gaagcccacg
     7321 tttactgcga gctgcattcc ttcgatatgc ctttggacga aatcaaagcc ttcaacccca
     7381 aaggcatcat cctttccggc ggccctaatt ccgtttacga atccgactat caagccgata
     7441 ccggtatctt tgacttgggc attccgattt tgggcatttg ctacggcatg caatttatgg
     7501 cgcaccactt gggtggtgaa gtgcaacccg gcaaccagcg cgaattcggt tacgcgcaag
     7561 tcaaaaccat cgacagcgag ctgacacgcg gcattcaaga cgatacgcca aacacgctcg
     7621 acgtatggat gagccacggc gataaagttt ccaaactgcc taccggtttc accgtcatcg
     7681 gcgacacgcc gtcctgcccg attgccatga tggaaaacgc cgaaaaacaa ttctacggca
     7741 tccaattcca ccccgaagta acccacacca aacaaggccg cgccctgttg aaccgctttg
     7801 ttttggatat ttgcggcgca caaccgggct ggacaatgcc gaactacatc gaagaagccg
     7861 ttgccaaaat ccgcgaacaa gtcggcagcg acgaagtgat tttaggcttg tccggcggcg
     7921 tggactcttc cgtagccgcc gcgctgatcc accgcgccat cggcgaccaa ctgacctgcg
     7981 tgttcgtcga tcacggtttg ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg
     8041 cccgcaactt gggtgtgaaa gtgattcacg tcgatgccga agggcagttt atggcgaaac
     8101 tcgccggcgt taccgaccct gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag
     8161 tatttgatgc cgaagagaaa aaactcacca acgccaaatg gctggcgcaa ggcacgattt
     8221 accccgacgt aatcgaatcc gcaggcgcga aaaccaaaaa agcccacgcc atcaaatccc
     8281 accacaatgt cggcggcctg cctgaaaaca tgaagctcaa gctgcttgag ccgttgcgcg
     8341 acttgtttaa agacgaagtg cgcgaactgg gcgttgcttt gggtctgccg cgcgaaatgg
     8401 tgtaccgcca tccgttcccc ggccccggtt tgggcgtgcg catcttgggc gaagtgaaaa
     8461 aagaatacgc cgacttattg cgtcaagcag acgatatttt catccaagaa ttacgcaaca
     8521 ccaccgacga aaacggcaca tcttggtacg acctgaccag ccaggcattt gccgtattcc
     8581 tgcctgtgaa atccgtcggc gtaatgggcg acggacgcac ttacgactac gtcgtcgcac
     8641 tgcgcgcagt catcaccagc gacttcatga ccgcacactg ggcagaactg ccttactcac
     8701 tgctcggacg cgtgtccaac cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt
     8761 acgatgtcag cggcaaaccg cctgccacca tcgagtggga ataaccagca aacagggctg
     8821 caccgtccgg cgcagtcttt cgattatcgg aaaaaaggaa aaaatatgag cacacaagat
     8881 ttaagcggca aaatcgcttt ggtaaccggc gcatcgcgcg gtatcggtgc agcaattgcc
     8941 gacacactgg cggcagccgg tgccaaagtc atcggtacgg cgaccagtga gagcggtgcg
     9001 gcggcgatta gcgagcggtt ggcgcaatgg ggcggcgaag gccgcgtatt aaattccgcc
     9061 gaacccgaaa ccgtcgaaaa cctgattgcc gacatcgaaa aaacgttcgg caaactcgat
     9121 attctggtca acaacgccgg catcacccgc gacaacctcc tgatgcgcat gaaagaagaa
     9181 gagtgggacg acatcatgca ggtcaacctc aaatccgtgt tccgcgcttc taaagccgtc
     9241 ttgcgcggca tgatgaagca gcgcgccggc cgtatcatca acatcacatc cgtcgtcggc
     9301 gtgatgggca atgccggtca aaccaactat gccgcggcaa aagcaggctt aatcggtttc
     9361 tccaaatcca tggcgcgcga agtcggcagc cggggcatta ccgtcaactg cgtcgccccc
     9421 ggctttatcg ataccgatat gacccgcgcc ctgccggaag aaacccgcca agcctttacc
     9481 gcccaaaccg ccttgggcag attcggcgac gcgcaagaca ttgccgatgc ggttttgttc
     9541 ctcgcttccg atcaggcgaa atacattacc ggccagacgc tgcacgtcaa cggcggcatg
     9601 ctgatgccct gacggacagc cgtgccgaca atgccgtctg aagccctttc agacggcatt
     9661 tgcattccca aacaaaatga acacacacca caccctgccc tgcccctgcg gttcaggcac
     9721 aagctgagac ctttgcaaaa ctcctttccc tcccgacagc cgaaacccaa acacaggttt
     9781 tcgtctattt tcgccccaaa tatctcctaa ttttacccaa ataccccctt aatcctcccc
     9841 ggatacccga taatcaggca tccgggctgc cttttaggcg gcagcgggcg cacttagcct
     9901 gttggcggct ttcaacaggt tcaaacacat cgccttcaga tggctttgcg cactcactta
     9961 atcagtccga aatag
//
LOCUS       DAWFIT010000074         9955 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFIT010000074 DAWFIT010000000
VERSION     DAWFIT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.074"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(145..1587)
                     /gene="aldA"
                     /locus_tag="WGQ85_001658"
     CDS             complement(145..1587)
                     /gene="aldA"
                     /locus_tag="WGQ85_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0751036.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1622..1822)
                     /locus_tag="WGQ85_001659"
     CDS             complement(1622..1822)
                     /locus_tag="WGQ85_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751037.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1858..2220)
                     /locus_tag="WGQ85_001660"
     CDS             complement(1858..2220)
                     /locus_tag="WGQ85_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751038.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISV"
     gene            complement(2429..3346)
                     /locus_tag="WGQ85_001661"
     CDS             complement(2429..3346)
                     /locus_tag="WGQ85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0751039.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFVGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSVGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3518..4318
                     /locus_tag="WGQ85_001662"
     CDS             3518..4318
                     /locus_tag="WGQ85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0751040.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4366..5142
                     /gene="mlaE"
                     /locus_tag="WGQ85_001663"
     CDS             4366..5142
                     /gene="mlaE"
                     /locus_tag="WGQ85_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0751041.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAIDFVLTAWMFTD"
     gene            5194..5688
                     /gene="mlaD"
                     /locus_tag="WGQ85_001664"
     CDS             5194..5688
                     /gene="mlaD"
                     /locus_tag="WGQ85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0751042.1"
                     /translation="MKKNILEFWVGLFVLLGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5725..6315
                     /locus_tag="WGQ85_001665"
     CDS             5725..6315
                     /locus_tag="WGQ85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0751043.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTTRQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6372..6650
                     /locus_tag="WGQ85_001666"
     CDS             6372..6650
                     /locus_tag="WGQ85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0751044.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTGDAFERFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6647..7474
                     /locus_tag="WGQ85_001667"
     CDS             6647..7474
                     /locus_tag="WGQ85_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0751045.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7599..7961
                     /locus_tag="WGQ85_001668"
     CDS             7599..7961
                     /locus_tag="WGQ85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0751046.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIETRFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7958..8344
                     /locus_tag="WGQ85_001669"
     CDS             7958..8344
                     /locus_tag="WGQ85_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0751047.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8341..8829
                     /locus_tag="WGQ85_001670"
     CDS             8341..8829
                     /locus_tag="WGQ85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0751048.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8911..9078)
                     /locus_tag="WGQ85_001671"
     CDS             complement(8911..9078)
                     /locus_tag="WGQ85_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0751049.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9045..9425)
                     /locus_tag="WGQ85_001672"
     CDS             complement(9045..9425)
                     /locus_tag="WGQ85_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0751050.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9435..9561
                     /locus_tag="WGQ85_001673"
                     /pseudo
     CDS             9435..9561
                     /locus_tag="WGQ85_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9663..9878
                     /gene="rpmE"
                     /locus_tag="WGQ85_001674"
     CDS             9663..9878
                     /gene="rpmE"
                     /locus_tag="WGQ85_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0751051.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2339 a         2781 c         2475 g         2360 t
ORIGIN      
        1 gcggcttcgt ttaaatttaa tccgctatac aaagacaaaa acatcgacct cggtcatgac
       61 tgattgccga tgaagcaata aaaatgccgt ctgaacctga aaacgggttc agacggcatt
      121 ttctatcggg gttttaaagc ggcattaaat gtcggtttcc aaataaacga cttgggtttg
      181 cagatattct tccaaaccgt gtttgccgtc cgcaccgccg atgccggatt ttttccaacc
      241 ggcgtggaaa ccctgcatcg cttcaaagtt ttcgcggttg atgtaggttt cgccgaattg
      301 caggcggcgg gtaacgtaga aggcttcgtt taaattagtc gtataaacag aactggtcag
      361 accaaactcg caatcgttag ccagtgcgat gacttggtcg agcgtgtcga aagcggaaac
      421 gggcagaacg gggccgaagg tttcttcttt cataatgtcc atactgttgt cggtgtcggt
      481 cagcagggtc ggctcgaaga aatagccgcg tccttcggcg cgtttgccgc cgcaaaccag
      541 tttcgcacct tgtttgactg cccgttccac tttttcggca acggctttga ctgcgcgctc
      601 ttcaatcagc gggcccattt ccagcgcgcc tgcttcggct tcggcagggt tgccgtagcg
      661 tacgcctttc atggcggcag tcattttttc aatgaacgcg tctttcaggc tgctgtggac
      721 ataaacgcgc tcggcgcagt tgcagatttg accggtgttg ccgacgcgcg aagccaagat
      781 ggatttcacc gccaagtcca aatccgcgtc tttcaacacg atggcagggg ctttgccgcc
      841 gagttccaac gaaactttgg tgatgttggc ggaagcggct tccatcactt ggcggcctgc
      901 ttcgacggag ccggtcaggc tgaccatatc aacttgcgga tgggcggaca aggcattgcc
      961 gatttccgcg ccgggaccgt tcaccacgtt gaacacgcct gcgggcaatc cgaccgcatc
     1021 gacgatttcg gcgaagatgt ggcagttgat cggggttacg ctgctgggtt tgacgacgat
     1081 ggtgttgccc gttactaaag cggggcccat tttgcgggca atcaggaaga aggggaagtt
     1141 ccacggcaaa atgccggcaa tcacgcccag cggacgtttg aacaataaaa tattttcacg
     1201 cgggcggtcg ctttggatga tttcgccttc gtaacggcgt gcccattcgg cctggtaatc
     1261 gagataatcg gcggtgaaca tcacttccac gcgcgccaag tctttggttt tgccgccttc
     1321 ggcaacgatg gtgtcggtca gctcgtcggc gcgttcgcgt atgccttggg cgattttgcg
     1381 caaatacgcg ccgcgttcga ccgcaggcag acgctcccaa gccggttgcg ccgcacgcgc
     1441 cgccgctacg gcgcggtcaa cgtccgccct gccgcctttg ggttcgcggg cgatggcctc
     1501 ttcggtggac gggttcaata cgtcgcgcca ttcgccgttg aaatcgtttt caaagcgtcc
     1561 gttgatgtac atggccaatt gtttcatttc gggttctcca gttttgtagt cagatgtagt
     1621 cttagtttat tcccaaataa attgccgtac aagtttcttt tgaaatgttt agacgagaag
     1681 ctactatgtc tgcatattgt tctgctaatt tatattcact cgaatgcaac tgccgattat
     1741 tccaatctgc attcgcatca gtaaaagccc cagcattgcc gcctgtggca taaccaatcg
     1801 ctaaactgcc aacggtatcc acaacaggtt tcaggctgcc tgaatttctc taataaatta
     1861 aacacttatt ctatcagccg ttaatgtaac caaacaatta tcaagataag agtagtttac
     1921 taaatactca tcttcttcaa attgtatatt ttgtagggtt agatgattat tttttaattc
     1981 tgccgttatt tcatacttga aactatcatc tatttttcta aatccaatat ttgaagttga
     2041 tttatccagt atgtaatcat taaagatttc ataggataat tccacttcat aaaattgatt
     2101 ttcaaatatt ttgaatgggc agtatgatat aaaacaatgt agttcataac catttatttc
     2161 aatcaatact gcctcttcaa tggtgttatc taactttttg attttacatt tatataacat
     2221 tttatcttgc ctttagataa attgttttgt tttttggatt gttattcaaa gttctccaaa
     2281 agtcttttcc tgattttgta tgtatcgtgg taacactacc attttcattg gttccaacaa
     2341 aaataaattt agattgacct ttggaatcag tacccaaaaa ctgaacatat ccagacatgc
     2401 ggatttcggg ttttaaatca aatgcggctt accggttttc ttttttggtt tccgccggcg
     2461 tgctgccgaa atattgcctg aatgcctgag taaagctgga aacgtggcgg tagccgcata
     2521 aatacgcggt ttcgcctacg cttttgcccc cgttttgcaa cagatagagc gcgtgctgca
     2581 tttgtttgtg gtgcagccac tcgcttgccg taatgccgaa atggtcgcgc atacggcgtt
     2641 gcagcgtcct ttcgctgatg ttcagcgcgt ctgtcagccg gttgacttgg tgtgcgccgc
     2701 cgacaaacgc ggcattcagg gtgcggctga agtcttcaga cggcatagcg tctgcttccg
     2761 ccgtttgccc cgccgccggc ccgatgccgt ctgaaaccgt gtcccacaag tccgacagca
     2821 gccgcaaaac gtccgcctcg cggcgcaatg tttcgcccaa atgccccttt gggacggctt
     2881 tcaggcagga tgccgccaag ccgcgcaggt tcgggggcaa atcccatatc ctgaccggtt
     2941 cgcggtaaag caggggtgcg aaacgcgcgt attccggacg cagcagccat tgttccatac
     3001 ctttaatggt cattttgacc gttttgccgc ctcggtaaag atagcggctg aacaggactt
     3061 cttccccgac agcaattagg acaatcttgc cgccgtccgc atcgatttgg aagcggcagc
     3121 ggttgatgcc gaagtccaaa ctgccttcca gcaagagcac gaacgacaca taaggttcgg
     3181 cgaggcggct gctgcaaaaa tcacaccgtg ccgttaccgt gccgccgtgg atggaaatgc
     3241 cgttggacaa agtatcgaaa cggtagctgc cctccacata gctgccgccg ccgcttttga
     3301 ccagattgac gaaccggctg ctgttcaaat gttctgcttt ttccatagtt ttctgataaa
     3361 ggttatcatt tgaaagataa catttttcgc cacagcaaac aatccgccgc cacaacggca
     3421 ttttcacaac acttgccggc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct
     3481 taaaatacac gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga
     3541 aatgaaagac gtcgcttttg cgtatggcga ccgcccgatt ctgaacgaca tcaatttcag
     3601 cattccgcaa ggcaattttg ccgccgtgat gggcggttcg ggcagcggca aaaccacgct
     3661 gatgcggctg attacagggc agattcgtcc gcagtccggg caggttttga ttgaagggcg
     3721 ggatttgtcg ggcttttcgg ctgacgaact ctacgaacac cgccgccgta tgggcgtatt
     3781 gttccaacac ggcgcgctgt ttactgattt gtctgtattt gacaatatcg cttttccgat
     3841 gcgcgaactg acgcagctgc cggaagcggt gattcgagat ttggttttgt tgaaactgaa
     3901 cgcggtcggt ctgcgcggtg tagaaaacct gatgccgtcc gagttgtccg gcgggatgtc
     3961 gcgccgcgtc gcgcttgccc gcacgattgc gctcgacccc gaaattatgt tgtacgacga
     4021 accgtttacc ggtctcgatc cgatttcctt gggcgtgatt gcccacttaa tcagccgcgt
     4081 caacaaggct ttgcgttcga ccagtattat ggtaacgcac gacattgaaa aatctttgga
     4141 aatcgtcgat caggtgattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca
     4201 ggaaatgcgc gaactggatt cgccttgggt gcgccagttt gtcggcggct tggcagacgg
     4261 tcccgtagca taccgctatc cggcgcaaac gtcgttgcag caggacctgt tgggataaat
     4321 gagttgatgc cgtctgaaac cgttgaactt tacacataga aatttatgaa ctttatccgt
     4381 tccgtcgggg cgaaaaccct cggccttatt caatccctcg gcagtatcac gctgtttctg
     4441 ctgaacattc tggcgaagtc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa
     4501 gtgtatttcg ccggcgtgct gtcggtgttg attgttgccg tttcggggct gtttgtcggt
     4561 atggtcttgg gtttgcaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg
     4621 ggctatatgg tcgcggcttc gctgttgcgg gaactgggtc cggtgttggc ggcgatcctg
     4681 tttgccagca gcgcgggcgg tgcgatgacc agcgaaatcg gtctgatgaa aacgaccgaa
     4741 cagctcgaag cgatgaacgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc
     4801 ttttgggcgg gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggtatt
     4861 ttcggcgcgt atttggtcgg tgtaacctgg ctgggcttgg acagcggcat tttctggtcg
     4921 caaatgcaga acaacatcac gatacactac gatgtaatca acggtctgat caagtccgcc
     4981 gcgttcggcg tggcggtaac gctgattgcc gtgcatcagg gcttccattg cgtcccgacc
     5041 tcggaaggca ttttgcgcgc cagcacgcgt acggtggttt cgtccgccct gacgattttg
     5101 gcgatcgact ttgtgttgac cgcgtggatg tttacagatt gacaggccgt ctgaaaacga
     5161 aacaaaaaac atttggatat tcaaggaact ttaatgaaaa agaacatatt ggaattttgg
     5221 gtcggactgt ttgtcctgct cggcgcggcg gcggttgcct ttctcgcttt ccgcgtggcc
     5281 ggcggtgcgg cgttcggcgg ttcggacaaa acttacgccg tttatgccga tttcggcgac
     5341 atcggcggtt tgaaggtcaa tgcccccgtc aaatctgcag gcgtattggt cgggcgcgtc
     5401 ggcgctatcg gacttgaccc gaaatcctat caggcgaggg tgcgcctcga tttggacggc
     5461 aagtatcagt tcagcagcga cgtttccgcg caaatcctga cttcgggact tttgggcgag
     5521 cagtacatcg ggctgcagca gggcggcgac acggaaaacc ttgctgccgg cgacaccatc
     5581 tccgtaacca gttctgcaat ggttctggaa aaccttatcg gcaaattcat gacgagtttt
     5641 gccgagaaaa atgccgacgg cggcaatgcg gaaaaagccg ccgaataaac gtatgccatc
     5701 atattcaaac taggacactt aatcatgaaa aaatcctcct tcatcagcgc attgggcatc
     5761 ggtattttga gcatcggcat ggcatttgcc gcccctgccg acgcggtaaa ccaaatccgt
     5821 caaaacgcca ctcaagtatt gagcatctta aaaagcggtg atgccaacac cacccgccaa
     5881 aaagccgaag cctatgcgat tccctatttc gatttccaac gtatgaccgc attggcggtc
     5941 ggcaaccctt ggcgcaccgc gtccgacgcg caaaaacaag cgttggccaa agaatttcaa
     6001 accctgctga tccgcaccta ttccggcacg atgctgaaat taaaaaacgc caacgtcaac
     6061 gtcaaagaca atcccatcgt caataaaggc ggcaaagaaa tcatcgtccg cgccgaagtc
     6121 ggcgtacccg ggcaaaaacc cgtcaacatg gacttcacca cctaccaaag cggcggtaaa
     6181 taccgtacct acaacgtcgc catcgaaggc gcgagcctgg ttaccgtgta ccgcaaccaa
     6241 ttcggcgaaa tcatcaaagc gaaaggcgtg gacggactga ttgccgagtt gaaggctaaa
     6301 aacggcagca agtaatccat aagccatgcc gccagtctga aggcggcatg gattttttcc
     6361 taccgaacac tatgcataca gaactcaaca acggaacact gcacatcagc ggcgacgtaa
     6421 ccgtcaaaac cctgaccggg gacgcatttg agcgtttccg gcagcaatgc cgtctgaaag
     6481 aaaccattgc cgtcgatttc ggcggtgtca aacgtgccga ttccgcctgc gtgtcgctgc
     6541 tgctcgaagt gctgcgcggc tgcaaaggca gcgtcaggct gaccggcatt cccgaatccg
     6601 tgcgcgcgct gtccgaactg tacgaaatca aagactggct gaaatcatga aaaaaaccgc
     6661 ctatgccttc ctcctgctga ttgggtttgc ttccgcccct gcatttgccg aaacccgccc
     6721 cgccgaccct tatgaaggct acaaccgcgc cgttttcaaa ttcaacgacc aagccgaccg
     6781 ctacattttc gcccctgccg cgcgcggcta ccgcaaagtt gcgccgaaac ccgtccgcgc
     6841 cggcgtgtcc aattttttta acaacctgtg cgacgtggtc agcttcggca gcaatatctt
     6901 gcgcttggac atcaaacgcg caagcgaaga ccttgtccgc gtcggcatca acaccacttt
     6961 cggtttgggc gggcttatcg acatcgccgg cgcgggcggc attcccgaca ataaaaacac
     7021 cttgggcgac acgtttgcct cgtggggatg gaaaaacagc aattatttcg tgttgcccgt
     7081 cttagggccg tccaccgtcc gcgacgcgct cggcacgggt attacctccg tttattcgcc
     7141 caagaatatc gtcttccgca cccctgtcgg acgctggggc acgactgccg tatccgccgt
     7201 cagtacgcgc gaaggcctgc tcgatttgac cgacagtctg gacgaagccg ccatcgacaa
     7261 atacagctac acgcgcgacc tctatatgaa agtccgtgcg cggcagaccg gtgcaacacc
     7321 tgccgaaggt acggaagata acatcgacat cgacgaattg gtcgaaagtg ccgaaaccgg
     7381 cgcggcggaa actgccgttc aagaagattc cgtatccgaa acacaggcag aagcagcagg
     7441 ggaagccgaa acgcaacctg gaacacaacc ttaaagccat gccgtctgaa aatccgtgtt
     7501 cggacggcat tcccttctgt cggaacggac agtccgaaac cacagattgg gctataatcc
     7561 cttttttgcg cataccgcgc gacagcaaag gaaaaaccat gtacgaagtc aaccgcagcg
     7621 tattcgtcct cattccactc gaacccttct ggaactggct gcaaaccctg ccgggcaacc
     7681 accttgacgg actgaccctc gaagacatcc aagccgacgc gaattcctac cttgtccgcc
     7741 cgtgcgaaac cgccgacgaa gtatgggacg aaatcgaaac ccgttttgaa gacattttcg
     7801 ccgccgaact tgccgactgg tgcgaagacg aacgcgagtg gcctgcgctc gatgccgaca
     7861 ttttcaacga atggttcgac atccagcttt ctaccgtcat caccgacctc gaacacgaac
     7921 cgcttgcccg cgaagccttc caacccatca acctgaactg atgaagctga ccgtccgcaa
     7981 ctaccatctc gacggctacg gccatgtcaa caatgcgcgc tacctcgaat ttttcgaaga
     8041 agcgcgctgg gcgttttttg aagaacgcgg actgctgcac gagttggcag gcctcatact
     8101 gattgtcgcc cgcatcgaca tccgatacag ccgccccgcc gtcgaaggcg acgtattgca
     8161 gttttcatgc cgcctgaaaa cccccggcac gcgccgtatt gtgctgaccc agacgattac
     8221 actgccaaac ggcaaaaccg ccgcagaagc cgacatcacc ctgatgcccg tccacgccgc
     8281 cacacaacgc accgtcagcc tgcccgccac ccttgcccgc gcattggaag ccttgtccga
     8341 atgaaaaaaa tactcaccgc cgccgtcgtc gcactgatcg gcatcctcct tgccatcgtc
     8401 ctcatccccg acagcaaaac cgcgcccgcc ttctccctgc ccgacctgca cggaaaaacc
     8461 gtttccaacg ccgacctgca aggcaaagta accctgatta atttttggtt tccctcctgt
     8521 ccgggttgtg tgagcgaaat gcccaaaatc attaaaacgg caaatgacta taaaaacaaa
     8581 aacttccaag tccttgccgt cgcccagccc atcgatccga tagaaagcgt ccgccaatat
     8641 gtcaaagact acggtttgcc gtttaccgtc atgtatgatg cggacaaagc tgtcggacag
     8701 gcgttcggca cacaggttta tccgacttcc gtccttatcg gcaaaaaagg cgaaatcctc
     8761 aaaacttatg tcggcgaacc cgatttcggc aaactctacc aagaaatcga taccgcgctg
     8821 gcacaatagc gatgccgtct gaagcgcaaa aaaacagaaa cgatgttcct gttttttcag
     8881 atcatccgcc ccgacacgtt cagacggcat tcaaccttcc ggcagattct ttttcaccac
     8941 tgccggattg cctgccgcca aagagtaggg cggaatgtct tttgtaacca ccgcgcctgc
     9001 gcccacgacc gaaccgcgtc cgacggttac gcccgccatt acaatcaccc tgtgccccgg
     9061 ccagacatcg tcctccaacg taatcggacg gatttccgtg tagccctcaa agcgtttttt
     9121 gaacggtcaa acttgtggtt atttgaataa aacagacatt ccggctccat catcacattt
     9181 ttgccgacca ccagcccacg gcagatttca cagtttgccc cgatgcccga gccgtcgccc
     9241 aaaaccgtat ccggaaacac atacgccccg cgttcgatat tgaccccgcg tccgatatgc
     9301 ggagaaaccc gccgcgccaa aaaaccgcgc acccgtctgc cgacgatgcc gatgccgcgc
     9361 atatgtgacg gcggcaaccc cgaaccggca aaacgcagca gcaggaaaaa gaattttgat
     9421 cccatcaaac ctccctcaaa agaacttttt atagtggatt aacaaaaatc aggacaaggc
     9481 gacgaagccg cagacagtac agatagtacg gcaaggcgag gcaacgctgt actggttttt
     9541 gttaatccac tatacatttt atcgaatatc ctcttgacga aacaacagaa aatagcgaga
     9601 atgccgagct tgtctgtagc ataggtacat tccctatgcc cgcttttttg aaaggaaatc
     9661 aaatgaaaca aggtattcac ccgaactacc acgaagtcaa cgttacctgc tcttgcggca
     9721 acaaattcgc aaccaaatcc gcgatggaaa aagaaaactt caatatcgag gtttgctccc
     9781 tgtgccaccc gttctatacc ggcacccaaa aaatcgtcga taccaccggc cgcgtggaca
     9841 aattcaacaa caaattcggc aacctgttca aacgctgatt gccgctttgc aagaattaag
     9901 ccctgccatc atttcgatgg cagggctttt taatatttgg cacactgact tctta
//
LOCUS       DAWFIT010000075         9795 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFIT010000075 DAWFIT010000000
VERSION     DAWFIT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.075"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(10..1305)
                     /locus_tag="WGQ85_001675"
     CDS             complement(10..1305)
                     /locus_tag="WGQ85_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0751052.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQSFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WGQ85_001676"
     CDS             complement(1431..2318)
                     /locus_tag="WGQ85_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0751053.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WGQ85_001677"
     CDS             complement(2318..3283)
                     /locus_tag="WGQ85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0751054.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRADPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WGQ85_001678"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WGQ85_001678"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0751055.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4619..4726)
                     /locus_tag="WGQ85_001679"
                     /pseudo
     CDS             complement(4619..4726)
                     /locus_tag="WGQ85_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4805..5074)
                     /gene="rpsO"
                     /locus_tag="WGQ85_001680"
     CDS             complement(4805..5074)
                     /gene="rpsO"
                     /locus_tag="WGQ85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0751056.1"
                     /translation="MALTVEQKAQIVKDFQRQEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5297..6232)
                     /gene="secF"
                     /locus_tag="WGQ85_001681"
     CDS             complement(5297..6232)
                     /gene="secF"
                     /locus_tag="WGQ85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0751057.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVAIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(6236..8089)
                     /gene="secD"
                     /locus_tag="WGQ85_001682"
     CDS             complement(6236..8089)
                     /gene="secD"
                     /locus_tag="WGQ85_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0751058.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8301..8567)
                     /gene="yajC"
                     /locus_tag="WGQ85_001683"
     CDS             complement(8301..8567)
                     /gene="yajC"
                     /locus_tag="WGQ85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0751059.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8695..>9795
                     /locus_tag="WGQ85_001684"
     CDS             8695..>9795
                     /locus_tag="WGQ85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0751060.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRASIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2506 a         2608 c         2431 g         2250 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacgattgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcagc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgtgcata aaaaacattt gcggccaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgcgcagg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc tggaatatcg ggttcaaacc tgcataggta ttgacggcat acaccagatt
      601 tttgcattcg acgctgcctt cgggcgtgta aaccagccaa ccgttttgat gcggttcgat
      661 gcacgtcatc ggggattgct cgaaaatctg cgcaccggct tcggcagcgg cacgagcgat
      721 gcccaaagtg taattgagcg gatgcaggtg tccggataag gggtcgaatt gtgccccttg
      781 gtacatatcg ctgtcaagct gctgtttcaa ctcggcttta tcccaaagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccactgctgc aactcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcgcaa tcaatggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcctgctc
     1021 caagccgacc tgtttttcaa tttcccccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 aggtgtattt tcggacggaa tgatacaggc tgtcgggcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggtcgcca agacattcat ctgcggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgac
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc atgccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca aaatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaac ggcagggtaa
     2581 tcgtcaagaa cgacttgacc ggccccgcgc ccaagtcgga agccgcttcg agcaggcggc
     2641 tgtccagttt caccagctgc gtgtaaagcg gcagaatcat aaacggcaga taggcataaa
     2701 ccatcaccag gtttaacgaa aacgcattgt agaacaaatc taatggctca ctgataatac
     2761 ccatcttaat caataagttg tttacaatgc cgttgtgtcc gagcaaaccc atccacgcat
     2821 aaacgcgcag caggaacgat gtccaaaaag gcagcataat ggcaagcagc aaaccattac
     2881 ggacagaagg gtcggcacgg gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggcg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc acacgctgcc
     3241 ccgggcggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgctgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgaggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagcgtgga ttttccgctg ccggaactgc ccaaaagagc gaaaatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 tttgcagata aggcttggtt gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt cgcaacgtct gatgccgtct gaaactcttg tgaagcgcac gggcagcatg
     4561 aaatggaaca agattccaaa gaactttata ttatattagt ttatgcggtt ttcgggcaat
     4621 atagtggatt aaatttaaac cagtacagcg ttgccttgcc ttgccgtact atttgtactg
     4681 tctgcggctt cgtcgccttg tcctgattta aatttaatcc actataaata tggatttgag
     4741 cttgtcggaa agcaacagaa aagaaaacac cgcccatttt tctgggcggt gtcggaaagc
     4801 gtaattattt acgcagaccc aagcgggtaa tcaacgcgcg atacgtatcg ggctgggtac
     4861 ggcgcaagta ggccagcagg cggcggcgtt ggctgaccat tttcaacagg ccgcgacggc
     4921 tgtggtggtc tttggggttg gctttgaagt ggggggtcag gtcgttgatg cggaaagtca
     4981 acagagcgac ttgtacttcg gaagagccgg tgtcgccttc ttggcgttgg aaatctttaa
     5041 cgatttgtgc tttttgttct acggtcagtg ccataatgaa aactccaaaa atataagaat
     5101 ccccataggg attcagacaa gtttgccaag cctgaagaca acggcaaact ccctatgccc
     5161 aagataggac aacgtggcat tatgacacaa ttcccgcatt tctgcacaat attttaagac
     5221 cgtggaagta accgttaaaa atgccgtctg aagcattgtc tgcttgagac ggcaatgttc
     5281 agacggcata tgcgcttcaa accacgattt cttccttctg tttcgcttcc ttggcgatat
     5341 tgtcgcggct caaaccgaac attagcagaa gcgggctggc aaccaatacg gaagaataaa
     5401 tgccgaacac gatgccgatg gtcaacgcca tagaaaagcc gtgcaaggcc gcaccgccga
     5461 acaccagcat agaaacaacc atcgcctcgg tcgaaccgtg ggtaatgatg gtgcggctca
     5521 tcgttgcggt aatcgcgttg tcgatgactt ccggcacggc atgtccgcgc atcgccggct
     5581 tgcggaagtt ttcacggata cggtcgaaga cgacgacgga ttcgttcaca gaatagccca
     5641 atacggcaag gatacccgcc aagacggtca gtgaaaattc ccattggaag aaggcaaagc
     5701 agccgagaat aatcacgatg tcgtgcatat tggcgataat ggcagatacg gcaaaacgcc
     5761 attcgaaacg catcgacagg taaataatga tgccgatagc gacaaaacct aaagccatca
     5821 atccattact taccaattcc tcaccgactt gcgggccgat aaattcgact tggcgcaagg
     5881 taacgtcggg actgtctttt ttcagcaaat ccataacctg attggacaac tgtgcggaag
     5941 taacaccttc tttgttcggc aggcggatca tgatgtgttt gttcgtaccc aatgcctgaa
     6001 cctgtacatc acctattttc agcgtatcga ggcgttcgcg catcttattg acatccgcac
     6061 cctgctgata ttggacttcc attaccgtac cgccggtaaa ttcgacagag aaattcagac
     6121 ctctggtaac caaaaagaat acggcggcaa taaaggtaac caacgaaata aaggtcgtca
     6181 gtttgccgta gctcataaac ggaatatcgc gtttgatttt aaagagttcc atagcttact
     6241 ccttgcctcc tgccatttcg gctttcggct tccacaccga accaatggaa atattctgca
     6301 atttgcgtct gcgtccgtac cacagattga ccaacgcacg gaatacgacg acggacgaat
     6361 acatcgaagt cagaataccc aaacagtgta cgaccgcaaa accgcgtacc gggccggaac
     6421 cgaataccaa aagcgcgata ccggcaatca gcgaagtcag gttggaatcg acaatggtcg
     6481 cccatgcgtg ttggaaaccg agattgattg cctgctgcgg cggcacgccg gcacgcaatt
     6541 cttcgcggat acgttcgtta atcaagacgt tggagtcgat tgccataccc aaagtcaacg
     6601 ccagcgcggc catacccggt aacgtcaacg ttgcctgcat ggcagacaaa ataccgatta
     6661 ggaacagtat gttggcactc aatgcaatgg tagaaaagaa acccatcaga cgatagtaaa
     6721 ccaccatgaa tgcagcaacg atggcaaaac cccataaagt cgaatggaag cctttttcga
     6781 tgttctcctt acccaaagac ggaccgatgg tacgttcttc gacaatctgc atcggcgcgg
     6841 caagagaacc ggcacgcaac agcaaagacg tatcattggc ttcggctgtc gtcatgcttc
     6901 cggaaatttc cacgcgtccg ccggtaatgg cagtacggat aaccggcgcg gttacaacct
     6961 cggattttcc ttggtcgatc aaaaccatcg ccatgcgttt gccgacattt gcggcagtca
     7021 gttcgccaaa aatgctgcca cccgcgctgt ccaagctcag actgacggca ggtgcgccca
     7081 tttggtcgaa actcggttgc gcatcgttga tgttgtcgcc cgtcagctcg acctgtttgc
     7141 tgattagcag agtttcggga cgatctccgc cgcttgaaag cagctcataa ccgctcggca
     7201 cgttgccttc caatgcctcg cgcaacttgg caggatcgtc ctccaccata cgcaattcca
     7261 aagtcgcggt acggccgatg atgtcttttg ccttggcagt atcctgaacg cccggaagct
     7321 gcacgacgat acggtcggca ccggactgct ggatgacggg ttcggccacg cccaactcgt
     7381 tcacacggtt gtgcagggta gtgatgttct gtttgaccgc atcggaacgc actttattga
     7441 ccgcctcttc cgaaagcgtc aagacgatat tgctgccgtc tgaattcagc gttgcttcag
     7501 gaaacagctt gcgcaactgc ggcagagcct tttgcacatc acctgcatcc tgcaaaggga
     7561 cggtcaggct gtttccagcc tgacgcaccg tgccgctgcg gattttttcg cggcgcagtt
     7621 cgcggcggat gtcgcccgaa taacgttcaa acgttttctg catcgccgct ttcatatcga
     7681 cctgcatggt gaaatgcacg ccgccgcgca agtccaaacc caaaaacatc ggattggctt
     7741 tgattttcgc catccattcg gggctgtccg ccaacaggtt gagcgcggta atataccctt
     7801 cgcccaaagt gttttcgatg acgtcgcgcg ctttaagctg cgtttctgtg tctttgaaac
     7861 gcactttcag tgaattgtcc acaacaaaca tcccgtcggt ctgaatacct gcgtttttca
     7921 gcgcggcatc cactttgaat tgagtctgtt cgttgatgat gatggcttgt cggttggtcg
     7981 atacctgcac ggcgggtgtt tcgccgaata ggttgggcag cgaatacact gcggcaaccg
     8041 caatcgtgaa cacaatcagc agatatttcc ataaaggata acggttcatc attgttcctt
     8101 aatggttgga accccaccct ttcggtggtg tcggaatcgg gctatttcag aagaggcaaa
     8161 aacccttccc agccaggcaa gaccggaaag cggcatcctg aatatgccgc cctgcgtgtc
     8221 ggaacatggt caagccttcg gttggaattc aaaacaaagt gccgcattcg ggcttttccg
     8281 atgcggcttg tcggcacaaa tcaatcgact tttgcagaaa tcgcattgcg ttccacttcg
     8341 acctcgattt ttgtaccctg tccgatatcc acggtaaaaa actgttcgcc gactctggtt
     8401 accttaccct tgaaacctgc cgccaagacc actttgtcgc cgactttcaa ggcggcaagc
     8461 attgcctgat gcgctttgaa tttcttttgc tgcggacgca tgatcaggaa gtagaacacc
     8521 accataatca acactaaagg agcaaattgt gcaacagctt gattcataat ttatccgttc
     8581 tttctaatat ggttgaaaat cgagaggggt atataataac ataagaccgt aaacaatata
     8641 tcgggtttgc cgtccgtacc gaccgtatac cgcagcctgc ccgtccacaa acccatgtcc
     8701 ttgacccgcc taatcttgaa attctatgca ctattgcgcc tttttttggg caaaaacgcc
     8761 cgcaccgcat ggatttcgca tcccgcctgt gccgggcacg aacccggcgc aaaccatccc
     8821 gattcgcccg accgcatcct ctgcatcgag caggcattgc gccgcgccag tatttggcag
     8881 cacctccaaa ccatagaggc ggaagaaatc agcgatacgc gcctcgcact tgtccactcg
     8941 agcaaatatc tgaaccgttt ggaatcttgc ctgccccaaa aaggcaagat ttcccgcctg
     9001 gataacgaca ctgcaatcag cacaggctcg ctgtctgccg cacgctttgc cgccggttcg
     9061 gcagttcagg cagtcgacat ggtcatgaac cgtaaagcat ggcatgcctt ttgcgccgcc
     9121 cgcccgcccg gacaccatgc gggcagcggc aaagccggcg gattctgcct gctgaacaac
     9181 gttgccgccg gcgtcatgca tgccattgcc gaataccgcc tgaaacgcat tgccgtcatc
     9241 gatttcgatg tccactacgg cgacggtacg gcagaaatat tcaaagacga tccgcgcatc
     9301 ctgtttttca acctgtttga aaccgacctt tttcctttcc ccgaaaacaa cgatatgccc
     9361 gacggcggca atatggtgca cctgcccttg ccgccgggaa cgggcagccg cacattccgc
     9421 gaagccgtcc gcagacagtg gctgccccgg cttgccgcat tcaaacccga actggtgctg
     9481 ctgtcggcag gattcgacgc acaccgtcta gacgaatcgg gcaggctcaa cctgcacgag
     9541 gcggattttg cctggctgac acacaaaatc attcagacgg catcgggctg ccccggcaaa
     9601 atcgtatccg tactcgaagg cggctacacc cttgaacctc tagcacaatc tgctgccgag
     9661 catatccgcg tgctggcagg gctgggcaaa tccgatgccg caaccgccta tcagaaaaca
     9721 ctggacccaa caaaaaaacg gtttgccaaa ccgaaaaccg gacaggtgcg ccaacctacc
     9781 caatccgacc gttac
//
LOCUS       DAWFIT010000076         9341 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFIT010000076 DAWFIT010000000
VERSION     DAWFIT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.076"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..354
                     /gene="rfbC"
                     /locus_tag="WGQ85_001685"
     CDS             <1..354
                     /gene="rfbC"
                     /locus_tag="WGQ85_001685"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0751061.1"
                     /translation="QTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLSAQNQ
                     CQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIDWPLQTAPLLS
                     PKDLAGKVLAEAVTF"
     gene            378..2492
                     /locus_tag="WGQ85_001686"
     CDS             378..2492
                     /locus_tag="WGQ85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0751062.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2629..3888
                     /locus_tag="WGQ85_001687"
     CDS             2629..3888
                     /locus_tag="WGQ85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ0751063.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            4256..5662
                     /locus_tag="WGQ85_001688"
     CDS             4256..5662
                     /locus_tag="WGQ85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0751064.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5880..7094
                     /gene="gltS"
                     /locus_tag="WGQ85_001689"
     CDS             5880..7094
                     /gene="gltS"
                     /locus_tag="WGQ85_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0751065.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            7193..8209
                     /locus_tag="WGQ85_001690"
     CDS             7193..8209
                     /locus_tag="WGQ85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0751066.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8220..8417
                     /locus_tag="WGQ85_001691"
     CDS             8220..8417
                     /locus_tag="WGQ85_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751067.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8435..8584
                     /locus_tag="WGQ85_001692"
     CDS             8435..8584
                     /locus_tag="WGQ85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751068.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
     gene            8940..9233
                     /locus_tag="WGQ85_001693"
     CDS             8940..9233
                     /locus_tag="WGQ85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751069.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT         2384 a         2695 c         2192 g         2070 t
ORIGIN      
        1 caaaccgaaa acacacaagg caaactcgta cgcgtagttg tcggcgaagt gttcgacgtg
       61 gccgtcgata tgcgcgaagg ttcgccgact ttcggcaaat gggcgggcgc aaccctgtcc
      121 gcgcaaaacc aatgccagct ttggataccc gaaggcttcg cgcacgggtt ttgcgttttg
      181 ggcgacgcgg cggaagtcgt gtacaaatgc accgactatt acaagcctga agccgaacag
      241 gttttaatat ggaacgaccc gacagtcggc atcgactggc cgcttcaaac cgcgccgctg
      301 ctgtcgccta aagacttggc aggcaaagta ttggccgaag cagtcacgtt ttaagactat
      361 tcaggccgtc tgaaagaatg ttcctctttt cagacggcct acaatccatt aataacaata
      421 atcgacgaaa acgcattgtg aaaaacgcct acatcccctc tcgcggcatc cgcaaaatcc
      481 cctatctctc cactctattg cctgagtttc atatctgcaa ggatgggaaa ggagcagagg
      541 ctgttatcgg ctggggtttg cgcccgacga cacacaaagc gcgtgctttt gccaccgaac
      601 accagcttcc ctttattgct ttggaagacg gctttttacg ctcgctcgga ctgggcgtct
      661 ccggttatcc cccctactcc atcgtctatg acgacatcgg catctactac gacactaccc
      721 gcccttctcg tttggaacaa ctgattcttg ccgccgatac catgccgtct gaaaccttgg
      781 ctcaggcgca acagacgatg gatttcatcc tgcaacacca cctgtccaaa tacaaccacg
      841 cgcccgaact ttcagacgat catcctttac gctccccatc caagcctgaa actgtcctca
      901 ttatcgacca aaccttcggc gatatggcca tccaatatgg cggcgcagac gcctctacgt
      961 ttgaactgat gtttcagacg gccttaaatg aaaacccgca agccgatctt tgggtcaaaa
     1021 cccaccccga tgttttgtgc ggcaaaaaac aaggctatct gacccagctg gcgcagcaac
     1081 accgcgtcca tcttttggct gaagacatta atccgatttc gctgctgcaa gcggtggaca
     1141 aagtgtattg cgtgacctcg caaatgggtt ttgaagcctt gttgtgcggc aagcctttgg
     1201 taacgtttgg tttgccgtgg tatgccggct ggggtgtaag cgacgaccgc catcctgaaa
     1261 tcaaccgcct tgttcaaacc caacgccgcg ccacccgcaa cttgctgcag ctcttcgccg
     1321 cagcctatct gcaatacagc cgctacctca accccaatac cggcgaagca ggcagcctct
     1381 ttgatgtcat cgactatctg gcgacggtca aacgtaaaaa cgacaaattg cgtggcgagt
     1441 tatattgcgt cggtatgtct ttgtggaaac gcgcggttgc caaaccgttc tttaacgtac
     1501 cctcttgccg tctgaaattt atctcttcca cccaaaaact ggcaagggtc aaactgtccg
     1561 acgatgcacg catcctggct tggggcaacg gcaaagaggc catcgtccgc tttgccgaac
     1621 aacaccacat ccccctgctg cgcatggaag acggctttat ccgctcggtc ggactcggct
     1681 ccaacttagt gccgccgctg tcgctcgtta ccgacgatat gagcatttat ttcaatgccg
     1741 aaaccccgtc ccgtcttgaa tacatcctac aaaaccaaaa cttcgacgat caagactttc
     1801 agacggcctt gaagctgcaa aaaatgctga ccgaaaacca catcagtaaa tacaacgtcg
     1861 gcagctcaga cttcaccgcc ccgtcaaccg acaaaaccgt gatcctcgtt cccggccagg
     1921 ttgaagatga tgcgtctatc cgctacggtt cgccccaaat ctaccgcaat ctggatttgc
     1981 tccgtaccgt acgcgaacga aaccccaatg cctatatcat ctacaaaccg catcccgatg
     2041 tagtcagcgg taaccgcatc ggccatattt cccctgaaga tgctgcacga tatgccgacc
     2101 aaaccgccga gcaaaccgac atcctgacct gtctccaata cgcagacgaa gtacatacca
     2161 tgacttcgct gaccggtttt gaagccttgt tgcgcggcaa aaaagtcagc tgctacggcc
     2221 tgccttttta cgcaggctgg gggcttaccc aagatctgct ccccatcccg cgccgtagcc
     2281 gcagacttga gctttggcag ctgattgccg gcacgctcat ccactatccc gactacatcc
     2341 accccgaaac ccatcaggcc ataaatgcag aaaccgcagc ccaaatcctg atacgacaaa
     2401 aaaatatgca aaaaaacaac aacggattac atcgcgggta ctttgccaaa aaattaggta
     2461 aaatcaaaca actatatcga tctttcaaat aaataccatc aaagttaacg atgcgtcata
     2521 aacttgcctc tattgcggca tcattgcctt tgcatcgtta attctcttgg cgtatgcttg
     2581 aaagttcaac ctaaaactat tacataaaaa acaaaaccac attgcaacat gaaacagacc
     2641 gtcctcaaaa ataacctgca aaacctgctt gaaagcgcag aaaatatcct gctgcttcaa
     2701 ggccctgtcg gcgatttttt tctgcgcctt gccgactggc tgactgcaaa cggcaaaacc
     2761 gtacataaat tcaactttaa tgcaggcgac gactattttt atccgcccac tcaagcgcat
     2821 accgttgttt ttaacgacaa ctacgatgcc tttcctgagt ttttgcaaga atacatcact
     2881 caacatcaca tccaggccgt tgtctgcttt ggcgacacac gcccttatca cgtcattgca
     2941 aaacgcattg caaacgaaaa ccaagccagt ttctgggcgt ttgaagaagg ctatttccgc
     3001 ccctactaca tcaccttaga aaaagacggc gtcaacgcat tttccccgtt gccgcgccgt
     3061 gccgactttt ttcttgaaca attccctaag cttgcccagc aagaatataa agcgccaacg
     3121 ccggtacacg gcggttttac gcccatggca aaaaacgcta tccgttacta tatcgagttg
     3181 ttccgcaatc cacgcaaata ccccgactac atccaccacc gcgcacccaa tgccggccat
     3241 tacctcaaac cgtggtcgct ctccatcctc aagcgtttga actactatat tgaagacatc
     3301 caaatcgcca aacgtgtgga agcaggcaaa tacggcaagt tttttattgt tcccttacag
     3361 gtattcaacg acagccaagt ccgtatccat tgcgactttc ccagtgtccg cagcttcctg
     3421 ctccatgttt tgagttcatt tgccgaacac gcgcctgccg ataccaacat catcatcaag
     3481 catcatccga tggaccgcgg ttttatcgac tactggcgcg acattaaacg ctttatcaaa
     3541 gaacaccccg aactcaaagg ccgcgtgatt tatgttcatg atgtccccct gcccgttttc
     3601 ctgcgccacg gtctcggcat ggtcaccatc aacagcacca gcggcctgtc cggcctgatt
     3661 cacaatatgc cagttaaggt tctcggccgt gcctattatg atattcccgg cattactgac
     3721 caaaatacct tggcagaatt ttggaatcat ccgacaccgc ctgacaaaga gctgtttcat
     3781 gcctaccgta tgtaccacct caacgtgacc caaattaacg gcaacttcta cagtcaggtg
     3841 tttttcccca acaaaaaaac ctccaactct tccacaccag taatctgact tagcgaagga
     3901 aagttcaggc cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg
     3961 ccatgtaaat aaaacacaaa aacctgcatt tatcattaac aataaattac aaaaacagta
     4021 taatgaccga gctgtcatga gcgcataccg actcaacctg agccctttgt aacacacaaa
     4081 atatggatgt atccctaggc aaaacaatat aacaagccaa acatcctaaa gataagccgg
     4141 caaggcaaca cactctacaa aaccgtgcca agcaatattt ttacaaagcc ctcaaccggt
     4201 atcgccgccc atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac
     4261 cgccgctcct gatatcctct accggcaagc cgccgccctt ttggaacaat ccaataccgc
     4321 ccaagccctg cccctgctgc aacaggccgc agagcaggga tatacggaag ctgcttttgt
     4381 attgggcaac catctgctga aaaacggtca tccggagcag gcacttccat ggttggaagc
     4441 cgccgcggcc caacgccatc ccaaggcact cttcaccctg ctgcaacaac gcgaacacaa
     4501 cggcaccccg accggacagc ttcttaacga atacgcctgg ctgagtgagc aaggtcacct
     4561 cgaagcccaa ttaattctca tgcgttacca cacgcagcgc aacgatccac aatcgctcta
     4621 ctgggcggag cttgccgctg cccaatatgc cgcacctgcg tattaccatt tggcacgcca
     4681 tcatcaacgc caaggcgacg ttgcgacagc catcgaacaa tacgaaaaag cggcagcact
     4741 cggcataact gctgcctgct ggcaactcgg tcaaatctac ttctacggta caggtgtcag
     4801 ccccaaccac gcacaagccg aacactatct cgaacaagcg gcgcaagccg gccacatcgc
     4861 cgcacaaacc ctgctggccg accttcttgc cgcccaacgc aaacccgaag ccttagaatg
     4921 gtatcgtcgt gccgccaata aggagcaagc ggaagcacaa gccaagctgg cccaatacgc
     4981 cctgaccggc gaactttccg aacgcgatcc gttccaagcg gcgcgctatg ccaaagccgc
     5041 tgccgagaaa gaccatcctg aagccctgaa aatcatgggc gacctctacc gctacggcct
     5101 cggcatcaaa gctgacaacc atatcgcgca cgattactac caccgtgctg ccgcgctggg
     5161 ttctgccgcc gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata
     5221 cgaacaaatc aaaactgccg ccctgcaaca acaacaaacc gaaaccatct accgtttggc
     5281 ggaagcacaa gcctgcgcca tcggccgtcc cgccgactac aatgccgcgc gaaaaaatta
     5341 catggaagct gccgggttcc accataaaaa cgcagcggca gccttaggcc gcatctacca
     5401 ttacggcctc ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccattgctgc
     5461 cgaacaaaac cacccttccg cccaatacca cctcgcctgt ttttactatc acgggcaagg
     5521 tgtcggctgt catgttccga ccgcctgcta ctggctgcag gccgccatcg gcaacggcca
     5581 cacttcggcc gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc
     5641 catcggacaa aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta
     5701 ttcccctaat atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga
     5761 aaacaccttg tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac
     5821 acactacatc cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca
     5881 tggaatggga attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg
     5941 gtaaatttct ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtag
     6001 ccggcggttt gattgccgct atcgtcctgt tcgccctgca cgaggcgtac ggcgtgagct
     6061 tcaaatttga gaaaccgctg caaaatgcgt ttatgctgat tttcttcacg tccatcggct
     6121 tgagcgcgga tttttcccgt ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga
     6181 ttgtgggcgg atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg
     6241 gtttggatcc gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta
     6301 cgtcaggtgc gtggggacct aattttgaaa cgcaatacgg cttggtcggc gcaaccggtt
     6361 tgggtattgc atcggctact ttcgggctgg tgttcggcgg cctgatcggc gggccggttg
     6421 cgcgccgcct gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg
     6481 acgacaacgc ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat
     6541 ctgccgttga aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg
     6601 gcttcgacaa agaatatctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg
     6661 tggtcatccg caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg
     6721 atgtgttcgg caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac
     6781 tgtgggagct gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg
     6841 tgatggtttt gtacgcgact tttgttacct atgtctttat ggggcgcgac tatgatgcgg
     6901 cagtattggc tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa
     6961 atatgcagtc cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta
     7021 tggtcggcgc gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt
     7081 tctttaaagg ctgattttcc gcctttccga caaagcacct gcaaggttta ccgcctgcag
     7141 gtgcttttgc tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg
     7201 gaaactcatt gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc
     7261 caaggcattg gaatgcgcca accccgccgt gttgcaaggc atacgcggca atattcagga
     7321 aacgctcacg caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc
     7381 cgacaaaatt atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac
     7441 gcaggaaggc gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac
     7501 gcttgccgat gccaaggcaa acagccccct gctgtacggg gaaaccgctt tgtcggatat
     7561 tgtgcggcag aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt
     7621 ccgcttcctg cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc
     7681 ggcgcaaacg ctgtctgtcg cgttgctgcc ttacggcgtg aagagcatcg tgatgataga
     7741 cggcaaggcg gtaaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga
     7801 agaaccgtcc aaacccacgc ccgaagacat tttggaacat aatgccgccg gaggggatgc
     7861 agacgtaccc caagccgcag aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg
     7921 cgagcgtgcc gataccgtta ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa
     7981 ccagcgtgcg gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa
     8041 agagttggtc ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc
     8101 cgccgcgcag gcagaccggc aggaatacgc cgaatacctc aagctgcaat gcgacacgcg
     8161 gatgacgcgc gaacggatac agtatcttcg cggctattcc atcgattagg ggcaaaccga
     8221 tgaataccgt cccaaaaagc aggattcccg tcaaaccgct gcccgaaaaa accacagacg
     8281 aagccaaagt cgaaaaatgg cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg
     8341 cagttgccgt cagattgggc gaaaacggtt ttaccgaaga acagatggaa aatatcgcca
     8401 acctgttcgg cagataaaga gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt
     8461 ttcagacggc attttgacca atacggtacg caggcgcaaa acagccggct tttcctgtgt
     8521 tgcctatgct gatgtttcaa cacacaggac gatacaaaaa acgtcgccct atgtgccgtc
     8581 ctgattcgga agggttacgc tccttccaaa tatagtggat taacaaaaac cggtacggcg
     8641 ttgtctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     8701 gttccgtact atctgtactg tctgcggctt catcgccttg tcctgatttt tgttaatcca
     8761 ctatattgtc cattcggcag ggaaagcctg aagaagaatt ttcccgcaag atggccggct
     8821 gcctaaccga cttggaaaac tgtttggaaa aatacctcga acagttcggg ccggtatcgg
     8881 aaagtatcga agcctgtacc gtcaaactgc aggagtaacc gtcgttcttc aacaggctga
     8941 tgaaaaccaa cgacaagctc aataggcaga ttgacgtgtt gcagaaacag tcggcagcca
     9001 tccacaacga agcctatatc gaaatgaaca cgctgcttta ccgccaccgc gaagtggttt
     9061 ccgtccacaa ccgcaaagca gattatgcgg aaaagggcaa agagcagatt gcgctgttcc
     9121 cgcgcggatt gaacggcatc accaagctgc ccgccgccgt cctgttgccc gagcgtccct
     9181 accattttga tatgaaggag gttctgcata tattctcctg gataccgagg taggcgtatc
     9241 ggctgccgga taaaaatgca ccccgtcatt cctgtctgta cgggggtagc ggcagtgttt
     9301 tcctgcttcg ttttatagtg gattaacaaa aaccagtacg g
//
LOCUS       DAWFIT010000077         9290 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFIT010000077 DAWFIT010000000
VERSION     DAWFIT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.077"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(49..3093)
                     /locus_tag="WGQ85_001694"
     CDS             complement(49..3093)
                     /locus_tag="WGQ85_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0751070.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAGFEQAQHSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSNGQYLSETEAFGHDSRAVCPFEDVPSERPSCRASDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3258..3938
                     /locus_tag="WGQ85_001695"
     CDS             3258..3938
                     /locus_tag="WGQ85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0751071.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3967..4326
                     /locus_tag="WGQ85_001696"
     CDS             3967..4326
                     /locus_tag="WGQ85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0751072.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4992..5528)
                     /locus_tag="WGQ85_001697"
     CDS             complement(4992..5528)
                     /locus_tag="WGQ85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0751073.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5562..5918)
                     /gene="folB"
                     /locus_tag="WGQ85_001698"
     CDS             complement(5562..5918)
                     /gene="folB"
                     /locus_tag="WGQ85_001698"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0751074.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5974..6576
                     /gene="plsY"
                     /locus_tag="WGQ85_001699"
     CDS             5974..6576
                     /gene="plsY"
                     /locus_tag="WGQ85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0751075.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPTTALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            6664..7509
                     /locus_tag="WGQ85_001700"
     CDS             6664..7509
                     /locus_tag="WGQ85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0751076.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            7755..8729
                     /locus_tag="WGQ85_001701"
     CDS             7755..8729
                     /locus_tag="WGQ85_001701"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0751077.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGCVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(8888..9106)
                     /locus_tag="WGQ85_001702"
     CDS             complement(8888..9106)
                     /locus_tag="WGQ85_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0751078.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2154 a         2723 c         2171 g         2242 t
ORIGIN      
        1 aaatgccgtc tgaaacagtc ttcagacggc atttccattt gcaaaaaatc aagcattgtc
       61 caaggggacg agaatcgtac ggttgccgtt gtgttccggt gcggacacaa tgccttccgc
      121 ctccatctgg tcaatcagac gcgcggcgcg gttgtagccg atacgcaagg cgcgctgtac
      181 gcccgaaatg ctggctttgc gcgttttcag gacaacggat acggcctcgt cgtacatcgg
      241 atcggtttcg tcgtcgccgc tgcgcccgat gccgggcagc tcttcgctgc cgccgccgct
      301 caaaatatcg tcaacatagt ccggttcgcc aaactgcttc agatattcga ccacgcggtg
      361 cacctcttca tccgaggcaa acgcgccgtg aacgcgctgc ggataggccg tacccggcgg
      421 caggaacagc atatcgccct gaccgagcag gttttccgcg cccatttggt cgagaatcgt
      481 gcggctgtcg attttgctgg atacttgaaa cgcgatacgc gtcgggatgt ttgccttaat
      541 cagacccgtg atgacatcga cgctggggcg ttgcgtggca aggataagat ggatgcctgc
      601 cgcgcgggct ttttgggcga ggcgggcaat cagttcctcg attttcttgc ctgccgtcat
      661 catcaaatcg gcaaactcat cgaccacgac cacgataaac ggcagttttt ccaaaggttc
      721 gggatcgtcg ggcgtgaggc tgaacggatt gccgattttt tctccccttg ctgcggcttc
      781 ggcgattttt tgattgaagc ccgcaagatt acgcacgccc ataaagctca tcaggcggta
      841 gcgtttttcc atttcgttaa cacaccagtt cagcgcgttt gccgccagct tcatatcggt
      901 aacgacaggg gcgagcaggt gcgggatgcc ttcgtaaatg ctcaattcca gcattttcgg
      961 atcgatcata atcatacgca cgtcttccgg cgcggctttg aaaagcatag acagaatcat
     1021 cgcgttgaca cccaccgatt tgcccgaacc ggtcgtgccg gcaaccagca aatgcggtgc
     1081 tttgcccaag tcggttacaa cgggctgtcc ggtgatgtcc tgaccgagcg cgagcgtcag
     1141 cttggatttg gattcggcaa actcgggcga attgaagatt tcgctcaggc gtatcatttg
     1201 gcgtttcggg ttcggaagtt ccaaacccat acaggttttg ccggggatgg tttcgacaac
     1261 gcggatggaa gccacgccga gcgaacgcgc caaatctttt tccagattca gaacggaatt
     1321 gccgcgcacg ccgacatcgg gttcgatttc ataacgcgta attacggggc cggaataaga
     1381 atcgacaacc ttgaccttga ctttgaactc cgccaatttt tcttcgatgg tgatgctgtt
     1441 ttccagcagt tcttcttcgg tttgcgtcgc ctcgggattg aacagcggag gcagaagcag
     1501 gtcggttgtc ggcaggtgtt cggataccgc accggtttct tccgatggga acgccccttc
     1561 atccgcttcc gtatccgatg cccggcagga cgggcgttca gacggcacat cttcaaacgg
     1621 acaaaccgcc cgactgtcat gcccgaacgc ttcggtttcc gacagatatt gcccgtttga
     1681 ccgctctgcc gcaccttcac tgccgtcatc tgcaatagcg gcggtttcct cctgccaacc
     1741 tccattcaaa acatcatcgg caagatggtc ggtttcggca atgcggctgt gttgcgcctg
     1801 ctcgaatcct gccggcggtt cataggtacg gttgtagatt tccgatacgg gaggcggcgg
     1861 cggaatatcg attgcgggca tgggaacttt cggcacttcg ggtggttcga ccacccaagc
     1921 atcgggcgcg gcaggttctt caatatggat atcggcagtt tcatcataaa ccggaccgcc
     1981 gtatcccaca gacgaaacag tttccgtgaa aacattttcg gtttcagacg gcatttcgac
     2041 atcccgtttc ccgacaaccg tccgactttc cggaatcagg cgcgaaatcc gcgcctccgc
     2101 agaaaccgtt gccttgttcg gttctttcaa attgacggaa acgcggcggc gggcatcgcg
     2161 gacggctgtg ccgtctgaaa cggactgccc ttgggaaaca tccggggatt ttgcctccgc
     2221 ctgccctttc ccctgccctg catgacggcg gaacggcgca ttccgtgcat tttcggcttt
     2281 gactgcagaa aaggcagacg gatgctgctt ctcaaaatac ggctcaaatc cgtaatccgc
     2341 agactcggaa aacgcttctt tgtccgcatc gaacatacgg gaatacgtgc gttgaagcac
     2401 agggtcgtcc aaaccgataa tctgcagccc ttccatcggg gtatcggaca cgcggacttt
     2461 ggggaccgct gtttcgtttt tctcaaatgc atcgatatag cgttttttcg tttcccttaa
     2521 agccgcgctg tttaaagcac gcgttgcttc ttccaaagtg atttccttaa aaaccggacg
     2581 gacgggcgaa atttcgcttt cagacggcat caacccttca gcaatactcc ggtcgaatgg
     2641 gatgcggcgg ttgtcgatta cggcagttgc aatgtcttcc gtatcggcag cctcttcctc
     2701 cgcagcttct gcttcttccg tttccgcttc ttccgtcccg tttccactgt ctgaatatcc
     2761 gtcggatgcg gtcggaacat ccgcacgatt tgcagaagca ggcggaacgg gcctgtccgg
     2821 ttcggattcg tgcgccagat agtttcggca gaaacggacg acaccgacaa acagccaata
     2881 cactgccgtt ttgacggcat ggaaaagcat cagggcaagt tcgggaaaat cgggcatacc
     2941 gtctttgatt tcaggcagct ccgcctgttt ttcccctttc ttttcctgcc acgcagaaac
     3001 ctcgcgcatc cactcgcgtt cggattgtgc gcggacaaaa aacaagccgg caagcgcaag
     3061 caacaaaata acgatcaaaa ctatccaaaa catatgccgt ttcccgcaag acaaacgatt
     3121 aaacgggttt attttaacgt gtttccatcc caaaaaaaac aaccggtcgg caattctgcc
     3181 gaaaccgatt cggctttcgg tttgcccccg ccgacagtat aatagccgtc ccttccctta
     3241 atctttgcgg cattgccatg attttccaaa cagtctggtt ttcagatatg gtactgtcgg
     3301 tttcgtggat tgtccttatc ctgatactgg cagcttccgc gccgtccgca ttccgctcgc
     3361 ttgcccgcta ccgttccgcc cttccactgt gtaccgtaat tttttccgca gcatggtgtc
     3421 tgaacgcctc tgccggcggc ggacagcttg cacagatgaa ctaccacctg ctcgccgtca
     3481 atctggttgc actgatggtg ggcacttccg ccgccctttg gcttgccgcg ctgctgatgc
     3541 tgccctactg cctgctgttt gccgattctg ccggcgcata tccgccgaac gctttggtgc
     3601 tgattctgcc cgcgctggtc gtcaaccgcc tgtcgcgtat gctggtcaac cgcctgccgc
     3661 ccaatatttt catctttatt ttcgtcaacg gctttctcgc ttccgccgcc ggcattctgt
     3721 tgaccgggct ggtgctgacc ggtattttgg atgctgcaaa cgcttttccg tccgaaatat
     3781 tgtggacgac cgccctgccc gtctttattc tgctggcgtg ggcggaagcc ttcctcagcg
     3841 gcatttcaac tgccatattt gttgcactga agccccattg gatcaacact ttcgacgaca
     3901 accgctatct gaagtccgac cgcggcatat ggcggtagcc ttcagacggc ataggcagac
     3961 aaaaccatgc tttccaacat catccccctt tccatcggtg cggcactcgg tgcaacggcg
     4021 cgttggctgc tcaacctcgc cgttcctgcc gccatgtctc ccgccacggg caacctgttt
     4081 gccaactgga cgggcgcgct gctcatcggc atctttgccg aaaccatcaa tcacccgcaa
     4141 tggaagctgc tgctgattac cggttttctc ggcagcctga ccacgctttc cggattttca
     4201 ctggaaaccg taaccctgct ccaatcgaat cgtcctgctt cagcacttgc caatattttc
     4261 ctacacacgg caggttcgct gctgctgact tggctcgggc tgaagatagg ggcggcagtc
     4321 aaataaccgc aacaaattgc ctccctttgt tttgacataa tctataaatt agcttcgctt
     4381 gtttaaatac tggctgtgcc aattatagtg gattaacaaa aatcaggaca aggcgacgaa
     4441 gccgcagaca gtacagatag tacggaaccg attcacttgg tgcttcagca ccttagagaa
     4501 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactatacc
     4561 gttctcaaat tcaaattggg cttttaggtc tataacaaag aaaaagcctt ggtaggataa
     4621 acctaccaag gctttttctt aaaaaaacgc tctattaccg tgcctaaaaa tgcaaaaaag
     4681 taaccgtcat aacccttttg ccaaaatcaa tatcatcatc cgatgaatga attgacggta
     4741 tataaggacg gtatataaga aagaattaat aagctcataa accggtaaga aaaatcagta
     4801 acaaagatat tcattattaa ccattttaaa aattatcatg ttcgtttggg taattattat
     4861 acccttttcc ctcttattac taaagcacga atgactttaa aggacgcgcc cagagacaag
     4921 ccgaaaaggc atatctcatc cgccaaacaa aatgcccacc gacttactcc ggcaggcagt
     4981 tcaacaccct gtcaatcctt catcagccag tattgcaaac cgattaatgt cttgccgtct
     5041 ttaatttcat cgtttgccaa tgcctgacgg acttcttctt tcgacatcaa tacggtttcc
     5101 gtaatctcgt cttcgtcatt ggcaagcgta ctgcccaaac gcacgccttc cgcctcgaac
     5161 agatacattt tttcgttgca aaaaccgacc gccgtataaa aactgtacag caggcgcacg
     5221 ctgtcggcaa cataaggtgt ttcctccgcc aattctcgca gcgcacacgc tgccatatcc
     5281 tcgcctgcca catccagctt gcccgcagga agttccaagg tcgcctgatt tgccgcataa
     5341 cgccactgcc gcaccaaaac gaccttttca tcatccgtta ccgccaacac gcacgccgcg
     5401 cccggatgac ggatgacgat acgctgccct tcattgccgt tgggcaacct gaccttatcc
     5461 ctgctgatgc tgacaaaacc gccctcgtaa atggtttcgc cgcctaattt tacttccctc
     5521 aaatccactt catatctcct ttccgtttcc tattctgccg ttcaatcttc acgcttgccg
     5581 cgctcgattt ccacgccaac ctcgcgcacg ccttccaaaa tacccggctt gacgattttc
     5641 acgcgtaccc acaccgcgcc gaaatatccc aaaaccaaat cggcaatata ttccgccaac
     5701 gcttccaaaa gcaggaaatc ctgttctttc agatgtcggc gcagcgtttc gcatacctcg
     5761 gcataatgca ccgtattggc aatatcgtcg tccgaacccg ctttctcggg aacgccgatg
     5821 tccaaatcga caatcagggt ctgcaaccgt tcgcgctccc agccgtacac gccgataagc
     5881 gtatccgcct tgatgccgtg caaaaagatt ttatccataa tccgccccgt tttttgctaa
     5941 aataaccgca ccattctaag taaagtaccg caaatgttca acataccggc tgttgccgtt
     6001 tcctatctga tcggctcact ttctttcgcc gtcatcgtgt ccaaatatta cggcatggac
     6061 gacccgcgca cctacggatc gggcaatccg ggggcaacca atgttttacg cagcggcaaa
     6121 aaaaaggcgg ccgcgctgac gctcttgggc gatgccgcca aaggtttggt tgccgttttg
     6181 cttgcacgcg tgcttcaaga accgctcggt ttatccgaca gcgcaatcgc ggccgtcgca
     6241 ctcgccgcgc tggtcgggca tatgtggccg gtgtttttcg gatttaaagg cggcaaaggc
     6301 gtggcaacgg cattgggcgt gcttctggca ctctctccca caactgcctt ggtctgcgcg
     6361 ttgatttggc ttgtgatggc attcggcttc aaggtgtcct cccttgccgc attaaccgcc
     6421 acaatcgccg ccccccttgc cgcactgttt tttatgccgc atacttcttg gattttcgca
     6481 accctcgcaa tcgccatatt ggtgttgctc cgccataaga gcaacatcct caacctgatt
     6541 aaaggcaaag aaagcaaaat cggcgaaaaa cgctgattcg gcatttgttt tccattacgt
     6601 catgccgtct gaaaccatgt tcccgcgttc agacagcatt tccgcacaaa cggctttcat
     6661 accatgctca gttacagaca cgcattccac gccggcaacc acgccgatat gctcaaacac
     6721 tttaccttgt ttttagtgct gcaatatttc aaccgcaaag acaagcccta ctggtacatc
     6781 gacacgcacg gcggcgcggg tgtgtacaat ctcgaaggca gcgaggcgca gaaagtcggc
     6841 gaataccggc aaggcatcgc cctgctccga caggcgcaaa acctgcctgc cgaactctct
     6901 gactttgccg cgcacataca aaaaatcctg ccctcgcccg aactttactg cggttctccg
     6961 tggctggcac aatcgctgac ccgcgtcggc gacaaattac gcctgtttga gctgcacccc
     7021 accgactttg tccatctgca aaacaatatg ggcgaagcag gattgggcaa acgcggacaa
     7081 gtgttgcgcg aagacggtta caaagggctg atttccctgc tgccgccccc gccgcgccgt
     7141 gccgccgtcc tcatcgaccc gccttacgag gaaaaacagg actactggcg tgtaacggaa
     7201 acgctgaaat ccgctttaaa acggtttgag tccggctgtt atctcatctg gtatccctgc
     7261 ctcagccgcg aagaaagccg caagctgccc gaagaactga aaaaactcgt tcccgacaac
     7321 taccttcacg ccgaactgca cgtccacgcg ccgaaagccg acggtttcgg tatgcacggc
     7381 agcggaatgt tcgtcatcaa tccgccctat ctgttggcgg aacaattggc ggcaaacctc
     7441 cccgccctga cgcggctgct ggcgcaagac gaaggcgcgc gctacctttt ggacagcaaa
     7501 atccgctgac cccgatgccg tctgaaaccc ttcagacaac atctcccgtc aaataaacaa
     7561 aaccggacga tataccggca gtcggcactt ttaagaataa atatcccctc atccgcctgc
     7621 cgcgttccga cttgccccat gccgtttttt tacactacaa tcccctagac tatcctgcaa
     7681 aacgcaccaa aggaatacgc cacaataacc tgccgccttt cagacggcac aaacctttcc
     7741 gaaacgagca aactatggac acactgaccc gattcctccc cgaacacctg caacaaaacc
     7801 agctgcccga agcactcggc tgcgttttac tgtccgttgt ttccgcctgc accgaaatca
     7861 acgccaaagt ccgcctcggc gcactggccg gcgtattggg tatggcgggc acgggcaata
     7921 ttcagggcga agaccaaaaa aaactggatg ttatcgccaa caacatcatg attgacacac
     7981 tcaaagccaa ccctgccgtt gccggtttgg caagcgagga agaagactct ttcgtaagtg
     8041 ccggtgaaaa cggacgctat ctcgtcctat tcgatccttt agacggatcg tccaatattg
     8101 atgtcaacat ttccgtcggt acgattttct ccatccttga aaaacccgaa ggcgcattgg
     8161 caaccgaatc attccttcaa acgggcagac agcagcttgc cgctggttac gttctttacg
     8221 ggcctcaaac ccagctcgta ttcacattcg gacacggcgt atatatgttc acgctcaatg
     8281 ccgaaaacga atttgtgctg acaaaagaaa aaccgaaagt acccgaaagt accaaagaat
     8341 ttgccatcaa tatgtccaac cgccgccact ggctgccccc cgtccaacaa tacatcgacg
     8401 agctcttggc gggcgaaacc ggcacgcgcg gcaaaaacta caatatgcgc tgggtagcca
     8461 gcatggttgc cgaaatccac cgcatactga tgcgcggggg cgtgttcatg tatccgcaag
     8521 acaaacgcga cccgtccaaa cccggcaaac tgcgcctgat gtatgaagcc aatcctatgg
     8581 ccctgatact cgaacaagcc ggcgcatcgg caagcaacgc ccgtcaggac atactcggca
     8641 tccgtcccga aagcctgcac cagcgcgtcg ccgtcattat gggcagcagc gaagaagtgg
     8701 attacctcaa ccgcctgcat tcaaaataaa aggcagcctg accgataaag aaaggcggca
     8761 gcgcgcatcc gacgctgccg ccgatatttc gtttatagcc ttaaaaagat agaaaaccac
     8821 ctttccgaac ggcaaaaatg ccgtctgaaa agttttcaga cggcatttta aaaattaaag
     8881 cccgaattta tttgcccgct gctttttgca ggatggcgta caactcgtct ttcagtttca
     8941 atttggcttt tttcagctca tcgatggttt ccgcaccgct ggtaaccgga ttgttgacca
     9001 gaccggtaat tttatcgtcc agctcgttgt gttcgtcgaa cagacgggcg aagcgagaat
     9061 tttcctgttt caatttggaa atcaaatcac ggtattctgg gaacatatcg ctttcctctc
     9121 ttggtttacg gttaaaaagt caaattgaat ctttacatta attataacaa agataatatt
     9181 tttatccagt gttttatcgg atacggcaaa attttctttt aacaccgccg tccggttgat
     9241 aaaaacgttg acaatatcac gctttacgca taaattttca gcctttccta
//
LOCUS       DAWFIT010000078         9176 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFIT010000078 DAWFIT010000000
VERSION     DAWFIT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.078"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGQ85_001703"
     CDS             complement(254..2857)
                     /gene="pepN"
                     /locus_tag="WGQ85_001703"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0751079.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAMAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLGNAFKALLLGVPSEAELWEGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2993..3787)
                     /locus_tag="WGQ85_001704"
     CDS             complement(2993..3787)
                     /locus_tag="WGQ85_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0751080.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLYGINAVRYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4536..5405)
                     /locus_tag="WGQ85_001705"
     CDS             complement(4536..5405)
                     /locus_tag="WGQ85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0751081.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYRLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AVGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGQ85_001706"
     CDS             complement(5456..5992)
                     /gene="ruvC"
                     /locus_tag="WGQ85_001706"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0751082.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5995..6234)
                     /locus_tag="WGQ85_001707"
     CDS             complement(5995..6234)
                     /locus_tag="WGQ85_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0751083.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGQ85_001708"
     CDS             complement(6265..7275)
                     /gene="dusB"
                     /locus_tag="WGQ85_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0751084.1"
                     /translation="MRIGGYIIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFTDEGGVAAVQIAGSDPRQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSTAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
     gene            7643..9016
                     /locus_tag="WGQ85_001709"
     CDS             7643..9016
                     /locus_tag="WGQ85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0751085.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVTAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT         2137 a         2514 c         2335 g         2190 t
ORIGIN      
        1 gtattttaat taaccttaat cggtgcactt ctagcaatat agtggattca caaaaaccag
       61 tacagcgttg cctcgcctta ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
      121 tgatttttgt taatccacta taattaatat gactttgcgg ccgttttgcc attgcgtaat
      181 aaaacgatgg ggaagtgatg ataaaacgtg tgtgtaacta tatcagacgg cattgttttt
      241 ctgtttgacg gcctcaatcc aaaattttgc cgacgatttc gcccacgtct ttcgacaatc
      301 cttcctgcgc ccgaatgcgc tgcaatgctt gtttcaccaa gtttttgcgg tgcggctcga
      361 gcttgttgca gaggttgaac gcctgcacta agcgggcggc gacctgcggg ttaaagcggt
      421 cgatttcgat gactttgtcg gcgatgaagc ggtagccgct gccgtcttct gcgtggaaat
      481 gcgggacgtt gcggctgaag ctgccgataa gcgagcgggc tttgttgggg ttttcgaggc
      541 tgaatttcgg atgctgcaag gcagtttgaa cctgttgcag ggtgtcgctg cggcggcttg
      601 agccgacgag ggcgaaatat ttgtccatca ccagcgcatc atccgaaaac ttgtcggcaa
      661 actgagccag caggcggttg cgcgtatcgc tttcgttgcc gttgacggcg gacaggatgc
      721 cccattcgtg ggtcatgttt tgcgccattt cgccgtattt ttcggcaacg gtttcgatgt
      781 gcgcggggtc ggcgcgcagg acgaaggcgc ggcagacgtt gcgcagcgtg cgccagccgg
      841 cagcttcggg gctgtactcg tagctttggt tttcctgctt cgccgcctga cggttcaatt
      901 cgtgccattt cggcaggaag cggacggcga ttgtatccaa caaggcttcg tgcgcctgat
      961 ggtagcgcaa cgggtcgatg ttttccgtgc cctcccacag ctcggcttca gacggcacgc
     1021 ccaaaagcag ggctttgaag gcgttgccta agaggtcgtc tgaaatgact ttttcgacgg
     1081 cggcaagcag tttttcgtgt ttcggcagtt caataccgtc tgaaagtgcg gcaaggttgg
     1141 cggcaacggc acggcggtag agcgtttgtg cggcttccca gcgcgtgaag gcgtcgctgt
     1201 catgggcgag cagaagcagc aggtcgtcgt cgctgtacgg atagttcaaa tgcaccggcg
     1261 cgctgaaccc gcgcagcagc gagggaacga cggcttcggt tacgctttcg aactggaagg
     1321 tctgttcggc ttcggtcagc agtaacacgg cttcggttgc gcgtttgccc tgataatcga
     1381 atgccatcgc ttcgccgttg cggttcaaca gtccgacttt gacgggaatc atcatcggct
     1441 gcttgtccgc catatcgggc gtgggcggca cggtttgttt aatggtcaac tcgaaaatat
     1501 tgtttttcag acggctttcc gcttccaaaa cgggcgtgcc cgcctggctg taccacaagg
     1561 cgaactggtc gagattgatg ccgttcgcgt ccgccatcgc cgcgcggaaa tcgtcgcagg
     1621 tcacggcctg cccgtcgtgt cgttggaaat agagcttcat gcctttttgg aagccctctt
     1681 cgccgagcaa ggtgtgatac atccgcacca cttccgcgcc tttttcataa acggtcatgg
     1741 tgtagaaatt gttcatctcc tcatagctgg cggggcgcac cggatgggcg gtcgggcctg
     1801 cgtcttcggg gaactggtgc tggcgcagca ggcggatgtt ctcgatgcgg cgcacggcgc
     1861 ggctggcgcg gtcgccggaa aattcttggt cgcggaacac ggtcaaccct tctttcagcg
     1921 aaagctggaa ccaatcgcgg caggtaacgc ggttgcctgt ccagttgtgg aaatactcgt
     1981 gtccgaccac ggattcgatg ccttcaaaat cggtatcggt ggcggtacgg ctgtcggcaa
     2041 ggacgaactt ggtattaaaa atgttcagcc ccttgttttc catcgcaccc atattgaaat
     2101 cgcccacggc gacgaccatg aaaatatcca agtcgtattc caaaccgaag cgcgtttcgt
     2161 cccacttcat cgcgtttttc aaggattcca cggcaaagcc gaccttaggc ttgtccgcct
     2221 cggcagtgta aaactcgatt ttgacgtttc tgccgcccat cgtggtgaaa cagtcttccg
     2281 tgaccgccaa atcgcccgcg accaaagcaa acagatagct cggtttggca aacgggtctt
     2341 cccatttcac ccaatggcgg ccgtctgaac actcgccgcc gtcgattttg ttgccgttgg
     2401 agagcaaaac gggatagcgt tttttgtccg caacgatggt ggtcgtgaat ttggacatca
     2461 catccggacg gtcgatgtag aacgtgattt tgcggaagcc ctccggctcg cattgggtaa
     2521 acagattgcc accggaagca tacagcccca tcagcgattt gttttccgcc ggcaggatct
     2581 cggtttccac ttcgacggtg aagcgttcgg acggcacgtc tgcaatcgtc agagtctcgc
     2641 cttccaacac ataatccgcc gccacgccgt tgattttgac ggacaagagt ttcgccgaac
     2701 cgtccaacac cagcggctcc cctaccctct gcggctcaac cgtcaaacgg gatttcacga
     2761 cggtttgcgg ttcggcaata tcgaaatgca ggtcggtttc gagaatacgg taggcaggcg
     2821 tttggtaatc tttcagataa cggacggttt tgctcatttt tttctttcaa tattgtttgg
     2881 tttgactgga aaaggcttca gacggcatag gcaaaccctg caccgatatg ccgtatgaaa
     2941 ccgaaacatc agtgcggacg caccgtcaaa caaccggttt taatttcaat ctttatttcc
     3001 acgcttggac aaattctgcc caatgcggtt tgtcttctgt ttgggcggaa agatagtttc
     3061 tgatccgctt gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaaa
     3121 aacgcagata catcaaatac gtattcacgg catcggtttc acaataattg cggatttcct
     3181 tcaatttacc ggcatggaat gcctcccaga ccttgctgcc gtccataccc aatttacccg
     3241 gaaaaccgca cagtttcgcc atatcgtcca gcggcacatt cgccctcggc tggtaaagcg
     3301 cgagcaaatc catcaaatcg caatgacgtt ggtggtagcg gctgatgtag ttgttccact
     3361 tgaaatcgcg gctgtcgccg aaatcgccgt cgcccatatc ccaatagcgc acggcgttga
     3421 tgccgtacag caatgaacgg taatgcagga caggcaggtc gaaaccgccg ccgttccaac
     3481 tgaccagttg cggcgtatgt ttttcaatca actcgaaaaa tttggcaatc acgacttctt
     3541 cgccatcgtc catctcgccg attgtaccga catggacttt atcctgcccc caacgcatgc
     3601 agcacgaaat cgccacaatc tgatgaagat gatgctgcat aaaatcccca cccgtctgag
     3661 cacggcgttt ttgctgggca aacagcacca cttcatcgtc gggcagcgag gacggcagct
     3721 cgtacaatgt tcggataccc tgcacatcgg gtacggtttc aatatcgaaa gccaaaatcg
     3781 tggtcatgac agcaccttgt atttaaaaca gacttgcgcc tattgtgtca ttaaaaggcc
     3841 gataaaaaaa gaggcaaccc cccacaggat tgccccaata cctcaaatca gagatttacg
     3901 cttcacaaac aatacaggct ttcgcctgcg gctttacccg cgtagctcaa ctctacgccg
     3961 gcaaactttt gtttcaccgt ttccgatgaa accccgacca atcgcaagac tgaccggaaa
     4021 atcctttcaa acagcatttc ctgcctgtcg tgtaattcca tgtagcgaaa tgtacgccat
     4081 tttctacgct ttgccaagca ttttttacaa tataaatgtc aaaacattaa ttttataaaa
     4141 ttgctgaaaa tattaaatat atggattttt attttttata tttcaataaa tataaattta
     4201 attttgattt atatttaaat ttaagcataa aatgtcaaat attaaaggaa acatgaaagg
     4261 catatattaa atatttattt ataacgctat gtttttaaag aaaattaatt ttaatatatt
     4321 aactagattg tctgcatata ttcatgggtt tgcggtattt cttccaaaac ctgcttcgaa
     4381 tttcccgacc aagtcttaaa aatattgttt ttgagatact taaatagcag cgattatcaa
     4441 atgaaatctg ttcatataat ctgccatttt gcatttaaaa aaacaatcag gagtttcgac
     4501 tcgaaatgcc tgatatgttt tgtaatttta cgtagtcagt aaaaatcggg gctgccttcc
     4561 ggacgggttt taaaacgctt gtgcagccaa aaatactgct cgggatgttc gcgcacgcgt
     4621 tcctcgataa aacggttcat gcgctgcgcg tcggcttgcg catcttcact cggaaaggat
     4681 ttccaagccg ggtagaaatg cagtgtaact gtattgtctg cctcgcggac gggaatggcg
     4741 ggtatcactt ttgcatttgc aagcgcggca atgcggctca atccggtaat cgttgccgtc
     4801 tgaataccga agaaatccac aaaaaccgaa tcgttgcgtc cgaaatcctg atcgggcaga
     4861 tacagaaacg gcgcgctgct tttgcggaac tgtttgacga gggcgcgcag cccttcggtg
     4921 cgcccgataa ggaagacgtt gtgatagcgg ttgcgacctt tcaaaatctg ttcgtccaat
     4981 atcttgtttt tttgatggga atacatactg atcagcggga catcctgatt aagcgcgtac
     5041 accgccatct cgaacgcggt gaagtgcgga tacaggatga tgactttttc ccccaccgcc
     5101 agcgcgtcgt ccaaataatg cttattgcgg taacgcacca gcgatttcaa acgcccggca
     5161 ggcgcgtacc aatataaacc gtattccaac atcagtttcg ccatgtgttt gaaatgctgt
     5221 ttcaacacgg ttttacgctt ttcctcactc cattcgggaa aacattttgc caaattgatt
     5281 tcgccgatac ggcggcgcgg tttgaccaga cggtaggcaa gcaaacccgt caggtcggca
     5341 atcttatgca gcagcgcaaa cggcagaaac tgcaaaacat acagtacaaa aaatataaat
     5401 ttcatctcga tacacatttt cttttcagac ggcaaaatac aaatgccgtc tgaaactatt
     5461 gaaacctgcc gcgcttgacc tgcatccctg aaggattgag tttggcggca agcccgtggt
     5521 tgcgtaaggc gtgggtcagc gcgacggcaa gaccgtccgc cgcatccgcc tgcggcgttc
     5581 ccgaaagccc cagcatctgc accaccatat gctgcacctg ttctttcgcc gccttgcctt
     5641 tgccgactac cgcctgtttg acctgcaagg cggtgtattc cgaaacgggc agcttatggc
     5701 tgaccaatgc cgccaatgcc gcgcccctag cctgacccag catcagcgtc gatgcaggat
     5761 tgacgttgac gaacacctgt tccactgccg cctgttgagg cttgtaaacg gcaacgactt
     5821 cgccgatatg ccgcacaatc acggcaatcc tgtctgccag agacgcatcg gcaggcgttt
     5881 tgatgcagcc ggaggcaaca taaaaatgat cccgccccct gacatcgatg atgccgaaac
     5941 ccgttacgcg gctgcccggg tcaatgccca agatacggac ggttgccgac atattcacaa
     6001 caaaccgtgt tgaatcagtt ttttacgcaa ggtattgcgg ttcagcccca gcatcacgga
     6061 tgctttggac tggttgccgc cgcattgttc catcacgcac accagcagcg gtttttccac
     6121 ctgatgcaat accatatcgt acacgccgca aggttcggta ccgttcaggt ctttgaaata
     6181 ttgttccaaa ttttgtctga tacattggga aatatcggga agggtatggg gcataatcgc
     6241 gctttcaaaa ggataatcgg gtgttcagac ggcattttgg cggtaggcgc acgcccaacg
     6301 gtcggttttt ccggcaagtc tttcaagata acctgcaagc atgtcgtatt gcgccgccgt
     6361 actgtccaag cggttgattt cacgacgtgt ctgttcgccg tcgggcattt cgtcgatgta
     6421 ccagcctatg tgtttgcgtg caatgcgcac accggcggtc tcaccataaa acgcgtgcat
     6481 ggcgcggacg tggttcaaaa tggcggcact gcattctgcc aaactcaagg caggcggtaa
     6541 aacaccgtgt tcggcgtaat gtttcaaatc gcggaagaac cacggtctgc cttgcgcgcc
     6601 gcgccctatc atgatgccgt cggcggcggt ttgtttgagg acggcggcgg ctttttgcgg
     6661 cgaagtgatg tcgccgttga cccagaccgg gatgttcaga cggcatttgg tttcggcgat
     6721 gagttcgtaa cgcgcctcgc ctttgtacat ttgcgtgcgc gtgcgtccgt ggacggcaag
     6781 ggcggcgatg ccgcaatctt cggcgatttt ggcgatgacg ggcaggtttt gatggtcgtc
     6841 gtgccaaccc aaacgggttt tgagggtaac gggtacacct gccgcacgga cgacggcttc
     6901 caaaatggcg gcaaccagcg gctcgttctg catcagcgcg ctaccggctt ggacattgca
     6961 gactttttta gcgggacagc ccatgttgat gtcgataacc tgcgccccaa ggctgacgtt
     7021 gtaacgcgcg gcatccgcca tctgccgcgg atcgctgccg gcaatctgca cggcggcgac
     7081 accgccttca tcggtaaaat cactgcggtg cagggtcttt ttggtatttc tgagcgtcgg
     7141 gtcgctggtc agcatttcgc acaccgccca acccgcgccg aaatcgcggc aaaggcggcg
     7201 gaacggtttg tccgtaatgc ccgccatcgg ggcgagtgca atgggattgt cgataatgta
     7261 gccgccgatg cgcataatgg gtccgcgttt caaaaaagtg caacattgta cattttttaa
     7321 gcaggatttc caatctccgg acgcacccac gattggggcg ggcaccgttt tatggcataa
     7381 tccgcacaca gattccctgc cccgccactc acaggagggc agtttatagt ggattaacaa
     7441 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctcaag
     7501 caccaagtga atcaattccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     7561 tttttgttaa tccactatac tttccccgtc ctatcggttt cccgtttcag acgacataag
     7621 gtctgaaaga aagactacaa ttatgagtaa tccattttcc tctttaggtt tgggtacgga
     7681 actcgtttcc gcactgaccg cgcaaggtta cgaaaacccg acgcccatcc aagccgccgc
     7741 cattcccaaa gcactcgccg gtcatgattt actagccgcc gcgcaaaccg gcacgggcaa
     7801 aaccgccgcc tttatgctgc ccagtctgga acgcctcaaa cgttacgcca ccgccagcac
     7861 ctcgcccgcg atgcaccccg tgcgtatgct cgtgttgacc ccgacgcgcg aacttgccga
     7921 ccaaatcgac caaaacgtgc agggctacat taaaaacctg ccgctgcggc ataccgtcct
     7981 cttcggcggt gtcaacatgg acaaacaaac cgccgacctg cgtgccggct gcgaaatcgt
     8041 cgtcgccacc gtcggacggc tgctcgacca tgtgaaacag aaaaacatca gtttaaacaa
     8101 agtcgaaatc gtcgttttgg acgaagccga ccgtatgctg gatatgggtt ttatcgacga
     8161 catccgcaaa atcatgcaga tgctgccccg ccaacgccaa accctgctct tttccgccac
     8221 cttctccgcc ccgatacgca aactggcgca agacttcatg aacgcgcccg aaaccgtcga
     8281 agtcaccgcg caaaacacca ccaacgccaa cgtcgaacaa cacatcatcg ccgtcgatac
     8341 cattcagaag cgcaacctgc tcgaacggct gattgtcgat ttgcatatga accaggtcat
     8401 cgtgttctgc aaaaccaaac aaagcgtcga ccgcgtaacg cgcgaactgg tgcgccgcaa
     8461 cctgtccgca caggcgatac acggcgaccg ttcccaacaa agcaggctcg aaacactcaa
     8521 cgccttcaaa gacggcagcc tgcgcgtcct cgtcgccacc gacatcgccg cgcgcgggct
     8581 ggacattgcc gaactgccct tcgtcatcaa ttacgaaatg cccgcccagc ccgaagacta
     8641 cgtccaccgc atcgggcgca cggggcgcgc gggcgcggac ggcgtggcga tttccctgat
     8701 ggacgaatcc gaacagaaaa tgtttgaatc cattaaagag ctgaccggca acaagctgct
     8761 catcgagcgc atcgagggct tcgagccgca atggtgggaa cagggcggcg caaaaccgga
     8821 aaaaaccgaa atgcgcgaac cgagacaacg caaccgctac gaatccgcca gggctcagcg
     8881 cgaaaaaaat acccgaccgg aaaccacggc aaacgatgcg ggcgcggctt gcggaaaaat
     8941 tgccggacgc agccgccgaa gccgccggga acaccggacg tgcgccctgc tccaaccgcg
     9001 ttacggcgta aaataaccct gaaaatcaaa tgccgtctga acatttcccg tttcagacgg
     9061 catttttcaa actggactga cgcattgggc gcaaccgccc gcacaggata aatttcttcc
     9121 gcaaacagtt tcagacggca tttgccgcct gtacaatata gtggattaac aaaaat
//
LOCUS       DAWFIT010000079         9133 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFIT010000079 DAWFIT010000000
VERSION     DAWFIT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.079"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(72..1328)
                     /locus_tag="WGQ85_001710"
     CDS             complement(72..1328)
                     /locus_tag="WGQ85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0751086.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1676..2017)
                     /locus_tag="WGQ85_001711"
     CDS             complement(1676..2017)
                     /locus_tag="WGQ85_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751087.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2221..3078)
                     /locus_tag="WGQ85_001712"
     CDS             complement(2221..3078)
                     /locus_tag="WGQ85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0751088.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3139..3954)
                     /locus_tag="WGQ85_001713"
     CDS             complement(3139..3954)
                     /locus_tag="WGQ85_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0751089.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4198..4983
                     /locus_tag="WGQ85_001714"
     CDS             4198..4983
                     /locus_tag="WGQ85_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0751090.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5242..7364
                     /locus_tag="WGQ85_001715"
                     /pseudo
     CDS             5242..7364
                     /locus_tag="WGQ85_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            7488..9038
                     /locus_tag="WGQ85_001716"
     CDS             7488..9038
                     /locus_tag="WGQ85_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0751091.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVSAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEVDIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2296 a         2170 c         2410 g         2257 t
ORIGIN      
        1 tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta ttgcaaaggt
       61 ctcaaaccgg tttatttacg ttgcaatacg gcggcgaaga agccgtcggt ttggtgtcgg
      121 gcggagttga ggcgcaggta tttgccggta tccaaatcga ttttcaaacc ggcaagcagt
      181 tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc gacttgcagc
      241 tcgttttctt cgggcaggat gctgcaagtg gcgtacacca agcggccttg cggttttacc
      301 agtttggagg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc cacggtttcg
      361 gcggattggc ggtatttgag atcgggatta cggcgtaaag tacccaaacc ggagcagggc
      421 gcatccacta aaacgcggtc ggctttgcct gccagtcggg cgatacggct gtcgtgttcg
      481 ctgctgatgc gttcagggtg gatgttggtc agtccggcgc gggtcatacg cggtttgagg
      541 ttggcaaggc gtttttcggc gatatcgaag gcgtagattc tgcctttgtt cgccatttgc
      601 gcaccgacag ccaaggtttt accgccggca ccggcacaga aatcgacaat gatttcgccg
      661 cgttttgcgc ccaccaataa ggcaagcagc tggctgcctt cgtcttgaac ttccagcgta
      721 ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg gatgccccaa
      781 ggcgaataag gcgttgcctc tgcatcggca ctttcggctt gcaacaatgg cagcacttta
      841 tcgcgtttgc ctttcaaggt attgacgcgt atatcgagcg gcgcagcttg gttgatgctg
      901 cggccgaagg cgaggatttc ttcttcgcgc caatgctgct gcagctgttc taccagccat
      961 tgcggcaatt ctgcggcggt attcaggccg tctgaaaact cggttttacg ggctttcaaa
     1021 ttgccgagaa actctatttc ttcttcatca agcaggtctt tgatttggct gatgttggtg
     1081 cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg cggacggcgc
     1141 agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcggtttc ggcgatttcg
     1201 tggcggtctt ggcggccgag ctttttgtgt tcgcggaaat aggcggagag gacggcatcg
     1261 gaaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc gagttgtgcg
     1321 gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc taacgtcctt
     1381 aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag aaagatgtgt
     1441 taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg cagcctgaaa
     1501 aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag cgatttcatt
     1561 ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc gttcagtata
     1621 tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc tcttgttaaa
     1681 tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca ctctcattgg
     1741 ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt tcatttgtat
     1801 gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca tatatatctt
     1861 cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct tttttcagat
     1921 actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat gattgcttga
     1981 cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg gggtcgtagt
     2041 tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca tgttgttgtt
     2101 atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa ttgtatggga
     2161 aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg ggaaagggtt
     2221 tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc gacattccaa
     2281 aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg tttcggtttc
     2341 ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc ggacttcttg
     2401 gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac cgtatggatt
     2461 aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc gcacgatgga
     2521 aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc cgtctgaagt
     2581 gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg tgtctttggg
     2641 gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt tgcaggtcag
     2701 ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc cttgcgtatt
     2761 gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg gcgcgttggc
     2821 aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga acggggcgcg
     2881 tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa gatagcggaa
     2941 accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc acaaaggcgg
     3001 cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc accataatgc
     3061 gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa agctggaaat
     3121 gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg tattgaaaaa
     3181 accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca ggctctgcat
     3241 acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt tcatcacgct
     3301 ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt cttcttgttg
     3361 aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga cctgcacggc
     3421 ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca gtcggttgca
     3481 ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca gtgccacttt
     3541 ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg tttgcagcgg
     3601 cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt cggcaagggc
     3661 ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc cggaagcgag
     3721 gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa acacgggcgg
     3781 gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt tgtgcagacc
     3841 cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg tgcgcgtgag
     3901 gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag tcatgttcgg
     3961 ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga cggtcaaatg
     4021 ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa gcagacacgc
     4081 gtcaggcggt attttacccg atttggcagg atttacccca accaaataaa ctccgatttt
     4141 gccgctccga aggactgtac gtccgaaccg gcggtatgcc acccttaagg aaatctgatg
     4201 aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat gatgatacgc
     4261 gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag ccttcaacga ttttgtttcc
     4321 gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc ctatccgcac
     4381 cacaaaatca cttgtgaaga aagcggctcc cacggcaaag ccgcagccga gtacgaatgg
     4441 attatcgatc cgctcgacgg cacgaccaat ttccttcacg gtcatcccca atacgccatc
     4501 tctatggcgc tgctgcacaa aggcgtgttg caagaagctt tggtttacgc ccccgaacgc
     4561 aacgacgtat atatggcttc gcgcggcaaa ggcgcgttgc tcaacgaccg ccgcatccgc
     4621 gtttccaacc gcatcgaatt gaaccgctgc ctgatcggta ccggcttccc tgttgtcgat
     4681 caaagcatga tggacaagta tctggtgatt ttgaaagatt tcttggccaa aaccgccggc
     4741 ggccgtcgtg aaggcgcggc ttctttggat ttgtgtgctg tagcaacagg tcgttttgac
     4801 ggctttttcg agtttaacct caaaccgtgg gacattgccg caggtgcatt gattgttcag
     4861 gaagcgggcg gtatcgtaac cgatatgtcg ggtgaagacg gctggctgga aagcggcgat
     4921 attgtggctg ccaatcccaa agtgctggcg caaatgttga aaatcatttc cgcacacgtt
     4981 taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt ctgaagtatc
     5041 gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg gaaacggcta
     5101 aatgcccgaa actgccgagg atggaaacta aacggagaac cctgtgcaat ttgtttatag
     5161 tggattaaca aaaaccagta cggtgttgcc tcgccttagc tcaaagagaa cgattctcta
     5221 aggtgctgaa gcaccaagtg aatgggcaaa gccaacgcct ttaccgcgcc gattaccgtc
     5281 gtcaattacg acgaacaagc cctaaacaac accgaagcgc gtactttggt ggatgccgta
     5341 gcgaaaaaag acgcttccgt ttggcagttc ggcggcgaaa gcaacacgct gaccggactg
     5401 tatttcagag gttatcagct tgactcgcgc caattcagcg tcaacggttt ggcaggtatg
     5461 tacggcacgc aaggcacggc cagcgtgcaa gttggatcgg cacaactgat taaaggcgca
     5521 tccactgccg taaacggcat ggaccctgaa ggtgcggtat ccggttccgt caatatcgaa
     5581 accaaaaaag ccgccgatga aggcaaccgc aaaatcggtt tgggctggtt cagtaacaac
     5641 cgcgcccaag gtacgttcga cttgggccaa cgtttcggcg aaaacaaagc attcggcgtg
     5701 cgcgccaacg gcaaactgcg ccacggcgac accccgcgcc acggttacag cgaagacaac
     5761 aaagaatttg cggtaaatgc cgactatcgc ggcgaaacac tgcgcgtagc gttcgattcc
     5821 atctacgcaa aacgcaaaac caacggcggt cgcgcgcgta tgcaggacat tcaaaacgcc
     5881 ggcggacgct tgtttggcgc gcccgatggt aaaaccaacc tgctgccttc ttggaactgg
     5941 caaaataccg ccggccaaac caatatgctt acgtttgaat gggatgcgtt tgataatgca
     6001 cagattacag gcggtatcgg ctacaacaag gcacgctatt acggcacgct gatttccccg
     6061 accgtatgtt tgaatgctac cagcacttgt accgatatgc aaaacacaaa cgccagcggc
     6121 aaagtaacgg cacgcaacta tgactacaat accggtactg cgcgcctgac cgaccaatat
     6181 ttccgcacat tgagtatgaa cctgaccgca cgcggcgaat ttgaaaccgg tccggtaaca
     6241 cataactgga gcaccgcgtt cgaccgtgtt atccgccagc ggaaaaccat aaacggcagc
     6301 ggaaacggca acagcaagat agaagtgaag gcaaacgaaa atattgcgaa tcaattggca
     6361 tcgttcaaag cctactatcc caattcttgg gcgaaaaccg ccaacttgga tgccaatatc
     6421 aaagttaaca gcctggcttt gtccgacacc ttgggcttcg ttgataacaa ataccgcctg
     6481 actttgggcg gacgtttcca agcggttgaa tacaccgata agaaaaaatc acagagcggc
     6541 gatgccaaac gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt
     6601 gtgtacggca actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc
     6661 gaaacgacaa tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac
     6721 tggggcaatt tcgtaaccac gttaaacgcg ttccaaatca aacgcccggc tactggcgcg
     6781 gtcatacaac taccaagaca acaaaagggg taacaacaac gctgacctat ggcaacaatt
     6841 ccgatttcgc ccgctataag gcgcaaggcg gcgcggccgg cgacgagcaa ggtatggaac
     6901 gcaatcgcgg tattgagttc aacacctacg cgaacttgct caacaatact ttgcgtccga
     6961 ctttgggtct gatgtatctg caatcgaccg tgaaagagta tccaaattca cgcgatatgc
     7021 tggttaacgg cgtacaagtc gccaatccgc gcgtaattgc caaagcaggt ttggaatggg
     7081 atacaccgtt tgccaaagga ttgactttaa acggtaatgt ttcgtatttc ggcaagtctt
     7141 accaagacac gcaaaaacaa tacgccttcc cgtcctacac cttgattgac gtaggcgcgc
     7201 gctacaaaac caagctcggc aaaaatactt tgaccgtcag cagctcggtg gaaaacctgt
     7261 tcaacaaaaa ctattggcag gtacagcgcg gccaatacga ccgcagcttc gccgtcgtcg
     7321 gtatgccgcg cacttactgg ctgaaggcgg aattggattt ctgatttccc tatatccgga
     7381 aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agagatatgg cgggatacgc
     7441 ttaaataatg ctattctagc gtctttgaaa cctgcttgtt tgaagtgatg aaaaaacctt
     7501 tgatttcggt tgcggcagca ttgctcggcg ttgctttggg cacgccttat tatttgggtg
     7561 tcaaagccga agaaagcttg acgcagcagc aaaaaatatt gcaggaagcg ggcttcttga
     7621 ccgtcgaatc gcaccaatat gagcgcggct ggtttacctc tacggaaacg acggtcatcc
     7681 gcttgaaacc ggagttgctg aataatgccc ggaaatacct gccggacaac ctgaaaacag
     7741 tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc gccggcggat
     7801 tcggcacgca ggcgtacatt gaaaccgagt tcaaatacgc gcctgaaacg gaaaaagttt
     7861 tggaacgctt ttttgggaaa caagccccgg tttcccttgc caataccgtt tatttcaacg
     7921 gcagcggcaa gatggaagtc agtgtttccg ccttcgatta tgaagaactg tcgggcatca
     7981 ggctgcattg ggaaggtctg acggggaaaa cggtttacca aaaaggtttc aaaagctacc
     8041 gcaacagcta tgatgccccc ttgtttaaaa tcaagctggc agacaaaggc gatgccgcgt
     8101 ttgaaaaagt gcatttcgat tcggaaactt cagacggcat caatccgctt gctttgggca
     8161 gcagcaatct gaccttggaa aaattctccc tagaatggaa agagggtgtc gattacaacg
     8221 tcaagttaaa cgaactggtc aatcttgtta ccgatttgca gattggcgcg tttatcaatc
     8281 ccaacggcag catcgcacct tccaaaatcg aagtcggcaa actggctttt tcaaccaaga
     8341 ccggggaatc aggcgcgttt atcaacagtg aagggcagtt ccgttttggc acactggttt
     8401 acggcgatga aaaatacggc ccgctggata ttcatatcgc tgccgaacac ctcgatgctt
     8461 ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa atgactgagg
     8521 aacaaatccg caatgatttg attgccgccg tcaaaggcga ggcttctgga ctgttcacca
     8581 acaatcccgt attggacatt aaaacttttc gtttcacgct gccgtcagga aaaatcgatg
     8641 tgggcggaaa aatcatgttt aaagatttga agaaggaaga tttgaaccaa ttgggtttga
     8701 tgctgaagaa aaccgaggta gacatcagaa tgagtattcc tcaaaaaatg ttggaagact
     8761 tggcggtcag tcaggcaggc aatattttca gcgtcaatgc cgaagatgag gcggaaggca
     8821 gggcaagtct tgacgacatc aacgagacct tgcgcctgat ggtggacagt acggttcaga
     8881 gtatggcaag ggaaaaatat ctgactttga acggcgacca gattgatact gccatttctc
     8941 tgaaaaacaa tcagttgaaa ttgaacggta aaacgttgca aaacgaaccg gagccggatt
     9001 ttgatgaagg cggtatggtt tcagagccgc agcagtaatg ggtcaaatgc cgtctgaaat
     9061 gttcaggcgg cattttgttg tattggaaac tgccgttttc aaccattcct ttagataagc
     9121 cgtgtcggat tcg
//
LOCUS       DAWFIT010000080         9091 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFIT010000080 DAWFIT010000000
VERSION     DAWFIT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.080"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            218..997
                     /gene="pgeF"
                     /locus_tag="WGQ85_001717"
     CDS             218..997
                     /gene="pgeF"
                     /locus_tag="WGQ85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0751092.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1049..1528
                     /locus_tag="WGQ85_001718"
     CDS             1049..1528
                     /locus_tag="WGQ85_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751093.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1530..2528
                     /locus_tag="WGQ85_001719"
     CDS             1530..2528
                     /locus_tag="WGQ85_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0751094.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2649..3068
                     /locus_tag="WGQ85_001720"
     CDS             2649..3068
                     /locus_tag="WGQ85_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751095.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3144..3659
                     /locus_tag="WGQ85_001721"
     CDS             3144..3659
                     /locus_tag="WGQ85_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0751096.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3743..4627)
                     /locus_tag="WGQ85_001722"
     CDS             complement(3743..4627)
                     /locus_tag="WGQ85_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0751097.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5111..5974)
                     /gene="rpoH"
                     /locus_tag="WGQ85_001723"
     CDS             complement(5111..5974)
                     /gene="rpoH"
                     /locus_tag="WGQ85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0751098.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6238..7788)
                     /gene="lnt"
                     /locus_tag="WGQ85_001724"
     CDS             complement(6238..7788)
                     /gene="lnt"
                     /locus_tag="WGQ85_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0751099.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALIAALLAAGYT
                     ARQTDFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPET
                     AIPVMRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQD
                     GIPYYAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNI
                     CYEDGFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATN
                     TGATAIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALIL
                     FIFRNKEH"
     gene            complement(7857..8225)
                     /locus_tag="WGQ85_001725"
     CDS             complement(7857..8225)
                     /locus_tag="WGQ85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0751100.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8125..8583)
                     /locus_tag="WGQ85_001726"
                     /pseudo
     CDS             complement(8125..8583)
                     /locus_tag="WGQ85_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8777..9013
                     /locus_tag="WGQ85_001727"
                     /pseudo
     CDS             8777..9013
                     /locus_tag="WGQ85_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT         2001 a         2250 c         2668 g         2172 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact atataaagct cggagaaggt atataaggac
       61 atcattcctg caccggcaag aatccgaacc cgaacgtttg aaaacaatcc cgaatctccg
      121 aattcccgcc tgtgcgggaa tgacgaaaaa acaagcatcc atttgccccg aaggcagtta
      181 atcaaccctt tccgccacac acctattcca atatccaatg aaaaccatca cagaaaccct
      241 aaatctcgcc ccgaaaggca aaaacttcct gaccgccgat tggcccgcgc ccgccaatgt
      301 gaaaaccctg attaccacgc gcaacggcgg cgtgagccaa ggtgcgtatc agagtttgaa
      361 cctcggtacg cacgtcggcg acaatcccga agccgtgcgc cgcaaccgcg aaatcgtgca
      421 acagcaggtc ggattgcccg ttgcctacct caatcaaatc cacagtaccg tcgtcgtcaa
      481 tgctgccgaa gctttgggag gcacacccga tgcggacgct tccgtagacg acacgggcaa
      541 agttgcctgt gctgtcatga ctgccgactg tctgcccgtt ctattttgcg acagggcggg
      601 tacggcggtt gccgccgcac acgcgggctg gcgcggtttg gcgggcggcg tattgcaaaa
      661 caccattgcc gcgatgaagg ttccacccgt cgaaatgatg gcgtatctcg gtcccgccat
      721 cagtgcggat gcgtttgaag tcggacagga tgtgtttgat gcgttctgca cgcccatgcc
      781 cgaagccgcc gccgcatttg aaggcatagg cggcggcaaa ttccttgccg acctttacgc
      841 gctcgcccgc ctgattctga agcgcgaagg cgtgggcggc gtatatggcg gcacgcattg
      901 tacggttttg gaacgggata ctttcttttc ctaccgccgc gacggagcga cagggcgtat
      961 ggcgagcctg atttggctgg acggcaatgc cgtctgaaca cgccgctgat ataatctacc
     1021 gactttgtgc ttttgagaaa ggcaagccat gaacaaactg tttcttactg ccgcagtgct
     1081 gatgctgggc gcgtgcggtt tccacctgaa aggtgcagac ggcatttctc cgccgctgac
     1141 ctaccggagc tggcacatcg aaggcggaca ggcattgcag tttcctttgg aaaccgcgct
     1201 gtatcaggct tcgggcaggg tggacgatgc tgccggcgcg cagatgaccc tgcgtataga
     1261 cagcgtttcc caaaacaagg aaacctacac cgttacccgt gcggcagtta tcaacgaata
     1321 tcttttaatc ttgacggttg aagcgcaggt attgaaacgc ggcgagccgg tcggcaaacc
     1381 gatgaccgtg tccgtccgcc gcgtccttgc ttatgccgac aacgagatct tgggcaaaca
     1441 ggaagaggaa gcggcattgt gggcggaaat gcggcaggat gccgccgaac agattgtccg
     1501 ccgcctgacc tttctgaagg cggaatgacg tggcggcaca tatcggacac attgatacgg
     1561 acgcgccttt gaaacccctg tacgtcatcc acggcgagga agaactgttg cgtatcgagg
     1621 cattggacgc attgagggcg gcggcgaaga aacaaggtta ccttaatcgg gaagtttata
     1681 cggcagacaa tgccttcgat tggaacgaac tgttgcaaac cgcaggcagt gcgggtctgt
     1741 ttgccgattt gaagctgttg gaactgcata tccccaacgg aaaacccggc aaaaccggcg
     1801 gcgaggcgtt gcaggatttt gccgcccgcc tgccggaaga tacggtaacg ctggttttgc
     1861 tgcccaaact ggagaaaacc cagctccagt ccaaatggtt tgccgcattg gcggcaaagg
     1921 gggaagtgtg ggaagccaaa ccggtcggcg cggcggcttt gccccaatgg atacgcggac
     1981 ggctggacaa aatcggtttg ggtatcgggg cagacgcatt ggcactgttt gccgagcgcg
     2041 tggaaggcaa tctgttggcg gcgcgtcagg aaatcgacaa gctcgggctg ctgtatccga
     2101 aagggcatac cgtcaatatc gatgaggcgc aaaccgccgt tgccaacgtc gcccgcttcg
     2161 acgcgttcca actggcaggc gcgtggatga agggcgatgt cctgcgcgta tgcaggcttt
     2221 tggacggatt gcgggaagag ggcgaagaac cggtgctgtt gctgtgggcg gttgccgaag
     2281 acgtgcggac gctgatccgg cttgctgccg ccctgaagca ggggcagagt gtccaatccg
     2341 tacgcaacag tctcagactt tggggtgaaa agcagacgct cgcaccgctt gcagtcaagc
     2401 ggatttccgt cgtccgcctg cttgacgcgc tcaaaacctg cgcccaaatc gaccgaatca
     2461 tcaaaggtgc ggaagacggc gacgcatgga cggtattcaa acggcttgtc gtgtcgctgg
     2521 cggaataaag cggtaatccc caaaatccga aaatactgta aaataccgtt aatcctgaaa
     2581 agtattcacc aatccgtccg aaaacatttc agacggcacg accacctcaa taaaggaaca
     2641 ttaaccctat ggacaataag accaaactgc gcttgggcgg cctgatttta ctgactaccg
     2701 ccgttttaag cctcattatc gtattgattg tcgactcctg gccgcttgcc atcctgcttg
     2761 ccgccgtcat cgtcgccgcc gctgcgggcg gttttgtttg gacatcccgc cgacagcaac
     2821 gccagtttat cgaacgcctg aaaaaattcg acatcgatcc cgaaaaaggc agaatcaacg
     2881 aggcaaacct gcgccgtatg taccacagcg gcggacaaca ccagaaagat gcgattaccc
     2941 tgatctgcct gtcgcaaaaa tgttcggtgg acgaggcgca cgctatgttc aaaaaacgcc
     3001 cgacacgtca ggaaatcaat caaatggcgg caaaacagtc gcgcggtcag aaacgtccgc
     3061 accgttaacc gccgcaaggc atctttgcat aaatgccgtc tgaagcctgt tggcgtttca
     3121 gacggcatat tctgattgaa aagatgatga cactgaaaac cgccccgctc aaacgccgct
     3181 ttgccgccat gctgtacgaa atgctgctgg tcggtgcggc aacctgtttg gcagcattgt
     3241 ttgccggtat tgccgccatt tttctgaatc ccgtttctat cgcggtttct gcattggtaa
     3301 cgagtatcct gataatggga tcatggtggc tttattttcg cgccaactgg cacggtcagg
     3361 ggcagacctt ggcgatgagg acatggaaaa tcggcttgtg cgaccttaac ggcatacagc
     3421 cgtctttgca cctgctgcgc ctgcgcttta tttgggcgtg catattcatc gtcttcattc
     3481 ctatgcttgc ttatgccgga ttgcgccact tcctcggcat tccgcccaag ggcgcggtcg
     3541 gtgcggcatt gatttggctg attttaccgt gggggttcgc actgctgaat cccgatcggc
     3601 agtttctgta tgattttctt gcaggaacaa gattggtggc ggtcaaagga aagccttaag
     3661 cctttatacc gcaaaggttt caacctgaaa aaatgccgtc tgaaagggct ttcagacggc
     3721 atttgcttat cggggaaacc gattattcga tattctgcac ttgttcccgc atctgctcga
     3781 ttaagacttt cagttcgacc gaggcttggg tgcattcggc ggcaatggat ttgctgccca
     3841 aagtgttggc ttcgcggttt aattcctgca tcaggaaatc caagcgtttt ccgctgctgc
     3901 ctttgtgttc ggtaacgata cggcgcactt cggcaatgtg agtgcgcagg cggctgaatt
     3961 cttcgtcgat gtcggatttt tgaataaaga gggcaaattc ctgttgcagc ctgtcattat
     4021 tgatgtcgcc gactgcttcg acgaggcggg cgcggatttt ttctttatgt gtttccagca
     4081 gggtaggaaa gagttcgctc aatgcatcta tgatttcttc catgctttcg aggcgttgta
     4141 acaggtgctc gcccaatttt ttcccttcgc gccggcgtgc ggcagtaaag tcttttaacg
     4201 ctttttcggt cagttcggta atgctttttg ccaattcctc cgtattttcc ctttggcttg
     4261 ccaatacgcc ggggaaacgc aggatgtcgg caacgcccag ttttgccaaa tcgtgatgct
     4321 tgcggaggtc tttgttgatt tcggcaagct ggttcaccaa atccgagttg agttccaagg
     4381 actgtctgct gttttccgta tcttgaattt ggattttgca ttcgactttg ccgcgcgcga
     4441 tatgggatga aattttctcg cggataccgc tttccaaata gcgcaaatcg tcgggcatcc
     4501 tgatttgaat atctagaaaa cggtggttga ccgcacgcag gtcgaggttg atgcgtttgc
     4561 tgccgcactc tgccgccgcg ttggcaaagc cggtcatgct gtggatgtgg atatttccgc
     4621 tgctcatgtc gttctccgaa gcccgttaaa atggaatcaa tatatcacat ctgtatggcg
     4681 gcaagcgttt tcgggtgtga aaaattgaag gttttgcagc ggcagattgg aatcacgctc
     4741 ttttgttgct gcaaggaagg gaaatgtata gtggattaac caaaaccagt acggcgttgc
     4801 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt gaatcggttc
     4861 cgtacgattt gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat
     4921 atcaattccg ccaatctgtc ggaaaagcag ctgatgcggc agtgtctggt gcatgtctgc
     4981 tttttgattt cggcaattgc aacggcgtgg acggataaaa tcgtgtacgg cacgacgcac
     5041 aaaccgcatt gatgtttacc aaataaaata cccgacaaaa caatttgtcg ggtattttat
     5101 tgcgtatatt tcaaaccgct tcggcttctt cggtcaggaa accacgcagt ttctgcatgg
     5161 cttttgcttc gatttggcgg atgcgttcgg cagagacgcc gtattcggcg gcaagctggt
     5221 gcagcgtcag cccgccgtcg tcttgaagcc agcggctttc cacaatacgg cggctcctgt
     5281 catccagttg cgccaaagcg ttttgtaaac cttctgtttg cagggcgtaa tgcgcctgtt
     5341 tcgatagttg tcggctcggt tcggaatcgt ggtcggcaag ccagtcgatg ggggcgaaac
     5401 tgtcctcgtc gtcgctgttg tctgccatga tggcgatgtc gtgtcccgtc atgcgctgtt
     5461 ccatttccag aacttcggaa agtttgacac ccaaatcgtc ggcgatgtct tgtgcctctt
     5521 tgggagacag ggcgttgagg tttttacgca tactgcgtaa attgaaaaac agcttgcgtt
     5581 gcggtttggt ggtggcaacg cggacaaggc gccagttcct caggatgaat tcgtgaattt
     5641 ccgctttaat ccaatgcacg gcaaatgaaa acaggcgtgc acctcgacct ggctcgtagc
     5701 gtttgaccgc cttcatcagt ccgatattgc cttcctgaat caggtctgcc tgattcagcc
     5761 cgtagccgtc atagccgcgc gcgatggaaa cgacgacgcg caggtgggac aggatgagtt
     5821 gtttggcggc gttgaggtcg ccctttatcc ggcgttcggc gaggcgggtt tcttcttcct
     5881 gggacagcat cgggatgctg ttgacggtgt ggatgtattg ttcgaggctg ccgttgccgc
     5941 tttggatggc gggtaatgcg aaagcgttat tcatttgggg catttccttt cggctgaaac
     6001 tgcgtatcgg cggtttgctg tgttgggatg cagtatatca ctgcttggct tgtattttgt
     6061 atgtttggca ggagatatgc gctaaggttt gaaagacagg aaaattttgt aaggcaggtt
     6121 tgattgattt tgtaaacctg atggctcaat ccgatttcgg aattatatta catacgtagt
     6181 tgtatgtaaa cagccggttt gaaaaaagac agcccgtctg gacgggctgt gcaggtatca
     6241 gtgttctttg tttcggaaga tgaaaagaat cagtgcggct agggtcaata tgcccatcaa
     6301 ccaccatgaa ctgccggttt tcatataggg cgtttcgccg acatagcctt tgatgtgtcc
     6361 ttccaatacg gtttccgtat cgggttgggc ttgggcgatg atgttgcctt tgggggagat
     6421 gatggcggtt gcgccggtgt tggtggcacg gaccatatag cgtccgagtt ccatagcccg
     6481 tgcctgcgat tgttggaggt gctggtacat ggcgttggat tttccgtacc acgccatatt
     6541 gctggcattg gcaagcaggg tggcatcttt ggcggcggca atcagttcgt cgccgaatcc
     6601 gtcttcgtaa cagatgttga aggcgatttt ttggtttttc atcagcaggg cggattgctt
     6661 accgccgcct ttgcggaagt cggaaagggg catattcatc attttgtaaa gcggcgtggt
     6721 caggaagggc agcggtttgt attcgccgaa ggggacgagg tggtttttgg cgtagtaggg
     6781 gatgccgtcc tgattgtttt cctgataacc ggtcaggttg atgacggcgt tttcgtaacc
     6841 gttgccgtcc gaagtgtatt ggctgatgcc gacggcgagc gcgctgccgt tgttttgcgc
     6901 ctgttcggca aattttgcca gtatgttttc cggcaggttt tggcgcataa cggggatggc
     6961 ggtttcgggc aggatgacga tgtcggcagt ggttttgccg acctgttcgt aatatttctg
     7021 tatggtcggg atgacttggt cttcacgcca tttgagggtt tggtcgatgt tgccttgaag
     7081 cagggcgacg gtgctgcggc tgccgtcggg gcgggtgaag tcggtttgtc gggcggtgta
     7141 gcctgcggca agcagggcgg caatcagggc ggcaatcagg ataattggaa gcaggcgttt
     7201 gcccgaacgt gtggtgttat cactcgccaa aaccagccag acaccgagaa aggcggttgc
     7261 cagtgtaacc atgtggatgc cgcccaatgg ggcaaagccg gcgagcgggc tgtccggggt
     7321 gatttgggag tagccgattg cgccccagcc gaatccggtc aggaaacgtt cgcgggcaaa
     7381 ctcggtcagc gtccacagga tgggcagtac caaaccgatt tttatgcccc gaggcagggt
     7441 aaattttttc cacagccaga aacacagtgc cggataaagg gcaaggtagg cggggagtag
     7501 gaaggtcagc ggtacggcat agaggtcggg caggccggaa acgtcgtgca gggcggtgtg
     7561 tatccagtag aactgtgtcg tgtatgcggt caggccgaac aggtaggcgg aagagacagc
     7621 aaaacgcgga cgcagttcga tgaggcggac gaaggcaccg aaaatcaagg gcatcagcca
     7681 aaagtggtag tagggtgcga aggtaaaggg ggtggcggcg gcaaaaagga tgagcaaagg
     7741 ccagtagagg gcggggtgct gccagtattt gtccagtttg gaaaccatat tcatctgtct
     7801 gttcggaaga taccgtctga acatctttca aacggcatcg gtatttgaaa aagaaatcaa
     7861 tgcctgccga aacgattcat cagcggcaag gcgggggcgc aggcaatcga accggaaagc
     7921 agcccgacaa cgaggttggc gaagtactcc gcccagccag cgtcccagtg ttgcgcgtgc
     7981 aggaaatcgt gcagaaggcc gaggttgtgg gcgaccagta cgccgccgat aagaaacatg
     8041 gcaagcgtgc cgaccacgct caaaccgcgc ataaagcaag gcataaaggc agtcagcatt
     8101 tgccccaaac tgcgcgaaaa ggtttgtggg cggcgcatca gcagcatgcc taagtcgtcg
     8161 agtttgacga tgacggcaac gattccgtac accaaaacag tcatgccgat gccgattgcc
     8221 gccattacga gcatgcgcgt catgtcggtg cagaaacttg tgcagcagct tttctacgcc
     8281 gtcaaagcac agataaatgc cgcctgccgt caaaagcggc gtaatgagtt gcggcaggaa
     8341 ggcggaaagc agcagagccg caggtaccaa aaccggcttg ttggaaaaag aacctttcgc
     8401 catcgaccaa acaatcggca actcgcgttc tgccgatacg cccgtaaccc ggttggcatt
     8461 gggtgccaaa tcgtcgccga ccatgccggc ggttttcttt gcggcggctt tggtcatcag
     8521 ggcaacatcg tccaaaacgg cggtgatgtc gtccggcagg gtaaatagtg aggcaaatgc
     8581 cattaaagaa tcctgaaatg cggcgcaaag tccgacatta tataggagaa cgcggatttg
     8641 ggcggtttca ggcggcatga aacaggaaaa tgccgtctga aggcggtggc ggacgtgaag
     8701 taaagtttcg tgaaaaaaat gccgtgttac agtctttcga ttttaattta atgaatttta
     8761 agggagaatc gttagcgtgg attggatggg cagtctgttc ctgccgggtg gcgcactgtt
     8821 gtttctgagc gtggtttcga ccactttacc cgcacgtttg ggaatgcctt tgctgctggt
     8881 ttctcccgcc ggcgtgttgg acggggcggc ggaagccttg gcgattgcgg cgttcctgat
     8941 gctggtcgcg cgtccgccgg cagtgttcgg cggtttgtgg aaattcaatt acagcctgcg
     9001 tgaaaaggcg tatagccgaa tcgaaatgca gtccgacacc gtgcttcagg cggggatttg
     9061 gtgtggtaca tcctgcccga cggcaaggtc g
//
LOCUS       DAWFIT010000081         8824 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFIT010000081 DAWFIT010000000
VERSION     DAWFIT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.081"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGQ85_001728"
     CDS             complement(289..1185)
                     /gene="argB"
                     /locus_tag="WGQ85_001728"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0751101.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1355..2485
                     /locus_tag="WGQ85_001729"
     CDS             1355..2485
                     /locus_tag="WGQ85_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0751102.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2556..3407
                     /locus_tag="WGQ85_001730"
     CDS             2556..3407
                     /locus_tag="WGQ85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0751103.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3490..4152)
                     /locus_tag="WGQ85_001731"
     CDS             complement(3490..4152)
                     /locus_tag="WGQ85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0751104.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            4186..4293
                     /locus_tag="WGQ85_001732"
                     /pseudo
     CDS             4186..4293
                     /locus_tag="WGQ85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4352..6145)
                     /locus_tag="WGQ85_001733"
     CDS             complement(4352..6145)
                     /locus_tag="WGQ85_001733"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0751105.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAKEYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNVAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6391..7500
                     /gene="proB"
                     /locus_tag="WGQ85_001734"
     CDS             6391..7500
                     /gene="proB"
                     /locus_tag="WGQ85_001734"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0751106.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIARIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7513..8775
                     /locus_tag="WGQ85_001735"
     CDS             7513..8775
                     /locus_tag="WGQ85_001735"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0751107.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         2011 a         2483 c         2297 g         2033 t
ORIGIN      
        1 ccgataagtt tccgtgccga caggtctaga ttcccgcctg cgcgggaatg acgaaatttc
       61 aaagttatgg cgttatcgga aaaacagaaa atcaaagccg gagaatttat cccaaacaac
      121 cggatttcaa aaaaaccaga ttcccgcctt cgcgggaatg acggatttta ggtttctgtt
      181 ttggtttctg ttttcgcggg aataacaact tagaaattgc ccgaaacccc aaaaacagaa
      241 accggacaaa cggggtttcc gccaaagccg gcattttccg actttgcttc aggcatcttc
      301 cccaccgccc aaaatcatcg aaccgatacc ggcatcggta aagatttcca gcaaaagcgc
      361 gttgggcacc ctgccgtcga tgatgtgcgt ggctttcacg ccgttgacgg cggcttcgac
      421 cgcggaagcg attttcggca gcataccgcc atacagcgta ccgtcggcaa tcagttcatc
      481 aatccgtttc ggcgtgagtt tggtcagcag attgcccgtt ttgtccatca caccggcgat
      541 attcgtcatc atcaaaagtt tttcggcgtt caattcttcc gccaatttgc ctgccaccaa
      601 atcggcgttg atgttgaacg cttcgccttt ttcacctacg ccgacggggg cgacgacggg
      661 aatgcagcca cgttctatca gccctttaac caaaccggta tcgatgcttt ccaccgtacc
      721 gacctgtccg atgtccacgc cattctgttc gggcgtatca atcaaaagtt tcttcgcctt
      781 aatgaaatgg tcgtcgcgtc cgcttacgcc gaccgcgtgt ccgccatatg tgttaatcat
      841 cgacacgatt tctttattga catgcccgcc caacaccatt tcgacaatat ccatcgcctc
      901 tttgtcggta acgcgcattc cttggacaaa ctcacccttt ttgccgactt tttcaagcat
      961 cgcattgatc tgcggcccgc cgccgtgaac gatgacggga tgaatgccga ccagcttcag
     1021 cagcacgaca tcgcgggcaa acccttcttt caaggcaggt tcggtcatcg cgttgccgcc
     1081 gtatttgatg acggcgaccg aaccggaaaa ccggcggatg taaggcagcg cttcggcaag
     1141 gatacgcgcc ttgtcggcgg cggaaataat gttttcagac tccataacgg ctccctattg
     1201 ttgacaattt cggcttatgt taacgccaaa cgccgtctga aacaatcttc agacggcatc
     1261 cggcagactt tcgggcaggt tgaagccttg cgatacgggc ggtaaaatcg cggtttatcc
     1321 ggttcgagaa accggcgaca gcaaggactt tcccatgatt aaaatcagca cccgcttcga
     1381 tgccggctcg gtcgtcgtca aagacctgac cgatccttcc aacatccgcc tcgccctgcg
     1441 tcccgacaac gcctccgatt ttgcacaatg gttctacttc cggctgcaag gcgcggcata
     1501 tcaaaactgc atcatgcatt ttgaaaacgc ggcagaagcc gcctacccga aaggctggga
     1561 aggctatcag gcgtgtgcct catacgaccg ccgcaactgg ttccgcgtac cgacttccta
     1621 cgaaaacggc gtactgaccg tcaatcatac gccgctgtcc aacagcgtgt attacgccta
     1681 tttcgaacct tactccgaag aacagcattt aaacctcctc ggcgacgcgc aaggcagcgg
     1741 cctgtgccgc atcgacgact tgggcagcac cgtgcaaggg cgcgacatca atctgctgac
     1801 catcggcaat caggtcgaaa gcgatctgaa aatctggatt accgcccgcc agcatccggg
     1861 cgaaaccatg gcggaatggt ttgtcgaggg gctgctcggc aggctgctcg atccgcaaga
     1921 cccgacagcg cgcaccctgc tcgaccgcgc cacgttctat atcgtcccca atatgaaccc
     1981 cgacggttcg gcactgggca accttcgtac caacgccgcc ggtgcgaacc tcaaccgcga
     2041 atgggaaaat ccgactttgg aaaaaagccc cgaagtgttc ttcgtgcgtg aaagaatgct
     2101 ggaaaccggc gtggatttgt ttttggatat ccacggcgac gaagggttgc cctttgtctt
     2161 tgttgcagga actgaaggcg tgccgaacta caatccgcgc atttccgcgt tggaaacaca
     2221 gttcaaaacc gcccttttga acgccagccc cgatttccaa gacgaatacg gttacgaaaa
     2281 agatgcgccc ggtcaggcaa acatgacctt ggcaacaaac tgggtcggca accgtttcaa
     2341 ctgccttgcc tatacgttgg aaatgccgtt caaagacaat gccaacctgc ccgacgacga
     2401 tttcggatgg aacggtcaac ggtcgttgcg cttgggcgaa gccgcgttgt ccgccatcct
     2461 caacatcatc ggcgatttgc gctgaaaccc ttattgcaga aatgccgtct gaagcatcat
     2521 gcctttcaga cggcatttga aaacgaaagc ccgttatgat tacccatccc caattcgacc
     2581 ccgtccttat cagtatcggc ccgcttgccg tccgctggta tgccctaagc tacatcctcg
     2641 gatttattct ttttaccttt ctcggcagaa ggcgcatcgc gcaaggcttg tccgttttta
     2701 ccaaagaatc gctcgacgac ttcctgacat ggggcatttt gggcgtaatt ttgggcgggc
     2761 gtttgggtta cgtcctgttt tacaagtttt ccgactacct cgcccatccg cttgatattt
     2821 tcaaggtatg ggaaggcgga atgtcgttcc acggcggctt tttgggtgta gttattgcca
     2881 tatggttgtt cggccgcaaa cacggcatcg gcttcctcaa actgatggac acggtcgcac
     2941 cgctcgttcc gctgggtctc gcttcgggac gtatcggcaa cttcatcaac ggcgaacttt
     3001 ggggacgcgt taccgacatc aacgcatttt gggcaatggg cttcccgcag gcgcgttacg
     3061 aagatgccga agccgccgcg cacaatccgc tttgggcaga atggctgcaa caatacggta
     3121 tgctgccgcg tcatccctcg cagctttatc agtttgcact tgaaggcatc tgcctgttcg
     3181 ccgtcgtttg gctgttctct aaaaaacagc ggccgaccgg acaagtcgcc tcgctcttcc
     3241 tcggcggcta cggcatattc cgcttcattg ccgaattcgc acgccaaccc gacgactatc
     3301 tcgggctgct gaccttgggg ctgtcgatgg ggcaatggtt gagcgtcccg atgattgttt
     3361 tgggtatcgt cggctttgtc cggttcggca tgaaaaaaca gcactgaccg cctgtgaaaa
     3421 ataccgtctg aaacatggaa atccggtttc agacggcatt tttacgtttg gttcatggaa
     3481 acaaacgctt caattcttac cgcaacacgc cttgtatttt ctgccgctgc cgcacggaca
     3541 gggatcgttc ctgcctgttt tttcgccttc cctgcggacg gtttgcggtt tgttgatgac
     3601 tgcctgccag tagcggtaga tatccgccaa tgcataaggc aactcggatt ccagttccgc
     3661 cagctcgcct tctgtgaatt gcagacggat agcgccgttt tcctcttcgt cgtaaatacc
     3721 gcccaatgcc atgatgggat aaaacaactc ttcaaacgct tcatcatcga cggcttcaaa
     3781 ccaatcggtc ggcacaatat ccaaaccgta aagataagca ttgcaccatg tgtaaaaatc
     3841 gctgccgccg tcttcgtttt catacagcca caaatcgggc agttttttat ccgacatcgc
     3901 ggcggttgtt tccatcgcca ttgccaaaac cagccgttcg atttcggaac gttcggcggc
     3961 ggtaaattgc gattcgtcgc ccaacacttc gggcagccag tcgagcggtg tcaatttgtc
     4021 cggcccgctc aacagcgccg tcataaaacc ttgaacctcg tcgcaacgca tcgtgttgcc
     4081 ttgttcgctt ttggcatcca acaattcgct caaccgccgt ttggatgctt cggtaaattt
     4141 tcgggaatcc atcattttcc ttttcaaatg ggttttgcgc cctattatag tggattaaat
     4201 ttaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
     4261 acgccgtact ggtttaaatt taatccacta tatcgccgca atgccgtctg aagccttttt
     4321 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4381 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcagtggcaa
     4441 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgt gccagacgga
     4501 cgctggtttc gccttggctt tccgtacctt gcgtgacggc attgacggaa taaatctgca
     4561 aagtcgcgcc gctttgcgcc acgctttcaa tcgctttgaa aattgcatca accgggccgg
     4621 aaccggttgc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4681 gctcttcgcc ggtttcggta ctgatttttt gggaaatgaa tttgtagctt tcggtattca
     4741 tattgcccat ttcgtccgac accagcgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4801 tgtcggcgag ttctttgaag cgtgcaaatg ccacgttcag tgcctcttcg ctttccaact
     4861 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4921 tcaatcggtt agccgaccaa ccgaccgatt cggcagacat aatctcgtaa gtttcgcggt
     4981 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     5041 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     5101 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     5161 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     5221 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     5281 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5341 gttcgcggaa aaattcttcg gttttgtacg ggatggaata gccgacggta tcgggaatat
     5401 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcagcaagg aaatcgattt
     5461 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattct ttggcgattt
     5521 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5581 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5641 cctcccccgc ctgacggatg tcccgctcga tggcgcgtga caatgaacag accgttgatt
     5701 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5761 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5821 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5881 tgataacgcg gttggcctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5941 aagcattcaa atccagcggc ctgccttgcg attcggcaag cgcggtcagc ttttgcagtt
     6001 gcgccaaagg cagcgacccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     6061 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     6121 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     6181 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaaatc
     6241 tcaataaacc gccatagatt ttattaattt tatacatttt gtccatttta ttgcagacgc
     6301 ggcgtgattg ttgacaattg cggcagaaca gggcaaattc ggcggcacaa ggtggcaacg
     6361 agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt atttaaagtc
     6421 ggcacatctt cgattaccca ttcggacggc agtctctcgc gcggcaaaat ccaaaccatc
     6481 acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt gtcttccggc
     6541 gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac gtccggtcaa aatcgccgac
     6601 aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac ggcaaacctg
     6661 tcttcagacg gcatcgtgtc cgcgcaaatc ctgctcagcc gcgccgactt tgccgacaaa
     6721 cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc tgcaacgccg cgccgtcccc
     6781 atcatcaatg aaaacgatac ggtttcggtt gaggaattga aaatcggcga caacgacaca
     6841 ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct gaccgacata
     6901 gacggtcttt acacgggcaa cccgaacagc aatcccgatg ccgtacggct ggacaaaatc
     6961 gaacacatca accatgaaat catcgaaatg gcgggcggat cgggttcggc aaacggcacg
     7021 ggcggtatgc tgaccaaaat caaagcggca accatcgccg ccgaatccgg cgtaccggtg
     7081 tatatctgtt cctcgctcaa acccgatgca cttgccgaag ctgccgaaca tcaggcggac
     7141 ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc agaagcaatg gctggcgttc
     7201 tattccgaaa gccggggcag cgtttatgtg gacgaaggtg cggaacacgc tttgtccgaa
     7261 cagggaaaaa gcctgctgat gtcgggcatt gccagaatcg aagggcattt ttcccgtatg
     7321 gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg gcgcgtcctg
     7381 ttcggttctg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg cgtgttcatc
     7441 catcgggacg actggatttc catcacgccc gaaatacgcc tgcttctgac cgaattttag
     7501 aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc ggcaaaaaaa
     7561 tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc tgcttgctat ggcggacagt
     7621 ctggaagcgg cggcggcaga tattttggcg gcaaaccgtc aagacttgga agccgcggca
     7681 ggcaacattc ccgaaagcat gaccgaccgc cttttgttgg acggcaaacg catttgcgcg
     7741 atggcggacg gcattagggc ggttaccgca ctgcccgacc ccgtgggcga aatactggaa
     7801 acctcgactc tgcccaacgg cttggaaatc gtcaaaaagc gtgtggcgat gggcgttatc
     7861 ggcattattt acgaaagccg cccgaacgtt acgtccgatg cggcggcttt ggcactgaaa
     7921 agcggcagcg cggtcgtact ccgcagcggc aaagatgcat tccaatccgc acgcgccatc
     7981 gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgacgc gctccagttg
     8041 attgaagaca ccgggcgcga aagcagttac gaaatgatga gggcgaaaga ttatctagac
     8101 ctgctgattc cgcgcggcgg ggcggggctg atacgggcgg tggttgaaaa tgccgtcgtg
     8161 ccggtcattg aaacgggaac gggcatcgtc cacatttata tcgacaagga cgcggattgg
     8221 gacaaggcgc tccgtatcgt gtacaacgcc aaaaccagcc gcccgtccgt gtgcaactcg
     8281 atggaagtgc tgctggtgca tgaagacatt gctgccgact tcctgcccaa gctcgaacgg
     8341 ctgttggttc gcgaccgcat agaagccgga ctgccgcccg tccgcttccg tttggatccg
     8401 caggcggcgc ggcatatcgg cggcgaagcg gcgggtgcag acgatttcga taccgagttt
     8461 ttagactaca tcctcgctgt gaaaaccgtc gcatcggtcg aagaggcggt cgggcacatc
     8521 gaagcccacg gcacacacca ttcagacggc atcgttaccg aaaaccgcca cgctgcggac
     8581 tatttcacga cccatatcga ttctgccgcc gtgtatgtca acgcgtccac gcgctttacc
     8641 gacggcggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa actccacgcc
     8701 cggggcccga tgggtttgaa agagctgacg agttacaaat acatcgtaca aggcacggga
     8761 caggttaggg aataatccga attcaaacgt ctgatatagt ggattaactt taaaccgata
     8821 cggc
//
LOCUS       DAWFIT010000082         8752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFIT010000082 DAWFIT010000000
VERSION     DAWFIT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.082"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            150..1319
                     /gene="prpF"
                     /locus_tag="WGQ85_001736"
     CDS             150..1319
                     /gene="prpF"
                     /locus_tag="WGQ85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0751108.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1733..2932
                     /locus_tag="WGQ85_001737"
     CDS             1733..2932
                     /locus_tag="WGQ85_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0751109.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            3290..3910
                     /locus_tag="WGQ85_001738"
     CDS             3290..3910
                     /locus_tag="WGQ85_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0751110.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(4037..4480)
                     /locus_tag="WGQ85_001739"
     CDS             complement(4037..4480)
                     /locus_tag="WGQ85_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0751111.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4654..5022
                     /locus_tag="WGQ85_001740"
     CDS             4654..5022
                     /locus_tag="WGQ85_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0751112.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5067..6221
                     /locus_tag="WGQ85_001741"
     CDS             5067..6221
                     /locus_tag="WGQ85_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0751113.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6278..7150)
                     /locus_tag="WGQ85_001742"
     CDS             complement(6278..7150)
                     /locus_tag="WGQ85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0751114.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7235..8047
                     /locus_tag="WGQ85_001743"
     CDS             7235..8047
                     /locus_tag="WGQ85_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0751115.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1907 a         2379 c         2521 g         1945 t
ORIGIN      
        1 tttaataatc tgccgtatca caacacagta gccgtagatt gtggcgaacc ccgacagttt
       61 gcggaatcaa acggctttgg tcggagtggc agcctaatcc actataaaaa tcgtgggcag
      121 agcccacgct acataaggag aatctagaaa tgccgcaaat taaaatcccc gccgtttact
      181 accgcggtgg cacatcaaaa ggcgtgtttt tcaaacgttc cgatctgcct gaggcggcgc
      241 gggaagcggg tagcgcgcgc gacaaaatcc tgttgcgcgt actcggtagc cccgaccctt
      301 atggtaaaca gattgacggt ttgggcaacg ccagttcgtc caccagcaaa gccgtgattt
      361 tggacaagtc cgaacgcgcc gatcacgatg tcgattacct tttcgggcaa gtttccatcg
      421 acaaaccttt tgtcgattgg agtggcaact gcggcaacct caccgctgcc gtgggcgcat
      481 tctccatcga gcaaggcttg gtcgataaag gcaagattcc ttcagacggc atctgcacgg
      541 tcaaaatctg gcagaaaaac atcggcaaaa ccattattgc ccatgtaccg atgcaaaacg
      601 gcgcagtttt ggaaacaggc gattttgagc tcgacggcgt aacgttcccg gcagccgaag
      661 tacaaatcga atttcttgat ccagccgacg gcgaaggcag tatgttccca accggcaatt
      721 tggtcgatga aattgatgtg ccgaatatag gccgtttgaa agccacgctc atcaacgcgg
      781 gcattccgac cgttttcctg aatgccgccg acttgggcta cacgggcaaa gagttgcaag
      841 acgacatcaa caacgatgcc gcagctttgg aaaaattcga gaaaatccgc gcttacggtg
      901 cgctgaaaat gggtctgatc agcgacgtat ccgaagctgc cgcccgcgcg cacacgccga
      961 aagtcgcctt cgtcgcgccc gccgccgatt acaccgcctc cagtggcaaa accgtgaatg
     1021 ccgccgacat cgatttgctg gtacgcgccc tgagcatggg caaattgcac cacgcgatga
     1081 tgggtaccgc ctctgttgcc attgcgaccg ccgccgccgt gcccggtacg ctggtcaacc
     1141 ttgccgcagg cggcggaacg cgtaaagaag tgcgcttcgg gcatccttcc ggcacattgc
     1201 gcgtcggtgc agccgccgaa tgtcaggacg gacaatggac ggccaccaaa gcggttatga
     1261 gccgcagcgc acgcgtgatg atggaaggtt gggtcagggt gccggaagat tgtttttaaa
     1321 ttgacgtagc atgggtttgc ccgcgagcca taaacaggtc gtctgaaaaa caagtaaaca
     1381 tcaaatcact gaccattcct ttcccttgcc ctgtggcgga aggcggcaaa tcacaaggaa
     1441 gaacacggaa accccgataa aagacagctt cccgtattac cgtcattccc gcgcaggcgg
     1501 gaatctagac ctgtcaatat ggaggattgg taggggaaaa caggtttcgt gagttctaca
     1561 ttctggattc ccgccacagc ctgtcctcgc gtaggcgggg acggaataac gatagaaaat
     1621 gcggcatacg ctttgcccaa agaggccgtc tgaaacacct tgcgcctgat gtctgccttt
     1681 ttcagacgac cccacaccaa aaaaacaacc acaaactaca aggagaaaca tcatgtccga
     1741 ccaactcatc ctcgttctga actgcggcag ttcatcgctc aaaggcgccg ttatcgaccg
     1801 aaaaagcggc agcgtcgtcc taagctgcct cggcgaacgc ctgaccacgc ccgaagccgt
     1861 cattacgttc aacaaagacg gcaacaaacg ccaagttccc ctgagcggcc gaaattgcca
     1921 cgccggcgcg gtgggtatgc ttttgaacga actggaaaaa cacggtctgc acgaccgcat
     1981 caaagccatc ggccaccgca tcgcccacgg cggcgaaaaa tacagcgagt ctgttttgat
     2041 cgaccaggcc gtaatggacg aactcaatgc ctgcattccg cttgcgccgc tgcacaaccc
     2101 cgccaacatc agcggcatcc ttgccgcaca ggaacatttc cccggtctgc ccaatgtcgg
     2161 cgtgatggat acttcgttcc accaaaccat gccggagcgt gcctacactt atgccgtgcc
     2221 gcgcgagttg cgtaaaaaat acgctttccg ccgctacggt ttccacggca ccagtatgcg
     2281 ttacgttgcc cctgaagccg cacgcatctt gggcaaacct ctggaagaca tccgcatgat
     2341 tattgcccac ttaggcaacg gcgcatccat taccgccatc aaaaacggca aatccgtcga
     2401 taccagtatg ggtttcacgc cgatcgaagg tttggtaatg ggtacacgtt gcggcgacat
     2461 cgatccgggc gtatacagct atctgacttc ccacgccggg atggatgttg cccaagtgga
     2521 tgaaatgctg aacaaaaaat caggtttgct cggtatttcc gaactttcca acgactgccg
     2581 caccctcgaa atcgccgccg acgaaggcca cgaaggcgcg cgcctcgccc tcgaagtcat
     2641 gacctaccgc ctcgccaaat acatcgcttc gatggctgtg gcctgcggcg gcgttgacgc
     2701 actcgtgttc accggcggta ttggcgaaaa ctcacgtaat atccgagcta aaaccgtttc
     2761 ctatcttgat ttcttgggtc tgcacatcga cactaaagcc aatatggaca aacgctacgg
     2821 caattcgggt attatcagcc cgaccgattc ttctccggct gttttggttg tcccgaccaa
     2881 tgaagaactg atgattgcct gcgacactgc cgaacttgtc ggcatcttgt agccaaaaaa
     2941 gggacgagtc cgcacaatgc cgtctgaaac cccaaacgcc cgattaggct gatgaggatt
     3001 ttagacggca ttgttcattt ttttgttatc ttgcattttt gtgcggacgg tggaatttca
     3061 tcctgtaaac ataaatattt gtcggaaaac agaaaccctc cgccgccatt tctacgaaag
     3121 caggaaacca gcaacgcaaa gcgacaggga tttgttggaa atgaccgaaa ccgaacgaac
     3181 cggattcccg cctgcgcggg aatgacggga ttttctgttt ttgtggaaat gacgggattt
     3241 tgaatttcgg gcgtacaata cggaaaacat gacgataagg aaaaaaacta tggcacagtt
     3301 tttcgctatt catcccgaca acccccaaga acgcctcatc aagcaggcgg tcgaaatcgt
     3361 caataaaggc ggcgtggtcg tttatccgac cgattcctgt tatgccttgg gttgcaaact
     3421 cggcgataag gcggcgatgg aacgcatact ctccatccga aaaatcgatt tgaaacacca
     3481 cctgaccctg atgtgcgccg atttgagcga gttgggcaca tacgccaaaa tcgacaacgt
     3541 acagtttcgt cagcttaagg ccgccacgcc cgggccttat acctttatct tgcaggcgac
     3601 gaaggaagtg ccggcgcgca cgctgcaccc gaaacgcaaa accatcgggc tgcgtattcc
     3661 cgataatgcc attgcacaag ccctgctggg ggaattgggc gagccgcttt taagctgcac
     3721 cctgatgctg cccgaagacg gcgaaccatt gaccgatcct tatgaaatcc gcgagcgttt
     3781 ggaacacgcc gtcgatttgg tgattgacgg cggctggtgc ggaaccgagc cgaccaccgt
     3841 cgtcgatatg accgacggca cggaactggt gcgccggggt tgtggcgata cggcggtgtt
     3901 cggtttgtag ggaaaccgat gccgtctgaa gcatcggctg ttcagacggc attgcgcgcc
     3961 ttgccggcgg cagtccgaaa tgccggcgcg tatcgcgctc ggtcggaata tccgtttgaa
     4021 acggcatttt gatgcattac tgcaccgcaa tcggaattct cggttcgtag agcaggtcgt
     4081 aggtcggctt gttgagcagg tcttggagcg tgaaaccgtc cagatacgtg aaaaacgact
     4141 tcattgcacc gccgagtatg cctgtcagac ggcaggacgg tgtaatcagg cattcgttgt
     4201 tcgaacccat acattcgacc agctgcatcg gttcgaggtg gcggacgacc gagccgatgt
     4261 tgatgcggtc gggcggtgtg gcaagccgca gaccgccgcc ttttccgcgc acgctgtgga
     4321 ggaagccgcc tttgaccagc gcggtaacga ccttcatcag atggcttttg gaaatgccgt
     4381 aggttacggc gatggtactg atgttgacca gcgcatcgtc gttgatggca gtgtagataa
     4441 ggacgcgcag cccgtagtcc gtatgttgtg tcaaatacat gattttctcg gtatggattg
     4501 ttattcttat cggtacggtt taaggttcac ggacaatacc ttaatggttg aaaccctgtc
     4561 cgtcggggcg gtagaatgca gcctgtctgc ggcggtatgc cgtctgaaac atccgcgcta
     4621 ccgtttgaga atttgttatt gtaactcaaa atcatgaaac cgttgaaacg acatcccgcc
     4681 cttatcgggc tttcgcgtga ccaccaccat tcgctttccc tgtgcgtgcg tctgttgcgg
     4741 acgccggaag aaaggcatcg ggacgaactc gaaccgcatt tttccgaatt ggaaacccat
     4801 tttcgcgaag aagaaaccaa gtttgcccca atttggcaga atgtcgcccc cgaattgaaa
     4861 caacgtttcg agaaagacca cgcccgactg cggcagatga tggcaagccc cgaatacggt
     4921 aacgcggcgt ggaataccgc ttttgccaca accctgcgcg accacgcgcg ctttgaagaa
     4981 cgcgagctgt ttcccgccgc cgaaccgttt ttgccggcat gattccgttt tgcggcaaat
     5041 atattaatga taaacaagga acacacatga aatttaccaa gcaccccgtc tgggcaatgg
     5101 cgttccgccc gttttattcg ctggcggctc tgtacggcgc attgtccgta ttgctgtggg
     5161 gtttcggcta cacgggaacg cacgagctgt ccggtttcta ttggcacgcg catgagatga
     5221 tttggggtta tgccggactg gtcgtcgtcg ccttcctgct gaccgccgtc gccacttgga
     5281 cggggcagcc gcccacgcgg ggcggcgttc tggtcggctt gactatcttt tggctggctg
     5341 cgcggattgc cgcctttatc ccgggttggg gtgcgtcggc aagcggcata ctcggtacgc
     5401 tgtttttctg gtacggcgcg gtgtgcatgg ctttgcccgt tatccgttca cagaatcaac
     5461 gcaactatgt tgccgtgttc gcgctgttcg tcttgggcgg cacgcatgcg gcgttccacg
     5521 tccagctgca caacggcaac ctaggcggac tcttgagcgg attgcagtcg ggcttggtga
     5581 tggtgtcggg ttttatcggt ctgattggta cgcggattat ttcgtttttt acgtccaaac
     5641 gcttgaatgt gccgcagatt cccagtccga aatgggtggc gcaggcttcg ctgtggctgc
     5701 ccatgctgac tgccatgctg atggcgcacg gtgtgttggc ttggctgtct gccgtttttg
     5761 cctttgcggc aggtgtgatt tttaccgtgc aggtgtaccg ctggtggtat aaacccgtgt
     5821 tgaaagagcc gatgctgtgg attctgtttg ccggctatct gtttaccgga ttggggctga
     5881 ttgcggtcgg cgcgtcttat ttcaaacccg ctttcctcaa tctgggtgtg catctgatcg
     5941 gggtcggcgg tatcggcgtg ctgactttgg gcatgatggc gcgtaccgcg cttggtcata
     6001 cgggcaatcc gatttatccg ccgcccaaag ccgttcccgt tgcgttttgg ctgatgatgg
     6061 cggcaaccgc cgtccgtatg gttgccgtat tttcttccgg cactgcctac acgcacagca
     6121 tccgcacctc ttcggttttg tttgcactcg cgcttttggt gtatgcgtgg aagtatattc
     6181 cttggctgat tcgtccgcgt tcggacggca ggcccggttg agacaaaccg ccgcagattt
     6241 cgggtctggg cggtttgctt ttcagacggc agggcggtca gttgccgtcc agccagcggt
     6301 cgcgtgtggt tttggcttct tcaaaatagc ggtacagggc ttcgcggtcg tcggtggtca
     6361 ggatgtttgc caaaacgtcc aactgtttgc ccaagccttg aaccagttgc agcaggctgt
     6421 ctttgttggc aaggcagatg tccgcccaca cggcgggatg accggaggcg atgcgggtga
     6481 agtcccgaaa gcccgtggcg gcgaatttca gatattcctg tccgtcgggg tggtcgagaa
     6541 tctggtggac ataggcgaag gcggtcaggt ggggcatatg ggagacggcg gcgaaaaccg
     6601 cgtcgtggcg ttgcgcgtcc atcgtataaa tttccgcacc gaccgcgtgc cacaggtttt
     6661 ctaccaaggc aatgccgtct gaatgttcgc cgccgtgtgg cgtgatgatg agttttctgt
     6721 ggcggaacag cccgaactgc gcggcttgcg caccgcttct gtccgaaccg gcaatcgggt
     6781 gggcggcgat gcagcggtgc aggcggtcgg gcagacagcg gcggaaggct tcgatgaccg
     6841 aagatttggt gctgccgaca tcggaaatcc aagtgtgttc cggcaaaacg gggcgcagcg
     6901 cggtcaaaat ggcaggaacg gtggcgacgg gtgtggcaat cagtaccaag tccgcaccgc
     6961 cgatgctgtc cgcgtcgatg gcaacggaag cccggtcaat cacgccgcgt tccaatgcac
     7021 gttcgaggtt gtcgcggtcg gtgtcgatac cggtaacggt gcggacgagt ccctgccttt
     7081 tgaggtcgag aacgaacgaa ccgccgatca gccctacacc gatgagggca atatggttca
     7141 aaatgggcat ttgtgtaaac ggttttcgca aagtaccgtc atggtagcct atcggcggaa
     7201 tatgccgcaa ggtcggcagg aaaaaggaga agaaatggac aaaatcagag ttgccgccgt
     7261 gcagatggtg tcgggcgtgt cgccggaaac caacgtcgcc gccatgaaac gcctggtcgc
     7321 acgggcggcg gagcagggtg cggattgggt gctgctgccc gaatattggg tgctgatggg
     7381 cgcaaacgat accgacaaac tcgcgcttgc cgagcctttg ggcggcggac gctttcagac
     7441 ggcattgagc gaaacggcga aagaatgcgg cgtggtgctg ttcggcggaa ccgtgccgct
     7501 gcaaagcccc gaggcgggca aggtcatgaa tactttattg gtgtacgggc gcgacggcaa
     7561 acggacgggg ctgtaccaca aaatgcacct cttcggcttt tccggtttgg gcgaacgcta
     7621 tgccgaagcc gataccatca gcgcagggcg ggaagtgccg cacttgtcgg cagaaggcgt
     7681 gtcggtggcg gcgggcattt gttacgatgt ccgctttccc gaatttttcc gccgccagct
     7741 gccgtttgac gtattgatgc tgcccgctgc gtttacgcac acgacgggca aggcgcattg
     7801 ggagctgctg ctgcgcgcgc gtgccgtcga aaaccaatgt tacgtcgtgg cggcggcaca
     7861 gggcggtttg cacgaaaacg gacggcgcac gttcggacac agcatgattg tcgatccgtg
     7921 gggcgacgta ttggacgtat tacccgaagg cgaaggcatc gttacggcag acatcgatgc
     7981 caaccgcctg aacagcgtcc gcaaccgcct gcccgccttg aaataccggg ttttggatgc
     8041 cgtctgaagg ttcagacggc atcggtgccg gggaatcagc gggctgtagg atgggcttca
     8101 gcctgccatt tccatcaatc ccacatttct accgttttca tcgaatccat cgaatccgcc
     8161 cctttcgacg acccagtcct acgcaactga accgtcattc ccacggaagt gggaatctag
     8221 aatctcgggg gtttcagtca tttccgatag attcccgccg cgtcaggggt ctggattccc
     8281 gcctgcgcgg gaatgacgaa tccatccata cggaaacctg caccacgtca ttcccacgaa
     8341 agtgggaatc tagaacgcgg ggtttgagca actgttttta tccgataagt ttctgtgcgg
     8401 acaggtctag attcccgcct gcgcgggaat gacgggtttt aagattgcgg tattgtcagg
     8461 aatgacgaat ccatccatac ggaaacctgc accacgtcat tcccacggaa gtgggaatct
     8521 agaatctcgg ggtttcagtc atttccgata gattcccgcc gcgtcggggg tctggattcc
     8581 cgcctgcgcg ggaatgacga atttcgagat tacggtgttg tcggaacgaa actgaatcgt
     8641 cattcccacg aaagtgggaa tctagaacgc ggggtttggg caactgtttt tatccgataa
     8701 gtttctgtgc ggacaggtct ggattcccgc ctgcgcggga atggcgggtt tc
//
LOCUS       DAWFIT010000083         8598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFIT010000083 DAWFIT010000000
VERSION     DAWFIT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.083"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(113..631)
                     /locus_tag="WGQ85_001744"
     CDS             complement(113..631)
                     /locus_tag="WGQ85_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0751116.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(651..2036)
                     /locus_tag="WGQ85_001745"
     CDS             complement(651..2036)
                     /locus_tag="WGQ85_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0751117.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKFVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2275..3816
                     /locus_tag="WGQ85_001746"
     CDS             2275..3816
                     /locus_tag="WGQ85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0751118.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3845..4444
                     /locus_tag="WGQ85_001747"
     CDS             3845..4444
                     /locus_tag="WGQ85_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0751119.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4441..5046
                     /gene="nadD"
                     /locus_tag="WGQ85_001748"
     CDS             4441..5046
                     /gene="nadD"
                     /locus_tag="WGQ85_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0751120.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLYQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5105..5491
                     /gene="rsfS"
                     /locus_tag="WGQ85_001749"
     CDS             5105..5491
                     /gene="rsfS"
                     /locus_tag="WGQ85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0751121.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5545..6015
                     /gene="rlmH"
                     /locus_tag="WGQ85_001750"
     CDS             5545..6015
                     /gene="rlmH"
                     /locus_tag="WGQ85_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0751122.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6088..6354
                     /locus_tag="WGQ85_001751"
     CDS             6088..6354
                     /locus_tag="WGQ85_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0751123.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6511..7194
                     /locus_tag="WGQ85_001752"
     CDS             6511..7194
                     /locus_tag="WGQ85_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0751124.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7318..7830
                     /gene="coaD"
                     /locus_tag="WGQ85_001753"
     CDS             7318..7830
                     /gene="coaD"
                     /locus_tag="WGQ85_001753"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0751125.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7862..8515
                     /locus_tag="WGQ85_001754"
     CDS             7862..8515
                     /locus_tag="WGQ85_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0751126.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1871 a         2279 c         2509 g         1939 t
ORIGIN      
        1 aacgctgtac tggtttttgt taatccacta taaatcaatg taatccattc tgttcccgat
       61 gtttatgccg tctgaaaccc atcctgtgtc gggcttcaga cggcatattg cttcaaagca
      121 ggttttccga ggcaacccag ttcagaatat cggcttcgac cgcttcgggc gtgccggggt
      181 tggcgatttt gaccccgtat tcgccttcgc ccagttcctc gaggatttcc agttgcgaat
      241 cgagcatccc tgctttcatg taatgtcctt tgcgcgacat catgcgctcg aggttgatgt
      301 cttgcggcgg actgaggtgg atgaaggcag ctttgccttc ggctccgcgc agaatgtcgc
      361 ggtagccgcg tttgagggcg gaacaggtta cgatggtgtg gttcgcaccg ttttgcgcct
      421 gttgcgtcat ccagtcgcgc agattgccca accacggata gcggtcttca tcggtcagcg
      481 gaatacccgc gcccatcttg tcgcggttgg cttgggtgtg gaactcgtcg ccttcggcat
      541 agggacattg accgaggtgt ttctgcaggg acagcgcggc ggtggtcttg ccgcagccgc
      601 atacgcccat aacgacaaaa tgcgtagtca tttactatcc tttccgtctg tcagacgatg
      661 gcgaacagca gtgcggacaa ggcaaagccg atgagtgcga tgagggtttg gttgaccgtc
      721 caggttttca gcgtggtcgg tacgtccatg tccaagagac ggccgaccag ccagaagccg
      781 gagtcgttga agtggctgca accgaccgaa cctgccgccg ttgccaatac gatacaggcg
      841 agctgccagt cggtaaagcc ggcggcggca acggcaggag ccatcagcgc ggcggcggtg
      901 gtcagggcga cggttgccga accttgcgcg atacgcagtg ccaaggcgac aaggaaacag
      961 cccaaaagga cgggaatgcc caaatccgcc atgctgtcgg cgagtgcctt gccgatgccg
     1021 gaagcgcgca aaacgccgcc gaacataccg cccgcgccgg taatcagaat cacggaacag
     1081 acgggggcga gtgcgccgtc cacggttttt tccaacgcgc tgccgctttc gccgcgtttg
     1141 cgtcccaaga caaacagtgc gaccaatacg gaaatcagaa gggcgatcgg tgtcgaaccg
     1201 attatttttg ccgtctgaac ccaggtttcg tccgcactta cgaatttttc gctgatgagg
     1261 gccgatacgc cggtattcag gaaaatcagc agcatgggaa tcagcatgat ggcgacgacc
     1321 gttcctgctt tggcaggttc tttcggcagg tcgttgtctt gcgtgccgcc gctgagcagt
     1381 tcgggaacgg gaacatggat ggtgcgcccc aacactttgc cgagcatata gccgctgaaa
     1441 taccatgtga tgaaggcggt cggcagaccc aaaatcaaaa cttggccgat gttcgcgccg
     1501 taaaattcgg aagcggcaat cgggcccgga tggggcggca ggaagacgtg catgacggaa
     1561 aatgcgccga tggaggcaag cgcgaagggc agtacgtcct gtttcatgcg ccgtgcggtg
     1621 gcgaacacga tgggcagcat gacgattagt ccggcatcga agaaaatcgg gaagccgaaa
     1681 atcagcgagg caacgcccag cgcgaacggt gcgcgttttt cgccgaacat ccggatcagc
     1741 gcgtccgcca gcgactgtgc gccgccggat gtttcgacca gacgtccgag catcgcgccc
     1801 aggccgacca gaagcgccac gccgccgagc gtgccgccga agtttttgac cagtacgtcg
     1861 ttgacaatgc tgcctgtggg caaaccggtt gccaaagccg tcagcaggct gacgatgacc
     1921 agtgtcagca gcgcgtggat gcggaatttg acgattaaaa tcagaatgag gatgattgcc
     1981 gccgccgaaa tgcccaacag ggtttgcgcg gacagcgtct gtgtccagcc gtccatttgt
     2041 aaagcctttc tctgtaataa aagtacaaaa ttgtcaggtt tttttaagtt gtgcgaaaac
     2101 gatagcacaa atcgggcggt aagttgtttg tctgaagtta tattcctgtt tatttgaacg
     2161 atttatttca gacggcggga ggaaggcgga tacggtatgt agtcggattg aaatatacgg
     2221 tatgtggggc ttttgggtac aatcgcaaca tcagtgtttc aagtggggaa ggcgatggat
     2281 ggacggcgtt gggcggtatg gggtgctttt gccctgctgc cttcggcttt tttggcggta
     2341 atggtcgttg cgcctttgtg ggcggtggcg gcgtatgacg gtttggcgtg gcgcgcggtg
     2401 ctgtcggatg cctatatgct caaacgtttg gcgtggacgg tatttcaggc agcggcaacc
     2461 tgtgtgctgg tgctgccttt gggcgtgcct gtcgcgtggg tgctggcgcg gctggcgttt
     2521 ccggggcggg ctttggtgct gcgcctgctg atgctgcctt ttgtgatgcc cacgttggtg
     2581 gcgggcgtgg gcgtgctggc tctgttcggg gcggacggcc tgttgtggcg cggacggcag
     2641 gatacgccgt atctgttgtt gtacggcaat gtgtttttca accttcctgt gttggtcagg
     2701 gcggcgtatc aggggtttgt gcaagtgcct gcggcacggc ttcagacggc acggacgttg
     2761 ggcgcggggg cgtggcggcg gtttttggac attgaaatgc ccgttttgcg cccgtggctt
     2821 gccggcggcg tgtgccttgt cttcctgtat tgtttttcgg ggttcgggct ggcattgctg
     2881 ctgggcggca gccgttatgc cacggtcgaa gtggaaattt accagttggt catgttcgaa
     2941 ctcgatatgg cggttgcttc ggtgctggtg tggctggtgt tgggggtaac ggcggcggca
     3001 gggttgctgt atgcgtggtt cggcaggcgc gcggtttcgg ataaggcggt ttcccctgtg
     3061 atgccgtcgc cgccgcagtc ggtcggggaa tatgtgctgc tggcgtttgc ggcggcggtg
     3121 ttgtctgtgt gctgcctgtt tcctttgttg gcaattgttg tgaaagcgtg gtcggccggc
     3181 gaatcgtggc gtgtgttaat ggaaagtgaa acgtggcagg cggtgtggaa tactttgcgc
     3241 ttctcggcgg cggcggtgta tgcggcggcg gttttgggtg tggtgtatgc ggcggcggcg
     3301 cggcggtcgg cgtggatgcg cgggctgatg tttttgccgt ttatggtgtc gccggtttgt
     3361 gtttcggcgg gcgtgctgct gctttatccg cagtggacgg cttcgttgcc gctgctgctg
     3421 gcgatgtatg cgctgctggc gtatccgttt gtggcaaaag atgttttatc agcctgggat
     3481 gcactgccgc cggattacgg cagggcggcg gcgggtttgg gtgcaaacgg ctttcagacg
     3541 gcatgccgca tcacgttccc cctcttgaaa ccggcgttgc ggcgcggtct gactttggcg
     3601 gcggcaacct gcgtgggcga atttgcggca accttgttcc tgtcgcgtcc cgagtggcag
     3661 acgctgacga ctttgattta tgcctatttg ggacgcgcgg gtgaggataa ttacgcgcgg
     3721 gcgatggtgc tgacattgct gttggcggcg ttcgcggtgt gcattttcct gctgttggac
     3781 ggcggcgaag gcggaaaacg gacggaaacg ttataatgcg aacccttttt cagaggacgg
     3841 caaaatgagc gagttggata acatccttgc ccacaaccgg cagtttgtcg aatcgggcga
     3901 atatgaaaaa tactttaccg acaaataccc cgaacgcggg ctggcggttt tgtcctgtat
     3961 ggatgcgcgg attatcgggc tgctgcccga cgcgttgggt ttgaaaaacg gcgatgccaa
     4021 gctgattaaa aatgccggcg cgctggttac gcacccgtgg ggttcggtga tgcggagcct
     4081 tttggttgcc gtgtttgaat tgaaggtcag agagattatg gtcatcgccc atcacgattg
     4141 cggtatgcag gggctgaatg ccgaagaatt cctcgggcgc gtccgggaaa gccggattcc
     4201 cgaagaccgt atcgaaaccc tgcgttatgc cggtatcgac ctcgacggct ggctgaccgg
     4261 tttcgacaac gtcgaagaca gcgtgcgcca cacggtggac cttatccgta accatccgct
     4321 gatgccgcgc catattgccg ttcacggatt ggtcatccat cccgttaccg gcaaactgac
     4381 gctggttgtg gacggcagtg tttcagacgg catggactta tcggaaggaa tggaaacatc
     4441 atgaagaaaa tcggactgtt cggcggcact ttcgacccga tacacaacgg gcatctccac
     4501 atcgcccgcg cctttgccga cgaaatcggc ttggacgcgg ttgttttcct gccgacaggc
     4561 ggcccgtatc acaaagacgc agcctccgct tccgccgccg accgccttgc catggtcgaa
     4621 ctggcgacgg cagaagacgc gcgttttgcc gtcagcgatt gcgacatcgt ccgagaaggt
     4681 gcaacctata cttttgatac cgtccaaatc ttccgccagc agttcccatc cgcgcaactc
     4741 tggtggctga tgggcagcga cagcctgatg aagctgcaca catggaaaaa atggcagatg
     4801 ctcgtgcgcg aaaccaatat cgccgtcgcc atgcggcagg gcgacagtct gtaccaaacc
     4861 ccgcgcgaac tgcacgcgtg gctgggcaag tcccttcagg acggcagcgt ccgcatcttg
     4921 tccgccccga tgcataatgt gtcgtcaacg gaaatccgcc gcaaccttgc cggccaaggc
     4981 gtttcagacg gcatcccgcc tgccgccgca cgctacatcc gcgaacacgg tttgtatgaa
     5041 aaataaagtc aaatcagtaa gaaacggcta taatgccgtc tgaaaacatc ccgttaggaa
     5101 cataatgaac gaacaagaac tgcaagacct gcaaaaaatg gtcggggtcg ccgtcaacgc
     5161 cctcgaagac atcaaagcca aagacatttc cgttctcgaa acgcaagaca aaacttcgct
     5221 gtttgccaga atgattatcg ccagcggcga cagtacgcgc caagtcaaag cactggccaa
     5281 caacgttgcc gtcgatttga aagaagccgg ttttgaaatc ctcagcaccg aaggcgacag
     5341 cggcgaatgg acgctcgttg atgcaggaga cctcgtcgtc cacgttatgc tccccgccgt
     5401 gcgcgacttc tatgatatcg acaccctttg gggcggcgag aaaccgagtt ttcacgccgg
     5461 aatgcagaag ccgtggcacg cggcagactg attcccgatg ccgtctgaat gttcagacgg
     5521 cattttcctg taagggagaa agcattgaac atcaccgttt tggcagtcgg caccaaaatg
     5581 ccgcgctggg ttgatgaggc cgtcgccgaa tacgccaaac gcttcggacg cgacgtcgcc
     5641 tacgcactca aagaaatcaa acccgaaaaa cgcggcgcgg gcgtgaatgc cgcccaaggt
     5701 atggcggcgg aagaaaaacg catccttgaa gccattccgc aaggcgcgtt cctcgtcgtt
     5761 cttgacgaac gcggcaaagc accgacctcc gtcgagctgg cggaacacct caaaagctgg
     5821 cagcaaaacg gcgaacacgt ctgcttcgtc atcggcggcg cggacggtat gaccgaccgc
     5881 ctcaaacagc aggcacgcat gatgatgcgc ctgtccagcc tgaccctgcc gcacgggatg
     5941 gtgcgcgtcc ttctgaccga gcagctctac cgcgccgcct ccatcctgca caaccatccc
     6001 tatcatcggg aataagaagg cttttgtccg tatccctaat cggttaaaat caccccgtta
     6061 tttctgaaca cagcaaagga atccgctatg gcacgaatgg tattctgcgt caagctcaac
     6121 aaagaagccg aaggcatgaa atttccaccg cttcccaacg aattgggcaa gcgcattttt
     6181 gaaaacgtat cgcaagaagc atggatggcg tggacgcgcc accaaacgat gctgattaac
     6241 gaaaaccgct taagccttgc cgatccgcgc gcgcgcgaat acctagctca gcagatggag
     6301 cagtatttct tcggcgacgg cgcggatgcc gtgcagggat acgttccgca ataacggttt
     6361 tccgttttga acacaggctg tccgaacctg cttcagacgg cctttaaaat acgccggaca
     6421 actttatctt cacgcaaccg gacaaatttt gacattgggc aatgttaaaa tcccccatta
     6481 ttcccaaccg cgctttcagg agcagatgat atgcaacacg acgtttacga ctacaccgcg
     6541 catacggttt ctaaaaacac cgtcctgcag aaaacctacc gcctgctcgg attttcattc
     6601 attccggctg ccgcaggcgc ggcacttgcc gccaatgccg gtttcaattt ttacgccgcc
     6661 ttcggttcgc gctggatagg gtttgccgtc gtgttggcgt ttttctacgg tatgatccac
     6721 ttcatcgaga aaaaccgtta cagcaatacc ggcgttaccc tgctgatggt attcacattc
     6781 ggtatgggcg tattgatcgg ccccgtgctg caatacgcgc tccatattgc cgacggtgcg
     6841 aaaatcgtcg gcattgccgc cgcgatgacc gccgccgtct ttttaacgat gtccgccttg
     6901 gcgcgccgaa cccggctcga tatgaacgcg ctcggacgct tcctgaccgt aggtgcggtc
     6961 attctgatgg tcgccgtggt tgccaatctg tttttgggta ttcccgcact cgccctgacc
     7021 atttccgccg gttttgtctt gttcagttcc ttaatgatta tgtggcaggt acgcaccgtc
     7081 atcgacggcg gcgaagacag ccatatcagc gcggcactga cactgtttat ctcgctttac
     7141 aacatcttca gcagcctgct gaacatcctg ttgtctttaa acggcgagga ttgatgccgg
     7201 taaaacgccg tccgactatg ccgtctgaaa atgcttcaga cggcatttta ctttgggcat
     7261 acaatacgga tgcacacgca ccaacacaca gccgactatg aagccgggag gaatcaaatg
     7321 ttaccgaata caccgcgccg cgccgtttat gccggcagtt tcgatccgcc cacattgggg
     7381 catctgtgga tgatacggca ggcgcaatct atgtttgacg aactcatcgt cgccatcggc
     7441 attaaccccg acaaacgcag cacctatacc gtcgctgaaa ggcaggatat gttgtgcgat
     7501 attactaaaa tgtttcccaa cgtcagaacc gatgtatttg aaaaccgatt tctggtgcat
     7561 tatgcccgtg aggtagatgc aggattcatc gtgcgcggca tccgttctgc ttcggattac
     7621 gaatacgaac gttccatgcg ccacatcaac agcgacctcg cccccgaaat atccaccgta
     7681 ttcctcatgc cgccgcgcga aatcgccgaa gtgtcgtcca ctatggtcaa aggactggtc
     7741 gggccggaag gctggacgga aacgatccac cgctacgtgc cgcaagctgt gtacgaaaaa
     7801 atccttgccg aacatcaaca cgaaaattga acccttctat ttttgcaagg catatatgga
     7861 tatgttggaa attttgttcc gccatcaaga cttcgtcgcc atcaacaaac cgggcggcat
     7921 atccgtccac caagacagcg gcgagaccgg actggcccga acactcgcca tacagttggg
     7981 cgtagagcgt gtgtggctgc tgcaccgatt ggacaaacag accagcggca ttttactgtt
     8041 tgccctcaac aaagaaagcg cgtccgccct tagcggacag tttgccggca aaagcatcaa
     8101 aaaaacctat ttggcactgt ccgaccgcaa gccgtccaaa aagcagggtt ggattaaagg
     8161 cggaatggaa aaatccagat gtggaatgtg gaagttgacg cgcaatacag aaaatatcgc
     8221 cgttacccga ttccacagca tcagtatcgc cgaaaaactg cggctgttca tcctggaacc
     8281 gcatacgggc aaaacgcacc aattgagagt ggcgatgaaa agtttgggca gtccgattct
     8341 gggcgacagc ttatacggcg gaacagaatc cgaaaccatg tttctgtatg catggaaaat
     8401 acaatttgcc taccaaaacc gacaaatcga aattgttgca cccttaaaaa acgaatggca
     8461 gactgaaaat atctctcatg cgctggaaga attttatatg gcaaaagcag gttaataaat
     8521 ataaattaat ttataaacaa tatgataaat atttttataa aaatttttca agaaaacggt
     8581 tgactgcaaa tctcttca
//
LOCUS       DAWFIT010000084         8367 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFIT010000084 DAWFIT010000000
VERSION     DAWFIT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.084"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(329..604)
                     /locus_tag="WGQ85_001755"
     CDS             complement(329..604)
                     /locus_tag="WGQ85_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0751127.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(601..1884)
                     /locus_tag="WGQ85_001756"
     CDS             complement(601..1884)
                     /locus_tag="WGQ85_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0751128.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGQ85_001757"
     CDS             complement(2040..3458)
                     /gene="glnA"
                     /locus_tag="WGQ85_001757"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0751129.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3542..3691
                     /locus_tag="WGQ85_001758"
     CDS             3542..3691
                     /locus_tag="WGQ85_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751130.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            3769..4578
                     /gene="aroE"
                     /locus_tag="WGQ85_001759"
     CDS             3769..4578
                     /gene="aroE"
                     /locus_tag="WGQ85_001759"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0751131.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4580..5281
                     /gene="mtgA"
                     /locus_tag="WGQ85_001760"
     CDS             4580..5281
                     /gene="mtgA"
                     /locus_tag="WGQ85_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0751132.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5606..5681)
                     /locus_tag="WGQ85_001761"
     tRNA            complement(5606..5681)
                     /locus_tag="WGQ85_001761"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5646..5648),aa:Phe,seq:gaa)
     gene            complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGQ85_001762"
     CDS             complement(5872..6606)
                     /gene="lptB"
                     /locus_tag="WGQ85_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0751133.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGQ85_001763"
     CDS             complement(6680..7210)
                     /gene="lptA"
                     /locus_tag="WGQ85_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0751134.1"
                     /translation="MIQKICKLFVLIAFFSVSPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGQ85_001764"
     CDS             complement(7191..7772)
                     /gene="lptC"
                     /locus_tag="WGQ85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0751135.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7769..8305)
                     /locus_tag="WGQ85_001765"
     CDS             complement(7769..8305)
                     /locus_tag="WGQ85_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0751136.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1934 a         2266 c         2178 g         1989 t
ORIGIN      
        1 gttgacatat tgtgcgatat cgagggctgt accgtatttt cccatcgtat tttcccgttc
       61 tttatatgag cggcaggcat cctcataaaa gaagggatac cgataaggaa aaataccccg
      121 gcataaaaaa tatactgatt aatcaataca aatccaacaa acagttcatc gtattctttt
      181 ctgttggcgt ataaaaaata ctgatatatg ccttcacttt catagggcga cgctttatgt
      241 accgtcctgt gtgttgaaac gtcggttttc cttaccgcct gccccgcttt tccctgattg
      301 ctgccaactg ccgtccgaac ctgaaccgtc aggtttcaga cggcattttt tctgcctgct
      361 cccacaattg ccgtttttgt gcggcggtca gttttttgac ggcgatttcc tgcctgagtg
      421 ccgttccttt atccatcccg cctgcaacga tacgcatcgc caccggtttg aatacgcggg
      481 tatatttcgc gcctttaccg gttgtgtggg cggcaagccg ctgttgcgga ttcgggctga
      541 tgccgcaata gaacgcgctg ttttcacaca atatcaggta aacgctccag tttgacgcgt
      601 tcatctgcct gcatcctgag ttttctcccc gttccaaggc gcaactttca gcagcagcag
      661 catacccggt agggcgagtg cgaaacacag ctggaaaaac ggtacatacc cgagccattc
      721 gatcagataa ccggcaaagg aattgatgac cgtacgggga acagcggaca ggctggtaaa
      781 cagcgcaagc tgcgttgcgg taaatgcggg attggtttca cgcgccatat acgatacgaa
      841 cgccgccgtc cccaaaccca cgccgactgc ctctgcgccg ataactgccg ccagcatcag
      901 cctctcgcct gtgccgaccg tgtcgaaatg tccgaaccct gccagccata caaaccccaa
      961 aacggttaca gcctgcaccg cgccaaatat ccacaaggct ttgtttacgc cgattttcag
     1021 catccacacg ccgcccaaga tacctgccgc cactgccggc cacagtcctg catttttcgc
     1081 aatcaaaccg atgtctgtct tgctgaaacc catatccaga taaaacggcg ttgccaacgc
     1141 ggttgccata ctgtcgccga gtttgtaaag gaagataaac agcagcacgc acaccgccga
     1201 agcgatgccc ttgcgcgtaa aaaattcttt aaacggctct accacggtct gcttcaacgt
     1261 tttaggaacg gcaggcggca acacgggttc gcgcgcaaga aacagcgtca tcagaaggcc
     1321 gggcagcata aacagcgaag tgatgacaaa cacctccggc cacggcatcc tgtctgccaa
     1381 caccaaactc aatgaaccgg gaatcagggc ggcaatccgg taggcgttca catgaaccga
     1441 gttgcccaaa cccaattctt cgtctgacaa aatctcgcgc ctgaacgcat ccaatacaat
     1501 atcctgactg gcggaaaaaa aagcgacaag caccgacaag ccggcaatca gcggcagatg
     1561 attacggggg tttaaaaagg cataggccgc caaagccgcc agcaaccctg cctgcgtcag
     1621 cagcatccac ccgcgccgcc gtcccaaaac gggcagcctg accgcgtcca tcagcggcga
     1681 ccacaaaaat ttccaagtaa acggcagacc gattaacgcc atcagcccga tgctcttcaa
     1741 atccacctgc tcgctgcgca accacgccgg aatcaggtta atcagaaagt acagcggcag
     1801 ccccgaggta aaccccgtaa aaatacagac aagcatattt cgggaaaaga tttgcccgat
     1861 aaaacctgat ttcgatgcag tcatcgttcg tgcagcccaa aaaaaacaaa agcctattgt
     1921 agcaaatatc gcccgcctgc tgaaaaatgc cgccccgcgc cgccggtttg ccgttcaaat
     1981 gcaaaatgcc gtctgaaact gtgttcagac ggcattgctt ttctgaaaac cagacgtgct
     2041 tacaggctgt aatacatttc aaattccagc gggtgcggcg ccatgcggat gcggcgtacg
     2101 tcttcttctt tgaaagcgat gtagctgtcg atccagtctt tgctgaacac gccgccgcgc
     2161 aggaggaatt cgtggtcggc tttgagggcg gccagtgctt cttccaaaga agcgcaaacg
     2221 gtcggcacca atgcatcttc ttccggcggc agatcgtaca ggtttttatc ggcagggtcg
     2281 cccggatgga ttttgttttg aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc
     2341 aaatacgggt tggcggtcgg atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg
     2401 ttcacggacg gaatgcggat ggaagcggaa cggtttttgg cggagtatgc cagtttggtc
     2461 ggcgcttcaa agtgcggcac gaggcgtttg taggagttgg tggacggatt ggtaatcgcg
     2521 ttcaaggctt tggcgtgttt gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag
     2581 ccggcatagc cgtcgcctgc gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg
     2641 tgcataccgc tgccgttgtc gcccataatc ggtttgggca taaaggtggc tgttttgccg
     2701 aagttgtggg caacgttttg aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg
     2761 accaaggtgg cgaaacgcgt gccgatttcc atttggctac cggtaccgac ttcactgtgg
     2821 tggacttcga cttcgatgcc gagttcttcc aaaatgttta ccatcgcgga gcgcaggtct
     2881 tgtcccgcgt caatcggagc aacgggcgcg taaccgcctt tgacggcagg gcggtggccg
     2941 gtgttttgac cgtccatatg cagaccgctt gcccacgcgc cgctttcgga cgtgatttcg
     3001 taacgggttt tgtgcatatc ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt
     3061 tcgggaccga aataggcggt gtcgccgatg ccggaagatt tcaaataggc ttcggctcgg
     3121 cgggcgatgg agcgcgggtc gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg
     3181 caagtcaaca caacagtcgc atcatcataa aaaggatcga cgaaggctgt agacgcatcg
     3241 gggcgcaact gcatatcgga agcctgaatg cctttccagc cgccgataga cgaaccgtca
     3301 aacgcctgac cgttttcaaa ccactcttcg gggtcttcca acacgatgcg cgcaggcacg
     3361 gtaaagtggt gctgcttgcc tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg
     3421 ctttcttcaa tcaattttac ggcgttttta atagacattt ttcagctcct ggaaaaggga
     3481 aatagcaaaa tatgcggaac gcggcggctt gacgcaaagt cggcatgatt ctaccacgtc
     3541 catgccgtct gaaactcgcc ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg
     3601 caagattgtc aacaatatgc ttctgtttgt ctgttcaaaa gcggaatacc cgcccttttg
     3661 tccgaatgcc gacaaaacag atgccgtctg aaagcagttt agacggcatc gttcccatct
     3721 tcgcgccgcc gttataaaat gaccgccccg ccttgatgga atatgactat gcacgccctc
     3781 ccccgctacg ccgtttttgg caaccccgtc gcccacagca agtcgccgca gattcatcaa
     3841 caatttgccc ttcaggaagg cgttgacatt gaatacgaac gcatttgcgc cgacatcggc
     3901 ggtttcgcgc aggcggtttc gacatttttt gaaacaggcg gttgcggggc aaacgttacc
     3961 gtaccgttca agcaggaagc gtttcatctg gcggacgagc attctgaacg cgcattggct
     4021 gccggcgcgg tcaacacgct gatttttctg aaaaacggaa aactgcgcgg cgacaatacc
     4081 gacggtatcg gtttggccaa cgacatcacg caggtcaaaa acattgccat cgaaggcaaa
     4141 accatcttgc ttttgggcgc gggcggcgcg gtgcgcggcg tgattcctgt tttgaaagaa
     4201 caccgtcctg cccgtatcgt cattgccaac cgcacccacg ccaaagccga agaattggcg
     4261 cggcttttcg gcattgaagc cgtcccgatg gcggatgtga acggcggttt tgatatcatc
     4321 atcaacggca cgtccggcgg cttgagcggt cagcttcctg ccgtcagtcc tgaaattttc
     4381 ctcggctgcc gccttgccta cgatatggtt tacggcgacg cggcgcagga gtttttgaac
     4441 tttgcccaaa gcaacggtgc ggccgaagtt tcagacggac tgggtatgct ggtcggtcaa
     4501 gcggcggctt cctacgccct ctggcgcgga tttacgcccg atatccgccc tgttatcgaa
     4561 tacatgaaag ccatgtaata tgttccgcat cgtcaaatgg ctgattgccc tgcccgtcgg
     4621 catctttatc tttttcaatg cctatgtgta cggcaacatc attacctacc gcgccgtcgc
     4681 gccccatcag actgccttta tgtcgatgcg gatgaagcag tttgaacagg aaggtcgcga
     4741 tgtcgcactg gattaccgct ggatgcccta caaccgcatc tcggtcaacc tgaaaaaagc
     4801 cctgattgct tccgaagatg cccgtttcgc cgggcacggc ggcttcgatt ggggcggcat
     4861 tcaaaacgcc atcaggcgca accggaacag cggcaaagtg aaggcgggcg gctcgaccat
     4921 cagccagcag cttgccaaaa acctcttcct caacgaaagc cgcagctata tccgcaaagg
     4981 cgaagaagcg gcgattaccg cgatgatgga agccgttacc gacaaagaca ggatttttga
     5041 actgtattta aactcaatcg aatggcacta cggcgttttc ggcgcggaag ccgcgtcccg
     5101 gtatttttat caaatacccg ccgccaagct gaccaaacag caggcggcaa aactgacggc
     5161 gcgcgtcccc gccccgctct actacgccga ccatccgaaa agcaaacggc tccgcaacaa
     5221 aaccaatatc gtgctcagac gcatgggttc ggcagagttg cctgaaagcg acacggactg
     5281 attgtttcag atatggaaat gccgtctgaa ctgcggttcg gacggcatat gttttctggt
     5341 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     5401 cacggaatcg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     5461 cgagccaacg ctgtaccggt ttaaatttaa tccactatac ctgcccgaca gccgcaaaga
     5521 ccgtatcata catagcaggc attgcggtgc gggattactg ccgcaaacgg caggcaaaaa
     5581 aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg gaatcgaacc gccgacacac
     5641 ggattttcaa tccgttgctc taccaactga gctatctggc ctttgtctgt ctcgttaaga
     5701 gatgcgtatt aaaccaaatt acgggacgac atgcaagcat atcgggcaat attctttgaa
     5761 aacaggttta agtcaatgtt ttaaatttga attaatttat gtaaaaacaa cataaatccg
     5821 tatttttgcc gcctgcccga cgatattagg tcgcccgtct gaaaaatatt ttcaatattt
     5881 gaagttctta cccagataaa cagaacgaac ctgttcgttt ccgaccaaat catcaggttt
     5941 tcccgatgcc agcaccgcac cgtctgaaat gatgtaggcg cggtcgcaga tgctgagggt
     6001 ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg cgcgacttga gaaatccgat
     6061 gattttttgg atgtcgatga cggcgatggg atcaacaccg gcaaaaggct catccagcaa
     6121 aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg acgcgccgcc gttcgccgcc
     6181 ggacagcgac ggcgcggggc tgcggcgcaa gtgtccgata ttaaggtcgg cgagcagttt
     6241 ttcgatttcc ctgtcgattt gatttttatc tttggttctg atttccaaga tggcgcggat
     6301 gttttgttcg acggtcattt tgcggaatat cgaggcttcc tgcggcaggt agccgacacc
     6361 gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct tgtccgtcta gggttacgct
     6421 gcctgcgtcg gcggcaatga gtccgactat catgtagaag ctggtggttt tacccgcgcc
     6481 gttgggcccg agcaggccga cgacttctcc gctttcgatt tcgagggaga agcttttaac
     6541 gacttggcgt tttttgaaac ttttttgcag gttttgaaca acaaggcggc tgacgtttgc
     6601 gctcataaat atctcttcaa tatcttcggt cggcaaatgc cgtccgaacc aggtttatgt
     6661 ttcagacggc attttgggat tattcggatt tttgcgtact cgaaggctgg ataacgacgc
     6721 tgaccctgcc ggatttggaa gcggattttg cgccggattt tgtgctgccg ctgatggtat
     6781 agacttcggt tttggtgttg tatgtaatca ccgcaccttc ggcgacatcg ccgccgcgct
     6841 gtactttggc attaccggtt aagactacgg tgctgcctgc agatgaataa gcaacgttgt
     6901 ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt ctggctgaag cggactggcg
     6961 aaccttccgc cctcacggat tcgccgcctt tgccgccgcg tgtaacattg acgcgggcgg
     7021 cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc gctgaatgtg gtgctttggt
     7081 tggcttgatc gagcgaacct tggtcggcct caatctgaat aggctgcctg ctgtcgcttt
     7141 gaagggcaaa agcgggggac accgaaaaaa atgctattaa aacaaatagc ttacatatct
     7201 tttgtatcat aaatcgtggc tttcacttta gatgagaagt tcaacatgcc tgttttgtgg
     7261 tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat attggaaact gacaggcgta
     7321 tcggtttggg catattgaga ttcggtatcg acgtgcagct tttcggcttc aactttaccc
     7381 gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt tgtttttaaa aagaacctgt
     7441 ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt cgtacaacaa cctgccttct
     7501 tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc tgctttcggg aaactgtttc
     7561 gcgcccttcg aactcaaatg ttctttcaag tatccctgtt cgtcaaaccg cctgccatcc
     7621 aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc tgacttcttc aatctcgact
     7681 tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg caaccgccaa tatcaatggg
     7741 aacgcaattc cgtaccgcca tcttactttc atttgatgta ctcgttcaaa gccgcgccca
     7801 aagtcccttg cgcctgcata atcaggtcgc acacttcgcg caccgcgccc gcaccgccgg
     7861 ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca atgcgcgtcg gggacggcaa
     7921 ccggcaatcc gcagcgtacc ataacgggca aatcgaccac gtcgtcgccg acaaaggcac
     7981 actcggcttc ttccacgccg acctgagcgc gcagctcttc atacgcggcg cgtttgtccg
     8041 aaatgccttt gaagtagtaa tttatgccca actgtttgac gcggatgccg acggagggcg
     8101 cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc ctgaagcatt ttcagaccgt
     8161 gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt atcgcggata aagatgcgcc
     8221 cgtcggtcaa aacgccgtcc acatccagga tcagcagttt gattttggcg gcgcgcgcct
     8281 gtaattcggg agaaattgcc tgcatgggat ttccttgttt tcagacggca tatcttacca
     8341 caaatgccgt ctgaaacggc tgcaacg
//
LOCUS       DAWFIT010000085         8345 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFIT010000085 DAWFIT010000000
VERSION     DAWFIT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.085"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(116..1219)
                     /locus_tag="WGQ85_001766"
     CDS             complement(116..1219)
                     /locus_tag="WGQ85_001766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0751137.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGQ85_001767"
     CDS             complement(1454..3010)
                     /gene="dnaA"
                     /locus_tag="WGQ85_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0751138.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPNEPRQAAGSASRPELEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDMDLARTALQDIIAEKHKVITADLIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3303..3437
                     /gene="rpmH"
                     /locus_tag="WGQ85_001768"
     CDS             3303..3437
                     /gene="rpmH"
                     /locus_tag="WGQ85_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0751139.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3440..3805
                     /locus_tag="WGQ85_001769"
     CDS             3440..3805
                     /locus_tag="WGQ85_001769"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0751140.1"
                     /translation="MDYRFGKQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRPTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3870..4091
                     /gene="yidD"
                     /locus_tag="WGQ85_001770"
     CDS             3870..4091
                     /gene="yidD"
                     /locus_tag="WGQ85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0751141.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4263..5900
                     /gene="yidC"
                     /locus_tag="WGQ85_001771"
     CDS             4263..5900
                     /gene="yidC"
                     /locus_tag="WGQ85_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0751142.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5981..6694)
                     /locus_tag="WGQ85_001772"
     CDS             complement(5981..6694)
                     /locus_tag="WGQ85_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0751143.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCVAADLTLPTQEIISRTIAQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGQ85_001773"
     CDS             complement(6753..7343)
                     /gene="maf"
                     /locus_tag="WGQ85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0751144.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7491..7994
                     /locus_tag="WGQ85_001774"
     CDS             7491..7994
                     /locus_tag="WGQ85_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0751145.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8028..8207
                     /gene="rpmF"
                     /locus_tag="WGQ85_001775"
     CDS             8028..8207
                     /gene="rpmF"
                     /locus_tag="WGQ85_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0751146.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         2059 a         2482 c         1989 g         1815 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatac ctttccgaca gccgaacaaa accccgaatc
       61 cgtctgcacg gttcggggca tatctccaat acgggcatcg tgctccggaa aaccgtcaaa
      121 ttcgcatcgg catcacgata tatttgaaat tcggattgtt cggcacggta aacagcgtcg
      181 agcggttggc atcgccgaag gcaagctgca tatcgtcgga atggatgttg cgcaacacgt
      241 ccatcagata gccgatattg aaaccgactt cgagttcgcc gccctgatag gcaatctcga
      301 tttcttcgcg cgcttcttcc tgctcgttgt tgctgcacac gacgctcaac aggccggatt
      361 gcaggaacag gcgcgcgcca cggaattttt cgttggcaag aatggacaca cgttccagcg
      421 cgcccaaaag ttcggcacgg gacaaaacga aaatcttgtc gttgtccaaa ggaatcacgc
      481 ggttgaaatc ggggaatttg ccgtcgatga ccttgctgac gatggtcgtg ccgttgcatt
      541 ggaaacgcac ctgtttgtcc agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca
      601 gtttgaacag ttccagcacc gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg
      661 catcaatcgc gcaggctgca taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact
      721 ggctgccctc aacctgcatc agcagaccgt tgagataata gcggatgtcc tgcaccgcca
      781 tgctgtactg cacttgcgac agcatggttt tgaaacgctc ctgctccagc gagaaagtcg
      841 cgctgatgtc ctcgccgaca ttcatcatcg gaaaatcggc ggcaggcagg gtctgcaggg
      901 caaaacgcga tttgcccgcc ttcagcgtca gacggctgtc gtcccaatcc agcgacacca
      961 gcgcaccggc aggcagtgcc cgtaaaatgt cctgaaattt cttggcattg gtggtgatgc
     1021 ggaagtcgtc cgcgccgccc tcgggacccg cagtgtcgat ttggatttcc aaatcggttg
     1081 ccaagagttt ggtctgaccg ccttttccct caatcaggac gttggacagg atgggcaggg
     1141 tgtggcggcg ttcgacgatg ccggtaacgg cttgcagcgg cttgagtagg ctgtcgcgtt
     1201 cggcttgtaa aatcaacatt ttcgctcctt tgaatcggtt tgtatagtgg attaaattta
     1261 aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacgaaaccg attcacttgg
     1321 tacttcagca ccttagagaa tcgttctctg tgagctaagg cgaggcaacg ctgtactggt
     1381 ttaaatttaa tccgctatat ctttaccctt cggacggcat gggcaatatc atgtcgtctg
     1441 aaaacgtttt ccatcagttt tgaatcagaa tcagcagttt ctcgtaatcc tgcgccaact
     1501 cggggtcttc ctcgcgcagt ttcgccaccg ccctgatgcc gtgcatgacg gtcgtatggt
     1561 cgcgtccgcc gaacgaatcg ccgatagacg gcaggctcaa agtggtcagt tctttggtca
     1621 ggctcatggc aacctgacgc ggacgggcaa tgttgcgcgt gcgttttttg ccgagtacgt
     1681 cgctgatttt gatgcggtaa tatttcgcca ccgcatcaat gatgaggtcg gcggtaatga
     1741 ctttgtgctt ctcggcgata atgtcctgca acgccgtacg cgccaaatcc atatcgatga
     1801 cggggcggtt cataaagcgg ctgctcgcgc tgacgcggtt gaatgcgcct tcaagctcgc
     1861 gcacattgga acggatcagg ttggcaataa acaatgccgc ctcgtcttcg atgctgatac
     1921 ccgccgcctc tgcctttttc tgcaaaatgg cgatgcgcat ttccaattcg ggcggctcga
     1981 gttccaaagt caaaccccaa gagaaacgcg atttgagccg gtcgtccatg ccttcgattt
     2041 tggcgggtaa aacatcgcaa gtgaggatga gctgtttttt ctcgttgtgg aaatggttgt
     2101 acagatagaa aaactcttcc atcgtacggt ctttgccttt gatgaactgg atatcgtcga
     2161 taatcagcag gtcgtattgc ttgtattgct gcttgaacac gtcgtaggta ttgttgcgaa
     2221 ccgccttcat aaagctgcgg atgtagtcgt ccgaatgcat atagcgcact ttcgcatcgg
     2281 gacggttttt caaaagctcg ttgccgaccg cctgcacaag gtgggttttg cccaaacccg
     2341 tgctgccata gaggaagaac gggttataac tctgtcccgg actttccgct atcgcctgcg
     2401 cggcggcagc tgcaaggcgg ttgcccttac cttcgaccaa cgtatcaaac gtgtaatccg
     2461 gagacaggtt ggtttgttcg taacgcgcct cttccgcatc acgctgcgca tccgtccgcg
     2521 ctttcgcaag cacctccaat tccggacggg aagcagaccc ggcagcctga cgcggctcgt
     2581 tcggcaggtt tttcatacgt tccgccaaaa tatccgccgc cgttttcgac gcagcgggtt
     2641 tgacaggctc ttcagacggc agctcgtcca acagaacctc ctgcacaggc atctcctccg
     2701 accccgcgtg caaggacggc tcggcaggtt cgacagcacc ttcaaccgcc gccatctcat
     2761 aacgcacgcc ttctcccggt ttgaatacga aggcggaacg gccggcagcc aactcttccc
     2821 tcaccgcttc tatttttccg gcaaactggc tcttgagcat attgcaggca aactggttct
     2881 tgccgtacac cacccatacg ccaccctcct caccgacggt aaggggcgca atccattgcg
     2941 caaactgccc gtgaggcaac atatcgtgaa gacggcggag gcacagcggc caaaactctg
     3001 ctaatgtcat ggataggctc gaatcggtaa aaatgaaatc gaaaacaaag aaaatataat
     3061 attttcaaaa agaaaacaaa tctgttgaac acacatcggt tcaaaatgca gcgaaccgat
     3121 tataccgact cgccgatatt ttatccacaa cccgtgcaaa aatttatcca cagaaaggcg
     3181 gcagaaatcc gcaggcaatc gggcaatcct cctgcaaagt ttctatattg attgacaaaa
     3241 gcggcaaatt ggagtgtaat tcacggttta attatctacc cattctattt ttaggaaaca
     3301 tcatgaaacg cacttatcaa ccttccgtta ccaaacgcaa acgcacccac ggcttcctgg
     3361 tgcgctccaa aacacgcggc ggccgcgcag tactggcagc acgccgtgcc aaaggccgca
     3421 aacgcctggc agtgtaattt tggactaccg cttcggaaag cagtaccgct tgttaaaaac
     3481 ggatgatttt tcatccgttt ttgcattcag aaaccgccgc agccgcgacc tgctgcaagt
     3541 ttcgcgctca aacggcaacg ggctgggcca tccccgcatc ggtctggtga tcggcaaaaa
     3601 aaccgccaaa cgcgccaacg aacgaaatta tatgaagcgc gtcatccgcg actggttcag
     3661 attgaacaaa aaccggctgc cgccgcagga tttcgtcgtg cgcgtccacc gtaaattcga
     3721 caggcctacc gcaaaacagg caagggcgga actggcacaa ctcatgttcg gcaaccccgc
     3781 aaccggatgc aggaaacagg catgatcaga acggtactct gcaggcaagg ttcagacggc
     3841 aacgggtttc ccatacaagg aacatcccga tgaacttcct attgtccaaa ctcctgctgg
     3901 gactgatacg gttctaccaa tattgcatca gcccgctgat tccgccgcgc tgccgttata
     3961 cgccgacctg ttcgcaatac gcggtcgaag cggtcaaaaa atacggcgca ttcaaaggcg
     4021 gtcggctcgc catcaagcgc attgcacgct gccacccttt cggcggacac ggacacgacc
     4081 ccgttccctg acccgacgca atattcaaat tgcacgcttt ccttttattt cccatcggtt
     4141 tctatataat gccgtctgaa acttcgggct ggcggcacga ccgtcgggta tgaagcccac
     4201 ccttattccc cgtctatcgg aacacgcaac ctgcggcatt tccgaccatt caggaaactc
     4261 ttatggattt taaaagactc acggcgtttt tcgccatcgc actggtgatt atgatcggat
     4321 gggaaaagat gttccccact ccgaagcccg tccccgcgcc ccaacaggca gcacaacaac
     4381 aggccgtaac cgcttccgcc gaagccgcgc tcgcgcccgc aacgccgatt accgtaacga
     4441 ccgacacggt tcaagccgtc attgatgaaa aaagcggcga cctgcgccgg ctgaccctgc
     4501 tcaaatacaa agcaaccggc gacgaaaata aaccgttcat cctgtttggc gacggcaaag
     4561 aatacaccta cgtcgcccaa tccgaacttt tggacgcgca gggcaacaac attctaaaag
     4621 gcatcggctt tagcgcaccg aaaaaacagt acagcttgga aggcgacaaa gttgaagtcc
     4681 gcctgagcgc gcctgaaaca cgcggtctga aaatcgacaa agtttatact ttcaccaaag
     4741 gcagctatct ggtcaacgtc cgcttcgaca tcgccaacgg cagcggtcaa accgccaacc
     4801 tgagcgcgga ctaccgcatc gtccgcgacc acagcgaacc cgagggtcaa ggttacttta
     4861 cccactctta cgtcggccct gttgtttata cccctgaagg caacttccaa aaagtcagct
     4921 tctccgactt ggacgacgat gccaaatccg gcaaatccga ggccgaatac atccgcaaaa
     4981 ccccgaccgg ctggctcggc atgattgaac accacttcat gtccacctgg atcctccaac
     5041 ccaaaggcgg acaaagcgtt tgcgccgctg gcgaatgcag catcgacatc aaacgccgca
     5101 acgacaagct gtacagcgca agcgtcagcg ttccccttgc cgccatcccg gccggcgcga
     5161 aagccgaaac cgccgtcaac ctgtatgccg gcccgcaaac cacttccgtc atcgcaaaca
     5221 tcgccgacaa cctgcaactg gccaaagact acggcaaagt acactggttc gcctcccccc
     5281 tcttttggct tttgaaccaa ctgcacaaca tcatcggaaa ctggggctgg gcaatcgtcg
     5341 ttttgaccat catcgtcaaa gccgtcctct acccgctgac caacgcgtcc taccgctcga
     5401 tggcaaaaat gcgcgccgcc gcacccaaac tgcaagccat caaagagaaa tacggcgacg
     5461 accgtatggc gcaacaacaa gcgatgatgc agctttacac agacgagaaa atcaacccgc
     5521 tgggcggctg cctgcctatg ctgttgcaaa tccccgtctt catcggattg tattgggcat
     5581 tgttcgcctc cgtagaattg cgccaggcac cttggctggg ttggattacc gacctcagcc
     5641 gcgccgaccc ctactacatc ctgcccatca ttatggcggc aacgatgttc gcccaaacct
     5701 atctgaaccc gccgccgacc gacccgatgc aggcgaaaat gatgaaaatc atgccgttgg
     5761 ttttctccgt catgttcttc ttcttccctg ccggtctggt attgtactgg gtagtcaaca
     5821 acctcctgac catcgcccag caatggcaca tcaaccgcag catcgaaaaa caacgcaccc
     5881 aaggcgaagt cgtttcctaa atgccgcagc atgaaaaatg ccgtctgaaa cctgttcaga
     5941 cggcattttt attgttcacc ccctatcggg gcggaaatct tcaacctgca tacatcacaa
     6001 aaatcgtcgg gcgttttttc agattgggca tttctttcct tttccgccac tgcgcgattg
     6061 tccggctgat gatttcctgt gtcggcaagg tcaaatccgc agctacacac aagcgcgttt
     6121 cagaatgcag gttttccacc gcatccgcca acaacacatc gttgcgatac ggcgtttcga
     6181 taaacagctg cgtttcattc tgctggcgcg aacgttgctc caaagccttc aaactctgaa
     6241 tccgctcgtt tttttcagac ggcagataac ccttaaacgc aaaactctgc ccgttcgcac
     6301 ccgaagccat caaagccagc agcaggctgg aaggcccgac cagcggacgc acttcaaaac
     6361 cgtgtttatg cgccaaagcc accagattcg cacccggatc ggcaacagcc gggcaacccg
     6421 cctcgctgac aatgcccata ctgcgccctt cttgcaaagg tttcagcaat tccagcaaag
     6481 tcttcaaatc cgtgtgttcg ttcagcgttt gcaaattcag ttcgcggata ggcattgtca
     6541 cgcccaaatg tttcaaatgc gcgcgcgccg ttttttccgc ttccacgaca aaatctgtca
     6601 gcccgacaat cgcctgttgt tcatgcggca acaggcacgg cgtgtcaggc gtacccaaag
     6661 gcgtaggaat caaatacaaa acaggagaca ccattccctc actcatcagt taaaaatgcc
     6721 gtctgagcct ttcagacggc ataaacgggc agttacagaa cctccacgcc ctcatttttc
     6781 aagaaatcga ccagacggaa aaccggcaaa ccgattaaag cattcggatc gctgctttca
     6841 atccgttcaa tcagcagcgc gcccaagccc tcgctcttca acgcacacga gcaataaacc
     6901 gcatcaggct cgcgctccaa atagcggagg atgtgcagct cgtccaactt cctcatcacg
     6961 accaccgtct tatcgatatg ccgttgcatt ctgcccgtaa ccgtatttag cagaacgacc
     7021 gcgctgtaaa actcaatctc cctgccgctc aaatgcatca acatcttttg cgcattagca
     7081 aggttcatcg gcttgcccca ctgcctgccg tcgcaccacg ccacctggtc cgcaccgaca
     7141 atcaacgcct ctgggaaacg cccggtcaac gaccgcgcct taccctcggc aaggcgcaat
     7201 gccgtctgag gggcggattc ccccaacatc ggcgtttcgt caaaatcggg ggacgccgcc
     7261 tgaaaggcaa tgccgagcct ttccatctgt tcgcggcgga aaaccgaact cgtacccaaa
     7321 atcaaaggca gttccaaacc catcccatcc tccttaccgt tgaaaacacg cccgaagggg
     7381 cagtaaaatc cagccatgcg ccgaaacacg gatacccgcc ttcggcgtac cgcaacattt
     7441 ttcttaaaaa tattgacgtt agaacatcta aattatatca tatcccgttt atgtcagacc
     7501 ctaatttgat tgacccggaa atttttgccg ccgaagggca gaacctgcaa ggcagttttc
     7561 tgctggaaga attggatgaa cgcgtcagtt cgcacgatta tcccgccgac aggcagacca
     7621 aaatatcgtt tacactgacc ggcggtcgcg accggctgca acgcctgttc ctcgacctga
     7681 acgtcaaagc cgatatgccc ctgatttgcc agagatgtat caaacccatg ccgttcatgc
     7741 ttgatgaaag cagccgtatc gtcctgtttt ccaacgaaga gtccttggac gaatccatgc
     7801 ttgccgacga agaactcgaa ggcatactga ttgaaaaaga actcgacgtg cgcacattgg
     7861 tagaagacca aatcctgatg tccctgccct tttcgccgcg acacgaagac tgcggcgaca
     7921 atgggacact ggaagaagtc aatcgggaca aacccaaccc ctttgctgtt ttggcaggtt
     7981 tgaaaagcaa ttgattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac
     8041 aaaacaaaaa atccccttcc aaacgcggta tgcaccgttc gcacgacgcg ctgaccgcgc
     8101 ctgcactgtc tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca
     8161 acggtatgta ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact
     8221 gactgaaaaa gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca
     8281 tccgtccaaa ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataataaccc
     8341 gtgaa
//
LOCUS       DAWFIT010000086         8283 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFIT010000086 DAWFIT010000000
VERSION     DAWFIT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.086"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGQ85_001776"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGQ85_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0751147.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WGQ85_001777"
     CDS             complement(1659..2042)
                     /locus_tag="WGQ85_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0751148.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WGQ85_001778"
     CDS             complement(2046..3587)
                     /locus_tag="WGQ85_001778"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0751149.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WGQ85_001779"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WGQ85_001779"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0751150.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4774..5997
                     /locus_tag="WGQ85_001780"
     CDS             4774..5997
                     /locus_tag="WGQ85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0751151.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     STSLLWFSAVIGPILGVTAVFFQRTAQKFPFIKRDNIKIILLAVCMFALVGAVAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6158..7738
                     /gene="purH"
                     /locus_tag="WGQ85_001781"
     CDS             6158..7738
                     /gene="purH"
                     /locus_tag="WGQ85_001781"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0751152.1"
                     /translation="MSVIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIATAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1837 a         2255 c         2356 g         1835 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacatcac gctgttttcg ttgaagaaca gtgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 cacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcggggtt
      421 gacggtgtcg ttcgcaccga tgaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaaaacga tgtcgtaggg gactttggct tcggcgagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcgg tgccgttttt
      601 acgtaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacat tgtttgcgcc tttcagcatt tcggcaatat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat aatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 catgaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctacc gttaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gtttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc tcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 tttttaatgc cgtctgaact gttcagacgg catctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcccgcccac atcagcgagc gcaatgctgc tttgtctgcg
     1741 atgtagcacc agataaaggc gaggcggaac aggatgaaca ggccggcaag cgtgttgacg
     1801 gttgcttgtc cggcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgcgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataagcag gcaatcagga tgcaccaata ggcaaaagtc
     2041 atgctttatc ctttcctaaa catattcaac atgcggcgcg ttacgaagaa gccgccgaag
     2101 atattgatgc tggcaatcag gatggcgatg aaggcaagca gggtaacgaa accgttgcct
     2161 tggctgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggttaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggctgcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg cgccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtgatt
     3121 tggccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccatg
     3241 tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ctcgccttcg
     3361 gacggcgcat tgaccttata aatcaaaggg caggcccaaa ccgccgcttt gtcggcaacg
     3421 gtcgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg gggcagggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tttccgattg cagtcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagtt accgccatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga acctgattac
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cgaggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcgac
     4081 gaactcggtt taattgtcag cgatatggat tcgacgctga ttaccattga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaagtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 ttcttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcgggttc
     4381 acatttttta ccgaaaggct gcaacaacga ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggt cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc gcatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agcgcgggcc gccgccgatg cctgtatcaa cttcggcggt
     4681 ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgcc gtctgaaagg
     4741 ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacaac atacggcata cggttatggc acggacggcg tgtccatctc gttccgcgaa
     4921 ggtgtggcac aggcttccgg tatgcggcgc gttgccgtgc tgacgctgtg cggcgcggtc
     4981 gcaggcagcg gctggtggtt gctgaaacgt ttcggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaaac agccgttgca ggggctgccg tttctgacga cggttttcca tgttctgctg
     5101 caaatcataa cggtcggact cggttcaccg ctcggacggg aagtcgcccc gcgcgaaatg
     5161 accgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggacgaagg cgaaatgcgg
     5221 ctgctgattg cttgcgcttc gggtgcgggt ttggcggccg tgtataacgt gccgctcgcc
     5281 tccacgctgt tcatcctcga agccatgctg ggcgtgtgga cgcagcaagc cgtcgccgcc
     5341 gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca tcggcttagg cgacgtgcag
     5401 caatatcatc ccgccaacct tgccgtcagc acttcattac tttggttttc cgccgtcatc
     5461 ggtccgatac tgggcgtaac cgccgtcttt ttccaacgta ccgcccaaaa attccccttt
     5521 atcaagcgcg acaatatcaa aatcattctc ttggccgtct gtatgtttgc actcgtcggc
     5581 gcggttgccg tttggtttcc cgaagttttg ggcaacggca aagcaggcaa ccaactgacc
     5641 tttggcggct tgaccgactg gcaacacagc cttgagctga ccgccgtcaa atggctggtc
     5701 gtattgatgg cacttgccgc cggtgcatac ggcggcctga ttacgccgtc gatgatgctc
     5761 ggcagcacca tttcttttgc tgccgccgcc gcgtggaaca gcgtcttccc cgaaatgccg
     5821 tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtatcgct caaaatgccg
     5881 ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct gctgctgcct
     5941 ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     6001 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg tcaacaatat
     6061 ctgcaaaaac ttactgccaa aaaactgaaa atagcggata ataccgcccc gaaaaagttc
     6121 ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     6181 gatcagcctg tccgacaaga cgggcgcagt cgaatttgcc caaaccctgc acaagctcgg
     6241 tgtcgaaatc ctctctaccg gcggcacggc gaagctgctt gccgatgcgg gcgttcccgt
     6301 gattgaagtt gccgactaca ccggtttccc cgaaatgctc gacggccgcg tgaaaacgct
     6361 gcatccgaaa atccacggcg gtattttggg ccggcgcgat ttgcctgaac acgtcgccaa
     6421 gatggaagaa cacggcatcg gcaacatcga cctcgtgtgc gtcaacctct accccttcgc
     6481 tgccaccatc gccaaaccaa actgcacgct ggaagacgcg attgaaaaca tcgacatcgg
     6541 cggcccgacc atggtgcgct ctgccgcgaa aaactggaaa cacgtcgcca tcgttactga
     6601 cacagccgat ttcccggcca tagctgccga actcgaagcc aacaacggcg cattgagcga
     6661 caaaacccgt ttcaacctgt cgcgcaaagc gttcagccac accgcccaat acgacggcat
     6721 gatttccaac tacctgacct cgctttcaga cgacgttttg agcggagagc ctgaaatcgg
     6781 cgaatttcca agtcggttca accaaagctg gattaaagtg caagacatgc gctacggcga
     6841 aaacccgcat cagcgcgccg cgttctaccg cgatattgac cccgccgcag gcagcctttc
     6901 cgcctacaaa caactgcaag gcaaagaatt gtcgtacaac aacatcgccg atgccgatgc
     6961 cgcatgggaa gccgtcaaat cctttgacgc gccagcctgt gtgattgtga aacacgccaa
     7021 tccgtgcggc gtagctgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     7081 cgacaccacc agcgcgttcg gcggcatcat cgctttcaac cgcgaagtgg acggcgaaac
     7141 cgtcaaacaa attaccgaca accagtttat ggaagtcctg atggcgccga agtttaccgc
     7201 cgaagccctc gaaattgcca ccgccaagaa aaacgtgcgc gtgttggaag tgcctttgga
     7261 agcaggcgcg aaccgcttcg aactcaaacg cgtcggcggc ggactgttgg tacaaacccc
     7321 cgacattcac cgcctcaacc gcgccgattt gaaagtcgtt tccaaacgcc aaccgaccga
     7381 gcaagaatgg aacgacctga tgtttgtttg gaacgtcgca aaatacgtca aatccaacgc
     7441 catcgtcttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     7501 ggacagtacc cgcatcgccg cccgcaaagc gcaggacgca ggcttggact taaacggcgc
     7561 atgtgccgcc tctgatgctt tcttcccctt ccgcgacggc gtggacgtga ttgccgagca
     7621 gggcatcaaa gccatcatcc atccggcagg ttcgatgcgc gatcaggaag tcttcgacgc
     7681 agcggacgaa cacggcatcg ccatggtcgt aaccgacgtg cgccacttcc gccattaacg
     7741 cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg ctattttgtg
     7801 aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt gttgtatagg
     7861 gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct gcatcaaaaa
     7921 tttttggaga atcggcggga gtcggcggtt ttggtttcgg cggggacggt ggaaatgggt
     7981 aacattgacg gaatcgacgg aagcggtggg cttcagccca ccgcttgtat attggaatca
     8041 ccgtatcata gcgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag
     8101 cggcagggca acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg
     8161 caggtttatg atgagtaccc aataccatta cgcaggtata gtgaattaaa tctaaaccag
     8221 tacagcgttg gttcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
     8281 tga
//
LOCUS       DAWFIT010000087         8274 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFIT010000087 DAWFIT010000000
VERSION     DAWFIT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.087"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            78..2168
                     /locus_tag="WGQ85_001782"
                     /pseudo
     CDS             78..2168
                     /locus_tag="WGQ85_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2410..3876
                     /locus_tag="WGQ85_001783"
     CDS             2410..3876
                     /locus_tag="WGQ85_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0751153.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNSGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4077..5045)
                     /locus_tag="WGQ85_001784"
     CDS             complement(4077..5045)
                     /locus_tag="WGQ85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751154.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5123..6415
                     /locus_tag="WGQ85_001785"
     CDS             5123..6415
                     /locus_tag="WGQ85_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0751155.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(6918..7604)
                     /locus_tag="WGQ85_001786"
     CDS             complement(6918..7604)
                     /locus_tag="WGQ85_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0751156.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7679..8152)
                     /gene="queF"
                     /locus_tag="WGQ85_001787"
     CDS             complement(7679..8152)
                     /gene="queF"
                     /locus_tag="WGQ85_001787"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0751157.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(8156..8200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         1979 a         2038 c         2467 g         1790 t
ORIGIN      
        1 gcttttcctg atttttcatt gcgatgtagt ataatgtagt atataatcat tataatttta
       61 acacttgaca aaggaaaatt tctcatgaca ctgaaagcaa gcaagcaagc aagcaagcaa
      121 gcggtcgggt taatctatta acattatctg ttttatcgct gttttgcacg ccatatgttt
      181 gaggttcgga tgcgtacgat cccgtcaaag aagccgagat taaaaacaaa tttattttag
      241 aagcggcgga agacagaaat tcccacgttt ggcgcggccc gtgcagcata tcttttgatt
      301 gcttcggtat gttcagagct cagcttggtt caaatactcg ttctaccaaa atcggcgacg
      361 atgccgattt ttcattttca gacaagccga aacccggcac ttcccattat ttttccagcg
      421 gtaaaaccga tcaaaattca tccgaatatg ggtatgacga aatcaatatc caaggtaaaa
      481 actacaatag cggcatactc gccgtcgata atatgcccgt tgttaagaaa tatattacag
      541 atacttacgg ggataattta aaggatgcgg ttaagaagca attacaggat ttatacaaaa
      601 caagacccga agcttgggaa gaaaataaaa aacggactga ggaggcgtat atagaacagc
      661 ttggaccaaa atttagtata ctcaaacaga aaaaccccga tttaattaat aaattggtag
      721 aagattccgt actcactcct catagtaata catcacagac tagtctcaac aacatcttca
      781 ataaaaaatt acacgtcaaa atcgaaaaca aatcccacgt cgccggacag gtgttggaac
      841 tgaccaagat gacgctgaaa gattcccttt gggaaccgcg ccgccattcc gacatccata
      901 tgctggaaac ttccgataat gcccggatcc gcctgaacac gaaagatgaa aaactgaccg
      961 tccataaagc gtatcagggc ggcgcggatt tcctgttcgg ctacgacgtg cgggagtcgg
     1021 acgaacccgc cctgaccttt gaagacaaag tcagcggaca atccggcgtg gttttggaac
     1081 gccggtcgga aaatctgaaa acgctcgacg ggcgcaaact gattgcggcg gaaaaggcag
     1141 accctaattc gtttgcgttt aaacaaaatt accggcaggg actgtacgaa ttattgctca
     1201 agcaatgcga aggcggattt tgcttgggcg tgcagcgttt ggctatcccc gaggcggaag
     1261 cggttttata tgcccaacag gcttatgcgg caaatacttt gtttgggctg cgtgccgccg
     1321 acaggggaga cgacgtgtat gccgccgatc cgtcccgtca aaaattgtgg ctgcgcttca
     1381 tcggcggccg gtcgcatcaa aatatacggg gcggcgcggc tgcggacggg cggcgcaaag
     1441 gcgtgcaaat cggcggcgag gtgtttgtac ggcaaaatga aggcagccgg ctggcaatcg
     1501 gcgtgatggg cggcagggcc ggccagcacg catcagtcaa cggcaaaggc ggtgcggcag
     1561 gcagttattt gcatggttat ggcgggggtg tttatgctgc gtggcatcag ttgcgcgata
     1621 aacaaacggg tgcgtatttg gacggctggt tgcaatacca acgtttcaaa caccgcatca
     1681 atgatgaaaa ccgtgcggaa cgctacaaaa ccaaaggttg gacggcttct gtcgaaggcg
     1741 gctacaacac gcttgtggcg gaaggcgttg tcggaaaagg caataatgtg cggttttacc
     1801 tgcaaccgca ggcgcagttt acctacttgg gcgtaaacgg cggctttacc gacagcgagg
     1861 ggacggcggt cggactgctc ggcagcggtc agtggcaaag ccgcgccggc attcgggcaa
     1921 aaacccgttt tgctttgcgt aacggtgtca atcttcagcc ttttgccgct tttaatgttt
     1981 tgcacaggtc aaaatctttc ggcgtggaaa tggacggcga aaaacagacg ctggcaggca
     2041 ggacggcact cgaagggcgg ttcggcattg aagccggttg gaaggattat ttgtctgcgc
     2101 gtctcggata cggcaaaaga tcggacggcg ataaagaagc cgcattgtcg gtcaaatggc
     2161 tgttttgatg cgtcgggaaa tgttttgacg cacaggcggc acaccggcac ggccccgcgc
     2221 gccgccccgc aaaccaatcc gaaccctgcc gccccgaagg gcggggcatc atgatgaaac
     2281 cggcggaaaa ccgccggttt tttgccgccg tttgaaaccc gattctggct tcagacggca
     2341 ttgtcgcggc atcgggcggc agggtttgga acagcggcat aaaaaactga tacaatccgc
     2401 cgattgataa tggttatttt ttatttttgt gggaagacat ttatgcctgc acgaaacaga
     2461 tggatgctgc tgctgccttt attggcaagc gcggcatatg ccgaagaaac accgcgcgaa
     2521 ccggatttga gaagccgtcc cgagttcagg cttcatgaag cggaggtcaa accaatcgac
     2581 agggagaagg taccggggca ggtgcgggaa aaaggaaaag ttttgcagat tgacggagaa
     2641 accctgctga aaaatcccga attgttgtcc cgcgcgatgt attccgcagt ggtctcaaac
     2701 aatattgccg gtatccgcgt tattttgccg atttacctac aacaggcgca gcaggataag
     2761 atgttggcac tttatgcaca agggattttg gcgcaggcag acggtagggt gaaggaggcg
     2821 atttcccatt accgggaatt gattgccgcc caacccgacg cgcccgccgt ccgtatgcgt
     2881 ttggcggcag cattgtttga agacaggcag aacgaggcgg cggcagacca gttcgaccgc
     2941 ctgaaggcgg aaaacctgcc gccgcagctg atggagcagg tcgagctgta ccgcaaggca
     3001 ttgcgcgaac gcgatgcgtg gaaggtaaac agcggcttca gcgttacccg cgaacacaat
     3061 atcaaccaag ccccgaaacg gcagcagtac ggcaaatgga ctttcccgaa acaggtggac
     3121 ggcacggcgg tcaattaccg gctcggcgcg gagaaaaaat ggtcgctgaa aaacggctgg
     3181 tacacgacgg cgggcggcga cgtgtccggc agggtttatc cggggaataa gaaattcaac
     3241 gatatgacgg caggcgtttc cggcggcatc ggttttgccg accggcgcaa agatgccggg
     3301 ctggcagtgt tccacgaacg ccgcacctac ggcaacgacg cttattctta caccaacggc
     3361 gcacgccttt atttcaaccg ttggcaaacc ccgaaatggc aaacgttgtc ttcggcggag
     3421 tgggggcgtt tgaagaatac gcgccgggcg cgttccgaca atacccattt gcaaatttcc
     3481 aattcgctgg tgttttaccg gaatgcgcgc caatattgga tgggcggttt ggatttttac
     3541 cgcgagcgca accccgccga ccggggcgac aatttcaacc gttacggcct gcgctttgcc
     3601 tgggggcagg aatggggcgg cagcggcctg tcttcgctgt tgcgcctcgg cgcggcgaaa
     3661 cggcattatg aaaaacccgg ctttttcagc ggttttaaag gggaaaggcg cagggataaa
     3721 gaattgaaca catccttgag cctttggcac cgggcattgc atttcaaagg catcacgccg
     3781 cgcctgacgt tgtcgcaccg cgaaacgcgg agtaacgatg tgttcaacga atacgagaaa
     3841 aatcgggcgt ttgtcgagtt taataaaacg ttctgattgc tgttcctttt cggaggaaac
     3901 cctgccggcg gcggtatcac ggcgggcatc ggcggctttc gggcggtgct ttgcgtgccg
     3961 ccgcgtgtgc ggaaacgcat tccggttttt ccggcataac ggcgatgcga ggtaaaatgc
     4021 cgtctgaaac ccgattcggg cttcagacgg cattgtcgcg gttgcggcag gcgggttcac
     4081 cagattccgt caaaggtttt cgcgccgcgc caaaatttcc acctgtcggc gggtttgaag
     4141 gtcagcgtac cgccgtgttg tccgtccgtg gtgatgtcca gccgtttgat tttgccgatg
     4201 cggacggctt cgtaaagcgg tgcgaaccag cgttcttccc actgctgcaa tattgccgca
     4261 taccgctccc tgtcccctgt cagggcggtc aggcgcaaat cgtccataaa caggatatgg
     4321 tgcgtgtcgg gtaggtatgc cgccgtttct tcataggcgc ggaagttgtc gggcaatgcg
     4381 cggcggtcgg ggtggaaacg gctccaaacc gtatcggcga aaagcgtgcc gccttgactg
     4441 cttccgtgca gactgtccca aagccacagc ccgttcaact cgggcagccc gcgtttcttg
     4501 cggttatggt tgacggggtg cgccgccagc cacatttgga tttcggtttg gacgcgcagc
     4561 cattccaacg cgtcttcccc gtccggctga tcgtccgcgc ccaacaatcc gcccaagtcc
     4621 aaaacgggct tcgcgcccca gcggtacgcg caaggaaggg aaaccagcca taattcgggc
     4681 aggacgggaa cgaaacgcca tggaatgtcg ccgtaaaacg ccgacaggtc gcggcagagc
     4741 cgttccgctt catccgtacc gacgttcaga tattccgccg ttagcacatt tgcctgatgc
     4801 atccccatct tttgccagac gggcgtggcg agcgcgacgg cttcagacgg catattcagg
     4861 ctttgtgccg cgcgttccgc cagtctgccg caccacaaat aacgcgcgta aaatgccgaa
     4921 gccgtgcagc tttggcggtg cagcgagccg tattgcagga ttttgttgaa ggcgggcagg
     4981 cagagaggta ttcgggtttc gtcttcatcc aaattgagcg agggaatggc gagggtgagt
     5041 ttcatcgttt gacgtttcag aaatgcaggt caggcgcaac attatagagg attcggcgca
     5101 aacgccgtca aaaaggaaca atatggctgt cttcccactt tcggcaaaac atcggaaata
     5161 cgcgctgcgc gcgcttgccg tttcgattat tttggtgtcg gcggcataca ttgcttcgac
     5221 agagaggacg gagcgcgtca gaccgcagcg cgtggaacaa aaactgccgc cgctgtcttg
     5281 gggcggcagc ggtgttcaga cggcatattg ggtgcaggag gcggtgcagc caggcgactc
     5341 gctggcggac gtgctggcgc gttcgggtat ggcgcgggac gaaattgccc gaataacgga
     5401 aaaatatggc ggcgaagccg atttgcggca tttgcgtgcc gaccagtcgg ttcatgtttt
     5461 ggtcggcggc gacggcggcg cgcgcgaagt gcagtttttt accgacgaag acggcgagcg
     5521 caatctggtc gctttggaaa aaaaaggcgg catatggcgg cggtcggctt ctgaggcgga
     5581 tatgaaggtt ttgccgacgc tgcgttcggt cgtggtcaaa acgtcggcgc gcggttcgct
     5641 ggcgcgggcg gaagtgcccg tcgaaatccg cgaatcctta agcgggattt tcgccggccg
     5701 cttcagcctt gacggtttga aggaaggcga tgccgtgcgc ctgatttacg acagcctgta
     5761 tttccacggg cagcaggtgg cggcgggcga tattttggcg gctgaagtcg ttaagggcgg
     5821 cacaaggcat caggcgttct attaccgttc ggacaaggaa ggcggagggg gcggcaatta
     5881 ttacgatgaa gacggcaggg tgttgcagga aaaaggcggc ttcaacatcg agccgctggt
     5941 ctatacgcgc atttcttcgc cgttcggcta ccgcatgcac cccatcctgc acacttggcg
     6001 gctgcacacg ggcatcgatt atgccgcacc gcagggaacg ccggtcaggg cttccgccga
     6061 cggcgtgatt acctttaaag gccggaaggg cggatacggc aacgcggtga tgatacgcca
     6121 cgccaacggt gtggaaacgc tgtacgcgca cttgagcgcg ttttcgcagg cgcaaggcaa
     6181 tgtgcgcggc ggcgaggtca tcggttttgt cggttcgaca gggcgttcga ccgggccgca
     6241 cctgcattac gaggcacgca tcaacgggca gcccgtcaat cctgtttcag tcgcattgcc
     6301 gacacccgaa ttgacgcagg cggacaaggc ggcgtttgcc gcgcagaaac agaaggcgga
     6361 cgcgctgctt gcgcgcttgc gcggcatacc ggttaccgtg tcgcaatcgg attgaagttt
     6421 gaaccggcga cgaaaacaat gccgtctgaa aatctgcaaa caggttttca gacggcattt
     6481 atagtggatt aacaaaaacc agtacgtcgt tgcctcgcct tagctcaaag agaacgattc
     6541 tctaagatgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
     6601 gttgccttgt cctgattttt gttaatccac catacagtcg attaaaataa aactaagcca
     6661 aggaagcact gccgtcattc ccgtacgggc gggaatccgg acaccacggt acggaaaccc
     6721 atccgccgtc attcccgcga aagcgggaat ctagaaatgc aacgcggcag gagtttatcg
     6781 gaaatgatgg aaacccaacg caccggattc ccactttcgc gggaatgacg aagtaggtag
     6841 gaatccggat ttatccgttc cgacagtgtt tgcaaataaa agaaaaccca accgtcccga
     6901 ttcccggcag ggctgtttta cggattttgc agcgagggcg cggggcggtc ttgcgcctgt
     6961 ttggtttgca gggtcgtcag ttttttcgtc agcagattca gaatcacgcc gtaggcgggc
     7021 aggaaaaaca gaccgcagac ggtgagtttg aacaggtaat cgacaaaagc gatgccctgc
     7081 cagtttgccg ccataaattc atcgctgctt gcatagaagg caacggcgaa aaataccaac
     7141 gtatccaagg cgttgccgat gacggttgat gcggtcggtg caatccacca cgctttcaga
     7201 cgacgtaatt tgttgaacac aaaaatatca aggatttgtc cgagcgcgta ggcggcaaag
     7261 ctggccaagg cgatgcgtcc gacaaaggtg ttgaattcgg atagtgcgcc caagcctgtc
     7321 caactgccgt tgtggaacaa aacggaaaag acgtaggaaa gcaaaagggc ggggaacatc
     7381 acccaaaaga taatccgccg tgccaagtgc gaaccgaaaa tgcggacggt caggtcggtg
     7441 gcgaggaaga tgaaggggaa ggaaaacgca ccccaagtgg tgtggatgcc gaaaatttgg
     7501 aaggggaact gcaccagata gttgctggcg gcgatgatga ggatatggaa aagcaccagc
     7561 cagaagagtg ccttctgttg ctgtgcggca gtaaatgcgt acataaaaat ctttcggaaa
     7621 ggcgttcaga cggcataccg tatcgaagga atgccgtctg aaatatggga aggatggttt
     7681 attgtgcgtc gtgctcaaac aggcgtttac gcgccaatgc ttcaaactcg gtgcctgctt
     7741 tgccgtaatt ggcgaaagga tgaatggcga tgccgccgcg cggtgtgaac tcgccgaata
     7801 cttcgatgta tttcggatcc atcagggcaa tgaggtcttt catgatgatg ttgacgcagt
     7861 cttcatgaaa atcgccgtgg ttgcggaagc tgaagaggta gagtttcagg gatttgcttt
     7921 ccaccatttt gatgtgcgga atgtagcgga tgtagatggt ggcgaagtcg ggctgcccgg
     7981 tcatggggca gaggctggtg aactctgggc agacgaattt gacgaaatag tcgttgtcgg
     8041 gatgtttgtt gtcgaatgct tcgagaattt caggcgcgta gccggtcgga tatcgggttt
     8101 tctgattacc caaaagcgag atgccttgca gctcttcggt attgcgggac atagggtttc
     8161 cttagttttt tagtgtggga ggttttcgaa ccacggggcg gcgattgtaa tataagcggc
     8221 ggtatctgtg tagttttctt cagacggcat ggtttggacg gcggcgtttt ccgt
//
LOCUS       DAWFIT010000088         8155 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFIT010000088 DAWFIT010000000
VERSION     DAWFIT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.088"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(214..924)
                     /gene="rmpM"
                     /locus_tag="WGQ85_001788"
     CDS             complement(214..924)
                     /gene="rmpM"
                     /locus_tag="WGQ85_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0751158.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPAPAVVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVANNLV
                     SNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPAHNHHQH"
     gene            1288..2238
                     /locus_tag="WGQ85_001789"
     CDS             1288..2238
                     /locus_tag="WGQ85_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0751159.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2351..3085)
                     /gene="fnr"
                     /locus_tag="WGQ85_001790"
     CDS             complement(2351..3085)
                     /gene="fnr"
                     /locus_tag="WGQ85_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0751160.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3290..4711
                     /gene="hemN"
                     /locus_tag="WGQ85_001791"
     CDS             3290..4711
                     /gene="hemN"
                     /locus_tag="WGQ85_001791"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0751161.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5064..6638)
                     /locus_tag="WGQ85_001792"
     CDS             complement(5064..6638)
                     /locus_tag="WGQ85_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0751162.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6963..8063
                     /locus_tag="WGQ85_001793"
     CDS             6963..8063
                     /locus_tag="WGQ85_001793"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0751163.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
BASE COUNT         2059 a         2280 c         1999 g         1817 t
ORIGIN      
        1 ggcaatggga cacaaacctg aatctgaatc tgaatctgaa cctgcacccc gattctgacg
       61 atagccgttt cagaaaacca cccaaaaaaa caaaccccgc aaaagcgggg gtttgtttca
      121 catcatcgat gccgaccgac gtttcacgaa tattgcaaca gtacctggta caaaatccac
      181 taacctcatg catcggcaag atattgccta gccttagtgt tggtgatgat tgtgtgccgg
      241 cacaacctga cgggttacga tgctgcggat tttcacatcc acacggcggt caggttcgat
      301 acatgcaatc agagcctcac gttttttggc tttagagact ttcgcaccca gtttggcaac
      361 ttcggcttca caaacttgag tcatttgcgc ttgagattcg cccaagccga cagcagaaat
      421 tctagaaaca ggtacgccgt tgctgaccag gttgtttgcc actacgtatg cgcggcgttc
      481 ggacagggcc tgattgtatt tgtcagaacc cataaagtcg gtatggcctt cgacgcggac
      541 agattggata ttggtttgac ccaggcgttg cgccaatact ttcaggttgt cttgagcttc
      601 ggcgcgcaat gaatccttat cgaaaccgaa cagggttttg gcagacaggg aaatggtttc
      661 atcaacatat tgcggagcct gctccacaac gacggcaggc gcgggctcgg gttcgggggc
      721 agcaaccgca tcgccgcatt ctacgcgacc ttggcttgct ttatcaaagt aggcgttttt
      781 ccagcattcg ccatagttgt tgcgtacgat ttcgttcgac tggccgctta cggtgtaacc
      841 ctgaacggac gcctcgcccg caacagcagt gccggaagcg agcaatgcaa cgaataatgc
      901 gcttaatttc agctgtttgg tcattttatt ccctcattaa atttgtacag cagatacaga
      961 agcaactgct gaattgccaa catacaggcc gctgtagaaa catggcggca aatcggatat
     1021 ttcagtctcc actataaaga agcaggggga ggactgtcaa tactcaacgc ctgaaaacaa
     1081 ccgtaaaaca gcctgtttcc tgatagtcta tcatgccgaa aaatcaattc cgtgtcatta
     1141 ctttaggggg attttttcca tatcgcacga ttgccgttgc acaaaaacaa caaacacaac
     1201 ggcaaagccc cataccgtac cggcaagaaa atatgataaa ttataaacaa tgttcagcca
     1261 cccgacacag acccacaccg acccgccatg aaattacaac aattgaaata cgccttagaa
     1321 gtttaccggc acaacctgaa cgtttccgaa gcggccgaag ccttgtttac ttcgcaaccc
     1381 ggcatctcca aacaaatcaa attgctggaa gaagaaatcg gcattcagat ttttatccga
     1441 agcggcaagc gcgtggtttc ggtctcgcag ccgggcaagg tggttttgga tattgcggaa
     1501 cgtattttac gcgatgtaca gaacattaaa aatatcggca gcgagtttac cggacaggac
     1561 agcggttcgc tgacggttgc cacgacgcat actcaggcac gctatgccct acccttgatt
     1621 gttgccgatt ttgtgaaacg ctatccgaaa gtcaacctga ccatcaaaca ggggagccct
     1681 gccgccatcg cacgtatggt tacctcaggc gaggcggact tggcgattgt tacggaacgg
     1741 atagacgacc atcccgaact gggaaaactt ccctgctatg actggactca tgcggttatc
     1801 gtaccgaacg accacccctt gctcgaatgc agaaaccccc tccgcattga agatttggcg
     1861 aggtttccgc tgattactta tgaatttgca ttcaatgcgg gcagcagcat cgcgcgggca
     1921 ttttccaaag cccgtttgga acagccggat gtcgcattgg cggcggcgga tacggacgta
     1981 ttgaagactt atgtgcgctt gggtttgggc gtgggactga tggcgaaaat ggcgtacaac
     2041 ccggatacgg acggcgattt gcagcttgtg gatgcggcac acctgttcga gccgtcgccg
     2101 acgtggattg ctttgcgcag cgatacttat ttgcgcggat atgcctacga ctttatccaa
     2161 gcgtttgcgc cgcacctgac acgcgagaag gtggatagga ttctgtacac gcccatcagc
     2221 gaggattttt cgatttaggc ggctgccggt tttcagacgg cactttgcgg cagatacaac
     2281 aaacaggaca gatgttttcg tctgccctgt gtttattgag aatgccgtct gaaatgttcg
     2341 tacgggttaa tcaaatggcg tgcgaacaac cggacaccat cttcttcaac acctgcagat
     2401 tgaggatctt gatgtgtttg tgttcgacgg aaatcaatcc ttcctgatga aatttagata
     2461 atgtgcggct gacggtttca agtttcagcc cgagataact gccgatttct tcgcgggaca
     2521 ttcttaagat gaagtcgttg gcagcaaaac ctcgggaata aaggcgttgg gaaaggttca
     2581 gcaggaaggc ggcaatccgc tcttcggcgc gcatattgcc caacagcagc ataacacctt
     2641 ggtcgcgcac gatttcacgg ctcatcatgc ggaagaagtg cgtacgcagg ctggggatgt
     2701 tttgccccag ttcttcgatg tgggtaaacg gcagttcgca cacttcgctg tcttccaagg
     2761 cgaccgcgtc gcaactgtgc acatgggaac agatgccgtc catgccgatg agttcgcccg
     2821 acataaagaa acccgttacc tgatcgcggc cgtcctgact ggcgacggtt gttttgaaga
     2881 agcccgaacg gatggcaaag agcgaggtaa aggcttcgcc gacacagaac aggtattcgc
     2941 cctttttcag gcggcggctt tgacggatga cggcatcgag ttggctgagc tcgttgggca
     3001 gcagcccgac aggcaggcag agttcccgca aagaacagga agaacacagc gttttcatct
     3061 gatgtgtagt attatgcgaa gccataccgt acctttttgt gcgctttgcc ccatcatgat
     3121 tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgtcctta tttaaattta
     3181 atccactata tgtgcttatt gacacatatc aagacaggtt tatcatactg tggcattcta
     3241 ccaaactatc caaaatttga ctaacatcat aaaaccgcac acacacacca tgaaaatcat
     3301 tcagatacag aacaatcaca atgtaaacga tgaccgcccc gagtttgacc gcgcgctgat
     3361 tgccagcctg cccgccagcg gcccgcgcta cacttcctac cctaccgccg accgtttcca
     3421 tgacggtttc cgcgaaggcg aatatatcaa agctttacat ttgcgcggta tgggagcgtt
     3481 aaacaaaccg ctttcccttt acattcacat tccgttctgc aacaccatct gctactactg
     3541 cggctgcaac aaaatcatca ccaaagacaa aagccgcgcc gatgcctaca tcgaatatct
     3601 tgaaaaagaa atggaactgc tcgctccaca tctgaacgga cggcaccagc ttgcccaact
     3661 gcacttcggc ggcggcacgc cgaccttttt gagcgacgaa cagatcgaac gtgtcttccg
     3721 catgatacgc aaacatttcg agttaatccc caccggcgaa tactccatcg aaatcgaccc
     3781 gcgcaaagtc agccgcgaca ccgtcctcat gctcggcaga ctcggcttca accgcatgag
     3841 catcggcatt caggatttcg accccaaagt gcaggcggcg gtcaaccgca tccaaagtta
     3901 cgaagaaacc aaagaagtca tcgatgcggc gcgcgaagcg gggttcaaat ccgtcagcgt
     3961 cgatttgatt tacggcctgc cgcaccagac ttcggaaagc atcaaaacca ccatcgatac
     4021 cgttttgtcg ctcgatcccg accgcctcgc cctttatcac tacgcccacc tgccgcacgt
     4081 gttcaaaccg caacgccgca tcgataccgc cgccgttccc gacagcgaag agaagctcga
     4141 tatgctgcaa tactgcgtcc aaaccctaac cgaacgcggc tacgtcttca tcggcatgga
     4201 tcatttcgcc aaacctgacg acgaactctc catcgccctc aaagaaggct tcctccagcg
     4261 caacttccaa ggctattcga cctacgcgga ttgcgatttg gtcgccatcg gcgtgtcgtc
     4321 catcggcaaa atcggcagca cctattccca aaacgaacgc gacatcgatg cctactatgc
     4381 cgccatcgac gaaggcagac tgcccatcat gcgcggctac cagctcaatc aggacgacat
     4441 cctgcgccgc aacatcattc aggatttgat gtgccgcttc gcgctcgact atcggattta
     4501 cgaaagtatg ttcggcatcc cgttcgaccg ctacttcaaa gacgaactgg cggatttgga
     4561 aaaactcgcc ggtttgggat tggtgcgcct gaacagccac gggctgaccg ttaccccgaa
     4621 aggacgcttc ctcatccgca acatcgccat ggtcttcgat taccacctgc gccataaaga
     4681 aaccaaggcg aaatattcgc aaacggtgtg attgcgtcta acgtacaaat gccgtctgaa
     4741 aggctttttc agacggcatt ttgctgccgg caggataagt gttttcaaga acaggcggcg
     4801 gcatatcata acgttccgca cctttgtatc cgaccgttcc gaaaccaaga tatagtggat
     4861 taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact atctgtactg tctgcggctt
     4921 cgtcgccttg tcctgatttt tgttaatcac tataccaaac ggcatatccc gacagaacag
     4981 attgtgcata aggcacaagc ccgcacattc catcaacaaa atgccgtctg aacacgggtt
     5041 cagacggcat cagtatttta caatcagaat actgcctgta aaaccaagta gcagacaacc
     5101 gacaatacgg cggcggcagg cagggtaatg acccacgcca aaccgatggg cttcatcagt
     5161 ttccagttgg cattgcggtt gaccagaccg atgccgagta ccgcgccgac caagatatgc
     5221 gtactggaca cgggcagccc catcagcgac gcgcccatca cgacggatgc ggcggacagt
     5281 tcggcggtaa aacccgaagc aggatgcatt tccgccaaac tcgtaccgac ggttttaatc
     5341 acctctttac cgacaaacca caaaccgaca atcagcgcga taccgaaagt cagcatcgca
     5401 atcgggggga cgacattttg cgcggcaacg ctgttggtac gcaaaacatc cataatcgcg
     5461 gcaaacggac cgatggcgtt ggcgatatcg ttcgcaccgt ggctgaatgc gaagccgcag
     5521 gcggtaaaga cctgcatcca tgaaaacatc tgaaaggtcg atttgcccaa gtctttacgc
     5581 ttgaggcttt tggcaaacac aaacgtcccc atccacaccg ccgcgcctat cataaagatg
     5641 gtcaggaagc tgttgacgtt gctcatcccc aaatgcaggt ttttcaagcc cttgaaaatc
     5701 agcatagcgg aaatcatcat cgcgccgaac gaagcgataa agggaatcca agaatgcagc
     5761 gctttgtagg aatcgacatt gtttttacgg ttgtcgaacg cataaagacc gcggtaatac
     5821 tccgattgca gctcttgcgg atcgaattcg ggttcgtcgt aaatttgcgc gtcgtgcgcc
     5881 attttggtgg cgtactcgac tttttcggct tcggacaaac cctcgaaaaa caggcggtgc
     5941 cgttctttat aggccttttt ttcctgcttg atgcccttga gcgtgccttc cgcccaagcg
     6001 ttgtaatcta agacgttttt cttgacgcgc gaaaacagaa aataggacac cgcgccgccc
     6061 aacacgggcg acaataccca agaaacacca ataccgccca gcttgcccca acgtatcaaa
     6121 tcgcccgatg cggcatcgtt cattaccgcc atacataccg cgctgccgac aatgccgccg
     6181 ataatggaat gggtggtaga taccggaagc ccttttttcg aggcaaacaa cagccacaac
     6241 gccgccgcca aaagcgcgga catcataata aacacaaact gtatgggttc gaaatcaaca
     6301 cccttcaaat cgacgatgcc tttgcgtatg gtattggtta cctcgccgcc cgcgatgacc
     6361 gcgccgctga cctcaaatac cgccgcaatc agcaaagcct gcgggatggt cagcgtaccc
     6421 gcaccgacgc tggtgccgaa agaattggca acatcgttgc cgccgatgtt gaacgccata
     6481 aacacgccga acatcgtggc aataatgaat aaggcggcgt aattgtaatg ggtataaccc
     6541 aagccccaat aaataaaata gccgactgta cctaccagca tggcggcaaa gacggcgttg
     6601 atggttttaa catttgcgct catttggatt tgagccatgg caatcttcct ttaatgtggt
     6661 ggaaatcatg agtgttgccg acactgtact gctgcctccg gtcgaactgc cgtttcggga
     6721 tatggcagac gcatccttcc ttctgaaccg catccgaata cccccaaacc tgaccgaatg
     6781 caaaactgct gccggaaacg gtttgtatga cacttctact actggatgtt gcgggcgcat
     6841 tataatattt ttccatccgt cttgaaacat ttatttacac tttatttaca ctgcggcggc
     6901 aaatcggtat acgagcgtca atacacgtta aaatggcgtt ttgcaccagt ttgggagtga
     6961 tgatggaaac acagctttac atcggcatca tgtcgggaac cagtatggat ggggcggatg
     7021 ccgtgctggt acggatggac ggcggcaaat ggctgggcgc ggaagggcac gcctttaccc
     7081 cctatcccga caggctgcgc cgccaattgc tggatttgca ggacacgggg gcggacgaac
     7141 tgcaccgcag caggattttg tcgcaagaac tcagccgcct gtacgcgcaa accgccgccg
     7201 aactgctgtg cagtcaaaac ctcgcgccgt ccgacattac cgccctcggc tgccacgggc
     7261 aaaccgtccg acacgcgccg gaacacggtt acagcataca gcttgccgat ttgccgctgc
     7321 tggcggaact gactcagatt tttaccgtcg gcgacttccg cagccgcgac cttgcggccg
     7381 gcggacaagg tgcgccgctc gtccccgcct ttcacaaagc cctgttccgc gatgacaaag
     7441 aaacacgcgt ggtactgaac atcggcggga ttgccaacat cagcgtactc ccctccgacg
     7501 cacccgcctt cggcttcgac acagggccgg gcaatatgct gatggacgcg tggacgcagg
     7561 cacactggca gcttccttac gacaaaaacg gtgcaaaggc ggcacaaggc aacatattgc
     7621 cgcaactgct cgacaggctg ctcgcccacc cgtatttcgc acaaccccac cctaaaagca
     7681 cggggcgcga actgtttgcc ctaaattggc tcgaaaccta ccttgacggc ggtgaaaacc
     7741 gatacgacgt attgcggacg ctttcccgtt ttaccgcgca aaccgtttgc gacgccgtct
     7801 cacacgcagc ggcagatgcc cgtcaaatgt acatttgcgg cggcggcatc cgcaatcctg
     7861 ttttaatggc ggatttggcg gaatatttcg gcacacgcgt ttccttacgc agcaccgccg
     7921 aactgaagct cgatccgcaa tgggtggaag cagccgcatt tgcgtggttg gcggcgtgtt
     7981 ggattaatcg cattcccggt agttcgcaca aagcaaccgg cgcatccaaa ccgtgtattc
     8041 tgggcgcggg atattattat tgagcgcaaa tgccgtctga aagcccgttc cgccgttcag
     8101 acggcatttt cgcccctttt gtttacaaac ccttaaaatc catttacact caaac
//
LOCUS       DAWFIT010000089         8142 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFIT010000089 DAWFIT010000000
VERSION     DAWFIT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.089"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..319)
                     /locus_tag="WGQ85_001794"
     CDS             complement(<1..319)
                     /locus_tag="WGQ85_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751164.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEVDESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNT"
     gene            381..491
                     /locus_tag="WGQ85_001795"
                     /pseudo
     CDS             381..491
                     /locus_tag="WGQ85_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            610..1014
                     /locus_tag="WGQ85_001796"
     CDS             610..1014
                     /locus_tag="WGQ85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0751165.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(1049..1924)
                     /gene="rsmI"
                     /locus_tag="WGQ85_001797"
     CDS             complement(1049..1924)
                     /gene="rsmI"
                     /locus_tag="WGQ85_001797"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0751166.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1971..2318
                     /locus_tag="WGQ85_001798"
     CDS             1971..2318
                     /locus_tag="WGQ85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0751167.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            2323..2916
                     /locus_tag="WGQ85_001799"
     CDS             2323..2916
                     /locus_tag="WGQ85_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0751168.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2980..3588
                     /locus_tag="WGQ85_001800"
     CDS             2980..3588
                     /locus_tag="WGQ85_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0751169.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3596..4240
                     /locus_tag="WGQ85_001801"
     CDS             3596..4240
                     /locus_tag="WGQ85_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751170.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            4280..5059
                     /gene="map"
                     /locus_tag="WGQ85_001802"
     CDS             4280..5059
                     /gene="map"
                     /locus_tag="WGQ85_001802"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0751171.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            5214..5513
                     /locus_tag="WGQ85_001803"
     CDS             5214..5513
                     /locus_tag="WGQ85_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0751172.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5751..6125
                     /locus_tag="WGQ85_001804"
     CDS             5751..6125
                     /locus_tag="WGQ85_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0751173.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(6313..7779)
                     /locus_tag="WGQ85_001805"
     CDS             complement(6313..7779)
                     /locus_tag="WGQ85_001805"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0751174.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         2106 a         2414 c         1961 g         1661 t
ORIGIN      
        1 gagtattcgg tactgtaacc gtataaacct taccatttcg cttcaaaaga ccgaatacgg
       61 cgactttacc ggcagcaccg cgaccgcgtt tgcctttgcg ttgtccgcca aaataacttt
      121 catctacttc tacttcgcca tcaaacattt ccaaatgcgg actgttttga taaataagta
      181 atcgtaaacg atgaaaataa taggctgcgg tatttttatt aacgcctact aactctgctg
      241 ccgttcttgc agttacacct gcgacaaaca gttcaatgag tttattttgt ttataccggc
      301 ttagacgact ttttctcata gggataattc taacttaatt tgaatttccc tagttatcta
      361 ggacagcccc taagtttttt atgaacacaa accaacctgc cgtttacgac ccgttgacac
      421 gtacgctgca ctggctgacc gttgccggtt tcatcggcat tccgaccacc attgtcctgt
      481 ggacgattta tagtagatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
      541 aatagtacgg caaggcgagg caacgccgta ctggtttttg ttaatccact atacgaagag
      601 gcggaatggg tgggcagcct gttcggcctg cacaaatctt tcggtttcct tacgctgacg
      661 gtgattacat tgcgcatcgt gtgggcggtt gccaaccgcg ccaagcgtcc gcaaagcgac
      721 agcaaggctg cggcggcagg acacggcatt ctgtatctgc tcatgcttgc cgttcccgtt
      781 atcggcatga tccgccaata cggcagcggc cgcggcccgt tgaaagtgtt cggcgttgaa
      841 gtgatgcagg gttcgccgga aaaaatcgag tggatggcaa acttgggcaa cacgttccac
      901 ggcaatttgg gctggctgct gtttgccgcc gtcgtcggac acgtcgccat ggtcgtcgtc
      961 caccgtgttc aaggcaaaga cgtgctgtac cgcatgacgg ggcgtgtccg ttgattccgt
     1021 tcgcactatg gtgccggtcc gtccggcatc atttgttttt ccaagacagt gccagattgt
     1081 acaaagcttt ttttccctcg cccgtgattt tggcggcaag ctccgccgcc tgtttggtcg
     1141 gcagctcggc tgtgaggatt ttcatgatgt tttgcgcgga ctcggacaag ccttcgtgtt
     1201 tttcatcctg cgccggataa agcaccaaca ccatctcgcc gcgcgattgg ttgccgtcgg
     1261 cagacaatgc cgtctgaatt tccccaaccg tgccgcttaa gaacgtttca aacgttttcg
     1321 taatttcgcg cgccagcatt aatcggcgtt cggggaacag ttccgccata tcggcaagcg
     1381 tcgcaccgat gcggtgcggc gtttcaaaca tgacgatagg aaacgccgcc cgcacccatt
     1441 tggcaaacag tttcctgcgt tctcccgatt tcggcggtac aaaaccgttg aaataaaaat
     1501 cggatccttc cacaccggcc acgctcaaag ccgccatcac cgcgcttgcg cccacgacgg
     1561 gaacgacttt aaacccggcc tcacgcacgc ggccggcgag tttggcaccc gggtcgcaca
     1621 cggccggcgt acccgcatcg gaaacctgtg ccacaaccat gccgtctgaa agatagccga
     1681 caatcttgtc cgccatctgc cgttcgttgt gttcgcgcac actgacaagt ttgccctgaa
     1741 tgccgtacgc gctcaaaagc tgcgcggtaa cgcgcgtgtc ttcggcacag atgatgtccg
     1801 ccttttgcaa taccgccaaa gcgcgcaggg taatgtccgc caaattgccg atgggcgtgg
     1861 caaccacgta taatgtccct ccgacgacgc tgtcggaggc tttctgcaag tgtttctgaa
     1921 acataaaaaa tgccgtctga aaaacagaca ttataaaggt taaaccgatt atgcgcctaa
     1981 accacaaaca gggcgaggca ggggaagatg ccgcgcttgc cttcctccaa tcccaaggct
     2041 gcacactgct tgcccgcaac tggcactgcg cctacggcga aatcgacctg attgtcaaaa
     2101 acggcggcat gattctgttt gttgaagtaa aataccgcaa aaatcggcaa ttcggcggtg
     2161 tcgcatacag catttcccca tccaaattat tgaaactgca acgaagtgta gagtattatc
     2221 tgcaacagaa caggttgaca aacgtaccgt gccgcctcga tgcggtactg attgaaggaa
     2281 accgcccgcc cgagtggata cagaatatta caggttgacg atatgacgac attacaagaa
     2341 cgcgttgccg cccattttgc cgaaagcatc cgtgccaagc aggaagccgg aaaagtattg
     2401 gtcgagccga ccgtacaggc tgccgagctg atgctgcaat gcctgatgaa tgacggcaaa
     2461 atcctggcct gcggcaacgg cggttcggct gccgacgcgc aacacttcgc cgccgaaatg
     2521 accggccgtt ttgaaaaaga acgcatggaa ctcgccgctg tcgcgctgac aacagacact
     2581 tccgcgctga cagccatcgg caacgactac ggtttcgacc acgtattcag caaacaggta
     2641 cgcgccctcg gacgtgcagg cgacgtcttg gtcggcattt ccacttccgg caattccgcc
     2701 aacgtcatcg aagccgtcaa agccgcacac gaacgcgata tgcacgtcat cgccctgacc
     2761 ggccgcgacg gcggcaaaat cgccgccatg ctcaaagaca ccgacgtttt actcaacgtt
     2821 ccctatcctc gaactgcccg tattcaggaa aaccacatcc tgctgataca cgccatgtgc
     2881 gactgtatcg actccgtact gctggaagga atgtaaccct tttcagacgg catggcgcaa
     2941 agcaatgccg tctgaaacgc ccaagaaagg aagcccccga tgaaacccaa accgcacacc
     3001 gtccgcaccc tgattgccgc cattttcagc cttgccctta gcggctgcgt cagcgcagta
     3061 atcggaagcg ccgccgtcgg cgcgaaatcc gccgtcgacc gccgaaccac cggcgcgcaa
     3121 accgacgaca acgttatggc gttgcgtatc gaaaccaccg cccgttccta cctgcaccaa
     3181 aacaaccaaa ccaaaggcta cacgccccaa atctccgtcg tcggctacaa ccgccacctg
     3241 ctgctgctcg gacaagtcgc caccgaaggc gaaaaacagt tcgtcggtca gattgcacgt
     3301 tccgaacagg ccgccgaagg cgtgtacaac tacattaccg tcgcctccct gccgcgcact
     3361 gccggcgaca tcgccggcga cacttggaac acatccaaag tccgcgccac gctgttgggc
     3421 atcagccccg ccacacaggc gcgcgtcaaa atcgttacct acggcaacgt aacctacgtt
     3481 atgggcatcc tcacccccga agaacaggcg cagattaccc aaaaagtcag caccaccgtc
     3541 ggcgtacaaa aagtcatcac cctctaccaa aactacgtcc aacgctgact cggcaatgcc
     3601 gtctgaaccg ccttcagacg gcattgcccg acaccccaaa agcacaatca aaatggcaaa
     3661 aaaaccgaac aaacccttca ggctgacccc caaactcctg atacgcgccg tattgctcat
     3721 ctgtatcacc gccatcggcg cattggcaat aggcatcgtc agcacattca acccgaacgg
     3781 cgacaaaacc cttcaagccg aaccgcaaca caccgacagc ccccgcgaaa ccgaattctg
     3841 gctgccaaac ggcgtagtcg gacaagatgc cgcccaaccc gaacaccacc acgccgcctc
     3901 atccgaaccc gcacagccgg acggcacaga cgaaagcggc agcggactgc cgtcccctgc
     3961 cgcacccaag aaaaaccggg tcaaaccgca acctgccgac acagctcaaa ccgacaggca
     4021 gccggacgac gccggaacac aagctgaaaa cacactcaaa gaaacccccg tactgcccac
     4081 aaacgtcccc cgtcccgaac cccgaaaaga aacacccgaa aaacaggcgc agcccaaaga
     4141 aacgcccaaa gaaacgccca aagaaaacca taccaaaccg gacaccccga aaaacacgcc
     4201 gcccaaaccc cataaagaaa ttctcgacaa cctcttctga cccggcacgg caggcacacc
     4261 cgcaatccaa ggaagcatta tgaacggcat catcatcaaa acccccgaag aaatcgaaaa
     4321 aatgcgcgag ctgggcaaac tcgtcgccga agccctcgac tacatcggac aattcgtcaa
     4381 acccggcgta accaccgacg aaatcgacaa actcgtttac gactaccacg tcaacgtcca
     4441 aggcggctat cccgcccccc tgcactacgg caacccgccc taccccaaat cctgctgcac
     4501 ctccgtcaac cacgtcatct gccacggcat tcccgacgac aagccgctca aagaaggcga
     4561 cattatcaac atcgacctca ccatcaaaaa agacggcttc cacggcgact ccagccgtat
     4621 gtttaccgtc ggcaaagtct cccccatcgc ccaacgcctg atcgacgtaa cccacgcctc
     4681 catgatggcg ggcatagaag ccgtcaaacc cggcgcgaca ctgggcgacg taggttacgc
     4741 ctgccaacag gttgccgaaa acgccggcta ttccgtcgta caggaattct gcggacacgg
     4801 catcgggcgc ggtttccacg aagccccgca agtgttgcac tacggaaaaa aaggacaggg
     4861 ctccgttcta aaaccgggta tgatttttac cgtcgaaccg atgatcaacc aaggcaaacg
     4921 ccacctgcgt atcctcaacg acggctggac ggtggttacc aaagaccgct ccctctccgc
     4981 ccaatgggaa cacgaagtct tggtgaccga aaccggctac gaaatcctca ccgtcagccc
     5041 cgcctccggc aaaccctgaa gccggacgta tccgccccat aaaaacaaac aatgccgtct
     5101 gaaagaaacg gcagatatga tatataatat aaaaacaggc ttgacccggc acattacgaa
     5161 aacaaagcaa atcggaattt gccccgcaac cagacaaact taaaggaagt tttatgaaaa
     5221 tatttgaaaa tatagaagat gttaaagcca tccgtaaaaa gaccgggctg aaccagatag
     5281 acttctgggg caaggtcggc gttacccagt ctggaggctc gcgctacgaa accggccgca
     5341 aaatgcccaa acccgtacgc gaactgctcc gcctcgtcca tatcgaatgc atcgatttgg
     5401 cgaaagtcaa caaaaaagat atggaaatcg ccgccctgtt gaaaaaacac catcccgacc
     5461 tgtatgccga gttgtccaaa cagaccaagt ccgaaagaaa aaaacaaagt taaaccgcaa
     5521 cctccggatg cccgacagtt tttcatttcc gaaaaacgca aacaatgccg tctgaaacac
     5581 cggacaggtc gccgtatccc gcctgccgcc cctgcctcaa accgccgaac cgcccgaacc
     5641 cgccttttta caaactttat ccaatttcct gtttatttca ggatacgccg acattagaat
     5701 gtcaaacagc tcgaaacggg caaactccac atccatccca aggaataaaa atgaaacttc
     5761 tgaccaccgc aatcctgtct tccgcaatcg cgctcagcag tatggctgct gctgccggca
     5821 cgaacaaccc caccgttgcc aaaaaaaccg tcagctacgt ctgccagcaa ggtaaaaaag
     5881 tcaaagtaac ctacggcttc aacaaacagg gtctgaccac atacgcttcc gccgtcatca
     5941 acggcaaacg tgtgcaaatg cctgtcaatt tggataaatc cgacaatgtg gaaacattct
     6001 acggcaaaga aggcggttat gttttgggta ccggcgtgat ggatggcaaa tcttaccgca
     6061 aacagcccat tatgattacc gcacctgaca accaaatcgt cttcaaagac tgttccccac
     6121 gttaatcagg caacaaaaaa cagcgttttc agaaatgaaa acgctgtttt tttgaccgtt
     6181 ccattattcg caaaagggaa aaaacgatta cctgccccgt gtatcaaaac ctgccctgcc
     6241 ggatgaaggg cataaccggc agggacggca tcaacaccat atgggggtac ggcttttctt
     6301 gaaagattcg gcttaaatat ccaacacttt cgcggtatag gcgataattt catccgccct
     6361 ttcggggttt tcgttcaact tgatgccgta acccggtacc agctctttca gacggccttc
     6421 ccaagacggg gcgcgctcgg ggaagcattg gtgcatcagc cggatcatca gcggcacagc
     6481 agtcgatgcg cccggcgacg cgcccagcaa tgcggcgagt gagccgtcgg cgtgggcgac
     6541 aatctccgta ccaaactgga gcacgccgcc tttttcggag tcttttttaa tgatttggac
     6601 gcgttgccct gcggtgatga gttcccagtc gtcggggttt gcctcggggt agtattccag
     6661 cagggaggcg aagcgttctt ctttggtttt acgcaattcg cccagcaggt atttggtcag
     6721 cggcatattc gcccagccgg cgcacagcat aggatagagg ttgtccatat ggatggacag
     6781 cggcaaatcc ataagcgagc cttgcttgag gaagttggaa cggaagcctg cgtaagggcc
     6841 gaacataagg tggcgtttgc cgtccacgtt gcgtgtgtcg aggtgcggga cggacatcgg
     6901 cggcgcgccg acggaagcct gcccgtacac tttggcgttg tgttgttcgg cggtttcagg
     6961 ggtgctgttg cggaagaaca ggccggacac ggggaagccg ccgtagcctt tgccttcggg
     7021 gatgccggat ttttgcagca gggtcagcgc gccgccgccc gcgccgagga agagaaagcg
     7081 ggtacggagg gtgagctgcc cgtcggggtt gcgggtatcg gcggttttga gcacccacgc
     7141 gccgtcggat tcgcgtttga tgtcttcgac gtggcggttg aactcggttt ttacgccctt
     7201 gccctgcaaa tatttcacca tttggcgcgt cagccgtccg aaatcgacat ccgtgccttc
     7261 ggcggagtag ttggcggcga cgggttggtt ttcgtcccgg ccgcgcatca tcagcggagc
     7321 ccaatcggaa attttgttcc gatcggtgga aaattccata ttttcaaaaa gtttttgggt
     7381 tttaaacgcg tcataacgtt tttgaagata agaacaatgg tcttcattca tcaccaaaga
     7441 catatgcggc acggcattga tgaaggaatt gtcttccaac ttgccttccg cgaccaacgt
     7501 cgcccaaaac tggcggctga catgaaactg ttcggcaata ttgagggcgc gcgccggatc
     7561 gataatccca tttgcaccca acggcgcata gttcaattcg cacagcgcgg aatgccccgt
     7621 gccggcgttg ttccacgcgt ttgacgattc caacgccaca tcttccaagc gttcaatcag
     7681 ggtgatttcc caagacggtt cgagttcttt gagcaaaacg cccaaagtcg cgctcataat
     7741 gccgccgccc accaagacaa cgtctgtcgc ttcagccatg gtttactcct aaaaaacagg
     7801 catcttctgc ccttatggtt atttgccgta ctacaaacgc ctgaatcgca aaagcaggga
     7861 aaaccggcaa tggtgtgtgt ccgagtatgc tgtttcaggg ttggaatgcg ttgcaagtat
     7921 ggcttccgac accgcttcag gggcttgtaa tatgttatcg tgaatgtagt ggattttact
     7981 gggaaatgca aagtttttct gtcgcccgcc aagcccggga actgcgaaat gaaaaataaa
     8041 aatagttatt tatctatata tatcaaattt ttaatagata aaaaaccaaa attgtttata
     8101 tattaatttt taaaagattg tcagcatatt gcgttaagtt tt
//
LOCUS       DAWFIT010000090         8132 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFIT010000090 DAWFIT010000000
VERSION     DAWFIT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.090"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(67..291)
                     /locus_tag="WGQ85_001806"
     CDS             complement(67..291)
                     /locus_tag="WGQ85_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0751175.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WGQ85_001807"
     CDS             complement(292..1680)
                     /locus_tag="WGQ85_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0751176.1"
                     /translation="MNAVVVAVIVMLSLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGQ85_001808"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WGQ85_001808"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0751177.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2577..3038)
                     /locus_tag="WGQ85_001809"
     CDS             complement(2577..3038)
                     /locus_tag="WGQ85_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751178.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGQ85_001810"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGQ85_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0751179.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4765..4868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4934..6157
                     /gene="cytX"
                     /locus_tag="WGQ85_001811"
     CDS             4934..6157
                     /gene="cytX"
                     /locus_tag="WGQ85_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0751180.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6154..7254
                     /locus_tag="WGQ85_001812"
     CDS             6154..7254
                     /locus_tag="WGQ85_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0751181.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEDLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7275..7892
                     /gene="thiE"
                     /locus_tag="WGQ85_001813"
     CDS             7275..7892
                     /gene="thiE"
                     /locus_tag="WGQ85_001813"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0751182.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHSDE
                     LKREIARCAAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1749 a         2505 c         2189 g         1689 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact cataatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagtttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 cgcaaacgca ccgagcatcg cataagaaag cgcaatcttc gcgccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcgacagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 gaaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaag tccggcaggg tttccactcc cgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatct gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgtgctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagtgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaagtg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcaattttag gcagttttga caagcccaac cattcgtcaa
     2581 acgtcatttt ttatcccgtc tgccgccgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaag tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagt ccggcatcaa tccgcaaagt cggttgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gaccgttgcc gataatggtc gcgcctttga cgggatattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccgttttc cataaaggta ttggtcatgc gcggcatagg
     3481 ggcggaggcg tagctttcgc ggcggccgtt gccggtcgat tgcgtacccg tcaggcgggc
     3541 gttggtttcg tcctgcatat agccggtcaa aataccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcgccctg
     3661 atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgccg gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac cgatgaacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg ataacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttgtc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcgtaatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatatagtg gattaacaaa aaacagtacg
     4621 gtgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4681 ttttgttaat ccactataga aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg
     4741 ccccctgctt ttgtgtacac ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag
     4801 attgcgaaag cagaatccgt agaacctgtc ggggtaatgc ctgcgtagga aacaaaacct
     4861 ccccgccctt tcgggcttcc gttccctttt ccgcacttcc ccgccccatt ttcatgtttt
     4921 ttaaggactt gatatgtcgg gcaatgcctc ctctccttca tcttcctccg ccatcgggct
     4981 gatttggttc ggcgcggcgg tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc
     5041 tttgggctgg cagcgcggtc tggcggctct acttttgggt catgccgtcg gcggcgcgct
     5101 gttttttgcg gcggcgtata tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg
     5161 cctgtcgttc ggcaaacgcg gttcagtgct gttttccgtg gcgaatatgc tgcaactggc
     5221 cggctggacg gcggtgatga tttacgccgg cgcaacggtc agctccgctt tgggcaaagt
     5281 gttgtgggac ggcgaatctt ttgtctggtg ggcattggca aacggcgcgc tgattgtgct
     5341 gtggctggtt ttcggcgcac gcaaaacagg cgggctgaaa accgtttcga tgctgctgat
     5401 gctgttggcg gttctgtggc tgagtgccga agtcttttcc acggcaggca gcaccgccgc
     5461 acaggtttca gacggcatga gcttcggaac ggcagtcgaa ttgtccgccg tcatgccgct
     5521 ttcctggctg ccgctggccg ccgactacac acgccaagca cgccgcccgt ttgcggcaac
     5581 cctgacggca acgctcgcct atacgctgac gggctgctgg atgtatgcct tgggtttggc
     5641 agcggcgttg ttcaccggag aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg
     5701 tgcggcaggc attttggcgg tcgtcctctc caccgttacc accacttttc tcgatgccta
     5761 ctccgccggc gtaagtgcga acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt
     5821 cggcgttacc ctgatcggca cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa
     5881 cttcctactg cttatcggct cggtatttgc gccgatggcg gcggttttga ttgccgactt
     5941 tttcgtcttg aaacggcgtg aggagattga aggctttgac tttgccggac tggttctgtg
     6001 gcttgcgggc ttcatcctct accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg
     6061 tctgaccgcc cccgtaatgt ctgccgttgc cattgccacc gtatcggtac gccttttctt
     6121 taaaaaaacc caatctttac aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc
     6181 ggcctctcgg gaaggctgac cgcactgcag cttgcagaac aaggttatca gattgcactt
     6241 ttcgataaag gctgccgccg gggcgaacac gccgccgcct atgttgccgc cgccatgctc
     6301 gcgcctgcgg cggaagcggt cgaagccacg cccgaagtgg tcaggctggg caggcagagc
     6361 atcccgcttt ggcgcggcat ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac
     6421 ggcagcctga ttgtgtggca cgggcaggac aagccattat ccagcgagtt cgtccgccat
     6481 ctcaaacgcg gcggcgtagc ggatgacgaa atcgtccgtt ggcacgccga cgacatcgcc
     6541 gaacgcgaac cgcaactcgg cggacgtttt tcagacggca tctacctgcc gaccgaaggc
     6601 cagctcgacg ggcggcaaat attgtctgca cttgccgacg ctttggacga actgaacgtc
     6661 ccttgccatt gggaacacga atgcgtcccc gaagacttgc aagcccaata cgactggctg
     6721 atcgactgcc gcggctacgg cgcaaaaacc gcgtggaacc aatcccccga gcacaccagc
     6781 accctgcgcg gcatacgcgg cgaagtggcg cgggtttaca cacccgaaat cacgctcaac
     6841 cgccccgtgc gtctgctcca tccgcgttat ccgctctaca tcgccccgaa agaaaaccac
     6901 atcttcgtca tcggtgcgac ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt
     6961 tccgggctgg aactcttatc cgcactctat gccgtccacc ccgctttcgg cgaagccgac
     7021 atcctcgaaa tcgccaccgg cctgcgcccc acgctcaacc accacaaccc cgaaatccgt
     7081 tacaaccgcg cccgacgcct gattgaaatc aacggccttt tccgccacgg tttcatgatc
     7141 tcccccgccg taaccgccgc cgccgtcaga ttggcagtgg cactgtttga cggaaaagac
     7201 gcgcccgaac gcgatgaaga aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt
     7261 ctgaaaggaa ccccatgacc ttcctgccac tcaaatcccc gctcaaattc tacgccgtcg
     7321 tccccaccgc cgattgggtc gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc
     7381 gctgcaaggc cctgcacagc gatgaattga aacgcgaaat cgcccgctgc gccgcagcct
     7441 gtcagggcag ccatacgcag cttttcatca atgaccactg gcgcgaggca atcgaagcag
     7501 gcgcgtacgg cgtgcatctc ggacaagaag acatggacac cgccgacctt gccgccatcg
     7561 ccgccgccgg tttgcgcttg ggtttgagta cgcactccgt tgccgaactc gaccgcgccc
     7621 tgtccgtaca ccctagctac atcgccagcg gcgcgatttt cccgaccacg accaagcaaa
     7681 tgcccaccgc cccgcaaggc ttggacaaac tgcgcgaata cgtcaaacag gcaggcggca
     7741 cgcccgtcgt cgccattggc ggcatcgatt tgaacaacgc ccgagccgta ctcgccaccg
     7801 gcgtttcctc actcgccgcc gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg
     7861 ttaaagcgtt tcaggctttg tgggatggat aaaaccgaaa gaagaaaatt caattgccgt
     7921 gtcggcaaaa cttagcccgt tatcgcaaat atgcttaact ttaaatgtgg catatcatca
     7981 aattccgtca ttcccgcgca agcgggaatc cgccttaaaa cttgagaaac catcatttga
     8041 aaaacagttt ccgaatttca aaaatggatt cccgcctgcg cgggaatgac ggcaaccggc
     8101 cggttgcgta tcaaaaaata aagtaattcg gc
//
LOCUS       DAWFIT010000091         8088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFIT010000091 DAWFIT010000000
VERSION     DAWFIT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.091"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..767)
                     /locus_tag="WGQ85_001814"
                     /pseudo
     CDS             complement(1..767)
                     /locus_tag="WGQ85_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(931..1563)
                     /locus_tag="WGQ85_001815"
     CDS             complement(931..1563)
                     /locus_tag="WGQ85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751183.1"
                     /translation="MEKIEKFKSELLNAIFQYTQCISIFVYKKNIYYLIDCKENFILN
                     MKLDLDLDFKNGNITLEQYQDEMNSYYYRNGIWQLTKDNFESYLQSDSVIELKKDELK
                     KLMFQGFTSAEAVRLYSVVENKLSYNDPISDSGQQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(1718..2212)
                     /locus_tag="WGQ85_001816"
     CDS             complement(1718..2212)
                     /locus_tag="WGQ85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ0751184.1"
                     /translation="MIFKQNQNYWAVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTKR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLDAWDAIDADDVILSLDNSPEEIGTGLRLAL
                     SRCR"
     gene            2536..3969
                     /locus_tag="WGQ85_001817"
     CDS             2536..3969
                     /locus_tag="WGQ85_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0751185.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            4078..4866
                     /locus_tag="WGQ85_001818"
     CDS             4078..4866
                     /locus_tag="WGQ85_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0751186.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            5234..6790
                     /locus_tag="WGQ85_001819"
     CDS             5234..6790
                     /locus_tag="WGQ85_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0751187.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVRITRLSEE"
     gene            6804..7550
                     /gene="surE"
                     /locus_tag="WGQ85_001820"
     CDS             6804..7550
                     /gene="surE"
                     /locus_tag="WGQ85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0751188.1"
                     /translation="MNVLISNDDGYLSEGIDVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            7806..>8088
                     /locus_tag="WGQ85_001821"
     CDS             7806..>8088
                     /locus_tag="WGQ85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0751189.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT         1923 a         1939 c         2135 g         2091 t
ORIGIN      
        1 tgccgtctaa caactgatgt tcttccagat gttcggtttt ccgcactgtc atagcctttg
       61 tcggcataga tggtcgtgcc ttcggtaatg ccttccagca aaggcaacag gtggttgcac
      121 tcatgggtat tggctggggt gatgtgcagt ttctcgatat agccttccgc atcggtacgg
      181 gtatgttgtt tgtaaccgag tctgtagagg ccgtttttct ttgtccaacg ggcatcgctg
      241 tctttactcg gtgtggtttg gccgctgact tgtccttctt catcgacttc tatggcctga
      301 cgctgtttgc tgccggcggt ctgaataatg gtggcgtcaa tgacggcggc ggatgctttc
      361 tctattttta ggtttttttc ggtcagttgg cggttaatca gttccagcaa ttcggacagg
      421 gtattgtctt gcgccagccg gttgcggtag cggcataagg tgctgtaatc ggggatgctc
      481 agttcgtcaa aacggcaaaa caggttgaaa tcgatgcggg tgatgaggct gtgttcgagt
      541 tcgggatcgg agaggctgtg ccattgtccg agcaggacgg ctttgaacat ggacaacagc
      601 gggtaggcgg gacggccgcg gtggtctcgg acgtaacgga ttctttgacg gttcaggtat
      661 tgttcgatcg gttgccaatc aatcacttga tctagcttca atagtgggaa gcggtcgatg
      721 tgtttggcaa tcatggcttg tgcggtttgc tgaaagaagg tgctcatgga aaatccccta
      781 aatgtcttgg tgggaattta ggggattttg gggaattttg caaaggtctc ggcctgagtg
      841 ttgttttaca ggaaagtaaa ggccgtctga aagggtttac cagctttcag acggcctttt
      901 gaattcatgc aagctatggc ttgcttatat ttataattgt ctaaatttcc aataatcccg
      961 tccttcaatt ttccaataac aaaattcgtc cggtattaaa tacccaaaat ccatcgcctt
     1021 tgaaaaccag ccatcataaa cataatcttc atgacatctg tcccaatcca tatgtaagta
     1081 cacttcagtg tcaaaattga catagaataa aggcagcctc gaggaaatct gattgatttt
     1141 taagaaatca ctttgctgtc cagagtctga gataggatca ttgtacgaca gcttgttctc
     1201 gacaacggaa tataacctta ccgcttcagc agaagtaaat ccctgaaaca tcagtttctt
     1261 caattcatcc ttttttaatt caatgactga atctgattga agatagcttt caaaattgtc
     1321 cttagttaac tgccagatgc cgtttcggta ataataacta ttcatttcat cctgatattg
     1381 ttctaacgta atattaccgt ttttaaaatc taaatctaag tctaatttca tgtttaatat
     1441 gaaattttcc ttacaatcaa tcaaataata tatatttttc ttatagacaa aaatagatat
     1501 gcattgagta tattgaaaga tggcatttaa taattcagat ttaaatttct caattttttc
     1561 catatttgcc aaattcctta atagctttat cttcagcaag tcgtagcccg cattaaatct
     1621 gcaggtaagg tgtggtacgc acgccagtga gcttgagtct aatgaaaaag gtcgtctgaa
     1681 acatttttcc ggctttcaga cgaccttttg tcaaatatta tcggcagcgg ctcaatgcta
     1741 accttaaacc tgttccgatt tcttcaggac tgttatccaa tgataaaatt acatcgtctg
     1801 catcaatggc atcccacgcg tccagcttga catggcggct cgggctgatt ttcaggcagc
     1861 cgttgtgcag ccaaatatcc acgctcatca tgtttttaaa tagggcgcgt ttggttttat
     1921 agcccaagtt cccgcatagc ttggcaaccc aatcctcata gcgttgccga attttttcgg
     1981 tatcaaaaaa atcttggtct tctggactgt cataaacgaa agtcctgctg tttgccaatg
     2041 cttgcaagac cgttgtgcct aaagtttcat tgtcggtatc caatggcagg atatgagggg
     2101 gatataggtg gtctattgcc gttaatccca aacctgaaca tgtttgaaca atcagagctt
     2161 cttcattcgt ataaaaaact gcccaataat tttgattctg tttaaaaatc attattttat
     2221 ctccgtaatt ttgactgtaa tgttttgact tttgccatac tctaccacac gttgcaactg
     2281 caatctttgt tccttatata ttatcaaagg gctgcattac ttttctttct aaattaccat
     2341 tgtataaaaa gtatttttct taaacgacag ggattgcgcc cgcgccatat tcagacaccg
     2401 cccggatgtt gcactgcccg atcggatgct tcagacggca tcggaagggt ttgcagtttt
     2461 ggaatatgaa gatgataatg tccgcgagat tgacggcatt tgaaagcggt tattccgcat
     2521 accggaggat acgaaatgaa cgaatattac caattaatca agcatcccga tatttccctt
     2581 tccccgattt cagacggcat cggggtcggc aatccggcaa cgggcgaaat tttggcgtat
     2641 gtccgcaata cgggttcgga caaactgaaa aacctgattc aaaaggcggc ggcagcgcaa
     2701 aaattatggg cggcgaaaac tgctttggaa cgcgccgaca tattgtggcg ttggtatttt
     2761 ttagttaaag aaaacaaaga agcactggcg cgcctgatga cgatggagca gggcaaaagc
     2821 ctgaccgagt cgcgcggcga aattgattat gcggcttcgt ttgtgcgctg gtttgccgaa
     2881 gaggcgcggc ggattgacgg cgatgtgtta acgagtgtaa aagcgtcgca aaaactggtt
     2941 gtgttgaaac agcctgtcgg cgttaccgcc gcgattacgc cgtggaactt cccatctgca
     3001 atgattgcgc gcaaggctgc gcctgctttg gcggtgggtt gcgcgatgat catcaaaccc
     3061 gcatcgctca cgcctttgag tgcgtatgcc ttggcttcgt tggcttacga agcgggcata
     3121 ccgcaggatt tgttgcccgt cgtcagcggc cgcgcatcgg aaatcggccg tgaatttgcc
     3181 acgaacccga ttatccgcaa aatcagcttc accggttcga ccgaagtcgg cgcgaaaatt
     3241 tttgccgaca gcgcggcgga cattaaaaaa ctcagtttgg aactgggcgg caacgcgccg
     3301 tttatcgtgt ttgacgatgc cgatttggac aaagccgtcg aaggtgcgct cgccagcaag
     3361 ttccgcaaca gcggtcagac ctgcgtctgc accaaccgcg tttacgccca atccggcatt
     3421 tacgacgaat tttgtcgcaa attgagtgaa aaagtagccg agctcaaatt gggcaacggc
     3481 ttggaggatg gcgtcaacca agggccgctg attgaggaaa aagcggtaga aaaagtcgag
     3541 cagcacattg ctgacgcgct cgctaaaggc gcggtctgtc tgaccggcgg caaacgcagc
     3601 gcattgggcg gaacgttttt tgagccgacc gtcttaagcg gcgtaacggc gcaaatggcg
     3661 gtggctcgcg aagaaacctt cgggcctttg tgtccggtat tccgttttga aaccgaagcc
     3721 gaagtcattg aggctgcgaa caatacggaa tacggtttgg ctgcttacct ttttaccgcc
     3781 gacaccgcac gccagtggcg cgtcggcgaa gcattggaat acggtatggt cggcatcaat
     3841 acgggcttaa tcagtaatga agcggcaccg ttcggcggcg tgaaacgcag cggtttggga
     3901 cgtgaaggca gcaaatacgg tgcggacgaa tatctggaat tgaaatatct gtgtatggat
     3961 gtcgggtgac ggatgccgtc tgaacggcag ggtttcagac ggcgccgcat tttaagcagt
     4021 ctctatctgt tgtacaatgc gccctgtttt tacggttatt tttgatttga acaagatatg
     4081 atggaaaacg gaaaaacagt cccgagatgg cgttttgcct tgaaaagtgc gggctggcac
     4141 ctcttaatca gcctgtcggt tgcagggctg gcggcattgc tggtttttaa ggtttggtat
     4201 ccttatcctt atgccgagct gacgggaggg ctgtcgcttt atcagctggt ggtggctgtc
     4261 gatattgtat gtggtccgct gctgacttta attttggcaa gcccgaagaa aaagacaaag
     4321 gcacgcatgg tcgatttttc catggtcggc atcatccagc tggcggcttt ggtgtacggt
     4381 ctgcacagcg tttctttggc gcgccctgtg atagaggcgt ttgaaaaaga caggattaat
     4441 gttgtgaccg ccgccgaagt ggcggttgaa gatttgcaca aagcgccgga agggctgcaa
     4501 agcctgtcgt ggttcggcat ccgccgcatt gcattgaaag aacctgagga tgcggatgag
     4561 aagaacaaga cgctggattt gtccctgaaa ggtatcgagc cgagtatgcg tcccgaccgg
     4621 tggctgccgt attccgacaa ggaagcagaa aaaatccgca aacatctgaa accgctgaaa
     4681 gtcttggcgg atgcgagaaa aacgacggtt gcggacattc tgaaacaggc aggtctcgcc
     4741 gaaggggagg agctgtatta cctgccgttt accagcagca ggcagaaaga gtggatagtc
     4801 attaccgata aagagggcaa caccaaaggc tacgcgccga tagacggctt catcatcacc
     4861 ccttaagcgt tgggactccg tccgcactcg aacatccgtt cttcgcggcg gtagaatcag
     4921 actgtatttg agaggggcgc aacccctcaa tcagggcaac acatagggga aggctttatg
     4981 tgccgtccgt gtgttgaaac atttcaaaat aagctcatgc cgtccgaaca tcctttcaga
     5041 cggtatggcg ttttgccatt gccccgataa gctgttaaac tatttaaatt atttcgaccg
     5101 aaggtacacg cacatgcaag accccgagca gagcagcaag cccgcccgcc gttttttgtg
     5161 cgtaccgtct gttcaggcag cttgagcgct tcaggctgcc ttttcgcgtt tgaacttgag
     5221 gaatacgaaa atcatggatt tcagttggtt ggcagaaccg catacctgga taggttttgc
     5281 cacgcttttg gtgttggaag tcgtattggg gatagacaat cttgtctttg tggcgatttt
     5341 ggcaaacaag gtccagcccg cacggcgcga ccgcgcacgg attatcgggc tggggctggc
     5401 agtcgtcatc cgcatcatta tgcttgcttt tatggcgcac atcatcacgc tgaccgagcc
     5461 gctgttccaa atcggcggcc tcgccgtttc cggcaaggac atgattatgc tcgcgggcgg
     5521 tattttcctg ctttacaaag ccaccaccga actgcatgaa cgcctcgaag ggcacaaccg
     5581 ttttaccgtt gccgacaacc aaaaaaaaca cgcgccgttt tggggcgtgg tcgcgcaaat
     5641 cctgatactg gatgccgtgt tttccatcga ttcggtcatt actgcggtgg cgatggtcga
     5701 tcatatcgtc gtggcgatgg gtgcggtcgt cgtcgcgatg gctgtaatga tttctgccag
     5761 caaactcttg accgaatttg tcgacagaca ccctaccgtc gtgatgctct gccttggttt
     5821 tttgttgatg atcggtttca gcctgattgc cgaagccttc catttccaca ttcccaaagg
     5881 ctacctctac gccgccatcg gcttctcgat tttaatcgaa ctgtttaacc agatttccca
     5941 gcgcaacagc cgcaaaaacg actacatcgg cagctcgtgg cgcaagcgca ccgccgaaaa
     6001 cgttttgggt atgatgggca tacgcgaaag cgtgcttgcc gatgcgggcg gcgaatccgg
     6061 ggacgacgcg cattttgaag aaaacgaaaa atcgatgata cgcagcgtgc tgacgcttgc
     6121 cgaacgcccg attatggggg tgatgatccc acgccgcgac atcgaacggc tggacatttc
     6181 ccaaagccgc gaagaacagt gtgcccaact gcaaaacacg ccttacagcc gcctgctcgt
     6241 tgtcggtaag gcgggcgtgg acgaaccttt gggctacatc aacaaaaaag acctgctgtc
     6301 ccaactgctg gaaacaggcg gtctcgacat tcagacggca ttgcgccagc cactcgtcct
     6361 gcccgacagc accaccgcgc tgggcgcaat cgaactcttc cgccaaagca gcgcggatta
     6421 tgctttggtg gtggacgagt tcggcgcggt attgggcatg gtaaccatga aagacctgct
     6481 cgaaaccatc gcaggcgagt tccccgaaga atttgagcgc gaagaagaac cagccgttca
     6541 ggggaatccc gatgaaagcc tgacggtgga aggcgcgttg gaatatgtgg aactcgcacc
     6601 gcaactcaac ctgccgcagc aggaggaaga tgccgatttc catacggttg ccgggctgat
     6661 tatggaagaa ttgcaaacca tccccgatgt cggtgatttt gccgatttcc acggctggcg
     6721 gtttgaagtg gtcgaaaaag aagggcagcg catcgaacgg gtcaggatta ccaggctgtc
     6781 cgaagaatga ttcaggataa gaaatgaatg ttttgatttc caacgacgat ggctatcttt
     6841 ccgaaggtat cgacgttttg gcgcgcgtta cggcggaatt tgccaacgtc agagtggttg
     6901 cgcccgaacg cgacaggagc ggggtcagca attcgctgac gctggaacgc cctttgcagt
     6961 tgaaacaggc gcaaaacggg ttctactatg tcaacggcac gccgaccgac tgtatccaca
     7021 tcgggcagtc tgtattttcg gattttcagg ccgattttgt cttttcgggc attaaccggg
     7081 gcgcgaatat gggggacgac acgctttatt cggggacggt tgcggcggca accgaagcct
     7141 accttatggg catacccgcc gtggcgtttt ccttaaacga cgcttccgga cgctattggg
     7201 cgaccgcaga acaggcactg tggacattgt tggcgcattt tttcaaaaac cccccgcagt
     7261 cccctatttt gtggaacatc aatatccccg ccgttgcgcc ggaagatgtg cggggcatta
     7321 aaatcgcccg tttgggcagg aggcatcacg gtcagaacgt cattcccgcg cgcaatccgc
     7381 gcggcgaaca gatttattgg ataggaccgg tcggcgaagt ttccgatcgg gaagagggaa
     7441 cggatttcgg tgaatgcggc gcaggtttca ttaccgtaac gccgctgcaa atcgacctga
     7501 ccgcctatcc ggacatggcg gaaacagcgg cgttctggca tgcggactga ccgtttcatc
     7561 aaatatagtg gattaacaaa aaccagtacg gcgttgcctc gccttagctc aaagagaacg
     7621 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg
     7681 cttcgtcgcc ttgtcctgat ttttgttaat ccactatacc gtcgggcttt gcgcttgaga
     7741 agaacgcgca tttttattat gatataccgc attcaggcta tatatcggaa catgaaggtt
     7801 atcttatgtt gaaacaaacg acacttttgg cggcttgcgc ctcctttgcc gcactgttgg
     7861 gcggttgcgc cacccaacag cctgctcctg tcattgcagg caattcaggt atgcaggacg
     7921 cgccgtcttc ggcagtttac aacaacccct atggagcaac gccgtacagc ccggctcctg
     7981 ccggcgatgc gccttatgtg ccgccggtgc aaagcgcgcc ggtttatacg cctcctgctt
     8041 atgttccgcc gtctgcacct gccgtttcgg gtacatacgt tccttctt
//
LOCUS       DAWFIT010000092         8011 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFIT010000092 DAWFIT010000000
VERSION     DAWFIT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.092"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            282..1409
                     /locus_tag="WGQ85_001822"
     CDS             282..1409
                     /locus_tag="WGQ85_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0751190.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1439..2316
                     /locus_tag="WGQ85_001823"
                     /pseudo
     CDS             1439..2316
                     /locus_tag="WGQ85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            2320..2508
                     /locus_tag="WGQ85_001824"
     CDS             2320..2508
                     /locus_tag="WGQ85_001824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0751191.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(2585..4096)
                     /gene="lysS"
                     /locus_tag="WGQ85_001825"
     CDS             complement(2585..4096)
                     /gene="lysS"
                     /locus_tag="WGQ85_001825"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0751192.1"
                     /translation="MSEQNHPQTEPQLDENQIITLRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4093..4245)
                     /locus_tag="WGQ85_001826"
     CDS             complement(4093..4245)
                     /locus_tag="WGQ85_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751193.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(4251..5504)
                     /locus_tag="WGQ85_001827"
     CDS             complement(4251..5504)
                     /locus_tag="WGQ85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0751194.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            5962..7758
                     /locus_tag="WGQ85_001828"
     CDS             5962..7758
                     /locus_tag="WGQ85_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0751195.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1997 a         2241 c         2031 g         1742 t
ORIGIN      
        1 atagtgtata ttaagtacgt ctgatatacg cgataccctc cgagggtgta aactttagtt
       61 cacatttaaa atgacctctt taaacctgcc tttcggcagg tttcttttta ggttgtttgg
      121 aaatcgtgtg cagacaaggt gtaaaatagg taacaacata aaataatgcg gttttaccgc
      181 ccatatattt acaaaagcca aatttttaaa catatatcct tgatatatac acggcgtaaa
      241 tatatactgg aaacatcttt aaattttccg aaattttaaa tatgagcaac tggaaacccg
      301 atattcccta taacgattta ccacccctgc cgccaaaaca ggatattgaa agcaaaacca
      361 tcctgaaacg ttgtatagcc gcccgtgcat cccttgcccg tttaaagcag gcggcagaat
      421 tgataccgaa tcaagccatg ctgattaaca cccttcctgt tatggaagcc cgtgcaagtt
      481 cggaaattga aaacatcgta accacaacgg acaagctgtt tcaatccctg caaatggata
      541 cggaacggca agaccctgcc acgaaagaag ccctgcaata ccgcaccgcc ctgtttgcag
      601 gctatgaatc actgacgagc cgccctttat gcacacaaac cgccatcatg gtctgcaacg
      661 ccatcaagca cccctacgaa atggccatcc gcaaaacagg cggcacagcc ctaaaaggag
      721 gcaacagcgg aaatgttgtc tataccccgc ccgaaggaga agaaaccata cgcggcaagc
      781 tggcaaattg ggagcggttt attcacgaaa gcggcgattt agacccgctt atcatcatgg
      841 cggcggcaca ttaccaattt gaagccatcc atccgtttac ggacggcaac gggcggacgg
      901 ggcgcatatt gaacagcctg ctattgattg aaaaagggct tttggatttg cctattttgt
      961 atttgagccg ctacatcatc gaaaacaggg cggactatta ccgcctgctt ttaggcgtaa
     1021 ccgaacggca ggattgggaa agctggataa tctacatctt agacggcgta gccgataccg
     1081 ccgattggac ggtatcgaaa atagatgcga tacgccgcct gttcgagcag acacggcaac
     1141 acatacggac acacgcacaa ggaatctaca cgcacgaact ggtaaatctt ctgtttgagc
     1201 agccatatac acgcattgcc aacctagaag cggcagggat agccaaacgg cagacggcct
     1261 ctaagtacct gaaagagctt tcagacatag gtgtgctgca agaaatcgtc atcggcaggg
     1321 acaaactatt cattcatccg cgcctaatgg aactattgcg gggagagggc aacagcttta
     1381 cctcattcca atccctcgtt aaagcatagc caaaataatc aataatccgg aggtcaatat
     1441 ggcaagaagg tcaaaaacat ttgaagaagc tgctgctgag gttgaggaac gtttcggtca
     1501 tcgtggcatt aagttggtcg agtttgaggg tacagccaag ccgtgtgtaa tcaactgccc
     1561 taaacatgga aaccaaacct gttcgaggta ctccaatatg ttcataggaa gtagctgggg
     1621 ttgcccctct tgtggtaatg agcaagctgc aaaagccggt atagcgaccc ttaggaagaa
     1681 tcacatagcg ttagaaatgc tgaaacaggc tgtaacaggt atgaccaagc aagagcgcat
     1741 cacgacgcaa gcctacaatg agatgaccaa atccgtggca ggttcaaaca gcatagtcct
     1801 taacgatgtc caaggcgata cgaccatcaa caaccatcat acgcatacgc acaaccacag
     1861 cgatgccgac ggcaaagcac tgtcgatgag gctcacacca cgccccctgt tgtcagaccg
     1921 tcaggcggcg gctttcgccc gtacaggcaa actcacaggc agtttcgact tgtttgcttc
     1981 ggtggtcgcc ccctcgcagt acacgtttgc cgttgccatg cccgacacgt ccatgtcgcc
     2041 ggttatcgaa aaaggggatt tggtggtggt cgagccgcgt atgcgccctg cggacgagga
     2101 catcgtgctg attgaactgt ccgacaagcg gctggtcgtc gcgcaccttg ttatcgatat
     2161 tgcgggcagg atgctgattt atcagacggg caggccgtct gaagcctttg acctgcccga
     2221 aggcagcacg attttaggtg tggtgctgga gtcaaaaaac ggtttatgtc cgccgcacag
     2281 gcaagaaggc gtgttgattc ggattaccac ccctgatgtg tggacggttg gtacgatttc
     2341 cgcttccaaa acgtcgtgta cgcgcccgac cgcagcccgg aaatcagccg tatgctttct
     2401 tcgattttgg caggctacgc gtgggatacc gaaaacccgt tcgtggcgaa atccgaacaa
     2461 cgcctgactg ccttgtccga atgggtcggt cagttagaaa gcgaataaat ccgtaccgcc
     2521 atacaaaatg ccgtctgaat ccaatcgggt tcagacggca ttgccatttc aactgttttt
     2581 atgactactc ggggcgcatc tgcggaaaca gaatcacgtc gcggatggtt tgcgaatcgg
     2641 tcagcaacat gaccaagcgg tcgatgccga tgccgcagcc gccggtcggc ggcaggccga
     2701 attccatcgc gcggatgtag tcggcatcgt agtgcatcgc ttcgtcgtcg cccgcgtctt
     2761 tttgcgccac ttgtgccttg aagcgttcgg cttggtcttc cggatcgttc aactcggagt
     2821 agccgtttgc cagttcgcgg ccgacaacga aaagctcgaa gcgttcggtc aagcccggtt
     2881 tgctgtcgga tgcgcgtgcc aacggggaca cttcgacagg gtagtcgata atgaaagtcg
     2941 ggttccacaa tttgccctcg gcgcaacctt caaacagggc aagttgcagg ctgccgatgc
     3001 ccggagacgg aggcaggctt tcgccgtgtt tgacgatttc ttttttcagc cactccgcat
     3061 cgttcaactg ctcgtcggtg tagtgcggat tgtatttttt gatggcatcg agaatggtca
     3121 gacgctcgaa cgggctttcc aaatcgactt ctttgccgtt gtaagtgatg tttgccgtgc
     3181 cgtttaccgc gcgggaagcg ttgcggatga tgtcttccgc catttgcatc atgcgttcgt
     3241 agtcggagaa ggcttcgtag aattcgatca tggtgaattc agggttatgg cgcacagaca
     3301 taccttcgtt gcggaagctg cggttgattt caaacacgcg ttccaaaccg ccgacaacca
     3361 agcgtttcag atacagctcg ggggcgatgc gcaggtagag cggaatgtcc aaggcattgt
     3421 ggtgggtaac gaagggtttt gccgtcgcgc cgccgggaat cgggtgcatc atcggggttt
     3481 cgacttcgag ataatgctcg cccaccataa aattacgcac ggattggatg atttggctgc
     3541 gtttgataaa ggtattgcgc gattcttcgt tggcaatcaa atcgacatag cgttggcggt
     3601 atttggtttc ctgatcgctc aagcctttat gtttgtccgg cagagggcgc agggatttgg
     3661 acagcaggcg gatgccggac acgcgtacgg tcagttcgcc ctggttggtt ttgaacaaag
     3721 tgccttccgc gccgacgatg tcgcccaaat cccaatggtt gaagtcgtcc aaaacttctt
     3781 ggctcacgcc tttgttgttc agataaagct ggatttgccc ggacacgtct tgaatagtgg
     3841 caaagctcgc cttgcccatt tgacgcttca gcatcatgcg gccggccact ttgacgggaa
     3901 tgccttgcgg atcgagttct tctttgccga tttcgccgta ttgggcgtgc aaatcggcgg
     3961 cgaagctgtc gcgtttgaag tcgttgggat aggcgttgcg ctgttggcgg atgttgtgca
     4021 gtttttcgcg gcgcagggtg atgatttggt tttcgtccaa ctgcggctcg gtttgcggat
     4081 ggttttgttc gctcataggg ttttccgaaa aaataaatca ggcgcaatct gtttcagacg
     4141 acctgaccga atcacaaaat ttgcgcgtat tttacgcgat gtcggcattt ttttccataa
     4201 acgcggcaat gccgtctgaa agcggtttgc ggtttcagac ggcatcgtta tcatttgaac
     4261 attcccgcca aattcaataa gaacaaaacg gtaaaaccgg tcagataaat caagcctgcc
     4321 aatgcaaggg cattcatacc tgatgtgagt ttgtgttttt catcaccttt gaccaaacgg
     4381 taattcagcc aggcaaacac aggagcggac acaaaagcgg caatcatcgc aaatttgagc
     4441 agattcgcca ttacgccgtc aaaccaaaaa atcaccgcca aaccgctgcc cgccacccaa
     4501 atattccagg caaagaattc ggcgttgccc gttttgtctt ttccgcgcag caggcgcacg
     4561 ggttcggcaa tggcacgggc atagccgtcc acgacggtaa tcgtcgtgcc gtacatacag
     4621 gcaaacgcga taaacgccac cagcgggcgc gaccagccgc cgatggtaac ggcgtacata
     4681 ttgatcaatt gcccgatata tttgccgccc gccatctgta ctgcttcgcc gttgccgtat
     4741 tgcacaaacg cacccagtgc aaggaaaacc aaagccaaaa ccgcactggc gatataaccg
     4801 acgttgaaat caaaaatccc gtcgcggtat tcggaaggat tgatgcgttg tttttcggtt
     4861 acccacaaag aattgatggc ggaaatttca atcggcgcgg gcatccagcc catcagcgcg
     4921 atcaggaagc ccaaaccggc aagcgtccac ggtgtcggct cgataaaatc ggactgcatc
     4981 tgcataccgc gcgacatagc gatgccggcg gcggcaagcg tggcgatact caaagtaacg
     5041 atgatgattt tggaaacgcg atccaaagcg cggtaacgtc cgctcaccaa aataatcagg
     5101 caggatgcca taatcaaggc ggcaaccgtg ccggcatcaa acatcagcga gggaatcgcc
     5161 attttgacga tggcggcggt tacaatggcg accgcgcccg cgttaatcgt ggcggagagg
     5221 atgcacaaaa tcaggaatac ccacaaataa acgcggcttt tctcggcata accttcaatc
     5281 aggctcttgc ccgtgtccag cgtgtaatgc gcgctgaagc ggaaaaacgg gtatttgaag
     5341 aggttggtca ggatgatgat gagcgcgatc tgccagccgt aaagcgcgcc cgcctgcgtc
     5401 gaggcaatca ggtgcgaacc gccgaccgcc gccgaagcca tcataatccc cggtcccaat
     5461 gcgttgattt tacttttcca agtcgaaata tgttgttcgg acataaagtc ttccgtattt
     5521 ttaaccgtgt ttcaacacac agagccgcat attcggacac agccctatct attgctccaa
     5581 tttgggcggg attgccccca aacaaaccca aatcctaccg tcttcaaaaa caggataccg
     5641 cccggtaggg aaattttgat gaaagcgcgt attgtaacgt aatccaaata cctgccaaca
     5701 cacactatta gaacttcatg ctcaaacttg actatatttt ccgtattact tccaaaaaaa
     5761 tgcataaaac gatatcttat gcctaaaatt ttacaacaaa caaccttaca tcgctttttt
     5821 cgcgcaaaca cgcaccatcc gatcagcccg tccgttttgc agcaggctgg cgatttgata
     5881 agatggttat gtttttcaga cggcatttca gatttccgtc catgccgtct gaagccgcaa
     5941 aacccgattg gaggaactgt tatgaatacc gtatcgaatt acctgtccgc attgcgcgaa
     6001 gccatgaagg cgcaaggctt ggatgcactc gtcatccctt ccgccgaccc ccacctgtcc
     6061 gaatacctgc ccgagcattg gcaggcgcgc cgcgaattat cgggctttac cggctcggtc
     6121 ggcacgtttg tcctgaccac cgatgaagcg ggcgtatggg tggacagccg ctattgggaa
     6181 caagccgcca agcagcttgc gggcagcggc atcgaactgc aaaaaagcgg gcaagtgccg
     6241 ccgtataacg aatggctcgc ggcaaacctg cccgaaaacg ccgccgtcgg catcccttcc
     6301 gatatggtct cgctcaccgg caaacgcact ttggcgcaat ccctgaccgc caaaaacatc
     6361 cgcatcgaac acccggacga tctgcttgac caagtgtgga caagccgtcc cgctatcccc
     6421 gccgaaaccg tgttcgtcca cgaccccgcc tacgtctccg aaaccgccgc cgaaaaactc
     6481 gcccgcgtgc gcgccgtgat ggcggaaaaa ggcgcggatt accacttggt ttcctcgctc
     6541 gacgacatcg cctggctgac caacctgcgc ggcagcgacg tgcctttcaa tcccgttttc
     6601 gtgtcctatc tattgattgg caaagacaac gccgtcctgt ttaccgaccg atgccgtctg
     6661 aatgccgaag ccgccgccgc gctgcaaacc gcaggcatca cggtcgagcc ttacgcccaa
     6721 gttgccgaca aactcgcgca aatcggcggc gcgttgctta tcgaaccgaa caaaaccgcc
     6781 gtcagcacgc ttgtgcgcct gcccgaaagc gtgcgcctta tcgagggaat caacccgtcc
     6841 acgctgttca aatcctgcaa atccgaagcc gacatcgccc gcatccgcga agcgatggaa
     6901 cacgacggcg cggcgttgtg cggtttcttc gccgagtttg aagacatcat cggcaacggc
     6961 ggcagcctga ccgaaatcga cgtggacacc atgctttatc gccaccgcag cgtgcgccca
     7021 ggcttcattt cattgagttt cgacaccatc gcaggcttca acgccaacgg cgcactgccg
     7081 cattacagcg caacgcccga aagccacagc accatcagcg gcaacgggct tttgctcatc
     7141 gactccggcg cgcaatacaa aggcggcacg accgacatca cccgcgtcgt ccctgtcggc
     7201 acacccaccg ccgaacaaaa acgcgacaac accctcgttc tcaaagccca tatcgcgctt
     7261 gccgaagccg tgttccccga aaacatcccc tcgccgctga ttgatgcgat ttgccgcaaa
     7321 cccctgtggc aggcgcaatg cgactacggc cacggcaccg gacacggcgt aggctatttc
     7381 ctcaacgtcc acgaaggccc gcagcgcatc gccttcgccg cccccgccac gcccgaaacc
     7441 gccatgaaaa aaggcatggt tacctccatc gaacccggac tctaccgccc gggaaaatgg
     7501 ggcatccgca ttgaaaacct tgccgccaac caagccgtcg ccgcccctca agaaaccgaa
     7561 ttcggcagct tcctctgttt tgaaaccctg accctctgcc ccatcgacac ccgcctgatg
     7621 gacaccgccc tcatgaccga cggcgaaatc gactgggtca accgctacca cgccgaagtc
     7681 cgccgccgcc tcgagccgct gaccgaaggc gcggcaaaag cgtggctgat caaacgcacc
     7741 gaaccgctgg cgcgttaaac agcacggcgc aaaaaatgcc gtctgaaagc ccttcagacg
     7801 gcattggttt cccaaaacat cccgcaccgt tttcatcttg ccgcaagcaa atatagtgga
     7861 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acgaatagta cggaaccgat
     7921 tcactcggtg cttcagcacc tgagagaatc gttctctttg agctaaggcg cgacaacgcc
     7981 gtactggttt ttgttaatcc gctatattcc g
//
LOCUS       DAWFIT010000093         7936 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFIT010000093 DAWFIT010000000
VERSION     DAWFIT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.093"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGQ85_001829"
     CDS             complement(27..443)
                     /gene="dksA"
                     /locus_tag="WGQ85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0751196.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(590..1381)
                     /locus_tag="WGQ85_001830"
     CDS             complement(590..1381)
                     /locus_tag="WGQ85_001830"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0751197.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1430..1837)
                     /locus_tag="WGQ85_001831"
     CDS             complement(1430..1837)
                     /locus_tag="WGQ85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0751198.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1837..2523)
                     /locus_tag="WGQ85_001832"
     CDS             complement(1837..2523)
                     /locus_tag="WGQ85_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0751199.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2643..3686
                     /locus_tag="WGQ85_001833"
     CDS             2643..3686
                     /locus_tag="WGQ85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0751200.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3849..5075
                     /locus_tag="WGQ85_001834"
     CDS             3849..5075
                     /locus_tag="WGQ85_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0751201.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5361..7511
                     /gene="yccS"
                     /locus_tag="WGQ85_001835"
     CDS             5361..7511
                     /gene="yccS"
                     /locus_tag="WGQ85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0751202.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1944 a         2624 c         1732 g         1636 t
ORIGIN      
        1 ttcggacggc attttgtttg ccgccatcag tcggcaaact gttttttcat cctctctcgg
       61 cgttcttggg actcaacaga taaagtggct gtcgggcgtg ccagcagccg cttcagaccg
      121 ataggctctc ccgtatcggc acagaatcca taatcccctt catcaatatt gcggatggtc
      181 gcctgtattt tactgagaag ttttcgttcc cgatcgcggg tacggagttc caatgcgtac
      241 tcttcttcct gtgtggcacg gtcggcagga tcgggggctg attcgtgttc ttggagatgc
      301 cctgtcgtag cggaagcatt ttcgatgagt tcgtcttgca tttttaccag caattcgcgg
      361 aaaaaagcca aatgctcgtc attcatataa tcgtcttccg gcccgttcca attcaaaata
      421 tcttgttctg tcagctttgc cataatgtat atcctctcgg ttcaccgaaa aaaacaaagc
      481 accacaaagt ggctttattc ctttgatttt tataaggctt attgcctagg gcgtttttga
      541 aaacgcgcat cataacacgt tttggattgg tttttttatt ttctttacat tattgatatt
      601 gccgttccat ttcctgcgaa cggcgcacac aggcacaaac gccctcactt atggcttcgg
      661 cgacacgatg ccgcctgaag gcttccacgg cttcgtgggt tgtcccgcct tttgacgtta
      721 catttttttg aagcttctcg aaatcttcac ccgtctgctc ggcaagggca accgctcctt
      781 taaacgttgc cagactgagc gcgcgtgctt ctgccatatc aaacccttgt cggatggcgg
      841 cattttgcaa tgcgtccagc agataaaaca catatgccgg cccgctgccg ctgataccgg
      901 taatgctgtg catttgcgct tcatcttcca accaaacagt caaaccgact gatttcatga
      961 ttcgatcggc aatcctgcgg tctgtttccg atacttccgc ttcggcatac ataccggata
     1021 cgcccagccc gatttttccg ggtgtattcg gcataacccg gacaatgcgg cgtgttcccc
     1081 cgaggtaacg gctgagcgta tcgaccggca atccggctgc gacagaaagc accaatgcgc
     1141 cgttggtgcg gatatttttg cacgcggctt ccatatcctg cggcttgacg gcaaggatta
     1201 aaacatcgtc ggaatgaagc tccggcaagg tttcagaagt ttcgaccccc aactcttttt
     1261 gcaaacgttc gcgcttttcc gcaccccggt tggcaatatg gatgcggtaa ccgccttgtt
     1321 tgaccaatcc gcccgcaacg gcagccgcca tattgccgcc gccgagaaaa taaacattca
     1381 ttatttctac cctattcaaa attacctgac taaattcatt cccaattttt taccatcgga
     1441 cgcgctcgga cagaccgttg cgccgcaacc cctgcttttt caaacaatca aaaccttcct
     1501 gtttgaacgt ctcaatcaaa ggtttgccgt cacgcgtacg ccaatccaac ccgcaggctt
     1561 caaacttccc tattttcctg taaaagtaag attgttgtgc ggattcatat tcctttgccc
     1621 tgacggaacg attgccgtct gaaagcggta tctcgtagtc ctctaaagac ggcggactct
     1681 cgccgccaat atcccaaaaa tcgatggcgg ccggttttct cggcttacac cagccggaca
     1741 gggacgaaca ctcataccaa cccgccaacc ggcagcctgc aaccgtaaag gcaagcaacg
     1801 cagccgttgc cgtatatttt attgcccgaa tttccatcat ccccttttcc cgaaaatcgc
     1861 gctgccgata cggacgtgtg tcgcaccgca ctcaatggcg gcaggcatat cgtccgacat
     1921 ccccatagac agcacgtccg ccttaacgcc agccgtattg aggtcggcaa gcaacttttg
     1981 catggtctga aactgggttt tcaactccgt ttcactgctg ttggctttgg caacgcacat
     2041 cagtccgcgc acgacaaggt tgggcaactt cgccacttcg accgcaagcg cgaccgcttc
     2101 ttcgggagca acaccgtgct ttgccgcctc gcctgcaata ttcacctcga tacacacctg
     2161 caaaggcggc attgaagaag gacgctgccc actcagccgg acggcggtct ttagacggca
     2221 tacggtatgc acccaatgcg cgcgttcggc gacaaacttg gttttgttgg actgcacatc
     2281 gccgatgacg tgccacacga tgtcggtcaa atccgccaac tcttccgttt tgccgtacca
     2341 ctcctgaata tagttctcgc cgaaatcacg ctgtccggcg gcgtaaactt cgcggatgcc
     2401 gtctgaaggg aaagtcttac cgacggcaat caggctgacg gaatgcggct tccttcccgc
     2461 ctgcagaacc aattttccaa tacggtcgga cacctcccga taacgttctt gcaacaccgt
     2521 catagattat cccctaatta aaaatgatta aacagttgaa acccctccag tcagggcggt
     2581 acaatcaagg ttgttagaac cattccaacc aatcgaaaca ttatactaaa cagagccgca
     2641 ttatgcagat taccgactta ctcgccttcg gcgctaaaaa caaagcatcc gaccttcacc
     2701 tgagttcggg catatcccct atgattcggg ttcacggcga tatgcggcgc atcaaccttc
     2761 ccgaaatgag cgcggaagag gtcggtaata tggtaacttc ggtgatgaac gaccaccagc
     2821 ggaaaatcta ccagcaaaac ttggaagtcg acttctcgtt cgaactgccc aacgtcgccc
     2881 gattccgcgt caacgccttc aacaccggcc gcggtcccgc cgccgtattc cgcaccattc
     2941 ccagcaccgt cttatcgctg gaagaattga aagccccgag cattttccaa aaaatcgcag
     3001 aatcgccgcg cggcatggta ttggttaccg gccctaccgg ttcgggcaaa tcgaccacgc
     3061 ttgccgcgat gatcaactac atcaacgaaa cccagccggc acacatcctg accatcgaag
     3121 acccgatcga attcgtccac caaagcaaaa aatccctgat taaccagcgc gagctgcacc
     3181 agcacaccct cagcttcgcc aacgcgctgc gttccgcatt gcgcgaagac cccgacgtta
     3241 tccttgtcgg cgagatgcgc gacccagaaa ccatcggctt ggcgctgacc gccgccgaaa
     3301 ccggacactt ggttttcggc acgctgcaca cgaccggcgc agcaaaaacc gtcgaccgta
     3361 tcgtggacgt attcccggcg ggggaaaaag aaatggtgcg ctctatgctg tccgaatcgc
     3421 tgaccgccgt catctcccaa aacctgctga aaacgcacga cggcaacggc cgtgtcgcct
     3481 cgcacgaaat cctgattgcc aaccccgccg tccgcaacct catccgcgaa aacaaaatca
     3541 cgcagatcaa ctccgtcctg caaaccgggc aggcgagcgg tatgcagacg atggaccaat
     3601 cgctgcaatc gctggtgcgc caagggctga tcgcaccgga agtcgcacgc agacgcgcgc
     3661 aaaacagcga aagtatgagt ttctgacaca caaccgcctt ccggccatac cggcgggaaa
     3721 acaaggcgca aacacgcggg gcgggacgca gcatcccgcc cggctaccct tccgaacagg
     3781 aagcgtccgc cttcctgttg aaacctgccg ccgcaaactg caaggcttaa accgaaaaga
     3841 agctaacgat gaataccgat aacctgcacg acatcttgga cgaaatggtt caagtgtatt
     3901 ctcaaaaaaa acaaagccga tccgaaaccc cggccgaaat cggcgcacac ttccacccgc
     3961 tgctcgaccg cctgtgcgaa accgcagaag cacaaaacgc gtccgacatc cttatcagca
     4021 aaggattccc gccctcgttg aaaatcaaca gcgcattaac cccgcagccg caaaaggcgc
     4081 tgacgggcga ggaaaccgcc gccatcgccg catcgacgat gaacgccgaa caatcggaaa
     4141 tattccggcg cgacggcgaa atcaactact ccgtccagtc gcgcagcggc acgcgctacc
     4201 gcgccaacgc ctaccacagc caaggcagcg caggtttggt tttgcggcgc atcaaccacg
     4261 tcatcccgca aatgcaggaa ttgggcctgc ccgaaaaact caaagacctc gccgtcgcac
     4321 cgcgcgggct gctgattatc gtcgggccta ccggttcggg caaatccacc acgatggcga
     4381 ctatgctcga acaccgcaac aaaaccctgc ccagccatat cgttaccatc gaagacccga
     4441 ttgagtttat ctacaaaccg cgccgctgca tctttaccca gcgcgaaatc ggcgtcgaca
     4501 ccataaactg gcagacggcg gtacaaaacg ctatgcgcca atcccccgac gtggtctgca
     4561 tcggcgaagt ccgcagcagg gaaagtatgg aatacgcgat gcagctcgcc caaaccggcc
     4621 acctgtgcat ttttacgctc cacgccaaca ccgcgccgca gtcgctcgaa cgcatactca
     4681 acttctaccc caaagaacag cacaaccaaa tactgatcga catcgccctc aacctgaccg
     4741 gcatcatctg ccaacgcctc gccctcaaac aagacaaaac gggcaggacg gcggttgtcg
     4801 acttgctcat caacacgccc gccatccaag acttcatcct gaagggcgac ctgatgaaca
     4861 tcagtaaaat catggaaacc gccaaaaccg acggaatgca gacgatggat caaaaccttt
     4921 tcgaactgta ccgtcacggc atcatcagtt acgaagaagc cctgcgccag tccgtttccg
     4981 ccaacaacct gcgattgcac atccaactgc acaaagaagg caaaacgccc gaactccttt
     5041 acgacagggt caacggtctc aacctcattt cctgatccgc aaaacccaat gccgtctgaa
     5101 aaccgcatcc ccgttttcag acggcatgat tttatccgcc ccattcatgt gctacacttt
     5161 atagtggatt aaatttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     5221 cagcacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
     5281 tttcagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata ttcacttcat
     5341 aacaataaac cggtaaaacc atgaaaaccc cactcctcaa gcctctgctc attacctcgc
     5401 ttcccgtttt cgccagtgtt tttaccgccg cctccatcgt ctggcagcta ggcgaaccca
     5461 agctcgccat gcccttcgta ctcggcatca tcgccggcgg ccttgtcgat ttggacaacc
     5521 gcctgaccgg acggctgaaa aacatcatcg ccaccgtcgc cctgtttacc ctctcctcgc
     5581 tcacggcaca aagtaccctc ggcacagggc tgcccttcat cctcgccatg accctgatga
     5641 ccttcggctt taccatttta ggcgcggtcg ggctgaaata ccgcaccttc gccttcggcg
     5701 cactcgccgt cgccacttac accacactta cctacacccc cgaaacctac tggctgacca
     5761 accccttcat gattttatgc ggcaccgtac tgtacagcac cgccatcatc cttttccaaa
     5821 tcatcctgcc ccaccgcccc gttcaagaaa acgtcgccaa cgcctacgaa gcgctcggca
     5881 gctacctcga agctaaagcc gactttttcg atcccgacga agccgaatgg ataggcaacc
     5941 gccacatcga cctcgccatg agcaacaccg gcgtcatcac cgccttcaac caatgccgtt
     6001 ccgccctgtt ttaccgcctt cgcggcaaac accgccaccc gcgcaccgcc aaaatgctgc
     6061 gttactactt tgccgcccaa gacatacacg aacgcatcag ctccgcccac gtcgattatc
     6121 aggaaatgtc cgaaaaattc aaaaacaccg acatcatctt ccgcatccac cgcctgctcg
     6181 aaatgcaggg acaagcctgc cgcaacaccg cccaagccct gcgcgcaagc aaagactacg
     6241 tttacagcaa acgcctcggc cgcgccatcg aaggctgccg ccagtcgctg cgcctccttt
     6301 cagacagcaa cgacaatccc gacatccgcc acctgcgccg ccttctcgac aacctcggca
     6361 gcgtcgacca gcagttccgc caactccgac acagcgactc ccccgccgaa aacgaccgca
     6421 tgggcgacac ccgcatcgcc gccctcgaaa ccggcagcct caaaaacacc tggcaggcaa
     6481 tccgtccgca gctaaacctc gaatcaggcg tattccgcca tgccgtccgc ctgtccctcg
     6541 tcgttgccgc cgcctgcacc atcgtcgaag ccctcaacct caacctcggc tactggatac
     6601 tactgaccgc ccttttcgtc tgccaaccca actacaccgc caccaaaagc cgcgtccgcc
     6661 agcgcatcgc cggcaccgta ctcggcgtaa tcgtcggctc gctcgtcccc tacttcaccc
     6721 cgtctgtcga aaccaagctt tggatcgtca tcgtcagtac caccctcttt ttcatgaccc
     6781 gcacctacaa atacagtttc tccaccttct tcattaccat tcaagccctg accagcctct
     6841 ccctcgcagg tttggacgta tacgccgcca tgcccgtacg catcatcgac accattatcg
     6901 gcgcatccct tgcctgggcg gcggtcagct acctatggcc agactggaaa tacctcacgc
     6961 tcgaacgcac cgccgccctt gccgtatgca gcaacggtgc ctatctcgaa aaaatcaccg
     7021 aacgcctcaa aagcggcgaa accggcgacg acgtcgaata ccgcgccacc cgccgccgcg
     7081 cccacgaaca caccgccgcc ctcagcagca ccctttccga catgagcagc gaacccgcaa
     7141 aattcgccga cagcctgcaa cccggcttta ccctgctcaa aaccggctac gccctgaccg
     7201 gctacatctc cgccctcggc gcataccgca gcgaaatgca cgaagaatgc agccccgact
     7261 ttaccgcaca gttccacctc gccgccgaac acaccgccca catcttccaa cacctgcccg
     7321 aaaccgaacc cgacgacttt cagacagcac tggatacact gcgcggcgaa ctcgacaccc
     7381 tccgcaccca cagcagcgga acacaaagcc acatcctcct ccaacagctc caactcatcg
     7441 cccggcagct cgaaccctac taccgcgcct accgacaaat tccgcacagg cagccccaaa
     7501 acgcagcctg aaaaagtttc ggcattttgt aagagagggc agattgtcag acaggttgca
     7561 agatagtgga taaagctttg cgcagggtaa atgcgtagca actgaaccgt cattcccacg
     7621 aacctacatc ccgtcattcc cacgaaaaca gaaaaccaaa aacagaaacc taaaatcccg
     7681 tcattcccac gaaagtggga atccagttcg ttcggtttcg cttgttttaa gtttcgggta
     7741 acttccactt cgtcattccc gcgcaggcgg gaatccagtg cgttgagctt cagctattta
     7801 gaataaattt tgaaactcta atcccgtcat tcccacgaac ctgcatcccg tcattcccac
     7861 gaaagtggga atccagaatc tcggactttc agataacctt tgaatattac tgttgttcta
     7921 aggtctagat tcccgc
//
LOCUS       DAWFIT010000094         7890 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFIT010000094 DAWFIT010000000
VERSION     DAWFIT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.094"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            296..1609
                     /locus_tag="WGQ85_001836"
     CDS             296..1609
                     /locus_tag="WGQ85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0751203.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1828..2003
                     /locus_tag="WGQ85_001837"
                     /pseudo
     CDS             1828..2003
                     /locus_tag="WGQ85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1999..3249
                     /locus_tag="WGQ85_001838"
     CDS             1999..3249
                     /locus_tag="WGQ85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0751204.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WGQ85_001839"
     CDS             complement(3284..4777)
                     /gene="rng"
                     /locus_tag="WGQ85_001839"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0751205.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4991..5248
                     /gene="grxC"
                     /locus_tag="WGQ85_001840"
     CDS             4991..5248
                     /gene="grxC"
                     /locus_tag="WGQ85_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0751206.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5269..5712
                     /gene="secB"
                     /locus_tag="WGQ85_001841"
     CDS             5269..5712
                     /gene="secB"
                     /locus_tag="WGQ85_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0751207.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5798..7840
                     /gene="recG"
                     /locus_tag="WGQ85_001842"
     CDS             5798..7840
                     /gene="recG"
                     /locus_tag="WGQ85_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0751208.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGTQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1649 a         2064 c         2337 g         1840 t
ORIGIN      
        1 tcccgcgtgt acggcacgcc ccgcgctccg caatgatagg ggcggtttcg tttgaatcgg
       61 cgcaccggga tgaatgcccc cgcgccgtcc cgataccgat ttgccggttg ttccgtatcg
      121 gttgccgccg tgaaatgacg gacggatgca ggaatggcgg caggcgtgaa tgggaggaaa
      181 tgaccgattg acttgtttca aaataaaaaa gtagaatgca gggcttatcc cgcgtctgtg
      241 ggcggttttg cctgcacggt cggttttctg tttaaaataa aataaaggag aaatgatgct
      301 gacgtttatc ggactgctga ttatcggggt catcgtatgg ttgctgctga cggaaaaagt
      361 gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg gcgttgctgg cggggtttga
      421 tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa tcggtgatgc agattgtgat
      481 tatgtttatg ttttccattt tgttttttgg aatcatgaac gatgtggggc tgtttcgtcc
      541 gatgataggc ggtttgatta agctgactcg gggtaatatc gtggcagtga gtgtggggac
      601 ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt gcgacgacgt ttttattggt
      661 cgtccccgcc cttttgccgc tttacaagcg tctgcatatg aatccttacc tgctgttttt
      721 gctgctaact tccagtgcgg gattgattaa ccttctgccg tggggcgggc cgactgggcg
      781 ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat aaacctttgt tgacggtgca
      841 aattatcggt gtagtgttta tccttgcgct gtccctgctt ttgggtgtgc gtgaaaaaag
      901 gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg gatttgataa agccggtgcc
      961 tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg ttttggtgga atgtcctgct
     1021 gtttttggcg gcgatgagcc tgcttttttc gggcatcttc ccgccgggtt atgtatttat
     1081 gctggctgca acggcggcgt tgcttttgaa ttaccgcagc ccgcaggaac agatggagcg
     1141 gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc attattttgg cggcaggtac
     1201 gtttttgggg attttgaagg gtgcggggat gttggacgcg atttccaaag acattgtgca
     1261 tatcctgccg gacgcgctgc tgccttatct gcatattgcc atcggtgtgt tgggcattcc
     1321 gcttgagttg gttttgagta cggacgctta ttatttcgga ctgtttccga ttgtggagca
     1381 gattacctcg caggcgggcg tggcgcccga agcagcaggt tatgcgatgt tgatcggcag
     1441 tatcgtcggc acttttgtta cgccgctttc gccggccttg tggatgggct tgggtttggc
     1501 gaaattgtcg atgggcaaac acatccgtta ttcgtttttc tgggcgtggg gtttgtcgct
     1561 ggcgatattg gccagttcga tagcggcagg aatcgtgcct ctgccgtaaa cggcgaagcc
     1621 gtctgaagcc tgaatcgttc ctgtggtctg tcgcaggctc tgcctgcccg tgtgttgcaa
     1681 tcgcgcccca atgccgtctg aaggcagttg ccggcgcggt ttttttgtgc cgcagttttg
     1741 aaaaataccg gcaagcgtaa aggccgctaa attgcggcta attttggggt gttagaatag
     1801 gcacggttta ttttgaaggg aaagttgatg cgtgttttgc tggtggaaga cgatgcgatg
     1861 attgcggagg cggtgtcggc aagtttgaaa gacggcggct atgcggtgga ttgggtcaaa
     1921 aacggcgcgc ggctgccgct gcccagcctt atgacttgat gctgctggat ttgggtttgc
     1981 cggggcggga cggtttggat gtttgttccg ctggcaatgc ttgcaggtat gttttcctac
     2041 tacgaaacct tccacgaaac ggaagcgttg caggacgacc tgctccgtca ggcggcattg
     2101 tacgtcgcac ccgattctaa acccgaaact ttgcccgagg gcgacggcga tacgcgcatt
     2161 ttcgtgcaga tgccgcagca ggaagaccct gttgtcagcc tgcctgcgca tctggcggac
     2221 ggtctgcaca cgcttcaggc ggacggggac gacgattatt accgcgtcta tatccgcacg
     2281 accgaacagg gacggattgc cgttatgcag gaaaacgaat accgtgaaga tttggcggcg
     2341 gatgcggcac ggcaaagcgt gttgcccctg ctggcggcac tgccgctgat gatactgctg
     2401 accgtgtgga ttacgcacaa agccatgcgc cccgtccgca aattgtcgca aagtctcgaa
     2461 caacgccgaa tcaatgacct gtctgctttg agtgtggaca atattcccag tgaaatcaga
     2521 gggttcgtaa ccgccatcaa cctgcttttg aaacgtgcgg atgaagacat ccgccaccgt
     2581 cagcggtttg tcgccgacgc ggcacacgaa ttgcgtacgc cgatgacagc cctttccctt
     2641 caggcggaac ggctcaacaa tatgtcgctc ccacccgatg cagcgcggca gtctgccgtt
     2701 ttgcagcaga gcatcaggcg caacaaacac ctgctcgaac agcttttggc actggcgcgt
     2761 tcgcagtcgg atgaaacccc tctgacgaaa acgacattcg gtctgcaaag ccgtttccgc
     2821 caagtgttgc aggaactgat gccgctggct ttggaaaaac gtcaggacat cggcgtggcg
     2881 gtcggaggcg atgtcgaagt gtctgccgac gaaacggaaa tctatacgct gattaaaacc
     2941 tttgccgaca acgcggtacg ttacacgccg cccgaaggca ggatagattt gggtttcacg
     3001 gacgaaggga aatatctcgc cgtgtgggtg gaagataacg ggaacggcat tcccgaatcc
     3061 gaacgcgccc gcgtcctcga tccgttttac cgtattttgg gaacggagca gcaggggacg
     3121 gggctggggc tgtcgattgc cgacacgctt gccaaaaaat acggcggaca tttggaattg
     3181 accgacagcc gacgtttcgg acacggtctg ttgatacggg cgttgttgga caaggaaacc
     3241 ctgaaataga cagatgccgt ctgaaggctt cagacggcat tttttacatc agcacaatat
     3301 cgtattgttc ctgcgtgtaa gcggtttcga ccgccagaga aatcggtttg ccgatgaaat
     3361 ctatcagcat tgccaaggat tgcgattctt cgtccaaaaa caaatcgatg acgttggggg
     3421 cggcgaggat gcggaaactt tcggcatcgt aacggcgcgc ttcgcggacg atttcgcgct
     3481 ggatttcgta gcacacggtt tgcggcgttt tcagacggcc tctgccttgg caggaagggc
     3541 agggttcgca gaggacttgg tttaagtttt cgcgcgagcg tttgcgcgtc agctcgacca
     3601 gccccaggct ggtaaaaccg tgcagggtaa cgcgggtacg gtcgaaggcg agggctttgg
     3661 caagctcctg caacacggct tcgcggtggc tttcctgcgc catatcgatg aagtcgatga
     3721 tgatgatgcc gccgaggttg cgtagcctca attcgcgggc gatggtgtgg caggcttcga
     3781 ggttggtgcg gaagatggtt tcgtcgaagt tgcgtgcgcc gacgaagccg ccggtgttca
     3841 cgtctatcgt ggtcatggct tcggtggatt cgataatcag gtagctgccg aagttgaggt
     3901 tgacgcgcgg ttgcagggcg cggctgattt cctgttcgac gttgtgggtt tcaaacagcg
     3961 ggcgttcgcc tttgaacaat tctatcctgc ccaatgcgcc gtggacgtat tgttcggcaa
     4021 aacgcgtcat gcgcccgtgg tttacggtgg aatcgacgag gattttttgc gtgtcgcagc
     4081 cgaccatatc gcgcaatacg cgcaaactca agggcaaatc ctgataaagc agggtttccg
     4141 gcggccggat tttcgcctgt tcttggatgt gttcccacac tttggtcagg tagtcgatgt
     4201 cggactggag ctgttcgtcg gtggcgtttt cggcgttggt gcggatgatg tagccccggc
     4261 aggcattttc cggcaggagc ttgtcgaggc gttcgcgcag gctgctgcgt tcggcatcgt
     4321 cttcgatgcg ttgggacacg ccgatgtggt cttcttgcgg aagatggacg aggaaacgcc
     4381 ccgccagcga gatttgggtg gaaagccgcg cgcctttggt gttgatcggg tctttgatga
     4441 cctgcaccaa aacagactgc ccttcaaaca gcatatgttc gatgcgctgg gtttcttcgg
     4501 ggttgcggcg ttgttcgagg acatcgacga tgtgtaaaaa cgccgcgcgt tccaagccga
     4561 tgtcgataaa cgcgctctgc atcccgggca gcacgcggcg caccacgccc agatagatat
     4621 tgccgaccag gctgtgttcg ctgttgcgct cgatgtgcag ctcgcagata ttgttttcct
     4681 ccaacaccgc tacgcgcgtt tcctgcggcg tgatgttgac caatatcgtt tcgggcgggc
     4741 gcgcgatgtc tttggggatg gggagtcctg acaacatggt ttttcctgaa aatgtaataa
     4801 aaatattttt gcacccgttc cgccgcgcag cgcgggtgca aaaatatttt tcacaatgcc
     4861 tatcatactt taaaaaagaa actttgacac attccgcaca tgccgtttga acgtgcatct
     4921 gttccgcact tgccaaacag gggaaatgcc cctatattga ttatcattgc aaaactttcg
     4981 gaaacccaat atgcagaccg ttaccatgta cacaggtccg ttttgtccct actgcgcgat
     5041 ggcgaaaagg ctgctgcacg cggcaggtgt cggacatatc gacgaaatcc gtgtcgatgc
     5101 aagccccgaa gcctttgccg aaatgcagca gctttcggga cagcgcagcg tgccgcagat
     5161 tttcatcggc gaaacgcacg tcggcggatt taccgacctc taccgcctcc agcaggaagg
     5221 cgggctggac ggactgctga acccttaacc cccaactagg aaaacaaaat gagcgaagaa
     5281 ctgcaacccg tattcagcat cgagcgactg tatgtcaaag acttgtcttt ggaagtgccg
     5341 cacgcgccgc aaatcttttt ggaacagggc gagcctgaag tggatatgcg cgtttccacc
     5401 ggcagccaaa agctggaaga cggctactac aacgtggacg ttaccgtaac cgttaccgcc
     5461 aagctggata acgagcgcac gatgtttttg aacgaagtaa cccaaagcgg catcttccgt
     5521 ttggaaaaca tccccgaaga agatgtgcag ctgctgctgg gcgtggcgtg tccgaacatc
     5581 ctcttccctt acgcgcgcga agccgtttcc ggtacggtaa cacgtgccgg cttcccgccc
     5641 gtcctgctcg cgccgattaa ttttgaggcg atttaccaac aacagcagga agccgaagcc
     5701 gccggggctt gattcctgcc tgccgatgcc gtctgaatcc gtttcagacg gtatttttgt
     5761 tttcggataa aataggtgcg ttcaaaggaa tctgcctatg atgtcgcccg aaacccagaa
     5821 acagctcaaa atcaccgatg tttccgccaa gaagctcgac aaactcaacc tccataccgc
     5881 gtgggatttg gtgttgcacc tgccgctgcg ttacgaggac gagacgcaca ttatgccgat
     5941 taaggacgcg ccgattggcg tgccgtgcca ggtcgagggc gaggttatcc atcaggaagt
     6001 aacgttcaaa ccgcgcaagc agttgattgt ccaaatcgcc gacggttccg gcagcgtcct
     6061 ttttctgcgc ttcatccact tttacgccag ccatcagaaa cagacggcgg tcggcaaacg
     6121 catccgcgcc gtgggcgaaa tcaaacacgg cttttacggc gacgagatga ttcatcccaa
     6181 aatccgcgat gccgagggcg gcggtttggc ggaaagcctc acgccggttt acccgaccgt
     6241 aaacggtttg aaccagccca ctttgcgccg tattattcag acggcgttgg acgttacgcc
     6301 gctgcacgac acgctgcccg atgccctgct gtgccgtctg aagctgccac acctcgccga
     6361 aagcctgcgt cttttgcatt cgccgccgcc gagtttcacg attcatcagc tttcagacgg
     6421 cacgctgcct gcatggcaac ggctcaaatt cgatgaactt ttggcgcaac agctttcgat
     6481 gcgcttggcg cgacagaagc gtatcggcgg cacggcggcg gcattgggcg gcgacggcac
     6541 attgacccaa gccctgcgcc aagccctgcc gtttgccctg accgatgcgc aagaaaaagt
     6601 tgtttccgaa atctgccgcg atatggcgca aacctacccg atgcaccgcc tgctgcaagg
     6661 cgatgtcggc agcggcaaaa ccattgtcgc cgccttgtcc gcgctgaccg ccatcgaatc
     6721 cggcacgcag gtggctgtaa tggcgcccac tgaaatcctt gccgaacaac attttattaa
     6781 atttaaacaa tggctcgaac ctttgggtat tgaagttgtc cgcctttttg gcagtttgcg
     6841 taaaaaagcc aaagacgaag ccaaagccaa actcgccgac ggcagcgtca aaatcgccat
     6901 cggcacgcac gccctgtttt cagacggcgt ggcgtttcac aatttgggct tgaccattgt
     6961 ggacgaacag caccgtttcg gcgttgccca acgcctcgcc ctcaaaaaca aagggcgtga
     7021 agtccatcag ctgatgatgt ccgccacgcc catcccgcgc acgcttgcga tgagtttttt
     7081 cgccgatttg gacgtgtccg tcatcgacga attgccgccc gggcgcaccc cgattaaaac
     7141 gcgcctcgtc aacaacgtcc gccgcgccga agtcgaaggc ttcgtcctcg gcacttgccg
     7201 aaaagggcgg caggcgtatt gggtctgccc attgattgaa gaaagcgaaa ccctgcaact
     7261 gcaaaccgcc gccgaaaccc tcgcccggct tcagacggca ttgcccgaac tcaatatcgg
     7321 actggtacac gggcgcatga aggccgccga aaaggccgaa gtcatggcgg aatttgccgc
     7381 aggccgtctg aacgttttgg ttgccaccac cgtcatcgaa gtcggcgtag atgtgcccaa
     7441 tgccgccctg atggtcatcg aacacgccga gcgcatgggc ttggcgcagc tgcaccaatt
     7501 acgcggtcgg gtcgggcgcg gcgcggcaga aagcgtgtgc gtcctcctgt ttgccgaacc
     7561 cttgggcgaa ctcgccaaag cgcggctgaa agtcatctac gaacacaccg acggctttga
     7621 aatcgcccgc caagacctca atatccgcgg cccgggcgag tttctcggcg cgcgccaaag
     7681 cggcgtgcct atgctgcgtt tcgccaaact cgaagaagac ctgcaccttt tggaacaggc
     7741 gcgcgaaacc gccccgatgc tgattgagca aaaccccgaa atcgtcgaag cgcatttggc
     7801 aaggtggctt tccggcaggg aagggtattt gggcgtgtga gcaaaatgcc gtctgaaata
     7861 ttagaaaaaa aagtgtaacg gaaatctgat
//
LOCUS       DAWFIT010000095         7618 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFIT010000095 DAWFIT010000000
VERSION     DAWFIT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.095"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WGQ85_001843"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WGQ85_001843"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0751209.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1852..2133)
                     /locus_tag="WGQ85_001844"
     CDS             complement(1852..2133)
                     /locus_tag="WGQ85_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0751210.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="WGQ85_001845"
     CDS             complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="WGQ85_001845"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0751211.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3495..4433)
                     /locus_tag="WGQ85_001846"
     CDS             complement(3495..4433)
                     /locus_tag="WGQ85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751212.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4740..5852
                     /gene="nadA"
                     /locus_tag="WGQ85_001847"
     CDS             4740..5852
                     /gene="nadA"
                     /locus_tag="WGQ85_001847"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0751213.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKTEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            5904..7412
                     /gene="nadB"
                     /locus_tag="WGQ85_001848"
     CDS             5904..7412
                     /gene="nadB"
                     /locus_tag="WGQ85_001848"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0751214.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLNRQTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPTLQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYARRQNIGAHFNSDC"
BASE COUNT         1767 a         2083 c         2045 g         1723 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagtctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc gacacggaca tccttcaacg cctccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcacc cagcagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaactccg tcagtgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac actataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcttatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgcccccg tcctttcccc
     2341 tgccctaccg atgcctccgc gctgtcgggc aaacaaaata cctgcctgaa acgctgtttt
     2401 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattt
     2461 cctccttgag gcaataaccg gattacggtt aggcggttcc ttgtctgaca tatcagacaa
     2521 ctgccgacac cctaaaactc atgccaagaa atccaaacct atgtccaacg agcggctgct
     2581 gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg
     2641 gtcgaagccg atgccgcccg atgcttcgca atagacggtg tgggggtggg cggtttgcgt
     2701 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg
     2761 ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaac gtgtccactt cgatttccac
     2821 gcaggtcaat gctccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca
     2881 ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aagcccatgc ggtggttcac
     2941 gccgccgcct gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg
     3001 gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcacgcg cggtggcggt
     3061 ggcgatgccg cttaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc
     3121 gcgggcgttg ccttcgacgg cagccagcgt ctgacctgcg cggacgactt gcccgtctcg
     3181 gatttcggct tggaagcgaa cggacggatc catcgtctga aaggcgaggc gcgccaagtc
     3241 catgccggcg ataacgccgt cttcgcggct gacgaggaag agtttggcgg ttttgtcggg
     3301 ggcgataacg gcggcggacg taatatcgcc gcgcctgccc aaatcttcgc tcaaggcttg
     3361 ttccaccatg gggcgcaaca gggtgtcggg cagggggaag agggtgtttt cagacggcat
     3421 ggggtttcct ttgttgaaaa agattgatgt tgcaccgatg ggcaaaactt cgtttgatgt
     3481 cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat cagcctgtcg
     3541 ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct gcccgataca
     3601 ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa gttttgcttg
     3661 tgcagcaatc tgccgctgat ggcttcgaca ctgttttgca gttgcagcag cgtgaattcg
     3721 ggcggcatca gttcaaaaat cacggggcgg tatttgattt tggcgcgcag gcgagacagg
     3781 gcggtcgcca gtacgcggcg gtggtcgtgg cgcatgggct gccccgtaag cgcgaagtcg
     3841 aagtttgcct gcggctcggc ggcctccgct atcaggccgc tttcatacag catttcatag
     3901 cgttgcaaaa cgtattcttc cgaccagttt tccggttcga ccccccaaca caaatgaatg
     3961 cgcttgagcc gcttttggcg cacttcctcc gtgtccgccg agtttgccca aatgcgcagg
     4021 cggccgacga cggcgtcgcg ctgcccgccg tcggtacgca aatcttccca cgggaaatag
     4081 ccgtagcagt cctgccattt cgcgtcggga tgcaggatgc tgtcggctgc ctcgcgcacc
     4141 agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg gtgggtatcg
     4201 acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt ggcgacccac
     4261 agtttcacgc ccgcctgcag ggaattgcgc aggggggaga gcgggccgtt gggcagaagc
     4321 gtgccttggg cgacggtcaa tacccgcagg ccgccgtcgg taacggcaat caatacggga
     4381 accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc catgctcggc
     4441 tttctgcggg tgggaaaggc tttattgtat agtggattaa atttaaacca gtacagcgtt
     4501 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     4561 ttaatccact ataaaccaaa gcggtttgtc ggaacgaagc gggtcggttt tacggtatcg
     4621 caaacctgtt ttcagacgac cttgcttatt atactcaatt tgagcataat ataaaaatat
     4681 ttcaaaatat aaacggcaaa tctttatgcc gtctgaaaaa ccagccataa ggaagtctca
     4741 tgcaaaccgc cgcccgccgc tcgttcgatt acgatatgcc cctcatccaa accccgactt
     4801 ccgcctgcca aatccgtcag gcgtgggcga aggttgccga cacgcccgac cgcgagacgg
     4861 caggccgtct gaaagacgaa atcaaggctt tgctgaagga gaaaaacgcg gtcttggtgg
     4921 cgcattatta cgtcgatccg ctgattcagg atttggcttt agaaacgggc ggatgcgtgg
     4981 gcgattcgct ggaaatggcg cgcttcggcg cggaacacga agctagtaca ttggtggtgg
     5041 cgggtgtgcg cttcatgggc gagagcgcga aaatcctctg ccctgaaaaa acagtgctga
     5101 tgcctgattt ggaggcggaa tgttctttgg atttgggctg tccggaagaa gcgttttcag
     5161 cgttttgcga ccaacacccc gaccgcacgg tcgtagtgta cgccaacact tccgccgccg
     5221 tgaaagcgcg tgccgactgg gtggtaacgt cttcggtggc gttggaaatc gtatcgtatt
     5281 tgaaatcgcg cggcgagaag ctgatttggg gacccgaccg ccacctcggc gactacatcc
     5341 gccgcgaaac gggcgcggat atgctgttgt ggcagggttc gtgcatcgtc cacaacgaat
     5401 tcaaagggca ggagctggcg gcgttgaaga cggaacaccc cgacgcggtg gtgctggttc
     5461 atcccgaatc gccgcaaagc gtcatcgagc tgggcgacgt ggtcggttcg accagcaaac
     5521 tactgaaagc cgccgtatcg cgtcccgaaa agaaatttat agtggcaacc gatctgggca
     5581 tcctgcacga aatgcaaaag caggcgcccg acaaagaatt catcgccgcg ccgacggcag
     5641 gcaacggcgg aagctgcaaa agctgcgcgt tctgcccgtg gatggcgatg aattcgctgg
     5701 gcggcatcaa atacgccctg acaagcggac gcaacgaaat cctgttggac agaaagctgg
     5761 gcgaagccgc caaactgcct ttgcagcgta tgctcgactt cgcggcaggg ctgaagaaaa
     5821 aagatgtgtt caacggcatg ggccccgcct gatttgccgt ccatattgcc gtttcagaca
     5881 acctctcaaa caaggacaac accatgcaaa cggattgcga cgtattgatt gccggaaacg
     5941 ggctggcggc actgacgctc gccctgtcgc tgcctgaatc gttccgcatc gtcattttgt
     6001 gtaaaaaccg gctggacgac accgccagcc gtcatgcgca aggcgggatt gcggcggcgt
     6061 ggtcgggaga ggacgacatc gaaaaacacg ttgccgatac cttggaagcg ggcgcgggtt
     6121 tgtgcgacga agccgccgtc cgcaccatcc tgtcgcaggg caaaccggca atcgaatggc
     6181 tgctggcgca gggcgtggcg ttcgaccaaa ataataacgg cctgcacctg acacgcgaag
     6241 gcgggcatac ctgccgccga atcgcccacg tcgccgatta cacgggcgaa gccgtcatgc
     6301 agagcctgat tgcccaaata cgccgccgcc cgaacatccg cgtttgcgag cggcagatgg
     6361 cgttggacat ccaaaccgaa tcaggcgcgg cgtgcgggct gaccgtcctc aaccgccaaa
     6421 cgcaagaaac ctaccgcatc cgcgcccgcc ataccgtact cgcaggcggt ggtttgggac
     6481 agatttacgc cgccaccacc acgccgcccg aatgcacggg cgacgccatc gccatggcaa
     6541 tacgcgcagg ctgcgcagtc gaaaacctcg aatttatcca attccacccc acaggcttgg
     6601 caaggccgtc tgaaaacggc cgcaccttcc tgatttccga agccgtgcgc ggcgaaggcg
     6661 gcatcctgac caaccaagcg ggcgaacggt ttatgccgca ttacgaccgc cgcgccgaac
     6721 tcgcaccgcg cgacatcgtt gcccgcgcca ttgccgccga aaccgccaaa caaacgcaag
     6781 acttcgtctc gctcgacatc agccatcaac ccgcagcgtt cgtccgccag cattttccgt
     6841 ccatccatcg acactgttta tcccaatgcg gcctggatat cacgcgccaa gccatccccg
     6901 tccgccccgt gcaacactac acctgcggcg gcatccaaac cgaccccagc ggcagaactt
     6961 ccctgccgca gctctacgcc ttaggcgaaa ccgcctgcac agggttgcac ggagccaacc
     7021 gcctcgccag caactcccta ctcgaatgcg tcgttaccgc caggcttgcc gcccaaacca
     7081 tcgcggatgg acaagcgttc caaaccgtac cgtccaaaag gtcgtctgaa aacacctccg
     7141 ccgaagcaga catcttttca gacggcatcc aaaacacatt cagccgcccc accctgcaag
     7201 cgttcaacca acgccatctg ggcatcctcc gcaacgatac cggcctgcgc cgcgccatcg
     7261 cccaactgcg gctttggaag caaaaccaaa ccgagccgca cactgcgtcc gaatacgaaa
     7321 accgcaacct gctcgaatgc agcctcgccg tcgcccaagc cgcatacgca aggcggcaaa
     7381 acatcggcgc gcattttaat agtgattgtt aaggttggca ggtcagagaa aatcgttcaa
     7441 ataaatttga atagcatggt taagaaattg gcattcggtt tttagggttg cgtaagttgg
     7501 gttatcaatc tgttctaaaa gcagaaaggc cgagaccttt gcaaaattcc ccaaaatccc
     7561 ctaaattccc accaagccat ttaggggatt ttccatgagc accttcttcc ggcaaacc
//
LOCUS       DAWFIT010000096         7478 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFIT010000096 DAWFIT010000000
VERSION     DAWFIT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.096"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..434
                     /locus_tag="WGQ85_001849"
     CDS             <1..434
                     /locus_tag="WGQ85_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751215.1"
                     /translation="KNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGF
                     NGDIGEKLHRCAVMEWAENETETFAKFLSLPTAETQTQVFSCFRPKYLLILPKYPLNP
                     ARIPDNQASGLLFRRQRAHLACWPLSTGSNTSPSGGFAHSL"
     gene            complement(346..1353)
                     /locus_tag="WGQ85_001850"
     CDS             complement(346..1353)
                     /locus_tag="WGQ85_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0751216.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGLPKGTTVYADKGYDSAENRQHLEERQLLDGIMRKACRNRPLTEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAA
                     A"
     gene            complement(1623..3710)
                     /locus_tag="WGQ85_001851"
     CDS             complement(1623..3710)
                     /locus_tag="WGQ85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0751217.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(3713..4978)
                     /locus_tag="WGQ85_001852"
     CDS             complement(3713..4978)
                     /locus_tag="WGQ85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0751218.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLQTMGVVRVYSPDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRPRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(4945..5159)
                     /locus_tag="WGQ85_001853"
                     /pseudo
     CDS             complement(4945..5159)
                     /locus_tag="WGQ85_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6201..6437)
                     /locus_tag="WGQ85_001854"
     CDS             complement(6201..6437)
                     /locus_tag="WGQ85_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0751219.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
BASE COUNT         2247 a         1392 c         1431 g         2408 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttcgcccca aatatctcct aattctaccc aaataccccc
      301 ttaatcctgc ccggatacct gataatcagg catccgggct gctttttagg cggcagcggg
      361 cgcacttagc ctgttggccg ctttcaacag gttcaaacac atcgccttca ggtggctttg
      421 cgcactcact ttaatcagtc cgaaataggc tgcccgcgca tagcggaatt tacggtgcag
      481 cgtaccgaag ctctgttcga ccacataacg ggtcttcgac aaataccggt tgcgtttggt
      541 ttgcgcttcc gtcagcggac ggttgcggca ggctttgcgc ataatgccgt ccaacaactg
      601 acgctcttcc agatgttgcc ggttttccgc actgtcgtag cctttatcgg catagacggt
      661 cgtacctttg ggcagacctt ccaacaacgg cgacaggtgt ttgcactcat gggcattggc
      721 gggggtaatg tgcagtttct cgatatagcc ttccgcatcg gtacgggtat gttgtttgta
      781 accgagtttg tagaggccgt ttttctttat ccaacgggca tctttgtcct tactcggtgt
      841 ggtttggccg ctgatttgtc cttcttcatc gacttctatg gcctgacgct gtttgctgcc
      901 ggcggtctga ataatggtgg cgtcaacgac ggcggcggat gctttctcta cttttaggcc
      961 tttttcggtc agttgtcggt tgatcagttt gagcaattcg gacagggtgt cgtcttgcgc
     1021 cagccagttg cggtagcggc ataaggtgct gtaatcgggg atgctcagtt cgtcaaaacg
     1081 acaaaacagg ttgaagtcga tgcgggtgat gaggctgtgt tcgagttcgg gatcggagag
     1141 gctgtgccat tgtccgagca ggacggcttt gaacatggac aacaggggat aggcgggacg
     1201 gccgcggtgg tctctgaggt aacgggttct ttgacggttc aggtattgtt cgatcggttg
     1261 ccaatcaatc acctggtcca acttcaatag tgggaaacgg tcgatgtgtt tggcaatcat
     1321 ggcttgggcg gtttgttgga agaaggtgct catgagaaat cccctaaatg tcatggtggg
     1381 aatttaggga attttgggga attttgcaaa ggtctcagac cgtgaaatta gcccaaaaat
     1441 gggaacaaga gcagaaaaaa caacaaattc aacagaaaaa ggaaactgaa aaatcgccaa
     1501 aacacaaagc cagtcgtgat gattgggaaa tggaacgtta aaccgctatt ttcatgtatt
     1561 ctattaaatt atttgaaaaa agacttcact ttttgttaaa gaaaaatgaa gtcttgttct
     1621 gttcaaccta aattcagaat tttatctgcc gattgaattg tttcttggcg gtgtgcaacc
     1681 attattttca taataccaag actttttagg ttatggttaa ttttttgttc attttctaca
     1741 tctaaatggc tacttgcttc gtctaaaaat agaattttgg gtcgtttata caatgcacga
     1801 gccaagataa ctctctgctt ttgtccacct gataagatat ttcccatatc gccaatcaag
     1861 gtctcatagc ccattggcat tttaagtata tcgtcatgta tttgtgccat ttttgcacat
     1921 tgttcaatga gctccatatt tgggctttca tcaaaaaatg aaatattttc cccaatagaa
     1981 cctgcaaaaa ggacatcatc ttggcgaaca atcccgctca atcccctaat aaaggatgga
     2041 gaaacttgat atatatcatg cccattaata ctaactgtac cagtttcagg ttttaggcta
     2101 cctgttaaaa tgtttaacaa agtggacttc ccccgaccag attgtcctgt taaaacaact
     2161 gcttcattat ctttaaattc caaattaatg ttttcaaaaa gatatggctc attatcagca
     2221 tatctgaatg agacgttttc aactttaaga accagttgtt cattatctaa tttaggtata
     2281 tgattatact taataatttc agtttctgtt tcatttaaag taatgtcagc caaacgttca
     2341 gcatgaagcc ctaacatttt gatttggatg tattggtcaa cgagagaagc tgttctgctt
     2401 tcaaattgcc ctttataagc caaaaaagcc atcagaacac cgactgtaaa tgaaccatct
     2461 aaaattgcgc ttgcaccaag ataaattatg ataatatttt ccatgctaaa caacagttta
     2521 tttgaaaatt caaataaagc agagagttta tctgttgtca gcttggtatt gactgtattc
     2581 acaaataggc tcatccaagt gccatgtctt tgataatgtt tatcaaataa tttaactgat
     2641 tggataccac gaatggtttc catgaaatat gagttttgtt tggcttcatg aacaatattt
     2701 tcttctgttg catttcttaa tgggtaatat gcaagccaac gaattagtat gtacaaaaca
     2761 agtgttaaaa gaacaatcag cgataattga gtgctgtaaa ttgtcattaa cacgaaagta
     2821 aaaacagcca ttaagctatt taaaactaaa acaaaaaaag tagaagttag tgtttcttgg
     2881 atatgatcta ttgaaccaaa tcttgaaatc acatctccta aatgtcgttt actgaaatag
     2941 tcattaggta agtcaagtaa ccttttaaat atattggctt tccattgtaa attaagagtt
     3001 gtagataggt gcatacctac ccatgcttgt aacaggctaa ttaactgttg caggatagcc
     3061 agtaaaccaa atcccaaagt aagggtcagt aataaatttt tatcagcagt tacaatgaca
     3121 tggtctatta cccattgcat aaagaatgga ctaaccaatg caaagacttc caaagaaata
     3181 gctaatataa gcatttgaat taaagagcgt tttaagcctg aaatccccct taatagagat
     3241 aatattttga ttttctttgt ttcttttttc tcttcaaaat gggtattggg gaataattct
     3301 agggcaatcc ctgtgaattt ttgtgaaact tcgtccattt tgatttttcg cataccgaca
     3361 gcagggtcca taatgacgat actgtctttg gaaatggaac aaagtacaac aaaatggttt
     3421 aagttccaat ggagaatgca gggtagttgt aaatttgaca gctcatctaa ctctaaacgc
     3481 aaagctcgtg gcgttaaatt catttcattg ccaaatctca tgatgtctgc aagatttgcg
     3541 ccctttaatg acagggtgta tttttggcgt aacgttctta aatcagtgtg aaagccataa
     3601 taggacaaga tggatgtcag gcatgctaaa ccacattcag caacttctgt ttgcagaatg
     3661 acaggtagct ttttgttaaa tccaaaggac agtcttgata aataatccat ttttaattga
     3721 tttttcctga aatgctgtaa agtgggtcaa gtacccattc gtacaatttt tttcgttcat
     3781 ggagaatatc tgcttctaaa atcatgccaa tttgaagcgg cttgttttct ccgtatgctt
     3841 taatcgtttg tttttccaat ttaactttca caagataggc aggttcattt aataaggttg
     3901 ggttagtgaa aatgatacct aaacctgata gctcttgttt accgagagca gttctggcaa
     3961 ctgaaataat ttctcctgtg gcatgtccaa atttttggta agggtacgct tggtaacgta
     4021 aaacaacttt atctttcggt ttaataaaac caacagcttt actgggtatg taaagattgg
     4081 cgaccaattc agtttgttca gggacaatgc ttaacagcaa tttagacggt tcaacttgtt
     4141 gccctatatc aacattaatt gttgatatat aacctgattt actagctcgt atggtttgtt
     4201 cggatttcaa atcaaaatcc aaaatttctt ggttcatttc cgtaatcgca cggttgagtt
     4261 ggctcaattc ggttttatgg cgttcaggca ggctgcttaa tgtaattttc tgttcatcaa
     4321 gttccctgat tgcattattt tgttcaagtt ttaggctctc caaacgtggg cgttgttcca
     4381 ataaatggct ttcggcggtc atcttatctt gttgggatac tgcgccttga ctggctaaaa
     4441 acttgttctt gttaagggtt ttttccgcta aacgaatttg acgattttgc cctgtaattt
     4501 gctgtttaat attctctaat tgattgttta aacgatgaat gttgttatga acattttttt
     4561 gctcattttg atgaatgcgc tttaaacgtt ccaattcttg taatgccaaa gtttttttaa
     4621 ggttggcttc tgctgccaat ttggcttgta cgtttccttt ttcgccaaaa cgcgatgtgg
     4681 aaagtttgaa caatggttcg ccagctttga caaagttacc atcttcaaca aatttatgcg
     4741 taatcgtgcc gatatcggga gagtaaacac gaaccacccc catagtttga agtaattgac
     4801 cttcaacggt tgttttattg gtatagctac caaaaatcaa aaagataatg atacacagag
     4861 caatgagaaa agcgcaaaaa gtcagaaata aaaaagagaa tggacgggtc aagataacct
     4921 gacctgtcca cttattttgt tgggctacaa agacttcttt tcggaataag tgggacattt
     4981 attattattt cttaaaaaaa tggatagaaa aacaccaata attaaatact tatgcctaat
     5041 aacataaatt tgctattgat tgatgtttct attttaatta cattaaattt aaccaataat
     5101 ccagaaatgg ttgcagctaa aatgcaaaag acagtggcgt aaatggtttc agtattcata
     5161 atttttctct ttcaaaattg ttcaatctaa atagaaaaag gtaacttcaa tacatattgc
     5221 tgtaactgaa attacctttt ccttaatgat gtaattatct tatggcttca tcatgccacg
     5281 accgatattg tatccgccag cagcagagaa aataccagct ccaattgcgc caacccagcc
     5341 tactggtcct gtccacttgt tccgttgagc tacaaagact tctggtctga aaaatgagtg
     5401 attgttagac atgtgtactt atttatgttt ctttgattgg gataaatttg taaaaacaaa
     5461 aaaaaaggca agttgttatt atagaagaac ttaaaatgct taacatattc attgaggttt
     5521 gtaccacata atgagatacg agataatttg ttgggagtaa aatcaaaaaa aagattaaat
     5581 atttaaaata atcactatat tttttcatta tttgttcctt tttgtattta agttcttatg
     5641 aaaaactagg gtattttgaa tagaaaatat caaaataccc tagttttatt aaggaatatt
     5701 accaccaaca agtgcttcca tattgtacag caccagtgat tgcaccacca atagctccga
     5761 atttcgcacc tggaatagca ccaataccac ctgcaaatga accaacaatt gccccaccag
     5821 ctgctccacc taatgcacta ccaatggtat tttttgagaa atcacgccag ttacaagcag
     5881 caccagaaac ttgttaaatt tcattcaaag tcaaaacttg cataatattc acctttgtta
     5941 agtgtggttg agaaaaaagt aaattttcat cgttactcat tatttgcaat catgcatata
     6001 ctgtaaccaa gcactatatt cagttggatc atagtttttc ctactgggtt gtctgatatg
     6061 actattggca ctattggcac aattgggatt agcaacttta ttgtgagtac ctaatggatt
     6121 gatatttcct gtaaagtggc tattgcatgc gcttaatgag cataaattta ttaatgtcag
     6181 aaaggataaa acaaaaaatc ttaccatttt ttactaaaac caaaagtagg tttcgaaaac
     6241 tgtacaccaa gaccttgtac atatccaaat tgcttgctaa cagaaaactc tactccacta
     6301 ttgttagggg aataggaagc accaatttta ccaacagcaa ccatatcttt tttacctagg
     6361 ttgccgccac cctgtgcaga gatatggaat ttgccacctg acacttcaac taattcagag
     6421 gtgtgtaact ctttcatgat tttctcctgt aaagaagtat ggttggaaat gtaaatcaga
     6481 attaaccttt cttaagttta atttaatact tttttttgca agtattttat tttaaattaa
     6541 atcaatattt taaaaatatt tacttgtata ctgaaaaaga aactcctaat ggtggagtgg
     6601 ggcttttgtt taagtaagca ttgaaaaaat ttaaatttcc cattatgatt aacttaatgc
     6661 aagagctttt tcaggacgta caaaccagcg aacgggcgtt ctgatgtggg caggttggtt
     6721 gtcgctaacc aatttcagta gctcaatcgt cagattggta tccgacccta tcaagctaag
     6781 gagaggctgc ggctactaca caaagcacct gaaattattg ggtgcttttg tctttttatg
     6841 gcattgtacc aatttgtgta ccacgagggc ttgttcgccc aaacgcggta acaactgaat
     6901 acacactttc cccctcaact tctttgcgtt ctaaaacgac tgtcccaatc cgagaataga
     6961 caatcatagg acgatgattg ccccttacat cactaaattc actaaaaatt tttgcgcctg
     7021 attgaaaaat catttccaca taaaagggtt acacctgttt catcgtggct taatccaaac
     7081 cgcgttaaat ctgctttgtg ttctgccaaa atgtgaatca caccaaagcc gtgattaacc
     7141 tttttgtgtt cgccgtgttt taaaacaatt tgacctgccg agaagtctgc tctattgttc
     7201 ggacaatagg gggaatatac caattacttt aactacatca ttttttgaca tttttcttgc
     7261 ctttcaattt aaaatccaaa atcatactgc cataatttag caatccaaaa aaatttaggc
     7321 agcagtcatg gtttcgtatt ttagaaaacg aaacttggtt tggtgttttg aagcatttca
     7381 gtacacaaac atggaatttt aaattgtctt gtgttgcctt gatttaggat gttccagttt
     7441 tttaataaat agtttggggc tgtcctagat aactagga
//
LOCUS       DAWFIT010000097         7360 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFIT010000097 DAWFIT010000000
VERSION     DAWFIT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.097"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WGQ85_001855"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGQ85_001855"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGQ85_001856"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WGQ85_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0751220.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WGQ85_001857"
     CDS             complement(1737..2630)
                     /locus_tag="WGQ85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0751221.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WGQ85_001858"
     CDS             2972..3421
                     /locus_tag="WGQ85_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0751222.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WGQ85_001859"
     CDS             3495..3803
                     /locus_tag="WGQ85_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751223.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="WGQ85_001860"
     CDS             3919..4761
                     /locus_tag="WGQ85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0751224.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="WGQ85_001861"
     CDS             4751..5497
                     /locus_tag="WGQ85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0751225.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="WGQ85_001862"
     CDS             5519..6106
                     /locus_tag="WGQ85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0751226.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="WGQ85_001863"
     CDS             6112..6291
                     /locus_tag="WGQ85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0751227.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="WGQ85_001864"
     CDS             6532..6972
                     /locus_tag="WGQ85_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751228.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1886 a         1950 c         1669 g         1855 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcctgtcgga tgcacaccgg acaggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttgccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acagcctaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aagtcgccgt ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtgcg caagtcttta atgcgtgagg agtccaagac gtacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggcggctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgataac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tagaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaactgga tggcacgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgacaatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcggcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggtggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccagcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcacgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc ctttaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgcgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgcggttgc acgtgaggcc gattgcggca ttcatctgga cgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctattggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga ggtcgccaaa attgccgact atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaatctccgc ccaataccaa caccgtttcg cccaagccat
     4801 acgcgagggt gaagccgccg acggactgcc gcaagagcgc ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttt
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctggagataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc gcactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatcgg
     6361 cagttcatcc ataatacacg cttcaaaagc agcatattcc tccatacgga atatataaat
     6421 acgcaaaaca cgaaggctgc atcaatttgc tatatttgct ttatttacct tatttcacag
     6481 acggcgctac ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttat tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaataa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgttaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 caattgtttg atacaaaaaa cacggaaatt tccacacaaa actacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cgggacatta accgaaaaac aatatgaaac cgtatgcgga
     6961 aaaaaactct gattgcaact tatacacaaa cttacccaca aaccttatca taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttctc cgaaaagtta
     7081 tcctcgaagt tatccacatt atttttttaa accggcttcc atccgaaata tagtggatta
     7141 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     7201 caacgccgta ctggtttaaa tttaatccac tatataaact cgctatacaa tttcactatc
     7261 caaacgtaaa ttgttccatt gatacacaaa actgcttacc cccataattt tgataaagca
     7321 tttcttacat tcccggctcc gtcccgtaac caacacagcg
//
LOCUS       DAWFIT010000098         7343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFIT010000098 DAWFIT010000000
VERSION     DAWFIT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.098"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(314..636)
                     /locus_tag="WGQ85_001865"
                     /pseudo
     CDS             complement(314..636)
                     /locus_tag="WGQ85_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(623..970)
                     /locus_tag="WGQ85_001866"
     CDS             complement(623..970)
                     /locus_tag="WGQ85_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0751229.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQKVEVAKGVITAQMASSNVNNE
                     IKGKKLSLWAKRQNGSVKWFCGQPVQRDKADADADADAVTADTAAANKKIDTNHSHEP
                     TSRHSRESGNDGI"
     gene            complement(1031..1333)
                     /locus_tag="WGQ85_001867"
                     /pseudo
     CDS             complement(1031..1333)
                     /locus_tag="WGQ85_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1362..1691)
                     /locus_tag="WGQ85_001868"
     CDS             complement(1362..1691)
                     /locus_tag="WGQ85_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0751230.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1769..1987)
                     /locus_tag="WGQ85_001869"
     CDS             complement(1769..1987)
                     /locus_tag="WGQ85_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0751231.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            2109..3062
                     /locus_tag="WGQ85_001870"
     CDS             2109..3062
                     /locus_tag="WGQ85_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0751232.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCYYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3137..5194)
                     /gene="metG"
                     /locus_tag="WGQ85_001871"
     CDS             complement(3137..5194)
                     /gene="metG"
                     /locus_tag="WGQ85_001871"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0751233.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(5310..7148)
                     /gene="glmS"
                     /locus_tag="WGQ85_001872"
     CDS             complement(5310..7148)
                     /gene="glmS"
                     /locus_tag="WGQ85_001872"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0751234.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEAFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1458 a         1768 c         2191 g         1926 t
ORIGIN      
        1 ctagccgaat tactttattt tttgatacgc aaccggccgg ttgccgtcat tcccgcgcag
       61 gcgggaatcc agtccgcttg ttcggtacat gagagggcgg attgcgcgcc tgtcagataa
      121 accccgtgtt taaacgggcg gcaatgaggt acgcgcagag ccttgaagcg caatcgatat
      181 attattttca gccaaaacgg acgcccccgc ttgccttgca aacccttaaa aaggaagcca
      241 cccggattaa tccgagtggc cgtggaaaat cacttaccgc ttgatttatt taaaatttat
      301 ggtataattt gccttatccg gtagatgaat catcgcggca ggttgacggc aggtgcttgg
      361 tgttgatttt gtcgtcggtg ccgttggcgg tggcggcttt gacgtcgtct ttggctttgt
      421 cggcgcgcgc aaccggctgt ccgcagaacc attttaccga accgttttga cgcttggccc
      481 acagggagag ttttttgcct ttgatttcgt tgtttacgcc gcttgaaagc attgtggcgg
      541 taatgacgcc gtttgcgact ttaacttcct taacatattt gcctttgatg tcggtggggg
      601 ggatgccacg ccggcggcat cattaaatcc cgtcattccc actttcgcgg gaatgacggg
      661 atgtaggttc gtgggaatga ttggtgtcga tcttcttgtt ggcggcggcg gtgtcggcgg
      721 taacggcgtc ggcgtcggcg tcggcgtcgg ctttgtcgcg ctgaaccggc tgtccgcaga
      781 accattttac cgaaccgttt tgacgcttgg cccacaggga gagttttttg cctttgattt
      841 cattgtttac gttgcttgaa gccatttggg cggtaatgac gccttttgcg acttcaactt
      901 tctgaacata tttgcctttg atgtcggagg gggaggatgc cacgccggcg gcatcattaa
      961 atcccgtcat tcccactttc gtgggaatga cgggattaaa gtttcaaaat ttattctaaa
     1021 taactgaaac tcaacgaact agattcccgc ttttgcggga atgacgaagt ttttctgcca
     1081 tttgccgtga ttcgggcaat actcggtaac ggctgatttt ttgaaagtgt ttgaaatcgg
     1141 cgcgtggtgt ttttgtgcaa acggcagatg acgcatcgcg gcaggttgac ggcaggtgct
     1201 tggtgttgat gttgtcggcg gcggtgcgcg taaccggctg tccgcagaac cattttaccg
     1261 aaccgtcttg acgcttggcc cacagggaga gttttttgtc tttgatttcg ttgtttacgt
     1321 tgcttgaagc cattatgtca gacggtattg cccgggcagc tttattcgta cactttcagc
     1381 agctcgactt caaatatcaa agtggcgtgc gggggaatca cgccgcccgc gccgtgtgcg
     1441 ccgtagccca tttccgaagg gatggtcagc ttgcgtttgc cgccttcctt cattccgccg
     1501 aagccttcgt cccagccttt gatgacttgt ccgacaccga gcgtgatggt cagcggctgg
     1561 cggcggtcga ggctggagtc gaatttggtt ccgttttcca gccaacctgt gtaatgcacg
     1621 gtaatctctt tgcctttaac tgcttctttt ccgaagcctt cttgcaagtc ttcaataatc
     1681 aggccgccca tatttgtcct ttcattgctt gttggtcaaa acggcaaggg taacataccg
     1741 tccgtcgaag tcaaatgccg ctcaaacgtc agctgcatcg gtgcagctga aacggctgtg
     1801 tttgttggac tgttttattt ttttcgtaaa ggttccatgc tttttcatgg aaatagaaaa
     1861 cgacggtgtt gattaggggc tcgaccagcg cgaccgcccc cgatacgcct atactgcccg
     1921 tcagtacata ggttacactg aaggcggtgc tgaaatgcag tgcggcaaaa gtcagggttt
     1981 taagcatcat cctctcccga attggacatt gacggaggaa tgataaaggt tatcataagg
     2041 ttgcgcggtt taaatttgct atttgttgtt agtgtaaata aatcattttt caaatgggga
     2101 tagaaattat gaatcataaa aagatcgttg ttttggatgc ggatactttg cccggccggg
     2161 tttttcattt tgattttccg cacgagcttg cggtttacgg tacgacaggt gcggatgaaa
     2221 cggcagaacg ggtgcgcgat gcacatattg tcattactaa caaagtgatg atttctgccg
     2281 atattattgc ggctaatccg cagttggagc tgattgccgt cagtgcgacc ggtgtgaaca
     2341 atgtcgatat tggggcggcg aaggcggcgg gcgttgcggt atgcaatgtc cgcgcctacg
     2401 gaaacgaatc ggttgcggaa cacgccttta tgctgatgat tgcgttgatg cggaatttgc
     2461 ccgcctatca gcgtgatgtt gcggcaggat tatgggagaa gtcgccgttt ttctgctatt
     2521 acggtgcgcc gattcgggat ttgaacggta aaacgctggc ggttttcgga cgcggcaata
     2581 tcggacggac gcttgccgga tacgcgcagg cgttcggtat gggggtggtg tttgccgaac
     2641 acaaacacgc gcccgacgtg cgcgaaggct atgtttcctt tgaagatgcg gtacagactg
     2701 ccgacgtgtt gtcgctgcac tgtccgctga acgcccaaac tgaaaatatg ataggcgaaa
     2761 acgaattaca gcagatgaag cccggtgcgg ttttgattaa ttgcgggcgc ggcgggctgg
     2821 tggatgaaaa cgcgctgctt gccgcactca aatacgggca gatcggcggg gcgggtgtcg
     2881 atgttttgac gaatgagccg cccaaaaacg gcaatccctt gctgaatgca cgattaccca
     2941 atctgattgt tacgccgcat accgcgtggg caagtcgtga ggctttggac aggctgtttg
     3001 atatattgtt ggcgaacatt cacgcctttg tgaaaggaga ggcgcaaaac cgcgtggttt
     3061 gagcttgtcg ggatgacgga aaacaatgcc gtctgaacgc ccaaagggtt cagacggcat
     3121 tttttgagat tcctgtttaa ccgactttgt cgcccggctg cgcgcctgta tccacatcta
     3181 agagcttcag tttcccgtct gccgtggcgg cactcaaaat catgccttcg gatacgccga
     3241 attttgccat tttgcgcggg gcgaagttgg cgacggcgat gaccattctt ccgtttaact
     3301 cggcagggtt ggggtaaaac gcggcgatgc cggagaagat gatacgtttt tcaaaaccga
     3361 aatcgaggtc gaatttcaaa agtttggtgc tgccttcgac ggcttcgcag ttcaatactt
     3421 tggcgacgcg catatcgatt ttcatgaagt cgtcgaaact tgcctgttcg gcgacttttt
     3481 cgtatttgcc ctcttcggcg gcaggtgcgg ctgcggcggc gatgctttgt ttgttggctt
     3541 cgattaaatc gtccacttgt ttttgctcca ctcgttgcat taaatgtttg tatttgttga
     3601 tggcgtgttt gcccaaggtt tcgcgtgtat ttgcccaagt gatggcttcc aaattcagga
     3661 atttggcggc gtttgcggcg gtttgcggca agacgggggc gaggtaggcg gtcaacatgg
     3721 tgaaggcgtt gatgagctcg ctgcacactt cgtgcaggcg ttcgtcttga ccttcctgtt
     3781 tcgccagttc ccaaggtttg ttggcatcaa cgtattcgtt cacggcatct gccaatgcca
     3841 tgatatcgcg cagggcgcgg gcgtattcgc ggttttcgta ttgctcggcg atggtgtcgc
     3901 tttcggcggc aagttttgcc agcaatgcgc tgtcggcaac gtctttcaga cggccttcaa
     3961 agcgtttggc gataaaacct gaggcgcggg cggcgatgtt gacgtatttg ccgacgaggt
     4021 cgctgtttac gcgcgaaata aagtcttgca ggttcaaatc gatgtcttcg attttgctgt
     4081 tgagttttgc ggcgatgtag tagcgcatcc actcggggtt taaaccttgt tccagatagg
     4141 atttggcggt gataaacgtg ccgcgcgatt tggacatttt ttgtccgtct acggtcaaaa
     4201 agccgtgtgc gtacacgccg gtcggggcgc ggtggccgga gaaatgcagc atggcaggcc
     4261 agaacagggc gtggaaatag aggatgtctt tgccgatgaa gtggtacatc tcggtttggc
     4321 tgtcggcttt gaagtattcg tcaaaatcga cgccgatgcg gtcgcacagg tttttaaacg
     4381 acgccatgta gccgacgggc gcgtccagcc agacgtagaa gtatttgccc ggcgcgtcgg
     4441 ggatttcaaa accgaaatac ggcgcgtcgc gggaaatatc ccagtcggac agggtggttt
     4501 cttcaccttc gcccagccat tctttcattt tgttgagggc ttcggcttgc agatggggct
     4561 tgccgtcgtg cgggttgttg ccggaagtcc atgctttgag gaagtcggcg cattctccca
     4621 gtttgaagaa gaagtgttcg gattcgcgca attcgggttt cgcgccggaa acggcggaat
     4681 acgggttaat cagttcggtc ggggaatagg tcgtgccgca gacttcgcag ttgtcgccgt
     4741 attggtcttg ggcgtggcat ttcgggcatt cgcctttaac gaagcggtcg ggcaggaaca
     4801 tttgtttttc ggggtcgaaa agctgctcga tgacgcggct ttcgattttg ccgttggctt
     4861 tcagtgcgcg gtaaatgtct tgggaaaact gtttgttttc aggggaatgg gtgctgtaat
     4921 aattgtcgta gccgatgaaa aaaccggtaa agtcggcgag gtgttcttcg cgcactttgg
     4981 caatcatgtc ttcgggcgca atgccttgtt tttgcgcggc aagcatcacg ggcgtgccgt
     5041 gggtgtcgtc ggcgcagcag tagtggcact cgtggccgcg cagtttttga aagcgcaccc
     5101 aaacgtcggt ttggatgtgt tcgaccatgt ggccgaggtg gatgctgccg ttggcatagg
     5161 gcagggcgga ggtaacgagg attttgcgtg tcatattgtg ctttgcaaac aatgggtaaa
     5221 ggcggattat accgcaaatc aaacggggaa atgccgtctg aagcctgaaa aatcgggctt
     5281 cagacggcat ttttgccaac cggcgggatt tattcgacgg ttacggattt cgccagattg
     5341 cgcggcttgt ctacatccgt gccgcgcgcg agggcggtgt ggtaggaaag gagctgcacg
     5401 gggatggtat gcacgacggg ggacagttcg ccgacatggc gcggcgcacg gataacgtgc
     5461 acgccttcgg ttgcattaaa attgctgtcg aggtcggcaa agacgaaaag ttcgccgccg
     5521 cgtgcgccga cttcctgcat attggctttg actttgtcca acaggctgtc gttgggcgcg
     5581 atgacgacga cgggcatgtt ttcgtccacc aaggcaagcg gcccgtgctt cagttcgccg
     5641 gcaggatagg cttcggcgtg gatgtaggtg atttccttca gctttaacgc gccttcgagg
     5701 gcaatcgggt aatggatgcc gcgccctaaa aacagcgcgc tggttttctt ggcaaactgt
     5761 tgcgcccagg cggcaatttg aggttcgagg tttaaagcgt gctgcacgct gccgggaagc
     5821 tggcggagtt cttcggtgta acgtgcttca tcttcgccgg ataccaaacc gcgcactttc
     5881 gccagcgtta ccgccaaacc gaacagcgcg accagctgcg tggtaaacgc cttggtcgag
     5941 gcgacgccga tttccgcacc ggcacgggta taaagcacga ggctgctttc gcgcggcagg
     6001 gcggattcca tcacgttgca gatggagagg ctgtggcggt gtcccaaaga tttggcgtat
     6061 ttcaacgcct ccatcgtgtc cagcgtttcg ccggattggg aaatggtaat gaccagttgg
     6121 tccggatcgg caatcacgct gcggtatcgg tattcgctgg cgatttcgac gtcggtcgga
     6181 attttggcga tggattcgag ccagtatttt gcggtcagcg cggcgtaata ggacgtgccg
     6241 caggcaagga ttttgacgct gcggatgctt tcaaacacgc ttttggcgtc tttgccgaag
     6301 ttttcgggaa tgaagccgcc gtcgaggaaa gcctccgccg tgtctgcaat cgcgcgcggc
     6361 tgctcatgga tttccttttg cataaagtgg ctgtacggtc ccagttccaa agaggcgagc
     6421 gagagttcgg ataccttgac tttgcgttcg gcaggcaggc cgtttttatc ggtcagcctt
     6481 ttgatgccgt ctgaagccag cagcgcgatg tcgccgtctt cgaggtacgc cacgcggcgc
     6541 gtaaaggcga tgacggcgga tacgtccgaa gcgataaagg tttcatcgtc gcccaaagcg
     6601 accaaaagcg ggcagcccat acgcgccaca actaattcat caggcttgtc ttgggcaata
     6661 accgcgatgg cgtatgcgcc gtggaaacgt ttgaccgctt cttgtaccgc ttcaaacagc
     6721 ctgccgccgt tttgcgcgta ttcgtgattg atgctgtgtg cgatgacttc ggtatccgtt
     6781 tgcgattcaa aacggtatcc caaaccttcc aaacgtttgc gttcgctttc aaagttttcg
     6841 atgatgccgt tgtgtacgac cgcaatcata ccgccgctga tgtgcgggtg ggcgttcggc
     6901 tcagtaacgc cgccgtgtgt cgcccaacgc gtatgtccga tgccgatgcc gccgctgatg
     6961 cctttttcgc gtgccgcgtc ctccataagc tgcacgcgtc cgacgcggcg cacgcgtttg
     7021 attttgccgt cggtgttgac ggcaatgcct gatgagtcat acccccggta ttcgaggcgt
     7081 ttgaggccgt cggtcagaaa atcgacgacg ttgtgatggg cgcggatggc gccgacgata
     7141 ccgcacataa ctgttcctta gtatcaggtt gaaaaaaaac aggcgcggac ggctcccgtg
     7201 ccgcaccttc cgcttcggat tataaaccgc ctagtgtcgg aaaacagcaa aatgccgtct
     7261 gaaggcttgg gcttgctcaa aaaaggaggg atttccccgc ctgaccggga tgggcgttca
     7321 gacggcatta cctgccgccg gtt
//
LOCUS       DAWFIT010000099         7336 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFIT010000099 DAWFIT010000000
VERSION     DAWFIT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.099"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..163
                     /locus_tag="WGQ85_001873"
     CDS             <1..163
                     /locus_tag="WGQ85_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751235.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            200..412
                     /locus_tag="WGQ85_001874"
     CDS             200..412
                     /locus_tag="WGQ85_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0751236.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WGQ85_001875"
     CDS             560..2143
                     /locus_tag="WGQ85_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0751237.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="WGQ85_001876"
     CDS             2227..3324
                     /locus_tag="WGQ85_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0751238.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFLWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="WGQ85_001877"
     CDS             3438..4553
                     /locus_tag="WGQ85_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0751239.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="WGQ85_001878"
     CDS             4678..5133
                     /locus_tag="WGQ85_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0751240.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="WGQ85_001879"
     CDS             5204..5680
                     /locus_tag="WGQ85_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0751241.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5668..6594
                     /locus_tag="WGQ85_001880"
     CDS             5668..6594
                     /locus_tag="WGQ85_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0751242.1"
                     /translation="MSEMKKNKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6659..7216
                     /locus_tag="WGQ85_001881"
     CDS             6659..7216
                     /locus_tag="WGQ85_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0751243.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1921 a         1809 c         1917 g         1689 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agacggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atctgcttga
     1141 agaacccttg gaagcgcgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaaaggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgttaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcaggggat gattatattg ccaaagaatt ttatagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctag aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 caccacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac actgcgaaaa gcggtacacg
     2221 acacatatga accatacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
     2581 gagggattgt ttgcctgttt gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt acattcctcc gatttgagtg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgc
     2761 cctttgcttt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cttgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttccgacggc agccacacgc gccagcgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acaggtttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt tttgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtcttcaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatac cttgcccgct tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgtgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga cagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggccgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accggaatct gtaaaaaatg tcagaaatga aaaaaaacaa agtccacctg
     5701 atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat ggcgcaaaaa
     5761 gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgagatcgg tattgacggc
     5821 gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg tttgtgttgc
     5881 accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc caagcccgac
     5941 cgcctgatga ttgaagcaag cggactggct cacgcagcca gcgtcatcga cgaactcaaa
     6001 gccaaaccgc tggacagcct tttggaaatc ggcgccgtct ttaccgtcgt caatccgcgc
     6061 cagttcatca accccgatta cgcgcaacag gcgttgtata aagaccaaat cggcatctgc
     6121 gacgtattgg tcgccagcaa aaccgattta tgcaccaccg aacagcttgc cgaatttcac
     6181 gacaaagcgg caaaactgtt cccgcccaaa gccaaagtgg tcgaagtcca aaacgcacaa
     6241 ctcgacatcc ggtggctcga tattcccgtc atcgaaaaat cacgctaccg cctcaaagcc
     6301 ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc cggacgcgat
     6361 ttcgacggcg aaaagctgac caactttttc aacgacctgc ccaaaatgac cgaaggactc
     6421 gtccgcgcca aaggcgtgtt tcaagtattg ggaacgtggg tgtggctcaa ctgggtagac
     6481 ggacagtggg gcgcgaacca agtctcatgg cggcgcgatt cgcgttttga attgattgcc
     6541 aaatcgtttg atgcggattt aatcgaacaa aagctcaaag acgcattgga atagcgttca
     6601 cgcgccttca gacggcatat gaaatgaaaa tgccgtctga aggtctgagg aggacgggat
     6661 gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttccc tgccgtctat
     6721 ggcggcaacc catgttttga tggaaaccga tatgggcaat atccgtttgg ttttggacga
     6781 atccaaagca cccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag gcttttacga
     6841 caatacgatt tttcaccgcg tcatcggcgg cttcgttatc caaggcggcg gattgaccga
     6901 ggacttggca caaaaggcaa gcgataaggc cgttgccaac gaatccggca acggcttgaa
     6961 aaacactgtc ggcaccatcg ccatggcgcg gacgacagac cccgattccg ccaccagcca
     7021 attctttatc aatctggcgg acaatgattc actcaactac aaaaacggac aatacggcta
     7081 taccgttttc ggcagggtcg aaagcggaat ggacaccgtt tccaaaatcg cccgcgtcaa
     7141 aaccgccacg cgcggctttt atcaaaacgt acccgtacag cccgtcaaaa tccgtcgcgt
     7201 tgttgtcggg cagtaacacg cagacagatg ttcagacggc gtcgcccgtt tcacaaaaaa
     7261 cgccgtctga agtgaaaaaa tattttaaaa cagatagttg atattgacaa attcaaaccg
     7321 aggattttaa aatgct
//
LOCUS       DAWFIT010000100         7203 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFIT010000100 DAWFIT010000000
VERSION     DAWFIT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.100"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(85..954)
                     /locus_tag="WGQ85_001882"
     CDS             complement(85..954)
                     /locus_tag="WGQ85_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0751244.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGQ85_001883"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WGQ85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0751245.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WGQ85_001884"
     CDS             complement(1963..3060)
                     /locus_tag="WGQ85_001884"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0751246.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGQ85_001885"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WGQ85_001885"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0751247.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAIDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WGQ85_001886"
     CDS             complement(4392..5291)
                     /locus_tag="WGQ85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0751248.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WGQ85_001887"
     CDS             complement(5395..6048)
                     /locus_tag="WGQ85_001887"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0751249.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WGQ85_001888"
     CDS             complement(6127..6780)
                     /locus_tag="WGQ85_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0751250.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7057..7141
                     /locus_tag="WGQ85_001889"
     tRNA            7057..7141
                     /locus_tag="WGQ85_001889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Leu,seq:caa)
BASE COUNT         1476 a         1715 c         2124 g         1888 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagtt cgtccgaacc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atgtatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgttccc aaaggcgagg aaaagcgggc ggtgcggctg gtgggcaaca catggtttgg
     3301 gccggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc
     3361 gtggcggatt tttttcgccc attcctgcgg gttttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atctatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgcct ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgctttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctggatcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatga atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttt gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aacaggcgcc gacggtgcag atggcatacg
     6121 gttttatcag agcagggtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggt tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agccgacgcg gatgcacttg aagaagcgac ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggtaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtctgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct
     6961 atttaaaatc aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga
     7021 atttcaaaag ccattataat gacgacttct tcaccagccc aggtggcgaa attggtagac
     7081 gcaggggact caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc
     7141 acaaccgctt ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataagac
     7201 taa
//
LOCUS       DAWFIT010000101         7197 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFIT010000101 DAWFIT010000000
VERSION     DAWFIT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.101"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            326..1771
                     /gene="zwf"
                     /locus_tag="WGQ85_001890"
     CDS             326..1771
                     /gene="zwf"
                     /locus_tag="WGQ85_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0751251.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVKRFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2051..2746
                     /gene="pgl"
                     /locus_tag="WGQ85_001891"
     CDS             2051..2746
                     /gene="pgl"
                     /locus_tag="WGQ85_001891"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0751252.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            2727..3713
                     /locus_tag="WGQ85_001892"
     CDS             2727..3713
                     /locus_tag="WGQ85_001892"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0751253.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            3763..4611
                     /gene="hexR"
                     /locus_tag="WGQ85_001893"
     CDS             3763..4611
                     /gene="hexR"
                     /locus_tag="WGQ85_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0751254.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            4732..6378
                     /gene="pgi"
                     /locus_tag="WGQ85_001894"
     CDS             4732..6378
                     /gene="pgi"
                     /locus_tag="WGQ85_001894"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0751255.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            6392..6943
                     /locus_tag="WGQ85_001895"
     CDS             6392..6943
                     /locus_tag="WGQ85_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0751256.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1884 a         1896 c         1900 g         1517 t
ORIGIN      
        1 aaaaggtata ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
       61 tgttacaaaa aaaatcagac atcgaaaaga aatttgattt aaatcgcaga atctttcgat
      121 tgttgtaacg gaatagggga aacctgcccg atttttggac taggtcaaat aaattgtaaa
      181 aatgggaata gacaaaaagt gtagcggtgt gtagtattac tactcattat gccgcctgcc
      241 tttgtttccg atattgcgat gcagcgtgat ggggaaaaca ggtttcagac ggcatcgaaa
      301 aattctttcc tgacgagata atgcgatgag tacacaaaca aattttgatt tggtgttgtt
      361 cggtgcgacc ggcgatttgg caatgcgcaa acttttgccc tgcctgtatc aggcgcacgt
      421 ggccggtttg cttcatcccg aaggtcgtat tttgggtgtg agccgcagcg aattggatac
      481 cgaaggtttt ctggcgaagg tggaaaccag ttccaaaatc catgtgaaag aaaatttttc
      541 cgatgaggct tgggcatcgt ttgtcaaacg tttcgcatat ctcaaagtcg atgttacgca
      601 gccggacgat tttgccgctt tgggcgattt ggtcaaggcg cgcaaagaaa ccgacaatgt
      661 cgtcatctat ctttccaccg cgccgaaatt cttcgcgcaa acatgtgaaa accttgccaa
      721 aatcggtttg aacgccgaca acgtgcgtgt ggttttggaa aaaccgttgg gtacggattt
      781 ggcttcttcc caacaaatca ataccgatgt tgcgcgttat ttcaaagaag ggcaaatcta
      841 ccgtatcgac cattatttag gtaaagaaag cctgcaaaac ctgctggcct tgcgttttgc
      901 caatgtgatg ttcgagccgc tttggaacaa taaatacatc gaaagcgtgc agcttaccat
      961 cgccgagcag ttgggcgtgg aagaacgcgg cgagttttac gacattaccg gcgcgttgcg
     1021 cgatatggtg caaaaccact tgatgcaaat gctgtgcatg actgcgatgg aagcccccgc
     1081 cagcttggat gccgacgcgg tgcgcgatga aaaagtcaaa gtcatcaagt cattgaagcc
     1141 gctgaccgtc gaatctgtca atgaaaatgt cgtgcgcgga caatataccg ccgccaaagg
     1201 catgaacggc tatcttgaag aaatcaacgt tccgcaagac agctttaccg aaacctacgt
     1261 cgccattaaa gccgaaatcg aaaacgaacg ctggaagggc gttcccttct acctgcgtac
     1321 cggcaagcgc atggcgggca aagtggcgga aatcgttttg aacttcaaag atttgaacag
     1381 ccatattttt gaaggcagtc gcaccgcgcc caaccggctc gttatcgagt tgcaaccata
     1441 tgaatccgtg cgcctctata cgcagatgaa aaccccgggg gcaggaaata aggtcgaaac
     1501 cgtgccgctg gcaaccgatt tgggcaaagc attggaaagc cgccgcgcgg aagcttacga
     1561 gcgcctgctg ctggatgtga ttaacggcaa actcgctttg tttaaccgcc gcgacgaact
     1621 tgaagccgcg tgggaatatg tgatgccgat tttggaaaac tggacaaata acaccacgcc
     1681 gccgcacggc tacggcgcac actcgtgggg gcctgaagcc gcgcgcgaac tattggcgcg
     1741 cgacggacac aagtggcacg aagagcagta atacaataat gcgttcagac ggcatggggt
     1801 ttgaaatgcc gtctgaacgt aagtaaagca gtatagtgga ttaacaaaaa tcaggacaag
     1861 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     1921 aaatttaatc cactataaaa agcagtcccg atatttggta tcaaaagcat aaacaactgt
     1981 tcggccgaca tattgctcaa cccgtttcca taacggaata tgccgtctga aataaaaaaa
     2041 ggacacaaat atgtttgttt ggcacgaata cgaaaatgcg gcagaagcgg cgcagtcttt
     2101 ggctgacgca gtggcggatg ctttgcaggg cgcactggac gagaagggcg gtgcggtgtt
     2161 ggcagtttcc ggcggacgtt cgccgattgc atttttcaac gccctgtcgc aaaaagattt
     2221 ggattggaaa aacgtcggca tcaccttggc agatgaacgc atcgtgccga ccgtccacgc
     2281 cgacagcaat accggtttgg tgcgcgaata cctgttgaag aacaaagcgg aagcggcaat
     2341 gtggattcct atggtggaag acggaaaaac tgaaaccgaa ttacatcccg atgctgttgt
     2401 cgattatgca ctgaaacatt acaaacagcc cgatgttttg gttttgggta tgggaaacga
     2461 cgggcatacg gcttcgattt tcccgaaagc tccgcagttt cagacggcaa tcgacggttc
     2521 ggcaggtgtc gcgctggtgc ataccacgcc cgttaccgcg ccgcacgagc gcgtcagtat
     2581 gaccttggat gcgattgccc atacggggca tgtgtttttg gcgatacggg gcgaagagaa
     2641 aaaagccgtg ttcgaccaag ccgcacaagg cgaaaaccgc gaatatccga tcaacctcgt
     2701 tttgaaccat caaggagtga actgccatgt cttctacgcc gaataaacaa gccggatatc
     2761 cccgactggt cgccgatatc ggcgggacga atgcacgctt tgcgctggaa accgcgccgc
     2821 gcgtcattga aaaagccgcc gtgcttccgt gtaaagacta cgatacggtt accgatgcgg
     2881 tgcgtgccta tctgaatcaa agcggtgcaa cagccgtacg gcacgcggca tttgccatcg
     2941 ccaacccgat tttgggcgac tgggtgcaga tgaccaacca ccattgggcg ttttccatcg
     3001 aaaccacccg tcagactttg gggctggaca ccctcatcct tttgaacgac tttaccgcgc
     3061 aggcattggc ggtaacgcag acttcaagca aagacctgat gcaggtaggc gggcaaaagc
     3121 ctgtcgaatt tgcccccaaa gccgttatcg gccccggtac cggcctgggc gtgagcggat
     3181 tggtgcacag ccacgcaggc tgggtggctt tggcgggcga gggcgggcat accagtttcc
     3241 cgccgtttga cgatatggaa gtgctgattt ggcagtacgc caaaaacaaa tacggccatg
     3301 tttccgccga acgctttttg agcggcgcgg gcttgagctt ggtttacgag gctttggctg
     3361 caaaacagaa agccaaaccc gccaaactga tgccgtctga aatcacggaa aaggctttga
     3421 gcggcgcgtc gcctttgtgc cgtcagactt tggacatctt ctgcgccatg ctcggcacgg
     3481 ttgcttccaa cctcgccctg acgctgggcg cgcgcggcgg cgtgtacctg tgcggcggca
     3541 ttattccccg cgtgttggaa tatttcaaaa cttccccgtt ccgcagccgt ttcgagaaca
     3601 agggcaggtt tgaagcatat cttgccgcga ttcccgtgta tgtcgtcttg agcgagtttc
     3661 ccggaatttc cggtgcggct gcggctctcg acaaccattt gagaaacgtt taaccacagc
     3721 ggctccttgc agcggggctg cattatcgaa gggcatatca ttatgttaag caaaatcagc
     3781 gaatcactgg caaacctttc cggtgcggaa cgcaaagtcg ccgaatgtgc attggcggaa
     3841 cccaaatggt tcgtccatgc cgccgttgcc gaaattgccg aacgtgcgtc cgtcagccag
     3901 ccgaccgtca tccgattctg ccgcagcttg ggttataaag ggctgcccga gttcaagctc
     3961 gccttgtccg ccagcatcgg tcatgagggt atgccctatg tccacgaaga actcaacgcc
     4021 gacgacgata tggcaagcgt ggtcgagaaa gtgttgggca atgccgccgc ctcgctgttg
     4081 ggcgaacgcc gcttcctgaa agagtcggag ctggaaaacg ccattgccac gctgatgcac
     4141 gcccgtcgcg tcgagtttta cggtgtcggc aattccggca ttgtggcaca ggacgcgcag
     4201 cataaatttt tccgtttcgg catgtccacc gtcgcctatg tcgatacgca cacgcagctg
     4261 atggcggcat ctgttttgag cgatcaggat gttttggttg ccatttccaa cacgggttcg
     4321 tctatcgaac ttttggatgc ggtcagcatc gccaaagaaa acggcgcgtc tgtcatcgca
     4381 ctgacccgca acgattcgcc tctggcgcaa cttgccgact gcgtgttgag cgttgccaca
     4441 caggaaaatg ccgaactcta cacgcccatg gtttcccgcc tcttgcagct tgccgtcatc
     4501 gacattctcg ccatcggact tgccctgcgc ttgggcgatg ctgccagcct gcaattgcag
     4561 aaaagcaaaa aaagcataca caacaagcac atcgattacg acaaagattg accttcagac
     4621 ggcatcccac aaatgccgtc tgaaatgccg aacaacggtc gtcggcggct tgcggcagtt
     4681 tccggcagcc ttttcatccc acaacaaaaa acctcattca ggagcatata gatgaaacac
     4741 tttcacgact tacccgcatg gtcgaaattg tggaaccact ttgacgacag caaaacattg
     4801 catatgcgcg aaatgttcga gcaagatccg cagcgtgcgg aacgctactg gctgcaagtc
     4861 ggcggactga cgctggacta ctccaaaaac cgcatcaacg acgaaaccat gtcgcttttg
     4921 ttcgagttag cgcgtgaagc aggcgtgccg gagcggatgc ggcagatgtt ccacggcgaa
     4981 aaaatcaata ccaccgaaaa ccgcgccgtc ctgcatgtcg cccttcgcaa ccgcaccaat
     5041 tcgccgattg tggttgacgg tgaagatgtg atgcccaaag tcaaccgcgt tttgcaacgt
     5101 atgggcgaat ttgcacacga agtccgcagc ggaagctggc tgggctatac caaccaagtc
     5161 attaccgacg ttgtcaacat cggcatcggc ggatcggatt tgggtccgct gatgatgtgt
     5221 accgcgctca aacctttcgg tcatccgcgc ctcaatatgc acttcgtctc caacgtggac
     5281 ggctcgcaac tgcgcgacgt attgtccaaa gtccaccccg aaaccacgct gttcatcatc
     5341 gcctccaaaa catttaccac gcaggaaacg ctgaccaacg cgctgaccgc gcgcgaatgg
     5401 tttttgaatc atgcgggcga cgaagaagcc gttgccaaac acttcgccgc cgtttccacc
     5461 aatcaaaaag ccgtcgccga attcggcatc gacaccgcca atatgtttga attttgggat
     5521 tgggtcggcg gtcggtacag cctgtggtcc gccatcggat tgccgattat gctgtatctc
     5581 ggcgaagaaa acttcatcga aatgctcaac ggcgcgcacc tgatggacca acacttcatc
     5641 aacacaccgc tcgagcgcaa cctgcccgtc attctcgccc tcatcggcat ctggtatatc
     5701 aactactacg gcggcggcag ccacgtcatc gcgccttacg accaacattt gcaccgcctg
     5761 cccaaattca tccagcagct cgatatggaa agtaacggca aacaggttac gttggacggc
     5821 aaagcagtcg gacacgaaac ctcgccgatt atctggggcg aaacgggcat taacggccag
     5881 cacgccttct tccaactgct gcaccaaggc acgcacatta cccccatcga cctgattgcc
     5941 tcgcttgaaa aacgcagcaa cctgcccgga caccacgaaa tcctgcttgc caatgtcttc
     6001 gcccaagcag aagcctttat gcgcggcaaa acccccgacg aagtccgcgc cgaactcaaa
     6061 gcgcagggta tggatgaggc gcgcatcgaa gagctggtcc cgcacaaaac cttctccggc
     6121 aaccgcccga ccaacctcat tctcatggac aaggtcaacc cgcgcaatat gggcagcctg
     6181 attgccatgt acgaacacaa aaccttcgta caaggcatca tttggggcat caacagcttc
     6241 gaccagtggg gcgtggaact cggcaaacaa ctggctaaaa ccattttggg cgaactgacc
     6301 ggcgaaaccg ggccgcaaaa gcacgacagt tcgaccgaac gcctgatcaa cctctacctg
     6361 cagaccaacc gcaaataaaa cctgcggaaa aatgccgtct gaacgccgac cgtttcagac
     6421 ggcattttta tcgaacagga aaaccgtcgg taaacttgca gagcgtgtgc aatcccgata
     6481 tgatggtttg cataaattta aacatatatg ttccgcagct atggcactga ttaaagagcc
     6541 gttggacaaa gcgaaacaaa ggaacgaaga acttgaagcg gcagaagaag cggcggcgca
     6601 ggaggcattg ggtcgggagc aggaagtcga ccgcgtatcc gaatgggaag aacgctacaa
     6661 gctgtcgcgc agcgagttcg agcagttctg gaaaggattg cctcaaaccg tacagaataa
     6721 gctgcaagcc tcacagaaaa catggaaaag cgggatggat aaaatctgtg ccaacaatgc
     6781 gaaagctgaa ggtaaaacgc caaacggcat aaaattcagc gaactggcat gcaaaacggc
     6841 gaaaaccgaa gcacgcttgg aagagctgca caaccgtaaa aaagccctta tcgacgaaat
     6901 ggccagggaa gcggacaaga aagaactgtc aaagcggctc tgaacagcgc ggttcaggca
     6961 ctgcccgccg atattgccga aaccgtcatg cccgagtacc gcaactgaca aaacggtttg
     7021 aatgccaaat gcgccgacgg cgacgaatac ggcgtggcac aatctgactg ccgtaccaga
     7081 gaaatcaatg cgaaaaccaa agaaatccaa ggttatctga ttgactgaaa cttggatgcg
     7141 ggaatgtcgg cggcttttgc gtttttgtcg tttttatagt gaattaaatt taaaccg
//
LOCUS       DAWFIT010000102         7158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFIT010000102 DAWFIT010000000
VERSION     DAWFIT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.102"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGQ85_001896"
     CDS             complement(111..1457)
                     /gene="mnmE"
                     /locus_tag="WGQ85_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0751257.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1588..1743
                     /locus_tag="WGQ85_001897"
     CDS             1588..1743
                     /locus_tag="WGQ85_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751258.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1740..2171
                     /locus_tag="WGQ85_001898"
     CDS             1740..2171
                     /locus_tag="WGQ85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0751259.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2305..6654
                     /gene="app"
                     /locus_tag="WGQ85_001899"
     CDS             2305..6654
                     /gene="app"
                     /locus_tag="WGQ85_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0751260.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
     gene            complement(6970..>7158)
                     /locus_tag="WGQ85_001900"
     CDS             complement(6970..>7158)
                     /locus_tag="WGQ85_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751261.1"
                     /translation="PYWFKFNPLYVVEQSFGTLHRKFRYARAAYFGLSKVSAQSHLKA
                     MCLNLLKAANRLSAPAAA"
BASE COUNT         1880 a         2088 c         1752 g         1438 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaaccgaac ggatcggatt cccgttttta
       61 tgggataacg gaatgttcag ccggacaccg ccacggtgct ttcgatccgt ttattttccg
      121 atacaaaacc tcgaaaaaat cacgccgagc aggtcgtccg ccgtaaactc gcccgtgatt
      181 tcgccgcatg cgacctgcgc caagcgcaag tgttcggcaa acagctcgat ttgatggttg
      241 ccgcacaatg ccgccagcga caattcttcc tgcgctgctt tgagtgcgtt gacgtgccgc
      301 gtccgcgcca aaaacagccc ttcgccctcg ccctgccatc cggcctcgcg caacaacgtc
      361 cgtttcagcg cgtccaagcc gtcgccggtt ttcgccgaca gcgcgatgac ggtttccgcg
      421 cccgtaccga acccgcctgc cgcgtgtgcg tgcaaatcgg atttgctgtg gatttcgatg
      481 cgtttcaact ccggcggcaa cgcgtccaaa atcgcccgtg tcttgtcgtt cacgccttcg
      541 cgcggatcga ccaacaccag cgcgacatcg gcttcggata cggctttgcg gctgcgttcg
      601 atgccgatgc gttcgaccac gtcgtccgtc tcgcgcaaac ctgccgtatc gacaatatgc
      661 accggaacgc cgtcaatcag gatacgttcc ctgaccgcgt cgcgcgtcgt tccggcaata
      721 tcggtaacaa tcgccacttc gtcgcccgcc agcgcgttca acaggctgga tttgccgaca
      781 ttcggcgcgc cgaccaatac gacattcaga ccttcgcgca aaatcgcgcc ctgctgcgcg
      841 ttggcaagca catcatccac ggcgcggcgc aagccgtcca gtttgccgcg tgcgtctgcc
      901 gcttcgagaa aatcaatgtc ttcctcggga aaatctaacg tcgcttcgac cagcatccgc
      961 aaggtaatca agtcttcgac cagaccgtgt atccgccgcg aaaaatcgcc tttgagcgag
     1021 cgcaacgcca gacgcgccgc cgaacggctg gatgcgtcaa tcaaatccgc cacgccttcc
     1081 gcctgtgcca agtccagttt gtcgtttaaa aacgcacgct tcgtaaactc gcccggttcg
     1141 gcaaggcgcg cgcccaattc caaacagcgg ttcagcagca tatccatcac taccggcccg
     1201 ccgtgtccct gaagctcgat aacatcttcg cccgtaaaac ttgccggtgc ggcaaaaaaa
     1261 agcaaaagcc cgctgtcgat tgcctgaccg tccgcgtccg taaaatcagc ataggttgcc
     1321 acgcgcggct tgggcgtttt cccacacaaa gcctctgcca tcggcagcag gtttttcccc
     1381 gatatgcgta tcacgcccac gccgccgcgc ccgggtgcgg tagcgactgc cgcaatcgtt
     1441 ggaacgttat ccgacataaa acccccgaaa attcaaaaca gccgcgatta tagcaaatgc
     1501 cgtctgaagt ccgacggttt ggctttcaga cggtataaaa ccgcaaaaat gcttgataaa
     1561 tccgtccgcc tgacctaata taaccatatg gaaaaacgaa acacatacgc cctcctgctc
     1621 ggtataggct cgctgttggg tctgttccat cccgcaaaaa ccgccatccg ccccaatccc
     1681 gccgacgatc tcaaaaacat cggcggcgat tttcaacgcg ccatagagaa agcgcgaaaa
     1741 tgaccgaaaa cgcacaggac aaggcgcggc aggctgtcga aaccgtcgtc aaatccccgg
     1801 agcttgtcga gcaaatcctg tccgacgagt acgtgcaaat aatgatagcc cggcgtttcc
     1861 attcgggacc gttgccgccg ccgtccgact tggcgcaata caacgacatt atcagcaacg
     1921 gggcagaccg cattatggca atggcggaaa aagaacaagc cgtccggcac gaaaccatac
     1981 ggcaagacca aaccttcaac aggcgcgggc aactgtacgg cttcatcagc gtcatcctga
     2041 tactgctttt tgccgtcttc ctcgtatgga gcggctaccc cgcaaccgcc gcctcccttg
     2101 ccggcggcac agtggttgcc ttggcgggtg ctttcgtgat tggaagaagc cgagaccaag
     2161 gcaaaaatta atgacaaatc ctagggcgtg cttcatatcc gcccgaacgc cgaaccgcac
     2221 atataggcac atcccgcgcg ccgcccggaa gcggaagccg cgccctccca aacaaacccg
     2281 aatcccgtca gataaggaaa aataatgaaa acaaccgaca aacggacaac cgaaacacac
     2341 cgcaaagccc cgaaaaccgg ccgcatccgc ttctcgcctg cttacttagc catatgcctg
     2401 tcgttcggca ttcttcccca agcctgggcg ggacacactt atttcggcat caactaccaa
     2461 tactatcgcg actttgccga aaataaaggc aagtttgcag tcggggcgaa agatattgag
     2521 gtttacaaca aaaaagggga gttggtcggc aaatcaatga caaaagcccc gatgattgat
     2581 ttttctgtgg tgtcgcgtaa cggcgtggcg gcattggtgg gcgatcaata tattgtgagc
     2641 gtggcacata acggcggcta taacaacgtt gattttggtg cggaaggaag aaatcccgat
     2701 cagcaccgtt tttcttacca aattgtgaaa agaaataatt ataagcctga caattcacac
     2761 ccttacaacg gcgattacca tatgccgcgt ttgcataaat ttgtcacaga tgcagaacct
     2821 gtcgaaatga cgagtgacat gagggggaat acctattccg ataaagaaaa atatcccgag
     2881 cgtgtccgca tcggctcagg acaccactat tggcgttatg atgatgacaa acacggcgat
     2941 ttatcctact ccggcgcatg gttaattggc ggcaatacac atatgcaggg ttggggaaat
     3001 aatggcgtag ttagtttgag cggcgatgtg cgccatgcca acgactatgg ccctatgccg
     3061 attgcaggtg cggcaggcga cagcggttcg ccaatgttta tttatgacaa aacaaacaat
     3121 aaatggctgc tcaacggagt tttacaaacc ggctaccctt attccggcag ggaaaacggt
     3181 ttccagctga tacgcaaaga ttggttctac gatgacattt acagaggcga tacacatacc
     3241 gtcttttttg aaccgcgcag taacggacat ttttccttta catccaacaa caacggtacg
     3301 ggtacggtaa cagaaaccaa cgaaaaggtt tccaatccaa agcttaaagt acagacagtc
     3361 cgactgtttg acgaatcttt gaatgaaact gataaagaac cagtttacgc ggcagggggt
     3421 gttaatcagt accgtccaag gttaaacaac ggtgaaaacc tttcttttat cgattacggc
     3481 aacggcaaac tcatcttatc aaacaacatc aaccaaggcg cgggcggttt gtattttgaa
     3541 ggtgatttta cggtctcgcc tgaaaacaac gaaacgtggc aaggcgcggg cgttcatatc
     3601 agtgaagaca gtaccgttac ttggaaagta aacggcgtgg caaacgaccg cctgtccaaa
     3661 atcggcaaag gcacgctgca cgttcaagcc aaaggggaaa accaaggctc gatcagcgtg
     3721 ggcgacggta cagtcatttt ggatcagcag gcagacgata aaggcaaaaa acaagccttt
     3781 agtgaaatcg gcttggtcag cggcaggggt acggtgcaac tgaatgccga taatcagttc
     3841 aaccccgaca aactctattt cggctttcgc ggcggacgtt tggatttaaa cgggcattcg
     3901 ctttcgttcc accgtattca aaataccgat gaaggggcga tgattgtcaa tcataatgcc
     3961 acaacaacat ccaccgttac cattacaggg aatgaaagta ttacacaacc gagtggtaag
     4021 aatatcaata gacttaatta cagcaaagaa attgcctaca acggttggtt tggcgagaaa
     4081 gatacgacca aaacgaacgg gcggctcaac cttgtttacc agcccgccgc agaagaccgc
     4141 accctgctgc tttccggcgg aacaaattta aacggcaaca tcacgcaaac aaacggcaaa
     4201 ctgtttttca gcggcagacc gacaccgcac gcctacaatc atttaggaag cgggtggtca
     4261 aaaatggaag gtatcccaca aggagaaatc gtgtgggaca acgactggat caaccgcacg
     4321 tttaaagcgg aaaatttcca tattcagggc gggcaggcgg tgatttcccg caatgttgcc
     4381 aaagtggaag gcgattggca tttgagcaat cacgcccaag cagtttttgg tgtcgcaccg
     4441 catcaaagcc atacaatctg tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa
     4501 aaaaccatta ccgacgataa agtgattgct tcattgacta agaccgacat cagcggcaat
     4561 gtcagccttg ccgatcacgc tcatttaaat ctcacagggc ttgccacact caacggcaat
     4621 cttagtgcaa atggcgatac acgttataca gtcagccaca acgccaccca aaacggcaac
     4681 cttagcctcg tgggcaatgc ccaagcaaca tttaatcaag ccacattaaa cggcaacaca
     4741 tcggcttcgg gcaatgcttc atttaatcta agcaacaacg ccgcacaaaa cggcagtctg
     4801 acgctttccg acaacgctaa ggcaaacgta agccattccg cactcaacgg caatgtctcc
     4861 ctagccgata aggcagtatt ccattttgaa aacagccgct ttaccggaca actcagcggc
     4921 agcaaggata cagcattaca cttaaaagac agcgaatgga cgctgccgtc aggcacggaa
     4981 ttaggcaatt taaaccttga caacgccacc attacactca attccgccta tcgccacgat
     5041 gctgcaggcg cgcaaaccgg cagtgtgtca gacacgccgc gccgccgttc gcgccgttcc
     5101 ctattatccg ttacaccgcc aacttcggta gaatcccgtt tcaacacgct gacggtaaac
     5161 ggcaaattga acggtcaagg aacattccgc tttatgtcgg aactcttcgg ctaccgaagc
     5221 gacaaattga agctggcgga aagttccgaa ggcacttaca ccttggcggt caacaatacc
     5281 ggcaacgaac ccgtaagcct cgatcaattg acggtagtgg aagggaaaga caacaaaccg
     5341 ctgtccgaaa accttaattt caccctgcaa aacgaacacg tcgatgccgg cgcgtggcgt
     5401 taccaactca tccgcaaaga cggcgagttc cgcctgcata atccggtcaa agaacaagag
     5461 ctttccgaca aactcggcaa ggcagaagcc aaaaaacagg cggaaaaaga caacgcgcaa
     5521 agccttgacg cgctgattgc ggccgggcgc gatgccgccg aaaagacaga aagcgttgcc
     5581 gaaccggccc ggcaggcagg cggggaaaat gtcggcatta tgcaggcgga ggaagagaaa
     5641 aaacgggtgc aggcggataa agacagcgcc ttggcgaaac agcgcgaagc ggaaacccgg
     5701 ccggctacca ccgccttccc ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccgcag
     5761 ccccaaccgc aacctcaacc ccaaccgcag cgcgacctga tcagccgtta tgccaatagc
     5821 ggtttgagtg aattttccgc cacgctcaac agcgttttcg ccgtacagga cgaattggac
     5881 cgcgtgtttg ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa
     5941 cactaccgtt cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt
     6001 atgcagaaaa acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa
     6061 aacaccttcg acgacggcat cggcaactcg gcacggcttg cccacggcgc cgttttcggg
     6121 caatacggca tcggcaggtt cgacatcggc atcagcacgg gcgcgggttt tagcagcggc
     6181 agtctttcag acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta cggcattcag
     6241 gcacgatacc gcgccggttt cggcggattc ggcatcgaac cgtacatcgg cgcaacgcgc
     6301 tatttcgtcc aaaaagcgga ttaccgctac gaaaacgtca atatcgccac ccccggtctt
     6361 gcgttcaacc gctaccgcgc gggcattaag gcagattatt cattcaaacc ggcgcaacac
     6421 atttccatca cgccttattt gagcctgtcc tataccgatg ccgcttcggg caaagtccga
     6481 acacgcgtca ataccgccgt attggctcag gatttcggca aaacccgcag tgcggaatgg
     6541 ggcgtaaacg ccgaaatcaa aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc
     6601 ccgcaactgg aagcgcaaca cagcgcgggc atcaaattag gctaccgctg gtaaccgccg
     6661 gatatgccga aaggggtctg acgatgccac cgtgcgctgt caaacccttt ttctgccgcc
     6721 gccttgtgcc tgctccattg tctgataacc gtcaaattga tttttagccc atgtttggtg
     6781 ggtcggggaa atctatatct tcgtccgtgc cgaaatagtc tgagaccttt gcaaaattcc
     6841 tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc cccaaatacc
     6901 tcctaattct acccaaatac ccccttaatc ctccacggac acccgataat caggcatccg
     6961 ggctgctttt taggcggcag cgggcgcact tagcctgttg gccgctttca aaaggttcaa
     7021 acacatcgcc ttcagatggc tttgtgcact cactttgctc agcccgaaat aggctgcccg
     7081 cgcatagcgg aatttacggt gcagcgtacc gaagctctgt tcgaccacat atagtggatt
     7141 aaatttaaac cagtacgg
//
LOCUS       DAWFIT010000103         6962 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFIT010000103 DAWFIT010000000
VERSION     DAWFIT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.103"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..284
                     /locus_tag="WGQ85_001901"
     CDS             <1..284
                     /locus_tag="WGQ85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751262.1"
                     /translation="ENRQHLKEHQLQDGIMRKACRNRPLTEAQTKRNRYLSKTRYVVE
                     QSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(623..1183)
                     /locus_tag="WGQ85_001902"
     CDS             complement(623..1183)
                     /locus_tag="WGQ85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0751263.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1600..2640)
                     /gene="mutY"
                     /locus_tag="WGQ85_001903"
     CDS             complement(1600..2640)
                     /gene="mutY"
                     /locus_tag="WGQ85_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0751264.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2803..3907
                     /locus_tag="WGQ85_001904"
                     /pseudo
     CDS             2803..3907
                     /locus_tag="WGQ85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3930..4148)
                     /locus_tag="WGQ85_001905"
     CDS             complement(3930..4148)
                     /locus_tag="WGQ85_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751265.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(4163..4801)
                     /locus_tag="WGQ85_001906"
     CDS             complement(4163..4801)
                     /locus_tag="WGQ85_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0751266.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4982..6817)
                     /gene="edd"
                     /locus_tag="WGQ85_001907"
     CDS             complement(4982..6817)
                     /gene="edd"
                     /locus_tag="WGQ85_001907"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0751267.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIHSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1579 a         1664 c         1956 g         1763 t
ORIGIN      
        1 cggaaaaccg gcaacatctg aaagaacatc agttgcagga cggcattatg cgcaaagcct
       61 gccgcaaccg tccgctgacg gaagcgcaaa ccaaacgcaa ccgatatttg tcgaagaccc
      121 gttatgtggt cgaacaaagc ttcggtacgc tgcaccgcaa attccgctat gcgcgggcag
      181 cctatttcgg actgattaaa gtgagtgcgc aaagccatct gaaggcgatg tgtttgaacc
      241 tgttgaaagc ggccaacagg ctaagtgcgc ccgctgccgc ctaaaaggca gcccggatgc
      301 ctgattatcg ggtatccggg caggattaag ggggtatttg ggtaaaatta ggaggtattt
      361 ggggcgaaaa cagccgaaaa cctgtgttgg gatttcggct gtcgggaggg aaaggaattt
      421 tgcaaaggtc tcaaatagta cggaatcgat tcacttggtg cttcagcacc ttagagaatc
      481 gttctctttg agctaaggcg agccaacgtt gtaccggttt aaatttaatt cactatactg
      541 ctgaagtcgt gaattaaatg ctaaatatct agcaaaaaag tgcggtcaaa atttaccgca
      601 cttttcgttt cgaacaatcg aattatttaa tcacgccaca tgccatacgt gggccgccac
      661 cgccaagtgg agctggatga tcggagtgat tatcaccacc cgtgtggatc ataatagagt
      721 gaccgcgaac atcatctaaa tgtttaagac gtggtgctaa aacaggattt gttgctgtgc
      781 catcatgcaa tacagttaat gcaggtaaat cacctaagtg tgcatcatct tgccatgggt
      841 aaccatgttg ttttgcacct ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca
      901 atttaccttc tttttctttt ggctcacagc ttgggttttc atggatgtgg aaaccatgta
      961 agccttcgct taatccttgt aaatcagggg taaacacaag accatagtta gattcagtaa
     1021 tagtcactgt acccacatct ttgttaccgt ttactggatc aagttgttgc actttcactt
     1081 caatagaagc acctttaggt atcgtattat gctcgtgtgc ttgcgcaaca ctaactgaac
     1141 atactgcact aaccgctagt gctaataagg ttttcatatt cattgcaatc tcctttattg
     1201 cagtaaaaac tcgcgactat tttagtcaat tttgttttaa aagccgataa atttttacga
     1261 aaattttaca ttaattgtta tttgcagaaa attagaaccc gttatcacgt cttggataaa
     1321 tgccaaaacc attgtgattc agagaattaa aaagaaaaat ttcggaatcc tcatcctttt
     1381 ctgaaacctg attgcggtat ccatggcttt gtctttgtca tttaagtttg tgttcatgtt
     1441 tttgaccttc tttgcttttt aaatcctttt taaattgccg acttcttccg ttccaaatgt
     1501 cccatttgaa cattatcccg ccgcctaaaa tacgacccaa attcatcggt tgttcttgct
     1561 gcgatacctg caaacaacag ttcaatgggt ttattttgtt tattctaacc tattaccgtt
     1621 taaataaatt tccaaaggct tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca
     1681 aatgccgtct gaagggcttt cagacggcat ttgtccttca aacggcgtaa tcatcagcaa
     1741 ccggtgcgtc agccggtggg tcagggcggt ttgttcgtcc atatctgcca tggtcaggga
     1801 gaatttggcg gcaaagtcgg aaagcccgtt caaactttca aaacacggca cgcaatacag
     1861 cccgccccaa atgcctttgg cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg
     1921 gacaatcagc cagtaaagcg gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag
     1981 ctcggcggtg cggttttgct ttttcgcttc gcagatgtcc gccatcgggc attggtggca
     2041 caagggtttc gtccgtttgc acacggtcgc gcccaaatcc atcaaacctt gtgtataggc
     2101 aggcatatcg gcgttttcag acggcagcag gctttcggca agtgtccaga gcgagttttc
     2161 aaattttttg tcctgcggat tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac
     2221 gttgccgtcc aaaatggttt cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt
     2281 gcttctgcct acgccgcaga gggtttccaa gtctttgcgc tccgacggaa acgtgccgcc
     2341 gaattgttcg acgacttgtt gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc
     2401 caagcccgcc cacaacgaca acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt
     2461 cgggaatttt tctaagaaac gcggatagta gtccaacacg gtggcgacct gcgtttgctg
     2521 gagcatgatt tcggaaagcc agacgcaata agggtttttg acctgccaag ggaggtggtg
     2581 gcgaccgtgt tgtttttgcc agcggatgag ccgttcggag aaggggatgg gtgtgttcat
     2641 taatatcaat cggtggtttt atttatattt aaaacagtat gttattgtat aacattgtga
     2701 aaataattct tattgactta ttttttgtag gggcatataa ctcatataag gaaactttat
     2761 tgcggtattg aaattattta tcaacaagca aggagtatca gcatgaaagc aatggtttat
     2821 cacggcgcaa acgacatccg ttttgaagaa aaaccccgtc cgcagattat cgatccgacc
     2881 gatgcggtgg tgaaaatcgt caaaaccacg atttgcggta ccgatttggg tatttggaaa
     2941 ggcaaaaaaa cccgaagtcg ccgacggccg tattctcggt catgaaggca tcggtattgt
     3001 agaagaagtc ggcgaggctg taaaaaacat caaagtcggc gataaagtca ttatttcatg
     3061 cgtcagcaaa tgctgtactt gcgacaactg caaaatccaa ctttattcgc attgccgcaa
     3121 cggcggctgg attttgggct acatgattga cggcacgcag gcagaatacg tccgcacgca
     3181 ggcagaatac gtccgcacgc aggcagaata cgtccgcacg caggcagaat acgtccgcac
     3241 gccttatgcc gacaacagcc tcgtgccgct gcccgacaac gtcaacgaag aagtcgccct
     3301 gttgttgagc gacgctttgc cgaccgcaca cgaaatcggc gtgcaatacg gcgatgtcaa
     3361 acccggcgac accgtattca tcgcaggtgc cgctcctgtc ggtatgtccg ccttgccgac
     3421 cgcccaactg tacagccccg ccgccatcat tgtttgcgat atggacgaaa accgtttgaa
     3481 actggcgaaa gagttgggcg caacccatac cattaaccct gcttccggcg atgtaagcaa
     3541 gcaagttgcc gctatcgtcg gcgaagacgg cgtggactgc gcgatcgaag ccgtcggtat
     3601 ccctgcaact tggaatatgt gccaagacat tgtgaaaccc ggcggtcata tcgccgttgt
     3661 cggcgtacac ggtcaatccg ttgatttcaa actggaaaaa ctgtggatta aaaagcttgc
     3721 catcactacc ggcctggtaa acgccaatac caccgaaatg ctgatgaagg caatttccag
     3781 cagctccgtc gattacacca aaatgttgac ccaccgtttc aaattcagcg aattggaaaa
     3841 agcctacgac gtgttcaaac acgccgccga aaatcaagcg atgaaagtgg ttttggaagc
     3901 ggattaatcg gcgttgaatc agttgttttt taagcggatg ccgtctgaat gcgtttcaga
     3961 cggcattttt tcagccgcgt agtatgggct gtccggctgc tgtcgaatga actgaacgtt
     4021 atttttatta tgcggaaggc ggatgggcat tttaggggca gtatgtcgga gtggtgctgc
     4081 cgtgtatgcc gactgcgccg ccaagcggca aaatgccgtc tgagactgga aagggcttca
     4141 gacggcattt ttacgatgcg attcaggctt tgggtttcaa tgccgccgct tctttggcga
     4201 gacgggtgat ggtgtcccag tctttgtttt tcacggcttc tttcggcgtc agccatgagc
     4261 cgccgacgca caggacgttg ggcagtgcca agtaatcggg cgcggtggcg aggctgatgc
     4321 cgcctgtcgg gcagaagcga acatcggcgt aagggccgta cagggctttg agcatggctt
     4381 tgccgccaac gacttcggca gggaagagtt tgagggtgtc gatgccgtgt tccaaagcca
     4441 gttggatttc gcccggggtg gcaacaccgg gaatcagggg gatgccgctg ttgtggccgg
     4501 ctttggcgag ggattcgtgc aaacccgggc tgatggcgaa aaccgcgcct gcgtcttcga
     4561 cggctttgag ctgttcaggg ttggtaaccg tacccgcgcc gacgatggcg ttgggcactt
     4621 ctttggcaat caggcggatg gcatcgaggc cgacaggggt gcgcagggtg atttcgaggg
     4681 tggggatgcc gccttcgaca agggcgtggg acaaatcgac ggcggtgctt aagtcgtcaa
     4741 tcgccattac cggcacaact gcgccggcgg tcaaaatttc gcggggggtc agtttggaca
     4801 tttcggttct ccgggtggaa tggggtattt tattaagatg ggacaggtcg taggtcggat
     4861 tcaagaaacc gacatcttgg acggctgtgt cggattcgag aatccaacct acgtctatct
     4921 taaaacactt gaataatttt tcagacggcc ttcggtctga aaaggccgtc tgaaacgcgc
     4981 atcaggcaaa ttcgccgccg aaactcatgg caccggtttc cgcgctgctg gtcatgctgc
     5041 ggaagttggc gaagagttcg cggccgcagc cttgttggtt cgcgcccaag tcgatgcttt
     5101 cgacttcgcg ggcattccat tcggtttcgt taatcaggac gttgagttcg ccgctaacgg
     5161 agtcgaagcg gatcaggtcg ccggtacgga ttttggcgat gttgccgccc atcagggctt
     5221 cgggtgtcat gtggatggag gccggaactt tgccagatgc gccggacata cgaccgtcgg
     5281 tcagcagtgc cactttgaag ccgcggtctt gcaggatgcc caaaggcggg gtcagtttgt
     5341 gcaattcggg cataccgttg gcacgcgggc cttggtagcg gacgacgcag acgaaatcgc
     5401 gttccaactc gccgcgttca aatgccgcca acacttcgcg ttggtcgttg aacacgatgg
     5461 caggcgcttc aataatgcgg cagccttcgc gcacggcgga cactttaatc acgccgcgtc
     5521 cgatgttgcc tttcatcagg cgcagaccgc cgtcggggga gaacgggttg ccggctttgc
     5581 gcaggatgtc gtcgttgccg ctggtttcgg gggcttcgcg ccattcgagt ttgccgtcga
     5641 taaggaaagg ctctttggtg tagtggcgca taccgtgtcc gacaacggta tcgacatcgt
     5701 cgtgcaacag gcctgcattc agcaattcgc ggataacgaa aggtaggccg ccggcagcgg
     5761 taaagtggtt tacgtcggct ttgccgttgg ggtacacacg gatcagcaat gggatgatgg
     5821 aagaaatttc gtcaaagtcg tcccagttca aaatcacgcc ggcggcgcgt gccatggcaa
     5881 cgaggtgcat ggtgtggttg gttgagccgc cggttgccat caggccgatt agggcgttga
     5941 tgaaagattt ttcggtcaac atttcaccca atggtttaat cgtgccgttt ttgatgccgc
     6001 gcgcgaggtg tccggcggca tagcgggtca gggcttcacg caggtcggtg taagggtgga
     6061 cgaaggcggc ggcaggcagg tgtacgccca tcatttccat catcatttgg ttggagtttg
     6121 ccgtgccgta gaaagtgcag gtgcccgggc tgtggtaaga acccatttcg cttttcaaaa
     6181 gttcgtctcg tccgactttg ccttcggcga aaagctggcg ggtgcgggct ttttctttgt
     6241 tgccgatgcc gctggacatc gggcctgcgg ggacgaagat acccggaata tgaccgaacg
     6301 aaagcgcgcc tatcatcaaa cccggcacga ttttgtcgca tacgcccata aacaggctgc
     6361 cgtcaaacat ttgatgcgac agaccgatag cggtactcat ggcgatcacg tcgcgggaaa
     6421 acagcgacaa ttccataccg gcgtagcctt gcgtgatgcc gtcgcacatg gcgggtgtgc
     6481 cgccggcgac ttgggcggtc gcgccgtttt tctgcgcttc gtctttaatt tggtcgggga
     6541 agtctttaaa cggctggtgt gcggaaacca tgtcgttgta ggcggtgatg atgcctaagt
     6601 tggggacggt ttcctggagc atttcgattt tgatgctttt gggcatagag gcatagccgt
     6661 gcgccaagtt ggagcagccg agctggttgc gctctaagcg tcccatttgt ttggcactgt
     6721 ggattttcgc cagatatttt tcacgcgtcg gacggctgcg ttcaataatg cgcccggtga
     6781 tttcggcgag tttggagtgg ataggagtag tgttcacggt cggtctcctg tcggaacggg
     6841 gggtattggg gatttgtttg tgcgtttctg attttttgta ttgggtgtgg ggcatttggc
     6901 gtttcagacg acctttcaaa ccttacaagg ggtcgtctga aaacctcact atagtggatt
     6961 aa
//
LOCUS       DAWFIT010000104         6905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFIT010000104 DAWFIT010000000
VERSION     DAWFIT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.104"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            975..1421
                     /gene="yciA"
                     /locus_tag="WGQ85_001908"
     CDS             975..1421
                     /gene="yciA"
                     /locus_tag="WGQ85_001908"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0751268.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(1472..2293)
                     /locus_tag="WGQ85_001909"
     CDS             complement(1472..2293)
                     /locus_tag="WGQ85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0751269.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADES
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2412..2870
                     /locus_tag="WGQ85_001910"
     CDS             2412..2870
                     /locus_tag="WGQ85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0751270.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(3034..4149)
                     /gene="lst"
                     /locus_tag="WGQ85_001911"
     CDS             complement(3034..4149)
                     /gene="lst"
                     /locus_tag="WGQ85_001911"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0751271.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            4250..4354
                     /locus_tag="WGQ85_001912"
                     /pseudo
     CDS             4250..4354
                     /locus_tag="WGQ85_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4582..6807
                     /locus_tag="WGQ85_001913"
     CDS             4582..6807
                     /locus_tag="WGQ85_001913"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0751272.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALST
                     L"
BASE COUNT         1832 a         2032 c         1552 g         1489 t
ORIGIN      
        1 cgacgcggcg ggaatctatc ggaaatgact gaaaccccga gattctagat tcccacttcc
       61 gtgggaatga cgtggtgcag gtttccgtat ggatggattc gtcattcccg cgcaggcggg
      121 aatccagacc cccgacgcgg cgggaatcta tcggaaatga ctgaaacccc gcgttctaga
      181 ttcccacttc cgtgggaatg acggttcagt tgcgctccga caacaccgta atctcaaaac
      241 ccgtccgaca acaccgcaat cttgaaattc gtcattcccg cacaggcggg aatccagacc
      301 tgtccgcaca gaaacttatc ggataaaaac agttgcccaa accccgcgtt ctagattccc
      361 actttcgtgg gaatgacgtg gtgcaggttc catatggatg gattcgtcat tcccgcgcag
      421 gcgggaatcc aaacccccga cgcggcggga atctatcgga aatgactgaa accccgagat
      481 tctagattcc cactttcgtg ggaatgacgg ttcagttgcg ttccgacaac accgtaatct
      541 tgaaatccgt ccgacaacac cgtaatctta aaacccgtca ttcccgcgca ggcgggaatc
      601 cagacctccg acgcggcggg aatctatcgg aaatgactga accccccgag attctagatt
      661 cccacttccg tgggaatgac gtggtgcagg tttccgtatg gatggattcg tcattcccgc
      721 gcaggcggga atctagacct gtccgcacag aaacttatcg gataaaaaca gttgctcaaa
      781 cccctcgttc tagattccca cttccgtggg aatgacggtt cagttgctac ggccccgccc
      841 tacaatacgc cttgcgaatc tgtcccacgc ccgcccttgt atgtctcaaa tgggttaaac
      901 tacgcacttc ccgacttccc gtttcgggca ggggtgcagg ttcgatttat ctttcaataa
      961 aacaaggaaa cattatgcag cacgaagaag gcaaccgcca acgccctcaa ggcgaactgc
     1021 tcctgcgtac cgtcgctatg ccgcgcgata ccaatcccaa ccaagacatt ttcggcggct
     1081 ggattatgtc gcagatggat ttgggcggcg gcatattggc ggcggaaatc gcgcggggac
     1141 gcatcgttac cgtcgccgtt caggaaatga actttatccg cccggtcaag gtcggcaacg
     1201 tcgtctgctg ctacgggcat tgcgtccgcg tgggcaatac ttccctccag cttaaagtcg
     1261 aagtctgggt gaaaactttg atgaacgatt gcgttaccga agaccgccac ctcgtaaccg
     1321 aagccgtgtt cacttatgtc gccatcgatg cggaaggcaa tccgcgcccg attccgaaag
     1381 aaggcaaccc catattggcg ggcttattgc ctactccgta aaaatacccg taaaaatgcc
     1441 gtctgaaact gtcttcagac ggcattccgc atcatttgaa ccaagatctg atgcgggaaa
     1501 acagcgacat actctgtttt tcaaagtctt cccgcacttc cggcacggct ttttttatca
     1561 ctttcattcc tgcaaaagtc tgcgccacgg gcataatttc aatcgtgttc acattcatat
     1621 gcgccggccg gcggtacagc cacaatgcgg tttccgcaat atcttcgggg cggataaatt
     1681 ccacaccctc atacacgcct gctgccttct cgtcatcgcc tttgaagcgc acgttggaaa
     1741 actccgtatt gccgcacaaa cccggctcga tattggtaac gcggatgttc ttatccgcca
     1801 actccgcgcg caaattcagg ctgaactggc gcacaaacgc cttggtcgcc ccgtaaacgt
     1861 tgctgccggc ataagcataa ttgcctgcaa tcgaacccaa attcatcaca taaccgccgc
     1921 cgcgttccac catttgcggc aaaattttgc gcgtcaggaa cgtcaaaccc aaaacattgg
     1981 tttgaatcat cgtttcccaa tcttcaaaat ccgccttgtc cgccgtgtcc agacccaaag
     2041 ccagcccggc attgttgatg aggcagtcga tgtcggaaaa ttcatcgggg atgccgttta
     2101 aggcgttttc caccgactcg cgacgcgaca cgtccatttc caaagggtaa aacaaagcac
     2161 ccgattcatc cgccaaagcc tgaagcctgt ccgcgcggcg cgccgcaccg ataacgcggt
     2221 atcccgcccc gacaaacgca cggcacatcg cttcgccgaa tcctgccgaa gcaccggtaa
     2281 ttaaaacagc cattgttttt cctttctttc tcgtttccca aacctgtttc aggtatcata
     2341 acacccttca gacggcatcg cgccgactga aaatgacaac aaccatacca ccacgacagg
     2401 acacattcaa tatgaaaacc caaatcagcc ttgctgctgc cgccatcaca ctgctccttt
     2461 ccgcctgcgg aggcagcgga ataccttccc aacccaaagg cgaaatttcc gaaaaccgca
     2521 ccgccgcgtt caaatccatg atgcccgaat tcacacgcat gggcaaaatg gtcaaagacg
     2581 aagaacctta cgatgtcgaa aaattcaaac aggctgccgc ggcgtttgcc gaaagcagca
     2641 agaaaccgtt cacacttttt gagtccgatc cgcaaggcaa cggccgcgcc ctgcccgccg
     2701 tttggtcgga tggtgcaaaa tttgaagccg aaaaaacaaa attcgccgcc gccgtcgaaa
     2761 aactcaacgc cgcagcacaa accggcaaac tggatgaaat caaagccgcc tacggcgaaa
     2821 ccggcgcaag ctgcaaatcc tgccacgaca gtttccgcgc gcccgaataa catccaaaac
     2881 aaaacaatgc cgtctgaagc cttcagacgg cattttcttt gccatttcag cacacgcaaa
     2941 gcaatcagaa attatcttca attcaaaaaa tacgccatat cctgattttt gccttactta
     3001 ctccgccatt ttctattttg atttgctatg cgattaatct ttatcgtcaa atgtcaaaat
     3061 cgggatgccg gattgggtaa acagggcata caccggcttg agccaataat cttccggaag
     3121 ggaagccggt ttcaatgcgt aaacgtgcac attgggaaaa tccttcatcg tcaacgccgc
     3181 gccgctgaaa aaggtataaa tttcatacct cgtatgcggg tttttcttaa tctcgcgcaa
     3241 aatatagtct tcgatgacat agggcgaatt taatgtggtt acgccggaaa gcccgtaggt
     3301 ttggcggggg tgcggtgcga catattgtat gttgaagttt tttgccgcct tttccgaaat
     3361 ttccttcatc tccttgtcgg gcgaacccaa aagtatccgc accgtgccgc ccgtttcgtc
     3421 ccccgccttc agttcggacg catcgaacag cggcaggtaa gtcatcttgc ggcggccgtc
     3481 gtccataatg tttttcaaac ccttgaatat cgtgtaatgc tcgtcggaag cattacgggt
     3541 tttggcgatg ctccaatctc cgatcatcat ccgggcaaaa ttccgcttga tcgtcccgtt
     3601 tacagaaaac tcatcgccca aatagctgct gctttgaatt aaattgcctg tcccgtcgtc
     3661 aaaggttttg atttccgcat ccgggtaagt gctcaaaaag gcggcaatgc tgactttttc
     3721 caaacttgcc aaataaatcc gcttgacttt cggcagcagc atcgacttta ccttcagctc
     3781 cgccatcgtc ggaatgaaat taaacgattt gttcaaaccg tagggcaggt ggaaaaaata
     3841 cgcccgctcc gccttatcct ttatctgctt gaaataataa tcgtattttt cattcctgtt
     3901 ttcagacatc agcaccacat aaaaccgctc ccccggatgc tgcgccataa tcctttccgc
     3961 caccttcatc tgcaatatgg tatagcagaa aatcagattg accggttccc cctcttcatt
     4021 gaagagtttg tccttcagca gggaaaccgc attcctttcc ccatgattta cccggtcaaa
     4081 tgtataaaat atcccgaaac aaaaaacaat caaacacaac acggtcaaac aagccttttt
     4141 caagcccata tccctaaaac tccattccga caaattgaac atacgccccg agtgtcccgg
     4201 gacggaaaaa tgcggaattt tagcagatat tccgacagtt accgaatatt atagtggttt
     4261 aaatttaaat caggacaagg cgacgaagcc gcagacagta caagcagtac gacaaggcaa
     4321 ggcaacgctg tactggttta aatttaaacc actataaaat caatttcacc gaaaatatgc
     4381 cgccgcacat caggcacagc acctccgacc gccaccaaac gcgggaaaac catttccccc
     4441 ttcctttcac gctgccgcgt tcaaacacgc caagatcgaa caaagcgcga acaaagccgc
     4501 caaaatatgc cataatagcc gcgcacattc cccgcgcaat gtgttccaaa agaaaaaacc
     4561 aacctacaag agaataatcc tatgactcaa aaatccacca ttgtttatac ccataccgac
     4621 gaagcccccg cgctggcgac ccaatcgctg ctgccgattg tgcaggcttt tgcccgccac
     4681 gccgatattg atgtcaaaac tgccgacatt tccctgtccg gccgtatttt ggcggcattc
     4741 cccgaatatc tgaccgaagc gcaacgcgta cccgacgcgc ttgccgaatt gggcgaactg
     4801 gtaaaacaac ccgatgcaaa cgtaatcaaa ctgccgaaca tcagcgcatc cgtaccgcaa
     4861 ctgaccgccg cgattaaaga attgcagtct aaaggctttg ccgttcccga ttatcctgcc
     4921 gacccgcaaa ccgatgaaga aaaagccgtg cgcgaacgct acgaccgcat caaaggcagc
     4981 gcggtaaacc ctgtcctgcg tgaaggcaac tccgaccgtc gcgcccccaa agcggtgaaa
     5041 aactttgcca aaaaaaatcc gcacagcatg ggcgcatgga caaaagactc caaaacccac
     5101 gttgccacca tgcaaagcgg cgactttttc cataacgaac aatccgttac cgtacctgaa
     5161 gcgacttccg tatccatcgt gttcaccgac aaacaaggca ataaaaaaga gctgcgcgcg
     5221 cctgttgccc tgaaagccgg cgaaatcatc gacgcaaccg tgatgagcaa aaaagccctg
     5281 ctcgccttcc ttgccgaaca agtgaaagac gcgaaggcaa aaggcgtatt gttctcactg
     5341 catatgaaag ccacgatgat gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa
     5401 gtgttcttcg cgcctgtatt tgaaaaattc ggcggcaaac tggctgccgc aggtgtcaac
     5461 gtcaacaacg gtttcggcaa cctgcttgcc aatctggaca aactggatgc ggacacccgc
     5521 accgccgtcg aagccgaaat cgccgccgtt tacgccgcca accccgattt ggcgatggtc
     5581 gattccgata aaggcattac caacctgcac gttcccagcg atgtcatcgt tgatgcttct
     5641 atgcctgcga tgattcgtaa ctccggccgt atgtgggata aagacggcaa agcgcaagac
     5701 accaaagccg tgattcccga cagcagctac gccggcgttt accaagcaac catcgacttc
     5761 tgccgcgaac acggcgcatt tgacccgaca accatgggta ctgtgcccaa cgtcggactg
     5821 atggcgcaag cggcggaaga atacggctcg cacaacaaaa cctttgaaat cgaagccgac
     5881 ggccaagtcc aagtcattga tgcggcagga aaagtcctaa tgcagcacga cgttgaagcc
     5941 ggcgacatct ggcgtatgtg ccaaaccaaa gacgctccgg ttaaagactg ggtacaactt
     6001 gccgtcaacc gcgcccgtct gagcaacacg cctgccgtgt tctggctcga cgaaaaccgt
     6061 ccgcacgaca agagcctgct cgccaaggtt aaagcctacc ttgccgaact ggataccaat
     6121 ggcctcgaca tccgcgtcct cgctcctgaa gaagccgcca agttcagctt gggtcgtctg
     6181 aaaaacggcg aagacaccat ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac
     6241 ttgttcccaa ttttggaact cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg
     6301 aacggcggcg gtatgtttga aaccggcgcg ggcggttctg caccgaaaca tgttcaacaa
     6361 ttcctcgaag aaaaccattt gcgctgggac tcgctgggcg aattcctcgc actcgccgta
     6421 tcgtttgaac atttggcgca aaaaaccggc aatgccaaag cccaagtcct cgccgacact
     6481 ttggatgcag ccaccgaaaa actgctgttg aacgacaaat cgcctaaacg taaagccggc
     6541 gaactcgaca accgcggcag ccatttctac ctcaccctct actgggcgca agaattggcg
     6601 gcgcaagaca aagatgccga actgaaagcc gcatttacgc cattggcagc cgctttgacc
     6661 gccgacgaag cgaaaatcgt tgccgagctt tccgccgtac aaggcaaagc ggccgacatc
     6721 ggcggctact acgccgccaa tcctgaaaaa gccgcacaag tgatgcgtcc gagcgtaacc
     6781 tttaatcagg cactaagcac cttataagca caaaagtaaa aatgccgtct gaacatttat
     6841 cgttcagacg gcattagtta tccctatctg cctgattatg attaaacgcc gcacccattg
     6901 gatat
//
LOCUS       DAWFIT010000105         6857 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFIT010000105 DAWFIT010000000
VERSION     DAWFIT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.105"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..617)
                     /locus_tag="WGQ85_001914"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="WGQ85_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
     gene            complement(1968..2983)
                     /locus_tag="WGQ85_001915"
                     /pseudo
     CDS             complement(1968..2983)
                     /locus_tag="WGQ85_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            4257..6389
                     /locus_tag="WGQ85_001916"
     CDS             4257..6389
                     /locus_tag="WGQ85_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0751273.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            6613..>6857
                     /locus_tag="WGQ85_001917"
     CDS             6613..>6857
                     /locus_tag="WGQ85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0751274.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSL"
BASE COUNT         1857 a         1505 c         1436 g         2059 t
ORIGIN      
        1 ccaatgcccg caagattggc ggcttttgcc tgttcggcag tgagttccac tttgtctttg
       61 aagcggctgt gcagattgtc ggctgctaaa agtaaatccc ccatttttgc ggttgtaccg
      121 tctgttttgg tgtagctgcc ttgctgagcc aaagtgttac cgttgccgag atttttattt
      181 acatctttat aggcgagatc caaagattgg atacccaatt cttcaagggt acgcaattca
      241 ttagcttggg aaatgccgtc ctggttgaga tcctgccata cacgcaggga ttggaatgcg
      301 gcgtctgccg cgttgatgat gttgtcgccg tttgaatcca attcggccaa agccgcgtag
      361 ccgtgtttgg caaaagagcc gtctgccagt ttggtgttgt cgccgaagag ttccgcaccg
      421 ttgtcgatga tgccgttgcc gttcaaatcg cgcacaagca gaccgtcatc ggcagcaacc
      481 caacctgtgg cggtgcggat gccttgattg cggtggtcga acaatgcacc tgcaaagcct
      541 ttggcagcga cagtttctat gccgtcgccg tccaaatcta gggcaagggg gtcgtaaacg
      601 tggtattggc cgtttcgatt aagaaatagg aaatctcttg ctcctttata cccttcccat
      661 gctaaaccac ccaacccatc ttcaatccac ccccctaatt tgtctcgtaa ccactcagga
      721 gttaaaacac cagttccacc acccaataat ccgcctaaga ccgcgccacc aagagcaaac
      781 gcacctacag tcagtgcagc aggcaatccc gctaaaccaa gaccagtaac aataaaacca
      841 gcaacccatg caccaaatgc tcctcctatc tcacccaatg ctagtcctgt acctattttt
      901 gatgaagttt ttccaagatt atcatagtca ttttcattga ttgcttttgt ccatgcagaa
      961 ccgagttgaa ttcctgagat tgcacctgtt gcaaatgaag caaatttcgc agcagaggcg
     1021 gcttttgcag aatatatatt tgctgcatta ttagcggctc tgacggactg actaatttta
     1081 gataaatcat gtttcgtaac attgacttct cgagaaaatt ggctttgcat ttgccttaac
     1141 aaagatattt ctgctactcg tgctttatta tttatattat atttactctg catttttctt
     1201 acaacttgtc tcaactgact ttgtgttttt tgtaattcac gttgtaccga ttttcctaaa
     1261 ctttcatgtt tggctgcata attggtagaa tcagttgaca tttgtgtata aaaattttta
     1321 cgaaaatttt cgtaactcag attaattaca tccgtacttg cagttataaa atcttgtata
     1381 aattcaatag aaactgaatt atcatcagat ttggctttgt tattttccat attgagcctc
     1441 tcttaatttg aaaaaattaa cataaacatt taaaaataaa acaaaattat ataataaaaa
     1501 aaataatgag aatataaagg ataaaaagag agttaagtta tttaatcgga cttgttctct
     1561 ataaatcata aggcaccaaa taagccataa taaatttagt aaataaaaac tgcttatacc
     1621 caactttctt ttcttggaac ctattattat ttcaactctg gatgaaaaaa tagaatttaa
     1681 aaattttttt ctttgatata tcgttaatat ctccctagtt tttcttaaaa aaaatataat
     1741 catatatata ctacacaaca ttggtaataa ataaattaaa agcgaaaggt aattaaattg
     1801 ataattgtaa attatataat aaacaacata cgaagaaaaa attattatag atgatttgct
     1861 atacgaataa aaaaatatcc tacctagccc gaaccttctt ataatttgta ctatgtgatc
     1921 attcatttca atatccattt aagctttcaa ttaattttct atatttttca atggcatgta
     1981 tcatttggtt gatgatcaag ccatgtatgc aaagattgga atatggtatc tgtcgtgcta
     2041 ataatgcccc cgttactgtt taatttgcgc acaggaaaac cgtcatatgc agcaatccag
     2101 cccgtggcgg tgcggatacc ttggctgcgg tggtcgaaca atgcacctgc aaggcctttg
     2161 gtggcaacgg tttctatact gtcgccatct agatctaggg caagggggtc ggttacgtgg
     2221 aatttgtcgc tgcggttaag atttaagaaa tcttcaaacc atctccaagc tttttcagct
     2281 cctaatgatc cccaccaagc tcctgcgcca cctccaatta tagttccaac tactagccaa
     2341 cctgcgccac cagtaagtaa ataagtaaac caaaagcccc aacacccaaa gccgcacccc
     2401 attggaagcc cattagccca cccaaaaaag aagctgatga gagaccaaca tcactccaat
     2461 ttccttcttc atccaaggct ttccaccaag cacttcccat actcaaagtt cccatggcaa
     2521 cagttgcccc ataagtaaac ttacccaaat tcgctgccat aacttttgat tttgaagcat
     2581 tctctaacgc tgttgccgca cgtcttgcat attgctgctt gttatagttg ctagcattca
     2641 atgctttttg caaatcactt tgtacttttc ttgcaacaaa tgcctccttt gaattaccac
     2701 gaaatttact agcagctctt ccaattcctt cttttaagct tttttgtgcc tcttctgctg
     2761 tgcgttggaa ttctcttgcc ttagcagaca gacgctcacc ctcccttaaa aatgctcgtt
     2821 gatctatctt gtgcgaaagg gtcaaatatt cttcgtacat tgagttaatc gtaccaatcg
     2881 ccctaaccac attttcatca gaaaatatgg aaataatagc atccctatac gcacctagcg
     2941 taatattgtt tctattatta gttatagcat tattcgaata cataatagca cctccaaact
     3001 tagaactgaa tacgtttata ttctctgtgt aataatatca aataattaat taatggataa
     3061 gtaagtccga aaaaataaca actaaaataa gcaatatact gattctctcc tttattaatt
     3121 agatatattg aaaaacaaat aaaaaataaa cagaaaacat tcatgtgaaa aagccacctc
     3181 atattcttat ttataagttt aaaataacta ggcattgtat atttaaaaat cggtacatcc
     3241 aacggaaaca aaccacataa aaatttttct ttattcttta ttaaagacat agttttattt
     3301 ttcaaaatta gtccagataa tatactaaca attactatta aaacaataag cacagcgtaa
     3361 ttttttataa aattcattaa aataataacg aaaatactaa atagattaca tactgtaaat
     3421 tgaaatataa aatattcagt atttaatcca ttttcaaccg cgagtttttt aaaatatcct
     3481 tgctcgaaaa aattaaaaaa atccattaat actttcctct caagaaaact aattttagtt
     3541 tagttaagat aaaatgcaat attacctttt ctccataaaa gtatggagct tatatacata
     3601 aattatgtgc gaggtagcct gaaaaatatc caatacaaga tagtaggaga tgtttccctt
     3661 atcactattg gccaataccc aattggcagg cactctattt ccaaaatttt ctcaagaata
     3721 gttaaatatt attaattagt ttaaaaacaa taacatacgt ttgtatttat ccgttttggt
     3781 ctgcctgaaa cagactctat gtccatacac ggacatgtag acagtttcct tgttattttg
     3841 gttgctcgtc attagtgatg atactttact cctctcttaa aaaatcgtca agaactgtta
     3901 tcaatcttta aagtcttttt taaacaaata tatatacgtt caggctgtct gaaacacaaa
     3961 aaaatgatgt tacccgaccg tgacagagtc ggggcagccg gtaccttaac cctgcacctt
     4021 aaccctgtgc cttaacggta tataggaggc acgataggtg cgaggcatcc gcatctatct
     4081 tgattcctaa gccgttttac cttcctgaaa cgagaacttt tccatacttt ctctttacaa
     4141 ttcccatata tatccttaca attcgttctg ctgttttatt tatatatttc tttctactta
     4201 atatattcct tttccagcca gccgttatcc acatccaatc taactatatt ttatttatgt
     4261 ctatcgtatc cgcaccgctc cccgcccttt ccgccctcat catcctcgcc cattaccacg
     4321 gcattgccgc caatcctgct gatatacaac atgaattttg tgcttccgca caaagcgatt
     4381 taaatgaaac acaatggctt ttggccgcca aatctttggg attgaaggca aaggtagtcc
     4441 gccagcctgt caaacgtttg gctatggcga ccttacctgc attggtatgg tgtgatgacg
     4501 gcaaccattt tattttggct aaaacagacg gtgggggtga gcatgcccaa tatctaatac
     4561 aggatttaac tacgaataag tctgcggtat tgtcttttgc cgaattttct aacagatatt
     4621 cgggcaaact gatattggtt gcttcccgcg cttcggtatt gggcagtttg gcaaagtttg
     4681 actttacctg gtttattccg gcggtaatca aataccgccg gttgtttttt gaagtattgg
     4741 tggtgtcggt ggtgttgcag ctgtttgcgc tgattacgcc tctgtttttc caagtggtga
     4801 tggacaaggt gctggtacat cggggattct ctactttgga tgtggtgtcg gtggctttgt
     4861 tggtggtgtc gctgtttgag attgtgttgg gcggtttgcg gacgtatctg tttgcacata
     4921 cgacttcacg tattgatgtg gaattgggcg cgcgtttgtt ccggcatctg ctttccctgc
     4981 ctttatccta tttcgagcac agacgagtgg gtgatacggt ggctcgggtg cgggaattgg
     5041 agcagattcg caatttcttg accggtcagg cgctgacttc ggtgttggat ttggcgtttt
     5101 cgtttatctt tctggcggtg atgtggtatt acagctccac tctgacttgg gtggtattgg
     5161 cttcgttgcc tgcctatgcg ttttggtcgg catttatcag tccgatactg cggacgcgtc
     5221 tgaacgataa gttcgcgcgc aatgcagaca accagtcgtt tttagtagaa agcatcactg
     5281 cggtgggtac ggtaaaggcg atggcggtgg agccgcagat gacgcagcgt tgggacaatc
     5341 agttggcggc ttatgtggct tcgggatttc gggtaacgaa gttggcggtg gtcggccagc
     5401 agggggtgca gctgattcag aagctggtga cggtggcgac gttgtggatt ggcgcacggc
     5461 tggtaattga gggcaagctg acggtggggc agctgattgc gtttaatatg ctctcgggac
     5521 aggtggcggc gcctgttatc cgtttggcgc agttgtggca ggatttccag caggtgggga
     5581 tttcggtggc gcgtttgggg gatattctga atgcgccgac cgagaatgcg tcttcgcatt
     5641 tggctttgcc cgatatccgg ggggagatta cgttcgaaca tgtcgatttc cgctataagg
     5701 cggacggcag gctgattttg caggatttga acctgcggat tcgggcgggg gaagtgctgg
     5761 ggattgtggg acgttcgggg tcgggcaaat ccacactcac caaattggtg cagcgtctgt
     5821 atgtaccgga gcagggacgg gtgttggtgg acggcaacga tttggctttg gccgctcctg
     5881 cctggctgcg gcggcaggtc ggcgtggtct tgcaggagaa tgtgctgctc aaccgcagca
     5941 tacgcgacaa tatcgcgctg acggatacgg gtatgccgct ggaacgcatt atcgaagcag
     6001 ccaaactggc gggcgcacac gagtttatta tggagctgcc ggaaggctac ggcaccgtgg
     6061 tgggcgaaca aggggccggc ttgtcgggcg gacagcggca gcgtattgcg attgcccgcg
     6121 cgttaatcac caatccgcgc attctgattt ttgatgaagc caccagcgcg ctggattatg
     6181 aaagtgaacg agcgattatg cagaacatgc aggccatttg cgccaaccgg acggtgctga
     6241 ttatcgccca ccgtctgtcc actgttaaaa cggcacaccg gatcattgcc atggataaag
     6301 gcaggattgt ggaagcggga acacagcagg aattgctggc gaagccgaac ggatattacc
     6361 gctatctgta tgatttacag aacgggtaga aggcggggga tgggcgtgta ggctgctttt
     6421 ttcgtaacgt tctgcgtttt cagaccgtcg tttactgggt ttactgactg aaatgaaacc
     6481 aaagtgcagg ctgccggaaa gactttttct gtctttttag gcagcctgca cttctttttc
     6541 actcatctag agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt
     6601 aggggatttt ccatgagcac cttctttcaa caaaccgcgc aagccatgat tgctaaacac
     6661 atcgaccgct tcccgctatt gaagttggac caggtgattg attggcagcc gatcgaacaa
     6721 tacctgaatc gtcaaagaac ccgttacctt agagaccacc gcggccgtcc tgcctatccc
     6781 ctgttgtcca tgttcaaagc cgtcctgctc ggacaatggc acagcctctc cgatcccgaa
     6841 ctcgaacaca gcctcat
//
LOCUS       DAWFIT010000106         6553 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFIT010000106 DAWFIT010000000
VERSION     DAWFIT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.106"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(146..1501)
                     /locus_tag="WGQ85_001918"
     CDS             complement(146..1501)
                     /locus_tag="WGQ85_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0751275.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1698..2519
                     /gene="nadE"
                     /locus_tag="WGQ85_001919"
     CDS             1698..2519
                     /gene="nadE"
                     /locus_tag="WGQ85_001919"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0751276.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2589..3062)
                     /locus_tag="WGQ85_001920"
     CDS             complement(2589..3062)
                     /locus_tag="WGQ85_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0751277.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3365..3697
                     /gene="trxA"
                     /locus_tag="WGQ85_001921"
     CDS             3365..3697
                     /gene="trxA"
                     /locus_tag="WGQ85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0751278.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWASWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3750..4697)
                     /locus_tag="WGQ85_001922"
     CDS             complement(3750..4697)
                     /locus_tag="WGQ85_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0751279.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            5025..6413
                     /locus_tag="WGQ85_001923"
     CDS             5025..6413
                     /locus_tag="WGQ85_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0751280.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1566 a         1765 c         1725 g         1497 t
ORIGIN      
        1 aacgtatcaa tcgtcattcc cgcgcaggcg ggaatacggt tttttgaatt tcagtcattt
       61 ctgataaacc ccggcggctt tccgcttccg gactcccgct tttgcgggaa tgacggcgga
      121 gggggagcgg tttttccgat tgggtttaaa tgcaatcgaa caaatcctgc tgcccttgtt
      181 ctttgcttac gcgcacgtcg gtttcgccgt cggcgaaggt aatgtgcagc ttctgcccct
      241 gcttcaaaac atcggcgttg cggatgactt gtccgcgtgt gtttttgacg acggaaaagc
      301 cgcgctccag aatgtgctgc ggcgaaacgg cttcgagcaa tgcggcttgg gcggtcaggc
      361 tttggcggcg gtgggtaagc agttggctga aggcggcggg caatgccgtc tgaaggcgga
      421 cgatgttgtt tttgcaaacg gaaacatcag gacggtaatg ttgcagggct tgggtttggc
      481 gttcaaaacg ggcggtgtgg gcacggaggt tttgcttcat cgagtaagac agcgtttgcg
      541 ccagcttgcc gattgaagcg cgctgctcat caagtttttg gcgcggatga cggatttgcc
      601 gcgcaagcca gtcgagtttt tggctggcat cgaaatagcg ttgttccaaa acggttttca
      661 gacggccttg cgcctgagcg agacgatgta gcgattcttg gcggttgggg ctgaccagct
      721 ccgccgcacc ggtcggcgtg ggcgcgcgca cgtcggcgac aaaatcggca agcgtaaaat
      781 cggtttcgtg tcccacgcca ctgacgacgg gaaccgtgca ggcttcgatt gcgcgcacga
      841 ccggttcttc gttaaacgcc cacaagtctt caatgctgcc gccgccgcga cagacaatca
      901 acacgtcgca ttcggcgcgt tgcgaggcgg ttttaatcgc ttgggcaatt tgtaactcgc
      961 tgcctgcgcc ttgaacaggt gtcggataaa cgataacggg aatttcgggc gcgcggcgtt
     1021 tcaaggtggt aacgacatcg cgcaatgccg ctgctgccag gctggtgacg atgccgatac
     1081 attgcggacg ggtaggcaac ggctttttgc gttccgccgc aaacgcgcct tccgcctgca
     1141 actgtgcctt caaccgctcg taggcttcgt aaagctgccc caaacctttg agccgcacct
     1201 cattcacggt aatttgaaat tcgccccgcg cctcgtaaat actgattttt cctgatactt
     1261 cgatatggtc gccttctttc aaaggcttcg ccaaacgcgc cgccgcacct ttgaacatcg
     1321 cgcaacgcac ctgcgcgcgg ctgtctttga gcgagaaata ataatgtccg ctggcggcac
     1381 gggtcaggtt ggacacttcg cccgcaatcc acaggccggc aaggtggttt tccaaaagac
     1441 ttttggcaaa tgcgttcaac tcagaaacgg acaacacgtc agaatgaaaa aaatcagaca
     1501 tagaatcaat caaataataa aaaatatgaa tatgttttga agcctaaggc ggtattagtc
     1561 cgcctaaatt gtcaacaata ttataacacg cgccatcttg ccgcccgcct tttcccgtat
     1621 gactttttta agcggggaat gggaaaaata ttcatcaacc cgccctcaat ctattcaaat
     1681 tacaccgccg acaagctatg atgcggatat tttcgacagg aggaaaaatg gatacgcagg
     1741 cagttatcac acatattgtc cgatggttgg acgaatacgc cgcccgggca aatgcaaaag
     1801 ggtttgtcgt gggcgtttcc ggcggcatcg attccgccgt cgtctccgca ctcgccgccc
     1861 gcaccggccg ccccacgctg cttctggata tgccgatacg ccaacacccc gaccagcttg
     1921 agcgggcaag gctgcacatc cgcaatctgc aacggcaata tgccaatgta agcgcacaaa
     1981 cggtcgatct gaccgacacc ttccagacct ttgaacaaac cgtcggcgtt catcagacgg
     2041 catttgacaa tcagccgctt tccctcgcca acgccagaag ccgcctgcgt atgctgaccc
     2101 tgtactacta cgggcagata cacggactac tggttacggg gacaggtaac aaaattgaag
     2161 atttcggtgt aggatttttt accaaatacg gcgacggcgg cgtggacatc agcccgattg
     2221 ccgacctgac caaaacccag gtttaccggc ttgccgaagc attgggcgtg gacgaggcga
     2281 ttcaaaaagc cccgccgacc gacggcctgt gggatacgga acgcaccgac gaagaacaga
     2341 tgggcgcaag ctatcccgaa ctggagtggg caatgggcgt gtacggcacg cgcaaacccg
     2401 aagattttga agggcggcag cgcgaagttc tggaaatcta tacgcggctg caccgcgcga
     2461 tgcagcacaa aatcaacccg attcccgtgt gccgcattcc gcccgaattg ttgggctgaa
     2521 acgtggaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcattgac atatccggct
     2581 cctatccctt aagaatcgag tacgcgggca aacaaaatat cgttttccaa atgaatgtgg
     2641 tcgttcaaat cctccaccat ttctttggcc aaagcgtaaa gccgcgtcca gcttccgcag
     2701 gctccttcgg gcgcttggaa attatcggtc agctctttga gccttgcgat ggcgcggtcg
     2761 tgttcttcat gttcgtgcat catcacgctg atgggcatcg ccgcaccccg accgacaccc
     2821 tgattaatca tcggaaacag catcctttcc tctttcatca tatgcatcag cagttcgttc
     2881 tgcatatagg caagcagctc ggcaatttcc gccggaaagg tatcggcatg aacttgggcc
     2941 actttttgcg ccagcggcac caattcttca aattgtgcac ggtgtacatt gtggtagcgt
     3001 tgcaggatat gatcgacggt tgcaccaaag ggagcagttt cccaaacgga aaaatcagtc
     3061 atggcagggt tccttttatt tatagggttt cgggtttggt tttgaacatt catactttaa
     3121 gaatctattt aaacggagca tacaccgccc gtgcgtttct gtacagcttc aaaaacattc
     3181 cttacatttt gataataaaa gtaattttca aaaataaaat actgtccgaa ccgttttttt
     3241 agaatttgca aaggcgattg gggcggtaca gaaaaactat tatcccgccc gcccacttga
     3301 aatttttatg cccaagccct atcctgcacg ctatcgtgcc aatcccaacc gaaaaggaaa
     3361 aataatgagc agcgaattga tcgtacacac cggcgatgcc gccttcgagc aagatgtttt
     3421 gaaatccgac ctccccgtcc tgctggactt ttgggcttcg tggtgcggcc cgtgcaaaat
     3481 gattgccccg attttggacg acattgccgc cgaatttgaa ggccgtctga aagtagtcaa
     3541 aatcaacatc gacgacaacg aagccacccc gtcccgtttc ggcgtgcgcg gcattccgac
     3601 cctgatggtg ttcaaaaacg gcgacgtcgt cgccaccaaa gtcggcgcat tggcaaaagg
     3661 tcagctgacc gcctttgtcg aagcctctat cgcctgataa agcgcaatcg aaaaagccgc
     3721 cggaagattc cggcggcttt ttcgcaccct taagatttgt ggcggatttc ccagcaccga
     3781 tggatttttt tgttgcggaa atcttcggga acggattgtt tggaaatgtc tttgacggcg
     3841 tattgttccg ataccaagtc gtctaagacg aagctgcgca ggttgttgga aaagtacaaa
     3901 atgccgtctg aagcgagcag cttcaccgcg ccgtcaatca gctttttgtg atcgcgctga
     3961 atgtcgagga tgtcggacat tttcttgctg ttggaaaaac tgggcggatc catcacaatg
     4021 aggtcgaact gcctgccttc cccatatgcc gtctgaagat attggaacac gtcggcgcgg
     4081 acgattttgt gccgttccgt atcgatgccg ttcaattcaa aattgcgtct cgcccaatcg
     4141 agataagtgt tggacaaatc gacagtttcg ctggatgccg cgccgccggt ggcggcatag
     4201 acagtgaagc tgccggtgta ggaaaacagg tttaaaaaac gtttgcccgc cgccgtttcg
     4261 ccgacttttt tgcgcgtgtt tcgatgatcc aaaaaaagcc ccgtatccaa atacttatca
     4321 aggttgaccc aaaacttgcg gccgttttcg gtgatgacga aatcgtcgcc cgccttgccg
     4381 gttttttcgt attgctgcaa acctttttgg cgttcgcggc gtttgaggcg gatttgttcg
     4441 ggcgcaaaac cggtaacgaa ggcgacggct tccaagactt cggcaagcca cgcttcgtat
     4501 tcttcggacc gcatcagcca gccggtatcg tattcctgaa ggtggatttg gtcgccgtaa
     4561 acatcgacag caaaggggaa ttgggggatg tcgcggtcgt aaatgcgcca ggcttcgatg
     4621 ccgttgcgtt tcgcccattt cataaggtgt ttgatgtttt tgcccaagcg gttggcaaac
     4681 ggtgtgatgt cggtcattgg tttcaggcgg aataaagtgg aaaacggcaa ttttactgta
     4741 attaacgccc gattgcttga ccgtttcggg caaaccctat accatccgtc gcttatcttg
     4801 tcatacgaag ccatcgcctt ccaacctaaa ccgcccttac gggcgcgttt cttctgttgc
     4861 tttgattttg caaagcgtat ctgtgcaggt tgccgtcgat gtaaaccaca agcaagccgc
     4921 ttgcgacaac cctgtaactt cacattcccc gtatcgttac ccttccctgc ttcaggccgt
     4981 ctgaaccttt cggacgcgag cgttgttgtc ttccaaggat agccatgtct attaaatttg
     5041 ccgatttgaa ccttgataaa aacattttgt ccgccgtcag cagcgagggt tacgaaagcc
     5101 cgacgccgat tcaggcgcaa gccattccgt ttgctttgga aggccgcgac atcatggctt
     5161 cggcgcaaac cggctccggc aaaaccgccg cctttctgtt accgactttg cagcgactga
     5221 ccaaacgcag cgaaaaaccg ggcaaaggcc cgcgcgcgtt ggtgttgacg ccgacccgcg
     5281 aattggcggc tcaagtcgag aaaaacgcgc tggcgtatgc caaaaatatg cgttggttcc
     5341 gtaccgtcag catcgtcggt ggtgcatcgt tcggctacca aacccgcgcc ctgagcaaac
     5401 cggtcgatct gattgtcgcc acgccgggcc gtctgatgga cttgatgcaa agcggcaaag
     5461 tcgatttcgc ccgcttggaa gtgctgattt tggacgaagc cgaccgtatg ttggatatgg
     5521 gctttatcga cgacatcgaa accatcgtcg aagccacgcc ggaagaccgt cagactttgt
     5581 tgttctccgc cacttgggac ggcgcggtcg gcaaactggc gcgcaaactg accaaagacc
     5641 ctgaaatcat cgaagtcgaa cgcgtggacg accaaggcaa aatcgaagaa caactgctgt
     5701 actgcgacga tatgcgccac aaaaaccgcc tgctcgatca tatcttgcgc gatgccgaca
     5761 tcgatcaatg cgtgattttc acgtccacca aagccatgac cgaagtcatc gcggatgaat
     5821 tgtacgaaaa aggtttcgcc gccaactgcc tgcacggcga tatgccgcaa ggctggcgca
     5881 accgcacgct gatggatttg cgcaaaggcc gctgcaaaat tttggttgcc accgacgttg
     5941 ccgcacgcgg tatcgacgta ccgaccatta cccacgttat caactacgac ctgccgaaac
     6001 aggcggaaga ctacgtccac cgcatcgggc gcaccggccg cgcaggccgc acgggtattg
     6061 cgattacgtt tgccgaagtg aacgaatacg tcaaagtcca caaaatcgaa aaatacatta
     6121 accgaaaact gcccgaactg accatcgaag gcatggaacc gacccgcaaa cgcaaatccg
     6181 caggcggcaa gccgaaaggc aaaggcggct ggggcgatcg taaatccggc ggttggcgcg
     6241 gcgatcataa accgagcaaa gaaggcttcg gcggcaaaac gcgcggcgaa ggtttcaaga
     6301 aagaaggctt taagagagac ggtttcaaaa aaaccggcga aggcttcaaa ggcaaacgca
     6361 aagccggcga ttcttttgca ggcaaaggcg aacgccgtta caaagaccgc taagccccaa
     6421 cctgccgcat aaaccaatgc cgtctgaaac cgatttcgag tttcagacgg catttttaca
     6481 atgtttcagc accgcccggc tttgataccc aaaggattaa tctgcaatat agtggattaa
     6541 caaaaaccag tac
//
LOCUS       DAWFIT010000107         6424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFIT010000107 DAWFIT010000000
VERSION     DAWFIT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.107"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..687
                     /gene="mafB"
                     /locus_tag="WGQ85_001924"
     CDS             <1..687
                     /gene="mafB"
                     /locus_tag="WGQ85_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0751281.1"
                     /translation="PAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALV
                     NVLPFAKVKNLTKAAKLGKAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIH
                     EKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEIS
                     DIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDR
                     TSNPKYNPVK"
     gene            702..932
                     /locus_tag="WGQ85_001925"
     CDS             702..932
                     /locus_tag="WGQ85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0751282.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1081..1755
                     /locus_tag="WGQ85_001926"
     CDS             1081..1755
                     /locus_tag="WGQ85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0751283.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNECPTKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDADTGNHVYVKGEKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            1733..2014
                     /locus_tag="WGQ85_001927"
     CDS             1733..2014
                     /locus_tag="WGQ85_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751284.1"
                     /translation="MENGHRNNRSPLEKRIFYLEHSGQYLMICALSDYSKNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            2070..2243
                     /locus_tag="WGQ85_001928"
                     /pseudo
     CDS             2070..2243
                     /locus_tag="WGQ85_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            2224..2463
                     /locus_tag="WGQ85_001929"
     CDS             2224..2463
                     /locus_tag="WGQ85_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751285.1"
                     /translation="MVDAVVKTPEFVPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLIAVKKKQNYFDILLSGAIFYGR"
     gene            2453..2680
                     /locus_tag="WGQ85_001930"
     CDS             2453..2680
                     /locus_tag="WGQ85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751286.1"
                     /translation="MGVNLIGLNFEDFKQLFHHPKSYGVDEYYLSNESYPTYVYEFDE
                     IGVQAWEAKGKVVTIIAGGKDNYSTEPYYDE"
     gene            2687..2743
                     /locus_tag="WGQ85_001931"
                     /pseudo
     CDS             2687..2743
                     /locus_tag="WGQ85_001931"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2749..3126
                     /locus_tag="WGQ85_001932"
     CDS             2749..3126
                     /locus_tag="WGQ85_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0751287.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            3431..3832
                     /locus_tag="WGQ85_001933"
     CDS             3431..3832
                     /locus_tag="WGQ85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751288.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            3998..4447
                     /locus_tag="WGQ85_001934"
     CDS             3998..4447
                     /locus_tag="WGQ85_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751289.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPKNNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
     gene            4621..5754
                     /locus_tag="WGQ85_001935"
     CDS             4621..5754
                     /locus_tag="WGQ85_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751290.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(5841..6117)
                     /locus_tag="WGQ85_001936"
                     /pseudo
     CDS             complement(5841..6117)
                     /locus_tag="WGQ85_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6134..>6424)
                     /locus_tag="WGQ85_001937"
     CDS             complement(6134..>6424)
                     /locus_tag="WGQ85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0751291.1"
                     /translation="EAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEEDN
                     YSTSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
BASE COUNT         2135 a         1174 c         1375 g         1740 t
ORIGIN      
        1 cccgccgagg gcaaattcgc cgccatcggc ggcttgggca gcgtggcggg ctttgaaaag
       61 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc
      121 gaagccctgg tcaacgtcct gccgtttgcc aaagtcaaaa acctgacaaa ggcggcaaaa
      181 ctggggaagg ctgcggttag cggggatttt tctgctgcat acaatacaag aacaactaga
      241 aaagttacta cagaaacaga ggggttaaat agaatcagac agaaccagaa aaatagtaat
      301 atacatgaga aaaattatgg aagagataat cctaatcata ttaatgtttt atctggaaat
      361 tctatacaac atatactgta tggagatgaa gcaggaggtg ggcatctttt tcctggcaaa
      421 cctggtaaga caacattccc ccaacattgg tcagccagta aaataactca tgaaattagt
      481 gatatcgtta catccccaaa aacgcaatgg tatgcacaga ctggaacagg cggcaaatat
      541 attgctaaag gaagaccagc taggtgggta tcatatgaaa cgagagatgg aattcgtatc
      601 agaacagttt atgaacctgc aacaggaaaa gtggtaactg cattccccga tagaacctct
      661 aatcccaaat ataaccctgt aaaataatga gtatttgaaa gatgaaaaca ttagacgaac
      721 gaataaaaaa tttaggaaaa tctcttgaag atagaattga cgctaatcta attgatgccg
      781 cactagagta cattactttt tcagaacgtt tgctagcttt tgaaacgcta tgtgattata
      841 tagaagattt caatatccag cttacggaaa aagaatccca agaaatctct tttattaata
      901 aggaatttgg aattgaaagt acatcagatt aataaatatt aggtcggttt aaaaacccaa
      961 caatcaaaaa gtcgtctgaa actgtgttta atttacagac gacctttcct tcatttgaag
     1021 caggatattg agaactgagt tcttcaaaaa tcctacacct gctccttcca cggcagcacc
     1081 ttggtcaaaa cggcagacgg ctacaaagcc attgcccata ttcaagccgg cgaccgcgtc
     1141 ttcgccaaag atgaaaccag tggaaaaact ggatacaaac ctgttaccgc ccaatacggc
     1201 aatccgtatc gggaaaccgt ttacattgaa atttcagacg gcatcggcaa caaccaaacc
     1261 ctgatttcca ataaaatcca cccgttttac agtcaaggaa aatggataca ggcaggtcgt
     1321 ctgaaaaaag gcgacaccct gctttccgaa agcggtgcaa aacagacggt tcaaaacatt
     1381 accctcaaac agcagccgct caaagcctac aacctgaccg ttgccgactg gcatacctat
     1441 ttcgtcaagg gtaatcaggc ggaaacggaa ggggtttggg ttcataatga gtgtccgacc
     1501 aaaccaaaac caactaatca tgcccaacaa agaaaagaag aagctaaaaa cgattctcat
     1561 cgaagtgtgg gagattccaa tcgtgtcgtt cgcgaaggaa agcaatattt agatgccgac
     1621 acaggaaacc atgtttatgt aaaaggagaa aaagtggtta ttctaactcc tgatggaaga
     1681 caggtaactc aatttaagaa ctcgaaagcc aatacgtcaa aaagggtaaa aaatggaaaa
     1741 tggacaccga aataatcgat ctcccttaga aaaaagaatt ttttatctgg agcattcggg
     1801 acagtatctg atgatttgcg ccctatcaga ttactccaaa aataaacata ctgtcgttat
     1861 ggcaaatttt ctctatccaa atgaaaaaat ggattggaga aatttagatg atttgttcaa
     1921 tgagttggtt ttagaggaat tacagtcttc atttatggat tggtatccaa ctattgaaga
     1981 ggcaattagc catcatttag aagatttttc gtaacaagca taaatcaaaa agtcgtctga
     2041 aactgctttt aattttcaga cgaccttgag atggcggtcg aagtcaaata tcagcggaat
     2101 caaaagttgc tgaattagaa aataaaatta ttcccggaat gcccaaaaat gaacgcttaa
     2161 agattaagca aaaaatcaga aatattacaa aaaatgccaa tcgaaaagca aaaggagaag
     2221 agcatggtcg acgcggtcgt taaaactccc gaatttgtac cttttacttc tgtaggaata
     2281 ttccgttttg gtgcggatat aacacaatac aaaaacatac tggaaacttt tatgtatgaa
     2341 cctcctgatg agtttagaac agaatattat gaatctcccg actcaaattt actcattgcc
     2401 gtcaaaaaaa aacaaaatta tttcgatatt ttgttatcag gagctatatt ttatgggcgt
     2461 taatttaata ggtctaaatt ttgaagattt taaacaactt ttccatcacc ctaaatcata
     2521 cggtgttgat gaatattatt tatcaaatga atcttatccg acatatgtct atgagtttga
     2581 tgaaataggg gtacaagcat gggaagcaaa agggaaagtt gttactatca ttgccggggg
     2641 taaagacaat tattcaacag aaccttacta cgatgaataa cattacagaa aagaaacaag
     2701 aaacggtaca tttgataaaa acctaaatcg tattggagat taattattat gtgtgagttc
     2761 aaggatttta gaagaaacat cccttgtttt gaagagtatg acgaaaattc atttattggc
     2821 aaatggtatg atgacggggt gtgggatgat gaagaatatt ggaaattgga gaatgattta
     2881 atcgaggtta gaaaaaaata tccttatccg atggatatac caagggatat tgtgattgga
     2941 atcggtacca ttattgattt tttaatggtt ccaaattggg agctttttga aattaaagct
     3001 tccccttggt tgcctgatag tgtgggaatt catgaacgtt atgaaagatt cacaacgatg
     3061 ctccgttata tttttaccga gaaagacata gtcaacgtgc gatttgatta ttacaacaaa
     3121 aaatagcaaa acccggcaat caaaatgccg tctgaagcga tatttgcttt tcagacggca
     3181 ttttgatgaa tgaaacagga tattaagaac taagttcttc aaaaatcctg cctccgtccc
     3241 ttatacgtcg gtgctttagt caaaacggca gacggctgaa aagcaaacac tgtcatcaac
     3301 catttgaaga ccggatggaa taggaaagaa aatcggtaaa atctccacga ttagacggta
     3361 aaatacctcg gtgtcgctga aaccctgatc gtcgtgttgc caaaacaaaa atcagatatg
     3421 gaaaacgata gtgaagatta aaccattaca attttctaac aatagtcgca gattttacgt
     3481 agacaaaatt tgtgttaccg aacaagatgt gaacattttg gcatccgacc gaaattatga
     3541 attaaatatt gaaatattta ttgacaatat aattcatttt caaataacag atgaatctta
     3601 taaagtaaaa ttttcagaat atttatttga aaataaagaa aaaaatgatt gggatagaaa
     3661 tcctgctata aattattttt tcgagataat agatgacagt tatatggact ggttgaaaga
     3721 agaaagtttt ggtttttttg aaaagaaata ttataaggct tacattttct tttttagcga
     3781 ttctgtaata gaagttatca gctcgacaga acctgtattt tattcaaaat aacaaattat
     3841 caaacaaagc tctgattaaa aacccaacaa tcaaaatgcc gtctgaagcg gtattcggct
     3901 ttcagacggc attttctccg attagcacag gatgccgggg cttgccggct tgattttgcc
     3961 gaaattgttg gcacgcaatt gggaggggcg gcaacctatg gaaaatagat ttgaaaaaat
     4021 cgttaaggat atttttagtt ttacccaatt tgtttccgaa gcagaaaatg agattcctaa
     4081 ccaatcctcc cgttattacg actgttggtg ggaattggaa atcttgaatg cttccgcatt
     4141 ggaagattgg gaaatatcgg gaaagcctga aaaatgggag aaatggacag aaaaatatca
     4201 gaccgaagcg ttacagtcag tcaagcatct atttgccgaa gaagttccta aaaataatat
     4261 agtttgcaat atttctcatt ttttgcattt tgccgatcgg gcagaagccg acattcccaa
     4321 tcaatcacgt tcatatgccg aatattggtg ggaaatggaa atgttcaaaa tgtcggtttt
     4381 agaagattgg gaacgctcgg gaagaccgtt aaattgggaa aaatggcaaa ggcaatatcg
     4441 atcttaagca tttgaattag ttgaaaattt gaatcaaagc aaacaagagt aacaagccgc
     4501 agcctgcata ggttttttta aagtacggca gcaagcgtag attagattaa aaattaaaca
     4561 atcaaagctc gtctgatata atcgatatat cctgttaaac gaattaaacg aaaaataaat
     4621 atgtttaaaa aattcaaact ggtattgttg tcattttttg cccttgtatt tgccttttgg
     4681 ctggggacgg gtattgccta tgagattaat ccgcgttggt ttttgagcga tacggcaact
     4741 gaaatacctg aaaatccgaa tgcttttgtg gcgaaacttg cccgcctgtt ccgaaatgcc
     4801 gacagggcgg ttgtcatcgt gaaggaatcg ataaggacgg aggaaaatct tgccgaaact
     4861 gtggatgacg gtccgttgca gtcggagaag gattatctcg cgctcgctat ccggctcagt
     4921 cgtttgaaag aaaaggcgaa atggtttcac gtaacggagc aggaacatgg gaaagaggtt
     4981 tggctggatt accatatcgg cgagggcggt ttggttgcgg tttcgctttc gcaacgctcg
     5041 ccggaagcgt ttgttaatgc cgaatatctg tatcggaacg atcgtccgtt ttctgtaaat
     5101 gtgtacggcg gaacggctca cggagaaaat tatgaaacga caggagaata tcgggttgtt
     5161 tggcaaccgg acggttcggt atttgatgcg tcggggcgcg ggaaaatcgg ggaagatgtt
     5221 tatgagcatt gcctcgggtg ttatcagatg gcccaggtat atttggcgaa ataccgggat
     5281 gtcgcgaatg atgagcagaa ggtttgggac ttccgcgaag agagcaaccg gattgcatcg
     5341 gactcgcgcg attctgtgtt ttatcagaat atgcgggaat tgatgccccg ggggatgaag
     5401 gcgaacagtc ttgtggtcgg ctatgatgcg gacggtctgc cgcaaaaagt ctattggagt
     5461 ttcgacaatg gaaaaaaacg ccagagtttc gaatattatt tgaaaaacgg aaatcttttt
     5521 attgcacaat cttcgacggt agcattgaaa gcggatggcg taacggcgga tatacagacc
     5581 tatcatgcgc aacagacgtg gtatttggat ggcgggcgga ttatccgcga agagaaacag
     5641 ggagacaaac tgcctgattt tcctttgaac ttggaaaatt tggaaaaaga ggtgagccgt
     5701 tatgcagagg ctgcggcgag acgttcgggc ggcaggcgcg acctttctca ctgaacgcaa
     5761 atgccgtctg aaaccggttt tcagacggca ttggagagtt tgacgctttt tgcaatgccg
     5821 tatattccaa atccaattcg ctacaaattg aaaaccacaa tccaaaaaac ggaaatacac
     5881 attaaacggg tctttttacc gaccgtatca tcccataggt tttccacccc tttttttcat
     5941 ccataaattc aaaccattta agtttgcatc cttaccgaca attgcagcag catcatccgc
     6001 ataacggtca cccaaattag ccaaataacg ccaaccgcca taggtatcgc cacggaaagc
     6061 ataaccacgc aaggtttgga tttgttctgg tgttaaaaca acttcaccct cattcataat
     6121 aagatccttt aaattatttt ttacttggat tgtttacaaa actctgtacc aattgctcta
     6181 cttgttggtg ataacctacc aactcctgct gtgtgagctg ctgcttttcc cgaataaacg
     6241 ggtaataccg taccgtgaaa ccgttaatac aggaacctac gctagtgcta taattatctt
     6301 cttcagtttg gtttattcga ttattccgat gagtaataat cgccggattt tctccttttt
     6361 taatatagta aagtttatga aaatatggat tttcaggaat atgtctttta tcttcataag
     6421 cttc
//
LOCUS       DAWFIT010000108         6259 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFIT010000108 DAWFIT010000000
VERSION     DAWFIT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.108"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(391..825)
                     /locus_tag="WGQ85_001938"
     CDS             complement(391..825)
                     /locus_tag="WGQ85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0751292.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGQ85_001939"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGQ85_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0751293.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGQ85_001940"
     CDS             complement(1556..2398)
                     /locus_tag="WGQ85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0751294.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGQ85_001941"
     CDS             2653..3291
                     /locus_tag="WGQ85_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0751295.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WGQ85_001942"
     CDS             complement(3743..4543)
                     /locus_tag="WGQ85_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0751296.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WGQ85_001943"
     CDS             complement(4679..5182)
                     /locus_tag="WGQ85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751297.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WGQ85_001944"
     CDS             complement(5519..6160)
                     /locus_tag="WGQ85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0751298.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1665 a         1565 c         1306 g         1723 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttatacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcgaaataa agtggcacgc ccgatttttc actcataccg attaaataat
     5581 ccatcgtctt ccgagggaac gcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacaa cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataag
//
LOCUS       DAWFIT010000109         6234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFIT010000109 DAWFIT010000000
VERSION     DAWFIT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.109"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..69)
                     /locus_tag="WGQ85_001945"
     CDS             complement(<1..69)
                     /locus_tag="WGQ85_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0751299.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
     gene            complement(211..2217)
                     /locus_tag="WGQ85_001946"
     CDS             complement(211..2217)
                     /locus_tag="WGQ85_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0751300.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2506..4533
                     /locus_tag="WGQ85_001947"
     CDS             2506..4533
                     /locus_tag="WGQ85_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0751301.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4625..6178)
                     /locus_tag="WGQ85_001948"
     CDS             complement(4625..6178)
                     /locus_tag="WGQ85_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0751302.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1282 a         1456 c         1887 g         1609 t
ORIGIN      
        1 tggagcagtt ctttctgtac ttttttcttt aatttgcagt gcgttatctt catatttcga
       61 gggtaacata tctgctaatc tagtacagcc ccttttaaaa tgccgtctga accacgtttc
      121 ggttcggacg gcatttttgc atccttttat ggggatttcc cgcatcaggg cggtattcgg
      181 ggcgaatccc gcttcagacg gcattgccgg tcaaggcttg tccgaaggag gatgtccctg
      241 ttctttggcg ggcgggagga aaagtatgca gggattgctt ttaatctgat tccaaaaact
      301 cttaatcaga ttgcggatgc tgccgatttt cgtgccgcgc gagcgcaggg cgacgaagag
      361 ggcgaggctg aagctgacgc agaggttgac caggccgatg gcgaggacgc tgaaaatgcc
      421 gagtacgaac gtgcccaaac cgacgttgcc gctgacggcg gcatagccga ggttggcgga
      481 ggaaaaggcg acgtggcgga tgtccagcgg cagcccgagg aggtgtccga aatagccggt
      541 catacccaag agcatcccga agatgaagtt gcctgccaac gagccgtagt gtttgtggat
      601 gtaggcggcg aggacgcggc ggggcccggg gcgcatgatt ttacgcagca aggggttgaa
      661 gggcaggcgt tggcgcaggt tgaggtagtc ggcgcggttg tcgaaaaaac ctgcgatgat
      721 gccggagcag aacagccaca gacctgcaat ggcggcatac cacagcgtcg ggtaagcgat
      781 gatgtctatg gatttgaact ggtaggcggc ggtgtgggca tcgagtatgg gctgccggta
      841 cagatgggca tagccgaacg atatggcgca cgccaaaagg atggcgatgg aaacgttgcc
      901 gaaaacggcg acactttggg aacggcatac gtcgatgagg agcttggcga gtttgttgtc
      961 caccgctttg ccgccttcgt tgagatcgac ctgttcggca aagctggcgg cagtcatcgc
     1021 gggctgtttg gtggcgacgg tgcagtgcag catatggatg atcataaagc cgatgccgta
     1081 gttgaaccca gccgacaagg aagtcaggaa ggggctgagg ccgagtgtgc cgatgcggat
     1141 tttgagcagc gccatcaggg cgatgatgat gccgccgcct gccgccgagt agaacatttt
     1201 gaaatattct ttgcggttgc gggtaatgta gtgttcgccg tggtggcttt tgttttcggt
     1261 aatgctgcgg gcgagcatac ggatgctgct gcggcgcagg tggcgggtgc tgtattgttc
     1321 gaccgccgcg tagatgaggg agttcatcag cgcgatggtc aggcggttgc tgttgccggc
     1381 gccggtttgg atgtcggtga gcagcttcag acggtctacg gtctgccgga gccgttcgag
     1441 caggtgggcg actttgaccg acgaaccgga gccggcgccc gtccctttgc ggcgcaaata
     1501 gtcaatctgg ctgaaacatt ggtcgaacat cacttcgagg tgggcggtgt cgtaaggcgt
     1561 ggtgccgttg cggtagtgtt cgaccagttt ggcggtttcg cgttggaggg cgacgaagga
     1621 agaatcggct tccagcaggc gcggggcgat gcggatgagg tcgggttcga tggcttcgga
     1681 tgccgtccag acagacagca tttcgatggc gcgcaaacgc gcatcggcaa ggcggatgcc
     1741 cgccgtctgc aatttggacg gctcggcgtg gctccggatg agttcgtata aaaccagcca
     1801 ttggcggata ttgagcgttt gcagccattt gtcgtcgttt tcggaatgga aaagataaag
     1861 gaagacttcg cccaagttgg caaaatcttt atatgacggg ctgaagcgtt cgtagatgcg
     1921 tatgcccatt tcccgggcaa agctgtggcg cgagaagatg ccgagtttga tgagtgccgg
     1981 ataaatgtgt atttgcgcga gccagatgta aaaacaccgg ctgaagcggg acagcaattc
     2041 cctgtcttgt ttgaatgtgt cgataatcag gtcgaaacgt tcccccgcac attttttgcc
     2101 gccacggcgc aaaaatttaa tcagtgcgtt gaggacgttg acaaagtcgg tatgtcccaa
     2161 gctttccgaa agcagggggc gcaggttttg aggggtaatt ttcttcatat cagccattgt
     2221 accgcatcgg caatccggcg ttaaacgccc cgatggcggg gagggcgcgc ctgcggaagg
     2281 cggcgcattt tccgccggtt tgaaatcaaa ctatattaat gatataataa atatcgataa
     2341 atcaatctga tagaataatc cgtattgatt tttaattttt tcgattataa caatttcggc
     2401 cggtttgtgc atttcgtcat ccggaagccg gtattttgcc ggcaggtttt gaccgctttg
     2461 cccgatgacc gggtttgtct gtttttaaca aaggagtgag aaggtttgtc cctgtctgaa
     2521 tttatagaac gccgaacgtc atttaatctg atggttattt tgacgacttt gttttttgtg
     2581 tgtgttttgg tggtattggt tttaaccgtg ccggatcagg tgcagatgtg gctcgatcgg
     2641 gcaaaagaaa tcatttttac cgagttcagc tggttttatg ttttaacgtt ttccattttt
     2701 ctgggtttcc tgctgatact ctcggtcagc agtttgggaa acatcaggct cggacgggat
     2761 gaagatgtgc cggaattcgg cttcctgtcg tggctggcga tgctgtttgc ggccgggatg
     2821 ggcgtgggtc tgatgttttt cggcgtggca gagccgttga tgcattattt ttcggacatt
     2881 acggccggca cgccggaaca caggcagcag caggcattgc tgcacacggt gttccattgg
     2941 ggcgttcacg cttggtcggt gtacggtacg attgcattgg ctttggctta tttcggtttc
     3001 cgctacaagc tgccgcttgc cctgcgttct tgtttttacc ccctgttgaa agaaaaaatt
     3061 tccggaaggt tcggcgatgc cattgatatt atggcgttgc ttgctacttt tttcggcatc
     3121 atcaccacat tggggttcgg ggcttcgcaa ctgggcgccg gattgcagga aatgggctgg
     3181 attgccgaaa acagcttcag cgtgcaggtt ttgattatcg ccgccgtcat gtccctcgcc
     3241 gtcgtttcgg caatatccgg cgtggggaag ggcgtgaagg tgttgagcga gttgaacctg
     3301 agccttgcgt ttttgctgct gttttttgtt ttggcggcgg gtcccactgt ttacctgttg
     3361 tcggcattcg gcgacaacat agggaactac ctcggaaatc tggtgcgcct cagttttaaa
     3421 acttatgcgt acgaacggga acacaagccg tggtttgaat cttggacggt gctttattgg
     3481 gcgtggtggt gttcttgggc gccgtttgtg ggtttgttta tcgcgcgcat ttcaaagggg
     3541 cgcaccatcc gcgagtttgt cttcggggtt ttgctcatcc ccggcctgtt cggcgttttg
     3601 tggtttaccg tcttcggcaa tacggcgatt tggctgaatg acggggttgc ggggggaatg
     3661 ctcgaaaaga tgacctcctc tccggaaacg ctgcttttta aattctttaa ttacctcccc
     3721 ctgcccgaat tgacgagcat cgtcagcctg ctggtcattt ctctgttttt tgtaacttct
     3781 gccgattccg ggatttatgt cctgaacaat attacctctc gggacaaagg cttgagcgcg
     3841 ccacggtggc aggcggttat gtggggcgtg ctgatgtctg ccgttgccgt tttgctgatg
     3901 cgctcgggcg gactcggcaa cctgcagtct atgaccctga ttgtttccct gccgtttgcc
     3961 ctgctgatgc tgataatgtg tttcagcctg tggaaaggct tgagtgcgga taagaaatat
     4021 tttgagaccc gggttaaccc taccagtgta ttttggacgg gcggcaagtg gaaagaacgg
     4081 ctggtgcaga taatgagcca gacgcaggag caggatattt taaaattcct caaacatacc
     4141 gcatcgcccg ctatgcacga gttgcaacgg gagctttcgg aagaatacgg cttgagcgtc
     4201 cgggtcgata agatgtttca tcaggacgag cccgcaatcg agttcgtcat tcggaaagag
     4261 acgatgcgcg attttatgta cgggattaag tctgtcgggc aggatgtatc cgaccagttg
     4321 attaacgacg gcaagctgcc gcatatccgg catcagacaa cttacaaacc ctacgcttat
     4381 tttttcgacg ggcgcgtcgg gtacgatgtg cagtatatga acaaggacga gctgattgcc
     4441 gacattttga aaaactacga acgttatttg atgttgttgg atgatgtcgg tcaggaactg
     4501 atggcgcacg agcaggtgga attggcagag taagtgcctt atctctggcg tttgccaaaa
     4561 aaaatgcggt gaatcagatt caccgcattt tttttgccgg acggggcttc ggacggcacg
     4621 gcgttcattt gtttccgtca aactgctcct tcaacacccg tttcaatacc ttgcccgtag
     4681 cgttgcgcgg cagcccgtct ttaaagtgga tttgtttggg gattttgaaa tttgccagca
     4741 cggtacgcag gtggcggcgg atttcgttct cgcccaaatc cataccttcc ttcaattgga
     4801 cgaaggcgac gatttcctcg tcggcataac ggtctttcac gccgatgacg gcggcggctt
     4861 cgacggcatc gagtttgtag atttcttctt cgatttcgcg cggatagacg ttttgacctt
     4921 tggaaataat caaatctttt ttgcggtcga cgataaagat aaagccgtct tcgtctatgg
     4981 taacgaaatc gcccgttttc aaccagccgt tgacgatggt ttcatcggtg gcggcaggca
     5041 tattgaggta gccccgcatc accgaaccgc ccctgacgat cagttcgccc acttcgccgc
     5101 gcggcacttc gaccaattct tcatcgacgg ctttggcttc caaaccgggc agggggatgc
     5161 cgacgctgcg ggctttttgc ctctcgggcg tattgacggc gacgacgggc gaggcttcgc
     5221 tcagtccgta gccttccagc aatttggcgc gggggaactt ggctttgaaa tcgaggatgg
     5281 tttgttccgc caaaggtgct ccgccgctga taaacaggcg gatgcggttg aaccatctga
     5341 aataccaagg gattttcgtc ttgctcatcg cggtgtaaat cgcgggcacg cccaaaaaca
     5401 cggtcgcgcg tttgagcagt gcctgtttca aaacgttgga aaaggggaaa acggatttga
     5461 ccaaaataat cgaacacgcc atataaatcg gcagcagcac catagccgtc agcgtgaagc
     5521 tgtggaacat cggcaggaaa acgataaagc ggtcgcgctt ggagatttta aagatgcgtt
     5581 cgatgccgtt caggttggcg aacaggttgg cgtagctgat tagcgcacct ttgggatgcc
     5641 ccgtcgtgcc ggaggtgtag atgatgtgtg ccaaatcatt tatccggggt tggcggccca
     5701 agtcgggttt ttcggggaag cggcgcacgt cttcaaaaaa ggcatcgcct tccgccattt
     5761 cgccgtccgg acggctttgg cccgtccaaa tgattttttc gacgggcgtt tgcgccttca
     5821 agcccgccaa ttcttttgac aggccggccg aggcgaacag gaagcgcgcc ttgcagtcgt
     5881 tcaggatata cgcgtattcg ctgtttttca aaaatgtgtt catcggtacg gcaaccgcgc
     5941 cgatggcgga tacggcgaaa taggcggtaa taaattccgt ggaattggaa accgccagcg
     6001 cgaccgtgtc gccgaacttc acgccgatat tttgcagata cgccgcaacg gcttcggctt
     6061 cctgcttgag cgcgcggtag gcggtttttt ccttgccgtc gaacactgcc gtgccgttgc
     6121 cgtttttgcg gcaggcggcg gtcagcattt cgtagaaatt ggcataagtc cggttcatga
     6181 aacttccttt atggttgcgg acggcgaacg ccctcatttg aaaacaatat gccg
//
LOCUS       DAWFIT010000110         6207 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFIT010000110 DAWFIT010000000
VERSION     DAWFIT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.110"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            24..407
                     /locus_tag="WGQ85_001949"
     CDS             24..407
                     /locus_tag="WGQ85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0751303.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            522..995
                     /locus_tag="WGQ85_001950"
     CDS             522..995
                     /locus_tag="WGQ85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0751304.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMRIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1121..2224
                     /gene="mnmA"
                     /locus_tag="WGQ85_001951"
     CDS             1121..2224
                     /gene="mnmA"
                     /locus_tag="WGQ85_001951"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0751305.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2295..3965
                     /locus_tag="WGQ85_001952"
     CDS             2295..3965
                     /locus_tag="WGQ85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0751306.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWKRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4077..5711
                     /locus_tag="WGQ85_001953"
     CDS             4077..5711
                     /locus_tag="WGQ85_001953"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0751307.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5726..5928
                     /locus_tag="WGQ85_001954"
                     /pseudo
     CDS             5726..5928
                     /locus_tag="WGQ85_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5939..>6207
                     /locus_tag="WGQ85_001955"
     CDS             5939..>6207
                     /locus_tag="WGQ85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751308.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDPVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDSELEHSLITRIDFNL"
BASE COUNT         1622 a         1693 c         1551 g         1341 t
ORIGIN      
        1 gagaacattt tgaaaggata ccgatggaac cttcttctta cgcggcagaa aaaaaaggca
       61 aaggcggcat caggcgcgtc attaacgcat tcggctattc gatagacggc atcgccgccg
      121 cctaccgtta cgaagcggca ttccgtcagg ttttgtggct gaacgtactg ctggtgtgcg
      181 cggcattttt ttgggtttcc gaaaccgccg tccgcctgcc gttgattatc gcgtcttttg
      241 tgtcggtcat tgtcgaactg ttcaacaccg ccgtcgaagc tgccgtcgat catacttcga
      301 ccgaaaaaca cgagctggcc aaactcgcca aagacgcagg ttctgccgca caattgttcg
      361 cgatgctgat gttggcggcg gtttggctgt ctgccctgtt cgggtaaaac gcttgcagca
      421 ggatttgtaa tcctttagga ttagtatgtg gtatttatta ttcacttaat ccgctataag
      481 attaagcaca ttccccgtct gatattataa ggagcagaaa gatgaaaaaa ttattggcgg
      541 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgccgc cggagtccac gttgaggacg
      601 gctgggcgcg caccaccgtc gaaggtatga gaataggcgg cgcgttcatg aaaatccaca
      661 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgtgtcg
      721 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc gaaggcggtg
      781 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      841 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      901 acgccaaagc gcaaaccgtc caactggaag tcaaaatcgc gccgatgccg gcaatgaacc
      961 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatgtt tgaaatgccg
     1021 tctgaaaaag cccgggcttt cagacggcat ttttatgccc gcctttaaaa tgtgttaaaa
     1081 tccgccttta aaaaccgccg tttccaacca tattctacgc atgaatacga cagcaaacac
     1141 caccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
     1201 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
     1261 cgacgaatat tgcagcatca aacaggattc gttcgatgcc atcgccgttg ccgatattat
     1321 cggcatcgat atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
     1381 ttttcttcag gaatacagtg cggggcgcac gccgaatccg gatgtgttgt gcaacgccga
     1441 aatcaaattc aaatgctttt tggactacgc cgtagggcag ggcgcggata ccatcgccac
     1501 cggacactat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1561 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgagcgcgc
     1621 gatttttccg ttgggcggtt tggaaaaacc cgaagtgcgc cgccttgccg ccgaatttaa
     1681 tttgccgact gccgctaaaa aagacagcac gggcatctgt ttcatcggcg aacgcccgtt
     1741 tcgcgagttt ctgcagaaat acctgccgac cgacaatggt aaaatggtta cgcccgaagg
     1801 gaaaaccgtc ggcgaacacg tcgggctgat gttctacacg ctcggacagc gcaaaggttt
     1861 gggcattggc ggcgcgggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
     1921 actgattgtc gtgcaaggac acgaccatcc gctgctttat acccgcagcc ttgtgatgaa
     1981 cgatttgagt ttcacgctgc ccgaacgccc gaaggcagga cgctatacct gcaaaacgcg
     2041 ttaccgtatg gcggacgcgc cttgtgagtt gcgctatttg gatgatgaaa ccgccgagct
     2101 ggtgtttgac gaaccgcaat gggcggttac gccgggtcag tccgccgtgc tgtacgacgg
     2161 cgatatctgt ttgggcggcg gtatcatcca aacgaccgac aaacccgtca tcatcacacg
     2221 ataaaggtaa tgccgtctga aacggttttc agacggcatt gttccgctca attccacttt
     2281 aaagaccgat acctatgaaa aaaatttggt tagacagcta cgagaagggc gtcagtgccg
     2341 aaatcgacat cacgcaatac aattccgtca gcgacgtatt ccgccaaagc gtggaaaaat
     2401 ttgccgacca acctgctttt caaaacatgg gtaagacgct gacttatgcc gaaaccggca
     2461 aactggcaac tgatttcgcc tcttatctgc aaaacgtcct caagctgccg cgcggcgaac
     2521 gtgttgccat tatgctgccc aacgtattgc aatacccgat tgcccttttc ggtattttgc
     2581 aggcaggttt ggtggcggtg aacaccaatc cgctctatac accgcgcgag ttggagcatc
     2641 agttgaaaga cagcggtgcg accgccatca tcgttttgga aaatttcgcc aacacgctgg
     2701 agctggtgct gccgcgcacg cagatcaaac acgtcatcgt cgcctccgtc ggcgaaatgt
     2761 tcgggctgct taaaggttcg ctgatcaatt tcatcatccg gaaaatcaag aaaatggtgc
     2821 ccgaataccg tattcgggaa accgtttcct ttcagacggc attgaaagaa ggggcgaagc
     2881 acgttttcca acctgtcgca ttaaaccgcg aagataccgc gctgttgcaa tacacgggcg
     2941 gcacgacagg tgttgccaaa ggggcggtgc tgagtcacgg caacatctgc gccaatatgc
     3001 ttcaggcaaa agaatggatt aaaaaccagt tgcgcgaggg caaagaaacc gttatcgccg
     3061 ccttgccgct gtaccatatt ttcgccttaa ccgtgaatct gatgattttt gccaatgccg
     3121 gctcgaaaat catcctgatt accaacccgc gcgatatgaa aggctttatc ggacaactga
     3181 aaaaacagcg ggttaatgta tgtatcggcg tgaacacact gtttaacgca atggttaacc
     3241 gtcctgattt tgccgaagtc gatttttcag gattgcggtt gactttgggc ggcggtatgg
     3301 cgacccaaaa agccgttgcc gaaaaatgga aaaaaatcac cggcacgccc atcgtcgaag
     3361 cctacggtct gaccgaagcc agccccggcg tgtgctgcaa ccccttaaac atcgaatcat
     3421 acagcggcag catcggtttg cccgtctcgt ccaccgaagt cgaactgcgc gacgcaaacg
     3481 gcaaagaagt ccccgttggg cagccgggcg aattgtgggt aaaaggccct caagtgatgc
     3541 aaggctactg gaaacgcccc gaagaaaccg ccaaagccat agacgcgcgc ggctttttgg
     3601 aaaccggcga tattgccgtg atggacgaaa aaggctggtt gaagctggtc gatcgtaaga
     3661 aagacctcgt cgttgtttcc ggattcaatg tttatccgaa cgaaatcgag gaagtcatcg
     3721 cgcatcacgg caaagtcatg gaagtcgcct gtatcggcgt tcccgacgaa aaaaccggag
     3781 aagccctaaa agtattcgtc gtcaaaaaag acccgtcatt gaccaaagaa gaacttatcg
     3841 ccttttgccg caccgaatta accgcatata aagtaccgaa aaatatcgaa ttccgcgacg
     3901 agttgcccaa gtccaacgtc ggcaaaattc tgcgtcgcga gttgcgccaa agtactggga
     3961 aataaagaaa agatgccgtc tgaaaacagc cgtccaccgt tcagacggca tccgcctgtt
     4021 tgccagaacc gcgcgcttca cgttaaaatc acgcattcca atacgggtat ttcattatga
     4081 ccaaattcat tttcgtcacc ggcggcgttg tctcctcact gggtaaaggt atcgccgccg
     4141 cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt taccatgctc aagctcgatc
     4201 cttatatcaa cgtcgatccc ggcacgatga gcccgttcca acacggcgaa gtgttcgtaa
     4261 ccgacgacgg cgcggaaacc gacctcgact tgggacacta cgaacggttt atcgattcca
     4321 cgatgacccg ccgcaacagc ttcagcacgg ggcaggtgta cgaaaacgtc atcgccaaag
     4381 aacgacgggg cgactacctc ggcggcacgg ttcaagtcat cccgcacatt accgacgaaa
     4441 tcaaacgccg catccacgaa ggcgcggcag gttacgatgt ggcgattgtc gaaatcggcg
     4501 gcacggtcgg cgacatcgaa tcgctgccgt ttttggaagc catccgccag atgcgaagcc
     4561 agttgggacg caacaacacc ctgttcgccc acttgagcta cgtcccctac atcgccgctg
     4621 caggcgaaat caaaaccaag ccgacccagc acaccgttaa agaaatgttg agcatcggtt
     4681 tgcaacccga catcctgatt tgccgtatgg acaggacaat gcctgcggac gaacgccgca
     4741 aaatcgcctt gttctgcaac gtggaagaac gcgcgattgt cggcagctac gatgtggaca
     4801 gcatctacga atgccccgaa atgctgcacg accaaggcat cgacaacatc attaccgagc
     4861 agttgcagct taatgtgcaa caggcggatt tgaccgcgtg gaaaaaaatc gtccacgcca
     4921 tccaaaaccc gaaacacacc gtcaaaatcg ccatggtcgg caaatacgtc gatttgaccg
     4981 agtcctacaa atcattgatc gaagccttga aacacgcagg cattcacacc gaaaccgacg
     5041 tacaaattac ctttgtcgat agcgaaagca tcgagaaaaa caacggcgac gtttctatgc
     5101 tcaaagatat ggatgccatc ctcgttcccg gcggtttcgg ttcgcgcggc gtggaaggca
     5161 aaatcgccgc tgtgcgctat gcccgtgaaa acaacgtgcc atacttgggt atctgcctcg
     5221 gtatgcagat tgcgctgatt gaatacgccc gcgacgtggc aggtttgaaa ggtgcgaatt
     5281 ccactgagtt cgacctcaaa tgcgccgccc ccgtcgttgc cctgattgac gaatggcaaa
     5341 ctgccgacgg cagcgtcgaa acccgcgatg aatccgccga tttgggcggc acgatgcgtt
     5401 tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc cgccaaaatc tacggcagcg
     5461 gacatatccg cgaacgccac cgccaccgct acgaagtcaa caacaactat gttcctcagt
     5521 tggaaaaagc aggcttggtt atcggcggcg tatccgccgg acgcgaacgc ttggtcgaaa
     5581 ccatcgagct gccgaaccac ccttggttct tcgcctgcca gttccatccc gaattcactt
     5641 ccaacccgcg caaagggcat cccttgttca ccgcgtttgt caaagctgcg ttgaacaata
     5701 aaaaagcctg ataaagcgtt acttgatgat caaaatgccg tctgaaagcc tgaatgaaga
     5761 ctttcagacg gcattttcgc aaatcgggga ttataaaagc catcaattta atttttatgg
     5821 ttttatctaa tatttaccaa ctgaatattg ttaagcgggg cgtgaaactc aagttgagac
     5881 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttagga gatgttccat
     5941 gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg accgtttccc
     6001 gctattgaag ttggatccag tgattgattg gcagccgatc gaacagtacc tgaatcgtca
     6061 aagaacccgt taccttagag accaccgcgg ccgtcccgcc tatcccctgt tgtccatgtt
     6121 caaagccgtc ctgctcggac aatggcacag cctctccgat tccgaactcg aacacagcct
     6181 catcacccgc atcgacttca acctgtt
//
LOCUS       DAWFIT010000111         6017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFIT010000111 DAWFIT010000000
VERSION     DAWFIT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.111"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            262..1209
                     /locus_tag="WGQ85_001956"
     CDS             262..1209
                     /locus_tag="WGQ85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0751309.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAIAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFEHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1362..2486)
                     /gene="rluD"
                     /locus_tag="WGQ85_001957"
     CDS             complement(1362..2486)
                     /gene="rluD"
                     /locus_tag="WGQ85_001957"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0751310.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2485..3288
                     /locus_tag="WGQ85_001958"
     CDS             2485..3288
                     /locus_tag="WGQ85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0751311.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3346..5565)
                     /locus_tag="WGQ85_001959"
     CDS             complement(3346..5565)
                     /locus_tag="WGQ85_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0751312.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLATFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1487 a         1891 c         1445 g         1194 t
ORIGIN      
        1 aacaggggat ggcacatcaa gcaccgcact gcgtgttgcc cgaacgagac taccctccga
       61 ttagattcta tcgctataaa cagacggatt tcaaccggaa aggaatgggg ataaagtccg
      121 tttccgacac ctctcgggcg ataccgtctg aaaaccaatc tccactttca gacggcattg
      181 tttaggcacg gtcattttgc aaattaaggt taaggcgtgt atgcttgcct tcccttaaaa
      241 acaaacacgc aacgcattat tatgaatatc ctcagtaaaa tcagcagctt tatcggaaaa
      301 acattttccc tctgggccgc gctctttgcc gccatcgctt ttttcgcgcc cgacaccttc
      361 aaatgggcgg ggccttatat tccttggctg ttgggcatta ttatgttcgg tatgggtttg
      421 acgctcaaac cttccgactt cgatattttg ttcgaacatc ccaaagtcgt catcatcggc
      481 gtaatcgcac aattcgccat tatgccggca accgcctggc tgctgtccaa actgttgaac
      541 ctgcctgccg aaatcgcggt cggcgtgatt ttggtcggct gctgcccggg cggtacggct
      601 tccaatgtga tgacctatct ggcgcgcggc aatgtggctt tgtcggttgc cgttacgtct
      661 gtttccaccc tgatttcccc attgctgact cccgccatct tcctgatgct tgccggtgaa
      721 atgctggaaa tccaagcggg cagtatgttg atgtccatcg tcaaaatggt tttgctcccc
      781 attgttttgg gtttgattgt ccataaggtt ttgggcagta aaaccgaaaa gctgaccgat
      841 gcgctgccgc tggtttccgt tgccgccatc gtgctgatta tcggcgcggt tgttggggca
      901 agcaaaggca agattatgga aagcggcctg ctgatttttg cggttgtcgt actccacaac
      961 ggcatcggct acctgctcgg cttctttgcc gccaaatgga ccggcctgcc ttatgatgca
     1021 caaaaaacgc tgaccatcga agtcggtatg caaaactcgg gcctggccgc cgcgcttgcc
     1081 gccgcacact ttgccgccgc gccggtcgtt gccgttccgg gcgcattgtt cagcgtgtgg
     1141 cacaatatct ccggctcgct gctggcaact tattgggcgg ccaaagccgg taaacataaa
     1201 aaaccctaaa cgcctttcat cataatgccg tctgaacggg gcaaacaaaa ctggcggttt
     1261 ctgcccggcc ccttcagacg gcctttgttt gaaatcggcg gggacgattg cccggctgct
     1321 ccgattcggc tgcctgcgtt cgccccgcct ttcaagccgc ctcactccct tacataaacc
     1381 acttcgacat cgtagtcgtc ttcgttccaa tcgtcatcgt cgtccgcgcc gaatttgtcc
     1441 tgccattcct cttcattgct caaagacgaa tccaaaccgg cttcaagacg gaggacagac
     1501 aacaaatgat atatgtcgtc cggaatcggt gcttcaaacg aaacggtttc gccggttttc
     1561 ggatgagtga aacttaagcg gtaggcgtgc aatgcctgac gcgcacccaa acttttaacg
     1621 gcttctttca ccgtgtcgcc gcacggatgg cgcgggttgc cgtaaaccgg gtcggcggca
     1681 agcggatggt tggcctcacg catatggacg cggatttggt gcgtcctgcc tgtttcgagc
     1741 gagcattcga tatagctgtg cgccagatag cgttccaaca ctttgacgtg ggtaacggct
     1801 ggtttgccgc cgaatttgac gactgccatt ttcaggcggt tgtgcggatc gcgtccgatt
     1861 tgggtttcga ttttaccgtc gaaggggacg atgccattgg cgacggcgcg gtagatgcgt
     1921 ttgaccgtgc gctcttgaag ctgcctcacg agggaatttt gcgccgacag ggttttggca
     1981 accaccatca gcccgctggt ttctttgtcc aaacggtgta cgatgcccgc gcgcggaatt
     2041 tgggacagct cggggcaatg cgccaacagg ccgttgagca gcgtccccgt ccagttgccc
     2101 gccgccggat gcaccaccag tccggccggt ttgttgacga cgatgacggt atcgtcttcg
     2161 taaacgatat ccaaagccat aggctctgga acaaacgcca gattttcctc actcggacgg
     2221 acggttacac aaatttgctc gccgcctatc attttgtctt tgggttgcga aggtttatcg
     2281 tttacaataa ccgcgccttc tttaatccat gatgtcaggc ggctgcgcga gtagtcgggc
     2341 agaagtttcg ccaacaccgc atccaaccgc ccgccggcaa gctcgagcgg aacggtcaga
     2401 ccgatacggt tttcagtttc ggaagctgac gcaaagtctg aatcatcgct ataatcggat
     2461 tcattatcaa aggaagtatt ctgcatgaaa aaaattcttt taacggtttc attaggtttg
     2521 gcactgagtg cctgtgccac tcaaggtacg gtcgataaag atgctcagat tacccaagat
     2581 tggagtgtgg agaagctcta tgccgaagcc caggacgagc tgaacagcag caattatacg
     2641 cgagctgtca aattatacga aatcttggaa tcgcgcttcc ctaccagccg ccatgcccgg
     2701 caatcccaac tggataccgc atacgcctat tataaagacg atgaaaaaga caaagctctg
     2761 gcggcaatcg aacgcttccg ccgccttcac ccgcaacacc cgaatatgga ttacgcgctg
     2821 tatctgcgcg gcttggtgct gttcaacgaa gaccagtcct tcttgaacaa actggcctcg
     2881 caagactggt ccgaccgcga cccgaaagcc aaccgcgaag cgtaccaggc gtttgcggaa
     2941 ctcgtccaac gcttccccaa cagcaaatac gccgccgatg caaccgcacg catggtcaaa
     3001 ctggtcgatg cactgggcgg caatgaaatg tcggtggcgc gctactacat gaaacgcggt
     3061 gcatacatcg ccgccgccaa ccgcgcccaa aaaatcatcg gcagctacca gaatacgcgt
     3121 tatgtcgaag aatcgctcgc catattggaa cttgcctacc aaaaactcgg caaaccacag
     3181 cttgccgccg atacgcgccg cgtgttggaa accaacttcc cgaaaagccc gtttttgacg
     3241 cacgcttggc agcccgacga tatgccctgg tggcgttact ggcattaagc ccatcttcgt
     3301 aatgccgtct gaagccgttt cagacggcat tttatgtttc aaacctcatt caaacggttt
     3361 tttctgccaa tagaatttat accctctcaa acgttgagcc ttcacgccgc cgcgtcccaa
     3421 gccgaattgc agtgcgcccg acaaatcggt acgcagcagt ttgatgccgt gtgcgcggac
     3481 acggttctgc accgcttcgg tcggatgttt gtaggcgttg gcataaccgc ttgaagcaac
     3541 ggcatattcg ggcgagacgg cattgaggaa cacgcccgac gaggacgtat tgctgccgtg
     3601 atgtcccaac accaaaacct ggctgtacag gttgcctcca tacttgccga ccaggctttc
     3661 ctcgcccttc gtatccaaat cgcccgttac cagcagtgcc gcaccgcccg ccacaacacg
     3721 caaaacacaa cttttcccat tatcatcgat gtttttgcgt tcagacggca tcaaaaactc
     3781 aaaatccacg ccgtcccatt gccaacgctg ttccgcacaa tgccgcgccc cctcataaaa
     3841 ttccggctgt ccggcataaa tcccgccgtt cggtatcttg ccgactgcct gaaaaccgcc
     3901 gtcgtggtcg ctgtcgtgat gcgacagaac cagcttgtcc aaacggcgga cacccgccgc
     3961 attcaaactc ggcacaatcc ccatctgcgc cgccgctgcc gttccggtgt caaacaaaag
     4021 atgatgattt gccgtctgaa ccgacaccga caaaccctgc cccgcatccc aaaccgtaac
     4081 cgcaacctca ttttccggca cgccgggtga acggtaaaac acaaaccctg ccaacagcaa
     4141 caccgcccac ggacgcaaac ccaagccgcg cggcagcagc aacagcaaag cggcacacac
     4201 cgccaacacc aacagcggca aaggtgcgga ggcaacggca aactcgggcg acacatcggc
     4261 aagccacacc aaaaaccgca aagtatattc cgccaaaaat gcccccaact gttgcaaagg
     4321 cgcaaacggc acgaccgaac ccagcaacgc cagcggcgtc aatacccaag aaaaccacgg
     4381 aattgccacc gcattgacca aagggctgac caaaggcagc gaagcaaaca gataaccgag
     4441 caaaaccagc gacaacaccg aagccgccca ctgcccgcgc aaagcagttt gccgttttcc
     4501 ctcgtgcaaa cgccccgagc acgcccatat cagggccgcc accaaaccga aagacagcca
     4561 agtccccaca cccaagaccg ccgacgggtc gaacagcagc accgccgcca acgcctgcca
     4621 ccacgtcgcc cacgccgaca atcttcccct gcgccaagcc cacgcaaacg tcgccaacat
     4681 caaaacgctg cgctgcgtcg gcacggaaaa accggcaagc agcgcgtaaa acagcgcgcc
     4741 tgcacacccc gccgccaaaa cccacgcgcg cggccgggca ggaaggcgcg gggaacaggc
     4801 aagcagccgc ttcgccagcc acgcaaacat taccgccacc atcgtaacgt gcaaacccga
     4861 aatactgacc aaatgcgtca gccccaacgg ccggaacgcc tgccacaatt cggggcgcaa
     4921 tgccgactgt tcgcccacgc tcaacgcgcg catcagcccg atgccgtctg aaagcccgcc
     4981 gtccgcatcc gcctgctgcc aattacggct gatgcggctg cgccaaaccg caatccccca
     5041 accgcttccg ccatgcagca aaaccctgtc cgcaccgacc gtccccgcgc cgcctattcc
     5101 gttggataat gcccacgcct cacggttcaa accgcgcagg ttcaactccc cgacgacagg
     5161 gtgcacacgt gccgttatcc gccatctgct cccgaccgcc cattcgcgcc gtttgtagtc
     5221 cgacagcagc aaatcaaacg tccgaccacc gctgtccaca gcctttgccg caaactgcac
     5281 acgccgcccg tccgacctcg gcatatccgc cacttccacc gtcaaaggca cgcctgaaac
     5341 ggcttctgcc caccattggg aagacaatgc cgcctccgtc ctgaatacgc cgtaagccgc
     5401 gcccgccaac acgcacagca tcagaccggc aaacgcaaaa cgccttgcaa acacagcaaa
     5461 aaccgcaaaa gccgccagcc aaaacagcca atgcggcacg accggcagcg cgaacgaagc
     5521 cgccgcaccg acaacccaac acggcaaaaa ccaacgacgg aacacgtctc cccaccccag
     5581 atatttcaaa aataaacgga taataaagca aatccgaaaa accgtcataa caagatttga
     5641 aaatgatata gtggtttaaa tttaaatcag gacaaggcga cgaagccgca gacagtacaa
     5701 gcagtacgac aaggcgaggc aacgctgtac tggtttttgt taaaccacta taagaatgca
     5761 aacgaaaagc ctcagtatta tccgagacaa cggcaaactt tccatcgtca ttcccgcaca
     5821 agcgggaatc caaaaacaaa aagccgcaga aatttaacgg aagaaccaaa ccccgaaaac
     5881 cggctccccg ccttcgcttg aataacagaa caaaggaatc agtattatcc gagacaaccg
     5941 caaactttcc atcgtcattc ccgcacaagc gggaatccaa acttgtccgc acggaaactt
     6001 atcggataaa acggttt
//
LOCUS       DAWFIT010000112         5896 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFIT010000112 DAWFIT010000000
VERSION     DAWFIT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.112"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..317)
                     /locus_tag="WGQ85_001960"
     CDS             complement(<1..317)
                     /locus_tag="WGQ85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0751313.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRA"
     gene            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGQ85_001961"
     rRNA            complement(353..466)
                     /gene="rrf"
                     /locus_tag="WGQ85_001961"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(563..3454)
                     /locus_tag="WGQ85_001962"
     rRNA            complement(563..3454)
                     /locus_tag="WGQ85_001962"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3861..3936)
                     /locus_tag="WGQ85_001963"
     tRNA            complement(3861..3936)
                     /locus_tag="WGQ85_001963"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3901..3903),aa:Ala,seq:tgc)
     gene            complement(3942..4018)
                     /locus_tag="WGQ85_001964"
     tRNA            complement(3942..4018)
                     /locus_tag="WGQ85_001964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3982..3984),aa:Ile,seq:gat)
     gene            complement(4120..5660)
                     /locus_tag="WGQ85_001965"
     rRNA            complement(4120..5660)
                     /locus_tag="WGQ85_001965"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1280 a         1725 c         1262 g         1629 t
ORIGIN      
        1 tgcgcacggt attccgcaat ggccttcgcc ttcgcagggc ctatgcccgg cagcgcctcc
       61 agctcctgct gcgaagccgc attgatgttt accgccgcaa gggagaaggc gcaggagaac
      121 agcatacaga acagcacgaa cattttcttc atggtttttc ctttaagggt tgcaaacaat
      181 aaaccgcatc ttgcgacgat aaaacgggtc attctaaaat gaatatccca aagtttcaag
      241 ccgttcctcc gcaaacccga ccggacgccg tacggatgcc gtcccgccat ccccgacatt
      301 ttttccgggc aaagcaaaaa cccccgaata atcgggggtt ttctgaatgg gtgtttggca
      361 gtgacctact ttcgcatgga agaaccacac tatcatcggc gctgagtcgt ttcacggtcc
      421 tgttcgggat gggaaggcgt gggaccaact cgctatggcc gccaaactta aactgttaca
      481 aatcggtaaa gccttaatca atatattcgg taatgactga atcagtcagt aagcttttat
      541 ctcttgaagt tcttcaaatg atagagtcaa gcctcacgag caattagtat gggttagctt
      601 cacgcgttac cgcgcttcca caccccacct atcaacgtcc tggtctcgaa cgactcttta
      661 gtgcggttaa accgcaaggg aagtctcatc ttcaggcgag tttcgcgctt agatgctttc
      721 agcgcttatc tcttccgaac ttagctaccc ggctatgcaa ctggcgttac aaccggtaca
      781 ccagaggttc gtccactccg gtcctctcgt actaggagca gcccccgtca aacttccaac
      841 gcccactgca gatagggacc aaactgtctc acgacgtttt aaacccagct cacgtaccac
      901 tttaaatggc gaacagccat acccttggga ccgactacag ccccaggatg tgatgagccg
      961 acatcgaggt gccaaactcc gccgtcgata tgaactcttg ggcggaatca gcctgttatc
     1021 cccggagtac cttttatccg ttgagcgatg gcccttccat acagaaccac cggatcacta
     1081 tgtcctgctt tcgcacctgc tcgacttgtc ggtctcgcag ttaagctacc ttttgccatt
     1141 gcactatcag tccgatttcc gaccggacct aggtaacctt cgaactcctc cgttacgctt
     1201 tgggaggaga ccgccccagt caaactgcct accatgcacg gtccccgacc cggatgacgg
     1261 gtctgggtta gaacctcaaa gacaccaggg tggtatttca aggacggctc cacagagact
     1321 ggcgtctctg cttctaagcc tcccacctat cctacacaag tgacttcaaa gtccaatgca
     1381 aagctacagt aaaggttcac ggggtctttc cgtctagcag cgggtagatt gcatcttcac
     1441 aaccacttca acttcgctga gtctcaggag gagacagtgt ggccatcgtt acgccattcg
     1501 tgcgggtcgg aacttacccg acaaggaatt tcgctacctt aggaccgtta tagttacggc
     1561 cgccgtttac tggggcttcg atccgatgct ctcacatctt caattaacct tccagcaccg
     1621 ggcaggcgtc acaccctata cgtccacttt cgtgttagca gagtgctgtg tttttaataa
     1681 acagtcgcag ccacctattc tctgcgaccc tccggggctt acggagcaag tccttaacct
     1741 tagagggcat accttctccc gaagttacgg tatcaatttg ccgagttcct tctcctgagt
     1801 tctctcaagc gccttagaat tctcatcctg cccacctgtg tcggtttgcg gtacggttcg
     1861 attcaaactg aagcttagtg gcttttcctg gaagcgtggt atcggttgct tcgtgtccgt
     1921 agacactcgt cgtcacttct cggtgttaag aagacccgga tttgcctaag tcttccacct
     1981 accggcttaa acaagctatt ccaacagctt gccaacctaa ccttctccgt ccccacatcg
     2041 catttgaatc aagtacagga atattaacct gtttcccatc gactacgcat ttctgcctcg
     2101 ccttaggggc cgactcaccc tacgccgatg aacgttgcgc aggaaacctt gggctttcgg
     2161 cgagcgggct tttcacccgc tttatcgcta ctcatgtcaa cattcgcact tctgatacct
     2221 ccagcacact ttacaatgca ccttcatcgg cctacagaac gctcccctac catgccggta
     2281 aaccggcatc cgcagcttcg gttatagatt tgagccccgt tacatcttcc gcgcaggacg
     2341 actcgaccag tgagctatta cgctttcttt aaatgatggc tgcttctaag ccaacatcct
     2401 ggctgtctgg gccttcccac ttcgtttacc acttaatcta tcattgggga ccttagctgg
     2461 cggtctgggt tgtttccctc ttgacaacgg acgttagcac ccgctgtctg tctcccgagg
     2521 aaccacttga tggtattctg agtttgccat gggttggtaa gttgcaataa ccccctagcc
     2581 ataacagtgc tttaccccca tcagtgtctt gctcgaggca ctacctaaat agttttcggg
     2641 gagaaccagc tatctccgag tttgtttagc ctttcacccc tatccacagc tcatccccgc
     2701 attttgcaac atgcgtgggt tcggtcctcc agtacctgtt acggcacctt caacctggcc
     2761 atggatagat cactcggttt cgggtctaca cccagcaact catcgcccta ttaagactcg
     2821 gtttccctac gcctccccta ttcggttaag ctcgctactg aatgtaagtc gttgacccat
     2881 tatacaaaag gtacgcagtc acacctctag ggcgctccca ctgtttgtat gcatcaggtt
     2941 tcaggttctg tttcactccc ctcccggggt tcttttcgcc tttccctcac ggtactggtt
     3001 cactatcggt cgatgatgag tatttagcct tggaggatgg tccccccata ttcagacagg
     3061 atttcacgtg tcccgcccta cttttcgtac gcttagtacc actattgaga tttcgaatac
     3121 gggactatca cccactatgg tcaagcttcc cagcttgttc ttctatctcg acagttatca
     3181 cgtacaggct cctccgcgtt cgctcgccac tacttgcgga atctcggttg atttcttttc
     3241 ctccgggtac ttagatggtt cagttctccg ggttcgcttc tctaagtcta tgtattcaac
     3301 ttaggatact gcacagaatg cagtgggttt ccccattcgg acatcgcgga atcattgctt
     3361 tattgccagc tcccccgcgc ttttcgcagg cttacacgtc cttcgtcgcc tatcatcgcc
     3421 aaggcatcca cctgatgcac ttattcactt gactctatca tttcaagaac ttctttgact
     3481 ttgcctaaca ttccgttgac tagaacatca gacttgaatt tcctactttg ataaagctta
     3541 ctgctttgtt gtgtcttaat cctgcctttt gtgtttcagg attaagtcga tacaatcatc
     3601 acccaaatac tgtgtttgtt ttcttttctc ttgtgagcga ttttttatcc tttgcaaaga
     3661 ataaaaaatc aaaacaaacg ctttgtcttt gtttgttgat ttcggctttc caatttgtta
     3721 aagatcgatg cgttcgatat tgctatctac tgtgcaaatc aaaacgagct gattattata
     3781 tcagcatttt gttcttggtc aagtgtgacg tcgccctgaa tggattctgt tccattcttc
     3841 cgttttgatt tgtacagtat tggtggaggc aaacgggatc gaaccgatga ccccctgctt
     3901 gcaaagcagg tgctctacca actgagctat gcccccgttc ttggtgggtc tgggaggact
     3961 tgaacctccg accccacgct tatcaagcgt gtgctctaac cagctgagct acaaacccgg
     4021 attctcttct taagcgaatc ttgccttcac tcaagcttct tccgcatctt tttcagttta
     4081 ccgataagtg tgaatgccta aagcctcttc tttctctaga aaggaggtga tccagccgca
     4141 ggttccccta cggctacctt gttacgactt caccccagtc atgaagcata ccgtggtaag
     4201 cgggctcctt gcggttaccc tacctacttc tggtatcccc cactcccatg gtgtgacggg
     4261 cggtgtgtac aagacccggg aacgtattca ccgcagtatg ctgacctgcg attactagcg
     4321 attccgactt catgcactcg agttgcagag tgcaatccgg actacgatcg gttttgtgag
     4381 attggctccg cctcgcggct tggctaccct ctgtaccgac cattgtatga cgtgtgaagc
     4441 cctggtcata agggccatga ggacttgacg tcatccccac cttcctccgg cttgtcaccg
     4501 gcagtctcat tagagtgccc aactgaatga tggcaactaa tgacaagggt tgcgctcgtt
     4561 gcgggactta acccaacatc tcacgacacg agctgacgac agccatgcag cacctgtgtt
     4621 acggctcccg aaggcactcc tccgtctccg gaggattccg tacatgtcaa gaccaggtaa
     4681 ggttcttcgc gttgcatcga attaatccac atcatccacc gcttgtgcgg gtccccgtca
     4741 attcctttga gttttaatct tgcgaccgta ctccccaggc ggtcaatttc acgcgttagc
     4801 tacgctacca agcaatcaag ttgcccaaca gctaattgac atcgtttagg gcgtggacta
     4861 ccagggtatc taatcctgtt tgctacccac gctttcgggc atgaacgtca gtgttatccc
     4921 aggaggctgc cttcgccatc ggtattcctc cacatctcta cgcatttcac tgctacacgt
     4981 ggaattctac ctccctctga cacactcgag tcacccagtt cagaacgcag ttcccaggtt
     5041 gagcccgggg atttcacatc ctgcttaagt aaccgtctgc gcccgcttta cgcccagtaa
     5101 ttccgattaa cgctcgcacc ctacgtatta ccgcggctgc tggcacgtag ttagccggtg
     5161 cttattcttc aggtaccgtc atcagccgct gatattagca acagcctttt cttccctgac
     5221 aaaagtcctt tacaacccga aggccttctt cagacacgcg gcatggctgg atcaggcttg
     5281 cgcccattgt ccaaaattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt
     5341 cccagtgtgg cggatcatcc tctcagaccc gctactgatc gtcgccttgg taggccttta
     5401 ccccaccaac tagctaatca gatatcggcc gctcgaatag cgcaaggccc gaaggtcccc
     5461 tgctttctct ctcaagacgt atgcggtatt agctgatctt tcgatcagtt atcccccact
     5521 actcggtacg ttccgatatg ttactcaccc gttcgccact cgccacccaa gaagcaagct
     5581 tctctgtgct gccgtccgac ttgcatgtgt aaagcatgcc gccagcgttc aatctgagcc
     5641 aggatcaaac tcttatgttc aatctctaac tttttaactt ctggtctgct tcaaagaaac
     5701 caacaggaca atgttcaaaa cattatcttg tctgtctttc aaacagtgtg aggctcaagg
     5761 cactcacact tatcggtaat ctgttttgtt aaagagcgtt tgaattataa agtatttctt
     5821 ccgcctgtca agatatctct cgatatcccc aacattctgt gctatacttt tcagttcgtc
     5881 cgccacttct gcagca
//
LOCUS       DAWFIT010000113         5860 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFIT010000113 DAWFIT010000000
VERSION     DAWFIT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.113"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            45..452
                     /locus_tag="WGQ85_001966"
     CDS             45..452
                     /locus_tag="WGQ85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0751314.1"
                     /translation="MMPAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGALLC
                     AGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLAAG
                     WLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            498..1127
                     /gene="nth"
                     /locus_tag="WGQ85_001967"
     CDS             498..1127
                     /gene="nth"
                     /locus_tag="WGQ85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0751315.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1265..2764
                     /locus_tag="WGQ85_001968"
     CDS             1265..2764
                     /locus_tag="WGQ85_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0751316.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2966..4486
                     /locus_tag="WGQ85_001969"
     CDS             2966..4486
                     /locus_tag="WGQ85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0751317.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSWGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISMVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4543..5628
                     /gene="nagZ"
                     /locus_tag="WGQ85_001970"
     CDS             4543..5628
                     /gene="nagZ"
                     /locus_tag="WGQ85_001970"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0751318.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5709..5785
                     /locus_tag="WGQ85_001971"
     tRNA            5709..5785
                     /locus_tag="WGQ85_001971"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5743..5745,aa:Pro,seq:cgg)
BASE COUNT         1343 a         1801 c         1474 g         1242 t
ORIGIN      
        1 cgtacaatcc gtcgccgatt tttgcctgtt tgagactgtt tgcaatgatg cccgcctacg
       61 ccgcgctcgc cttttccgcc ttcacttccg ccaccctcct gcccggcaca tccgaagccg
      121 cctttgccct gttcgtccac cgctttcccg aacacgcata cggcgcgttg ctgtgcgccg
      181 gccttgccaa cggattgggc agtatggttt cctattggat ggggcgtttg ctgccctccc
      241 gaaaaatgcc gtctgaaaaa acactgaatc tgatgcggcg tttcggtatt tggctgcttg
      301 cgtttgcctg gctgcccgtc gtcggcgacg cactgccgct tgccgccggc tggctgcggt
      361 tgaatccgtg gacaagcggg ctgatgctgg ttatcggcaa aacggcgcgt tacgccttca
      421 ttctgtgggg aatgcaatat tacgccgcct gaaccgtgcg ttataatgcc gaccgcacaa
      481 accgactgca caaaaaaatg aacagacaca tccgccaaga aattttcgaa cgcttccgcg
      541 ccgccaaccc ccatccgacc accgagctga atttcaactc ccctttcgag cttttaatcg
      601 ccgttctgct ttcggcgcag gcgaccgatg tcggcgtaaa caaggcgacg gcgaagctgt
      661 ttccggttgc cgatacgccg caggcaatgc tggatttggg cttggacggc gtgatggaat
      721 acacgaaaac catcgggttg tataaaacca agtccaagca catcatgcag acctgccgca
      781 tcctgctgga aaaatacaag ggcgaagtgc cggaagaccg cgaggctttg gaatcgttgc
      841 cgggcgtggg gcgcaaaacg gcaaacgtgg tattgaacac agcgttcgga caccccgtta
      901 tggcggtcga tacgcatatt ttccgcgtgt ccaaccgaac caaaatcgca cccgggaaag
      961 atgtgcgcga agtcgaagac aaactgatgc gcttcattcc taaagaattt ctgatggacg
     1021 cgcaccactg gctgattttg cacggacgct acacctgcaa ggcactcaaa ccgcaatgcc
     1081 aaacctgcat catcaacgat ttgtgcgaat atcccgccaa agcctgactg ccgccgccct
     1141 tcaaaatgaa acgcttgaaa attcggacgg acgttgtaga atttcaacct atgccgtctg
     1201 aagccgacaa aatgcgcttc agacggcatt aaagccggta ccggcatccg acaaaggaca
     1261 aaacgtgttc aaaaaatacc aatacctcgc tttggcagca ctgtgtgccg cctcgctggc
     1321 aggctgcgac aaagccggca gctttttcgg tgcggacaaa aaagaagcat ccttcgtaga
     1381 acgcatcaaa cacaccaaag acgacggcag cgtcagtatg ctgctgcccg actttgtcca
     1441 actggttcaa agcgaaggcc cggcagtcgt caatattcag gcagcccccg ccccgcgcac
     1501 ccaaaacggc agcagcaatg ccgaaaccga ttccgacccg cttgccgaca gcgacccgtt
     1561 ctacgaattt ttcaaacgcc tcgtcccgaa catgcccgaa atcccccaag aagaagcaga
     1621 tgacggcgga ttgaacttcg gttcgggctt catcatcagc aaagacggct atattctgac
     1681 caatacgcac gtcgttaccg gcatgggcag tatcaaagtc ctgctcaacg acaagcgcga
     1741 atataccgcc aaactcatcg gttcggatgt ccaatccgat gtcgcccttc tgaaaatcga
     1801 cgcaacggaa gagctgcccg tcgtcaaaat cggcaatccc aaagatttga aaccgggcga
     1861 atgggtcgcc gccatcggcg cgcccttcgg cttcgacaac agcgtgaccg ccggcatcgt
     1921 gtccgccaaa ggcagaagcc tgcccaacga aagctacaca cccttcatcc aaaccgacgt
     1981 tgccatcaat ccgggcaact ccggcggtcc gctgttcaac ctgaaaggac aggtcgtcgg
     2041 catcaactcg caaatataca gccgcagcgg cggatttatg ggcatctcct ttgccatccc
     2101 catcgacgtt gccatgaatg ttgccgaaca gctgaaaaac accggcaaag tccaacgcgg
     2161 gcaactgggc gtgattattc aagaagtatc ctacggtttg gcacaatcgt tcggtttgga
     2221 caaagccggc ggcgcactga ttgccaaaat cctgcccggc agccccgcag aacgtgccgg
     2281 cctgcaggcg ggcgacatcg tcctcagcct cgacggcgga gaaatacgtt cttccggcga
     2341 ccttcccgtt atggtcggcg ccattacgcc gggaaaagaa gtcagcctcg gcgtatggcg
     2401 caaaggcgaa gaaatcacaa tcaaagtcaa gctgggcaac gccgccgagc atatcggcgc
     2461 atcatccaaa acagatgaag ccccctacac cgaacagcaa tccggtacgt tctcggtcga
     2521 atccgcaggc attacccttc agacacatac cgacagcagc ggcggacacc tcgtcgtcgt
     2581 acgggtttcc gacgcggcag aacgcgcagg cttgaggcgc ggcgacgaaa ttcttgccgt
     2641 cgggcaagtc cccgtcaatg acgaagccgg tttccgcaaa gctatggaca aggcaggcaa
     2701 aaacgtcccc ctgctgatca tgcgccgtgg caacacgctg tttatcgcat taaacctgca
     2761 ataacacaca taatttaacc aaaatatttt ttaaatgata aaatgcccgt caataccgat
     2821 taacgggcat tttacaaaaa tcagttggtt gattttaaac aaatattttt tacgacaaaa
     2881 aatatttccg cccattttgc caacaaaacc caaaccggag gcggacagat acgctcccat
     2941 ccggacgctt aacaccccca taaccatgca gctgatcgac tattcacatt catttttctc
     3001 ggttgtgcca ccctttttgg cactggcact tgccgtcatt acccgccgcg tactgctgtc
     3061 tttaggcatc ggtattctgg tcggcgttgc ctttttggtc ggcggcaacc ccgtcgacgg
     3121 tctgacacac ctgaaagaca tggtcgtcgg cttggcttgg tcagacggcg attggtcgct
     3181 gggcaaacca aaaatcttgg ttttcctgat acttttgggt atttttactt ccctgctgac
     3241 ctactccggc agcaatcagg cgtttgccga ctgggcaaaa cggcacatta aaaaccggcg
     3301 cggcgcgaaa atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg actatttcca
     3361 cagtctcgcc gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag tttcccgcgc
     3421 caaactcgcc tacatcctcg actccactgc cgcgcctatg tgcgtgctga tgcccgtttc
     3481 aagctggggc gcgtcgatta tcgccacgct tgccggactg ctcgttacct acaaaatcac
     3541 cgaatacacg ccgatgggga cgtttgtcgc catgagcctg atgaactatt acgcactgtt
     3601 tgccctgatt atggtgttcg tcgtcgcatg gttctccttc gacatcggct cgatggcacg
     3661 tttcgaacaa gccgcgttga acgaagccca cgatgaaact gccgtttcag acggcagctg
     3721 gggcagggtt tacgcattga ttattcccgt tttggcctta atcgcctcaa cggtttccgc
     3781 catgatctac accggtgcac aggcaagcga aaccttcagc attttggggg catttgaaaa
     3841 taccgacgtg aacacttcgc tggtattcgg cggcacttgc ggcgtccttg ccgtcgttct
     3901 ctgcacgctc ggcacgatta aaaccgccga ctatcccaaa gccgtttggc agggtgcgaa
     3961 atccatgttc ggcgcaatcg ccattttaat cctcgcttgg ctcatcagta tggttgtcgg
     4021 cgaaatgcac accggcgatt acctctccac actggttgcg ggcaacatcc atcccggctt
     4081 cctgcccgtc atcctcttcc tgctcgccag cgtgatggcg tttgccacag gcacaagctg
     4141 ggggacgttc ggcattatgc tgccgattgc cgccgccatg gcggtcaaag tcgaacccgc
     4201 gctgattatc ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg accactgttc
     4261 gcccatctcc gacacgacca tcctgtcgtc cacaggcgcg cgctgcaacc acatcgacca
     4321 cgttacctcg caactgcctt acgccttaac cgttgccgcc gccgccgcat cgggctacct
     4381 cgcattgggt ctgacaaaat ccgcgctgtt gggctttggc acgacaggca ttgtattggc
     4441 ggtgctgatt tttctgttga aagataaaaa acgcgccaac gcctgacccc tttccctgtt
     4501 cagacggcat atgccgtctg aagctttttg gagcaaaccg caatgaccgt cccccatatt
     4561 ccgcgcggcc ccgtcatggc agacattgcc gccttccgcc tgaccgaaga ggaaaagcaa
     4621 cgcctgctcg atcccgccgt aggcggcatc atcctcttcc gccgcaactt ccaaaacatc
     4681 gaacaactca aaacactcac cgccgaaatc aaagccctgc gcacacccga actcattatc
     4741 gccgtcgatc acgaaggcgg cagggtgcaa cgtttcatcg aaggcttcac ccgcctgccc
     4801 gcaatgaacg ttttagggca aatttgggac aaagacggcg cgtccgccgc cgaaaccgcc
     4861 gccggacaag tcggccgggt gttggcaacc gagctttccg cctgcggcat agacctctcc
     4921 ttcacccccg ttctcgactt ggactgggga caatgcgccg tcatcggcaa ccgcagtttc
     4981 caccacaatc ccgaagcggt cgcccacctc gccctcgccc ttcaaaaagg tttggcaaaa
     5041 ggcggtatga aatcctgcgg caaacatttt cccggacacg gatttgtcga aggagacagc
     5101 catctggtct tgccggaaga caggcgcagc ctgtccgaac tcgaagccgc cgacctcgcc
     5161 cccttccaca ttatgagccg cgaaggtatg gcggcggtta tgcccgccca cgtcgtttat
     5221 ccacaagtgg acacaaagcc cgcagggttt tccgaaatct ggctcaaaca aattttgcgc
     5281 cgcgatatcg gattcaaagg cgtgattttc tcggacgatt tgactatgga aggcgcgtgc
     5341 ggggcaggcg gcatcaaaga acgcgcccgc atttcctttg aggcaggttg cgacatcgtc
     5401 ctcgtctgca accgtcccga ccttgtggat gaactgcgcg aagatttccg cattcctgac
     5461 aatcccgctt tggcgcaacg ttggcaatat atggccaaca cgctcggcag tgccgccgcg
     5521 caagccgtta tacagacggc ggatttccaa gcggcgcaag cctttgttgc cggccttgcc
     5581 tcgccgcaag acacggcggg cggcgtaaaa gtcggcgaag ccttttaaat ccgcccgaca
     5641 cgcggcaaaa aggcaaaacg gtataggcac gccgtatttt ttgcgctata atcacgcctt
     5701 acttaattcg gaatgtagcg cagcccggta gcgcacttcg ttcgggacga aggggtcgga
     5761 ggttcgaatc ctctcattcc gaccaaatcc aaaccgcctc ggtatccatt gccgaggcag
     5821 tttttccttt tgacgtcaac agaaaaatgc cgtctgaaac
//
LOCUS       DAWFIT010000114         5573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFIT010000114 DAWFIT010000000
VERSION     DAWFIT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.114"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGQ85_001972"
     CDS             complement(380..3121)
                     /gene="tbpA"
                     /locus_tag="WGQ85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0751319.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTEQICRLGNNT
                     YTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLGKI
                     DWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3207..5372)
                     /locus_tag="WGQ85_001973"
     CDS             complement(3207..5372)
                     /locus_tag="WGQ85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0751320.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPTAMPKENEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISNVQTDNGDNNIYISPYLTQSSHNSHNGSINGGANQPKNEATGYNNFKYV
                     YSGWFYKHAASERKFNEGKFKSGDDGYIFYHGKDPSRQLPASGKVTYKGVWHFVTDTK
                     LGQKFNDIIQPSKKQGDSYSGFSGDEGEEYSNKNESTLKDGHEGYGFTSNLEVDFGNK
                     KLTGKLIRNNASQSNTNNDKPTTQYYSLEAQVTGNRFNGKAIATDKPQENETKQHPFV
                     SDSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKPRNGAVASGGTGAAASDG
                     AAGTSSKNGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QANQGTNGGTAFTYKTTYTLESDKKDTKAQTGAGGAQAASGAAGVNGGQAGTKTYEVE
                     VCCSNLNYLKYGMLTRKNSKSAMQAGENGSQADAKTEQVEQSMFLQGERTDEKEIPKE
                     QQDIVYRGSWYGHIANNTSTSWSGNASNATSGNRADFTVNFGDKKITGTLTANDRTQP
                     TFTITANIKDNGFEGTAKTAELGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGG
                     WFAYSGDKQTENTTVASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         1160 a         1417 c         1386 g         1610 t
ORIGIN      
        1 cccatcattc ccgcgcagga gggaatccga atacatccga acagaaacct gcatcccgtc
       61 attcccgcga aagcgggaat ctagaacgta aaatctaaag aaaccgtttt atccgataag
      121 tttccgcacc gacaggtcta gattcccact ttcgcgggaa tgacgggatt tgaggtttct
      181 gtttttgatt ttctgttttc gtaggaatga cggtttagag ttacccgaaa cctcaaaaaa
      241 aaccgaaacc gaacggactg gattcccgcc tgcgtgggaa tgacgggttt caagattacg
      301 gtattgtcgg ggatgatggg aaacggtggg aattgtgtaa aaaatgccgt ctgaagcctt
      361 tcagacggca tttggacgtt taaaacttca tttccaagct aaatgtgtag tttcggccgg
      421 gggcggcata tcggttgtaa acgccgacat ttttgtgttg gttgactgcg ccgccggcag
      481 tttgccgcac attttcccaa gtaacatagc ggtagttgag gaggttgtac acgcccgcac
      541 ggagggtgaa gtgtttttta atcgtgtaat aaccggacac atccacaata taccaagggc
      601 gggtacggcg cgcggtggct tttgtattgc ggctgttgcc gttgagcaaa gcacggctgc
      661 ccaacaactc tgtgatttcc ttggctttgg aataagtcag cataccgttc acaccccatt
      721 tgccttccgg ttggtcatag cccaagccga cgacatagcg cgagggttgg atggcatcaa
      781 acaggtgtga ttgaatatcg gtgcggtctg cgcgtttttt gatgtcgcgg acatggacac
      841 gattataggc aaatgtagaa taccaacctt cgggcaattt atcccatacg ccgttccaat
      901 cgattttgcc caaaacattg atgccggtaa tccgcgcgct ttgggcattg aggtaagccg
      961 ggtcgccttt ggctgtttct ttgccgtttt gaatttgcgc ttcataaccc cggacaatca
     1021 aatcgcggta ggcattgttg aaccaacttg cctccaagtt gccgaaatcg cctttaaaca
     1081 cgatgccggc ttctttgttg aacgattttt ccggatcgat tttgaccgcc ttgctttgaa
     1141 cacccgcccg ccagccgtac atttccgcaa acgagggcag gcggaagccg gttgaagtgc
     1201 ggtaagtcaa atccagccag tcggcaggtt tgaggacgat gcctgcgttc caggacaggg
     1261 tgcggtgcgt gccggtggaa acgctgctgt cgtccgaatg cgtgctgcgg tagtcgtagc
     1321 gcaaccccgc gccgacatcc gcccacctgc ccaaacggac attgtcccga actgccgcgt
     1381 aatagctttt gccgttgatg ctgcgcggcg tgcagtccgt ataagtattg ttgcccaagc
     1441 ggcagatttg ctccgtaacc acatttcccc cgcctatgct gacccaatag ggtttgctct
     1501 tgtcgccgtt ggggctgttt tgattgccgt ttttaggggg cgttttcaac gaataggcgc
     1561 gtttggcgtt ttgaaaataa taatcctgat ggcggagatt agagccaaag cggtcaaacc
     1621 cgagattcac gctcaggttg tggcggattt tggcggtatc gaaggatttt ttgaatgccg
     1681 cctgcaagag cctgtggctt tccccgtaaa tcacgcggtc ggatttgtaa taggaaaacg
     1741 gcttgtcggc actcggacgg caatttttgt ccgaaccgtc ggcagaacag tgcgtctgct
     1801 gaaaatgatt gtccaaaccg ataccctgcc ggtcataaga gaggcgggca taatccgccc
     1861 aagtgtcttt atcggcattg gtatagacat attccaaacc gtagcggctt ttggtgtgcg
     1921 tctcgtcgta aaacacgcct gtaccgtatt ccgcgcccac cagcgcaccg ttttcgccgt
     1981 tggtaaacag tccgccgtat ttgtggttgc ccgcatattt gccgttgcct cttatcggtc
     2041 cgtattttct attttcatca aaaaccgcct tggtcaggaa tgccggaacc gtcatatcgc
     2101 gcgtgtcgaa agtttgttgc gtgtgttcga gtatgccgcc gatgtagtgc cgtttgtttt
     2161 caaaacgaaa acccgggcgg aacaaccacg atcggctttc gtatgaaagc ggatcggcga
     2221 ggaagcggtt gggacccgtg tagtctcggg tggaaaccgt ttgccgttcg tctttgccga
     2281 caacctcttt tttcggattc gctttacacg tttcgtaatt tctgttttcg cattcttcct
     2341 caacgatgaa atttgcgtac cgattggtat cctcaaccgg caccagcctg ttaaagctct
     2401 gaacgccgcg tccggcatct tcgtgtgcgc ggatttcccc cgcgcgccgc ccggtgtgga
     2461 tcagcaaagc ctccgcaccg ccgatgcgcc ccgccagcgc gatggattgg gtaagccccc
     2521 ggtttttgcc ggaataggcg gttttactct gaatgcccca ctgcctgcct tccccgataa
     2581 catcgtcggc ggttttggtt tgaaatgcga ccgagcccgc caatgcgccg ctgccttgtt
     2641 cgaccgagtt tgagcctttg ctgatttcga cagctttgac gttttcatac tcgatttcat
     2701 tgattgcgcc gctgctgccc gccgtcctcg tcccgcccaa tgccgcctgc gcggtgtagg
     2761 actgtatttg cgccaagccg tccaccgtca aggaaacgcg gtttttgtcc ataccgcgta
     2821 tcgagtagcc agagcttgcg ccgcgcccct gttcgaccac ggcgatgccg gggtcgtaac
     2881 gcgtcaggtc gcggatatcg agtacctgtt ccttgctgag ggtgtcggcg gttttgacca
     2941 atttgcccag accggttact tcgttatcgc ggcgggtttt ctgttttttg gcttttacct
     3001 gtatggtatc caactgtttt tcctgtgctt gtccggcttg cacattttct gcataaacgg
     3061 gcagcgcagt catcagcgac aggcataaaa tatttaatcg gaacaaatgt tgctgttgca
     3121 taatgtttcc ctaatcttcg ctttcagacg gcatcggaag gagcgatgcc gtcgaagcct
     3181 tattcttgat tgttcggcaa ccgtgcttat tgcacaggct gttggcgttt cgcaccgaat
     3241 acgacagttg cactgcttgc tgaatttcca ttgccggatg caactgttgt attttccgtt
     3301 tgtttatcgc ccgaataggc aaaccatccg cccaactctt cggctttagg tccgtaaaaa
     3361 ccgccctgca ccttggcgtt tgtgatatat gccttaggcg tgccggtggt attgctttga
     3421 tcgagatcaa aacctaattc agcagttttt gccgtacctt caaagccgtt gtccttaata
     3481 ttagccgtga tagtaaaggt tggctgagtc ctatcattag cggttaacgt gccggtaatt
     3541 tttttatcgc caaaattcac ggtaaagtcc gccctgttgc cactcgttgc attggaagca
     3601 ttgccgctcc agcttgtgct tgtgttgttg gcaatatgcc cgtaccaaga cccccgataa
     3661 acgatgtctt gttgctcttt tggaatctct ttttcatcgg tgcgctcgcc ttggaggaac
     3721 atactttgtt caacttgttc cgttttagcg tcagcttgac taccgttttc gcctgcctgc
     3781 atcgcggact tgctgttttt acgcgtcaac attccgtatt tcagataatt gaggttggaa
     3841 cagcagactt caacttcata ggtttttgtt cctgcctgcc cgccgttaac gcccgccgca
     3901 cccgaagcag cttgcgcgcc gcccgcgcct gtttgggctt tggtgtcttt tttatcactt
     3961 tccagcgtgt aggttgtttt gtaggtaaag gctgttccgc cgtttgtacc ttgattggct
     4021 tgatttttcc cactttcgga agcctcgggc aggagcggaa tcataatgcc gtcgacaacc
     4081 agttgggcgg cattgctgaa gttgtcgaga tttttgattg ctgtgccgcc gtgcgtcagc
     4141 tcgaccgcat ccaaaaccgt ggtcagctta ccgtttttag acgacgtgcc tgccgcaccg
     4201 tccgatgctg ccgcacctgt gccgcctgaa gccactgcgc catttctcgg tttgtcttgg
     4261 gttttcgcgc tgccgacaac ggcaactttt tgatcgttgc tcaaaaagcg gaaacccaat
     4321 tcctcaccct gcgggccgaa aaagccgccg ctcaaagaag acgagtcgga aacaaaggga
     4381 tgttgtttgg tttcattctc ttgtggtttg tcggtcgcta ttgccttgcc attgaagcgg
     4441 ttgcctgtta cttgagcctc aaggctgtag tattgcgtgg taggtttgtc gttattagta
     4501 ttactttggc tcgcattatt gcgtatcaat ttacccgtca atttcttatt gccgaaatcc
     4561 acttctaaat tcgaggtaaa tccataaccc tcgtgaccat ctttcagcgt ggattcgttt
     4621 ttgttggaat attcttcgcc ctcatcacca gaaaatccgc tgtagctgtc gccttgcttt
     4681 tttgaaggct ggataatatc gttaaatttt tgtcccagtt tcgtatcggt tacaaaatgc
     4741 catacgccct tgtaggtaac ttttccagaa gcaggaagtt gtcgggaagg gtctttaccg
     4801 tgataaaaga tatagccgtc gtcgcctgac ttaaattttc cttcattgaa ttttctttca
     4861 ctcgccgcat gtttataaaa ccaaccggaa taaacatatt tgaaattgtt gtaacctgtt
     4921 gcttcgtttt ttggttggtt tgcaccgcca ttgatactgc cgttgtgaga attatggctt
     4981 gattgcgtga gataagggga aatgtaaata ttgttgtcgc cattatctgt ttgtacgttt
     5041 gaaatgacgg attcttgttt taatggcagt ttcttgggtt ctgtcggcaa tcctgtcgcc
     5101 tcccaatcat cattctttaa tttaacctca ttttctttag gcattgccgt aggatgccga
     5161 ttcctccgtt tcaacctcat cgcaaaaccg tatccgcctt ggtctttttg ggcttgcggt
     5221 ttttcggaag aaacatcttg atactttggc gcgggacgcg gggcttcggt atcgacagaa
     5281 tcaagatcga agctgccgcc gccgcccaaa caagcactca acaaaaacac aggcagcacc
     5341 atagcagcct gatttaccaa tggattgttc atataaaccc aattcaatta aagaatgata
     5401 aggattatta ttttatttat ttttaacaaa tttgcaaata cttttttatt tttttaggga
     5461 atacaccaaa atcccgtcat tcccgcgcag gcgggaatcc gaacgcgtcc gcacggaaac
     5521 ctatatcccg tcattcccac gtatatcccg tcattcccgc gcaggcggga atc
//
LOCUS       DAWFIT010000115         5409 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFIT010000115 DAWFIT010000000
VERSION     DAWFIT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.115"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..1761
                     /locus_tag="WGQ85_001974"
     CDS             <1..1761
                     /locus_tag="WGQ85_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0751321.1"
                     /translation="LQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKS
                     SIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIPQGNLKT
                     QIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTEAGAAIIALAVTVVT
                     SGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLV
                     VAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEAN
                     ILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEI
                     VGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVEN
                     NAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLDWND
                     AKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQL
                     AQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNM
                     DGSLNQMKTGAAKGRKLNLK"
     gene            1770..2147
                     /locus_tag="WGQ85_001975"
     CDS             1770..2147
                     /locus_tag="WGQ85_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751322.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            2425..2799
                     /locus_tag="WGQ85_001976"
     CDS             2425..2799
                     /locus_tag="WGQ85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751323.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2834..4429
                     /locus_tag="WGQ85_001977"
     CDS             2834..4429
                     /locus_tag="WGQ85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0751324.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGDVGKTLKELGRSRTVKKLVV
                     AAAIAGVSNKIGASSLATWSETPWVNNLNVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIV
                     GEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNN
                     ALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISR
                     GGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIK
                     HVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTK
                     GETKVRIVVQKGTNKIISAYPQK"
     gene            4448..4711
                     /locus_tag="WGQ85_001978"
     CDS             4448..4711
                     /locus_tag="WGQ85_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751325.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            4818..5409
                     /locus_tag="WGQ85_001979"
                     /pseudo
     CDS             4818..5409
                     /locus_tag="WGQ85_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
BASE COUNT         1719 a         1084 c         1305 g         1301 t
ORIGIN      
        1 ctgcaatcag gttgggatac caaactgcaa ggcacacagt ttgaaaccac actgggtggc
       61 gcaaccatac gcgcaggcgt aggcgagcag gcacgggcag atgccaagat tatcctcgaa
      121 gggatcaaaa gcagcatcca cacagaaacc gtgagcagca gcaaatctac tctatggcaa
      181 aaacaggcag gacggggcag taacatcgaa accttgcaat tgccgagttt caccggtccc
      241 gttgcgcccg tactgtccgc acccggcggt tacattgtcg atattcccca aggcaatctg
      301 aaaacccaaa tcgaaaccct caccaaacag cccgagtatg cttatttgaa acaacttcaa
      361 gttgcgaaaa acatcaactg gaatcaggtg cagcttgctt acgataaatg ggactacaaa
      421 caggagggct taaccgaagc aggtgcggcg attatcgcac tggccgttac cgtggtcacc
      481 tcaggcgcag gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat
      541 gcagcattcg cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcaat
      601 atcggtaaca ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc
      661 gccgctaccg caggcgtagc cgacaaaatc ggcgcttcgg cattgaacaa tgtcagcgat
      721 aagcagtgga tcaacaacct gaccgtcaac ctggccaatg cgggcagtgc cgcactgatt
      781 aataccgctg tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct
      841 ttggtgaata ctgcgcatgg agaggcagca agtaaaatca aacaattgga tcagcactac
      901 atagtccaca agattgccca tgccatagcg ggctgtgcgg cagcggcggc gaataagggc
      961 aagtgtcagg atggtgcgat aggcgctgca gtcggtgaga ttgttggtga ggctttggtt
     1021 aaaaataccg attttagcga tatgaccccg gaacaattag atctggaagt taagaaaatt
     1081 accgcctatg ccaaacttgc ggcaggtaca gttgcaggcg taacgggagg agatgtcaat
     1141 actgctgcac aaaccgcaca aaacgcggta gaaaataatg cggttaaagc tgttgtaact
     1201 gctgcaaaag tggtttataa ggtagccaga aaaggattaa aaaacgggaa aatcaacgtt
     1261 agagatttaa aacagacgtt gaaagacgaa ggttataatt tagccgacaa cctgaccacc
     1321 ttattcgacg aaacattgga ttggaacgat gccaaagccg ttattgatat tgtcgtcgga
     1381 acagagctga atcgcgctaa taaaggggaa gcggcacaaa aggtcaagga agttttagaa
     1441 aaaaatcgtc cttatatccc taataaaggt gctgtaccga atatgagtac atacatgaaa
     1501 aataatcctt ttggaaaaca gctggctcaa atttcagaaa agacaacgct tccgacgcag
     1561 caagggcagt ctgtcttctt ggtaaaaaga aaccaagggt tattaaaaac cggtgatagg
     1621 ttttatttag atggccaaca taaaaatcat ttagaggttt ttgataaaaa tgggaacttt
     1681 aagtttgttc taaatatgga tggttcgctt aaccaaatga aaactggggc agcaaaaggt
     1741 cgtaaattaa acttaaaata ggaaatttta tggaaacatt gaatgatata aaaaaaatct
     1801 tgattaatgt ggggctttat caagggtttg atttgacaga tccaaaagta tcagaagaag
     1861 ttaatcatga aacagctaat atgaaatgga ttaaagatta tacttcagac gggaattggg
     1921 ataatgaatt taaggaggat ttaaaaaact ttttagatta tatggaagta tgccaattag
     1981 ccctaaacga taaaaatttc aaaattgcca gtaattcttt atttatggct atgatttacg
     2041 caggtaatct atctcttata tttgattcaa taaaaactga tatatcaaca ttattgagtg
     2101 ctgagtataa aaagaatagt ttttcatggc catctcttga tgaatagaaa gcaagttgta
     2161 gcctgcatga aatctaaaac ccatgcataa ggtgtgggct tcagtatacg cgttccatga
     2221 tttacggcca tatgccgtct gaaaagctca attttttcag acggcatttg ttatgaaagt
     2281 aaatatttag attccctgta tactgtttag actcgtgtgt gctgagtaag ctgtagtctg
     2341 catgaaacct aaaactcgct caaaattaag ctaagacatt agcagggcaa gggcgaaaat
     2401 tgaatcttaa ataaggtgat tcagatgaaa aattttaatg tagtaaaaga aagtttaaga
     2461 gagttaggaa ttaaacaagg atttgatctt tatgagaaag ccacaactga aaaattgaat
     2521 agtgaagatc ctcttgactt acaatggctt tctaactatt catctgattg gaatgatgaa
     2581 ttagaagaag actttgattc tttttttcag catatgaagg aatatcaata tgctattgac
     2641 aatgaagaca ttaaatctgc atgtagttca ctatgtgaag ctatgctcta tgttggtaat
     2701 attaaaaatt tttttgagtt tctcaaaagc gatatgatta gactgttgag aggtgaaagt
     2761 aaaacaacag actttcaatg gccgcaattt gatgaatagc agcaagctgt agcctgcatg
     2821 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcacgc acgcgttcca tgatttacgg
     2881 ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat
     2941 tcagattccc tatatactgc ccagatgcgt gcgtgctgaa gacaccccct acgcttgcta
     3001 tttgaaacag ctccaagtca ccaaagacgt caactggaac caggtacaac tggcgtacga
     3061 caaatgggac tataaacagg aaggcttaac cggagccgga gcagcgatta ttgcgctggc
     3121 tgttaccgtg gttactgcgg gcgcgggagc cggagccgca ctgggcttaa acggcgcggc
     3181 cgcagcggca accgatgccg cattcgcctc gctggccagc caggcttccg tatcgctcat
     3241 caacaataaa ggcgatgtcg gtaaaaccct gaaggagctg ggcagaagcc gcacagtgaa
     3301 aaaactggtt gtagcagcgg ccatcgcagg cgtatccaac aaaatcggtg cttcctccct
     3361 tgccacttgg agcgaaaccc cttgggtaaa caacctcaac gtcaacttgg ctaatgcggg
     3421 cagtgccgcg ctgattaaca ccgccgttaa cggcggcagt ctgaaagaca atctggaagc
     3481 gaatatcctt gcggctttgg tgaatactgc gcatggagag gcagcaagta aaatcaaaca
     3541 attggatcag cactacattg cccataagat tgcccatgcc atagcgggtt gtgcggcagc
     3601 ggcggcgaat aagggtaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     3661 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     3721 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     3781 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     3841 agatattatt tgggatatcg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     3901 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     3961 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     4021 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     4081 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     4141 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     4201 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     4261 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     4321 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     4381 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     4441 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     4501 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     4561 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     4621 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     4681 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     4741 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     4801 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcacaag cacgagttgg
     4861 atgtccaaaa aagccgccgc tttatcggca tcaaggtagg taagagcaat tacagtaaaa
     4921 acgaactgaa cgaaaccaaa ttgcctgtcc gcgtcgtcgc ccaaactgca gccacccgtt
     4981 caggctggga taccgtgctc gaaggtaccg aattcaaaac cacgctggcc ggtgccgaca
     5041 ttcaggcagg tgtaggcgaa aaagcccgtg tcgatgcgaa aattatcctc aaaggcattg
     5101 tgaaccgtat ccagtcggaa gaaaaattag aaaccaactc aaccgtatgg cagaaacagg
     5161 ccggacgcgg cagcactatc gaaacgctaa aactgcccag cttcgaaagc cctactccgc
     5221 ccaaattgtc cgcacccggc ggctatatcg tcgacattcc gaaaggcaat ctgaaaaccg
     5281 aaatcgaaaa gctgtccaaa cagcccgagt atgcctatct gaaacagctc caagtagcga
     5341 aaaacatcaa ctggaatcag gtgcagcttg cttacgacag atgggactac aaacaggagg
     5401 gcttaaccg
//
LOCUS       DAWFIT010000116         5385 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFIT010000116 DAWFIT010000000
VERSION     DAWFIT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.116"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(1..486)
                     /locus_tag="WGQ85_001980"
                     /pseudo
     CDS             complement(1..486)
                     /locus_tag="WGQ85_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(572..787)
                     /locus_tag="WGQ85_001981"
     CDS             complement(572..787)
                     /locus_tag="WGQ85_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751326.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(1075..1428)
                     /locus_tag="WGQ85_001982"
     CDS             complement(1075..1428)
                     /locus_tag="WGQ85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751327.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1664..3091
                     /locus_tag="WGQ85_001983"
     CDS             1664..3091
                     /locus_tag="WGQ85_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0751328.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3160..4563
                     /locus_tag="WGQ85_001984"
     CDS             3160..4563
                     /locus_tag="WGQ85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0751329.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYEAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4727..5320)
                     /locus_tag="WGQ85_001985"
     CDS             complement(4727..5320)
                     /locus_tag="WGQ85_001985"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0751330.1"
                     /translation="MYGSHHLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQPAEFSD
                     YQRVYLDETGFDRYLFRPYALSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSP
                     ELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1371 a         1320 c         1380 g         1314 t
ORIGIN      
        1 atggtattgt tttgtccgcc aaaatggacg cgaccgtata gggcgtcttc cgaacccatc
       61 aaccaacagc gctcatagaa acgaccgccc gagcctttgg attctttgta gataccgaaa
      121 ccgaaaactt cttcggcgag catggacgcg gcgcgaataa aatcttcgtc ttccaaaaga
      181 cttacacgaa cgccgtattt atcgaaaaag gttttttcat gaaatgaaaa gctaatttga
      241 tcaatgaaag ccgaatctga tacaccgcgc cgaagaggaa cgcctaacag gtttccttta
      301 ccgtccgtta tgtacgtttc gtaacattcg aagacttcct gaaccctgcc tgccgtttcg
      361 gtttctgtac ccccccctgt tagataaggg gggaaggttt gaagcggttg ccggcttcct
      421 gccgtccgct ggcgcgtccg tcatcacgcc gacaaccgcc tttgtcatcc cttgcttatc
      481 ttccatggtg cgaatcctca aaaacgggca aaaaaaaagc cctgttactt gtagaaagta
      541 aaggacgtta atttttgtta atcgtccctt cttagggacg caatatataa ggttttaaaa
      601 cgctaagaac aaaatccgat aacggctctg aagaagtaag tctttcttta ttgcacaaga
      661 tagcaaactt ccacggcatt ctcgccccac ccgtgccgtc caagcctttt tcaaagacat
      721 agatggtttt cttggcaatc acttcgtcag tttggcggtc gataatccgt atggtcgcac
      781 ctgccaccca gtgcctgcgc agcttggaat cgacattgtt ttcgaacgtt accgcataac
      841 gggcgaggtg tatatgatta agtatttgat ttatcggaaa atctttacct gaataccgaa
      901 taatatcgga atggttgggt tgcaggacat ccacatagcg gtattcccca tcgctaaaaa
      961 atcctaggaa acgagcaata aaatttactc cttcgctggt ctgtaaagcc gcattgtccc
     1021 acatcgggtc tctggttttt gcatctgccg aaacggtacg ctcaggtact accttcaaca
     1081 gcataatccc ttccacattg tccgccgtct ggtaaatctt tcccccgccg ttttgcattg
     1141 ttcgttaaac acggcttcgg cttctttgta ttttctgtcc cactcttctt gtgcctgtat
     1201 ttcttctttg atcgggccga attgtttggg aataatccaa acaaacagca tcaggatagc
     1261 ggcggcggtc aggctgcctg aaaggatttt gccggggttc cgtttgggct ttttataggc
     1321 aaagcggacg agaaaccaaa gcaacagcag catggtgccc caatagccga ttgagaatag
     1381 gatggccaaa ccttctagga aatggcgtaa atcgtttgtg gtaaacatgg gttgttcctg
     1441 tggttaaatg tgcaggctgc tttttgccga atcttgccgc atctcaaaag cagcctgcgc
     1501 ttcagcgttg cgttacgcag taaaataatg aatatttgta acggcttggg tattttttgt
     1561 caatattccc gcctttccct taacagctgc cgcgctttcc gttaaaattc ctttacatat
     1621 ttatattgtt tcctgtttct atattgccaa ggttatactc gttatgtttt tctccgccct
     1681 gaaatccttt ctttcccgct acattaccgt atggcgcaat gtttgggcgg tgcgcgacca
     1741 gttggaaccg cccaaacgca cggcggaaga acaggcgttt ttgcccgcgc atttggaact
     1801 gaccgatacg ccggtctctg ccgctccgaa atgggcggcg cgttttatta tggcgtttgc
     1861 gcttttggct ttgttgtggt cctggttcgg caaaatcgat attgtggcgg cggcttcggg
     1921 caaaacggtg tcgggcgggc gcagcaaaac catccagccg ctggaaacgg cggtggttaa
     1981 ggcggtacat gtgcgcgacg ggcagcatgt gaaacaggga gaaacgctgg cggaactgga
     2041 ggctgtggga acagacagcg atgtggtgca gtcggagcag gctttgcagg ctgcccaatt
     2101 gtccaaactg cgttatgaag cggtattggc ggcattggaa agccgtaccg tgccgcatat
     2161 cgatatggcg caagcacggt ctttaggtct ctccgatgcc gatgtgcaat cggcgcaggt
     2221 gttggcgcag caccagtatc aggcatgggc ggcgcaggat gcgcaattgc agtcggcttt
     2281 gcgcggccat caggcggaat tgcagtcggc caaggcgcag gagcagaagc tggtttcggt
     2341 gggggcgatc gagcagcaga aaacagcaga ctaccgccgt ttgcgggccg acaattttat
     2401 ttcggaacat gcgtttttgg agcagcagag caaatcggtc agcaattgga acgatttgga
     2461 aagtacgcgc ggtcagatga ggcagattca ggcggccatt gcacaggcgg agcagaatcg
     2521 ggtgctgaat acgcagaacc tgaaacgcga tacgctggat gcgctgcgcc aggcaaacga
     2581 acagattgac caataccgcg gccaaacgga taaggcaaag cagcggcagc agctgatgac
     2641 aatacagtcg cctgcggacg gcacggtgca ggaattggcc acctatacgg tgggcggtgt
     2701 ggtgcaggct gcccaaaaaa tgatggtgat tgcgcccgat gacgacaaaa tggacgtgga
     2761 agttttggta ttgaacaaag acatcggttt tgtggaacag ggacaggatg cggtggtgaa
     2821 gattgagagt tttccctata cgcgctacgg ttatctgacg ggcaaggtga aaagtgtcag
     2881 ccatgatgcg gtaagccacg aacagttggg cttggtttat acggcggtgg tgtcgctgga
     2941 caaacatacc ttgaatattg acggcaaagc agtgaatctg acggcgggca tgaatgtcac
     3001 ggcggagatt aaaacgggta aacggcgggt gctggattat ctgttaagcc cgctgcaaac
     3061 caaattggac gaaagcttta gggagcgata ggcggatccg tactgggcat ttgttatccg
     3121 ccggttcgga catgcagact gcctgaaacc attgcccgga tgacattgct caatctaatg
     3181 ataatgcaag attacggtat ttccgtttgc ctgacactga cgccctattt gcaacatgaa
     3241 ctattttcgg ctatgaaatc ctatttttcc aaatatatcc tacccgtttc actttttacc
     3301 ttgccactat ccctttcccc atccgtttcg gcttttacgc tgcctgaagc atggcgggcg
     3361 gcgcagcaac attcggctga ttttcaagcg tcccattacc agcgtgatgc agtgcgcgca
     3421 cggcaacaac aagccaaggc cgcattcctt ccccatgtat ccgccaatgc cagctaccag
     3481 cgccagccgc catcgatttc ttccacccgc gaaacacagg gatggagcgt gcaggtggga
     3541 caaaccttat ttgacgctgc caaatttgca caataccgcc aaagcaggtt cgatacgcag
     3601 gctgcagaac agcgtttcga tgcggcacgc gaagaattgc tgttgaaagt tgccgaaagt
     3661 tatttcaacg ttttactcag ccgagacacc gttgccgccc atgcggcgga aaaagaggct
     3721 tatgcccagc aggtaaggca ggcgcaggct ttattcaata aaggtgctgc caccgcgctg
     3781 gatattcacg aagccaaagc cggttacgac aatgccctgg cccaagaaat cgccgtattg
     3841 gctgagaaac aaacctatga aaaccagttg aacgactaca ccgacctgga tagcaaacaa
     3901 atcgaggcca tagataccgc caacctgttg gcacgctatc tgcccaagct ggaacgttac
     3961 agtctggatg aatggcagcg cattgcctta tccaacaatc atgaataccg gatgcagcag
     4021 cttgccctgc aaagcagcgg acaggcgctt cgggcagcac agaacagccg ctatcccacc
     4081 gtttctgccc atgtcggcta tcagaataac ctctacactt catctgcgca gaataatgac
     4141 taccactatc ggggcaaagg gatgagcgtc ggcgtacagt tgaatttgcc gctttatacc
     4201 ggcggagaat tgtcgggcaa aatccatgaa gccgaagcgc aatacgaggc cgccgaagca
     4261 cagctgaccg caaccgagcg gcacatcaaa ctcgccgtac gccaggctta taccgaaagc
     4321 ggtgcggcgc gttaccaaat catggcgcaa gaacgggttt tggaaagcag ccgtttgaaa
     4381 ctgaaatcga ccgaaaccgg ccaacaatac ggcatccgca accggctgga agtaatacgg
     4441 gcgcggcagg aagtcgccca agcagaacag aaactggctc aagcacggta taaattcatg
     4501 ctggcttatt tgcgcttggt gaaagagagc gggttagggt tggaaacggt atttgcggaa
     4561 taaagcaggc tgaaacggtt atgaaattcc caaagcagcc tgcaccccgt ttcgaaagtg
     4621 caggctgctt tgggattgat ccgatacttt cacattctca ttgcctttaa ataaagcaca
     4681 gttaaaaaca catttaaatt aagtgcgaat taattcaaat gtatattcaa ttaaaatcaa
     4741 aataggacag tagtgcatcg tcaaatcggg cgtaatcaga caaaacggtt cgcagatacc
     4801 gcttaatatt cgcccacact ttctcaatcg ggttaagctc aggtgaataa ggtgcaagag
     4861 gcaatacctt atgtcccaat ttttccgcca tttcccgtaa gacacccata cggtgaaatc
     4921 gcgcattatc taaaataatc accgattttt gagtcaatgc gggcagtagg cattgctgaa
     4981 accacgcttc aaaaaagact ccggtcatcg tattttgata aaccatcgga gcaatcagcc
     5041 ggttgccgac ttgtgcggac accaaagata agcgttggta tctttttcca cttatctgcg
     5101 ctttcactat ttgccctttc aggctgaggg catagggacg gaacaggtag cggtcaaatc
     5161 ctgtttcatc caaataaaca cgttggtagt cggaaaattc ggccggctgt gtcaaataat
     5221 gcgttacttt ggccgggtct tgttctttgt aagtggtggt ctttttttgc gcgttatccc
     5281 catctgtttg agtgcatagc aaatggtggc tgccgtacaa tcaaaatgtt tggcgatttc
     5341 atgcagatag gcatccgggt gttgcccaac atattgagcc ggttt
//
LOCUS       DAWFIT010000117         5348 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFIT010000117 DAWFIT010000000
VERSION     DAWFIT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.117"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(160..2994)
                     /gene="lbpA"
                     /locus_tag="WGQ85_001986"
                     /gene_synonym="tbpA"
     CDS             complement(160..2994)
                     /gene="lbpA"
                     /locus_tag="WGQ85_001986"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0751331.1"
                     /translation="MNRRHTFPLTLTALAIAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDILSPNKKAEYFLAEGESEL
                     KPVAKLAGNGNYLKNQLNRWVEERRSKGQALNSEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SDKSGVYDGNDFRDGLYFRPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEST
                     GFDDDNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSAKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2991..5132)
                     /locus_tag="WGQ85_001987"
     CDS             complement(2991..5132)
                     /locus_tag="WGQ85_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0751332.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNG
                     PYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKQKTDAANASDTKPALTSGKHTKILDSLKISVDEAADE
                     KARPFEVSTMPDFGHPDKLLVEGREISLLKDTQTIVLADGRTMTVSACCDFLTYVKLG
                     RIKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDEDE
                     EEEPEETEETEETEETEETEETEETEEKSPTEEGNGGSGSILPTPEASKGRDIDLFLK
                     GIRTAEADIPQIGKARYTGTWEARIGEPIQWDNKADKAAKAEFNVDFGEKSISGTLTE
                     ENGVEPAFHIENGKIEGNGFYATARTRDNGINLSGTGSTDPKTFKANDLRVEGGFYGP
                     KAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1125 a         1456 c         1261 g         1506 t
ORIGIN      
        1 ccgataaatt gccttagcat tgaatgtctg gattcccgcc tgcgcgggaa tgacggcgga
       61 aatcttgttt atattgaatc aaaaaaacct gcaccttaat cagttggcgg tttagtccga
      121 cttttggggt gcagatcaag ctttcagacg gtatttcctt taaaacttca tttcgagcgc
      181 gaggctgaag tttctgcccg gtgcggcata ccttccatag ttactgtcgc cgccgtgccg
      241 gtttgccgtg ctttccgcag tctggcgcaa ggattcccaa gtaacgtagc ggtagttgcc
      301 gatattgtag atagccgccc tcaaggtcag ccgttttttc agattcagat aggcggaaac
      361 gtctgccgtc gaccaagaag acgacgctct ttttgccgaa tatcgttttt gatcgcctgc
      421 cagataagca agctcgtcag ggtttttccc tttggaatag gtcagcataa tgtttgcgcc
      481 ccatttcccc tcgggctggt cgtatccgaa ccccaaaaca taacgcgacg gctgtaccgc
      541 atccaaagca tagctgcgga gggacagtcc cggccggttg gataccgatt tcggtttgat
      601 gcggttgtac gccaaagtcg tgtacaaacc ttcgggcagt ttgccgtaca caccgttcca
      661 gtcgattttt cccaatatat taacgccttg aagcgacata ttttgggcat tgtaataatc
      721 gcgtatatca atctctgtca attgtcctgc ctgattcggc aatttggttt tgtgatcggc
      781 aacggcaatc atatcggtat agcggttgcg gaagctgctg atttccaaaa agccgaagtc
      841 gcctttccac tgcaaaccga tttcccggtt ggctgccttt tccgatttca gggcgggacg
      901 ctgccagcct ttcggataat cgtgataaat gtctatcccg aaaagttctt ggaatgaggg
      961 cgttctgaag ccgctggagg cgcggtaaga cacggaaaaa tgccggttcg gtttgaacac
     1021 gatgccgctg ttccacgaac ggtcaacata ccgtccgctg cggacgagtt cttccgacgt
     1081 ggtgaagttt ttccggtcat acctgccgcc caagctgaaa tcgaaatatt tgccgattga
     1141 aaaacggtcg ttcaaagaaa tgtggatatt gctgccgttg atttttcttg gcacgcattt
     1201 gcgggaacgc agggtttcga tgtagccgca gaccgaccct tcgacgactt cgggcttacc
     1261 caaacggtac ttatcttgat tgtcatcatc gaatcccgtg gattccgaaa tccttgccgc
     1321 attgtgggaa agctgttcgg ggcgggagat cgctttggaa gcatcgtaac cgaagcccaa
     1381 agtcagatgg tgtttcgtcc atttgttttt cagcgatttc tcaaacgagg cattcaaaac
     1441 attgtgctgt tcgcggtagt ggaaacggtc gctgctgtcg taggaatacg gtttgtccgc
     1501 cgacgcgcgg caggatttgt ccacagcagg atacacggcg caattcagct tcagcgtgtt
     1561 gttgtcggtt gccacgccct gtttgtcaaa cgacaacacc gccttatccg cccagttgtc
     1621 agaatacgct tcgttttcat aacggtacag caaacccata cggcggcggc ggtgatgttc
     1681 gtctataaat ttggtgcggg aatatttcaa acctatgccc ttgaccaaat ttgtatcgcc
     1741 cttccactct tctatattcg gcctaaaata caagccgtcg cggaaatcgt tgccgtcgta
     1801 caccccgctc ttgtcgctaa actttttcgc ctcatccgta ccgtaatact gtttttccgt
     1861 catatcgcgg atgtcgtaac gctgtttggt atcctcaaac acgccgccga cataatgcct
     1921 gccgccgaag cggtagccca gcttggcaag ccacgaaccg ctgcggtaat ccatcggatc
     1981 gggcaatatc ctgccgccgc ccgtgtaagc ttgggcggac aggttttcgt ggcgcgcctg
     2041 cgcctcccgc accatcgctt cttcttcact gtttaaagcc tgaccttttg aacggcgttc
     2101 ttccacccag cggttgagct ggtttttcaa atagtttccg ttgcccgcca attttgccac
     2161 aggcttgagc tcactctcgc cctccgccag gaaatattcc gcctttttat tcggactcag
     2221 aatatcgtat gtctggcgga acgcgtccaa acggtttatg ccgtatgcca ccccgtccgc
     2281 aatatcgccg tgcgggcgcg tttcccgccc ttggcgttcg gttcggatta gcagcccttc
     2341 ccaaccgtct ttgctgaacc ccgcgccgag cgacttcata aattggcggt ttttactgcc
     2401 gtaggcagtt tttgcctgta tcccccaact tttgccgtct gaaatcaggt ctgccgcttc
     2461 tttggtgcgg aaggcgaccg cgccgccgag tgcgccgctg ccgtgatcgg acgaaccggc
     2521 gcctttgtcg atttccaccg tgctgatgtt ttcatattcg atttcgttga ttgcaccgct
     2581 gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg gtaaacgcct gtatttgggc
     2641 aacgccgtca accgaaaccg ccacacggtt tttatccacg ccgcgtatcg agtagccgcc
     2701 gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga tcgtagcgcg tcaggtcgcg
     2761 gataccgagt acctgttctt tgttcaacgt ttccgacgtt ttgacgattt tgcccaaacc
     2821 ggtcgcctct ttcgatcgcc gtcccacttt ggcggcacgg acggtaacct ctttcaagga
     2881 ttgggtctgc gccgcatcag gtgccgccgt ttcagggttt gccgcatatg acgggaaggc
     2941 ggcggcaatg gccaaggcgg tcagggtaag cgggaatgta tgccttctgt tcatcgtgtt
     3001 gcctccttgt catctttctt cgcaccgaat accgcaccga atttccggtc gctgtcgata
     3061 atattaccgc ccaattccgc cgccttcgga ccgtaaaatc ctccttctac acgaagatca
     3121 ttagctttga aggttttggg gtcggtcgaa ccagttcccg aaagattgat gccgttatcc
     3181 cgagtgcgcg ctgtcgcgta gaaaccgttg ccctcaatct tgccgttttc aatatggaaa
     3241 gcaggttcta caccgttttc ctccgtcagc gttccggaaa tcgatttctc gccgaaatca
     3301 acattaaact ctgcttttgc cgctttatcc gccttattgt cccattgaat gggttcgccg
     3361 atacgcgctt cccaagtgcc ggtatagcgt gcttttccaa tttgcggaat gtcggcttcc
     3421 gccgtgcgga tacctttcag gaaaaggtcg atgtccctgc ctttagaggc ttccggagtg
     3481 ggcaggatgc tgcctgaacc gccgttgcct tcttctgtcg gcgatttttc ttcagtttct
     3541 tcagtttctt cagtttcttc agtttcttca gtttcttcag tttcttcagt ttcttcgggt
     3601 tcttcctctt cgtcttcatc agtttcttct tcggtttctt cagcttcctc ttcttcggtg
     3661 acttcatctt cgccgttatc atcttcggaa atttcgtctt cgctttcttc gccattatca
     3721 atgtccgaat cctcttcatc ctgcgccttc ggtttgacgg cggggcgttc ggtttttatc
     3781 cgtccgagtt tcacataggt cagaaaatcg caacaagcac tgacggtcat tgtcctaccg
     3841 tcggcaagaa cgatggtttg agtatctttg agcaaggaga tttcacgccc ttcgacaaga
     3901 agtttgtcgg gatgaccaaa atcgggcata gtggaaacct caaacggacg ggctttttca
     3961 tcagccgcct cgtcaacgga aatttttaaa gaatccaaga ttttggtgtg ttttccagac
     4021 gtcagggcag gttttgtatc tgatgcgttt gctgcgtctg ttttttgttt gcctgcgaat
     4081 acgccgaata cgctgttgtc gttgctgata aaccgtccgg caagctcttc tccgttatcg
     4141 ccgaaaaaac cgccctcaag ccgctgatcg gcatcgtcat ggaaaaacaa atgctcttta
     4201 tcagcgtggc tcgtcttcaa cttggtgtta actttggcac tgccggtaaa gcggttgccg
     4261 tccaatgttg cagtaatgtc gtaaatggtc agcggattat agggaccatt tctatctcgc
     4321 acatactgat ttttaatcag ctcgcccttt aggatttttt tgtcgaaatc aaccgtatat
     4381 ttggcaggat gcttttccct ttcgtcgaca tccttagccg cataagaagt tgcaccaaca
     4441 tcgttaccat aaaatgtgat ataacccaaa ttgtcaatgc caaccgcctt acctgctcga
     4501 tgacgtttga catcggtcat atattgccag ttaccagaat attccaccgt tcccgcactc
     4561 ggtaaagatt gggaaggacg ttctccggaa taatatacaa aaccgtcata acctaagcgg
     4621 ttggaaaact gcttaccacc tgagttctgt tcgatttcat ctgttccaaa tctagtataa
     4681 acatatccgg cccggacatg ttcatattta tacacagcat cttttaaatc ggacgtgtag
     4741 attgttacat taggataccg tccgttgatt tcctttttaa gtttgtcagc ctgttctttc
     4801 ggcgtaccgt ctaaaaacag gatatcctcc tctttgagcg gcagatactc ctctgcctgc
     4861 ttactatcgg gaatttcatt accattttta tcaaaagctg cgatattccg ccttggcagc
     4921 cgcattgccg caccgacggc gggccggttg accggcgtgg tttctaccga aggcccggca
     4981 gggggcgaag tgggaacgtc cttagacttg aaagtgacgg ggtacgcggt cggcgttgat
     5041 tcgacaacag gctgcacgcc gaaattgccg ccgatgcaag atgccaaaag taagggcaac
     5101 aagacaatgc cgccataatt cggtttacac atccctactt ttcctcctat ttgattaata
     5161 ataattatca ttatattaat atgtacagat aatatcaacc cgtttttata gtgaattaac
     5221 aaaaatccgg acaaggcgac gagccgcaga cagtacagat acatcccgtc attcccacga
     5281 acctacacca cgtcattccc acgaacctac attccgtcat tcccacgaac ctgcaccacg
     5341 tcattccc
//
LOCUS       DAWFIT010000118         5308 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFIT010000118 DAWFIT010000000
VERSION     DAWFIT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.118"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(90..1445)
                     /locus_tag="WGQ85_001988"
     CDS             complement(90..1445)
                     /locus_tag="WGQ85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0751333.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="WGQ85_001989"
     CDS             complement(1460..1732)
                     /locus_tag="WGQ85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0751334.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1858..2595)
                     /locus_tag="WGQ85_001990"
     CDS             complement(1858..2595)
                     /locus_tag="WGQ85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0751335.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2876..3775)
                     /gene="prmB"
                     /locus_tag="WGQ85_001991"
     CDS             complement(2876..3775)
                     /gene="prmB"
                     /locus_tag="WGQ85_001991"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0751336.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3920..4513
                     /locus_tag="WGQ85_001992"
     CDS             3920..4513
                     /locus_tag="WGQ85_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751337.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGTLTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4625..5062
                     /locus_tag="WGQ85_001993"
                     /pseudo
     CDS             4625..5062
                     /locus_tag="WGQ85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         1451 a         1364 c         1182 g         1311 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggtgcggga
      121 atcctgccgc cccggttgac gaacacttcg catgagcctt cttttaccgg cattacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggttttacc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggtgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc ttcggcggcg
      421 gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgcg taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaggc ggttttcttc
      721 acaacttcgg caacttcggt caatgtcgtt gcatccgaat tttccaacgc ggcttttacg
      781 acacccgggc cggatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacgggtt gtcttccacc gcgttgcaga acacgacgat tttggcgcag
      901 ccgaaacctt ctagtgtgat ttcagccgtg cgtttgatgg tttcgcccgc cagtctgacc
      961 gcgtccatat tgataccggc gcgcgtactg ccgatattga tggagctgca cacgatatca
     1021 gtagtcttca tcgcttcggg aatggaacgg attaacacct cgtcagaagg cgacatacct
     1081 ttttgcacca gcgcggaaaa gccgccaata aaagacacgc cgatggcttt ggcagcctta
     1141 tccaaagttt gcgccacgct gacgtaagaa tcagcatggg tggccgccgc gatttgggca
     1201 atcggcgtga cagaaatgcg ctgattcaca atcggcacgc cgtatttggc agacagatat
     1261 tttgctgtcg ccaccaagtc tttgccgacc gtggtaattt tgttgtaaat attttggttc
     1321 aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa tgccgatggt aatggtgcgg
     1381 acatcgaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc ggattggata
     1441 ctcatcacat tcctccggct caaatgcggt gcatcgcttg gaagatttct tcgttttgca
     1501 tacggatgtc gagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcctgcc
     1561 gcgatttgct gcattttgaa gtgtccacca agataatcat ggtaaaaaaa tcatccatca
     1621 gctgttggct gatattgagg atgttgattc ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccaccg ttggattttg aagaaatatt
     1801 gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacagca tcaagattca
     1861 gggttcgatt aaataaccat ccttatccca ctgggttttc ctgaccaact tatcatcctg
     1921 ataaacagct tcactctttt tagaaccatc ttcataccac tctaagacta ctccattacg
     1981 tttatgatga tggatagaca gttccgagag taatcgaccg ctttcatccc atgtcagaat
     2041 tttggcaggc tcatcattga ccataaccat ttccgttttg atattgccgt cggcatacca
     2101 ttgcttccat acgccgtttg ccttattctg tttaaactgg atttcgcttt ccttgccgcc
     2161 gttacggtaa tagcggtacc ccgtaccttc actcaaacca tttttataag gcataacggc
     2221 agatttttta ccgttcggat accagttgac ccactccccg tccggcttac ccttgctgaa
     2281 gccccccgcc atttttttct gaccattaaa atgccacaaa atcaacatac cgttttgcag
     2341 ggtaggcaca aaagatttga tttgcgttga agcaacgata taaggttcgg aatatttctt
     2401 catcgacgga taataaaaat cctgcgcgtg cgcaataccc tccgccacac tatattgcct
     2461 gatataagcg gcagaagaca tcgtcgccgt cagtttcccg ttctgattaa aataaacaga
     2521 ataggtctgc gccggcaaag cggccgaaaa acccaacagg acagttgaaa atacaatccg
     2581 agataatttt ttcattgcaa tagcgatata aaaacaaggc tgtgttttag taatctgttg
     2641 atttcaatta tttgcaaggg aaaagacaat tattttccgg ttaggaataa acctattcta
     2701 ttgaatagat tgaagccaag tacgcctatc aacactatat taaaacactg ccaaaaacaa
     2761 ttaacttata aacaatatgg taaggatttc tctgccaagc atcaaacccg agacaacgta
     2821 tcgtaaaaat gccgtctgaa aacaaatcgt cttcagacgg catttccctt tcaactcact
     2881 cttcacccaa taactgctcg cgcgtcaaga ggaaaacaaa accgtcgccc ccgctggttt
     2941 ccaaccaagt aaaaggcaac tccggatacg ctgcttccaa tacatccctg ttatgcccga
     3001 tttccaccag caatacacct ttgggattca gaaactttgc cgcattcaga agaatctgcc
     3061 tggtggcatc caacccgtcc gccccgctgc ccaatgccaa ttccggttcg tgcaaatact
     3121 cttcaggcaa taactcaacc gattccgcat ccacataagg cggattggaa acaatcaaat
     3181 cataagtgcc ttccaatcct tcaaacaaat ccgtatgaat aagccggatg cgttcttcca
     3241 aaccataatc ttcgacatta atccctgcca cttccaaagc atccaagctc acatcaaccg
     3301 catcaatttg ggcatcagga taatgatgcg ccatctgaat ggcaaggcaa ccgcttccgg
     3361 tgcaaagatc caaagcatta tgcaccaact catcgtattc tatccaaggg cgaagtccgt
     3421 cacccaacaa ttcataaata aaagaacgag gtatgattac gcgctcatcc acatagaaat
     3481 caaactctcc ctgccatgcc tggtgtgtca aataagcggc tggaatgtgt tcgacagcac
     3541 gacgctcaat aaccgccagc acttcctctt tttcagcttc caagagtttt gcatcaagat
     3601 atggggcaag catatccaaa ggcaaattca aagtatgcag aatcaaataa gccgcttcat
     3661 catgcgcatt atctgttcca tgaccaaaaa agagccctgc ctcattaaaa cggctgactg
     3721 caaaacgtaa aatatcgcgg atagtcgtca attcttgtgc tgcctgatta aacataatat
     3781 gaaccattct gcgtatagat acttttaatt ataacagaaa caacaagcaa accttttcat
     3841 atcgccaaat aaccacccaa tctacccata caactacata aatgcccgag cgaaaaccat
     3901 cgcccgaacg gaaacgacaa tggccgacgg tatgggcaat ctgattggct gggaaaaaac
     3961 ggggcttgtt gccggtaagc agtggataac cgcaaaagac gacaaggtgt ccgatgtctg
     4021 caatgccaac ggcgagatgg gcgtaatcgg gctttacgag cctttctcac acggcacatt
     4081 gacgataccc ggtcatccga actgccgatg cgaggttgtt tccgtatcgg gtggcgaatt
     4141 gggggaattt gccgaaaaaa aggagcttcg taaagcggct atgcagtatg cgcgggataa
     4201 ctttatcggc aaaagctatg tcaatgaaaa cagcgggcat gaactgaagg taacttggca
     4261 aggtgtgaaa cacgctacgt caaaggcaaa tcaggcggaa ttatccatca tgacaaaact
     4321 tgatgactta ttgcgctacg caaaatatga gggttcttat tcggatagga aaggtcatcc
     4381 tgatattatt gcagcacata agtatcgtgc cgttgccaag gttgggaatg agtctttaaa
     4441 tatcggtgtg attgtaaggg aatttccaga cggacataaa cattacgacc atttcatctt
     4501 gaaggatgag taaagccctt ttgcagtgtc gttctggagc ggatagcgtt aaggcaagta
     4561 cgcttccagc cttacaaaag ggctttaaat tcagcatgcc atttatacag gcaggagtaa
     4621 acccatgaca aagttatacg cagaaatcgc caagatggag acgcaggacg acggcacggt
     4681 caaggtttgg ggttacgctt caagcgagga aatcgattcg gacggcgaag tcatcgcggc
     4741 ggcagctatg aaggcggcga ttcccgatta tatgaagttt ggcgcggggc gcgagatgca
     4801 cggctcaaac gctgcgggaa cggcaattga aatcaacgtt gaagacgacg gcataacctt
     4861 tttcggtgct catatcatcg accccgttgt cgtgtcgaaa gtcaaaacag gcgtttacaa
     4921 aggcttttcc atcggcggca gcgttaccgc ccgcgatgat ttgaacaagt cgcaaatcac
     4981 gggcttgaag ctgacagaaa tcagccttat cgaccgccct gccaatcccg atgcggtgtt
     5041 tacctgcttt aaggcggaca aaggtgcgga agcggtaaac aacgatacag aacataatgc
     5101 tacatatttt agccatttcc cttccaaaca aaaaacaaaa aagcaccggc ggcagccgat
     5161 gccccttcct ttacaggttc ccctattttt tcactgcggg cagcactggt ttggctgggc
     5221 cttttggtgc gggcgcgccg acggaagcct gatccttcag cttcgccagc accgcagggc
     5281 cgatgccctt taccttggtc aaatcgtc
//
LOCUS       DAWFIT010000119         5033 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFIT010000119 DAWFIT010000000
VERSION     DAWFIT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.119"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..175
                     /locus_tag="WGQ85_001994"
     CDS             <1..175
                     /locus_tag="WGQ85_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0751338.1"
                     /translation="SKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNL
                     LKAANRLSAPAAA"
     gene            complement(594..974)
                     /locus_tag="WGQ85_001995"
     CDS             complement(594..974)
                     /locus_tag="WGQ85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0751339.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1451..3532
                     /locus_tag="WGQ85_001996"
     CDS             1451..3532
                     /locus_tag="WGQ85_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0751340.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3522..3716
                     /locus_tag="WGQ85_001997"
     CDS             3522..3716
                     /locus_tag="WGQ85_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0751341.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(3912..4103)
                     /locus_tag="WGQ85_001998"
     CDS             complement(3912..4103)
                     /locus_tag="WGQ85_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751342.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            4232..4792
                     /locus_tag="WGQ85_001999"
     CDS             4232..4792
                     /locus_tag="WGQ85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0751343.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1194 a         1421 c         1249 g         1169 t
ORIGIN      
        1 gtcgaagacc cgttatgtgg ttgaacagag cttcggtacg ctgcaccgta aattccgcta
       61 cgctcgggca gcctatttcg gactgattaa agtgagtgcg caaagccacc tgaaggcgat
      121 gtgtttgaac ctgttgaaag cggccaacag gttaagtgcg cccgctgccg cctaaaaagc
      181 agcccggatg cctgattatc gggtatccgg gcaggattaa gggggtgttt gggtaaaatt
      241 aggcggtatt tggggcgaaa acagccgaaa acctgtgttt gggtttcggc tgtcgggagg
      301 gaaaggaatt ttgcaaaggt ctcaggccgt ctgaaaaaca gctttgacaa agacgcggtt
      361 gccaaagaga tcgacctaca aagggaagta acgcaggcgt tcggcaaaac gccgcccaag
      421 ccgcagcggc cgttgccgac gaactcggca atacccgaag ttacggacgg tatcaggcag
      481 cccgaaccct gccggaggcc gaactgcaaa acaaatccga cacaaaaaac cgtccgtccg
      541 acacaaaaaa ccgcccgaaa aatctggacg gcggttcaaa caggctgccc cgtttaacgg
      601 gcgcggcagg aagtttcgac cgaattgccg taggcatcgg taaagccgaa aaaggcttcg
      661 ccgcctttct ggtgccactc ggttgcgttt ccgaacaaac cgtgttcggc ggtatagcgt
      721 tcgccggatg cggcaacgtc ggaagagagg acggcacgcc tgccgtccag ccgcaacgcg
      781 actttgccgc tgtccaaatg gcggacgcgc acagacaaac cgttctcgca ggaaaacgcc
      841 cgaaaatcgt ccgtgccggc ttggttttga acgggcggca tatgcccgcg tccgccgtca
      901 tcatacgcct ccggcacggc acaggccgcc aaagacaaaa ccggtacggt cagcgcgaaa
      961 aacctgatat tcataaaagc tccccaataa aaataagata tgaaacaacc gccctgattc
     1021 caagctgcgg caacgccata ctataaacgg acgcgcaaac acacaagccc gataaccgga
     1081 atttacctgc gatgaatcaa taatccggat tgcgcgcccc ttctttaccc ctcttccgat
     1141 gccgcctttt gcctgacgat gccgtctgaa cctgcctgcc cgcccggagg aatgtaaatt
     1201 ttttccaaat tccaagtaaa aaccgctatc ggtgtgctaa tttgcgttaa aatcctattc
     1261 ggcgtttaac gttttgtgcg cccgcatccc tgcactgttt gatgcgggca taaggcacaa
     1321 atcccgacaa gcgcactgtt tcatacttcg tcaatcattc agactccggt ttgtgcccgt
     1381 gccggcagat ggttcggccg tttcccgccg ttcaggcata ttccgacagt gtgagataag
     1441 gatttattcg atgaaatcac tcaaaacctt cctcatttgg ggcatagtgg tactggtcgg
     1501 cttagcatcc tttaccactc tggccctcag ccgaggcgaa caggtcagcg cggtatggat
     1561 ggtcaccgcc gccatatccg tttactgcat cgcctaccgt ttttacagcc tctacatcgc
     1621 caaccgcgta atgcggctcg atcctgaccg cctgactccg gcagaacgcc acaacgacgg
     1681 cttggactac gttccgacgc acaaaggcgt attgttcgga caccactttg ccgcaattgc
     1741 cggcgcgggc cctttggttg gtccggtttt ggcggcgcaa atgggttatc tgcccggtac
     1801 tttgtggatt atcttcggcg tggtatttgc cggcgcggta caggatatga tggtcttgtt
     1861 cgtctctatg cgccgcgacg gtaagtcttt gggcgatatt gtgaaacagg aactcggcac
     1921 tgtccccggc gtgattgcct ccatcggtat tttgatgatt atggtcatca ttatggcggt
     1981 gttggcgttg attgtcgtaa aagcattggt tcacagccct tggggtacgt tcaccattgc
     2041 agcaactatg ccgattgcgc tgtttatggg tatttacacg cgttatatcc gtccgggcaa
     2101 aatcggcgag atttccatcg tcggctttat tttgctgatg ctggcggtaa tttacggcga
     2161 agatgtggct aaaagttcca tcgggcattg gttcgacctt gacggcatcc agctcacttg
     2221 ggcgattatg atttacggct ttgtcgcctc cgtattgccc gtatggttgc tgctcactcc
     2281 gcgcgactat ctctccacct tcctgaaaat cggtacgatt gcggccttgg ctttgggtat
     2341 cgtcatcgtc aatcccgctt tgcaaatgcc tgccgtaacc cactttatcg acggttcggg
     2401 tccggtattc tcaggcgcat tgttcccatt cttgttcatt accatcgcct gcggtgcggt
     2461 ttcgggcttc cacgcgctga tttcttccgg cactacgccg aaaatgctgg aaaacgaaac
     2521 ccacgtccgc atgatcggtt acggcggtat gttgatggaa agtttcgtag ccattatggc
     2581 acttgccgct gccgcatcgc ttgatcccgg cgtgtacttc gccatgaaca gcccagccgc
     2641 cctgatcggt acggatgcca ataccgccgc cgaagtgatt accaccaagc tgcaattccc
     2701 tgtcgatgcc gcaaccctgt tgcacactgc taaagaagtc ggcgaaaaca ccatcctttc
     2761 ccgtgccggc ggtgcgccca ccctcgcagt cggtatggcg cacattatga gccgcctgat
     2821 tccgggcgag gcgatgatgg cgttctggta tcacttcgcc ctgttgtttg aagccttgtt
     2881 catcctgacc gccgtcgatg ccggtacgcg cgtcgcacgt tttatgattc aagacttggg
     2941 cagcatcttc tacaaacctt tcggcaacac cgactccatc cccgccaacc tgattgcgac
     3001 cttcttcgcc gtggcattgt ggggctactt cctctacacc ggcgtaaccg acccgctggg
     3061 cggcatcaac tcgctctggc ctttgttcgg catcgccaac caaatgctgg caggcgtggc
     3121 cttgattatg tgcgccgtgg tgctgattaa gatgaaacgc gaccgttatg tttgggtggt
     3181 actcgttccc gccgtcggcg tactgttcgt aacctgctac gccggcctgc aaaaactgtt
     3241 ccacagcgac ccgcgcatca gcttccttgc ccacgccggc aaatacagcg acgcattggc
     3301 taaaaacgaa gtccttgcgc ctgccaaaga catcggcgaa atggcgcaaa tcatcttcaa
     3361 cgacaagatt aatgccggtc tgaccatcct cttcttgtcg gttgtcgtga ttgtcgccgc
     3421 gtacggtttg cgtaccgccc tcaaagcacg caaagtcggc tggccgaccg ccaaagaaat
     3481 cccggcggtg taccgcgacg gcaagcagcc ggaggcacaa agtgaagcat aagctcgcgt
     3541 cttggtggaa aaccatcaag ctgacggcaa acttgatggc aggcgtgcct gattatgaaa
     3601 actacgttgc acagcagcgc aaacataatc ccaacgcccc cgtgatgacc aagctgcagt
     3661 ttcaagacta ttgccgcaaa cgccgctgcg gcgcaaacgg cggacgctgc tgttagcccc
     3721 gcctgaaaca aatgccgtct gaacgccgct tcagacggaa tttttataat atagtggatt
     3781 aacaaaaatc aggacaaggc gacgaagcca cagacagtac aaatagtacg aaaccgattc
     3841 acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgacgt
     3901 accggttttt gttaatccgc tataccacga tgaatccttc gcaatatctg tttatcgacc
     3961 tcaattttga caaaataccg gatacgcgcc tttgttgctt ttccatcttc caaccaactg
     4021 taaatctcaa acagccggta cacgccatgc ttcagtttct tttcctgtcg gcggattgtt
     4081 tcgacaaaga attgaaaatc catttcatgc accttaaaat ttaatctgca ttcaaacctt
     4141 ttcactttgg aagcaccatt tatcggatgt cccttcgcaa taaacaaatt ttcccgatac
     4201 cgccgcccat ttcaacccaa acccaaaacc tatgaaaaac ctcatcgcct tcaacaaacc
     4261 ctatggcgtt atctgccaat tttcaccgca cgaaaaacac aaaagcctca aagactttat
     4321 caatcttccc ggcttctacc ccgccggacg gctcgacacc gacagcgagg ggctgctgct
     4381 gctgaccgac gacggcaggc ttcaggcaca aattaccgac cccaaattca aacaccctaa
     4441 aacctactgg gcgcaactgg agggcgtacc cgacgaaagc cgattggaaa gcctaagaaa
     4501 agggatagac ttaggcggtt tcgttacccg tccggcaagc atccgcatct tgaaacacgg
     4561 agaagcagat tcgttatggg agcgcatccc gccgatacgc gtccgcaaaa ccgttcccga
     4621 tttttggatt gaaattacca tttctgaggg caaaaaccgc caagtcaggc gaatgaccgc
     4681 caaggcgggc tatccctgcc tgcgtctgat cagagtggca agcggcaggc tgaaactgtt
     4741 tgatttggat ttaaaacccg gggaatgggc atacgccccg tttaaaccat aatcacgttt
     4801 atctcatcat ttccacaaaa gtgggaatcc ggaattttat agtggattaa caaaaatcag
     4861 gacaaggcga cgaagccgca gacagtacaa atagtacgaa accgattcac ttggtgcttc
     4921 agcaccttag agaatcgttc tctttgagcc aaggcgagac aacgccgtac tggtttttgt
     4981 taatccgcta tattccgcca tctctaagat ttacagcgat acacgggtga ttt
//
LOCUS       DAWFIT010000120         4945 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFIT010000120 DAWFIT010000000
VERSION     DAWFIT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.120"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            129..350
                     /locus_tag="WGQ85_002000"
                     /pseudo
     CDS             129..350
                     /locus_tag="WGQ85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(332..640)
                     /locus_tag="WGQ85_002001"
     CDS             complement(332..640)
                     /locus_tag="WGQ85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751344.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
     gene            complement(699..931)
                     /locus_tag="WGQ85_002002"
                     /pseudo
     CDS             complement(699..931)
                     /locus_tag="WGQ85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            867..1094
                     /locus_tag="WGQ85_002003"
     CDS             867..1094
                     /locus_tag="WGQ85_002003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751345.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            1256..2311
                     /gene="aroG"
                     /locus_tag="WGQ85_002004"
     CDS             1256..2311
                     /gene="aroG"
                     /locus_tag="WGQ85_002004"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0751346.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            2379..2867
                     /locus_tag="WGQ85_002005"
     CDS             2379..2867
                     /locus_tag="WGQ85_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0751347.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(3217..3693)
                     /locus_tag="WGQ85_002006"
     CDS             complement(3217..3693)
                     /locus_tag="WGQ85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0751348.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKIMETMCPFKMEKRRYPVRWNGSLFEVD
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(3705..4133)
                     /locus_tag="WGQ85_002007"
     CDS             complement(3705..4133)
                     /locus_tag="WGQ85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0751349.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQNYSNTFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            4245..4490
                     /locus_tag="WGQ85_002008"
     CDS             4245..4490
                     /locus_tag="WGQ85_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751350.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVCKLYSEQKMQKFVWEKRDLIADYISRRMQG"
BASE COUNT         1301 a         1153 c         1259 g         1232 t
ORIGIN      
        1 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
       61 agcaccaagt gaatcggttc cgtactattt gtactatctg cggcttcgtc gccttgtcct
      121 gatttttgtt aatccgctat attttagata atgcgcgatt tcaccgtatg ggtgtcttac
      181 gggaaatggc ggaaaaattg ggacataagg tattgcctct tgcaccttat tcacctgagc
      241 tcaacccgat tgagaaagtg tgggcgaata ttaagcggta tctgcgaacc gttttgtctg
      301 attacgcccg atttgacgat gcactactgt cctactttga ttttaattga ctatagaacg
      361 ttgcggttac gcggaagccg tactcgttgg atttggagcg gcccattttg gttttgtcgc
      421 cgtccaaaac aatctcacgg ggtttgcaga ttgttttgtg acggtagtat ggatcaaact
      481 cgagaccgac gctgtcggtc aactgtttgc ctacattcag accgataccg acactccaac
      541 ctttggcgct tttgctgaca tcgcgggaag cacacatctg ggtcgtcatc actttggttt
      601 tgccgcgcaa atctgcatat gcatccgccc aaggggtcag ggatcatccg tcccccaaat
      661 cttggcggat ttcgccatag actttcaaaa caaggttttc atgcttggta acggtgtttt
      721 tccttatcgc cgatgatggc tttgcccttg ccgttagact caggaatatc ggctaccgta
      781 acggcggaca cggctgcaag tgagagtgca agcagggttt tttcatgttt ttcttcctat
      841 aatgaggatg aataaatgga gaaagtgtgg gaaatacccg cattccaatt aaatcttttt
      901 tcaagcaatt agttcttttt gttttcaaca ttttccttgc gtatgaaatt ttgccggatg
      961 tgcaggcgga atcggcagcg gggtgttctg taccggattg tcgtggaaat gggaaaacgg
     1021 atgttccgtg cggttttgtc cgaatgaatg gcgggtattg tttttatcaa tctgtttctt
     1081 tttctttgga ataaaattcc taaaatatta aaaatatgaa atttaaaatc tatgaaaaaa
     1141 gatatatcag ttattttgaa ataaaatagc tttgtagtaa tatgttgcac ttgtttgtgc
     1201 aaggtaaacg atgtaaccta agccgcgtat aaaaaaccat caggaaagat gcaagatgac
     1261 acaccattac cccacagacg atattaagat taaagaagtt aaagagttgt tgccgccgat
     1321 tgcccatctt tacgagctgc cgatttccaa agaggcttcg ggcttggttc accgcacccg
     1381 tcaggaaatt tccgatttgg ttcacggcag ggacaagcgg ctgttggtta ttatcgggcc
     1441 gtgttcgatt cacgatccga aagcggcgtt ggaatatgcg gagcgtttgt tgaaactccg
     1501 caagcagtat gaaaacgagc ttttgattgt gatgcgcgtt tatttcgaga agccgaggac
     1561 gacggtgggt tggaaaggtt tgattaacga cccgcatttg gacggtacgt ttgacatcaa
     1621 tttcggtttg cgtcaggcgc gcagcctgtt gttgtcgctg aacaatatgg gtatgcctgc
     1681 ctctaccgag tttttggata tgattacgcc gcaatattat gcggacttga tttcttgggg
     1741 ggcaatcggt gcgcggacga ccgaaagcca agttcaccgc gaattggcaa gcgggctgtc
     1801 ctgccccgtc ggctttaaaa acggtacgga cggcaatttg aagattgcca tcgacgcaat
     1861 cggtgcggcg agccattcgc atcatttttt gtctgtaacc aaggccgggc attccgccat
     1921 tgtccatacc ggcggcaatc ccgactgtca tgtcattttg cgcggcggca aagagccgaa
     1981 ttatgatgcg ggacacgtca gcgaggcggc ggaacaactg cgtgcggcag gcgtaaccga
     2041 caagctgatg atagattgca gccacgccaa cagccgcaag gattacactc ggcagatgga
     2101 agtggcacaa gatattgccg cccaattgga acaggacggc ggcaatatca tgggcgtgat
     2161 ggtggaaagc catttggtcg aaggcaggca ggacaagccg gaagtgtacg gaaaaagcat
     2221 taccgatgcg tgtatcggtt gggacgcgac tgaagaactg ttggcattgt tggcaggtgc
     2281 aaacaaaaaa cgtatggcgc gcgccggttg agatttttga cacggaatgt cataaaatgc
     2341 cgtctgaagc gttcagacgg catttttgtg gaggaaatat gctcaaaata accctaattg
     2401 cggcgtgtgc ggaaaacctg tgcatcgggg cgggcaatgc tatgccttgg cacatccccg
     2461 aagatttcgc atttttcaaa gcctatacct tgggcaaacc cgtcattatg gggcggaaaa
     2521 cgtgggaatc cctgcccgtc aaacccctgc ccggacggag gaacatcgtc atcagccggc
     2581 aggcggatta ttgcgcggca ggcgcggaaa cggcggcaag tttggaggcg gcattggcat
     2641 tgtgcgcagg cgcggaagaa gccgtcatta tgggcggcgc gcagatatac ggacaagcga
     2701 tgccattggc gaccgatttg cggataaccg aagtggattt gtctgtggaa ggagatgcat
     2761 ttttccccga aatagaccgg acgcattgga aagaggcagg gcggacggaa cgccgcgtca
     2821 gcagcaaagg cacgagctat gcttttgtgc attatttgag atattgaaat ataaactctc
     2881 tataaaatcc cccgcaaatg atgggctgaa atagaaaata ttgttattcc cccgaagatg
     2941 ggaatccggg attttaaagt tagggtaatt tatccgaaat aacaacaatc ttccatcgtc
     3001 attcccgcaa aagcgggaat ccggaaacga aaagctaaag caatttatcg gaaaaaaccg
     3061 aagtttaaag aaccggattc ccgcctgcgc gggaatgacg agattttagg ttatggggat
     3121 ttattgggaa taatggaaca aagaaagcag aaataaggat atagaggctg tctttggatt
     3181 tgcgatggtt gtcggagaat gccgtctgaa gccgcttcag acggcatttt tccagcttga
     3241 gaacggatgt ttgctcaaat aagcattggt aaacataccg tcggcagtga tttcgcgccc
     3301 cagccagtcc ggacggtcaa aatcggcatt ttcgtcgggc agctcgattt ctgcgacgac
     3361 caaaggtgcg ttttctccaa ggaaaacatc gacttcaaac aggctgccgt tccatctgac
     3421 cggataacgc cgtttttcca tcttaaacgg gcacatcgtt tccattatct tttccgcatc
     3481 ggcaagcggg atttcgtatt caaactcact gcggctgatt tccgaaatat agcctttcag
     3541 cgtcagccac gcccgttttc cggcaatgcg gacacggacg gtgcgttctt tttcaacaga
     3601 cagataaccc tgcctcaaca gcagcggctc atcggcgtat tgccgccagt tgtcgtttcc
     3661 aatcaaaaaa cggcgttcga tttctatcgg cataagatgc tccgtcaaaa cggtttgaac
     3721 acgaccagat acagcgcggc aaccatcagc agcacgggga tttcgttaaa tacgcggtac
     3781 cagcggtgtg agaaagtatt gctgtaattc tgaaaacggc gcagcagcac gccgcaatac
     3841 aactggtaag ccaaaagcat caagcccaaa cacagtttga cgtgtaccca gccgctgccc
     3901 caccagccgg cggcaaacgg tatcgccgcg ccgaacacga ccgcaccgaa acctaaaggg
     3961 gacataaaac ggtacaaccg caccgccata cccgacaggc gcacatactc gggattgccg
     4021 cgcggcacat caatcatcgc catattgacg aaaatcctcg gcaggtaaaa cagccctgca
     4081 aaccacgaaa tgacaaaaaa caagtgaaac agcttgaacc aagaaaacat catcgcccac
     4141 accctgccga aaagcggtat tgtacaggca aaccgcttgg gaaacgtgat aaaatcaggc
     4201 ggataaacaa atcgaataaa tccttaccgc aaaacggagg caaaatgctc aaatccatcg
     4261 aactcaattc ccacatccgc aaccgccttg cagcatatct gaaaggcagg ggtatggatt
     4321 ttcagacggc gatgcgggag gaaaaaggca acaaagaaat cgcctccatc gtccacagcg
     4381 gtctgcccac tctggtctgc aaactgtatt ccgaacaaaa aatgcagaag tttgtttggg
     4441 aaaagcggga tttgattgcc gactacatca gccgccggat gcagggatag gcggctgaaa
     4501 tctgttttca ggcaagtgaa aagacaatat ggcagattga aattatgctt atcgtcattc
     4561 ccgcccgcgc gggaatccga cttgtttggt ttcggttatt tttcgtttcg taacttttga
     4621 gccgtcattc ccgcgcaggc ggtaatccgg cttgttcggt ttcggttctt tttctcgttt
     4681 cgggtgattt ctaaaccgtc attcccgcgc aggcgggaat ctaggtcttt aaacttcggt
     4741 tttttccgat aaatttttgc cgcattaaaa ttctagattc ccgctttcgc gggaatgacg
     4801 gcggagggtt tttagttttc ccgaaaatgc acatcatcca aaatcccgtt attcccacaa
     4861 aacagaaaat caaaaacaac aatctgaaat tccgtccttc ccgcccgcgc gggaatccga
     4921 cttgtccggt ttcggttctt tttct
//
LOCUS       DAWFIT010000121         4840 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFIT010000121 DAWFIT010000000
VERSION     DAWFIT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.121"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..184
                     /locus_tag="WGQ85_002009"
     CDS             <1..184
                     /locus_tag="WGQ85_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751351.1"
                     /translation="DKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRKYS
                     GLTKIRTRRRSRRQYR"
     gene            282..398
                     /locus_tag="WGQ85_002010"
                     /pseudo
     CDS             282..398
                     /locus_tag="WGQ85_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGQ85_002011"
     CDS             complement(431..1540)
                     /gene="mltB"
                     /locus_tag="WGQ85_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0751352.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1537..1719)
                     /locus_tag="WGQ85_002012"
     CDS             complement(1537..1719)
                     /locus_tag="WGQ85_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751353.1"
                     /translation="MPSERGSAFRRHFRRRQADFGRRKRQRAAFCKAADCGGYRISRR
                     ALAPLSHLPYLFGTYL"
     gene            complement(1741..3168)
                     /locus_tag="WGQ85_002013"
     CDS             complement(1741..3168)
                     /locus_tag="WGQ85_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0751354.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3353..>4840
                     /locus_tag="WGQ85_002014"
     CDS             3353..>4840
                     /locus_tag="WGQ85_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0751355.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPPGKSNL"
BASE COUNT         1042 a         1355 c         1315 g         1128 t
ORIGIN      
        1 cgacaagttg gacgtgagcg gttttatcca ttaccgcatc aaccattcca aggagtttgc
       61 agaccgtcag aaccacatta acggcattga gaacttttgg aatcaggcaa aacgcgtctt
      121 gcgaaaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag
      181 atagtacgga accgatttac ttggtgcttc agcaccttag agaatcgttc tctttgagct
      241 aaggcgaggc aacgctgtac tggtttaaat ttaatccact atacaacgga atcgattgca
      301 aatctttccc actgttcttg aaagaatgcg aatttcgatt taacttcggc acaccgtctc
      361 aacagcttaa aatcctgcgg gattggtgtg ggatttaggg ctaatctagt acagcccctt
      421 ttaaaaaaca ttacaatccc gggccgccaa gcgaattggc aatgtccctg accgccgtta
      481 catacatccg gctgtggttg tactgccata ccgtataaaa attgttcaag cccaaataat
      541 attcaaacac gccgggtgcg gtttccagtt tgaacaaaac cgccttttca tcatcggcaa
      601 gctcttcgcc ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc gtcagggcgg
      661 ttttttcgcc aatgattgcc tgaacatccg cacccggcgc caatgttgca gacaccagta
      721 ttttcccgcc cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat gccgcgacat
      781 cgccgatgtt gccccatatg tcccgatgtc cgtccccgtc ataatccacc gcccatttcc
      841 ggtagctcga aggcataaat tgcggcatcc ccattgcgcc cgcatagctg cctttaaagg
      901 cgaaaacatc gccgccttct tcttttgcca gctttaaaag ctcgaccaat tctttttgga
      961 aaaacccggc gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc gccacacgga
     1021 aactgcccgt atttttgccg taattcgttt caatcccgat aaccgccacg ataagttcgg
     1081 caggcacgcc gtatttttgc gccacatcat cgataagcgc gcggttttcc gcataaaacc
     1141 ggcgcgcgcc gcgaaatttc gccttgcccg aatttcccgt gcggaacaca taccacggac
     1201 gcgatgtgga ggggcggtgc ataatcttga cgatgtccgc cttgtaagcc gctttgtcaa
     1261 aaaaatcctg ccattccgcc cgggaaaaat cccctttccc gacttcatcg tccacaaaac
     1321 ggcggacatt tgcattggcg gcaaacccgc tgtcggatac cggtacggct gccgcgtcaa
     1381 atacggctgc cgcgtcaaac gcggggcggc tttctttttt catttcatcc gcgcgggggg
     1441 cttgggcttc atttgcccgg ggcgggcgtg cctccatcgc cgtacaggca gacaaagccg
     1501 ccaaacaaat tgccagcggc agtatttttc tctttttcat aaatatgttc cgaacaaata
     1561 gggtaagtgg gaaagcggcg caagggcgcg gcgcgaaatg cggtatccgc cgcaatcggc
     1621 ggctttgcag aatgccgcgc gttgcctttt gcgccgcccg aaatctgcct gccgccgccg
     1681 aaaatgccgt ctaaaggcac ttcccctttc agacggcatt gcctgcgccg tatttgcccg
     1741 ctatccgcaa tatcggcagt ccaatatgtc tttgcggata tcgttcagca ggcaggctgc
     1801 cgcgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgataaa
     1861 gactttttgc agggcgcagg cgtcggtcag cgtgtgttcc tgcaggaagc tgcggatgtc
     1921 ttcgggtaag gtgtaatcac gggcaacggc ggcaaagcgg gcatcggttt gcaggcggtc
     1981 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
     2041 gacaaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
     2101 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
     2161 caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
     2221 gatgttcgcc tccatcagct gatgggcgac gggggcaaca ggcgaaacgg aatggcagac
     2281 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
     2341 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaaggatgt attccataat
     2401 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
     2461 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
     2521 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
     2581 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
     2641 ttctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt attttgcggc
     2701 gttgacgtaa atggtttgtc cgtcggtata ttcgtagtaa gactgcattt ctcgggcaat
     2761 cgccgcgccg gaggtttcgg gttcggtaac gcccaaacca ccgccctcgc ctttgaaaac
     2821 catgtccaaa ccctgtgcga tttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
     2881 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
     2941 caagacttcc tgaatttcaa actggctgcc cttgcgcccg ccgtgttttt tgtcgaggaa
     3001 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
     3061 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
     3121 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
     3181 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
     3241 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagacg gcatcgcgcc
     3301 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
     3361 ccccatcccc aaaccccgtg aaaaatcccg ttggtccaat ctttcgcaag gctcgctgcc
     3421 cttggctttg gcgcgttatc tgccgcataa gcggctcaag gtcgtgctga cccaagatgc
     3481 ggaacaggcg ctacgccttc agacggcatg gcggtttttc cgtccgcacg acacggcggt
     3541 gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tcgccgcatc aggatttggt
     3601 gtcggaacgg ctgtcggcgt tgtggcagat taagagcggc gcggcggacg tgttgttcgt
     3661 gccggttgcc acggcgatgc agaagctgcc gcccgtgccg tttctggcag ggcgcacgtt
     3721 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaaccgatt tggtagatgc
     3781 gggctacaac catgtttccc atgttgttgc ggcgggcgag tttgccgtgc gcggcggcat
     3841 tgtcgatttg ttccccatgg gcagcgaaat gccctaccgc atcgatttgt ttgacgatga
     3901 aatcgacagc atcaaaacct ttgatacgga cacgcagcgc accatctccc ccgtttccga
     3961 aatccgcctg ctgccggcgc acgaattccc caccgacagc gaggcgcaaa aaatcttccg
     4021 cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt acaaagccgt
     4081 cagcaacggc cacttcggtg cgggcgtgga atattacctg ccgctgtttt ttgaaaacga
     4141 gctggaaacg ctgtttgact atatcggcga ggatgcgctg tttgtctctt tgggcgatgt
     4201 tcacgccgag gcaaaccgct tttggagcga cgtcaaatcg cgttacgcca tggcgcaggg
     4261 cgatgaaact tatccgcctt tgcttccaca gcatttgtat ctctctgccg atgtattcgc
     4321 aggccgtctg aaaaactacg gacaagtgct gcccgatgtt tccggcaagg aacacatcct
     4381 gcccgacctt gccgtcaacc gccaatccga cgagccgttg caggcattga aggattttca
     4441 gacgaccttt gacgggcgga ttttgctgtg cgccgaaagc ttgggacggc gcgaaaccat
     4501 gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact ggcagggctt
     4561 cttgtcggcg catgagccgc tgatgattac agtggcgccg ttggcttacg ggtttaaact
     4621 gggcggactg caatcgccga accaacagca aactacttcc gcctccgaag gagaaggcga
     4681 tgcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc ctagccctct
     4741 cccacaggag agggaacaaa gtgcagccgc cgtttcagac agtctgaaag cagccgccgt
     4801 ttcaaccgaa agcagcctgc cccccggtaa aagtaatctg
//
LOCUS       DAWFIT010000122         4563 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFIT010000122 DAWFIT010000000
VERSION     DAWFIT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.122"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            22..256
                     /locus_tag="WGQ85_002015"
                     /pseudo
     CDS             22..256
                     /locus_tag="WGQ85_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            424..2199
                     /gene="ggt"
                     /locus_tag="WGQ85_002016"
     CDS             424..2199
                     /gene="ggt"
                     /locus_tag="WGQ85_002016"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0751356.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2418..2613
                     /locus_tag="WGQ85_002017"
                     /pseudo
     CDS             2418..2613
                     /locus_tag="WGQ85_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2680..3930
                     /locus_tag="WGQ85_002018"
     CDS             2680..3930
                     /locus_tag="WGQ85_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0751357.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4058..4563
                     /locus_tag="WGQ85_002019"
                     /pseudo
     CDS             4058..4563
                     /locus_tag="WGQ85_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1256 a         1130 c         1058 g         1119 t
ORIGIN      
        1 aggcacgccc cagtttcccc aatgtggatt ttcagcgcgt gcagtagagg tgctgaccca
       61 attggcagac cctttgcttt tgttaatatc ctagaaaacc cagaaattcg cagtactcta
      121 ccactcatcg ccaatcggcc aacctttcca cagctttggg ttaatggtga gttaatcggt
      181 ggtagtgata ttatcacaaa tgtaccaatc aggtgagcta aaaccactca ttgaagagca
      241 tagcccaaag gtctgattac ttaatatctt aaaataaata tcaaaaaaga ctgctgatga
      301 cattagcggt ctttttttga tggtcaagat tgatagtttg gattgaatta tttagtcata
      361 ttaaatacaa taatcagcat gccttgtatg aatcatcaat caaactcagg cgaaggagtg
      421 cttgtggcta aaacatattt attgactgca ttgataatgt ctatgacaat ctctggatgt
      481 caagtcatcc atgccaatca aggtaaggtt aatactcatt ctgctgtcat cacaggtgca
      541 gacgctcaca cgcctgaaca tgcaacggga ctgaccgaac aaaagcaggt gattgcaagt
      601 gattttatgg tagcgtcagc caatccatta gcaacacaag ctggctatga tatcttaaag
      661 caaggcggta gcgctgcaga tgcgatggtg gcggtgcaga cgacactaag cttggtagag
      721 ccacagtcgt caggcttggg cggtggtgca tttgtgttgt attgggataa taccgccaaa
      781 acattgacca catttgatgg gcgtgagacg gcaccgatgc gtgcgacgcc ggaattattt
      841 ttggataaag atggtcaacc attgaaattt atggaagcgg tggtcggtgg tcgctcggtg
      901 ggtacgcctg ctatccctaa actgatggag acaatacatc agcgatacgg tgtattgcct
      961 tggggaaaat tatttgatac accgatccgc ttggcaaaac aaggctttga ggtgtcgcca
     1021 aggcttgcca tctcggttga gcaaaatcag cagcatttgg cacgctatcc aaaaacagcc
     1081 gcttattttt tgccgaatgg tgtgccgcta caagcaggca gcttgctgaa aaatttagaa
     1141 tttgctgaca gtgttcaggc gttagcagct caaggtgcaa aagctctgca tactggtaaa
     1201 tatgcccaaa atatcgtttc agttgtccaa aatgctaagg ataaccccgg tcaattatcc
     1261 ttacaagatt tatctgatta tcaagtggtg gaacgcccgc ctgtttgtgt gacttatcgt
     1321 atttatgaag tatgcggtat gggtgcacca agctcaggag ggattgctgt gggtcagatt
     1381 ttggggattt taaatgaatt ttcacccaat caggtggggt atgatgctga aggtttacgt
     1441 ctcttgggcg acgcttctag gcttgcgttt gcggatcgtg atgtatattt gggcgaccct
     1501 gattttgtac cagtacccat tcgccagttg atttctaaag actacctaaa acatcgcagc
     1561 cagctgcttg agcagtcgga taaggcattg cctagcgtat ctgcaggcga ttttattcat
     1621 gagtgggtgt cctcacaagc gattgagtta ccttctacca gtcatatatc aattgtggat
     1681 aaggcgggca atgtgctatc aatgacgact tccattgaaa atgcgtttgg ctcaactttg
     1741 atggcaaatg gctacctgct gaataatgag ctgacagact ttagttttga gccaataaag
     1801 cagggcaaac aggtcgccaa tcgtgtcgag cccggcaaga gaccacgctc ctcaatggca
     1861 ccaaccatcg tatttaaagc aggcaagcct tatatggcaa tcggctcccc aggtggtagc
     1921 cgtatcattg gctatgtcgc taagacgatt gtggcacata gtgactggaa tatggatatc
     1981 caagatgcca tcagtgcacc caatttatta aatcgctttg gtagctatga actagaaacg
     2041 ggtacaaccg ccctccagtg gcaacaagca ttaaatgatt tgggctataa gactgacgtg
     2101 cgtgagctaa attcaggtgt tcaagccatt attatcgagc caagcaggct ggttggtggt
     2161 gccgatccac gccgagaagg tcgagtgatg ggtgattaga ggtgaagctt gtattttgtg
     2221 aatttgaccg cattaagtta atattaaaat agggggaaat tgtgagtaat caaacaaaaa
     2281 tacaagccat caagcaagct tctgagcaga tcttcgcaat ttgtgagacg ccaaacacag
     2341 cattacaagc cattcatctg attttaagac atggtggggc aggagagttg tcttggcaag
     2401 tggtgtatca gcgggttatg gctgataaag atgtggttgg tgctggttat ttgattgatt
     2461 ttgcacaaac tgccgaaaat ttaccatttg atgcattgcc tttgggtagc cttgtactaa
     2521 cataaaggta atgagacgct aaaaaccagc atgttaaata aattacctga taatgccaaa
     2581 gaaaatttac gcattatgga ctatctgcct taggttttga agtatttttt tatacaaaaa
     2641 acactcatga taccctgatt acacaaagga gccgagaaca tgttttcaaa aagcgttacc
     2701 ctcgcacaat atgaccccga tttggcagca gccattgccc aagaagacca acgccagcaa
     2761 gaccacgtcg agctgattgc ctctgaaaac tacgtcagct gcgccgtaat ggaagcgcaa
     2821 ggttcccagc tgaccaacaa atacgccgaa ggctaccccg gcaaacgcta ctacggcggc
     2881 tgcgaatacg tcgatattgt cgaacaattg gcgattgacc gcgtcaaaaa actgttcggc
     2941 gcgcaatacg ccaacgtcca accgcactcc ggctcgcaag ccaaccaagc cgtgtacgct
     3001 tccgttttaa aaccaggcga caccattttg ggtatgtctc tggcgcacgg cggccacctg
     3061 acccacggcg cgagcgttaa tatctcaggc aaactctaca acgctgttac ttatggtttg
     3121 gatgaaaacg aagtgctcga ttatgccgaa gtcgaacgcc tcgcgctcga acacaaaccc
     3181 aaaatgattg tggcgggtgc gtctgcctac gcgttgcaaa tcgactgggc aaaattccgc
     3241 gaaatcgccg ataaagtcgg cgcatacctc tttgtcgata tggcgcacta tgccggtctg
     3301 gttgccggcg gcgaatatcc caatcccgtg ccgttctgcg acttcgttac caccaccacc
     3361 cacaaaaccc tgcgcggtcc tcgcggcggc gtaattttgt gccgcgacaa tacccacgaa
     3421 aaagcgttga actcttccat cttcccaagc ctgcaaggtg gcccgctgat gcacgttatc
     3481 gccgccaaag ccgtggcgtt taaagaagca ttgcaacccg agttcaaaca atacgcaaaa
     3541 caagtgaaaa tcaatgcagc cgccatggcg gaagagttgg ttaaacgcgg tttgcgcatc
     3601 gtttccggcc gcaccgaaag ccacgttttc ctcgttgacc tgcaaccgat gaaaattacc
     3661 ggcaaagccg ccgaagctgc tttgggcaaa gcgcacatca ctgtcaacaa aaacgccatc
     3721 ccgaatgatc cggaaaaacc cttcgttacc tccggcatcc gcatcggttc cgccgccatg
     3781 accacacgcg gttttaacga agccgacgcg cgcgtattgg cgaacttggt tgccgacgta
     3841 ttgtctaacc ccgaagacga agccaacctc gccaaagtcc gcgaacaagt aactgctttg
     3901 tgcaataaat accccgttta cggcgcgtaa gcctttttaa aaatattccg ctaagcaatc
     3961 caatgccgcc tgaaatctca taatgtttca ggcggaaacc tttgcaaaat tccccaaaat
     4021 cccctaaatt cccaccaaga catttagggg attttccatg agcaccttct tccggcaaac
     4081 cgcgcaagcc atgattgcca aacatatcaa ccgtttcccg ctattgaagt tggaccaagt
     4141 gattgattgg cagccgatcg aaccatacct gaaccgtcaa agaacccgtt acctccgaga
     4201 ccaccgcggt cgtcccgcct atcccctgct gtccatgttc aaagccgttc tgctcggaca
     4261 atggcacagc ctctccgatc ccgaactcga acacagcctc atcacccgca tcgacttcaa
     4321 cctgttttga cgttttgacg aactgagcat ccccgattac agcaccttat gccgctaccg
     4381 caaccggcga cgcaagacga caccctgtcc gaattgccgg aactgatcaa ccgccaactg
     4441 accgaaaaaa acctaaaaat agagaaagca tccgccgccg tcgttgacgc caccattatt
     4501 cagaccgaga cctttgcaaa attcctttcc ctcccgacag ccgaaaccca aacacaggtt
     4561 ttc
//
LOCUS       DAWFIT010000123         4214 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFIT010000123 DAWFIT010000000
VERSION     DAWFIT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.123"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGQ85_002020"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGQ85_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0751358.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WGQ85_002021"
     CDS             1384..2148
                     /locus_tag="WGQ85_002021"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0751359.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WGQ85_002022"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WGQ85_002022"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0751360.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         1002 a         1153 c         1156 g          903 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acctcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtt tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctggctcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcgaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattct gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagtgct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt gggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga gcaaatcaaa
     1501 aatatgccct tcgatattgc ggagttccgc gccgactttt tggaatgcgc gggcagtatc
     1561 ggcgaaatat tgcaccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc tcgttcccgt gttcggacga ttattatttt
     1681 gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 tccggcgaaa ccgccgtccg gtgcgccgtg gcaaatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcaaagcga aagagcttgc cgccaaaccg
     1981 attgttacga tgtcgatggg gcagacgggg gcggtcagcc ggcttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcgacact cgactgcctc gaaaacggag cagactgatt tcagacagca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgctacc tcggcggccc
     2221 actgctcgtc cttgccggtg caggcagcgg caaaaccggc gtgattactc aaaaaattaa
     2281 gcatttgatt gtcaatgtcg gctacctgcc gcataccgtt gccgcaatta cctttaccaa
     2341 caaagccgct gcggaaatgc aggagcgcgt tgccaaaatg ctgcccaaac cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccactcttt gggcatgaag attctgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttc gacgacttga tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgcaacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg aacatggaaa acctgcgcaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgagaac aaccccaagc tgttcaccaa
     3061 aaaactttgg tcgaaattgg gcgaaggcga gcccgtcaaa gtcgttgcct gccaaaacga
     3121 gcagcacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt ataccgcgga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gaaccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc gacagcatga aaaaaggcgg tccggaggat taaaccaaga tagggaaacg
     4201 aagccggaga caat
//
LOCUS       DAWFIT010000124         4111 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFIT010000124 DAWFIT010000000
VERSION     DAWFIT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.124"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..243
                     /locus_tag="WGQ85_002023"
     CDS             <1..243
                     /locus_tag="WGQ85_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751361.1"
                     /translation="RLKTEGGSLSSLPSDGISNAAFAPYAGGSPPSFQCLRRMPVASG
                     KPCAGWLCRLKQYEGMRFRRHPPVAEIPSASCPIIA"
     gene            complement(233..1426)
                     /locus_tag="WGQ85_002024"
     CDS             complement(233..1426)
                     /locus_tag="WGQ85_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0751362.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1423..2391)
                     /locus_tag="WGQ85_002025"
     CDS             complement(1423..2391)
                     /locus_tag="WGQ85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0751363.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAVPQTGETEWKQD
                     TEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWP
                     KTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVERILEAAH
                     THRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGEDRYTDSV
                     NVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2402..3838)
                     /locus_tag="WGQ85_002026"
     CDS             complement(2402..3838)
                     /locus_tag="WGQ85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0751364.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(3968..>4111)
                     /locus_tag="WGQ85_002027"
     CDS             complement(3968..>4111)
                     /locus_tag="WGQ85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0751365.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT          850 a         1042 c         1332 g          887 t
ORIGIN      
        1 cgtctgaaaa cggaaggcgg cagtttatca tctttgcctt cagacggcat atcaaacgcc
       61 gcctttgcgc cgtatgccgg aggttcgccc ccgtccttcc aatgcttgag gcgaatgccg
      121 gttgcgtccg gcaaaccgtg cgcggggtgg ctatgccgtc tgaagcaata tgaaggtatg
      181 cgtttcagac ggcatccccc tgttgcggaa ataccgtccg cctcctgtcc gattattgcc
      241 tgattgcagc ggcgcggacg agttcttcga ggctgtcggc gagcatttcc gccgcgcgcc
      301 ggtagccttt ggcggagaag tgtacgccgt ctttggcggc ccatccttgg ttgagccagt
      361 ttttcatgct gcatatgccg cccatggcgt tttgccaaga ccagaacatg gtttgtccct
      421 gccgggcgac gcgccgctgc atatgttgga cttcggtcag gcggacgggg cgtgtgccgc
      481 atacgccgag cgtgtttttc agggattcgg gtgcgccgat gatgaggatg ccggcggcag
      541 gcaggctgtc gtggatttgg cggacggtat ccagccattt ttgttcagtg tcggcaatgt
      601 cgatgttgtc gccgaaggct tcgttggtgc cgtaggaaag gataaccaaa tcggcgccgg
      661 tttgggcaag gtcgttcata cggtcggcac gccatttcga ccaatgggtc aattgtgcgc
      721 cgttgatgcc catggcggaa acggtaatgc cgccggcggg attttcgatg ttgatgaagc
      781 cgatgtccca cggcatttcg gtctgtatgg tcaagggcag tgccgcgcgg gtatccagta
      841 cctgccagcc gccgccgttg gcggagacgg tgttgccgtt gacggtcagg gtttgttcgg
      901 cgagcagggg tttggcaaac agggaaatgc gctgtttgcc ggtcttgccg tccgatgcgg
      961 tcagggtcat gctgccgccg ctgccggtgt gggcgaggat gccgccgagc gggaagtctc
     1021 cgctattgtt cctgctggtg aggctttgcc agttaccgtt gtgccggacg gccgccatgc
     1081 gctgcccttt gacgttggcg gggtaaaccc agcctatgcc gccgtcgccc caagtttttt
     1141 gcaggcgttt gcgcaggctg tcggtaaaga agtcgccggc ggtatgcgag tcgccgattt
     1201 gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga ttggagtttt ttcatccaag
     1261 gcgaggcggc ggcgttgccg tagtcggtca gcagcccgtt tgtatcggtg tagggggcgg
     1321 acggggaaac ggtaacggtg tcgagactga cggaggcaac aggtagggcg tttgcctggg
     1381 ttgcggcgaa cagggcggaa aatgcgatga ggtgtttggg gttcatggct gtgtacttga
     1441 tggttgcgta cttggttcaa aaacgatttt ttccattatt tttgccgcca gcagtttttg
     1501 tccttcggcg gtaaagtgta tgccgtcctt gctgcggtag cggacgggtt tgccgttgac
     1561 gttgacggag tccgtgtagc ggtcttcccc gccgctcagg gtgtgcgcgg tgggaatcag
     1621 gatgattttg cctttcaaat gttccgaaag cagtttgtcg aggtagcgca tctgcccgtc
     1681 aagcttggct tttttcatgt aggggatgcc gagccagacg acttggacgc ggtgcgtgtg
     1741 tgcggcttca aggatgcgct cgacgcgttt caggtattct tgcgcccatt cgtcggaagc
     1801 aaatttgagg tagcgtttgc cgacgggaaa atcccacggg tcgttcgggc cgaggaagac
     1861 ggcgagtacg ctgatttcgg gatgtttttg cagggtttct tcaatcgttt tcggccagtc
     1921 gaagaatgag gggtaggaca gccccgtgct ttgtttgctg aggttgacgg attcgatgcc
     1981 gtattgctgt ttcaggcttt tttgcacgaa gggtgcaacg ccctgcatca gcgagtcgcc
     2041 ggcgaaaaag actttgtcgc cgctgcggac ggcggcggct tcggtgtctt gtttccattc
     2101 tgtttcaccg gtttgaggga cggcttcaga cggcatatct gccgagccgc cgtcttgagc
     2161 cgtcggcggc gtttcgccgg acaggaatgt tttgatgccg tctgaaaagg cgtaggcgtt
     2221 ttcttgcaac gccgcaccgc tccgccacca tccgtaggcg gcaagcggtt cgagcggact
     2281 gttgcggtgg taggtctgct gccagtaggc gttgatgggg ttttggctga accacacggc
     2341 aatcagggca gacatcaata tggaagtgaa aagggaaaga aagtgtttca tgtccaaacc
     2401 cttaaaaatt ggcataaata aagccgggaa tccccgaggg ggcgaatacg ataatcagca
     2461 gcaggacggc ggaaacgggg ataaaccaca gccacatcgg gattttttcc aaacctttga
     2521 ccgcgccgtc gaaagcgcgt tgcaggtaag ggtagagcag catcacggat gcaaacgagg
     2581 caagcagcag catatccgcc cgttgcggcg cattccagcc gttggcattg gcaaagaggg
     2641 cattgaaaac tgcgcctgca tcgtcggggt ttgcggtatt gaagacgacg aaggtcaggc
     2701 agacgaaatg gaaggtaaca aaccacgaga gcggcgcaag gtatttcaga cggcatagtg
     2761 cgttgcgtcc gaaatagcgg tcgcccgtgt tgagcagcac cagcgccgtg ccgtgcagcg
     2821 cgccccaaat gaggaagttc cagccgtagc cgtgccagat gcctgagagt accattgccg
     2881 ccatcaggtt gagctgtgtc cgtaaaaagc cttttttgct gccgcccaag gggatgtaga
     2941 tgtagtcgcg tatccaggtg gaaaggctga tgtgccattt gtcccaaaat gcacggatat
     3001 tcgcggcacg aagcggtgct gagaagtttt tgggcagtcg gaagcccagc agcattgcca
     3061 tgccgataac caaatcggaa tatccggaaa agtccaggaa gagttggaag gtatagccgt
     3121 acacgccgcc caatacgccc cagccgtcga attgggcggg gttttcaaat acgggcgata
     3181 cccagttttc cgccagcatc cccgccagcc accatttttt ggcaataccc agcagaatca
     3241 gggaaacggc gagtgcggga cggacgggcg aacgcggttg gcgggtacgg atttgcgcca
     3301 atgcgcccgc ctgttcgccg tccgcgcttt tgaatgcggc ggcgcggata atcgggccgg
     3361 aggtaacggt ggggaaaaaa ctcaggtgca gcagcagttc gtgccactcg aaacgcgcgg
     3421 cgtgcggcgc acggaagcag taaaccaaat aggcgattga ctggaaggtg taatacgaaa
     3481 gccccagcgg catcaggatg tcgattgcgc cgccttgtcc ggcatattgt gcaatcagcg
     3541 ggcggaaaaa gtcgaaatat ttgaaaaagc ccaagacggt cagcgagacg gcaatgccgc
     3601 accccagcca aaagcggcgc gtattttcgc gatcggaacg gagcagttcg cccaaaaggt
     3661 acacgcagga ggaataaagg acgacgattg ccgcaaatac ggggccgata tggtagagcc
     3721 agcccatacc ggcagccaaa agcagcaggt tttggacgga cgggtatttc gccaagcccc
     3781 agtaaatcgg caggaaggcg agaaagaaca gtgcgaactc gacagacagc agcggcataa
     3841 accatccttg gaataagaaa ataggcgtta aaacacggca aagcccgtta ttttaagcag
     3901 gggcggatat ttcttcaatc aaaatgccgt ctgaagggcg ggaggggctt cagacggcat
     3961 cgcgcggtca ggtttggata tggcggttca agttcaggca cagccacagc ccggccgccg
     4021 ccgcaagcat accggcgatg ccgatacggg aaatgccgac atattgcgtt acccaatgtc
     4081 ccaacagtgc gccgctgccg ataccgacgt t
//
LOCUS       DAWFIT010000125         4084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFIT010000125 DAWFIT010000000
VERSION     DAWFIT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.125"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(240..665)
                     /locus_tag="WGQ85_002028"
                     /pseudo
     CDS             complement(240..665)
                     /locus_tag="WGQ85_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            813..1265
                     /gene="dut"
                     /locus_tag="WGQ85_002029"
     CDS             813..1265
                     /gene="dut"
                     /locus_tag="WGQ85_002029"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0751366.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1341..2528
                     /gene="dapC"
                     /locus_tag="WGQ85_002030"
     CDS             1341..2528
                     /gene="dapC"
                     /locus_tag="WGQ85_002030"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0751367.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2686..3465
                     /gene="yaaA"
                     /locus_tag="WGQ85_002031"
     CDS             2686..3465
                     /gene="yaaA"
                     /locus_tag="WGQ85_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0751368.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRKLAPQ
                     QIAELMHVSDKIALLNAERNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLMQPYRLEMGTPFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3548..3638
                     /locus_tag="WGQ85_002032"
     tRNA            3548..3638
                     /locus_tag="WGQ85_002032"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3582..3584,aa:Ser,seq:tga)
     gene            3716..3805
                     /locus_tag="WGQ85_002033"
     tRNA            3716..3805
                     /locus_tag="WGQ85_002033"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3750..3752,aa:Ser,seq:cga)
BASE COUNT         1050 a          982 c          991 g         1061 t
ORIGIN      
        1 gtgggaatct agttttttga gtttcagtca ttttcaataa attgccttag cattgaatgt
       61 ctagattccc gcctgcgcgg gaatgacgat tcatatagtg gattaacaaa aatcaggaca
      121 aggcggcgaa gctgcagaca gtacagatag tacggaaccg attcacttgg tgcttcagca
      181 ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ctgtactggt ttttgttaat
      241 ccactataaa aaggcatatt gaatgcgggc aaaccggctg ctttccgttt ttggatttcg
      301 gagaatgcca tcgcccagct ttcatcacac ataaaaaaca gtgcgggcac ggcttttttc
      361 agcggtattt ctttcaggtg cagggcaagt gccgccccca tcaggatatg ccgcgaatta
      421 atcataaagg ttacggtggc gataagcagt atcggcagag attccgccca caggttgacc
      481 gtggcaaact cggagccgcc ggcgaagttc atactggtca tcaacaacat ttccagccag
      541 ctcatgcctt tttgtccgcc ctgcataccg agtattaatg cccaaggcag aagtccgata
      601 agtatgggcg aactttcttt gatgccgcgt ataaattcgt tacggggcga gcttatgtgt
      661 gtcatattat aatattgata tgtaagaagg tcgggcatta tacacagacg gagtatttca
      721 aaacaaaacc tatttgctgt gtttcagtgt aaacacggct tgtgtataat ccaccatctt
      781 tagacagacc gaccgtatgt gggagcaagg caatgaatat tgaagtagaa atgaaagtgt
      841 tggacgagag gatggcggat tttatcccta cctatgcgac agcgggttct gccggtttgg
      901 acttgcgcgc ctgtttggat gaggaagtcg tcttgcagcc gggggaaacg tttcttgtgc
      961 cgacaggttt ggcaatttat ttggccaatc cttcatatgc cgccgttttg ctgccccgtt
     1021 ccggcttggg gcataaacac ggcattgttt tgggcaattt ggtcggcttg attgactccg
     1081 attatcaagg ggaattgaag gtttcgttat ggaacagaag cagcgagccg tttactgtca
     1141 aaccgtttga gcgtatcgcg cagatggtta tcgtgccaat cgtgcaggcg cgtttcaaac
     1201 gtgtcgagga atttgtcgga agcagccgtg gagaaggcgg tttcggcagt acgggtttgc
     1261 attgaatata aaatgccgtc tgaagggcac gtcaggttca gacggcatat cttccgatat
     1321 accgataacc ggagaaaatc atgaatacct tactcaagca gctcaaaccc tatccctttg
     1381 cccgactgca cgaggcgatg cagggcattt ccgcgcctga aggcatggaa gccgtccccc
     1441 tgcacatcgg cgaaccgaaa catccgacac cgaaagtcat tacggatgcg ctgaccgcct
     1501 cattgcacga gttggaaaaa tatccgctga ctgcgggtct gcctgaactg cgtcaggcgt
     1561 gtgcaaactg gttaaaacgc cgttacgatg gcttgacagt ggatgcggat aatgaaattc
     1621 tgccggtttt aggcagtagg gaggcgttgt tttcttttgt tcaaaccgtg ttgaaccctg
     1681 tttcagacgg catcaaaccc gcaattgtca gcccgaatcc cttttatcag atttacgaag
     1741 gtgcgacact tttgggcggc ggtgaaatcc attttgccaa ttgccccgcg ccgtctttca
     1801 accccgattg gcgtagtatt tccgaagagg tttggaaacg caccaaactg gtgttcgtct
     1861 gctcgcccaa caaccccagc ggcagcgtgc tggatttgga cggatggaaa gaagtttttg
     1921 atttgcagga caaatatggt ttcattattg cctcggacga atgctattcc gaaatctatt
     1981 tcgacggcaa caaaccttta ggctgcctgc aggccgctgc acagttgggg cgaagcaggc
     2041 aaaaactgct tatgttcacc agtttgtcca agcgttccaa cgttccgggc ctgcgttccg
     2101 gttttgtcgc cggcgatgcc gaactgctta aaaactttct gctttacaga acctatcacg
     2161 gcagtgcaat gagcattccc gtgcagcgcg caagcattgc cgcttgggat gacgaacagc
     2221 acgttatcga caaccgccgc ctgtatcagg aaaaatttga gcgcgttatt cccattttgc
     2281 aacaggtatt tgacgttaaa ttaccggatg cctcgtttta catctggttg aaagtccccg
     2341 atggcgacga tttggcattt gcacgcaatt tatggcaaaa agccgctatc caagtattgc
     2401 ccggacgttt tttggcgcgg gataccgaac agggcaatcc cggggaaggt tatgtccgta
     2461 tcgctttggt tgccgatgtc gcaacttgtg tcaaagctgc ggaagacata gtttcactgt
     2521 atcggtaagg aatcggcaat gcagtctgaa cttttgttca gacggcattt ttcaatattt
     2581 tacggttgaa tttgctgtaa cggtatttac ttcactttta atcggcttgc ccgcaaacac
     2641 gtttaaactt aaaatcccca tgtttgacac aataccgagc ggattatgtt ttttgtcctt
     2701 tcccctgcga aaaaccttaa cgaaaaagac cctgcccctg tcagtgagtt tacccaaccc
     2761 gacctgctgg cagagtccga aattctaatg cagcagttgc gcaagcttgc gccgcaacag
     2821 atcgccgaac tgatgcacgt ttccgacaaa atcgcactct taaacgccga acgaaatgca
     2881 gaatggcaca cgccgtttac gccggaaaac gccaaacagg cggtctttat gttcaacggc
     2941 gatgtttacg aaggtatgga tgcaaacaca ttggatattg gacagatacg ctatctgcaa
     3001 aaccatgtcc gcctgctgtc cggtctgtac ggtcttctgc gtccactgga tctgatgcag
     3061 ccctaccgcc tggaaatggg aacgcccttc gccaatttgc gcggcaagaa tttgtatgag
     3121 ttttggggcg acatcattac caacctttta aatgacacgc ttgcccaagc aggcagcaat
     3181 acgcttgtca atcttgcctc acaggaatat tttaagtccg tcaacacgaa aacacttcgg
     3241 gcgcggctga ttaccccgat atttaaagac gaaaaaaacg gcaaatataa aattatcagt
     3301 ttctatgcca agcgtgcacg cggattaatg gtgcgctatg cggcagaaca tcatattacc
     3361 gaccctgaaa tgctgaaaaa ttttaattac gaaggctacg cattcaatga cgcggcttca
     3421 aatgaaagcg aatgggtttt tatgcgttcg gaacaaataa agtgaaaaca ataaattaag
     3481 tattttccaa aaaaagtgct tggcaaaatg tataaacttc attattattc ctaatcttca
     3541 agaagacgga agcgtggcag agcggtttaa tgcaacggtc ttgaaaaccg tcgagggttg
     3601 atagccctcc gtgagttcga atctcaccgc ttccgccaat ccttgaggtc aaaaaccaaa
     3661 taaaaatgaa gtagcataaa gcatcggcat ttttatttgg ttttacttca ttatcggaaa
     3721 cgtggcagag aggctgaatg cagcggactc gaaatccgct gagggtgcaa atcctccgtg
     3781 ggttcgaatc ccaccgtttc cgccacaaaa caaaaccgcc ctgattcggg gcggttcttt
     3841 tttgttcaag ttgagacctt tgcaaaattc ccttccctcc cgacagccga aacccaaaca
     3901 caggttttcg gctgttttcg ccccaaatac cgcctaattt tacccaaata tccccttaat
     3961 cctgcccgga tacccgataa tcaggcatcc gggctgcctt ttaggcggca gcgggcgcac
     4021 ttaacctgtt ggccgctttc aacaggttca aacacatcgc cttcaggtgg ctttgcgcac
     4081 tcac
//
LOCUS       DAWFIT010000126         4026 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFIT010000126 DAWFIT010000000
VERSION     DAWFIT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.126"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            156..1673
                     /locus_tag="WGQ85_002034"
     CDS             156..1673
                     /locus_tag="WGQ85_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0751369.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1734..3614)
                     /locus_tag="WGQ85_002035"
     CDS             complement(1734..3614)
                     /locus_tag="WGQ85_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0751370.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQKGWQ
                     KGLHAVLFADYEFGLPLTGVDSERGGNLAMHWFADCADIDAASWLAQHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENRRCALLDRHLCRLKTAAQALN
                     LPLPDGCENQIKQYIAHLPDGAFRIKALLASDGISLSRAVLNHLADKQRVIISPTILP
                     AQNYLRRFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          833 a          910 c         1317 g          966 t
ORIGIN      
        1 ctataaatgt gaaatccgcc ctttgaaaat cgggcggcgg ttttttgcct gcccgctttc
       61 agacggcaaa gccggttttc acgggtttct gcctgttttt cggatggttt gacgtgcttc
      121 ggcggcgtgt ttgccagaaa ggtaaatgac agggtatgtt gtatttcaga tacggttttt
      181 tggttgtttg gtgtgcggca ggtgtttctg ccgcctatgg ggcggatgcg cccgcgattt
      241 tggatgacaa ggcattgttg caggtgcagc ggtcggtgtc ggataagtgg gcggaatcgg
      301 attggaaagt tgaaaatgat gccccgcgcg tggttgacgg ggattttttg ttggcgcatc
      361 cgaaaatgtt ggaacatagt ttgcgcgacg cgctcaacgg caatcaggcg gatttaatcg
      421 cttcgttggc ggatttgtat gccaagctgc cggattatga cgcggttttg tacggcaggg
      481 cgcgggcttt gctggcgaaa ttggcgggaa ggccggcgga ggcggtggcg cggtatcggg
      541 aactgcacgg ggaaaatgcg gcagacgagc ggattttgct ggatttggcg gcggcggagt
      601 ttgacgattt ccggctgaag tcggcagaaa ggcattttgc ggaggcggca aaattggatt
      661 tgccggcgcc ggttttggaa aatgtggggc gttttcggaa aaaaacggag gggctgacgg
      721 gctggcgttt ttcgggcggc atcagtccgg cggtcaatag aaatgccaat aatgccgcgc
      781 cgcagtattg ccggcaaaac ggaggccggc agatatgcag tgtcagccgg gcggagcggg
      841 cggcaggctt gaattatgaa atcgaggcgg aaaaactgac ggcgttggca gataatcatt
      901 atttgttgtt ccgttccaat atcggcggca cgagctatta tttcagtaaa aaatcagctt
      961 atgacgacgg gttcggcagg gcgtatttgg gttggcagta taaaaatgca cggcagacgg
     1021 cggggatttt gccgttttat caggtgcagt tgtcgggcag cgacggcttt gatgcgaaaa
     1081 caaaacgggt aaacaaccgc cgcctgccgc cgtatatgct ggcgcacgga gtcggcgtgc
     1141 agttgtccca tacttaccgc ccaaacccgg gatggcaatt ttcggtcgcg ctggaacatt
     1201 accgccaacg ctaccgcgaa caggataggg cggaatacaa taacggcagg caggacgggt
     1261 tttatgtttc gtcggcaaaa cgtttgggcg aatcggcaac tgtgttcggc ggctggcagt
     1321 ttgtgcggtt tgtgccgaaa cgcgaaacgg tgggcggcgc ggtcaataat gccgcctacc
     1381 ggcgcaacgg tgtttatgcc ggctgggcgc aggagtggcg gcagttgggc ggtttgaaca
     1441 gtcgggtttc cgcgtcttat gcccgccgca actataaggg cgttgcggct ttctcgacag
     1501 aggcgcaacg caaccgcgaa tggaatgtct cgctggcttt gagccacgac aagttgtcgt
     1561 ataaaggtat cgtgcccgcg ttgaattatc gtttcggcag gacggaaagt aatgtgccgt
     1621 atgcgaaacg ccgcaacagc gaggtgtttg tgtcggcgga ttggcggttt tgaatggtgg
     1681 aataatgccg tccgaacttt gtggaaacag gttcggacgg catttttgcg cgttcaggca
     1741 agggcggcgg caaatacgcc gcgcaaggcg ttggagaggc ggatttcttc ggcttcttgc
     1801 agtgtttttt gtgtgatgtg tgtttcgatt acttgatttg tttgcaaata tttttgcggt
     1861 tcgtccaata cggcttggcg cattatgccg tttaaaatgt ctaaatctaa agacggtgtg
     1921 agccattgcc cttggtattt gacgaacacg ttgcttctgc cgccttcgag caggatgccg
     1981 tctgaattga aaaacaggct gtcgaacgcg ccttgtgttt cggcggtttg ccacgcttgg
     2041 tcgaagaggg cgcggtgggt ggttttgaag cggcgcaggt agttttgtgc gggcaggatg
     2101 gtcggcgaaa tgatgacgcg ctgtttgtca gccaaatggt ttaaaacggc gcgggacagg
     2161 ctgatgccgt ctgaagcgag cagggctttg atgcggaacg cgccgtcggg caagtgggcg
     2221 atgtattgtt tgatttgatt ttcgcagccg tcgggcaggg gcaggttgag ggcttgggcg
     2281 gcggttttca gacggcatag gtggcggtcg agcagggcgc atcgcctgtt ttccacgcgc
     2341 agggtttcaa aaatgccgaa atcggggcgc aattcgttga ggaaacgggc tttccagccg
     2401 cattcgcgat attcggcggc ggggtcgctg tcgatgacga tgccggaacc gacaccgtac
     2461 acgccccgat acggcggatt ggagtcaaac ggatgcggcg ttgccccgcc ttgccctgcc
     2521 gttcgggctt gcgcgtttga atccgggccg ccgatgcctg aaacaatgcc gtctgaagcc
     2581 gaaacgggtt tgagcgacaa ggtgcggatg acgacgttga atatgccttc aaaccccaac
     2641 ccgcccgcgc aaggtttcag gtagccgatg ctgcccgtat aaagcccacg cgcttcggtt
     2701 tcgagcgatt cgataatctg catactcatt tttttgggcg caccggtgat gctgccgcag
     2761 gggaaggcgg cgcggaggat gtcggcgaac gaggtgtttg gcagggcttg ggcttggatg
     2821 gtgctggtca tctgccagac gctgccgaaa cgcgatactt taaacggctc gggcacgcat
     2881 actttgccgg tttgggcgat tttgccgagg tcgttgcgca gcaaatcgac aatcatcacg
     2941 ttttcggcgc ggtttttcgg gtcggtttgc aactcggcgg cgcggcgttc gtcttgtccg
     3001 tcgcccaaaa tcggcgcagt gcctttcatc ggttcggtgc tgatggtgcc gtctgaagcg
     3061 atgttgagga agagttcggg cgagaaacac agcgtccacg cggattgccc ctccacatcg
     3121 ggcaggtggg acaagacggc ataggggacg ggctggcgca ggcggcggta gaggctgacg
     3181 ggattgccgt aggcttgcag gtgcaggcgg gtggtgtagt tgatttgata ggtgtcgccg
     3241 cgccggatgg cttcgtggat ttggcggatg tggtcgaggt aatcggtttc ggatacggag
     3301 gattgcggcg tggaaatgcc ggcggggatg ccgtctgaat gttgggcaag ccagcttgcg
     3361 gcatcgatgt cggcgcagtc ggcaaaccag tgcatggcaa gattgccgcc gcgttcggaa
     3421 tcaacccccg tcagcggcaa accgaattcg tagtctgcaa acaacacggc atgcagccct
     3481 ttttgccagc ccttttgcag cgcgccgtcc aaagcatcga gttcttcggg acggaaaaaa
     3541 cggctttcta catgattttg atagcgtttt gcgcggccgc ttacggcatc gtcaaacagg
     3601 gcgaaatagg gcatggcaat tcggggcaaa tgttttgatt atacgctctt tttacacata
     3661 ttttcagacg attcggcaaa tatcggcaaa atgtaatttt atgtagagaa agtggggggc
     3721 gaaggtgtaa aattgtgaaa acaaattccg atttccaacc tgaaatacaa taaggagacc
     3781 tttatgacag accaccagct gcaaccgttt gaaaacgtag aattaggcga aaagcaagac
     3841 cagctccaag tatagtggat taacaaaaac cagtacagcg ttgcctcgct ttagctcaaa
     3901 gagaacgatt ctctcaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
     3961 tcttcggctt cgttgtcttg tcctgatttt tgttaatccg ctataaagac cgtcgggcat
     4021 ctgcag
//
LOCUS       DAWFIT010000127         4020 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFIT010000127 DAWFIT010000000
VERSION     DAWFIT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.127"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="WGQ85_002036"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="WGQ85_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0751371.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGEPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1587..3605
                     /locus_tag="WGQ85_002037"
     CDS             1587..3605
                     /locus_tag="WGQ85_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0751372.1"
                     /translation="MNKSLSSSVEEYCELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3649..>4020)
                     /gene="rfbC"
                     /locus_tag="WGQ85_002038"
     CDS             complement(3649..>4020)
                     /gene="rfbC"
                     /locus_tag="WGQ85_002038"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0751373.1"
                     /translation="QTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLSAQNQ
                     CQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIGWPLQTAPLLS
                     PKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          847 a          913 c         1193 g         1067 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccggaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgacaagg aaaagcgcgc tatgtacgac cagtatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggcggtg
      541 cgcagggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgcg
      601 cccagcctga ttatcagggt gaggacgttc aagtcggtat cgaaatcacg cttgaagaag
      661 ccgcaaaagg tgtgaagaaa cgcatcaata ttccgactta tgaagcgtgt gatgtctgta
      721 acggcagtgg cgcgaaaccg gggacatccc cggaaacctg cccgacttgc aaaggttcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcagca gacttgtccg acctgccacg
      841 gtgcgggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg cggaataagg
      901 cggtcaagac ggtggaagtc aatattcccg ccggtatcga tgacgggcag cgtatccgtt
      961 tgagcggcga aggcgagccg ggtatgcacg gtgcgcctgc cggcgacctg tatgtaaccg
     1021 tccgcattcg ggcgcataag attttccaac gcgacggtct ggacttgcat tgcgaactgc
     1081 cgatcagttt tgccacggct gctttgggcg gggagttgga agtgccgacc ttggacggaa
     1141 aggtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgt gtgaaaggta
     1201 agggtgtcaa atcgttgcgc agcagcgcga ccggcgattt gtactgccat attgttgtcg
     1261 aaacgcctgt caatttgacc gaccgtcaaa aagagctttt ggaagagttt gagcggattt
     1321 ctaccggctt ggaaaaccaa acaccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagccgtat cggggaatct ccttgatacg
     1441 gctgttttta tttgtttaaa aatagttttt attttcaatg gggtatgagg cagggtggga
     1501 taactgtttt taactgttct ttttaaaact tgacatcatg gcgtgatgcc aacaatatgt
     1561 gaacgtctgt tgtcaaagga agaataatga ataaatcttt atccagttcg gtagaagaat
     1621 actgcgagct gacgctccga ggcatgatac tcggtgcatt gatcactgta atttttactg
     1681 cgtccaatgt ttacctcggt ttgaaagtcg ggctgacctt tgcctcgtcg attccggcgg
     1741 cggtgatttc gatggcggtt ttaaagtttt tcaaaggcag caatattttg gaaaacaaca
     1801 tggtgcagac ccaagcctcg gctgcgggta cgctttcaac catcatcttc gtcctgcccg
     1861 gtttgctgat ggcgggctac tggagcggtt tcccgttctg gcagacgacg cttttatgta
     1921 ttgccggcgg gattttgggg gtgattttca ccattccgct gcgttacgcg atggtggtga
     1981 aaagcgattt gccttatccg gaaggtgtgg cggctgctga aattttgaaa gtgggcggtc
     2041 atgaagaagg ggataaccgt cagggcggca gcggcatcaa agagctggcg gccggcggtg
     2101 cgttggcggg attgatgagc ttttgcgccg gaggtctgcg tgtgattgcc gatagcgcga
     2161 gttattggtt taaaagcggt acggcaattt tccaactgcc gatgggcttt tcactggcat
     2221 tgttgggcgc gggctatttg gtcggactga cgggcggtat cgccattctg ttgggcattt
     2281 cgattgcttg gggcattgcc gtgccgtatt tttcatcgca catcccgcag ccttccgata
     2341 tagaaatggc ggcgtttgcg atgaagctgt ggaaggaaaa agtacgcttc atcggcgcgg
     2401 gaaccatagg tatcgcggcg gtttggacgc ttttgatgct gctcaagcca atggtggaag
     2461 gcatgaagat gtcgttcaag agttttggcg gcggtgcgcc cgcaacagaa cgcgctgaac
     2521 aggatttgtc gcctaaagcc atgattttct gggtattgtc tatgatgttt attctaggcg
     2581 tgtcgtttta ccactttatc ggcgattcgc acattacggg cggcatggct tggcttttgg
     2641 tggtcgtttg cacgcttttg gcttccgtca tcggcttttt ggtcgccgcc gcctgcggtt
     2701 atatggcggg tttggtcggc tcgtcttcca gcccgatttc cggcgtgggc atcgtttcca
     2761 tcgtcgtgat ttcgctggtt ttgctgctgg tgggcgaatc cggcggtttg atggcggatg
     2821 aggcaaaccg caaatttttg ctggcactga ctttattttg cggctcggca gtaatctgcg
     2881 tggcttcgat ctccaatgac aacctgcaag acctgaaaac cggctacctg ctcaaagcca
     2941 cgccttggcg gcagcaagtc gccctgatta tcggctgtat cgtcggtgcg ctggttattt
     3001 cacccgtgtt ggaactgctt tacgaagcct acggctttac cggcgcaatg ccgcgcgaag
     3061 gtatggacgc ggcgcaggct ttggcagccc ctcaagcgac tttgatgacg accatcgcgt
     3121 cgggcatttt cgcccacaac ctcgaatggg tctatatctt taccggcatc gcgattggcg
     3181 cggtgttgat tgccgccgat ttggtgttga aaaaatcatc aggcggcaaa cttgccctgc
     3241 ccgtccttgc ggtcggtatg ggtatttatc tgccgccgtc cgtcaatatg cccatcgtgg
     3301 caggcgcggt gttggcggcg gtgttgaaac atatcatcgg taaaaaagcg gaaaaccgtg
     3361 aaggccgtct gaaaaacgcc gagcgcatcg gaaccttgtt ctccgccggc ctgattgtcg
     3421 gtgaaagcct gatcggtgtg attatggcgt ttattattgc cttctccgtg accaacggcg
     3481 gctcggatgc gccgctcgcg ttgaatctgc aaaactggga tgccgccgct tcttggttgg
     3541 gtttggcgtt cttcgttacc gggatgtttt tctttgcaca gcgcgtactg aaggcgggta
     3601 ggtagcctgt cggagaaaat gccgtctgaa acgttcagac ggcatttttt atcgggaaag
     3661 tgtaaggcgg agcttttcgg cttgcgccca cgttttgccg gcaaggtctt tgggcgacag
     3721 cagcggcgcg gtttgaagcg gccagcctat gccgattgcc gggtcgttcc atattaaaac
     3781 ctgttcggct tcaggcttgt aatagtccgt gcatttgtag gtaagttcgg tcgtgtcgct
     3841 caaaacgtaa aacccgtgcg cgaagccttc gggtatccaa agctggcatt ggttttgcgc
     3901 ggacagggtt gcacccgccc atttgccgaa agtcggcgaa ccttcgcgca tatcgacggc
     3961 cacgtcgaac acttcgccga caactacgcg tacgagtttg ccttgtgtgt tttcagtttg
//
LOCUS       DAWFIT010000128         3909 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFIT010000128 DAWFIT010000000
VERSION     DAWFIT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.128"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(80..898)
                     /locus_tag="WGQ85_002039"
     CDS             complement(80..898)
                     /locus_tag="WGQ85_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0751374.1"
                     /translation="MKGMNKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(978..2228)
                     /locus_tag="WGQ85_002040"
     CDS             complement(978..2228)
                     /locus_tag="WGQ85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0751375.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGQ85_002041"
     CDS             complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WGQ85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0751376.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2888..3700
                     /locus_tag="WGQ85_002042"
     CDS             2888..3700
                     /locus_tag="WGQ85_002042"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0751377.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          867 a          942 c         1163 g          937 t
ORIGIN      
        1 ccgaaggtaa gggaggggtg tgtccactgt gccgccgcat ttgccgcgtg cggaagatac
       61 ggataacaac cccttggaat taaagggaga agttgcggaa aatatccata tcaggctgtg
      121 ggccgtactt atttacggta ttttcgtatg ccgaaataaa atatttcttg aataaaccgt
      181 aatcgccggt ttcgtaatag gctacgacag cctcggcata ggcgggataa ctgtcggggg
      241 cgaatacgca aggaaaaaaa ccggagcgca tcagcgacaa ggtcatacag tttctggcag
      301 tacgtttatt gcaatcttta aaatattgaa gatacgccaa attattatgg agataaacgg
      361 cgcggtcgaa gggatttcct atttttgggg cttcttgaag caaccatttt aattccgtat
      421 ccaaactttg cgggttggat aaaggcgtgt agtctgtccc gcttatagta acgctgtcgc
      481 ggcggactac gccgccggag cctttttcca gtaagttttc cgaaatcagg ctgtgggtgg
      541 tttttaagaa atccaaccag tcaaacggat cggggcggtc caaaccggac agcaggtgcc
      601 ggtaactttc cctcaggttg attaacatta cggcatccga atataacttc ccgcctgcgg
      661 tttgcccgag tttaagcaat gcctgcgtat cgtattgatt ataagtgttt ccttccaatt
      721 tggcagaact gtacacaaaa tcgataccga tttcttcaat attacgcacg gagtccgtta
      781 aaacagataa aggaagccga tcggacaatt cggtcaggta ttcttgttcg ccggctgtaa
      841 aaatggggcg gatatttaaa aaatcctgtt ggtatctcaa tttattcata cctttcatca
      901 taaaaacgcg gtttggggcg gaagtaggtt tatgcctgcc ttcatccgac aactatattg
      961 aaacaccgcc ttttcaatca accccgcttc aatacggatg tattgaggta gcgttggaca
     1021 cccgaggcaa tggattgggc gcactgccga cggaaggatt cgctgcccag cagcttctct
     1081 tcggcaggat tggacaggaa ggcggtttcg accaggatag acggcatatc gggcgcgcgc
     1141 aaaacggcga aattggcttc gtccaccctg cctttgtgca agtggttgag cctgcccaat
     1201 tcttcaagca ccagtttgcc gagtttgcgg ctgtcgcgca gcgtggcggt ttgggtcatg
     1261 tcgagcaggg cggtatcgac attgcggttg ccgctggtcg gtacgccgcc gaccgcgtcg
     1321 gcattatttt gggtttgttc caagaatttg gcggcagagc tggtcgcgcc tttggtgttc
     1381 agcatataaa cccccgttcc gcgcgcggag gggctggtga aagcgtcggc gtggatggag
     1441 acgaatacgt ccgcccgccg tgcgcgccct ttggcgacac gcacgcccaa tgggatgaac
     1501 acgtcttcgt tgcgcgtcat aaagacgttg taacccaagg cttccaattg ttttttggtt
     1561 tcgcgggcga tggagaggac gacgtgtttt tcctgcaaac cgcccgggct gacggcgccg
     1621 gggtcttcgc cgccgtgtcc ggggtcgatc ataatgacgg gtctgcgccc gtttttgccg
     1681 cgttcggttt gtgcgggagt gttttgagcg aggtcggctt caggggagcc gcgacgggtt
     1741 ttgttcaggc tgccgttgag cagcgccatc atcggatcgt cggcatccat cccttgcgga
     1801 tagagatcga cgacgaggcg gtttttaaag ccgccgacgg gtgggaggga aaaaacttgc
     1861 gcgtaggaag gctgtttcaa atcgatgacg aggcggacgg tgtcgggcgt gttctgaccg
     1921 gcgcgtatgc tgcggataaa ggggtcgtcc gccataactt tttgggaaag tccgtgcaat
     1981 acggtattga tgttcgcgtt ttgtatatcg acgaccagcc tgcccggatt gtcgagcgtg
     2041 aagtgttggt atttgagcgc ggcggtgctt tccagcgtca ggcgggtgta ggtgtgcgac
     2101 ggccatatcc gtacggcggt aaactgcggg gcgcgtaccg ttttggcaac ggcggatgcg
     2161 atggggctta gggcgaacag tgtgccggcg gtgcggcgga tgatttgtct tcgtgtcagt
     2221 ttggtcatag cggcaggctt tcgcgtcctc gttcggtatg ggcggtcagc aggcattttc
     2281 tgccgtcgcc gtcgtgtgtc aatgttgcgg tgatgtcggc aggcggggta aattccccac
     2341 cttgttgcgg ccattcgatc aggcagacgc tgtttgcggc aaacagttcg tccaatccgg
     2401 catcttccca ttcttcgggg aacgagaagc ggtagaggtc gaaatggtgc aaggtgaagc
     2461 gttccagcag ataagattcg acgatggcgt aagtcgggct tttgactgcg ccctgatgac
     2521 ccaatccgcg caggatgccg cgtgtcagcg tggttttgcc cgcacccaaa tccccctcga
     2581 gataaatgac cagcggcgcg tttaaacggg aagaccacgc cgcacccaaa tcgagtgtgg
     2641 cggcttcgtc ggcaaggaat cgggagatag agggtaaatc agacatggaa acggtttgtt
     2701 gtaaggtcta gggtattatg ggcagttttg caggttttgc aaactttgca cccgaggggc
     2761 ggatgcttct tgtccgagca ttataacagc caaatccgcg ttctgctttc agacggcaac
     2821 ggctgtcaag aaaaagcggc gcgtgtacaa tacgcggatt gtatgtttag gacagattgg
     2881 aaaaaggatg gaaaatatcg gcaggcagcg acccatcggc gtttttgact cgggaatcgg
     2941 cggtttgacc aatgtgcgcg cgctgatgga gcggctgccg atggagaaca tcatttattt
     3001 cggcgacacg gcgcgcgtgc cttacgggac gaaatctaag gcgaccatcg aaaatttctc
     3061 gatgcagatt gtcgattttc tgctggaaca cgatgtcaag gcgatggtta tcgcgtgcaa
     3121 cacgattgcg gcggtggcgg ggcagaaaat ccgtcaaaag acgggcaata tgcctgtgtt
     3181 ggacgtgatt tccgccggcg cgaaagccgc gctggcaacg acgcgcaaca ataaaatcgg
     3241 catcatcgcc accaatacca cagttaacag caatgcttat gcgcgcgcca tccacaggga
     3301 caaccccgac acgctcgtcc gcacgcaggc tgcgccgctg ctcgtccctt tggtggaaga
     3361 gggctggttg gaacacgaag ttacccgcct gaccgtatgc gaatacctca aaccattgct
     3421 tgcagacggc atcgatacgc tggtgttggg ctgcacgcac tttcccttgc tcaagccctt
     3481 aatcggcagg gaggcgggca atgtcgcgtt ggttgattcc gcgattacga ctgccgaaga
     3541 aaccgcacgc gtccttgcgc aggagggatt actcaatacc gacaacaaca atcccgacta
     3601 ccgtttttac gtcagcgata ttcctttgaa attcagaacc atcggcgagc gttttctggg
     3661 caggacgatg gagcagattg aaatggtgtc tttgggttaa aacgatgacg gaaagctgcc
     3721 cgagattaca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatct agacctgtcg
     3781 gtgcggaaac ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt
     3841 cgcgggaatg acgggatgca ggtttctgtg cggatgtatt cggattccct cctgcgcggg
     3901 aatgatggg
//
LOCUS       DAWFIT010000129         3861 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFIT010000129 DAWFIT010000000
VERSION     DAWFIT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.129"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGQ85_002043"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGQ85_002043"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0751378.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGQ85_002044"
     CDS             1439..1924
                     /locus_tag="WGQ85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0751379.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGQ85_002045"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGQ85_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0751380.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          782 a          941 c         1080 g         1058 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ttgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgttgg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaagc a
//
LOCUS       DAWFIT010000130         3844 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFIT010000130 DAWFIT010000000
VERSION     DAWFIT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.130"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGQ85_002046"
     CDS             complement(94..531)
                     /gene="rnhA"
                     /locus_tag="WGQ85_002046"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0751381.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            645..1514
                     /gene="tehB"
                     /locus_tag="WGQ85_002047"
     CDS             645..1514
                     /gene="tehB"
                     /locus_tag="WGQ85_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0751382.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGQ85_002048"
     CDS             complement(1564..2370)
                     /gene="thiD"
                     /locus_tag="WGQ85_002048"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0751383.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            2431..2628
                     /locus_tag="WGQ85_002049"
     CDS             2431..2628
                     /locus_tag="WGQ85_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751384.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(3672..3747)
                     /locus_tag="WGQ85_002050"
     tRNA            complement(3672..3747)
                     /locus_tag="WGQ85_002050"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3711..3713),aa:Glu,seq:ttc)
BASE COUNT          855 a          928 c         1113 g          948 t
ORIGIN      
        1 tctggattcc cgcctgcgcg ggaatgacgg tggaggacga tgccgtctga agcccattag
       61 cggtttcaga cggtattttt gtcggtgcgg cagtcaggaa aactgcgccg cgccacggtt
      121 tgccaaatcg tcggcgcgtt cgttttccgc gtgtcccgcg tgtcctttca cccaagtcca
      181 gctgacttgg tgccgtccga ccagcgcgtc gagttctttc cacaagtcgt cgtttttgac
      241 gggctgtttg gcggcggttt tccagccgtt gcgcttccaa ccgtgtatcc agttttccat
      301 gccgtttttg acgtattgcg agtcggtgca gatgatgacg gtgcagcggc gtttgagcga
      361 tttcagtcct tcgatgacgg cggtcagttc catacggttg ttggtggttt gcgcttcgcc
      421 gccgaaaagt tctttttcgt ggctgccgta gcgcattaat acgccccagc cgcccgcgcc
      481 gggattgcct ttgcacgcgc cgtcggtgta aaggtaaacg gtttggttca ttattgtgcc
      541 tttatgtggt gtccggcgtg attataagcg gtttgtgcgc cgtgccgtct gaaagattgc
      601 ggcggattaa aataggcata taatgtgcaa ctttattttt gaatatgaag gagcggatag
      661 tgggtcaaag cggcgagttg ttttgtttcg gacaaatgcc cgtatggaag gcggaaaacc
      721 tgccggaaat tttgttgtcg ggttattcgt ccgaaggagg ggaatgggtc tgcctgaatg
      781 tgttgcaggg cgatgtcgaa gttcgtgcac cggacgggtc ggcagaagtt tggtcggcag
      841 acggcggcga ttgtgtgttt gcgccgcagc aggtgttttc tgtcaaaccg aaaacggacg
      901 atgccgaaat ccgtttgtcg ctgtattgcg cggcggcaga ctattttcac aaaaaatacg
      961 ggatgagtgc cacgcattct gcggtcgcgg cggcacagga taccgtaccg gcgggcaggg
     1021 cgttggatat gggctgcgga caggggcgca acgcgctgtt tcttggtttg aaggggtttg
     1081 aagttactgc agtcgatcac aatcccgccg ccttggcaaa cgtggcggag ctggcagagg
     1141 cggagggttt gaacgtccgc acgctggaat atgatttgaa cgccgccgcc ttgcagggtg
     1201 agtttgatta tattgtggca acagtggtgc tgatgttcct gatgccgcag cgcgtgcctg
     1261 acgtgattgc cgatatgcag gcgcatacgg cggcgggcgg gtacaactta atcgtatcgg
     1321 caatggatac ggcggatttc ccttgtccga tgccgttccc ttttaaattt aaagagggag
     1381 agctgaagga ttattatcgg gattgggagc tggtcgaata taaagaagaa ttgggcgcga
     1441 tgcacgccaa agacgaaaac ggcaatccga tacggtttaa atttgtaacg atgctggcga
     1501 aaaagcctga ataatcctca aaaatgccgt ctgaacattt tgtttcagac ggcattttta
     1561 cggttagtcc cgataggccc aatgattgac cgggccgtgt cccgcgccga tttccaaagg
     1621 gcttgagatt gccgccgtga tgtaggactt ggcagtccgt acggctttgc aaacgtctaa
     1681 gccttttgcc aactcggcgg taatgcaggc agaaaacgtg cagcccgtgc cgtgcgtgtg
     1741 ggcggtcgga aagcgcgggc tgtccaattc cagcgtttcg ttttgcgtaa acagccaatc
     1801 cgtgcagcgt ccgctgctgc tgccattcaa atgtccgcct ttgatgacga catttttgac
     1861 accgtaatca agcaggattt ttgccgcacg ttccgcatct ttacggtttt cgatatgcac
     1921 gccggtcagg gcttccgcct cgggcaggtt gggggtcaat acatccgtat cgggaagcag
     1981 caggcgcgtc agtgccgcaa cggcggaatc ctgcaacagc ggcgcaccgc ctttggcaat
     2041 catcacaggg tcgagtacgc gcctgccgaa gtcgcagtgt ttcagcttgt cggcaacgca
     2101 ttcgatgatt tccgccgtgc cgagcattcc tattttgtag gcgcggatgt caaagtcttc
     2161 cctgattgct tggatttgtg cggtgatggt ttcggtcggg acgagatgaa ccgccgacac
     2221 gcccaaggta ttttgcgcgg taacggcggt gatgacgcac gttccgaaca cgccgcgcat
     2281 ctgaaacgtt ttcaaatccg cctgaatgcc cgcaccgccg cccgaatccg aaccggcgat
     2341 ggtaagcgtt tgcacaaaag agcttttcat ttgttcctcc ttaatattgt gtccgataaa
     2401 aagggctaat gcctgtaaaa aagcacggcg atgccgtctg aaaaccgcag cgcgggggtt
     2461 tgtggtgcat tatatgccgt attgcaggac aaggcacagg gcagggcggc ggggtgcatc
     2521 gtagcaactg tatttttgcc cgacggggtg aaaaatacag ttgctacacc cactgataat
     2581 gaaaaaagag agcaatgccg tctgaaccgc gtgttgcggc gagcataaac cggcacgctt
     2641 tattcgtcat ttccacaaaa acagaaacct aaaattccgt cattcctacg aaagcgggaa
     2701 tccagtgtgt tcggtttcag ttgtttttgg gtttcgggtg atttccaaat catcattccc
     2761 gcgcaggcgg gaatctagcc cttggggcag cggcaatatt tataaagccg tctgaaaatt
     2821 cagaagttct agattcccgt tttcacggga atgacgaagg gttgtgggaa tgacgaagtt
     2881 tcagacggca tcgccccgcc ccgtcattcc cgcataagcg ggaatccaaa ccattgggca
     2941 gagcagcctt aaaggttata aaagactcgt cattcccacg aaagtgggaa tccagacctg
     3001 tcggcacgga aacttatcgg ataaaacggt ttcttcaacc ctacgctcta gattcccgct
     3061 tttgcgggaa tgacgaagtt tcagacggca tcaaatgctt tgtcaggctt cagacagcat
     3121 cgtccaccct ctgccgtcct tcccgcgcag gcgggaatcc aatgtgttcg gtttcggttg
     3181 tttttgggtt tcgggtaatt tccaaatcgt cattcccacg aaagtgggaa tccagaccat
     3241 tgggcggcgg aaatattcaa aagtcgtctg aaaattcaga ggttctagat tcccgttttc
     3301 acgggaatga cgaagggttg tgggaatgac gaagttttag acggcattga atactttgat
     3361 gctttggtgg gctgaagccc accctacaat atagagaaaa tgccgccaaa cggtatggaa
     3421 gcggaaacaa tggtggggct ggcttcagaa acaatggtag ggtgggcttc agcccaccgc
     3481 tttagacggc atccgcgcac cgtcattccc gcgcaggcgg gaatccagcc ttgtcggcac
     3541 ggaaacttat cggataaaac ggtttcttca accctacgct ctagattccc gttttcacgg
     3601 gaatgacgaa gagtgtgaga atgacggttc ataaataaaa aaacctgctt aaaacaagca
     3661 ggtttttgaa ttggcacgcc cacggggatt cgaaccccgg ttgccgccgt gaaagggcag
     3721 tgtcctaggc ctctagacga tgggcgcgtg ggaatgggca ttatacatac ttctagaaat
     3781 ttagcaatac gatgccgtct gaaacttcgt cattcccgcg aaagcgggaa tctagaatct
     3841 ccaa
//
LOCUS       DAWFIT010000131         3830 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFIT010000131 DAWFIT010000000
VERSION     DAWFIT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.131"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            456..3659
                     /gene="nalP"
                     /locus_tag="WGQ85_002051"
     CDS             456..3659
                     /gene="nalP"
                     /locus_tag="WGQ85_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ0751385.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1036 a         1041 c         1023 g          730 t
ORIGIN      
        1 tagattcccg cctgcgcggg aatgacggct gcagatgccc gacggtcttt atagcggatt
       61 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gaaccgattc
      121 acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt
      181 catccactat aactagggaa attcaaatta agttagaatt atccctatga gaaaaagccg
      241 tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt caaatttcca
      301 ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc cctataataa
      361 tataattcaa aatataaaaa cgggttacat ctaaacatta cggaattttt attccctcgc
      421 ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca accttcccta
      481 caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt tctgcctgct
      541 taggcggcgg cggcggcact tctgcgcccg acttcaatgc aggcggcacc ggtatcggca
      601 gcaacagcag ggcaacaaca gcgaaatcag cagcagtatc ttacgccggt atcaagaacg
      661 aaatgtgcaa agacagaagc atgctctgtg ccggtcggga tgacgttgcg gttacagaca
      721 gggatgccaa aatcaatgcc ccccccccga atctgcatac cggagacttt acaaacccaa
      781 atgacgcata caagaatttg atcaacctca aacctgcaat tgaagcaggc tatacaggac
      841 gcggggtaga ggtaggtatc gtcgatacag gcgaatccgt cggcagcata tcctttcccg
      901 aactgtatgg cagaaaagaa cacggctata acgaaaatta caaaaactat acggcgtata
      961 tgcggaagga agcgcctgaa gacggaggcg gtaaagacat tgaagcttct ttcgacgatg
     1021 aggccgttat agagactgaa gcaaagccga cggatatccg ccacgtaaaa gaaatcggac
     1081 acatcgatgt ggtctcccat attattggcg ggcgttccgt ggacggcaga cctgcaggcg
     1141 gtattgcgcc cgatgcgacg ctacacataa tgaatacgca tgatggaacc aagaacgaaa
     1201 taatgtctgc agccatccgc aatgcatggg tcaagctggg cgaacgtggc gtgcgcatcg
     1261 tcaataacag ttttggaaca acatcgaggg caggcactgc cgaccatttc caaatagcca
     1321 attcggagga gcagtaccgc caagcgttgc tcgcctattc cggcggtgat aaaacagacg
     1381 agggtatccg cctgatgcaa cagagcgatt acggcaactt gtcctaccac atccgtaata
     1441 aaaacatgct tttcattttt tcggcaagca atgacgcaca agctcagccc aacacactga
     1501 ccctattgcc attttatgaa aaagatgctc aaaaaggcat tatcacagtc gcaggcgtag
     1561 accgcagtgg agaaaagttc aatggctcca accattgcgg aattactgcc atgtggtgcc
     1621 tatcggcacc ctatgaagca agcgtccgtt tcacccgtac aaacccgatt caaattgccg
     1681 gaacatcctt ttccgcaccc atcgtaaccg gcacggcggc tctgctgctg cagaaatacc
     1741 cgtggatgag caacgacaac ctgcgtacca cgctgctgac aacggctcag gacatcggtg
     1801 cagtcggcgt ggacagcaag ttcggctggg gactgctgga tgcgggtaag gccatgaacg
     1861 gacccgcgtc ctttccgttc ggcgacttta ccgccgatac gaaaggtaca tccgatattg
     1921 cctactcctt ccgtaacgac atttcaggca cgggcggcct gatcaaaaaa ggcggcagcc
     1981 aactgcaact gcacggcaac aacacctata cgggcaaaac cattatcgaa ggcggttcgc
     2041 tggtgttgta cggcaacaac aaatcggata tgcgcgtcga aaccaaaggt gcgctgattt
     2101 ataacggggc ggcatccggc ggtagcctga acagcgacgg cattgtctat ctggcagata
     2161 ccgaccgatc cggcgcaaac gaaaccgtgc acatcaaagg cgatctgcag ctgggcggcg
     2221 aaggtacgct gtacacacgt ttgggcaaac tgctgaaagt ggacggtacg gcgatgaccg
     2281 gcggcaagct gtacatgtcg gcacgcggca aaggggcagg ctatctcaac cgtaccggac
     2341 aacgtgttcc cttcctgagt gccgccaaaa tcgggcggga ttattctttc ttcacaaaca
     2401 tcgaaaccga cggtggtctg ctggcttccc tcgacagcgt cgaaaaaaca gcgggcagtg
     2461 aaggcgacac gctgtcctat tatgtccgtc gcggcaatgc ggcacggact gcttcggcag
     2521 cggcacattc cgcgcccgcc ggtctgaaac acgccgtaga acagggcggc agcaatctgg
     2581 aaaacctgat ggtcgaactg gatgcctccg aatcatccgc aacacccgag acggttgaaa
     2641 ctgcggccgc cgaccgcaca gatatgccgg gcatccgccc ctacggcgca actttccgcg
     2701 cagcggcagc cgtacagcat gcgaatgccg ccgacggtgt acgcatcttc aacagtctcg
     2761 ccgctaccgt ctatgccgac agtaccgccg cccatgccga tatgcaggga cgccggctga
     2821 aagccgtatc ggacgggttg gaccacaacg ctacgggtct gcgcgtcatc gcgcaaaccc
     2881 aacaggacgg tggaacgtgg gaacagggcg gtgttgaagg caaaatgcgc ggcagtaccc
     2941 aaaccgtcgg cattgccgcg aaaaccggcg aaaatacgac agcagccgcc acactgggca
     3001 tgggacacag cacatggagc gaaaacagtg caaatgcaaa aaccgacagc attagtctgt
     3061 ttgcaggcat acggcacgat gcgggcgata tcggctatct caaaggcctg ttctcctacg
     3121 gacgctacaa aaacagcatc agccgcagca ccggtgcgga cgaacatgcg gaaggcagcg
     3181 tcaacggcac gctgatgcag ctgggcgcac tgggcggtgt caacgttccg tttgccgcaa
     3241 cgggagattt gacggtcgaa ggcggtctgc gctacgacct gctcaaacag gatgcattcg
     3301 ccgaaaaagg cagtgctttg ggctggagcg gcaacagcct cactgaaggc acactggtcg
     3361 gactcgcggg tctgaagctg tcgcaaccct tgagcgataa agccgtcctg tttgcaacgg
     3421 cgggcgtgga acgcgacctg aacggacgcg actacacggt aacgggcggc tttaccggcg
     3481 cgactgcagc aaccggcaag acgggggcac gcaatatgcc gcacacccgc ctggttgccg
     3541 gtctgggcgc ggatgtcgaa ttcggcaacg gctggaacgg cttggcacgt tacagctacg
     3601 ccggttccaa acagtacggc aaccacagcg gacgagtcgg cgtaggctac cggttctgac
     3661 ggacaggaag cagacagccg caaagatcac ggtctttgcg gctgtttctt atgaaaagaa
     3721 aaccctattc caattgcctg cttctattgt ttcaagactt cttccaaaga ttcggcatta
     3781 atcagatgta tagcggatta acaaaaatca ggacaaggcg gcgaagccgc
//
LOCUS       DAWFIT010000132         3791 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFIT010000132 DAWFIT010000000
VERSION     DAWFIT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.132"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGQ85_002052"
     CDS             complement(370..1410)
                     /gene="queA"
                     /locus_tag="WGQ85_002052"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0751386.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1527..1784
                     /locus_tag="WGQ85_002053"
     CDS             1527..1784
                     /locus_tag="WGQ85_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0751387.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1766..2221
                     /locus_tag="WGQ85_002054"
     CDS             1766..2221
                     /locus_tag="WGQ85_002054"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0751388.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2333..3694
                     /gene="accC"
                     /locus_tag="WGQ85_002055"
     CDS             2333..3694
                     /gene="accC"
                     /locus_tag="WGQ85_002055"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0751389.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          923 a         1151 c          921 g          796 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa tggcgtatgt
      181 aatatgttta tacgaccaaa acggaatgaa ttttaacgta ttgcgcgtca tcaacaatga
      241 ctgagtttct cgcctctcgc gcctgaatct atgtcatata cagttccttt cattcaaatc
      301 aacaaaagaa tgccgtccga acgtccgttc agacggcact tgtcttcccg caatagactt
      361 ggggctgttc taacgtacca ccccttcgtt ccaccccaaa accatcgcat cgccgtagct
      421 gaagaaacgg tattcgcgtt cgaccgcata acgatacgcg gcgcggatat gacccatacc
      481 cgaaaacgcg ctgacaagca tcagcagcgt cgatttcggc aaatgaaaat tggtaaccag
      541 cctgtcgaca acattaaaac ggtagcccgg cgtgatgaaa atatcggtgt cgccctgccc
      601 cgctttcaga cgacccgtcg cacgcgcggc agattcgagg gcgcgcatgg aagtcgtgcc
      661 gactgcccag actttgttcc cccgggcttt tgccgcctca acggcggcgg cggtttcaga
      721 cggcacttca aaccattcgc tgtgcatttt atgctcttcg attttgtcca cacgcacggg
      781 ttggaatgtc cccgcaccga cgtgtagggt tacttctgcg gttactgcgc ctttgtcttt
      841 caggcggcgc aaaagttctt ccgtaaaatg cagacccgcc gtcggcgcgg cgaccgcgcc
      901 ctgatatttg gcataaacgg tttgataacg gctgtcgtca tccgcatcgg cggcgcgttc
      961 gatataaggc ggcaggggca ggtgtccgtt ctgttccaaa agttcgtaaa cggtttgacc
     1021 gccttcaaaa cgcaggcaga acagttcgcc ctcacgcccg accgtcacgg cgcggatgcc
     1081 gccttcaaac accagcccca taccgggctt gggcgatttg gacgaacgga tgtgcgccag
     1141 tgcggtatgg ttgtccaaca cgcgctcaat cagggcttcg atcctgccgc cgctgtcttt
     1201 ctgcccaaac agccgcgcct tcatgacttt ggtgttgttg aacaccaaaa cgtcgcccgc
     1261 ctcaacataa tccggcaaat cgccgaacac ccggtcttgc agcggcatat cgggcaacgc
     1321 aaccaaaagg cggctgctgc cgcgcacttc gggcggatgc tgggcaatca gcttttcggg
     1381 cagggtaaaa tcaaaatctg aaatatccat ttttacactc tcgttcgggc aagccgccat
     1441 tatacgcact ttagcccttt ttcagacggc atctttgtcc gaaaaaccaa cagattagaa
     1501 taaacactct taacccgggg catcttgtgc gcaaaatcaa acttcctgca catttccccc
     1561 aaaaaccgcc gttttttgat attttactgg acatttaccg acaacttcgg gaaaataaac
     1621 acattctcac ggtcgttttc caccacagga aaaccgtatc cgaacaccat tccgcccggt
     1681 ttgcgccgtt gccgcaagcc ggctgttttc tgaaaaacca acgcaacaac ccgccgaaac
     1741 accggcagcc tttaaaggaa cagaaatgga tttgcgcaaa ttaaaaaaac tgattgattt
     1801 ggttgaagaa tcgggtatcg ccgaaatcga agtaaccgaa ggcgaggaaa aagtccgcat
     1861 cacccgaacc atcgccgccg cacccgttta cgccgcgccc gtacctgccg ccgcgccggc
     1921 cgtaacgcct gccgccgcac ccgttgcggc atccgcgcct gccgccgcac ctgccgcccg
     1981 cgatttgtcc gacgcgcaaa aatcacctat ggtcggcacg ttctaccgcg cacccggccc
     2041 gaatgccgcg ccttttgtcg aagtcggcca acaggttaaa gtcggcgaca cgctgtgcat
     2101 catcgaagcg atgaagctga tgaacgaaat cgaagccgaa aaatccggca cggtcaaaga
     2161 aattttggtc gaaaacggta cgcccgtcga attcggcgaa ccgctcttca tcatcggata
     2221 atcctgtttt cagacggcat aaacttccga tgccgtctga aatgctttcc cccttcagcg
     2281 ttcccgcacc cttttttacg gacgggttgc cggaaccgca ggaaaggtca tcatgctgaa
     2341 aaaagtttta atcgccaacc gaggcgaaat cgcattacgc gtactccgtg cctgccgcga
     2401 aatgggcatt gccaccgtcg ccgtgcattc cgaggccgac aaagacagcc tgcacgtcaa
     2461 actcgccgac gaatccgtgt gcatcggccc tgccgcttcc gcgcaaagct acctcaacgt
     2521 ccccgccatt atcgccgccg ccgaagtaag ctgcgcggac gctgtccacc cgggctacgg
     2581 tttccttgcc gaaaacgccg atttcgccga acaggtcgag cagtccggct ttacctttat
     2641 cggcccgaaa cccgacacca tccgcctgat gggcgacaaa gtatccgcca aacacgccat
     2701 gatcgaagcg ggcgtgcctt gtgttcccgg ctctgacggc gcattgcccg acgacggcga
     2761 agaaatcctc aaaatcgccg ataaagtcgg ttatcctgtc attatcaaag cctctggcgg
     2821 cggcggcggt cgcggtatgc gcgtggtcga gaaaaaagaa gacctcctcc aatctgtcga
     2881 aatgaccaag gcagaagcag gcgcggcatt cggcaacccg atggtttaca tggaacgcta
     2941 tttgcaacgt ccgcgccacg tcgaaatcca agtgattgcc gacgaacacg gcaacgccgt
     3001 ctaccttgcc gaacgcgact gttcgctaca acgccgccac caaaaagtca tcgaggaagc
     3061 accggctccg ttcatcactg aaaaagaacg cgccaaaatc ggcaacgcct gtgccgacgc
     3121 ctgcaaacgc atcggctatc gtggcgcagg tacgtttgaa ttcctgtacg aagacggcga
     3181 atttttcttt atcgagatga atacgcgcgt tcaggttgag catccggtta ccgagctcat
     3241 caccggcgtg gacattgtgc aggagcaact ccgcatcgca tccggcctgc cgctgcaata
     3301 caagcagaaa gacatcaaaa tcgaaggcca cgcgtttgag tgccgtatca acgccgaaga
     3361 cccatacaac ttcatcccca gcccgggccc gattgaaagc tgccacctgc ccggcggctt
     3421 cggtatccgc gtcgacagcc acatttacca aggctaccgc atcccgcctt actacgacag
     3481 cctgatcggc aaaatctgcg tacacggcaa aacgcgtgaa caggcaatgg cgaaaatgcg
     3541 cgtcgcactc gccgagctgg cggtaaccgg catcaaaacc aatacgccgc ttcaccgcga
     3601 cttgttcaac gacccaggtt tccaaaaagg cggcgtcagc atccactatt tggaacactg
     3661 gctggaagat cgcaaagcca aacaggacaa ataagccgcc gccgatatgc cgtctgaaac
     3721 agtttgtccg ttttcagacg gcatttccct tgccccgcgc cgtctgaaac cgatttcgat
     3781 atagtggatt a
//
LOCUS       DAWFIT010000133         3584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFIT010000133 DAWFIT010000000
VERSION     DAWFIT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.133"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="WGQ85_002056"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="WGQ85_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0751390.1"
                     /translation="MPYQQITINVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="WGQ85_002057"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="WGQ85_002057"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0751391.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(1909..3192)
                     /gene="hemL"
                     /locus_tag="WGQ85_002058"
     CDS             complement(1909..3192)
                     /gene="hemL"
                     /locus_tag="WGQ85_002058"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0751392.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(3256..3429)
                     /locus_tag="WGQ85_002059"
     CDS             complement(3256..3429)
                     /locus_tag="WGQ85_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0751393.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          881 a         1057 c          927 g          719 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccatcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaatcgc cgcacaagaa tgcgggttaa aagacttggc
      301 atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt ctcacgcaat cgcaattcga
      361 ccccatccga atttccgacc gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcactggca gccacccaac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgctgccctc aaactcggcg caggttctgc
      601 cgtcggcgtc gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacaaaa
      661 caacgtcgat gcacaattct tcctgcccga cggcctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtattggcgc aatccgaagt ttacgccgtc
     1081 caccaaagcg acgagctgct cgacaatatg gacgaatgga acaccgccac acacggtagg
     1141 acggggctga cacagcgcgt gcgcgaatcg ttgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc tcgacgtttc cacgctcaaa gagcttgcca aacgctggaa tccgtccgtt
     1381 gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa aaaatgccgt ctgaagcgca aatcctgcat ttcagacggc atttttacag
     1561 cagattgaaa tcaaaaatat acacgcccgt cattcccgcg caggcgggaa tccggaaggt
     1621 cgggcctgcc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     1681 gggaatgacg ggcgtgtgca ttcttatagt ggattaacaa aaatcaggac aaggcgacga
     1741 agccgcaaac agtacaaata gtacgaaacc gattcacttg gtgcttcagc accttagaga
     1801 atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactatac
     1861 ttcaatctgc caaacagatc gaacagagaa actctgtccg tcaaaacatc atgcagccat
     1921 cgccttgaac acttcaaccg caaccgcaac cgtttcgtca atcagctcgg gcgtatgcgc
     1981 ggcagaaacg aaacctgctt cataagcgga cgggccgaag gcaatgccgc ggtcgagcat
     2041 accgtggaag aagcgtttga aagcgtcaat attggaacgc gccatatcgg cataatttcg
     2101 cggcgcgtgt gcggcgaaat acagaccgaa cataccgccc acgctgtcgg cggtgaactc
     2161 gatgcccgcc gcatccgctg ccgtccgaaa accttgaacc aactgttcgg tacgcgccgt
     2221 caggttttca taaaaccctt cgcggcggat gatttccagc gttttcaaac cggcggcgac
     2281 ggcaatcggg ttgcccgaca aggtgcctgc ctgatacacg ccgcccagcg gggaaataca
     2341 ttccataatg tctttgcgtc cgccgaacgc ggcaagtggc ataccgccgc cgatgacttt
     2401 gcccatcgtg gtcaggtcgg gcgtgatgcc gtgcagcgat tgtgcgccgc cgagcgcgac
     2461 gcggaaaccg gtcatcactt cgtcgtaaat caacactgcg ccgtgttttt cggtcaattc
     2521 gcgcagggct ttgacgaagg cttcggtcgg gcggacaagg ttcatattgc cgacaaaagg
     2581 ctccaaaatc acacaggcaa tgtcattgcc gctttgagca aaggcttctt ccaattgggc
     2641 gatgttgttg tactcaagga ctaaagtgtg tttggtaaag tcggcaggca caccggcgga
     2701 agaaggattg ccgaacgtca gcaggccgct gccggctttc accagcaggc tgtcggaatg
     2761 cccgtggtag cagccttcaa acttgatgat tttgtcgcga ccggtaaaac cgcgtgccaa
     2821 acggatggcg gtcatcgtcg cttcggtacc ggagctgacg aggcgcagcc gttcgacgga
     2881 cggcatgatt ttggcgattt gttcggcaat ggcgatttcg ccctcggtgg gcgcgccgaa
     2941 cgacaaaccg cccaacgcgg cttcgcgcac ggcttcgacg acttcggggt gcgcgtgtcc
     3001 gacaatcgca ggcccccaag agccgacata gtcggtataa cgcgtgccgt tttcgtccca
     3061 aacatacgcg ccttcggctt ttttgatgaa gcgcggcacg ccgccgacgc tgccgaatgc
     3121 gcggacgggg gaattcacgc cgccggggat gatggctttg gcgcggtcga ataaaatttc
     3181 gttacggttc atatttatcc tcaaatgccg tctgaacggc aggtttcggg cttagaagca
     3241 gaaagtccca ttttatcatt tttcaggttg cgacaaggat ttgcctgctt ctttgcggat
     3301 gacgccaacc gcatcccgga tgacggaacg ctcgtctttt tccactttat gtgtaaagcg
     3361 gtagtcccgg acaactcctt cccagtcgta atccacacac cactcccaat gccggcgttc
     3421 tgatttcata taaatgaaat tggtcggcaa aaaattataa atcggcaggc tgacttcatg
     3481 ataggcataa caaccgaaag ggttgcgctt cccgaaacgt gcctctacct ccgcccgggt
     3541 cgttttgcct ttaacaaccg tttgtgcgat tccctcttcc gtct
//
LOCUS       DAWFIT010000134         3457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFIT010000134 DAWFIT010000000
VERSION     DAWFIT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.134"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="WGQ85_002060"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="WGQ85_002060"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0751394.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1990..3384
                     /locus_tag="WGQ85_002061"
     CDS             1990..3384
                     /locus_tag="WGQ85_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0751395.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          897 a         1058 c          867 g          635 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt cgaactgttg
      301 gaagacatga tccgcgtcgg cggtctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatc gtgcgcgagg cggcaaaacg caccggacag
      421 gaaatcgcca tcattgccga cctgcagggc ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcatcg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt tgccgcaggc
      601 gatgtcttgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccac gtcctgaaaa gcaacaaggg catcaacaaa
      721 cgcggcggcg gtctgtccgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctatctcgcc atcagctttg tgaaatccgc cgaagatttg
      841 cacatcgcgc gcgccaaagt cgaagaagaa atgaaaggca gtacggccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtagaagcg attgaaaacc ttgatgaaat catcctcgcc
      961 ggcgacggca ttatggttgc acgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtgc cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggcacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcga ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggtcg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggtgcggtc agcgtggcgc gcgcggttca cgccaaagcc
     1381 atcgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgcc cgctgatttt ggcaaccagc accgaccacg acacggcgtt aaacgaagtc
     1561 gaaaccatgc tggtggaaca caacatcctg cattccggcg accaatacat cattaccagc
     1621 ggctcgcaaa tgcgcgaatc cggttcgacc aatacgctgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag tcgatgcccg aggcttcaga cggcattttt
     1741 tgcggcggcg cggcggttcg ggcaaactct gaaaaaattg tgcaaatccg gcaacatcgg
     1801 ataaaatcga gtacctatac taaagcgaaa caaggcattt ccgactgcct tttttatagt
     1861 ggattaaatt taaaccagta cggcgttgcc ttgccgtact atctgtactg tctgcggctt
     1921 cgtcgccttg tcctgatttt tgttaatcca ctatatttat ccaccgtccg cctttttacg
     1981 gaaaccgaaa tgaccccttc cgcactgaaa aaaaccgtcc tactgctcgg cactgccttt
     2041 gccgccgcat ccgcacaagc ctccggctac cacttcggca cacagtcggt caacgcgcaa
     2101 agcacggcaa atgccgccgc cgcagaagcc gccgacgcat cgaccatctt ctacaaccct
     2161 gccggcctga ccaaactcga cagcagccag atttccgtca acgccaacat cgtgctgccc
     2221 agcattcatt atgaggcgga ttccgccacc gactttaccg ggcttcccgt ccaaggttcg
     2281 aaaagcggca aaatcaccaa aaccacggtc gcgccccaca tctacggcgc atacaaagtc
     2341 aacgacaatc tgaccgtagg cttgggcgtg tacgtcccct tcggttctgc caccgaatac
     2401 gaaaaagatt ccgtgttgcg ccacaacatc aacaaactcg gtctgaccag catcgccgtc
     2461 gaacctgtcg ccgcgtggaa actcaacgaa cgccattcct tcggcgcagg catcatcgcc
     2521 caacatactt ccgccgagct gcgcaaatat gccgactggg ggattatgga aaaagcgaaa
     2581 gcactaaaag aaacaccccc caatccaact aaagccgccc aaatcaaagc cgacggacac
     2641 gccgatgtca aaggcagcga ttggggcttc ggctaccaac tggcgtggat gtgggacatc
     2701 aacgaccgtg cgcgcgtggg cgtgaactac cgttccaaag tctcacacac gctcaaaggc
     2761 gatgccgaat gggcggcaga cgacgcaatg gcgaaacagt tatgggatgc aaacaaactc
     2821 gcactgctcg gctacacgcc aagcgaaaaa gcccgcgtta aaatcgttac gcccgagtct
     2881 ttgtccgtac acggtatgta caaagtgtcc gacaaagccg acctgttcgg cgacgtaact
     2941 tggacgcgcc acagccgctt cgataaggcg gaactggttt ttgaaaaaga aaaaaccatc
     3001 gtcaacggca aatccgaccg caccaccatc acccccaact ggcgcaacac ctacaaagtc
     3061 ggcttcggcg gttcttatca aatcagcgaa ccgctgcaac tgcgcgccgg catcgctttt
     3121 gacaaatcgc ccgtccgcaa cgccgactac cgcatgaaca gcctgcccga cggcaaccgc
     3181 atctggttct ccgccggcat gaaataccat atcggcaaaa accacgtcgt cgatgccgcc
     3241 tacacccaca tccacatcaa cgacaccagc taccgcacgg cgaaggcaag cggcaacgat
     3301 gtggacagca aaggcgcgtc ttccgcacgt ttcaaaaacc acgccgacat catcggcctg
     3361 caatacacct acaaattcaa ataaacggcc cgccgtttga ttgtagaaat gccgtctgaa
     3421 acagatttcc gtttcagacg gcattgtttt aggcgcg
//
LOCUS       DAWFIT010000135         3406 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFIT010000135 DAWFIT010000000
VERSION     DAWFIT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.135"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(54..527)
                     /locus_tag="WGQ85_002062"
     CDS             complement(54..527)
                     /locus_tag="WGQ85_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0751396.1"
                     /translation="MEQKGFTLIEVMIVVTILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
     gene            complement(532..1128)
                     /locus_tag="WGQ85_002063"
     CDS             complement(532..1128)
                     /locus_tag="WGQ85_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0751397.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNTGNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMKYKKGTRSVSKMPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            complement(1107..2045)
                     /locus_tag="WGQ85_002064"
     CDS             complement(1107..2045)
                     /locus_tag="WGQ85_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0751398.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVISDTTQQNSP
                     FSLKRNGIDKLIPIAESSNINYQNFFQVGSALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGKIADEEGLFRFQLDDKGK
                     WGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSSTNAVTPAGVEVLL
                     SSGIDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            complement(2042..2662)
                     /gene="pilV"
                     /locus_tag="WGQ85_002065"
     CDS             complement(2042..2662)
                     /gene="pilV"
                     /locus_tag="WGQ85_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0751399.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLPHTLSAVDGDFA
                     IDAMKTKGQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTLSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            complement(2686..3354)
                     /locus_tag="WGQ85_002066"
     CDS             complement(2686..3354)
                     /locus_tag="WGQ85_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0751400.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTEDVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPRGDRRTM
                     CQYK"
BASE COUNT          893 a          854 c          682 g          977 t
ORIGIN      
        1 ccataaaaat gccgtctgaa cattgatttt cagacggcat tggcaaaaca gccctatttt
       61 ttacgattag agaaagcttc acagccggca tccgcggaca aagtctccga ataggctcgg
      121 gcagaagcgg catcacggca tttgtatccg tcgcccacgc tgttcatcca taccgacaaa
      181 gtataacccg tccccgtctt tggaacgccg accaagctgt atgcccttgg tttttcctta
      241 ttgacaaaat gcaccgaaac attatatttt tcggcaattt tcggattcat cttatagcct
      301 gagacaaata tttccagttt gctcttgatg gtctgattat cgtccagggg atttttcaaa
      361 ataaactgtt tggaaatatt gttgataccg accatctccg tataaagctg ggactgatag
      421 cctttttcaa tataactttg ataagaaggt atggcaatga cgctgataat gccgagtatc
      481 gtgacaacta tcattacctc aatcaatgta aacccttttt gttccatttt attactcatc
      541 attattgctt acataagatt gaaggacgac cacggtattg gcattcttac cccaagcctt
      601 ggcagtaacc cgataaacat tttctccgtt cttcacgccc aaatattcga taatataacg
      661 tggcattttg ctgacgcttc tcgtgccttt cttatatttc atccctttct tgtcaatgca
      721 caggtcggta gaatttgcag ggcaagaacg cttcaccgcc tcaacggtgg gcttgccttg
      781 caccacgata ttgtcaaaag cctcttcatt accagtatta tttgtccgca cattcactgc
      841 ggtacacaga ccttttccac agttttcgct aaatgtaacc ttactgtccg tatcatattc
      901 caaatccaaa acctgaagtt cgccttcccg caaagccgcc tcggccaaag acaaagccaa
      961 tttcctgtct gattcgttgg cactgatccg ctgctcggta ttgtaagact gcgcggcagt
     1021 tacaaccaaa aaagccacga cgatcatcac catcagcacg ataaacagtg caaaccccct
     1081 ctgtccgtca gaagtcggga ttcccgtcaa agtgttctgt ttgcgcatac atttcccccg
     1141 cgtattgtcg catcgatacg gtaagcataa atatgattgt ctgaagaagc ggcaatcttg
     1201 gtatcaatac cgctactcaa taaaacctcc accccggcgg gcgtaacagc atttgtggag
     1261 ctgtcgaatt tatccgtata tttgaatgtt tcctctttgc cggcatcgtc atcttcagga
     1321 cagccggaaa catagatata ccgcactttc atatgtctaa cctttttcac gagcaactga
     1381 ggattacccc acttgccctt atcatccaat tggaagcgga acaaaccttc ctcatcggca
     1441 atcttgccga ccgcataggc attgaccaca tgcctttgac gggtgatatt tccattttgt
     1501 ttatcatcat tagtaatctg taatgcactc tttgcttctt gcaaggtaga tattttctta
     1561 cccggttttg ctattttggc acagctgctg acgacggtag tatcggcgct tgcattaaca
     1621 tcatcgattc cgtattgaaa aatcaatgcg ctaccaacct ggaaaaaatt ctgataattg
     1681 atatttgaag attccgctat gggaataagt ttatctatac cgttcctttt taaggaaaaa
     1741 ggagaatttt gttgcgtcgt atcggaaata acatcagttg caggatgctc ggacatattg
     1801 aaacaaccga agccgcctgc cattctcgca tcgcggacaa tcaatgttgc cgcattccgc
     1861 aaatcctgtt gcgcggcaag acgctcgttt gccgcatcat ttaatttccg ggatgtgaag
     1921 taactcgatc cgaccgccat caggacaatc atactgagca ggcccgcaac caaaaattca
     1981 ataatggtaa aacctttcat accatcataa ctgccttttg gtacgtttag cattttacgt
     2041 ctcattcccg acctccgacc cttgcctgat aagtatatac gatattgtcg ccgctcacct
     2101 caagattcgt acgggaaata tccgaatccc ctgccgaatc atttacccac aatactttaa
     2161 ttaaagtatc cccgtttgcc ttattgtcgc aatttgaaga aaaagcattg ccggacaatg
     2221 tcggcgcgtt acccgacgaa tccttgcaga cggcgtaatg gatggctgcc gcatccggca
     2281 aggcattttt cagctcataa ctaaatctct tcaattgtgc ctctgccaat tgccccttag
     2341 ttttcatggc atcaatcgca aaatcgccat ccacagctga tagtgtatgc ggtagtgtat
     2401 ggtttcccat gtaaagatta tagtttttct tgttgctgtc cgaatcaatg gtcggattca
     2461 tcaacattcc ctccatcagg ttttgcgtga tttggctgac gatggtttgt gtctccgcct
     2521 ccctgacgga agcgactgtc cgcaactgta cagacaatag tgccaaaata ccgatggtca
     2581 gaacgagcat agcaaccaag acttctatca gcgccatacc ggactgggaa tctttcaggc
     2641 ggaagcaatc attattcttc atattcattt ttaaaactga aactgttatt tatactggca
     2701 catagtacgc cgatcacccc tagggcaaac ttcgaccctg ccgctgctgt taatcaaaac
     2761 caccgccgaa cggaatttct tttcatcggc agaaaccgcc ttcgcatctg tcaacacgat
     2821 ttggatataa ccgtcagaat aaaaaaagct ggattgtttt gtaagatgct ggtctttcgt
     2881 ataaccgaac gtcccgtttt gattgaatgt ccaaactaca cggtcggtgg tcggctgagt
     2941 ctgaccgaaa gcgatatggt tgaaggcata attaatccgc ttatcattga tatcatcatt
     3001 caataccaca ctgcggagaa gaacatcctc cgtatcattg tcatatccct tattgccgtt
     3061 tttgtcgccg aaagccaaca ttccctgccc cttcttgccg gagtcacatt tattgttggg
     3121 cgtaccgtct tttttaactt gaacaggaca gatatagaca gggagattga gccggacggc
     3181 ttcgcccctg gagaaacgca aaaggttggc aacctgctcc gcgtgactgg caatgcggcg
     3241 ggatgcaatc cattggctca tattggggag ggctatcatc gccataatgg ctgcaatgac
     3301 catcacgatg agcagctctg ttagcgtgaa accttgttgt tttcgtgtac acataggacg
     3361 ttaactggta atgtatcgtg gattaaattc aaaccagtac ggcgtt
//
LOCUS       DAWFIT010000136         3294 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFIT010000136 DAWFIT010000000
VERSION     DAWFIT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.136"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(82..2253)
                     /locus_tag="WGQ85_002067"
     CDS             complement(82..2253)
                     /locus_tag="WGQ85_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0751401.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIGR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSRTGYAGNVKGPNH
                     TQITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGKFKISDKKPDPNDPTKE
                     IDKDAAEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          736 a          748 c          899 g          911 t
ORIGIN      
        1 tgttaatcca ctatatatca aattatcaga acagatgccg tctgaaaggc tttcagacgg
       61 cattttttcg ggatgtgcgt tttagaactt gtagttcacg cccaagcgta catcgcggcc
      121 cacgcccggc agggtattgg tccagcgttg gctgtgcgga tagtagaacg tgttgaacac
      181 gttgttaacc gaaagattaa cattgagcgt gtctttgccc agcggtttcc agttggcgaa
      241 gacatcgttc acaccgaaac cttggcgtac aacgttttcc aatttgccgc tgcggtcttt
      301 ttgacctgcc accaatatcg aacccacggc tttttgaaca tagcgtccgc gccagccgat
      361 ttccagattc ggattttgga agcggtaggc aagggaggcc gtccaagtgc ggccgacttg
      421 tgcgccaaat tcaggattcg cgctcaacag cttgtctttg tgcgtatcgt aaaagcgcgg
      481 tttgctgtgg cttacgccga ctttggcagt caggccgccg gtgcggtagg acgcgcccaa
      541 ttcgtaaccg tggtttttga tgtaaccggc gttgacggct tcacggacgg cgacagagtc
      601 gtggcggttt tgcggattgg caagcgcgtc tttgatggtt tgccagaagt agctgccgtt
      661 ggcggcaaac gtgccgtcgt tgtagttgaa gccgatttcg gtattgcgcg cgcgttcggc
      721 tttcgtgccg tcggcaatcg agatgatgcc gcgtttaccg tgggtttgca gcgcgtcata
      781 caggcgcggg ctgcggctgg cgtagttgtg gctcgcgctg aagctccagt gttcgtgcgg
      841 ctgccaaatc acaccgaaac tcgggttaag gttgctgctt gaaacggttt tgccgtcgtg
      901 ggttttcact ttgaagcggt cgtaacgcag cccgccggtc agggtaaagc cgtcaatctc
      961 gtgaatggct tcgatatacg cgccggtatc ggttttggtc ggattggaca gtttgtagga
     1021 atggacaaga tccatatctt tcttgtcttt cgctttttct gcagcgtcct tatctatttc
     1081 ttttgtggga tcgtttgggt ctggtttctt atcggagatc ttaaatttgc cgttcaaaaa
     1141 cgcttgcggt ttgatttcct gatggcggta gttgataccg tatttcagca gggtttgttc
     1201 ggcaaggcgg ctgtcgaagt tgaagttcat accccgagtg gtgatttggg tatggttggg
     1261 gccttttaca ttgcctgcgt agccggtgcg gctgtcatcg gcggaatagc gttctttttc
     1321 caacacatag gcgttggcat ccagtttttc gacaaagccc aggtttttac ccgtgtacgc
     1381 caagttggtg ttggattggg tagtttcgcg gtaagcaggg gcttggcggc caatatttat
     1441 ccgtgaactt ttgtcgccga cggtaaattc ttcacgcaca gtgcggatgc ctcggtgttg
     1501 gtctttcata tggctcaaca cgatgcggtg gtcgccgtcg ccgaagcttg ttccgatttt
     1561 ggcgaggtag ctgcgtttgt ccagcgcgct gtacggtacg gttttgccgc cgttaacatt
     1621 gcggaaacct ttgccggctt cgtaatcttt ttcatcgttg cggttgtaag agaacaaacc
     1681 gtcgaagttg ccctcttttc cgaatacgct tgcgccgtag cttacgcctt cgttgctggc
     1741 aaagccgctg ttgaggcgca cgccccagtt tttatccaag cctttgagca ggtcttgggc
     1801 atcgacggtt ttggcgataa tcgcgccgtt ggtcgcgccg ataccggcag aggcggaacc
     1861 cgcgcctttt tgtacggaaa cgactttaac caaagcggga tcgacaataa atctgccttg
     1921 gtggtaaagg atttggctgt cggaataggc gttgtccacc ttgatgtcga cagagttctg
     1981 acccataccg cgcagcgtca ggaattggga cgtgccgttg ccgccgccga aatcgatgga
     2041 cggctcttct tttaagagtt cgcgcatatc ggttgcggtg ctttcgtctt tttgttgaag
     2101 cgtaacgatg ttggtacgga ttttgctgcc ttggcggtcg ccttttacgg taacggtatc
     2161 caatgcgaca ttggcattat tttctgccgc atgggcaaaa cctgccgcca gggtaagcga
     2221 gagcaggctg aggcggaaaa acggggcgtt catttgttcg tccttttgag tgtatgaagg
     2281 gaagtaaatc caaaccgtta agatttggca ggataagaaa aataattaat aattattttt
     2341 atttatatta acaggggggg ggggattttt gcaaagctga ttatcgtttt tatttgcatt
     2401 gtgttgtttt tgttgacaga ttttgtcgga aatgtaaaaa acggcgggaa tatagtggat
     2461 taacaaatgc gggaatgacg aagcctgcgc gggaatgacg aaaagcaacc tttccgcgtc
     2521 attcccgcga aagcgggaat ctaggaactc aaagctgcaa gaatttatca gaaacaactg
     2581 gaactcaaaa aaactagatt cccgcctgcg cgggaatgac gaagggttaa agaaatgatg
     2641 agaaacggcg gtaatttatc gggaataacc gaaaacgaac ggaaatcagg acaaggcgac
     2701 gaagccgcag acagtacaaa taggacggaa ccgattcact tggtgcttga gcaccttaga
     2761 gaatcgttct ctttgagcta aggcgaggca acgacgtact ggtttttgtt catccactat
     2821 aacagcaacc ctgtcgccgt cattcccgcg aaagcgggaa tccagtccgt tcagtttcgg
     2881 tcatttccga taaattcctg ctgcttttca tttctagatt cccacttttg tgggaatgac
     2941 ggcggaaggg ttttggtttt ttccgataaa ttcttgaggc attgaaattc tagattcccg
     3001 cctgcgcggg aatgacggcg gagcggtttc tgttttttcc gataaattcc taaaacttaa
     3061 aatttcatca ttccctcaaa aacagaaaac caaaaacaga aacctaaaat tcgtcattcc
     3121 cacgaaagtg ggaatccaga acgtaaaatc tgaagaaacc gttttatccg ataagtttcc
     3181 gcaccgacag acctagattc ccgcctgcgc gggaatgacg gcggagcggt ttctgttttt
     3241 tccgataaat tcctaaaact taaaatttca tcattcccgc aaggacagaa aacc
//
LOCUS       DAWFIT010000137         3256 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFIT010000137 DAWFIT010000000
VERSION     DAWFIT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.137"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            584..907
                     /locus_tag="WGQ85_002068"
     CDS             584..907
                     /locus_tag="WGQ85_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0751402.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            1021..3198
                     /locus_tag="WGQ85_002069"
     CDS             1021..3198
                     /locus_tag="WGQ85_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0751403.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTISQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          803 a         1113 c          819 g          521 t
ORIGIN      
        1 cgataacgct ttcacaaagc ctgtgtgcac tgaaaactga aaggtcatct gaaaacggag
       61 ctttcgaagg aagcagagtt tctgcgaagc taaaaccgaa gctgcaaaaa atcgaaaagc
      121 agcctgcacg ttgaaaatgc cgcccaagca aacctgcggt ttgccgccct ctccctagcc
      181 ctctcccaca gggagagggg attggggtgc aggctgtctt taaggttcag gcaaattttt
      241 aacttcgttg aagctgcgat ttcagaagct ccgttttagc ttcgcagaaa ctccgcttcc
      301 ttcgaaagct ccgttttcag acgacctttt ggaacaccac aggcacacgc atcgaacggc
      361 tgaatcaaag attcagaccg atggcagtcc gcacccgagt ttatgcggca aacagcgagg
      421 ctacggcaac ccgtcccctc tccctgtggg agagggttag ggagagggcg gtaagccgca
      481 ggcttacatc aaagccgata acgcttccgt tacaactccg cccactgaac gaagccaaaa
      541 cgacaaaccg catcgtaaac cacccaaccc ataggagaac cccatgcaaa acgaaaccat
      601 caacctgaaa cagcaccttg ccgccatcaa agaatactgg cagcccgaaa tcatcaaccg
      661 ccacgggttc caattccgtt tggtcaaact tttgggcgat tacggctggc atacgcacgg
      721 atacagcgac aaagtgctgt ttgccgtgga gggcgacatg gcggtggact tcgccgacgg
      781 cggcagcatg acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg tgtcgcaccg
      841 cccgcgttcg gaaaacggct gctcgttggt gctgattgag ttgtccgacc cgtccgaggc
      901 cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa gcagcctgca
      961 ccttcaatca atatgccgaa aatacaaccc caccgcacac caacacacaa aggaaatccc
     1021 atgacacgct tcaaatattc cctgctgttt gccgccctgt tgcccgtgta cgcgcaggcc
     1081 gatgtttctg tttcagacga ccccaaaccg caggaaagca ctgaattgcc gaccatcacc
     1141 gttaccgccg accgcaccgc gagttccaac gacggctaca ctgtttccgg cacgcacacc
     1201 ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt catcacatcg
     1261 caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca ggcgaccggc
     1321 accagccgcc agatttacgg ctccgaccgc gcgggctaca actacctgtt cgcgcgcggc
     1381 agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct ggccgatacg
     1441 ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt ggcggggctg
     1501 ctggacggca cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg cccgacccgc
     1561 aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg gctggacgcg
     1621 gacgtatcgg gcagcctgaa caccgaaggc acgctgcgcg gccgcctggt ttccaccttc
     1681 ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact ctacggcatt
     1741 ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta ccagcaggcg
     1801 aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg acagccaagg ttatgccacc
     1861 gccttcggcc cgaaagacaa ccccgccaca aattgggcga acagccgcca ccgtgcgctc
     1921 aacctgtttg ccggcatcga acaccgcttc aaccaagact ggaaactcaa agccgaatac
     1981 gactacaccc gcagccgctt ccgccagccc tacggcgtag caggcgtgct ttccatcgac
     2041 cacaacaccg ccgccaccga cctgattccc ggttattggc acgccgaccc gcgcacccac
     2101 agcgccagcg tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca cgatttaatc
     2161 gcgggtatca acggttacaa atacgccagc aacaaatacg gcgaacgcag catcatcccc
     2221 aacgccattc ccaacgccta cgaattttcc cgcacgggcg cctatccgca gcctgcatcg
     2281 tttgcccaaa ccatctcgca atacggcacc aggcggcaaa tcggcggcta tctcgccacc
     2341 cgtttccgcg ccgccgacaa cctttcgttg atactcggcg gcagatacag ccgttaccgc
     2401 accggcagct acgacagccg cacacaaggc atgacctatg tgtccgccaa ccgtttcacc
     2461 ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt cgctttacgg ctcgtacagc
     2521 agcctgttcg tcccgcaatc gcaaaaagac gaacacggca gctacctgaa acccgtaacc
     2581 ggcaacaatc tggaagccgg catcaaaggc gaatggcttg aaggccgtct gaacgcatcc
     2641 gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg cgacctgagc
     2701 ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga aatcgaagtc
     2761 ggcggccgca tcactcccga atggcagata caggcaggtt acagccaaag caaaacccgc
     2821 gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt caaactcttc
     2881 actgcctacc actttgcccc cgaagccccc agcggctgga ccatcggcgc aggcgtgcgc
     2941 tggcagagcg aaacccacac cgaccctgcc acgctccgca tccccaaccc cgccgccaaa
     3001 gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat catggcgcgt
     3061 taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg acaacctgtt caacaaacac
     3121 taccgcaccc agcctgaccg ccacagttac ggcgcactgc ggacagtgaa cgcggcgttt
     3181 acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaagcta tagtggatta
     3241 acaaaaacca gtacgg
//
LOCUS       DAWFIT010000138         3213 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFIT010000138 DAWFIT010000000
VERSION     DAWFIT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.138"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(405..881)
                     /gene="greA"
                     /locus_tag="WGQ85_002070"
     CDS             complement(405..881)
                     /gene="greA"
                     /locus_tag="WGQ85_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0751404.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1273..2574
                     /gene="aroA"
                     /locus_tag="WGQ85_002071"
     CDS             1273..2574
                     /gene="aroA"
                     /locus_tag="WGQ85_002071"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0751405.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2617..3150)
                     /locus_tag="WGQ85_002072"
     CDS             complement(2617..3150)
                     /locus_tag="WGQ85_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0751406.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          669 a          862 c          874 g          808 t
ORIGIN      
        1 tgcgcgggaa tgacgaattt taggtttctg attttggttt tctgtttttg aggaatgacg
       61 ggatgtaggt tttcttaacc ctgcgtccta gattcccgct ttcgtggtaa tgacgggatg
      121 tgggttcgtg ggaatgacgc ggtgcaggtt ttcttaaccc tgcgtcctag attcccgctt
      181 ttgcgggaat gacggcgaca gggttgctgt tatagtggat gaacaaaaac cggtacggcg
      241 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctcaagcacc aagtgaatcg
      301 gttccgtact atctgtactg tctgcggctt cgtcgccttg tcctgatttt tattaatcca
      361 ctataatttc ctgcgtgtgt cgggtgtatc gaaatcaagc cgaatcaaat atatcggact
      421 tcgataatgt cgtattcgcg cacgccgccc ggggcttgga cttccgccgt atcgccctct
      481 tccttgccga ttaaggcgcg ggcaatcggt gagccgacat agattttgcc ctgtttgata
      541 tccgcttcgt cttcgccgac aatttggtag gtaacgtgtt cttccgtttc caaatcttcc
      601 agcgtaaccg tcgtaccgaa cacgattttg ccttcggcgt ggatttcggt cggattgatg
      661 atgtgggcaa cggaaagttt gtgttccagc tcggaaatgc ggccttcgat aaagccttgg
      721 cgttctttgg cggcttcata ttcggcgttt tcggacaaat cgccgtgcga tctggcttca
      781 gcaatcgctt cgatcacttc ggggcgcgcc acgcttttaa gctgctgcaa ttcctgtttc
      841 agcaattccg caccgcgtac ggtcaggggg attttttgca tcggtctttt ttctccatat
      901 tccggcacac cggtttgcgg cagcaaacat accgcatacc gtcttgtttt gtgcgtccgg
      961 atattaaaat aaaaacacaa gccgcccgga aaatcggcgg cttgtctgtc gttgaacagc
     1021 ggctattcta ccaaattcta tgaaattggc aatcgtgccg tgccgtccgc aaacacgcta
     1081 tgtccgcaac aaaagccgaa aatatgccgg caaaaaaatt ttagaaacaa aaaatttaaa
     1141 aataatcaat tttcggcaca aaaaaccata ttcacggact ttaaaaccga aaatgccaaa
     1201 cctgagattt ttcagacggc atttgcacca gtataatgca ggctgttttt atctttatat
     1261 ttacgttttg ccatgaccga atccctccgc ctccctgccg cctcgctcaa accttccact
     1321 gtcgccctgc ccggctccaa aagcatcagc aaccgcaccc tgctgcttgc cgccttgtcc
     1381 gacaatgctt gcgaaatcca ttccctgctc aaatccgacg ataccgaccg tatgctcgaa
     1441 gcactcgata aactcggcgt tgaaatcgaa tatcttgccg aagaccgtct gaaagtgcac
     1501 ggcacaggcg gacgcttccc caaccgcact gccgatttgt ttttgggcaa cgcgggcacg
     1561 gcgttccgcc cgctgaccgc cgcgcttgcc gttttgggcg gcgattatcg cctgcacggc
     1621 gttgcgcgta tgcacgaacg ccccatcggc gatttggccg atgcactgcg gattgccggc
     1681 gccgatgtcg aatatctcgg caaggaacac tatccgccgc ttcatatcgg cgaacgccaa
     1741 gacaacggcg agcgcgtgat tccgattaaa ggcaatgtat ccagccagtt tctgaccgcc
     1801 cttttaatgg cgttgccgct gaccgggcag gcgtttgaaa tccgtatggt cggcgagtta
     1861 atttccaagc cctatatcga cattacttta aaactgatgg cgcaattcgg cgtacaggtt
     1921 atcaatgaag gctaccgcgt cttcaaaatt cccgccgatg cgcactacca cgcgcccgaa
     1981 cacttgcacg tcgaaggcga tgcctccagc gcgtcctact tcctcgcagc cggtttgatt
     2041 gccgccacgc ccgtccgcgt taccggtatc ggcgcaaaca gcatacaggg cgatgtcgcc
     2101 tttgcctgcg agctggaaaa aatcggtgcg gacgtggttt ggggcgaaaa cttcgtcgaa
     2161 gtctcgcgcc cgaaagaacg tgccgtccaa gcctttgatt tggatgcaaa ccatatcccc
     2221 gatgccgcca tgaccctcgc cgtcgtcgcg cttgctacag ggcaaacctg cacgcttcgc
     2281 aacatcggtt cgtggcgcgt caaagagacc gaccgcattg ccgcaatggc gaacgagttg
     2341 cgcaaactcg gtgcaaaagt cgccgaagaa gccgaagcaa ttcacatcac cccgcccgaa
     2401 acgccgacac ccgacgccgt catcgacact tacgacgacc accgcatggc aatgtgtttc
     2461 tcgctggttt cgctgttgga cgtacccgtc gtcatcaacg atccgaaatg cacccacaaa
     2521 accttcccga cttatttcga cgtgttctca tcgctgaccg aaacagcgga ataaggcggc
     2581 attttgccgc gattccggcg cggcgcggcg ggcggctcat tctgtaaaga aagtatgtgc
     2641 gccgaggtag tttttggcgt aaaacggtgt ggagagtttt tcggttttga tggttttgcc
     2701 gctgctgggg gcatggatga attcgccgtt gccgatatag agtccgacgt gtgagtagcg
     2761 gtgtgcgccg ccggtgttga agaatacgag gtcgccggcc ttaaggcggc tgtcggggat
     2821 tttgcggctt gccgccgcca tgtcgcgggc ggtgcgcggc agcttgacgt tgagggcgtt
     2881 tttgtaaacg aattgaatca tgccgctgca atcgaagccg gttgcggtgc tgctgccgcc
     2941 ccatttgtag ggcgtgccga tgagtccgag gctgtggagc atgagttcct gcgagccttg
     3001 tgtgcggtcg atgtggctga tgcggacggc ttggatttgc cggactgtct gtttgggttt
     3061 cggttggcgg tgtttgccgg aggtcgtgcc gcatgaggcg aggagcagtg cgctgagaca
     3121 gaggaaaagg gttttgtcgg ggggaaacat ggtttttcct ttgcgggttc ggatatgccg
     3181 tctgaaggtg tttcagacgg tatagtggat taa
//
LOCUS       DAWFIT010000139         3084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFIT010000139 DAWFIT010000000
VERSION     DAWFIT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.139"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            113..2878
                     /locus_tag="WGQ85_002073"
     CDS             113..2878
                     /locus_tag="WGQ85_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0751407.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYNPYKNYNNQELQKYIEDHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDNTLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
     gene            2908..>3084
                     /locus_tag="WGQ85_002074"
     CDS             2908..>3084
                     /locus_tag="WGQ85_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751408.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYE"
BASE COUNT          874 a          765 c          744 g          701 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcgcgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 ccccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 gacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agtggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atttggaacg gcgcaagcag cgatattttg tacaagaggg
      901 tgctttgaaa ttcaattccg acagcggaaa atgggagcgg gatttacaaa ggcaacagtg
      961 gaaatacaat ccgtataaaa attacaacaa ccaagaacta caaaaataca tcgaagacca
     1021 tgataaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggcaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gctcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 acttttaaag gattttgaaa cctacaacaa cgcgaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgcg aaaccgagtt gcaaaccacg ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtcctga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca agaaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggattgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggaa tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagta cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggcaga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagttc gttcgacccg aaagacaagg acgaagaagt
     2761 aacgtgtaat gatgataaca cgttatgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgataacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggttatttt
     3061 tggttgtttc gggtaattta tgag
//
LOCUS       DAWFIT010000140         3040 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFIT010000140 DAWFIT010000000
VERSION     DAWFIT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.140"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            44..2302
                     /locus_tag="WGQ85_002075"
     CDS             44..2302
                     /locus_tag="WGQ85_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0751409.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFSLLKGGPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2315..2980
                     /gene="pgmB"
                     /locus_tag="WGQ85_002076"
     CDS             2315..2980
                     /gene="pgmB"
                     /locus_tag="WGQ85_002076"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0751410.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
BASE COUNT          766 a          934 c          789 g          551 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttca taaatgtaca caagaatcat
       61 ggaaatcagc ccttggacgc tgcgttcggc aaaactggaa aaagaacaca aacggctgca
      121 agagagcctg accagcttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
      181 ctattccgcc gacagccact taggcaccta catcgccggc gtgtggttcc ccgacaaaac
      241 ccgcgtcggc tggtggaaaa acggctatcc caaatatttc ggcaaagcca tcaacgcgtt
      301 caatttcagc aaagtcaaaa tctttgtcga cgggcaggaa gtggacttgg cgaaaaacga
      361 cgttgccggc ttctccgtcg aactcgatat gcggcacggc gtgttgcgcc gctcgtttac
      421 cgtattcggt gtgcgtttca atgtgtgcaa attcctgtcc gtcgcgcaaa aagagctggc
      481 ggtcatccgc tgggaagccg tatccgttga cggtaaaacc caccaagtcc gcatcgattc
      541 catcatcgat gccgacgtga aaaacgaaga ctccaactac gaagaaaaat tctggcaggt
      601 attggacaaa ggcgtttcag acagtctctc ctacattgcc gcccaaaccg ttgccaaccc
      661 tttcggcgtg gaacaattta tcgtcaacgc cgagcaaact ttcgccggca gcttcaaagc
      721 cctcggcggc agccaaaccg actggcaagt ctccaattct ttcgaggtcg aagtcggcgg
      781 cacgcccaaa acctttgaaa aacgggttat tgttaccacc agccgcgatt atcagggctt
      841 ggaagcagtg aaagccgcag gccgcgcctt gtcggaaaaa gtcgcaggcg ttgcgtttga
      901 aaccttgctg gacgcgcaca aagcaggctg gctgcaccgt tgggaaatcg ccgacgtggt
      961 catcgaaggc agcgacgaag cgcagcaagg catccgtttc aatctgttcc aactgttctc
     1021 cacctactac ggcgaagatg cgcgcctgaa catcggcccc aaaggcttta ccggcgaaaa
     1081 atacggcggc gcgacctatt gggataccga agcctatgcc gtaccgctct acctcgcact
     1141 ggcggaaccc gaagttaccc gcaacctgct gcaataccgc cgcaaccaac tgccgcaggc
     1201 gcagcacaac gcgcgcgaac agggcttggc gggcgcactc tatccgatgg taacgtttac
     1261 gggtatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gcaacggcgc
     1321 gattccttac gccatctaca actacaccaa ctacaccggc gacgagggct atcttgccaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttctgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcacgg cgtaaccggc ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaca ccaacaccct cgccgcctgg gtattggact acacccgcga
     1561 agccctggcg aaatacccgc gcccggattt gaacgtgcgt gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gcatgacgaa gaactcggcg tattcgtcca
     1681 acacgacggc ttcctcgaca aagacatccg tcccgtgtcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaagcaggc
     1801 ggacgtgttg caaggcatct acttcttcgg cgaccgtttc aatatcgacg aaaaacgccg
     1861 caacttcgat ttctacgaac cgatgaccgt gcatgaaagc tcgctgtcgc cctgtatcca
     1921 ctctattctc gccgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggaca actacaacaa cgacacagaa gacggcctgc atatcacctc
     2041 catgaccggt tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cttggggcgg
     2101 caaactcagc ttcgcaccgt tcctgccggg cgcgtggaca ggctatgcct tccacatcaa
     2161 ctaccgaggc cgtctgatta aagtcgccgt cggcaaagaa aacgttgtct tcagcctgct
     2221 caaaggcggg ccgctcgatt tgcaggtgta cggcaaagac atcacgctca acggcagcca
     2281 caccgccgcg ttggaaaaat aaggagggcg caaaatgacg tttaccgcag tcctcttcga
     2341 tctcgacggc gtgattaccg ataccgccga ataccactac cgcgcgtgga aaaagctcgc
     2401 cgaagaactg ggcatcggca tcgaccgcaa gtttaacgaa cagctcaaag gcgtgtcgcg
     2461 cgacgattcg ctcaaacgca tcctcgcgca cggcggcaaa accgtcggcg aagccgagtt
     2521 cgccgaactg acccgccgaa aaaacgacaa ctacgtcgag atgattcagg cagtcaaacc
     2581 cgaagacgtg taccccggca ttttgccgct gctggagaca ttaaaagcaa acggcaaaaa
     2641 aatcgccctc gcgtccgcca gtaaaaacgg cccgttcctg ctcgaacgca tggggctgac
     2701 ccactttttc gacactgtcg ccgaccctgc cgccgtcgcg cattccaaac ccgcccccga
     2761 catcttcctc gccgccgccg agggcgtggg cgcggacatc cgccactgca tcggcattga
     2821 agacgccgcc gccggcgttg ccgccatcaa agccgccggc gcactgccca tcggcgtagg
     2881 caaagccgaa gacttgggca gcgacatcgc gctggtgtcc gagaccgccg ggctgaccta
     2941 cgcctacctg caaaacgtgt gggcgcagtc gggcaggtaa agcgcgtcag ataaagtgtt
     3001 aaggaagtaa aaggccgtct gaacagtgtt tcagacggcc
//
LOCUS       DAWFIT010000141         2991 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFIT010000141 DAWFIT010000000
VERSION     DAWFIT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.141"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            360..692
                     /locus_tag="WGQ85_002077"
                     /pseudo
     CDS             360..692
                     /locus_tag="WGQ85_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(909..1703)
                     /locus_tag="WGQ85_002078"
     CDS             complement(909..1703)
                     /locus_tag="WGQ85_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0751411.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1747..1851
                     /locus_tag="WGQ85_002079"
                     /pseudo
     CDS             1747..1851
                     /locus_tag="WGQ85_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1912..2637
                     /locus_tag="WGQ85_002080"
     CDS             1912..2637
                     /locus_tag="WGQ85_002080"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0751412.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          795 a          900 c          686 g          610 t
ORIGIN      
        1 attaaggggg tatttgggta gaattaggag gtatttgggg cgaaaacagc cgaaaacctg
       61 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcgg gctatgccgg
      121 ctgctgccgc ccattgcacc ttcgccgaat cctgccgccc accgccgaag ccctcatgcg
      181 ttcgcggtac agcgcgtatg tgctactata gtggattaac aaaaaccagt acagcgttgc
      241 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccgagt gaatcggttc
      301 cgtactattt gtactgtctt cggcttcgtc gccttgtcct gatttttgtt aatccactat
      361 acatcatcgc tactaccgtt ccggcacaac aaacattcct cgatgccgcc gaactgatgc
      421 agtggagcag agaaaccgaa tggctgggct tgaatgtcat cgcacaccgc aacttcggca
      481 aacagcacgc ccaagtcgaa tttgaagcct acttccgaga cggacaacac cgatccgcgc
      541 atcacgaact gtccggcttc atcaacatcg gcggacaatg gtattttatc gatcctaccg
      601 ttccgcatcc tgcgatgaaa caaccctgca tttgcggatc gggcaaaaaa ttcaaagcct
      661 gctgcggcaa atatctgaaa cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat
      721 tttcaacacg caaaaaaacc attcatacca agggtaagta tgaatggtca atacattgcg
      781 ggaaaacgtc ttacttgctg cactgccgaa aagggagaaa cggcagcggt aatcagcgga
      841 aaggattgta cccgaattaa tgttaagaaa cgttaatcgt gaaaatatat taacaaacct
      901 gttgaaacct attggttttc ccgtatccac tcgacccagc gttcaaacag cttcggttcg
      961 agcgcggcga cgaccgagcg tttgaacacg tgttcaccac tccaaaaccc gtcgccttcc
     1021 aaagtcgtca gcctgccgcc cgcctcctcg aaaatcaacg cgccggcggc ataatcccac
     1081 agcttctgcc cgccgtggac ataaacatca taacgcccgc acgccagata acaccagtcc
     1141 aacgtactgc tgcccatact ccgtatcgtt ccaaaaggcg cgagcgtact catgcggctg
     1201 gacagtttgc ccgaacgcaa atatttgatt tccacgcccg caatcgcctc attgagtttt
     1261 ttatccacga gacgcaaagg caggcgggtt ccattaagaa acgcaccctg cccccgttcg
     1321 gcataaaaac attcgccgct gaccgggttg tagattacgc ccaattccgc acgcccgttg
     1381 cggacaaacg ccaccgacac cgcaaaatgc ggcagcccgt tgacaaaatt gttcgtcccg
     1441 tctatcggat cgacaatcca cagccccttt tcccccgaat attgttccca caaagccgac
     1501 tgttcctgcc gcgacatttc ctcgcccaac atcgggcaat cgatcagaag cggcaacgcg
     1561 gcggcaaatg ccgtctgcgc ggcaatgtcc gcctcgctca acatcgaacc atcttccttg
     1621 cggcaagacg gcgtattcaa aaaacgcggc ataatttcgg tttgcgcgat atggcgcacg
     1681 actttctgca aacggtgtaa cacttcctac tgtcctcata ttttgaactt gcggcgcgcg
     1741 aacgtatata gtggattaaa tttaaatcag gacaaggcga cgaagccgca gacagtacaa
     1801 atagtacggc aaggcgaggc aacgctgtac tggtttaaat ttaatccact ataatgtccg
     1861 tttccatcac gccgctgcga cagattataa ccgtccgaac cgccaaaaac tatgccccga
     1921 ttccacctgc ccgaaaacct ttccgtcgga caaaccgtcg ccttgcccga caacatcgtc
     1981 cgccacctca acgtcctgcg cgtccgcccc aacgaaaaca tcaccctctt cgacggcaaa
     2041 ggcaaggcac acgccgcacg gctgtctttt ttggaaaaac gccgcgccga agccgaaatc
     2101 ctgcacgaag acacaaccga caacgaatcc ccgctcaaca ttacactgat acaatccatc
     2161 tcctccggcg atcgcatgga tttcaccctg caaaaaagcg tcgaactcgg cgtaaccgcc
     2221 atacagcccg tcatcagcga acgctgcatc gtccgcctcg acggggaacg cgccgccaaa
     2281 cgcctcgcac gctggcagga aatcgtcatc tccgcgtgcg aacaaagcgg cagaaacacc
     2341 gttccccccg tactgcccat catcggctat cgtgaagcac tcgacaaaat gccgtctgaa
     2401 agcaccaagc tgattatgag catcaaccgc gcccgcaaac tcggcgacat ccgccaccca
     2461 tccggcgcaa tcgtctttat ggtcgggccc gaaggcggct ggacagaaca ggaagaacaa
     2521 caggcatttg aagccggctt tcaggcggtt acactcggca aacggatttt acgcacagaa
     2581 accgccccac tcgccgccct cgccgcgatg cagacgcttt ggggcgattt cgcataaaca
     2641 gaaatgccgt ctgaaacccg ttcagacggc attttgcagc cgattaagat agtaggttca
     2701 aataagattt cccgtgtcgt cattcccgcg aaagcgggaa tctagaaacg aaaaactaca
     2761 gggatttatc cgaaacaaca accctctccg ccgtcattcc cgcgcaggcg ggaatctaga
     2821 aacgaaaaac tacagggatt tatccgaaac aacaaaccct ctccgccgtc attcccgcgc
     2881 aggcgggaat ctagaaattt aacgttgcgg tgatttatcg gaaatgactg aaactcaacg
     2941 gactggattc ccgcctgcgc gggaatgaca aagtggaagt tgcccgaaac t
//
LOCUS       DAWFIT010000142         2953 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFIT010000142 DAWFIT010000000
VERSION     DAWFIT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.142"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(139..1449)
                     /locus_tag="WGQ85_002081"
     CDS             complement(139..1449)
                     /locus_tag="WGQ85_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0751413.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1761..2183
                     /locus_tag="WGQ85_002082"
     CDS             1761..2183
                     /locus_tag="WGQ85_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751414.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2269..2766
                     /locus_tag="WGQ85_002083"
     CDS             2269..2766
                     /locus_tag="WGQ85_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0751415.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2871..2953
                     /locus_tag="WGQ85_002084"
                     /pseudo
     CDS             2871..2953
                     /locus_tag="WGQ85_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          808 a          714 c          716 g          715 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataccac cttgtcttta ttggctttat cgataaacag
       61 gataagacct gaaaaaagcc gatacgcctt tttggtgtac cggctttgcc atactgttct
      121 gcttcagacg gcatcgcttc attttgcctt taatacttct tcgtccagcg atttcaacca
      181 ttccagcttt tcgccgattt tgatttccaa cccgcgcggg acgggttggt agaagtccgg
      241 ttcgtccaaa ccgtcgggca tatagctttc gccggcggcg taggcgttcg gttcgtcgtg
      301 ggcgtagcgg tattcgcgtc cgtagcccaa ttccttcatc agcttggtcg gggcgttgcg
      361 caggtggacg ggcacttcgt cgctggcgtt ttctttgacg aagtggcgca tttggttgta
      421 tgccttgtag cccgcgttgg atttggcggc ggcagcaagg tacagcacgg cttgagccaa
      481 agccagttcg ccttcgggcg agcctaagcg ttcgaaggtg gcggcggcat cgttggcgat
      541 ttggaaggcg cgcgggtcgg caagcccgat gtcttcccaa gcgatacgca cgatgcggcg
      601 ggcgaggtag cgcgggtcgg tgccgccgtc gagcatacgg cagaaccaat acagcgcggc
      661 gttcggatgc gaaccgcgca cggatttgtg cagggcggag atttggttgt agaagctctc
      721 gccgcctttg tcgaaacggc ggatttgcgc cccgagactg tcggcgagaa attcggcggt
      781 taagtttttc agacgacgtg tatcggcggc gcgtaaaagt tgttccaaca aattcaacaa
      841 tctgcgcgca tcaccgtcgg cggtattgac gaacagctcc tgcgcgtcgg cttcaatcgt
      901 gaaatcccgg tattcgggca aagccaaaac tttggcaacc agctttttga ggccgtctga
      961 agacaagggc tgcaaaacat acacctgcgc gcggctcaac agcgcgggat tgacttcaaa
     1021 cgacggattt tccgtcgtcg caccaataaa ggttagcaaa ccgctttcga catgcggcaa
     1081 aaacgcgtcc tgctgcgcct tgttgaagcg gtggacttca tcgacaaaca aaatcgtcgc
     1141 gcgtccctgc tgcaaagcga tttcggcttt atcgattgcc tcgcggatgt ccttcacgcc
     1201 ggaaaacacg gcggaaacag gcaaaaactg ggcattgaaa ctctgcgcca aaatccgcgc
     1261 caacgtcgtc ttgcccacgc ccggcggccc ccacagcaac atagaatgcg gcttgccgcc
     1321 ttctaccgcc acgcgcaaag gtttaccttc gccgatgagg tgttcctgcc ccaccacgtc
     1381 gtcaagcgta tgcggacgca atcgttcggc aagcggcgcg tcgggttctc gggcaaacaa
     1441 atcggtcata acggctccgt caacaggttt tcaaacaata tgattatacg gcagggaacg
     1501 gcggcgtgcc gcatacggat tccgcccctc cgtttgcctt aagccgatat taggcgcata
     1561 ctggaaaaga cgagagactt cacacaatat atccggcacg gagaccgatt ccgcatcggc
     1621 atgacaatac ccaaatcagc gtttcaatta aacattaagg agactaaaat agaaaatttg
     1681 ctttatctac cattgctttg ttgattcaat cggcattatg ttttgaggcg gaagcccatg
     1741 aatatactaa tattcaagag atggaatggg tgtctttatt ttctgatccg caaagagacg
     1801 atgatagtct tataaccctt aaagatgaaa aaatcactgt aaaaaactat attgtgcctt
     1861 ggtggaaaaa aggtgaaaac tttagaaaat tagaacttat taccactggt aaaaatgata
     1921 atggaaattt aacggtaaat ggaatttacg gcatccaatc ttttgcagac tctcaattgg
     1981 gatatacgcc tgaattattg gtaaaaagca ataatgatat tctatttaaa aataatttcc
     2041 aatctgttga cgtgaagccc cattccggaa atatccattt ggatgccaaa ggaaaaattc
     2101 aatttaattt gaatgttccc gatgttgatg gggtacaaga tgtagccagt ggaaaaaaat
     2161 accgacaaac aatagtgcct tagtgcggta tgtcaacact atcagccccc cgatgaaaat
     2221 gacaaaggca gcatatcctt tcattcaaaa acaggcaatt tgttcaatat gaacgttaaa
     2281 gttcataaag acacacacca tttcggtatt cacaacctca acaataccga tgttcatctg
     2341 actgccgaag acagcgataa tgtgattgag attaaagggg aactcgccga ccttatgccg
     2401 acaggaaatg ttgagagtac aagtttttta aggggcattg aaacggcttt taaatccaat
     2461 accgtattaa cggggaataa caataaaata tccatcgcca ctgagggcaa gggcttggga
     2521 ttgggcgtaa atggtctcca gtccaactcc ccctattccg gacacacgac gggcattttc
     2581 atcaataatc aatcaagcga ccttacccaa ccttcccttg atggcatcag caccatcaaa
     2641 ctcaaagccg tcaagggaaa taatgaagtt gatttaaatg taaaaaacca cgcctccgta
     2701 aaaggcatta tcgcgtcgca ttctgcaaaa gcaacgcttg aagccgaagg ggacaatatt
     2761 gtctaggtta aaaatccgga caccaatacg gctttaatcg atgctttgaa aaaacaatac
     2821 cccaatgctt cgaaagaact atacagggtg ggggttcaat ccacgactgg aacagtacgc
     2881 atttcgccaa acggcaaaat cgcattaacg gaattgaaaa cttttggaat tgtacaaaac
     2941 ggcatttgcg tca
//
LOCUS       DAWFIT010000143         2866 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFIT010000143 DAWFIT010000000
VERSION     DAWFIT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.143"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..284
                     /locus_tag="WGQ85_002085"
     CDS             <1..284
                     /locus_tag="WGQ85_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751416.1"
                     /translation="ENRQHLKEHQLLDGIMRKACRNRPLSETQTKRNRYLSKTRYVVE
                     QSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            388..945
                     /locus_tag="WGQ85_002086"
     CDS             388..945
                     /locus_tag="WGQ85_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0751417.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGLCGELGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(1021..1989)
                     /gene="ispH"
                     /locus_tag="WGQ85_002087"
                     /gene_synonym="lytB"
     CDS             complement(1021..1989)
                     /gene="ispH"
                     /locus_tag="WGQ85_002087"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0751418.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(2018..2515)
                     /locus_tag="WGQ85_002088"
     CDS             complement(2018..2515)
                     /locus_tag="WGQ85_002088"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0751419.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          675 a          841 c          717 g          633 t
ORIGIN      
        1 aggaaaaccg gcagcatctg aaagaacatc agttactgga cggcattatg cgcaaagcct
       61 gccgcaaccg tccgctgtcg gaaacgcaaa ccaaacgcaa ccggtatttg tcgaagactc
      121 gttatgtggt tgaacagagc ttcggtacgc tgcaccgtaa attccgctat gcgcgggcag
      181 cctatttcgg gctgctcaaa gtgagtgcgc aaagccatct gaaagcgatg tgtttgaacc
      241 tgttgaaagc cgccaacagg ctaagtgcgc ccgctgccgc ctaaaaggca gcccggatgc
      301 ctgattatcg ggtatccggg gaggattaag gggggatttg ggtagaatta ggaggtattt
      361 ggggcgaaaa tagacgaaaa cctgtgtttg ggtttcggct gtcgggaggg aaaggaattt
      421 tgcaaaggtc tcagcctgcc cgaaatcatc cgcgacctgc tcgaaatgcc gtctgaaacc
      481 gccattgccg acatcacacg cacttattcc gcacattacc tcaatcccaa caaccgcaat
      541 atgacgctct ttcccgatgc cctgccctgt ctggacaagc tcaaagcaca aggatactgg
      601 cttgccgtcg ccacgggcaa agggcgggcg ggcttggaca acgccatcag ccaaaccgcc
      661 accggcggct attggctcgc caccgcctgc gcgggggaat atccctccaa accctcgccc
      721 gaaatggtat tcggactctg cggcgaactg ggactcgatc cgaaagaagc attggtcgtc
      781 ggcgatacgg cgcacgacct gcatatggcg gcaaacgcag gcgcggcggc agtcggcgtg
      841 gccaccggcg cacattcgcg cgaacagctc cttagcgcac cgcatctcgc cgtattggac
      901 gacttgtccg aactgcccgg ttttcttgca caacattacg cctgattggt ttccgcatca
      961 gacactcgaa acaaatgccg tctgaagcct gttcagacgg catttgtgtt gcccaaacat
     1021 tcaacgtttg cacaaatcgg gtttggtttc gccctcgcgg cgcagctctt tgggcaggac
     1081 gaacacaatg ctttcttccg caccctcgcc ttcgcgtacg gtttcgtgcc cccatccgcg
     1141 tatggttgcc agtacttccc gcaccaacac ttcgggcgcg gacgcgcctg ccgttacgcc
     1201 gactttgttt ttgccctcaa accatgcgcg ttgcaggtag cctgcattat ccaccatata
     1261 cgcatcgatt ccgcgcgatg ccgccacttc gcgcaagcgg ttgctgttgg acgaattggg
     1321 cgaaccgacc acaatcacga tgtcgcactg ttctgccaac tctttgacgg cggtttgccg
     1381 gttggtcgtc gcatagcaga tatcttcctt gtgcggattg cggatattgg ggaaacgcgc
     1441 gttcagcgcg gcgatgatgt ctttggtttc atcgaccgag agcgtggttt ggctgacata
     1501 ggcgagtttg tcggggtttc tgacttcgag ttttgccaca tctccgaccg tttcgaccaa
     1561 aagcattttg cccggcgcaa gctgccccat cgtgccttcg acctcgacgt gccccttatg
     1621 cccgatcatg atgatttcac agtcttgggc atccagtcgg gcgacttcct tatgcacttt
     1681 cgtcaccagc gggcaagtcg catcaaacac gcggaaaccg cgctccgccg cttcttgctg
     1741 caccgccttc gatacgccgt gtgccgaata aaccagtgtc gcgcccggcg gcacttccgc
     1801 caagtcttca ataaacaccg cacctttttc acgcaggttg tccacgacga atttgttgtg
     1861 aacgacttcg tggcgcacat aaatcggcgc gccgaactct tccaaagcac gttcgacaat
     1921 actgattgcc cgatccacac cagcgcagaa gccgcgcgga ttggcaagga tgatggtttt
     1981 ctcgttcata agcccggtat ttcgttttca gacggcatca atatttttct tcttgggttt
     2041 tacggtggac gatgttgtcc aacaccgcca acaccgcacc gacgcagata aagctgtcgg
     2101 caatattaaa ggcgggataa aaccaatttt gccaataaaa caataagaaa tcgacgacat
     2161 gaccgtgtat caggcgatcg atgacattgc ctaacgcacc gccgataatc atcgccgcac
     2221 ccgttttgcc gagggttgca aactcatcgc gcaagatggc gcgtaccaaa tacgcgctca
     2281 ccgccaccgc cagcaccaaa aaaaagtatt tttgccagcc gccctgatcg gcaaggaagc
     2341 tgaacgccgc acccgggttg tacaccaagg tcaaatcgaa aaaggaagga atgacattga
     2401 cgcgttcccg atactgaaac gacgacagca ccgcccactt cgaccactgg tccagcacga
     2461 tggcggcaag tgccaatacc caatagcgcg ttttacttga aacagatgaa gacatatttt
     2521 tcaacagccg gtaaaagagt accattttac ccgaaaaccc cctttcctgt acccgaaacg
     2581 gcaaatgccg taatcttaaa acccgtcatt cccgacaaca ccgtaatctc gaaacccgtc
     2641 attcccgcgc aggcgggaat ccagacctgt ccgcacagaa acttatcgga taaaaacagt
     2701 tgcccaaacc ccgcgttcta tagtggatta aattcaaacc agtacggcat tgcctcgcct
     2761 tagctcaaag agaacgattc tctaaggtgc tgaagcaccg agtgaatcgg ttccgtacta
     2821 tttgtactgt ctgcggcttc gttgccttgt cctgatttaa atttaa
//
LOCUS       DAWFIT010000144         2477 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFIT010000144 DAWFIT010000000
VERSION     DAWFIT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.144"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            633..2411
                     /locus_tag="WGQ85_002089"
     CDS             633..2411
                     /locus_tag="WGQ85_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0751420.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     AGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          769 a          609 c          610 g          489 t
ORIGIN      
        1 aatccataga aaaatcacag gaatctatca gaaaaaacag aaacccccac cgcgtcattc
       61 ccgcaaaagc gggaatccaa aaacgcaacg caacaggaat ctatcggaaa aaacagaaac
      121 cccaccgccg tcattcccgc aaaagcggga atccagaccc gtcggcacgg aatcttaccg
      181 gataaaacag tttccttaga ttccacgtcc tagattcccg ccttcgcggg aatgacgaga
      241 ttttagatta tgggaattta tcaggaatga ttgaatccat agaaaaacca caggaatcta
      301 tcagaaaaaa cagaaacccc caccgccgtc attcccgcgc aggcgggaat ccagcaacac
      361 aacgcggcag gactttatcg gaaaaaacag aaaccccacc gaccgtcatt cccgcaaaag
      421 cgggaatcca gacccgtcgg cacggaatct taccggataa aacagtttcc ttagattcca
      481 cgtcccagat tcccgccttc gcgggaatga cgagatttta agttggggga atttatcaga
      541 aaacccccaa cccccaaaaa ccgggcggat gccgcaccat ccgcccccaa accccgattt
      601 aaccattcaa acaaaccaaa agaaaaaaca aaatgaacaa aatataccgc atcatttgga
      661 atagtgccct caatgcctgg gtcgccgtat ccgagctcac acgcaaccac accaaacgcg
      721 cctccgcaac cgtgaagacc gccgtattgg cgacactgtt gtttgcaacg gttcaggcga
      781 atgctaccga tgaagatgaa gaagaagagt tagaatccgt acaacgctct gtcgtaggga
      841 gcattcaagc cagtatggaa ggcagcggcg aattggaaac gatatcatta tcaatgacta
      901 acgacagcaa ggaatttgta gacccataca tagtagttac cctcaaagcc ggcgacaacc
      961 tgaaaatcaa acaaaacacc aatgaaaaca ccaatgccag tagcttcacc tactcgctga
     1021 aaaaagacct cacaggcctg atcaatgttg aaactgaaaa attatcgttt ggcgcaaacg
     1081 gcaagaaagt caacatcata agcgacacca aaggcttgaa tttcgcgaaa gaaacggctg
     1141 ggacgaacgg cgacaccacg gttcatctga acggtatcgg ttcgactttg accgatacgc
     1201 ttgcgggttc ttctgcttct cacgttgatg cgggtaacca aagtacacat tacactcgtg
     1261 cagcaagtat taaggatgtg ttgaatgcgg gttggaatat taagggtgtt aaaactggct
     1321 caacaactgg tcaatcagaa aatgtcgatt tcgtccgcac ttacgacaca gtcgagttct
     1381 tgagcgcaga tacgaaaaca acgactgtta atgtggaaag caaagacaac ggcaagagaa
     1441 ccgaagttaa aatcggtgcg aagacttctg ttattaaaga aaaagacggt aagttggtta
     1501 ctggtaaagg caaaggcgag aatggttctt ctacagacga aggcgaaggc ttagtgactg
     1561 caaaagaagt gattgatgca gtaaacaagg ctggttggag aatgaaaaca acaaccgcta
     1621 atggtcaaac aggtcaagct gacaagtttg aaaccgttac atcaggcaca aatgtaacct
     1681 ttgctagtgg taaaggtaca actgcgactg taagtaaaga tgatcaaggc aacatcactg
     1741 ttatgtatga tgtaaatgtc ggcgatgccc taaacgtcaa tcagctgcaa aacagcggtt
     1801 ggaatttgga ttccaaagcg gttgcaggtt cttcgggcaa agtcatcagc ggcaatgttt
     1861 cgccgagcaa gggaaagatg gatgaaaccg tcaacattaa tgccggcaac aacatcgaga
     1921 ttagccgcaa cggtaaaaat atcgacatcg ccacttcgat ggcgccgcag ttttccagcg
     1981 tttcgctcgg cgcgggggca gatgcgccca ctttaagcgt ggatgacgag ggcgcgttga
     2041 atgtcggcag caaggatgcc aacaaacccg tccgcattac caatgtcgcc ccgggcgtta
     2101 aagaggggga tgttacaaac gtcgcacaac ttaaaggcgt ggcgcaaaac ttgaacaacc
     2161 gcatcgacaa tgtggacggc aacgcgcgtg cgggcatcgc ccaagcgatt gcaaccgcag
     2221 gtctggttca ggcgtatctg cccggcaaga gtatgatggc gatcggcggc ggcacttatc
     2281 gcggcgaagc cggttacgcc atcggctact ccagtatttc cgacggcgga aattggatta
     2341 tcaaaggcac ggcttccggc aattcgcgcg gccatttcgg tgcttccgca tctgtcggtt
     2401 atcagtggta agggcttttt cgcctgtctg ctgttgggac aggcggaagg tttgaaggga
     2461 agggtggcga tttgccg
//
LOCUS       DAWFIT010000145         2468 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFIT010000145 DAWFIT010000000
VERSION     DAWFIT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.145"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(13..819)
                     /locus_tag="WGQ85_002090"
     CDS             complement(13..819)
                     /locus_tag="WGQ85_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0751421.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="WGQ85_002091"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="WGQ85_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0751422.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          684 a          622 c          639 g          523 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taagtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattct
//
LOCUS       DAWFIT010000146         2458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFIT010000146 DAWFIT010000000
VERSION     DAWFIT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.146"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            35..1390
                     /locus_tag="WGQ85_002092"
     CDS             35..1390
                     /locus_tag="WGQ85_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0751423.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WGQ85_002093"
     CDS             1394..2404
                     /locus_tag="WGQ85_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0751424.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          660 c          740 g          605 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWFIT010000147         2427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFIT010000147 DAWFIT010000000
VERSION     DAWFIT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.147"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            128..2341
                     /locus_tag="WGQ85_002094"
     CDS             128..2341
                     /locus_tag="WGQ85_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0751425.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPIPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          638 a          781 c          575 g          433 t
ORIGIN      
        1 aggaaaaatc agaaaagttt tgcattttga aaatgagatt gagcataaaa ttttagtaac
       61 ctatgttatt gcaaaggtct caagctgtta caataaactc gtttttatcg gaacggaaga
      121 ccccatcatg accgccatca gcccgattca agacacgcaa agcgcgactc tgcaagaatt
      181 gcgcgaatgg ttcgacagct actgcgccgc gctgccgaac aacgataaaa aacttgtctt
      241 agccgcccgt tcgctggcgg aagcacatta ccccgccgat gccgccacgc cgtatggcga
      301 gccgctgccc gaccacttcc tcggcgcggc gcaaatggtt catgaactcg acctgctccc
      361 cgatgccgtc gccgccaccc tgcttgccga catcggacgc tacgtccccg actggaacct
      421 attggtttcc gaacgctgca acagtaccgt cgccgagctg gtcaaaggtg tggacgaagt
      481 gcagaaactc acccacttcg cccgggtgga cagcctcgcc acaccggaag aacgcgccca
      541 gcaggcagaa actatgcgga aaatgctgct ggcgatggtt accgacatcc gcgtcgtgtt
      601 aatcaaactg gcgatgcgta cgcgcaccct gcaattttta agcaacgccc ccgacagccc
      661 cgaaaaacgc gccgtcgcca aagaaaccct cgacatcttc gccccgctcg ccaaccgttt
      721 gggcgtgtgg cagctcaaat ggcagctcga agatttgggc ttccgccatc aagaacccga
      781 aaaataccgc gaaattgccc tgcttttgga cgaaaaacgc accgaacgcc tcgaatacat
      841 cgaaaacttc ctcaatatcc tgcgtacgga actcaaaaaa tacaatatcc actttgaagt
      901 cgccggccgt ccgaaacaca tctactccat ttacaaaaaa atggtgaaga aaaaactcag
      961 cttcgacggc ctctttgaca tccgcgccgt gcggattctg attgataccg tccccgagtg
     1021 ttacaccacg ctgggcatcg tccacagcct ctggcagccc attcccggcg agttcgacga
     1081 ctacatcgcc aaccccaaag gcaacggcta taaaagtttg cacaccgtca tcgtcggccc
     1141 ggaagacaaa ggcgtggaag tgcaaatccg caccttcgat atgcaccaat tcaacgaatt
     1201 cggtgtcgcc gcccactggc gttacaaaga gggcggcaaa ggcgattccg cctacgaaca
     1261 gaaaatcgcc tggttgcgcc aactcttgga ctggcgcgaa aacatggcgg aaagtggcaa
     1321 ggaagacctc gccgccgcct tcaaaaccga gcttttcaac gacacgattt atgtcctgac
     1381 cccgcacggc aaagtcctct ccctgcccac gggcgcgacc cccatcgact tcgcctacgc
     1441 cctgcacagc agcatcggcg accgttgccg cggtgcgaaa gtcgaagggc agattgtgcc
     1501 gctgtccacc ccgctcgaaa acggacagcg cgtcgaaatc attaccgcca aagaagggca
     1561 tccttccgtc aactggctct acgaaggctg ggtcaaatcc aacaaggcaa tcggcaaaat
     1621 ccgcgcctac atccgccagc aaaacgccga caccgtgcgc gaagaaggcc gcgtccaact
     1681 cgacaaacag cttgccaaac tcacgcccaa acccaacctg caagagcttg ccgaaaatct
     1741 cggctacaaa aagccagaag acctctacac cgccgtcgga caaggcgaaa tttccaaccg
     1801 cgccatccaa aaagcctgcg gcacgctgaa cgaaccgccg cccatacccg tcagcgaaac
     1861 caccatcgtc aaacagtcca aaatcaaaaa aggcggcaaa aacggcgtgc tcatcgacgg
     1921 cgaagacggt ctgatgacca cgcttgccaa atgctgcaaa cccgcgccgc ccgacgacat
     1981 tgtcggcttc gttacccgcg atcgcggcat ttcggtacac cgcaaaacct gcccctcttt
     2041 ccgacacctc gccgaacaag cacctgaaaa agtgctggac gcaagctggg cggcgttgca
     2101 ggaaggacaa gtgttcgccg tcgatatcga aatccgcgcc caagaccgct ccgggctttt
     2161 gcgcgacgta tccgacgcac tcgcccgcca caaactcaac gttaccgccg tgcaaaccca
     2221 gtcccgcgac ttggaagcca gcatgaggtt cacgctcgaa gtcaaacaag ttaccgacct
     2281 cccgcgcgtc ctcgccagcc tcggcgacgt caaaggcgta ttgagcgtta cccggcttta
     2341 aatacaaaaa tgccgtctga aagccgtata acgcttcaga cggcattttg attaccaggg
     2401 tttgctattt tttgttgcat agtcaat
//
LOCUS       DAWFIT010000148         2314 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFIT010000148 DAWFIT010000000
VERSION     DAWFIT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.148"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGQ85_002095"
     CDS             complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGQ85_002095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0751426.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1633..2199
                     /locus_tag="WGQ85_002096"
     CDS             1633..2199
                     /locus_tag="WGQ85_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0751427.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          529 a          564 c          665 g          556 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct taaatttaat ccactatatt ttcctgtttc
       61 aggctcggcg ttctttgcac atcagtttcg catccagcca gccatcgccg ccggagatga
      121 tgttgttggc aacgaagttg tccaagtcgg gcagcaggga aacggcgcgg ccgctgttga
      181 tgatgacgac caccgccatc ggtttgtcgc ccagccaata acctgcaagg gcgcggacat
      241 tgttgagcgt gccggttttt aagcgcaaca gcccgccgct ttgtttgaag cggttgcgta
      301 aagtcccgtc tgtgccggcg atgggcagcg tatcgatgaa atcttgtgca aacgggctga
      361 aataagccgt ttccaacatt tgcgccatca ttctcgccgt taccctttct tttctggaca
      421 gacctgaacc gttttccaaa accaaatccg caacatcgat gcccgacacg gcaagttcac
      481 gccggacggc agacgctgcc tgttcggaaa cggcgggcag tttgccgtcg ccgccgagtt
      541 tgaggaagac ggaacgcgca attagattgt ccgaacgctt gttcatgtcc gtcaaaattt
      601 ccttcatcgg ctttgagtgt gcaacggcaa gcgtctgcgc gccttccggc gtgtcggata
      661 tgccgatgcc gtctgaaatc cgtccgccgc cgagcagcca gtggttggta aaactttgcc
      721 ggatcagttc gtcaagcgcg aacatccgga caccgacagg cttgcccaaa cagctctcgg
      781 gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga tgcacgcatc agttttttga
      841 tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt gttttgggcg aaaatatgcg
      901 gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc attgcgttcg gcgcgcacca
      961 taaccatacc ggcagacagc atagttggat tggggggcgt cataaacggc gaaccgctgt
     1021 cggcttcgaa atcgtcgggg ctgccgactt cgccccacag gctgtggtcg agcatcaggt
     1081 gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg ttggacggca agcaggtttt
     1141 cctgattgaa aacggggtcg ccgctgcccg cccaatacag gtttccgtca agcgtgccgt
     1201 cgtttaccgt accgttgctt ttaaactcgg tcgcccagcg gtaattgctg ccgaaggttt
     1261 tgaaggcggc aaacgcggta acgagtttca ttgtggaggc ggggttgacg gggacatccg
     1321 agcggtggtc aatgatgact tttccgctgt caagctcttg gacatatacg gcgatttcgt
     1381 tttgcggaat gcgacctgta tcgagcgcgt gtgcggcgag ggaggcgaga agcagcagca
     1441 gggaggccgc tgttttgggg aaattcatag gtaaatctgt tacataaaaa aagcaattat
     1501 aaggcaaagc cggataagtg ggaacgctgt ggcggcgggc cggcttgttt gcaggggaaa
     1561 tcacatatat aataatcgct atcattatga aatgattgag acgcacaaac ttaatattca
     1621 ggaggaatga ttatggctaa gaaaatcagt attttggtgg gcagcctgcg ccgtgcttct
     1681 tttgcgcgca aagtggcatt gaatgcggcg gagatgttcc ccgaaggctg gcaggcggaa
     1741 atcgtcgaaa tcggacatct gccgctttac aatttcgatt atgacgaccc tgcggtggaa
     1801 gatgtgccgc tgcccgaaag ctacacggtt ttccgcgaaa cgattaaggc ttcggacggc
     1861 attttgttcg ttacgtccga aaataaccgc accattcccg cctgtttgaa aaatgcggtg
     1921 gacatcggct cgaaaccgaa tgccgacgtg gcgtggaaaa acaaaccggc cggcatcatc
     1981 agccattccg tcggcaagat gggcggttac agttcgcaaa aaaacctgcg ccttgcgctg
     2041 tcttatttcg atatgcccgt aaccggacag ccggaagtgt ttttgggcaa ttcgccgaca
     2101 ctgtttgatg aaaacggcaa attgatcgac tcggcaaggg attttgttca gtcatatatt
     2161 aatcagtttg tcggtttgat tgaaagaaat gccaaataaa caaacaagtc gaaaggccgc
     2221 aaaaccggtt cgggttttgc ggctttttta tatgggctgg gcagcgtcaa ggtttgccgt
     2281 ttgccggata atgcgcggca aatgaccggc gtgt
//
LOCUS       DAWFIT010000149         2295 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFIT010000149 DAWFIT010000000
VERSION     DAWFIT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.149"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(14..601)
                     /locus_tag="WGQ85_002097"
     CDS             complement(14..601)
                     /locus_tag="WGQ85_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0751428.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            764..2170
                     /gene="dnaB"
                     /locus_tag="WGQ85_002098"
     CDS             764..2170
                     /gene="dnaB"
                     /locus_tag="WGQ85_002098"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0751429.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          563 a          589 c          619 g          524 t
ORIGIN      
        1 ggattaacaa aaatcaggac aaggcggcga agcgtttggc gacttcgtcc cagttgacga
       61 tttcccaaaa acctttcagg tagttggggc ggctgttgcg gtagtcgatg taataggcgt
      121 gttcccacac gtcgcaggtc agcagcggcg tgttttcagt ggtcagcggc gtagcggcgt
      181 tggaagtgga aaccaaatcc aatccgccgg caggggtttt caccagccat gcccaaccgg
      241 agccgaaagt accggcagcg caggcattga acgcttcttg gaatttctcg aagctgcccc
      301 atttcgcgtc gatggcggcg gccagttcgc cggaaggttt gccttgacct ttgggcgtga
      361 aacccagcca gtagaaggtg tggttccaag tttgcgccgc gttgttgaac acgccgcccg
      421 aagatttttt cacaatctct tccaaaggca ggttttcaaa ttcggtgcct ttgatttgat
      481 tattcaggtt ggtgatgtag gtttgatggt gtttgccgta gtggaactcc aaagtctctt
      541 tgctcagatg cggggacaat gcgtccagtt cataaggcag ttgcggcagc ttatgttcca
      601 ttttgtgctc ctaatattgt tttaaatgtt gtattttggc agtgttgctg caaatagctc
      661 agcagcgcac atattctacc tgttttgagg gatggaaacc aattaaacct gctttacgct
      721 ataatagaag attgcaattt cggcacgaca gataggatgt accatgaacg attacacagc
      781 tatgccgtct gaagaccgtg aggtcggcgc attgtcgctg cctccacatt cgatggaggc
      841 ggaacaatcc gttttgggcg gtttgatgct ggaaaatccg gcttgggaca ggattgccga
      901 tgtggtttcg ggagaggatt tctaccgcca cgaacaccgc ctgattttcc gatccattgc
      961 aaaattaatc aatgagagcc gtcccgccga tgtgattacg gttcaggaag atttgcagcg
     1021 gaacgaagaa ttggaagcgg caggcggatt tgaatatctg attacgctgg cgcaaaacac
     1081 cccgtctgcc gccaacatcc gccgctatgc cgaaatcgtg cgcgagcgtt ccatcatgcg
     1141 ccagctcgcc gaagtgggga cggaaatcgc acgcagcgca tacaatccgc aaggcaggga
     1201 cgcggggcag cttttggacg aggcggaaaa caaagtattc caaatcgccg aaagcacggc
     1261 caaatccaag cagggctttt tggagatgcc cgatttgctg aaagaagtcg tacagcgcat
     1321 cgatatgctc tactcgcgcg acaatcccga tgaagttacc ggcgtgccga cagggttcat
     1381 cgacctcgac aaaaaaacct cgggtctgca acccggcgac ctgattatcg ttgccggtcg
     1441 tccgtctatg ggtaagaccg ccttttccat caatatcgcc gaacacgttg ccgtagaagg
     1501 caagctgccg gttgccgtct tctcaatgga gatgggcggt gcgcaactgg tcatgcgtat
     1561 gctcggctcg gtcggacggt tggatcaaag cgtcctgaaa acaggcaggc tcgaagacga
     1621 acactggggt cgcctgaacg aagcagtcgt caaactctcc gacgcgcccg tgtacatcga
     1681 cgagaccccg ggtttgactg cgctcgaact gcgcgcccgc gcccgccgtc tcgcccgtca
     1741 atttaacaat aagctgggat taatcgtcat cgactacctg caactgatgg cgggatccgg
     1801 ccgttccgac aaccgagctt cagagctggg cgaaatttca cgttcgctca aagcgttggc
     1861 gaaagaattg caagtcccca tcatcgccct gtcgcaactg agccgcacgg tcgaatcgcg
     1921 taccgacaaa cgcccgatga tgtccgacct tcgcgagtcc ggcgcaatcg agcaggatgc
     1981 cgacctgatt atgtttatgt accgcgacga atactacaac caagactcgc ccatgaaagg
     2041 ccttgccgaa tgtatcatcg gcaaacaccg caacggtccc gtcggtaaaa tcttcctcac
     2101 atggacgggg caattcacca aattcgacaa tgctgcctat attcccgagg aggcaaagat
     2161 agaagattaa atggctatat aaaaatttat taggcgaaat caggcaaaat cgtttaaaat
     2221 catgctgaga gattgcccta aaaaataaaa cgcggtcttg aggcattttt gcattcagcc
     2281 cgcatataat tgaaa
//
LOCUS       DAWFIT010000150         2259 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFIT010000150 DAWFIT010000000
VERSION     DAWFIT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.150"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..979
                     /gene="rfbB"
                     /locus_tag="WGQ85_002099"
     CDS             <1..979
                     /gene="rfbB"
                     /locus_tag="WGQ85_002099"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0751430.1"
                     /translation="NLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAELDRVFAQHR
                     PDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYWQQMPSEKREAFRFHHI
                     STDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNN
                     YGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGET
                     YNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVPDRPGHDVRYAVDAA
                     KIGRELGWKPQETFESGIRKTMQWYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            1176..2042
                     /gene="rfbA"
                     /locus_tag="WGQ85_002100"
     CDS             1176..2042
                     /gene="rfbA"
                     /locus_tag="WGQ85_002100"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0751431.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2081..>2259
                     /locus_tag="WGQ85_002101"
     CDS             2081..>2259
                     /locus_tag="WGQ85_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEZ0751432.1"
                     /translation="MKDKKMNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQV
                     CERTFVQENHSKSGKG"
BASE COUNT          582 a          640 c          550 g          487 t
ORIGIN      
        1 caacctcgac aaactgacct acgcgggcaa tctcgaatcg ctgaccgaca ttgccgataa
       61 tccccgctat gctttcgagc aagtggacat ttgcgaccgc gccgaactcg accgcgtatt
      121 cgcacaacac cggcccgatg ccgtgatgca cttggcggcg gaaagccatg tcgaccgctc
      181 tatcggttcg gcaggcgagt ttatccaaac caatatcgtc ggcacattca atctgcttga
      241 agcagcccgc gcctactggc aacaaatgcc gtctgaaaag cgcgaagcct tccgtttcca
      301 ccatatttcc accgatgaag tctatggcga tttaggcggc acggacgatt tgtttaccga
      361 aaccgcgccc tacgcgccgt ccagccccta ctctgcctct aaagcgtcca gcgaccacct
      421 cgtccgcgcg tggttgcgta cttacggctt gccgaccatt gtaaccaact gctccaacaa
      481 ctacggtcct taccattttc cggaaaaact cattcctttg atgattctga acgcgcttga
      541 cggcaaaccg ctgcccgttt acggcgacgg tatgcaaatc cgcgactggc tgtttgtcga
      601 agaccacgcg cgcgcactgt atcaggttgt taccgaaggt gttgtcggcg aaacctacaa
      661 tatcggcggc cacaatgaaa aagccaatat tgaagtcgtc aaaaccatct gcgccctgct
      721 ggaagaactc gctcccgaaa aaccggccgg tgtggcacgg tatgaagatt tgattacctt
      781 tgtcccagac cgccccggcc acgatgtgcg ctacgccgtc gacgcagcca aaatcggtag
      841 ggagctgggc tggaaacctc aagaaacatt cgaatccggc atccgcaaaa caatgcaatg
      901 gtatttggac aacaaaacct ggtggcaaaa tgtattgaac ggtagctatc gtctagaacg
      961 tttaggtacg ggcaaatagt tttcagacgg catcccgacg caatgccgtc tgaaaaccta
     1021 tcgcaaagga agaaagaaaa gatgtttcaa cacacaggac ggcacataaa gcgtcgccct
     1081 atgtgccgtc ctgatttgga aggggttacc ccctcccaaa taaagtctga ttctaccgcc
     1141 ctaaagggcg gggtttcaac cgaaaaggaa atacgatgaa aggcatcatc cttgccggcg
     1201 gcagcggcac gcgcctctac cccatcacgc gcggcgtatc caaacagctc ctgcccgtgt
     1261 acgacaaacc gatgatttat taccccttgt cggttttgat gctggcagga atccgcgata
     1321 ttttggtgat taccgcgcct gaagacaacg cctctttcaa acgcctgctt ggcgacggca
     1381 gcgatttcgg catttccatc agttatgccg tgcaacccag tccggacggc ttggcacagg
     1441 catttatcat cggcgaagaa tttatcggca acgacaatgt ttgcttggtt ttgggcgaca
     1501 atatttttta cggtcagtcg tttacgcaaa cattgaaaca ggcggcagcg caaacgcacg
     1561 gcgcaaccgt gtttgcttat caggtcaaaa accccgaacg tttcggcgtg gttgaattta
     1621 acgaaaactt ccgcgccgtt tccatcgaag aaaaaccgca acggcccaaa tccgattggg
     1681 cggtaaccgg cttgtatttc tacgacaacc gcgccgtcga gttcgccaaa cagctcaaac
     1741 cgtccgcacg cggcgaattg gaaatttccg acctcaacca aatgtatttg gaagacggtt
     1801 cattgtccgt ccaaatatta ggacgcggtt tcgcgtggct ggacaccggt acgcacgaaa
     1861 gcctgcacga ggcggcttcg ttcgtccaaa ccgtgcaaaa catccaaaac ctgcacatcg
     1921 cctgcctgga agaaatcgct tggcgcaacg gttggctttc cgatgaaaaa ctggaagaat
     1981 tggcgcgccc gatggcgaaa aaccaatacg gccaatattt gctgcgcctg ttgaaaaaat
     2041 aatgtttgag gccgtctgaa acttttcaga cggcctttag atgaaagata aaaagatgaa
     2101 catcattgat accgccattc ctgacgtaaa actgcttgag ccccaagtct tcggcgacgc
     2161 gcgcggcttt tttatggaaa ccttccgcga cgagtggttt aaaacccaag tctgcgaacg
     2221 caccttcgtg caggaaaacc actccaaatc cggcaaagg
//
LOCUS       DAWFIT010000151         2169 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFIT010000151 DAWFIT010000000
VERSION     DAWFIT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.151"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            33..466
                     /locus_tag="WGQ85_002102"
                     /pseudo
     CDS             33..466
                     /locus_tag="WGQ85_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            562..1185
                     /gene="rsmG"
                     /locus_tag="WGQ85_002103"
     CDS             562..1185
                     /gene="rsmG"
                     /locus_tag="WGQ85_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0751433.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1284..2057
                     /locus_tag="WGQ85_002104"
     CDS             1284..2057
                     /locus_tag="WGQ85_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0751434.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          433 a          493 c          741 g          502 t
ORIGIN      
        1 aatgcggtct gcttcggaag agggggacgg cgatgtttgt gaacgagaaa gatccttatg
       61 cggctctgtt tgcgggactg gtgtttttga cgctgccgtt tgcgttggcg gtgcatgatg
      121 cctttgcgct tgcgttcgga cgggcggggc tgctggtgtc ggtgtcggac ggcggattcg
      181 gctggcgtgg cggttgggac ggcactgttt ggtttgtgtt cggtgtgttt gcgtttttga
      241 atgtggttgt gtcggcgggc ctgacgaaac tggcgtacaa aaagatgatg cggcggcatt
      301 cgcgtggatt ttcgagctgc tgtttggcag tgccgccttg ggcgggctgc gtggggaggc
      361 ggtgttggaa tatgcgtttg ccgtgtggct ggtgtcgatg ctgacactgc ccaaacgctt
      421 gacgcgcgcg ccggtgcagc cggtggtgtt tcacaggaaa aaataggacg gaacggggga
      481 tgccgtctga aacccgacac gcggtttcag acggcatgtt tttccgttaa cattacgcct
      541 gaacatggac aggataggga tatggaacgc aaagaacgcc tgcgtgcagg catcgccgca
      601 ttgggtttgg atatttccgc agcggcgcag gacaggcttt tggcctatgt ggatttgttg
      661 aaaaagtgga acaaaaccta caatctgacc gccctgcgcg acgaggaaaa aatggttgtc
      721 catcatcttt tggacagcct gacgctgctg ccccatatcg agggtgtgca aacgatgctg
      781 gatgtcggtt cgggcggcgg tcagcccggc attccggcgg cggtgtgccg tccggatgtg
      841 caaataaccc ttttggatgc gaatacgaag aaaacggctt ttttacagca ggcggttatc
      901 gagttggggt tggacaatgt gcgcgtggta tccggacgcg tggaggcggt ttcggacgtg
      961 cgtgccgacg tggttaccag ccgtgcgttt gcagaactgg cggactttgt atcgtggacg
     1021 gcgcatctgt tgaaagacgg cggctactgg gcggcgatga agggcgtata tccgcagggg
     1081 gaaatcggcc gcctgccgca ggatgtgtgc gttgaaaaag tccaaaggct cgacgtgcca
     1141 ggcttggatg cggaacgcca tatcgtcatc ctgagcaagc gttgagcgcg cttcagacgg
     1201 catgaatacc ttttttgtgc ggataaaggt aaaattccgc actgtttttc ttttttcaac
     1261 atcagacggg acacgggcgg gacatgagtg cgaacatcct tgccatcgcc aatcagaagg
     1321 gcggtgtggg caaaacgacg acgacggtaa atttggcggc ttcgctggca tcgcgcggca
     1381 aacgcgtgct ggtggtcgat ttggatccgc agggcaatgc gacgacgggc agcggcatcg
     1441 acaaggcgag tttgcagtcc ggcgtttatc aggtcttatt gggcgatgcg gacgtgaaat
     1501 cggcggcggt acgcagcaaa gagggcggat acggcgtgtt gggtgcgaac cgcgcgctgg
     1561 ccggcgcgga aatcgagctg gtgcaggaaa tcgcccggga agtgcgtttg aaaaacgcgc
     1621 tcaaggcagt ggcggaagat tacgacttta tcctgatcga ctgcccgcct tcgctgacgc
     1681 tgttgacgct taacggcttg gtggcggcag gcggcgtgat tgtgccgatg ttgtgcgaat
     1741 attacgcgct ggaagggatt tccgatttga ttgcgaccgt gcgcaaaatc cgtcaggcgg
     1801 tcaatcccga tttggatatc acgggcatcg tgcgtacgat gtacgacagc cgcagcaggc
     1861 tagttgccga agtcagcgaa cagttgcgca gccatttcgg ggatttgctg tttgaaaccg
     1921 tcatcccgcg caatatccgc cttgcggaag cgccgagcca cggtatgccg gtgatggctt
     1981 atgatgcgca ggcaaagggt gccaaggcgt atcttgcctt ggcggacgag ctgatggcga
     2041 gggtgtcggg gaaataggtc aatccaaatc gggctgcccg tgcctttatg ctgtttggcc
     2101 gggtgcgtta atatggcgga ttaaaataaa aatacttata tcgtcattta tagtggatta
     2161 acaaaaacc
//
LOCUS       DAWFIT010000152         2139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFIT010000152 DAWFIT010000000
VERSION     DAWFIT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.152"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..291
                     /locus_tag="WGQ85_002105"
     CDS             <1..291
                     /locus_tag="WGQ85_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0751435.1"
                     /translation="EAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDN
                     YSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            308..>2139
                     /gene="frpC"
                     /locus_tag="WGQ85_002106"
     CDS             308..>2139
                     /gene="frpC"
                     /locus_tag="WGQ85_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ0751436.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQ
                     VVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDG
                     HQCLAPWVKETKKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATG
                     WVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINA
                     ADAAFQSLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGS
                     YTKTNGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIG"
BASE COUNT          703 a          399 c          495 g          542 t
ORIGIN      
        1 gaggcttatg aagataaaag acatattcct gaaaatccat attttcatga attttactat
       61 attaaaaaag gagaaaatcc ggcgattatt actcattgga ataatcgagt aaaccaggct
      121 gaagaagata attatagcac tagcgtaggt tcctgtatta acggtttcac ggtacagtat
      181 tacccgttta ttcgggaaaa gcagcagctc acacagcagg agttggtagg ttatcaccaa
      241 caagtagagc aattggtaca gagttttgta aacaattcaa gtaaaaaata atttaaagga
      301 tcttattatg aatgagggtg aagttgtttt aacaccagaa caaatccaaa ccttgcgtgg
      361 ttatgcttcc cgtggcgata cctatggcgg ttggcgttat ttggctaatt tgggtgaccg
      421 ttatgcggat gatgctgctg caattgtcgg taaggatgca aacttaaatg gtttgaattt
      481 atggatgaaa aaaggtgtgg aaaacctatg ggatgatacg gtcggtaaaa agacccgttt
      541 agagaaattt gatcgggttg cactgcaaca tttcaggcaa tatgcgcgtc taattaatca
      601 aaataatggt agattaccca atactagtga aattgagaga agttactata aagccgttac
      661 cgataatggc gtttcttcca gtgcagctat tgatttagtt attaatcgtt cacttccgga
      721 tatggcggat ggttattggg cattaggttt ggggatagaa gccgaacgta tccacaatga
      781 gcaagcagta aataatccga acggtagcga aagggataat agaaagcagt taatatctgc
      841 tttagataaa ggatttgatg gatcttttaa agagaagcat tttacttttt tacaatctgt
      901 gatgatggat gtaacaaagt taggtgttga atatacaata gatggttggc aaaaaattgg
      961 aggttggggt aatgggataa tcaatgattt atataaaagt gttgtaaaaa gagagtggac
     1021 tggaatattt gagatcgtta ataataacat caagcaagga aatgaagctt ttaaaaatga
     1081 aatcaatagc ttggttcatg atatgaaagc tgctggcaag gaatttggag atgacttaaa
     1141 tacacagtgg aataatctca ctcaggctgc cgaaataatc tataatgaca tagtagacaa
     1201 tactagtcaa ggaatagaaa aaggtgtcaa agccattaaa gaattgtctg aaaaaatgaa
     1261 aaatgctgct tccgatttgg ctgacggttc agcagagaaa gctaaacaag tagtggaaga
     1321 tttggctcaa gccgccaaag aagcatacga aaatgccaaa tccacagccg agaaggctgc
     1381 tcaagcagct cgagaatttt ttaagggctt gcccagtttt aaagatctgg ccgaaaaatt
     1441 tagagatctg ttcccaaatc cggaaggctg gatcgatgat ggtcaccaat gtttagctcc
     1501 ttgggttaaa gaaactaaaa aacgcaatgg caaatatcat gtctacgacc cccttgccct
     1561 agacctagat ggcgacggta tagaaaccgt tgccaccaaa ggctttgcag gcagcttatt
     1621 tgatcacacc aacaacggta tccgcaccgc caccggttgg gtttctgccg atgacggttt
     1681 actcgtccgc gatttgaacg gcaacggcat catcgacaac ggtgcggaac tcttcggcga
     1741 caacaccaaa ctggcagacg gctcttttgc caaacacggc tacgcggctt tggccgaatt
     1801 ggattcaaac ggcgacaaca tcatcaacgc ggcagacgcc gcattccaat ccctgcgtgt
     1861 atggcaggat ctcaaccagg acggcatttc ccaaaccaac gaactccgca cccttgaaga
     1921 attgggtatt caatctttgg atctcgccta taaagatgta aataaaaatc tcggtaacgg
     1981 taacactttg gctcagcaag gcagctacac caaaacaaac ggtacaaccg caaaaatggg
     2041 ggatttactt ttagcagccg acaacctgca cagccgcttc aaagacaaag tggaactcac
     2101 tgccgaacag gcaaaagccg ccaatcttgc gggcattgg
//
LOCUS       DAWFIT010000153         2114 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFIT010000153 DAWFIT010000000
VERSION     DAWFIT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.153"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            413..1870
                     /locus_tag="WGQ85_002107"
     CDS             413..1870
                     /locus_tag="WGQ85_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0751437.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          485 a          619 c          487 g          523 t
ORIGIN      
        1 gacggtcttt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
       61 aaacagtacg gaaccaattc acttggtgct tcagcacctt agagaatcgt tctctttgag
      121 ctaaggcgag gcaacgccgt actggttttt gttaatccac tataaaaaac cgccctatcg
      181 gataagtcgc ttgactgaaa aggctttacc cgcgccgtat cggaacacgt cctctaaaat
      241 acaatccgtt gaattgaaac aaatataaaa acattcgacc gcaaaaacgg cagcgcatcg
      301 tttgacaaag attgaaaata tcggttaaaa accgattttc atacaaaaaa caccgctgcc
      361 gtccgcatcc gtttcagacg gtattgagag aaaatctttt aggagaacct ttatgtcccg
      421 gcatcccgcc cgcaccggag aaaaaacatt cttcggccac cccttccagc tttccaccct
      481 cttccatatc gaattgtggg aacgtttttc attttacgga atgcagggca tcctgctgat
      541 ttacctctac tacaccgccg acaaaggcgg cttgggcata gacaaaaccc tcgccggcgg
      601 cattgtcggc gcatacagcg gcagcgtgta cctgtccacc attttgggcg cgtggtttgc
      661 cgaccgcgta tggggtgcgg aaaagaccct cttcctctcg ggcatcgtcg tgatgctcgg
      721 acacatcgtc cttgccgccg ccccgggcct gtacggcctt ttaatcgggc tgatattcat
      781 cgcattgggc agcggcggtg tgaaatctac ggccagttct atggtgggcg cattatacga
      841 acaggacgaa atgcgcccgc tgcgcgatgc gggattttcc attttctaca tcgccatcaa
      901 tatcggcggc ttcctaggcc cgctgctgac cggcctgctg caggaaaaca tcggtttcca
      961 ttatggtttc ggcgcggcgg cggtcggtat ggcattcggc ttgtggcgtt attccttggg
     1021 acgtaaaaac ctgccccacc ccaccgtccc ccatccgctt tcaaaaggac agggcaaaac
     1081 tgcggccgcc gtcggcatcg ccctcatcgc cgcacttgca accgccatca aaaccgggct
     1141 tgtcaacctc gacaatttct ccggcatcct cctatctacc gtcatccttg ccgtcatcgt
     1201 ctattttacc cggctgctga ccaacccccg cgtcagttcc gacaacaagc ggcacatcat
     1261 cgcctacatc ccgcttttcc tgaccatctg tatgttttgg gcggtctggt ttcagattta
     1321 caccgtggca accgtctatt tcgacgaaac cgtcaaccgc accatcggtt cgtttaccgt
     1381 acccgtcgct tggaaagatt ctatgcaaag cctgtgggtc atcctgtttt ccggacttat
     1441 ggcggcaatg tggacaaaaa tggggcgcaa acagcccaaa accccgctga aattcgctat
     1501 ggcggtattt gttaccggcg cgtcgttttt gggattcgtc ccctttattt ccgccggcac
     1561 gccgatgcct attgcggttt tcgcactgat tgtccttgcc atcacgatag gcgaactgat
     1621 gatttccccg attgcgctgt ccatctccac caaaatcgca ccgcctttat tcaaaaccca
     1681 aatggtcgcc cttaatttcc ttgccttttc attaggcttc actttgggcg gcgtattgtt
     1741 tgaaaaaggc tatcaggcgg gcgacgaaat cggcttctat cggctgctgt tctacatcgg
     1801 cgcagccaca ggcttcctgc tgctcctgct cgtccccaaa ttgaacaaaa tgctcgaagg
     1861 cacagactaa gtcccgcccc gatgccgtct aaacccttca gacggcattt ttccgcataa
     1921 tgaaaccaaa ccgtttccac ccgacaggac aggctccccc ccaaccggaa ggcagcctgc
     1981 cgattgtcat ttaaatatct gactgattcc gtctgtttgc aagggaaata gccgtttatt
     2041 tccgcttggg cggaaaccgg ttctattgag taaaaggcat tttatccgac taaattagtg
     2101 ctgcatcaac ggaa
//
LOCUS       DAWFIT010000154         2099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFIT010000154 DAWFIT010000000
VERSION     DAWFIT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.154"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..1443
                     /locus_tag="WGQ85_002108"
     CDS             <1..1443
                     /locus_tag="WGQ85_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0751438.1"
                     /translation="INGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYITHKIA
                     HAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            1448..1831
                     /locus_tag="WGQ85_002109"
     CDS             1448..1831
                     /locus_tag="WGQ85_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751439.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
BASE COUNT          719 a          391 c          429 g          560 t
ORIGIN      
        1 atcaacggcg gcagcctcaa agacaacttg ggcgatgccg cactgggtgc gatagtcagt
       61 accgtacacg gagaagtagc gagcaaaatc aaatttaatc tcagcgaaga ctacattacc
      121 cacaagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggtaagtgt
      181 caggatggtg cgatcggtgc ggctgtgggc gagatagtcg gggaggcttt gacaaacggc
      241 aaaaatcctg ccactttgac agctaaagaa cgcgaacaga ttttggcata cagcaaactg
      301 gttgccggta cggtaagcgg tgtggtcggc ggcgatgtga atacagcggc gaatgcggct
      361 aaagtcgcga ttgaaaataa cctattatct caagaagagt atgctcttag agaaaaattg
      421 atcaaaaaag ccaaagggaa aggcctatta tctttagatt ggggcagcct gaccgaacaa
      481 gaggcaaggc agtttatcta tttgattgag aaagatcgat attctaatca attgcttgac
      541 cgatatcaaa aaaatccaag tagtttaaat aatcaagaaa aaaatattct tgcatatttt
      601 attaaccaaa cctctggagg taacacagct tgggcagctt cgatactgaa aacgccccag
      661 tcaatgggta atctcactat tccttccaaa gatattaata acaccttatc gaaagcctat
      721 caaacattga gtcgttatga ttcttttgat tacaaatcag ctgttgccgc acaacctgca
      781 ctttacttat taaacggacc gcttggcttc agtgtcaaag cagctactgt ggcagcagga
      841 ggatataaca ttggacaggg agcgaaagca atctctaatg gagaatatct gcatggtaca
      901 gttcaggttg ttaatggcac attgatggtt gcaggatctg tatctgcaca ggctgcaata
      961 tcggccaagc ctgcacctgt tacccgttat ctgagcaatg acagtgctcc tgctttaaga
     1021 caagctttaa ctgctgaaag ccagagaatc cgcatgaaac tgccggaaga gtatcgacaa
     1081 atagggaatc ttgcgatagc aaaaattgat gttaaaggat taccgcaaag gatggaagca
     1141 tttagttctt tccaaaaagg ggaacatgga tttatttcgt tacctgaaac aaaaattttt
     1201 aaacctatat ctgttgataa atatcataat attgcctctc ctcctagagg aacattaaga
     1261 aatatagatg gagaatataa attacttgaa actatagcac agcaactcgg aaataatcgt
     1321 aatgtatcag gtagaattga tctatttaca gaattaaagg cctgtcaatc ttgcagcaat
     1381 gttattttag agtttagaaa tcgctatcca aatattcaat taaatatttt tacaggaaaa
     1441 tagaatcatg gaaaaaataa aaaattatat agaattcaag aaattattta aaaatataac
     1501 ggaaaaattt gtcagagatt tagatataac attaattgaa gcagctgaat attctataat
     1561 tgaattcgaa atttatttat ctgaatatcc ggaagatagt acatttattt ttacagcatg
     1621 ggcaatctat ctgttagaaa ataactgtct caatgaaaga ggaaaagata attttttcat
     1681 cgcggaaatg aaaaaatgtt atcaacaaac taatttggat agaataaaat ctatctattc
     1741 ttcgagtgac tatcatattt ttgctaagga tgtaaacaag attcaacaaa catttaaatt
     1801 aaatcttgcc aatatcatgc agcaagagta gcaactgtat ttttaacccc gtcgggcaaa
     1861 attaccaaaa acggtaaatc actaaaaata cttttatacc gccgccgcag caagctgtag
     1921 cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctc cagcacgcac gcgttccatg
     1981 atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt tgttatgcaa
     2041 gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga cacccccta
//
LOCUS       DAWFIT010000155         2051 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFIT010000155 DAWFIT010000000
VERSION     DAWFIT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.155"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(264..695)
                     /locus_tag="WGQ85_002110"
     CDS             complement(264..695)
                     /locus_tag="WGQ85_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751440.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(711..>2051)
                     /locus_tag="WGQ85_002111"
     CDS             complement(711..>2051)
                     /locus_tag="WGQ85_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0751441.1"
                     /translation="VNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAH
                     AIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAG
                     TVSGVVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKY
                     QNVADKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFS
                     KSYTQADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFV
                     KQNTPITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVK
                     SETQTDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAA
                     SQGYSKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          544 a          409 c          377 g          721 t
ORIGIN      
        1 gaactgatat ccgtggtgct gagcaggatg cgactgttct cattggcata cggtgcgcta
       61 atgctcacac cggttttacc cgaaagttct gcagcaagcg tagggggtgt cttcagcacg
      121 cacgcgtctg ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg
      181 aaaaattgtg agcttttcag acggcattga gccgtaaatc atggaacgcg tgcgtgctga
      241 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagtttgt ttgagaatat
      301 gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa gaaatcagat
      361 attgcaatat taaagtaatg atatgctaaa ttaataggta taattaattc ttcaggattg
      421 aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc aaatccattt
      481 acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg agcacctatt
      541 actccaaaat gtccggtagt tgtaatattt ttaaaacact ctaaaagcgt atctttagtt
      601 tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt atttatagat
      661 tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa ttattctggg
      721 tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt gattccgtca
      781 aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt tctttcattt
      841 tgagcaattt tagaggcttt tgaatatcct tgtgaagcag cattttgagc catttgaagt
      901 attttatcat cagaaaattt tttaggatta taaacagttt ttatacttga aatttcctta
      961 aatccaccat caggtttacc tgtcctgtct agtgtaggaa tctcatattt aattcgggta
     1021 atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga atttagttct
     1081 gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact aaaaccatca
     1141 gcatttgcca gatgtctttt taggtttgta tcgaaactga tgccttccgg gtatttgaca
     1201 ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa tctaggatta
     1261 actcctgaaa taagtgtata accttggtcg gacatccatt cggataaagg ctttgtattg
     1321 cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa agccaaatct
     1381 gcttgggtgt aagatttgct gaataattta taccattcat catctttacc aattagacct
     1441 gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct gattgttcta
     1501 cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag tcttttatca
     1561 gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt attctgtttg
     1621 gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag ctgattattt
     1681 ttcaccgcta cctcagccgc attcgccgcc gcatttacat cgccgccgac cacaccgctt
     1741 accgtaccgg caaccagttt gctgtatgcc aaaatctgtt cgcgttcttt agctgtcaaa
     1801 gtgtcaggat ttttgccgtt tgtcaaagcc tccccgacta tctcgcccac agccgcacct
     1861 atcgcaccat cctgacactt gcccttattc gccgccgctg ccgcacagcc cgctatggca
     1921 tgggcaatct tgtggactat gtagtgctga tccaactgtt tgattttact ggctgcttct
     1981 ccatgcgcgg tattgaccaa agccgcaagg atattcgctt ccagattgtc tttcaggctg
     2041 ccgccgttga c
//
LOCUS       DAWFIT010000156         1944 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFIT010000156 DAWFIT010000000
VERSION     DAWFIT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.156"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            294..1481
                     /locus_tag="WGQ85_002112"
     CDS             294..1481
                     /locus_tag="WGQ85_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0751442.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGAGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVS
                     VAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFK
                     RHDDMSVSVRYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQRPTLVPAVVGK
                     PGSDVYYAGLNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDQAKGTDPLKNHQ
                     VHRLTGGYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGF
                     DLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLR
                     HKF"
BASE COUNT          498 a          444 c          504 g          498 t
ORIGIN      
        1 gccgcgtgtg ttttttatgg cgttttaaaa agccgggact gcatccgggc agcagcgcat
       61 cggcccgcac gaggtctgcg cttgaattgt gttgtagaaa cacaacggtt tttgaaaaaa
      121 taagctattg ttttatatca aaatataatc atttttaaaa taaaggttgc ggcatttatc
      181 agatatttgt tctgaaaaat ggttttttgc gggggggggg gtataattga agacgtatcg
      241 ggtgtttgcc cgatgttttt aggtttttat caaatttaca aaaggaagcc gatatgcgaa
      301 aaaaacttac cgccctcgta ttgtccgcac tgccgcttgc ggccgttgcc gatgtcagcc
      361 tgtacggcga aatcaaagcc ggcgtggaag gcaggaacta ccagctgcaa ttgactgaag
      421 cacaagccgc taacggtgga gcgggagcga gcggtcaggt aaaagttact aaagttacta
      481 aggccaaaag ccgcatcagg acgaaaatca gcgatttcgg ctcgtttatc ggctttaagg
      541 ggagcgagga tttgggcgaa gggctgaagg ctgtttggca gcttgagcaa gacgtatccg
      601 ttgccggcgg cggcgcgacc cagtggggca acagggaatc ctttatcggc ttggcaggcg
      661 aattcggtac gctgcgcgcc ggtcgcgttg cgaatcagtt tgacgatgcc agccaagcca
      721 ttgatccttg ggacagcaat aatgatgtgg cttcgcaatt gggtattttc aaacgccacg
      781 acgatatgtc ggtttccgta cgctacgatt cccccgaatt ttccggtttt agcggcagcg
      841 tccaattcgt tccggcccaa aacagcaagt ccgcctatac gccggctcat tttgttcaga
      901 ataagcaaaa tcagcggcct actctcgttc cggctgttgt cggcaagccg gggtcggatg
      961 tgtattatgc cggtctgaat tacaaaaatg gcggttttgc cgggaactat gcctttaaat
     1021 acgcgaaaca cgccaatgtg ggccgtgatg cttttgagtt gttcttgctc ggcagcggga
     1081 gtgatcaagc caaaggtacc gatcccttga aaaaccatca ggtacaccgc ctgacgggcg
     1141 gctatgagga aggcggcttg aatctcgcct tggcggccca gttggatttg tctgaaaatg
     1201 ccgacaaaac caaaaacagt acgaccgaaa ttgccgccac tgcttcctac cgcttcggta
     1261 atgcagttcc acgcatcagc tatgcccatg gtttcgactt gatcgaacgc ggtaaaaaag
     1321 gcgaaaatac cagctacgat caaatcatcg ccggcgttga ttatgatttt tccaaacgca
     1381 cttccgccat cgtgtctggc gcttggctga aacgcaatac cggcatcggc aactacactc
     1441 aaattaatgc cgcctccgtc ggtttgcgcc acaaattcta aatagcgggg cggtgaagcg
     1501 gatagctttg tttttgacgg cttgccttca ttctttgatt gcaatctgac tgccaatctg
     1561 cttcagcccc aaacaaaaat ccggatacgg aagaaaaacg gcaataaaga cagcaaatac
     1621 cgtctgaaag attttcagac ggtatttcgc atttttggct tggtttgcac atatagtggg
     1681 ggactgttag tttttgcaca aggaacaaat agagtaaaaa aacgctgaaa tctcggaaag
     1741 acgtggattt cggcgttttt ttgcatccgg aaaagttacg ccagcttttt cacgaaatcg
     1801 cgccggaatg cgcggttttc tgtttaaagc tgacgagatt agggaatttt taaaactgtt
     1861 ttaagaggtt tttaaaatgg atttaatcaa tactccggcc ataccactca acctgtgttg
     1921 ccgctttgac gaactgagca tccc
//
LOCUS       DAWFIT010000157         1836 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFIT010000157 DAWFIT010000000
VERSION     DAWFIT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.157"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..258
                     /locus_tag="WGQ85_002113"
     CDS             <1..258
                     /locus_tag="WGQ85_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751443.1"
                     /translation="RQLLDGIMRKAHRNRPLTEVQTKRNRYLSKTRYVVEQSFGTLHR
                     KFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            406..585
                     /locus_tag="WGQ85_002114"
                     /pseudo
     CDS             406..585
                     /locus_tag="WGQ85_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(582..1088)
                     /locus_tag="WGQ85_002115"
     CDS             complement(582..1088)
                     /locus_tag="WGQ85_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0751444.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(1091..1288)
                     /locus_tag="WGQ85_002116"
     CDS             complement(1091..1288)
                     /locus_tag="WGQ85_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751445.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(1285..1551)
                     /locus_tag="WGQ85_002117"
     CDS             complement(1285..1551)
                     /locus_tag="WGQ85_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751446.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1563..>1836)
                     /locus_tag="WGQ85_002118"
     CDS             complement(1563..>1836)
                     /locus_tag="WGQ85_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751447.1"
                     /translation="SRAREQEPFVIHVSDLLDKAGIDYAVRMDKDFQTTFHLEYPITN
                     YDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVPV"
BASE COUNT          446 a          499 c          433 g          458 t
ORIGIN      
        1 cgtcagttgt tagacggcat tatgcgcaaa gcccaccgca accgtccgct gacggaagtg
       61 caaaccaaac gcaaccggta tttatcgaag acccgttatg tggtcgaaca aagcttcggt
      121 acgctgcacc gtaaattccg ctatgcgcgg gcagcctatt tcgggctgct caaagtgagt
      181 gcgcaaagcc acctgaaggc gatgtgtttg aacctgttga aagccgccaa caggttaagt
      241 gcgcccgctg ccgcctaaaa ggcagcccgg atgcctgatt atcgggtatc cggggaggat
      301 taaggggata tttgggtaaa attaggaggt atttggggcg aaaacagccg aaaacctgtg
      361 tttgggtttc ggctgtcggg agggaaagga attttgcaaa ggtctcgggc tttaattgcg
      421 gcaaaaatag accaagcgga gatttctgat tcagaaaaaa gctggctgaa aaaagaatta
      481 gggaaaatca aggacactgc attaagtacg cttacggaaa atgcaattaa tgctatcctt
      541 gccgccaccc tcattactct tttaaaaaaa tttgtcggcc tctaaccaaa cttcttctcc
      601 acttccaccc caaacggctc caatacctcc acaaaggcag gcaaatgttt ggggtgtttt
      661 tcttgcacaa acgccatcaa gaactcaatc aattccaaag ccgtcgccag ttttgacgtt
      721 tccggcatca cacgcgcatt ggcggatacg gttttcgtaa acgcatcggc caggctggcc
      781 aacagcttgg cgcggtcgga cgggggcaaa tcttcggtac tcgaatcctg cagcatcgtc
      841 atcgtgctgt tgtactgcac cataaaaccg gccaacatcg cacggctcaa gtcctcaata
      901 ccgccgccgg ccaaagtgta ggcggcgcgc atcttatccc agtcgtcgcc tttttcctta
      961 tccgcacgtt tccacgcacg cgcagtggtc tgcgggattt cgcacatcaa ggccgccgtt
     1021 tccaaagttt gctcgccgct cacatagagc cgacgtaact tttcacggat ttcttgcggg
     1081 tgagccataa ttacagtccc attttcgctt taagcaattc ccagccgacc gtaatcacgc
     1141 cgccgcccag tgcgccgaat gtaatggccg tgcgtttcgt gtcttggcgg atttgcgcaa
     1201 tttccgcctg catttctttc tgatttttca gcgtttgatc agtcttgttt tcaatacgcg
     1261 ccaaggcttc caaaatcggg tcgctcatga tttgtctgct ttcctgtcca gtttttcgtt
     1321 tactttttcc aacttgtttt cgatgcgtcc caacgaagcc gcaatattgt cgcggtctgc
     1381 cttggcatct tgcttggtgt gataagagag cttgaccgcg tgcagctcct ctttcaggtc
     1441 gtcgatacgc ttgtccgcct ctttcagacg gcctgaaatg ccgttgaccc aaaaccaaaa
     1501 tgccgccgtc gcaatcggcc acagggttct gaaaccaaat tcaaagtcca tttaaaaccc
     1561 ctttaaaccg gcacatcgcc gaatacgata cggacggcgt aaccgtcagg gcgattgctg
     1621 gcaatctcca gtccatcacc atcattacaa acacagtaat acgcccccaa agtttgccaa
     1681 accgcacgtt taaaggtgtc atagttcgta attggatatt caaggtgaaa cgtcgtctga
     1741 aaatccttat ccatgcgtac cgcgtaatcg ataccggcct tatccaacaa atcggaaaca
     1801 tgaatgacaa acggctcttg ctcgcgtgcg cggctt
//
LOCUS       DAWFIT010000158         1769 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFIT010000158 DAWFIT010000000
VERSION     DAWFIT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.158"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(410..1399)
                     /gene="porB"
                     /locus_tag="WGQ85_002119"
     CDS             complement(410..1399)
                     /gene="porB"
                     /locus_tag="WGQ85_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0751448.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          457 a          391 c          453 g          468 t
ORIGIN      
        1 atgtaggttc gtaggaatga cgtgatgcag gtttccgtat ggatggattc gtcattcccg
       61 cgcaggcggg aatctaggtc tgtcggtgcg gaaacttatc ggataaaacg gtttcttgag
      121 attttacgtt ctggattccc actttcgtgg gaatgacggg attaaagttt caaaatttat
      181 tctaaatagc tgaaactcaa cgcactagat tcccgcctgc gcgggaatga cggcatattt
      241 tgacattgaa taaaaaagac taaaaacaga aaacagccaa acagaaaaca gcgaaacagg
      301 aaaaagccaa acagaaaaaa gccaaacaga aaaaagccaa acagaaaaaa gccaaacaga
      361 aaaaagcctg tctggcgaca ggctttttgt tgataccaat ctttgcagat tagaatttgt
      421 ggcgcagacc gacaccgccg gcagtcgcta cgaatttgtt ttcgcctttg ccttcttgca
      481 accaaccggc agaaaccaag gcagaagtgc gtttggagaa gtcgtattcc gcaccgacaa
      541 ccacttggtc gtaagtattg tcgcgttttg catcatcaac cgagcctttg aagccgtggg
      601 cgtaagaaac gcggggcgtt acgttgccga agcggtatgc caaggtagcg gcaacttcgg
      661 tttgagagtt gtgcgaattg tcttcaacca gtttcgcgtc ttgttgctgt acggctacgg
      721 aagcgtatag ggcatcattg tcgtaaccgc tgaccaaacg gtgaatctgg tatttctcaa
      781 tcttcacgtc atccacatcc tgatgtcttt tataggcacc gccatattgc acgaagaagc
      841 cgccgttttt gtagttgaag ccggcgtggt aagattcgct gttatgtctg cctgcattgt
      901 cgttaagcgc gtattgtacg ctgccgctga ggccggcaaa ttcgggagaa tcgtagcgta
      961 cggaaatgag gcgtgcctcg ggttcggcaa ttttgtttac acccaaatag tcgcttttgc
     1021 tatcccaagg attgatgtcg ccggtgtctt tcaggacgct gttcaaacgg ccgacgcgca
     1081 atttaccgaa gccgcctttc aagccgatga aggattggcg gttgccccaa ccggagtcag
     1141 taccggcgat agatgctttt tgctcaacct gccaaatggc tttcaggccg ttaccgaggt
     1201 cttcttggcc tttgaagccg attttcgaac ccaaatcaac gatgccggta ccggtttcaa
     1261 cgctataaac ttgaccgtcc tggtaatcta gggagcggga agtttctacg ccggctttga
     1321 tggtgccgta cagggtaacg tcagccattg ctgcaacagg aagggctgcc aaagtcaggg
     1381 caatcaggga ttttttcatt gctgtattcc ttttttggtt aagaaattta agcaggccgg
     1441 gctttccaag ccgcttagct ttgcatttac cgccgagggg ctcggcgatt acatgtctga
     1501 aactatagat gttcctgtct gaaaaaacaa acttctgcct gtttttgtgt ggaattcgat
     1561 gtgcattttg aaggacggat agagatatta tgggtatttt ttgttttata acatatggtt
     1621 atttaaattt tttaagattt gcatttttac aacacttact cgggagggta ttggagggca
     1681 ttgcaaaccg ggggttataa agacggcaaa aaacgcacac gcggtttctt tcggaacggg
     1741 cggatgcaaa acccgcccct gcggcagga
//
LOCUS       DAWFIT010000159         1731 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFIT010000159 DAWFIT010000000
VERSION     DAWFIT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.159"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            357..1661
                     /locus_tag="WGQ85_002120"
     CDS             357..1661
                     /locus_tag="WGQ85_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0751449.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          352 a          434 c          513 g          432 t
ORIGIN      
        1 caattaaaaa tttttaatat attaatcaat aaattaattt tataaaataa aaatattgtc
       61 aacaatattt tgccttatcg cccaaacctc tgtatatttt cctacagtaa attgttgaca
      121 atccatacgc ccacatatgc gccgcctaag gataaatcct cccgccggac aacgggtgca
      181 agggattgga tgcgatattt ccatattcaa acaagggatt tgtttcacgc acagggcggc
      241 acatcggcaa aatccccgcg ccgcgccggt ccggcagggc ttgcgtccct cccggacaag
      301 ccccgacccc gcctttccga aagacgggct caaccattaa ggaaacttta atcaaaatga
      361 aaaaacacat atgggcggca tctttgctgc cggcatccct atcggcagaa cctttaaact
      421 ggtggaagcc ttattccgcc gtcaattcgg gcgataccgc ctgggtgatg actgcggctg
      481 ccttggtact gttgatgacg cttcccgggc tggctttatt ctacggcggt atggtgcgga
      541 aaaaaaacct gctctcgacg atgatgcaca gcttttccat cgcgacattg gtgggcatcc
      601 tttgggtcgc cgtcggctat tctttagcgt tcacgccggg aaatgccttt atcggcggtt
      661 tggggcgcgt atttttaagc gggatgcaga tagacgctgc cgcacagatg ctgaccgtgt
      721 cgcccaatgc gccgactgtt cccgaaccgg tatttatgtt ttttcagatg acgtttgcca
      781 ttatttcgac cgccattatt accggcgcgt ttgccgaacg gatgaaatat tcggcaatga
      841 tgctgttttc gggcatatgg tttttattgg tttatgtgcc gggcgcgcat tgggtgtggg
      901 gcggcggctt tatgagcaag ggcggcgtat tggattatgc cggcggtacg gtggtgcaca
      961 tcaatgccgg tatcgcggga ctcgtcgccg ccttggtttt gggcaggcgc ataggctacg
     1021 ggcgcgaggc gatgcctccg cacaatatgg cgatgacact gatcggcgcg gcaatgttgt
     1081 ggttcggctg gttcggcttt aacgccggat cggcgcttgc ggcagacgcg gcggcgggta
     1141 tggcgatggc ggtaacgcag gtgtcggccg tattcggcgc ggcaggctgg cttgcctgcg
     1201 aaaaaatagc gggacacaaa ccttccgctt tggggctggc ttccggcgcg gtttccggtc
     1261 tggtcggcat cacccctgcc gccggcttta ccggcccgtc gggcgcggcc gccatcggta
     1321 tattgactgc cgccgcgtgc tttgtgtccg tcaccgtcgt caaacacaaa ttgcgttacg
     1381 atgattcttt ggacgctttc ggcatacacg gattcggcgg gctggtgggc ggaatattga
     1441 ccggcatctt tttcgacaac cgcattttcg gcggggatgc ggcagtttgg cagcagttgt
     1501 ggatacaggt aaaagacggg ttcgttatgg cggcatacag cgggctgatg agttgggcga
     1561 ttttgaaggt cgtggggaaa atctgcggcg gcctgcgcgt cggcaaggat gtcgaacgcg
     1621 aaggtttgga tctgaatatc cacggcgaac gcgtggaata agggcggcta tgccgtctga
     1681 agcctgaaaa tcgggtttca gacggcattt ttcacgtttg ccgccgatgg a
//
LOCUS       DAWFIT010000160         1647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFIT010000160 DAWFIT010000000
VERSION     DAWFIT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.160"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..876
                     /locus_tag="WGQ85_002121"
     CDS             <1..876
                     /locus_tag="WGQ85_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0751450.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRD
                     TQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIF
                     ALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQN
                     PPSFIKGTKLELKFQ"
     gene            895..1269
                     /locus_tag="WGQ85_002122"
     CDS             895..1269
                     /locus_tag="WGQ85_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0751451.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          516 a          340 c          369 g          422 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 attatccaag gtattatgcc tgcacatgca gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatgggg tttatgctgc aggttggaca gctccgctga tcggtacagc gggtaaatta
      241 gctatcagca cctgcatggc taatccttct ggttgtactg tcatggtcac tcaggctgcc
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagcgcct
      361 gtgggggcgt tgtcgaaagc gaaggcggct aagcaagctg ctcctaaaga aacaataaac
      421 aatttggcaa atttagccaa agcagaacag cagattttat tccgtattgc ccaacgcgat
      481 acgcaactgg atgcatggaa gacgggattt aacaatagag taaggaaagg agcaggcttg
      541 cttgatgcaa gtaatattcc gataaccatt aacggaaaaa ccatcaaacc tgtacaagcc
      601 ataagcttaa agggagcacc cgtttacagc ggcgtaagcg aacaggagat ttttgcgctt
      661 tatcggcaga tgactggcca gaatccgaat tttagagttt tgcctgacgg aagattagca
      721 aatggcatta tcagtactgg agaatgggca ggaacaaaaa ttgcattaag aaatttttca
      781 aaaacagaga attcaactca agcacgatgg acattagatt tgcagaatcc tccatcattt
      841 attaaaggta ctaaattgga gcttaaattc caataattta caaaggattt taccgtggat
      901 gagaaacaaa aaattaagat tcttgatttt caaatcgatt tatcctcaat ttttaactct
      961 tataaaaatc aaatgggtat taatattcaa gataaaaact taaaaaacaa tttctgttct
     1021 tttatggaag aactgttaaa tgacggttca atccgtttac atgattatac cgacggtatc
     1081 ggaattcctc taactggaac ttcaaaagaa caagtgcaga aattgaaaga catatggcct
     1141 actttggaag atgcccaagc aatatggcct gaagaccctt ggtattactt agaatggctt
     1201 tggtgggata ttgcgtgtcc aatagatttg gccgatttgc cgaatattga tatttatgag
     1261 caagcgtagg tatggttagc cgcctttagc ggcgtaaccg tacgcatatc agcaaacttt
     1321 ataaaataac aaggccgtct gaaatctgtt tttcaacttt ttcagacggc cttgcaactt
     1381 ggcatttcat tcgtacggtt acgccgctaa aggcggctaa ccgtacctac gagctctgat
     1441 aaaaatgatt tatggaagca agctgtagcc tgcatgaaac ctaaaatcca tgcgtaaggt
     1501 gtgtgctcca gcacgcacgc gttccatgat ttacggctca atgccgtctg aaaagctcac
     1561 aatttttcag acggcatttg ttatgcaagt aaatattcag attccctata tactgcccag
     1621 acgcgtgcgt gctgaagaca cccccta
//
LOCUS       DAWFIT010000161         1612 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFIT010000161 DAWFIT010000000
VERSION     DAWFIT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.161"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..825
                     /locus_tag="WGQ85_002123"
     CDS             <1..825
                     /locus_tag="WGQ85_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0751452.1"
                     /translation="TPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAI
                     WISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEA
                     GAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRIN
                     IANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVV
                     SSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            832..1341
                     /locus_tag="WGQ85_002124"
     CDS             832..1341
                     /locus_tag="WGQ85_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751453.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          517 a          321 c          325 g          449 t
ORIGIN      
        1 acccctacca atgcgaaaaa gcatcaaccg cagaagcccg acaaaaccgc actggaaaaa
       61 atcatccaaa gtattatgcc tgcacatgcg gcaggtgcga tgactaatcc gcaggataag
      121 gatgctgcca tttggataag caatatccgt aatggcatca caggcccgat tgtgattacc
      181 agctatggag tttatgcagc aggttggaca gctccgctga tcggtacagc tggtaaagca
      241 gctatcagca cctgtatggc taatccttct ggttgtactg tcatggttac gcaggctgct
      301 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagctcca
      361 gtaggagcgt tgtcgaaagc gaaggcggct aagcaggcta taccaaccca gacagttaaa
      421 gaacttgatg gcttactaca agaatcaaaa aatataggtg ctgtaaatac acgaattaat
      481 atagcgaata gtactactcg atatacacca atgagacaaa cgggacaacc ggtatctgct
      541 ggctttgagc atgttcttga ggggcacttc cataggccta ttgcgaataa ccgttcagtt
      601 tttaccatct ccccaaatga attgaaggtt atacttcaaa gtaataaagt agtttcttct
      661 cccgtatcga tgactcctga tggccaatat atgcggactg tcgatgtagg aaaagttatt
      721 ggtactactt ctattaaaga aggtggacaa cccacaacta caattaaagt atttacagat
      781 aagtcaggaa atttgattac tacataccca gtaaaaggaa actaactaaa tatgagtaac
      841 tttgaaaaaa aatatatttt agaattaaat gatgctttaa gccatttaaa tcataactct
      901 acctcatttg atttattgaa agttttgatt tcatggttat caaacgatat tgtcattgat
      961 aaatttaaaa ttttaggtta tgactttagt aaatatatcg aaatgaatcc cgatgactat
     1021 ccggttgaaa aatctatatt gaatagagag gaaattattt atctcaaaaa caatatttat
     1081 cgtaaaatat catcaggaaa ttttaaattt caatactttg tacaatatat tagagatatt
     1141 ttagaatatt tatttattga acatattgaa agagtctgtc cttactgcga atggggtgaa
     1201 atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt atctctgtac tcaatgtgga
     1261 tgtgcttttt ataacgataa ttcacaattt ttattaaaaa cccctttaac cattccaatg
     1321 aaacgtgatg aatttaaata aacaagccgt agcctgcatg aaccctaaaa tccacgtgta
     1381 gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg ctcaatgccg tctgaaaagc
     1441 tcacaatttt tcagacggca tttgttatgc aagtaaatat tcagattccc tgtatgctgt
     1501 acagacgcgg gagtgttaag ccccccttgt ttgaagctcc gcggctcctg ccgagcttca
     1561 ccgaccccgt tgtgcccaag ctctctgctc ccggcggcta cattgtcgac at
//
LOCUS       DAWFIT010000162         1608 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFIT010000162 DAWFIT010000000
VERSION     DAWFIT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.162"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(98..1021)
                     /locus_tag="WGQ85_002125"
     CDS             complement(98..1021)
                     /locus_tag="WGQ85_002125"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0751454.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          430 a          369 c          386 g          423 t
ORIGIN      
        1 gggaatgacg aattttaggt ttctgttttg gttttttgtc cttgtaggaa tgatgaaatt
       61 ttaagtttta ggaatttacc ggaaaaaaca gaaagcgtta tccacaattt ctgatgttca
      121 gctcgtgaaa tgcgtcgggc aaatcatcgc tgtcggcaaa ttccacccgg tcgtaagccg
      181 tttcgtctgc caaaaccgcg cgcaagagtg cgttgttgat ggcgtgtccc gatttgtagc
      241 cttcaaacgc gccgacaatc ggatgtccga cgatatacaa atcgccgatg gcatcaagga
      301 ttttgtggcg cacaaactca tcgggatagc gcaagccttc aggattcagg acatccgtgt
      361 cgtcaatcac gatggcgttg ttcaaattgc cgcccaaacc cagattgtgg gcgcgcatca
      421 tttccacttc gtgcataaag ccgaaggtgc gcgcgcgcgc gatttcgtcg atgtaggatt
      481 tgccggcgaa atcaatttca aaagtgggcg agctgcggtt gaaaaccgga tggtcgaatt
      541 cgatggtcag cgttacctta aagccgtcat acggcgtaaa gcgtacccat ttgcccgctt
      601 ctttgatttc gacaggcttg aggattttca aaaaacgctt ttgcgccttt tgatcgacca
      661 cgcccgcatc ttgcaaaagg taaataaacg gcaggctgga gccgtccata atcgggattt
      721 cgggcgcgtt cagctcaatc agcgcattgt cgatgccgta ggcggacagc gcggacataa
      781 tgtgttcgat tgtgccgacg cgcacgcctt tgtcggtaac gatggtggag gaaaggcggg
      841 tatcgttgat caaataaggg gtcagtttga tttgttcgcc catctcgccg tccaaatcgg
      901 tacggcggaa ggaaatcccg ctgttttcag gcgcggggtg cagggtcagc gcgacgcgtt
      961 cgcccgaatg cagcccgact ccggtaacgc tgatggattt cgccaaagtt ctttgcagca
     1021 taaaccgctt ccttatcaat ggggtaagtt ttggaataat acgataaaac cggaaaaaca
     1081 agctatgttt ttccatagta tttgccaatg tatctgtttt caatacgtaa gccgcataaa
     1141 aatgtatagt ggattaacaa aaatcaagac aaggcgacga agccgcccct ctcgaaaaac
     1201 gcaaaaaatg ccgtccgaaa acctttcgga cagcattttc gcgtgaaccg tcattcccac
     1261 aaggacaaaa aaccaaaaca taaaaccaaa aacagaaacc taaaattcat cattcccacg
     1321 aaagtgggaa tctagttcgt tgagtttcag ttatttagaa taaattttga aacttaaatc
     1381 ccgtcattcc cacgaaccta cattccgtca ttcccacgaa agtgggaatc cagttttttg
     1441 agtttcagtc attcccgata aattgcctta gcattgaatg tctagattcc cgcttttgcg
     1501 ggaatgacgg cggcgggttg ctgttttttg tttataaatt tctgtagctt tgaatttcag
     1561 ccatcttcaa taaattatag cggattaaca aaaaccagta cggcgttg
//
LOCUS       DAWFIT010000163         1559 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFIT010000163 DAWFIT010000000
VERSION     DAWFIT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.163"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..836
                     /locus_tag="WGQ85_002126"
                     /pseudo
     CDS             1..836
                     /locus_tag="WGQ85_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            881..1171
                     /locus_tag="WGQ85_002127"
     CDS             881..1171
                     /locus_tag="WGQ85_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751455.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1176..1373
                     /locus_tag="WGQ85_002128"
     CDS             1176..1373
                     /locus_tag="WGQ85_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751456.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          499 a          376 c          331 g          353 t
ORIGIN      
        1 ggttgcggcg tcgcaaaaga aggctgggaa tcacgacttt tcgcattcct gactaatgca
       61 atccgcccaa aaatcacacg cgttgacatc gcaaaagact ttttcaacgg cgaatacagc
      121 ccgaaccaag cccgtgaaga ccgaaataaa ggtatgttta cctgtcatca cgtcaaacca
      181 aaaggcgaat gtttggggtc agattgggaa gaagacgatg aagccaaaat gaccaaaggc
      241 aagacctatg gtatcggctc ccgtgaatcg tccaaatatg tccgcgtcta tgaaaaaggc
      301 aagcagttgg gcgataaaac aagcacatgg acgcgctttg aaattgaatt caaagcaaaa
      361 gacatcgtta tcccttttga agttttgcag aatccgggcg aatatttcgg cggcgcatat
      421 ccgatttgcg aacgattcgt ccaaaaggca acgcgcatac acgcggttaa ggaagataag
      481 gtcatttcag ccgaccgtta ccttgaatgg gtaaaaaaca gttcggacgt gcggcaaacg
      541 gtctgaaatt catttttccc gaattggaca aagccaaact gtttgaactg attgagccga
      601 gtcatcacaa gctgcccaag tctttgactc ccgaagccta cgactgcgcc tttttgaaag
      661 ctcaagccat tcatgaacag cccgcattca aaccgtacaa agacccttac tatatgtacg
      721 aatattacga gaatcttgaa aaacagcttg aacagcaaaa acacgtcaac aatgaagaaa
      781 gctataacaa cttcatttac gacaaattcg caagactacc gatttcatgg gcttaaagtg
      841 tctgcccgaa agacgtttaa tcacacaagg aaaccaaaaa atgaacatcc aacttcaagg
      901 ccacatcgtc ggcgttaaaa aattcaacgg acaaatcgaa ggcaagagct tcgactattg
      961 ccgcctgatt gtcgccacac ccttagacag ctcccaaggc aacgcattgg gcagctctac
     1021 tactgaatac gatttcggcg gctctgccaa tttcgagcag ttccgaaacg cccaatttcc
     1081 catcgaagca aacctgaacg tggaaatcgt tactacgggc aaaacccaaa aactgaaagt
     1141 catcggtttt caactcgtga agaaaggctg attgaatgca gaaagtctat gttgtccagt
     1201 ccgtatcaac aggggacttt ctgtatctct ctcctgaaac gggcgacatc ggacatacca
     1261 aattaatcac caatgccgat tatttctacg acttcgaaga agcgattaac gcaggtttgg
     1321 aagaaatcgg caaccaatac gaatttgtcg tattcggatt tttgaaagac tgattttcgg
     1381 atgttcggcg gtcgtctgaa aaacgctcca tccattaccg ccaaacactt tttgaaggaa
     1441 aatatcatga aatttattaa cacctgccgt aaatacggcg caaaactggc tgttgtaaca
     1501 gctgctcccc tggctttggc cgcacatgca aatgcaacgt tgcccgatac agcaaaaaa
//
LOCUS       DAWFIT010000164         1344 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFIT010000164 DAWFIT010000000
VERSION     DAWFIT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.164"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            29..265
                     /locus_tag="WGQ85_002129"
     CDS             29..265
                     /locus_tag="WGQ85_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751457.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            237..656
                     /locus_tag="WGQ85_002130"
     CDS             237..656
                     /locus_tag="WGQ85_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751458.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            553..858
                     /locus_tag="WGQ85_002131"
     CDS             553..858
                     /locus_tag="WGQ85_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751459.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(903..1073)
                     /locus_tag="WGQ85_002132"
     CDS             complement(903..1073)
                     /locus_tag="WGQ85_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0751460.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1086..1304
                     /locus_tag="WGQ85_002133"
     CDS             1086..1304
                     /locus_tag="WGQ85_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751461.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
BASE COUNT          341 a          310 c          390 g          303 t
ORIGIN      
        1 acctgcaaac caaaagtgtg gaatagcaat gcgtattttg gatattttta aaaacccagc
       61 gacaggcaat gtgtcgcact cgaaactgtg ggcaaacgtt gcctgcgcgg cggggacggt
      121 taagtttgtg atgctgcccg acccgtcggc ggagatttgg gcggtgtatt tgggcattgt
      181 cggcggctat gcggtggcgc gttcgttggt cagcgtcaaa cgtcaggagg tcgagaatga
      241 atctcgtgaa actgctggcg aataactggc aaccgattgc catcatcgcg cttgtcggca
      301 cgctcttggc ggtgtcgcac catcaaggct acaagtcggc ttttgcgaag cagcaggcgg
      361 tcattgacaa gatggaaaaa gacaaagcgc aagccctgct gttgtcggct caaaactacg
      421 cccgcgagct ggaacaggcg cgtgcggaag ctaaaaaata tgaagtcaag gcgcacgccg
      481 tcggcatggc tttggcgaaa aaacaggcgg aagtcagccg tctgaaaacg gaaaataaaa
      541 aggaaatcga aaatgtcctt actcaagacc gtaaaaatgc aggcggcggt tgtattgacg
      601 gctttggcca tcacggcttg cagctctaca agcgcgccct cggctacgga aattaaggtt
      661 gtcgaaaagg cggtcatgcc gacaccgcct gccgcgttga tggtcgcgcc ggtgcgcccg
      721 aatccgccga aagacggcaa gacggccacg ctgttggaac acgccgccga gtttggcggc
      781 tatgttgccg aacttgaaaa ccaaaatcag gcttggcgcg actgggcggg caatcactcc
      841 cgcaaagtcg gaaactgaca aaaaagcccg cgtagggcgc gggctgaggg tgaaagcgga
      901 ttttatacct cttttacagg ggtagcggcg gtagtgcttt tcagcaaatc gactgcgtgc
      961 tggcagtttt gcttgctggt gtagccttcg ccctgagcga tgatttcatg gttggctgct
     1021 ttcaaacgcc aacggtattc gccttttgcg tctttataga tttcaaaata cataaggttt
     1081 ctcctatgaa tgagtacacg ttttcttacc gctttaacgg caagtcctgg tcattgagca
     1141 tttgggcgga caaccctgaa gaagccaggg cgaaatttcg ggctgcacga gaaaatgcgc
     1201 actatgacgg cgaagttgta gcaaaggttt atacatttgt aaatatttcg tgggttaaga
     1261 aattgtacaa gcggacaaaa tatttaatgg gtatcaaaga atgacctacc gtgaattagt
     1321 tgaacgtcag ttggctgtgc gcca
//
LOCUS       DAWFIT010000165         1317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFIT010000165 DAWFIT010000000
VERSION     DAWFIT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.165"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            5..946
                     /gene="mafA"
                     /locus_tag="WGQ85_002134"
     CDS             5..946
                     /gene="mafA"
                     /locus_tag="WGQ85_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0751462.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPSAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKTPDVGNEVIRRRKGG"
     gene            950..>1317
                     /gene="mafB"
                     /locus_tag="WGQ85_002135"
     CDS             950..>1317
                     /gene="mafB"
                     /locus_tag="WGQ85_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0751463.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQG"
BASE COUNT          337 a          507 c          282 g          191 t
ORIGIN      
        1 tgccatgaaa accctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac
       61 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
      121 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
      181 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
      241 tatcgacgca ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag
      301 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
      361 ttccacatcg ctcttaaacg ccccctccgc cgccctgact agaaacagcg gacgcaaagg
      421 cgaacgctcc gccggactgt ccgtcaacgg catgggcgac taccgcaacg aaaccctgct
      481 cgccaacccc cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg
      541 cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt
      601 attcggcacc gtccgcagcc gtaccgaact gcacctctac aacgccgaaa cccttaaagc
      661 ccaaaccaag ctcgaatatt tcgccgtcga ccgcgacagc cggaaactgc tgattgcccc
      721 taaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta
      781 cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacatcac
      841 cccctacggc gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaac
      901 ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct
      961 gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gccgccgcac tcatacagcc
     1021 cgccctcgcg gcggacttgg cgcaagatcc gttcattacc gataacgccc aacggcagca
     1081 ctacgaaccc ggcggcaaat accacctctt cggcgacccg cgcggcagcg tttccgaccg
     1141 caccggcaaa atcaacgtca tccaagacta tacccaccag atgggcaacc tgctcatcca
     1201 acaggcaaac atcaacggca caatcggcta ccacacccgc ttttccggac acggacacga
     1261 agaacacgcc cccttcgaca accacgccgc cgacagcgca agcaaagaac aaggcaa
//
LOCUS       DAWFIT010000166         1164 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFIT010000166 DAWFIT010000000
VERSION     DAWFIT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.166"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..442)
                     /locus_tag="WGQ85_002136"
     CDS             complement(<1..442)
                     /locus_tag="WGQ85_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0751464.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIH"
     gene            complement(562..768)
                     /locus_tag="WGQ85_002137"
     CDS             complement(562..768)
                     /locus_tag="WGQ85_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751465.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
     gene            1058..1164
                     /locus_tag="WGQ85_002138"
                     /pseudo
     CDS             1058..1164
                     /locus_tag="WGQ85_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          293 a          315 c          228 g          328 t
ORIGIN      
        1 aatggataaa accgctcacg tccaacttgt cgcagctgct cagactatcg gtataaacaa
       61 tgctgtccgg catgattttc tttttgatga cagggagtaa cgtttcagac ttggcattgt
      121 ttacgacaac ggtatagacc cgtccgttgc gtttcagaat gccgaagaca accacttttc
      181 ctgccgcacc gcgaccacgt ctgcctttac gccgtccgcc gaaatcgctt tcgtccggct
      241 cgacagagcc ctcaaaaacc tcatcggcag ccaaggctaa atgatggttg ataaccgtgc
      301 ggattttacg gtagaacagt gctgccgaat tgggatggat acccaaaata tcggcggcag
      361 aacgggcggt aacttcgagt acaaaaaaac ggagcagttc tttctgtact tttttcttta
      421 atttgcagtg cgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc
      481 ctagtttaaa gctttgtttc ttaagtccgc agagtatgcc atggttagac cttcaaagtt
      541 gagtattgta ctattttgtt tttaattaac tataaaacgc ttgtgtgaat aggaagactg
      601 cagaatattg ctgccttttc ctgtttgctt taaataaaca gaaaatgccg tctgaaaccg
      661 attttcagac ggcatttgtc ccttaaaaca cgttttttca agcgcgacca taccaaacac
      721 ccacaggttg ccttccaccc ttcgtacaac ctttgctcca tcaaacatcc acttcctaaa
      781 tactgatgtc tgcttaggtt gaatcggcat tcgtacgatt acggcacacc gcctaatctg
      841 tacctgcgtc tgttgcgccc aataccaaag actacctgaa aatctcccaa acttttcagg
      901 tagccttatc acgttttcga ctgttaccac acgcccaaag accgcctgaa accctttttc
      961 agacgttccg ctccgatacc ccccaacaaa acgccgcccg aacgcatgtt cagacggcat
     1021 tttctttgcg tcgattcatt ccccgatata gcgcaggtct ttttccagcc atttcccttc
     1081 caaacaaaaa agcaccgacg gcggccgatg ccccttcctt tacaggttcc cctatttttt
     1141 agctgcgggc agagccggtt ttgc
//
LOCUS       DAWFIT010000167         1074 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFIT010000167 DAWFIT010000000
VERSION     DAWFIT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.167"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            32..>1074
                     /gene="tuf"
                     /locus_tag="WGQ85_002139"
     CDS             32..>1074
                     /gene="tuf"
                     /locus_tag="WGQ85_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0751466.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIA"
BASE COUNT          292 a          298 c          267 g          217 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gcgagaacgt aaccatcacc gtagaactga ttgc
//
LOCUS       DAWFIT010000168         1053 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFIT010000168 DAWFIT010000000
VERSION     DAWFIT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.168"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(124..552)
                     /locus_tag="WGQ85_002140"
     CDS             complement(124..552)
                     /locus_tag="WGQ85_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0751467.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WGQ85_002141"
     CDS             complement(533..967)
                     /locus_tag="WGQ85_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0751468.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          268 a          315 c          188 g          282 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcatcc
       61 cctgcaaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 cttcttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttgccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacccc atcatcccca cccgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc cagcattgcc gctgatgatg tccgacgcag atgcggtttg ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cagaaacatc catatccgca cacgaccgct tatccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc atccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcattat ctctccaata caacatatag tataaattag cgcattttat acaataaaat
     1021 acaatatata gtattaagcc gatgtttttt tgc
//
LOCUS       DAWFIT010000169          975 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFIT010000169 DAWFIT010000000
VERSION     DAWFIT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.169"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            309..>975
                     /locus_tag="WGQ85_002142"
     CDS             309..>975
                     /locus_tag="WGQ85_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0751469.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGI"
BASE COUNT          276 a          205 c          230 g          264 t
ORIGIN      
        1 ggtttgttga aagaaggtgc tcatgagaaa tcccctaaat gtcttggtgg gaatttaggg
       61 gattttgggg aattttgcaa aggtctcaaa cggatgaaat caaccgatat tcaaacacag
      121 tcatttttag cgcattttca gcgtatcgtt aatgcggaaa atttcgtgaa caggtttttt
      181 gcacaggtct ccgcctcgtt tcgcgggatg ggaaaccgta ttagaaaacg gacgcacgca
      241 acataaaccc cgaaagtgat gataagatga tgatttaacg tactgcttta attatttaag
      301 gaattatcgt gtttcccgac aaatacaagt tgagtttgga agaaaatatt tttctggcaa
      361 agaaagtatt ggttgcccaa attcacaacc tcagccgttt tgagaattgt cagacgacct
      421 tgttgcagac cgaacaaatt atccatggca aaaatgtagc ctccgcgtca ctggaagaca
      481 tccaaaccat cttgaacctg aaacgtgcct atcaatatgt gatttcgcat atttcaaacg
      541 gcgaaccggt cgatatttca ctccttaaaa aaatcaacaa cattgttgcc aaggacgatt
      601 ctttggcacc cggtgatttc cgtaccggtt cggtcggcgt aacgctattg gacggttccc
      661 gtcatgcccc aaatccggtg aaggaaattg aagtggctcg cgtgttacaa aatatcggac
      721 tgcaaagcgg ttcgacgacg gaggcagccg tccgttttat gctttattgt atgcggcagc
      781 aggttttttg ggacggcaac aaacgaacgg caaccttatt tgccaacggt ctgatgatgg
      841 cggggggctg cggcatcttg gaaatctccg aaatgcagat gccgcaattc aatgaaaaac
      901 tgtccgcatt ctatcgctcc ggcgacgata ccgatatttc caagtttgtg tatcaaaatt
      961 gtatatcggg catag
//
LOCUS       DAWFIT010000170          969 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFIT010000170 DAWFIT010000000
VERSION     DAWFIT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.170"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            79..>969
                     /locus_tag="WGQ85_002143"
     CDS             79..>969
                     /locus_tag="WGQ85_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0751470.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEHYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVVDATIIQTAGSEQRQAIE
                     VDEEGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDGEGYIEKLHITPANAHECK
                     HLSPLLEGLPEGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRPLSEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKFR"
BASE COUNT          275 a          297 c          215 g          182 t
ORIGIN      
        1 cgagaaaaac ggtttgagac ctttgcaaaa ctccccaaaa tcccctaaat tcccaccaag
       61 acatttagga gatttctcat gagcaccttc ttccaacaaa ccgcgcaagc catgattgcc
      121 aaacacatcg accgtttccc gctattgaag ttggatcagg taattgattg gcagccgatc
      181 gaacactacc tgaaccgtca aagaacccgt taccttcgag accaccgcgg ccgtcccgcc
      241 tacccgctgc tgtccatgtt caaagccgtc ctgctcggac aatggcacag cctctccgat
      301 cccgaactcg aacacagcct cattacccgc atcgacttca acctgttttg ccgtttcgac
      361 gaactgagca tccccgatta cagcacctta tgccgctacc gcaactggct ggcgcaagac
      421 gataccctgt ccgaattgct caaactgatc aaccgccaac tgaccgaaaa aggtttaaaa
      481 atagagaaag catccgccgc cgttgttgac gccaccatta ttcagaccgc cggcagcgaa
      541 cagcgtcagg ccatagaagt tgacgaagaa ggacaagtca gcggccaaac cacaccgagt
      601 aaggacagcg atgcccgttg gatcaagaaa aacggcctct acaaactcgg ttacaaacaa
      661 catacccgta ccgatgggga aggctatatc gagaaactgc acattacccc cgccaatgcc
      721 catgagtgca aacacctgtc gccgttgttg gaagggttac ccgaaggtac gaccgtctat
      781 gccgacaaag gctacgacag tgcggaaaac cggcaacatc tggaagaaca tcagttgctg
      841 gacggcatta tgcgcaaagc ctgccgcaac cgtccgctgt cggaagcgca aaccaaacgc
      901 aaccgatatt tgtcgaagac ccgttatgtg gtcgaacaaa gcttcggtac gctgcaccgt
      961 aagttccgc
//
LOCUS       DAWFIT010000171          947 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFIT010000171 DAWFIT010000000
VERSION     DAWFIT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.171"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            54..416
                     /locus_tag="WGQ85_002144"
     CDS             54..416
                     /locus_tag="WGQ85_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0751471.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            447..947
                     /locus_tag="WGQ85_002145"
                     /pseudo
     CDS             447..947
                     /locus_tag="WGQ85_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          279 a          277 c          197 g          194 t
ORIGIN      
        1 tttttgttaa tccactataa tccgcacaaa tttagtcaat atcaagacca attatgaacc
       61 aactcgacca acttggcacc cgtatcaacc tgatttgcaa tgtcttcgac aaatggatcg
      121 ggcagcagga tctgaattac aacctctttg ccgtacttta taccctggca accgaaggca
      181 gccgcacgca aaagcatatc ggcgaaaagt ggagtctgcc caaacagacc gtttcaggcg
      241 tatgcaaaac ccttgccgga caagggttga ttgaatggca ggaaagcgaa caggaccggc
      301 gcgaacggtt gctgtcgctg accgaaaaag gcaaagccca tgccgcacct ttaacagaaa
      361 gcgcgcagga attcagcgac aaagagacct ttgcaaaatt ccccaaaatc ccctaaattc
      421 ccaccaagac atttaggaga ttttccatga gcaccttctt tcaacaaacc gcgcaagcca
      481 tgattgctaa acacatcgac cgtttccctc tattgaagtt ggatcaagtg attgattggc
      541 agccgatcga acaatacctg aatcgtcaaa aaacccgtta ccttcgagac caccgcggcc
      601 gtcctgccta tcccctgctg tccatgttca aagccatcct gcccggacaa tggcacagcc
      661 tctccgatcc cgaactcgaa cacagcctca ttacccgcat cgatttcaac ctgttttgcc
      721 gttttgacga actaagcatc cccgattaca gcaccttatg ccgttaccgc aactggctgg
      781 cgcaagacga caccctgtcc gaattgctgg aactgattaa ccgccaactg accgaaaaag
      841 gcctaaaaat agagaaagca tccgccgtcg ttgacgccac cattattcaa accgccggca
      901 gcaaacagcg ccaggccaga agtcgatgaa gaaggacaag tcagcgg
//
LOCUS       DAWFIT010000172          933 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFIT010000172 DAWFIT010000000
VERSION     DAWFIT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.172"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>933
                     /locus_tag="WGQ85_002146"
     CDS             <1..>933
                     /locus_tag="WGQ85_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0751472.1"
                     /translation="KQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIV
                     VIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQA
                     AVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSS
                     TGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASK
                     IKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSD
                     AEKHKVISYSKIIAGSVAALNGGDVNTAANAAEV"
BASE COUNT          270 a          226 c          255 g          182 t
ORIGIN      
        1 ccaaacagcc cgaatacgcc tacctgaaac agcttcagac ggccaagaac gtcgattgga
       61 aacaggtgca gctggcctac gacaaatggg actataaaca ggaaggcttg accggagccg
      121 gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga gcggctgcag
      181 cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg acagcggcag
      241 caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc tccttgtata
      301 gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca ttaaaagatc
      361 tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg ggtgcattaa
      421 accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa ctgttcagca
      481 gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac ctcagcaatg
      541 caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa gacaacttag
      601 gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc agcaaaatca
      661 aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg gcgggttgtg
      721 ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca gttggggaaa
      781 tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat gcagaaaagc
      841 ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc aacggcggcg
      901 atgtgaatac tgcggcgaat gcggctgagg tag
//
LOCUS       DAWFIT010000173          918 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFIT010000173 DAWFIT010000000
VERSION     DAWFIT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.173"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(67..846)
                     /locus_tag="WGQ85_002147"
     CDS             complement(67..846)
                     /locus_tag="WGQ85_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0751473.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          229 a          290 c          178 g          221 t
ORIGIN      
        1 cgtactggtt taaatttagt tcactatacc gatacatccg aatatgccag accgtctaaa
       61 cggtattcat acctcccaat cctgcacgcg cttcaaatcg ttttgcgcca aagtatccgc
      121 gtggcggtta cggctctgat attccctgtc tttcatgatg ctgcttgcca cataattcaa
      181 atgtgccttt gccgcctccg aagcctcgcc cggccgacgg ttcgatattg cctcatacaa
      241 tacacggtgc tgcgccatca gcttcggacg cggatcttct tcctgattca gataaataag
      301 gctgctgcgc gtctgccggt acagcatttt caacaaaccg cccgacaaat ggctgaacaa
      361 caaattgtgc gccgcatcgg caatcgtctg atgaaagctg acatcagctt cgctctgatg
      421 ttccaaattg ccgctttcgc acgcctcctc aaacttttca agccaaaacc caatccgctt
      481 caaatcggca tccgtgcggc gttctgccgc caatgccgcc atacagccct cgatgtggca
      541 actgaaatca aaaacatcct gttcccaatt ggaatgcttg cccaaaagct cctgccaact
      601 ttgcaaaaaa tcctgctgcg gcttgaccga aacataataa ccgtctccct gcctcgcttc
      661 caaaacctga cgggcgacca aaacattcaa tgccgacctg accgacgggc gcgaaacgcc
      721 gaactcttcc gccaaaacgc gttcgggcgg aatcttgccc ccttccgcgt aaaccccttc
      781 cgcaatgcgc tcctccaata ccgacaatac ctgatcgctg attttctgag gccttaccag
      841 tttcatcact cctcctttat aaagattccc tgcagaaccc ttccgaaata tagtggatta
      901 acaaaaatca ggacaagg
//
LOCUS       DAWFIT010000174          852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFIT010000174 DAWFIT010000000
VERSION     DAWFIT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.174"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGQ85_002148"
     CDS             <1..318
                     /locus_tag="WGQ85_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751474.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
     gene            355..621
                     /locus_tag="WGQ85_002149"
                     /pseudo
     CDS             355..621
                     /locus_tag="WGQ85_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          220 a          181 c          233 g          218 t
ORIGIN      
        1 catcttcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaat atactatccg cgatgaggcc gattttgcct ggcattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttccg attgggggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatttatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattggat acgattgaag taatgtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggc tgcaggacgg cattatgcgc aaagcctgcc gcaaccgtcc gctggcggaa
      421 gtgcaaacca aacgtaaccg atatttatcg aagacccgtt atgtggtcga gcaaagcttc
      481 ggtacgctgc accgtaagtt ccgctatgct cgggcagcct atttcgggct gctcaaagtg
      541 agtgcgcaaa gccatctgaa agcgatgtgt ttgaaccttt tgaaagccgc caacaggcta
      601 agtgcgcccg ctgccgccta aaaggcagcc cggatgcctg attatcgggt atccggggag
      661 gattaagggg gtgtttgggt agaattagga ggtatttggg gcgaaaatag acgaaaacct
      721 gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggcctca agatatagtg
      781 gattaaattt aaatcagtac ggcaaggcga ggcaacgctg tactggttta aatttaatct
      841 actatatttc cg
//
LOCUS       DAWFIT010000175          811 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFIT010000175 DAWFIT010000000
VERSION     DAWFIT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.175"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..660
                     /locus_tag="WGQ85_002150"
     CDS             <1..660
                     /locus_tag="WGQ85_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0751475.1"
                     /translation="ITHNYPEPTGADKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGW
                     RIAADYASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAASSLGLSAIYDF
                     KLNDKFDKFKPYIGARVAYGHVKHQVHSVEKETEIVTTVPTGGAQTGGTIPQGPTPKP
                     PYHESNSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGSLENTRFKTHEVSLGMRYR
                     F"
BASE COUNT          241 a          235 c          174 g          161 t
ORIGIN      
        1 attacccaca attatccgga accaaccggt gcagacaaag acaaaataag cacagtaagc
       61 gattatttca gaaacatccg tgcgcattcc atccaccccc gggtgtcggt cggctacgat
      121 ttcggcggct ggagaatagc ggcagattat gccagttaca gaaaatggaa caacaataaa
      181 tattccgtca acacaaaaaa ggtgcaagaa aaccaaggca ccagaaaaga cctgaagacg
      241 gaaaatcagg aaaacggcag cttccacgcc gcttcttctc tcggcttatc cgccatttac
      301 gatttcaaac tcaacgataa attcgataaa ttcaaaccct atatcggcgc gcgtgtcgcc
      361 tacggacacg ttaaacatca ggttcattca gtggaaaaag aaaccgagat tgttaccact
      421 gtaccaacgg gaggcgctca aacgggaggc actatcccac aaggtccgac ccccaaacct
      481 ccctatcacg aaagcaacag catcagcagc ttgggtcttg gtgtcatcgc cggtgtcggt
      541 ttcgacatca cgcccaagct gaccttggac accggatacc gctaccacaa ctggggaagc
      601 ttggaaaaca cccgcttcaa aacccacgaa gtctcattgg gcatgcgcta ccgcttctga
      661 ttccccgata ccgatgccgt ctgaaccttc agacggcatt tttgatttac ctgccgttta
      721 cagacgcggg gcgggcgtgg aaatacccga accgtcattc ccgacaacac cgtaatcttg
      781 aaacccgtca ttcccgacaa taccgcaatc t
//
LOCUS       DAWFIT010000176          776 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFIT010000176 DAWFIT010000000
VERSION     DAWFIT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.176"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            80..601
                     /locus_tag="WGQ85_002151"
     CDS             80..601
                     /locus_tag="WGQ85_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751476.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGSYEGYWREKSDKKEGMIVVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCRQTAQAYLDAQKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPRALLL"
BASE COUNT          238 a          157 c          217 g          164 t
ORIGIN      
        1 tttttgcaaa tcaatattga caagctgaaa aaaaaaaaac aatatactca cccagtctta
       61 atgttaacgg agtatggaaa tgaaacaaat gcttttggcc gtcggcgtgg cggcggtgtt
      121 ggcgggctgc ggcaaggatg ccggtagtta cgagggttat tggcgcgaaa aatcggacaa
      181 aaaagagggt atgattgtcg tcaaaaaaga aaaaggcaat tacttcctta ataaaatcaa
      241 cgtggttaca ggtaaggaag aatctatgct tttgtctgaa aaagacggcg agctttcgat
      301 aaacacgggg ataggggaaa tcccgatcaa actttccgac gacgggaaag agctgtatgt
      361 cgaacgcagg cagtatgtca aaaccgatgc ggcgatgaag gacaaaatca tcgcccacca
      421 gaaaaagtgc agacaaacgg cacaggcata cctcgacgcg caaaaggcgt tgccgtcaaa
      481 ccaaacgtat cagcagcgtc aggcggcgat cgagcaattg aaacggcggt ttgaagccga
      541 gttcgatgaa ttggaaaaag aaatcaaatg caacggcaga ccccgggcat tgttgcttta
      601 gcggaggata accgggagga tgccgccgtc cgaatcggac gtgcggtttc cgtaccggta
      661 cgggcgggca ggaatgtccg cctttttttg ttcggacgcg tttgaatacc cgtttgattc
      721 cgaccgtttg caaggggtat ttccgttcgg gcggaaatta tagtggatta acaaaa
//
LOCUS       DAWFIT010000177          759 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFIT010000177 DAWFIT010000000
VERSION     DAWFIT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.177"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..472
                     /locus_tag="WGQ85_002152"
     CDS             <1..472
                     /locus_tag="WGQ85_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0751477.1"
                     /translation="IAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATL
                     MAMLPELGRLSHKRIASLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRF
                     EPLIRDFYQRLLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          179 a          182 c          221 g          177 t
ORIGIN      
        1 gattgcccat tttgaccggc tgattgacga attggacaaa caaatcgacg accacaccca
       61 cacgcatttt gacggcaaag cccaagtggc ggagcaaatc aaaggcatcg gttcgataac
      121 gacggctacg ctgatggcga tgctgcccga attggggcgg ctgtcgcaca aacggatagc
      181 gagtttggcc ggcattgccc cgcacccgag ggagggcggg gaaaccaaat ttaaaagccg
      241 ctgctttggc ggaaggtctg cggtgcgtaa ggcactgtat atggctaccg tggcagcgac
      301 acgttttgaa ccgcttattc gggatttcta ccaacgcctg ctgtccgagg gtaagccgta
      361 taaggttgcc gttacggcat gtatgcgcaa actgctgacg atatcgaatg cccggatgcg
      421 tgattatttt gccgaaaacg ataccgccga aaacggtatc cggacggctt gatttgagtt
      481 ttggtatttt tgcccgacgg ggtgaaaaat acagttgcta cggctcgatg aatcgtcaga
      541 aatacccgaa ccgtcattcc cgcgcaggcg ggaatctaga ccggtcggtg cggaaactta
      601 tcgggaaaaa aggtttctcc gtccctgaat cctggattcc cactttcgtg ggaatgacag
      661 aatgtaggtt cgtgggaatg acgtggtgca ggtttccgtg cggatgaatt catcattccc
      721 gcgcaggcgg gaatctagtc tgttcggttt cagttattt
//
LOCUS       DAWFIT010000178          744 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFIT010000178 DAWFIT010000000
VERSION     DAWFIT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.178"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            62..744
                     /locus_tag="WGQ85_002153"
                     /pseudo
     CDS             62..744
                     /locus_tag="WGQ85_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          217 a          226 c          151 g          150 t
ORIGIN      
        1 gacctttgca aaattcccca aaatccccta aattcccacc aagacattta ggagatgttc
       61 catgagcacc ttctttcaac aaaccgcgca agccatgatt gccaaacaca tcgaccgttt
      121 cccgctattg aagttggatc agatgattga ttggcaactg atcgagcaat acctgaaccg
      181 tcaaaggacc cgttacctct gagaccaccg cggccgtccc gcctatcccc tgttgtccat
      241 gttcaaagcc gtcctgctcg gacaatggca cagtctctcc gatcccgaac tcgaacacag
      301 cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactaa gcatccccga
      361 ttacagcacc ttatgccgct accgcaattg gctggctcaa gacaataccc tgtccgaatt
      421 gctcaaactg attaaccgac aactgaccga aaaaggccta aaaatagaga aagcatccgc
      481 cgccgttgtt gacgccacca ttattcagac cgccggcagc aaacagcgtc aggccataga
      541 agttgacgaa gaaggacaaa tcagcggcca aaccacaccg agtaaggaca gcgatgcccg
      601 ttggataaag aaaaacggcc tctacaaact cggttacaaa caacataccc gtaccgatga
      661 ggaaggctat atcgagaaac tgcacattac tcccgccaat gcccatgagt gcacacacct
      721 gtcgccgttg ttggaaggga tagc
//
LOCUS       DAWFIT010000179          700 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFIT010000179 DAWFIT010000000
VERSION     DAWFIT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.179"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            244..543
                     /locus_tag="WGQ85_002154"
     CDS             244..543
                     /locus_tag="WGQ85_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0751478.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            540..>700
                     /locus_tag="WGQ85_002155"
     CDS             540..>700
                     /locus_tag="WGQ85_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ0751479.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATV"
BASE COUNT          183 a          201 c          142 g          174 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacaag aaaaaactac
      121 accaaactac aatttttgtt catgcaaata tttgttttga caggatttaa acaaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atattgacct aaatattaaa gtttcgtaaa
      241 gtaatgcaac gttgctttaa ttggtttgac cactattgcc gacgattaga aaaatatttt
      301 cggagatgtt caattatgga aacttgggtt caaaactaca cggcaatcgg cggcagcctg
      361 tatctgactg ccgccgccgc actcttaccc atcgtctttt tctttgccgc gctgaccgtc
      421 ctgaagctga aaggctatca ggcggggctt tatacgctgc tgattgcgct tgccgttgcc
      481 gtattcggct tcgggatgcc gacgggtatg gcggtttctt ccctgccgcc gcagccgcat
      541 tgacccaacg cccctacgcc acactgtatt tgaccgcgca ttacaaaatc ggcaaatcca
      601 cccgcatcgg tttggacttt gaaaacgtgt tcaacaaacg ctaccgccct atgcccgaca
      661 ttcacgttta cggcacgccg cgcagcctga ccgcaaccgt
//
LOCUS       DAWFIT010000180          698 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFIT010000180 DAWFIT010000000
VERSION     DAWFIT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.180"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(86..481)
                     /locus_tag="WGQ85_002156"
     CDS             complement(86..481)
                     /locus_tag="WGQ85_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751480.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(465..>698)
                     /locus_tag="WGQ85_002157"
     CDS             complement(465..>698)
                     /locus_tag="WGQ85_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0751481.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          191 a          153 c          131 g          223 t
ORIGIN      
        1 aattctaccc aaatatcccc ttaatcctcc ccggataccc gataatcagg catccggacc
       61 gccttttagg cggcaacagg cgcacttagc ctgagacctt tgcaaatttg tcggtttcgg
      121 ggtcgtattg gtagcctcgt gcctgtatgt cttctttgaa agtttcgtat acgtcatggg
      181 ctaaaagggc tgttccgaca tagggaaccg cccttgtgct gaatttcgcg cctaagcggg
      241 caagtttgcc gccccccgcc aatacgccgg cgcgggaaat gctggcggtt attttggcgt
      301 tgattcgggc ttttgcgccc gtagggatgt gtgttaaatc taccgttttt attaaatcag
      361 atgaataagt tttactattt ttaggtacaa acttatgaat tttcgcacct tgtccggtat
      421 caactgaaac agtttcagat atttttactg catttgcatt cgcttcaaac gaatacatca
      481 tcaaaattgc aattatcgac aatttcgcaa aattcaaatt tgtatatttt atgaccatct
      541 ttcagggatt ctttaattac catttctgaa ttatcagaaa atgagattag ccaaatatca
      601 tgtttaattc ttctattcca gaaaaaagag aaacaatcaa taacattttc agacttatta
      661 atcttcgcaa attcaacaaa ttcagattgc gctataac
//
LOCUS       DAWFIT010000181          682 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFIT010000181 DAWFIT010000000
VERSION     DAWFIT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.181"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>682
                     /locus_tag="WGQ85_002158"
     CDS             <1..>682
                     /locus_tag="WGQ85_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0751482.1"
                     /translation="IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKL
                     DSHKKRKFLGITYDKVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGG
                     AVIRAGVGDQARADAKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFT
                     GPVAPVLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDK
                     WDYKQEGLT"
BASE COUNT          210 a          190 c          159 g          123 t
ORIGIN      
        1 atcgacatca aatctggtgg cagcatcgac gcctacgcca ccgtattcga tgcccccaaa
       61 ggcagcatta acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac
      121 tacgacaaac tagacagtca taagaaacgc aaatttctcg gcattactta tgataaggta
      181 catgatacct ctacccaaca gatgcaaact gccttaccaa gccgtgtagt ggctgaatca
      241 gctaatctgc aatcaggttg ggataccaag ttacaaggta cgcagtttga aaccacgctg
      301 ggcggcgcgg tcatccgtgc aggcgtaggc gaccaggcac gggccgatgc caagattatc
      361 ctcgaaggga tcaaaagcag catccacaca gaaaccgtaa gcagcagcaa atccgccctc
      421 tggcaaaaac aggcaggacg cggcagcaac atcgaaaccc tgcaactgcc aagtttcacc
      481 ggccccgttg cgcccgtact ctctgctccc ggcggctaca tcgtcgacat tccgaaaggc
      541 aatctgaaaa ccgaaatcga aaaactcgcc aagcagcccg agtatgctta tttgaaacaa
      601 cttcaagtag cgaaaaacat caactggaat caggtgcagc ttgcttacga taaatgggac
      661 tacaaacagg agggcttaac cg
//
LOCUS       DAWFIT010000182          654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFIT010000182 DAWFIT010000000
VERSION     DAWFIT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.182"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          170 a          127 c          178 g          179 t
ORIGIN      
        1 agaaaaagaa ccgaaaccga acaagccgga ttcccgcctg cgcgggaatg acgggatttc
       61 aggttactgt ttttgatttt ctgttttgtg gaaataacgg gattttggat gatgtgcatt
      121 tatcgagaaa actaaaaacc ctccgccgtc attcccgcga aagcgggaat ctagaccttt
      181 cggtgcggaa acttatcgga taaaacggtt ttccctgcat cctggattcc cactttcgtg
      241 cgaatgacgg gatataggtt tcactacgga cgtgttcaga ttaccgcctg cgcgggaagg
      301 acgggatttc aggttgctgt ttttgatttt ctgtttttgt gggaataacg ggactttgga
      361 tgatgtgcat ttatcgggaa aacaaaaaac cctccgccgt cattcccgcg aaagcgggaa
      421 tctagaattt taatgctgca agaatttatc ggggatgacc gaggtttgaa ggtctggatt
      481 cccgcctgcg cgggaatgac ggtttagaaa tcacctgaaa cgagaaaaag aaccgaaacc
      541 ggacaagtcg gattcccgca cgggcgggaa ggacggaatt tcagattgtt gtttttgatt
      601 ttctgttttg tgggaataac gggattttgg atgatgtgca tttatcggga aaac
//
LOCUS       DAWFIT010000183          652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFIT010000183 DAWFIT010000000
VERSION     DAWFIT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.183"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGQ85_002159"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGQ85_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0751483.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          116 g          130 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWFIT010000184          608 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFIT010000184 DAWFIT010000000
VERSION     DAWFIT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.184"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..563
                     /locus_tag="WGQ85_002160"
     CDS             <1..563
                     /locus_tag="WGQ85_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0751484.1"
                     /translation="ADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHT
                     HTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESGETKF
                     KSRCFGGRSAVRKALYMATVAATRFKPLIRDFYQRLPSEGKPYKVAVTACMRKLLTIS
                     NARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          160 a          153 c          172 g          123 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatca ggttcacgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacaacca cacccacacg cattttgacg gcaaagccca agtggcagag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgct gcccgaattg gggcggctgt
      241 cgcacaaacg gatagcgagt ttggccggca ttgccccgca cccgagggag agcggggaaa
      301 ccaaattcaa aagccgctgc ttcggcggaa ggtctgcggt acgtaaggcg ctgtatatgg
      361 ctaccgtggc agcgacacgt tttaaaccgc ttattcggga tttctaccaa cgcctgccgt
      421 ccgagggtaa gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctgacgatat
      481 cgaatgcccg gatgcgtgat tattttgccg aaaacgatac cgccgaaaac gataccgccg
      541 aaaacggtat ccggacggct tgatttgagt tttggtattt ttgcccgacg gggtgaaaaa
      601 tacagttg
//
LOCUS       DAWFIT010000185          601 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFIT010000185 DAWFIT010000000
VERSION     DAWFIT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.185"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..581)
                     /locus_tag="WGQ85_002161"
     CDS             complement(<1..581)
                     /locus_tag="WGQ85_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0751485.1"
                     /translation="MQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGGWRIAADYASYRKWNNNKYSVNTKELERNDSNGNWKELKTENQGNGSFHAA
                     SSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVETETTTVFSKPQGTTVPS
                     KIVSKDSSKPAYHESNSIGSLGLGVIAGVGFDIT"
BASE COUNT          123 a          136 c          158 g          184 t
ORIGIN      
        1 gtgatgtcga aaccgacacc ggcgatgaca ccaagaccca agctgccgat gctgttgctt
       61 tcgtgatagg caggtttgct ggaatctttt gatacgatct tgcttggcac tgtagtgcct
      121 tgtggtttac tgaaaacagt cgtggtttct gtttccaccg aatgaacctg atgtttaacg
      181 tgtccgtagg cgacgcgcgc accgatatag ggtttgaatt tatcgaattt atcgttgagt
      241 ttgaaatcgt aaatggcgga taaaccgaga gaagaagcgg cgtggaagct gccgtttccc
      301 tgattttccg tcttcagttc tttccagttg ccattgctat cgtttctttc caactctttt
      361 gtgtttacgg aatatttatt gttgttccat tttctgtaac tggcataatc tgccgctatc
      421 ctccagccgc cgaaatcgta gccgaccgac acccgggggt ggatggaatg cgcacggatg
      481 tttctgaaat agtcgcttac tgtgcttgtg ttgtttgcac cggttgcttg cggataatcg
      541 tgggtaatgc gttcggcggc ataggctaaa tccgcctgca cataatacgg gctgcggctg
      601 c
//
LOCUS       DAWFIT010000186          576 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFIT010000186 DAWFIT010000000
VERSION     DAWFIT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.186"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            1..198
                     /locus_tag="WGQ85_002162"
                     /pseudo
     CDS             1..198
                     /locus_tag="WGQ85_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          140 a          122 c          159 g          155 t
ORIGIN      
        1 acgcaaacca aacgcaaccg atatttgtcg aagacccgtt atgtggtcga acagagcttc
       61 ggtacgctgc accgtaaatt ccgctatgcg cgggcagcct atttcgggct gctcaaagtg
      121 agtgcgcaaa gccacctgaa agcgatgtgt ttgaacctgt tgaaagccgc caacaggcta
      181 agtgcgcccg ctgccgccca aaaggcgacc ggatgcctga ttatcgggta tccggggagg
      241 attaaggggg tatttgggta aaattaggag gtatttgggg cgaaaacagc cgaaaacctg
      301 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctctt ttcgtcattc
      361 ccgccacttt tcgtcattcc cgcgaacgcg ggaatctaga atctcggact ttcagataat
      421 ctttgaatat tgctgttgtt ctaaggtcta gattcccgcg ttcgcgggaa tgacggttca
      481 gttgctacgg ttattgtcag gttttggtta tgttggaatt tcgggaaact tatgaattga
      541 gacctttgca aaaatagtct gttaacgaaa tttgac
//
LOCUS       DAWFIT010000187          562 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFIT010000187 DAWFIT010000000
VERSION     DAWFIT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.187"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(120..326)
                     /locus_tag="WGQ85_002163"
     CDS             complement(120..326)
                     /locus_tag="WGQ85_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751486.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(323..>562)
                     /locus_tag="WGQ85_002164"
     CDS             complement(323..>562)
                     /locus_tag="WGQ85_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0751487.1"
                     /translation="VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPF
                     FISNDTDLALFKTTKWGICRAIADAIAMELGAARV"
BASE COUNT          124 a          160 c          126 g          152 t
ORIGIN      
        1 attggccagt tcgccctccg caaattcaaa cgccgccaag tctgcctgtt cgcttgcctc
       61 acggcttttg gccgaccaca gcccaatcat cttttcgtaa aacgcaacct gtcccatgat
      121 taacgacgat tcttgcgttt gcgcgccgcg cgtttcgcgg ctgccacgcc tgagtttctt
      181 aaatttgggc gtggataggt gtgtatcgga cgaacatttg gcatctgtat aagtgcgcgg
      241 tttacctgct cattactagg attttctcca atcaattttg taagaaaata ggaagcagct
      301 tctttcaatt taccaataat attcatactc ttgccgcccc caattccatc gcaatcgcgt
      361 ccgcaatcgc gcggcagatg ccccatttag tcgtcttaaa caaggccaaa tcagtgtcgt
      421 tgctgatgaa aaaaggctca aacacaatgc cgccggcttg tgcataagcc aggcgcgaat
      481 gctggcccgc attgtcgggt ttaaagccgt cttcgccgcg cagtttccag ccggttttct
      541 tggcaacggc tttgctcaac ac
//
LOCUS       DAWFIT010000188          537 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFIT010000188 DAWFIT010000000
VERSION     DAWFIT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.188"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          155 a          111 c          116 g          155 t
ORIGIN      
        1 tgactgaaac tcaaaaagct agattcccac tttcgtggga atgacgcgat tagagtttca
       61 aaatttattc taaatagctg aaactcaacg cactggattc ccgcctgcgc gggaatgacg
      121 aatttcaggt ttctgctttt tccaataaat gcccccaacc taaaatccgt cattcccgcg
      181 taggcgtgaa tctagacatt caatgctaag gcaatttatc ggaaatgact gaaactcaaa
      241 aaactggatt cccactttcg tgggaatgac gggatgcagg tttccgtatg gatggattcg
      301 tcattcccgc gcaggcggga atctaggtct gtcagtgcgg aaacttatca ggtaaaacgg
      361 tttcttgaga ttttgcgtcc tggattccca ctttcgtggg aatgacgcga ttagagtttc
      421 aaaatttatt ctaaatagct gaaactcaat gaaccggatt cccgcttgcg cgggaatgac
      481 gggattaagt tttcaaaatt catcagaaat tactgattta atagcataaa tttttta
//
LOCUS       DAWFIT010000189          530 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFIT010000189 DAWFIT010000000
VERSION     DAWFIT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.189"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>530
                     /locus_tag="WGQ85_002165"
     CDS             <1..>530
                     /locus_tag="WGQ85_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0751488.1"
                     /translation="ITHDYPKATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGDWRI
                     AADYASYRKWNNNKYSVNTKELENKNNNKRDLKTENQENGSFHAASSLGLSAIYDFKL
                     NDKFKPYIGARVAYGHVRHSIDSTKKTTEVLTSSHAPGTAPTIYNQGSTQNAYHESHS
                     IRRLGLGVIAGVGFDIT"
BASE COUNT          168 a          146 c          109 g          107 t
ORIGIN      
        1 attacccacg attatccgaa agcaaccggt gcaaacaaca caagcacagt aagcgattat
       61 ttcagaaaca tccgtgcgca ttccatccac ccccgggtgt cggtcggcta cgatttcggc
      121 gactggagga tagcggcaga ttatgccagt tacagaaaat ggaacaacaa taaatattcc
      181 gtaaacacaa aagagttgga aaacaagaat aacaataaga gagacctgaa gacggaaaat
      241 caggaaaacg gcagcttcca cgccgcttct tctctcggct tatccgccat ttacgatttc
      301 aaactcaacg ataaattcaa accctatatc ggtgcgcgcg tcgcctacgg acacgtcaga
      361 cacagcatcg attcgaccaa aaaaacaaca gaggttctta cctcctccca tgctcctggc
      421 acagcaccta cgatttataa tcagggaagt acgcaaaacg cctatcacga aagccacagc
      481 atccgccgct taggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac
//
LOCUS       DAWFIT010000190          515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFIT010000190 DAWFIT010000000
VERSION     DAWFIT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.190"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            18..338
                     /locus_tag="WGQ85_002166"
     CDS             18..338
                     /locus_tag="WGQ85_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0751489.1"
                     /translation="MGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGYDEIK
                     AKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLAML
                     KFNS"
BASE COUNT          173 a          102 c          112 g          128 t
ORIGIN      
        1 taatcgacgc atcgggcttg ggcgaaaaaa ttaaagacgg caaaaaaacc gtactttcct
       61 gcgaagaaga acaaaaaatc tgcaatactt tcacgaacaa acaggcagtg gaagatttca
      121 gtgtggtaat cggctacgat gaaatcaaag cgaagaatca cagcttgtcg gcggggcagt
      181 atttcgaggt aaagattgat tatgtggata tttccgccga cgaatttgcg caaaaaatgg
      241 cgggattttc agcggatttg gataaacttt tcgccgaatc tgccgaattg gaaaaagaga
      301 ttaaggatag attggcgatg ttgaaattca attcataaat accgatgaat gaaaccggtc
      361 aaacttaaaa tgccgtctga aagcctttca gacggcattt tgtttggttt ggaaaatact
      421 tatgccgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg
      481 agatttctca tgagcacctt cttccaacaa accgc
//
LOCUS       DAWFIT010000191          508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFIT010000191 DAWFIT010000000
VERSION     DAWFIT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.191"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            323..>508
                     /locus_tag="WGQ85_002167"
     CDS             323..>508
                     /locus_tag="WGQ85_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0751490.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRH"
BASE COUNT          151 a          126 c          124 g          107 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgagctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacac
//
LOCUS       DAWFIT010000192          479 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFIT010000192 DAWFIT010000000
VERSION     DAWFIT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.192"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>479
                     /locus_tag="WGQ85_002168"
     CDS             <1..>479
                     /locus_tag="WGQ85_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751491.1"
                     /translation="DWQPIEQYLNRQKNRYLRDHRGRPAYPLLSMFKAVLPGQWHSLS
                     DPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLELINCQLTEK
                     GLKVEKASAAVIDATIIQTAGSEQRQAIEVDEEGQVSGQTTLSKDKNARWTKKNSLY"
BASE COUNT          135 a          152 c          106 g           86 t
ORIGIN      
        1 tgattggcag ccgatcgagc agtacctgaa ccgtcaaaaa aaccgttacc tccgagacca
       61 ccgcggccgt cctgcctatc ccctgctgtc catgttcaaa gccgtcctgc ccggacaatg
      121 gcacagcctc tccgatcccg aactcgaaca cagcctcatc acccgcatcg atttcaacct
      181 gttttgccgt tttgacgaac tgagcatccc cgattacagc accttatgcc gctaccgcaa
      241 ctggctggcg caagacgaca ccctgtccga actgttggaa ctgattaact gccaactgac
      301 cgaaaaaggc ttaaaagtag agaaagcatc cgccgccgtc attgacgcca ccattattca
      361 gaccgccggc agcgaacagc gtcaggccat agaagtcgat gaagagggac aagtcagcgg
      421 tcaaaccaca ctgagtaagg acaaaaatgc ccgttggaca aagaaaaaca gcctctaca
//
LOCUS       DAWFIT010000193          473 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFIT010000193 DAWFIT010000000
VERSION     DAWFIT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.193"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          153 a           97 c          105 g          118 t
ORIGIN      
        1 gatttttgtt aatccactat aatttattga agatggctga aattcaaagt tacagaaatt
       61 tataaacaaa aaacagcaac ccgccgccgt cattcccgcg aaagcgggaa tccagtccct
      121 tgaccttaag ataatcttta attcgtcatt cccgcaaaag cgagaatccg gaagcgaaaa
      181 gtggtgggaa tgtattagaa atgactgaaa ctcaaaaaac cggattcccg cctgcgcggg
      241 aatgacgatt gatacgtttc gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
      301 ccgcagacag tacaaacagt acggaaccga ttcacttggt gcttgagcac cttagagaat
      361 cgttctcttt gagctaaggc gaggcaacgc cgtactgatt tttgttaatc cactataatt
      421 gatgaaatgc cgtctgaacg ctttgaaaag ggttcggacg gcattttgtt caa
//
LOCUS       DAWFIT010000194          444 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFIT010000194 DAWFIT010000000
VERSION     DAWFIT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.195"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          128 a          123 c           88 g          105 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttctg ttgtccaaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accccccccc ccgaatttgc gtaaaataaa gtgtaatccc tttgattata
      181 agggaatgtt gacaattaga tcgattctcc taggctgact ttcgcaataa atggggttgt
      241 caataattgt gtatggtcgt ttgccgtccg gcggccgtca ttcccgcgca ggcgggaatc
      301 cagcaacaca acgcagcagg actttatcgg aaaaaaccga aaccccaccg accgtcattc
      361 ccgcaaaagc gggaatccag caaccgaaaa accacaggaa tctatcagca aaaacagaaa
      421 ccccaccgcc gtcattcccg cgaa
//
LOCUS       DAWFIT010000195          420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFIT010000195 DAWFIT010000000
VERSION     DAWFIT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.196"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(86..>420)
                     /locus_tag="WGQ85_002169"
     CDS             complement(86..>420)
                     /locus_tag="WGQ85_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751492.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQQ
                     PLLVTPSA"
BASE COUNT          136 a           88 c           74 g          122 t
ORIGIN      
        1 ccttatccca atattaagaa aaacagcaat caaaataaac ttgattgctg tttttcagtt
       61 taagccatga atgtgattag gctccttatg cagatggcgt taccaataac ggctgctgta
      121 cggattgtat attggcatcc acatttcctc ctgtcgcagc agtattagaa ccgaacacag
      181 acatagactg taccaaatta ttgccagcat taatatactt ggcaaaatct ggattactga
      241 tagctgcatc atcaaagaca aatgtatcta cacgatggtt ttcaccatag aagaatccgg
      301 aaatacgtac gttgtcttgt tcagaagcgc taagcaccaa atcacttccg gaacggataa
      361 aatgaacatc tgctgcttta aatcctttaa agtgcatagt gtcagagttt ttatccacat
//
LOCUS       DAWFIT010000196          390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFIT010000196 DAWFIT010000000
VERSION     DAWFIT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.197"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          113 a           81 c           97 g           99 t
ORIGIN      
        1 aatgactgaa actcaaaaag ctggattccc actttcgtgg gaatgacggc agagcggctt
       61 ctgttgctcc cgataaatgc cgcaatttca aatcccgtca ttcccgcgca ggcgggaatc
      121 tagacattca atgttaaggc aatttatcgg aaatgactga aactcaaaaa gctggattcc
      181 cactttcgtg ggaatgacgg gatacaggtt cgtgggaatg acgggatgca ggtttccgta
      241 tggatggatt cgtcattccc gcgcaggcgg gaatctagac attcaatgct aaggcaattt
      301 atcgggaatg actgaaactc aagaaactgg attcccactt tcgtgggaat gacgcgatta
      361 gagtttcaaa atttattcta aatagctgaa
//
LOCUS       DAWFIT010000197          381 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFIT010000197 DAWFIT010000000
VERSION     DAWFIT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.198"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..318
                     /locus_tag="WGQ85_002170"
     CDS             <1..318
                     /locus_tag="WGQ85_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751493.1"
                     /translation="HLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEH
                     TIRDEADFSCHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGMYPHNWGGGNADFSI
                     EYD"
BASE COUNT          101 a           82 c           93 g          105 t
ORIGIN      
        1 catctgcata ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg
       61 caaatcaagc ggcacagcca atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc
      121 ttttgggaac atactatccg cgatgaggcc gatttttcct gccattttga ttatctgcat
      181 ttcaatccgg tcaaacatgg ctatgtagga caaatttctg attggcggtt ttctacgttt
      241 caccgttatg tcaaacaggg tatgtatccg cataattggg gtggcggcaa tgcggacttt
      301 tctattgaat atgattaaag taaagtcgga ttcgagaatc cgacctacgg aaaactgaaa
      361 gagcatcggt tgcagaacgg c
//
LOCUS       DAWFIT010000198          376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFIT010000198 DAWFIT010000000
VERSION     DAWFIT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.199"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           85 c           97 g           92 t
ORIGIN      
        1 gtgggaatga cgtggtgtag gtttccgtgc ggatggattc gtcattcccg cgcaggcggg
       61 aatctagaca ttcaatgcta aggcaattta tcggaaatga ctgaaactca aaaaactaga
      121 ttcccacttt cgtgggaatg acggcagagc ggcttctgtt gctcccgata aatgccgcaa
      181 tctcaaatcc cgtcattcca gcgcaggcgg gaatccagac attcaatact aaggcaattt
      241 attgaaaatg actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgtggtgc
      301 aggtttccgt acggatggat tcgtcattcc cgcgcaggcg ggaatccagg cattcaatgc
      361 taaggcaatt tatcgg
//
LOCUS       DAWFIT010000199          355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFIT010000199 DAWFIT010000000
VERSION     DAWFIT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.200"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT          103 a           97 c           71 g           84 t
ORIGIN      
        1 cattgaaatt ctagattccc gcctgcgcgg aaatgacgaa tccatccgca cggaaacctg
       61 caccgcgtca ttcccacgaa cccacatccc gtcattacca cgaaagcggg aatctaggac
      121 gcagggttaa gaaaacctgc atcccgtcat tcctcaaaaa cagaaaacca aaatcagaaa
      181 cctaaaatcc cgtcattccc gcgcaggcgg gaatctaggt ctgtcagtgc aggaacttat
      241 cggataaaac ggtttcttga gattttacat cctggattcc cactttcgtg ggaatctaga
      301 atctcggact ttcagataat ctttgaatat tgccgctgcc ttaaggtcta gattc
//
LOCUS       DAWFIT010000200          337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFIT010000200 DAWFIT010000000
VERSION     DAWFIT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.201"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(145..337)
                     /locus_tag="WGQ85_002171"
                     /pseudo
     CDS             complement(145..337)
                     /locus_tag="WGQ85_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          140 a           68 c           53 g           76 t
ORIGIN      
        1 cctctaaaaa ataatcgctt gttttgacac cgatacactc atatagtgga ttaacaaaaa
       61 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
      121 gtactggttt aaatttaatc cactatacaa atacagaaac tcaagaaaat aaccttgtgt
      181 attgaccatc tcaagcaatt cagaaaaatc aagaaatttt ctgaccgtaa acaaacgttt
      241 ccctaaaaaa acgatgtctt caaaaatatc gaacaaatag agacctttgc aaaaatagtc
      301 tgttaacgaa atttgacgca taaaaatgcg ccaaaaa
//
LOCUS       DAWFIT010000201          310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFIT010000201 DAWFIT010000000
VERSION     DAWFIT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.202"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            27..>310
                     /locus_tag="WGQ85_002172"
     CDS             27..>310
                     /locus_tag="WGQ85_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0751494.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAAGKAATGIEALSTV"
BASE COUNT           79 a           75 c           93 g           63 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgcgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacaggcaa aggcaatatg ccgctgcgtg aagccgtaaa actgattcgc ggctcggatg
      241 tggcgattga gttccatacc aacgcggcgg ccggtaaagc agctacaggc attgaggcgt
      301 tgagtaccgt
//
LOCUS       DAWFIT010000202          307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFIT010000202 DAWFIT010000000
VERSION     DAWFIT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.203"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT           75 a           83 c           63 g           86 t
ORIGIN      
        1 taaatttata gtggattaac aaaaaccagt acagcgttgg ctcgccttag ctcaaagaga
       61 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactatct gtactgtctg
      121 cggcttcgcc gccttgtccc gatttttgtt aatcctctat accatgtcaa ttcagccttt
      181 atttcgcggg caaagcccac gctacaccca ctttccagaa gtacattagg ctgccactcc
      241 gacaaagccg tttgattccg caaactgtcg gggttcgcca caatctacgg ctactgtgtt
      301 gtgatac
//
LOCUS       DAWFIT010000203          304 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFIT010000203 DAWFIT010000000
VERSION     DAWFIT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.204"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>304
                     /locus_tag="WGQ85_002173"
     CDS             <1..>304
                     /locus_tag="WGQ85_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751495.1"
                     /translation="IAVLDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQN
                     LLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYG"
BASE COUNT           96 a           68 c           58 g           82 t
ORIGIN      
        1 gcattgccgt gcttgatgcc tacacggggc aggattccaa cacactctat tacatgagcg
       61 aggaagatgc gcttaatatc gtcaaagtaa ccaacgatac atacgaccat ctcgccaaaa
      121 acatctacca aaacctgtta ttccaaaccc gtttgcagcc atatttgaat caaatcagtt
      181 tcaaaatgga aaatgatacg ttcactttgg attttagtgg tcttgttcaa gcatttaacc
      241 atgtcaaaga aactaatccg caaaaagctt ttgtggattt ggccgagatg cttgcatatg
      301 gcga
//
LOCUS       DAWFIT010000204          301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFIT010000204 DAWFIT010000000
VERSION     DAWFIT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.205"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            63..248
                     /locus_tag="WGQ85_002174"
     CDS             63..248
                     /locus_tag="WGQ85_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751496.1"
                     /translation="MDGFVVPAKAGIRNQKPQKFIGKTETQKQRKPNGQDSRLRGNDG
                     SYITQKIETKQMGFPLA"
BASE COUNT           87 a           69 c           79 g           66 t
ORIGIN      
        1 ttctgtcctt gtgggaatga cgggatgtag gttcgtagga atgacgtggt gcaggtttcc
       61 gtatggatgg attcgtcgtt cccgcgaaag cgggaatccg gaaccagaaa ccacaaaaat
      121 ttatcgggaa aaccgaaact caaaaacaac ggaaaccgaa cggacaggat tcccgcctgc
      181 gcgggaatga cggctcatac atcacccaaa aaattgaaac caaacagatg ggattccctc
      241 ttgcgtgagg ctgacagatg ccgtctgaaa gactttcaga cggcatagct ttttctcttt
      301 g
//
LOCUS       DAWFIT010000205          297 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFIT010000205 DAWFIT010000000
VERSION     DAWFIT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.206"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT           73 a           72 c           81 g           71 t
ORIGIN      
        1 agtgggaatc cagttttttg agtttcagtc attcccgata aagtgcctta gcattgaatg
       61 tctagattcc cgcctgcgcg ggaatgacgg attttagttt ggggggcatt tattggaaaa
      121 agcagaaacc gctccgccgt cattcccacg aaagtgggaa tccaggatgc agggttgggg
      181 aaaccgtgtt atccgataag tttccgcacc gacaggtcta gattcccgcc tgcgcgggaa
      241 tgacgaacct atccgtacgg aaacctgcac cgcgtcattc ccacgaaagt gggaatc
//
LOCUS       DAWFIT010000206          292 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFIT010000206 DAWFIT010000000
VERSION     DAWFIT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.207"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
BASE COUNT           77 a           74 c           62 g           79 t
ORIGIN      
        1 tttggattcc cgcttgcgca ggaatgacgg tagagcggtt tcagttgctc ccgataaatg
       61 ccgcaatctc aaatcccgtc attccctcaa aaacagaaaa acaaaatcag aaacctaaaa
      121 tcccgtcatt cccgcagctc ttcgtcattc ccgtgaaagc gggaatctag accttttaaa
      181 ttttcacacg gcttttgaat attgccgtta cccgaaggtc tggattcccg cctgcacggg
      241 aatgacggca gagcggtttc agttgtttct aaataaattt ttgcagcgtt gg
//
LOCUS       DAWFIT010000207          267 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFIT010000207 DAWFIT010000000
VERSION     DAWFIT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.208"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..152)
                     /locus_tag="WGQ85_002175"
     CDS             complement(<1..152)
                     /locus_tag="WGQ85_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0751497.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEI"
BASE COUNT           75 a           57 c           60 g           75 t
ORIGIN      
        1 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgcaa agccaaacgc
       61 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      121 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      181 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      241 taaaggtttt gtcggataca agtatcc
//
LOCUS       DAWFIT010000208          239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFIT010000208 DAWFIT010000000
VERSION     DAWFIT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.209"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            36..>239
                     /locus_tag="WGQ85_002176"
     CDS             36..>239
                     /locus_tag="WGQ85_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0751498.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAH"
BASE COUNT           66 a           51 c           60 g           62 t
ORIGIN      
        1 acggcacagg cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc
       61 cgattgaggc gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt
      121 atatgcactt ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat
      181 gtaacgatgt ggttaaaaat ccgcaagtgg aaaaatactt ggcagattat caggcacac
//
LOCUS       DAWFIT010000209          237 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFIT010000209 DAWFIT010000000
VERSION     DAWFIT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.210"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>237
                     /locus_tag="WGQ85_002177"
     CDS             <1..>237
                     /locus_tag="WGQ85_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0751499.1"
                     /translation="AAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGK
                     KSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVS"
BASE COUNT           75 a           55 c           53 g           54 t
ORIGIN      
        1 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
       61 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      121 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      181 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttcc
//
LOCUS       DAWFIT010000210          235 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024728
            SAMN37720384-rid20221643.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFIT010000210 DAWFIT010000000
VERSION     DAWFIT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720384
            Sequence Read Archive: SRR26318878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985352.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985352.1
            Genome Coverage       :: 723x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:18:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 1,977
            CDSs (with protein)               :: 1,977
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 69 of 145
            Pseudo Genes (incomplete)         :: 77 of 145
            Pseudo Genes (internal stop)      :: 13 of 145
            Pseudo Genes (multiple problems)  :: 14 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720384-rid20221643.denovo.211"
                     /strain="HB024728"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1977"
                     /collected_by="Suzhou CDC"
     gene            <1..>235
                     /locus_tag="WGQ85_002178"
     CDS             <1..>235
                     /locus_tag="WGQ85_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0751500.1"
                     /translation="TRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSM
                     EFINGVAAGALNPFISAGEALGIGDILYGTRYAI"
BASE COUNT           62 a           77 c           60 g           36 t
ORIGIN      
        1 acccgcagca tccggcaacg catatccgac aactacaaca acctcggcag caatttctcc
       61 gaccgcgccg atgaagccaa cagaaaaatg ttcgagcaca atgccaagct cgaccgctgg
      121 ggcaacagca tggagtttat caacggcgtc gccgccggcg cgctcaaccc ctttatcagc
      181 gcgggcgaag ccttgggcat aggcgacata ctgtacggaa cgcgctatgc catag
//
